LOCUS       DAOOWJ010000001        85450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_275_length_85450_cov_7.792658, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000001 DAOOWJ010000000
VERSION     DAOOWJ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 85450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 85450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85450
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_275_length_85450_cov_7.792658"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLW22_00005"
     CDS             complement(<1..625)
                     /locus_tag="PLW22_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73109.1"
                     /translation="MRKIFTLLTIATALLMAQCKQPEQKVDVSPISDATVKKVTAELV
                     KQHGDSQKERIERGVAQVAMLWRITDGTETDFEQFCTTQFVSDDAALEQLFNKLSYGF
                     EMLNGYFILLSKELMKPLHLDWGPVTPIDELFGSYNAAAHLNDDLYANKIAFITALNF
                     PYYTLKEKTEMGENWSRKQWAYARMGDMFTSRVPAQLIQNFSTVNTNA"
     gene            760..972
                     /locus_tag="PLW22_00010"
     CDS             760..972
                     /locus_tag="PLW22_00010"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOU73110.1"
                     /translation="MECKKENNSKFCNCSYPCGKHGVCCDCLAYHRKNGELPACYFPA
                     DIERGYDRSIDNFISVVKERGMGYLR"
     gene            complement(1025..2236)
                     /gene="rocD"
                     /locus_tag="PLW22_00015"
     CDS             complement(1025..2236)
                     /gene="rocD"
                     /locus_tag="PLW22_00015"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOU73111.1"
                     /translation="MTPKDYMERESKYGAHNYHPLPVVLERGQGVYLWDVEGKRYYDF
                     LSAYSAVNQGHCHPKIIKAMCEQAQKLTLTSRAFYNNVLGEFEEYVTKYFGYDKVLPM
                     NTGAEADETALKLCRRWAYNVKGIKDGEAKIIVCENNFHGRTITVISMSTDPDSYGGF
                     GPYTPGFIKIPYNDIPALEKALQDPNVAGFLVEPIQGEAGVFVPDEGYLKKAYDLCKK
                     HKVLFIADEVQTGIARTGKMLACDHECVRPDILILGKAISGGAMPVSCVLADDEIMLT
                     IKPGEHGSTFGGNPLAGKVAIAALEVVKEEKLAENAERLGKIFRDEMKNIKSEMIELV
                     RGKGLLNAVVIRNKPGKTAWDVCLAMAEKGVLAKPTHGNIIRFAPPLVITEEQLREAI
                     GLIKEAFKQFE"
     gene            2535..3086
                     /locus_tag="PLW22_00020"
     CDS             2535..3086
                     /locus_tag="PLW22_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU73112.1"
                     /translation="MNEILQSIQQRRSAMAFSPEPIPNEHLIQLFRAAILAPSAYNEQ
                     PWMFFVANRGNTSAFESVLNTLAPANQEWAKSAGALVVTAARKNYTRTGESNYYALHD
                     LGMATAMFLVQAQSMGYVTHMMGGFDHISIRRAIKLDDGYQIGAVIALGKPGSIENLN
                     PQNKQRELSPRIRLDLEKVLRVL"
     gene            complement(3160..3879)
                     /locus_tag="PLW22_00025"
     CDS             complement(3160..3879)
                     /locus_tag="PLW22_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73113.1"
                     /translation="MKKLTLSILILIAALSIKAQEVATTQNKINLNFKVDMVSRYLWR
                     GLLLDNNPCIQPDLSLSVGGFTFGSWASYGYNSKFPEIDFYLIYEFGPLSLTVFDYYN
                     EDETDLSSIKYFTWDRKLTGHALEGTIAWNGTENFPLRALAATFFYGNDRDADGNQYY
                     STYFEIGYPFNLSDYSLEFFLGATPAKGLYNNSANIVNVGFSASKDIKLTENFTLPIK
                     GSFVVNPAQENVFFVVGITLQ"
     gene            complement(3960..6806)
                     /locus_tag="PLW22_00030"
     CDS             complement(3960..6806)
                     /locus_tag="PLW22_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU73114.1"
                     /translation="MKPFLETVAEHLYSTHGNDISKLTLVFPNRRASLFFSKYLSKQI
                     AKPIWLPETTTISDLMFSYAGIKPADPLMLNYKLYKAYCQATNSLEPYDEFYFWGNVM
                     LSDFDQIDKYLVDPKKLFTNINDLKEIEQQFNEFDKETIELIEKFLNLVSSEGESLIR
                     SRYSKIWANLYGIYSAFTNSLISEGIAYEGLAYRLAVKALEGGTSAIPNQKFVFIGFN
                     ALSKSEQRLLLLLKSGERALFYWDYDNYYINSPTGVDHEAGLFMRQNLKLFPNALSNT
                     NFDFIGNIGSSKKVTLISSPSEVTQTKIVPDILDELNINNTNELTTAVVLPDEKLLLP
                     LLTALPEKYSNANITMEYPLRETSAYSFIDALLTLQKSSDKQTKFYYRDVLLIISHPY
                     IQLACPDEANRIRDIIIDRQLLNIPPDTFSGSDILEQIFKPVTKGSELANYLATCINS
                     IISRLTHEIDANDDKLKFEVEYLLTINRALKRLGLVISEINEEYSNKTFRSYLTKALA
                     EQRVSFIGEPLSGIQIMGFLETRNLDFKNLVILSVNDNLLPGSHFSPSFIVPTLRQAY
                     GLPDYKHQNAVYAYYFYRLMQRAENIYLVYTNITEGLKSGELSRFVKQLQMESNLNIK
                     EVTVNFDLGVNSERPIEVTKNEAIAKRLSKYFETTEGSRYLSPSALSEFKNCSLSFFF
                     DRVLGISAPDELDEELDAMGIGSVFHKAMEQIYSGFVGKEVTKDQIQNRLNQLGLVEE
                     TVFRAFKEISKFNGNIDELNGRNRLLLERVNWMVRNALGSDLNRTPYKLLAIEEEVET
                     VIPIELSGKALNVRLGGKIDRLELKNGKLTIVDFKTGKHEPAKTKISNISDFDGKTDK
                     DGLFQQLVYKFIYTQENSLGIGDVDILLWYVRSTDGIPSTEVPDTDEFWEEFISYLKS
                     LIFKIVDDSPSSQTSNIKLCENCSYNVICNRKVM"
     gene            complement(6815..7294)
                     /locus_tag="PLW22_00035"
     CDS             complement(6815..7294)
                     /locus_tag="PLW22_00035"
                     /inference="COORDINATES: protein motif:HMM:NF018596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK family protein"
                     /protein_id="HOU73115.1"
                     /translation="MKRVSILIAITLAAFTAAIAQPKALKNFDELMAALNTGYNVRVV
                     IHYAKCTLVADNELQDKSPDAIGGMTIDTYEYFAAKAVRGNEHAFVVFSENKLIQNPK
                     GKGFVYNYAKFKVEDTGKVKVTVMYLDPNTQEELMSENFFTQFGDSTKEQAIYFYRL"
     gene            complement(7380..8336)
                     /locus_tag="PLW22_00040"
     CDS             complement(7380..8336)
                     /locus_tag="PLW22_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444280.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOU73116.1"
                     /translation="MTKVYPWGDTRRYNSYSGYFKREFGGRVQKLTIDAGFTCPNRDG
                     TVGLGGCTYCNNDAFNPSYCTPQKSITQQIEEGIEFHAVRYRRADRYLAYFQAYSNTY
                     APLEKLRALYSEALSHPKIIGLVIGTRPDCVDEAKLDYFQQLNSKYYIALEYGVESCY
                     NRTLQLINRGHTFEQSVWAINETSKRGIRVGAHFIFGLPGETQEQMLAEADIISKLPL
                     HTVKFHQLQIIKGTIMEQQYTTNPEWFKLFSMDEYFNFLADFIERLNPNFVIERFTGE
                     APPRYLAVPPWGNLRTDQLMIAFEKVLEKRDTWQGRLFESKV"
     gene            complement(8428..9810)
                     /locus_tag="PLW22_00045"
     CDS             complement(8428..9810)
                     /locus_tag="PLW22_00045"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HOU73117.1"
                     /translation="MRKYLIYLTIPAMVACGSKEPKPTTLAGRYTNGTENKVIVISPD
                     AIDTIALNENGLFVHKVTLANPTMLKVRSGRIQTTLYLEPGDSSYFEVDANNADGISF
                     SGSNSELNQNLRSRSKELQNIFRGWRDLFSLNLNEFGLKIDSISKELFQKTDSLKAKS
                     KNFAEMEANRVRYFMLRLRSQYPEYSAYLSGEEFNPDSADYSFFDNVDMNNGEHLSYD
                     EYASLVETYAQLRIQKAQNFKELSSKPAQERLPAIFSMIDSIFTNPKIRDYVKKQYLM
                     EEIQFGEFWKLNDICNNYLAACTTPVYKQEVQSVYNEKLKIAPGNSAPTFTYNDINGK
                     SVSLSDFNGKLVYIDFWATWCGPCRGEIPHLDKLEKDYKGKKIVFVKISLDDDMDAWR
                     KMVTEKKLGGVQLHADGAWSSDAAKNYQIKGIPTFVLIGANGKIINPNAPRPSSEEIR
                     TLLDQELAKI"
     gene            complement(9882..>10256)
                     /locus_tag="PLW22_00050"
     CDS             complement(9882..>10256)
                     /locus_tag="PLW22_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOU73118.1"
                     /translation="ITLDDLDEAMDWAVQNGYITANQRQNVHNSLISMINSEPYKQWF
                     SSTWEVKNEASIVNTDGNIYRPDRVLIGDNQAVIVDFKFGLPTPKHRKQVEQYKDLLL
                     SMGYARVKGYLWYFQLNRHEEV"
     assembly_gap    10257..10356
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10357..12943)
                     /locus_tag="PLW22_00055"
     CDS             complement(<10357..12943)
                     /locus_tag="PLW22_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU73119.1"
                     /translation="MAGLLVYKASAGSGKTYRLVAEYIKMLIENPQAYREILAVTFTN
                     KATAEMKGRIIEKLYKLKRSEEPDLLQTLKAETGIDEDSIRSRAEVALSNILHDYSMF
                     SISTIDSFVQRIIQNLLWELGQHGNNELVLEHKPYIEKASDALLDELTKHPELFNHLK
                     RLIEERLDENKSPNIQNDLINLGNMLFHERYKLLSSEERELMHNKETLNALKAEVNRI
                     ISETYNELKTRAASLLEQIYSSGIHGINQDNYSDFFKKKGGIIPVIAKLAELKRLTPI
                     EYKSIAEDALSDISVWLTKEKISMLKGFVEQHLMPKYRELYSYLNSNYRLYNTALAIN
                     VNISTLYIINELYKKLREKLNDDGVMLLSDSSALLKEFVSQTDTPFIYEKIGTKYTSY
                     MLDEFQDTSQLQWRNFEPLIADSLGSSGLSMVVGDVKQSIYRWRNSDWRIMAGIEAER
                     QFYPQVKQLTVNYRSARTIVEFNNHFFQKAIEINEWETINPQNPAFNLKNLYSDVKQE
                     VKKLEPEGYVEVAFMPPKTDEANDYFTNHIRNILVDLKDRGYRAGDIAFLVRKNEQGR
                     QLAELLLQLSKSDTQLSGFIKIVSQDALILNSSAAVRLCVAALKIALNPKDTLAVAQF
                     RKEFASLKNQHASIHWPSVFLPDSDDTFDWLSSIRFYPLGYMVEAIIQKYFHDQDIDI
                     NDHLPYLSLLHEYAIDFSYRGTSSLSRFIEWYEQDGKGKHLFMAETENAINILSIHKS
                     KGLEFPIIIFPFADLQNQQKQQDSIMWFKLPADTPNEILKNYPLLPIKPKKALAQTYF
                     EADYYDEQFCKEIDDINLQYVAFTRAKHELYILVTDKKLAEDEINKTQENVEQNKFVK
                     FLPNSS"
     gene            complement(13153..15741)
                     /gene="clpB"
                     /locus_tag="PLW22_00060"
     CDS             complement(13153..15741)
                     /gene="clpB"
                     /locus_tag="PLW22_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOU73120.1"
                     /translation="MNLNNFTIKSQEVIQKAVEIAQGYSHQAVEPAHLLRAILDEGES
                     ITGFLLRKLGINPGTFDAVLNRMLDSMPKVSGGEQYLSNTANRVLQKALEFSKAMGDQ
                     FVSVEHILLGLVAAGEQVGQMMKDAGITEKELKQAIAELRKGAKVDSQTAEDTYNALN
                     RFAINLNEMARTGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGIA
                     HRIVNGDVPENLKTKQIFSLDMGALIAGAKYKGEFEERLKSVVKEVIASEGEVILFID
                     EIHTLVGAGKSEGAMDAANILKPALARGELRAIGATTFNEYQKYFEKDKALERRFQSV
                     VVDEPQPADAISILRGLKERYENHHKVRIKDEAIIAAVELSHRYITNRFLPDKAIDLI
                     DEAASKLRLEMNSVPEEIDELERKIKQLEIEREAIKREGDKPKLEELSKTLAELNEER
                     TRLRAKWESEKNVIDQIQQLKQSIEQMKYEAEDAERNGDYGKVAEIRYGRIKQAEAEI
                     EHLKGELKKVQADFALIKEEVDAEDIAEVVSRWTGIPVSRMLQSEREKLLHLEEKLHK
                     RVVGQDEAIAAVADAVRRSRAGLQDAKRPIGSFIFLGTTGVGKTELAKALAEFLFNDE
                     NLMTRIDMSEYQERHSVSRLVGAPPGYVGYEEGGQLTEAVRHKPYSVVLLDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTVDFRNTIIIMTSNIGSHIIQENYAQLSKNNAEQVFE
                     RTRNQVFELLKQSIRPEFLNRVDEIIMFTPLNREEIKQIVLLQFERIKQMLAQNGVSI
                     EISESAVEWITNIGFDPVYGARPIKRVMQKKILNELSKQLLAGTINKEQTITVDYFGD
                     DILVFR"
     gene            complement(16149..16901)
                     /locus_tag="PLW22_00065"
     CDS             complement(16149..16901)
                     /locus_tag="PLW22_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOU73121.1"
                     /translation="MENYFEKQFELRYFEMNKFGEAASTTILTLLEETAADHCYSINH
                     SLYDLEKQNIGWVLLSGIMTIDRYPSYKEKIIIRTWMSSYSMIKGIRENIIYDEKRNI
                     IGKAKGLWVFFDIMRRRPIQIFDSIKERWSYCNDESINHDITKKIKPTDSGIHFKEFK
                     VNRFDVDSNQHVNNIRYLQWLIESIPDYFIDNYYLHSIDGRFIAEAQYGDTILSFTER
                     DISDNSFIHTIKTQKNNKVCATAKTSWKKRIK"
     gene            complement(17102..18025)
                     /locus_tag="PLW22_00070"
     CDS             complement(17102..18025)
                     /locus_tag="PLW22_00070"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOU73122.1"
                     /translation="MRVIVPPIKSQGIKTKLVPWIMDLAPKVSGKWIEPFLGTGVVAF
                     NSGYKKAILNDINPHIINFYKGIQDKTITAPLMKQYLEKEGELLSKAENNGYEHYLKV
                     RTRFNSGEFSPYDFIFLSRAGFNGMMRFGSKGNWNIPFCKKPDRFSQAYITKITNQVA
                     AISKIIQPEPDWTFYNKSFADIIPLATENDIIYCDPPYYGRHVDYYNGWTEQDEELLF
                     NLLSETKAKFILSTWHHNDWRENKMISKFWRKFNIVTKDHFYHNGGKIENRRTVVEAL
                     VCNFDINNFETHNHGLKVQTRPEQLAIHWNE"
     gene            complement(18022..18939)
                     /locus_tag="PLW22_00075"
     CDS             complement(18022..18939)
                     /locus_tag="PLW22_00075"
                     /inference="COORDINATES: protein motif:HMM:NF020794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOU73123.1"
                     /translation="MNLKDKNKYKSDFKKELDKFADKLEKYVSTDNGDWTVKGFIDVY
                     KNIYTISSDTKIVSKILEIHIFPQILQFADSIGYNIILAEKQNWYPDLTFVKKDNEEV
                     KFALDIKTTFRRNDKTAGFTLGSHGGYFKERDKDKNIQFPYNQYTGHYCLGVIYTRTD
                     VSDDLAETEIYQVQELQEEYETPNKKVGERSVTTVKNLKSITSVIKDFEFFAAEKWKI
                     ASDRQGSGNTANIGSVLDIEDLKNENGIFSKLGEEWFDEYWINYGSATIVKDGKPTKI
                     TTLKDFLEFKGRTDLWDKIVSKTSNKKTK"
     gene            complement(19332..19562)
                     /locus_tag="PLW22_00080"
     CDS             complement(19332..19562)
                     /locus_tag="PLW22_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HOU73124.1"
                     /translation="MNIILQSTITCPYCGYSKVETMPTDTCQYFYECTNCGKLLKPKP
                     GDCCVFCSYGSVKCPPLQEQESKGQKGCSCCG"
     gene            19710..21461
                     /locus_tag="PLW22_00085"
     CDS             19710..21461
                     /locus_tag="PLW22_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOU73125.1"
                     /translation="MKWFNRLFISILLLVAAFTAFAQRQVTIKVLQTSDVHGTLFPYD
                     FIRNTPTDGSLARVYTYVKQERNNPNQQVILLDNGDILQGQPTVYYSNFIDSTNPNIV
                     SQMLNYMGYDAATVGNHDIETGPGVYRKVERESNFPWLAANAVSTTTGKPTFKPYVVL
                     ERNGIRIAVLGLITPGIPKWLPKILWPNMQFNDMVESARYWVKYIQKKEKPQVIIGLF
                     HSGHDTSYGGADAAESHNENSSQLVAQQVPGFDMVLIGHDHDRVARKVINANGDSVLI
                     LDPGASARLLSEATITLNLNKRGRVESKSVTGRLVEMKNYEPDPDFMNQFSDFYKRVN
                     DFVSRPIGTITETISSANAYFGPTAFMDLIHDVQLNISGADISFAAPLSFVSKVDSGT
                     ITVSDMFKLYRFENFLYTMELTGKEIDGFLEHAASLWFNTMTGADDNLLRFKEENGRK
                     RLYANYYNFDSAAGIYYTIDVSKPAGDRVTITGMANGLPFDENKTYRVAINSYRGNGG
                     GGHLTEGAGIPHNKLQQRVAKSTDKDLRLYMMKYIEGQGKISPQCHNYWTIVPEPWVK
                     AAMERDKKILFSNRPND"
     gene            21467..22102
                     /locus_tag="PLW22_00090"
     CDS             21467..22102
                     /locus_tag="PLW22_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOU73126.1"
                     /translation="MNFYDTVKQFDDGRMLPLVEEFYTLQGEGYQTGRAAYFIRIGGC
                     DVGCSWCDSKLSWNPEVFPPVRVDEIVERAASFPAKAVVVTGGEPTLYPLDYLCTELK
                     KRGVQTFIETSGAYTLTGQWDWICLSPKRQQPPTEGIHMMADELKVIVSTPADFEWAE
                     ENAAKVKATCQLFLQPEWSVYNQIIPAIVDYIMLHPKWRVSLQAHKFMRIP"
     gene            22212..23453
                     /locus_tag="PLW22_00095"
     CDS             22212..23453
                     /locus_tag="PLW22_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOU73127.1"
                     /translation="MNVSFTILSIGAIIFLAHWFSGIFDRKGIPDVLLLMLVGILFGP
                     ILKLVKPDDFGIAGPMFTSLTLVIILFEGGIGLNISELRKSLGGSLTLTAINFVVTTI
                     AVSILSYMLFHLSVLESLTLGATLGGTSSAVVIPMVRQISMQEISRTYLVLESALSDV
                     LCIVFALAFIEGIVGGEVQPGLIAGKIISSFVLASFVGFASAVVWAVLLNRMRSIKNS
                     IFTTPAFVFIVYGLAEILGYSGAISSLVFGITLVNTEQLRGTFLNKYLPQNPVSLNET
                     EKVFFSEIVFLLKTFFFVYIGISIRFDNLMILGLGLLVALSLYIIRIPVVKLTAHNGI
                     QVADKVTMSVMIPKGLAAAVMAGIPLQKGIPGGQVIQDLTYSIILITIILTSVLIPLL
                     GRYKRLNAIYGWIFRIRFNKR"
     gene            23532..24086
                     /locus_tag="PLW22_00100"
     CDS             23532..24086
                     /locus_tag="PLW22_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU73128.1"
                     /translation="MEQREFYVELLNKHVSNPKMIAHCLASEAVLRELARKFGEDEDV
                     WGIAGLLHDLDVEITNADPKSHALVAAEMLQNILPAEAVDAIKMHNEEATGMPRTTRF
                     QHALAAGETITGLIFATALVYPDKRIASVKPKSVVKRMKEKQFAASVNRDTVMECERI
                     GLSLDEFVQLSLNALIPIAPTLGF"
     gene            24097..24891
                     /locus_tag="PLW22_00105"
     CDS             24097..24891
                     /locus_tag="PLW22_00105"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOU73129.1"
                     /translation="MNIDIVLPQVIGAAQRAGEWALSEREKFTTDMVEVKGKHNFVSY
                     VDKHCEAMLVEALGKALPQAGFIAEEGTGSKNSSGLNWIIDPLDGTTNYIHGLSPFAV
                     SIALMDNDRVVLGVVYEASLRECFWATESGGAFLNGNPIKVTTTQKVNDSLIATGFPY
                     YDYSRISPFFQTMDYFMRNSHGLRRLGSAATDLVYVACGRFDAFYEYGLSPWDVAAGA
                     FIVERAGGKVCDFKGGNSYIFGGEIIATNLNIHQEFTSVIRGIMFG"
     gene            24895..25803
                     /locus_tag="PLW22_00110"
     CDS             24895..25803
                     /locus_tag="PLW22_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU73130.1"
                     /translation="MRKVIHAISFYTFSILTFPFKFMPLRWLYGISDIMYLIIYKIIG
                     YRVDVVKKNLKNSFPQKTESELLEIERKFYRHFCDLFVEQFYILHANAKKAKRLCSFK
                     NLEQLDRYYNENKSIVVAGGHYGNWELYGLFGLFLKHQVFGAYKPLANKYFESFVNAS
                     RERFGALAVPMRDTPRTAMQFAREGKLFFLGLIADQTPAKGEIRYWTTFLNQDTPVFL
                     GTEKIAKKLNQPVFFCNMRKLKRGFYEVELELLTDKPAETKPYEITEMHVRALERLIN
                     EAPEYWLWSHRRWKHSRNLTQTPNEQ"
     gene            25793..26821
                     /locus_tag="PLW22_00115"
     CDS             25793..26821
                     /locus_tag="PLW22_00115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU73131.1"
                     /translation="MSSSKVAVVILNWNGEHFLKKFLPTLIENTPRSQVEIVVADNGS
                     TDGSLLILNDYPTVRTILLDKNFGYTGGYNRALSQVEAQYYVLLNSDIEVPSGWLEPM
                     VTFMDKNPQVAACAPKLIDFNHKEYFEYAGAAGGFIDLFGYPFCRGRILSEIEADNGQ
                     YDDPMPIFWASGACMMVRSSCFWEAGGFDDDFFAHMEEIDLCWRLQNRGYIIYSIPSS
                     VVYHVGGGTLPNNNPFKLFLNYRNNLSMLHKNLSLRALLVVIPVRMVLDILSAFVYLV
                     NGRLSFTNAVFKAHKQYLANISTLNKKRRLLKPFNQATRYRGLILVDFFILRRRKFYS
                     LPISGNNY"
     gene            26979..28664
                     /locus_tag="PLW22_00120"
     CDS             26979..28664
                     /locus_tag="PLW22_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU73132.1"
                     /translation="MVSFKKLTIRDILFRSRMAYSNKPSLAFVGQEPVTYADMAVIVR
                     NVSGMLNTLGIGKGDKVALIGENSPNWGMAYLAITSMGAVVVPILPDFSGHEMESIIN
                     HSEAKVVFVSAKLYGQLDRKNIPGVIAYILINNLQPFDESVTTPTAGMPYETPKAIVN
                     VDYCDCELPRPDEDDLAAIIYTSGTTGRPKGVMLTHKNLVSNVLSTLMIQEVNENDRL
                     LSILPLSHTYECAIGFLIPMATGASVYYLDKPPTPAVLIPAMQAVKPTMLLSVPLVIE
                     KIYKNKIKPELTSSSLKRKLYSFTPTRKLLHRIAAKKLHKTFGGELHFFGIGGAKLSF
                     EAERFLNEGRFPYSIGYGMTETSPLLTGSSPKLVRFRTAGFSIPGQELKLLNPNPHTG
                     EGEIIVRGANVMVGYYKDPELTQTMFIDGWLRTGDLGYIDSDGYVEIRGRSKNMILTA
                     SGENIYPEEIEDVINKHEFVLESLVYELRGKLVARVHLNKEALEKYYENLKHSAKDWQ
                     HNAEDHTRKVLDDIMTYVNAKVSKFSRLNHIVEQQEPFVKTPTQKIKRFLYKE"
     gene            complement(28749..29888)
                     /gene="lysA"
                     /locus_tag="PLW22_00125"
     CDS             complement(28749..29888)
                     /gene="lysA"
                     /locus_tag="PLW22_00125"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOU73133.1"
                     /translation="MQTPFYQYDLALLNETLNAAAAQAARYGYTIHYALKANANPALL
                     KIISGAGFGADCVSGNEIKVALKNGFKPQGIVFAGVGKTDWEIEYAIEQNIQCIHCES
                     VQEFQVIEQIAQKLNKTASIALRINPNVDANTHKSITTGLKENKFGMSAEEASLILNS
                     ANKYKNINLVGLHFHIGSQIMNMEVFKNLALEVNRIQERLQSKALPYLNLGGGLGIDY
                     INPRINRIADFAGYFKTINQWLNPGKEQTIHFELGRSLVGQCGQLYTRVLYTKTTKSR
                     NFAIVDAGMNALIRPALYGAIHRIENISKIEYPTIATYDVVGPICETTDCFGEQVNLP
                     NTERGDLLVIHSCGAYAESMASDYNLRERPKSVFNHSGLALLPKH"
     gene            complement(29891..31252)
                     /locus_tag="PLW22_00130"
     CDS             complement(29891..31252)
                     /locus_tag="PLW22_00130"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU73134.1"
                     /translation="MKVLKFGGTSVGNADRLRKVSELLNGDEPIVVVLSAMAGVTNSL
                     VEITELAQSGKMLEAIEKTKELEDKHIKTADRLFDTKMYRKRGEEFISNLFLRILSRL
                     IEGFNQTDSRNIVALGEQLSVGLFHMLLTEQGKRAAYIPALEYMRIDKDNEPDFFYIE
                     QNLKRLMQNNPFAKIYVTEGFICRNANGLVDNLGRGGSDLTAAIVGNVLNANEVQIWT
                     DIDGVHNNDPRFVTNTFPLRQLSYDEAAELAYFGAKILHPQTIQPCKVKNIPVVLKNT
                     LNLTDEGTVISSIKNRKGIKAVAAKDGITAINIKSSRMLLAYGFLHRVFEVFDTYKTP
                     VDMITTSEVSISLTIDSTINLEKIVNALSQFAQVEVERNQTIVCVVGDFPASHKGYAA
                     MVFDCLREIPIRMISYGGSVNNISFLIDSHYKVQALRLLQQLFPAVSVSDSTHQNIEA
                     NTF"
     gene            31642..34095
                     /gene="pheT"
                     /locus_tag="PLW22_00135"
     CDS             31642..34095
                     /gene="pheT"
                     /locus_tag="PLW22_00135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU73135.1"
                     /translation="MKISHSWLTQYLNVSKTPDEISKILTSIGLEVESLEAKETIKGG
                     LAGVVVGEVLTCEKHPDADKLSLTTVNVGVDQPLSIVCGAPNVAKGQKVLVATVGAKL
                     YFNDQEIVIKKAKLRGQLSEGMICAEDELGLGDSHDGIMVLPADAPVGMPAADYLRIE
                     KDYQYEIGLTPNRIDAASHYGVARDLAAYLTYHESPVKANLPDVSNFKVDNNTLPIPV
                     EVLNTEACPRYCGITINGVKVAPSPEWLQKRLRSIGLNPINNVVDVTNFVLHEVGQPL
                     HAFDADAIKGNKVIVRTMPEGTPFVTLDGVERKLSADDLMICNESEPMCIAGVFGGLH
                     SGVTEKTTKVFLESACFNPVYVRKTGRRHDLHTDASFRFERGTDPNITVWALKRAALL
                     IKEVAGGTISSEIVDVNSGPVNHFEVDFNIHRAWSLIGKQIPEVDIERILEGLEIRVL
                     KKNGQQWLLGVPTYRVDVRREADVVEDILRIYGYNNVEIGAKVNGTLSYTKKPDSLQM
                     QNIVSDYLSSNGFAEIMCNSLTKGGYYEGLATFPEVNSVKILNPLSNELNVLRQTLLF
                     GGLESIQRNINFRNLNLKLYEFGNCYVFKPEVKSQKPLDKYSEHFSIGIWLTGDNIKE
                     HWMTKPERQNFYHLKGYVHSILSRFGFSIDRIEASEAPGDIYAYGLTYKAQGKHLVTY
                     GMVSPEILRQFDIKTEVFFAEIMWLPLMDMIAESKIQYQELPKFPEVRRDLALLIDSS
                     VKYEQIRKLAFETERKLLKSVNLFDVYQGKNIPEGKKSYAISYILQDESKTLTDEQID
                     RTMKRLIDVYVRELGAQIR"
     gene            34105..34611
                     /locus_tag="PLW22_00140"
     CDS             34105..34611
                     /locus_tag="PLW22_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOU73136.1"
                     /translation="MAKIELVLGDITQMDVDAIVNAANESLLGGGGVDGAIHRAAGPQ
                     LLEECRTIGGCPTGEARITKGYNLKARYVIHTVGPIWRGGQHNETLLLRHAYVNSLKL
                     APKHGLKSIAFPNISTGVYHFPKQSAAQIAIRAVMEFTAKNHLPERIVFVCFDQENYS
                     IYSELLGK"
     gene            complement(34614..35996)
                     /locus_tag="PLW22_00145"
     CDS             complement(34614..35996)
                     /locus_tag="PLW22_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU73137.1"
                     /translation="MMKEISILVLDDEKVFRKEIKEFLDGEGFSVLTAERPSEAFSIL
                     SENQIDILILDLRLPEMDGLQVLTKVKEEYPDIEVIMITGHGDMDAVINAMRLGAIEF
                     FPKPFRLLDMRAAIQRTKRYIALHEQLKEINQTYSLVSKDLIESIGTEIIGNSFGIRQ
                     VVDLMGKVAKTDNTSVLITGESGTGKELVARGIHYLSSRKKSYFYAVNCSAIPDTLFE
                     SEFFGHKKGAFTGANEDKAGWFEVANGGTLFLDEVVEMQPAMQSKLLRVLEERKIRRI
                     GSSVDIPVDVRIIAATNQDINKLIEEGKFRSDLYYRLNSFQIHIPPLRERKVDIPILF
                     DYYVKHISTRMGKKITQVDPTIVKAMMGYNFPGNIREMRNMVERAIILSEGGRLTMKN
                     FSGLIAPTDSVSTTGIIGEDIFDLDLIEKIVIMKALERTGYKKTEAAQLLNITRQALD
                     RRLEKYDINL"
     gene            complement(36018..38252)
                     /locus_tag="PLW22_00150"
     CDS             complement(36018..38252)
                     /locus_tag="PLW22_00150"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73138.1"
                     /translation="MKHLLRIVWLTLSLAGLAQIGLSQTDTLPQEIAVKGSSKAADLV
                     VQGKSLLKTNPDRCLDIGLQALVTAKKEGDSFTESMALELLAEANYKLGEYETSLRYF
                     NRAANLYSSINKPEFAAKSLLGMARVYEASQNLDLAIQQLEAGIKTLKNIPNKNVYLE
                     LILKLGNLHLSQKNFKQAKQYGIEALTLLNNQATSVYNKNKALIDTYTLLGQASKNSG
                     DLGTSLDYFKKVSEAAIKQSDSTSYSKSLNEIGVVFMLTQKYDSAYQYFNLSYTISLN
                     ASDTVGIIQSLQGLGDYYYEKGNYNQAINYYTQCQSISSKYNDIPTLIAALVSISRCH
                     FQLGDYPTSSRFLNQALNIAQKNNLTSSKADVYRYLSVINEAQGRYKDALDYHKMWVD
                     LRDSLQYEETGQRLAKLQILYEISQKEKENEILKQNSEIQKLQIAKSRYQMRFLISML
                     VVVAIILSLLVVLFRNKQKEIAKQKETEQRITEINRELERRMIEEIKKQEKQQQLLSQ
                     KSKLESLGTLAAGIAHEINQPLGGISMGLDNILMRIQDKNCSEEYLKEKLNSLFENVE
                     RIKKIIDHIRYFSRTQKPVSFSTVNLNDVVKSALFMVSAQFENHGVIIETSLDENIPP
                     ITADKYKLEQVLLNLLSNAKYAVDEKEKKSEDKTYRKKIKIGTRKDEQNLYLTVWDNG
                     TGIPQKIVEKIFDPFFTTKSEDKGTGLGLSISYGFIKDLLGDIRVESEPNEYTLFEIS
                     IPRE"
     gene            38328..39191
                     /locus_tag="PLW22_00155"
     CDS             38328..39191
                     /locus_tag="PLW22_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOU73139.1"
                     /translation="MFFKVKNYLSLVKFSHTIFAMPFALIGYFLAYYKHSLNPDWWLF
                     LKVILCMVFARNAAMSFNRLVDRHFDKLNPRTSGREIPRGIIAPRSVMLFCIVNSILF
                     ILTTFFINPLVFYLSPIALGVILFYSYTKRFTMFSHLVLGIGLSLAPIGAYLSVTGSF
                     ALIPILYSVVVLFWVAGFDIIYALQDEEFDRNSKLHSIPAAVGARGALVIALVLHFMS
                     ALIVIAIGILAGFNIYYWIGAFLFIALMIYQHYLVRPTDISKVNMAFATTNGMASLLF
                     ALFNIISILTC"
     gene            39361..39786
                     /locus_tag="PLW22_00160"
     CDS             39361..39786
                     /locus_tag="PLW22_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73140.1"
                     /translation="MKKYIVFFGLLSLILGCSGNQNSANKVELVDGQPVIDVTVDSLL
                     LNAKNYESKVVRVSGIVEHVCVHTGLRITLQGKSPEMKLSVKTSDASRPFDMSMKGAK
                     ATVTGVFKRVGGANEVCDVESAHHPGEIYFLECNSINVK"
     gene            39789..40340
                     /locus_tag="PLW22_00165"
     CDS             39789..40340
                     /locus_tag="PLW22_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HOU73141.1"
                     /translation="MTLSKFLRVIHRDCSYFFAGMVLLYSVTGILLNHGHQLNPYYYI
                     DVKTVKVDIPRDSSKVNDQFLLGVFSPFGESEIRSHYYPDSSTLKVFFKNGTATVNLN
                     EQTALIENSIRRPILYQLNMMHRNRPKAWWTWFSDIFAVGLIAITLTGLFLIKGKNGI
                     TRRGAVLVAAGLILPILAMIIFF"
     gene            41147..42523
                     /locus_tag="PLW22_00170"
     CDS             41147..42523
                     /locus_tag="PLW22_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOU73142.1"
                     /translation="MELPFAESYKIKMVEPIRRSTRQEREQWIKEAKYNLFNLPSNQV
                     YIDLLTDSGTGAMSDRQWSAIMLGDESYAGASSYFNLKNAIRNILGFDYIMPTHQGRA
                     AENVLFSVLVKEGDYIPGNSHFDTTKGHIEFRRAHAVDCTIDEAADTELIHPFKGNVD
                     PQKLDNFLKEHSQRVPFVIVTITNNTAGGQPVSMKNLREVREICNRYGKLLVFDSARF
                     AENAYFIKTREEGYAHKSIKEIVREMFSYADAMTMSSKKDAIVNIGGFIAMRDQELWR
                     QCSKFNIMFEGYVTYGGMSGRDMNALAVGLDEGTEFDYLETRIKQVEYLGNRLDEFGI
                     PYQKPAGGHAIFVDANKVLPNIPREQFRAQTLAIELYLEAGVRGVEIGTLLADRDPET
                     RENRYPKLELLRLAIPRRTYTNNHMDVVAVALKNVYDRRNQITKGLEIVWEAPIMRHF
                     TVELKPAE"
     gene            42747..44126
                     /locus_tag="PLW22_00175"
     CDS             42747..44126
                     /locus_tag="PLW22_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU73143.1"
                     /translation="MKDFTTGNEAKHIFRFALPMLIGNIFQQLYNVVDSIIVGRYLGD
                     KALAAVGASFPIIFMVIALIIGIGIGSSVVISQYFGAKDFDRVKRAADTTYIFLFFAG
                     IIITIVGLLSGEYIFRLMRLPEEILPMAKTYLDIYMYGLVLMFGFNSVSSILRGVGDS
                     KTPLYFLIVSTFLNIFLDTLFVLGFKWGIAGAAWATVIAQAFSFIIAVIYLERLRHML
                     RITFKRLVFDWEIFWQSVRIGLPTGIQQTLVALGGMALMGIVNTFGTEVIAGFSAASR
                     IDSLAVIPIMNFSASLSGFVGQNIGAGKVDRVKRGLWATIKMSVLFSVIITAIILIWG
                     DKMMVVFTTNPRVVDIGYHYLMVVSFFYVAFAVMFCINGLLRGAGAAVVPMYITLLSL
                     WIVRIPLAYLLSKTFGMNEAGVWWSIPIGWLMGAVASTIYYRYGSWKNKSVVKPVTTE
                     DDGVATDLV"
     gene            44195..44479
                     /locus_tag="PLW22_00180"
     CDS             44195..44479
                     /locus_tag="PLW22_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73144.1"
                     /translation="MASKRDLKKDIDYLVGEVISDCYTYMLINGEKKRDDAIAIIESI
                     LEKRNDLIHRIKHPAKKDDRKAVKAHYREIQKDALKAIDESFSKLSELTK"
     gene            44600..46564
                     /locus_tag="PLW22_00185"
     CDS             44600..46564
                     /locus_tag="PLW22_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOU73145.1"
                     /translation="MIQEFRQYFSDITLKGNIYLMLLWRFALLMLIFTLCRVLFYLFN
                     IDFFASVTTAGLLRMLAGGLWFDTSALIYTNILYIVLFLLPFTFRYNEWYQRVLKYLF
                     VVTNSVAIGANCADFIYFRFTMRRTTATVFSEFKHETNFGSLIPKFIADYWYVFLIWI
                     ALTALLVLLYGRVRPINKGKGFRFHLVYGLNGLVLLLVFATLMVGGMRGGFRHSTRPI
                     TLSNAGQYINEPLEAAIVLNTPFAIYRTLGKKSLVKVDYYKNPKELDEVYSPLHKPQV
                     TDSLKRMNVVIFILECFGREYIGAYNKHLKEKDGYTGFTPFLDSLIGHSLWFTHSYGN
                     GRKSIDGMPSVLASIPMFVEPYFLTSYSTNKINSIASALRAEGYHTAFFHGAPNGSMG
                     FQAFANVAGFEEYYGMSEYPDPSHFDGMWGVWDEEFFQFFAQTLNKFPQPFCAALFSV
                     SSHHPFKVPERYEGVFPKGTLPIHQCIAYTDYSLKRFFETVSRMDWYPNTLFVITADH
                     PNQTQFPEYQTSVGAFAVPLIFYRPDNSLAGQKDEFAQQIDIMPSVLGYLHYSKPFIA
                     FGRDVFDSTSKPFVVNYINNCYQYYSDDYVLIFDGKKTLGLYNIKDDTMLTNNLQGQL
                     PDRVNEMESKLKAIVQQYNSRMIDDNLVVK"
     gene            complement(46568..46999)
                     /locus_tag="PLW22_00190"
     CDS             complement(46568..46999)
                     /locus_tag="PLW22_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73146.1"
                     /translation="MQTKIIHILLFSILVFTNTACDDNIDDIIPDVPVNIEFNLNEPQ
                     FQNLNIVTGAVKIDGYGYRNNGVIVFRLSENEILAFDATCPKHIDINASVNPDSNGSG
                     TATCPHCGTIYYLVNYGYPKEGYPLKRYQVLKSGDYVRVYN"
     gene            47020..47400
                     /locus_tag="PLW22_00195"
     CDS             47020..47400
                     /locus_tag="PLW22_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73147.1"
                     /translation="MDKGIKKILFALIALFVLVGCGKQLAPSPVPATPGRSKWQVKRE
                     EYRRAWVAYWHDRKTERLTRGAKAKSVKREREAEKAEKKLAERHLKKQPPEVQERMKA
                     SLKEAEKNRPKRTLRQRLGFWKYK"
     gene            47414..48025
                     /locus_tag="PLW22_00200"
     CDS             47414..48025
                     /locus_tag="PLW22_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73148.1"
                     /translation="MDAGDFIYILIAIALAILNAFANSKKKKAAEQKRQAQTSAPADD
                     DLITKKLQELLGQEVIVENEEQPLESTVVNEEEKYIYEYSSKPEVETLDRSVEFEKEP
                     IDKPLTEEELYPIQPQKPIDTSTPIEYTPIDIAESKVEGPIGDYSYQDSFNESMHEVD
                     IDASDAEELESVKKVEEEKEPLLADFDPLKAVVYAEIIRPKYF"
     gene            48133..48606
                     /gene="greA"
                     /locus_tag="PLW22_00205"
     CDS             48133..48606
                     /gene="greA"
                     /locus_tag="PLW22_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOU73149.1"
                     /translation="MSKVTYLTEEGLQKLRKELEQLKTVERPAISRMIAEARDKGDLS
                     ENAEYDAAKEAQGMLELRISKLEDLIANARIIDESKIDTTQVQILNKVKLKNLKTNAV
                     VEYTLVAESEANLKENKLSISTPIGKALIGKKVGDVVDVQVPSGLMKFEILGISL"
     gene            48618..49013
                     /locus_tag="PLW22_00210"
     CDS             48618..49013
                     /locus_tag="PLW22_00210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOU73150.1"
                     /translation="MATIFSRIVSGEIPSYKVAEDERFYAFLDINPLAKGHTLVIPKV
                     EVDYLFDLDPETLAGLTLFAQRVAKAIERVVPCKRIGVAVLGLEVPHAHIHLVPLNKE
                     SDIDFHKPKLKLTPEEFIELAKSIAAEFK"
     gene            complement(49074..50906)
                     /locus_tag="PLW22_00215"
     CDS             complement(49074..50906)
                     /locus_tag="PLW22_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOU73151.1"
                     /translation="MRKHLSFSLRYFVFWVILFSTYRIAFLIYQFENLKSVKFNEFIN
                     IFIRGAWMDSSLAGYILLLTFLLLAIFFWVSPKSITRMYNALTILLLLVVNTVVISDM
                     ELYRNWGYRIDATPLLYLKTPKEAMASVKTPLLILFVVLLIGITYLTHLAYRKWVARE
                     LSQANKARWWYSPIYIFLAATMIIPIRGGFGIAPINPGKVYFSQNNFCNHAALNPVWN
                     MMYSVSKSSSMSKTYNVEIDKATAHQRFGELMHTDSTLYVLNTNQPNIIIILLESFGA
                     KLVGPLGGLPNVTPNLNRLASEGILFTRTTASGDRSDKGIIAVLSGFPAQPTQSVIKY
                     PTKSNKLPTISEVLYNQGYSTAFYYGGDPDFANIRSFLYHAKFQRLITQDDFPKSYRN
                     SKWGVHDEHVFNYLLTDIDSAKAPFFKMFFTLSSHEPFEIPAKPKFNGKTEVEQFLSS
                     AYYADSCLGDFIAKAQQREWFKNTLIVLIADHGHRHPANHPNWEPLKFGIPMVWLGGV
                     VERKPLVVTTTCSQTDLATTLLAQLKLPHDQFTLSKNILASKVTPFAYFAFNEGFGYV
                     NQTDTIVYDLVGKKYIKQTGKNISETRNDAWAFFNAYQEVFQGL"
     gene            50965..51915
                     /locus_tag="PLW22_00220"
     CDS             50965..51915
                     /locus_tag="PLW22_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOU73152.1"
                     /translation="MRKKILVLLFIVGFVVSNSFPQTWSIAVHGGAGNMKPENFTKEQ
                     LDEYEREMKAALGIGVKILNSGGTSLDAVEQVVRYLEDCPLFNAGRGAVFTHEGRNEL
                     DAAIMDGKTLMAGSVAGVTDVKNPISLARKIMEDSPHVMLAGKGASEFAKVKGIEIVD
                     TSYFFTPQRWNDLQRTLSKEKMGTVGCVALDVHGNLAAATSTGGMTNKRWGRIGDAPI
                     IGAGTYANNSTCAVSATGHGEYFIRFTVAHDISALMEYQSLSLNAAVERVIMGKLLNA
                     GGRGGAIAVDRSGNVCMMMNTTGMFRAFARSDGSSGFAIF"
     gene            52013..52528
                     /locus_tag="PLW22_00225"
     CDS             52013..52528
                     /locus_tag="PLW22_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73153.1"
                     /translation="MALEVHVKSAESKGHVEIFLLHFVLMLIAMFVFLALIMLIGGSF
                     TWPNFWKFYITFIPLTVILPVIVAIATSVSYKRVNVHILPASKVNIEKLKDFMFSEDY
                     IIVDKDKTFIRFIRRKNLPRLLSLNFDKPSITIQNDEVIVNVLKRLSLVLLPQLTIGK
                     KFIIEPESSKE"
     gene            52525..53625
                     /locus_tag="PLW22_00230"
     CDS             52525..53625
                     /locus_tag="PLW22_00230"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOU73154.1"
                     /translation="MIKGFPYLLIDIPKCEQNIAFMVQKATKAKVLLRPHFKTHQSLA
                     VGELFRKQGVNRITVSSVAMAEYFAAGGWNDITIAFPFVPQMAQRVNALALGIDLNVT
                     FSSPQNLLATIDRVESKVGVFVEVDVGHSRSGVAVDNTRELALMVNLISSKPNLVFKG
                     FLTHAGHSYSARGREEVDKLHSEALSKLAKLRSFWVDQFPDLTISYGDTPTCSVSDEF
                     WGIGEIRPGNFVFYDVMQYQIGSCTTSQVAVALICPVVDVYPGKQKIIVHGGAIHLSK
                     DRVGIDGADCYGFVCRFDGKGWSNPIPNAMVTSLSQEHGVISYSGNDLDGIKPGDLLA
                     ILPVHSCLAVDCMGQTFDSEGNTYDVLPKNKR"
     gene            53625..54959
                     /locus_tag="PLW22_00235"
     CDS             53625..54959
                     /locus_tag="PLW22_00235"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOU73155.1"
                     /translation="MKRLTQFLLFSVISTLTTAQSTKSNSVFDKYFENKTMRVDYIHG
                     GNSFSEDFKIFGIKNDGEWGGRVRVMDDPYILGLYAFDIIDAASGKVLYTQGFCSVFG
                     EWQTTAEAGKLNKNFEESVRFPWPKDIFKLVMRKRDSTNTFQVIWSETINPSMYADAN
                     HAYPAAKVQYFVKSGNPKEKVDIVVLADGYTVADSAEFRKDVERFTEAFFSVEPFKSR
                     KTDFNVAAVYSTSPTSGIRLQKHNYYPANVLGCTYYTFGTERYVLTEREWTVRDFASA
                     VPYDFLVILLNSNKYGGGGIYNLYITASAKSSSSGYVMVHEMGHHIAGLADEYYTSDV
                     AYQVSAPKYEPWEFNITALLNPASLKWKNMVEQNTPIPTPWQKENYERTGKLRIADEV
                     YSGKVGAFEGAGYLSKGMYRPEVDCIMFSHSLTFCRVCQEGLNRVLDMYVVR"
     gene            complement(54952..57138)
                     /locus_tag="PLW22_00240"
     CDS             complement(54952..57138)
                     /locus_tag="PLW22_00240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU73156.1"
                     /translation="MFKPITFKAFLLLVVFGIPTLRVNAKPEPLDNIDSLKYFENLTR
                     QYERNGDKVKLVETLIAKSQYLFNKKNLDNALIEANQALIVSLDMGREQYLEKAYYLL
                     SQIHIEKGLLYESIDYLYPGYRYSIATRDSLKIGWYLLSISKVEGELGRLNNAIENNL
                     TAINFFKSRNDSLSLAKIYTSQGIIHTEFGNFVTAQSYLENAIAILKVVVDSTYLGIT
                     YRALANNSLRQSNNLMADKHLKKSETYLKNNPKELARTQTVGAELLINQLKPNSAIPL
                     LQSILNTQNEINDRYGQLYPLLLLGKAHARVGNYSESKKYLLQCLKQSESLSITNISR
                     LAYKELSQLEELQGNKTLGYQYLRKYISLTDSLFNNQIISEANRLENISALRQKEKEI
                     QRQRENLLLSQANLNKSRLRQLFLVGFIAILIVLVVVSYLAFRNKQKANLLLAEQKEK
                     IEKQKNMLEQRSRDITDSLNYARRIQKAILQTSEQPTDFFDDSFLIFLPKELVSGDFY
                     WFKRFNNQMLFAVADCTGHGAPGAFMSIIGMFGLNQIVSELNETNPGEVLQNLNELFH
                     KSFEQREGSEIFDGMDIAFCNYNPETRELRYSGANIYLNIVRKSNSQKVSNIILNQNE
                     THTLYQVKCERQSIGYVTEKVSFVTHSIQLEKDDIIYLYTDGFTDQFGGPNGKKFRLV
                     DFRNILCSIASYPMAKQKDFLLEIFSEWKGDNVQVDDVTVMGIRIT"
     gene            complement(57206..59149)
                     /locus_tag="PLW22_00245"
     CDS             complement(57206..59149)
                     /locus_tag="PLW22_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73157.1"
                     /translation="MTKNNKNHIDINDLPIAVLLVKRDGTVLNYSPSVLLLTDKPIPD
                     GCKIESLFPNLPFKTSKKERTYTFEHSTHGKQHTYRANVKPVEWNIFLVWIENITLSN
                     KVERINRILVKLSAIGQETTSPEDFYRLMQEELNTLFDAMNIYLVLFDKSRQRLSLAY
                     VSDTHPINTDYPSGNTFALWVAHRGQGVILNRQQMNRLRSKHNLTFYGPPALCWMAVP
                     LKTKSDTIGVLAVQNYQNPNAYDHNDLAVLEFVSSQLTYMIIQKERENELMLAKKKAE
                     EADKLKSAFLANMSHEIRTPMNAILGFSELITRETISPEKKKIYADYITSNGRLLLTL
                     IDDIIELSKIEAGIYSIRRQPVELDSLFNELHQFAINEKKRQKQNILIARDIGNNSGI
                     THILADGERLKQVLLNLLSNAIKFTSQGSVTMGYRIPNNATIEFFVSDTGIGIPKSLH
                     EKIFERFRQADETAQRLYGGAGLGLAISKRLVELMGGRIWVESEPNKGSRFSFTLPLI
                     IPNTKNISRINFITKNDYTKIKGKTILIVEDNEANVNYLSDVVIASGAKSLTAMSGSQ
                     AIDLLNREKIDAVLLDIQLPDTNGLELISRIKEKIPNVPILVQSAYTPAEYIDKAIAR
                     GAYGYLTKPIKVNELLDLLSELF"
     gene            59345..60466
                     /locus_tag="PLW22_00250"
     CDS             59345..60466
                     /locus_tag="PLW22_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU73158.1"
                     /translation="MHKILYVLLFAIISISCGEGGKPSSNSIHGNGYAKHAKGFDIEI
                     DGSVTKVWVNNPFEPTANGLVYTLYADSMRSIDPNSIQIPVKRVVCMSSTHVGFIGAL
                     GANSAIVGISGTKYVNNPKLWERVVNGQIAEVGYDQTLNYELLTRLKPDVIFVYGVSS
                     ESLSFIDKLKELGLKMVMVSDYLETSPLGRAEWIKFFGVFFGKKELADSIFSEVEVNY
                     AKLTQSVEQVDTKPKVFLNLPFRDIWYFPGVDNYFVKLINDAGGSYIFPELTGSQSHP
                     VSTEVAYKAGLSCDIWLNPGTANSLSDITNSDNRFSMFKSFGNARVYNNNKRMGQGGG
                     NDFWESGVVHPEQILSDMIKIFHPELMPTDTLYFYRRLE"
     gene            60496..60816
                     /locus_tag="PLW22_00255"
     CDS             60496..60816
                     /locus_tag="PLW22_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOU73159.1"
                     /translation="MKFGVIIETKEPEKAWNAFRFAVASLGKGHEVKVFLMGEAVECQ
                     GLTHNKFDVDAQMRKFTAEGGTILACGTCLKSRQMDGTELCPISTMADCIQMVEWADR
                     VITF"
     gene            60834..61856
                     /locus_tag="PLW22_00260"
     CDS             60834..61856
                     /locus_tag="PLW22_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU73160.1"
                     /translation="MQGRTRYTILFISLMVVLLLLALLSLLLGSVAIPLADIKAYFLG
                     GSFTDEVNLSIIQNFRMPKVVSALLAGSALAVSGLQMQTVFRNPLAGPYVLGISSGAS
                     LGVALVLMGSSAFGLSFWGSQVGTVVAALVGSLLVLMLILAVSIRVRDIMTLLILGMM
                     FGTAVSAVTSLLQYFGSETSLKNFVIWTMGSLGGVGRQQLWLYAALVLLGLALAWAYV
                     RPLNVLGIGDTYARSLGFNVKQTRFLIFLSTSLLAGAATAFNGPIAFVGIAVPHLARM
                     VFRTSNHAILVPASALAGAATLLLCDIIAQLPGSATVLPLNSVASLMGIPVVIYIVLK
                     NKRVVS"
     gene            61856..62875
                     /locus_tag="PLW22_00265"
     CDS             61856..62875
                     /locus_tag="PLW22_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU73161.1"
                     /translation="MNGIVIDIKGLVSGYRNGVSSTYRYPAFNAVALRGELIALIGRN
                     GVGKSTLLRTLARIQPSLDGNVFVKRVNFQQISGSEFAQLVSFIPSEPAHASHTTVYE
                     FVSMARYPYHGWFEALNDNDHKLVDDAISAVGMQNFRDRFIDNLSDGERQRVMIAFAI
                     AQDTPIIMMDEPTAFLDLPNKFEVMRLLREQAQKGKTIIISTHDLQTAFGLVDTIWLM
                     LSDGVRVGAPEDIVLSGNLNRLMDDTPVLFDLKSGQFVYKHQTRLVACVLGGSPNIKK
                     WTSHALRRMGYEVEFSPIDDASTYVQIQEHEGKVQWILGRDDRNPTFDSIRNLCIYIK
                     GTALL"
     gene            complement(62870..63640)
                     /locus_tag="PLW22_00270"
     CDS             complement(62870..63640)
                     /locus_tag="PLW22_00270"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU73162.1"
                     /translation="MKIAVIQSKLYWELPDNNFEQFEQKLAGIEPGTHLVLLPEMFTT
                     GFSMSPASLAERHPGRTVEWMKSMAKKHGFALAGSYIAETDGKYFNRMVFAYPNGTIE
                     TYDKRHLFRMANEDAHYTSGTQRVIVTHEGWRILLAVCYDLRFPVWLRNWNDYDLMIL
                     VANFPERRRYAWNSLLVARAIENQVYVAACNRVGTDGNQINHTGDSQIIDPMGQIIAQ
                     AKPNTEEVIYSSIDKDYLHSVRSSFPVHLDADSFEIKL"
     gene            complement(63615..64469)
                     /locus_tag="PLW22_00275"
     CDS             complement(63615..64469)
                     /locus_tag="PLW22_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOU73163.1"
                     /translation="MNFPPDNQQSFFFYNGKILPLNKFSVALTEGENIIYEVIRVKNS
                     TPIFLNDHLNRFMYSAQMMGYNINLDEITLGIRELLKANPVDQRNFRLMYYSNENGAT
                     LLIYFIPSRYPTADEISTGVRLETLNAERHNPTVKFENKAIRAIANEILETHRCYEVL
                     LVDHDGFITEGSRSNVFFIKDNELITAPNSKVLGGITRQKVIEICNTLQININFRSLG
                     ANEIKQTTGCFITGTSPSVLPVKQIDNHIYHEIPDLVRDISAHYETLVEQCIINWRTE
                     NEDSSNSK"
     gene            complement(64553..65701)
                     /locus_tag="PLW22_00280"
     CDS             complement(64553..65701)
                     /locus_tag="PLW22_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73164.1"
                     /translation="MKKIATYLGIAIACIIGIVIALHLAWYFQKSKPLNIYILDKTVT
                     QTDRIEHKSLVWLLNHFRIVGPDGKSYNHKSDYWGFFPIDLNQQLFDIKSIRLNEVDA
                     YAAVYDAAYYTDCYGVYSYEWYKTETKAPNTSKVYGGLNQNDYLLLKRMKELNKLIIG
                     EYNMFSSPTNALIRTKTEELFNVSWTGWAGKYCPNLGAINNGKGPAEWMVKLYEKQHQ
                     KPWPSKGSGIILISNDGLIDVLEEGSDLLNPRVKLIAEPTMPQWQNNSKETEFYGWFD
                     IIMAGIGSEVHATFKLETTPAGEEKLKSFGLTNQFPAVVKGYSDKFCYYFAGDFAENS
                     VSIATSKLKGGKTLNSILHLNSPRNAFFNNFYLPLINNILDIYTSREQ"
     gene            65750..65893
                     /locus_tag="PLW22_00285"
     CDS             65750..65893
                     /locus_tag="PLW22_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73165.1"
                     /translation="MKKITSEGDKFVKRESLTVNREPLKVKREKLKVKSLALSGNESE
                     YSE"
     gene            complement(66407..66715)
                     /locus_tag="PLW22_00290"
     CDS             complement(66407..66715)
                     /locus_tag="PLW22_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU73166.1"
                     /translation="MIKPETLQSAPKVPFNLDGRIMFSSSKVEMVHLTLKPGEVIPAH
                     ANPFGVVFYLLEGELELTVNNESALLTPNSATFVEINESRSLTNKSTNTARVLVVKIF
                     "
     gene            complement(66728..67513)
                     /gene="ccsA"
                     /locus_tag="PLW22_00295"
     CDS             complement(66728..67513)
                     /gene="ccsA"
                     /locus_tag="PLW22_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOU73167.1"
                     /translation="MVWNYFVPVALTVILLWLVSVYFNHKESAKGKLILWYSGVTASA
                     LLGVFIILYWFDIKRPPMRTMAETRIWYSFLVSLVSLSVFKAYRSKNILTIGLIMASV
                     FLVVDIIHPEYQGRFLMPALQSPWFIPHVVSYMLAYAVLASASLSVAWTSFSKGSVHD
                     ELQQQMRLVYPGFALLTLGMLLGAYWAKFAWGDYWAWDPKEVWALITWFFYLLVIHIH
                     RYMPTKHKLLARLMIVALWVLLITWLGIKYLPSASQSIHVYGG"
     gene            complement(67518..68735)
                     /locus_tag="PLW22_00300"
     CDS             complement(67518..68735)
                     /locus_tag="PLW22_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73168.1"
                     /translation="MGNQFKKKPLWLWPWGFLEGFIVSVGLWVLAFFLELFVPLSLFP
                     KLGFPTNAIVLVTILSLLILLKIIFPKSKFFNWLASVKSAIPSISLFLFSIILMGIIP
                     QIGHGMGIPGALFNLLGSWLFLFPLIFMLITLGAAVVNRVFPITLKNIIFTLNHAGLW
                     VCLSAGLVGSSDKIEATVQVNQNEIAWYGTDKNGSVIELPIAIELQKFEAQFYQPRLA
                     LMGSKTFFPESEYDLNSNPSIKIDNLTIQVMQYLPKAFATDSTFIDARGVPFTGPAAM
                     IQVFDSNGQTVTMGWISSLTHVTAEKTLPLPDGRELRLLPAEAKYFGSYAKIYSKKSS
                     SVNQCLIEVNKPFRVDGWWIYQYSYDNLAGPDSTYSSFKVIYDPWLPLIYIGFAMMLL
                     GGLGLVLINDSKK"
     gene            complement(68747..70258)
                     /gene="nrfA"
                     /locus_tag="PLW22_00305"
     CDS             complement(68747..70258)
                     /gene="nrfA"
                     /locus_tag="PLW22_00305"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HOU73169.1"
                     /translation="MKTPLSEKIAKNPWIGWALFLGTMIVVFVLGLLAASIVQRRTEA
                     NLMNTPRYKIDEFEPRNEAWGMAFPREYQTYSQMSDTLFRSKYGGSAFRDLLDEDPRL
                     VILFAGYAFSKDFNQARGHVYSVADVHNTLRTGTPANENEGPQNSSCWACKSPDVARV
                     MAKMSPADFYKTKWSAMLSEIVNPIGCANCHNPSDMTLRLFQIPLKEAYERMGSNVEL
                     LPLNKMRTMVCAQCHVEYYFKGDGKYVTFPWDKGLNVDDIEKYYDEYAFADWIHPLSK
                     TPIIKAQHPDFELFQHSIHYQRGVSCADCHMPYVTEGSQKITDHKVQSPLNNMEASCM
                     VCHKQTKEELIKNVYDRQDRVYTQKIVLEDLLVKAHIEAKFAWDNGANEKMMEPVLKL
                     IRQAQWRWDFVAASHGASFHAPLESMRIIADGIEKASEARLQLSRVLATLGHNEPVPM
                     PDLSSKEKAQAYVGIDLKKLKGEKEEWKKNVLPKWLQLAKEREQNMPLPERIM"
     gene            complement(70278..70865)
                     /gene="nrfH"
                     /locus_tag="PLW22_00310"
     CDS             complement(70278..70865)
                     /gene="nrfH"
                     /locus_tag="PLW22_00310"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOU73170.1"
                     /translation="MFDWAKQILKRFQPPPNWRVPVIILAGIFTGTGLFVFYTSRAWS
                     YVSDDPATCVNCHIMRTQYVTWHHSSHREHATCNDCHVPHDNIVRKYYFKANDGLRHA
                     TIFTLNTYPQTIYMHKPGQRVVQENCIRCHGNLTAMVKANVDLDAILAGNGKRCWDCH
                     REVPHGTVKSLSATPYGTAPVPKTDTPNWLKKLIK"
     gene            complement(70906..72411)
                     /locus_tag="PLW22_00315"
     CDS             complement(70906..72411)
                     /locus_tag="PLW22_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HOU73171.1"
                     /translation="MSFYSTLRKYHKWPALVFTLFFLLFAISGVLMNHRQTIAHIDIN
                     RKYLPKNYQLKNWNLASVKGARTINSDSIILYGGVGIWLTKSNFENWKPLMDGIPEGS
                     DYRKIFDLEVTPNGILAATRFGLYWLGKGENHWVKLHLPQSDEFCTSIEVVESSIYIT
                     TRNSIYYSSAQTLQFTPIDLKAPLGSKKEISTFRLFWNIHSGEIFGIPGRLLADLVAL
                     VMIFLSITGIIYFLSPKIIKRYHEQFRFHKLKRTTRFSYKWHLKVGALATLLLVISGL
                     TGTFLRPPFLLTIANSQIVLNNQKVSNSYYWNDKLRDIRFDSARKKFILATSDGIYHC
                     TDFSEPLTKFPNQPPVSVMGINVLEPLDSVFYLVGSFSGLFRWNPDTGETFNYITGNL
                     HRRENGLRKPLGENVVSGYAHISGLEYIFDYDKGMVALHHSEPAIPMPSTVADAFKFP
                     LWNLAQEIHTGRIFSFMLGDFYILVVPLTGIFLVVVVLSGFMMWYKRKYLN"
     gene            complement(72445..72966)
                     /locus_tag="PLW22_00320"
     CDS             complement(72445..72966)
                     /locus_tag="PLW22_00320"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOU73172.1"
                     /translation="MDENKLIITPKTKVLQLIEAYPQLEEVLIGYVPAFEKLKNPVLR
                     KTVARVATLQQAAAIGNVKVEELINVLRKAVGQDLLEGADEMEFTTQKPQWFDSKKVD
                     SEIDVRVMLQAGEQPVNQIMADLTDLSSDKIYKVVAPFITAPLIDKASSLGIKHWVEK
                     IDDEHFNIYFYKP"
     gene            complement(72969..74201)
                     /locus_tag="PLW22_00325"
     CDS             complement(72969..74201)
                     /locus_tag="PLW22_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOU73173.1"
                     /translation="MSELINNSENRKKKLKELILRLHKGDSEQEVRQELIQSLTNIPY
                     GEVVEVEQELISEGLPEQEVLKLCDVHSAVLKGNIDLTTVKKIPDGHPVDVFIKENKE
                     LNQLCQSIEQSLMELVSSEAVDIPKLVLKLRGQFNALFDVDKHYQRKEYLLFPFLEKQ
                     GITGPPKVMWGKHDEIRELIKGSIELLQTEGISRDELIASSEIVLRPAIKGVMEMTTK
                     EEEILFPMALDKLTEADWYEIHKQQLEIGFCLYDPPTKWKPSWVEGSELQELNKTAEN
                     IQLPTGSFSVEELLAILNTLPVDITFVDKNDKVKYFSQSPERIFQRNRAILNRDVRLC
                     HPPASAHIVDKIIEDFKSGKASRAPFWINIGGNLIHIEYFALRNEKGEYLGVLEVSHN
                     VSVYRKLEGEQRILSYSK"
     gene            complement(74263..74586)
                     /locus_tag="PLW22_00330"
     CDS             complement(74263..74586)
                     /locus_tag="PLW22_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU73174.1"
                     /translation="MNEFPKGQKFNFMNEVEYSNGGIVSKNVLKRPTGNISLFAFDKG
                     EGLSEHTAPFDAVVQVIEGKATIIIGGTPHQLVAGETIIMPANIPHALQAEERFKMVL
                     TMIKE"
     gene            complement(74670..>76292)
                     /gene="hcp"
                     /locus_tag="PLW22_00335"
                     /gene_synonym="priS"
     CDS             complement(74670..>76292)
                     /gene="hcp"
                     /locus_tag="PLW22_00335"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOU73175.1"
                     /translation="QEAAKGVGCTIKGVCGKNDDLSNMMDLLLFVTKGICVYSTALRE
                     QKGYESPMVNKYVFDSLFSTITNANFDKAAITKRVEKGIEIRNMLREEAQKKGVKVDT
                     TFEGCTWEGKPEEFDAKAATVGVLNETNEDIRSLKELITYGVKGMAAYAEHAYNLGFE
                     DTTVYAFMQKAMADITKNLNADELVALTLETGKYGVQTMALLDKANTTNFGNPEITKV
                     NLGVRNNPGILISGHDLKDMEELLAQTEGTGVDVYTHSEMLPANYYPAFKKYKHFVGN
                     YGSSWWHQREDFESFNGPILFTTNCIVPPLANASYKDRIYTTGAAGLEGAKHIPDREN
                     GKPKDFSSIIEHAKRCKPPVEIEKGEIIGGFAHEQVFALADKVVDAVKSGAIRKFIVM
                     AGCDGRMKDRNYYTEFAEKLPKDTVILTAGCAKYRYNKLPLGDIGGIPRVLDAGQCND
                     SYSLAVIALKLKEVFQLNDINELPIAYNIAWYEQKAVIVLLALLYLGVKNIHLGPTLP
                     AFLSPNVAKVLVENFGIGGITTVDEDIKLMVG"
     assembly_gap    76293..76392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<76393..77294)
                     /locus_tag="PLW22_00340"
     CDS             complement(<76393..77294)
                     /locus_tag="PLW22_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU73176.1"
                     /translation="MKRDIITIDRDKCNGCGLCVSGCHEGALQLIDGKAVLISELMCD
                     GLGACIGECPEGAITIEHREAEPYDEVHTISKMVANGKNTIIAHLKHLKDYNEKEYLR
                     QGVEWLLANRDKLTFSVDEVLQAVHNHTKDACGCGTSEPKVKPQPMHHHGGGCPGSAT
                     RSFAQAIAGAEGMVSGKSELTHWPVQLHLINPHSGHFRGSNLLLAADCVAFSLGNFHS
                     QHLKGKTLAIACPKLDSNKESYVEKITALIDDAQVDTITVMKMEVPCCGGLVQLARMA
                     MEQAKRKVPIKVITVGIQGEILES"
     gene            complement(77660..78868)
                     /locus_tag="PLW22_00345"
     CDS             complement(77660..78868)
                     /locus_tag="PLW22_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73177.1"
                     /translation="MKKYIRREMYEAKIRPFIGKDLIKVIVGQRRTGKSYFLFQVMDL
                     VQQLNPDIQILYINKEDLAFDSIKTYADLVSFAESNVTKGKKAAIFIDEIQDIEDFEK
                     ALRHFHTSGMWDIYCTGSNAKLLSGELSTYLTGRYVEITMYSLSYPEFLVFNRLENTP
                     ENFNKYLRFGGLPYLVNLTLNENVVFEYLRNIYTTILYKDIITRYNIRNAAFLERLVI
                     FIADNIGNVFSAKKITDYLKSQNIKSSNNIVLDYLNYLQNAYLIAKISRNDLKGKRIL
                     EIGEKYYFHDTGLRHAIISYKPGDINQLLENIVLLHMLIHGYSVTIGKLGDKEIDFIC
                     DKNSNRIYIQVTLSLADENVRTREYGNLLETGDNFRKIVVTADEYALDTYKGIETINI
                     REFLTGTKIP"
     gene            complement(79584..80996)
                     /locus_tag="PLW22_00350"
     CDS             complement(79584..80996)
                     /locus_tag="PLW22_00350"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOU73178.1"
                     /translation="MKKFFLITLTLSLMVFASAFAQKNGYRIEVKIDGLTQGDLLLGY
                     HFGDKKYVKDTATVNSKGVAVFEGDTLLPGGIYLVILPEKNYFEVLVDKNQKFSVATS
                     KDNLLENLKFTNSSENEGFIKYQRFMIDMQKQSKELSEQLKESLSDSLKANAIRDKMN
                     ELNNKVLAYWDEVEANYKGTLLAAIIKAMRNVEAPKHNIAKDTPKYDSLVWAHNYNYN
                     RYHFFDNIDFSDARLLRTPILESKINVYFDRVLLPLPDSIIPAAIELIEKAKANKEMF
                     QYILSTLTNKFQTSERMGMDGVFVAVAEKYYLGGQAWWADQKLLDKIAERVKAIKPNI
                     IGNIAPDLWLPNPNGKYYRLSDVNAKVTVAYFWDPDCSHCKKVTPELKKVYEKYKGKG
                     FEVFAVYTQGDQPKWMEYIKANELTWINVWDPTFSSNFRNLYDIYATPVIYVLDKNKK
                     ILAKRISVETLEKILEEELK"
     gene            81126..83138
                     /locus_tag="PLW22_00355"
     CDS             81126..83138
                     /locus_tag="PLW22_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOU73179.1"
                     /translation="MDTWKDARPNRLVREVSPYLLQHAYNPVDWFPWGEEAFAKAKAE
                     NKLLLISIGYSSCHWCHVMEHESFSDEKVAKTMNERYVCVKVDREERPDVDQIYMNAV
                     QIITRRGGWPLNCFALPDGRPVYGGTYFPKENWLSILESLDETWKNEPQRIYEVADEL
                     AEGIANTEIIRVKADVSNVNLNVVLAENLNSFRNQLDFKLGGTLGAPKFPMPGLLKYL
                     LITGQHSQRKDLLAFVDTTLERMANGGIYDHVGGGFFRYSVDEKWFVPHFEKMLYDNA
                     QLIEVYSLAYRLNPNPLYRRVVEETITFVERQLLSSTGGFFSALDADTAGLEGGYYTW
                     GKSEVEQILGYDSELFCVAYGITPTGNWESINILFRSLTNAQLSSLFGLPEGEIDYRL
                     KTSLIKLKNHRDFRVPPLVDDKIITSWNSLMVSALSTAYLTFGYDKYLQLALSSANYL
                     IDNHVNDDWTLSRITCKGRVYGTALLDDYAYTINALINLYQITNNTLWNRYAINLAQK
                     TKDIFFDTNSGMFYYTPASTELITRKMELMDGVMPSASAVMVSNLLALAEHDTSSEYG
                     TIALQMIANVGNHIKQGNVFVHTWALQLLSTILPVAEIKVNGDDLNAAEMLIASKLVY
                     PKIKFLQSTDIPNKYLLCIGNTCQSPTDNSENIVKWTNAISFEYYV"
     gene            83629..85368
                     /locus_tag="PLW22_00360"
     CDS             83629..85368
                     /locus_tag="PLW22_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOU73180.1"
                     /translation="MAYSFDSYRVEVLQRPFVRSDGFTTKQTAKIELFNSKGVLITTL
                     SLGIPEMADVYERIGKMEPVNLDYCYIQGFSTIACKRYLQIDKAQPLPVAQFTACNSF
                     FDSPVSIDLSLSISEKEFVIDESYVVTELFDFHLSNLLGSFRLTNCYIKAAKSNFTQV
                     RFEGEQISFKNTIFDVGVKDFQDTSFGSGEAIFTNVDFGDGDVSFINAGFNNSDVDFR
                     VCRFGTGKVDFHYARFGGGGVSFERVDFGSGRVDFRAAEFRAGRTSFNRASFHEGELN
                     FEGAETPKGKLTFKRAAFGNVTLIFDLYQGAKSEIVFERSLFSQNISFSEAQLGKLIF
                     SDCQFNSMVNLHVKQSDEINLAGCTIRDIVEFYTHGDPPRINSLNLTGVRLLGQIYID
                     WDANGVRSLIYSQGCTTVAEKAEQFRILKENFNALGKYDEEDAAYVEFKRCHMRALLL
                     SSNKQGKLRHLINRIIYWAKKLLFDYMGLFATSPQRVIFSILVVYVIYSLAYIAIMGL
                     GLGHIIATSASGDQLGIVARGFYFSVVTFFTIGYGDFAPSGIARFVAGTEGFMGVFLM
                     AYFTVAFVRKILR"
BASE COUNT        22652 a        17569 c        18924 g        26105 t
ORIGIN      
        1 cggcattggt gtttactgtt gaaaagttct ggatgagctg ggccggaact cgacttgtga
       61 acatatcgcc catgcgagcg taagcccatt gcttgcggct ccagttttca cccatttcgg
      121 ttttttcctt cagggtgtag tatgggaagt ttagggcagt aataaatgca atcttgttgg
      181 catagaggtc gtcgtttaaa tgtgctgccg cgttgtatga gccaaagagt tcatcaatgg
      241 gggttactgg cccccagtcc aggtggaggg gtttcatgag ttccttactc aacaggatga
      301 agtagccatt tagcatttca aaaccatagg atagcttgtt gaatagctgc tccagcgctg
      361 catcatccga tacaaattgt gtggtgcaga actgctcaaa atcggtctca gtgccatcgg
      421 taatcctcca aagcatggcc acttgagcaa ctccgcgttc aattctttcc ttttgggaat
      481 cgccgtgctg tttaaccagt tcagcagtta cttttttcac tgtggcatcg ctaatgggcg
      541 atacgtccac cttttgttca ggttgtttgc actgtgccat aagcaatgcg gttgcaatgg
      601 ttagtagtgt aaatattttt ctcatggtga gttggtttga gtttaacaat gtagttacaa
      661 aaatgcaaat tcataggtta gattgcaatt cattctgaaa gtttatcaag tttttttact
      721 atagaattat ttttgccgat gtttaaaagt gaattagcta tggaatgtaa aaaggagaat
      781 aactccaagt tttgcaactg ctcctacccc tgcggtaagc atggtgtgtg ttgcgattgt
      841 ttagcctatc accgtaagaa tggcgaactg cctgcctgct atttcccagc cgatattgag
      901 cgaggatatg ataggtccat tgacaatttt atcagtgtgg ttaaggaaag gggaatgggt
      961 tacttaaggt agataaagaa aaaggtgaca cgtaggtgcc acctttttgg tgtgtaactt
     1021 tttactattc aaattgttta aaagcttctt tgataaggcc aatagcctca cgtagttgct
     1081 cctcagtaat aacaaggggt ggagcgaaac ggataatatt tccgtgagtt ggctttgcca
     1141 gaacaccttt ttcggccatg gccaagcaaa cgtcccaagc tgttttcccg ggcttattac
     1201 ggataaccac tgcgttaagc aatcccttac cacgaacaag ttcaatcatt tcgctcttga
     1261 tgttcttcat ctcatcgcgg aagattttgc caaggcgttc agcgttttca gcaagtttct
     1321 cttccttaac tacctcaagg gctgcaatgg caacctttcc ggcaagtggg ttcccgccaa
     1381 aggtagagcc atgctcgcct ggcttaatgg taagcataat ctcatcatcg gcaagcacgc
     1441 acgaaacagg cattgcgccg cctgaaatag ctttacccag aattaggata tcgggacgaa
     1501 cacattcgtg gtcgcaggct agcattttac ctgtgcgggc aataccagtt tgaacctcgt
     1561 cggcaatgaa aagcactttg tgcttcttgc agaggtcata agcttttttc aggtaacctt
     1621 catcgggaac aaatacacct gcttcgccct ggattggttc aacaaggaaa ccagcaacat
     1681 tagggtcctg caatgctttt tcaagggctg gaatatcgtt gtagggaatc ttgataaaac
     1741 caggagtgta tggaccgaac ccgccatagg aatctgggtc ggttgacatg gaaattacgg
     1801 tgattgtacg gccgtggaaa ttgttctcgc aaacaataat cttggcctca ccatccttaa
     1861 ttcccttaac gttatatgcc cagcggcggc aaagctttag tgcagtctca tcagcttcgg
     1921 ctccggtgtt catgggtaaa accttgtcgt aaccaaagta tttggttaca tactcctcaa
     1981 attcgccaag tacgttgtta taaaacgcgc gtgatgttag ggttaacttt tgtgcttgct
     2041 cgcacatggc tttaatgatt tttgggtggc agtgcccctg gttaactgct gagtatgccg
     2101 atagaaagtc gtagtaacgc ttcccttcaa catcccataa gtaaacgcct tgaccccttt
     2161 ccagcacaac aggcagtggg tgatagttat gagcgccata cttgctttcg cgctccatgt
     2221 aatcctttgg tgtcatcttg ctcatagctt ttgttttttt gttatatgtt gatatttaaa
     2281 tgattatgtt gcttctaatc tattatttgt cgttttgcaa ggttaagtat tgtaaaccaa
     2341 ttttcaaaga gttgtttaga tttacaaaat atgttaagaa acataagtcg gaaaaccttt
     2401 gaaaacaatg atattcataa caattcgtaa tgattttagc gattgaattc agggtaaaaa
     2461 acctatacgt tgtaatggct ttttaaactt tgttggcgtt atcttgttaa tgcaacaaat
     2521 aattcaaaaa tagaatgaac gaaatattac aatcaattca gcagcgccgg agtgctatgg
     2581 cctttagccc tgagccaatt ccaaatgagc atttaattca gctttttagg gctgccatac
     2641 tagctccatc ggcatataac gaacagccct ggatgttttt tgttgcaaac aggggaaata
     2701 cctcagcctt tgaaagtgtg cttaatacct tggccccagc caaccaggag tgggcaaaga
     2761 gtgcaggtgc attagtggtt actgcagccc gtaaaaacta caccagaaca ggtgagtcca
     2821 actactatgc cctgcacgat ttgggtatgg ctacagccat gtttttagtt caggctcaat
     2881 ccatgggtta tgttacacat atgatgggcg gttttgacca tataagcatt agacgtgcta
     2941 ttaaactcga tgatggctac caaattggag cggtaattgc tttaggaaaa cccggaagca
     3001 tcgaaaatct taaccctcag aataaacaaa gagaattgtc gccacggatt aggctggatc
     3061 ttgaaaaggt cctaagggtt ttatagataa taggttgata acaagtagca taagaggcac
     3121 cggtttgggt gcctcttgaa ttaagttttg gttgaaaaac tactgcaatg ttataccaac
     3181 aacaaagaac acattttctt gtgctggatt aactacaaag gaacctttta taggtaatgt
     3241 aaaattttcg gtaagtttta tgtccttgct agccgaaaat ccaacattta caatgtttgc
     3301 actattgtta taaagtccct tggctggtgt tgctcccaga aaaaattcta atgagtagtc
     3361 gctcaagtta aagggatagc caatttcaaa ataggtggag taatactgat tgccatcagc
     3421 atcacggtcg tttccgtaaa agaatgtggc tgctagcgct ctaagcggaa aattctcagt
     3481 gccgttccat gcgatggttc cctcaagtgc atgacctgtt aattttctat cccaggtaaa
     3541 atattttatg gatgataaat cggtctcatc ctcgttgtag taatcgaaaa cagttaacga
     3601 aagtgggcca aattcataga ttaggtaaaa atcaatttcg ggaaatttgc tgttatagcc
     3661 atatgaggcc caactaccaa aggtaaaacc gccgacgctt aatgataaat cgggttggat
     3721 gcagggattg ttgtcaagta aaagtccgcg ccaaaggtac ctgcttacca tgtcaacctt
     3781 aaaattaagg ttaattttat tttgggttgt ggcaacctcc tgtgctttta tacttagggc
     3841 tgcaattagt attaatattg ataaggttag ttttttcata gagaaattat ttgtgttaaa
     3901 agcaaactta ctttgaataa gtcatgatgt caatatttat cgctttaaat tttattgatt
     3961 tacatcactt ttctattgca tattacatta taactacagt tttcgcaaag cttgatgttt
     4021 gatgtctgtg aagatgggct atcgtctact attttgaata tcaatgattt taggtaacta
     4081 ataaattctt cccaaaactc atcggtatcc ggaacttcgg tgcttggtat tccatcggtg
     4141 ctgcgaacat accaaagcag aatgtcaaca tcacctatgc ctaaactatt ttcttgggta
     4201 taaatgaact tgtaaacaag ttgctggaac agtccatcct tatcggtttt cccatcaaaa
     4261 tcgctgatgt tgctaatttt tgttttggct ggttcgtgtt taccggtttt aaaatctaca
     4321 atggtcagtt tcccattttt gagttccaaa cggtcaattt ttccgccaag tcggacgttt
     4381 aaggctttac cgctaagttc aattggtatt accgtttcaa cttcttcctc aattgccaat
     4441 aatttatatg gtgtcctgtt aaggtcggaa cctagggcat tccttaccat ccagttcacc
     4501 ctttccagca gcaacctatt ccgcccgttc agctcatcga tatttccgtt aaacttacta
     4561 atttctttaa aagccctaaa aacagtttct tcaactaatc caagctggtt aagtctgttc
     4621 tggatctggt cttttgtcac ttctttaccc acaaaaccgc tatagatttg ttccatagct
     4681 ttatggaata cgctaccaat gcccatggca tcgagctctt catcgagctc atcgggggct
     4741 gatatgccta acactctgtc gaaaaagaaa cttaaggagc agttcttaaa ttccgatagt
     4801 gccgatgggg agaggtacct gctaccctcg gttgtttcaa aatacttact taatctttta
     4861 gcaatggcct cattttttgt aacctcaata ggtctttcgc tatttacgcc aagatcaaag
     4921 tttacggtta cttcttttat gtttaggttt gactccattt gaagctgctt aacaaagcga
     4981 ctcagttcac ccgattttaa tccctcggtt atgtttgtgt atacaaggta gatattttcg
     5041 gcgcgttgca ttaagcggta aaagtagtag gcgtataccg cattttggtg cttgtagtcg
     5101 ggcaatccgt atgcttgccg tagggtaggc actatgaacg atgggctaaa gtggcttccc
     5161 ggtaaaagat tgtcgtttac cgaaagaatt acaaggtttt taaagtcgag gttacgggtt
     5221 tccaggaaac ccattatctg tatacccgaa agtggctcgc ctataaacga tactctttgc
     5281 tcagctaaag ctttggttag gtatgagcgg aatgttttgt tggagtattc ttcatttatc
     5341 tccgatatca ctaggcctaa ccgttttaag gctcgattaa tggtaagcag gtattcaact
     5401 tcaaacttaa gcttatcgtc gttagcatca atttcatggg ttaatcggct aataattgag
     5461 ttaatgcacg tggcaaggta gtttgccagc tctgaaccct tggttacagg cttgaaaatt
     5521 tgctccagga tgtcgcttcc actaaaggtg tcggggggga tgtttagcaa ttgcctatcg
     5581 atgattatat cccttatcct attggcctca tcggggcatg caagttgaat gtagggatgc
     5641 gatattatta gcagaacatc gcggtagtaa aacttggttt gtttatcgct cgatttttgg
     5701 agagttagca gggcatcaat aaaagagtag gccgaagttt cgcgcagcgg atactccatg
     5761 gtaatattgg cattactgta tttttcgggt aatgccgtaa gcagcggaag taaaagtttc
     5821 tcgtcgggta aaactactgc cgtggttagc tcattagtgt tgttaatgtt tagctcatca
     5881 agaatatcgg gaacaatttt ggtttgtgtt acttccgatg gcgatgagat aagtgttacc
     5941 tttttgctag agccaatatt cccaatgaaa tcaaaatttg tgttgctaag agcattcgga
     6001 aacagcttaa gattctggcg catgaatagt cctgcctcgt gatcgacacc tgttggggag
     6061 tttatatagt aattgtcgta gtcccaatag aaaagcgctc tttctccact ttttagaagc
     6121 aaaagcaatc gttgttccga cttgcttagt gcattaaacc caataaaaac aaatttttgg
     6181 tttggtatgg ctgatgtgcc gccctccaat gcctttacgg ctagccggta agctaatccc
     6241 tcgtaagcta ttccttccga tattaagctg ttggtaaagg cactgtaaat tccgtaaaga
     6301 tttgcccaaa tttttgagta gcgtgaacga attagcgatt cgccttcaga tgaaacaagg
     6361 tttaggaatt tttcaattaa ttcaatagtt tccttatcaa actcgttgaa ttgctgctca
     6421 atttccttaa ggtcgttgat attggtgaat agctttttag ggtcaaccag gtatttgtca
     6481 atctggtcga aatcggaaag cattacattt ccccagaagt aaaactcatc gtaaggttca
     6541 aggctgttgg tggcctggca ataagccttg taaagcttat agttgagcat tagcggatcg
     6601 gctggtttta taccagcata gctgaacatc aggtcgctaa tggttgttgt ttcaggcaac
     6661 caaataggtt tagcaatttg cttgctaagg tacttgctga agaaaagcga tgcccttcgg
     6721 tttggaaaaa ccagggttaa ttttgaaata tcatttccgt gtgttgagta taaatgctct
     6781 gcaacagtct caagaaatgg tttcattgtt ttggttatag tcggtaaaag taaattgctt
     6841 gctctttagt gctgtcgcca aattgtgtaa aaaagttttc gctcatcaac tcctcttggg
     6901 tgttggggtc aaggtacata actgtaacct taacctttcc agtatcttcg accttaaact
     6961 tagcgtagtt gtaaacaaat ccttttccct ttgggttttg aatcagcttg ttttctgaga
     7021 aaacaacaaa ggcgtgctcg tttccacgta cggccttagc ggcaaagtac tcgtaggtgt
     7081 caatggtcat gcccccaatg gcatcgggcg atttgtcctg taattcattg tcagcaacca
     7141 gggtgcactt ggcgtaatgt attacaaccc ttacattgta gcctgtattc agggctgcca
     7201 taagctcatc gaagtttttt aacgccttgg gctgagcaat tgctgccgta aaggctgcaa
     7261 gggttatggc aattaaaatg cttacacgtt tcatattttg tgtgtttaag ttcttatcgg
     7321 atttagtaac tggtattaaa ataattgtta gacactaaaa tagcttctta tttttaattc
     7381 taaactttgc tctcaaacaa tcgcccttgc caggtatcgc gtttttcgag caccttttca
     7441 aatgcaatca tcagctggtc ggttctcagg ttgccccagg gtggaactgc caggtagcgg
     7501 ggtggcgctt cgccagtaaa gcgttcaata acaaagttag ggtttaaccg ctcaataaaa
     7561 tcggcaagaa agttaaagta ttcgtccatg ctgaacagct tgaaccattc agggttggtt
     7621 gtgtactgct gctccataat tgttcccttg attatttgca gctgatgaaa ttttacggtg
     7681 tgtaagggta actttgaaat aatatcggct tcggcaagca tttgctcctg ggtttcgccg
     7741 ggtaggccaa agatgaagtg ggctcctacg cgtattccac gtttacttgt ttcgttaata
     7801 gcccaaaccg attgttcaaa ggtatggccg cggttaataa gttgcaaggt cctgttgtag
     7861 cagctttcca ctccgtattc aagggctatg tagtatttgc tgttgagctg ctgaaagtag
     7921 tcaagtttag cttcatccac acagtcgggg cgagtgccaa tcactagccc aataatctta
     7981 gggtgcgaaa gcgcttcgct gtaaagcgcc cttaacttct ccaatggggc ataggtgttt
     8041 gagtaggcct ggaagtatgc caggtatcgg tcggccctcc tgtaacgcac tgcatggaac
     8101 tcaatcccct cctcaatttg ctgggtaatc gatttttgtg gtgtgcagta gctggggtta
     8161 aaggcatcgt tgttgcagta ggtgcagcct cccaggccaa cagtgccatc gcggtttggg
     8221 caggtgaagc ctgcatcaat ggttaacttt tgaaccctgc ccccaaactc cctcttaaag
     8281 tagcccgagt aactattgta tcggcgagtg tcgccccagg ggtaaacttt agtcatgttt
     8341 atagcaaata acctgataaa gatagaaaaa ccttgccaaa caaaaaaagg ccgacaatgc
     8401 cggccttcgg tttatgtggt gtgatcatta aatctttgca agttcctggt cgagcagggt
     8461 acggatttcc tccgacgatg gacggggtgc attggggttg ataattttac cattggcacc
     8521 aattaacaca aaggttggga ttcctttaat ctggtaattc ttggcggcat cggaactcca
     8581 ggcaccatcg gcatgtagct gaacgccacc caatttcttt tcggttacca ttttacgcca
     8641 ggcatccatg tcgtcgtcga gcgatatctt cacaaaaact atctttttcc ccttgtagtc
     8701 tttctcaagc ttgtccaggt ggggaatctc gccgcggcat gggccgcacc aggttgccca
     8761 gaagtcaatg taaaccagtt tgccattgaa gtcggaaagt gacactgact tgccgtttat
     8821 atcgttgtag gtaaaagtgg gggcgctgtt gcccggtgca atttttagtt tttcgttgta
     8881 aaccgattga acctcctgtt tgtaaacagg tgtggtacag gcagcaaggt agttgttgca
     8941 aatatcgttc agcttccaga actcaccaaa ctgaatctct tccattaggt actgtttctt
     9001 aacgtaatcc ctgattttag gattggtgaa aatgctgtcg atcatgctga aaatagcagg
     9061 aagacgttct tgtgcgggtt ttgacgatag ttccttgaag ttctgagcct tttgaattct
     9121 gagctgtgcg tatgtttcaa caagcgaagc gtactcgtcg taactcaggt gttcgccgtt
     9181 gttcatgtcc acattgtcaa agaatgagta atcggcgctg tcggggttaa attcttcgcc
     9241 cgataggtag gccgagtatt cggggtattg gctgcgtaag cgtagcatga agtagcgaac
     9301 ccggttggct tccatctcag caaagttttt gcttttggct tttaacgaat cggttttctg
     9361 gaataattcc ttgcttatgg aatcaatctt taacccaaat tcgttgaggt ttagcgagaa
     9421 aaggtcgcgc caaccgcgga aaatattttg gagttcttta ctgcgtgagc gtaagttctg
     9481 gttcagttca ctgttgcttc cactgaaact aatgccatcg gcattattgg catcaacttc
     9541 aaagtagctg ctatcgccgg gttcaaggta aagggttgtt tgtattcggc ccgatctaac
     9601 cttgagcata gtaggattgg ccagggttac cttatgaaca aaaagcccat tttcatttag
     9661 ggctatggta tcaatagcat cggggctaat aacaatcacc ttgttttcgg taccatttgt
     9721 gtatcgacca gcaagcgtgg tcggttttgg ctcctttgag ccacatgcaa ccatagcggg
     9781 tatggttaaa tagattaaat actttcgcat tgttaaactg gttttggtta gtatttagct
     9841 aattgtttgg taaagttatt cataattacc ggatttttga cttaaacctc ctcgtgtcgg
     9901 tttaattgga aataccataa atatcccttt acccttgcat agcccatgct gagtagtaga
     9961 tctttgtatt gctcaacctg tttgcggtgt tttggggttg gtaatccaaa tttaaaatcg
    10021 acaattacag cttggttatc gccaataaga actcggtcgg ggcggtaaat attgccatcg
    10081 gtgtttacta tcgatgcctc gtttttcacc tcccaggtgc tgctgaacca ctgtttgtag
    10141 ggttcactgt ttatcatcga tattaagctg ttgtggacat tttgcctttg gtttgcagta
    10201 atgtacccat tctgaacagc ccaatccatg gcctcatcca aatcgtcaag ggtgatnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttga gctgtttggc aaaaacttca
    10381 caaatttgtt ttgttctaca ttttcctggg ttttgttgat ttcgtcttca gcaagctttt
    10441 tatcggtaac caaaatgtat agctcatgct tagcacgggt aaaagccacg tattgcaggt
    10501 ttatatcgtc tatctctttg cagaattgtt catcgtagta atcggcttca aagtatgttt
    10561 gagccaacgc cttttttggt tttattggta aaagcggata atttttaagg atttcgttag
    10621 gtgtatcggc cggaagcttg aaccacatta tgctgtcttg ttgcttctgc tggttctgca
    10681 ggtcggcaaa ggggaatatg attataggga actctaatcc ctttgattta tggatggata
    10741 ggatgttgat ggcattttcg gtttcggcca taaaaagatg tttgcctttt ccatcctgct
    10801 cataccactc aataaatcga cttagcgaag aagtacccct gtacgaaaaa tcaatagcat
    10861 actcatgcag tagcgatagg tagggtaggt ggtcattaat atcgatatct tggtcatgga
    10921 agtacttttg aattattgct tcaaccatat aacccaaagg gtagaatcgg atagagctca
    10981 gccaatcaaa ggtatcgtca ctatcgggaa ggaagacact tggccagtgg atgcttgcgt
    11041 gctgattttt aagcgatgca aattccttac ggaactgagc cacggcaagg gtgtcctttg
    11101 ggtttagggc gattttaagt gcagcaacgc acagccttac tgccgccgat gagttcagta
    11161 ttagcgcatc ctgcgaaact attttgataa accccgatag ctgggtgtcg ctcttgctta
    11221 gctgaagcag gagttcggcc agctgtcgtc cttgctcgtt tttacgaacc aaaaaggcaa
    11281 tatcgcctgc cctgtatccc ctgtctttca aatcaacaag tatatttcta atgtgatttg
    11341 taaagtaatc gttggcttca tcggtctttg gtggcataaa agctacctct acatagcctt
    11401 ccggctcaag tttctttacc tcctgcttaa cgtcggaata gaggtttttt aggttgaagg
    11461 ctgggttttg tggattgata gtttcccatt cgttgatttc gattgctttt tgaaagaaat
    11521 ggttgttgaa ttcaacaatc gttcttgcgc tccggtagtt aaccgttagc tgtttaacct
    11581 gtgggtaaaa ctgtcgctcg gcctcaattc cggccataat acgccaatcg ctgttgcgcc
    11641 aacggtaaat tgattgttta acatcgccta ccaccatgct aagtccgctg cttcctaagc
    11701 tgtcggcaat gagtggttca aagttgcgcc actgcagctg cgatgtgtcc tgaaactcat
    11761 cgagcatgta actggtgtat ttagttccaa tcttttcgta gatgaaaggg gtgtccgttt
    11821 gcgaaacaaa ctccttgagc aacgcgctac tgtccgatag gagcattacg ccatcgtcgt
    11881 taagcttttc cctgagcttt ttgtaaagct cgttgatgat gtaaagcgtt gaaatgttta
    11941 catttattgc cagggcagta ttgtataacc tgtagttgga gttaaggtag ctatagagtt
    12001 cacggtattt tggcatcaga tgttgctcaa caaagccctt aagcatactt attttttcct
    12061 tggtcagcca aaccgatata tcggaaagcg catcttccgc aatgctttta tactctatgg
    12121 gtgtaagtcg ttttagctcc gctagctttg ctatcactgg tataattccg ccttttttct
    12181 tgaaaaaatc gctgtagttg tcttggttaa taccatgtat gccagagctg tagatttgtt
    12241 ccagcaaact tgctgctcgt gtttttagct cattgtatgt ttcggagata attcggttta
    12301 cctctgcttt aagtgcattg agcgtttcct tgttgtgcat tagttcccgc tcttcggagc
    12361 taagtagctt gtagcgctca tggaatagca tgttgcccag gttgatgagg tcgttttgaa
    12421 tgttgggcga tttgttctcg tcaagcctct cctcaatcaa ccttttcaaa tggttaaata
    12481 gttcggggtg tttggtaagc tcatcgagca gcgcatcgga agccttctca atatagggct
    12541 tgtgttccag aaccagttcg ttgttcccgt gttggcctaa ctcccagagc aggttctgga
    12601 ttatgcgttg cacaaagctg tcgatggtgc tgatgctaaa cattgagtaa tcgtgtagaa
    12661 tgttggaaag ggctacttcc gcccggcttc ttattgaatc ttcgtcgatg cctgtttcgg
    12721 ctttaagggt ttgcagcaaa tcgggctctt cactcctttt cagcttgtaa agtttttcaa
    12781 taatacgacc tttcatttcg gcagtggcct tgttggtgaa ggtaaccgca agtatttcgc
    12841 ggtaagcctg cggattttca atgagcattt tgatgtattc ggctacaagt cgatatgttt
    12901 ttcccgaacc cgccgatgct ttataaacca ataaacctgc catatgtgaa aagaaaattg
    12961 aatttggaat ttagaagtta cagaaaatta ttcatcaatt ggttttttaa tatcctctaa
    13021 ctccctcata ttctttgttc cttggggtaa tggttaacga atctctttta gctgttcact
    13081 ttttctaatc cttttcaaat ataaaaaaaa gccgcccaaa aataggcggc ctgtcagaaa
    13141 aatactcgga aattatcgga aaaccagtat gtcgtcgcca aagtagtcaa cggttatggt
    13201 ttgttccttg tttatagtcc cagccagcag ctgtttggag agctcgttaa ggattttctt
    13261 ttgcattacc cgcttaatgg ggcgtgctcc gtaaacaggg tcaaatccta tattggttat
    13321 ccattccact gcgctctcac taatttcaat gcttacgccg ttttgagcaa gcatctgctt
    13381 gattcgttcg aactgcaaca gaacaatctg ttttatttcc tcacggttaa gcggtgtgaa
    13441 cattattatc tcgtccacac ggttcaggaa ttcgggcctg atgctctgct tgagcagctc
    13501 aaatacctgg ttgcgggttc gctcaaatac ttgttcagca ttgttcttgc taagctgagc
    13561 gtagttctcc tggataatat gcgaaccaat gttcgatgtc ataatgatga tagtgtttct
    13621 gaaatcgacc gtgcggccct tgttatcggt aagcctacca tcgtcgagta cctgaagcag
    13681 gatgttgaac acatcggggt gggctttctc aatttcatcg agcagaacca ccgagtaggg
    13741 tttatgcctt accgcttcag tgagctggcc accctcttcg tagccaacat accctggggg
    13801 tgcgccaacc agacgcgata ccgagtggcg ctcctggtac tcgctcatat ctatgcgggt
    13861 catcaggttc tcatcgttga aaaggaactc ggccagagcc ttggcaagtt cggttttacc
    13921 aacaccagta gtgccaagga atatgaatga cccaattggg cgtttggcat cctgtaagcc
    13981 ggcgcggcta cgtcgaacgg catcggcaac agcggcaatg gcttcgtcct gcccaaccac
    14041 gcgcttatgc aacttctctt caaggtgaag cagcttttcc ctttcgctct ggagcatgcg
    14101 gctaaccgga ataccagtcc agcgcgatac tacctcggct atatcctcag catcaacctc
    14161 ctccttgatt agggcaaagt cggcctgaac cttcttgagt tcccccttta ggtgttcaat
    14221 ctcggcctcg gcctgtttga ttcgcccgta acgtatttcg gcaaccttgc cataatcgcc
    14281 attacgttca gcatcctcag cctcatactt catctgttca atactttgct tgagctgctg
    14341 aatttggtca ataacgttct tttcgctttc ccacttagcg cgcaaacggg tacgttcctc
    14401 gtttagctca gccagggttt tggatagttc ctcgagctta ggtttatcgc cctcacgctt
    14461 aatggcctcg cgctcaattt caagctgctt gattttacgc tcaagctcgt caatctcctc
    14521 gggaaccgag ttcatctcaa gccttagctt cgatgcggcc tcatcaataa ggtcaatggc
    14581 cttatcggga aggaaacggt tagtgatgta cctgtgtgag agttccacag cagcaataat
    14641 ggcctcatcc tttatgcgca ccttatggtg gttctcgtat cgttccttta gcccgcgcag
    14701 tatggaaatg gcatcggcgg gttggggctc atcaaccaca acgctctgga aacggcgctc
    14761 aagcgccttg tccttttcaa agtatttctg gtactcgttg aatgtggttg caccaatggc
    14821 gcgtagctcg cctcgtgcaa gggcaggttt taggatgttg gctgcgtcca tagctccctc
    14881 actctttcca gcacccacaa gggtatgtat ttcgtcgatg aataggataa cctcgccctc
    14941 cgatgcaatt acctccttaa ccaccgattt caatcgctcc tcaaactcgc ccttatactt
    15001 ggctccggca atgagtgcac ccatgtcgag cgagaatatc tgtttggttt taaggttttc
    15061 aggcacatcg ccgttaacaa ttcggtgggc aatgccctcc gcaattgcgg ttttacccac
    15121 gccgggttcc ccaataagta tggggttgtt cttggttcgg cgcgatagga tttgaagtac
    15181 acgtcgtatc tcctcgtcgc gaccaattac ggggtcgagc ttgccagtcc gtgccatctc
    15241 gtttaggtta atggcaaagc ggttaagcgc attgtaggta tcctcagcgg tttgcgagtc
    15301 aaccttagcc ccttttcgaa gttcggcaat ggcttgtttc aattcctttt ccgtaatacc
    15361 agcatccttc atcatttggc caacctgctc accggcagca acaagaccca aaaggatatg
    15421 ttcaaccgac acaaactgat cgcccattgc cttgctgaat tcaagggctt tttggagtac
    15481 cctgttagct gtgttgctaa ggtattgctc accacccgat accttgggca tggaatcgag
    15541 catgcggttt aaaaccgcat cgaaagtgcc cggattaata cctaactttc gaagcaaaaa
    15601 tccggtaata ctttctccct cgtccagaat agccctgagc aaatgcgcag gctctacagc
    15661 ctgatggcta taaccctgag caatctctac cgctttctgg attacctcct gcgatttaat
    15721 tgtaaaatta tttagattca taccaaatct cctttctttt cttttcattg aatcgaaaga
    15781 agagcatcaa aaggtttgcc gttgattaaa atacgtcaat atgaccgaat ataattggtt
    15841 ttaaatgcca aaatgacgtt aagcattatc ttttacactg gtggcggttg tttcgtaagg
    15901 gtaagttttg taggaatatt atatgctccg cattttaccg tttggaatgg aacagtttca
    15961 gtagcaattt tttatctaag tgatagtata aacccttgat ggtaaaaaat agcaacggtc
    16021 tttgcatagg aaaagtgcgg gaattttgcc cagcgttcca atcagccgtt ataaaaatta
    16081 aagatattaa aaatgtcaaa aaaccactga accccgcatt ttttctgatg taatgttgtg
    16141 cttttgtgct attttattct tttcttccat gatgtttttg ccgtggcaca tactttattg
    16201 ttcttttgag tcttaatggt atggataaaa gaattatcac ttatatccct ttcagtaaaa
    16261 gacaaaattg tgtcaccata ttgagcctcc gcaataaacc gtccatcaat agaatgtaaa
    16321 taataattat caataaaata atctggaatc gactctatta accattgcaa atatctaata
    16381 ttgtttacat gctggttcga gtcaacatca aaccgattga ctttaaattc cttaaaatgt
    16441 attccagagt cagttggttt aatcttttta gtaatgtcat gatttatact ttcatcatta
    16501 cagtaagacc atctttcttt aatagaatca aaaatttgaa taggtcttct cctcataata
    16561 tcaaagaaaa cccaaagacc tttagctttc cctataatat tccgtttttc atcataaata
    16621 atattttccc taattccttt aatcatcgaa taactagaca tccatgttct aatgatgatt
    16681 ttttctttat aacttggata gcgatcaatt gtcataattc cagatagtaa tacccatcca
    16741 atattttgct tttcgaggtc atataaacta tgatttattg aatagcaatg atcagcagct
    16801 gtttcttcaa gtaaagttaa aattgtagtt gatgcagctt ctccgaactt attcatttcg
    16861 aaatatctta gttcaaattg tttttcaaaa taattttcca aaagctatct ttttaaaatg
    16921 attgtttaag tgttcaattt atctctgagc atgacgcaca acggtttgcg gcttggcgta
    16981 gtggcggatt tttagcacaa aagttcaatc gaagaactgc acttgaacct accacaaaac
    17041 tgtcatacga agcactgcac ccgccattac gccaaaccgc tgttggcggt agttcttctt
    17101 ctcattcgtt ccagtgtatt gctaattgtt caggtcgagt ttgcacttta agtccgtgat
    17161 tatgtgtttc aaagttgttt atgtcgaagt tgcaaaccaa tgcttctaca actgttcgtc
    17221 tgttctcaat tttgccaccg ttatgataaa aatggtcttt tgtaacaatg ttgaacttgc
    17281 gccaaaactt gcttatcatt ttgttctctc tccagtcgtt gtgatgccaa gttgaaagaa
    17341 taaattttgc ttttgtttcg gaaagtaagt taaataaaag ttcttcgtcc tgctctgtcc
    17401 agccgttata gtagtcaacg tgtctgccgt aatacggtgg gtcgcaatag ataatatcgt
    17461 tttctgtcgc aagtggaata atgtctgcaa atgatttatt gtaaaaagtc caatctggtt
    17521 ctggctgtat gatttttgaa atggctgcaa cctgatttgt tattttagta atatatgctt
    17581 gtgaaaaacg gtcaggtttt ttacaaaaag gaatgttcca gttaccttta ctcccaaaac
    17641 gcatcattcc gttgaaacct gctcttgaaa ggaaaataaa atcgtaaggc gaaaactctc
    17701 cactattgaa acgtgtccga actttcaaat aatgttcgta gccattattc tctgctttgc
    17761 tcaataactc gccttctttt tcaaggtatt gtttcattaa aggtgcagtt attgttttgt
    17821 cttggatgcc tttgtaaaaa tttataatgt gtggattgat gtcgttgaga atagcttttt
    17881 tgtagcccga attaaaagca acaacacctg ttccaagaaa tggctctatc cacttgcccg
    17941 aaacttttgg ggcaagatcc ataatccaag gaactaactt ggtttttatg ccttgacttt
    18001 ttattggtgg aacgattact ctcatttcgt tttcttgtta gaagttttag aaacgatttt
    18061 atcccaaagg tcagttctgc ctttaaactc caaaaaatct ttaagtgtgg ttattttggt
    18121 cggctttccg tcttttacta ttgtggctga tccgtaattt atccaatatt cgtcaaacca
    18181 ttcctcacct aatttgctga aaattccgtt ttcgtttttc aaatcttcaa tatctaaaac
    18241 tgaaccaatg tttgcagtat ttccagaacc ttgcctatca cttgcaattt tccatttctc
    18301 tgctgcgaaa aattcaaagt ccttaattac agaggtaatg gattttaagt tcttaactgt
    18361 cgtaactgaa cgctcgccaa cctttttgtt tggtgtttca tattcttctt gtagttcctg
    18421 aacttgataa atctcagttt cagcaaggtc gtctgaaaca tctgtccgag tgtaaataac
    18481 acccaagcaa taatgccctg tgtattgatt gtaggggaac tgaatgttct tatccttatc
    18541 tctctcttta aaataaccac cgtgactgcc aagcgtaaaa cctgctgttt tatcgtttcg
    18601 tctgaaagta gtttttatgt caagtgcaaa ttttacttct tcattgtcct ttttaacaaa
    18661 agtcaagtca ggataccaat tttgtttttc ggcaagaatt atattgtagc cgatgctgtc
    18721 ggcaaactgc aagatttgcg ggaagatatg tatctcaaga atttttgata ctattttggt
    18781 gtcagaagaa atcgtgtaga tgttcttgta aacatcaata aaacccttta cagtccagtc
    18841 gccattgtcg gtggaaacgt atttttcgag cttgtcagca aacttgtcaa gctccttttt
    18901 gaagtccgat ttatacttgt ttttatcctt taaattcatc tctcaaatgt aattaaaatt
    18961 gtcgtctgtt ttgcagtcct ttcaatgaag caccgtcatt ttagaattac cgccaactta
    19021 cttataacca tgctaaaagt tagtttatac agccattttt gggtgtataa ccgtaccttt
    19081 tgggttcggt tttatattca aaaatagtta aaatttttca caaatcatca aacggacttt
    19141 ttaatttttt gtttactatt ttacctaata cttgaatata tttgttaatg attgaatttg
    19201 agattgatag aacgcagatt acgctgattt aattgattta cacaggataa attaaccaaa
    19261 caccaaacac taaactcaat ctcccctgca cactgcacac tgtacactgt acactgcaca
    19321 ctatatttcc attacccgca acagctgcaa cccttttgcc ccttactctc ctgctcctgt
    19381 aagggtgggc attttacgct gccgtatgag cagaatacac agcagtcgcc aggcttgggt
    19441 tttagcagct tcccgcagtt ggtgcattcg taaaagtact ggcaggtgtc ggtaggcatg
    19501 gtttcaacct tgctatatcc acaatacggg catgtgatgg ttgattgcag gattatattc
    19561 atactaacat agaattattt tttgaacgta atgttctcaa ttttgttcaa tatttatagg
    19621 tttggttaga taccaataca atattgctta aacccacagt tgcaaacctt aaccgttagg
    19681 gctatctttg aaaataaaaa ttttatagca tgaagtggtt taaccgttta tttatcagca
    19741 ttttactgct tgttgcagca tttacggctt tcgcacagcg gcaggtaaca attaaagtgc
    19801 ttcaaaccag cgatgtgcat ggtactcttt tcccatacga tttcattaga aatactccaa
    19861 ccgatggtag tttggcgcgt gtttacacat acgtgaagca ggagcgcaac aatcccaacc
    19921 agcaggtaat tctgctcgat aatggcgata ttcttcaggg tcaacccact gtttactact
    19981 ccaactttat cgatagtaca aatccgaata ttgtttcgca gatgttgaac tacatgggtt
    20041 acgatgccgc tacggttgga aaccacgata ttgagactgg ccctggggtt taccgaaagg
    20101 tggaacggga atcgaatttc ccttggctgg cggccaatgc ggttagcaca accaccggca
    20161 agcccacctt taagccatac gtggtgttgg aacgcaatgg tattaggatt gcagtgcttg
    20221 gcctcataac gccgggcatc cccaagtggc tcccaaaaat tctttggccc aacatgcagt
    20281 tcaacgatat ggttgagtcg gccaggtatt gggtgaaata cattcagaaa aaggagaagc
    20341 cacaggtaat cattggcttg ttccattccg ggcacgacac ttcgtatggt ggagccgatg
    20401 cagccgagag ccataacgag aactcatcgc agctagttgc gcagcaggta cccggatttg
    20461 acatggtgct cattgggcac gatcacgacc gggttgcccg taaggttatt aacgcaaatg
    20521 gcgactcggt gcttatactt gaccccggcg cctcggcacg cttactctct gaggctacca
    20581 ttaccctgaa cctgaataag cggggtaggg ttgagtcaaa atccgttacc ggtaggttgg
    20641 ttgagatgaa aaactatgaa cccgaccctg attttatgaa ccagttctca gatttttaca
    20701 agcgggtgaa cgactttgtg agcaggccca ttggaacaat tacagagact atatcgagcg
    20761 caaatgccta ttttgggcca accgcattca tggacttgat tcacgatgtt cagctcaaca
    20821 tttccggtgc cgatatatcg tttgccgcac cactctcatt cgtttcaaaa gtcgactcgg
    20881 gtacaattac cgttagcgat atgtttaagc tgtaccgttt tgagaatttc ctttacacca
    20941 tggagctaac cggtaaggag attgatggat tcctggagca cgcggcatcg ctttggttta
    21001 ataccatgac aggtgctgat gacaatttgc tacgattcaa ggaagagaat ggcaggaaac
    21061 gtctatatgc caactactac aacttcgatt ctgcagcggg tatctactac accatcgacg
    21121 ttagtaagcc tgctggtgat agggttacca ttactggaat ggccaatggc ttgccctttg
    21181 atgagaataa aacctaccgg gtggccataa actcgtaccg agggaatggt ggaggtggcc
    21241 atttaactga gggcgctggc atacctcaca ataagcttca acagcgtgtt gcgaaatcaa
    21301 ccgacaagga tttaaggctt tacatgatga agtatattga gggtcaaggc aaaattagcc
    21361 cgcagtgcca taactactgg actattgttc ctgaaccctg ggtaaaggca gccatggagc
    21421 gcgacaagaa aattcttttc agtaacaggc caaacgacta attcccatga acttttacga
    21481 taccgttaaa cagtttgacg atggccggat gctcccgctt gtggaggagt tttataccct
    21541 acagggtgag ggctatcaga ctggtagggc tgcatacttc attcgcattg gtggttgcga
    21601 tgtgggttgt tcctggtgcg attccaagct gtcgtggaac ccggaggtat tccctcccgt
    21661 tagggttgat gaaatagttg agcgggcagc aagttttcct gcaaaagcag ttgtggttac
    21721 cggaggtgag ccaacccttt acccgctcga ttatctatgc actgaactca agaagcgggg
    21781 tgttcaaacc tttattgaaa cttccggggc ctataccctt accggccaat gggattggat
    21841 ttgcctatcg cctaaaaggc agcagccacc cactgagggc attcacatga tggccgatga
    21901 gctaaaagta atagtatcca ctcctgccga ctttgaatgg gctgaggaga atgccgccaa
    21961 ggtaaaagca acctgccagc tttttctcca gcctgagtgg agcgtttaca atcaaattat
    22021 tccggccatt gttgactaca ttatgctcca tcccaagtgg cgagtttcgc ttcaggcgca
    22081 taagtttatg aggatacctt agcctgaaat aagtaaaaac taaatgctct tctgaatctt
    22141 ttaacatcac cgcggagcat actaattttt acccttttgg gaaaatgtgt atttttgact
    22201 aaaaagtcaa aatgaacgtt tcatttacca tcctttccat tggagcaata atctttttag
    22261 ctcactggtt ttccgggatt ttcgaccgga aagggattcc cgatgtgcta cttctgatgc
    22321 ttgtgggtat tctgtttggc cctatcctga aattggtaaa acccgatgat tttggaattg
    22381 ccgggcccat gttcacatcg ttaacgctgg taatcatact ttttgagggt ggaattggtt
    22441 tgaatataag cgagttaagg aaatcgttgg gaggttcgct aacgcttact gcaataaact
    22501 ttgttgtaac taccattgct gtatcaattc tatcatacat gctgtttcat ctttcagtgt
    22561 tagagtcgct aacacttggt gcaactctgg gtggaacctc atcggcagtg gttattccca
    22621 tggtaaggca gatatcgatg caggagatat ccagaaccta tctggtattg gagtcggcac
    22681 tcagcgatgt gctttgcatt gtgtttgcac tggcttttat tgagggaatt gttggaggcg
    22741 aagttcagcc aggattaata gctggcaaga taatttcatc gtttgttctt gcatcgtttg
    22801 ttggttttgc ttcagccgtg gtttgggctg ttctgctaaa ccggatgcgc tcaatcaaaa
    22861 actccatttt tactacgccg gcttttgttt ttatagttta cggattggct gagatattag
    22921 gatatagcgg ggctatatca tcgttggttt ttggaataac ccttgttaat accgagcagc
    22981 ttaggggcac atttctaaac aagtatttgc cgcaaaatcc tgtttcgctc aacgaaaccg
    23041 aaaaggtctt cttctcggag attgtttttc tgcttaaaac cttctttttc gtatatatag
    23101 gcatttcaat tcgcttcgat aatctgatga ttctggggct aggtttgctg gttgcattat
    23161 cgctttacat aatcagaatt ccggtggtaa agcttacagc ccataatgga attcaggttg
    23221 ctgataaggt taccatgtcg gtaatgatac ctaaagggtt agccgctgcc gtaatggcgg
    23281 gaataccgtt gcaaaaaggg attccgggag gacaggtcat ccaggactta acttactcta
    23341 tcatcttaat tactatcata cttactagcg tgctaattcc tcttttaggt cgatataaaa
    23401 gattgaacgc tatttacggt tggattttta ggattcggtt taataaacgt taagcaactc
    23461 cctggtcatt aaaaaatatt ctcaggttta caatcatttt tttacttttg cttaattttt
    23521 ttgatgtaaa tatggaacag cgcgaatttt acgttgagct gctaaacaag catgtttcaa
    23581 accctaaaat gatagcccat tgcctggctt cggaggctgt gttgcgcgag ttggcccgga
    23641 agtttggtga ggatgaggat gtttggggta tagccggttt gcttcacgac ttggatgttg
    23701 aaataaccaa cgccgatcct aaaagccatg cgctggtagc tgctgaaatg cttcagaata
    23761 tattgccagc cgaggctgtt gatgctatta aaatgcacaa cgaggaggca actggcatgc
    23821 ctcgtactac ccgttttcag catgccctgg ccgccggcga aaccataacc ggcttaattt
    23881 ttgccaccgc actggtttac cccgataagc gaatagcaag cgtgaagcca aagtcagtgg
    23941 taaaacgtat gaaggaaaaa cagtttgcag cctctgtaaa ccgcgataca gtaatggagt
    24001 gcgagaggat tgggcttagt ttggatgagt ttgttcagct ctcgctaaac gctttaatcc
    24061 caattgcacc aactttaggt ttttaatggt tcgatgatga atattgatat agtacttcct
    24121 caggtaattg gtgctgcaca aagggcaggt gagtgggcat tgtccgaaag ggaaaagttt
    24181 acaaccgata tggttgaggt gaagggaaag cataactttg tttcgtatgt cgataagcat
    24241 tgcgaggcca tgctggttga agcattgggc aaagcgcttc cacaagcagg ttttattgcc
    24301 gaggagggaa ccggtagcaa aaattccagc ggtttaaact ggataatcga cccgctcgat
    24361 ggcaccacaa actacatcca tggactatcg ccttttgctg taagcattgc attgatggac
    24421 aatgataggg ttgttcttgg tgtagtgtat gaggcaagcc taagggagtg cttttgggca
    24481 accgaaagtg gtggtgcttt cctgaacgga aaccccatta aggtaaccac tacacaaaag
    24541 gtaaacgatt cgcttattgc aactggtttc ccgtactacg attactcccg catcagcccc
    24601 tttttccaga ccatggatta cttcatgcgc aactcgcacg gcttacgccg tctgggttca
    24661 gctgctaccg acctggttta cgttgcttgt ggccgtttcg atgcgtttta cgagtatggc
    24721 ctcagcccct gggatgttgc tgctggtgct tttattgttg aaagggctgg tggtaaggtg
    24781 tgcgatttta agggcggaaa tagttacatt tttggaggtg aaataattgc aactaattta
    24841 aacattcatc aagagtttac atccgtaatt cgcggtatta tgtttggcta gcctatgaga
    24901 aaagtgattc atgccatatc gttctacact tttagtatac taacattccc tttcaaattc
    24961 atgcccttgc gctggcttta tgggatatcg gatatcatgt accttatcat ttataaaatt
    25021 atagggtata gggttgatgt tgtgaaaaaa aacctgaaaa attctttccc tcaaaaaaca
    25081 gaaagtgagt tgctggaaat tgaaaggaag ttttaccgcc acttctgcga tttgtttgtg
    25141 gagcagtttt acattctgca tgccaatgct aaaaaagcca aacggttatg tagttttaaa
    25201 aatttagagc aacttgaccg gtattacaat gaaaacaaaa gcattgttgt ggctggcgga
    25261 cattatggga attgggaatt gtatggtttg tttgggcttt ttttaaagca tcaagttttt
    25321 ggtgcatata aaccattagc taataagtac tttgagagtt ttgtaaatgc ttcgcgagag
    25381 cgatttggtg cgctggctgt tcccatgaga gacactccca gaaccgcaat gcaatttgcc
    25441 agggaaggga aactattctt tctgggatta atcgccgatc aaaccccagc taagggcgaa
    25501 ataaggtact ggacaacctt tttaaaccag gatacacctg ttttcctggg taccgagaag
    25561 attgccaaga aactcaacca gccggttttc ttctgcaaca tgcgaaagct taaaaggggt
    25621 ttctatgagg ttgaacttga actcttaacc gataaacctg ctgaaaccaa gccctacgaa
    25681 ataaccgaaa tgcacgtaag agcattagag cgattgatta acgaggctcc tgaatactgg
    25741 ctttggtcgc accgacgctg gaaacactcc cgtaacttaa ctcaaactcc caatgagcag
    25801 tagtaaagtt gcggtggtta ttctgaactg gaatggggaa catttcctga aaaagttcct
    25861 tcctacgctt attgagaaca ctccaaggtc tcaggttgaa atagtggttg ccgacaatgg
    25921 ttcaaccgat ggctcgcttt tgatacttaa tgattaccca acggttcgga caattctgct
    25981 cgataaaaac tttggttaca ccggtggata caaccgtgcc cttagccagg tagaggctca
    26041 gtattacgtg ctcctgaact ccgatataga agtacccagt ggatggttgg agcccatggt
    26101 tacatttatg gataaaaacc cccaagtggc cgcgtgcgct cctaagctaa ttgattttaa
    26161 ccacaaggaa tactttgagt acgctggggc agcaggaggt tttatcgatc tgtttggtta
    26221 tccattctgc cggggacgta tcctttccga gattgaagcc gacaatggcc agtacgacga
    26281 tcccatgcca atattctggg caagcggagc ttgcatgatg gttcgctcat catgtttctg
    26341 ggaggctgga ggtttcgatg acgatttttt tgcccacatg gaggaaattg atttgtgctg
    26401 gcgcttgcag aataggggct acataattta ctccattccc agctcggtgg tttaccatgt
    26461 tggaggggga acactgccta acaataaccc cttcaagctt ttcctgaact acaggaataa
    26521 cctttccatg ttgcataaaa acctttcact tagggcgctc cttgtggtta tccctgtaag
    26581 aatggtgctt gatattcttt cggcttttgt ttaccttgtt aatggtcgcc tttcgtttac
    26641 taatgctgtg tttaaggctc acaaacagta tttagccaac atctctactt taaataagaa
    26701 acggagactc cttaaacctt tcaatcaggc tacccgctac aggggattaa ttctagttga
    26761 ttttttcata ctccgtcgtc gtaaatttta tagcttgcca atttctggta acaattatta
    26821 accattcaaa aacccacatt tcaaacgatt gttccaatcg ctttgtcgtt tatcacatgt
    26881 aaaaaacgaa catttagtta gatattgtcg ttaataggta tatatttgca tcttggatat
    26941 cgatgcattg cctttagagt tttacgtaac acatatttat ggtttcattt aagaagttaa
    27001 caattaggga tatactgttc cgttcgcgaa tggcatattc caacaagcca tcattagcat
    27061 ttgttggtca ggagcctgta acctatgccg atatggcagt aatagtccga aatgtttcgg
    27121 gcatgctaaa cacccttggt ataggcaaag gcgataaggt ggcactaatt ggcgagaata
    27181 gtcccaactg gggaatggcc taccttgcca ttacatcaat gggtgcagta gtggtgccaa
    27241 ttcttcctga cttttcgggg cacgagatgg aaagtattat taaccatagc gaagctaagg
    27301 ttgtttttgt ctcagccaag ctatatggtc aactcgatag aaagaacatt ccgggtgtaa
    27361 ttgcttacat actaatcaat aacctacagc cctttgacga gagcgttacc acgcctacgg
    27421 ctggtatgcc ttacgaaacg ccaaaggcta ttgtgaatgt tgattactgc gattgcgaac
    27481 tacctcgccc cgatgaggat gaccttgcag caattattta cacatcgggt actactggtc
    27541 gcccaaaggg tgtaatgctc acccataaaa acctggtgtc aaacgtacta tcaaccctga
    27601 tgatacagga ggtaaacgaa aacgacaggt tgttatccat ccttccgctt tcgcatacct
    27661 atgagtgtgc tataggcttc ttaataccga tggcaaccgg ggcatcggtt tactatctgg
    27721 ataagccgcc tacaccagcc gtacttattc ccgccatgca ggctgttaag cctaccatgt
    27781 tgctttcagt acccctagta attgaaaaga tttacaagaa taagattaag cctgaactta
    27841 catcaagctc tctcaagcga aaactttaca gctttacccc tacccgcaag ctgcttcacc
    27901 ggattgcagc aaaaaaactc cataaaacat ttggtgggga gcttcacttt tttggcattg
    27961 gaggtgcaaa gttgtcgttt gaggctgaac gctttctgaa cgagggccgt ttcccgtatt
    28021 ccattggtta cggtatgacc gaaacatcgc cacttttaac gggaagttca ccaaagctgg
    28081 ttaggttccg tacagcaggt ttttcaattc ccggacagga actgaagttg ctaaacccta
    28141 acccacatac aggggagggt gagataattg taaggggtgc caatgtaatg gttggctact
    28201 acaaggatcc tgaacttacc caaaccatgt ttattgacgg ttggctccgt actggcgatt
    28261 tagggtatat tgacagcgat ggatacgttg aaattcgtgg acgctcaaag aatatgatcc
    28321 ttaccgcttc cggcgaaaat atttatcccg aagagattga agacgtaatc aacaagcatg
    28381 agtttgtgct tgaatcgctg gtttacgagc ttcgtggaaa acttgtggct cgtgtccacc
    28441 tgaacaagga agctcttgag aaatactacg agaatcttaa acattctgcc aaggattggc
    28501 aacacaatgc tgaggatcat acccgaaagg tgttagacga tataatgacc tatgtaaatg
    28561 ctaaggtttc aaagttctcc cgattgaatc atattgttga gcaacaggaa ccctttgtaa
    28621 agacacctac tcaaaaaatt aaacgattcc tttacaagga gtaaaattca ttaatgaagt
    28681 aaaagtcaaa ggctgccatt ggcagccttt ggtgttcaaa atgagttagt gtacgttacc
    28741 taatcttact agtgtttagg caataaggct aaacctgaat ggttaaaaac agattttggc
    28801 cgttccctca ggttgtaatc cgaggccatg gattccgcat aggcgccaca ggaatggata
    28861 acaagcaaat cgccacgttc tgtattgggg aggtttacct gctcgccaaa acaatcggtt
    28921 gtttcgcaaa ttggcccaac cacatcgtag gtggcaatag tagggtattc aattttactt
    28981 atgttctcaa tgcggtgtat ggcgccatag agtgccggac ggattagggc attcatgcca
    29041 gcatctacaa tggcaaagtt tcgcgattta gtagttttgg tatacagcac tcgggtgtat
    29101 agctgtccac attgaccaac cagtgagcgg ccaagctcaa aatggatagt ttgctccttt
    29161 ccggggttga gccactggtt gatggttttg aaataccctg caaagtcggc tatcctatta
    29221 atcctgggat tgatatagtc aattcccaaa cctccaccca ggtttaggta agggagtgct
    29281 ttgctttgta acctttcctg aatacggtta acctcaaggg ctaggttctt gaacacttcc
    29341 atgttcatta tctgtgaacc aatatgaaag tgtagtccaa ccaggttgat attcttatac
    29401 ttatttgcag agtttaatat caaacttgcc tcttccgcac tcattccaaa cttattctct
    29461 tttagtccgg tggtaattga cttgtgggtg ttggcgtcca cattagggtt gatgcgaagc
    29521 gctatactgg cagttttgtt caacttttgt gcaatctgtt caattacctg aaattcctga
    29581 accgactcgc aatggatgca ttgaatattc tgctcaatgg catactcaat ttcccaatcg
    29641 gttttaccaa ctccggcaaa cacaatgcct tggggcttaa atccgttttt cagggcaact
    29701 ttaatctcgt ttccgctaac acaatcggcg ccaaaacctg cacccgaaat gatcttcaaa
    29761 agggcagggt ttgcattagc tttaagtgcg taatgaatag tgtacccata ccttgctgct
    29821 tgcgcagctg ctgcgttcag tgtttcgttc agtagcgcta aatcgtactg gtagaatgga
    29881 gtttgcatgg ctaaaaggtg tttgcttcaa tgttctggtg ggttgaatca cttacactca
    29941 ccgccgggaa aagctgctgt agcaacctta aggcctgaac cttatagtgc gaatcaatca
    30001 gaaacgagat attgtttacg cttcctccgt aggagatcat ccgtataggt atctcgcgca
    30061 ggcaatcgaa aaccatggct gcatagccct tatgcgatgc tgggaaatcg ccaactacgc
    30121 aaacaatggt ttggttccgt tccacttcca cctgtgcaaa ttggcttaaa gcatttacaa
    30181 tcttttcaag gttaatagtg ctatcaatgg taagcgatat ggatacctcc gatgtagtaa
    30241 tcatatcgac aggggttttg tatgtgtcaa aaacctcaaa aaccctgtgc aggaagccat
    30301 aggctaaaag catccgcgac gacttgatgt taattgctgt aataccatcc tttgctgcca
    30361 ctgcctttat tcctttccgg ttctttatgg atgatattac cgtcccttcg tctgtaaggt
    30421 ttaaagtgtt cttcaacaca accgggatat tctttacctt gcagggttga atggtttggg
    30481 ggtgaagtat ttttgcgcca aagtaggcaa gttcggcagc ctcgtcgtac gatagctgtc
    30541 gcaatgggaa agtatttgta acaaagcgtg ggtcgttgtt gtgtacccca tctatatcgg
    30601 tccaaatttg cacctcgttt gcgttcagca catttccaac aatggctgcg gtaagatcgc
    30661 ttccacctcg gcccaggtta tcaaccaacc catttgcatt gcggcaaata aaaccctcgg
    30721 ttacataaat ttttgcaaaa gggttatttt gcatgaggcg ttttaggttc tgttcaatgt
    30781 aaaagaaatc gggctcgtta tccttgtcaa tcctcatgta ttccagtgct ggaatgtacg
    30841 cggctctttt tccctgctct gttagtagca tgtggaatag ccctaccgat agttgctctc
    30901 ctagggctac tatattccgc gaatcggtct ggttgaagcc ttcaatcaat ctcgatagaa
    30961 ttcgcagaaa caggttgcta ataaattctt cacccctttt tcggtacatt tttgtatcaa
    31021 atagtctgtc ggcggttttt atgtgcttgt cctcaagctc tttagttttt tcaattgcct
    31081 cgagcatctt acccgactgt gccagctctg tgatttccac taagctgttt gtaactcctg
    31141 ccatggccga taaaaccact acaatgggtt catcgccatt aagcagctcg cttacctttc
    31201 tgagcctatc ggcattacca accgatgttc cgccaaactt taaaactttc attttctttt
    31261 tttctgattt tttaggggtt taacataaaa aaaggcctgc aggtttctgc aggcctaaca
    31321 ctcaatatca ttgtaatact tttatgctga ttttatcaat gtgttaacgg cctgcttgtg
    31381 ggtctgtttt ttcatggtaa taaccatctg tttcatgcaa atggtttgca tgaaaaactg
    31441 tttattactc atggtatagc aagccgaaaa ttccattttt gtgctatttc acttttgcaa
    31501 aaatactttt ttttaatatc aaaccagctt ttaggaaaaa atgtttttat tttttatgaa
    31561 gcacttttgt gggtacgatt gataaatgtt acttttgttg gctaaaattg attgacaaca
    31621 agttgactaa atcttttctg tatgaaaatt tcgcatagct ggcttacgca atacctgaat
    31681 gtgagtaaaa cacccgatga gatttcaaag atactaacat caattggatt agaggttgaa
    31741 agccttgagg caaaggaaac cattaaaggt ggccttgccg gggttgtggt gggcgaggtt
    31801 ttaacatgcg aaaagcatcc cgatgccgat aagctgagct taaccacggt aaacgttggt
    31861 gtcgaccaac ccttaagcat tgtatgcggt gcacccaatg tggccaaagg ccaaaaggta
    31921 ctggttgcaa ccgttggagc caagctgtat ttcaacgatc aggaaatagt aattaaaaag
    31981 gcaaaacttc gcggtcagct ctccgagggt atgatatgtg ccgaggatga gttgggttta
    32041 ggcgatagcc acgatggtat aatggtttta cctgccgatg ctcctgtggg catgcctgct
    32101 gctgattacc taagaattga gaaagattac cagtacgaga taggcttaac ccctaaccga
    32161 attgatgctg cctcgcatta tggtgtggct agggatttgg ctgcctacct aacctaccat
    32221 gagagcccgg ttaaggccaa tcttcctgat gtttccaatt ttaaggttga taacaacaca
    32281 ttgcccatac ccgttgaggt tttgaacact gaggcatgcc cgcgttactg cggtatcacc
    32341 attaacgggg taaaggttgc cccttcgcct gagtggttgc agaaacgatt acgttcaatt
    32401 gggctcaatc ccattaataa tgtggttgat gttaccaatt tcgttctgca tgaggttggt
    32461 caaccactcc atgcttttga tgccgatgcc attaagggca acaaggtgat tgttagaacc
    32521 atgcctgagg gtaccccttt tgttaccctc gacggagtgg aacgcaagct atcagccgat
    32581 gatttgatga tttgcaacga gagtgagccc atgtgtattg ccggtgtttt tggtgggttg
    32641 cactcgggag ttactgaaaa gactacaaaa gtattccttg aaagcgcttg ctttaaccct
    32701 gtttacgtcc gtaaaaccgg ccgtaggcac gatttgcata ccgatgcctc gttcaggttt
    32761 gagcgcggta ctgaccccaa tattacagtg tgggcactaa agcgtgcagc gcttttgatt
    32821 aaagaggttg ctggcggtac tatatcgtca gagattgtgg atgtaaatag cggtccggtt
    32881 aaccacttcg aggtggattt caacatccac agggcatgga gtttaattgg aaagcaaatt
    32941 cccgaagttg atatcgaaag aattcttgag ggtcttgaaa ttagggtgct taagaaaaat
    33001 ggacagcagt ggcttttagg agttcctaca tacagggtag atgttcgtcg tgaagccgat
    33061 gtggttgagg atattctcag gatttacggc tataataacg ttgaaatagg cgctaaggtt
    33121 aatggaacat taagttacac caaaaagccc gatagccttc agatgcagaa cattgtatcg
    33181 gactaccttt cgtcaaatgg ttttgctgaa attatgtgca actcgttgac aaagggcggt
    33241 tactatgaag ggctggctac tttccccgag gtaaactcag ttaagattct taaccctcta
    33301 agcaacgagc tgaatgtttt acgccaaaca ctcctttttg gagggctcga gagcattcag
    33361 cgaaacatta atttccgtaa cctaaacctt aagctctacg agtttgggaa ctgctatgta
    33421 ttcaagcccg aagtaaaatc gcagaaaccg cttgataagt actcggagca ttttagcatt
    33481 ggcatttggt taaccggcga taatattaag gaacactgga tgaccaagcc cgaaaggcag
    33541 aatttttatc acctgaaggg ttatgttcat tccatactat cgcgctttgg ttttagtatt
    33601 gatagaattg aagcatcaga ggctcctggt gatatttacg cttatggatt aacctacaag
    33661 gcacagggta agcatcttgt tacatatgga atggtaagcc cagaaatact caggcaattc
    33721 gatataaaga ccgaagtttt ttttgccgaa ataatgtggc tgcctttaat ggatatgatt
    33781 gcagaaagca aaattcagta ccaggaatta cccaagttcc ctgaggttcg tcgcgattta
    33841 gctttgctta tcgattcatc ggtaaagtat gagcaaatcc gaaagctagc ctttgaaacc
    33901 gagcgcaagc tcctaaaaag cgtaaacctt ttcgatgttt atcaagggaa gaatatacca
    33961 gaggggaaga agtcgtatgc aatcagttat atccttcagg acgaaagcaa aacccttacc
    34021 gacgagcaaa tagataggac aatgaagcgc ctaattgatg tttacgttag ggagcttggt
    34081 gcgcaaatcc gttaaaagta aactatggcc aaaattgaat tggtgttagg tgatataacc
    34141 caaatggatg ttgatgccat tgtaaatgca gcaaacgagt cgttgttggg tgggggtggt
    34201 gtagatgggg ctatacatcg tgctgctggc ccgcagctgc tcgaggagtg tagaaccata
    34261 ggggggtgcc ccacgggtga agcgcgaatc accaaaggat ataacctaaa ggcacggtat
    34321 gtaatccata ccgttgggcc aatttggagg ggtggccagc acaacgaaac gttactactc
    34381 cggcatgctt acgttaacag cctaaagtta gcgccaaagc atgggttaaa aagcattgct
    34441 ttcccaaata ttagtaccgg ggtttaccat ttcccaaagc aatcggctgc acaaattgcc
    34501 ataagggcag ttatggaatt cactgctaaa aaccatttgc ctgagcggat tgtatttgtt
    34561 tgttttgatc aggagaacta ttctatttac agtgaacttc taggcaagta agtttatagg
    34621 tttatatcgt atttttccag ccttcggtct agtgcctgac gggtaatatt taacaactgt
    34681 gctgcctcgg ttttcttgta acccgtacgc tcaagggctt tcattatcac tatcttttca
    34741 attagatcga gatcaaagat atcctctcca attattcctg ttgtgctgac tgaatcggtt
    34801 ggagctatta gaccgctaaa gtttttcatg gttaatctgc cgccctcgga gagaattatg
    34861 gcacgttcaa ccatattacg catctccctg atgttgcccg ggaagttgta acccatcatg
    34921 gccttaacta tggttgggtc aacctgggtt atctttttgc ccattcgggt tgaaatgtgt
    34981 ttaacgtagt aatcgaaaag gattggtatg tctacctttc gctcgcggag cggaggtatg
    35041 tgtatttgga atgagttcag gcggtagtaa agatcgctgc ggaacttacc ctcttcaata
    35101 agtttgttaa tgtcctggtt ggtggctgca ataattcgca catcaacggg gatatccaca
    35161 cttgagccaa ttctacgaat ttttcgttct tccaggacgc gtagtaactt actctgcatg
    35221 gcaggttgca tttcaaccac ctcgtcgagg aagagcgtac caccatttgc cacctcaaac
    35281 cagccagctt tatcctcgtt ggctccggta aatgcccctt tcttgtgacc aaaaaattcg
    35341 ctctcgaaca gggtgtcggg aatggctgag cagttcactg cgtagaaata cgatttttta
    35401 cgtgagctaa ggtagtgaat gcctcgggca acaagttcct ttccggttcc gctctcgccg
    35461 gttataagta ctgaggtgtt gtcggtttta gccaccttgc ccatgaggtc aactacctgt
    35521 ctaattccaa aggagttacc aataatctcg gtaccaatgc tttcaatcaa atcctttgat
    35581 accaaagagt atgtttggtt aatctctttt agctgctcgt gtagggcgat atagcgtttg
    35641 gtgcgttgaa tggcagctcg catgtcgagc aagcggaagg gttttgggaa gaactcaata
    35701 gctcctaagc gcatagcatt aataactgca tccatgtcgc cgtgcccagt aatcatgata
    35761 acctcaatgt cggggtattc ctcctttacc ttggtgagca cctgtaggcc atccatctcg
    35821 gggaggcgga ggtcaaggat aagtatatct atttggttct cgctgaggat gctgaatgct
    35881 tccgacggtc gctctgctgt aagaacggaa aagccttccc catccagaaa ctcttttatc
    35941 tctttacgaa aaaccttttc gtcgtcgaga accaaaatgc ttatctcttt catcattcta
    36001 taatgatttg tggtttatta ctctctagga attgatattt caaaaagggt gtattcatta
    36061 ggttcgcttt caacgcgtat atcgcctaaa aggtctttga taaacccata cgatatggat
    36121 aaccctaaac cagtcccttt gtcctcgctt ttggtggtga aaaatgggtc gaaaattttc
    36181 tctacaattt tttggggaat gcccgttccg ttgtcccaaa ctgttaggta taggttttgc
    36241 tcatctttac gtgtgccaat tttaatcttt tttcgatagg tcttatcctc acttttcttt
    36301 tctttttcat caactgcata ctttgcattg gaaaggaggt taagcaaaac ctgctctaac
    36361 ttatacttgt cagccgtaat tggtggaatg ttttcatcca atgaggtttc aatgataacc
    36421 ccatggtttt caaattgagc cgataccata aagagggcac tttttaccac atcgtttagg
    36481 ttgaccgttg aaaaagaaac aggcttctga gtacgggaaa aatagcggat gtggtcaatt
    36541 atctttttaa tacgctcaac attctcaaac agcgagttta gcttctcctt taggtactcc
    36601 tcgctacagt ttttgtcctg aatacgcata aggatattgt ccagccccat cgatatacca
    36661 cccagtggct gattgatttc atgggcaata ccggctgcaa gggtaccaag cgattcaagt
    36721 tttgactttt gcgagagcaa ttgctgttgc ttttcctgtt tctttatctc ttcaatcatt
    36781 cgccgttcaa gctctcggtt tatctcggtg atacgctgtt ccgtttcctt ttgcttagca
    36841 atctctttct gtttattgcg gaatagcaca accagtagcg agagtattat tgccacaacc
    36901 acaagcatcg atatcagaaa acgcatttgg tacctcgact tcgctatctg taacttttgg
    36961 atctcggagt tttgctttag gatttcgttc tctttttcct tttgcgatat ctcgtaaagt
    37021 atttggagtt tcgccagtcg ttgtcctgtt tcttcgtact gcaatgaatc gcgcaggtca
    37081 acccacatct tgtggtaatc aagggcatcc ttgtagcgcc cctgagcctc attaattact
    37141 gaaaggtatc ggtaaacatc agcttttgaa gaggtaaggt tgtttttctg tgctatgtta
    37201 agtgcttggt ttaggaagcg cgatgatgtg gggtaatcgc caagttgaaa gtggcaacgc
    37261 gatatgctta ccaaagcagc tattaaggtt ggtatgtcgt tgtattttga ggaaatgctt
    37321 tggcactggg tgtagtagtt tatggcctgg ttatagttcc ctttctcata gtagtagtcg
    37381 cccagtccct gtaaactttg gataattcca acggtatcgg aagcattcag gctaatggtg
    37441 tacgataggt tgaagtactg ataggcgcta tcgtactttt gcgtaagcat aaaaaccact
    37501 ccaatttcat tgagcgattt gctgtacgaa gttgaatcgg attgtttaat agcagcctcg
    37561 ctaacctttt taaagtaatc gagactagtt cctaaatcgc ctgagttttt actggcttgg
    37621 ccaagtaggg tgtaggtatc aatcagggct ttgtttttat tgtaaacaga ggtagcttgg
    37681 ttgtttagca gggttagggc ttcaattcca tattgctttg cctgcttaaa gttcttttgg
    37741 cttaaatgga ggttgccgag cttaagtatt agctcaaggt aaacattttt atttggaata
    37801 ttttttagtg tttttatgcc cgcttcgagc tgttgtattg ccaggtcgag attttggctt
    37861 gcttcgtaaa ccctagccat tcccaaaagc gattttgctg caaactcggg tttattgatt
    37921 gatgagtaaa ggttggcagc ccggttaaag taacggagcg aggtttcgta ctcgccaagc
    37981 ttatagtttg cttcggcaag cagctctagg gccatcgatt ccgtaaagga atcaccttcc
    38041 tttttagccg ttacaagggc ttgtaaccca atatcaaggc agcgatcagg gttggttttt
    38101 agcagggatt taccctgaac cacaaggtca gcggcttttg agctaccctt tacagcaatc
    38161 tcttggggca aagtatcggt ttggcttagg ccaatttgcg ctaaaccagc aaggcttaat
    38221 gtaagccata ctattcgtag taaatgtttc atctaacttt gctgatgcta atatagctat
    38281 attccaaaaa ttgcctactt ttatagcctt gaataattta tgctagtatg ttttttaaag
    38341 taaagaacta cctgtcgctg gtaaagttca gccatacaat ttttgcaatg ccttttgccc
    38401 ttattggtta ttttttagct tactataagc actcgctaaa ccctgattgg tggctttttt
    38461 taaaggtgat actctgcatg gtttttgccc gaaatgcagc tatgagtttc aaccgtttag
    38521 ttgataggca ttttgataag ttaaatccac gtacaagtgg gcgggaaatt cctaggggta
    38581 tcattgctcc caggtctgtt atgcttttct gcattgtcaa ttccatcctt ttcattttaa
    38641 ctacattctt cattaaccca ttggtgtttt acctgtcgcc tatagcgttg ggtgtcatcc
    38701 ttttttatag ttacaccaag cggtttacca tgttcagcca tcttgttctg ggaataggcc
    38761 tatcgcttgc gccaattgga gcataccttt cggttactgg tagttttgct cttattccta
    38821 ttctatactc ggttgttgtt cttttctggg tggcaggctt cgatatcatt tatgctcttc
    38881 aggatgagga gtttgaccgt aattccaagt tgcactcaat tcctgctgca gttggtgctc
    38941 gcggggcttt ggtcattgct ttggtgttgc attttatgag tgcattaata gttatagcca
    39001 ttgggatact ggctggcttt aacatttact actggattgg agcattcctt tttatagctt
    39061 taatgattta tcagcattac cttgtccgtc caaccgatat ttctaaggtg aacatggcct
    39121 ttgccaccac caatggaatg gcaagccttc tgttcgcatt gtttaatata atttcgatat
    39181 taacttgcta agagttatga aacacccatg gaaggcagct tgcacaaaca cagatgaaca
    39241 accgagcgtt ttttgcgagc tcgacgaagt caaactatgg gtgttccatc cgctcgataa
    39301 agtttgttaa aagcaagaga gtaacgagtt agtaattaat gatataaata ttttgtactg
    39361 atgaaaaaat atattgtatt ctttggtctt ctatcactaa tattgggctg ttccggtaac
    39421 caaaatagtg ctaacaaagt ggagctggtt gatggtcagc cagttattga tgtaacggtc
    39481 gattcgttac ttttaaacgc taagaattac gagagtaagg tggttcgtgt tagcggtatt
    39541 gttgagcatg tgtgtgttca taccggcttg cggattacgc ttcaaggcaa atcgccggaa
    39601 atgaagctga gtgtaaaaac atcggatgcc agtcggcctt ttgatatgtc aatgaaagga
    39661 gcaaaggcta cggtcactgg tgttttcaag agggtagggg gagcaaacga ggtctgcgat
    39721 gtggaatcgg ctcaccatcc aggtgaaatt tactttttgg agtgtaacag cattaatgtt
    39781 aagtaaatat gacactttca aagttcttgc gggtaattca cagggattgc agctactttt
    39841 ttgccggaat ggttttgcta tactctgtaa caggaattct cttaaaccac gggcatcagc
    39901 ttaaccccta ctactacatc gatgttaaaa cagtaaaggt tgatatccct agggattcgt
    39961 caaaggttaa cgaccagttt cttttaggtg tcttcagtcc atttggcgaa agcgagatta
    40021 ggtcgcatta ctaccccgat tcatcaaccc tgaaagtttt tttcaagaac ggaaccgcca
    40081 cggtaaacct gaatgagcaa accgcgctaa tcgaaaatag tatacggcga ccaattctat
    40141 atcagctcaa catgatgcat cgtaaccgac ccaaggcatg gtggacatgg tttagcgata
    40201 tttttgccgt tgggctaata gctattacat taacggggct tttcctgata aaggggaaaa
    40261 atggaattac ccggcgaggt gcagtgctgg ttgccgcagg ccttattttg cccatactgg
    40321 ccatgatcat tttcttttaa acgagagcaa atacaataca tttacataag gggggctttt
    40381 gtcgttctaa aatcaatgtc gtttagttaa caaaaatata catgcctgtc ttcctgagcg
    40441 cagcgaagga tctctaatcg accccatgag acgtttcgct cgcgctcaac atgacaatag
    40501 cttcacgaac aacgctccca gccccgtagg ggcgaaacat ttgtaactcc atggctactc
    40561 cctcatgtgt tgcgatgcac gcaatacatt ttccaaagag cagagggaaa gcgttgcatc
    40621 gcaatagaag agcacggcaa agagcggttt tccaaaaaaa atacaagaat taaagggttt
    40681 aaggtaaaga gatgcttcgg ctagctcagc atgacaaggg tgcagaaagg aagatagggt
    40741 ttatgtcatc cccaacaaag tgagggatct cttcaaagga ttgaagaaaa tcagcaatga
    40801 gatacttcgc gaagcctatc ccgataaatc gggactcagc acgacagcat tatgtttcgc
    40861 ttgcgagcaa tatgacaaat aactttaact aaataacatt gattttaaaa tcgttttcga
    40921 tttgtaattt ttttattttt gcatgagttg aaaatctacg agagtgatat ttaaggaaaa
    40981 catataccta aagtttactg ctaagtacgt gtttctgaat ataaagaacc gcgataggtt
    41041 ctaatttttt ccacccagcc ataccggcat ttacattttt cgcctttggt gaaaacatct
    41101 gcttttataa atcgatatca gtttaacaat taaaatattt attagtatgg aactaccatt
    41161 tgccgaatcg tacaagatta aaatggttga acccattcga cgtagtaccc gtcaggagcg
    41221 ggaacaatgg ataaaagagg caaagtacaa ccttttcaat ttgcccagca atcaggttta
    41281 catcgatttg ctgaccgatt caggaaccgg ggctatgagc gaccgccagt ggagcgccat
    41341 aatgcttggc gatgagagct atgctggggc ttcctcatac ttcaacctga agaacgccat
    41401 caggaacatc cttggttttg actacataat gccaactcac cagggacgtg ctgccgagaa
    41461 tgttcttttc tcggtactag tgaaagaggg cgattacatc cctggcaact cccacttcga
    41521 taccactaag ggtcatattg agttccgccg tgcccacgct gtggattgca ccatcgacga
    41581 ggctgccgat actgaactca ttcacccttt caagggcaat gtcgatcccc aaaagctcga
    41641 taattttctt aaggagcatt cccaaagggt tccatttgtt atagtaacta ttaccaacaa
    41701 taccgccggc ggacagcccg tatcaatgaa aaatcttcgt gaggttaggg agatatgtaa
    41761 ccgttacggc aagcttttag tttttgactc cgctcgtttt gccgagaatg catactttat
    41821 caaaacccga gaggagggct acgcccacaa atccatcaag gagattgtaa gggaaatgtt
    41881 ctcctacgcc gacgccatga ccatgagcag taaaaaggat gccattgtca atattggtgg
    41941 tttcattgct atgcgcgatc aggagctttg gcgtcagtgt tcaaagttca acattatgtt
    42001 tgagggttat gttacctacg ggggaatgtc gggtcgcgat atgaatgctc tggctgttgg
    42061 ccttgacgag ggaaccgagt tcgattacct ggaaacccga atcaagcagg ttgagtacct
    42121 tggtaaccgc ctcgatgagt ttggtattcc ctaccaaaaa cctgccggtg gtcatgccat
    42181 ctttgttgat gccaacaagg tattacccaa tatcccgcgc gaacagttca gagcgcaaac
    42241 acttgccata gagctttacc ttgaggcagg tgttcgtggt gttgagattg gcaccctact
    42301 tgccgaccgt gaccccgaaa cccgtgagaa tcgctatcct aaacttgaac tccttcggct
    42361 tgccattcca cgccgtacct ataccaacaa ccatatggat gtggtggccg ttgcccttaa
    42421 aaacgtttac gataggcgaa accaaataac caaaggactt gaaattgttt gggaagctcc
    42481 tataatgagg cactttaccg tggagcttaa acctgccgag taataccttt attcatattt
    42541 gcaagagccg gatagtaaag tccggctctt ttgcaatttt atcaagtttt tttcattaac
    42601 cttgatttat tgaattacta atcgtgctta aaaacaatac ctgttgcact aaacaccaaa
    42661 aactaaacac aaaacacaaa acacaaaaca ctacctttgt agttctcatt ttttttagcc
    42721 tactttgcaa tgcatttcat taagccatga aagactttac cactggtaat gaggccaaac
    42781 acatttttcg atttgcactg ccaatgctaa ttggcaatat ttttcagcag ctatacaatg
    42841 ttgtagatag tattattgtg ggacgctacc ttggcgataa ggcattggct gcagttgggg
    42901 cttcgttccc aattattttc atggttattg cgcttattat tggtattggc attggctcat
    42961 cggttgtaat ttcgcagtac tttggtgcta aggattttga tagggttaaa cgggcagccg
    43021 ataccaccta tattttcctc ttttttgcag gtattattat taccattgta ggtttgctta
    43081 gtggtgagta tatctttagg ttaatgcgct tacccgagga gattttgcct atggcaaaaa
    43141 cctacctcga tatctacatg tacgggttgg tgctaatgtt tgggtttaac tcagtatcga
    43201 gcatactacg aggcgttggc gattccaaaa caccacttta cttccttatt gtttcaacat
    43261 ttctaaatat ttttctcgat accctttttg ttcttgggtt taagtggggt attgctggcg
    43321 ctgcatgggc tactgtaatt gcacaagcct tttcattcat tatcgcagta atatacttgg
    43381 aaaggctaag gcacatgctt cgcattacct ttaagcgatt ggttttcgac tgggaaatct
    43441 tctggcaaag tgttcgaata ggcttaccca ccggcattca acaaaccctt gtagcattgg
    43501 gaggtatggc attaatgggt atagtaaaca catttgggac tgaggttatt gccggttttt
    43561 cagcagccag ccgaatcgac tcgctagcgg taatccctat aatgaacttc tcagcatcgc
    43621 tttcgggttt tgtggggcag aacattggtg ctggtaaggt tgatagggta aagcgtggcc
    43681 tatgggctac catcaaaatg tcagtgctgt tcagcgtaat tatcactgca atcatactta
    43741 tttggggcga taagatgatg gtagtattca cgacaaaccc aagggttgtt gatattggtt
    43801 atcactacct tatggtggta agcttttttt atgtggcatt tgctgtgatg ttctgcataa
    43861 atggattgct acgcggggca ggggctgcgg ttgtgcccat gtatataaca ctactatcgc
    43921 tctggattgt acgcatacct ctggcatatc tgctaagcaa aacttttggg atgaacgagg
    43981 ctggggtttg gtggtcaatt ccaattggct ggttaatggg tgcagtagct tcaactattt
    44041 attacaggta tggaagctgg aaaaataaat cggttgttaa gcccgtaacc actgaagatg
    44101 atggggtggc taccgatttg gtgtagcaga ttacacagtt ggtgataaca caaaaagtat
    44161 tacctttgca aactgataaa taaaactact gattatggca agcaagagag atcttaaaaa
    44221 ggatatcgat tacctggttg gtgaggttat tagcgattgc tacacctaca tgctaataaa
    44281 tggcgaaaag aaacgcgatg atgcaattgc tatcatcgag tcgattcttg aaaagcgcaa
    44341 cgatcttatt catcgtatta aacatcccgc caaaaaggat gatagaaaag ccgttaaagc
    44401 gcattaccgc gaaattcaga aagatgccct aaaggctatt gatgaaagtt ttagcaaact
    44461 tagcgagctt acaaagtagt attccaaaac accacatatt tcccggccct ttgccgggat
    44521 tttttgtttt atgttttcac agcactgcgg cataaaagaa ttcttaattt tgcagttcaa
    44581 ccatctaatt acataagcca tgattcagga gttcaggcaa tacttttccg atatcaccct
    44641 aaaaggtaac atatacctaa tgcttttgtg gcgatttgct cttttaatgc tgatattcac
    44701 cctttgccgg gtactttttt acctgttcaa tatcgatttt tttgccagcg ttactactgc
    44761 aggccttttg cgaatgcttg ccggtgggct ttggttcgat accagcgctc ttatttacac
    44821 aaacatttta tacatcgttt tattcctttt gccattcact tttaggtata atgaatggta
    44881 ccaaagggta cttaaatatc tgtttgtggt tacaaacagt gtggccattg gcgcaaattg
    44941 tgccgacttt atctactttc gttttaccat gcgtcgtact accgctactg tttttagtga
    45001 gtttaagcac gaaacaaatt ttggttccct tatcccaaag tttattgccg attactggta
    45061 cgttttcctg atatggattg cccttactgc tttactggta ttactgtatg gtagggttcg
    45121 tcctatcaat aagggaaaag gtttcaggtt tcatttagtt tatggcttaa atggccttgt
    45181 gcttttactg gtttttgcta cactcatggt tggtggtatg cgtggtgggt ttcgtcatag
    45241 tactcgtcca ataaccctta gtaatgctgg ccagtatatt aatgagccac ttgaggccgc
    45301 cattgtcctg aatactccct ttgccattta tcgtaccctg gggaaaaagt cgcttgttaa
    45361 ggttgattac tataaaaacc ccaaggaact cgatgaggtt tactctccct tacacaaacc
    45421 acaggtaacc gattcgctga agcgaatgaa tgttgtcatt tttatacttg agtgttttgg
    45481 tagggaatac attggggctt acaataaaca cttaaaggaa aaggatggct ataccggatt
    45541 tactcctttc ctcgattcgc tcattgggca ctcgttatgg tttacgcact cttatggtaa
    45601 tggtcgaaaa tcgattgatg ggatgccctc ggttttggcc agtataccta tgtttgttga
    45661 gccctacttt ttaaccagct actctaccaa caaaatcaat agcattgcat cagccttaag
    45721 ggccgagggt taccatacag ctttctttca cggtgcaccc aatggttcaa tgggttttca
    45781 ggcatttgcc aatgttgctg gatttgagga atactacgga atgagtgagt atcctgaccc
    45841 ctcgcacttc gatggcatgt ggggcgtatg ggatgaggag tttttccagt tctttgcgca
    45901 aacccttaat aagtttccac agcctttctg cgcagcatta ttctcggttt cgtcgcatca
    45961 tccctttaag gtgcctgagc gttacgaggg tgtgttcccc aagggaaccc tgcccattca
    46021 ccagtgcata gcctacaccg attactcgct caaacgtttc tttgaaaccg tttcacgcat
    46081 ggattggtac cccaatacgc tttttgtcat aactgccgac caccctaacc aaacccagtt
    46141 cccggaatat caaacaagcg taggtgcatt tgctgttccc cttatattct acagacccga
    46201 taatagtctt gctggtcaaa aggatgagtt tgcccagcaa atcgatatca tgccgtcagt
    46261 tcttggatat ttacactata gcaagccttt tatagctttt ggacgtgatg ttttcgattc
    46321 cacttccaaa ccattcgtgg ttaactatat taataactgt taccagtatt actccgatga
    46381 ttacgtgctg atttttgacg ggaaaaaaac tttaggcctt tataacatca aggatgatac
    46441 tatgcttaca aacaatctac agggtcaact tcctgatagg gtgaacgaaa tggaaagtaa
    46501 gcttaaagcc atagttcagc agtacaactc aaggatgatt gatgataatc tagtggtaaa
    46561 gtaggtgtta gttgtaaacc ctaacgtaat cgccactttt taaaacctgg tatcttttta
    46621 atgggtaccc ttcctttggg taaccatagt ttacaaggta gtaaatggtt ccgcagtgcg
    46681 gacaggttgc ggtacctgac ccgtttgaat cgggattcac cgatgcgttg atatcgatat
    46741 gttttgggca agttgcatca aaagctaaaa tttcgttctc gctaagcctg aaaacaatta
    46801 caccgttgtt cctgtaaccg tatccatcta ttttaacagc acccgttaca atgttcaggt
    46861 tttgaaactg tggctcattc aggttgaact caatgtttac aggaacatca ggaataatat
    46921 catcaatatt gtcgtcgcag gcagtattag taaaaaccaa aattgaaaaa agcaaaatat
    46981 ggattatctt tgtttgcatt ggtaaattaa atttgtatct tggacaaagg tataaaaaag
    47041 atactttttg cgcttattgc actttttgtt ctggttggtt gtggtaaaca gttagcaccc
    47101 tcgcctgttc ctgccacccc tggtaggagt aagtggcagg ttaaacggga ggaataccga
    47161 cgcgcatggg ttgcatattg gcacgatagg aaaaccgaaa ggttaactcg cggggctaaa
    47221 gcaaaatcgg ttaaaaggga gagggaggct gaaaaggcgg agaaaaagtt ggcggagagg
    47281 cacctcaaaa aacaaccacc tgaggtgcag gaaaggatga aggcttcgct gaaagaggct
    47341 gagaaaaaca gacctaagcg gactttaagg cagaggttag gcttttggaa gtataaatag
    47401 taaaataaat gatatggatg caggcgattt tatatacatt ctcatagcaa tagcattagc
    47461 tatactcaat gcttttgcga atagcaagaa gaaaaaggct gccgagcaaa aacggcaggc
    47521 acaaacttct gcacctgccg atgatgactt gattaccaaa aaattgcagg agttactggg
    47581 tcaggaggtt attgttgaaa atgaagagca acccctggaa tcaactgttg ttaatgagga
    47641 ggaaaagtat atatatgaat acagcagcaa acctgaggtt gaaacattgg ataggagcgt
    47701 tgagttcgaa aaggagccta ttgataagcc actaaccgag gaggaactct accctataca
    47761 gccccagaag cccatcgata cttcaacccc tattgagtat acgcctattg atattgccga
    47821 atcgaaggtg gaaggcccta taggcgatta ctcctaccag gattccttta atgaaagtat
    47881 gcacgaggtt gatattgatg cttccgatgc tgaagaactt gagagtgtta agaaggtgga
    47941 agaagaaaag gaaccccttt tagccgattt tgatccgcta aaggctgttg tttacgccga
    48001 gattattcgg ccaaaatatt tttagttaca acataatggt tggaattttg aattaatttt
    48061 ttatatcttt gcaatcaaag agttccgatt ttcctgtcgg gattcttttt tatttcatat
    48121 ttaggaggta aaatgagtaa agtaacctat ttaaccgagg aagggctcca gaagttgcgc
    48181 aaggagctag agcagcttaa aactgtggaa aggcctgcaa tttcaagaat gattgccgag
    48241 gctcgcgata agggcgattt atcggaaaac gccgagtacg atgctgccaa ggaagcacaa
    48301 ggtatgcttg agctgcgaat ttcaaagctt gaagatttga ttgccaatgc acgcattatt
    48361 gatgagtcaa agattgatac aacccaggtt cagatactga ataaggtaaa gctcaaaaac
    48421 cttaaaacca acgccgtagt ggagtatacc cttgtggctg agtcggaagc taaccttaag
    48481 gagaataagc tttcaatctc tacccctatt ggcaaagccc taattggcaa aaaggttggc
    48541 gatgtggttg atgttcaggt gccttcaggt ttgatgaagt tcgagattct tggaatatca
    48601 ctctaaatca tattgctatg gctacaatat tcagccggat agttagtggt gaaataccct
    48661 cgtacaaggt tgccgaggat gaaaggtttt acgccttcct cgatattaac cccttggcca
    48721 agggccatac cttggtaata cccaaggttg aggttgatta cctcttcgat cttgatcctg
    48781 aaaccctggc tggacttaca ctctttgccc agcgggtggc caaagctatt gaaagggttg
    48841 tgccctgcaa gcggattggc gttgcagtac tcggtcttga ggtgccccat gcgcatatac
    48901 atttagtccc gctgaataag gaatcggata tcgatttcca taaacccaag cttaagctta
    48961 cccctgagga atttattgaa cttgcaaaaa gtattgctgc agagttcaag tagatataat
    49021 aacaataaaa tgataaaacg ggccaagagc ccgtttttta ttacgattat tgcctagagt
    49081 ccctgaaaga cttcctgata tgcattaaag aatgcccatg catcattcct ggtttcggag
    49141 atattctttc cggtttgctt tatgtatttt ttccctacaa ggtcgtaaac tatggtatcg
    49201 gtttggttta cgtagccaaa gccttcgtta aaggcaaagt aggcaaaggg tgtaacctta
    49261 cttgcaagaa tattctttga tagagtaaat tgatcatgag gcaatttaag ctgtgccaga
    49321 agtgttgtgg ccaaatcggt ttgtgagcat gtggttgtga caaccaaggg ttttctttct
    49381 acaaccccgc ccagccaaac cataggaata ccaaacttta agggctccca gttgggatgg
    49441 tttgcaggat gacgatgacc atggtcggca ataagtacaa taagggtgtt tttgaaccac
    49501 tccctttgct gggctttggc tataaaatcg ccaagacagc tatcggcata gtatgccgag
    49561 ctaaggaatt gttccacctc ggtctttcca ttaaacttcg gtttggcggg aatctcaaag
    49621 ggctcatggc tgctaagggt gaagaacatt ttgaagaaag gtgctttggc tgagtcgata
    49681 tcggtaagta ggtaattaaa aacatgctca tcgtgcactc cccacttgga gttgcggtag
    49741 cttttaggaa aatcgtcctg tgttatcagt ctttgaaatt tagcatggta aaggaatgag
    49801 cgaatatttg caaaatcggg gtcgcctccg tagtaaaacg ctgtactgta gccttggttg
    49861 taaagcactt cgctaatggt tggcagtttg ttcgattttg tggggtactt gattaccgat
    49921 tgggtgggct gggcaggaaa tcccgaaagc acggcaataa tccctttgtc gcttctatcg
    49981 ccgctagcag tggttcgggt gaaaagtata ccctcggaag ccaaacggtt taggtttggg
    50041 gtaacattgg gaagcccacc aagggggcca accagctttg caccaaaact ttcaagtaga
    50101 atgataataa tatttggttg gttggtgttc aaaacatata atgtactatc ggtgtgcatg
    50161 agctcaccaa agcgttggtg ggctgtggct ttgtcaattt caacattgta tgttttactc
    50221 atgctgctgc ttttacttac ggagtacatc atgttccaaa ctgggttaag ggcagcatgg
    50281 ttgcaaaagt tattctggct aaagtacact ttaccggggt ttatgggtgc aataccaaaa
    50341 cctccccgaa ttggaattat catggttgca gccaggaaaa tgtaaattgg agagtaccac
    50401 catcttgcct tattggcttg gcttagttcc ctggcaaccc acttacggta tgcaaggtgt
    50461 gtaaggtaag tgattcctat aagcaagaca acgaatagta ttaataatgg cgtttttacc
    50521 gacgccattg cctcctttgg ggttttaagg tatagcaatg gggttgcatc gatacggtaa
    50581 ccccaattgc ggtatagttc catatcgctg ataacaaccg tatttacaac taaaagtagt
    50641 aatatggtta aagcattgta cattctagta atactctttg gggaaaccca gaaaaagata
    50701 gccaaaagca gaaatgttag tagaagaata tatcctgcaa gcgaggaatc catccaggct
    50761 cctcgaatga aaatatttat gaactcgtta aacttaaccg acttaaggtt ttcaaattgg
    50821 taaataagga aagctatacg gtaggtggaa aacagtatta cccaaaatac gaaatacctt
    50881 aaactgaatg ataaatgttt tctcatttgt gtaaatgatt taaattgcaa acctaataaa
    50941 taagaacgta taaacgtcac ggtcatgcgc aagaaaattt tagtcttgtt gtttattgtt
    51001 gggttcgttg taagcaattc attcccacaa acctggagca tcgctgttca cggtggtgct
    51061 gggaacatga aacccgaaaa ctttaccaag gagcagctcg atgagtatga acgggagatg
    51121 aaagccgctc ttggtattgg tgtaaaaatc ttgaattcgg gcggaacaag cctcgatgcc
    51181 gttgagcagg tggttcggta ccttgaggat tgcccgttgt tcaatgccgg gcggggcgca
    51241 gtttttaccc acgaggggcg taatgagctt gacgcggcaa ttatggatgg aaaaaccctt
    51301 atggctggtt cagttgctgg cgttaccgat gttaaaaatc ccatatcgct tgcgcgcaaa
    51361 atcatggagg attcccccca tgtgatgctg gcaggtaagg gggcttcgga gtttgcaaag
    51421 gtaaagggta ttgaaatcgt ggatacatca tattttttta ctcctcaacg ctggaacgac
    51481 cttcaacgta cgctatcgaa ggaaaagatg ggcactgttg gatgtgtggc gctcgatgtg
    51541 catggtaacc tggctgccgc aacatcaaca gggggaatga ctaataagcg atgggggcga
    51601 ataggcgatg cgcctatcat tggcgcagga acctacgcta acaactccac ctgcgccgtt
    51661 tcggctactg ggcatggcga atactttata agatttactg tggctcacga tatttcggca
    51721 ctcatggagt accaaagcct ttcactcaat gctgccgtag aacgtgttat catgggtaag
    51781 ctccttaatg ctggggggcg gggaggtgcg attgcggttg accgctcagg taatgtttgc
    51841 atgatgatga acaccactgg catgttccgc gcatttgccc gctccgatgg gagcagcgga
    51901 tttgctattt tttaggttac aattttgttt ttacaaaaca atttggtgta atttggaata
    51961 tatttgatga gcttatcctt atcagtaatg ttaaaccaaa tcacgtaaat atatggcact
    52021 cgaagttcat gtaaaatcag ctgaaagcaa ggggcatgtt gagatttttt tacttcactt
    52081 tgtattaatg cttatcgcca tgtttgtatt ccttgcatta ataatgctaa ttgggggttc
    52141 atttacttgg cctaattttt ggaagttcta catcacattt attcctttaa ccgtgatact
    52201 ccctgttatt gtggctatag ccacatcggt atcgtataaa agggttaatg ttcacatttt
    52261 gcctgcctca aaggttaaca ttgaaaagct caaagatttc atgtttagcg aggattatat
    52321 cattgttgat aaggataaga ctttcatacg gtttattcga agaaaaaatt taccccgatt
    52381 attaagtcta aacttcgaca agccatcaat cacaatacag aacgatgagg taatagtaaa
    52441 tgtccttaaa cgattatctt tagtattact cccacagctt accattggta aaaagtttat
    52501 aattgagccc gaaagcagta aagaatgatt aaaggttttc cttacctact catcgatatt
    52561 cctaagtgtg agcaaaacat tgccttcatg gttcaaaagg ccacgaaggc aaaagtttta
    52621 ttacgtcctc actttaaaac gcatcaaagt ttagccgttg gggaactatt ccgtaagcaa
    52681 ggggttaatc ggattacggt ttcgtcggtt gccatggctg aatactttgc tgcaggtggt
    52741 tggaacgata tcactattgc atttcctttt gtaccacaaa tggcgcagcg ggtaaatgcg
    52801 ctggcgctgg gtattgattt aaatgtaact ttctcatcac ctcaaaacct attagccaca
    52861 attgataggg ttgagagtaa agttggcgta tttgttgagg ttgatgttgg tcatagccgt
    52921 tccggagtag ctgttgacaa tacccgtgaa cttgccctta tggtgaacct tatcagttca
    52981 aaaccgaatt tggtttttaa gggttttctt actcatgccg gacactccta tagcgcgagg
    53041 ggtagggagg aagtagataa acttcattcc gaagctttaa gcaagctggc taaactccga
    53101 agcttttggg tcgaccagtt tcctgacctt accatatcgt atggcgacac tccaacctgt
    53161 tccgtatccg atgagttttg gggaattggc gagattcgtc ccggtaactt tgtgttttac
    53221 gatgtgatgc aataccaaat tggctcatgc accacttcgc aggtggctgt ggctcttatc
    53281 tgtccggtag ttgacgtgta tcccggtaag cagaaaataa ttgttcatgg tggggctatt
    53341 cacctatcaa aggacagggt tgggattgat ggagcggatt gctatggctt tgtatgcaga
    53401 tttgatggta aaggatggag caaccctatt ccaaatgcta tggtaacttc ccttagccag
    53461 gaacatgggg taatctccta ttccggtaat gacctcgatg gtataaaacc tggcgattta
    53521 cttgccattt tgccagttca cagctgtttg gcggtcgatt gcatggggca aacgttcgat
    53581 tccgaaggca atacatacga tgttttacct aaaaataaaa ggtgatgaaa aggttaactc
    53641 agtttctgtt gttcagcgta atttctacat taacaaccgc tcagtccacc aagagcaata
    53701 gtgtatttga taagtatttc gagaataaaa ccatgagggt tgattacatt cacgggggca
    53761 attcttttag cgaggatttt aaaatatttg gcataaagaa cgatggggag tggggcggaa
    53821 gagttcgggt gatggatgac ccttacatcc tggggcttta tgcatttgat ataattgatg
    53881 ctgcatcggg aaaggtttta tacacacagg gtttttgcag cgtttttggc gagtggcaaa
    53941 ccaccgctga ggctggtaaa ctaaacaaga attttgagga gagcgtcagg ttcccatggc
    54001 caaaggatat ttttaaattg gttatgcgca agcgcgatag taccaacact tttcaagtaa
    54061 tttggtctga aactattaat ccatccatgt atgccgatgc aaaccatgcc tatcctgctg
    54121 ctaaggtgca atactttgta aaaagcggta accctaagga aaaggttgat atagttgtgc
    54181 ttgccgatgg atacactgtg gctgattccg ccgaattcag gaaagatgtg gaacgtttca
    54241 ctgaggcttt cttttctgtt gaaccattta agagtcgtaa aactgatttt aacgttgctg
    54301 cggtttactc cacatcaccc actagtggca ttcgtctaca aaaacacaac tactacccgg
    54361 ccaatgtttt ggggtgcacc tattacacct ttggaactga acgttacgtt ttaaccgagc
    54421 gggagtggac tgttcgggac tttgcttcgg cagtgcccta cgattttttg gttatcctat
    54481 taaactccaa taagtatggt ggcggaggta tttacaacct ttatatcacc gcatcggcaa
    54541 aatcgagcag ttccggatat gtaatggttc acgaaatggg gcatcacata gctggccttg
    54601 ccgatgagta ctataccagc gatgtggcct accaggtttc agcccctaag tatgagccct
    54661 gggagtttaa tattacagct cttctcaatc cagcaagttt gaagtggaaa aacatggttg
    54721 aacaaaacac tccaatccca acgccctggc aaaaggaaaa ctatgagcga acaggcaaat
    54781 tgcgcattgc cgatgaagtg tactcaggta aggttggggc ttttgaggga gcaggttacc
    54841 taagtaaagg tatgtatcgc cctgaggttg actgcataat gttctcccat tcattaacat
    54901 tctgtagggt ttgccaggag ggcctaaaca gggtgctcga tatgtatgtg gttaggtaat
    54961 cctgattccc attactgtaa catcatcaac ctgaacatta tcgcctttcc attcggaaaa
    55021 tatctcaagc aaaaaatctt tttgttttgc catgggatag ctggctatgg agcacaatat
    55081 gtttctaaaa tccacaagtc taaacttttt accattgggt ccgccaaact gatcggtaaa
    55141 accatcggtg taaaggtaga taatatcatc tttttctagt tgtatgctgt gcgtgacgaa
    55201 gcttaccttc tcggttacgt aaccaattga ttgccgctcg cattttacct ggtatagggt
    55261 gtgtgtctcg ttttggttca aaataatatt tgagactttt tgactattcg atttccgaac
    55321 aatgttaagg taaatattag cgccggagta ccttaattcc cttgtttcag ggttgtagtt
    55381 gcaaaaggca atatccattc catcaaaaat ttcggagcct tccctttgct caaacgattt
    55441 atggaataat tcgttcaaat tttgcagtac ctcgcctggg ttggtctcat taagttcgct
    55501 aacaatttgg ttaagcccaa acatgccaat aatactcatg aatgctccgg gtgccccatg
    55561 gcctgtacaa tcggcaactg caaaaagcat ttgattgtta aaacgcttaa accagtaaaa
    55621 gtcaccacta acaagttcct taggcaggaa aatcagaaag gaatcgtcga aaaaatctgt
    55681 tggctgttct gaggtttgta aaatcgcttt ttgaatcctg cgagcatagt taaggctatc
    55741 ggttatgtcg cgggagcgtt gttcaagcat gtttttctgc ttctcaattt tttccttttg
    55801 ctccgcaagc aagaggtttg ctttttgctt gtttctgaat gccaaatacg aaactactac
    55861 cagtacaatt agtattgcaa taaaaccaac cagaaaaagc tgacgcaatc gcgatttgtt
    55921 caagttggct tggcttaata aaagattctc tcgttggcgt tgaatttcct tctctttttg
    55981 acgtaaggct gatatgtttt ccagtctatt ggcttcgcta ataatttgat tgttaaatag
    56041 gctgtcagtt aatgaaatgt atttcctgag gtactggtaa cctaaggttt tgttcccctg
    56101 taactcttcc agttgtgaga gttccttgta tgcaagtcgg ctaatgttag ttatactcaa
    56161 actttccgat tgctttaggc attgaagtag atactttttt gattcggaat agtttccaac
    56221 tctggcgtgt gccttaccca aaagcagtaa agggtaaagt tgcccgtacc ggtcgtttat
    56281 ttcattttga gtatttagga ttgactgtaa aagcggaatc gcactatttg gttttagctg
    56341 attaattaat agttcagcac caacagtttg agttctggct aactccttgg ggttgttttt
    56401 taggtaggtt tccgattttt ttaaatgttt atcggccatc agattgtttg attgtctcaa
    56461 gctattgttg gccagcgctc tgtatgttat acctaaatat gtgctatcca ctaccacttt
    56521 caggatggct atggcatttt caaggtagct ttgggctgta acaaaatttc caaactctgt
    56581 gtgaataatt ccttgcgatg tataaatttt tgctaggctt aatgaatcgt tgcgggattt
    56641 aaagaagtta atagcagtaa ggttgttctc tatggcgttg ttcaagcggc caagttctcc
    56701 ttccactttt gagattgata gcaagtacca gccaattttt aacgaatccc ttgtggcaat
    56761 gctgtagcgg taaccagggt atagataatc aatcgattcg taaagcaacc ctttttcgat
    56821 atggatttgc gaaaggaggt agtatgcctt ttccaggtac tgttcgcgtc ccatatccaa
    56881 tgagactatt aacgcctggt tagcttcaat tagggcattg tctaaattct tcttattaaa
    56941 aaggtattgc gattttgcaa ttaacgtctc cacaagtttt accttatcgc cattcctctc
    57001 atactgacgg gttagatttt caaaatactt taaggaatca atattatcta atggctctgg
    57061 tttggcattc acccttagtg ttggtatgcc aaatacaact aaaagcagaa atgctttgaa
    57121 ggtgataggt ttaaacactt gttaaaacat taaagttatg gtataaatgt aaaaaatatt
    57181 tcaacatcaa agtcttgaaa attgactaaa acagttcgct aagcaggtca agcaattcat
    57241 tcaccttaat gggtttagtc aagtagccat aagcacctcg ggcaatagct ttgtcaatat
    57301 attcagctgg ggtatatgcc gattgaacta aaatgggaac attgggtatt ttttctttaa
    57361 ttctactgat aagttctagt ccattggtgt cgggaagttg aatgtcgagt aatacagcat
    57421 ctatcttttc ccggttcaat aaatcaatgg cctgagaacc cgacattgcg gtaaggcttt
    57481 ttgcgcctga ggcaattact acatcgctca ggtagttaac gttggcttcg ttatcttcaa
    57541 caatcagtat ggttttgccc ttaattttgg tatagtcgtt tttggtaata aaatttatac
    57601 ggctaatgtt tttggtatta gggattataa ggggtagggt aaagctgaaa cgcgagccct
    57661 tattgggttc tgattcaacc caaatccttc cacccattag ctctaccaaa cgttttgata
    57721 ttgccaatcc taagcctgcc ccgccataga ggcgctgagc cgtttcgtcg gcctgtcgga
    57781 atctctcaaa tattttttca tgcagtgatt tagggatgcc tataccggta tcgctcacaa
    57841 aaaactcaat ggtggcattg tttggaatac ggtagcccat ggtaacgcta ccctgcgagg
    57901 taaacttaat ggcattgcta agcaggttaa gcagaacctg cttaagccgt tcaccatcgg
    57961 caagtatgtg tgttattcca ctgttgttac cgatatcgcg tgctattaaa atattctgtt
    58021 tttgtctttt tttctcgtta atggcaaatt ggtgaagctc gttgaacagg ctatcaagct
    58081 ccacgggttg ccttcgtatg gagtatattc ctgcttcaat cttggagagt tcaataatgt
    58141 catcgataag ggttaggagt agccgcccat tactggtaat gtagtcggca taaatcttct
    58201 ttttctcagg gcttatggtt tcgcgggtaa ttaattccga gaaaccgagt atggcattca
    58261 tgggggttcg aatttcgtgg ctcatattgg ccaaaaaggc cgattttagc ttgtcggctt
    58321 cttcagcttt tttcttggct agcatcaact cgttttcccg ttctttttgt attatcatgt
    58381 aggttagctg cgatgaaaca aactcaagaa ctgccaggtc gttatggtcg taagcatttg
    58441 ggttttggta gttttgcaca gcaagcaccc ctatggtatc gcttttagtt ttaagcggca
    58501 cagccatcca gcacaatgcc ggggggccgt aaaaagtaag attatgcttg cttctaagcc
    58561 tgttcatctg ttgccggttc agaataacac cttgaccgcg atgcgctacc cacagggcaa
    58621 aggtgtttcc gcttggataa tcggtgttta tggggtgggt gtcactaaca taggctaagc
    58681 ttagcctttg tcgactctta tcgaacagca caaggtagat attcatggca tcgaaaagcg
    58741 tgttcagctc ttcctgcatt aacctgtaaa aatcctcagg cgaagtggtt tcctgtccta
    58801 ttgcggatag ttttaccagt attcggttta tccgttcaac cttattgctt aaggttatat
    58861 tttcaatcca gactaaaaaa atgttccatt ccacaggttt aacatttgcc ctgtaggtgt
    58921 gctgtttgcc gtgtgtgctg tgttcaaatg tgtatgtacg ctctttcttc gatgttttaa
    58981 agggtaaatt ggggaatagg ctttcaattt tacatccatc cggaataggt ttatccgtaa
    59041 gtaaaagtac tgatgggcta tagtttaaaa ctgttccatc cctttttacc agcagaacgg
    59101 ctataggcaa atcatttata tctatgtgat ttttattgtt ttttgtcatt atttttttcc
    59161 agcgtttttg gatgcaagca tcaaaattaa ctgtctaatt taggaaattt cctttaatcc
    59221 actatacagt gttaaatgtg tttaatttta gatgggcttc tacgtgttga aatgttgtat
    59281 ctaattgagc ttagctgttg caatttatgt gtaaatggta tttttgcatt aaattttgtt
    59341 ttatatgcat aaaatactat atgttctgtt atttgcaatt atttccattt catgtggcga
    59401 aggtggcaaa ccgagcagca attcaatcca tgggaatggc tatgcaaagc atgcaaaagg
    59461 ttttgatata gagatagatg gctcagtgac taaagtatgg gtcaacaatc cgtttgaacc
    59521 tactgccaat ggcttggttt atacactgta tgccgattca atgcgaagca tagacccgaa
    59581 tagtattcaa attcctgtaa aaagggttgt ttgcatgtcg tccacccatg taggttttat
    59641 aggcgcttta ggtgccaatt ctgctattgt tggaatttcg ggaacaaagt atgtcaataa
    59701 tcctaaactc tgggaacggg ttgttaatgg acaaattgcc gaggtaggtt acgaccaaac
    59761 cttgaactac gagttgttaa cccgcttgaa gcccgatgta atttttgttt acggggttag
    59821 ttcggaatcg ctatcgttta tcgataagct aaaggagttg gggttaaaga tggtaatggt
    59881 gtccgattac ctagaaacat cgccattagg cagggctgag tggattaagt tttttggagt
    59941 tttttttggt aaaaaggagt tggccgattc aattttcagt gaggttgagg taaactatgc
    60001 caagctaacc cagtcagtgg aacaggttga tactaagcca aaggtttttc ttaatttgcc
    60061 attcagggat atctggtact tcccgggagt tgataattac tttgtgaagc tgattaacga
    60121 tgctggcgga agttacattt tcccagagct tacaggctcc caaagccatc cggtgagcac
    60181 ggaggtagcg tacaaggctg gtttaagctg cgatatttgg ttaaaccctg gcaccgctaa
    60241 cagccttagc gatattacca attccgataa tcgtttttcc atgtttaaat cgtttggcaa
    60301 tgcaagggtt tacaacaaca ataaaaggat ggggcaggga ggcggcaacg atttttggga
    60361 atcgggggtg gtgcatcctg agcaaatact aagcgatatg ataaaaatat ttcatcctga
    60421 attaatgccc accgatacat tgtactttta caggcgattg gagtaaccat atctttaaaa
    60481 aaaaaacaat tacttatgaa atttggagtc ataattgaaa ctaaggaacc cgaaaaggca
    60541 tggaatgcct ttaggtttgc tgtggcatca ctgggtaagg ggcacgaggt taaggtgttt
    60601 ttaatgggtg aggctgttga gtgtcagggt ttaacccata acaaatttga tgttgatgca
    60661 caaatgcgca agttcacagc tgagggcgga acaatcttgg cttgcggaac ctgccttaag
    60721 tcgcgccaga tggatggaac cgagctatgt cccatatcaa ccatggccga ttgtattcaa
    60781 atggtggaat gggccgatag ggttatcacg ttttaaccaa tacacacaaa gcaatgcagg
    60841 gtagaacccg atatacaata ctatttatct cgctgatggt ggttttattg ctattagccc
    60901 tgcttagctt gcttttgggt tcagtggcaa taccactggc cgatatcaag gcctattttt
    60961 tgggtggaag ctttaccgat gaggtgaatt tatccataat tcaaaatttc cgaatgccaa
    61021 aggtggtttc cgctttgctt gccggttccg ctttggcagt aagcgggtta caaatgcaaa
    61081 ccgttttccg gaatccgttg gccggcccat atgttttggg catcagctcc ggtgctagtt
    61141 taggagttgc gcttgtgcta atgggttctt cggcatttgg gctttcgttt tggggcagtc
    61201 aggtaggaac ggttgtggca gcgcttgttg gttcgctttt ggttttaatg cttatactgg
    61261 cagtgtccat tcgggtaaga gatattatga cactgttgat tttagggatg atgtttggca
    61321 cggctgtttc agcagtaaca agtcttttgc agtactttgg tagcgaaact tccttaaaga
    61381 attttgtgat atggacgatg ggtagtttag gaggtgttgg ccgtcagcag ctttggcttt
    61441 atgctgcgct ggttctttta ggacttgcac ttgcctgggc gtacgttcgt ccgttaaatg
    61501 tacttggaat aggcgacacc tatgcgcgta gccttggttt caatgtaaag caaacgcggt
    61561 tcctgatatt cctttcaaca agcttgttgg ccggagcggc taccgctttc aatgggccta
    61621 tagcttttgt gggcatagcc gtgccgcact tggcgaggat ggtttttcga acttcaaacc
    61681 atgccattct ggtgcctgcg tcggcattgg caggtgctgc tactctttta ctttgcgata
    61741 ttattgctca gctaccaggg tcggcaactg tgcttccctt aaactcggtg gcctcgctaa
    61801 tgggtatccc tgttgttatt tatatcgttc taaaaaacaa acgcgtagtt agctgatgaa
    61861 cggtatagtg attgacataa aaggattagt atccggttac cgaaatggag tttcatcaac
    61921 atataggtat ccagcgttta acgccgtggc tttgcgtggc gaacttattg cgcttattgg
    61981 acggaatggg gtgggcaaga gcacgctgct tagaacccta gcacgcatac agccatcgct
    62041 tgatgggaat gtttttgtta aaagggttaa ctttcagcag atatcaggtt cagagtttgc
    62101 acagctggtg agttttatcc cctcggagcc agcacatgct tcgcatacta cggtttacga
    62161 gtttgttagc atggcccgtt atccttacca tggttggttt gaggctttaa atgataatga
    62221 ccataaattg gttgatgatg caatatcggc cgttggcatg cagaattttc gggatcggtt
    62281 tatcgataac ctgagcgatg gagagcgcca gcgcgtaatg attgcttttg caattgcaca
    62341 ggatacgcca attatcatga tggatgagcc aacagctttt cttgatttgc ccaataagtt
    62401 tgaggttatg cgtttgctaa gggagcaagc acaaaagggt aaaactatta tcatttccac
    62461 tcacgacctg caaaccgctt ttggtttagt tgataccatt tggcttatgc tttccgatgg
    62521 tgttagggtt ggcgctccgg aggatattgt tctgtccggt aacctcaacc gcttaatgga
    62581 cgatacgcct gtacttttcg atttgaaatc gggacagttt gtctataagc atcaaaccag
    62641 gttagtggct tgtgttttgg gcggttcgcc caatataaaa aagtggacct cgcacgcact
    62701 aaggcgtatg ggatacgagg ttgaattctc ccccattgac gatgctagta cctacgttca
    62761 aattcaggag catgagggta aggtacaatg gatacttggc cgcgacgacc gtaatcctac
    62821 cttcgattcc attcggaatc tatgtatcta cattaaggga accgcgcttc tatagcttta
    62881 tttcaaagct atcggcatct aagtgaacgg ggaatgaact tcttacgcta tgtaggtaat
    62941 ccttatcgat gcttgaataa attacttcct ccgtgtttgg tttagcctgg gcaataattt
    63001 gacccatggg gtcaatgatt tgcgagtcac cggtgtggtt tatttggttg ccatcggttc
    63061 caacacggtt gcaggcagcc acgtaaacct gattttcgat ggctcttgcc accagtagcg
    63121 agttccaggc atacctacga cgctcaggga aatttgcaac aagtatcatc aaatcgtaat
    63181 cgttccagtt acggagccaa accgggaaac gcaggtcgta gcatactgcc agcagaattc
    63241 gccagccctc atgggttaca atcacgcgtt gtgttcctga tgtatagtgt gcgtcctcgt
    63301 ttgccattcg gaaaaggtgg cgtttatcgt aggtttcaat tgtgccgtta gggtaggcaa
    63361 aaaccatacg gttgaagtac ttgccatcag tttcggcaat gtagctgccc gcaagggcaa
    63421 acccatgctt ttttgccatg gatttcatcc attcaacggt tcgtcccgga tgcctctcag
    63481 caagggaggc tggactcatg ctgaagccgg ttgtgaacat ctcgggaagt aaaaccaggt
    63541 gtgttccggg ttcaatccca gccagtttct gctcaaactg ctcaaagtta ttatcgggca
    63601 attcccagta tagtttactt tgaattactg ctatcttcat tttccgttct ccaattaatg
    63661 atgcactgtt caacaagtgt ctcgtaatgg gccgagatat ctcttaccaa atcaggtatt
    63721 tcgtggtaaa tgtgattgtc aatctgctta accggtaaaa cactgggaga ggtgcccgtg
    63781 ataaagcatc cggtggtttg cttaatttca ttagccccaa gactcctaaa gttaatgttg
    63841 atttgcaggg tgttgcatat ttcaatcacc ttttgacgcg ttattccacc aagcactttg
    63901 ctattggggg cagtaattag ttcattatct ttaataaaaa agacgtttga tcgactcccc
    63961 tcagtgataa agccatcgtg gtcaaccaga agaacctcgt agcaccggtg tgtttctaaa
    64021 atttcgttgg caatggccct aatggctttg ttctcaaact taacagttgg gttatgcctt
    64081 tcggcattga gggtttcaag gcgaaccccc gtgcttatct catcggcggt tgggtagcgc
    64141 gatggtataa agtatatcaa tagggtagct ccgttttcat tggaataata catcaaccta
    64201 aaatttctct gatcaaccgg gtttgcttta agtagttccc taattccaag ggtgatctca
    64261 tcaagattta tattatagcc catcatctgg gcagagtaca taaaacggtt taggtgatcg
    64321 ttcagaaata ttggagtgga gttttttaca cgaattactt cgtaaataat attttcaccc
    64381 tcggttaggg cgacggaaaa cttattgagg ggtaatattt ttccgttata aaagaaaaaa
    64441 ctttgttgat tatcgggagg aaaattcatg ccataaaggt ttccagcaaa aatagcacaa
    64501 gctatttttt ttaaagcaat atcctaatca aatgtgggtt ttggttttaa acttactgtt
    64561 cccttgatgt ataaatgtct aatatattgt tgattagggg taaataaaaa ttgttgaaga
    64621 aagcattcct tggtgagttt aggtgaagaa tagagtttag cgttttcccc cctttcagct
    64681 ttgatgtggc tatgctaacg ctgttttcgg caaaatcgcc tgcaaagtag tagcagaact
    64741 tatcggaata ccctttcacc acagctggga actggtttgt taggccaaac gattttaatt
    64801 tttcctcgcc tgctggggtt gtttccagct taaaagtggc atgtacctct gaaccgattc
    64861 cagccatgat aatatcgaac catccgtaaa attcagtttc ttttgaattg ttttgccatt
    64921 gaggcatggt aggttcggca atgagtttaa ctctggggtt tagaaggtca gagccttcct
    64981 caagcacatc aattaaccca tcgttgctaa tcaggataat gcctgagcct ttacttggcc
    65041 aaggtttctg gtgttgtttc tcgtatagct taaccatcca ttcggctggt cccttaccgt
    65101 tgttaattgc cccaagattt ggacaatatt taccagccca accagtccag cttacattga
    65161 aaagttcttc ggttttggtt cggataagtg catttgtggg ggagctaaac atgttgtact
    65221 cgccaataat aagcttattg agttccttca tccgtttgag gagcaagtag tcgttctggt
    65281 ttagacctcc gtaaaccttt gatgtgtttg gggctttggt ttcggtttta taccactcgt
    65341 aggagtaaac tccatagcag tcggtatagt aggcggcatc gtaaacggca gcataggcat
    65401 ctacctcgtt cagtcgaatc gattttatat cgaatagttg ctgatttaag tcgatgggga
    65461 aaaagcccca gtaatccgat ttatggttgt acgatttgcc atcggggccc acaatccgaa
    65521 agtggttgag cagccagaca agcgatttgt gctcaattct atcggtttgg gttactgttt
    65581 tgtcaagtat atatatgttg agtggctttg acttctgaaa gtaccaggct aggtgaagcg
    65641 caataacaat accaatgata catgcaatag caattccaag gtaagtggca atctttttca
    65701 ttgcaattag gtttctaaaa cttgtttagc cttggcaaat tacaaaaaaa tgaaaaagat
    65761 tacaagtgaa ggggataaat tcgtgaaacg tgaatcgtta accgttaacc gtgaaccgtt
    65821 aaaagttaaa cgtgaaaagt taaaagttaa aagcttggct ttaagtggga atgagtcgga
    65881 atactcggaa tgagtcggaa tactcggaat gagtcggaat actcggaata tgatggagtt
    65941 agatgaagtt agatgaagtt agaggtagtt tgagttcgtg aaaagttaaa agttaaaagg
    66001 ttaacgcatg gctttaagtg ggaatggttc ggaatgagtc ggaatgctcg gaaagagtcg
    66061 gaatgagtcg gaatacccgg aatgtttcgt aatagacact cttttacata caaccgtcaa
    66121 ccgtcaaccg tcaaccatca accgtcaact gtctacccaa ttagtttcac agtgttacac
    66181 ggagtttaac acagtgttac aaagtgaaaa tccatcatat ctgcggaatc agtgttcaat
    66241 ttccaaacac aaaacacaaa acacaaagca ctaaatacta aaaacatcaa ccgtcaacca
    66301 tcaaccgtca accatcaact gtcaactatc aaccatcaac catcaactta attattggct
    66361 tgagcctttt gggattcagc caggagatga tttgatggca tatttactaa aagattttta
    66421 ccactaaaac ccttgcagtg tttgttgatt tattggtaag tgaacgactt tcgttaatct
    66481 caacaaacgt agctgaattt ggggtaagta aagccgattc gttattcacg gtgagttcaa
    66541 gttcaccctc aagtaggtag aatacaacac caaatgggtt tgcgtgcgcg ggtattacct
    66601 cgcctggttt tagcgttaga tgaaccattt ctactttaga acttgaaaac attatacgcc
    66661 cgtccaaatt gaatggaacc tttggcgcac tttgtagggt ttcgggttta atcatttttt
    66721 caaattttta accgccatat acatgtatac tttgacttgc agagggcaag tattttattc
    66781 ccagccaggt aattagcaga acccatagtg caacaatcat taaacgagcc agcagcttgt
    66841 gtttggtagg catgtaccgg tggatatgta taactagaag atagaaaaac caggtaatta
    66901 atgcccaaac ctcctttggg tcccatgccc agtagtcgcc ccatgcaaac ttagcccagt
    66961 aagcgcctag gagcattccc agcgttaaaa gcgcaaaccc gggatatact agccgcattt
    67021 gctgctggag ttcatcgtga actgaacctt ttgaaaatga tgtccaagcc actgaaaggc
    67081 ttgctgatgc aagaactgca taggctagca tatacgaaac cacatgtggt ataaaccagg
    67141 ggctttgaag agcaggcata agaaacctac cctgatattc tggatgaatg atatcaacaa
    67201 ccagaaaaac cgaggccatg attaggccta tggttagaat gtttttactg cgatatgctt
    67261 taaatacgct taggctaaca aggcttacca agaaagagta ccatattcta gtctcagcca
    67321 tggttcgcat gggcggacgt ttaatatcga accagtataa gataataaat acccccagaa
    67381 gagcactggc tgtaacacca gaataccata aaatgagttt accttttgcg gattctttgt
    67441 ggttaaagta aacagaaact aaccaaagta atatcacagt cagagcaacg ggaacaaaat
    67501 aattccaaac catttgttta cttctttgaa tcgttaatta aaaccaagcc taatccaccc
    67561 aggagcatca tggcaaatcc tatgtatatc agtggtaacc atgggtcgta gattacctta
    67621 aaggagctgt aggttgagtc tgggccagcc agattatcgt atgaatactg gtaaatccac
    67681 caaccatcaa ccctaaaagg tttgtttacc tcaataaggc attggtttac ggaacttgat
    67741 ttttttgaat agattttggc gtatgaacca aagtattttg cctcggcagg taaaagtctt
    67801 agctctctgc catcgggaag tggaagggtc ttttcggctg taacatgggt aaggctcgag
    67861 atccagccca ttgtaacagt ttgcccattc gagtcaaata cttgtatcat tgccgctggc
    67921 ccggtaaagg gaacaccccg cgcatcaatg aaggttgaat cggttgcaaa tgccttgggt
    67981 aagtattgca taacctgaat ggttaggtta tcaattttaa tgcttgggtt actgtttagg
    68041 tcgtattccg attctgggaa gaatgtctta ctacccatta aagccaatcg aggttggtag
    68101 aattgtgctt caaacttttg tagttcaatt gctattggca attcaattac actaccgttc
    68161 ttgtctgttc cgtaccatgc aatttcattc tggttaacct gaacggttgc ctcaatctta
    68221 tcggaacttc ctactaaacc tgccgataag caaacccaaa gcccggcatg gttaagggtg
    68281 aaaattatgt tttttagggt aattggaaat acacggttta caacagccgc ccctaaggtt
    68341 attagcataa aaattaaagg gaataaaaac aaccaggaac ccagtaggtt aaataatgcc
    68401 ccgggtattc ccataccatg tcctatctgt gggataattc ccattagaat aattgagaag
    68461 agaaataatg aaattgatgg aatagctgac ttaaccgagg caagccagtt gaaaaatttc
    68521 gattttggga aaatgatttt taagaggatt aaaaggctaa gaattgtaac taaaacgata
    68581 gcattggttg ggaaacctag tttaggaaat agcgatagag ggacaaatag ttccaaaaag
    68641 aaagctagta cccataaccc aacggacaca ataaaaccct ctaaaaaacc ccaaggccat
    68701 agccaaaggg gtttcttttt aaattgattg cccatgattt tgattgctac attattcttt
    68761 caggcaaagg catgttctgt tcacgctctt ttgctaattg taaccacttg ggaagcacat
    68821 ttttcttcca ttcttctttc tcgcccttaa gttttttcag atcaattccc acataggctt
    68881 gagctttttc ctttgatgaa aggtctggca tgggaacagg ctcattgtgt cctaacgttg
    68941 caagtaccct ggagagctgt aagcgcgctt ctgaggcttt ctctattcca tcggcaatta
    69001 ttcgcatact ctcaagagga gcatggaacg atgctccatg gcttgcggct acaaaatccc
    69061 aacgccactg tgcttgtctg ataagtttca atactggctc catcattttt tcattggcgc
    69121 cattgtccca tgcgaatttt gcttctatat gtgcctttac caataagtct tccagtacaa
    69181 ttttttgggt atatactctg tcctgcctat cgtaaacgtt cttaatgagt tcttccttgg
    69241 tttgtttatg acaaaccata catgaggcct ccatgttgtt cagggggctt tgaaccttat
    69301 ggtcggtaat tttctggcta ccttctgtaa cgtatggcat atgacaatcg gcacatgaaa
    69361 ccccgcgctg gtagtgaatg gaatgttgga aaagctcaaa atcggggtgc tgagccttta
    69421 tgattggagt tttgcttaag gggtgaatcc agtcggcaaa tgcgtattcg tcgtagtact
    69481 tctcaatgtc atcaacgtta agccctttat cccaagggaa ggttacatat ttaccatcgc
    69541 ccttaaagta gtactccaca tggcactggg cgcacaccat ggtgcgcatt ttatttagcg
    69601 gcaacaactc aacgttacta cccatacgct cataagcttc ttttaatggg atttggaaca
    69661 ggcgtagagt catatcgctt ggattgtggc agtttgcaca accaataggg tttacaattt
    69721 ccgagagcat agccgaccac tttgttttgt agaaatcggc tggcgacatt ttagccataa
    69781 cccttgcaac atcggggctc ttgcaagccc agcaacttga attttgaggg ccctcattct
    69841 cattggcagg tgtgccagtg cgtaacgtat tatgaacatc cgccactgag tatacatgac
    69901 ctcgggcctg attaaaatct tttgaaaagg catagccggc aaagagtatt accagtcttg
    69961 ggtcttcgtc aagtaaatcg cgaaaagcgc ttccgccata ttttgaccgg aaaagggtat
    70021 cggacatctg ggagtaggtt tgatactctc gcggaaatgc cataccccat gcctcgtttc
    70081 ggggctcaaa ctcatcgatc ttatacctag gagtattcat taggtttgct tctgttcgtc
    70141 gctgaacaat gctggctgcc agtaaaccaa gaacaaaaac aacaatcatt gttcccagaa
    70201 ataatgccca tccaatccat ggattcttgg caattttttc acttaacgga gttttcatgt
    70261 tggtataggt ttaatatcta tttaatcagt ttctttaacc agttaggggt gtcagtttta
    70321 ggcactggtg cagtgccata tggtgttgcg gaaaggcttt ttactgtgcc atggggtacc
    70381 tcacggtgac aatcccagca acgtttccca tttccggcaa gaattgcatc taaatcgaca
    70441 ttggctttaa ccatggctgt gaggtttcca tggcagcgta tgcagttttc ctgaaccact
    70501 ctttgcccag gcttatgcat gtaaatggtt tgggggtaag tgtttagtgt aaagatggtg
    70561 gcatgccgca aaccatcgtt tgctttaaag taatacttac ggacgatgtt gtcgtggggt
    70621 acgtgacaat cgttgcaggt ggcgtgttcc ctatgcgatg agtggtgcca ggtaacatat
    70681 tgcgtcctca tgatatggca attgacgcac gttgcagggt cgtcggacac atacgaccat
    70741 gccctggagg tataaaaaac aaacaatcct gttccagtaa aaattcccgc caatataatt
    70801 actggtactc gccagtttgg tgggggttga aaacgtttta gtatctgttt agcccagtca
    70861 aacatattga tgttttttca aatttaggta ataagaagct tagaatcaat ttaaatattt
    70921 gcgcttatac cacatcataa agcccgatag tactactacc acaaggaaaa tgcctgttaa
    70981 tggaaccacc agtatgtaaa agtcgccaag cataaagctg aaaattcgac cggtatggat
    71041 ttcctgagcc agattccaca gcgggaattt gaaagcatca gcaactgttg atggcattgg
    71101 aatagcaggc tctgaatgat gtaaagcaac catcccttta tcgtaatcga atatgtattc
    71161 taaccctgaa atatgagcgt atcccgatac tacattctcg cctaatggtt ttcgtaggcc
    71221 gttttctctt ctatgcaggt tgcctgttat gtagttgaag gtttctcccg tgtcggggtt
    71281 ccagcggaat aatccgctaa agcttcccac caggtaaaac acgctgtcta gaggctcaag
    71341 aacattgata cccattacgc taactggtgg ctgattggga aatttggtta agggctctga
    71401 gaagtcagtg caatggtaaa ttccatcgga cgttgccagg atgaactttt ttctagctga
    71461 gtcaaatctt atatcgcgaa gtttatcgtt ccagtagtag ctgttagaaa ccttttggtt
    71521 gttaagcact atttgactat tggcaatggt tagcaggaaa ggaggacgca ggaaagttcc
    71581 tgttaggccc gatattacca atagcaatgt agccagtgct cctaccttta agtgccattt
    71641 ataagagaaa cgggtggtgc gttttagttt atggaacctg aattgctcat ggtacctttt
    71701 gattattttg ggggataaaa agtagataat acctgttatg cttaagaaaa tcatgactaa
    71761 agcaaccaaa tcggctaata atcgtccggg aattccaaaa atctcgccgc tatgaatatt
    71821 ccaaaaaagc cggaaggttg atatttcctt tttggaacct agaggggctt tcaaatcgat
    71881 tggcgtaaac tgcagggttt gtgccgatga ataataaatt gaattacgtg ttgtaatgta
    71941 tatacttgat tcgaccacct caattgaggt gcagaattca tcgctttggg gtaggtgcaa
    72001 ctttacccaa tgattttccc ctttgccaag ccagtacaaa ccgaaacggg tggcagcaag
    72061 tatcccgttt ggggttactt caaggtcaaa aatctttctg taatcgctcc cttccgggat
    72121 accatccatt aagggcttcc aattttcaaa attgcttttg gtaagccaaa tcccaactcc
    72181 cccataaagg atgatagaat cagagttaat ggttcttgct ccttttaccg atgccaggtt
    72241 ccagtttttt aactggtagt tcttaggtaa atacttacgg ttgatatcga tatgggcaat
    72301 ggtttgccgg tgattcatca aaactcccga aatggcaaaa agcaaaaaaa acaatgtaaa
    72361 tactaaagcg ggccatttgt ggtacttccg cagagtacta taaaaactca taatagcaaa
    72421 aggctttttc ttacagaaaa atgtttacgg tttatagaaa tagatgttaa agtgttcatc
    72481 gtcaatcttt tctacccagt gttttattcc caagctcgaa gccttatcta ttaagggtgc
    72541 agtaataaat ggggcaacca ctttgtatat tttatcagaa ctcaggtcgg ttaaatcggc
    72601 cattatctgg ttaactggtt gctcaccagc ctgtaacatc acccttacat caatttctga
    72661 gtcaactttt ttagaatcga accattgggg tttctgggtg gtgaactcca tctcatcggc
    72721 accctcaagc aagtcctgtc ctacagcttt ccggaggaca ttaatgagtt cctctacctt
    72781 aacatttcct attgctgcag cttgctgaag tgttgcaaca cgagcaactg ttttccttag
    72841 aacaggattt ttcagctttt caaaagctgg aacataccca ataagcacct cctcgagctg
    72901 cgggtatgct tcaattagct gcaaaacctt ggttttaggt gttataatga gtttgttttc
    72961 gtccatagct atttggaata tgatagaata cgttgttcac cttcaagttt gcgatacact
    73021 gaaacattgt gcgaaacctc caaaacacca aggtattcgc ctttctcgtt tcgtaatgca
    73081 aagtactcaa tatgtattag gttgccacca atattaatcc agaagggggc tctagaagcc
    73141 tttccgcttt taaagtcctc aatgatttta tcaacaatgt gagcactggc agggggatga
    73201 cataatctta catcgcggtt aaggatagcc ctgttacgct ggaatattct ttcggggctt
    73261 tgcgagaaat acttaacctt atcgttttta tcaacaaatg tgatatcaac tggtaatgtg
    73321 ttaaggattg ccagaagttc ttccactgag aaactacccg ttgggagttg aatgttttcg
    73381 gccgttttgt ttaactcctg aagctcgctg ccttcaaccc atgatggttt ccattttgta
    73441 ggagggtcgt ataggcaaaa gcctatctca agctgctgct tatgaatctc gtaccagtca
    73501 gcttcagtaa gcttatccaa ggccatgggg aaaagaattt cttcctcctt ggtagtcatt
    73561 tccataacgc ctttaatggc agggcggagc acaatctcgc ttgaggcaat tagttcgtcg
    73621 cgtgatatcc cttcggtttg caggagttca attgagcctt taataagctc tcttatttca
    73681 tcgtgttttc cccacattac ctttggaggg ccagtaattc cctgcttttc aagaaagggg
    73741 aaaagtaggt attcctttcg ctgatagtgc ttatctacat caaacagagc gttaaactgt
    73801 cccctaagct tgagtacaag ctttgggatg tcaactgctt cacttgacac aagttccata
    73861 agcgattgct caatggattg gcaaagctgg ttgagttctt tattttcctt aatgaacaca
    73921 tcaacagggt gaccgtcggg aattttttta accgttgtaa gatcaatatt tcccttcagc
    73981 actgctgagt gtacatcgca aagtttcagg acttcctgtt ccggcaaacc ttcgctaatc
    74041 aattcctgct caacctcaac tacctcgcca taggggatat tggtcaggct ttgtattagc
    74101 tcctgccgca cttcctgttc tgagtcgcct ttatgtaacc ggagaatgag ctctttgagc
    74161 tttttcttcc tgttttcgga gttattaatc agttcgctca tgttttttct tttaaagtta
    74221 ggaataaaat aagcgcaagg caagggaatt gattaaaatc aactattcct ttatcatggt
    74281 taaaaccatt ttaaaccttt cctcagcctg aagcgcatgg ggtatgtttg caggcatgat
    74341 aattgtttcg cctgcaacaa gttggtgggg tgttccaccg attattattg tggctttacc
    74401 ttcaataacc tgaacaacgg catcaaaagg tgctgtgtgc tcgcttagac cctcaccctt
    74461 atcgaatgca aaaagcgaga tgttccccgt tgggcgtttt agcacgtttt tgcttactat
    74521 gccgccattg ctgtattcaa cctcattcat aaagttgaac ttttgtcctt taggaaattc
    74581 attcattaag ttcatagcaa tttggattag agttaattta tgcacaaatg aaaaatccca
    74641 gccgtaatgc atacggctag gaaaaagggc tagcctacca ttagcttgat gtcttcatct
    74701 acagtagtga ttccaccaat tccaaagttc tcaaccaata ctttggccac attgggagag
    74761 aggaatgcag gaagtgttgg gcctaggtga atgttcttta ctcccaggta gagtagcgca
    74821 agaagaacaa ttacagcctt ttgttcatac catgcaatgt tgtaggcaat tggcagttca
    74881 ttgatatcgt taagttggaa tacctctttt agtttaagag cgataactgc caatgaatag
    74941 gaatcgttgc attggccggc atcaagtaca cgaggaattc cacctatatc acctaaaggt
    75001 aatttgttat agcgatattt tgcgcaacct gcagtaagga taacagtatc ctttggtagt
    75061 ttctcagcaa actcggtgta gtagttgcga tccttcatac ggccatcgca tccggccatt
    75121 acgataaact tacgaatagc tcccgatttt acagcatcta ctaccttgtc ggccaaggca
    75181 aacacctgtt cgtgagcaaa tccgcctata atttcgccct tctctatctc aacaggaggt
    75241 ttgcaacgct ttgcgtgttc aatgattgag ctgaaatcct ttggttttcc attctccctg
    75301 tcggggatgt gttttgcgcc ctcaagacct gctgcgccgg ttgtgtaaat cctatcctta
    75361 tagctggcgt tggcaagtgg tggaacaata cagttagtgg tgaacaggat tgggccattg
    75421 aacgactcaa aatcctcgcg ctggtgccac cagctgcttc cgtagttacc cacaaagtgt
    75481 ttgtatttct taaaagcagg gtagtagttg gcgggtagca tctcgctatg ggtgtaaaca
    75541 tcaaccccag tgccctcggt ttgggcaagg agttcctcca tgtccttaag atcgtgacca
    75601 ctgattagga ttcccggatt gttacgaacg ccaaggttta ccttggtgat ttcagggtta
    75661 ccgaagttgg tggtgttggc cttatcgagc agcgccatgg tttgcacgcc atacttcccg
    75721 gtttcaaggg tgagtgcaac caactcatcg gcattcaaat tctttgtgat atcggccatg
    75781 gccttttgca tgaatgcata aacagtggtg tcctcaaagc caaggttgta ggcatgttcg
    75841 gcataggccg ccataccctt tacgccgtag gtaatcagct ccttaaggga acggatatcc
    75901 tcgttggttt cgttcagaac tcccacggtg gcagctttag catcaaactc ttcgggctta
    75961 ccttcccatg tgcatccctc aaaggtggtg tcaaccttta cccctttctt ttgggcttcc
    76021 tcgcgcagca tgttacgaat ctctatgccc ttttcaacac gcttcgtgat ggctgcctta
    76081 tcaaaattgg cattggtaat ggtggagaat aagctatcga agacatactt gttaaccata
    76141 gggctttcgt aacccttttg ctcgcggagc gctgtgctgt aaacgcaaat ccccttggtt
    76201 acaaagagca ggagatccat catgttagaa agatcgtcgt ttttaccgca aactcccttg
    76261 attgtgcagc ctactccttt ggctgcttcc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    76321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    76381 nnnnnnnnnn nntttgactc taatatttca ccttgaattc caacggtaat caccttaata
    76441 ggaaccttgc gttttgcctg ttccatggcc atacgggcta gctgaacaag cccaccacag
    76501 cagggaacct ccattttcat cacggtgata gtgtcaacct gtgcatcgtc aattagcgca
    76561 gtaatcttct caacatacga ttccttgttt gagtcgagct tagggcaggc aatagccaag
    76621 gttttaccct tcaggtgttg gctatggaaa ttgccaaggc tgaacgccac acagtcggct
    76681 gcaagtaaca ggtttgagcc gcggaaatga cccgagtgtg ggttaatcag gtgtagctgt
    76741 acgggccagt gggttagctc cgatttcccc gaaaccattc cctcagcacc tgcaatggct
    76801 tgtgcaaagc tgcgagttgc cgaacccgga caacccccac catggtgatg catgggttga
    76861 ggctttacct tgggctcgct tgtcccacaa ccgcaggcat ccttggtgtg gttatgcact
    76921 gcttgcagca cctcatctac gctaaacgtg agtttgtcgc ggttggctaa caaccactca
    76981 acaccctggc gcaagtattc cttttcgtta taatccttga ggtgctttag gtgagcaatg
    77041 atggtattct taccgtttgc caccatctta ctaatggtgt gtacctcgtc gtaaggctca
    77101 gcctcgcggt gctcgatggt aatggcaccc tcggggcatt cgcctatgca ggctccaagt
    77161 ccatcgcaca tcagctcgct gatcagaaca gctttgccat caataagctg tagggctccc
    77221 tcgtggcaac cactaacaca tagtccgcaa ccgttgcact tgtccctgtc aattgtaata
    77281 atgtcgcgct tcatatctat tcgtttttaa ttgttatcaa attgggttga tttgtttttc
    77341 tgagacaaaa gtaggccaac cccactccgc gaattgtaac atatgttaca cacgctgcgg
    77401 attttggagt ttttagggtt gatatatatc aatgccggaa gctgtgcggc gtatcaggct
    77461 tctggaaacc tagtggctat tagtgtttga agatagttta tagttgacgg ttgttagttg
    77521 atagtggaga tagcggtttg aggactccct gtaagtgaga atcttcgttg taggcgatgg
    77581 ggtgttgtaa aaaaactttt ggtattggga tgtttccccg tagggggaaa acaaatccct
    77641 caaatactct caaataaaat taaggaatct tagttccggt caaaaattcc cggatattta
    77701 tggtttcaat tcctttgtat gtgtcgaggg cgtattcatc ggccgtaact acaatttttc
    77761 tgaaattatc gcccgtttca agcagattgc catattcgcg ggttctcaca ttttcatcgg
    77821 ccaacgagag cgttacctgg atgtagattc ggttgctgtt tttatcgcaa atgaaatcaa
    77881 tctctttatc gcctaactta ccaatggtaa cactgtagcc gtgtataagc atgtgcagca
    77941 gaacaatgtt ttccagcaat tggttgatat cgcccggttt atacgatatt atggcgtgtc
    78001 gcagacctgt gtcgtggaag tagtatttct cgccaatctc cagtattcgt ttccctttta
    78061 aatcgttgcg ggagattttg gcaatcagat acgcattttg caggtagttc agatagtcga
    78121 gaacaatatt gttcgacgat ttaatatttt gcgatttcag gtaatcggtt atctttttag
    78181 ccgaaaaaac attaccaata ttgtcggcaa taaaaatgac caaccgctcc agaaaggccg
    78241 cgtttcggat gttgtacctg gtaattatgt ccttgtagag tatggtagta taaatatttc
    78301 gcagatattc aaaaaccaca ttctcgttca acgttaggtt cacaaggtag ggaagtccgc
    78361 caaatcgtag gtatttgttg aaattttcag gagtgttttc caaccggtta aacacgagaa
    78421 attccggata ggaaaggctg tacatggtaa tttcaacata tcgcccggtc agataggtgg
    78481 ataactcgcc cgaaagcagc ttcgcgttac tgccggtaca gtaaatgtcc cacattcctg
    78541 aggtatggaa atgccgcagt gccttttcaa agtcttcaat atcctgaatt tcgtcaataa
    78601 aaatggcggc ttttttcccc tttgttacat tactttcagc aaacgaaaca aggtcagcat
    78661 aggtttttat ggagtcaaat gccaaatctt ccttgtttat gtaaaggatt tggatatccg
    78721 gatttagctg ttgtaccaga tccataacct gaaacagaaa gtagcttttc cccgtacgtc
    78781 gctgtcctac aatcacttta atcaaatcct tgccgataaa aggacgtatc tttgcttcgt
    78841 acatttctcg ccggatatac tttttcataa taaaagtttt gattatgata aaaataattt
    78901 caaatctacg gaaatatttt agtatgaaca aaatatctct gaatttgttt tttttatgtt
    78961 ccaaatggaa ggttcttcac atccttttga tttctggtat aatatgagga gttgatagtt
    79021 gatggttgat agttgatggt tgatagttga tggttgatag ttgatagttg atagttgacg
    79081 attgatagtt gacgattgat agttgatagc tgttagttta tagtggagat agcggtttga
    79141 gcaccctcag taagggaaca tctttgtcga aggcgattga ggaatgtaaa agatcatgat
    79201 aataaaacag tatcccccgt agggaattaa cgatgaatca ataataagaa tatcaataca
    79261 ttactacagt gttacatggt gtggcacagg gtctcatagt gtaagattat aaatctcttt
    79321 gcatttacta tcaaccatca accgtcaact atcaactcaa ttagcatcac agagttacac
    79381 agggtttaac acagagttta acacagtgtt acacagtgaa aatccattta atctgcgtca
    79441 tctgtgtccc attaccaaac accaaacacc aaacaccaag cactaaacac taaacactaa
    79501 acaccaaaca ccaatcacta atcaaccatc aactaacctt atccctttac ttcccttgct
    79561 agtacttacc ccttaaagaa cagctatttc agctcttctt ccaagatttt ttcgagcgtt
    79621 tccacgctta ttcgcttggc caaaatcttt ttgtttttgt ccaaaacgta aattacaggc
    79681 gtggcataga tatcgtacag gttgcggaag tttgagctga acgtaggatc ccaaacattt
    79741 atccaggtta attcgttggc ctttatgtat tccatccatt tgggctggtc gccttgggtg
    79801 taaacggcaa acacctcaaa ccctttaccc ttatatttct cgtaaacctt tttcagctca
    79861 ggggtaacct ttttacagtg gctacaatcg ggatcccaga agtatgcaac agtaaccttg
    79921 gcattcacat cgcttaagcg gtagtatttc ccattggggt ttggtaacca aaggtcaggg
    79981 gctatattgc caataatatt gggctttatg gcttttacac gttcggcaat cttatcgagc
    80041 agtttctggt cggcccacca ggcttggcct cccaggtagt acttttcagc cactgccaca
    80101 aatacaccat ccattcccat tcgttccgag gtttgaaact tattggttag ggtagataga
    80161 atatactgga acatctcctt attggccttt gccttctcta tcagttcaat tgctgctggg
    80221 ataatgctat cgggcaaggg gagtagaacc ctatcgaaat acacattaat tttactctcc
    80281 agtatggggg ttcgcagtag gcgagcatcg gaaaaatcga tattatcaaa gaaatggtac
    80341 cggttatagt tgtagttatg cgcccaaacc agcgaatcgt acttaggagt atccttggca
    80401 atattgtgct tgggtgcttc aacattccgc atggctttta taattgctgc aagcagggtg
    80461 cctttgtagt tggcttccac ctcgtcccag taagctaaaa ccttgttgtt aagctcgttc
    80521 attttatcgc ggatggcatt ggccttaagc gaatcggaaa gcgattcctt cagctgttcc
    80581 gagagttcct tgctctgctt ctgcatgtca atcatgaaac gctggtactt aatgaaccct
    80641 tcgttttcac tagagttggt gaacttgagg ttttcgagta agttatcctt cgatgtggca
    80701 actgaaaatt tctggttctt atcaacaagc acctcaaagt agtttttctc tggcagtata
    80761 acaaggtata taccaccagg caaaagggtg tcgccttcaa acacggcaac tcctttgcta
    80821 tttacagtgg cggtatcttt cacatacttc ttatcgccaa agtggtagcc tagcaataaa
    80881 tcgccctggg ttaatccatc aattttaact tcaatcctgt atccgttttt ttgtgcaaat
    80941 gcacttgcaa aaaccattaa gctcaatgtg agtgtaatca ggaaaaactt tttcatagtt
    81001 cattcaattt tcatggaagc aacaagcaaa gatagcattt ttgaaatgag ccaatattga
    81061 tagccctata tttcaacaaa aatacaacct gttttgttag tgtatcaaat tagataccaa
    81121 atttgatgga tacctggaaa gatgcccggc caaaccgatt agttcgtgag gttagtccat
    81181 accttttaca gcatgcatat aaccctgtgg actggtttcc gtggggcgag gaggcttttg
    81241 cgaaagctaa agcagaaaat aaacttttgc tcataagcat tggttacagc agctgtcatt
    81301 ggtgccatgt aatggagcac gaatcgttct cggacgaaaa ggtggctaaa accatgaacg
    81361 agcgatacgt ttgcgttaag gttgaccgtg aggaacgccc cgatgtcgat cagatttaca
    81421 tgaatgccgt gcagattatt acccgtcgtg gtggttggcc cttaaactgt tttgccttac
    81481 ccgatggccg accggtttac ggaggtacct atttcccaaa ggagaactgg cttagcattc
    81541 ttgagagtct cgatgaaaca tggaaaaacg agcctcagcg catttacgag gttgccgatg
    81601 agctggccga ggggattgca aacactgaga ttatcagggt taaggctgat gtcagtaatg
    81661 ttaaccttaa tgtcgtttta gctgaaaacc tcaattcctt ccgcaatcag ctcgacttta
    81721 agttgggtgg cacgctgggt gcacccaaat ttcccatgcc tggtttgctg aaatacctgc
    81781 tgataaccgg gcaacactcc cagcgaaagg acttacttgc ttttgtcgat accacccttg
    81841 agcgaatggc caacggcggc atatacgacc acgttggcgg tggatttttc cgatactcgg
    81901 ttgatgaaaa gtggtttgtt ccccactttg aaaagatgct ctacgataat gcccagctaa
    81961 ttgaggttta ctccctggcc tataggctca acccaaatcc tttgtatagg agagtggttg
    82021 aggagactat tacctttgtt gagcgtcaac ttctatcgtc aactggtggt tttttcagcg
    82081 cactcgatgc cgatacggct ggtcttgagg gtggttatta cacctggggg aaaagcgagg
    82141 ttgagcagat attgggatat gactcagagc ttttctgtgt ggcttacggt attactccaa
    82201 ccggtaactg ggaaagcatc aatattttat tccgctccct taccaatgcc cagctctcga
    82261 gcctgtttgg attacccgag ggagagattg attacaggct taaaactagc cttataaaac
    82321 tcaagaacca tagggatttc cgtgtccctc cccttgtcga tgataaaatt atcacctcgt
    82381 ggaatagctt gatggtttcg gctctttcca ctgcatatct tacctttggt tacgataaat
    82441 accttcaact tgccttgtca tccgcaaatt atctgataga taaccatgtt aatgatgact
    82501 ggacacttag ccggattaca tgcaaaggta gggtttatgg tactgcattg ctcgacgatt
    82561 acgcctatac tatcaatgcg ttgataaatc tttaccaaat caccaataat actttatgga
    82621 accgttatgc tattaacctg gctcagaaaa ccaaggatat ttttttcgac accaattcag
    82681 gaatgtttta ttacactcca gccagtacag aacttattac ccgtaaaatg gagctgatgg
    82741 acggtgttat gccttcggca tcggctgtaa tggtaagtaa cctgcttgca cttgccgagc
    82801 acgatactag cagtgagtat ggcacaattg cattgcaaat gattgctaac gtgggaaacc
    82861 atattaaaca gggcaatgtt tttgttcata catgggcatt gcagcttttg agtaccattt
    82921 tgccagttgc tgaaatcaag gtaaatggcg atgatttgaa tgctgccgaa atgttgatag
    82981 cctcaaaatt ggtttaccct aagataaagt tcctgcaatc aaccgatata cctaacaagt
    83041 acctgctctg cataggtaat acctgccagt cgcctaccga taattccgaa aatattgtaa
    83101 agtggactaa cgctattagc tttgagtact atgtttagtt ggaacgcata aagcgccttg
    83161 gtcgctttat attaaaatca aatctcttgt ttcaattgta ctgttatccg gataaagtca
    83221 cactaccgcc cgcctaatga atttatgaat tagcaaaatc tctatttaat ttatcctctt
    83281 gtagaaatag aaaatgctag ggttgatttc tcaaaccaca ctttatcgcc tttgaccaag
    83341 cgaaatgata gaatacgccg aaatatctaa aaatgataga ttaatagaga tccttcgctt
    83401 cccgctaggg cgggacaggc tgctcaggat gacaagcgga gttgacagtt gatggttgat
    83461 ggttgatggt tgatagttga tggcaaaaac actaaacact aaacactaaa caccaaacac
    83521 aaaaaactaa acacctctgc acactgcaca ctaattttct ctctaccttt ctaaactccc
    83581 tttttatttt caaaagattt atcctaattt tattgaaaat ttcattgaat ggcgtactcg
    83641 tttgattcat atagggttga agttcttcag aggccttttg ttcgttccga tggctttacc
    83701 actaagcaaa ccgcaaaaat tgaactgttc aatagtaagg gtgttttaat aacaacatta
    83761 agcttgggca ttcctgaaat ggctgatgtt tatgagcgta tcgggaaaat ggagcccgtt
    83821 aacctcgatt actgttacat tcagggattc tccacaattg cctgcaagcg atacctccaa
    83881 attgacaaag cacaaccttt gcctgttgct cagtttactg cctgcaacag ttttttcgat
    83941 tcgccagtaa gcatcgattt gtcattatcc attagcgaaa aagagttcgt tattgatgaa
    84001 tcgtacgtgg ttacagagct ttttgatttt cacctgtcga atctgttagg ctctttccgt
    84061 ttaaccaatt gttatataaa agcggccaag agcaatttta cccaggtcag gtttgaaggg
    84121 gagcagatat cgtttaaaaa tacaatattc gatgtgggtg ttaaggattt tcaggacacc
    84181 tcatttggta gtggcgaggc cattttcact aatgttgatt ttggcgatgg cgatgtttcg
    84241 ttcattaatg ctgggttcaa taattccgat gtcgatttta gggtgtgccg gttcggcacg
    84301 ggtaaggtcg attttcacta tgcccgattt ggcggaggtg gtgtatcgtt tgaacgtgtt
    84361 gactttggta gtgggcgagt cgattttcgt gctgccgaat tccgtgctgg tcgaacctcg
    84421 tttaaccgtg cctccttcca tgagggggag ctgaactttg agggtgccga aacgccaaag
    84481 ggtaaactca ctttcaaacg cgcagcattt ggaaacgtta cccttatatt cgacttatac
    84541 cagggagcaa aatccgaaat cgtttttgag cgctcgttat ttagccaaaa cattagcttt
    84601 tccgaggcac agcttggtaa gctaattttc tccgattgtc agttcaattc tatggttaac
    84661 ctgcatgtaa agcagagcga cgaaattaac ttagcaggct gcaccattcg cgatatagtt
    84721 gaattttata cccatggcga tccacccaga atcaatagct taaacctgac gggtgtacga
    84781 ttgctgggtc aaatctatat cgattgggac gccaatggcg ttcgtagctt gatttactct
    84841 caggggtgta ctacagttgc cgagaaggct gaacagttcc gaattctcaa ggaaaacttt
    84901 aatgcccttg gaaagtacga cgaggaggat gctgcctatg ttgaatttaa acgatgccat
    84961 atgagagcat tgctcttgag ctcaaataag cagggcaagc tgaggcattt aatcaatagg
    85021 attatttact gggcaaagaa actgcttttc gattacatgg gtctttttgc cacttcgccc
    85081 cagcgagtaa ttttcagcat tttggtggtt tacgtaatat acagcctggc atatatcgct
    85141 ataatgggtt tgggtttggg gcatattatt gcaacatcgg ccagtggaga tcagttgggg
    85201 atagttgccc ggggttttta ctttagcgtg gttacctttt tcaccattgg ctatggcgat
    85261 tttgcaccat caggaatagc aaggtttgtt gccggcaccg agggttttat gggggtattt
    85321 ctgatggctt actttactgt ggcctttgtg aggaaaatac tccgctaagg gtgtagtgta
    85381 tagtgtgcag tgttcagttt tcagtggtgg gtgatagttg atggttgatg gttgatagtt
    85441 gatagttgat
//
LOCUS       DAOOWJ010000002        59889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_554_length_59889_cov_7.958418, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000002 DAOOWJ010000000
VERSION     DAOOWJ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 59889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59889
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_59889_cov_7.958418"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLW22_00365"
     CDS             complement(<1..388)
                     /locus_tag="PLW22_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73181.1"
                     /translation="MKQTLLSFLSIFIMNFASGETIKVKIFYDKEWKICKESTASYYR
                     ICNWNTDKNYFDGEFHDYLINGKKIVEGNYVNNIKSGLFIFYYESGIEWIKGEFKNDI
                     PIGIWTWKYPNGNTHFTLKFENNNFYF"
     gene            complement(401..1090)
                     /locus_tag="PLW22_00370"
     CDS             complement(401..1090)
                     /locus_tag="PLW22_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73182.1"
                     /translation="MKKILYIAIFLILITEKSFSQDYIVQVNDRNRAIELISQASTFI
                     ENNELEKAYITILESITIDSTFRPAYLYLFKIFTLDNLFADKVLPFLIKGRNIYNDDD
                     ELTFYIAEIYRIKSEYEKAKIEYSKAINLSKNNGENFPLIYLYYLNRGNCNLKLNQID
                     SAILDYTNSIKLKPNHSNTYYNRGISYYAKGTIDKACEDWSKSLKLGNKNANVYIDKF
                     CKSTKNNKSLK"
     gene            complement(1160..3148)
                     /locus_tag="PLW22_00375"
     CDS             complement(1160..3148)
                     /locus_tag="PLW22_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU73183.1"
                     /translation="MKRLLLIALGLVYLTSGNISAQPTKKLEKADALYNAGAYFDAIA
                     LYRDDIDKIKDKKELSLYLFKIGNCFRQIGEPRQAEQWYEKAIRRECPNPKVYYYYAE
                     MLKMNEKYDLAIEQYQKYKVLVPNDPLAEVGIQSCELAKQWIATPAGYEITNMRAINS
                     KFSDFSPAYASTDYNILYLTSSRNGTIGNKTSAVTGENFSDIFITELDNKGVWSAPKP
                     LDETINSQFDEGTPSLTPDGNNLFFTRCKVSKRNKLGCQILYAKQSDQGWLTPKELEL
                     AADSMIVAHPAISADGTELYFVSNIPGGNGGLDIWKITREAGSEEWSAPINLGPQINT
                     PGNEMFPYIHPDGTLYFSSDGHPGMGGLDIFKAKKNDEGQWVIENMRYPVNSTADDFG
                     IIFEKEREAGFFSSRRNGGRGADDIYMFYLPPINFNMIGKVMDEKTRTTITDATVKLV
                     GSDGTIATMQTQSDGSFRFNLVPNTDYVIVASKKGYLNSKAKETTKGLTKSQDFNVSI
                     ALTSTAKPIEIPNIFYDYDKWELRPESKEALDNLIQILNDNPNITIELASHTDSRGSQ
                     EYNYELSQKRAQAVVNYLIENGIATDRLKAKGYAQTQPKVVDDNLAAQYPFLTPGTVL
                     DKKFIDSLTSEDQKETVHQINRRTEFRVLREDYQSSGK"
     gene            complement(3452..5647)
                     /locus_tag="PLW22_00380"
     CDS             complement(3452..5647)
                     /locus_tag="PLW22_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOU73184.1"
                     /translation="MPKLRFRAFGEATNRIPVDMNGEGKAESLVDIFAINVFDRPKMK
                     KYLSEEAYESIITSIDEGKKIDRKIAGQVASAMKAWAIERGATHYTHWFHPLNGSTAE
                     KHDAFLSIDIKGQPIEVFKGDMLVQQEPDASSFPSGGIRNTFEARGYTAWDPTSPAFI
                     LENTLCIPTVFVSYTGEALDFKTPLLKSLALLDKAALSVLEYFDKDARKVYATLGWEQ
                     EYFLIDESLYNARPDLALCGRTLMGHAAAKDQQLEDHYFGSIPARVSAFMRHFEVEAL
                     KLGIPIKTRHNEVAPNQFECAPIFEEANLAVDHNQLIMSLMDRIARQHGFRVLFHEKP
                     FKGINGSGKHNNWSMMTDTGTNLLSPGKTPKNNLQFLTFLINTIKAVHDHSALLLGSI
                     ASEPNSHRLGAHEAPPAIMSVFIGKTLSAVLDELEQKVTDQKMTPDEKTELKLEIIGK
                     IPEILLDNTDRNRTSPFAFTGNRFEFRAVGSSGSCASPMIVLNTAVANQLMKFKDEVD
                     KVIAKGIKKDEAIIQCLRKVIIESKKIRFEGNGYSQEWVDEAARRGLPNISNPLEAFK
                     TFLLDSSVKLLTGNGILTKRELEARYEIRNEIFVKKVQIEARVIGDLAINHIIPTAIA
                     YQNTLISNVKGLKELFPNEWEKLADHQHALILEISEHIHAISHQVNEMIERRKEANQV
                     EDIVEKAHLYGKNVKPYIESIRTHIDKLEMIVDDEMWPLPKYREMLFIR"
     gene            5995..6477
                     /locus_tag="PLW22_00385"
     CDS             5995..6477
                     /locus_tag="PLW22_00385"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOU73185.1"
                     /translation="MESDNRKYSNDEITVFWRPGECVHASICYTKLLSVFNPRKRPWV
                     NMNGASTERIIEIVNECPTNALTFMWNDPEKNKQERSHKCVREFTPEEVEAFEVTPVK
                     IQVMPNGPLLVSGDFQIIDADGKEVKSMKMVSICRCGHSNSQPFCDGTHFKKGFRDSE
                     "
     gene            6470..6688
                     /locus_tag="PLW22_00390"
     CDS             6470..6688
                     /locus_tag="PLW22_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOU73186.1"
                     /translation="MSSKMNQPKKIAAKLHLSKGGPIRVEGRFFIVNLDGESLIPDNS
                     KEVFLCTCGLSRNKPFCDGSHKGGKSNG"
     gene            6883..7224
                     /locus_tag="PLW22_00395"
     CDS             6883..7224
                     /locus_tag="PLW22_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOU73187.1"
                     /translation="MIFGIVSFGLGVLGIFLPLLPTTPFMLLSAFLFIRSSGRLYRWL
                     INHRLFGKYIHDYIERRSIPRRVKWYTLGLLWASILTSVATLDVGNVIRLILLLIAVG
                     VTAHVVKLRNS"
     gene            complement(7231..8223)
                     /locus_tag="PLW22_00400"
     CDS             complement(7231..8223)
                     /locus_tag="PLW22_00400"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOU73188.1"
                     /translation="MNTKLNIAIMAGGNSSEANISMRSATQIAGWLDPEQFNAYKILV
                     KGTTWTLEHPQKGQIPVNKNDFTVDIDGQKLSFHCALIAIHGTPGENGLLQAYFEMLQ
                     IPYTTGGVMNSSVTFNKYYCKELVRETGVNLAKGLLLRRGQKVDAQAILDTLGLPIFV
                     KPNESGSSYGVSKVKQASDLMPAIEKAFAEDKTIILEEFIPGREFTCGVFKYQNGEMI
                     MPVTEIVPETEFFDYEAKYRNKSKEITPAPIPESLTSAIQQFTSKIYDRLQCHGVVRV
                     DYIVNNDKIYFLEINTVPGMSEASIIPQQVKAMGGNMRQFFTLVIYDALNRSRE"
     gene            complement(8320..9303)
                     /locus_tag="PLW22_00405"
     CDS             complement(8320..9303)
                     /locus_tag="PLW22_00405"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOU73189.1"
                     /translation="MADLSTTYMGFKLKNPYIASSSGLTDSVEKAALLQKHGFAAIVV
                     KSLFEEQIMMDVDAQRMNNMFNSYSEAEDYIAYYTRKHALSNYVELIDGMKQKLSIPV
                     IASINCSSADSWIDFAGDIQAAGADAIELNMFIMPSDVKMKGDEIEKIYIDVARKVAQ
                     TLSVPIAMKIHHYFSGMANFAVELSRTGIKALVLFNRFYQPDVDLNTLKVTSGSILSH
                     PDENAEVRRWLSILSGRVACNLAASTGIHDWQTAVKNILLGANAVQVASAMYKSGPEI
                     LEPWIEETSKWLDANDFESINSITGKLRQADSIKPLAYERAQFMRYFSDAR"
     gene            complement(9335..11134)
                     /locus_tag="PLW22_00410"
     CDS             complement(9335..11134)
                     /locus_tag="PLW22_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU73190.1"
                     /translation="MARIKDIISPFTAWKNVVKEPVTIKEPLKREAAPRYRGFHKNDV
                     DTCIGCGTCESICQNAAIDMVPVEGIETKNGDSGLRPRIDYGRCCWCALCVDVCTTGS
                     LSMSNHFKWVDSDPDVFRFIPGVDIKEWDDEPNGYKKPGPNYELYSKERVHMPELKPE
                     ERDKSFIEIVRGYSREQAIKEADRCVECGICVATCPAHMGIPEYIKAIRNDDLNEGLR
                     ILYDTNPLPEVCGRICTHKCETVCSVGHQGDPLSIRWLKRYIADQQPAENYKQILETE
                     NISKNGKRIAIIGAGPAGLSAAHYLALMGYECTIFEKLPLPGGMMRYGIPEYRLPYDA
                     LDKDIDYIKSLGVEIRCNTTVGKDITLEELHNQFDAVFTGTGLHLGRSTRIPGTDHPH
                     VYQAVDLLRDVTMGKEIPAAPSIVVIGGGNVAMDITRTLARIQNQRYGKVNIITTSLE
                     SENEMPADREEVVEAREEGATIIPGWGPVKIEIENNKIKGLHVVKCVSVFDENRRFNP
                     KFDDTQTDFFAGEMVVESIGQGMDLSYLPESIKDNLKLDSRGRIVVNDYFQSSVEWLF
                     VGGDIIQGPDVIHGIANGHKAAIGIDRFLGGKK"
     gene            complement(11157..12350)
                     /locus_tag="PLW22_00415"
     CDS             complement(11157..12350)
                     /locus_tag="PLW22_00415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOU73191.1"
                     /translation="MKFSKLFKINEDRSLYPPLNSEGKVDIDLRSHKYVKLWHGPQHP
                     GITGNMSLELTLLGDEIMDCETHVGYLHRGFEKLMERRKWIQCFPIVCRITVPEPDFN
                     EYCFAAGVEELAGIEIPETAQWLRTLTLEMARLQSYLMWIGGQAGSLGMGIIGQWSNY
                     CRDLVLDRFEELTGGRIYHMYIIPGGVRGLLPDGFEARMEETLKTIEEFMVDIDRVMF
                     NNAVFKKRTVGMGYIDPKWIYQFGITGPNARAAGIPDDVRKKYPYLKYPELDFEPVVG
                     KDSDIYTRADVRRRDLLLSVDLIRQILAKMPKDGDFKASTPNVLHWQIPAGETFIRSE
                     CTRGEFAYYIVSDGSEYPRRINVRGPSYTHAVALLEKMIINTNLADVAGLMTSLHTYP
                     PEIER"
     gene            complement(12340..12870)
                     /locus_tag="PLW22_00420"
     CDS             complement(12340..12870)
                     /locus_tag="PLW22_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOU73192.1"
                     /translation="MNTLLDNIKTVFPNAEVWTQRKGLYHMSIPKEQLVTLVTHLKEV
                     EGYLHLVQLTAVDWIEKNQFQLTYILNNPNKRIDIMVKTFIPRDNPTMETIHHLWEQA
                     ATYQRELREMFGIDFPGSPRVDKPFILEGWDNIPPYRRDFDTKKYAEETYFPRPGRET
                     HDPKEYMKKKLYPDEI"
     gene            complement(12882..13523)
                     /locus_tag="PLW22_00425"
     CDS             complement(12882..13523)
                     /locus_tag="PLW22_00425"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOU73193.1"
                     /translation="MSKSDLEKRTVMSPDGEPIEINPLQDYFCDARPKVHEPVLPIFE
                     KFLNWARSESLWVLGFGTGCGAIEMRPLMTPRFDAYRFGIQWRPTPRQSSVFIISGYL
                     SVKTLKRVIRSYEQMQNPKFVIALGSCTINGGMYWDSYNTIKRLDHYLPVDVYIAGCM
                     PRPEAILAGFEKLKELIRQGKGEGANLYAKNFDWYKANQKKIIKDWDIPDYNW"
     gene            complement(13552..14457)
                     /locus_tag="PLW22_00430"
     CDS             complement(13552..14457)
                     /locus_tag="PLW22_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOU73194.1"
                     /translation="MEFGIKQILYLLLTLFIVLNYGLILQGIMPKIAARVGRRYGIRW
                     YQPYLDLIKNHAKRSKITHGVMYYLGPVFRLTGGIGLLFFLPVIYGSPMFDNLSFAGD
                     LTLVLFFQFFGVLGMALGAGEGGHPYSAIGVSRGLSQVTALELPLTLGVISIAMQYHT
                     LNISDIVAAQQGGILNWTIFTNPFAGAALMLTFLGSMGRAPFDVVLAPQEIPIGPPTE
                     YHSGFLGVLQLNRAIFPVAKLILFMNLLYGGATSWPELFIKTFFIYLWSIFVGVAFPR
                     FRVDQSVRWFLGVPLLFGIIAILLI"
     gene            complement(14458..17583)
                     /locus_tag="PLW22_00435"
     CDS             complement(14458..17583)
                     /locus_tag="PLW22_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU73195.1"
                     /translation="MVNPIHIVSIALGVAFALGFLGKREKLALGVVYTALAAMIAISA
                     GWLSHFWGNATESVQIFTGGFKPPFAINLQMGLAESIFTLLINTVGLLGAIYLAKELL
                     QAGKNMMIVFLVLVLGLNVMVMTRDLFNLFVFMEIQSIAIAGLVILNPDRKSIPAGFK
                     YVIATGLISGLLLLGTVFIYYFGGTLNIDFISQASLLYVKGGYVAIFLVLLSIVLELK
                     PFPANGWALDVYEGAHPGIGALISAASATAGIFVLDKLMVFGNFTLFNALSIIGAITF
                     MASNLLGIKQENTRRLLGYSSVGQIGLIMTIIGLKPYLGENTKFIALAILLSHYLAKA
                     GLFWLAGIVNVDKLKDWGAIRGHRIYLFLMGTFIFTLVGFPPFPSFFGKWQLVMQLAS
                     NHHFWLLAIILLGSLFEGVYLFRWLGYAIKWDEVEQNYPKIKLHQFIPTALFAGLTYA
                     LGFYFGEITGYKSTFIYMATAFIAFLYIVDFLPVYIKNTLAIGAMVLYYFSHQDTFHG
                     LTSIFFIIFVIGGIITLLAGYAFKGKRTGFYPLVMMMFAGLIGLLEAKTTLEFFFAWE
                     LMTAGSYFLIIRGKKSMEHALSYILFSVGGAYLILAGFSIAQLGHTGMGLEILKDIHY
                     YAPIAFTLMAIGFMTKLASIGLHIWLPGAHAEAEADVSPMVSAILLKAGVLGLVMLFL
                     SMGPQKLFGIDVLYALSWVGVLTALMGNMMAAFQEDAKRLLAYSSIGVLGYALFGLTM
                     MSHLGWLASLSIVVTHFMFKSLLFLAIGGVVMRTKTKYMYQMGGLIKQMPFSFVSVLI
                     GIIVIAGLPPLTGFGGKWLMFNAIMQNGWYFQAGIAFFAGVISFLYCFRLIHTIFLGQ
                     PKDELRNVKEAPFWMLVPQYIIITVLLVLSALPNSMLKPIGQILLNYFPEGALVWHGQ
                     LAIGPFGYWNGKLILAIVVLIFGAVFAWLYIINRKAQKVKQFNIVYSAERPFRPETTH
                     FAYNFYAPYKKAVGFLVAPGITNFWDAVGEGAHAIADKIRSIYNGNGQTYLVHILGFT
                     VIVYILINGGF"
     gene            complement(17577..17951)
                     /locus_tag="PLW22_00440"
     CDS             complement(17577..17951)
                     /locus_tag="PLW22_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HOU73196.1"
                     /translation="MAQYLTLEFLAMLIGFMLIVVGLWGMITQKNLIKIVIGFSLFDT
                     GLNIVIVSMAYIKNSTAPIIDKAVDVSQAASKVADPVPQALVLTSIVIGLGVTALMLA
                     YVLRMYREKKSLDINSYTDLKW"
     gene            complement(17954..18655)
                     /locus_tag="PLW22_00445"
     CDS             complement(17954..18655)
                     /locus_tag="PLW22_00445"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HOU73197.1"
                     /translation="MIKKGILLLLLVGFVAIIGSLLINFTGSNELNPLARYYAENGPA
                     EVGAANLVTAVVVTYRGFDTLGEVTILFIAAAIVGFFLKVMNADNGRPNKLRKTSEIL
                     ETASTLLVPTIFMLGVYIFVNGHLTPGGGFQGGAVIATGVMMIILAQPTSRFNHKLIS
                     VLESVSGVGFVAMGILGLILASGFLNNAFLPLGKLGSLLSAGAIPIIYVFIGIKVGSE
                     LTGILDSLKENQNEI"
     gene            complement(18648..18890)
                     /locus_tag="PLW22_00450"
     CDS             complement(18648..18890)
                     /locus_tag="PLW22_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOU73198.1"
                     /translation="MSLILAIFLGVVILVLAVTAIYHKNTKVAIIATGAVGLFASVLY
                     LLLAAPDVALTEASIGSGLSTIIFFYVLNKIRHSND"
     gene            complement(18893..19234)
                     /gene="mnhG"
                     /locus_tag="PLW22_00455"
     CDS             complement(18893..19234)
                     /gene="mnhG"
                     /locus_tag="PLW22_00455"
                     /inference="COORDINATES: protein motif:HMM:NF009314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOU73199.1"
                     /translation="MEIRELIGALVTLVGSIFIFLGGLGIVRMPDLFNRIQAGTKAST
                     LGTILSLIGLAIVNPAWIWKLLVIMFFVLITNPVSSNVISRAAHFRGTKMTDKTVADL
                     LVESENKTEEE"
     gene            complement(19234..19473)
                     /locus_tag="PLW22_00460"
     CDS             complement(19234..19473)
                     /locus_tag="PLW22_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOU73200.1"
                     /translation="MAGVLMALGILLALLRFIKGPDVTDRTIAFDVMTVASISVIALI
                     SHFAGRIIYLDVALVYGLLSFLSVVVIGRYIERGL"
     gene            complement(19487..19981)
                     /locus_tag="PLW22_00465"
     CDS             complement(19487..19981)
                     /locus_tag="PLW22_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOU73201.1"
                     /translation="MNIRNVANLFVLLFLIWLLLTGTFELTSLLLGLALAVTLALIFG
                     KNSNVFGRFRMTPKVFVYSLIYLFVLSWEIVKSNIDVALRVLNPKLPINPGIVKVKTR
                     LKSPIGRMILANSITLTPGTLTIDMKDDELYIHWIDVKTLDTEEASRKILGKFEKLLE
                     VIYG"
     gene            20181..20870
                     /locus_tag="PLW22_00470"
     CDS             20181..20870
                     /locus_tag="PLW22_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU73202.1"
                     /translation="MVESKKSCLLCNNKSECFRQLSDSQLDFANSNRAEISYRKGEMI
                     AKQGNFYTHILYLQSGMVKVYKELPDGSNLILSIFGVGSLIGLPFLFKNQILDYSVAA
                     LDDTTICAIDRHAFEKLIHENGEFAAEVVNQLNQCTLYNYDRLVSLTHKQINGRFADT
                     LLYLSRVVYKANPFNLTLSRKDLAEYTGVSVMSVIRAIKEFKNDGIIDISGNRVEILN
                     FSQLERISRTG"
     gene            complement(20967..22022)
                     /locus_tag="PLW22_00475"
     CDS             complement(20967..22022)
                     /locus_tag="PLW22_00475"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU73203.1"
                     /translation="MNQNRLLDPDDDLENGTAEQADLYEHFRIEVDKGQTPTRIDKFL
                     TDKIRNVSRNRIQGAADAGNILVNGKAVKSSYKVKPLDTISIVLAYPPRETDIKPENI
                     PIDIVYEDEALLVVNKPAGMVVHPAHGNFTGTLVNALLYHLRDVPLFKSGEVRPGLVH
                     RIDRNTSGLLVIAKTEMAMNHLARQFFERSTDRLYIALVWGDMETDSGTITGHIGRSI
                     RDRKKMQVFPDGEQGKHAVTHWKVIERFGFVTLIECKLETGRTHQIRAHMEYIRHPLF
                     SDEVYGGDKILKGNLTTKYKQFVENCFELCPRHALHAKTLGFTHPLTNKKMHFDSQLP
                     YDMSALIEKWRAYGKGI"
     gene            complement(22019..23932)
                     /locus_tag="PLW22_00480"
     CDS             complement(22019..23932)
                     /locus_tag="PLW22_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU73204.1"
                     /translation="MRKALTILVFIFVSSLCYGQEVLVNLSGYRTISDVTKKSNYTKT
                     NLSLPFFDDFARNTPYPTPELWEPSNAISNKTYAINPPTVGAVTFDAVNRKGVLHQHL
                     TTTPQPADTLTSLPINLNYPATDSIYLSFNVQPGGLGYQPSPNDSLVLELYSPSENRW
                     VRGWASSVDFNANKIAFYNHLLNNKDEKSSTKLDSTFFTVNINIDNPIFLENGFRFRF
                     IGYASLMENATVPGYKTNSDHWHIDLVYLNRLRNWDDTIYNDIAFRKPIKSMLKNYTS
                     VPWTHFADAEASEIKNPREFSIVYNNLGPTTWNVTRRFAVTNLSTGNEYAFSGGAENI
                     YGYEEFTYTRPFDYTFTSAWPDSAKFMLKTFLQTDFDESTKHLRYNDTLVHQLNFYNY
                     YALDDGSAESGYGLFGEGTINAMIAQKFYSYKADNLVGVMIYFNRSYQDANNQPFKIS
                     IWADNNGKPGELLYQRSTTRPLFTDSLNCFTVYRTDPVSIPVGNFYIGWQQSTIEFLN
                     VGFDRNTNSRERIFHTLPGYWTNTQFEGTIMIRPIFGKLYQNPTTAPISTTSNIRVYP
                     NPASEKIYIDVGNGIAVNSYQLISITGQVIESKSVYQNEPIDVEHIPTGMYLLKVNLA
                     NGQSLTSKVIIKQ"
     gene            complement(23940..24818)
                     /locus_tag="PLW22_00485"
     CDS             complement(23940..24818)
                     /locus_tag="PLW22_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOU73205.1"
                     /translation="MTTKTAFEKVKQYLLKIYKIPYVNTIINGLGLILGVLIVLMIFL
                     RIYTRHNQEQPTPTLLGMAVEEAAEVIADNNLRIEIADSVYIFNKKPGSVISQNPEPG
                     TMVKKNRRVFVTINAKNPIKIEIPNIVGYTLRQAKAILEQEGFEVGTLYFRPDLGLNN
                     VLEQRFEGKIAEPGTRIPKGSKIDLVLGQGMQGERTGIPLLIGLKLNDAVSRIIEASL
                     NVGRIRYDETIITHNDSLDARVYSQYPAYMENATLGFGTRVDIWLTLNQARIPKIERA
                     DSVNTMPAKHADEIIE"
     gene            complement(24877..26001)
                     /gene="mqnE"
                     /locus_tag="PLW22_00490"
     CDS             complement(24877..26001)
                     /gene="mqnE"
                     /locus_tag="PLW22_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOU73206.1"
                     /translation="MEPIVKTIAPNINDEFLKHITSKVMDGQRIQPNEAVYLYEKADL
                     ALLSQLANITSSRINGDYVLFNKNFHIEPTNLCVFNCKFCSYHKGAGDPESWVYTLDE
                     IKNIARNYQNSDVTEVHIVGGVHPKWDLDYYGKMIKTVKEILPNIHVKAFSAIELDYV
                     IRKSKLSYAEGLRKLKEYGLDSIPGGGAEIFDPAVRAKICPDKASGEVWLAIHKAAHQ
                     NGIQSNATMLYGHIESYKHRVQHMEAIRNLQDETHGFNCFIPLKFRSANNPMGYLGEV
                     NLTEDLRNFAVSRIFMDNIKHLKAYWPMLGIQNTRLAIHFGADDIDGTIDDTTKIYSM
                     AGAEEQKPVMTVNRLTQLVREAGKIPAERDSLYNIIKVYS"
     gene            complement(26087..26242)
                     /gene="rpmH"
                     /locus_tag="PLW22_00495"
     CDS             complement(26087..26242)
                     /gene="rpmH"
                     /locus_tag="PLW22_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOU73207.1"
                     /translation="MKRTFQPSIRKKRNKHGFRERMSTHNGRRVLAARRAKGRKRLTV
                     SDEIFRK"
     gene            complement(26608..27228)
                     /gene="rsmG"
                     /locus_tag="PLW22_00500"
     CDS             complement(26608..27228)
                     /gene="rsmG"
                     /locus_tag="PLW22_00500"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOU73208.1"
                     /translation="MEQIAQYFPELTQAQRDQFAQLEPLYRLWNSQINVISRKDIDNL
                     MIHHVLHSLAIAKVVQFAKGTTIMDIGTGGGFPGIPLAILFPNCKFTLVDSIAKKTKV
                     TSEVAKAVGLHNVEVITARAENLKQEWDFVVSRAVAPVPKLMAWVEKNIHPGGINNLP
                     NGLICLKGGDIKEELKPYHKWATKWNIDDFFEDDYFVEKYVIHIEV"
     gene            complement(27240..27857)
                     /locus_tag="PLW22_00505"
     CDS             complement(27240..27857)
                     /locus_tag="PLW22_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU73209.1"
                     /translation="MVMIPENLSEKAKYDLVLVDKAIKGDQKAYAELMDRYRDAIYYM
                     LLKMVNNKSDAEDLTLEAFGKAFKNIHQYTPNFAFSTWLFKIASNNCIDFIRKRKANL
                     ISIDQGPEDAEGNSMSPSSVIPASTPDPEENLIKEQNIRLIQEVVSKLKPRYRKLIEL
                     RYFKEYSYEEIAEELDLPLGTVKAQLFRARELLSNILSNLPPHHK"
     gene            complement(27851..29014)
                     /locus_tag="PLW22_00510"
     CDS             complement(27851..29014)
                     /locus_tag="PLW22_00510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU73210.1"
                     /translation="MKITTCLIMEIIYNLSIYQKVIGVVFLVFVLIQLYFYLVFYLRV
                     IRKKFNDLPENDEQYPPLSVVICARNEEENLKHFLPKVLEQDYPDYEVVVVNDCSSDN
                     SDLVLESLQKKYPRLRVTTIKEDEKFTHTKKLALTVGIKAAKNEWLVLTDADCFPKSN
                     KWLRYMASNFTNGTEVVLGYGGYLAERGLLNKLVRLDTLFIALNYFGWALARRPYMGV
                     GRNLAYKKSLFFKNKGFANHVGIDSGDDDLFVMEVATPSNTAVEYRPEAHTLSVPRKT
                     YSTWLRQKRRHLSTAKFYSSNLKTRLGLEPLSRILVYILFALTLIFKILPLISTALLL
                     LVIIVKHTIAAIAAKKFEEKKLMHIAFWWDLFSLYFYAKLLIFNTLNRRQQKW"
     gene            29164..30297
                     /locus_tag="PLW22_00515"
     CDS             29164..30297
                     /locus_tag="PLW22_00515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU73211.1"
                     /translation="MSFSRFNLNPAVLEGIEAMGYTKPTPIQEQAIPVILEGRDLLAC
                     AQTGTGKTAAFMLPILSLLSAEEHDASSVNTLVIVPTRELAMQIDQQVEGFSYFAPVS
                     SIAVYGGNDGSNWDRQRQALEHGAEIIVATPGRLMQHIQMGYVNLKGVQHLVLDEADR
                     MLDMGFYDDIMAIIAYLPKQRQTLMFSATMPPNIRKLAKSILVEPVEINLAVSKPAEN
                     ILQAVYHVGASQKLSLLASLLSKKEVVQSAIVFASTKAEVKSIESKLHKEGLSVAAMH
                     SDLEQKQREEVMRNFRNRKVQVLVATDIVARGIDIEDIDLVVNYDVPSDPEDYVHRIG
                     RTARAQSDGVAITFVSPDQRGKLHGIERFLGTKIFVINYIPNS"
     gene            30382..32949
                     /locus_tag="PLW22_00520"
     CDS             30382..32949
                     /locus_tag="PLW22_00520"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOU73212.1"
                     /translation="MKGRVADDTGRPIPYASIYSKELRQGTSSNSDGDYMLRVPSGEY
                     SFVFRGLGYETVERRIKVDVGEVTLNITMPVKPFLIAPVTVGGKGEDIAYDVVRKAIA
                     MAPYYRNLVSEYEAEVYLKGTFKVDKLSWLVKRSIKDSPNPPKEGSLYLQESYNNVRF
                     TAPEKFDQRVKMIRSNFPGELNSTDNILGFVSANLYQPKVGEIILPLAPYAFNHYLFK
                     YEGYAFQDNRALVKVKVIPKRKSRQLVEGYMYIAEDYWNLHEIDFTVQSLAGPFRIKQ
                     TFGEVERNAWLPISQYFDIDANFLGNVGRIQYVTAIKYLNVKLNSKVKPPVKIDIKEA
                     ETKHIAEDTQVKRNTSPRKQREDARMEQLLAKDNLTNREMYELQKLMQKQVRQSDSLP
                     NKLEVPNPLTITVDSVATKPDTMAWQQVRPVALNAEEFKVNREIDSKLQMSPDSVSAD
                     STGKANVLKTLYMGHTWYNANKKRTFLFSGFAGPNEFRFNTVDGFVVGASGSYRKQYS
                     RNGIYIKQSAYYATARKTLMGETDFRLSYANMNRGLASIRMGWISYDFNGDAGISPLT
                     NTSASLWFGRNYMKLYENRYVSLSNRIDPLNGFEVYTSVSFARRVELQNNTDFIVYQQ
                     NLRWYTPNVPANEDATPQNLSTHSSLEGSIRVRYTPFYHYRIRNGRKQMLYSRWPTIT
                     FQVHKALPNLLNSNSDYLRWEAMVNQIIRTGPSNRISYKFIYGDFASKGSLYFVDFKH
                     FNTMRVSVVIPSFSEGYQNISYYRRSTSECYAQAFLSYQSPYLALKYLPFLSNRIWLE
                     NLQLTFLKTAGYKPYYEIGYFLSQIGVVGGIGVFGSFEGKQFHSLSVKVSLIIPGFE"
     gene            33115..33690
                     /locus_tag="PLW22_00525"
     CDS             33115..33690
                     /locus_tag="PLW22_00525"
                     /inference="COORDINATES: protein motif:HMM:NF027619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU73213.1"
                     /translation="MRKKDEAKPEVIFEASLDVLINHGFVNFSMGRVAKQAKIAVGTI
                     YLYFKHKDDLLNQLYFKLYKESVDRFLEHYNPNNSFRESLKLVWVNYLKHRINHYKES
                     HFLEQYYKSSYINDSHKQMAEQMKKPIHDIIQKGKELGLVRSDLDNEMIFLGMLGFLR
                     ELADEHVGNVYILDEDRINKAFEMSWNMIKA"
     gene            33716..34522
                     /locus_tag="PLW22_00530"
     CDS             33716..34522
                     /locus_tag="PLW22_00530"
                     /inference="COORDINATES: protein motif:HMM:NF039830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5692 family protein"
                     /protein_id="HOU73214.1"
                     /translation="MAIPFWQYLVSMPLYIVLLMLAVEFMRTNYRFAAVFWVLSLFTF
                     PLWFNNLEGWFRWAKTLSVLLPTAFVVGCARIAQYEKRDGWWTIFRKDWVLWFLYAIL
                     GLNILEASLKDFEMGNWFNGLSGLILIVTIPLVKSGKGKRYGWKISEEKPGDLIAYTD
                     AIWNFLYTSWNIAFVYAENPGYAASSLCILLAAELYPVIKRRPELYVQARVYTLAIHI
                     LIRATYDIFTPIMDSSAFASQNVVFWWGIVNFSLHLPYLIWYFFLKKTEK"
     gene            complement(34756..35448)
                     /gene="rsmI"
                     /locus_tag="PLW22_00535"
     CDS             complement(34756..35448)
                     /gene="rsmI"
                     /locus_tag="PLW22_00535"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU73215.1"
                     /translation="MSKLFIVPTPIGNLADITLRALDVLKTVNLILAEDTRTSSNLLK
                     HYGIATPLKSHHKFNEHQTVDYLASRIEAGESVALISDAGTPGISDPGFLLVRTCVER
                     GIEVECLPGATALIPALVQSGFPCDRFIFEGFLPQKKGRNKRLEALKDEERTIILYES
                     PFRIVKLLEQMLPIFGADRQVCIARELTKLHEENFRGSLAQAKEHFSSKSPKGEFVVV
                     VKGKTNNEKEED"
     gene            complement(35461..36180)
                     /locus_tag="PLW22_00540"
     CDS             complement(35461..36180)
                     /locus_tag="PLW22_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73216.1"
                     /translation="MQYLKNARNQLVEAVAMIDKALTAGKLPSIERDILLAKLASIYE
                     DLLLDRQQVASRTHEKPQVVPSGKMVEPHEKTIASIPETKKTTVVEESAEPSSIPTAK
                     QEPNPVKADISPSPKPTPASTQQSHEESSAILADKYQGKRKFRNEVIAEQHQKVDMQS
                     KLQNKPITDLAKAIGINDKFLFIKELFGGDSDLYNQTIKHLNQLTDLNEAIIYLQENF
                     SWDPTNEVTTLFVDLVRRKLS"
     gene            36267..36854
                     /locus_tag="PLW22_00545"
     CDS             36267..36854
                     /locus_tag="PLW22_00545"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU73217.1"
                     /translation="MPFLENPVSGKRKGYIEVIAGSMFSGKTEELIRRLKRAKYAKQR
                     VEIFKPMIDTRYSEQEVVSHDANSILSTPVSSSGNILILAQNVDVVGIDEAQFFDMNL
                     PNVCSQLADMGIRVIVAGLDMDFRGKPFGPMPNLLAIAEYVTKVHAICVKCGDLAQYS
                     HRKTDADKLVLLGETDIYEPLCRLCYNEVTRKNRK"
     gene            36887..37357
                     /locus_tag="PLW22_00550"
     CDS             36887..37357
                     /locus_tag="PLW22_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73218.1"
                     /translation="MQKINRLGKLLGPVGSIAGYFIALVGIIQLFSSFLAIVLIIIGL
                     FIGFTSFAAQVDFGNKRFRNALLLFGIIPVGKWQSIDSSMGLSVKRLNRAWRTYSSSN
                     RSFDIADSDYRIVIVDKQVNPLADIQRVQSLEKANETLVYYSEKLGLPIVKQGS"
     gene            37381..37803
                     /locus_tag="PLW22_00555"
     CDS             37381..37803
                     /locus_tag="PLW22_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOU73219.1"
                     /translation="MKTQVDISHRISPKLFWFVTLMIITLPITTQYRLLIFGEYTTGE
                     VVGVEYEKTLGISAFGPDKYPIIVFTTLEGKSVQFYGLENFEYPKGTILKVVYSPNNP
                     TDCRVFSFGALYTDRKAIIPGILFLLWMAFYLAFRNKK"
     gene            37853..40321
                     /locus_tag="PLW22_00560"
     CDS             37853..40321
                     /locus_tag="PLW22_00560"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOU73220.1"
                     /translation="MINLTAIQLAEIVLGQVIGDSGFTIRNVITDSRVPVSGNGSMFI
                     ALVGPNHNGHEFIGELYNIRNIKAYLVEKGEVDYTNYPNATFVEVDDTLQALQKLAAY
                     HRNRFVYPVIGITGSNGKTIVKEWLNQLLFSEYQIVRSPRSYNSQVGVPLSVLQMDNH
                     FDLAIFEAGISKPGEMVKLQPIIDPTIGIFTNIGMPHQENFDDIDEKVNEKLKLFREV
                     KTLIYCKDHAVIDAVVQDNTLLADNQLLTWGQNKESKVQIMSVKREAGKTLVHVRFNN
                     HEHTITIPFTDAASFENAMHCVATMLLLGYSLQDIDTRLQRLQPVAMRLELKEGINDC
                     SVINDSYNSDLGSLAIAIDFLMQQKQHERHVVILSDILESGYAPEQLYSKVAQMLEDK
                     GVDLLIGIGPEISRYDYAFEIEAEFYSSTHEFLLDLKRKEIRDAAILVKGSRKFHFEQ
                     ISAALERKVHRTTLEINLNAIAHNLNYYRSLLNPGVKVMAMVKAFSYGSGSYEIANLL
                     QFQRVDYLGVAYTDEGVSLREAGISLPIVVLNPSFGTYELMVEFNLEPEIYNITGLKE
                     FINVLNRVGVSSYGIHIKIDSGMHRLGFTPDQLDELIAILKSTNSVRVKSIFSHLAAS
                     DEPHHDQFTIHQIETFHKACNVLSDVLGYQPIRHILNTGGIERYPEAQFEMVRLGIGL
                     YGISPTHGDKLMPISTLRSKVIQVKQIKPGDTVGYSRKGIVDTPTTVVTIPVGYADGL
                     SRMLGNGNYSMMLNGTLVPTIGNISMDTCTLNANGVEVKEGDEVIVFGNKPTITDMAK
                     AMETIPYEVLTSISQRVKRVYFQE"
     gene            complement(40323..41030)
                     /locus_tag="PLW22_00565"
     CDS             complement(40323..41030)
                     /locus_tag="PLW22_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU73221.1"
                     /translation="MSRNSYNIDIESVFEGVCGLFPNLKPEEREIMRNNITCQYFRKG
                     EIIYKEGDTPQGLVCLSKGKVKLYKEGVGGRDQIVRMAKPIGFIGYRALFAEENYSAT
                     AEALEDSVVCIIEKNAFMGILRNNAELTLIILKSVATELGFSTNRTVTLTQKHIRGRL
                     AESLIFLRDTYGLEDDGKTIKVYLSREDLANLSNMTTSNAIRTLSGFAEEGVIELDGR
                     RIKILDHHRLERISDLG"
     gene            complement(41125..42792)
                     /locus_tag="PLW22_00570"
     CDS             complement(41125..42792)
                     /locus_tag="PLW22_00570"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOU73222.1"
                     /translation="MKVALAQLNFTIGHFDSNADKIIASINRAKAEGARLVVFSELSV
                     CGYPPHDLLTHKHFVDKSIATVNQIAQYCNGIYAIVGAPSINTDTTGKLLFNSAYLLG
                     EGKVLDAYHKTLLPDYDIFDEYRYFEPNPGNYRVAEIDGIKIAITICEDIWDDQPTTT
                     AFSRSKLYRDAPMNHLVKLNPDMVINISASPFSYNVEERRLQVFQSNVARYSKPVIYV
                     NQVGGNTDLLFDGGSMVVNAGGDIIEKLKSFEEDFRVIDTNVLERNQSIPPEISATSD
                     QRIERIHNALVMGIRDYFGKLGFTKATLGLSGGIDSAVVLALAAQALDVNNIRVLLMP
                     SQYSSQHSIDDAVELAQKLGVQYDIVSIQPIFESFKNQLSSIFEGRPEDITEENIQAR
                     VRGTLLMAISNKFGHLLLNTSNKSEAAVGYGTLYGDMCGALSVLGDVYKTDVYRLANY
                     INRNDEIIPINTIQKPPSAELRPNQKDSDSLPPYEILDPILFAYIEQGLSAEEIVGLG
                     FPSDTVNRTLRLVNMNEYKRYQSPPILRVSSKAFGYGRKMPLVTKWS"
     gene            complement(42811..43476)
                     /locus_tag="PLW22_00575"
     CDS             complement(42811..43476)
                     /locus_tag="PLW22_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU73223.1"
                     /translation="MKLNSLTLLIGLILLGNQLANAQQYSFGVFADPQLSWFNSDTKR
                     YDPNGPVVGLNIGFTADKFFAKRYAFSSGLSVNTLGGNIRLMQSTYTLKTIDGTYNIA
                     IGDNIKFKSQYLTLPIGFKFRTNQIGYTTYFANVGVSGSLRIRSYAWNETNHVDRETT
                     KTDMAWGFASYQIGIGGEYSLGGESAILFGITWCDGLTRIIELPNSTITSQSLSLKVG
                     IIF"
     gene            43641..44462
                     /gene="ispE"
                     /locus_tag="PLW22_00580"
     CDS             43641..44462
                     /gene="ispE"
                     /locus_tag="PLW22_00580"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOU73224.1"
                     /translation="MILFPNAKVNLGLNIVRKRPDGYHDIETLFYPVKGLCDILEVVA
                     TDGEGNTIEFTQTGIGIDCDTENNLCVKAYRLLAQRYGMPHVRMHLHKLIPIGAGLGG
                     GSADAAFALRALNTFLDKPLSHENLLSMTLELGSDCPFFVHNTPAIGRGRGEQLEPIS
                     INLTGYWILLVNPGIHVSTREAYEGSNPKPWKNPIGNIVNLPVSDWPNKLVNDFEKTV
                     FPKYPLIERVKEELYSMGAVYASMSGSGSTVFGIFKSKPVITDAFKGYFTHTSEM"
     gene            complement(44592..45092)
                     /locus_tag="PLW22_00585"
     CDS             complement(44592..45092)
                     /locus_tag="PLW22_00585"
                     /inference="COORDINATES: protein motif:HMM:NF025725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HOU73225.1"
                     /translation="MPVYVLDSNFFIQAHRFHYPIDVAAGFWNKVRQLAEEGRVISID
                     KVKKELYDRNDALEEWCRNNLPEDFFKDTSVVMTAYAQVTAWAMSRSGHYLPNALNEF
                     LNADEADAFLVAYCLADNANRFVVTQEVSEPNRQNKVKIPDACIALNVSYVNTIEMFR
                     QLGETF"
     gene            complement(45098..46228)
                     /locus_tag="PLW22_00590"
     CDS             complement(45098..46228)
                     /locus_tag="PLW22_00590"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOU73226.1"
                     /translation="MANEINVNTNMLTWAITRAGYDVPTFAVKFPKILEWLEGQKKPT
                     VKQLEEFSKKVYLPFGYLFLPQPPQEKLPIPYFRTNGNQADRISINVFDTILLLQQRQ
                     DWLRNYLKDNDFHPLPFVGKFRNSNNVNAIVADIRQTLELTENWASQFRTWQDAQDHL
                     VLHIEDKGIITIFNGIVENNTHRPIPVDECRGFVLVDEYAPFMFVNNSDFKSAQMFTI
                     VHELAHIWTGHSAGFDFRRLQPANDPIEILCDKVAAEFLVPAQEFDEVWNHKPNNFAY
                     ASRYFKVSEIVIARRALDTGKISRPQFFDFYEEYSNREFAKKESQGSGGDFYATTKKR
                     ISITFASHVNQAVKSGKLLYRDAYKLTGLKGDTFENFFSKHL"
     gene            complement(46242..47456)
                     /locus_tag="PLW22_00595"
     CDS             complement(46242..47456)
                     /locus_tag="PLW22_00595"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOU73227.1"
                     /translation="MKKELLGDRVELIYGFSVRTDNEPKGKYPVYGSNGIIGFIDSFK
                     VKGPGIIIGRKGTVGAVTYSSENFTPTDTTYYVALKDNTKDDLKFWYYYLQLIGLDKL
                     NSHSTVPGLSRDLAYLKEIRVPEKAEQKLIADFLTVIDDKIALNKRINTELENLAKTI
                     YNYWFVQFDFPNNEGKPYKSSGGEMEWNEELKMEIPKGWKTKSLYDIAIYTNGLAMQK
                     HRPNGDEKLPVIKIKEMNEGYSAATEYARPDIEDAYLIREGDVLFSWSASLNVMIWAD
                     KVGALNQHIFKVTSEFYPRYYFYHQISNYLNHFKLMAENRKTTMGHITIDHLKESRIV
                     VPDDDSLILKCGERIEPILDLIVKNRMTNIDLMEVRDFLLPMLMNGQVKVKSEAKEQL
                     SMAAEPQVKYGK"
     gene            complement(47453..49096)
                     /locus_tag="PLW22_00600"
     CDS             complement(47453..49096)
                     /locus_tag="PLW22_00600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOU73228.1"
                     /translation="MTKHLEYTQKTKDLIDGLKTVCSNYGLANSGNEYKIITEVFLYK
                     FLNDKFLHEARKANKDLKNSTNIETDIQKMSNSDYEWMLEKIGERSAKLQKHHFISYL
                     FNKQNEPNFHELFDKTLLEIADLNIKLYSVSTIDKERVRLFDRISPYIIESNQKTPFC
                     KAIINKLVAFSFEPIFEEKYDFFSTIFEYLIKDYNKDSGQYAEYYTPPAVAKIMAKIL
                     VPEPTRNVTVYDPAAGSGSLLLALAHQIGENKCTIYSQDISQKSSEFLRLNLILNNLV
                     HSLHNVIKGNTLTNPYHVNDAKNDLKKFDYIVSNPPFKMDFSDDRETLASDQHRKRFF
                     AGVPSVPKKKKESMAIYQLFIQHILYSLNDKGKAAIVVPTGFCTEKAAIALAIRKKIV
                     DSNWLHAVVQMPPNIFATTGTNVSVIFIDKSKTRDTAILVDASKLGTKVKDGKNQKTL
                     LSNEDEKRIIDTIKGYRQLDDFSVIVNNEQMKQKAYSFGAGQYFETKIEYVELSPEEF
                     EERINEFKNKISFYFNNSRELEIQILKNFNKLDYKKNNK"
     gene            complement(49504..49965)
                     /gene="tnpA"
                     /locus_tag="PLW22_00605"
     CDS             complement(49504..49965)
                     /gene="tnpA"
                     /locus_tag="PLW22_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOU73229.1"
                     /translation="MPGTFSQIYIQIIFAVKGRGSLIYSDWEERLYQYITGIVRGKEQ
                     KMIAINGMPDHIHIFIGMKPSCCLSDLVREIKKASNEFINENKLSNFRFSWQEGYGAF
                     SYSHSQIDNVVKYIMNQKQHHRKVSFREEYVDFLKKFEIEHDDKFLFEWVK"
     gene            complement(49968..53051)
                     /locus_tag="PLW22_00610"
     CDS             complement(49968..53051)
                     /locus_tag="PLW22_00610"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOU73230.1"
                     /translation="MTFNEDSRVKIPAIVHLTRLGFTYVPKSGITNLHADTNIFKDLF
                     KQGLSKINGTEYSDSEIETFLSEINIQLDNSDLGKAFYKSLQGDFACKLIDLENFDNN
                     LFHVVTELTYKNDEEEFRPDIILLINGMPLAFIEVKKPNNREGILAERNRINTRFKNP
                     KFKKFMNITQLLVFSNNQEYDEENITPIQGAFYATPDPEQVNFNCFREEDNSLIHNVP
                     QEDSEIEKQILLDNNIVSILGTKEYETAKGPNSPTNRILTSLFSRQRLKILLRYGFAY
                     VNTVNNGITKIEKHIMRYPQLFATLAIEKKLIAGVKKGIIWHTQGSGKTALAYYNVNY
                     LKDYYQKQNVIAKFYFVVDRLDLATQAKNEFEARGLKVDMVSSKEDFIKNIKTAGATS
                     GNTGAQTITVVNIHKFSAESISKVSDYDLNIQRIYFLDEVHRSYNPQGSFLSNLIASD
                     RNAVLIGLTGTPIISKEYKSKDIFGDYIHKYFYNQSIADGYTLKLIREAIETKFKAEI
                     NQVYDEIVKQGEFTKSQIFAHPKFVEPLVDYIITDFKKSRVLHNDQSIGGMIVCDTSD
                     QAKMIFTEIQKYNKRLEAQQTVYHEPQTDYYIAAESGVGYLTPELAPISIALILHDID
                     TKEIRKEHQTDFKQGKIDLLVVYNMLLTGFDAKRLKKLYLTRVVKEHNLLQTLTRVNR
                     PYKNFKYGYVVDFADIRKEFDKTNKEYFKELQEELGDETKNYSNLFKSAEEIDKEIQE
                     IKEKLFLYDFSNIEEFQQIVSQITDKKEIIELKKCLENLKSLYNIIKIMGYTELLDKF
                     SFDRVNKLYNEVANRIDILNLKENLENAADNTNLLNLALENMQFTFRKIATHELQIAD
                     KFRSELERTRKELERNFDKKDPKFISLFEELKRLFKKKNIEELTSEEMDEAINDLKKI
                     YEQANALNNKDAMLAAKYENDIKFARIHKRIKEQNMNVLNSDLALHETLIFIKHETDK
                     TVVNNQAILNNPDYFAEITKRTILETLENKGIRDLNVVRFFNNAVVNEYFYQRAI"
     gene            complement(53475..53708)
                     /locus_tag="PLW22_00615"
     CDS             complement(53475..53708)
                     /locus_tag="PLW22_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOU73231.1"
                     /translation="MIHPKLDDKNVTAFIPGTIIDVKVKKGQEVKQGDVLVILDAMKM
                     HNRLIAPSAGKVKEVKVKPGDRVSKGALLVSIE"
     gene            complement(53750..55294)
                     /locus_tag="PLW22_00620"
     CDS             complement(53750..55294)
                     /locus_tag="PLW22_00620"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOU73232.1"
                     /translation="MPLKNKIEELKKRKEEVLQGGGEQAVAKQVAMGKLTARERIIAL
                     LDEDSFNEYDMFVEHDARDFGMDGKVLHGDGVIIGTGTIFGAPVAVYAQDFTVAGGSL
                     GSMHARKITKIMDYALRMRIPLIGINDSGGARIQEGVNSLAGYGEIFWRNTLSSGIIP
                     QISVILGPCAGGAVYSPALTDFVFVVDKISKMFITGPEVIKSVLGEEISQEDLGGAKV
                     HAQITGNAHFYAESEYECFEQIKKLITYIPWNNTRKARRFEPKEPKVNFNIEDIVPGD
                     PKEPYDVRDVIRAIADDSDFFEIQEKWAANIVIGFGRLNGETVGFVANQPLVMAGVLD
                     VDSSDKAARFVRYCDAFNIPLVTLVDLPGYLPGIDQEHAGVIRHGAKLLYAYSEATVP
                     KMTVILRKAYGGGYIAMSSRHLRADFVFAWPSAEIAVMGPEGAANIIFRKEISEAPDP
                     EAMRRQKVEEYKEKFANPYVAAAKGYIDTVIEPAETRKLLLHALEVSSTKVADRPFKK
                     HGIPPF"
     gene            complement(55470..56546)
                     /locus_tag="PLW22_00625"
     CDS             complement(55470..56546)
                     /locus_tag="PLW22_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU73233.1"
                     /translation="MKLLDWYIIRKFIGTYFYAIILIVGIAVIFDLAEKIDDFLEKDA
                     PMYDIIFRYYLNFIPYFANLFSSLFVFIAVIFFTSKMAYNTEIIAILSSGVSFKRMLY
                     PYFISATIIAIFSFVLNSFIIPPANAVRLDFENKYIKRPYVNTDRNIHRQVRPGMFVF
                     MRSFNTYNNTAYNLSIEKFDGNKLVSKLIADNAQWDSTKEKWTINRYYIRNYTDSGEE
                     IIKGDKIDSTFYLTPSDFNQRLKIIETMNLFQLNDFIAEQKLQGADTIEYLLIEKHGR
                     FAYPFSVFILTLIGVSLSSRKVRGGIGLHIGLGLALSFSYILFMRFSTMFAVGGHLPP
                     AISVWVPNFLFAIIAVGLYRMAPK"
     gene            complement(56556..57686)
                     /gene="tgt"
                     /locus_tag="PLW22_00630"
     CDS             complement(56556..57686)
                     /gene="tgt"
                     /locus_tag="PLW22_00630"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU73234.1"
                     /translation="MDFKLLKTDSQTSARAGIITTDHGKIETPIFMPVGTVGSVKGVS
                     HRELENDINAQIILGNTYHLYLRPGLDVLKQAGGLHEFSSWNRPILTDSGGYQVFSLA
                     DMRKLTAQGASFRSHIDGSPHLFTPENVMDIQRIIGADIVMAFDECPPGNADYEYARK
                     SLDLTEKWLERCVQRFDTTEPLYGHSQTLFPIVQGCVYPDLRKRAAENVQKYNRDGYA
                     IGGLSVGEPAEVMYQMVEVVNGILPKSKPRYLMGVGTPENILQSIALGIDMFDCVMPT
                     RNGRNGMLFTSEGIINIRNKKWQADFSPIDPFGLTFVDSRYTKAYLRHLFIAGEMLGP
                     QIASIHNLGFYLWLVGEARKHIVEGDFLQWKNAMVKKVSKRL"
     gene            complement(57778..58563)
                     /gene="truA"
                     /locus_tag="PLW22_00635"
     CDS             complement(57778..58563)
                     /gene="truA"
                     /locus_tag="PLW22_00635"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOU73235.1"
                     /translation="MPRFKLTIEYDGSRYRGWQFQREAATVMGKLMDAIKEVYKVSSY
                     ELYGAGRTDAGVHALGQVAHLDIDSAMPPYVAMERMNEVLPASINILDMAKVSPRFHA
                     RYDAKHRSYVYHISRRRTAFGKDYCWWVKEPLNVEAMAAVTHHFTGMKDYKSFGAPEK
                     EGESTLVKIDHLKIFEVDDSILIHIVGSHFLWKQVRRMVGTLVQVGLGKLSEADIDSF
                     FSEYSTLPAKFTAPPSGLYLEHVYYAGESINQEPTWLINIGKY"
     gene            complement(58620..58719)
                     /gene="ffs"
                     /locus_tag="PLW22_00640"
     ncRNA           complement(58620..58719)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLW22_00640"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            58817..59362
                     /locus_tag="PLW22_00645"
     CDS             58817..59362
                     /locus_tag="PLW22_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOU73236.1"
                     /translation="MEKQGNPMLKHSMNYGLIMGVALIILSLITYLAGIVKPPFWVSI
                     INYAIIIGIIVWGTKTYRDEVLEGAITYGNALGFGVLITLFAGVIVAVFTYIQITLID
                     PDFVSKMLAIAEEELVKRGMPDEQIEMAVEMQKKFMTPLIMTISSLLGMVLMGFIFSL
                     ITSIFLKKEKSPFAGGGEVIE"
     gene            59378..>59889
                     /locus_tag="PLW22_00650"
     CDS             59378..>59889
                     /locus_tag="PLW22_00650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU73237.1"
                     /translation="MDISIVVPLYNEEESLPELMEWIAQVMTQNHFTYEVIMVDDGSN
                     DNSWAVIESLRLKFPSLRGIKFLRNYGKSAALQVGFTAAQGDVVFTMDADLQDSPDEI
                     PELYRMVKEQGYDLVSGWKKKRFDPLLTKRIPSKLYNATVRMVSGIKLHDMNCGLKAY
                     RRNVVKSIEV"
BASE COUNT        16054 a        12461 c        13252 g        18122 t
ORIGIN      
        1 taaagtaaaa attattattt tcaaatttca atgtaaagtg agtatttcca tttggatatt
       61 tccatgtcca aattcctatt gggatgtcat ttttgaattc tcccttaatc cattcgatgc
      121 ctgactcata atagaaaata aataaaccgc tttttatgtt attcacataa ttaccttcaa
      181 caatcttttt cccatttatt aaataatcat ggaattcacc atcaaaataa tttttatcgg
      241 tattccagtt acatattcta tagtaacttg cagtgctctc tttgcaaatt ttccactctt
      301 tatcataaaa tatttttact ttgattgttt cacctgatgc aaaattcatg ataaagattg
      361 agagaaatga aagtaatgtt tgtttcattg ttctaataaa ttattttaga cttttattat
      421 ttttagttga tttacaaaac ttatcaatgt aaacattagc attcttgttc ccgagtttta
      481 gggacttact ccagtcttcg caagctttgt ctatagttcc ctttgcgtaa taacttattc
      541 ccctattata gtatgtattg gaatgattgg gttttaattt tattgaatta gtataatcta
      601 aaatggctga atcaatctga tttaatttta aattgcaatt tcctctgttt aagtaataca
      661 agtatattaa aggaaaattt tcaccattat ttttgctaag attaatagct ttactgtatt
      721 ctatttttgc tttttcatac tcagatttaa ttctataaat ttctgcaata taaaaagtta
      781 attcgtcgtc atcgttgtaa atgtttcttc ccttaattaa aaagggtaaa actttgtctg
      841 caaataggtt atctaatgtg aatattttga ataaatacaa atatgctggt ctgaatgtag
      901 agtcaattgt tatcgattcc aaaatagtta tgtatgcttt ttctaactcg ttattttcta
      961 taaaagtact tgcctgtgaa attaattcta tagccctatt tctgtcatta acctggacta
     1021 tataatcctg agagaatgat ttttctgtaa ttagaattaa aaaaattgct atgtataata
     1081 tttttttcat aataattaca acgtaataaa aaattgcatg tcccagcaga gttattaaat
     1141 caggttatga ttaataaact catttgcctg aactttgata atcttccctg agcaccctaa
     1201 actcagttct tcggttgatt tgatgaactg tttctttttg atcctcactg gttaaggagt
     1261 cgataaattt cttatcaaga acagttcctg gtgttaggaa tgggtattga gcagcaaggt
     1321 tatcgtcaac aacctttggt tgggtttggg cgtagccttt ggctttcaac ctatcggttg
     1381 ctatgccgtt ttcaatcaaa tagtttacta ccgcctgagc acgcttttgc gatagctcat
     1441 agttatactc ctgagagccc ctactgtcgg tatgcgatgc cagctctatg gttatgttgg
     1501 ggttgtcgtt cagtatctgg ataaggttat caagcgcttc ttttgactcg ggacgaagtt
     1561 cccacttgtc gtaatcgtag aaaatgttgg gtatttcaat tggcttagcg gttgaggtta
     1621 aggctattga aacgttaaaa tcctggctct tggttaaccc cttagtggtt tccttcgcct
     1681 tactgtttag gtatcccttc tttgaagcaa caattacgta atcggtgttg gggacaaggt
     1741 taaacctgaa tgaaccatcg ctctgggtct gcatggttgc aatggtgcca tcgctgccaa
     1801 ccagtttaac ggtagcatcg gtgattgtag ttcttgtttt ttcatccatt accttgccta
     1861 tcatgttaaa gtttataggc gggaggtaga acatgtagat atcgtcagct ccacggccac
     1921 cgttacgccg tgaggagaag aagccagctt cgcgttcctt ttcgaaaata ataccaaagt
     1981 catcggcagt tgagtttacg gggtagcgca tgttctctat tacccattgg ccttcatcgt
     2041 ttttctttgc cttaaatatg tcgaggccgc ccatgccagg atgtccatcg gaagagaagt
     2101 aaagggtgcc atcgggatga atgtatggaa acatctcgtt tccgggagtg ttgatttgtg
     2161 ggccaaggtt aataggcgcg ctccactctt cggaacccgc ttcgcgggta attttccaaa
     2221 tatcgagtcc cccgtttcct ccgggaatgt tacttacaaa gtaaagttca gttccatcgg
     2281 ccgaaatggc tggatgggca acaatcatac tatcggcagc cagttccaac tctttagggg
     2341 taagccaacc ttggtcgctt tgcttggcgt aaagtatctg gcatccaagc ttgttacgct
     2401 tgctaacctt acaacgggta aaaaacaagt tgttgccatc gggagttaat gagggggtgc
     2461 cctcgtcgaa ttgcgagttt atggtttcat ccagaggttt tggggcactc caaacccctt
     2521 tattgtcgag ctcagtgata aaaatgtcgg aaaaattctc gccggttacg gcgcttgtct
     2581 tgtttccgat agtaccgttg cgggagctgg taaggtatag aatattgtag tcggttgaag
     2641 cgtatgctgg tgagaaatcg ctaaactttg agttaatggc gcgcatgttg gttatttcat
     2701 aaccagccgg tgtggcaatc cactgcttgg caagttcaca cgattgtatt ccgacctcgg
     2761 caaggggatc gttagggaca agaactttgt acttttggta ctgttcaatg gcaaggtcgt
     2821 acttttcatt catcttcagc atttcagcgt agtagtagta aaccttaggg tttgggcatt
     2881 cccttcgaat ggctttttcg taccactgct cggcctggcg gggttctcct atttgtctaa
     2941 aacagtttcc tattttaaaa aggtagaggg aaagttcctt tttatcttta atcttgtcga
     3001 tatcgtcgcg gtaaagggca atcgcatcaa agtatgcacc ggcattgtat agcgcatcgg
     3061 ctttctctaa ctttttagtg ggttgagccg aaatgtttcc tgaggttagg taaactaatc
     3121 ccaatgcaat aagaagtagt cgcttcattg tgctttaaag gttttagtca caaaattatg
     3181 gtaaatataa gtaaatgcca attaagtagt aacgcaaggg ctattttttt attataactt
     3241 attcacaatg gtttttcggc ttagtagggt agatgcttac tttgctcgcc catttaaaag
     3301 gatgacctta tttaatgttg attagggtta gtatccaaat acattaatca ttcaatcaca
     3361 agagtgtcaa aactacatgt gcaattaagt acgccccgct ttaaagagcg gggcgtatgg
     3421 aaaacaaaaa agttttagca gttgaatgct attaccgaat gaatagcatc tccctatact
     3481 tgggcaaagg ccacatctca tcgtcaacaa tcatttccag cttatcgata tgcgtgcgaa
     3541 tgctttcaat atagggttta acgtttttcc cgtacaagtg ggctttttca acaatatctt
     3601 caacctggtt ggcttccttt cggcgttcaa tcatctcgtt aacctgatga cttatggcat
     3661 ggatatgttc agaaatttcc aggattaaag catgctgatg atcggccagt ttttcccatt
     3721 catttgggaa aagctccttt aatcccttaa cgttgctaat taaggtgttt tggtatgcaa
     3781 tggcagtggg gataatatgg ttgatagcta aatcgccaat cacgcgggct tcaatttgta
     3841 ccttttttac aaagatctcg ttccttattt cgtaccgtgc ttcaagctcg cgttttgtta
     3901 gtattccgtt accggtgagg agttttacag aggagtcgag caggaaagtc ttgaatgcct
     3961 ccagtgggtt gctgatgtta ggaagccctc tgcgtgctgc ctcgtcaacc cattcctggc
     4021 tgtaaccgtt gccctcaaag cgaatctttt ttgactcaat aatcaccttg cgcaggcact
     4081 ggattatggc ttcgtccttt ttaattcctt tggcaattac cttatcaacc tcatctttga
     4141 acttcatgag ctgattggcc acggctgtgt taagcacaat cattggagat gcgcaactgc
     4201 ccgaggagcc tacagcgcga aactcgaatc ggtttccggt aaaggcaaat ggcgatgtac
     4261 ggttgcggtc ggtattgtct aaaaggattt ccgggatttt accaatgatt tccagtttga
     4321 gttcggtctt ttcgtcggga gtcatttttt ggtctgtcac cttttgctcc agctcgtcga
     4381 gcacggcact tagggttttg ccgatgaaca ctgacatgat ggctggcggt gcctcgtgcg
     4441 caccaagtcg gtgggagttt ggttccgatg ctatactacc caaaagtaat gcggagtggt
     4501 catgtacggc tttaatggta ttgatcagga aggtcaggaa ttgtaggttg ttctttggtg
     4561 ttttgcccgg tgaaagcagg ttagtcccag tatcggtcat cattgaccag ttgttatgct
     4621 tgcctgaacc atttatcccc ttgaagggtt tctcgtgaaa tagaacacgg aagccgtgct
     4681 gacgagcaat gcgatccatc agcgacatga taagctggtt gtgatcgaca gccaggttgg
     4741 cctcctcaaa aatgggtgcg cactcaaact ggtttggcgc cacctcgtta tggcgggttt
     4801 taataggtat gccaagctta agtgcttcaa cctcaaaatg acgcataaag gcggaaaccc
     4861 ttgcgggaat tgaaccaaag taatggtcct caagctgttg atccttagct gcagcgtgtc
     4921 ccataagcgt tctgccacac aatgcaaggt cgggccgggc gttatacagc gattcatcaa
     4981 tcagaaaata ctcctgctcc cagccaagcg tggcataaac tttacgggca tccttatcaa
     5041 agtactccag caccgaaagg gctgccttat cgagtagtgc aagcgatttg agcaatggtg
     5101 ttttaaagtc gagtgcctcc ccggtatacg aaacaaacac agtgggaata catagggtat
     5161 tctctagtat aaaggctggt gaggttggat cccaagcggt gtatccacgg gcctcaaaag
     5221 tattacggat gcccccgctt ggaaaacttg aagcatcggg ttcttgctga acaagcatat
     5281 cgcctttaaa aacctcaatg ggttgacctt tgatatcaat tgagaggaat gcgtcatgct
     5341 tttcggcggt ggatccgttt agcggatgga accagtgtgt gtagtgggtt gccccacgct
     5401 caatagccca ggccttcatg gccgaggcga cctgaccggc aatcttacgg tcaatctttt
     5461 taccctcatc aatggaggtg ataatactct cataagcttc ttccgatagg tacttcttca
     5521 ttttaggcct atcgaataca ttgatggcaa agatatcgac aagcgattct gctttgccct
     5581 cgccgttcat gtcaaccggg atgcgattag ttgcttcacc gaatgctcta aatcttagtt
     5641 ttggcatagt gtggtatgtt tttcaagggc aaatataaga aaaaaaatca aaataaatag
     5701 tttagatata taaaaaatag gggtatgtat gcagaaaaaa taaaaatatt taatatgtat
     5761 aaaaataaac agggggtgtg atgtaaaaaa taagtatttt gttaaatggt tgtaggttta
     5821 aaattcttat caggaatcgt cccttgtttt gatgtcagct ttgtatttgt ttcttggtag
     5881 cgggttgctt gccttagtgg tttattgtga acacatttat ccattatgta tcaccttggg
     5941 ttggtttttg aacaaagtgt tgccgttttg cgttggtttt tgtaaattgc aagcatggaa
     6001 agcgataaca gaaaatattc caacgacgaa atcactgtgt tctggcgacc gggagagtgt
     6061 gtacatgcct ccatttgtta cactaagctg ctctcagtgt ttaacccccg taaacgacct
     6121 tgggttaaca tgaacggtgc cagcacagag cgaattattg agattgtgaa tgagtgccca
     6181 acaaacgcac ttacctttat gtggaacgat cctgaaaaaa ataagcaaga gcgttcgcac
     6241 aaatgtgttc gggaatttac tcccgaggag gttgaggcgt ttgaggttac gccggtaaag
     6301 attcaggtaa tgcccaatgg cccattgttg gtttcaggcg attttcagat tattgatgcc
     6361 gatggcaagg aggttaagtc aatgaaaatg gtttccattt gccgctgcgg tcattcaaac
     6421 agccagccat tttgcgatgg cacacatttc aaaaaaggat tccgtgatag tgagtagcaa
     6481 aatgaaccag ccaaaaaaaa ttgcagcaaa gctgcatcta agcaaaggag gcccaattag
     6541 ggttgagggg cgcttcttta ttgttaacct cgatggtgaa agtcttatac ccgataactc
     6601 caaggaagtt ttcctatgca cctgtgggtt atcgcgcaat aaaccctttt gcgatggtag
     6661 ccataaggga ggaaaatcca atgggtaggg ttatttcgtg cctagtgtat ctgtccgtat
     6721 ggcaataagc tagaataatc aaaataatta tcatttaact taattagcca gttgcgcctg
     6781 aacacaattg ctatctttgc atggcataag ttttaaacgg gcactaactt ggaattaaat
     6841 aaagcaagaa aagcgaaaag ccctgcgata agggctttgt ttgtgatatt cggcatagta
     6901 tcatttgggt tgggagtgtt gggcattttt ttaccattac tacccacaac accctttatg
     6961 ctgcttagcg catttctgtt cattcggagc tcgggtaggc tttaccggtg gttaataaac
     7021 catcggcttt tcggaaaata catacacgat tacattgaac gccggtcaat tccacgacga
     7081 gtgaaatggt atactttagg attgctctgg gcatctatac ttacaagtgt tgcaacactt
     7141 gatgttggca acgtcatccg gttaatacta ctgttaattg cggttggtgt taccgcccat
     7201 gtggtaaagt tacgaaacag ctagccttcg ctactccctg ctacgattaa gggcatcgta
     7261 gatgaccagt gtgaagaatt ggcgcatgtt cccacccatg gccttaacct gctgggggat
     7321 aatacttgcc tcgctcatgc cgggcacggt atttatctca aggaaataga ttttgtcatt
     7381 attaacaata taatccacac gaaccacacc gtggcattgc aatcgatcgt atatctttga
     7441 tgtgaattgt tgtatggcac tggttaacga ttctgggatt ggggcagggg taatctcctt
     7501 cgacttgttt cggtattttg cctcgtaatc gaaaaactcg gtttcaggga ctatctcggt
     7561 tacgggcata atcatttctc cattctggta tttgaaaaca ccgcaggtga attctcggcc
     7621 gggtatgaac tcctccagta taatggtttt atcttcggca aatgcttttt caatggcggg
     7681 cattaagtca gaggcctgct taaccttgct tactccgtag ctgctgccgc tctcattggg
     7741 tttgacaaaa atgggtaatc ccaaagtgtc cagaattgct tgcgcatcta ctttttgacc
     7801 gcgcctgagc agcaatccct ttgcgaggtt aactcctgtt tcgcgaacta actccttgca
     7861 gtaatacttg ttaaaggtaa cggaagagtt cataactcct cctgtggtgt agggaatttg
     7921 aagcatctca aagtatgcct gaagcagtcc gttctcgccg ggagtgccat gtatggctat
     7981 cagggcgcag tggaacgata gtttttgtcc atcaatatca acggtgaaat cgtttttatt
     8041 gacaggtatt tgacctttct gggggtgttc aagtgtccag gtagtacctt ttacgagtat
     8101 tttgtatgca ttgaattgtt cggggtcgag ccaaccggca atctgggtgg cgctacgcat
     8161 ggatatgttg gcctccgatg agttacctcc agccattatg gcgatgttga gctttgtgtt
     8221 catagtgttt taaattcatt gcaaagctgc aagaattttg gcaaaaataa aagagtcgca
     8281 ggcggaaact gcgactcttt ttgaagtaat cttttgtttt tatcgggcat cggagaagta
     8341 acgcatgaac tgtgctctct cgtatgccag cggttttatg ctatcggctt ggcgtagctt
     8401 gccagtaatg gaattaatgg attcaaaatc attggcatct agccatttac tggtttcctc
     8461 aatccatggt tcaagtatct cggggcccga tttgtacata gccgaagcca cctgaaccgc
     8521 attggccccc aagaggatat tcttgaccgc tgtttgccag tcgtgtatgc cggttgaggc
     8581 tgccaggttg catgcaactc gccctgaaag aatggaaagc catcggcgaa cctctgcatt
     8641 ttcatcggga tggctgagta tgctgcctga ggtaacctta agagtgttca ggtctacatc
     8701 gggttggtaa aaccggttga aaaggacaag tgccttaatt ccggtccggg atagctccac
     8761 ggcaaagttt gccatgcctg aaaagtagtg atgtattttc atggcaatgg gaaccgatag
     8821 tgtttgtgct accttgcggg caacatcgat gtaaattttc tcaatctcat cgcctttcat
     8881 cttaacatcc gaaggcatta taaacatgtt gagttcaatg gcatcggcac cggcggcttg
     8941 aatatcgcct gcaaagtcaa tccagctatc ggcgctacta cagttgatgc ttgcaattac
     9001 cggtatactt agcttttgct tcatgccatc aattagttca acatagttgc taagggcatg
     9061 cttgcgggtg tagtatgcaa tgtagtcctc ggcttcgctg taggagttga acatgttgtt
     9121 catgcgttgg gcatcaacat ccatcatgat ttgctcttca aagagcgatt taaccacaat
     9181 ggcggcaaat ccatgtttct ggagcagggc tgctttttca accgagtcgg taagtccaga
     9241 gctcgatgca atgtatgggt tcttcagctt gaagcccatg tatgtggttg ataggtctgc
     9301 catatcgttt taagtttaag ttgattgact tcttttactt tttgccaccc aggaacctat
     9361 cgattccaat ggctgcttta tggccgtttg caataccatg tataacatcg gggccctgaa
     9421 tgatatcgcc acccacaaag agccattcga cgcttgactg gaagtagtca ttaactacta
     9481 ttcgtccgcg gctgtcgagt ttaagattat ccttgatgct ctcgggtagg tagcttaaat
     9541 ccattccctg tccaatggac tcaaccacca tttcgccggc aaagaaatcg gtttgggtgt
     9601 catcaaactt ggggttgaaa cggcgattct catcgaacac tgaaacgcac ttaactacgt
     9661 gtaagccttt tattttgttg ttttcaattt cgattttaac gggtccccat ccgggaatga
     9721 ttgtggcacc ttcctcgcgg gcctcaacaa cttcctcgcg gtcagctggc atctcatttt
     9781 ccgattccag cgaggtggtt atgatattca ccttgccata cctctggttc tggattcggg
     9841 caagtgttcg ggtgatatcc atggctacgt taccgccacc aataaccaca atactcgggg
     9901 cagcaggaat ttccttgccc atggttacat cgcggagcag gtcaacagcc tggtaaacat
     9961 ggggatggtc agtaccggga atgcgggtgc tacgaccaag gtgtaagcct gttccggtaa
    10021 acacagcgtc gaactggttg tgcagttcct ccagagtgat atccttaccc acggtagtat
    10081 tgcaccggat ttcaacgccc agcgatttga tataatcaat gtccttgtca agggcatcgt
    10141 agggtagacg gtactcagga attccatagc gcatcattcc gccgggcaac ggtaatttct
    10201 cgaaaatggt gcactcatag cccattaggg caaggtaatg ggcagccgat aggccggcag
    10261 gtccggctcc aataatggct atccgcttcc cattcttgct gatgttctcg gtttccagaa
    10321 tttgtttgta attctccgcg ggttgctggt cggcaatgta gcgtttaagc cagcgtatgg
    10381 agagtgggtc gccctgatgc cccacggagc aaacagtttc gcacttgtgt gtacaaatgc
    10441 ggccgcaaac ctctggcagc gggttggtgt cgtagaggat gcgtaaccct tcgttcaggt
    10501 cgtcgttacg tattgcttta atgtattcag gaattcccat gtgtgctggg caggttgcaa
    10561 cgcaaattcc acattctacg cagcggtcag cttcctttat ggcctgttcc ctggaatatc
    10621 cgcgaacaat ttcaatgaac gacttgtcgc gttcctcggg tttaagttcg ggcatatgaa
    10681 cgcgctcctt gctgtagagc tcgtagtttg gcccaggttt tttgtagcca ttgggttcgt
    10741 cgtcccattc ttttatgtcg accccgggaa taaaccggaa tacatctgga tcggaatcga
    10801 cccacttgaa gtgattcgac atggacagtg agcctgtggt gcaaacatca acgcaaaggg
    10861 cgcaccagca acagcggcca tagtcgatac ggggtcttaa cccgctatcg ccattttttg
    10921 tctcaatgcc ctcaaccgga accatatcga ttgccgcatt ctggcatatt gactcgcatg
    10981 ttccgcaccc tatgcaggta tcaacatcgt tcttgtggaa acccctgtat ctgggtgcag
    11041 cctctctctt gaggggttcc ttaatggtta caggctcttt aaccacattt ttccatgcgg
    11101 taaaggggct tatgatatct tttattcgtg ccatacttta cttacgtttc aataaattac
    11161 ctttcaattt caggtggata ggtgtgcaac gaggtcataa ggccagccac atcggcaagg
    11221 ttggtattga ttatcatttt ttcaagcaat gcaacggcat gggtgtagct tggcccgcga
    11281 acattaatac ggcgggggta ctcgctgcca tcggaaacaa tgtagtaggc aaactcgccg
    11341 cgtgtacatt cggaacggat aaaggtttca cctgctggta tttgccagtg aagcacattg
    11401 ggagtggatg ccttaaaatc gccatctttt ggcattttgg ccaggatttg gcggatgagg
    11461 tcaaccgaaa gaagcaggtc gcgacggcgg acatcggctc gggtatagat atcggaatcc
    11521 ttccccacaa cgggttcaaa gtcgagctcg gggtatttca ggtaagggta ctttttacga
    11581 acatcatcgg ggatgccggc cgcacgcgcg ttagggccgg taatgccaaa ctggtaaatc
    11641 cattttgggt cgatatatcc cataccaaca gtgcgtttct tgaaaacagc attgttgaac
    11701 atcaccctgt caatatcaac cataaactcc tcaattgttt tgagggtttc ctccatgcgt
    11761 gcttcaaatc catcgggcaa aagtcctcga actcctccgg gtatgatata catgtggtat
    11821 atacgtccac cggtaagttc ctcaaagcgg tcgagaacca ggtcgcggca gtagttgctc
    11881 cattgtccaa ttattcccat tcccagcgag ccggcttgtc cgcctatcca catcaggtag
    11941 ctttgcaggc gtgccatttc caatgtaaga gttctaagcc attgggctgt ttcaggaatc
    12001 tcaatcccag ccaactcctc aaccccagca gcaaaacagt actcgttgaa gtcgggttca
    12061 ggaacggtaa ttcggcaaac aatggggaag cactgaatcc atttgcggcg ctccatcagc
    12121 ttctcaaaac cgcggtgtag gtatcctacg tgggtttcgc aatccataat ctcgtcgccc
    12181 agaagggtta actcaagcga catgttccct gttatgcccg ggtgttgtgg gccatgccat
    12241 agcttcacat acttatgtga tctaaggtca atatctacct tgccctcgct gttaagcggt
    12301 gggtaaaggc ttctatcctc gtttatcttg aacagtttgc taaatttcat cggggtaaag
    12361 ttttttcttc atatactcct ttggatcatg agtttccctt cccggacgag ggaagtaggt
    12421 ttcctcggcg tacttcttgg tatcaaagtc gcggcggtag ggtgggatgt tgtcccaacc
    12481 ctccagtata aagggcttat caacccttgg gctaccggga aagtcaatgc caaacatttc
    12541 gcggagctca cgctggtagg ttgcagcttg ttcccaaagg tgatgaatgg tttccatcgt
    12601 gggattatcc ctaggaataa aagttttaac cattatatca atccttttgt ttggattgtt
    12661 caggatgtag gtgagctgga attgattttt ttcaatccaa tccacggcag taagctgaac
    12721 cagatgaagg tagccttcaa cctccttgag gtgagtaacc agtgttacca gttgttcctt
    12781 tggaatgctc atgtggtaga gccctttacg ctgtgtccac acttcggcat tagggaaaac
    12841 ggtttttatg ttatcaagta gtgtgttcat ggtcgtaact tttaccagtt ataatcgggt
    12901 atatcccaat ccttaattat ctttttctga tttgccttgt accaatcaaa gtttttggca
    12961 taaaggtttg ccccttcacc cttgccctgg cgaataagct ctttcagttt ctcaaaccca
    13021 gcaagaatgg cttcggggcg tggcatgcag cctgcaatgt aaacatcaac cggtaagtaa
    13081 tggtcgagtc ttttgatggt attgtagcta tcccaataca ttccgccgtt tattgtgcaa
    13141 ctacctagag caataacaaa ctttggattt tgcatctgct cgtagctgcg gataaccctt
    13201 ttcagggttt taacggaaag atatcctgat ataatgaata ccgacgattg acggggtgtg
    13261 gggcgccact gtatgccaaa acggtacgca tcgaaccgtg gggtcatgag cggacgcatc
    13321 tcaatggcac cgcaaccggt gccaaagcct aacacccata acgattccga gcgggcccag
    13381 ttcaaaaatt tttcaaatat gggaagtacc ggttcgtgaa cttttggacg agcgtcgcaa
    13441 aagtagtcct gaagcggatt tatctctatt ggttccccat cgggcgacat tactgtcctt
    13501 ttctctaaat cacttttact catggttata gtgatattac ttatttcata atcagattaa
    13561 aagaatggct ataattccaa aaagtagtgg aacgcctaag aaccagcgaa cgctttggtc
    13621 aaccctgaag cggggaaagg caaccccaac aaaaattgac cataggtata tgaaaaaggt
    13681 tttaatgaat agctcgggcc aactggttgc acctccatac agtaagttca tgaataggat
    13741 tagttttgcc acagggaaaa tagctcggtt aagctgcagc acacctagaa atccagaatg
    13801 gtactctgtg ggtgggccaa taggaatttc ctgtggcgct aaaacaacat caaagggagc
    13861 acgtcccatg gaacctaaaa aggtgagcat tagagcggca ccggcaaagg gattagtaaa
    13921 aatggtccag ttcaatatac cgccttgttg cgctgcaaca atatcgctaa tattgagggt
    13981 atggtactgc atggctattg atattactcc cagggtaaga ggtagctcaa gtgcagtaac
    14041 ctgcgaaaga cctctggaaa ccccaattgc cgaatatgga tgtccacctt cacctgcccc
    14101 taaggccata cccaatacac caaagaattg aaagaaaaga acaagggtaa ggtccccagc
    14161 aaacgataga ttatcaaaca taggggaacc atatattaca ggaaggaaaa aaagaagtcc
    14221 aattcctccg gtaagtctaa aaactgggcc taagtagtac attacaccat gtgtaatttt
    14281 gctccttttg gcatgattct ttattaagtc gaggtatggt tgataccatc ttataccata
    14341 acgtctccca actcttgctg ctattttggg cataatgccc tgaagaatta gcccgtaatt
    14401 taaaactata aaaagggtta gcagcaagta tagtatttgt ttaattccaa attccattta
    14461 gaatcctccg ttaattaaga tgtaaactat tacagtaaac cctaggatgt gaactaggta
    14521 agtttgtcca ttaccattgt agatgctcct gattttatcg gctatagcat gtgcgccttc
    14581 gccaacagca tcccaaaagt tggttatacc cggagctact aggaatccaa cggccttttt
    14641 atagggtgcg taaaagttat atgcaaaatg ggtggtttca gggcggaatg gacgttcagc
    14701 agaatataca atattgaact gctttacctt ttgggctttc cggtttatga tgtacagcca
    14761 tgcaaagacg gcaccaaaga taagcaccac tatggctagt ataagtttac cattccaata
    14821 cccaaatggt ccaatagcta attgcccatg ccaaaccaat gcaccttcag ggaagtagtt
    14881 taacagaatt tgcccaattg gttttagcat tgagtttggt agcgctgaaa gaaccaatag
    14941 tactgtaatt attatatatt gaggaacaag catccaaaaa ggagcttcct ttacgtttcg
    15001 gagttcatcc ttgggttgcc ccaaaaagat agtgtgaata agcctaaaac agtacaggaa
    15061 cgagattacc cctgcaaaaa aagcaattcc ggcctgaaag taccatccgt tttgcatgat
    15121 ggcgttgaac attaaccatt taccgccaaa acctgttaag ggtggcaatc ccgcaattac
    15181 tatgatacca atcagtaccg atacaaacga aaaaggcatt tgcttgatca aaccacccat
    15241 ttgatacata tacttggttt tggttcgcat tacaacacct ccaattgcta agaatagtaa
    15301 ggatttgaac atgaagtgtg tgaccacaat tgaaagggat gctaaccaac caaggtgcga
    15361 catcatggtt aaaccaaaaa gtgcatatcc cagtacacct atgcttgaat atgctaatag
    15421 ccttttagcg tcttcctgga atgccgccat catattaccc ataagagcgg taagaacacc
    15481 tacccagctt agcgcataaa ggacatcgat gccaaatagt ttttgtggcc ccatgcttag
    15541 gaatagcatt accaatccta acactccggc tttcaggaga attgccgaaa ccattggtga
    15601 tacatcggcc tcggcctcgg cgtgcgcgcc tggtaaccaa atatgaaggc ctattgacgc
    15661 aagtttggtc atgaaaccta ttgccattag ggtaaatgca ataggagcat agtaatgtat
    15721 gtcctttaat atttcaagcc ccataccagt atggcctagc tgtgcaatgc taaaaccagc
    15781 caggattaag taggctcctc caaccgagaa aagaatgtaa cttaatgcat gctccatcga
    15841 ttttttgccc ctgataatca ggaagtacga cccagctgtc attaactccc aggcaaagaa
    15901 gaattccagt gtggttttag cctcgagaag acctattaat cctgcaaaca tcatcattac
    15961 aagcgggtaa aatccagtac gtttaccctt aaaagcatag ccggccagga gagtaattat
    16021 accgccaatt acaaatatta taaagaagat actggttaac ccatggaatg tatcttgatg
    16081 tgaaaagtag tacaacacca ttgctccaat ggctaaggtg tttttgatgt aaacaggtaa
    16141 aaagtcaact atataaagga acgcaatgaa tgcggtagcc atatatataa aggtagattt
    16201 gtaacctgta atctctccaa aataaaatcc taaagcgtat gtcaatccag caaataatgc
    16261 agtaggtata aactgatgta gtttgatttt agggtaattc tgttctacct cgtcccattt
    16321 gattgcataa cccaaccacc ggaacaggta aaccccttca aagagcgacc caagcagaat
    16381 tattgctagt aaccagaaat ggtggttact tgccagttgc attaccagtt gccatttccc
    16441 aaaaaacgat gggaatggag ggaatccaac cagtgtaaaa ataaaagttc ccatcaggaa
    16501 aaggtagatt cgatggcctc taattgcacc ccaatccttt aacttatcca catttacaat
    16561 gcctgcaagc caaaacaaac cagctttagc caggtaatgc gagaggagga tggccaatgc
    16621 aatgaacttt gtattctcac caaggtaagg ctttaaccct attatggtca taattaaacc
    16681 aatttgccct accgatgagt agcccagaag ccgcctggtg ttttcttgtt taatcccaag
    16741 caggtttgat gccataaagg ttatggctcc gattatgctt agggcattga aaagggtaaa
    16801 atttccaaac accatcagct tgtccagaac aaaaattcct gcagtggccg atgccgctga
    16861 tattaaagct ccaattccgg gatgtgctcc ttcatacaca tctagcgccc atccattggc
    16921 tgggaagggt tttagctcca aaacaatgga tagtaggact agaaaaattg ctacatatcc
    16981 acctttaacg tataaaagcg acgcctggct tataaagtcg atattcagtg tgccaccaaa
    17041 gtagtaaatg aaaactgttc ctagtagtag cagtcctgag attaagccgg tggcaataac
    17101 atacttaaaa cctgccggaa tcgatttacg gtcagggtta agtattacca aacccgcaat
    17161 ggcaatgctc tgtatctcca taaaaacaaa taggttgaag aggtcgcgag tcattaccat
    17221 tacatttagg cctaatacta gtactagaaa cacaatcatc atgtttttgc cagcctgcaa
    17281 tagctcttta gccaggtaga ttgcgccaag taatccaact gtattaataa gaagcgtaaa
    17341 tatcgattcg gctaagccca tttgcaggtt aatggcaaag ggtggtttaa atcccccggt
    17401 aaaaatctga acagattctg ttgcattgcc ccagaaatgg cttaaccaac ctgccgatat
    17461 ggcaatcatg gctgccaggg cggtgtaaac aaccccaagg gctaacttct cccttttacc
    17521 taaaaatcca agcgcaaaag ctactcccag cgctatggaa actatatgta ttgggtttac
    17581 catttcaaat cggtgtatga gttaatatca agtgattttt tctccctata cattcgcaaa
    17641 acatatgcaa gcattagggc tgtaacgccc agtccaatta caattgatgt gagaacaagc
    17701 gcttgtggca cagggtctgc tactttgctt gcagcttgcg acacatctac agccttatcg
    17761 ataattggtg cggtgctgtt tttaatataa gccatgctaa caatcactat gttaaggcca
    17821 gtatcgaaca ggctgaaacc aatcacaatc tttatcaggt ttttctgggt aatcataccc
    17881 cataagccaa ctactatcag cataaagcct ataagcatgg caagaaactc aagtgtaagg
    17941 tattgcgcca tagctaaatc tcgttttggt tttccttaag tgaatcgagt atgcctgtta
    18001 gctcagagcc aaccttaatg ccaatgaaaa catatattat tggaatggcg cctgcgctta
    18061 aaaggcttcc gagtttgccc agtggtaaaa aagcgttgtt taggaaaccg ctagccagaa
    18121 taaggcccag tattcccatt gcaacaaatc caacccctga taccgattca agaaccgata
    18181 tcagcttgtg gttaaacctt gaggtgggtt gggcaagtat tatcatcata acacctgtgg
    18241 caataacagc gccaccttga aaacctccgc caggggtaag gtgcccatta acaaaaatgt
    18301 aaacacccag catgaaaatg gttggcacaa gcagggtgga cgctgtttcc agaatctcgc
    18361 ttgttttcct tagcttgttg ggcctaccat tatcggcatt cattactttt agaaagaagc
    18421 ccacaattgc cgcagcaatg aacaaaatgg tgacctcgcc tagggtgtcg aatccacgat
    18481 aggtaacaac cacggcagtt accaggttgg ctgcacccac ttcggcaggc ccgttttctg
    18541 cgtagtaacg cgccagcgga ttaagctcat tgctcccggt aaagttgatg agtaaactgc
    18601 cgattatagc aacaaaccca accagtagta gcagtagtat tcctttctta atcattggag
    18661 tgtcggattt tgtttaaaac gtaaaagaaa ataatcgtgg aaagtccact acctatcgat
    18721 gcttcggtta gtgctacatc gggtgctgca agtaaaaggt acagtaccga tgcaaaaagc
    18781 ccaactgccc ccgtggcaat aatggccacc tttgtgttct tgtggtagat ggcagttact
    18841 gccagcacaa ggataactac tcccagaaat attgcaagaa ttaaactcat agctattcct
    18901 cctcagtttt gttttcgctt tctaccaaga ggtcggcaac cgttttatcg gtcatttttg
    18961 tacccctaaa gtgtgctgcg cgcgaaatca cgttcgatga aaccgggttg gtgataagca
    19021 caaagaacat aatgactagc agtttccaaa tccaggcagg atttactatg gctagcccaa
    19081 ttaggcttag aatggttccc agggtcgacg ccttggtacc agcctgaatg cgattgaaca
    19141 ggtcaggcat gcgaactatg ccaaggccac ctaaaaatat gaagatggaa cccacaaggg
    19201 ttaccaatgc tcctataagt tcacgtattt ccatcacaac cccctttcga tatacctacc
    19261 tattactact acgctcagga agctgagtaa gccgtaaacc agagccacat ccagatagat
    19321 aattctaccg gcaaagtggg agattagagc aattaccgat attgaggcca cggtcatcac
    19381 atcaaaagca atggttcggt cggtgacatc gggacccttt atgaatcgga gcaatgccag
    19441 caggatgccc aaggccatga gcacacctgc cataattaga atggtgttaa ccataaatca
    19501 cctccagcaa cttttcgaat tttcccagaa ttttccggct tgcctcttcg gtatcaagcg
    19561 tttttacatc aatccagtgg atgtacagct catcatcctt catgtcaatg gtaagtgtac
    19621 ctggagtcag ggtgatagag tttgccagaa tcatccgccc tatgggcgat ttaagccgtg
    19681 ttttaacctt tactatccct gggtttatgg gaagtttagg gtttaacacc ctaagcgcca
    19741 catcgatgtt cgattttact atctcccagc taagcacaaa aaggtagatt aatgagtaaa
    19801 cgaacacctt aggggtcatg cgaaacctcc caaaaacgtt gctattcttc ccgaaaatta
    19861 gggcaagagt aacggcaagc gccaagccca atagtagact tgtaagctca aacgtaccgg
    19921 taagcagcaa ccaaatcagg aaaagtaaaa caaacaggtt tgctacattt ctgatattca
    19981 ttggtttgta gtttgttttg tgaattaaaa taaacagata tatagatatg taaaagtaga
    20041 catgttattt gactaacaaa taattaaagc agtaacatat tagggtaaaa agattaaatt
    20101 tgcaaaggtt ttcaattaac atatgttgat aaaaacgatg atttacatca ccgtttcttg
    20161 aattgtaatt taattaacag atggttgagt ctaaaaaatc atgcctgctt tgcaataaca
    20221 agtcggaatg ttttaggcag ctgagcgact ctcagcttga ttttgctaat agcaacaggg
    20281 ctgaaatatc gtaccgcaag ggggagatga ttgccaagca agggaacttt tatacccata
    20341 ttctttactt gcaaagcggg atggttaagg tttacaagga gttgcccgat ggcagtaacc
    20401 ttattttgag catatttggt gtgggcagct tgattggttt acccttcctt ttcaagaacc
    20461 agattctgga ctactccgta gctgcgcttg acgatacaac catatgcgcc atcgacaggc
    20521 atgcttttga aaagcttatt cacgagaatg gcgaatttgc cgccgaggtg gtaaaccagc
    20581 ttaatcagtg tacgctttac aactacgaca ggctggtaag cctcacccat aagcaaataa
    20641 atggccgttt tgccgatacg ctcctttatc tctccagagt ggtttacaag gctaatccct
    20701 ttaaccttac ccttagccgt aaggatttgg ccgaatatac tggggtttcg gttatgagcg
    20761 ttatacgcgc catcaaggaa tttaagaacg atggaattat tgatataagc ggtaataggg
    20821 ttgaaatcct taacttcagt cagctggagc ggataagtag aaccgggtaa ttcgtttagt
    20881 tttacaagcc attgattaaa attgagaata tgttagctga tcagaaggta gatctaaact
    20941 gcaaatggaa ttatttcaaa cctttactaa atccctttgc cgtatgctct ccatttctca
    21001 attagcgcag acatgtcgta tggtagctgc gagtcaaagt gcattttctt gttggtgagt
    21061 gggtgggtaa acccgagcgt tttggcgtgc agggcatggc gggggcatag ctcaaagcag
    21121 ttctccacaa actgcttgta ctttgtggtt aggttgccct ttaggatttt atcacctccg
    21181 taaacctcat cgctgaaaag tggatgcctg atgtactcca tgtgggcacg tatttggtgc
    21241 gtacggccag tttccagctt acattcaata agggttacaa acccaaatcg ctcaataacc
    21301 ttccagtgcg ttacagcatg cttcccctgc tcgccatcgg ggaatacctg catttttttt
    21361 cggtcacgga tactacgacc tatatgtccg gtaatcgttc cactatcggt ttccatatcg
    21421 ccccatacaa gcgcaatgta tagcctgtca gtgcttcgct caaagaactg ccgggccagg
    21481 tgattcatgg ccatctcggt tttggcaata accagcaacc ccgatgtgtt gcggtcgata
    21541 cggtgaacca gcccgggcct tacttcgccg ctcttgaaga gcggaacgtc gcgcaggtgg
    21601 taaagtaggg cattaactaa tgttccggtg aagttaccat gtgccggatg cacaaccatt
    21661 cctgcaggct tgttcaccac aagtaaagct tcgtcctcat aaactatgtc gatagggata
    21721 ttctcgggtt tgatatcggt ttcgcggggt gggtaagcca gaacaatgga aatggtatcg
    21781 agcggcttta ccttgtacga cgatttaacg gcctttccgt taaccagtat attgcccgca
    21841 tcggcagccc cctgaatacg gttgcggctc acattccgta tcttatcggt aaggaattta
    21901 tcgattcgcg ttggtgtttg tcccttatca acctcaatac ggaaatgttc gtacaggtcg
    21961 gcctgttccg ccgttccgtt ttccaaatca tcgtcggggt caagcagccg attttggttc
    22021 attgcttaat aatcactttg gaagtaaggg actgtccgtt ggcaaggttc acctttagta
    22081 ggtacattcc tgttgggatg tgttcaacat cgataggttc gttctgataa accgatttag
    22141 actcaattac ctgaccggtt atagaaatta gctggtatga atttacagca attccattac
    22201 caacatcgat ataaatcttt tcgcttgccg gattcggata aaccctgatg ttactggtag
    22261 tagatatagg tgcagtggtg ggattttggt aaagcttgcc aaaaataggt ctgatcatta
    22321 tagttccctc aaactgggtg tttgtccagt aaccgggtag ggtgtgaaaa atcctctccc
    22381 gactatttgt gttgcggtca aatccaacat tcaaaaactc aatggtactt tgctgccatc
    22441 ctatgtagaa gttgccaacg ggtatcgaaa cggggtcggt acggtaaacg gtaaagcagt
    22501 tcaggctatc ggtaaatagg gggcgggttg tgcttctctg gtagagtagt tcacccggtt
    22561 tgccattgtt atcggcccaa atggatattt taaagggctg gttattggca tcctggtagc
    22621 tgcggttaaa gtaaatcatt acgcctacca ggttgtcggc cttgtagctg taaaactttt
    22681 gggcaatcat ggcgttaatg gtaccctcgc caaaaaggcc atagccgctt tcggccgaac
    22741 catcgtcgag ggcgtagtag ttgtaaaagt tgagctggtg aaccagcgtg tcgttgtacc
    22801 ttaagtgctt ggttgattca tcaaaatcgg tttggagaaa agttttgagc atgaatttgg
    22861 cggagtcggg ccaagcagag gtgaaggtgt agtcgaatgg tcgggtgtat gtgaattcct
    22921 catacccata aatgttttcg gctcctccag aaaaggcgta ctcgttgccg gttgaaaggt
    22981 tggtaaccgc aaagcggcgg gtaacgttcc atgtggtggg accaaggttg ttgtaaacaa
    23041 tagaaaactc gcggggattc ttaatttcag aagcctcagc atcagcaaaa tgtgtccatg
    23101 gaaccgatgt gtagttcttc agcatcgatt ttattggttt gcgaaacgca atgtcgttgt
    23161 atatggtgtc gtcccagtta cgtagcctgt tcaggtaaac caaatcaata tgccaatggt
    23221 cggagttggt tttgtagccg ggcacagtgg cattctccat aagcgaagca tacccaataa
    23281 agcgaaaacg gaaaccgttt tctaagaaaa tgggattatc aatattgatg ttaactgtaa
    23341 agaatgttga gtcgagctta gtggaacttt tttcatcctt attgttgagc aggtggttat
    23401 agaaagcaat cttgttagcg ttgaagtcaa ccgatgaagc ccagcctcta acccagcggt
    23461 tttccgatgg gctatatagt tcaagcacca gcgagtcatt tgggctgggt tggtaaccca
    23521 accctccggg ttgaacgttg aacgacaggt aaatgctatc ggttgcaggg tagtttaggt
    23581 ttatggggag cgatgtaagc gtatcggcag gttgcggggt ggtggttagg tgctgatgta
    23641 aaactccctt gcggttgact gcatcgaatg ttactgcccc aacggtagga gggttaatgg
    23701 cataggtctt attggatatt gcattgctag gttcccagag ctctggggtg ggatagggag
    23761 tgtttcgggc aaaatcgtcg aaaaatggta aggacagatt cgtttttgtg taattacttt
    23821 tcttagtaac atcacttatg gtacgatagc ccgaaaggtt aaccagaacc tcctgcccgt
    23881 agcataggga tgatacaaat atgaatacta gaatggttaa tgctttccgc attgtattgc
    23941 tattcaataa tttcatcggc atgcttagcc ggcattgtgt taaccgagtc cgcacgctca
    24001 atctttggta tgcgtgcctg gtttagtgta agccatatat caaccctagt tccaaagcca
    24061 agggttgcgt tctccatgta ggcagggtac tggctataca ccctagcatc taacgagtcg
    24121 ttgtgcgtaa ttattgtttc gtcgtacctt attctaccaa cattgagcga tgcctcaatt
    24181 attctgctta ctgcatcgtt aagtttaaga cctatgagta gcggtattcc tgtacgttcg
    24241 ccctgcatac cctgacctaa aaccaggtcg atttttgagc ccttggggat gcgtgtgccg
    24301 ggttccgcaa ttttaccttc aaagcgttgt tcaagaacat tgtttagccc taggtcgggg
    24361 cggaagtata gggtgccaac ctcaaagcct tcctgctcaa ggatagcctt tgcttgtcta
    24421 agcgtgtagc caacaatatt ggggatttca attttgatag ggtttttggc attgatggta
    24481 acaaataccc ttcggttctt tttaaccatg gtaccaggtt cgggattctg ggaaattacg
    24541 cttcccggtt tcttgttaaa tatgtaaacc gaatcggcta tttcaatcct caggttgtta
    24601 tcggcaatca cctcagctgc ttcctctact gccataccca aaagtgttgg tgtaggttgt
    24661 tcctggttat gcctggtgta tatccttaga aagatcatga gtacaataag tactccaaga
    24721 atcaacccta atccatttat tatggtgtta acgtagggga ttttgtatat cttcagaagg
    24781 tactgtttta ctttctcgaa tgcagttttg gttgtcataa tgcaaggtaa ttaaacatga
    24841 tacaaaaata gcataaacat gtaataatgc taccaattag ctgtatacct taataatatt
    24901 atatagcgaa tcgcgctcgg ccggaatctt acccgcttct cttacaagtt gtgtcaaccg
    24961 gttaactgtc ataacaggct tttgctcttc ggctccggcc atggagtata tctttgtggt
    25021 atcgtcgata gttccgtcaa tatcatcggc gccaaaatgg attgcaaggc gggtgttctg
    25081 tatcccgagc attggccagt aggcctttag atgcttaata ttgtccataa agattcggga
    25141 tactgcaaaa ttgcgaaggt cctcggttag gttaacctcg cctaggtatc ccattgggtt
    25201 gtttgcggaa cggaacttaa gagggataaa gcagttaaac ccatgggttt cgtcctgaag
    25261 gttgcggatg gcctccatgt gttggaccct atgcttgtaa ctctcaatgt ggccgtaaag
    25321 catggtggca ttggattgta tgccattttg atgtgctgct ttgtggattg ccagccatac
    25381 ctcgcccgat gctttatcgg ggcatatttt ggctctcacg gccggatcga aaatctcagc
    25441 acccccgccg gggatggaat ctaaaccata ctctttgagt ttcctcaaac cctccgcgta
    25501 cgaaagtttg cttttacgaa tcacatagtc gagttcaatg gccgagaagg ctttaacgtg
    25561 aatgttcgga agtatctcct taacggtttt aatcattttc ccgtagtagt ccaaatccca
    25621 ttttgggtga acaccgccaa caatgtgcac ttctgtaaca tcgctatttt ggtagttgcg
    25681 ggcaatgttt ttgatttcat caagggtgta aacccagctc tccgggtcgc cagcaccttt
    25741 atggtacgaa cagaatttgc agttaaaaac acaaaggttg gttggctcta tgtggaagtt
    25801 cttgttaaaa agaacgtaat cgccatttat cctgctactt gtaatatttg ccaattgcga
    25861 tagtagggct aaatcggctt tctcgtaaag gtacacagcc tcgtttggct gaatacgttg
    25921 cccatccatt accttgcttg taatgtgttt gagaaattca tcgttgatgt tgggtgctat
    25981 tgtttttaca atgggttcca tgttaaaaat ttgcggtaaa tatacaccaa gaaaacaaaa
    26041 aaggcaggtt aattacccgc ctttatctgt tatgattgat tagttattat ttccggaaga
    26101 tttcatcgga aacagtcagt ctttttcttc cctttgcacg gcgggcagcc agaaccctgc
    26161 ggccattgtg ggtcgacata cgttcacgga atccgtgttt atttcttttt ttcctgatgg
    26221 atggctgaaa tgttcttttc atctctgtat gcttttacta attattattt ccagcaaatc
    26281 tatggctttt ttaaacccat tttgggttcg caaaggtaaa acaatttttc aatattaaaa
    26341 aacacctggt taaaaattca aaaaatagtt agccgcacat cccttaatca gtggtttggt
    26401 ttaggtactt gataaccgct gagttgacac ataggcgact actataatgc tcaatccttt
    26461 aatgtgcatt ttattgataa ccaatgtact aaaaattatc aaaacttata ttgcaccatt
    26521 taggccagat tttgaatcaa cccacttttt cctttatccc ttatccttta tcctttatcc
    26581 tttatccttt atcctttaac ctttacctca cacctcaata tgtatcacat acttttcaac
    26641 gaagtagtcg tcctcaaaaa aatcgtcaat attccatttt gtagcccact tgtggtaggg
    26701 ctttagctcc tcttttatgt cgcctccttt aagacagatt aggccgttag gaaggttgtt
    26761 aatacccccg gggtgaatgt ttttttcaac ccaagccata agctttggaa caggagccac
    26821 tgcacggctc acaacaaaat cccactcctg ttttagattc tcagcgcggg cggtgattac
    26881 ctccacatta tgtaagccaa cggctttggc tacctcggag gttacctttg tttttttagc
    26941 tatggaatca accagggtaa acttacagtt tgggaaaagg atggcaaggg ggatgccggg
    27001 aaaaccgcca ccagtgccaa tatccataat ggttgtgcct ttggcaaact gaacaacctt
    27061 tgctatggcc aaggaatgca gaacatggtg aatcatcagg ttgtcgatat ccttacggga
    27121 gataacgttg atttgtgagt tccatagcct gtaaagcggc tcaagttgtg caaactgatc
    27181 gcgttgagct tgagttagct caggaaagta ttgtgcaatt tgttccatgg caaaatgtat
    27241 tatttatggt gtggcggaag gttgctcagg atgtttgaga gcagctcacg ggctctgaaa
    27301 agttgagcct ttacggttcc taacggaagg tctagctctt cggcaatctc ttcgtaggag
    27361 tactccttaa agtagcgtag ctctatgagc ttgcggtagc ggggtttaag cttggaaacc
    27421 acttcctgta tcaggcgtat gttctgttcc ttaattagat tttcctcagg atcaggtgtc
    27481 gatgccggta taacggacga tggcgacatg gagttgcctt cggcatcctc tgggccttgg
    27541 tcaatcgata tcaggtttgc cttgcgcttg cgaatgaagt cgatacagtt gttcgatgca
    27601 attttgaata gccaggtgct aaatgcaaaa ttcggtgtat actggtgtat gttcttgaag
    27661 gctttgccaa aagcctcaag ggttaaatct tccgcatcgc ttttattgtt aaccattttc
    27721 agcagcatgt agtatatggc atcccggtag cgatccataa gctcagcgta agctttctgg
    27781 tcgcctttaa ttgctttatc cacaagcaca agatcgtatt tcgccttctc cgaaaggttt
    27841 tccgggatca ttaccatttc tgctgtcgtc ggttaagagt gttaaagatt agcaatttag
    27901 cataaaaata gagagaaaac aaatcccacc aaaatgcaat atgcattagc tttttttctt
    27961 caaacttttt tgctgcaatg gcagctatag tgtgttttac aataattacc agcaaaagta
    28021 aagcagttga tatcaaagga agaattttga aaattagggt taacgcaaat agaatgtaaa
    28081 caagtattct gcttaatggt tcaagcccta aacgggtttt caggttgctg ctgtagaatt
    28141 tggctgttga taggtgtctg cgcttttggc gtaaccaggt agaataagtt ttccgaggaa
    28201 ctgagagagt atgtgcctca gggcgatact caacagccgt attgcttggt gttgcaacct
    28261 ccatcacaaa aaggtcatcg tcgccggagt cgatacccac atggttggca aaccctttat
    28321 ttttgaaaaa tagcgatttt ttgtaggcca ggttacggcc tacgcccatg tatggtctac
    28381 gggctaacgc ccatccaaag tagtttaaag cgatgaaaag cgtatcgagc ctaactagtt
    28441 tattgagtaa acccctttcg gccaaatatc cgccatagcc tagcaccacc tctgtgccat
    28501 tggtgaagtt cgacgccatg tagcgtagcc acttgttcga ttttgggaag caatcggcat
    28561 cagtcagcac caaccattcg tttttggctg cctttatgcc aaccgtaagt gctaattttt
    28621 tggtatgggt aaatttttca tcttccttaa tggttgttac acgcagccga gggtatttct
    28681 tttgcaagct ctcaagtacc agatcggaat tatcgcttga acagtcgttc actaccacca
    28741 cctcgtagtc agggtagtcc tgctcaagga ctttgggaag gaaatgctta aggttttcct
    28801 cctcgttgcg ggcgcatatt actaccgata gaggtgggta ctgttcatcg ttttcgggta
    28861 aatcattaaa ttttttacgg ataaccctta ggtagaaaac caggtagaag taaagctgta
    28921 taagtacaaa tactaaaaag acaacaccaa ttactttttg gtaaatgctt aaattataga
    28981 taatttccat tataagacaa gtagtaattt tcatgcaatg atattaaatt ttggctgaat
    29041 ttcaagtgaa gtttaatcat gatttgtaac aaggggtttc aaatttgttt cttgtcagta
    29101 tttgtgagtt caacatagcc atgttcaatt actttactac atttgtgaaa aatttataaa
    29161 gatttgagtt ttagtagatt taatttaaac cctgcggttc ttgaaggtat cgaagccatg
    29221 gggtacacaa aacccactcc tattcaggag caagccatac ccgtaatttt ggaaggtcgc
    29281 gatttactag cctgtgcaca aacaggaacg gggaaaactg cagcatttat gctgcctatc
    29341 ctcagcctat tgtcagctga agaacatgat gcttcgtcgg tcaataccct tgtaattgtt
    29401 cctacacgtg agcttgccat gcaaatcgac caacaggttg aagggttttc gtactttgcc
    29461 ccggttagct caatagccgt ttacggcggg aacgatggct ctaactggga caggcaacga
    29521 caggctttgg agcatggcgc agagattatt gttgccacgc cgggccgatt aatgcagcat
    29581 attcaaatgg gctacgttaa ccttaaaggc gttcagcacc tcgtgctcga tgaggctgat
    29641 cgaatgctcg acatgggatt ttacgacgat atcatggcaa taattgccta tttgcccaag
    29701 caaaggcaaa ccctgatgtt ttcagcaacc atgcccccta atatacgtaa actggcaaaa
    29761 tcgatacttg ttgagccagt tgagataaac ctggctgttt ccaagccagc tgaaaatatt
    29821 ttacaggcgg tttaccatgt tggagcctcg caaaaactgt cgcttttggc ctcgcttttg
    29881 agcaagaagg aggtggtgca gagtgccatt gtttttgcgt caaccaaggc cgaggtgaaa
    29941 agcattgaaa gcaaactaca caaggaaggg ttaagcgttg ctgccatgca ctctgacctg
    30001 gagcagaaac agcgcgagga ggtgatgcgc aattttcgta accgtaaggt tcaggttctg
    30061 gtggctaccg acattgttgc acgcggtatc gatatagagg atatcgatct agtggtaaac
    30121 tacgatgttc cgtccgatcc tgaagattat gttcatcgta ttggtcgtac tgcccgtgcc
    30181 cagtccgatg gcgtagccat tacttttgtt agccccgacc aacgcgggaa gttgcatggc
    30241 atagagcgtt tcctgggtac aaaaatattt gtcatcaact atattcctaa tagctaagct
    30301 taggcaatga agaaatggtt actccgaata gtattcccaa tcctaccggt agtaatgcca
    30361 taattttcat ttggtcaggt tattaagggt cgagttgccg acgataccgg taggccaatc
    30421 ccttacgctt cgatatattc caaggaactt aggcagggga catcatctaa ctccgatgga
    30481 gactacatgc ttagggttcc cagtggtgag tattcctttg tttttcgggg tcttggttac
    30541 gaaactgttg agcggcggat taaagtagat gtaggtgagg taaccctaaa catcaccatg
    30601 cctgtaaagc catttctgat tgcccctgta accgttggag gcaagggcga agatattgca
    30661 tacgatgtgg ttcgcaaggc aattgccatg gcgccatact accgcaatct ggttagtgag
    30721 tacgaggcgg aggtttacct taaagggact ttcaaggtcg acaaactgtc gtggttggtt
    30781 aagcgaagca taaaggatag cccaaatccg cccaaggagg gttcgcttta cctgcaagag
    30841 tcctacaaca acgttaggtt tacagcgccc gaaaaatttg atcaaagggt aaagatgatt
    30901 cgttccaatt ttcctggcga gttaaacagc accgataata ttctgggttt tgtttcggca
    30961 aatctatacc aacctaaggt tggtgaaatc attttacccc tagcgcctta cgctttcaac
    31021 cattacctgt ttaagtacga gggttacgcc ttccaggata accgcgcatt ggttaaggtt
    31081 aaggttattc ccaagcgaaa aagtcggcaa ctggttgagg gctacatgta tattgccgag
    31141 gattactgga acctacatga aatcgatttt actgttcagt cgcttgcggg gccatttcgg
    31201 attaaacaaa cctttgggga ggttgagcgt aatgcttggc ttcccattag ccagtatttt
    31261 gatattgatg ctaatttcct tggcaacgtt ggccgaatac agtatgttac ggctatcaag
    31321 tatctgaatg taaaactaaa ttcaaaagta aaaccacctg ttaagattga tataaaagaa
    31381 gcggaaacca agcatattgc cgaggataca caggtcaaaa gaaatacttc gccccgaaag
    31441 caaagggaag atgcccgtat ggaacaactc ttggccaagg ataatcttac aaatcgtgaa
    31501 atgtatgagc tgcaaaagct catgcaaaaa caagtacgcc aatccgattc tcttcccaat
    31561 aagcttgagg tgcctaaccc tctaaccatt acagtcgact cggttgcaac caaacccgac
    31621 accatggcgt ggcagcaggt taggcctgtt gcgcttaatg ccgaagagtt caaagtgaat
    31681 agggagatag atagtaagct acagatgtcg cccgatagcg tttcagccga tagtaccggt
    31741 aaggcaaatg tacttaaaac cctttacatg gggcacacct ggtataacgc caataaaaaa
    31801 cgtacatttc tattctcggg ctttgctggt ccaaatgagt tccgcttcaa cactgttgat
    31861 ggttttgtag taggggcttc gggtagctac cgtaagcagt acagcaggaa tggaatttat
    31921 atcaaacaat cggcatacta tgcaactgcc cgcaaaacgc tcatgggtga aaccgatttt
    31981 cggctttcct atgccaatat gaataggggt ttagcatcca tccgaatggg ttggataagt
    32041 tacgatttca atggcgatgc gggtattagt ccattaacca atacctcggc ctcgctttgg
    32101 tttggccgaa actacatgaa actttacgaa aaccgatatg tgtcgctttc gaaccggatt
    32161 gacccattaa acggatttga ggtttacacc tcggtttcgt ttgcgcgcag ggttgagctg
    32221 caaaataata ccgattttat tgtttaccag cagaacctgc gatggtacac cccaaatgtt
    32281 cctgctaacg aggatgccac accccagaac ctatcaactc atagttcgct tgagggttcc
    32341 attcgggtgc gctacactcc gttttaccat taccgaattc gcaatggtcg taagcaaatg
    32401 ctttactccc gctggcctac cattaccttt caggttcata aggctttacc caatttactt
    32461 aattcaaatt ccgattatct cagatgggaa gccatggtta atcaaataat cagaaccggt
    32521 ccaagtaatc gaatttccta taaatttatc tacggtgatt ttgcaagcaa ggggagcctt
    32581 tactttgtgg attttaagca tttcaacacc atgcgggttt cggtggtaat tccctctttt
    32641 agcgagggtt accagaatat cagttactac cgcaggagca catcggagtg ctatgcgcaa
    32701 gcttttctga gttaccaatc gccgtacctt gctttaaagt atttgccatt cctgagcaat
    32761 agaatttggc tggaaaactt gcagttgact ttccttaaaa cagcaggcta taagccttac
    32821 tatgagattg gctacttttt aagccagata ggcgttgtgg ggggtattgg tgtttttgga
    32881 tcgtttgagg gtaagcaatt ccactcgcta tcggtaaagg tatcgctaat tattcctggc
    32941 tttgagtagt atgctaaact gtttgtttag tacttagggg gggctgcatt tgtttttttt
    33001 tacattgaaa tctaaagcat ataatctatt tttttaacgg tttagaattc agtgtaatat
    33061 cattattttt ttgtgttttt tgtaaaatga atgacaattc attaatattg ccacatgaga
    33121 aaaaaggacg aagccaaacc ggaagtaatc tttgaggcaa gccttgatgt acttataaat
    33181 catggttttg tgaacttttc aatgggaagg gtggccaaac aggcaaaaat agctgtgggt
    33241 accatctatc tttactttaa gcataaggat gatttactca atcaacttta ctttaaactt
    33301 tacaaagaga gtgtcgatag gtttttggag cactacaatc ccaataactc atttagagag
    33361 agtcttaaac tggtttgggt caattacctt aagcatcgca taaatcatta caaggagagc
    33421 cacttccttg aacagtacta taaatcgtca tatattaacg atagccataa acaaatggca
    33481 gagcagatga aaaagccaat acatgatatc attcaaaagg gtaaggaatt aggtctcgtg
    33541 aggagcgatt tggataatga aatgattttt ctggggatgc ttggatttct cagggagtta
    33601 gccgatgagc atgtaggtaa cgtttatatt cttgatgaag ataggattaa taaagccttt
    33661 gaaatgagct ggaatatgat taaagcatga ttgtaaaact taaattttga tgtttatggc
    33721 aataccattt tggcagtatt tggtttcgat gccactttac attgtactgt taatgctggc
    33781 agtggagttt atgcgaacga attacaggtt tgcagctgta ttctgggtat tatcgctttt
    33841 tacttttcct ctttggttta acaaccttga gggttggttc cgttgggcaa aaacactttc
    33901 ggttttattg cctacagcct ttgtggttgg ctgtgcccgc attgcccaat acgaaaaacg
    33961 cgatggatgg tggacaatct ttcgaaagga ttgggtgtta tggtttctgt atgctattct
    34021 tgggcttaac atactggaag cctctttgaa ggattttgaa atgggcaatt ggttcaatgg
    34081 cctatcggga ttaatactta ttgtaacgat accgcttgta aaatcaggaa agggtaaacg
    34141 gtatggatgg aagatttccg aggagaaacc tggcgactta attgcttaca ccgatgctat
    34201 atggaacttc ctatatacca gctggaatat tgcttttgtt tatgctgaaa atcccggata
    34261 cgcagcaagt tcgctttgca tcctattagc agctgaactt tatcctgtaa ttaaacgcag
    34321 acccgaatta tatgttcaag cccgcgtata taccttagcc attcacattc ttattagagc
    34381 tacatatgat atttttacgc caatcatgga ttcgtcggca tttgcaagcc aaaacgttgt
    34441 attttggtgg ggtatagtta acttctcgct tcatttgcca tatcttattt ggtatttctt
    34501 tttgaagaaa actgaaaagt gattgttctt taataatgta gcactaattg cgcgcatcga
    34561 ctgatgcgtg caattatttt gtatagtttt catattgata attttgaaac cagttaaccg
    34621 ttggcgggtt tactttacga agccgaatag attaatgttg aattgatttt atgaactaaa
    34681 ttgaaacgtg tattggtttt agcttgaaaa ttatttagat gttgtattag tgcacttgtt
    34741 ttttattgct ccgtttcaat cttcttcctt ttcgttattc gttttcccct tcaccacaac
    34801 tacaaattcc cctttgggag atttggagct gaaatgctcc ttggcttggg caagtgatcc
    34861 gcgaaagttc tcctcatgca atttggtaag ctcgcgcgca atgcacacct gcctatcggc
    34921 tccaaaaatg gggagcatct gctcaagcag tttaacaata cggaatggcg attcgtaaag
    34981 tattatggtt cgttcctcgt cctttagtgc ttcaagcctt ttattgcgtc cttttttttg
    35041 ggggagaaat ccttcaaata tgaatctgtc acaggggaat ccactctgca caagtgcagg
    35101 aataagggct gttgctccgg gtaggcactc cacctcaata ccccgctcaa cacaagtgcg
    35161 aaccagcaga aaaccgggat ccgatatgcc gggcgtgcct gcatcggata ttagggctac
    35221 cgattcacct gcctctattc ggcttgcaag gtaatcaacc gtttggtgtt cgttgaattt
    35281 atggtgcgat ttaagtggag tagcaattcc gtaatgcttg agcaggttgc tactggtgcg
    35341 tgtatcctcg gcaagaataa ggtttacggt ttttagcaca tcgagcgctc gcaaggtaat
    35401 gtcggcaagg ttgcctattg gagtgggaac tataaagagt tttgacatga agcaaagtta
    35461 ttacgatagt tttcttcgaa ccaagtcaac aaataaagtg gtaacctcat tggttgggtc
    35521 ccagctaaaa ttctcctgga ggtaaataat ggcctcattt aaatcagtaa gctggttgag
    35581 gtgtttaatg gtttggttgt aaaggtcgga atcgccgcca aaaagttcct tgatgaatag
    35641 gaacttatcg ttaatgccaa ttgccttggc aaggtcggtt atgggtttat tctgcagttt
    35701 gctctgcatg tcaacctttt ggtgttgctc ggctattacc tcgttgcgga acttgcgctt
    35761 accctggtac ttatcggcaa gtatggctga actttcctcg tgtgattgct gtgtggaagc
    35821 cggagtgggt tttggggatg gacttatgtc agcctttaca gggtttggtt cctgcttagc
    35881 tgtcggaatg ctggaaggtt ctgcgctctc ctcaactact gttgtttttt tagtttcagg
    35941 tatgcttgct atagttttct catgtggttc aaccattttt ccggacggaa ctacttgtgg
    36001 tttctcatgg gttctggaag caacttgttg cctatcaagc agaaggtctt catatattga
    36061 ggccagcttg gcaagtagta tatcgcgttc aatggaaggt agttttccgg ctgtaagtgc
    36121 cttatcgatc atggcaaccg cttcaactag ttgattccgt gcattcttta ggtattgcat
    36181 tgcaaattgt tttttgttcg catttcaaaa ataagtttta ttacatttgt ctgtaaaact
    36241 aactcaaaat atttatttct gaaccaatgc cttttcttga aaaccccgtt agtgggaaac
    36301 gtaagggata tatcgaggtt attgccggct caatgttttc gggcaaaacc gaagaactga
    36361 ttcgtaggct taagcgagcc aagtatgcca agcagagggt cgagattttc aagcctatga
    36421 ttgacacccg atactctgag caggaggttg tttcgcacga tgccaattca atactttcta
    36481 ccccggtttc atcgtcgggg aacattctta ttctggcaca gaatgtcgat gtggtgggaa
    36541 tagatgaggc gcagtttttt gatatgaatt tacccaatgt ttgtagccag cttgccgata
    36601 tggggataag ggtgattgtg gctgggctcg acatggattt tcgtggtaaa ccttttggcc
    36661 caatgccaaa ccttttggcc attgcagagt acgttactaa agtacatgcc atttgtgtta
    36721 agtgtggcga ccttgcccaa tactcacatc gtaaaaccga tgccgacaag ttggttctac
    36781 tgggcgagac cgacatctat gagccgctat gcaggctatg ctacaacgag gtaacccgta
    36841 agaatagaaa gtaggctttt tcgacccgat gtataacatc agcatcatgc aaaaaattaa
    36901 caggttgggg aaattgctag ggccagttgg atccattgct gggtatttta ttgctttggt
    36961 gggaattatt caacttttta gctccttcct agcaatagtt ttgattatca ttggactatt
    37021 cattggtttt acctcttttg ctgcccaggt tgattttgga aataaaaggt ttcggaacgc
    37081 attgttgctt tttggaataa ttcctgttgg gaaatggcaa agcattgata gctcaatggg
    37141 tttgagtgtt aaaaggttga atagggcatg gaggacatac agctcaagta ataggtcgtt
    37201 cgatattgct gatagcgatt accgtattgt gattgttgat aaacaagtga accctcttgc
    37261 tgatatccaa cgagttcaaa gccttgaaaa ggcaaacgag acactggttt attactccga
    37321 aaagttaggt ttgcctatcg ttaagcaagg aagttagagg attttttatc atctgcatcg
    37381 atgaaaaccc aggttgacat atcgcatcga atatcgccaa aacttttttg gtttgttact
    37441 ttgatgataa taactctgcc tataaccaca caataccgtt tgttgatttt tggggagtac
    37501 actaccggtg aggttgttgg agtggagtat gaaaaaacac taggaatatc ggcttttggc
    37561 cccgataagt atcctataat agtttttact acgcttgaag gcaaatcagt tcagttttac
    37621 ggccttgaaa attttgagta tcccaaaggt actatattaa aggtggttta cagtcccaat
    37681 aaccccaccg attgcagggt ttttagcttc ggggctttat atactgaccg aaaagccata
    37741 atcccgggaa tacttttttt actttggatg gctttctacc ttgcttttag aaacaaaaaa
    37801 tagattttgt aaatttgttg aattatattg caccaatcaa ttttaaacat tgatgattaa
    37861 cctaactgct atacagcttg ctgaaattgt tctgggtcaa gtaattggcg attcgggttt
    37921 tactatcaga aacgttatta ccgatagtag agtgcctgta tcgggaaacg gctccatgtt
    37981 tattgctcta gttggcccaa atcacaacgg acacgagttt atcggtgaac tttataatat
    38041 tcgaaacata aaggcatacc ttgttgaaaa gggtgaggtt gactacacaa attatccgaa
    38101 tgctacattt gttgaggttg acgataccct ccaggcccta caaaaacttg ctgcctacca
    38161 ccgcaaccga ttcgtttacc cagtaattgg tataaccgga agcaacggaa agaccatagt
    38221 taaggagtgg ctcaaccagc ttcttttctc ggaataccaa attgtgcgaa gcccgcggag
    38281 ctataactca caggttggtg ttcccctgtc ggttctccaa atggataacc atttcgattt
    38341 agcaattttt gaggcgggta tatccaaacc gggtgagatg gttaaacttc agcccataat
    38401 tgaccccact attggtattt tcaccaacat cggcatgccc catcaggaga atttcgatga
    38461 tattgatgaa aaggttaacg agaagctgaa gctgttccgc gaagttaaaa cgcttattta
    38521 ctgcaaggat cacgcggtaa ttgatgctgt tgttcaggat aacactttgc ttgccgataa
    38581 ccaactgctt acctggggac aaaataagga gtcgaaggtt caaataatga gcgtaaagcg
    38641 cgaagctggg aagactctgg ttcatgttcg ctttaacaat cacgagcaca caataacgat
    38701 tccttttacc gatgctgcct cgtttgagaa tgccatgcac tgcgtggcta ccatgctgct
    38761 attaggttat agcttacagg atatcgatac gcgcttacag cgcctgcaac ccgttgcaat
    38821 gaggcttgaa ttaaaggaag gtataaacga ttgttcagtc attaacgact cctacaattc
    38881 cgatttgggg tcgttagcca ttgccattga ctttttgatg cagcaaaagc agcacgaacg
    38941 gcatgtagta atactttccg atattctgga aagtggctat gcacctgaac agctttactc
    39001 aaaggttgcc caaatgctcg aggataaagg ggttgatcta ctcataggca ttgggcctga
    39061 aattagccgg tacgactatg cctttgagat tgaggccgag ttttacagca gcacacatga
    39121 gtttttgctc gatctaaagc gtaaggagat tcgcgatgcc gctatactgg ttaagggtag
    39181 ccgaaagttc cactttgagc agattagtgc tgcgcttgaa cgaaaggttc accgaaccac
    39241 actcgaaatt aacctgaatg caattgccca taacctgaac tactaccgca gcttgctgaa
    39301 ccccggtgtt aaagtgatgg ctatggtgaa agctttttcg tacggcagtg gctcctacga
    39361 aatagccaac ctactgcaat tccaacgtgt cgactatctt ggtgtggcct acaccgacga
    39421 gggtgtatcg ctcagggagg caggtatttc gttgcctatt gtggtgctta acccatcgtt
    39481 tgggacttat gagctgatgg ttgagtttaa ccttgagcct gaaatctata acattacagg
    39541 cttaaaggag tttatcaacg ttttgaacag ggttggggta agctcgtacg gtattcatat
    39601 taagattgat agcggaatgc ataggctggg ttttactccc gaccagttgg atgagcttat
    39661 agccatacta aaatcaacca attcagttag ggtaaaaagc atattttccc atttggcagc
    39721 cagcgatgag ccacatcacg atcaatttac aattcaccaa attgaaacct tccacaaggc
    39781 ttgtaacgtg ttaagtgatg tgttagggta tcaacccata cgacatattt tgaacactgg
    39841 cggtatagag cggtatcccg aggctcagtt cgaaatggtt cgcttaggga ttggcctata
    39901 cggcataagt cctacgcatg gcgataagct aatgcccatt agtacgctaa ggagcaaggt
    39961 gattcaggtg aagcaaatca aacccggcga cacagtagga tatagccgaa agggaatagt
    40021 tgatacgcct acaacggttg taaccattcc ggttggttat gccgatggct tatcgcgtat
    40081 gctgggtaat ggcaattaca gcatgatgct gaacggtacc ttggtgccaa ccatcggcaa
    40141 tattagcatg gatacgtgta ccctcaatgc aaacggggtt gaggttaagg agggcgacga
    40201 ggtaattgtt tttggaaata aacctaccat aaccgacatg gcaaaggcca tggaaactat
    40261 tccgtacgag gttcttacct caatttcgca gcgcgtgaaa cgggtttact ttcaggagta
    40321 acctatccca aatcgctaat gcgttccaac ctatgatggt caaggatttt tatcctgcga
    40381 ccgtcaagtt caattacacc ttcctcggca aagcccgaaa gggttctgat tgcatttgag
    40441 gtagtcatgt tggagaggtt tgccaaatcc tcgcgcgata ggtaaacctt gatggtttta
    40501 ccgtcgtcct caagcccgta ggtatcgcgc aggaatatga gcgattcagc caatcgtcca
    40561 cggatatgct tttgggttag ggttacggtg cggttggtgg agaatcccag ctcggtggca
    40621 accgatttga gaataattag cgtaagttct gcgttgttcc ggagaatccc cataaaggca
    40681 ttcttttcaa tgatgcacac cacggaatcc tcaagagctt cggctgtagc ggagtagttc
    40741 tcctcagcaa ataatgccct gtatcctata aaaccgatag gcttggccat gcgaacaatc
    40801 tggtcgcgac cgccaactcc ctccttgtaa agctttactt tcccctttga gaggcatacc
    40861 aaaccctgtg gggtatcgcc ttccttgtat attatctcac ccttgcggaa atactgacag
    40921 gtgatattat tgcgcattat ctccctttcc tctggtttta ggttagggaa aagtccgcac
    40981 accccttcaa atacgctttc aatatcgatg ttgtaggaat ttctggacat ttttttttca
    41041 aaaatacgtc attttatgct ttcaaacata aaattaacat aattttagca tggatggttg
    41101 tgttttttaa tatttacggg ctatttagct ccatttagta accaagggca tttttcgtcc
    41161 atagccaaat gcctttgatg aaaccctaag tattggtggc gactggtagc gcttatactc
    41221 attcatgtta acaagcctta gggtacggtt taccgtatcg ctgggaaagc ctaaacctac
    41281 aatttcttcg gcggagaggc cttgctcaat gtatgcaaaa agaatggggt caagtatttc
    41341 gtagggtggg agcgagtcgg aatccttctg gttgggtcgg agctctgccg atggcggttt
    41401 ttgaatggtg tttataggga ttatttcatc gttgcggtta atgtagttgg ccagcctgta
    41461 aacatcggtt ttgtaaacat cgcctaaaac gctaagcgca ccgcacatat cgccgtaaag
    41521 cgtgccgtaa cccacggcag cctcgctctt attgctggtg ttcagcagta aatgaccaaa
    41581 cttgttggag atggccatta acagggttcc ccgaaccctt gcctgtatat tctcctcggt
    41641 gatatcctcg ggtcggccct caaaaataga gcttagctgg tttttaaacg actcaaaaat
    41701 gggctgtatg gaaactatgt cgtattgcac gccaagtttt tgcgcaagct ctacagcatc
    41761 gtcaatggag tgctgcgatg agtactgcga tggcatcagc agaacacgga tgttgttaac
    41821 atcaagagcc tgtgcagcaa gggctaacac aactgccgag tcgataccgc ccgaaagccc
    41881 aagggtggcc tttgtaaacc ctaacttacc gaagtaatcc ctaatgccca taaccaaggc
    41941 attgtgaata cgctcaatcc gttggtctga agttgccgat atttcagggg gtatggattg
    42001 gttgcgctcc aatacgttag tgtcaatcac cctaaaatct tcctcaaagc ttttcagctt
    42061 ttcaatgata tcgcccccgg cgttcactac catggagcca ccatcgaaaa gcagatcggt
    42121 attacctcca acctggttta catatattac gggcttggaa tatcttgcca cattgctttg
    42181 gaaaacctgt aacctgcgct cctcaacgtt gtagctgaac ggcgaagccg atatgtttat
    42241 caccatatcg gggttcagct ttaccaggtg attcatgggt gcatcgcggt aaagcttact
    42301 tctggaaaaa gctgtagtgg tgggctggtc atcccaaata tcctcgcata tggttatggc
    42361 tatttttatc ccatcaattt cggcaacacg gtagttgccg ggattgggct caaagtagcg
    42421 atactcatca aaaatatcgt aatcaggaag tagggttttg tggtaagcat ccagcacttt
    42481 tccctcgccc aaaaggtatg cggagttaaa aagcagcttt cccgtagtat cagtattgat
    42541 gctgggggcc ccaactatag cataaattcc attgcagtac tgtgcaattt ggtttacggt
    42601 ggctatgctc ttatccacaa aatgcttgtg ggtgagcaaa tcgtggggtg gatatccaca
    42661 caccgatagc tccgagaata caactaacct tgcaccttcg gctttagccc ggtttatcga
    42721 tgcaattatt ttatcggcgt tactgtcaaa gtgaccaatg gtgaagttca gctgtgcaag
    42781 cgctactttc attttgtgtt atccaaatag ttagaaaatt ataccaacct tcaacgataa
    42841 actttgcgat gtaatggttg agtttggcag ttcgatgatt ctggttaaac catcgcacca
    42901 ggttatgcca aacagaatgg cgctctcgcc gccgagtgag tattcacctc caattcctat
    42961 ttggtatgag gcaaaccccc atgccatatc ggttttggtg gtttcgcggt caacatggtt
    43021 ggtttcgttc catgcgtagc ttcttattct gaggctcccg ctaacgccaa cattggcaaa
    43081 gtatgtggta tagccaatct ggtttgtccg aaacttaaag ccaatgggca gcgtaaggta
    43141 ttgcgatttg aatttgatat tgtcgccaat ggcgatgttg taggtgccat cgatagtttt
    43201 cagggtatag gtggattgca taagcctgat gttcccgcca agggtgttaa ccgagagccc
    43261 tgaggaaaag gcgtaccgtt tggcaaagaa tttatcggct gtaaagccaa tgtttaagcc
    43321 cacaacaggg ccgttgggat cgtaacgttt ggtatcggag ttgaaccacg atagctgcgg
    43381 gtcagcaaat actccaaagc tgtattgctg ggcatttgcc agttgattac caagcagaat
    43441 taaaccgatt aagagtgtaa ggctattcaa cttcattgtt tcatcatttt ttgtcttacc
    43501 ttgcaaaggt aaccattatt ttaaagcgac ttttcggatt gctgttccaa aaagtaaaac
    43561 catatttcac tttagctcta aatgtaacgt tgattacatt gcctaagtat taactttgtg
    43621 acctaaatta tgctaaaaca atgattctgt ttccaaatgc caaggttaat ctgggtttaa
    43681 acatagtgcg taagcgcccc gatggctatc acgatattga aactctgttt taccctgtaa
    43741 agggattatg cgatattctt gaggtagtgg caaccgatgg cgaggggaac accattgaat
    43801 tcacccaaac cggaattggc attgattgcg ataccgagaa caacctgtgt gttaaggctt
    43861 accgccttct tgcgcagcgt tacggaatgc cccatgtgag gatgcacctg cataagctca
    43921 tacccattgg tgcgggtttg ggtggcggct cggccgatgc cgcatttgct ttgagagcct
    43981 tgaatacctt tctggacaag ccgcttagcc atgaaaatct tttatcgatg actttggagc
    44041 taggtagcga ttgcccgttc tttgtgcata acacgcccgc tattggcagg ggccgcggcg
    44101 agcagcttga gcccataagc attaacctaa ctggttactg gattttgctg gttaatccgg
    44161 gtatccatgt aagcacaagg gaggcctatg agggtagcaa tcccaaaccg tggaaaaacc
    44221 ccataggcaa cattgttaac ctaccggtaa gcgattggcc caacaaatta gttaacgatt
    44281 ttgagaaaac cgttttccca aagtatccgt taattgaaag ggtaaaggaa gaactttaca
    44341 gcatgggagc ggtttacgca tccatgtcag gcagtggctc tacagtgttc ggaatattta
    44401 aaagcaaacc tgtaattaca gatgcattta aaggttattt tacgcataca tcagaaatgt
    44461 aggttaagcg gattgggtga tgattggtag aaaaatgttt ttttattgga tacagtgagt
    44521 cgatttaatg ggttatatgc tcagtgcaat ttctcgacaa cagggtatac atactttaga
    44581 ctattgcggg gctaaaatgt ttctcctaat tgtctgaaca tttctattgt gtttacatac
    44641 gaaacattca aagcaataca agcatcaggt attttcactt tattctgtct gttgggttca
    44701 cttacttctt gtgtaacaac aaacctgtta gcattgtcag caaggcaata agccacaaga
    44761 aaagcatctg cttcgtccgc attcagaaat tcatttaatg cattcggtaa ataatgtcca
    44821 cttctcgaca ttgcccaagc tgtcacctgt gcataagcag tcattacaac tgatgtgtct
    44881 ttgaaaaagt cttcgggcaa attatttctg caccattctt cgagtgcatc gtttctatcg
    44941 taaagctctt tttttacctt gtcaatactg ataaccctgc cttcttctgc caattgtcga
    45001 accttgttcc aaaatcccgc agcaacgtca atcggatagt gaaaccgatg agcctgaata
    45061 aaaaagttgc tatctaagac atatactggc attcgggcta caagtgttta gagaagaaat
    45121 tttcaaacgt gtcgcccttt aagcccgtaa gtttataggc atcacgatat aataatttac
    45181 cagattttac agcctgatta acatgactgg caaatgtgat actgatacgt ttttttgttg
    45241 ttgcgtaaaa gtcgccacca gagccttgac tttctttttt ggcaaattcc cgattggaat
    45301 actcttcata gaaatcaaaa aactgtggtc tgctgatttt gcctgtgtct agagccctgc
    45361 gtgcaataac tatttcactc actttaaaat aacgggaagc ataagcgaag ttgttcggtt
    45421 tgtgattcca tacttcatca aactcttgtg cagggactaa aaattcagca gcaactttat
    45481 cgcaaagaat ttcaatcggg tcgttggctg gttgtaacct tctgaaatcg aaacctgcac
    45541 tgtgacctgt ccaaatatgt gctaactcat gcacaatggt aaacatctga gcagatttga
    45601 aatcggaatt gtttacgaac atgaaaggag catattcatc caccaaaaca aaacctctgc
    45661 actcgtcaac aggaatgggt cggtgggtat tgttttcaac tatgccgttg aaaatggtaa
    45721 tgattccttt gtcttcaata tgcaaaacca aatggtcttg ggcatcttgc caagttctga
    45781 attgacttgc ccaattttca gttagttcca gggtttggcg aatatctgca actatagcat
    45841 ttacattatt gctgtttcta aactttccta caaatggtaa aggatgaaaa tcattatctt
    45901 ttaaatagtt tcttaaccaa tcctgccttt gctgtaagag caaaatggta tcaaacacat
    45961 tgatgctgat acggtctgct tggtttccgt tggtgcggaa atacggaatt ggcagttttt
    46021 cttgtggtgg ctgtggcaaa aacagataac caaagggcag gtaaactttc ttagaaaatt
    46081 cctccaactg tttcactgtg ggtttctttt gcccttcgag ccactccaat attttaggaa
    46141 attttacggc aaatgtaggc acatcataac ccgcccgggt aatagcccag gttagcatgt
    46201 tggtatttac attaatctca tttgccatac tgcaaagtta ttcatttccc atattttacc
    46261 tgcggctcgg ctgccatgga taattgttct tttgcttcgc tttttacttt cacttggcca
    46321 ttcattagca tgggcaaaag aaagtcacga acttccatta aatcaatatt ggtcattcgg
    46381 ttttttacaa tcaaatcaag aatcggctca attctttctc cacatttaag aattaacgaa
    46441 tcatcatcag gaactacaat tctactttct ttgagatggt ctattgtgat atgtcccatt
    46501 gtggtttttc tattctcagc cattaatttg aaatgattca aatagtttga aatctgatga
    46561 tagaaatagt atctggggta aaactccgat gttactttga aaatatgttg atttagtgct
    46621 ccaactttat cagcccaaat catgacatta aggctcgcac tccaagagaa aagtacatct
    46681 ccttctcgaa ttaagtatgc atcttcaata tccggtctgg cgtattcagt tgcagctgaa
    46741 taaccttcat tcatttcctt gattttaatg acaggcaatt tttcatcacc gtttggacga
    46801 tgcttttgca ttgctaaacc attcgtataa atagcaatgt catataagct tttagttttc
    46861 catcccttcg gtatctccat tttcaattcc tcattccatt ccatttcacc gccactggat
    46921 ttatagggtt tgccttcatt gttgggaaaa tcaaactgaa caaaccaata gttgtaaatg
    46981 gtcttggcaa ggttttcaag ctcggtgttg attcttttat tcaaggctat cttatcatca
    47041 ataactgtga gaaaatctgc tataagtttt tgctcagctt tttctggaac tctaatctct
    47101 tttagataag ctaaatctct gcttaaacct ggcactgtac tatgggaatt tagtttatcc
    47161 aatccaatta gttgaagata ataataccaa aatttaaggt cgtctttagt attgtctttt
    47221 aaagcaacat aataagttgt atcagttggt gtaaagtttt ctgatgagta ggtaacagca
    47281 cctactgttc cttttcttcc aataataata ccaggtccct ttaccttgaa actatcaata
    47341 aaaccaatga ttccattaga accatatact gggtatttac cttttggttc attgtcagtt
    47401 ctaactgaaa atccgtaaat taattctact ctatcgccaa gcaactcttt tttcatttgt
    47461 tatttttttt ataatcaagt ttattaaagt ttttcaaaat ttgtatttct aattctcttg
    47521 aattattaaa ataaaaagag attttgtttt taaactcatt tattctctct tcaaattctt
    47581 ctggacttag ttcaacatat tcaatcttag tttcaaaata ttgtcctgca ccaaaggaat
    47641 atgctttttg tttcatctgt tcattattaa caatcaccga aaaatcatcc aattgcctgt
    47701 atcccttaat ggtgtctatt atccgttttt catcttcatt gctcaataag gttttctggt
    47761 tcttaccgtc ttttactttt gttcctaact tacttgcatc aaccagtata gccgtatctc
    47821 tcgttttact tttatctata aaaatgaccg aaacgtttgt tccggtagta gcaaatatgt
    47881 tgggcggcat ttgcaccaca gcgtgcagcc agttactatc cacaattttt tttctgatag
    47941 ctaacgcaat ggcggctttt tcggtacaaa aaccggtagg taccacaatg gcggctttcc
    48001 ctttgtcgtt caacgaatac aagatatgtt gtatgaacaa ctgatagata gccatggact
    48061 cttttttctt ttttggaacg ctgggaacgc ctgcaaaaaa gcgttttcta tgttggtcgc
    48121 tggcaagggt ttcacggtca tcgctgaaat ccattttgaa tggtggattg cttacgatgt
    48181 agtcaaactt tttcaggtcg tttttggcat cgtttacatg gtatggattg gtaagtgtgt
    48241 taccttttat tacattatgc agcgaatgca ccagattgtt caatatcaaa ttcaagcgaa
    48301 gaaactctga tgatttttgc gaaatgtcct gcgaataaat ggtgcatttg ttttctccga
    48361 tttggtgagc cagcgccagt aataaacttc ctgaacctgc tgcggggtcg taaacagtaa
    48421 catttctggt gggttcaggc actaaaatct tcgccatgat ttttgccacg gccggtggtg
    48481 tgtaatactc ggcatactgt ccgctgtctt tgttgtagtc cttaatcagg tattcaaaaa
    48541 tggtagagaa aaaatcatat ttttcttcaa aaattggctc aaagctaaac gctaccagtt
    48601 tgttgataat ggctttgcag aaaggtgttt tttgattgct ttcaatgatg taaggggaaa
    48661 ttctatcaaa caaacgaacc ctttctttat caatggttga aacagaataa agttttatgt
    48721 tcaaatcggc aatttccagt aatgttttgt cgaacaattc gtgaaaattg ggctcgttct
    48781 gtttattgaa caaatagctg atgaaatgat gcttttgcag tttggcgcta cgttcaccga
    48841 ttttttccag catccattca tagtcgctgt tactcatttt ctggatgtcg gtttcaatgt
    48901 tggtgctgtt ctttaaatct ttattggctt tccgtgcttc gtgcaggaat ttgtcgttca
    48961 gaaatttata caggaaaact tccgtaatga ttttgtattc attcccggaa ttagccaaac
    49021 cgtaattgct gcaaacggtt ttaaggccgt ctattagatc ttttgttttt tgagtgtatt
    49081 caaggtgttt agtcatagaa taatttattt tattattccc aaagagattg caggttgata
    49141 ggtgttgcaa tagggtttat tgtgcgaccc cgcaggggtc gtaggtaatc gattgtcgct
    49201 gaagttatag actttaatgc cttcggcatt agggctacat cgtcatctaa tcccgaagag
    49261 atttcaggtt tataggtaat gcaatatggt ttgcaatacg accccgcagg ggtcgtaggt
    49321 aattgattgt cgctaatgct atagaccttt aatgccttcg gcattggggc tacataatca
    49381 tctaatcccg aaggggtttc aggtttatag aaaatttgat atgcgaaatg gtgcgacccc
    49441 gcaggggtcg tagatggatg aatgatatat tttctataaa tctttgatac cttcggtatc
    49501 acattattta acccattcaa ataaaaattt atcatcatgt tcaatttcaa attttttcaa
    49561 aaaatcaaca tattcttctc ggaaactcac ttttcgatga tgttgtttct gattcattat
    49621 atatttaaca acattatcaa tttgcgaatg gctatatgaa aatgcaccgt atccttcctg
    49681 ccaactaaat ctgaaattgg agagtttatt ttcatttata aactcgtttg atgccttttt
    49741 aatctctctc actaaatctg ataggcaaca agatggtttc ataccaataa aaatatgaat
    49801 atggtctggc attccattaa tagcaatcat tttctgttct tttcccctta caattccagt
    49861 aatgtattga tacaatcttt cttcccaatc agaataaatt aagcttcctc tgcctttaac
    49921 tgcaaaaata atttgaatat atatctgaga aaatgttcct ggcatattta tatggctctt
    49981 tgatagaaat attcgtttac tacagcgttg ttgaaaaatc gaactacatt caggtcacgg
    50041 atgcctttat tttccaatgt ttccagaatg gtacgtttag taatttcggc aaaataatct
    50101 gggttgttaa gtattgcctg attgtttact actgttttgt cggtttcatg tttaatgaaa
    50161 atcaaagttt catgtaatgc taaatcgctg ttcagtacat tcatgttttg ttccttgatg
    50221 cgtttgtgaa tgcgggcaaa tttgatgtcg ttctcatatt tggcggccag cattgcatct
    50281 ttattgttaa gcgcattggc ttgttcgtaa atctttttca aatcattgat agcctcatcc
    50341 atctcctcag atgttaattc ttcaatgttc tttttcttga aaagcctttt tagctcttca
    50401 aaaagagata taaatttcgg gtctttttta tcaaaatttc tctctagttc ttttcttgtt
    50461 ctttccagtt cgcttctgaa tttatcggca atttgcaatt catgcgtagc aattttcctg
    50521 aatgtaaact gcatgttttc aagtgctaaa ttcaaaagat ttgtattgtc cgctgcgttt
    50581 tctaaattct ctttcaggtt aaggatatca atacggtttg ccacttcatt atacaacttg
    50641 ttcaccctgt caaatgagaa tttgtctaac agttcagtgt aacccattat tttgatgatg
    50701 ttgtataagc tttttaagtt ctccaaacac tttttcagtt cgataatttc ctttttgtca
    50761 gttatctggc ttacaatttg ttgaaattct tctatattag aaaagtcata aagaaatagc
    50821 ttttctttga tttcctgaat ttccttgtct atttcttcgg cagatttgaa aaggttggag
    50881 tagttcttag tttcatcgcc caattcttcc tgtaattctt tgaaatactc cttgttggtt
    50941 ttatcaaatt ctttgcgaat gtcggcaaag tcaaccacat agccgtattt aaaattctta
    51001 taaggtcggt ttacccttgt tagtgtctgt aaaagattgt gttctttcac aacgcgggtc
    51061 agatacaatt ttttcagtcg tttggcatca aaaccggtaa gtagcatatt gtaaaccacc
    51121 aacaaatcaa tttttccttg tttgaaatcg gtttgatgtt ccttgcggat ttctttggtg
    51181 tctatatcat gcagtattaa agcaattgaa attggagcca actcaggtgt tagatagccc
    51241 actcccgatt cagctgcgat gtagtaatca gtttgtggtt catggtatac agtctgttgg
    51301 gcttccagtc gtttattgta tttctgaatt tcagtaaaaa tcattttcgc ctggtcagaa
    51361 gtgtcgcaaa caatcattcc cccaatactt tggtcgttgt gcaacactct gctttttttg
    51421 aagtcagtaa tgatgtaatc aaccaatggc tcaacaaatt tgggatgagc aaaaatttgg
    51481 cttttggtga actcgccttg cttcacaatt tcatcataca cctgattgat ttcagctttg
    51541 aatttggttt caatggcttc ccttataagt ttcagcgtat agccatccgc aatggattga
    51601 ttgtaaaagt atttatgaat ataatcccca aaaatatcct tgcttttata ttctttggaa
    51661 atgataggag taccggttaa acctattaaa acagcattac gatcggaagc aattaggttt
    51721 gaaagaaaag aaccctgtgg attataactt cggtgaactt catctaaaaa ataaattcgt
    51781 tgaatgttca aatcgtaatc agaaactttt gagatggatt cagcagaaaa cttatgaatg
    51841 tttacaacgg ttatggtttg tgcacctgtg tttcctgagg ttgctcctgc agttttaatg
    51901 ttttttatga agtcctcttt ggaactaacc atatcgactt tcaatcccct tgcttcaaat
    51961 tcgtttttgg cttgtgtggc taagtccaaa cggtcgacca caaaatagaa cttggctatt
    52021 acattttgct tttgataata gtctttcagg taatttacat tatagtatgc caaagctgtt
    52081 ttgccgcttc cttgtgtatg ccatatgatt ccttttttta ctcctgctat taattttttc
    52141 tcaatagcta aagttgcaaa caactgcgga taccgcatga tgtgcttttc aatctttgtt
    52201 attccattgt taacggtgtt cacataagca aagccataac gaagcaatat ttttaaacgt
    52261 tgtctgctga atagtgatgt taagatacgg ttagtaggag agtttggacc ttttgccgtt
    52321 tcatactcct tagttccaag aatggaaaca atgttattgt cgagcagtat ttgtttctct
    52381 atttcgctgt cctcttgcgg aacattatga attaatgaat tgtcttcttc tctgaaacag
    52441 ttgaaattta cctgttcagg gtcaggtgtt gcgtaaaatg ctccctgtat cggggtgatg
    52501 ttttcttcgt catactcttg attgttggag aaaaccaaca actgagtaat gttcatgaac
    52561 tttttgaatt tcggattttt gaatcgtgtg ttgattctgt tccgttccgc taaaattcct
    52621 tcacggttat taggtttctt tacttcaatg aaagccaaag gcattccgtt aatcaataaa
    52681 atgatgtcag gtcggaattc ttcttcgtca tttttgtaag tcagttcggt tacaacatga
    52741 aaaaggttgt tgtcgaaatt ttctaagtca atcaatttgc aggcaaagtc accttgcaag
    52801 cttttataaa atgcttttcc aaggtctgaa ttgtccagct ggatattgat ttcagaaagg
    52861 aaagtttcaa tttcactgtc cgaatattca gttccgttaa tctttgaaag accttgtttg
    52921 aacaggtctt tgaaaatatt tgtgtctgca tgaagatttg taatgccaga ctttggaaca
    52981 taagtaaatc ccaatcgtgt caaatgcacg atagcaggaa tttttactct tgaatcttca
    53041 ttaaaggtca ttttatctct taataattta atagtacaaa atacggtttt tatgagtgaa
    53101 ttagtaaaaa aggttctttt ggtttagcta taggcttgct ttagtgcttg tagaaatcaa
    53161 atgcgtcata tgtgtgggaa aatttttatc attgtcgttt cgttatttgt tcacttctat
    53221 ttttttgttt acagtttatt agttcatatc cttaagaatt atactaatac acctatgatt
    53281 aagtgtatag ctatgattgg aactagccct gtttcatgta actaaagtag taaaaaatta
    53341 aattcataaa ccagccactt tgtttttata tatgatttcg ctttgcataa aacgcataac
    53401 ggcttttgaa taacaccctt tccgtaacaa aaaaacctcc gcattgcgga ggttttttat
    53461 gcttagaatc ggttttactc aatgcttacc agcagggcgc ctttgcttac cctgtcgccg
    53521 ggttttacct taacctcttt cactttcccg gccgaggggg ctatcaggcg gttgtgcatc
    53581 ttcatggcgt caagtatcac cagcacatcg ccttgcttaa cctcctgacc ctttttaacc
    53641 tttacatcaa taatggttcc gggtataaag gccgtaacgt tcttatcgtc gagttttgga
    53701 tgtatcatag ctacaagttt tttagtttac aaaaggattg ttgggttgac taaaatggag
    53761 gaataccatg tttcttgaag gggcggtcgg caaccttagt gctggaaacc tcaagggcgt
    53821 gcagcagcag cttacgggtt tcggccggtt caatcacggt atcgatatag cctttggctg
    53881 cggctacgta tgggttagca aacttttcct tgtactcttc aactttttgg cgacgcatag
    53941 cttccggatc gggtgcttcg gaaatctcct tgcggaagat gatgttggcg gcaccctcgg
    54001 ggcccataac ggcaatctca gcgcttggcc atgcaaatac aaaatcggca cgtaggtggc
    54061 gggaactcat ggctatatag cctccaccat aggctttacg cagaattact gtcattttgg
    54121 gtacggttgc ttcgctgtaa gcgtaaagca gcttggctcc gtggcgtata acgcctgcat
    54181 gttcctgatc gataccgggt aggtaaccgg gtaggtcaac cagggtaacc agcggaatgt
    54241 tgaatgcgtc gcagtaacga acaaagcgtg cagccttgtc ggagctgtca acgtcgagta
    54301 caccggccat aaccagtggc tggttagcca caaagccaac ggtttcgcca ttcagtcggc
    54361 caaagccaat aacaatattg gcagcccact tctcctggat ttcgaagaaa tcggaatcgt
    54421 cggcaatggc acggataaca tcacgtacat cgtagggctc ctttggatcg cctggtacaa
    54481 tatcctcgat attgaagttt acctttggtt ctttgggctc aaacctgcga gccttgcggg
    54541 tattgttcca gggaatgtag gtgataagct ttttaatctg ctcaaagcac tcgtactcgc
    54601 tctcagcgta gaaatgtgcg ttgccagtaa tttgggcatg aacctttgcg ccaccaaggt
    54661 cttcctggga gatttcctcg cccagaaccg atttgataac ctcggggcca gtgatgaaca
    54721 tctttgagat tttatcgacc acaaacacaa agtcggttag ggcaggggag taaactgcac
    54781 caccggcgca agggccaagg attactgata tttgcggaat aataccgctt gagagggtat
    54841 ttcgccagaa tatttcgccg taaccggcaa gcgagttaac cccttcctga atacgggcac
    54901 cacctgagtc gttgataccg attaggggga tacgcatgcg gagtgcgtaa tccatgattt
    54961 tggtaatctt acgggcgtgc attgaaccca gtgaaccacc ggcaacggta aagtcctgag
    55021 cgtaaaccgc cacaggagca ccaaaaatgg ttccggtacc aataataaca ccatcgccat
    55081 gtagaacctt accatccatc ccaaagtcgc gggcatcgtg ctccacgaac atgtcgtact
    55141 cgttgaacga gtcctcgtcg aggagggcaa taatacgctc acgggcagtg agcttaccca
    55201 tagcaacctg cttggcaacg gcctgttcgc caccaccctg cagaacctcc tctttgcgtt
    55261 tttttagctc ttcaattttg ttttttaaag gcatagctgt ttttgttggt tagtaatttt
    55321 tgccaccatt accatgcatc gtggtaaagt tttgtgcaag gttacgaaat tgggtgcaaa
    55381 tataaaagaa acgggccgtt tttttcgacc cgctaaaatg tattagccta aatatcaaaa
    55441 tcctacacca gtaaacacct gtttaatgtt tacttgggtg ccatcctgta aagacccact
    55501 gcaatgattg caaacaggaa gttgggaacc cataccgaga tggctggtgg tagatgtccg
    55561 ccaacggcaa acatggttga aaatcgcatg aataggatat acgagaagct cagcgccagc
    55621 cccagtccaa tatgcaggcc tatgccaccg cgtacctttc gcgatgaaag cgatacgcca
    55681 atcagggtaa gaatgaacac cgaaaagggg taggcaaatc gcccatgctt ttcaattagc
    55741 aggtattcaa tggtatcggc gccctggagc ttttgttccg ctataaaatc gtttagctga
    55801 aataggttca tggtttcaat gatcttcaac ctctggttga agtcggatgg ggtgaggtag
    55861 aacgtgctat caattttatc gcctttgata atttcttcgc cactgtcagt ataattacgg
    55921 atgtagtagc ggtttatagt ccatttctcc ttggtggaat cccactgtgc attatcggct
    55981 atgagtttcg ataccagctt attcccatca aatttttcaa tggaaaggtt gtaggcggtg
    56041 ttgttgtaag tgttgaatga gcgcatgaaa acaaacattc ccggacgcac ctgtcggtgt
    56101 atgttcctgt cggtattaac atagggtcgt ttgatgtact tgttctcaaa gtctaacctt
    56161 acagcattgg cgggtggaat tataaagcta ttgagcacaa atgagaaaat ggcaattatt
    56221 gttgcggaga taaagtaggg gtataacata cgcttaaagc taacaccgct gctcaggatg
    56281 gcaataatct cagtattata ggccattttt gaagtgaaga aaataacggc aatgaatacg
    56341 aaaagggagc tgaacaggtt ggcaaagtag ggtataaagt tgaggtaata cctgaaaatg
    56401 atatcgtaca ttggggcatc cttctccaga aagtcgtcaa ttttctcggc aaggtcaaat
    56461 attactgcaa tgccaactat tagtattatg gcatagaagt atgtgccaat aaactttcgg
    56521 attatatacc agtccagtaa cttcatagag gtgttttata gacgtttcga cactttttta
    56581 accatagcgt ttttccattg caggaaatcg ccctcaacaa tatgttttcg tgcttccccc
    56641 actaaccata agtaaaaccc taggttgtga atgctggcga tttgtgggcc tagcatttcg
    56701 cccgcaataa aaaggtggcg gaggtaagcc tttgtgtatc tactatcaac aaaagttagg
    56761 ccaaaaggat cgataggtga gaagtcggct tgccatttct tgttccgtat gttaattata
    56821 ccctccgagg tgaaaagcat accgttacgg ccgtttcggg tgggcattac gcaatcgaac
    56881 atgtcaatgc ctagggcaat ggattgtagg atgttctcag gcgtacctac gcccatcagg
    56941 tagcggggtt tactcttggg cagtatgccg tttaccactt caaccatttg gtacattacc
    57001 tcggcgggtt cgccaaccga tagtccacca atggcgtagc catcgcggtt gtacttctgt
    57061 acattttcgg cagcgcgttt acgcagatcg gggtaaacac agccctggac gattgggaaa
    57121 agggtttggc tatgcccgta aaggggttcg gtggtgtcga acctttgaac acagcgttca
    57181 agccatttct cggttaggtc gagcgatttg cgggcatact cgtaatcggc attgcccggt
    57241 gggcattcgt cgaatgccat tacaatatcg gccccaataa tgcgctgaat gtccatcaca
    57301 ttctctgggg tgaacaggtg gggcgagcca tcgatatgcg agcggaacga agcgccctgc
    57361 gctgttagct ttcgcatgtc ggcaagggaa aatacctgat atccgccact atcggtaaga
    57421 atgggtctat tccagctcga gaattcatgt aacccgccgg cttgctttag tacatcgagt
    57481 cccggacgta ggtaaagatg gtatgtattg cccagaatga tttgggcgtt aatgtcgttc
    57541 tccaactccc tgtgcgaaac accctttacg ctccccaccg tgccaacagg catgaatatt
    57601 ggggtttcaa ttttaccgtg atcggttgtt atgatacctg cacgtgccga ggtttgtgaa
    57661 tcggttttga gtaacttgaa atccatctgc ttgtaaaaat ccgcacaaat ataacctaaa
    57721 tactaacagg ttaagggttt attttgattt gttaacggta aatactcagg ttttaaatta
    57781 atacttgcca atgtttatta accaggttgg ctcctggttt atgctttcgc cggcatagta
    57841 aacgtgttcc aggtacaacc ccgatggggg agcagtaaac tttgccggaa gggttgagta
    57901 ctcactgaag aacgagtcga tatcggcctc cgataatttg cccagtccaa cctgaaccag
    57961 tgtgcccacc attcggcgta cctgcttcca aaggaagtgt gaccccacga tatgtatgag
    58021 aattgaatcg tcaacctcaa atatttttag gtggtcaatt tttacaaggg tcgattctcc
    58081 ttccttttca ggtgcgccaa acgatttgta gtccttcatc ccggtaaagt gatgggtaac
    58141 ggctgccata gcctcaacgt taagcggttc cttaacccac cagcagtagt ccttgccaaa
    58201 ggctgttcgc ctgcgcgaaa tgtggtacac gtagctgcgg tgcttggcat cgtagcgtgc
    58261 gtggaagcga ggtgaaacct ttgccatatc aaggatattg attgatgcag gaagaacctc
    58321 attcatcctt tccatggcaa cgtagggtgg cattgccgag tcgatatcga ggtgggcaac
    58381 ctgccccaga gcgtgcacgc cagcatcggt acgtcctgct ccgtaaagct catagctgct
    58441 taccttgtaa acctccttga tggcatccat tagcttgccc attactgtgg cggcttcgcg
    58501 ctggaattgc cacccgcggt accttgaacc gtcgtactct atggttaact taaatcgagg
    58561 catttgcgat ttttctttga aatttttaca aagttacgct taaaatgagg tataaaaaaa
    58621 gtgggtaagc tacttatatc gcaccgctac aaccgcttac ccttgctacc ttccggtcct
    58681 gggggggttc agcaggagct ggtcgtataa gacttacccg ctgcaaagat agctaaactt
    58741 tacaatacct gttgaaccta ctagcttttt ttagcaaaat tcatatattt gcaatgaaac
    58801 taaaccgaat acaaaaatgg aaaaacaagg aaaccctatg cttaagcatt caatgaatta
    58861 cggtttaata atgggtgttg cactcataat tctaagcctg attacttact tggcaggtat
    58921 tgtaaaacca ccgttttggg ttagcataat aaactatgcc attataattg gtattatcgt
    58981 atggggtact aaaacgtatc gcgatgaggt gttagagggt gcaattacct atggtaatgc
    59041 cttgggcttt ggtgttttaa ttacactttt tgcgggagtg attgtggctg tatttactta
    59101 tattcaaatt acccttattg atcccgactt tgtaagtaag atgttagcca ttgctgagga
    59161 ggaacttgta aagaggggta tgcccgatga gcaaattgaa atggctgtag agatgcagaa
    59221 aaagtttatg actccgctaa tcatgactat ttcatcgtta ctcggaatgg ttctgatggg
    59281 attcatattc tcactgatca cttctatctt tctgaagaag gaaaaatcgc cttttgctgg
    59341 tggcggtgaa gttattgaat agtaaccaaa catcaatatg gatatttcta tcgtagttcc
    59401 tctgtacaac gaggaggagt cgctccccga gttaatggaa tggattgccc aggttatgac
    59461 ccagaaccat tttacctacg aggtaattat ggttgacgat ggtagtaacg ataacagctg
    59521 ggcggtaatt gagagcctga ggctcaagtt tccatccctg agggggatta agtttctgag
    59581 gaactacggt aaatcggctg ccctgcaggt tggctttaca gccgctcaag gcgatgtggt
    59641 gtttaccatg gacgctgacc tacaggatag ccccgatgaa attcctgagc tttaccgtat
    59701 ggtcaaggaa caaggctacg accttgtttc gggttggaag aagaagcggt tcgatccgtt
    59761 gcttaccaag cggatcccca gtaaacttta caatgccacc gtgcgtatgg taagcggtat
    59821 aaagctgcac gatatgaact gtggccttaa ggcctaccgc cgcaatgtgg ttaagagcat
    59881 tgaggttta
//
LOCUS       DAOOWJ010000003        52444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_682_length_52444_cov_7.958255, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000003 DAOOWJ010000000
VERSION     DAOOWJ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 52444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52444
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_682_length_52444_cov_7.958255"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..5362
                     /locus_tag="PLW22_00655"
     CDS             77..5362
                     /locus_tag="PLW22_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU73238.1"
                     /translation="MKKILAFIFLSFSLTANSQPKKIKLQADDIQIGKDYHEKFYATS
                     QKSYRLKLKIDALEWDKQQAKTGETFTKLWFKNSLPDGNIGEPEIPVVKKLICIPLGA
                     SVSARVSKFKMDEVQLSAKGIQYPLLPVQPSARKDQDTLQLPFHLKPEAYRKSTFQSV
                     KPEVLVEVMGNLRGYTIARLTVRPVEYNPAHQKIRVFSDVEVDVDIQEADTKSASKTN
                     PYYSPYFDVVYKSMLNAGGATYDEHPDLTRYPVSMLIVSHRMFETALQPFVEWKTQKG
                     FSVTVKYTDEIGATSDAIKTYIQGVYNSATPENPAPTFLVIVGDVEQVPASATGSQSG
                     KLTDLYYASVDGDMFPEMYYGRLSATSKAELTAIINKILYYERFQFADASYLNNSTLI
                     AGADASWNPAIAQPTIKYATATHFKPSNGWSNIYEYGITNDPNNPSASSGYTGCYDPE
                     RIAVGFINYTAHCNETDWQDPALTISAVEAFINNQQYPFVIANCCLSGNFGHSESVGE
                     AWLRKANGGAVTYIGSSPNSYWKEDMYWAVGAFPMSGDNNGYVPSFEESTTGGYDAPF
                     VSAYTTAGAIMFCGNLAVTQAELNDYSRQINSTYYWEAYNILGDPSLMPYFKIPEANS
                     ITFPATTAVGVSSVSIGAKAQTYVSLSRNGQIIDTKYYPTDDLLDFDIPGQAEPGKIV
                     LTATRPQTQPFIDTIHVFVAENAYLTLASTTIDDSEGNNNATADYGETIKVNLLVKNV
                     GKTNATSIGAKITASTGLIELTSADSIGIGNIDAESEKWVNSAFTFSIATNIDDKYSQ
                     VFPIIFTSTERSWTSNLRITAAAPKMVFNGYTVVDTLMGNSNSQFDRNEVVDLKFEFQ
                     NKGGSAIPDFSAQATIPDSLLNAVSVNFEPISNRTIQPNEKAILTLRVSTSPNLIHDT
                     IPIDIDYSSVSYSNASDQFTSKVPVLLFGTTKMTNGTVKTCGTIFTDTGGETANYNNG
                     ENYTLTFETLSDIQRYSVEFISFKTELGYDYLHIYDGAGITSNAITGSPFNGNTGPAP
                     FFTSGNSVTFRFTSDGSTTASGWKAKLECITPQQLPKCIENPKPGNNELNVEYNRLSW
                     RTTSDALFYDVYIGTNPDSLVLLKRVTEAYVDIPLMPQTNYYWRVMPGNHLGLCDKPC
                     EVWNFTTASVIGQVLMTNSTVEVDSLWFYDSGGPTSNYGNNENYTITFKPRNNGQKVK
                     VQFMEFDVESHSTCSYDYMIVYDGPSTQSPELGKYCGDNMPPAYTSTSANGELTFKFV
                     SDLNTVGTGWKAKITTTGSITTYPVTFNVKNNGSAIPCAAVSTADGIKFTSSSGSVNF
                     SLPNGTYSYTVRANGYEPVLGSTQIAGSAKTIDVNLTKHDTIQLLVRSALDLAAIPLA
                     KILVNNQTYYTSNLGMATIYIPAGDYTFNLTADGYYAKDTIITVQAGGGNHNINLLPV
                     SYNVQFTISDINGNKIPNAKVFVDTLLGETNSEGLASFTVSRGLKTVSIQKEGFVSTD
                     FWMELSSDSSVNVFLTPVYGDVHQVMFSITGTGPKGTWPLDNAFVKIYNGTQLYHQGK
                     THNGVATLYLPNGNYSYEVSLEGYQGTEQTEFTVNSNPIEIPVLLNQLTYSITFDVQS
                     GGSPVANATVKINGYSDQLTDANGLATFNQVGYEKGLQFSVLHPNYYTISGTLDATKS
                     ETISVNLTPTSTDIVDPDKNLSMYPNPATSHIRIEANETIESVSIVSINGTLMHWEQV
                     NNSRHTINLNLPTGVYVVRFTMSNGRLYYRKLMVH"
     gene            5469..8378
                     /locus_tag="PLW22_00660"
     CDS             5469..8378
                     /locus_tag="PLW22_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73239.1"
                     /translation="MLRFFRFFSLHKFALALSLVAITTLSVVVWQLAQNIDRQNIQTI
                     SADLRSANNDVIRDIERSFWDIKLADYQNDDDNEIIFGKNINLEESSLFNLDNNKLFG
                     TNVLLLHNNRFYAKALFKTSTHEFEYWYPIARTFNLYNALTGNRLAILTDNENTLLSQ
                     PLTLARAKNLTGDKTALIAAEGVKSNSWVLLVLPLLLLVVATAVIYSLLKRSNCRFSG
                     TNSSGKTVHNNAREVLAQINLKSALNNSNVALRVIDTNFRVIFQNQEYEKLTSVTLSK
                     PNHPLCHESFGSIYCHTDECPVRSILNGAKEVKREETRFLPTGKKILVELHAYPLKDD
                     YGNTVAVAEEIKDITGVHYTEEILRQTENQFTVFIDSLPFGVFIEDGLTHEIVYQNFY
                     LKQLTCNTGFKEFIKGAEIIGVDADNNVTEFQLMDSSGIMRYFNLHQFKFLGVNNSLK
                     IGGLFVDNTRKKEIEHYRDVLSKAIEFTPVSIAILSPYFEFEFINPNFTEITGLTIEN
                     LFSKSISDINLEYNGGKHIQKALDSVRAGKTWQGELQLNGKNRERIWVSASFTPVMFE
                     SKLQHILVILENITRRKEYEKELILAKNKAEESDRIKTTFLNNISHEIRTPLNAIIGF
                     SSVLTDPYLGQFERSGFSDLIYKNSQELLQIIENLLEISQIEAGEIKISKTEFSVNAL
                     MNEIYSTFEEQERGSSRIKLSLRKEIFQDDLVVLSDPSRIKQVLKHLLSNSFKFTTNG
                     FVEFGYRLKDENNLLFYVVDSGIGIEPDKMDLIFNPFRQADSSSTRRHGGLGLGLAIS
                     KHVVEKMGGKIWINSTPGSGTSVFFSIPFIPVKLKFENANTGNGLKEFNWDGKTILIA
                     DDIDANFVFFKAALQKTKANLIWARNGLEALNYVRNGEPVNLILMDLVMPEMDGFEAT
                     RIIKSINQKLPVVGQTAYPETISSNKLAECGFDTVLEKPIRTNQMLMEIDRFLVN"
     gene            complement(8989..10368)
                     /locus_tag="PLW22_00665"
     CDS             complement(8989..10368)
                     /locus_tag="PLW22_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOU73240.1"
                     /translation="MKLHSNFTIMLVAIAMMACSSPKDRVEVWVSKADSSMLLEQQPG
                     INFSNDQVGEVIDVDTNQLCQKVNGVGAALTWSSAYVFKNYLTPEARLQLFRELFTPE
                     GIGINVVRLTIGSSDFSQGNHSYSETPDTSLSNFSLDMDMGEVVPMMREILAVNPDIT
                     IIASPWSPPAWMKTSNSMVGGSLLRQYYGVYAGYLVKYIKTMQELGIPIYGITVQNEP
                     EFGTATYPCMLMSAQEQNEFIRDYLGPRMAQNDLKTRIILFDHNCDSPQYPISILNDS
                     VTRRYVDGSGFHLYKGEIEALCSVHDAHPDKNIYFTEQSGGGWAPNFGDNLLWYSETL
                     MVKAMRCWSKTVLLWNLALDENDGPKNNGCSNCWGVVQVASNGAVKRNAEYYALGHFG
                     KFVAPGAVRLVSNGQSVSNVAFLNPDGSKVLIAVNTSSQPAGVTVKCSGRQFAYQMDG
                     REVVTFRWR"
     gene            complement(10781..12247)
                     /locus_tag="PLW22_00670"
     CDS             complement(10781..12247)
                     /locus_tag="PLW22_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOU73241.1"
                     /translation="MKNPCPRPFSSAAGMLLACLFLAATLLSGCSDDSDSVDNPPDDT
                     TQLSDVRVWVTTCNQAYRFKEQSVSFSDDVAPFTIRIDTLNRYQQVDGFGAALTGSSA
                     YNLRRMTNSQRASLLSELFDPLKGIGLSYLRLSIGSSDFSLSLYTYCDYPSIDSFALH
                     PIDRRDLIPVLKEILAINPDLKIMATPWTPPIWMKTSAAWSGGSLIQDFYDEYAGYFV
                     RYIRAMAGEGIPIDAISVQNEPMWEFGTPGMKMTWQEQLTFIRNFLGPKFAAEGISTK
                     ILIWDHNWDMPDYPISILSDPVAYPYIAGSAFHGYGGDYSAMLTVHNQFPEKGLYFTE
                     ISGGGWATNFGDNLKWTVENVFMGTLSCFSKNVLLWNLMLDDHWGPFIQGGCSNCRGV
                     ITLNSDGSLTRFNEYYALAHFSKAIRPNAYMVGTTAVGDVSQLADFRYVAAQNPDGSK
                     VVVAMNFSSTGKQLAVRCGNNKFTFTVQPESLVTFMWR"
     gene            complement(12418..13770)
                     /locus_tag="PLW22_00675"
     CDS             complement(12418..13770)
                     /locus_tag="PLW22_00675"
                     /inference="COORDINATES: protein motif:HMM:NF027723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5016 domain-containing protein"
                     /protein_id="HOU73242.1"
                     /translation="MKIFKYACIAGLVMVALAACEDDGTKTTSQPAISNLSIIPTTGL
                     TYGDSIAVSATVSDEVTPLSTFDIQVIVADVEIYKQSIRTKGNNANVTAKFKLPFAAN
                     AEDNGEVTVKLQSINIDGFSTSQTETLHAARPILGDTLYLVTSNETIKLAKSPDNPFL
                     YLSPSGSYPNEIVGKIATSENLMAAEWVWVSSGNTIVIGSHTDSDIRLSDPSYSVEQL
                     SFNTMTFELKFIGEMLPSVKFNTTTLLIGEDGRFHGEVEFTKGEVVTVEGITNLAAAY
                     NPDFFDFKDGVLTFKAPSGKYQVVYWAGTINYMWLRQDNAVYPDALWLTGAGIAMPTF
                     INDVTNIWWGWDDWGVTTRQAYFFCPKVGENKYQTTVYMMNTEGSWWLQAEFKLFPSK
                     SWDNEFSSMDWGYISNNLTSNTNGNFVHTTELTEGLYRITIDMSGGKGNYFLIAQKIN
                     "
     gene            complement(13790..15271)
                     /locus_tag="PLW22_00680"
     CDS             complement(13790..15271)
                     /locus_tag="PLW22_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOU73243.1"
                     /translation="MKKLNGYLLAGVALLTFTVVSCEDFLDKDPISNYSNSTLGVDNN
                     NDTLKYNTAEQAEALLATCYSNFRGELFQLDYFVNGDMQSDDAYAGASGTEFSQFDNY
                     SIDATNKNVERDWKYLYKMVTDCNTVIVNVPKITDQALTDARKKEIVAEASFIRAWAY
                     FDLVRLYGDVPLVLDVVTTITADNVAEVYPLLYPKRDSKDLVYDQIISDLNTALDGVP
                     VTAPNKGYVTKGAVNALLARVYATRQPVDYTKVNDYCDAVINGGYSLVSNYDYLWDFQ
                     HENSSEAIFEIQYDGNWSTGTGNWAVSMFLGTGWKKFNTPSNDLVAAFDAENDVIRKN
                     SSIKFEDVTGSWTDANWPAATYPFVYKIRDEAGTSNIILLRLADFILLKAEALNELGL
                     PGADTLVNQIRSRVNLPPVSPTTKEQMRLIIEKERRLELAFEGIRWFDLLRTGRAISV
                     MENVRGGNGQLLYAGKLQPYRLLWPIPQEQRDLNSMLTQNPGY"
     gene            complement(15284..18259)
                     /locus_tag="PLW22_00685"
     CDS             complement(15284..18259)
                     /locus_tag="PLW22_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU73244.1"
                     /translation="MKYFLKIFRKGARIVLLGILCVYWIPSMAQTISVSGKVTDREGE
                     PMAGVNIVVKGTTNWTITDVDGKYNISAPENAVLQFSFIGMKSVEEIVGGRSIIDVVL
                     ESESLDISEVVVIGYGTSRKKDITTAVSVVSTDDFDKRPLTNVAQAIQGKAAGVQVVL
                     PSGKPGGDAIIRVRGATSVLAGNDPLFVIDGVPTTSMQALNPQDVETVQVLKDASSAA
                     IYGARAANGVVIITTKHAKTGVASVSVNSYLGFSNLANSLDVLNTEQYRELMDEIKGP
                     GTVPASQTNYTNWINETFSTGQIQNYQVSVSNGTEKMKYYFSAGFSDEKGIVKPAENK
                     KYSIRTNINSDLSKWLKVGTNISFIHNWYNDVSDNANSGRAGTILSAINTPPFLHIWD
                     PNKPEQYEVNPFQNSWENPLAYASRINQSKTERVLANANTEIAFLKDLKLKSNFGIDG
                     YIYQRDLYIDPVKTVYGRTENGIGEADKSNSLVWLIENVLSYEKTFGNHNLSVQAGQS
                     AQSSRWDQSYISAKDFPKNSAVTTINAANQITYAASSASEWAIASFFGRVSYNYMSKY
                     LFTANFRYDGSSKLSSDNRWDFFPSFSAAWRLSSEPFLKNVLFINDLKIRGGWGRNGN
                     QEGIGDYAWQALYGFSRQSPTNPLSGPGIYQMTLENPSLKWEVTYQSNVGLDLTVLDS
                     HLSFTGDVYWKKTNGLLVNVPLPSGSAAVDYITRNDGKMKNWGVELSLDAKDFRHELK
                     WSTNLFWSMNRNEITELQLSQIYNFAQLESNNEYVIRMSPGYSLGTFYGYVAKGVDPE
                     TGDMIYEDRNSNGVIDPDDRTVIGSAEPDFIYGLNQTLTYKGIGLTLFFQGTQGNDIF
                     NASRIDTEGMFDVKNQTTEVLRRWKRPGQITDMPRAVSDGSTFNVHNSTRFVEDGSYF
                     RLKALTLSYSLPSKWLSRVSIKNFTLYATATNLFTITGYNGFDPEVNYGGNSSVVQGV
                     DYGTYPQSRSYIFGLSFEF"
     gene            complement(18285..20594)
                     /locus_tag="PLW22_00690"
     CDS             complement(18285..20594)
                     /locus_tag="PLW22_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU73245.1"
                     /translation="MYRIRLWPLGITIMALIFGSCNVEKRSDAEQVAKAMLDRMTLEE
                     KVGQMAQITLDVVGKGDSLFNSYEPFELDTAELQEAIVDYHVGSILNSSNNRARSFTT
                     WNGIISTIQNMAVTKTRLAIPILYGIDAVHGANYTAEATLFPQQIGMAATWNDSLVRE
                     IGSIAAYETRASGIPWVFSPVLDLGADPRFPRQYEGFGEDPYLCGKMGVALIDGYQGV
                     DVSHPLKVAACAKHYLGYSVPASGKDRTPAQLPDIALREYHLPPFKAAVDAGVKTIMV
                     NSGVINGISVHASHYIITDLLKDELGFSGVVVTDWNDIENLYRRDRVAATEKEAIGLA
                     INAGIDMAMIPYRYKDFCNLLAELVREGSVEESRIDDAVMRILKLKVELGLFERPTTV
                     LGDYPKAGSEEFAKVSYQAAGESITLLKNSNGILPLSKDKRYKILVAGPNANSMRTLD
                     GGWSYSWQGEKVEEFAGRFNTIFEAIKSKVPQSEVSYIPGVSYPPYGRYYEEQYDKFE
                     ESAAAARKADVIILCLGENSYAEKPGDLNDLYLGSKQLNLAEELAKTGKPVILVLNEG
                     RPRVFSPIEPLMAAVVQTYLPGSYGANALADILFGDINPSGRLPYTYPSYPNSTVVYY
                     HKPAEEQKPNIGAYSYESDYNPQYPFGFGLSYTTFEYSQLATSKSEFRQNDTLTVTVN
                     VRNTGNRTGKEVVQLYTSDLVATITPDVKRLRKFAKIELAPGEAKTLTFKLSSSELAF
                     VDGNGKLLVEPGEFLISVGNLSAKFLIVN"
     gene            20812..21546
                     /locus_tag="PLW22_00695"
     CDS             20812..21546
                     /locus_tag="PLW22_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU73246.1"
                     /translation="MDDELRLIPNLSIDCVIFGWDEGVLKVLLRKELIEYKNIVHIEW
                     KLPGNHVGMNERIEDTATRVLKEQAGLENIYMKQFRVFSDLDRLRRREMDYRWIKKNG
                     LGEDRVITVGFYSLVNLLNFDSSKLLNVAEWHNVNEVESLIFDHKEILDEALKKLKED
                     VTMHPIVFELLPKKFTLTQLQQIYEVILGTRFDKRNFRRKISNLKYVVSCNEKQYGVG
                     HKPPSLFRFDAKIYSKIHQKNLDATA"
     gene            21564..21836
                     /locus_tag="PLW22_00700"
     CDS             21564..21836
                     /locus_tag="PLW22_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOU73247.1"
                     /translation="MVTEIIGLLAAILSTLNQFPQAYKVMKTRDTQSISLYMYCIVEV
                     AIALWLVYGILLRDFPLILANSLSLIPITYIFVVKLFHVISGKESI"
     gene            22092..22268
                     /locus_tag="PLW22_00705"
     CDS             22092..22268
                     /locus_tag="PLW22_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73248.1"
                     /translation="MVSSFWLKSSLNPSNSSTIMVVPETEISAVVELSSKDIFKTPNS
                     ITLNGYVYDMQKNG"
     gene            complement(22327..23451)
                     /locus_tag="PLW22_00710"
     CDS             complement(22327..23451)
                     /locus_tag="PLW22_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU73249.1"
                     /translation="MTQKVGEITALSDDSLQKLIREYRAIDMTSLTPNEKGFQEFIDS
                     NINDYNLDMDRDESMNPYQKLSKYILKKYFRAINPETEIAFVSGVNLAVFLAIASTVK
                     DGDSVIVFEPFTSNLKTAVELCGARPVYIPLRAPDFRIDWCEVQRAINATTKLMVISS
                     PHLLTGQTLTAEDFEELQRLINGTKIKLILNESMAELGFSSSEGTSVNFYPRLCQVSY
                     RIGSLNIPVALNDSEIAYCIAPEPLMGAYKNIHSAITSNPNLNRAEDFINILSAEIEG
                     HMLADFLDENYKLLLETLKDSKLIPVKQSAGYMVMLGYKGYADIRDVEMVEKFIAEKG
                     VGLLPLSGFYHDRQCHRYLAMNITIPRDEFKEALVRLASL"
     gene            complement(23550..24278)
                     /gene="recO"
                     /locus_tag="PLW22_00715"
     CDS             complement(23550..24278)
                     /gene="recO"
                     /locus_tag="PLW22_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOU73250.1"
                     /translation="MLQHNRVIVLRTVRFKENSLIVSCYAREHGRITLLVNNAFPKGK
                     KAGLNVYFQPLSILDVVYYYRPNAEMYRVKEVTPTYAFTSLHLNPIKLTITIFLSELI
                     YRTVREQETNQHLFAFIESSVQMLDNLNTGVANFHLIFMVQLSRYLGFYPLNRWSPEE
                     PYFDIKNGVFCGVEPAHGFVLEKQTSKLLSNLIDTPMLNPEMLSLNQNQRQILIEAIA
                     SFYRFHLGSGIRFRTLPILIQLFE"
     gene            complement(24283..26583)
                     /locus_tag="PLW22_00720"
     CDS             complement(24283..26583)
                     /locus_tag="PLW22_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOU73251.1"
                     /translation="MVKRIYILLSFIFVGICKVHTQPAIGQWRDYYAFNDARELSISG
                     SKLYCAVANGYFTYDTENGELNKYTTVSGLSEVDITALSAIPQKQITLVGYKSGNIDL
                     VYENTKRVVSLPFIKDKPMLGSKCINHFYYFSSLVYVSTDFGLVVIDPDRLEVKDTYF
                     IVENAGTLKVNRLVAWNGKFWAATSQGVYSASTNDPLLISYERWSREQFFTDPAAECR
                     GVTADETHLLALEKVGGNDILWMNSGSGWVEVDRPFDETLGVSIGFQKIAAFSSNAIQ
                     KYSLQGEKLELVSSYLFGGTPKVADCIPLENNALAVADEFNGLVIISPTDHSVHTPTG
                     PFSNKFFHIDASANMVVAASGAYDAAFGNFWNPFIAHTFSDGRWSYYADWFNHDAVRV
                     KADPRNPNVFFVASWGGGLYKLNSGTLAEHYNPDNSTLQSVFPGQPYCRIAGMDIDPK
                     GNLWVANSEVPNPISVLTAHGVWKSFPYYSAIGTSRIISLTVSPSGIIWLALARDNGL
                     FVLSPGNDVEAASDDIYLKFRPRDANGNLFSNEVTALAFDRDGYLWLGTNQGVLVSYN
                     PHRVLQGETSFQKIKIPDVVEGLAVYLLETEEVTCIDVDGGNRKWFGTAKSGVYLFSA
                     DGTKQIHHFNTKNSPLPSNTILSIKAHPVTGEVFIATDKGLIAYRSNASEPANTFDKV
                     YAFPNPVRPNYSGVITIAGLMDNSVVKITDIAGNIVYETRSNGGLATWDGKNRNGQRV
                     ATGVYLIFCSDSKGEQAAVTKLLFVK"
     gene            complement(26647..27240)
                     /locus_tag="PLW22_00725"
     CDS             complement(26647..27240)
                     /locus_tag="PLW22_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOU73252.1"
                     /translation="MAGSSFSLVFATNNPHKLKEVQHALGDRFTLVTLNAVGITDEIP
                     EEYGTLQDNALQKARYVYNKTGQNCFADDTGLEVEALNWEPGVYSARYAGDAKKPKDN
                     IRKLLSNLKGVENRKARFRTVIALILNGEEYLFEGVVWGKIIDQERGADGFGYDPIFV
                     PDGFTETFAEMPLSLKNQISHRGKAVEELCKFLKSRF"
     gene            complement(27248..28042)
                     /locus_tag="PLW22_00730"
     CDS             complement(27248..28042)
                     /locus_tag="PLW22_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU73253.1"
                     /translation="MTAKKSILVPTDFTEVAWFALQHAIRVSEVVKEPIHILHCVSEP
                     EKLAEAESKMASLIESVKSKYAVTPKSIVKVGNYMTDIASTADEINASMVIMGSSTVK
                     EMDEHKVSWVLKLITSCKVPFITIQEPPVNKRYDDIVFPVDFTIENREKHEWISYFCD
                     YYLSRFHIIKPNTSDPKLMAQIDINLASAKKFLDERGAKYVEYTVPGIKKYEEEVLDM
                     AVNIRADLIIIMTTPTSKDGKYIVEPEEQYILANARHIPVMSINPR"
     gene            28281..>29374
                     /locus_tag="PLW22_00735"
     CDS             28281..>29374
                     /locus_tag="PLW22_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU73254.1"
                     /translation="MMLYVRYYYTKKSFALQQKLFVRTEELLRERQKTENLLSRVLPR
                     EKAKELIDQGKVDAQKFQMVTVLFADIQGFTKITDEINADSLIDQLDRLFFEFDSIVE
                     KYGIEKIKTIGDAYMCAGGIPHPNRTNPFEVVAAAVEMMGCMQKLNQQLNNGKEIWEL
                     RIGIDTGPVIAGIVGRNKLTYDIWGTTVNIASRMESASEPGKINISENTYLVVRDLFE
                     CKFRGKIPIKNKGDVNMYFVEGFLPKFSESSRYIPNNDFRVELQLVRLADLEEFVLEK
                     LEKGLPKNLYYHNLKHTVDVYTQVELIGRAENIPNEDLLLVRTAALFHDMGHLIDYAT
                     HEEMSVKMAHEILPRYGYTPEQIARVAEII"
     assembly_gap    29375..29474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29475..29827
                     /locus_tag="PLW22_00740"
     CDS             <29475..29827
                     /locus_tag="PLW22_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73255.1"
                     /translation="PRYGYTPEQIARVAEIIIATKLPPKPKNILEEIICDADLDYLGR
                     TDFIPVSNALYKELHEHGMVGSLHDWNLIQIDFIQKHQYFTQTARRLRNVNKDVQLKN
                     LKRWMNELENDTSL"
     gene            29923..30762
                     /locus_tag="PLW22_00745"
     CDS             29923..30762
                     /locus_tag="PLW22_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU73256.1"
                     /translation="MDNIKKIILVPWDFTEGSETAFQHALQLAKAGGNEIMLLHIIPR
                     AWIIDSIYRKVNFKKIETYNAKIEKAASELEHKYGFKPQTLVLEGYPKRIIKELILTA
                     HVNLIVAYYAYKFGSKEHRISRYLKNLFFKDTTLPFIVVSKAPIHSHYIEIVIPVDYD
                     RKFKEAVHWIIYLAKYYKCNVNLIKPFLTDDGKKKQLANNIYFTKKMLDGVNIVYGIK
                     TAKKKGTFEEEVFRFARTIEADLVLIMSDKYDDFLDSKQEAKHDIPVMCVNPRIRKYQ
                     SFY"
     gene            30808..33588
                     /locus_tag="PLW22_00750"
     CDS             30808..33588
                     /locus_tag="PLW22_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU73257.1"
                     /translation="MKLSLKEKIIFLFTGTSFLILFFLLFLNSARFNRLSIETAKSRI
                     SLLADKEVKKFEATLERDMSVARTIASSMAVTIKEGKTSNIQEYNNLYTNILEQYPDV
                     LSVWDSWELKFVEPGYTYEHGRIKNYAWREGGKILANTIRTEKTESSAEYITLKTNKS
                     ESLEEPYFDNYEGQTENILMTSVIAPCIIDGNFAGVAGLDIPLSNYHQVVKSIVPFKD
                     SYAFIISPQLKFIAHPDKDLIGDDALFSYESIFTSHGVQDKVINGEPIEFEATDINGK
                     LSFFTFRPISVGKCPQHWSFVLITPRETILAEARHVFWTTLTIGLIGILLLSGFIYLM
                     TLKFLINPINKVTQNLERLSKGHVDNSMIVDVDNNDEIGTMITHLNKTVEGLYKKTEF
                     AKQVGMGNYDTSLELLSDEDVLGKSLIEMNENLRKAREEALIRQRDEEKRRWVNEGLA
                     RFGDILRQNNNDIEALSASIVKNLVGILNANQGGLFIYNDENTDEPYFELTAAYAYNR
                     QKFIKKHILLGEGLVGTCALEKQTIYLKEIPENYIAISSGLGEAKPRNLLIVPLKVED
                     KIFGVLELASFNEFEDYQIEFVEKVAQSIGQTLQSVRTNIRTAELLAKTQQQAEEMKA
                     QEEEVRQNLEELETIKEELEKRTAEMEENQKQLEWEKSLLDSLLNYLPDKIYFKDLKS
                     RFIKVSKSTLEFFGMSKQEELIGKSDFDFFTEEHARPAYEDEQRIIRTDTPIIGIVEK
                     EVMNDGRVTWAETSKLPLKNSHGETIGTFGITRDITASKMMEEEISKKSEKEEAFQRD
                     LEKKVIELENLYKAINDSAYVIEYSPEGNVTFINEAYLQLLNLNADDVIGKHHSYQME
                     FTDEQRKNYKQFWDDLNNGIVRKETSKFSINDKTYLFYETYTPIRDAEGNVVKIMKIA
                     FNVSHLLSENQI"
     gene            33624..34937
                     /locus_tag="PLW22_00755"
     CDS             33624..34937
                     /locus_tag="PLW22_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOU73258.1"
                     /translation="MLSWKKLLSPIRTGESPSKSTIDYTRTNFQRDYDRLIFSSPFRR
                     LQNKTQVFPLPGSVFVHNRLTHSLEVSSVGRSLGRSILNLLSPERFECPAQLITDIPT
                     IVSAACLAHDLGNPPFGHSGEDAFRHFFSNHHIPELDSWEASDLLRFEGNANALRLLT
                     HQFRGRRTGGFRLSYATLASIVKYPYPSTLKEKKFGYFKSEADVFNEIASQTGLIQIS
                     NGRYCRHPLVYLVEAADDICYQVMDIEDAHKLGILSTSTVTNLYLSFFDDETTSKKKD
                     IHETLSSVNDINERIAYLRAITISKLIEQCSSNFSKSYDQIMSGQPVKPLIDQMEGAS
                     AKAMDEIRKVSVNQVYNHSKVVEIEIAGFTIIGELLDIFTSAVLEPTKPISKKALRLI
                     PEQYLAESSDNYSKVMAVVDYISGMTDIYALETYQLFKGIKLPSL"
     gene            complement(34940..35902)
                     /locus_tag="PLW22_00760"
     CDS             complement(34940..35902)
                     /locus_tag="PLW22_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU73259.1"
                     /translation="MLNQIKEPISNHLNDFEPFFRKNMGSDIKLLDLITNYIVRRKGK
                     QLRPMLVFLSAGACGQINQSTYVAAGMIELLHTATLVHDDVVDEAYERRGFFSINALW
                     RSKVAVLVGDFILSRGLLVAIENGQVEMLRVISSAVKEMSEGELLQIERSRKMNIDEE
                     TYYDIIGKKTASLIASCCANGAISANATKDMVDRMYQFGKYLGMAFQIRDDLFDYQPS
                     GLIGKPTGNDIKEHKLTLPLIYALSQSDTHERRRIIRLINSGKKANIAEIVDFAKDKG
                     GIDYASKKMFEFRDNAKQTLEIFPDSEYKKSLLLLADYMVSREK"
     gene            36061..36360
                     /locus_tag="PLW22_00765"
     CDS             36061..36360
                     /locus_tag="PLW22_00765"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU73260.1"
                     /translation="MVQVEELKIEQLEQAASMLKAIAHPLRIGIISLLEKSGQLTVTQ
                     IYETLGIEQSTASHHLGILKDRGVLSCKREGKNTVYFLRNENLGKILDCVGRCTV"
     gene            36371..36880
                     /locus_tag="PLW22_00770"
     CDS             36371..36880
                     /locus_tag="PLW22_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOU73261.1"
                     /translation="MTTFDIIIVILLLVGAVRGFISGLIMQLTSLAALLLGIWVAIRF
                     SDFTAMLLMEKAGLTGQYIPLVSFAITFVVVVIAVHFTGKLIDKFFNLTPLGIVNKIL
                     GLVFGVLKYALIISVIIVFVEKANQRFNFYSEQTKNKSMLFKPLSKLAPSIFPYLHFE
                     SISERLKAK"
     gene            36985..38280
                     /gene="tyrS"
                     /locus_tag="PLW22_00775"
     CDS             36985..38280
                     /gene="tyrS"
                     /locus_tag="PLW22_00775"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOU73262.1"
                     /translation="MNFVEELKWRGMVHDMMPGTEEQLTKEMTAGYIGFDPTAESLHV
                     GSFAQILLLKRFQLAGHKPIALVGGATGMIGDPSGKSQERNLLDEETLQRNLEGIKRQ
                     LSKFLDFESGKPNSAVLVNNYDWMKGYDFLSFIRDVGKHITVNYMMSKDSVKKRLGEE
                     SKEGMSFTEFSYQLVQGYDFLWLYQNMNCKLQMGGSDQWGNITTGTELIRRKLSGEAF
                     ALTIPLITKADGTKFGKTESGNVWLDARLTSPYKFYQFWLNTSDEDAERYIKIFTLLD
                     KTTIDSLIAEHKKEPHLRKLQKTLAREVTVMVHSEDDYNKAVEASEMLFGNATTDMLN
                     RMDEDTFLSVFEGVPTFEVKKSELAKGIPFSVLAAEITKVFPSKGELRRLVQGGGLSI
                     NKQKVDNPDITIGTETLLNGKYILVQKGKKNYSIILAVD"
     gene            38398..39402
                     /locus_tag="PLW22_00780"
     CDS             38398..39402
                     /locus_tag="PLW22_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOU73263.1"
                     /translation="MDNNDISMDKSMLSIAQTIWNGRKTIITVGIIAIIISTFASLLV
                     KNQYKATATIFPPVANQVSKELITENKQEGLTTFGETEEMEQFLQVLSSRTLKDKVIS
                     RLNLQQHWGIDSTSKSYRFRSYQKFDEQVKMKPTRYQSINIEVMDRDPEWAAKIANTV
                     VEYSDSLMRAIKAQVAVNALMALEKQFALVEHDLNNLQDSLALAISNGVVDPKRQATI
                     FYKEYINALLKANNAHLIKLKSEMYRFSDHGAKSIRYAAEIKDLAERLNELRKSIVVA
                     RIEANQEIPTQFIIDKADIPDKKAYPKRSIIVVTSTLAAIIFAIFLLFLQEYLKRLKI
                     "
     gene            39406..40935
                     /locus_tag="PLW22_00785"
     CDS             39406..40935
                     /locus_tag="PLW22_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU73264.1"
                     /translation="MIPFAGSVYPLMDIIKSILSHIKNKWIIVSSIIFILINAILIGT
                     ERDFFVILPIIFIFLLAYLSFPLKFYLAYLFISPLSIPLSEFFQEISIDLWLPSELML
                     VALAPIALIMLLSNNHQNRWFTWHPVLLTIYFFLGWIALTSITSSMPLVSLKYLILKT
                     LYFIVLLYLPFILLVKGNITFKRFIAFYSIGLFVVILLTLSKQWEYGLFNKFVAHGSC
                     NPFFTDHTSYAAVLALLIPVSIALLISSNTATRRILFLALTIVLIVAFVLSYTRAAWL
                     SLMVTGVVWVAWLLKIRFRYLALLTIIFFGSIFYFQDNILIWLNQHKTASSGDIRKHI
                     SSIANIRTDESNVERLNRWTSAIRMFEEKPLLGWGPGTYMFQYAPYQASYLKTRESSN
                     LGLKGNAHSEYLGLLSEAGLPAALAFILIIGILFYKGFNLALKLKPKSADRVILIGVL
                     HGLITYDVHGFMNNFLDMDKVAVLFWGYAALILAMEHLYSKSNPRIEISSKTSASDSL
                     S"
     gene            complement(40869..42026)
                     /locus_tag="PLW22_00790"
     CDS             complement(40869..42026)
                     /locus_tag="PLW22_00790"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU73265.1"
                     /translation="MAQAISAELKTEELIDRFKRILNDDLGIDRILLYKIEEGKWQLL
                     LNSNCPERVVENINVERDLLHFNEITFVGVSENPILLELDVIIPVIQNNQPVGYVLIG
                     DTNEYEKGVSATIRHLNFVQTLSIIIFVAIENLRLFHKSLEQEAMRKELELASRMQSM
                     LIPSPADMPKFNGVSIGSYYNPHFEVGGDYYDVIALSPNDLGFCIADVSGKGISAALL
                     MSNFQANLRALFSKDISLSALVEKLNERVMATAKGEKFITMFIGRYNNQTRKLEYVNA
                     GHNPPFMFVRNSSKIIQLSSGCVGLGMIDDIPIINQGVLNIDEPSTLLCYTDGLVETI
                     DGDKVVYNTRILEKNFSRNHSVQQIINTIAQERESDADVFDDISILGFDFE"
     gene            complement(42190..42756)
                     /gene="efp"
                     /locus_tag="PLW22_00795"
     CDS             complement(42190..42756)
                     /gene="efp"
                     /locus_tag="PLW22_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOU73266.1"
                     /translation="MATTADFKNGLFILFNGKIYTIVQFQHVKPGKGGAFVRTKLKSL
                     ENGKVIENTFNAGVKIDVVRVERRPYQFLYKDELGYHFMHQETFEQISLDEYKIENPD
                     LLKEGQYVEMMVEADNENILTCELPPFVEMEVTYTEPGLKGDTASSTALKPATVETGA
                     VINVPLFINIGDKIKIDTRTREYSERVK"
     gene            complement(42879..43619)
                     /locus_tag="PLW22_00800"
     CDS             complement(42879..43619)
                     /locus_tag="PLW22_00800"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOU73267.1"
                     /translation="MNAHAQNGKSAFIGGEKLKYQIYYGFINGGEATLQVNEQVFNGK
                     PAYHLYLNGRTVGIANTLYNVNDTYESYTDPATHWPYFSIRNIHEGRYRHYSTQTWDH
                     WSRPDSSIVISSKTGKVVVVKGCQDILSSVYYLRNKMLTIPPLKQDQTITVDTYFTDE
                     KYPLTVRFKGYETIKTKFGLVKCMKFMPVVITGRVFKTKDDMTIWFSNDSNFIPIRIK
                     FDIFVGSVYCDLIEYKNLANEFTALEKK"
     gene            complement(43654..44358)
                     /gene="tsaB"
                     /locus_tag="PLW22_00805"
     CDS             complement(43654..44358)
                     /gene="tsaB"
                     /locus_tag="PLW22_00805"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOU73268.1"
                     /translation="MAKILCVETGTSVCSVAIGDENGILGVRELSDPKAHSTMLPRLI
                     NDVLTDASLTPSQISAVAVSKGPGSYTGLRIGVSMAKGICYTSGIPLIGVCSLQAMAV
                     GLSLSHPNIPADAILCPMIDARRMEVYSALYNPQGEPVSDVRAVVVDENSFAEFLQNQ
                     KIIFFGNGSAKCKDVIKSKNAIFIDGFEPSARFMLPIALNAYKNEEFENTAYFEPFYL
                     KDFVATTPKNKIIPNS"
     gene            complement(44372..45676)
                     /locus_tag="PLW22_00810"
     CDS             complement(44372..45676)
                     /locus_tag="PLW22_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU73269.1"
                     /translation="MKKGLIKYLLGAVVILIVFAVVAKKKGWIGKPLTTPVYVEKPEY
                     RTITEAITANGKIQPEVEVKISSEVSGEIIELPVKEGNWVEKGTLLCRIKPDTYISLK
                     ERAIAAVNSANARLIQAKAQLAQSELSFNRSKQLYEQKAISESEFETARTNYEVAQAE
                     LKAANYSVESAQASLREAEENLRKTTIYAPISGTVSKLNVELGERVVGTIQMAGTEIM
                     RIANLNRMEARVNVNENDIVKVKIADTALIEVDAYLGRKFKGIVTQIANTANVQGITT
                     DQVTNFEVRIFILEKSYKDLLTNSQTSPFRPGMSTTVEILTNTKENVLTIPILAVTTR
                     IDTVKKTGEWEKVENNTQPVESKPTKVEKPKEIVFITKGDTARIVEVKTGIQDNQYIE
                     IIEGLKGDEEVITAPYSAISRKLKDKSLIRKVKNKDELFKTE"
     gene            complement(45767..47107)
                     /locus_tag="PLW22_00815"
     CDS             complement(45767..47107)
                     /locus_tag="PLW22_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU73270.1"
                     /translation="MKKTILPIFILLILTSVVFSQEPWTLERCIEYALANNIQVKQQE
                     LNVKVNEVNLLQSKMDALPTININGNHSYSFGLVTNYLTNTKEALNTQATSFSVSTGL
                     PIYNGFQLTNTRKQNHFDLKASVADVEKVKNNIALTIASLYLQILYQQELVEIAKRQV
                     ELSTMQLERTKVLVSAGSLPEGNLYEVEALVASDELQLVNAQNQLNLSYLNLTQLLEL
                     KTTEGFQIAKPVIHQLDSVKLVSESPSQIFGVAEGIMPQIQSARYRLQSSELGLKIAK
                     GGLQPRLSLGANYNSGAQYRIQDNYAFDGDPFMTQIKNNASKSVGLSLSIPIFNGLSA
                     RNRVTTAKIGLMNAQLALESERNALYKDIQQAYADAIAAEKKLQSSQKSKTASEESFR
                     YTQNRFNLGLVTALDFTSAKNKLAQAETDLLQAKYELIFKTKILEFYKGLPLKL"
     gene            complement(47276..48025)
                     /locus_tag="PLW22_00820"
     CDS             complement(47276..48025)
                     /locus_tag="PLW22_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU73271.1"
                     /translation="MILLVISDLHIDTGKKLGTFGWKAKAFMSTLDRVIEHYGVEKVI
                     LNGDIFDLYKYSYAEIAEVNAKIIDYFNRKGFVQLRGNHDIWVPHTHDHFHIKNSEGK
                     TIHIEHGHKADFLNGTRFGRFISVTMYSFLKWLVRFRWVERKFYSAVDYFDDVNRIPR
                     KYNTIKYLMYALKLLKKYDMVILGHTHKLESHKVYYLNSKKIYLNCGTCSLGRFQAVV
                     VDTESFDFETIKIPRKTEFDESQLPIFKRKR"
     gene            complement(48060..48587)
                     /locus_tag="PLW22_00825"
     CDS             complement(48060..48587)
                     /locus_tag="PLW22_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73272.1"
                     /translation="MKRFFSYYAVVAFLIYVLAILVVKLIIKGYQEFSISFSGILIES
                     AIAGFVFVFLFFGPIWLNLSKTLGYIESDSFEEPAYGHRLSEQVETPIREFSELKERF
                     TSSYSYVKVSEDRAAIKFHNGLTWKNWGAGGIIFFNAENRNVRIVLIPFIGYTMHATK
                     QLEKEMKRVKEVLMG"
     gene            48905..49417
                     /locus_tag="PLW22_00830"
     CDS             48905..49417
                     /locus_tag="PLW22_00830"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOU73273.1"
                     /translation="MKESKDLVRWYAVYTKPRNEKKVYARLVEKGIETFLPLQKKLKQ
                     WSDRKKLVDEPLFRSYIFVHIDLKRYYDVLNTQGVVRYITFEGKAVPIPDRQIDQIKQ
                     LLVQDIELEAVEGQIEPGTRVEVRFGSLQGIEGEMVEHSGKKKVVIRIDHISHSLLVT
                     LPAEYVVVKR"
     gene            complement(49459..50166)
                     /gene="pepE"
                     /locus_tag="PLW22_00835"
     CDS             complement(49459..50166)
                     /gene="pepE"
                     /locus_tag="PLW22_00835"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOU73274.1"
                     /translation="MVRLLLISNSTNAGEAYLEHPKKEIEKFLGSIKRVLFIPWAAVT
                     FSYNEYEQKVQERFSEFGVEVDSIHHHRNAKLAVQEAEAIVVGGGNTFKLLKTIQQTD
                     IIEAVRNKVLSGTPYIGWSAGANVACPTICTTNDMPITEPDSFSAFSLVRFQINPHYL
                     DANPEGHAGETREQRIMEFVEMDPHVYVVGLREGTMIKLEDVQFSLIGPKPARIFKKG
                     QPPREVYPGDDLDFLFE"
     gene            complement(50217..52148)
                     /locus_tag="PLW22_00840"
     CDS             complement(50217..52148)
                     /locus_tag="PLW22_00840"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73275.1"
                     /translation="MLRISIISAILTFAILFNNGLAQNHSIDSLNQALQKQESDLNRY
                     KTLTELAIAYSDSNYEKSLDYWKSALSLANRLGKRELVANAYHQIGFSYMKMGEFRLS
                     LENLENAGTIYKYLDSVRLLAGIQNDIGLIYRNWGKYDKALEFYLNALDLYRKIGDIE
                     GTGIASNSIGQIHFYRENYPKAIEFFKEYLEINEAKGNSRAVAGASNNIASAYLELGK
                     YDDALEYFLKSLHIYDSLGISIGVAIIQDNIGSLYFKQNLLDNALLYHQNALEMFQSL
                     KSVTRVCYTQKNMGQVLLAQGKVERAINMFQESLNKAKQIGLKDVESDCNKFLSECYV
                     KTNNFQKAYSYLLDHITIKDSILNSETLQKIEELQAQYERDKKEQEINAMNSKLKAQR
                     LNLFIAMGFSVVFISLFLLVVIENRKKKRALVNLELLRNHLFQNISQNLCNLQMVKNS
                     YEVEGYFSAWWEAKIKKVDEKPNFFHFLYKNYTFCYYVIAAEPNACCDFINFNIYNIV
                     IEYIKNNGELNEGVLEPLNLYLKDDPIVKSLGKNSIKLYPFILYKKQILTLCPDNMAF
                     RQFGSFIVSSTLNWSNLRPGDIVYLFGTSDDKSAMNELRKVVKSIDLMEFAEQKDLAL
                     NFIKSLELKEDAFIAAFKV"
BASE COUNT        15059 a        11562 c        10703 g        15020 t
ORIGIN      
        1 ttggaataat tattgcaacc attgaattga tatattcgtt ttactacatt gcgggattat
       61 ttaaaatttt gatctgatga aaaaaatttt agcttttatc tttctttcat tttccctaac
      121 agcaaattcg cagccaaaaa aaattaagct gcaagccgat gatattcaaa tcggtaagga
      181 ctaccatgaa aaattttatg ccacttcgca aaaaagttat cggttaaagt taaaaattga
      241 tgccctggag tgggataaac agcaagctaa gacaggggaa acctttacaa agctctggtt
      301 caaaaattca ttgcccgatg gtaacattgg tgagcccgaa ataccagttg taaagaaatt
      361 gatttgtatt ccgcttggcg cttcagtttc ggcaagggta agcaaattca agatggacga
      421 agttcaactt tcagccaaag gtattcaata cccactattg ccggtacagc catcggcacg
      481 caaggatcag gatacgcttc aattaccatt tcaccttaag cccgaagctt atcgcaagtc
      541 aaccttccaa agtgtaaagc ccgaggtttt ggttgaggtt atggggaacc tcaggggata
      601 caccattgcc cggttaaccg ttcggccggt tgagtataac ccagctcatc aaaaaatcag
      661 ggttttcagc gatgttgagg ttgatgttga tattcaagaa gccgatacaa aaagcgcttc
      721 aaaaaccaac ccctactatt cgccatactt cgacgttgta tataaatcga tgctaaatgc
      781 aggcggagca acctacgatg aacaccccga tttaacgcga tacccggtaa gcatgcttat
      841 tgtatcgcac cgaatgtttg aaacagcatt gcaacccttt gtggagtgga aaacccaaaa
      901 gggttttagc gtaaccgtga agtacaccga tgagattgga gcaacctccg atgccattaa
      961 aacctacata cagggtgttt ataactcggc tacccctgaa aatccggcac caactttttt
     1021 ggtaattgtg ggcgatgtgg agcaggttcc tgcttcggca accggctcac aatcgggcaa
     1081 gctaaccgac ctttactacg ccagtgttga tggcgatatg ttcccggaga tgtactacgg
     1141 acgcctgtcg gccaccagca aggctgaact tacggccatt atcaacaaga ttctttacta
     1201 cgaaaggttc cagtttgccg atgcttcata cctgaacaat tccactttga tagcaggtgc
     1261 cgatgcatcc tggaacccgg caatagcaca gcccaccata aagtacgcaa ctgcaaccca
     1321 tttcaaaccc tcaaatggat ggagtaatat ttacgagtat gggattacaa atgatccaaa
     1381 taatccctct gcatcatcgg gctatacagg gtgttacgat cctgaaagga ttgccgtggg
     1441 gttcataaac tacactgctc actgtaacga aaccgactgg caggatccag cgcttaccat
     1501 aagcgcagta gaggcattca tcaacaacca gcaatacccg tttgttattg caaattgttg
     1561 cctgtcgggt aattttggtc actctgagtc ggttggcgag gcatggctcc gtaaagcgaa
     1621 tggtggcgct gtaacctaca ttggttcatc acccaacagc tactggaagg aggatatgta
     1681 ctgggcggtt ggtgctttcc ccatgagcgg cgataacaat gggtacgttc ccagctttga
     1741 ggaatccact accggtggat acgatgctcc ttttgtgagc gcatacacca ccgctggagc
     1801 gataatgttt tgcggtaacc ttgccgttac ccaagctgaa ctaaacgact actcccgcca
     1861 aatcaactcg acctactact gggaagccta caacattctt ggcgatccat cccttatgcc
     1921 ttacttcaaa atccccgagg caaactcgat aacgttccct gcaaccactg ccgttggtgt
     1981 aagttctgta agcattggag ccaaggcaca aacctatgta tcgttgtcca gaaatggcca
     2041 aatcattgat accaaatact atccaaccga cgacttgctc gattttgaca tacctgggca
     2101 agctgaaccc ggaaaaatag ttttaaccgc cacccgaccc caaactcaac cctttataga
     2161 taccattcat gtttttgttg ccgaaaacgc ctacttaacc ctcgcatcga ccacaattga
     2221 tgattccgaa gggaataaca atgccactgc cgattacggt gagaccatca aggtaaacct
     2281 actggtcaag aacgtgggca aaacaaatgc aacaagtatt ggtgcaaaaa ttaccgcaag
     2341 cactggacta attgagctga cttcagccga ctcaataggc attggaaata ttgatgccga
     2401 atcggaaaaa tgggtaaatt cggcctttac cttcagcatc gcaaccaata tcgacgataa
     2461 gtactcacag gttttcccaa taatctttac atcaaccgag agatcgtgga catcgaattt
     2521 aagaattaca gcagccgcac caaaaatggt tttcaacgga tacactgtag ttgacaccct
     2581 aatgggcaac tcaaattccc agtttgaccg aaacgaagtg gttgacctaa aatttgaatt
     2641 tcagaacaaa ggcggttcag ccattcccga tttctcggca caggcaacca ttcccgattc
     2701 actcctcaac gcggtaagtg tcaactttga acccatctca aaccgaacca ttcagcctaa
     2761 cgaaaaagca attcttaccc tgcgggtaag cacatcacca aacctaatcc acgatacaat
     2821 ccccattgac atagactata gctccgttag ctacagcaat gcctcggatc agtttaccag
     2881 caaggtacca gtcttacttt ttggaactac caaaatgact aatggtaccg ttaaaacctg
     2941 tgggactatt tttaccgaca ccggtggcga gaccgccaac tacaataacg gcgagaacta
     3001 tacactaact tttgaaaccc ttagcgatat tcagcgatat agtgttgagt ttatatcgtt
     3061 caaaaccgaa ttgggatacg attacctcca catttacgat ggggctggta taacctcaaa
     3121 tgccattact ggcagcccgt ttaatggcaa taccggcccc gctccctttt ttacaagcgg
     3181 aaacagtgtt acattccgtt tcacatccga tggctcaaca acggcctcag ggtggaaggc
     3241 aaagctagag tgtattactc cacaacaatt gcccaagtgt attgaaaacc caaaacccgg
     3301 gaataatgag cttaatgttg aatataatcg gttatcgtgg aggaccacct ccgatgccct
     3361 tttctacgat gtttacatag gaaccaaccc cgattcttta gttctactaa aaagggttac
     3421 cgaggcatat gttgatattc cgcttatgcc gcaaactaat tactactggc gtgtaatgcc
     3481 cggcaaccat ttgggtttat gtgataaacc ctgcgaggta tggaatttta ctacagcttc
     3541 agtaattgga caggttttaa tgaccaactc taccgttgaa gtggacagcc tatggttcta
     3601 cgactctggc ggacctacct caaattatgg taataacgag aattacacaa ttaccttcaa
     3661 gccaagaaat aacggtcaaa aggtaaaggt tcaattcatg gagtttgatg tggagtcgca
     3721 ctcaacctgt agctatgatt atatgattgt gtatgatggt cccagcacac aaagtcctga
     3781 acttggtaaa tactgtgggg ataatatgcc accagcatat acttcgacga gcgcaaacgg
     3841 cgagctgaca ttcaagtttg tctccgattt gaacacggtt ggaacaggat ggaaggcaaa
     3901 gattactact acaggtagta ttaccactta tccagtaact ttcaatgtta aaaacaatgg
     3961 tagtgcaata ccttgcgcag cagtaagtac tgcagatgga attaaattta catcgagttc
     4021 gggttcggtt aatttcagtc tacctaacgg aacatacagc tacacagtaa gagccaatgg
     4081 ttatgaacca gttttgggtt ccacccaaat tgccggctcc gctaaaacaa ttgatgttaa
     4141 tttgaccaaa cacgatacca ttcaactact ggtgcgttct gcccttgacc ttgccgccat
     4201 accgctagcc aaaatactgg ttaacaacca aacctactac acatcgaacc ttgggatggc
     4261 aaccatttat atccctgctg gggattatac gtttaacctc accgctgatg gttactatgc
     4321 aaaggataca atcataacgg ttcaagcagg tggtggtaac cacaatatta accttttgcc
     4381 ggttagctac aatgtacagt ttactatctc cgatataaac gggaacaaaa ttcctaatgc
     4441 caaggtattt gttgataccc ttttaggcga aaccaatagc gaaggattag cctcattcac
     4501 cgtaagcaga ggattaaaaa ccgtaagcat tcaaaaggag ggttttgtta gtaccgattt
     4561 ttggatggaa ctaagttccg atagctctgt aaatgttttc ctgacccccg tttacggcga
     4621 tgttcatcag gttatgttta gtataacagg aaccgggcca aaaggaacat ggccgctgga
     4681 taacgccttt gttaaaatct ataacggtac ccagctttac catcaaggta aaacacataa
     4741 tggtgttgca acactttacc tgcccaatgg aaactactca tacgaggtga gcctggaagg
     4801 gtatcaaggc actgaacaga ctgaattcac agtaaacagc aaccccatcg aaattccagt
     4861 tctgctaaac cagctaacct atagcatcac ctttgatgtg caaagcgggg gtagccctgt
     4921 tgccaatgca accgtaaaaa ttaacggcta ttccgaccag cttaccgacg ccaatggctt
     4981 agctaccttt aaccaagtag gatacgaaaa ggggttacag tttagcgtat tacaccccaa
     5041 ctactacacc atttccggaa ccttagatgc tacaaagtca gaaaccataa gcgtaaacct
     5101 caccccaacc tcaaccgata ttgttgaccc cgacaagaac ttatcaatgt accccaatcc
     5161 ggcaacaagc cacatcagga ttgaggcaaa tgagacaatt gaatccgtat ccatagtttc
     5221 aataaacgga acattaatgc attgggaaca agtcaacaat agcaggcaca ccatcaacct
     5281 aaacttgcca acaggagttt acgtggtaag gtttacaatg agtaacggta ggttatacta
     5341 tagaaagcta atggtacact aaccatatca accaacattg ttaataagta tgggcataaa
     5401 aacatgccca tttcttttta tatgaccgaa atatttttac atttggcaca aatcaatgtg
     5461 tttagtaagt gttaaggttt ttcaggtttt tctcgttaca caaatttgct ctggcattat
     5521 cattggttgc cataaccacg ctatcggttg tggtttggca attagcccaa aatatcgacc
     5581 ggcagaacat tcaaacaatc agtgccgacc ttcggtcagc aaacaacgat gtaatcaggg
     5641 atattgagcg tagtttctgg gacattaagt tagccgatta ccaaaacgac gatgataacg
     5701 aaattatttt tgggaagaac attaacctgg aggaatcatc ccttttcaac ctggacaata
     5761 acaagctttt tggcaccaat gtgcttttac tccataacaa caggttttac gctaaggcgc
     5821 ttttcaaaac tagtacccac gaatttgagt actggtatcc aattgctaga acttttaacc
     5881 tttacaacgc cttaaccggg aaccggttag caattctaac tgataatgaa aataccctcc
     5941 ttagccagcc attaaccctt gcccgagcaa aaaacctaac aggcgataaa acggctttaa
     6001 tagcagccga aggagttaaa agcaatagct gggttttact tgttttacca ttgctgctgt
     6061 tggttgtggc tacagctgta atttactcac tattaaaaag gtcgaactgt cgcttttcag
     6121 gtactaattc ctccggtaaa acagtacata ataatgcccg cgaagtgcta gcgcaaatta
     6181 acctaaaatc ggcacttaac aactcaaacg ttgcgcttag ggttattgat accaacttta
     6241 gggtgatatt ccagaaccag gagtacgaaa agctaacctc ggttacgctc agtaaaccaa
     6301 accatccatt atgccacgaa tcgttcggaa gcatatattg tcacactgac gagtgccccg
     6361 tcaggagtat cctaaatgga gccaaagagg ttaaacgcga ggagacaaga ttcctaccta
     6421 caggcaaaaa aattctagta gagctgcatg cctacccgtt aaaggatgat tatggcaaca
     6481 ccgttgcggt tgccgaggag attaaagata tcaccggagt tcactatact gaggaaatac
     6541 tccgacaaac cgagaatcag tttaccgtat ttatcgactc cttacccttt ggggtattca
     6601 ttgaggatgg acttacccac gaaattgttt accaaaactt ttacctcaag cagcttacct
     6661 gtaataccgg tttcaaggag ttcatcaagg gggctgagat aattggcgtg gatgccgata
     6721 acaatgttac tgagttccag ctcatggact cctccggaat aatgcggtat ttcaatctcc
     6781 atcagttcaa attccttggc gtaaacaaca gtttaaagat aggtggactg tttgttgata
     6841 atacccgcaa aaaagagatt gagcattacc gcgatgtgct atccaaggca attgagttta
     6901 ctccggttag tattgccatc ctatcgccct actttgagtt tgagttcatc aatccgaact
     6961 ttactgagat taccggctta accatagaga acctttttag caaaagcatt tccgatatca
     7021 acctggaata taatggcggc aagcatattc aaaaggcact cgactccgtt agggctggta
     7081 aaacctggca gggtgagcta cagctaaatg gcaagaatag ggagcggatt tgggtaagcg
     7141 cctcgtttac cccggtaatg tttgaatcga aacttcagca tatccttgta atacttgaga
     7201 atattactcg ccgtaaggaa tacgaaaagg aacttatact tgccaaaaac aaggctgagg
     7261 aatcggatag gattaaaacc acattcctga acaacatctc gcacgagatc agaacgccgc
     7321 ttaacgccat tattggattc tcatcggttt taactgaccc atacctgggt caatttgagc
     7381 gtagcggctt ttccgattta atttacaaga actcacagga actacttcaa atcattgaga
     7441 acctgctgga aatatcgcag attgaagctg gcgaaatcaa gataagcaag accgagttct
     7501 ccgttaatgc cctgatgaac gagatataca gcacgtttga ggagcaggaa agaggaagct
     7561 cgcgtattaa gctgagcctg cgtaaggaaa ttttccagga cgacctagtt gtgcttagcg
     7621 atccgtcccg aataaaacag gttttaaagc acctgctttc aaactcgttc aagtttacca
     7681 ccaatggttt tgttgagttt ggttacagac ttaaggatga gaataacctc ttattctacg
     7741 ttgttgatag cggcattggc attgaacccg acaaaatgga tttgattttt aatcccttcc
     7801 gccaggccga tagctcaagc acccgcaggc atggtgggct gggtttaggc cttgccatat
     7861 ccaagcatgt ggtggaaaag atgggcggga agatatggat taacagcacg cccggctcgg
     7921 gaacaagcgt attcttctcc attcccttta tcccggttaa acttaagttt gaaaacgcaa
     7981 atactggaaa cgggttaaag gagtttaact gggacggcaa aaccattctg atagccgacg
     8041 atattgatgc caactttgta ttctttaagg cagccctaca gaaaacaaaa gccaacctga
     8101 tttgggcgcg taacggcctt gaggccctaa attacgttcg caatggcgag cccgttaacc
     8161 taatactgat ggacctggta atgcccgaga tggatggctt tgaggcaacc cgtatcataa
     8221 aatcaataaa ccaaaaacta ccggttgtag gccaaaccgc ctaccctgaa accatatcga
     8281 gcaataagct tgccgagtgt ggctttgata ccgttctgga aaaacccata cgcaccaacc
     8341 aaatgcttat ggaaattgat aggtttttgg tgaactaata gtgatggggc ggaatgattc
     8401 ggaatggtag aatgaaagtg aattagagca cgttaaaccg aaatggggat acgaaacatt
     8461 tttagtgaaa attcatcaat tgacgaatct cagttcaata ttatcataag taataagttg
     8521 cattgattgt ctaaatctac cttgctttca ttacggcttt aaactacctg gatcccaaaa
     8581 cattaaattt cgtttcgttc tattttggaa ttccccctga atcattcaaa tgctaaacac
     8641 gaaaacagtt gagtccccaa cgctggaact cataaaatca cttctggcta agccctatct
     8701 aaaaaatttc taacttgtag gaggcaccgc cttggccctt atgtatgacc atcgtaggtc
     8761 gtttgacata gatttgttta caaataccga ttttgatgca tacaggttgt tcgaacaggt
     8821 gcagcatgat ttttcatttc aagtgaactt caccgctcag aatacaatta ggggtagtat
     8881 aaattcaatc aaagtggatt taattgccta caacccccat ttattcccaa tagtgcagca
     8941 ggaagggatt cgcttgctgg gcagggtcat acattgcacc cgataaacct acctccagcg
     9001 gaacgtaaca acctcgcgtc catccatttg gtaggcgaac tgcctgccgc tacacttcac
     9061 tgttacgcct gcaggctgac tggacgtatt caccgcgata agcacctttg agccatcggg
     9121 gttcaggaag gccacgttgg ataccgattg cccgttggat accagtcgca ccgctccggg
     9181 tgccacaaac ttgccgaaat gccccagggc gtagtactcg gcattccttt taacagctcc
     9241 gttggaggcc acctgcacaa ccccccagca gtttgagcat ccgttgtttt tggggccatc
     9301 gttttcatca agagcaagat tccacagcag aacggtttta ctccagcacc gcatagcttt
     9361 caccataagg gtttcggagt accaaagcag gttgtcgcca aagtttggtg cccatccccc
     9421 gcccgactgc tcggtgaagt aaatgttttt atcgggatga gcatcgtgaa ctgagcaaag
     9481 ggcttcaatt tcgcctttat acaggtgaaa acccgaccca tcaacatacc tgcgcgttac
     9541 gctatcgttc aggatgctaa tgggatactg cggggaatca cagttatggt cgaacagaat
     9601 tatcctggtt ttcaggtcat tctgtgccat ccggggtccc aggtagtcgc gtatgaactc
     9661 gttctgctcc tgcgcgctca tcagcatgca gggataggtg gctgtgccaa actctggctc
     9721 attttgaacg gttatcccgt aaatgggaat ccccagctcc tgcatggtct tgatgtactt
     9781 taccaggtag ccggcataaa cgccatagta ctgccttaga aggcttccgc ctaccatgct
     9841 gttggatgtt ttcatccacg caggtgggct ccagggcgag gcaataatgg taatgtcagg
     9901 gtttactgcc aatatttccc tcatcatggg tacaacctca cccatatcca tgtccaggct
     9961 gaagtttgaa agtgaggtgt cgggcgtttc gctgtagcta tggtttccct gcgaaaagtc
    10021 ggatgacccg atggttaacc taaccacgtt tatgcctata ccctcaggag taaacaactc
    10081 gcggaaaagc tgcagacggg cttcaggagt aaggtaattc ttaaaaacgt aggccgacga
    10141 ccaggtaaga gcggcaccta cgccattaac cttctggcac agctggttgg tatcaacatc
    10201 aataacctca cctacttggt cgtttgaaaa gttaatgccg ggttgctgct ccagaagcat
    10261 ggatgagtcg gcttttgaaa cccatacctc aaccctatct tttggggagc tacatgccat
    10321 catggcaatt gcaaccagca tgatggtaaa attcgagtgt agtttcatga tactaatttt
    10381 ttagtaagtt aaacggtaaa aaaataaaga taattcttta aatcagtaaa gcaggacaaa
    10441 attttttcct ttattttcac ctgctacgat gaaaattcgt tcattttgcg gtgcaaagga
    10501 tcggagctgc tccctggaga ggctgatact tgcatcgcca atttttacct atcctataca
    10561 cccatggcta aagcccgggg ctatccagac ttcgcccctt cggggcttta aatacatccc
    10621 taaaagcaag cctttaatcc gccagtaggc ggacaagttc cgccagtagg cggacaggtt
    10681 ccgccagtaa ccggacaagt tttgaatctg atctttattc tttatctttt aaccttaaac
    10741 cttgaacctt tatcctttaa cctttaacct ttacttcacc ttacctccac atgaaagtaa
    10801 cgagcgattc gggctggacg gtaaaggtga acttattgtt tccacacctt acggcaagct
    10861 gtttcccggt gctggaaaaa ttcatggcaa ctacaacctt tgaaccatcg ggattttgag
    10921 cagcaacata cctgaagtct gccagctgag acacatcacc aacagctgtt gtacccacca
    10981 tgtaggcatt aggccttatt gcctttgaaa aatgggccaa agcgtagtac tcgttgaacc
    11041 gagtaagact tccatcggag ttaagggtaa taaccccacg gcagttggag cacccgccct
    11101 gaataaatgg cccccagtgg tcgtcgagca tcagattcca aagaagcacg ttctttgaaa
    11161 agcacgaaag ggtacccatg aacacatttt caacggtcca cttcaggttg tcgccaaaat
    11221 ttgttgccca tccgccgccc gagatctcgg taaagtataa acctttttcg ggaaactggt
    11281 tgtgaacggt gagcattgcg gagtaatcgc ccccataccc gtggaatgct gaaccggcaa
    11341 tgtatgggta agcaacagga tcggaaagaa tgcttatggg gtagtcgggc atatcccagt
    11401 tatggtccca aataagtatt ttggtcgaaa taccctctgc agcaaattta ggcccaagaa
    11461 aattccggat aaaggtaagc tgctcctgcc aggtcatctt catgccgggt gtaccaaact
    11521 cccacatggg ctcgttttgg acactaatgg catcaatagg aataccctcg ccagccatag
    11581 ccctaatgta gcggacgaaa tagccggcat actcgtcgta gaagtcttgg ataaggcttc
    11641 cgccgctcca cgccgcgctt gttttcatcc aaatgggcgg agtccatggg gtagccatta
    11701 tttttaaatc aggattaatg gcaagaatct ccttaagaac aggaatcagg tcgcgccggt
    11761 cgataggatg cagggcaaag gaatcaatgg aggggtagtc gcagtaggtg taaaggctga
    11821 gggagaaatc ggaggagcca atgctcaggc gcaggtagct aagtccgatg ccctttaacg
    11881 gatcgaatag ctcggaaagc aggctcgccc gttggctgtt tgtcattcgc ctaaggttgt
    11941 atgcagacga gccagtaagg gctgcgccaa atccatcaac ctgctggtat cggttaagcg
    12001 tatcaatccg tatggtaaag ggtgccacat cgtctgaaaa ggaaaccgac tgctctttga
    12061 agcggtaagc ctggttacag gtggttaccc aaacccgaac atcgcttaat tgggtagtgt
    12121 cgtcgggagg attgtcaaca ctgtcagaat catccgagca gccggataga agggtagcag
    12181 caagaaacaa acatgccaac agcattccgg cagcactaga aaatggccta ggacaaggat
    12241 ttttcatcag ataattttat ttagccatca taaaattcaa ctccagagga taaattatac
    12301 ccaagttact cctaaaaagc ttgaaaagca aatagccaaa taagaattaa cctgccggat
    12361 tctgctaatc caaacaggtt aattcttcaa cataagcacg caaacctaaa actaacccta
    12421 gttaattttc tgcgcaatca gaaagtagtt ccccttgcct ccgctcatgt caatggttat
    12481 tctgtacagc ccttcggtta gctcggttgt atgaacaaaa tttccattgg tattggaagt
    12541 caggttgttg gaaatgtagc cccaatccat tgaggagaac tcattatccc agctcttcga
    12601 ggggaaaagc ttaaactccg cttgcagcca ccatgatccc tcggtattca tcatgtacac
    12661 ggtagtctgg tatttgttct cgccaacttt ggggcagaag aagtaagcct ggcgagttgt
    12721 tacaccccaa tcatcccagc cccaccaaat atttgtaaca tcgttgataa aggtgggcat
    12781 ggcaattccg gcaccagtga gccaaagggc atcggggtaa acagcattat cctgccgcag
    12841 ccacatgtag ttgatggttc cagcccagta aacaacctgg tactttccgc tgggtgcctt
    12901 aaacgtaaga acaccgtcct taaaatcaaa aaagtcggga ttataggcag ctgccaggtt
    12961 agtaattccc tccacggtaa ccacctcgcc cttggtgaat tccacctcgc catggaagcg
    13021 cccatcctca cctataagca gggtggttgt attaaacttc acgctgggta gcatctcgcc
    13081 aataaacttc aactcaaaag tcatggtgtt gaaggatagc tgttcaacgg agtaggatgg
    13141 atcgctcagc ctgatatcgc tatcggtatg gctgccgata acaatcgtat tacccgagga
    13201 aacccaaacc cattcggcag ccatgagatt ttcggaggtt gcaattttac cgacaatctc
    13261 gttggggtat gaccccgaag ggctgaggta gaggaacggg ttgtcaggac ttttagccag
    13321 cttaatggtt tcgttggatg ttaccaaata aagggtatca ccaagtattg gtcgggcagc
    13381 atggagcgtt tcggtttgcg atgtagaaaa gccatcaatg ttgatggatt gaagcttcac
    13441 ggtaacctca ccattatcct cggcattggc agcaaagggc agcttaaact tagcggtaac
    13501 gttagcgttg ttacccttag tgcggatgga ctgtttatag atttcaacat cagccactat
    13561 cacctgaata tcaaaagttg aaagcggtgt tacctcatcg gaaacggtag cagaaacggc
    13621 aatggagtcg ccgtaggtta atccggtggt tgggatgatg gacagattac ttattgcagg
    13681 ttgagaagta gttttagttc cgtcgtcctc gcaggctgca agagcaacca tgactaaacc
    13741 tgcaatacat gcatacttaa aaatcttcat ggtatcgaat ttaaatgatt tagtaaccag
    13801 ggttttgcgt taacatgcta ttcaaatcac gctgttcctg aggaataggc caaagtagcc
    13861 tatatggttg tagttttccg gcatacagca gttgcccatt accccctcga acattctcca
    13921 tcaccgagat ggcccgtcct gttcgaagca aatcgaacca gcgaatcccc tcgaatgcca
    13981 actccaaacg acgttccttt tctatgatca accgcatttg ctcctttgtt gtgggggaaa
    14041 ccggaggaag gttaaccctg ctccgaatct ggtttaccag cgtatcggca ccaggaaggc
    14101 caagttcatt aagggcttcg gcctttagaa ggatgaaatc ggctaatctg aggagaatta
    14161 tgttggaggt accagcctca tctctgattt tataaacaaa tgggtaagta gctgcgggcc
    14221 agtttgcgtc tgtccagctg ccggtaacat cctcaaactt aattgaagag tttttgcgga
    14281 taacatcatt ttcggcatca aaagcagcca ccaggtcgtt ggatggagtg ttgaattttt
    14341 tccatccagt gccaagaaac atgctaacag cccagttgcc ggttccagta ctccagttac
    14401 catcgtactg gatttcgaaa atcgcctcgc tggagttttc atgctgaaaa tcccacaggt
    14461 agtcatagtt gcttaccaag ctgtatccgc cgttaattac cgcatcgcag tagtcgttca
    14521 cctttgtgta gtcaactggt tggcgggtag cataaaccct tgccaacaaa gcatttacag
    14581 cacctttggt aacatagccc ttgttggggg cagtaaccgg tacgccatcc agtgctgtgt
    14641 tcaaatcgct aataatctgg tcgtaaacca gatccttgga gtccctttta gggtaaagaa
    14701 gggggtaaac ctctgcaacg ttatcggctg ttatggtggt aaccacatca agcaccaggg
    14761 gaacatcgcc gtaaaggcga accaggtcga agtatgccca ggctctaatg aatgaagcct
    14821 cggctactat ctccttttta cgggcatcgg ttagtgcctg gtcagtaatt ttcggaacat
    14881 ttacgattac cgtattgcag tcggttacca ttttgtagag gtacttccag tcgcgctcca
    14941 cgttcttgtt ggttgcatcg atgctgtagt tatcgaactg tgaaaactcg gttccgctgg
    15001 caccggcata ggcatcgtca gactgcatat ctccgttgac aaagtaatcg agttgaaaca
    15061 gctcgcccct aaagttggag tagcaggtag ccagcagtgc ttctgcctgc tcggcagtgt
    15121 tgtattttag tgtatcattg ttgttatcaa cgcccagcgt gctgttggag tagtttgaaa
    15181 tggggtcctt gtcgagaaag tcctcacacg aaaccaccgt gaatgtcaaa agagcaacgc
    15241 cggcaagcag gtatccatta agttttttca tatctctcgg aatttaaaat tcgaacgaca
    15301 aaccaaaaat gtaagatctc gattgcggat aggtgccgta gtcaacaccc tgaaccacgc
    15361 tgctatttcc accgtagtta acctcgggat cgaatccgtt gtagcctgta atggtaaaca
    15421 ggttggtagc ggttgcatag agggtgaagt ttttaatgct cactctacta agccacttgg
    15481 aaggaagcga gtaggacagg gtaagagcct ttagcctgaa gtatgatcca tcctcaacaa
    15541 agcgggtaga gttgtgcaca ttgaaagtgc taccatcgct caccgctctg ggcatatcgg
    15601 taatttgacc cggtcgtttc cacctgcgca gcacctcggt tgtttgattt ttcacatcaa
    15661 acattccctc ggtgtcaatc cgcgaagcgt taaaaatatc attcccctga gtgccctgga
    15721 agaatagggt taagcctatt cccttgtagg ttaaggtttg atttaagccg tagatgaagt
    15781 caggttcagc tgagccaata accgtacgat cgtcagggtc aataacgccg ttgctattcc
    15841 gatcctcata aatcatatcg ccagtttcgg ggtcaactcc ttttgccaca tagccgtaga
    15901 atgttccaag agagtagcct ggactcattc gtatgacgta ctcgttgttg ctttcgagct
    15961 gggcaaagtt gtaaatctgc gataactgaa gctcggtaat ctcgttgcgg ttcatggacc
    16021 agaacaggtt tgtactccac ttcagttcat gcctgaagtc ctttgcatcg agcgaaagct
    16081 ccacacccca gtttttcatt tttccatcgt tgcgggtaat gtaatcaacc gcagcagaac
    16141 ccgatggaag cggaacatta accagtaacc cattggtctt cttccagtac acatcgccgg
    16201 tgaagctcaa atgcgagtct agcacggtaa ggtcaagccc cacgttggac tgataggtaa
    16261 cctcccattt gagtgaagga ttctccaggg tcatctggta aattccagga cctgaaagtg
    16321 ggttggttgg agactgtcgg gaaaaaccat aaagagcctg ccatgcgtag tcgcctattc
    16381 cctcttggtt tccattccga ccccatcccc cacgaatttt caaatcgtta ataaacagga
    16441 cattctttaa gaatggttcg gatgataacc tccatgcagc cgaaaacgag gggaagaaat
    16501 cccatcggtt atcagaggat aacttggaag aaccgtcata gcggaagtta gctgtaaaga
    16561 ggtatttact catgtagtta taggatacac gtccaaaaaa tgatgcaatt gcccattccg
    16621 aagcgcttga tgcggcatag gtaatctgat tagcagcatt aatggtagtt acagcagaat
    16681 ttttcggaaa gtctttagca gaaatgtacg attgatccca gcgtgaggat tgggctgact
    16741 gacctgcctg aacggagagg ttgtggttgc caaaggtctt ttcgtatgat agcacgttct
    16801 ctatcaacca aaccaaggag tttgacttat cggcctcacc aattccgttt tctgtgcgtc
    16861 cataaacagt tttgacaggg tcaatataga gatcgcgctg gtaaatatac ccatcaatcc
    16921 caaagtttga cttcaacttt aaatccttta agaaggcaat ttcagtattt gcgttggcca
    16981 gcacccgctc ggttttcgac tggttaatac gagaagcata agccaagggg ttttcccacg
    17041 aattctggaa tggatttacc tcgtactgtt cgggtttatt cggatcccag atatgaagga
    17101 agggtggagt gttaatggcg ctaaggatag tgcccgccct tccggagttc gcattatcgc
    17161 taacgtcgtt gtaccagtta tgaataaagc tgatattcgt tccaaccttt agccacttgg
    17221 ataagtcgct gttgatgttg gttcttatgg agtacttttt attttcagcg ggcttaacta
    17281 tgcctttttc atcggagaaa cctgccgaaa agtagtactt cattttctca gtaccgttgg
    17341 aaaccgaaac ctggtagttc tgaatttgtc cggtgctgaa ggtttcatta atccagttgg
    17401 tatagttagt ctgggaggca ggaacggttc ccggaccctt gatttcatcc atcagctccc
    17461 gatactgctc tgtgttcaga acatccagag aattagccag attagaaaag cccaaatagc
    17521 tgttaaccga aactgaagca actccggtct ttgcatgctt ggtggtaata attaccacac
    17581 catttgccgc acgtgcgcca taaattgcgg cggaagaagc atcctttaac acctgaaccg
    17641 tttcaacatc ctgcgggttc agcgcttgca tgcttgtagt aggaactcca tcaattacaa
    17701 acagggggtc gttaccagct aacaccgatg ttgcacctct aaccctaata attgcatccc
    17761 ctccgggttt acccgaaggt agcacaacct gaactcctgc ggcctttccc tgtattgcct
    17821 gtgccacgtt ggttaagggt cgtttgtcaa aatcatcagt cgaaaccacc gaaacagcgg
    17881 tggtaatgtc cttcttccgg ctggtgccat atcctataac caccacctcg gagatgtcca
    17941 agctttcgct ttcgagaact acatcaataa ttgaacgccc accaacaatc tcctcaacac
    18001 ttttcatgcc gataaaggag aattgcagaa cggcattttc aggagcacta atgttgtact
    18061 ttccgtccac atcggtaata gtccaatttg tggttccctt tacaacaata ttaacacctg
    18121 ccattggctc tccctccctg tcggtaacct ttccggaaac cgaaatggtt tgcgccattg
    18181 aagggatcca atacacgcat agtatgccca gaagaactat acgtgcacct tttctaaaaa
    18241 tctttaagaa atacttcata gctttagatt taaggtttga aacattagtt tactataaga
    18301 aatttggcgc ttagatttcc aaccgatatg agaaactccc ccggctcaac taacagctta
    18361 ccattgccat caacaaaagc cagctcgctg ctgctgagtt tgaaggttaa agttttagct
    18421 tctccggggg caagttcaat tttggcaaac ttcctaaggc gtttcacatc gggggtaatg
    18481 gtggctacta aatcggaggt gtaaagttgc accacctcct ttcctgttct attccccgtg
    18541 ttgcgaacat ttactgtaac ggttaaggtg tcattttgcc taaactcact tttactagtc
    18601 gccagctggc tatactcaaa ggtagtgtag ctaaggccga aaccgaatgg atactgaggg
    18661 ttatagtccg attcgtagct gtaggctcca atatttggct tttgctcctc agctggttta
    18721 tgatagtaaa caacagtaga gttagggtag ctggggtagg tgtagggtaa cctgccggaa
    18781 ggattaatgt cgccaaaaag aatgtcggcc agcgcattgg cgccatagct gcccgggagg
    18841 taagtttgaa ctaccgcagc catcaaaggc tcaatggggc taaagaccct tggtcgcccc
    18901 tcgtttaaaa caagtataac aggcttgccg gttttagcaa gttcctctgc caaattaagc
    18961 tgcttgcttc ccaggtataa gtcgttaagg tccccgggtt tctcagcata gctattttcg
    19021 ccaaggcaaa gaatgataac gtcggcctta cgagcggcag cagctgactc ttcgaactta
    19081 tcgtactgct cttcatagta tcgtccgtag ggcggatagc ttaccccggg gatatagctg
    19141 acctccgatt ggggtacctt acttttaatg gcctcaaaaa tggtattgaa cctgccggca
    19201 aattcctcaa ccttctcacc ctgccaggag tatgaccaac ctccatcaag ggttctcatg
    19261 gagtttgcgt tgggcccggc aacaagaatt ttatatcttt tatccttgct caagggcaga
    19321 atgccattgc tatttttgag cagggtaata gactcgccag ctgcctggta tgaaactttg
    19381 gcaaactcct cggagccagc cttagggtaa tcacccagca ctgtagttgg acgctcaaac
    19441 aaaccaagtt caaccttaag cttaagtatt cgcatcacgg catcgtcaat ccggctctcc
    19501 tctaccgagc cctccctcac cagctccgcc agcaggttac aaaaatcctt gtagcggtat
    19561 gggatcatcg ccatgtcaat accggcatta atagccaatc caatagcctc cttttcggtg
    19621 gcagcaaccc tatcccgccg gtaaaggttt tcaatgtcgt tccaatcggt aaccaccacc
    19681 ccgctaaaac ccaactcatc cttaagtaga tcggtaataa tgtaatggct ggcatgaaca
    19741 gaaatcccgt taattacccc ggagttaacc ataatcgttt taaccccggc atcaacagcg
    19801 gccttaaatg ggggcagatg gtattcccta agtgcgatgt ctggtagctg cgcaggggtc
    19861 ctgtccttac cggatgcagg caccgagtag cccaggtaat gcttggcaca ggcagcaact
    19921 tttagcggat gggaaacatc tacaccctga tatccatcta tcagcgcaac ccccatttta
    19981 ccgcacaggt atggatcctc tccaaatcct tcatactgcc tcggaaaacg aggatcagcc
    20041 cctagatcaa ggacagggga gaaaacccag gggattccgc ttgcacgcgt ctcgtaggct
    20101 gctatgcttc cgatttcccg aacgagtgaa tcgttccatg ttgccgccat gcctatttgt
    20161 tgcggaaaaa gggtagcctc tgccgtatag tttgcgccat gaactgcatc aatgccatac
    20221 agaattggta tagccaacct ggttttggtt acggccatat tctgaatggt actaataatt
    20281 ccgttccacg ttgtaaacga gcgtgccctg ttattgctgc tgttaagtat tgagccaaca
    20341 tggtagtcaa caatcgcctc ctgtagttca gccgtatcca gctcaaaagg ctcatagctg
    20401 ttaaacaggc tatccccctt cccaactaca tcaagtgtaa tctgagccat ctggcctacc
    20461 ttttcctcaa gggtcatcct gtccagcatg gccttagcca cctgctcagc atcgcttctt
    20521 ttttcaacat tgcaggagcc aaaaatcaat gccattattg taattcccaa tggccacaat
    20581 cttattctat acatattctt aggggtaggt acttttttgc tttataagtg tctttttgac
    20641 aatattaaaa tacaaatata agtaggtaat tattaaaatc aaataaaagt gtatttttaa
    20701 cacttatttt tcaaaaaaac aacttcggct atttttgaat taaattttga agataaaggg
    20761 gtggtgcttt tgtaattttt aaactaagtt tgcagccaaa tatacgttgg tatggatgat
    20821 gagttaagac taatacccaa cctttcgatt gattgtgtaa ttttcgggtg ggatgagggt
    20881 gtgctgaagg tgttacttcg aaaggagctg attgagtaca agaatattgt tcacatcgag
    20941 tggaaacttc ccggaaacca cgtggggatg aacgagcgga tagaagatac agccacgagg
    21001 gtactcaaag agcaagccgg tttagaaaac atctacatga agcagttcag ggtatttagc
    21061 gatttggacc gcttacgccg gcgtgagatg gactaccggt ggataaaaaa gaacgggctt
    21121 ggggaggata gggtgattac cgttgggttt tactcgctgg taaacctgtt aaactttgat
    21181 tcttctaaac ttctaaatgt tgccgaatgg cataatgtta acgaggtgga gagcttaatt
    21241 ttcgaccata aggagattct agacgaggcc ctaaaaaagc tcaaggagga tgtaaccatg
    21301 caccccatag tatttgagct gcttccgaag aaattcactc ttacccagct tcaacagatt
    21361 tatgaggtaa ttctgggtac gcgtttcgat aagcggaatt tccgccgcaa gatttctaac
    21421 ctcaagtacg tggtttcctg caacgagaaa cagtacggtg taggccataa acctccgtca
    21481 ctattcagat ttgacgcaaa aatttactcc aaaattcacc agaaaaatct cgacgctacc
    21541 gcttaacctc aactttaaag ctcatggtta ccgaaatcat cgggttgctg gccgcaatcc
    21601 tctctaccct aaaccagttt cctcaagcct acaaggtgat gaagacaagg gacacgcaaa
    21661 gtatctccct gtacatgtac tgcatagtgg aggtggcaat tgcgctttgg ctggtttacg
    21721 gaatactgct tagagatttt ccgcttatac tcgccaactc cctttccctg attcctatta
    21781 catacatttt tgtggtgaag ctcttccatg ttatttcagg aaaggagagc atctaagcaa
    21841 cttcctgtta atcattccat tttaaatgtg ttattatgac aattttcaag cattctcctt
    21901 attttttagg gctcaagcgg cagctaaaaa ttggcagtgg gggatgcaat tctgaacagc
    21961 agaccacatc caatctgctg cttcataacc tttgccggct caccccacac aagccaaaag
    22021 ggattttaac tacctgcata ttcctttcac attaaatcct tctcaactta tgttcaaagg
    22081 caagcggtga tatggtgtct tcattttggc taaaatcctc actcaaccct tccaacagca
    22141 gtaccattat ggtggttcct gaaaccgaaa tttcggcagt ggtggaacta tcatctaagg
    22201 atattttcaa aacgcctaat tcaattacct taaatggcta tgtatatgat atgcaaaaaa
    22261 atggctaagg aattagatct tcgccatttt taaatatcat ttgtatgcac taacatttat
    22321 agttaattac aggctcgcca atcggactaa ggcctcttta aattcatctc gcggtattgt
    22381 gatattcatc gccaaatacc tatgacactg cctatcatgg taaaatcctg aaagcggcag
    22441 taaaccaacg cctttctccg ctataaactt ttcaaccatt tcaacatcac ggatatcggc
    22501 gtagccttta taaccaagca ttaccatgta ccccgccgat tgctttacgg gaataagttt
    22561 actatccttg agtgtctcca gtaagagctt gtagttctca tccagaaaat cggctagcat
    22621 gtgcccttct atctcggcgc tcagaatatt gataaaatct tcggctcggt ttaagttggg
    22681 gttcgaggta atagccgaat ggatattttt ataggcaccc attaatggtt ctggtgcaat
    22741 acagtaagca atttcactat cgttaagtgc aacaggaata ttgagcgaac ctatacggta
    22801 agaaacctga caaagtcttg ggtaaaagtt aacgcttgtt ccctcagaac tgctaaagcc
    22861 cagttcagcc atagattcgt ttaggataag cttgattttg gtaccattta tcaacctttg
    22921 cagctcctca aaatcctcag ctgttagtgt ttgtccggtt aacagatgag gcgatgaaat
    22981 aaccattaac ttggttgtag cattaatggc tcgctgaacc tcgcaccaat caatgcggaa
    23041 atcgggggct cttaaaggta tgtaaacagg acgggcacca caaagctcta ctgctgtttt
    23101 taggtttgaa gtaaatggct caaacacaat cacgctatcg ccatccttta cggttgaagc
    23161 aattgctaaa aaaaccgcca ggttaacacc gctaacaaag gctatctcgg tttcagggtt
    23221 tatcgcccta aagtattttt ttagtatgta ctttgagagt ttctggtatg gattcatgct
    23281 ttcatcacga tccatatcaa gattgtaatc attaatatta ctatcaataa actcttgaaa
    23341 tcccttctcg ttgggtgtta aagaagtcat atcaatggcc ctgtactccc tgattagctt
    23401 ttgcaagctg tcgtcgctta aagctgttat ttcgcctacc ttctgggtca tagtgaatta
    23461 aaaacaatga ttgtagttta aaattacaaa tagtatttca attatcatat caattttgat
    23521 ggaatgcagg gtttatttga tgaatgcagc tattcgaata gctggattag aattggtagg
    23581 gttctgaaac gtattccaga accaaggtga aagcggtaaa atgaggcaat ggcttcaatg
    23641 agtatttgtc gctgattttg atttaaactc agcatttcag ggttgagcat gggtgtatca
    23701 atcaaattgc taagcagctt gctggtttgt ttctcaagca caaagccgtg agcgggttct
    23761 accccacaaa aaacaccgtt ttttatgtca aagtaaggct cttctggact ccatcggttt
    23821 aaggggtaaa accctaaata ccgcgaaagc tgcaccatga aaatcagatg aaagtttgct
    23881 acaccggtat tgaggttatc gagcatttga actgagctct caataaaggc aaagaggtgc
    23941 tggtttgttt cctgttctct taccgttctg taaattaact cgctgagaaa aatagtaatt
    24001 gtgagcttaa tgggatttag gtgaaggctg gtgaaagcgt atgtaggggt aacctctttt
    24061 accctgtaca tctcagcgtt agggcgatag tagtaaacca catcgagaat ggataggggc
    24121 tggaagtaaa cattcaaccc cgcctttttc cccttaggga aagcgttatt tactaacaga
    24181 gtaatacgcc catgctcacg tgcgtaacag ctaactatca ggctattctc cttaaatcta
    24241 acagtcctaa gtacaattac cctattgtgc tgtaacatgg gcttatttta caaaaagaag
    24301 tttagtaaca gcagcctgct cgccttttga gtcggagcaa aatattaggt aaacgcctgt
    24361 agcaaccctc tgcccgttcc tatttttccc atcccatgta gctaaacctc cgtttgagcg
    24421 agtctcataa acaatgtttc cagcaatatc ggttatttta accacgctat tgtccataag
    24481 cccggcaatg gtaataaccc cactatagtt agggcgaacc ggattgggaa aggcgtaaac
    24541 cttatcaaac gtattagctg gctcactggc attgctacgg taggcaatca agcccttatc
    24601 ggtggcaata aacacctcac cggtaaccgg atgcgcttta atgcttagta tcgtatttga
    24661 tggcaagggg ctattcttgg tgttaaaatg atgtatctgt tttgtcccat cggcagaaaa
    24721 aaggtaaacc cccgatttag ccgttccaaa ccatttacgg ttgcccccat caacatcaat
    24781 acacgtaacc tcctcggttt ccaagaggta aacagccaga ccttccacta catcgggtat
    24841 cttgattttt tggaaactgg tttcgccctg taatacccta tgcgggttgt aacttaccaa
    24901 aactccctgg ttggttccta accataaata accatccctg tcaaatgcaa gtgcagtaac
    24961 ctcgttggaa aataaattgc cattggcgtc gcgcggtcgg aatttaaggt agatatcgtc
    25021 acttgcggct tctacgtcgt taccggggct aaggacaaac aatccattat cgcgggctaa
    25081 agccaaccag attatccctg atggcgaaac ggtaagtgaa ataatccttg aggttccaat
    25141 tgctgagtaa tacggaaacg acttccatac cccatgtgcc gttagcaccg atatgggatt
    25201 tggaacctca gaatttgcta cccaaaggtt tcccttgggg tcaatatcca taccggctat
    25261 tctacagtat ggttgtcctg gaaatacact ttgcagggtg ctattatcgg gattataatg
    25321 ctccgccagg gttccactat taagtttata cagacctcca ccccagcttg caacaaaaaa
    25381 aacattcggg ttacggggat cagccttcac cctaaccgca tcgtgattaa accaatcagc
    25441 atagtacgac catcggccat cggaaaaagt atgtgcaata aacgggttcc aaaaattccc
    25501 gaaagcggca tcgtatgcac ccgatgccgc cacaaccatg tttgcacttg catcaatgtg
    25561 aaaaaatttg ttgctaaacg ggcccgttgg agtatgaaca ctatgatctg taggggatat
    25621 aatcactaaa ccattgaatt catcggcaac agccaaagca ttgttttcaa gcggtataca
    25681 atcggcaact tttggagtgc caccaaaaag gtaactgctt acaagttcta acttctcgcc
    25741 ctgcaagcta tatttctgaa tagcattact gctaaaggca gcaattttct gaaagcctat
    25801 ggatacacca agggtttcgt caaaaggcct atcaacctca acccatccag aaccagaatt
    25861 catccataga atatcgttac caccaacttt ttccaaagcc aacaagtggg tttcatcagc
    25921 tgtaacccct ctgcactctg ctgcagggtc agtaaagaat tgctccctgc tccatcgttc
    25981 ataacttatt agcaaagggt catttgtact tgcgctgtaa acaccctggc ttgtagctgc
    26041 ccaaaacttt ccattccagg caaccaacct gttgaccttt aaggtaccag cgttttcaac
    26101 aataaaatag gtatccttta cctcaagccg atcggggtca attaccacta aaccaaaatc
    26161 ggttgaaacg taaaccaatg agctgaagta gtaaaaatga tttatgcact tgctgcccaa
    26221 catgggctta tccttaatga atggcaagct gacgacccgc ttggtgtttt catacactaa
    26281 atcaatgttg ccagatttat acccaactaa cgttatctgc ttttgtggta ttgctgataa
    26341 tgcagtaata tcaacctcgc ttaatccgga aacagttgtg tacttgttga gctcgccgtt
    26401 ttcggtatcg taggtaaagt agccatttgc cactgcacag taaagctttg aaccgctaat
    26461 gctaagctca cgggcatcgt taaaggcata gtaatcgcgc cactgcccta tggcaggttg
    26521 ggtgtgaacc ttgcaaattc ctacaaatat gaaggataaa aggatatata tcctctttac
    26581 catagctaaa gatttatgtt acccggtgta actaaagaat aaacgattaa tgattgatta
    26641 atattgctaa aacctgcttt ttaggaattt gcataactcc tcaacggctt ttcccctatg
    26701 gcttatttga ttttttagcg ataaaggcat ctcggcaaac gtttcggtaa agccatcggg
    26761 tacaaaaatt gggtcgtagc caaatccatc ggctccccgt tcctggtcaa tgattttccc
    26821 ccaaacaact ccctcaaaaa gatactcttc gccgttaagt attaatgcga taacagttct
    26881 aaatcgagcc ttacggtttt caactccttt aagattacta agcaatttac gtatgttatc
    26941 cttcggtttt tttgcatcgc cagcatagcg tgcactgtac acacctggct cccagtttaa
    27001 cgcctcaacc tcaagaccgg tatcatcggc aaaacagttt tgaccggttt tgttgtaaac
    27061 atagcgtgcc ttttgcagtg cgttatcctg aagggtacca tattcctcgg gtatttcgtc
    27121 ggtaatacca acagcgttta acgtaaccag ggtaaatcta tccccaaggg catgctgtac
    27181 ctctttaagc ttgtgtggat tgttggtggc aaacacaagc gaaaaactgc taccggccat
    27241 aagtgaatta tcggggatta atactcatta cagggatatg ccttgcattg gccagaatat
    27301 attgctcctc gggttcaaca atatatttac catccttact ggttggagtg gtcataataa
    27361 ttattaggtc agctctaata ttaacggcca tgtccagcac ttcctcctca tacttcttaa
    27421 tgccgggaac ggtatactcc acgtacttgg cgcccctctc atctaaaaat ttcttggccg
    27481 aagccaggtt tatatcaatc tgtgccatca acttgggatc cgatgtattg ggcttaatga
    27541 tatggaaacg ggataggtag taatcacaga agtacgatat ccactcatgt ttctctctgt
    27601 tttcaatggt aaagtccaca gggaatacaa tatcatcgta acgcttgtta accggtggtt
    27661 cctgtatggt tataaaggga accttgcagc tggtaattaa ctttagcacc cacgaaacct
    27721 tatgctcatc catctccttt acggttgatg agcccataat taccatactt gcatttattt
    27781 catcggctgt tgaagctatg tcggtcatgt agttacctac tttgactatc gattttggag
    27841 taacagcgta cttgctcttt accgattcga taagtgaagc cattttactt tcggcttcag
    27901 caagtttttc tggctccgaa acacagtgta agatgtgaat tggctcctta acaacttcag
    27961 aaacccttat ggcatgctgc agggcaaacc atgcaacttc cgtaaaatca gttggaacca
    28021 aaattgattt tttagcagtc ataattgtcg ttatttggct tttcccctaa aacattacgt
    28081 tcataaatca ccttcaaatt taatcaaatt attattttta ttgagttcaa aagaaatttg
    28141 tttaaacaaa ttgttaaaat atttgataaa gattcgcaat cgctctatct tagtatgtga
    28201 aattttttgc gttttaccaa atggctttcc tttcaaccct aatagcgttt tctttattgg
    28261 cagtagcaac tcttgctgct atgatgctgt atgtacgtta ctactacacc aaaaagagtt
    28321 ttgcccttca gcagaagctc tttgttagaa ctgaggaatt actcagggaa agacagaaaa
    28381 ccgaaaactt gctctcacgg gttttgcccc gcgaaaaggc taaagaacta attgaccagg
    28441 gaaaggttga tgcacagaaa tttcagatgg ttacagttct ctttgccgat attcagggct
    28501 ttaccaaaat aaccgatgaa attaatgccg attcactcat tgaccagctc gacaggcttt
    28561 tctttgaatt cgatagcata gttgaaaagt atggtattga aaaaattaag accataggcg
    28621 atgcctatat gtgtgcaggt ggcattcccc atccaaacag aaccaatcct tttgaggtgg
    28681 ttgctgcagc agtggagatg atgggttgca tgcaaaagtt aaaccaacag ctcaataacg
    28741 gcaaagaaat ctgggagcta agaattggta tcgataccgg gcctgttatt gccgggattg
    28801 ttggtcgtaa taagttgacc tatgacatct ggggaaccac agtaaatatt gctagccgaa
    28861 tggaatcggc aagtgagccc ggaaaaatca atatttcgga aaacacctat ctggttgttc
    28921 gcgatctatt cgaatgcaag ttccggggaa aaattccaat caagaacaag ggcgatgtta
    28981 acatgtactt tgttgaagga ttcctaccta aattttccga gtccagcagg tatatcccaa
    29041 acaatgattt tagggttgag cttcagcttg taagattagc cgatctggaa gaatttgttc
    29101 ttgagaagct ggaaaaaggc cttccaaaaa atctttacta ccacaattta aaacacactg
    29161 tcgacgttta tacacaagtt gaacttattg gtcgagccga aaatatacct aatgaagatt
    29221 tgctcttagt tagaacggct gccttgtttc acgatatggg ccacttaatt gactatgcaa
    29281 cgcacgagga aatgagcgtg aaaatggcgc atgaaatatt gccccgctat ggctatactc
    29341 ccgaacaaat tgctcgggtt gctgaaatca ttatnnnnnn nnnnnnnnnn nnnnnnnnnn
    29401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29461 nnnnnnnnnn nnnntgcccc gctatggcta tactcccgaa caaattgctc gggttgctga
    29521 aatcattata gctaccaagt tacctccaaa acccaaaaac atcctggagg aaataatttg
    29581 cgatgccgat ctggattacc ttggccgaac cgattttata cccgtatcga atgctcttta
    29641 caaggaactc catgagcatg gaatggtggg ttccttacac gattggaatt tgattcagat
    29701 tgatttcatc cagaaacacc agtattttac tcaaacagcc cgtagattaa gaaatgtaaa
    29761 caaggatgtt cagctcaaaa atctaaagcg atggatgaac gaattagaaa atgatacatc
    29821 tttgtagtga tttttacatt tttttgtatt acttttacta ttttgttaaa tatttgctat
    29881 tttgcatagt taaagctgaa atactgacca aaagttccgg ttatggataa tattaaaaaa
    29941 attattttgg ttccctggga ttttaccgaa ggttccgaaa ctgcctttca acatgccctt
    30001 cagctcgcaa aagccggagg taatgagatc atgttgctac acatcatccc ccgtgcctgg
    30061 attattgact ccatataccg taaggtaaat ttcaagaaga tagaaaccta taatgccaaa
    30121 attgaaaagg ctgcttcgga gcttgaacat aagtatggtt ttaaaccaca aacccttgtc
    30181 ctggaaggat accccaaacg tattatcaaa gagttaattc ttacagccca tgttaacctt
    30241 atagtagcct actatgccta taaatttggt agtaaagagc accgcatatc acgatacctt
    30301 aaaaatctat ttttcaagga tacaaccctt cccttcattg tagtcagcaa agccccaatc
    30361 cactctcact atattgaaat tgtaatcccc gtagattacg accggaagtt caaggaagca
    30421 gttcactgga ttatttacct tgccaagtac tacaagtgta acgttaacct cattaagcct
    30481 ttcctaaccg atgatggcaa aaagaaacag ctggccaaca atatctactt caccaaaaaa
    30541 atgctcgatg gggttaatat tgtttacggt attaaaaccg ccaagaaaaa gggaactttt
    30601 gaagaggaag ttttccgctt tgcccgcaca attgaagccg acctggttct tatcatgtcc
    30661 gacaagtatg acgatttcct cgatagcaag caggaagcta aacatgatat ccctgtaatg
    30721 tgtgttaatc ctcgtattag aaagtatcaa agtttttatt aataggtttt tatgaagcta
    30781 ccaagaacga tgcttttaac ttcaatcatg aagttgtcgt taaaagaaaa aattattttt
    30841 ctattcaccg gaacatcgtt tcttattctt tttttccttc ttttcctaaa tagcgctagg
    30901 tttaaccgcc tatccattga aacagccaaa tcgcgcattt cccttttagc cgataaggag
    30961 gtaaaaaaat ttgaggcaac actggaaagg gatatgtctg ttgcacgcac cattgcctcg
    31021 tcaatggcag taaccataaa agaggggaag acatcaaata tacaggaata caacaatctc
    31081 tacaccaaca tacttgagca ataccccgat gtactttccg tatgggatag ctgggaactt
    31141 aaatttgttg aacccggata tacctacgaa cacggccgca ttaaaaatta cgcgtggcgc
    31201 gaaggcggta agatactggc aaatactata agaactgaaa aaactgaatc gtcagcggag
    31261 tatatcactc taaaaacaaa caaatcggaa agtttggagg aaccctactt tgacaactac
    31321 gagggacaaa ccgaaaatat tctgatgacc agcgttattg ctccctgcat tatcgatggt
    31381 aattttgccg gggtggccgg gctcgacatt cccctttcaa actaccatca ggttgttaag
    31441 agcattgtac ccttcaagga tagctacgct tttatcattt ctcctcagct caagtttatt
    31501 gcccatcccg ataaggattt gattggtgac gatgccctgt tttcctacga gtccatattt
    31561 acttcacatg gcgttcagga caaggttata aatggtgaac ccattgaatt tgaagcaaca
    31621 gatatcaacg gaaagttgtc attctttact ttccgcccca tttcagttgg taagtgccca
    31681 caacactggt cttttgtact gattacaccg cgcgaaacca ttttagcaga ggctcgccat
    31741 gttttttgga caacccttac tattggttta ataggcattt tgttgctttc tggctttatt
    31801 taccttatga cccttaaatt ccttatcaat ccaataaata aagttaccca aaatttagag
    31861 cgcctctcta aaggtcatgt tgacaacagc atgattgttg atgttgacaa caacgacgaa
    31921 ataggcacca tgattaccca cctgaacaaa accgttgagg gcctttacaa aaaaaccgag
    31981 tttgctaaac aggttggcat gggtaattac gacacctcac ttgaactcct cagcgatgag
    32041 gatgttttgg gtaagtcgct gattgaaatg aatgagaatc tccgcaaggc aagggaagag
    32101 gcgctaatca ggcagcgtga cgaggaaaaa cgtcgttggg ttaatgaagg tttagccagg
    32161 tttggcgata ttctccgaca aaataataat gacattgagg ccttaagcgc tagcattgta
    32221 aaaaatttgg ttggcattct taacgccaat cagggcggac ttttcattta taacgatgag
    32281 aacaccgatg agccctactt tgagctaact gccgcttatg cctataaccg tcaaaaattt
    32341 attaaaaaac acatcctact tggggagggg ctagttggca cctgtgctct tgagaaacaa
    32401 accatctact taaaggaaat ccccgagaac tacatagcaa tatcctctgg gcttggtgag
    32461 gcaaaaccac gcaacctgct tattgtaccc ctaaaggttg aggataaaat atttggcgtt
    32521 cttgagctgg catcgttcaa tgagtttgag gactaccaga ttgagtttgt tgaaaaggtt
    32581 gcccagagta ttgggcaaac acttcagtcc gtacgaacta acattcgtac agcggaactc
    32641 ctggctaaaa cacagcaaca ggccgaggaa atgaaggctc aggaggaaga ggttaggcaa
    32701 aatcttgaag agctggagac cattaaggag gagctagaga agcgtactgc cgaaatggag
    32761 gaaaaccaaa aacagctgga atgggaaaaa tcgctgttgg actccctgct taactacctt
    32821 cccgataaaa tctactttaa ggatctaaaa agtcgattca ttaaggtaag caaatcaacc
    32881 ctcgaattct ttggaatgtc aaagcaagaa gagcttatcg gtaaatccga cttcgacttc
    32941 tttactgagg aacacgcccg ccctgcttat gaagatgagc aacgaattat tagaaccgat
    33001 acgcccatta tcggtattgt tgaaaaggag gtaatgaacg acggtagggt aacctgggct
    33061 gagacctcaa agctccctct aaaaaattcc catggcgaaa ccattgggac ttttggcata
    33121 acccgcgaca ttaccgcttc caaaatgatg gaggaggaaa tttcaaagaa atccgaaaag
    33181 gaagaagcct ttcagcgcga ccttgagaaa aaggtgattg aacttgaaaa cctctataaa
    33241 gccataaacg attccgctta cgttattgag tatagccctg aagggaacgt taccttcatt
    33301 aacgaggctt accttcagct gcttaacctt aacgccgatg atgttattgg gaagcaccac
    33361 tcctaccaaa tggaatttac tgatgaacag cgtaaaaact acaaacagtt ctgggacgat
    33421 cttaataatg gcattgttag aaaggaaacc tcaaagttca gcattaacga taaaacctac
    33481 ctgttctacg aaacctacac cccaataagg gatgctgaag ggaatgtggt caagatcatg
    33541 aaaattgctt tcaacgttag ccatttgctt tccgaaaatc aaatataaag ccgggtttac
    33601 ccggtttttt tatcctgtaa tctatgttaa gctggaaaaa acttttaagc ccaataagaa
    33661 cgggcgaatc gccatcaaaa tcgactattg actatacccg aaccaacttt cagcgcgact
    33721 atgataggct gatcttttcc tccccgttcc ggcgtttgca aaataaaacc caggtttttc
    33781 cactcccggg tagcgttttt gtgcataaca ggctaaccca tagccttgag gtctccagtg
    33841 ttggccgatc gctagggcgg agcatactaa accttttatc acccgaaagg tttgagtgcc
    33901 cagcacagct aattaccgat attcctacta ttgtttcggc ggcttgcctt gcccacgatt
    33961 tgggaaatcc tccctttggc catagcggcg aggatgcctt taggcatttt ttctccaacc
    34021 atcacattcc tgaacttgat agctgggaag caagcgacct gctcaggttc gagggaaacg
    34081 caaatgccct ccgcctgctt acccaccagt ttaggggccg acgaaccggc ggtttccgtc
    34141 ttagctatgc aacccttgcc tcaattgtta agtatcctta cccttcaaca ttgaaagaaa
    34201 aaaagtttgg ttattttaaa tctgaagccg atgtttttaa cgagatagcc agtcaaacgg
    34261 gtcttattca gattagcaat ggaagatatt gccgtcatcc tctggtttac ctggttgaag
    34321 ccgctgacga tatttgttac caggtgatgg atattgagga tgcgcacaag ctaggaattc
    34381 tttcaaccag tactgtaacc aacctttacc tctccttttt tgacgatgaa accacaagta
    34441 agaagaaaga tattcatgaa accctttcct cggttaacga tataaacgaa cgcattgctt
    34501 acctaagagc tattaccatc agtaaactta ttgagcaatg ttcttccaac ttttcaaaaa
    34561 gctatgacca aattatgtcc ggccagcccg taaagccgct tattgaccag atggaaggag
    34621 catctgcaaa ggcaatggac gaaattcgga aggtttctgt taaccaggtt tacaaccatt
    34681 ccaaggtggt tgaaattgaa attgctggct ttaccattat tggggaacta ctcgatattt
    34741 tcacaagtgc agttcttgag cctaccaagc cgatttcaaa aaaagccctc aggctgatac
    34801 ccgaacagta ccttgctgaa agctccgata attactccaa ggtgatggct gttgttgatt
    34861 atatttcagg catgaccgat atttatgccc ttgaaaccta tcagcttttt aaagggatta
    34921 aactaccctc gctttaatct tacttctcgc ggcttaccat ataatcagca aggagcaata
    34981 gtgatttctt atactccgaa tcgggaaata tttcaagtgt ttgtttagca ttatccctaa
    35041 actcaaacat ttttttggag gcataatcaa ttccgccctt gtccttagca aaatcaacta
    35101 tctcagcaat gtttgctttc ttcccactat taattagtct gataattcgt ctcctttcat
    35161 gggtatcgga ttgggaaagt gcataaatta aaggaagtgt aagcttgtgt tccttaatat
    35221 cgttaccggt aggcttgcct attaaccctg atggctggta gtcaaaaaga tcgtcgcgga
    35281 tttggaaagc catgcccaga tatttcccaa actggtacat tctatcaacc atatctttgg
    35341 tcgcattggc tgatattgcg ccattggcgc agcacgaggc tattagcgat gccgttttct
    35401 ttcctatgat atcgtagtag gtttcctcat caatattcat ctttctggat cgttcaatct
    35461 gcaaaagttc gccctcgctc atttccttta cagcactact aatcaccctg agcatctcaa
    35521 cctgaccatt ttcaatagca accagcaaac cccgcgacag aataaaatcc cccacaagta
    35581 ctgctacctt tgaacgccat agggcattta ttgaaaaaaa tccccgccgt tcataagctt
    35641 catctaccac atcatcgtga accagtgttg cagtgtgaag caattcaatc atccctgcag
    35701 ccacgtaggt tgactggttt atttggccac aagccccagc gctcagaaat acaagcattg
    35761 gccgtaactg cttgcctttc cgcctcacaa tatagttagt tatcagatcg agcaatttta
    35821 tatcggagcc catgtttttt ctgaaaaatg gctcaaaatc attcaaatga ttacttattg
    35881 gctcctttat ctggtttagc ataaatcttt atctattaaa gggcaaatta accaaatttc
    35941 gcccatacta aacactgttt acccaaacaa tgtttaaaaa attgataaat accaattggt
    36001 ggtaaaacaa aatattaata tatttgcatg tgtttaatat gattgaaatt cagaacaata
    36061 atggtacagg ttgaagaatt gaagattgag cagcttgagc aggctgcaag catgttgaaa
    36121 gctattgccc atccactccg aattggaatt attagcttgt tggaaaaatc ggggcagttg
    36181 accgttaccc aaatatatga aactcttggt atagagcaat ctactgcatc gcaccatttg
    36241 ggtattttaa aggatagggg tgtactttca tgcaagcgtg agggcaaaaa cactgtatac
    36301 ttccttcgga atgagaatct gggtaagata cttgactgtg taggaaggtg tactgtttaa
    36361 taattaaatt atgaccactt ttgatatcat aattgtaata ctactattgg ttggtgcagt
    36421 taggggtttt attagtggcc taattatgca actcacatcg ctggcagcgc ttttacttgg
    36481 catttgggta gcaatccggt tctccgattt tactgctatg cttttaatgg aaaaggctgg
    36541 tttaacaggt cagtacattc ctttagtctc atttgccatt acctttgtgg ttgttgttat
    36601 tgctgttcac tttaccggaa aactaatcga taaatttttt aacctaaccc cactgggtat
    36661 agttaataag attcttggct tggttttcgg tgtactgaaa tatgcattaa taataagcgt
    36721 aattattgtt tttgttgaaa aagccaatca gcggttcaac ttttactctg aacaaaccaa
    36781 aaataaatca atgctattta agcccctttc aaagcttgcc cctagcattt tcccttacct
    36841 tcactttgaa tcgatttctg agcgcttaaa agcgaaataa tgcaccattt agtcagattc
    36901 atttcggcta tttactttaa atgaactata tttgaaggcc aaatcatttg gccttttttg
    36961 tttaatcact taaaatggtt tgccgtgaat tttgtagagg agttaaaatg gcggggcatg
    37021 gtacacgaca tgatgcccgg aaccgaagag cagcttacta aggaaatgac tgccggatat
    37081 attggctttg accccacagc ggagtcgctc catgttggta gctttgcaca aatattgctt
    37141 ctaaagcggt ttcagctagc agggcacaaa ccaattgcac tggttggtgg tgcaaccggt
    37201 atgattggcg accctagtgg aaaatcgcag gaacgtaacc tgctcgatga ggaaacccta
    37261 cagcgcaacc tggagggtat taaacgtcaa ctttcaaagt tcttggattt tgagagcggt
    37321 aagcccaact cggctgtcct ggttaacaac tatgactgga tgaagggtta cgactttctt
    37381 agctttatcc gcgatgtggg gaagcacatc actgtaaatt acatgatgtc caaggactct
    37441 gttaaaaaac gacttggcga ggagtccaag gaaggtatgt cgtttactga gttctcatac
    37501 cagcttgttc aggggtacga tttcctttgg ctataccaaa acatgaactg taagctgcaa
    37561 atgggtggtt cggatcaatg ggggaatatc actaccggaa cagaactcat tcgccgtaag
    37621 cttagcggcg aagcctttgc tttaaccatt ccgctcatca ccaaagccga tggcactaaa
    37681 tttgggaaaa ccgagagtgg taatgtgtgg ctcgatgcac gcctcacttc accatacaag
    37741 ttctaccagt tttggcttaa cacctccgat gaggatgccg aaaggtatat caagattttt
    37801 accctgctcg acaaaaccac catcgatagc cttattgctg agcataaaaa ggagccacac
    37861 ctacgtaagt tgcaaaaaac acttgcccgc gaggtaactg ttatggtaca ttccgaggac
    37921 gactacaaca aagcagtgga agcatcggaa atgctatttg gcaatgccac caccgatatg
    37981 cttaaccgga tggatgagga tactttcctt tccgtttttg agggtgttcc cacctttgag
    38041 gttaaaaaat cggagcttgc caagggtatt cccttttcag ttcttgctgc cgaaatcaca
    38101 aaagtattcc catcaaaagg ggaacttcgt cgcctggttc agggtggagg gttaagtatc
    38161 aacaagcaaa aggttgacaa tcccgacatc acaattggta ccgaaacatt actcaacggc
    38221 aaatacatac ttgttcaaaa aggaaaaaag aattacagca ttatactagc tgttgattaa
    38281 ttccgttata tatttcggaa aatctttgaa ggcttgcagc attgttttaa ggcaagcctt
    38341 tggttttctt taggcaaatt atgaaatttg gatatcaagc attctaattc atcagctatg
    38401 gacaataacg atattagcat ggacaaatca atgctttcca ttgctcaaac catatggaat
    38461 ggccgaaaaa cgataattac tgtaggaatt attgcgataa taatttccac ttttgcatcg
    38521 cttttagtaa aaaatcaata taaagccact gcaaccatat tccctccggt ggcaaatcag
    38581 gtgtccaagg aactgattac cgaaaataaa caggaaggtt taacaacctt cggcgaaacc
    38641 gaggagatgg aacagttcct tcaggtttta agctcacgta ccttaaagga taaggtaatt
    38701 tcaaggttaa acctccagca acactgggga atcgactcca catcaaagag ctataggttt
    38761 aggagttacc aaaaatttga tgaacaagtc aaaatgaaac ctactcgcta tcaaagtatc
    38821 aatattgagg ttatggatcg tgaccctgaa tgggcagcca aaattgcaaa tactgtggtt
    38881 gagtattccg atagcctaat gcgtgccatt aaagcacaag tggctgttaa tgccttaatg
    38941 gcattggaaa agcaatttgc tttagttgag catgatttaa ataacttaca ggattcgcta
    39001 gcacttgcca tatctaatgg ggttgttgac cccaaacgac aggcaaccat attctacaag
    39061 gaatacataa atgcattact aaaagctaat aatgctcacc tcataaaatt gaaaagtgag
    39121 atgtatcgat ttagtgatca cggtgcaaag agtatacgtt acgctgcaga aattaaagat
    39181 ttggcagaac gtttaaacga gttacgaaaa agcatagttg ttgctagaat tgaggcaaat
    39241 caggagatac ccacccagtt cattatcgac aaagccgata tacccgacaa gaaagcctat
    39301 ccaaagcgca gtataattgt tgtaacctca accttagctg caataatttt tgcaattttc
    39361 ttgcttttcc ttcaggagta tcttaagcgc ttaaaaatat aattgttgat accttttgca
    39421 ggatcagttt accccctaat ggatatcatt aaatcgatat taagccatat taaaaataag
    39481 tggataattg tttccagcat aatcttcatt ttaataaatg ctattctgat aggaactgag
    39541 cgcgattttt ttgtaattct acccatcata tttatttttt tactggctta cctatctttc
    39601 cccttaaaat tctacttggc ttacttattt ataagcccgc tctccattcc tctatcggaa
    39661 tttttccagg aaatatccat tgatttatgg ttaccttcgg agttaatgct tgtggcatta
    39721 gctcctattg ccttaattat gcttttatca aataatcatc aaaaccgttg gtttacttgg
    39781 catcctgtac tgctaactat ttactttttt ttaggatgga ttgctttaac ttccattacc
    39841 agttcaatgc ccttggtttc cctaaagtat ttaattctca aaacacttta cttcatcgtt
    39901 cttctttatt tacccttcat actacttgtt aaggggaata ttacattcaa acgatttatt
    39961 gctttctact ccataggttt gtttgtagta attctgttaa ctctatcaaa gcaatgggaa
    40021 tatggtttgt tcaataagtt tgtagcccac ggttcatgta atccattctt cactgaccat
    40081 acatcatatg cagcagtatt agctttgcta attccagtta gcattgctct actcatatca
    40141 tccaacacgg caacgcggag aatacttttc cttgcattaa caattgttct tattgtagcc
    40201 tttgtgctat cgtacacaag agcggcctgg cttagcttaa tggtgacagg tgtagtgtgg
    40261 gttgcatggt tgcttaaaat tcgattccga tatttagctc tgctgacaat aatttttttt
    40321 gggtcgatat tctatttcca ggacaacatc ctaatttggc taaaccagca caaaaccgca
    40381 tcgagcggtg atattagaaa gcatattagc tctatcgcca atattagaac cgatgaatcg
    40441 aatgttgaac gccttaaccg ttggacttcg gcaattagaa tgtttgagga aaaaccgctg
    40501 cttggttggg gccccggtac atacatgttt cagtatgccc cctaccaggc ttcgtatctt
    40561 aaaacccggg aaagctcaaa ccttgggcta aaagggaatg ctcatagcga atacctagga
    40621 ctgttatcgg aggcgggtct tccagcagct ttggcattta tcctcattat tggcatactg
    40681 ttttacaaag gttttaacct agccctaaag ttaaaaccca aaagcgctga cagggtaatc
    40741 ctaataggcg tattacatgg gttaataacc tatgatgtgc atggttttat gaataatttt
    40801 ctggatatgg acaaggttgc agttcttttc tggggttatg ctgctttaat cctggccatg
    40861 gagcaccttt actcaaaatc gaatcctaga atcgaaatat catcaaaaac atccgcatcc
    40921 gattccctct cctgagcaat ggtgtttatt atttgctgca cactatggtt tcgcgaaaag
    40981 ttcttttcta aaatccttgt gttgtaaacc accttatcgc catcaatggt ttcaactagt
    41041 ccatcggtgt aacagagcaa agtgcttggc tcatctatat ttagaacgcc ctgattgatt
    41101 attggtatat catcaatcat tcccaaccca acacatcccg atgaaagctg aattatttta
    41161 ctggaatttc ggacaaacat gaagggtgga ttgtgaccag cattaacata ttccagcttt
    41221 cgggtttggt tgttatagcg gccaataaac atggtaataa acttttcgcc tttagccgta
    41281 gccattactc tctcattgag tttttcaacc aacgctgaaa ggctaatatc cttgctgaat
    41341 aacgccctga ggtttgcctg gaagtttgac attagcaatg cagccgaaat acctttaccc
    41401 gaaacatcag ctatacagaa acccagatcg ttagggctaa gggctataac atcatagtaa
    41461 tcgcctccta cctcaaagtg tgggttatag taagatccaa tgctaacacc attgaatttg
    41521 ggcatatcgg caggcgaagg aataagcatc gactgcatac gcgatgcaag ttccagctcc
    41581 ttacgcattg cctcctgctc caagctttta tggaataaac gcaggttttc aatagcaaca
    41641 aaaatgatga ttgatagggt ttgaacaaag ttcaaatggc ggatggttgc gcttacccct
    41701 ttctcgtatt cattggtatc gcctatcagc acatacccca ccggctgatt attctgaatt
    41761 accggtatta ttacatcaag ctcgagtagt atgggatttt ccgatactcc tacaaaggtg
    41821 atctcgttaa agtgaagtaa atcgcgctct acatttatat tctcaacaac cctttcgggg
    41881 caattgctat taagtagcag ttgccacttg ccctcctcta tcttgtaaag caatatcctg
    41941 tcgattccta aatcatcgtt gaggatacgc ttaaacctgt cgataagctc ctcggttttc
    42001 aactcagcgc ttatggcctg agccatacct aggagcgcgt ttagcttaaa cgtacttagt
    42061 tttaacctat tttgcgaggc ggttatcgac atacaatagt tttaacctaa aaaaataact
    42121 tattccaaaa gaggaataag ttactacaaa attactaata aaacaatatc cgcctaaatt
    42181 attttgaaat tactttaccc tttccgagta ttcccttgta cgggtatcaa ttttaatttt
    42241 atcgccaatg tttataaaaa ggggaacatt aattacggca ccagtttcaa cagtagcagg
    42301 tttaagtgct gttgacgagg cggtatcgcc ttttagtcca ggctcagtgt aggttacctc
    42361 catttctaca aatggtggca gttcgcaggt aagaatattc tcattatcgg cctcaaccat
    42421 catttcaaca tactgtccct ctttgagtaa atcagggttc tcaatcttgt actcatcaag
    42481 tgatatttgc tcaaaggttt cctggtgcat gaaatgataa cccaactcat ccttatatag
    42541 gaattgataa ggcctgcgct caacccttac aacatcaatt tttacccctg cattaaaggt
    42601 gttctcaatt acttttccgt tctcaagact cttaagcttt gtgcgaacaa atgctccccc
    42661 tttgccgggt tttacatgtt gaaattgaac gatggtgtaa atttttccgt tgaataaaat
    42721 gaacagtccg ttcttaaaat ccgctgtagt tgccatataa ttctatttta caatgggttg
    42781 caaatatagt aaaattgaaa taataaaaaa attaataact acctgttaac ataaacttta
    42841 ggtagatagt tgatgtgtaa aattgatgaa attgggggtt acttcttctc tagcgcagta
    42901 aattcatttg ccaggttctt gtattctatt aaatcgcagt aaactgaacc aacaaaaata
    42961 tcaaacttta ttctgattgg gataaagttt gagtcgttgg aaaaccatat ggtcatatcg
    43021 tccttggttt taaaaaccct accggttata acaacaggca tgaacttcat gcactttacc
    43081 aggccaaatt tggtttttat ggtctcatat cctttaaaac ggacggtaag agggtatttc
    43141 tcatcggtga aataggtatc aaccgtaatg gtttgatctt gctttagggg tggaatggtc
    43201 agcatcttgt ttctaaggta gtataccgat gaaagaatat cctggcaccc cttaactaca
    43261 accaccttgc cggtctttga acttataaca atagacgaat cggggcgact ccagtgatcc
    43321 caggtttggg tgctgtaatg cctgtaacga ccctcatgaa tgtttcggat ggaaaagtat
    43381 ggccaatgtg tggctgggtc ggtgtagctt tcgtaggtat cgtttacgtt gtaaagcgtg
    43441 tttgctattc caactgtccg cccgtttaaa tatagatggt aggctggctt accattgaat
    43501 acttgctcat ttacctgtaa ggttgcctcg cctccattta taaatccata gtaaatctga
    43561 tactttaact tttctccacc tataaatgca ctctttccat tctgggcgtg agcattcata
    43621 ttcaaaatgg aaatgaacgc taacacaaaa ccattaagaa ttgggaataa tcttattctt
    43681 aggggttgtt gccacaaaat ccttaaggta gaagggttca aaatatgctg tattctcaaa
    43741 ttcttcattt ttgtatgcat ttaatgctat gggcagcata aagcgtgccg aaggctcaaa
    43801 accatcgata aaaatagcat ttttactttt aattacatcc ttgcacttgg ctgagccatt
    43861 cccaaagaaa ataatttttt ggttttgtaa gaattctgca aaagagtttt catcaacaac
    43921 tactgccctt acatcggaaa caggctcacc ttgaggatta tagagcgcgc tgtaaacttc
    43981 catgcgtcga gcatcaatca ttgggcaaag tattgcatca gcaggtatat tgggatggga
    44041 tagagataaa cctaccgcca ttgcctgtaa gctacaaacc ccaataagcg ggattcccga
    44101 tgtatagcaa attccctttg ccattgaaac accaatcctc aaaccggtat acgatccggg
    44161 gcctttgctt acagcaaccg cactaatttg gcttggcgtt aagctagcat cggttaacac
    44221 atcgttgata aggcgtggca acatggttga atgagccttg gggtcagaaa gctctctgac
    44281 cccaagtatt ccattttcat cgcctattgc aactgaacaa acagatgttc cagtttcaac
    44341 gcaaagtatt tttgccattc taaaaaactg actactcggt tttaaaaagt tcgtctttgt
    44401 ttttaacttt tctgattaac gacttgtcct taagcttacg cgatattgcg ctatagggtg
    44461 cggtaattac ctcctcatcg ccctttagcc cttcaataat ctcaatatac tggttatcct
    44521 gtatgcctgt ctttacttca actattcgag cggtatcgcc tttggttata aaaacaattt
    44581 ccttaggctt ctcaaccttg gttggtttgg attcaacggg ttgggtgtta ttttcaacct
    44641 tttcccattc tcctgttttc tttacagtat cgattcgggt tgttaccgca agtatcggaa
    44701 tggttagtac gttttccttg gtgtttgtaa gaatttcaac agtagtggac attccgggtc
    44761 ggaagggact ggtttgtgaa ttggtgagaa ggtccttata tgatttttca agaataaata
    44821 ttcgcacctc gaagttggta acctgatcgg ttgtaattcc ctgaacatta gcggtatttg
    44881 ctatctgggt tactattcct ttgaatttac gacccaggta tgcatctacc tcaattaatg
    44941 cagtatcggc aatcttaacc tttactatat cgttctcgtt aacgttaacc cttgcctcca
    45001 ttcggtttag gttagcaatc cgcatgattt ccgtaccagc catttgtata gtacccacca
    45061 cacgttcgcc aagctcaacg ttcagctttg atactgttcc cgatattggt gcgtatatgg
    45121 ttgttttgcg aagattttcc tcggcttcgc gcaacgatgc ctgagcgctt tcaacgctat
    45181 aattagcagc ttttagctca gcttgtgcaa cctcgtagtt ggttcgtgca gtttcaaact
    45241 ccgactccga tattgccttt tgttcataaa gctgtttgct tctattgaac gacaactccg
    45301 actgggcaag ttgggcctta gcctgaatta accttgcgtt tgcagagtta acagcggcaa
    45361 ttgctctttc ttttaatgaa atatatgtat cgggtttgat gcggcaaagc agggttccct
    45421 tttcaaccca gttcccttct tttactggta attcaataat ttcgccagaa acctctgagc
    45481 tgatttttac ttcaacctca ggttgaatct taccattggc tgtaatagct tcggtaatag
    45541 ttctgtactc aggtttctca acataaacgg gtgtggttaa gggtttacct atccaaccct
    45601 ttttcttggc aacaactgca aatactatca agatgacaac tgctcccagc aagtatttaa
    45661 taagtccctt tttcattatt taagtattta tggtttattt acttcagttt aacattctct
    45721 ttgtttagca agtaacttgc tttcattact ttaccaaaac tattagctat agcttaagtg
    45781 gcaatccctt gtaaaattcc agtatcttgg ttttaaaaat cagctcatac tttgcctgta
    45841 gcaaatcggt ttcggcttga gctagcttgt ttttggccga tgtaaaatca agagctgtta
    45901 ctagtccaag gttaaaccgg ttctgggtgt acctgaacga ttcctccgaa gcagttttgc
    45961 ttttttgcga tgactgcaat tttttttcgg ctgcaatagc atcggcatag gcctgttgaa
    46021 tgtccttgta taatgcattt cgctcactct ccagcgcaag ctgtgcattc ataagaccaa
    46081 ttttggcggt agttactctg ttacgagcgc ttaaaccatt gaatatgggt atgcttaagc
    46141 ttaagcctac cgatttgctc gcattatttt taatctgagt cataaatggg tcgccgtcaa
    46201 atgcgtaatt atcctgaatc cggtactgtg caccagaatt gtaatttgcc cctaacgata
    46261 accggggttg taaaccacct ttcgctattt ttaggccaag ctctgagctt tgtaacctat
    46321 acctcgcgct ctgtatttga ggcattattc cctcagcaac tccaaatatc tgactgggcg
    46381 attctgatac cagctttacg ctatctaatt gatgtattac aggttttgca atctgaaatc
    46441 cttcggttgt ttttagctca aggagctggg ttaggtttag gtaggacagg tttagctggt
    46501 tttgagcatt tacaagctgt agctcatccg aagccacaag tgcttctacc tcgtagaggt
    46561 ttccttccgg caaactacca gctgaaacta aaacttttgt cctttcaagc tgcattgtgg
    46621 aaagttcaac ctgtctttta gcaatctcaa ccagctcttg ctgataaagg atttgaaggt
    46681 aaagcgatgc tatcgttagt gcaatgttat tcttaacctt ttcaacatcg gcaacagatg
    46741 cctttaggtc gaaatggttc tgcttccggg tgtttgttag ctgaaaacca ttatagatgg
    46801 gcaaacccgt actaactgaa aaactggttg cctgagtatt caaggcttcc ttggtattgg
    46861 taaggtaatt tgttaccaag ccaaagctat aggaatgatt accattgata ttaattgttg
    46921 gcaatgcatc cattttggat tgtaaaaggt ttacctcatt tacctttacg tttagctcct
    46981 gctgcttaac ctggatatta ttagctagtg catactcaat gcaacgctca agtgtccacg
    47041 gttcttgaga aaaaactacc gaggttagga ttaacaaaat gaagatgggg agaatagttt
    47101 ttttcatact taaaacgtta tttaagaacc ttacttatga cgtttatttg cggaattagt
    47161 ttaaacaaaa atcaatactt aaaaaattcc ttttccgaaa gtaggacttt ataaactacc
    47221 atagtaaaaa agaagtgtta aaaaaggttg gctactagct tcatatagct attggttacc
    47281 ttttcctttt aaaaatcggc aattgcgact catcaaattc cgttttacga gggattttta
    47341 ttgtttcaaa atcaaaactt tcagtatcta ctacaaccgc ttgaaatctt ccaagtgagc
    47401 acgtaccaca gtttaaatat atttttttac tgttcaggta gtaaacctta tgcgattcaa
    47461 gcttatgggt atgcccaagt ataaccatat cgtacttttt tagtagctta agggcataca
    47521 tgaggtattt gatagtattg tactttcggg gtattctgtt gacatcatca aagtaatcga
    47581 ccgcgctata aaatttgcgt tcaacccaac ggaaacggac tagccacttt agaaacgagt
    47641 acatggtaac gctaataaat cggccaaagc gtgtaccatt taaaaaatcg gctttgtgtc
    47701 cgtgctcaat gtgtatggtt tttccttctg aattcttaat atggaaatga tcatgtgtat
    47761 gagggaccca aatgtcgtgg ttgccacgga gttgtacaaa accttttcgg ttgaaataat
    47821 caataatctt agcatttacc tccgcaatct ccgcgtagga atacttgtag aggtcaaata
    47881 tatcgccgtt taatattacc ttttcaacac cataatgctc aattacccta tcaagtgtgg
    47941 acataaaggc tttagccttc cacccaaagg tgccaagctt tttacctgtg tcgatatgca
    48001 aatcggaaat tacgagaagt atcacctaaa ctaattttat gctaatttaa gcataaaaat
    48061 caacccataa gcacctcctt aacccgtttc atttcttttt ccaattgctt ggtcgcatgc
    48121 atggtgtatc ctatgaatgg aattaaaact atacgtacgt ttctattttc agcattaaaa
    48181 aaaattattc ctcctgcacc ccaatttttc caggttaaac cgttatgaaa ctttatggcg
    48241 gccctatctt ccgatacctt aacgtatgaa taactgcttg taaatcgctc ctttaactca
    48301 ctaaactctc tgattggggt ttctacttgt tcacttaatc tatgaccgta cgcaggttct
    48361 tcaaatgagt cggactctat ataacctaga gttttagata ggtttaacca tattggtcca
    48421 aaaaagagaa agacaaaaac aaaccctgct atagctgact cgattaatat tccagaaaag
    48481 gatatgctaa actcctggta tcctttaata ataagtttta ccaccaaaat ggctaaaaca
    48541 taaatcagaa aagcaaccac agcgtaatat gaaaagaaac gtttcatctg tataaaatat
    48601 ttatattatt aattataact cgttactttc ttgcttttaa caaacattat cgagcggatg
    48661 gaacacccat agtttgactt cgtcgagctc gcaaaaaacg ctcggttgtt catctgtgtt
    48721 tgtgcaagct gccttacatg ggtgtttcat aaaatttggt ttaagtttga tctgaaaaca
    48781 aaaataactc ctttcactta aaccatcaac caatgcttta tttttattac accagatagt
    48841 actaacttta tatctcaata aaagccataa aacaattacc tttgcacaat aaaggaagaa
    48901 ccagatgaag gaaagtaagg atttagttcg ttggtatgcc gtttacacca agccgcgaaa
    48961 cgagaaaaag gtttacgccc gtttggtaga aaaggggatt gaaactttcc tccctttaca
    49021 gaaaaaacta aaacagtgga gcgatagaaa aaaattggta gatgagcccc ttttccgctc
    49081 atacatattt gtccatattg acttgaaacg gtactatgat gtattaaata cccaaggggt
    49141 agttcggtac ataactttcg aaggcaaagc agtccccatc cccgacaggc aaattgatca
    49201 gattaagcag ctgcttgttc aggatattga gcttgaagcg gtggagggtc aaattgagcc
    49261 gggcacaagg gttgaagtcc gatttggctc gcttcaggga attgaaggcg aaatggttga
    49321 acattcgggt aaaaagaagg tggttatccg gatcgatcat atcagccact cgctcctggt
    49381 tactctgcct gctgagtatg tggtggtgaa aagataataa aaacagggct tgcaccctgt
    49441 ttcactatca aagtaatttt actcaaaaag aaaatccaaa tcatcgccgg ggtaaacctc
    49501 gcggggtggt tgtccttttt tgaaaattcg agcaggtttt gggccaatca ggctaaactg
    49561 cacatcttcc agctttatca tggttccctc gcggagtcct acaacgtata catgtgggtc
    49621 catttcaacg aactccataa ttctttgctc acgggtttcg ccagcatgtc cctcggggtt
    49681 agcatccagg tagtgggggt ttatttgaaa ccgaaccaaa ctgaaagcgc tgaaactgtc
    49741 tggttccgta attggcatat cgttagtggt gcaaatggta ggacaggcaa catttgctcc
    49801 tgcactccaa ccaatgtaag gcgttccact taatacttta ttccttactg cttcaataat
    49861 atcggtctgc tgaatagttt ttagcaactt aaaggtattg cctccgccta caactattgc
    49921 ttcggcctcc tgaaccgcta gctttgcatt ccgatggtga tgtattgagt caacctcaac
    49981 cccaaattca ctgaatcgtt cctgaacctt ttgctcatac tcattgtagc tgaacgtaac
    50041 ggcggcccat ggaataaaaa gaaccctttt aatgctacct aaaaattttt caatctcctt
    50101 cttgggatgc tccaggtaag cctctcctgc attggttgag ttgcttatga gtaatagtcg
    50161 aaccatgatt ttgaattcat tttgtttgtg gtaaaaatac aaaaaaacta ataccatcaa
    50221 accttaaatg cagcaataaa agcatcctcc ttaagctcaa gggactttat aaaattgagg
    50281 gctaaatcct tttgctctgc aaactccatc aggtcaatgc tttttaccac ttttcgtaat
    50341 tcattcatgg ccgatttatc gtccgatgtt ccaaataggt atacaatatc gccgggtcga
    50401 aggttcgacc aatttaaagt gctgcttaca ataaaggaac caaattgtcg aaatgccata
    50461 ttatcggggc aaagagtcaa tatctgcttt ttatagagaa taaaaggata aagtttgatg
    50521 gaatttttac ctaacgactt tacaattgga tcatctttta aatagagatt taatggttca
    50581 agtacacctt cgttcaattc cccattattt tttatgtact caataacaat gttgtatata
    50641 ttgaagttaa tgaaatcgca acaggcatta ggctcggcag caattacata ataacagaag
    50701 gtgtaatttt tatataaaaa atggaaaaag tttggttttt catctacttt tttgattttg
    50761 gcctcccacc aagcagaaaa atatccttca acctcgtagc tgtttttcac catctgcagg
    50821 ttacaaaggt tttgggagat attttggaat aaatggtttc ggagcagctc cagatttact
    50881 aaagcccttt tcttttttct attttcaatt accaccaaca ggaataggct aataaacaca
    50941 acactaaacc ccattgctat aaataggttt aaacgttgag ctttcagctt ggagttcata
    51001 gcgttaattt cctgctcctt tttatccctt tcgtattggg cttgcagttc ttcaattttt
    51061 tgaagggttt cagagtttaa aatggaatcc ttaatggtga tatggtcaag caggtagcta
    51121 tatgcttttt gaaagttatt tgttttaaca taacactcgg agaggaactt gttgcaatca
    51181 ctttcaacat cctttaaacc aatctgctta gccttattca acgattcctg aaacatattt
    51241 attgctcgtt caacttttcc ttgtgcaagc agaacctgcc ccatattttt ttgggtatag
    51301 cataccctgg ttacgctttt aagcgattga aacatttcaa gtgcattctg gtggtaaagc
    51361 aaagcattat ccaaaaggtt ttgtttaaag taaagtgaac cgatattatc ctgaattatt
    51421 gctacaccta ttgatattcc taatgaatca taaatatgaa gcgatttaag aaagtactcc
    51481 aaggcatcat cgtactttcc aagttccaag tatgcactag ctatattatt cgatgcccca
    51541 gctactgctc tggaattgcc ctttgcctcg ttaatttcca agtattcctt gaaaaattct
    51601 attgcttttg gatagttttc cctgtaaaag tgaatttgcc caatactatt cgaagcaatt
    51661 ccagttccct caatatcacc aatttttcta tataaatcca atgcatttaa gtagaactca
    51721 agggctttat cgtatttgcc ccaattacgg taaataagac ctatgtcgtt ttgtatgccg
    51781 gctaataacc taacgctgtc gaggtatttg tatatagttc ctgcgttttc caaattttca
    51841 agcgacaacc gaaactcacc cattttcatg tagctaaagc caatttgatg atacgcattg
    51901 gctacaagct ccctttttcc caacctgttc gctaggctta aagcactttt ccagtaatct
    51961 aaacttttct cgtaattgct atcggaatag gctatggcta gctcggttag cgttttgtac
    52021 ctattcaaat ccgattcttg cttttgtaac gcttggttaa gactatcaat agaatgattc
    52081 tgtgccaaac cattattgaa tagaatggca aaggtcagta ttgccgatat gatgctaatt
    52141 cttaacacta aaatcaattt atagcaatat acggaaaaaa tgaaaacaag taaacagttg
    52201 tattaaaaat gatttaataa acgttgatgt ttttgggagc taaagaatag caaaaagtaa
    52261 taagtgttac ctaatagctt aactaggtag aaaaatagtt accaatgttg tttagttaag
    52321 gttatttgtc atgttgcgcg ctagcgaaac attatgctgt cgttctgagt cacgatttat
    52381 cgggataaac ttagctgaag catctcattg ctcatttttc ttcaatcctt agaaaagatc
    52441 actc
//
LOCUS       DAOOWJ010000004        48774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_781_length_48774_cov_9.431084, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000004 DAOOWJ010000000
VERSION     DAOOWJ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 48774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48774
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_781_length_48774_cov_9.431084"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLW22_00845"
     CDS             complement(<1..229)
                     /locus_tag="PLW22_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73276.1"
                     /translation="MSDAILKIERTRFKISLQIAFLFILLIWLVKLVEIIEGTSLYRL
                     GLQPRSFSGLVGIFTAPLIHSDFNHLINNTIT"
     gene            complement(222..1241)
                     /locus_tag="PLW22_00850"
     CDS             complement(222..1241)
                     /locus_tag="PLW22_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73277.1"
                     /translation="MSRIRKITQIFILCIVTISSYAQGNLFEEQSILEIEIIGDLKEL
                     FYDVNPQNARYHDIIIYYLYADTSVSLPAKAMTRGIFRRDKSNCKIPPLAIKPQKIND
                     PIFNTSQRIKLVNPCMNTKRAQQHIIKEYYAYRIYNILSDYSFKVRLVRLIYLDRFGK
                     YKNFITYGILIEPVKWLAKRTKTKEIETQGIIQNATYRPVLDVMTMFQYLIGNTDWSV
                     PKLHNIKLLYHDSLTSVIPVPYDFDFCGFVTPPYTKPPEIIPITNVRERYYRGNCRQK
                     LELNFTIDFFIKKKPEIMNLLTADSLLTVRNKNDILEYIDMFYNTLSDPKLVKREFID
                     NCRNE"
     gene            complement(1238..2875)
                     /locus_tag="PLW22_00855"
     CDS             complement(1238..2875)
                     /locus_tag="PLW22_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU73278.1"
                     /translation="MKFKLALHLLVFTPFLFVHGFAQDNTSNDSLVVSFINENNISKI
                     DSLIKVNGINKTYFNNSHTLLTLAISNSNLNLIKNLVDRGANVNIVLNTLSPLIQCAI
                     YDKDTIAEYLLTHGAIVDVYNIQRNTPLLYAARVGSVNTLKVLMRFRANPFFQNFQNY
                     NSLEYALSFGKTEAANLLRDYMVAYSKGIYPSCFDGPHVERISKRRARVFYLVNDSIN
                     SKVFVKSKTFRATNGVISFKGFYATDSINYTVNFNKPSKYSENETVINSKVKIFAVGD
                     VHGEYDSLAKLLVNNSIVDKNLNWIFGDGHLVFIGDLVDRGERVTDVLWLVYRLTEQA
                     EQSGGRVYTLMGNHETIILNNDNRYIHEKYQILTRGNKISYSDLFSKNVWPGAMVRNF
                     KAGIVIDSILFVHAGISYQLAQKNIPLVRMNQLLDKLYNPDNSFLIDPDVFLNELNLI
                     FSEYGLLWYRGYLLQTPTIPKATMSEVSYVLKTFGASQMVIGHTEVDSIAPLYSGMLF
                     PINVPFARRGIVPQGLLIDETRKFWSCSINGQCTLIK"
     gene            complement(2881..5100)
                     /locus_tag="PLW22_00860"
     CDS             complement(2881..5100)
                     /locus_tag="PLW22_00860"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73279.1"
                     /translation="MMFKKILLPVYAILCLHLVAQPQNADSLLLQLYQESNDSLRVLI
                     LQKIARVYEVNENNPVLAENYLLEAKRLAESIKSNYLRMEVYTSLGVLYRNISNYANA
                     LKFHNDALDLAREIDDKHQQAKSLNNLGVVYRRLDNHAYASEYHLKALKLAEEIKDTF
                     NISVACNSLGNIFSLNGRYDEALEYFQRALYLSQQTNNLLGQAMNFNNIGEVYEFKGD
                     FIKAKDYYSKSLDINYQINSQKGIAINFNALGKIELYLGNPLKAQDYFTKALVIDKKL
                     NDKKFIADSYINLARAQLGLNNDRSAYENVFACLSIAKEIKSLIHFQQAYEVLSDYYQ
                     KQNEYSKALEYFKLSSSYKDSVLNEKNSRHISTIQTLYETEKKENEIKLLLQQQELKQ
                     REITRTKIQTYVLLIGLLLSGAMLLVIYFALRDKRRTAEKLARQLEEIELKNVLLEEK
                     NQEIEQQKQEIEKNKDFIEQKNKNLEEAYNIIESYIQKITDSIRYAERIQESIQPPIS
                     FVKKQFSDTFIFSRPKDIVSGDFYWMLPKGNKVFFALADCTGHGVPGAFMSIIGIDLI
                     NQAVNQHMLYKTDDILSFIDDQLIKRLSKSKNEMILKDSMDIAVCVFDKETYLLEYTS
                     ALIPLFLQRNGDVQELKPDFVTLGAKLAGASSKNFTVKSIKLTPGDWLYLTSDGYFDQ
                     LGGDNHKKFMRSRFKEQLVLTSGMDGPSKCTHFEKTFLSWMKRNEQIDDVLLWGIKF"
     gene            5178..7571
                     /locus_tag="PLW22_00865"
     CDS             5178..7571
                     /locus_tag="PLW22_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOU73280.1"
                     /translation="MAILVKIYHRIFRIIILLLLLMLILVLTGAGILFFKQEYIINTL
                     RENLNKYPDLSLTYSSVSVNTLKTFPRFSYSFTDFVLVINLNSKSDTVLKTDDFLISI
                     SPFKLLGGKIDIKNIRVSNARIKWQTNYPEELFGSSDSSSESSVSVLISGISLNNFFF
                     ELLDSVSKTVLYCSGKNLNIKFKTEHTKLLLSIQSELSKTTVGEYFKITNQHQLNFDI
                     ENSDNKLYIYNLESSIKSLRINGSGIYDFNSQLAAFRFKSNRFQAQDLSYLPYLKSFD
                     QIKGIITADAYLRLSSGFEKVDTFQVNYNSNKLKWITKNEETTITNLEGYTLLTNNFS
                     KHFSYIPKASIESNNSKINLNAKIKGFNNLVILAKGYVSHNLRIQKPEIDIETTGQFK
                     ALISYAPKTESITPLLLKSDLVFENKKNISFNQPSVKGTIAINNDLNIAGYFKSDSTD
                     INFNINQANFLESYTQNRYSPNIYLYGNHIDYNDIVHLITDRTSNTSTKGVDLPKMSF
                     MLNFKRATYNRLKLNSLKANGIINGDTISADYFTADCFDGNVSGRFKSHSNSVHTNLW
                     VSNVSIENVFKNFDNWGQSYVTADNLSGRFKGIMNIQFKRNEKGDVEMGSLKLFSDVQ
                     VVQGKLKGMDRIKELSKWLNLDQVKVIAFDTLKNKITIENRKIFIPNMDIKSNVLLMN
                     VSGVHDFDNRYEYIARINISNLLKRKFVKSDQIDFHSTTDGSINLYLKLFGQNDSYEV
                     EWINKKGFESGIHNTVQTDSVSIQNSPQIKPKEKTDGNVGVGYMLEWDEQIDTLKNE"
     gene            7573..8721
                     /locus_tag="PLW22_00870"
     CDS             7573..8721
                     /locus_tag="PLW22_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU73281.1"
                     /translation="MKILSPRNKFLLTLIIAAVTVSFITIYFTNRFVSELKQEERQKI
                     DLWANAVKYISNEMGSCEDFSLIFEIVQNNRTVPVILTDETGKVIGFRNIELPDIQDT
                     VYVSNLLNKMRAEHDPITIELPDGSKNYVYYFDSITLKKLNYYPYIQLIIIAFFIGIA
                     FYSYGQARKSEQNLIWLGMAKETAHQLGTPTSSLMACSSLLSEKYPNDEVVTELEKDV
                     KRIETITSRFSKIGSSPSLDSIDITNLITDSINYQSKRCPKTIAFNFSPGIPIYAKAN
                     HILLGWVLENILKNAVDAMQGKGVISVEATVEKNNVIIDIQDQGKGIEKGKQKEIFQA
                     GYTTKERGWGLGLSLAKRIVEQYHSGKIFVFRSELGVGSTFRIIIKKA"
     gene            complement(8718..8978)
                     /gene="yidD"
                     /locus_tag="PLW22_00875"
     CDS             complement(8718..8978)
                     /gene="yidD"
                     /locus_tag="PLW22_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOU73282.1"
                     /translation="MNLVKTLKILWKYAVRLLAYIPILLIKIYQIFLSPYLPNSCRFT
                     PTCSAYSVEALKKHGFFKGLWLSVRRISRCHPWGGHGYDPVP"
     gene            complement(8980..9360)
                     /locus_tag="PLW22_00880"
     CDS             complement(8980..9360)
                     /locus_tag="PLW22_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOU73283.1"
                     /translation="MPQKLRKKYIIHLENEVNSLLKSELSRFHYPIKLIYQVDERIPG
                     GIEENPHYKVIFIIPKRYLKKAYMRNLAKRRLREAFRINQNLLDQFRERGKTVYLAFI
                     YVSAEVVSFKQLESGVVKLLTDLK"
     gene            complement(9397..10146)
                     /locus_tag="PLW22_00885"
     CDS             complement(9397..10146)
                     /locus_tag="PLW22_00885"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOU73284.1"
                     /translation="MKIKKIVISQPDPQSEKSPYNELSEKYGVHLDFRPFIHVEGVSS
                     KEFRQQKINILDHSAVIFTSKTGIDHFFRICEELRITVPETMKYFCITESIAHYLQKY
                     IVYRKRKIFFGDNTFKDLLDIILKHKDEKFLLPLSDPHKPEIPKALTKAKINYTKGIF
                     YKTVSSDLSDLKPFPYDMIVFYSPNGIKSLFDNFPDFQQNDIAIGAFGDTTAKAVKDA
                     GLRLDVKAPTDAFKSMTQALDHFLKEQSKKK"
     gene            complement(11040..12836)
                     /gene="uvrC"
                     /locus_tag="PLW22_00890"
     CDS             complement(11040..12836)
                     /gene="uvrC"
                     /locus_tag="PLW22_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOU73285.1"
                     /translation="MENLSSISYLSSLPDLPGVYQFFDSTGKIIYIGKAKNLRKRVSS
                     YFNRSQYDSAKVRALVSRVADLKYIVVNSESDALILENILIKKHQPKYNILLRDDKTY
                     PWIVIKNEPFPRVTTTRRYENDGSIYYGPYTSGRLLKALLDLIRQLFPIRTCELNLTQ
                     SNIASGKFKACLEYQIGNCLAPCIGNQKENDYLSNINAIKKILEGDLNEVLRLTTAEM
                     TKAAQELKFEQAALLKERLRLLQNFQAKSSVISKGINTADIFTIQESEGFAVVNYLKV
                     NSGVVVQSYNLQIKNIVDEPIDDIFSQAILEIKERLNLKSTTFLVNINPAYQLSNIKY
                     HIPKQGEKLKLLKLSLRNADAYIKELIKSEERANPQTRALKYLEQLKKDLQLPELPMV
                     IECFDNSNLQGTNPVSACVVFKNGKPAKSEYRHFNVKTVTGPDDFASMREVVYRRYKR
                     LIEEKKNLPNLIVIDGGKGQLSSAYETLKDLHIEHIPIIGIAKRLEEIYRPGDTTPLY
                     LDKRSMSLKLIQNIRDEAHRFGITFHRKKREKKNEASILESIPGVGKKSVIKLYTRFK
                     SLENIKMASLEELSEEVGAKTASNIVSFFKKI"
     gene            complement(12842..14455)
                     /gene="ade"
                     /locus_tag="PLW22_00895"
     CDS             complement(12842..14455)
                     /gene="ade"
                     /locus_tag="PLW22_00895"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOU73286.1"
                     /translation="MKNSNSISGKLVDPIGRRTVNAKIYFQDGVITQIDESLDAENVY
                     IIPGFVDSHVHIESSLLVPTRFAQAAIKNGTVAVVADPHEVANVAGIEGVRFMIENAR
                     QSKLKFFFGAPSCVPASPVDECFEPFTSREVAKLLDLPEVTHLAEMMNFPGVINGNTE
                     VHSILSEAKARNKPIDGHAPGLSGEKLTKYVSAGITTDHECFTLEEAKKKIELGMKVL
                     IREGSAAKNFDALHPIIGQNPSMAMFCTDDCHPDELMGGHINKHVRKAIALGYDIFDV
                     LQVASVNPVNHYRLNVGLLQVGQPADFIVVDNLNDFNVLSTYIKGVNVTNFEEDYAVK
                     PPLFSFPNHFDHQKIKLDFPSGKPVKVIKVIEGELITETLKTSVPGSGYDFDKDILKI
                     VVLSRYNPNNIGVGLINGFGMRKGAIAASIAHDSHHIVAIGANDDSIVRAIDYIIKNR
                     GGMCYYDGNETMGLALPFFGLMTNVDVSDAAKEYSLINKKLKADGCKLHAPFMTMAFM
                     SLSVIPHLKLTPAGLFDVDRFSLTSIFAE"
     gene            complement(14603..16459)
                     /gene="mnmG"
                     /locus_tag="PLW22_00900"
     CDS             complement(14603..16459)
                     /gene="mnmG"
                     /locus_tag="PLW22_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOU73287.1"
                     /translation="MELAFDVVVVGGGHAGCEAAAIAAKMGSRTLLVSMDLTKLAQMS
                     CNPAIGGIAKGQIVREIDALGGYTAKVTDLTTLHFRMLNQSKGPAMWSPRAQADRMLF
                     SIEWRKYLESIPNLYLWQDEVIDLIVNENRVEGIITKTNQTVKCKTVVLTNGTFLNGK
                     IHVGNVNFTGGRIGELNSQGLTERLVKLGFTTGRMKTGTPVRIDGRTVNYSKLTIQES
                     QELSKFSFNPDYFSKLPRKHCYIVNTNQVVHNILREGFDQSPLFTGKIKGIGPRYCPS
                     IEDKVHTFAEKTSHQLFLEPEGVFTYEMYLNGFSSSLPVDIQYKALKSIPGFENVHIL
                     RPGYAIEYDYFDPTQLKHTLETKIVQNLFFAGQINGTTGYEEAAGQGLIAGANAHLRA
                     NDIQENFQLSRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILIRQDNADERLTPIA
                     AKYNMVDENDVKRLENKIKSIEELNQLVKEYSVSPSDINEYLTSNNSAKITQKRKLYD
                     IVLRPEISLKPTLEHLKINIDSYDYEAINGAEIKIKYSGYIEKEKIIAEKINRLEHIK
                     IPDDFDFNQLNSLSTEARIKLTSIKPKTIAQAKRIPGISPSDINVLLVYFGR"
     gene            complement(16450..16887)
                     /gene="ybeY"
                     /locus_tag="PLW22_00905"
     CDS             complement(16450..16887)
                     /gene="ybeY"
                     /locus_tag="PLW22_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOU73288.1"
                     /translation="MAIFFSYKTPSISNRISNKNKIKKWIIYIIEKEFKKSVGDINFY
                     FTDDTEILEINKKYLAHNYYTDIITFNYCTENSISGDIVISVDTVKTNAKDYNVTFEN
                     ELHRVIVHGILHLLGFDDSTEEQKKEIREKENWALKIFYSKWN"
     gene            complement(16887..17282)
                     /locus_tag="PLW22_00910"
     CDS             complement(16887..17282)
                     /locus_tag="PLW22_00910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73289.1"
                     /translation="MERTITIPSKLEQISLIEKTIDEITEKLTLTPDVYGKILIAAIE
                     GVNNAIVHGNKLDEKKVVKVNIKICDTKVCITISDQGKGFDPNTIPDPTIPENIENIS
                     GRGVFLMKKLSDEIAFENNGTTVKMTFNI"
     gene            complement(17358..18542)
                     /locus_tag="PLW22_00915"
     CDS             complement(17358..18542)
                     /locus_tag="PLW22_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU73290.1"
                     /translation="MKVIINLVLVFLFSVNFTFSQNKIDILNNQKKEILKQIDDNNKQ
                     IDIINKSKKATINELTLKLKSIELQNRLLINIKNQITEISKNLTETTSQVDSLSTLID
                     TRRNELIRIYKSYFKKLKRKNDLLILLLSSSSINQAYTRLKMYKNLVNYFNQQISLLN
                     ENKIELEQKRSNYVLFLKELKLKENEYQKTITTLNKEKNELEATKKDLEKKKIELTKE
                     ISNKKKALALIDNEIKRIIEENARALKSMNKENAKRYNELSNIFKENKGKLPPPALNS
                     NVLNYFGESNHPILKNVKVKNNGIDLILNNSSTVQAIHRGEVKKIFNVPYGGRAIIIR
                     HGEYLSVYSNLSEVSVKVGQLVNAGDNLGSVMKQTDNTLVLHFEIWNEKEPENPLDWL
                     KF"
     gene            complement(18542..18979)
                     /gene="dut"
                     /locus_tag="PLW22_00920"
     CDS             complement(18542..18979)
                     /gene="dut"
                     /locus_tag="PLW22_00920"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOU73291.1"
                     /translation="MEIVKILNKSTFDNPTYATEHSSGVDLKAFIEEPINLKPLERKL
                     IPTGLFIELPVGIEAQIRPRSGMALKKGLSILNTPGTIDSDYRGEIGVIVVNLSNDIV
                     TIEPGERICQMVFTRYEKVSFENVNKISETDRGAGGFGSTGHK"
     gene            complement(18970..20442)
                     /locus_tag="PLW22_00925"
     CDS             complement(18970..20442)
                     /locus_tag="PLW22_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU73292.1"
                     /translation="MTFILGKMSSVKQLYSQSIIYGLSTIVPRLLNYLLVPIHTNVLT
                     DPRQYGVITEFYAYISFLLIFLTFGLETGYFRFVNKYKNDERVYSNAFTFVLTLSSIF
                     FIAAYLFSDSISSLLGSGYLPIYIITTAAIVSLDSITALPFAKLRNQNRPLVFSFIKI
                     TGVAINVLLNIIFYLVLKPSYLKNLFPHIDLLFYVFLSNFIQNIVVFVLVTYFTGFPK
                     IRIDKSLMKELIWYSFPILLAGLSGTTNEAFDRLFIKYLLPKSVDSLYQLGIYGACVK
                     LAVLLVLFVQMYRFAAEPFFFKSSGSQDHNKLFGKSFKYFLIFSIVISLLITFNLPIL
                     KFFIGKNYRESLYIVPILLLANIFYGLFFNLSFWYKLADKTIYGLKYTVIGSIITIIS
                     NIILIPIIGIYGAALSRLITYVTMTIMSYSDGRTSGFVSFETKNIKFYIIFILFITLS
                     GTLLLVFLKPYFSLIAANILIGLFIYKFIKKENIKITLWR"
     gene            20499..21296
                     /locus_tag="PLW22_00930"
     CDS             20499..21296
                     /locus_tag="PLW22_00930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU73293.1"
                     /translation="MKIRTLVLLGLVSMLSCTTNYNDNNRYVKISTDVGDIVIKLYNE
                     TPKHTENFIKLTEENFFDGLIFHRVIKDFVVQGGDPETKNAEPGKLYGEKDAGYLIDA
                     EIVDTIYHKRGVIAMAREGDDVNPEKKSSSSQFYLVVGKVFTDEELNKLEDKLNKKLR
                     EKTEKKLYDSLLGSQSNLNPKFLNQVTQKVSILADSIMNVKHIKFNENQRKIYTTVGG
                     IPHLDGNYTVFGEIVEGYDVVEKISQVQTDSNDRPLKDIKFKVRIVK"
     gene            21305..22324
                     /gene="pheS"
                     /locus_tag="PLW22_00935"
     CDS             21305..22324
                     /gene="pheS"
                     /locus_tag="PLW22_00935"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOU73294.1"
                     /translation="MEERIKQIRETFSTLDPKDLQSVEKIRVEYLGRNGYLTKLFDDF
                     KNIPNEKKKEVGKILNQLKSEISDKVETWKKTFSESTKSKGTNLDLSLEEYPFEFGTR
                     HPISLVKNELVEIFKYLGFTVYEGPEIEDDWHVFSALNFPEEHPARDMQDTFFIEKKP
                     DILLRTHTSSVQVRSMENEKLPIRVICPGRVFRNEAISARAHCIFHQIEGLYIDKDVS
                     FADLKQTLLYFAKEMFGSETKIRLRPSYFPFTEPSAEMDVSCKLCGGKGCSVCKYTGW
                     LEVLGCGMVDPNVLELNGIDSKIYSGFAFGMGLERIAMLKYQVRDLRLYFENDVRFLK
                     QFKNY"
     gene            22327..23025
                     /locus_tag="PLW22_00940"
     CDS             22327..23025
                     /locus_tag="PLW22_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU73295.1"
                     /translation="MLKDGNSNTCISSSFPIFKSLNSEELELLNANLKCNTYRKGEII
                     YREGSRITGIYCVKKGILKIYKTGIDSKPQIVAFAIKGDITGYRSVLSNEMACTTAEI
                     IEEAEICFIPSEIIFNLIKKNSDFALSLIQLTCRELDQANIFIKDIAQKTVKQRLAEI
                     LLMLENTFKLDAEGYIAVNLTREDLSSIVGTATESVIRILGEFKNEGLIAIKGKKIKI
                     NDKKKMNLISDSIF"
     gene            complement(23022..24020)
                     /gene="ruvB"
                     /locus_tag="PLW22_00945"
     CDS             complement(23022..24020)
                     /gene="ruvB"
                     /locus_tag="PLW22_00945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOU73296.1"
                     /translation="MDSKIIIDKEFEKHIRPVEFNQFNGQNDIVKNLKIFVKAAKLRN
                     EALDHVLLHGPPGLGKTTLANIIANELGVSIKTTSGPVLEKPGDLAGLLTNLEPHDIL
                     FIDEIHRLSPVVEEYLYSAMEDYKIDIIIDKGPSARSIQIELNPFTLIGATTRSGLLT
                     SPLRARFGIKFLLEYYTVDILAGIVKRSASILKIDIRDDAAVEIAMRSRGTPRIANAL
                     LRRVRDFAQVIGNGYIDIDIAKEALNALKIDSRGLDEMDNKILLTIIEKFKGGPVGLN
                     TIATAVGEDAGTIEEVYEPFLIQEGFLKRTPRGREVTDLAYKHLNKLRFPEQGKLF"
     gene            complement(24020..25003)
                     /gene="nadA"
                     /locus_tag="PLW22_00950"
     CDS             complement(24020..25003)
                     /gene="nadA"
                     /locus_tag="PLW22_00950"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOU73297.1"
                     /translation="MINKESVDKLGYINEPIDSGINLVNEINRLKVEKNAVILAHYYQ
                     SPEIQDIADFVGDSLALAVKATTLKSKLIIFAGVKFMGETTKILLPDAKIVLPDMNAG
                     CSLADSCNYNDFKSFKEKYPEHIVITYVNTNADIKTLSDICCTSSNAEKVIESIPIEK
                     PIIFAPDRNLGNYLKNKLKRENMIIWDGFCHVHQRFSLERLIELKKQNPNAKIIAHPE
                     CQKTILIMADYIGSTSQLIDFTINDPSTSYLVATEYGVIHQMKKFSPEKDFIPVPPDN
                     STCACNDCIYMKMITLKKLYLSLKYEYPVVDLSPEIITKAKLPIERMIAIR"
     gene            25447..26544
                     /locus_tag="PLW22_00955"
     CDS             25447..26544
                     /locus_tag="PLW22_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU73298.1"
                     /translation="MKSNRLGMFVFMAIGLICGHSGWGSNTDTLCVDKKSNDNNYFVV
                     VPVAFYGEETNWGGGLSGGYYFRCRDNKVSSVQGTAVYTLKNQASLWISPRIYTTDRE
                     HYYSGHIRVNHFPNKFWGVGRNTPDTLEEKYVSDDYSVLIQHQRILFGVIMAGVQGQL
                     NYYRTGDYPGDRLLATGISGVNERLTTGLGVLLTWENRENLYYPSFGEFYKASLMVYS
                     KIFGSELNFTKLTIDLRNYYNIYDDHVFALQFLADMTWGKVPFQLMPMIGGADVMRGY
                     YQGRYRDNAMACLQGEYRFPIYKWLKGTIFGGLGDVAPYFDKLDISKSKLAYGAGIRV
                     RVNPIKVNLRFDAAYSDRKEMAYYFTVTEAF"
     gene            complement(26599..27987)
                     /locus_tag="PLW22_00960"
     CDS             complement(26599..27987)
                     /locus_tag="PLW22_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOU73299.1"
                     /translation="MFEKQVYVERRLKLRKKIKSGLVIILGNTESPMNYKSNTYRFRQ
                     DSNFLYFFGLDIPGLVGVIDIEDGKDCLYGNDFDIDDIIWMGPQPTLKELGEKVGILS
                     THPFKELYSTLSRAIKHGRRIHFLPPYRADNVLLLEQLLGINQSVIKNYSSVELIKAI
                     VSLRSVKEDVEIAEIEKACEVGYQMHVTAMRMAKPGISEQAITGAIEGVAIAQGYMPS
                     FPIILSQNGETLHNHDHSQMLTSGRLLLVDSGAEISSHYASDFTRTIPVDGKFTVKQK
                     EIYNIVLAANNKAIEISKPGIPYMDVHKAAARVIAEGLTKLGLMKGNPDDAVANGAHA
                     LFFPHGLGHMMGLDVHDMEDLGENYVGYDDEITRADQFGIAYLRLGRRLQKGFVLTVE
                     PGIYFIPALIQKWKSEKINESFINYAKVEEYIGFGGIRIEDDILITDNGSRILGKRIP
                     VTVEEIEALMKH"
     gene            28181..28909
                     /locus_tag="PLW22_00965"
     CDS             28181..28909
                     /locus_tag="PLW22_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HOU73300.1"
                     /translation="MIVSHRHRFIFIKSAKTASTSVELMLSKICGLDDVLTPLAPFDE
                     RFDADYYEYQPRNCEGFQNHMSAIAIKNKVGEEVWNQYLKITIVRNPWDMVVSRYHWE
                     KARLWPSIGKNVANILREPFAIVRYKRLLHRIKLIFVLSSFSNFIRYFDKTWTNSRFY
                     FDEEGKPICDVYLRFENLNKGINDLEKMLGIQLGDLPRLKSKPRKGKKHYSQYFNQQQ
                     KERVEKLFKHEIEVFGYTFEQVNQ"
     gene            28916..29314
                     /locus_tag="PLW22_00970"
     CDS             28916..29314
                     /locus_tag="PLW22_00970"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOU73301.1"
                     /translation="MLAKTYQLIVKYKNSLMWALVILILCGMPPSGLPTIKIPGIDKM
                     VHFGLFAVFALLLISEMNYQRVQLTVKNRHKWVGFIVASVYGGLIELTQQYLFTSRGA
                     EWADFFADILGAAVAVLVYPYINKTLKGYI"
     gene            complement(29377..30261)
                     /locus_tag="PLW22_00975"
     CDS             complement(29377..30261)
                     /locus_tag="PLW22_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73302.1"
                     /translation="MESTKSSKAVSFLSVLIVILLVVIAGLSYVYYQQKKEAKETETM
                     LLAEKDSLSNNLLNLMNDYKDLETDNDSLNRKLQMEQERAQKLYSELQNLKRVSYAKI
                     KEYQKELGTLRAIMKNMVREIDSLNTLNQQLIAENIKVKEEAATAKKTVKELEQRTEE
                     LNTQVAKGSVVKARNVTAVALNRRGSETSRASRTAKIKTCFTLNENAIAKPGSRYVYM
                     RVVGPDDFVLAKSETDLFDFEGEKIVFSANREVDYQNKDVDMCIFFDSSGELAKGTYK
                     ITLYMDGYMIGYTELVLK"
     gene            complement(30292..31221)
                     /gene="gldA"
                     /locus_tag="PLW22_00980"
     CDS             complement(30292..31221)
                     /gene="gldA"
                     /locus_tag="PLW22_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOU73303.1"
                     /translation="MSITVNSITKQFGKQVALNSVTFQVPQGQVVGLLGPNGAGKSTL
                     MKIITGYLTPDSGSALVNGVDINSPAYDFRRDIGYLPEHNPLYTDMYVKEYLLWVAGI
                     YRLGKNARKRVLEIIDITGIGHEQNKKIGSLSKGYRQRVGLAQALIHNPSVLILDEPT
                     SGLDPNQIVEIRNLIAAVGQNKTVILSTHIMQEVEAICGRIIIINKGSIVADGPASEI
                     KALTSSSVQNVVVEFLEAPSVELLQSIENVSNVNQLTATTFLVSSSSNTDIRPTIFNF
                     AKNNNLTLLTLQQKVLNLEDVFQNLTLTEPNNR"
     gene            31446..32663
                     /locus_tag="PLW22_00985"
     CDS             31446..32663
                     /locus_tag="PLW22_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOU73304.1"
                     /translation="MIFESYAVAMVNPKTIDMKKFSSFIIAVLAGTVTHAQSASESIN
                     TFAFDLYRGLKVGGNENLVYSPFSITPAFGMVTLGARGETLNQLNATFHFGNSPKFHQ
                     AVGKLQQSLIKSSSADITINVANKAWLQTDYKILKRYRCDLKGNYKTSLYKVDFINDP
                     EAARKTINHAVEYDTQRHIKDLLPQGSISNLTRLVLTNAVYFKGKWKETFEPEKTKER
                     DFTLADGSKTKHQFMSADKTFGYLKGTDFAALEMDYKGEELSMLLILPNEGKPLSEFE
                     KAFTVEKYNEVVKAIEPQKTLVFIPRFTVESGFSMKKVLGEMGLTIPFSDDADFSGIS
                     GNKDLKISDAYHKAFIEVSEEGTTAAAATAVVVAMKSMPNFNVFDANRPFMFILRHKP
                     TSTILFIGRVAKP"
     gene            complement(32989..33345)
                     /locus_tag="PLW22_00990"
     CDS             complement(32989..33345)
                     /locus_tag="PLW22_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOU73305.1"
                     /translation="MDKDKRTYLMYLEDIQLSISRISEYIAGMDFISFKRDYKTIDAV
                     IRNFEIIGEASKNLPDFVKEKYPKIPWREMYYLRNKVSHEYFGIDYEIIWDIATNYLP
                     ENKLLIEQIILEESRK"
     gene            complement(33347..33637)
                     /locus_tag="PLW22_00995"
     CDS             complement(33347..33637)
                     /locus_tag="PLW22_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU73306.1"
                     /translation="MLNRDDILNKLRELKPFLKREYSVKEIGLFGSFASDSARDDSDI
                     DLLIELEKPIGWKFFSLEIYLEQIFGRKIDLVTKNALRDRLKEDILNKVNYV"
     gene            complement(35290..37572)
                     /locus_tag="PLW22_01000"
     CDS             complement(35290..37572)
                     /locus_tag="PLW22_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73307.1"
                     /translation="MNTKSIAFIGLLWLSALLAFGQRDIEPRLLFSDTASFNFTGEWQ
                     YLTTDIFLLNGDKFSTLINELDFARVEPKKKRRKKLGVEEEQLEYLFITASLKNVKFF
                     GDNDITYPLYNFQISRDKDSKYQTFVSDNIDYVRIIDNLPLYSARDYIDAEIRVRAIT
                     NNDRDQMLALVASQLKNLSKITTPTDAVMSIIGEFGNFIEANTKKKEYRFSSTIRLFE
                     QKNFDTRLHSIRLYLLSTANTPALEFSGVPLRQFLDTVTNGRVNRNQLRELIGIRSYP
                     VIVVANYKSLYRTEQISGDEVTFANIEKRKLKVENDFRQGLINAETYRQEKDLLNFLN
                     IFAQLKNHLDIYSLNYRTGNNDAISVSLFRVMQYYRQLQKAYEEMKYKYRGNNTFSTV
                     FNQEYESILGFASLYMDNDHNLKSTKNLVNTLVRLEADPKVPNADLEKYIADLRFSDI
                     FKPELMSQNLEGQIIANHLSRLEEQLFKGTFEAEIERVRKAEANPKNKTITDNLLRLA
                     KSTSCSLCRERSLGAVTEFTQRLDAYYLKVELQRYDSIATALQPWVFNRIELIQLMQQ
                     NFKHLYPDNANLESARFLNGKITEIERDVFNIRDFLKIDLSGKDLSMVKNFNEKLLAI
                     QRQADANIDLICKLRPELCSRQQAATQNMKNTELSNLFTRSDSVARQTEVFYSIFQFQ
                     LNRLARVPETSKNDELRIETERLAHQLDELKVAISLLDGKNITQETYSKLVKQVNQQV
                     KDISDKLVTLNEKVSLSSNP"
     gene            complement(37780..37932)
                     /locus_tag="PLW22_01005"
     CDS             complement(37780..37932)
                     /locus_tag="PLW22_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73308.1"
                     /translation="MSYNKRQVFEDNLEAIRTYYDITGNEREKTKEDIEKISKYKGFG
                     GLKVVL"
     gene            complement(37936..38364)
                     /locus_tag="PLW22_01010"
     CDS             complement(37936..38364)
                     /locus_tag="PLW22_01010"
                     /inference="COORDINATES: protein motif:HMM:NF020558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1896 family protein"
                     /protein_id="HOU73309.1"
                     /translation="METKENFRKYLDEYMVDFFPEIKENMGIEYEDFLRAKTEMAFRL
                     YNDMIERGEMPLMAELGAIEVLKEGYTNSKYSYIEERIREMDEDYYERLESEGTLKKK
                     IIEIVKAGEGIFEKYGFEKIDFAYTDEFEDEMMEVIKLKL"
     gene            complement(38432..39607)
                     /locus_tag="PLW22_01015"
     CDS             complement(38432..39607)
                     /locus_tag="PLW22_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73310.1"
                     /translation="MANFKLVEVNSKHTRNDFLELPLSLYSDYPNWIRPLDMDIENVF
                     NPEVNKHFRHGEAIRWVLYDNGKPVGRIAAFYDKEIAKNSEQPTGGIGFFECINSQEA
                     ANLMFDTAREWLKSKGMEAMDGPINFGDRDRWWGLLVDGDFPPNYCMDYHLPYYRNLF
                     ENYGFKVYFHQYTYHRLVNSENVDPVVWEKAERIARNPSYTITTISKKNLKKFAEDFR
                     TIYNNAWGRYTGVKKITEAHAMALMKTMKPIIDPELMYFAYYEGEPIGFFIMVPDLNQ
                     VVRHLNGKFDILSKLYFLYLLKVKKVCTKAIGLIFGIVPKHQGKGVEGALVNEFAKRA
                     LAPGFRYTELELNWIGDFNPTMRRVAEQIGARVRKTHITYRYMFDPTKEVVPPRRVS"
     gene            complement(39613..40296)
                     /locus_tag="PLW22_01020"
     CDS             complement(39613..40296)
                     /locus_tag="PLW22_01020"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOU73311.1"
                     /translation="MSTNRFAVIVAGGSGQRMGSEAPKQFLILNGKPILMHTIELFLS
                     LPQKPGIVLVLPKNQMERWKNLCVEYNFTANLTLTAGGETRFQSVKNGLSLIPLNNSL
                     VAIHDGVRPLVSKQVIEECYATAAQKGIAIPVIKPVESVRLVEGSTSKAFDRNSVLLV
                     QTPQVFKSELIKECYNVPEQPYFTDDASVVESQGYTIHTVEGNRENIKITTPFDLQIA
                     EMLIRSKPI"
     gene            40917..42575
                     /locus_tag="PLW22_01025"
     CDS             40917..42575
                     /locus_tag="PLW22_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU73312.1"
                     /translation="MVKYNYVKEFVEKPIRKHWELNSLSDFKGETFTYADVARKIHYL
                     HKLYQIWGVKTGDKVALVGRNNAEWGIVYLSVISYGAVIVPILPDFSPTEIHNIVNHS
                     DSVLLFAGDMVWGGLNQAEMPNLKAIFSISGFNLLVSNDEESRRLFEAAKSSITDEMK
                     SLTPDKVNYPEVPNSELGVISYTSGTTGFSKGVMLPLNSLAANTDYARNNMHLVKGDQ
                     IVSFLPLAHAFGCAFEFLFPFAMGCHITFLTKIPSPPIILEAFAKVRPRLVLTVPLVI
                     EKIFKKQVQPKIEKPAMKVLLNIPLLNKVIFKKINEKLSNAFGGNFHEIVIGGAALNP
                     DVERFLRRIKFKYTVGYGMTECGPLISYSGWRTNPMGSCGKVINYLEMKIDSSDPYSE
                     VGEILVRGENVMMGYYKNPEATQKALDNGWLHTGDLGVYDSKGFIYIKGRSKSMILGP
                     SGQNIYPEEIEARLNNIPFVQESLVIEQKGKLIGLIYPDYESVQQQGIDPDKLEPVLE
                     HHRLEVNKHLPQYSQIAKLVVHNQEFEKTPKKSIKRFKYQYSDN"
     gene            complement(42572..42991)
                     /locus_tag="PLW22_01030"
     CDS             complement(42572..42991)
                     /locus_tag="PLW22_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73313.1"
                     /translation="MNQQAKEHILHFWTRNLVEKPGAFSFNLFLFLSFGILYSFRILQ
                     SPFILLVFGIITPVILTICLYHMSGVSLQHLLPKVFHKKTSRVFLALLDCSIITLLGI
                     LIYRGILNFFFFRFLQTVMLPILYLIMLRVLLLSEHN"
     gene            complement(42998..43951)
                     /locus_tag="PLW22_01035"
     CDS             complement(42998..43951)
                     /locus_tag="PLW22_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOU73314.1"
                     /translation="MNLLFIILGVVILVLGATWLVEGASSIAKRLGISALTIGLTVVA
                     FGTSAPELSVNLISAIEGNSDIALGNILGSNIFNTFMILGLAALVKPIGVQHSTVKVE
                     IPFFLLSALVLWVLSNEVFLDSAELNVISRSAGLVLLLLFCVFMYYNFLVGKEDPASA
                     SPEIETRKLWMSVLMVIWGLAFLVGGGKLIVLGAVNMATKLGVSQSIIGLTIVAAGTS
                     LPELATSVVAAYRNKPDIAIGNVVGSNIFNILLILGLTAGINPIKLYSGANLDLALNI
                     AAGILMLVFAVSGPGRKFDRREGVILVMVYMAYLAYQIIKA"
     gene            44083..45795
                     /locus_tag="PLW22_01040"
     CDS             44083..45795
                     /locus_tag="PLW22_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU73315.1"
                     /translation="MKRILITVGASVLVLLMFPRCAKVVSPTGGPKDTIPPVLVNSNP
                     KLNATGFSGQKIVLEFDEFIQLKELQQKLLVSPPLKSKPQPKQKGKKLELELTDTLKP
                     NTTYTFYFSDAVRDNNEGNPIKNFIFSFSTGQTIDTLTLSGRVLNAITNEPVENALVM
                     LYDSFTDTLPYTTLPNHIAKTDKDGKYTLVNLKPIKYKLVVVDDKNGNYLYNQGSESI
                     GFISYEVNPSDKNPKSISIRLFREELPNQIVTGYDRPERNILSLGFSRKPVGGFSLKP
                     VDRLKLKNWYVVEPDNNGDTLKLWIVNDEIANRDTLMAILEYQKTDSLYRLKPQTDTL
                     RFLYYKEEVQQSKSKGKKDEAVQVKKGFTVSTSLPSDRMVIPNVPFELTLPIPIRKAD
                     NTKISIFNTTDSIADEQIKLKVDSLNPRIFRFEKEWKPNTSYKLTILPGTFEDYTGQT
                     NDTLMLEMIGADPEKYGTLRINLTGVSKSAVVELLSEKGIPVDARTIQGNGIVEFSFV
                     KPGKYRLRFIDDSNNNGKWDSGSYMKRIQPERVIYFTDEKTKGVINIRANWDSELQFS
                     LKQE"
     gene            45799..46110
                     /locus_tag="PLW22_01045"
     CDS             45799..46110
                     /locus_tag="PLW22_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOU73316.1"
                     /translation="MSVLMNFAMFPTDKGASVSPYVAKIIELIGGLGYEFRLTAMGTI
                     VETPTLQQALDVVDKAYKLLEPDCERVYVSINLDIRKSDLGRIDGKVKSVTEKLKKSN
                     Q"
     gene            46117..46773
                     /locus_tag="PLW22_01050"
     CDS             46117..46773
                     /locus_tag="PLW22_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU73317.1"
                     /translation="MIKKSLTVLLTIFALSAYSQTYVGVKGGYTMSDIAFSPVQNTRM
                     LFADGFDYGLVFKYFNLKYFGLQAELYMVNRGYRKPIDLAELGDTLYKRVNTYIELPI
                     FMQVRFNLKLFMIHFNAGPYIAYLVQAREGDNSSGEYQMSNISINILRDNRIDFGVLG
                     GIGLSRDFKWGTFFAEGRVGYGYGDMQKHTYTGMPKQSKAVFQSVNVGYLYHFGNNVK
                     "
     gene            46832..47497
                     /locus_tag="PLW22_01055"
     CDS             46832..47497
                     /locus_tag="PLW22_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73318.1"
                     /translation="MKSWNEIPYDIQRVISNTAVAPGAYILKFKKNYPFIAGQVLALG
                     VSKEIEPRYYSIASPENDELISILYTERVEGKLTPMLSLLKPGENIMVSKPFGTFTHV
                     KPSPVFIAAGTGIAPFMSMLLTGKASGVTLIHGAPYPEYFYYSDTLISRLGENYIRCC
                     SRCQPGCLFVGRVTDFVKQWNDLDVNRPYYLCGSAEMVVETRDILISRGVPVSNINSE
                     IYF"
     gene            47501..48523
                     /gene="rlmN"
                     /locus_tag="PLW22_01060"
     CDS             47501..48523
                     /gene="rlmN"
                     /locus_tag="PLW22_01060"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOU73319.1"
                     /translation="MVPLYGKTLDELKSIVTAEGLPSFTASQIADWLYHHHADSIDEM
                     TNLSKKAREMLSAKYTVGLSKPERVSKSADGTKKYLFPVANNRFVEAAYIPDDDRATL
                     CVSSQSGCKMGCLFCMTAKQGFQGNLSAGEILNQLRSIPEFKNVTNIVYMGMGEPLDN
                     LENVLKSLEILTSKWGMGWSTKRITVSTIGLIPAMKQFLEKSNCHLAISLHTPFDDER
                     RMLMPIQNVYPIADVVDELKKYRFSDHHRVSFEYIMFDGLNDTPRHVKELVKLLNGIN
                     CRINLIRFHPIPDSPLRTSPDERIIQFRDSLTAKGIFTTIRASRGQDIQAACGLLSTK
                     ALQEKR"
BASE COUNT        15013 a         9199 c         9543 g        15019 t
ORIGIN      
        1 ttgttattgt gttatttatt agatgattaa agtcagagtg aattaaagga gcggtgaata
       61 ttccaactaa gcctgaaaaa cttcggggtt gaaggcctag ccgatataac gatgtgcctt
      121 caattatctc taccagcttg actagccata tcaaaaggat aaacagaaaa gcaatttgga
      181 ggcttatctt gaatcgagtt cgttcaattt ttaggatagc atcactcatt tctacagtta
      241 tcaataaatt ctcttttgac aagcttagga tccgacaggg tattgtaaaa catatcgatg
      301 tactctaaaa tatcattctt attccgaact gttaaaagcg aatcggcggt taagagattc
      361 attatttcag gtttcttttt tataaaaaaa tcaatggtaa aattcaattc tagcttttgg
      421 cgacaattac ctcgatagta acgttctcta acattggtaa ttggaataat ctcgggaggt
      481 tttgtatagg ggggggtcac aaatccgcaa aaatcaaaat cgtaaggaac tggtataact
      541 gaggttaaag aatcgtggta taataatttt atgttgtgta gcttaggaac tgaccaatcg
      601 gtattgccaa taaggtactg gaacattgtc attacgtcaa gaaccggtct ataggtggca
      661 ttttgaataa tcccttgtgt ctctatctcc ttggtttttg ttcgctttgc cagccatttt
      721 acaggttcaa ttaaaattcc ataggtgatg aaattcttgt acttgccaaa cctatcaaga
      781 tatattaacc taactaatcg taccttaaag ctatagtccg aaagtatgtt atagatacgg
      841 tatgcgtagt actcctttat tatatgttgc tgggcacgtt tggtgttcat acagggatta
      901 acaagcttta tcctttgcga tgtgttaaat attggatcgt taattttttg cggtttaatg
      961 gcaaggggtg gtattttgca gtttgattta tcgcgccgaa agattcctcg tgtcatggct
     1021 ttggcaggga gcgatactga ggtatcagca tagagatagt aaataataat gtcgtgataa
     1081 cgagcgtttt ggggattaac gtcgtagaat agttctttta aatcgccaat tatttctatt
     1141 tccagaattg attgttcttc aaatagattc ccttgtgcat atgaacttat agttacgatg
     1201 cacaaaataa atatttgtgt tattttccta atcctactca tttaattaag gtgcattgac
     1261 catttattga acaactccaa aactttcgag tttcatcaat gagtaaccct tggggaacaa
     1321 tacccctgcg ggcaaaagga acattgattg gaaataacat tccactataa agcggagcaa
     1381 tactatcaac ttcagtatga cctataacca tttggcttgc cccaaatgtt tttaaaacat
     1441 agctaacttc actcatagtt gctttaggta ttgtgggagt ttgtagcaag taacccctgt
     1501 accaaagtaa accatattca gaaaaaatga ggttgagctc attaagaaaa acatcggggt
     1561 caattaaaaa agaattgtcg gggttataca atttatcaag tagctggttc attcgtacca
     1621 atgggatatt tttttgcgca agctgatacg atattccggc atgaacaaag aggatgctat
     1681 caattactat tccagcctta aagtttcgga ccatagcacc gggccaaacg tttttggaaa
     1741 aaagatctga ataggatatt ttatttccgc gtgttaatat ctgatacttt tcgtggatat
     1801 atcgattgtc gttgttcaat ataatcgtct catggtttcc catcaatgtg taaacccgcc
     1861 cacccgattg ttcagcttgc tcagttaatc gataaaccaa ccaaagaacg tcggtaacgc
     1921 gttcgcccct atcaaccaaa tcgccaataa aaactaaatg cccatcgcca aaaatccagt
     1981 ttagattttt atcaactatt gagttgttaa ccagcaactt tgctaaactg tcatattcac
     2041 catgaacatc gccaactgca aatattttaa cctttgaatt tattaccgtc tcattttcgg
     2101 aatacttact aggtttattg aagttaacgg tgtaattaat tgaatcagta gcataaaaac
     2161 ctttaaagga aattacacca ttggtggccc taaaggtttt tgatttaaca aaaacctttg
     2221 aatttatact gtcattaacc aggtaaaaga ctctggctcg gcgtttacta attctctcaa
     2281 catgtggccc atcgaaacag gatgggtaaa ttcctttgga gtaagccacc atgtaatcac
     2341 ggagaaggtt agccgcctcg gttttgccaa atgatagggc gtactcaaga gagttgtagt
     2401 tctgaaagtt ttggaaaaag ggatttgccc taaatcgcat caatacttta agggtgttta
     2461 cagagccaac ccttgctgca taaagtaatg gagtattacg ttgaatgtta tagacatcaa
     2521 ctattgctcc gtgggtcagc aagtattcag caatagtatc cttatcgtat attgcacact
     2581 gaattaacgg tgacagtgtg tttaacacaa tatttacatt ggcgcctcta tcgactaaat
     2641 tctttataag atttaaattt gagttagaaa ttgcgagggt taaaagtgtg tgcgaattat
     2701 taaaataggt tttattaata ccatttactt taattaaact atctatctta ctaatgttgt
     2761 tttcattaat aaaacttaca accaaggaat cattacttgt gttgtcctga gcaaaaccat
     2821 gaacaaataa aaagggggta aatacaagaa gatgtaatgc taatttaaat ttcatcttat
     2881 ttaaaatttt attccccaaa gcaatacatc gtctatttgc tcatttcgtt tcatccatga
     2941 caaaaaggtc ttttcaaaat gagtgcattt tgaaggacca tccatacctg aggtcaatac
     3001 gagttgttcc ttaaaccttg atcgcatgaa ctttttatgg ttatcgccac caagctgatc
     3061 aaaataccca tcggatgtga gatataacca atcgccaggg gtaagtttaa tggatttaac
     3121 agtaaaattt ttgctgcttg caccagccaa tttggctcca agtgttacaa aatcgggttt
     3181 aagctcttga acatcgccat ttcgttgcag gaataaagga attaatgcgc tggtatactc
     3241 aagtagatag gtttccttat caaacacaca tacagcaata tccatgctat ctttaagaat
     3301 catttcattt ttggacttac taagtcgttt aattagttga tcgtcaataa atgaaagaat
     3361 atcgtcagtt ttgtaaagca tgtgttggtt tactgcctga tttattaaat cgataccaat
     3421 tatactcata aaagcacccg gaacgccatg ccctgtgcaa tcggctaagg caaagaatac
     3481 tttatttccc ttgggaagca tccagtaaaa atcgccgcta acaatatctt taggacggct
     3541 aaaaataaaa gtatcggaaa actgtttttt cacaaaactt ataggaggct gaatggattc
     3601 ctgaattcgc tcagcgtatc taatactatc ggtaattttc tgaatgtaac tttcaataat
     3661 attataggct tcttccaggt ttttgttctt ttgttcaatg aaatccttat ttttttcaat
     3721 ttcttgcttt tgttgctcaa tctcctgatt tttctcttcg agtaacacat tttttagctc
     3781 aatctcctca agctgccttg caagcttttc ggcagttctt cgcttatccc tcagggcaaa
     3841 gtaaataacc agcaacatgg ccccgctaag caaaagccca atcaatagga cataggtttg
     3901 gattttagtt ctagtaattt cccgttgctt taactcttgc tgctgaagta atagcttaat
     3961 ttcattctct ttcttttctg tctcgtatag tgtttgtatg gtagatatgt gcctgctatt
     4021 cttttcgttt aaaacgctat ccttgtagct tgatgatagt ttaaagtact caagcgcttt
     4081 gctatattca ttttgctttt ggtagtaatc cgataaaacc tcatacgctt gctgaaaatg
     4141 aattaaactt tttatttcct tggcaatact gaggcatgca aaaacgtttt cgtaggcact
     4201 cctatcattg tttaatccta actgggctcg ggctaagtta atgtagctat cggctataaa
     4261 ctttttatcg ttgagctttt tatctattac aagcgcctta gtaaaataat cttgagcctt
     4321 taatggattt cctaagtaaa gttcgatttt acctaaagca ttaaagttga tagctatacc
     4381 tttttggctg ttgatttggt aattgatatc aagcgatttt gagtagtaat cctttgcctt
     4441 aataaaatca cctttaaatt cgtaaacctc gccaatatta ttaaagttca tagcctggcc
     4501 aagcagatta tttgtttgct gggagaggta gagggctctc tgaaagtatt ccaaggcttc
     4561 atcgtagcgt ccgtttagcg aaaaaatgtt gccaaggcta ttgcaggcca ctgaaatatt
     4621 gaatgtgtcc tttatctctt cggccaactt taaggctttt agatgatact cagaggcata
     4681 agcgtggtta tcgagccggc gataaaccac tccaaggtta ttgagcgatt ttgcctgttg
     4741 atgtttatcg tcgatttctc ttgcaagatc taacgcatcg ttatgaaact ttaatgcatt
     4801 tgcgtagttt gaaatgtttc ggtatagcac accaagcgag gtatatacct ccattcggag
     4861 gtaattgctt tttattgatt ccgccaagcg tttagcctca agtaggtaat tttctgctag
     4921 aacagggttg ttctcattta cttcataaac cctggctatt ttttgtagaa tgagtacgcg
     4981 caagctgtcg ttcgattctt ggtatagttg aagcagcagt gagtcggcat tctgcggctg
     5041 tgcaaccaaa tgcaaacaca gaatggcata aactggcaat aatatctttt taaacatcat
     5101 ggcaaaaaaa ttccttcatt tggaaagata gcataatttc gcaacaaata aaattacacc
     5161 caactagtaa caaatatatg gcaatattgg taaaaatata tcaccgaatt tttagaatca
     5221 ttattttgct cctgctactg atgcttattc tggtgctcac aggtgcagga attttgtttt
     5281 tcaagcaaga atacattata aacaccctta gggaaaatct taacaagtat cccgacctat
     5341 ccttaaccta tagttccgtt tcggttaata cccttaaaac atttccaagg ttttcatact
     5401 catttaccga ttttgtatta gtaattaacc taaactctaa atccgacact gttttaaaaa
     5461 cagatgattt tctgattagc atctcccctt ttaaattact aggtggtaaa attgatatta
     5521 aaaatataag agtaagcaat gcaagaataa aatggcaaac caattaccct gaagaacttt
     5581 tcggtagcag tgatagtagt tctgaaagtt ctgtttccgt cctgatttcg gggattagtc
     5641 ttaataattt cttttttgaa ttgttggata gcgttagtaa aacagtgcta tactgctcag
     5701 ggaaaaactt aaacatcaag ttcaaaacgg agcatacgaa gttgctatta tccattcaaa
     5761 gcgagctgag taaaactacc gtaggtgaat attttaaaat taccaatcag catcagctaa
     5821 attttgatat tgaaaatagt gataataagt tgtatatcta taatttagaa tcatcaatta
     5881 aatcattacg tataaacggt tcgggaattt atgactttaa tagccaatta gccgcattca
     5941 ggtttaaatc aaaccggttt caagcacaag atctatcgta cttgccttat cttaagagtt
     6001 ttgaccaaat aaagggtata attacagcag atgcatacct taggctatca tcgggttttg
     6061 aaaaagttga tacctttcag gttaactata atagcaataa attgaaatgg ataactaaaa
     6121 acgaagagac aaccataacc aatttggagg gttatacttt actaactaac aatttttcaa
     6181 agcacttcag ctatattcct aaagcatcta ttgaaagtaa taactcaaaa attaacttaa
     6241 acgcaaaaat aaaagggttc aataacttgg taattttagc taagggatat gtaagccaca
     6301 atttaagaat acaaaaacct gaaattgata ttgaaacaac aggtcaattt aaagcattaa
     6361 tatcgtatgc cccaaaaacg gaaagtatta ctcccctact tcttaaatcc gatctagttt
     6421 tcgaaaacaa gaaaaacatt tcatttaatc agccttcggt taaaggaact atagcgatta
     6481 ataacgattt aaacattgca ggctatttta aatccgattc aaccgatata aactttaata
     6541 ttaatcaagc caatttcctg gaatcctata cccaaaatag gtattcacca aacatatatc
     6601 tttacggtaa ccatatagat tacaacgata tagttcatct aattactgat agaactagca
     6661 atacatctac caaaggggtt gacctaccaa aaatgtcatt tatgctcaat tttaaaagag
     6721 ctacatacaa tcgattaaaa cttaatagcc taaaagccaa cggaataatt aatggcgata
     6781 caatttcagc agattacttt actgccgatt gctttgatgg caacgtttct ggtagattta
     6841 aatcacatag caattcagtt cacacaaacc tttgggttag caatgtttca attgaaaatg
     6901 ttttcaaaaa ctttgataat tggggtcaaa gctacgttac agccgataat ctttcaggac
     6961 ggtttaaggg cattatgaat atacaattca aaagaaatga gaaaggcgat gttgaaatgg
     7021 gctctcttaa gctattctcc gacgttcaag tggttcaagg aaaactcaaa gggatggaca
     7081 gaattaagga attgtccaaa tggctaaacc tggaccaggt taaggttatt gcttttgata
     7141 ccttgaaaaa taagattact attgaaaatc gtaagatttt tattcctaat atggatataa
     7201 aatccaatgt cttgctaatg aatgtttctg gggttcacga ttttgacaac agatatgagt
     7261 atattgctag gattaacata agcaatttgt taaaacgaaa gtttgtgaaa tccgatcaga
     7321 ttgattttca ttccactacc gatggatcta taaatttata cttgaaacta tttggccaaa
     7381 acgatagcta tgaggttgaa tggataaaca agaaaggttt tgaatccgga atacacaata
     7441 ccgttcaaac cgatagcgtt tctatacaaa atagtcctca aataaaacct aaggaaaaaa
     7501 ctgatggtaa tgtaggtgtg ggttacatgc tggagtggga tgagcaaatt gacaccctaa
     7561 agaacgagta aaatgaaaat tcttagtcca cgaaataaat ttctgttaac actaataatc
     7621 gcagcggtta ccgtttcgtt tattacaatc tactttacaa atcgttttgt atcggaactt
     7681 aagcaggagg agcgtcaaaa aattgaccta tgggcaaatg ccgttaaata catctcaaac
     7741 gaaatgggtt cgtgtgagga tttttcactg atttttgaga tagttcaaaa caaccggact
     7801 gtaccagtaa tactcaccga tgaaactggg aaagtgattg gtttccgcaa tattgaatta
     7861 cctgacatcc aggatactgt ttatgtttcg aatcttctaa acaaaatgag ggctgaacac
     7921 gatccaataa caattgaact gcccgatgga tcgaaaaact atgtatacta ctttgatagt
     7981 attaccttaa aaaaacttaa ttactatcct tatattcaac ttattataat tgcattcttt
     8041 ataggaatag cgttttactc ctatggtcag gcacgtaaat cggaacagaa tttaatctgg
     8101 ctcgggatgg caaaggaaac agcacatcaa cttggaactc ctacttcatc gctaatggct
     8161 tgctcatcgc tactttctga gaaatacccc aacgacgaag tggtgactga acttgaaaaa
     8221 gatgtaaaaa gaattgaaac cataactagc cgattctcaa agattggctc atcaccaagt
     8281 ctcgatagta ttgatataac taatttgatt accgattcca taaactacca gagcaaaaga
     8341 tgcccaaaga caattgcttt caacttcagc cccggcattc caatatatgc taaagcaaac
     8401 catatacttc taggttgggt tttggaaaat attcttaaaa atgctgtaga tgctatgcaa
     8461 gggaaaggag ttattagcgt agaggcaact gttgaaaaaa acaacgttat tattgacata
     8521 caggaccaag gtaaggggat tgaaaagggg aagcaaaaag aaatattcca ggctggctac
     8581 accacaaagg aacgcggttg gggacttggg ctaagtttag caaaacgtat tgttgaacag
     8641 taccactcag gaaaaatctt tgtgtttcgt tcggagcttg gggttggttc aacctttagg
     8701 attataatca aaaaagccta gggaacagga tcataaccat gaccacccca ggggtggcat
     8761 cgtgaaattc tcctaaccga caaccataag cctttaaaaa aaccatgttt ttttagcgct
     8821 tcaacactgt aagcggaaca ggtaggggta aacctgcagc tgttaggtag gtatggcgat
     8881 aaaaatattt ggtaaatctt tattaataga ataggaatgt aggccaaaag cctaaccgca
     8941 tatttccata atattttaag ggttttaacc aaattcattt tacttaagat cggttaacag
     9001 tttaacaact cccgattcca attgtttaaa agaaacaacc tctgctgaaa catagataaa
     9061 agccaggtaa accgtttttc ctctttccct gaattggtca agtaagtttt gattaatgcg
     9121 aaaagcctct ctaagccttc gttttgcaag atttctcata tatgcttttt ttaaatacct
     9181 cttaggaatt ataaatatta ctttgtagtg ggggttttcc tcaatgccac cgggtatcct
     9241 ttcatctact tggtatatta acttgattgg ataatggaaa cgtgataact ccgattttaa
     9301 cagcgagtta acctcgtttt ccaaatggat tatatacttt ttcctaagtt tttgaggcat
     9361 acaaaaaagg ggggtagcat tcccccctct ataattttac tttttctttg attgctcttt
     9421 caaaaagtga tctaaggctt gggtcatgct tttaaacgca tcggttggag ctttaacatc
     9481 gagtcttaaa cccgcatctt tcactgcttt tgcagttgta tcgccaaagg cgccaattgc
     9541 tatatcgttc tgctgaaaat cggggaaatt atcgaatagc gatttaattc cattagggct
     9601 atagaacaca atcatatcgt aagggaatgg cttaaggtct gacagatcgc tgctaacagt
     9661 tttatagaat atccctttgg tataatttat cttggccttt gttaatgcct ttggaatttc
     9721 tggtttgtgt ggatcggaaa gtggcaataa aaatttttcg tccttatgct taaggattat
     9781 atccagtaga tctttgaaag tgttatcgcc aaaaaagatt ttacgcttcc tgtaaactat
     9841 gtatttctgc aggtaatgag caattgattc ggttatacag aaatacttca tggtttcagg
     9901 aactgttatg cgaagttctt cacatatcct gaaaaaatga tcaatgccag ttttactggt
     9961 aaaaattaca gcagaatgat ctaggatatt gattttctgc tgacggaact ccttggaact
    10021 taccccctca acatgtataa aagggcggaa atcaagatga accccatatt tctccgacaa
    10081 ttcattatac ggcgatttct cgctctgggg gtcgggttgc gaaataacaa tctttttgat
    10141 tttcaaggcc ctatgttttt aaaaccaaca aaaacttaac tcactctcag caaatacgtt
    10201 acccctaata ttataggcaa ttcaagggca caaaggtaca aaatgtaata caataatgaa
    10261 accctatgct ttataaagat ttttacaaag taaaaatatc gatatagtaa agcgattata
    10321 atcaacgcaa tagatatata gataaatact attcgtaagg gcggcatggt atagtttgct
    10381 gcaagcgatg taggtataag aaagagcatc aaaattctca aattcaaagt attgtgaaaa
    10441 tagagatagt taacctggct tgcattatta gtagccagca ataaagtttt atgtagtagc
    10501 caaatccaaa ttctgtaaag tataagtgca agggcgggta aagctaaaaa gagtaaggat
    10561 ccctgcattg atcctaaaaa ttgctcgagt ataatattaa ctacaaatgt tattgatatt
    10621 attgatagga aatccagtaa acgagaaagc ttaataaagg gcacgctata atcgttaagg
    10681 gttttttcag caataaaatc gtagaatagt gattgaataa aaactctaag atattttgcg
    10741 taacctgcta ttattgatga aattatcagc attgtaataa ccatgctaat aaaataagca
    10801 ttcggaatta aatgattatg gttaaggggt ttaggttcaa tggtgatgtt tgatttaaat
    10861 gaacttgact taaaaacatt tgaacaagaa tcggaagtta aaggaaaatt atcgtggata
    10921 gtataaagct ttccggaatc gtacttctgg gttaaatcca aaaagttgtt gtataaatta
    10981 ttagcattct ggctcgattg aaaggctgac ataaatacag aatatttttg caaaactacc
    11041 tatatttttt taaagaacga cacgatattg ctggctgttt ttgctccaac ctcttccgaa
    11101 agctcttcaa gggaggccat ttttatattt tctaatgatt taaagcgggt gtaaagctta
    11161 ataacgcttt tctttcctac accaggtatt gattccagta tcgatgcttc gttctttttt
    11221 tctcttttct ttctgtgaaa tgttattcca aacctatgcg cttcatcgcg aatattctga
    11281 ataagcttaa gggacattga acgtttatcg aggtacaagg gagttgtgtc gccgggtcgg
    11341 taaatttctt caagtcgctt tgctatacca attatgggaa tatgctcgat atgcaaatct
    11401 ttaagggttt cataggcaga acttagctgt cctttaccac catcaatgac aattaaattt
    11461 ggcaaattct ttttctcttc aatcaatctt ttatacctac ggtaaacaac ctcgcgcatg
    11521 gaagcaaaat cgtcagggcc ggtaactgtt tttacattaa aatgcctata ctcactttta
    11581 gcgggttttc cattcttaaa cacaacgcag gccgaaaccg gattagtacc ttgtaggttc
    11641 gaattatcaa aacattcaat aaccattgga agttccggta gctgtaaatc cttctttaac
    11701 tgctcaaggt attttagggc acgggtttga ggatttgctc gttcctcact tttaattaat
    11761 tcctttatgt aagcatcagc atttcgtagg ctaagcttta aaagcttaag cttttcaccc
    11821 tgttttggaa tgtgatactt tatattggac agctggtatg cagggttaat atttacaaga
    11881 aaagtagtag attttaggtt aagcctctcc ttaatctcta gaattgcctg tgaaaaaata
    11941 tcatcaatag gctcatcaac aatatttttg atttgtaaat tatacgattg aactaccacg
    12001 ccactgttta cctttaagta gttaacaaca gcaaaacctt cgctctcttg aattgtaaaa
    12061 atatcagcag tgtttattcc ctttgaaatt acagaacttt tggcctggaa attttgtaac
    12121 agtctaagcc tttccttaag caatgcggcc tgttcaaatt ttagctcctg agcggctttg
    12181 gtcatttcgg ctgttgttag gcggagcacc tcgttaaggt caccttcaag aatttttttt
    12241 atagcattaa tgttgcttaa ataatcgttt tccttttgat ttccgataca aggcgctaaa
    12301 caatttccaa tctgatactc taagcaagct ttaaattttc cagaggcaat attactttga
    12361 gttaagttta gctcacaggt acgaatggga aaaagctgcc gaattaaatc aagtaatgct
    12421 ttaagtaaac gacccgatgt atatggtcca taatatattg atccatcgtt ctcgtatcga
    12481 cgcgtggttg ttacacgggg aaaaggctcg tttttaatta caatccatgg gtaggtttta
    12541 tcgtcgcgca aaagaatgtt atacttgggc tgatgttttt ttataagtat attctcgagt
    12601 atgagcgcat cggattctga gtttactaca atatacttta aatcggccac ccttgaaacc
    12661 aacgccctta cttttgctga gtcatactgg gaacggttaa aataagacga tactcgcttt
    12721 cgaagattct tggccttgcc aatataaatt attttgcctg ttgagtcgaa aaattggtaa
    12781 accccaggca aatcgggtaa agatgataaa taggatatgc tcgaaaggtt ttccatttac
    12841 atcattccgc aaaaatggag gttaagctaa acctatcaac atcaaacagt ccagctggag
    12901 ttaactttaa atgtggtatt accgatagcg acataaatgc cattgtcata aatggcgcat
    12961 ggagcttaca tccatcggcc ttaagttttt tgttaatcag tgagtactcc ttagcagcat
    13021 cgctcacatc aacatttgtc attaaaccaa aaaacggcaa ggccaaaccc atagtctcat
    13081 ttccatcata gtagcacatt ccacccctat ttttaattat atagtctata gctcgcacta
    13141 tgctgtcgtc gtttgcgcct atggcgacaa tatgatgact atcgtgggca atacttgctg
    13201 caatggcacc tttcctcatt ccaaaaccat tgataagccc aacccctata ttgtttgggt
    13261 tgtatcgact tagcacaaca atttttagaa tatctttatc aaaatcgtag ccagaaccgg
    13321 gtacagatgt ttttaaggtt tcggtgatca actcgccttc aatcacctta ataaccttta
    13381 ccggtttgcc agaaggaaaa tctagcttga ttttttgatg atcaaagtga tttggaaagc
    13441 taaacaacgg gggtttaacg gcataatctt cctcaaaatt ggttacatta acacctttaa
    13501 tataggtaga taatacatta aagtcattta ggttgtcaac aactataaaa tcggccggtt
    13561 ggcccacctg caacaatcca acatttaacc tatagtggtt aacggggtta accgaagcaa
    13621 cctgcagcac atcaaaaata tcatatccta gcgcaatagc tttgcgcaca tgcttattta
    13681 tatgcccgcc cattaactca tcggggtgac aatcgtcagt gcagaacata gccatacttg
    13741 ggttttgccc aattattgga tgtagggcat caaaattttt agcggcgcta ccctctcgaa
    13801 tcagaacctt catcccaagt tcaatttttt tcttagcctc ttcaagggta aaacattcgt
    13861 gatcggtagt aataccggct gaaacatatt tggtaagttt ttcaccgctc aatcccggag
    13921 catgaccgtc aatgggttta ttccttgctt tggcttcgct tagtattgaa tgaacttcag
    13981 tattaccatt aataactccg ggaaaattca tcatttcggc aaggtgggta acctcaggta
    14041 agtctagcag ttttgcaacc tctctagaag taaagggttc aaaacactca tccacgggcg
    14101 atgcaggcac acacgatggc gctccaaaaa agaattttag tttggattgt cttgcattct
    14161 ctatcataaa gcgtactcct tcaatccctg ctacatttgc cacttcgtga ggatcggcaa
    14221 ccactgccac agttccattt ttaattgcag cctgtgcaaa cctagtaggc acaagcaatg
    14281 aactttcaat atgcacatgc gaatcgacaa atccaggaat tatataaaca ttttcagcgt
    14341 ccaaggattc atcaatttgg gtaattacac catcctgaaa gtatatttta gcgtttacag
    14401 ttctacgacc gatagggtca accaattttc ccgagattga attcgaattc ttcataaaca
    14461 tccaattaat gttttactta aactttttgt taaattgttc cacgtggaac actttaatga
    14521 aatatcttaa tacactaata attatatact tatggttttt gaaatgttta tgttaaaaca
    14581 aaaaattgtt ccacgtggaa catcaccttc caaagtatac caataataca ttaatatcgc
    14641 taggagaaat tccgggtatt cgttttgcct gagcgatagt tttgggtttt atgcttgtta
    14701 actttatcct ggcttcggtg cttaatgagt taagctgatt aaaatcaaaa tcgtcgggta
    14761 ttttaatatg ttccaatcga tttatttttt ctgcaattat tttttctttt tcaatgtagc
    14821 cactatactt aatttttatt tctgcgccgt ttatggcttc ataatcgtat gagtcgatat
    14881 ttattttgag gtgctcaagt gttggtttaa gcgatatttc gggacgtaaa acaatatcgt
    14941 agagctttct tttttgtgta atttttgctg aattatttga ggtaaggtat tcgtttatat
    15001 cgctaggcga tacagaatat tctttaacga gttgattcag ctcctcaatg cttttaatct
    15061 tgttttcgag ccgttttaca tcgttttcgt caaccatatt atactttgct gcaattggcg
    15121 ttaacctttc atcggcattg tcttggcgaa ttagtatgcg gtattcggct cggctggtaa
    15181 acattctgta gggttcatca acaccttttg ttaccaaatc atcaatcaac actccgatgt
    15241 aagcttcgtc tcggctgagc tgaaagtttt cttggatatc atttgccctt aagtgtgcat
    15301 ttgctcctgc aattaaaccc tgacctgctg cttcctcgta tcctgttgta ccattaattt
    15361 gtccagcgaa aaacaagttc tgaactattt tagtttctaa agtatgtttt agctgtgttg
    15421 gatcaaaata atcgtattca attgcatagc caggtcgaag aatatgaaca ttctcaaaac
    15481 cgggaattga ttttaaagct ttgtactgaa tatctactgg cagcgatgac gaaaaaccat
    15541 ttaagtacat ctcataggta aaaacacctt cgggctccaa aaatagttgg tgtgatgttt
    15601 tttcggcaaa ggtgtgcact ttgtcctcaa tgcttggaca gtaacgaggt cctatgccct
    15661 ttattttacc agtaaacagg ggcgattgat caaacccctc tcgtaaaata ttatgtacaa
    15721 cctgatttgt gttaacgatg tagcaatgct tacgtggtag ttttgaaaaa taatcggggt
    15781 tgaatgaaaa tttagataat tcttgcgatt cttgtatagt cagtttacta taattaacgg
    15841 ttctaccatc aattcttaca ggtgtacctg ttttcattct cccggttgtg aaaccaagtt
    15901 ttactagacg ctcagttaag ccttgtgagt ttagttcacc tatacgtcca ccggtaaaat
    15961 tgacgtttcc aacgtgtatc ttaccgttta aaaaggttcc atttgttaaa acaacagtct
    16021 tacacttaac tgtttgattt gtcttagtaa ttataccctc tactcggttt tcgtttacaa
    16081 ttaaatcaat tacttcatcc tgccataaat acagattagg tatcgattca agatattttc
    16141 gccattcaat tgaaaagagc attctgtcag cttgagcgcg tggactccac atggctggtc
    16201 ctttgctctg attaagcatt ctgaaatgta gggttgtaag gtcagtcact tttgctgtat
    16261 atccaccaag tgcatcaatt tctctaacaa tctgaccttt tgcaattcca ccaatagcag
    16321 gattacagct catttgggct aacttagtaa gatccattga aactaaaagt gtccgggagc
    16381 ccatttttgc agcaatagca gctgcttcac atcctgcatg cccacctcca acaacaacaa
    16441 catcaaaggc taattccatt ttgagtagaa aatttttaaa gcccagttct ccttttctct
    16501 aatttccttc ttttgctcct ctgtagaatc gtcaaatcct agcaaatgta atattccgtg
    16561 aacaattacc ctgtgtaatt cattttcgaa ggtaacattg tagtcctttg cattagtctt
    16621 tacggtatcg actgaaataa caatatcacc agaaattgaa ttttctgtac agtagttgaa
    16681 agtaattata tcagtataat agttatgagc taaatacttt ttattgattt caagtatttc
    16741 agtatcgtca gtaaaataga agttaatatc accaactgat ttcttaaact ctttttcaat
    16801 aatataaatt atccactttt ttattttgtt tttattggat atcctatttg agatggatgg
    16861 tgttttatat gaaaaaaaaa tagccattaa atattaaaag tcatttttac tgttgttccg
    16921 ttgttctcaa aagcaatttc atcagacaac tttttcataa gaaacacacc tcggcccgaa
    16981 atgttttcaa tgttttccgg aatagttgga tccggaattg tatttggatc gaaacctttt
    17041 ccctggtcag aaattgttat gcatacttta gtatcgcata tttttatatt taccttaaca
    17101 acttttttct cgtcaagttt attgccatga acaatagcat tatttacacc ctcgatagca
    17161 gcaattaata tttttccata cacatcgggt gttaaagtaa gtttttctgt tatttcatca
    17221 atagtttttt cgattaaact aatttgctct agcttgcttg gaattgtaat tgttcgttcc
    17281 atggctttta ggttgttatt tttttatact ttatttttta gtaaaggtta cgcaaaatta
    17341 tgaaaaaaat gattttacta aaatttcaac cagtctaacg gattttcagg ttccttctca
    17401 ttccatatct caaagtgtaa aacaagtgta ttatcggttt gtttcattac acttcccaaa
    17461 ttatcgccag catttacaag ctgtcctact tttacactta cttcactaag attagagtaa
    17521 acgcttaaat attcaccatg cctaataata atagcccttc ccccatatgg aacattaaat
    17581 atttttttaa cctctcctct gtgaattgct tgaaccgttg aagaattgtt aagtattaaa
    17641 tcgattccgt tattctttac ttttacattt ttaagtattg ggtgattgct ctctccaaag
    17701 tagtttaaaa cgttgctatt taatgcaggt ggtggtaatt ttcctttgtt ctccttgaaa
    17761 atattactta attcattata tctcttagca ttttctttat tcatagattt tagagctctt
    17821 gcattctctt caattattct tttaatttca ttatcaatta atgctaatgc ttttttctta
    17881 ttggaaattt ctttagttaa ctcaattttc tttttctcta aatccttttt agttgcttct
    17941 aattcatttt tttccttatt tagagtagtt atcgtttttt ggtattcatt ctccttaagt
    18001 tttagttctt ttaaaaaaag cacatagttt gacctcttct gttctaattc aatcttattt
    18061 tcattaagta atgatatttg ttgattgaaa taattaacaa gatttttata cattttaagg
    18121 cgggtataag cttgatttat actacttgat gaaagtagaa gtattaataa atcatttttc
    18181 ctctttaact ttttaaagta tgatttataa attctaatta attcatttct tcgagtatca
    18241 ataagagtag ataaactatc aacttgtgag gtagtctcag ttaggttttt cgaaatttct
    18301 gttatttgat tttttatatt tataagtaac ctgttttgta actcaataga ttttaatttt
    18361 aaggttaact cgttaattgt agcttttttt gatttattaa ttatatcgat ctgtttattg
    18421 ttatcgtcta tttgttttag tatttccttt ttttgattat tcaaaatatc tattttattc
    18481 tgtgaaaagg taaaattcac agaaaaaaga aaaactaaaa ctaaatttat tataactttc
    18541 attatttatg accagttgat ccaaaaccgc ctgcacccct gtcggtttca gaaattttgt
    18601 ttacgttttc aaaagatact ttttcatacc ttgtaaaaac catctgacaa atcctttcgc
    18661 ctggttcaat ggtaacaata tcattgctta aatttacaac aataactcca atttcaccac
    18721 ggtaatcgga atcgatagtg ccaggagtgt ttagtataga taaacctttt tttaaagcca
    18781 taccgctcct agggcggatc tgggcttcaa taccaacagg taattcaata aaaagtcctg
    18841 taggtattaa ttttctttct aatggcttta aatttattgg ctcctcgata aatgctttta
    18901 aatcgacacc gctggaatgc tcagtagcat atgtagggtt atcgaacgtt gatttgttaa
    18961 gaattttaac tatctccata aagtaatttt tatgttttct ttcttaatga atttgtaaat
    19021 aaataaacca attaaaatat tagctgctat tagtgaaaaa tagggtttta agaataccaa
    19081 taataaagtt ccagataaag taatgaataa tataaaaata atataaaatt ttatgttttt
    19141 agtttcgaac gaaacaaatc cagatgttct accatcgcta taactcataa tagtcatagt
    19201 tacatatgtg attaaacggc taagagcagc accataaatg ccaattattg gaataagtat
    19261 tatattagaa attatagtta taattgatcc aataacagtg tactttaaac cataaatagt
    19321 tttatcagca agcttatacc aaaaggaaag gttaaagaat agaccataaa aaatatttgc
    19381 taaaagtaat ataggtacta tataaagcga ttcacgataa tttttgccaa taaaaaactt
    19441 tagtattggt aaattaaagg ttattaggag ggatataaca attgaaaaga ttagaaaata
    19501 cttaaatgac tttccaaaaa gcttattgtg gtcctgactt cctgaagatt taaagaaaaa
    19561 aggctcagca gcaaaacgat acatttgtac gaaaagtact aaaagtacag ctagtttaac
    19621 acaagcacca tatatcccta attggtaaag cgagtcgaca cttttaggta ataagtactt
    19681 gataaaaagt ctatcgaaag cctcgttggt agtaccggat aatccggcta ataaaatagg
    19741 gaaagaatac caaataagtt ctttcatcaa acttttatcg attctaattt ttgggaaacc
    19801 agtaaaatag gtaaccaata caaaaacaac aatgttctgt ataaagttag ataggaatac
    19861 ataaaacaaa aggtcgatat gtgggaataa attttttaag tatgagggtt taagaaccag
    19921 gtaaaaaata atatttaaca atacatttat ggcaacaccg gtaattttaa taaacgaaaa
    19981 tacaaggggt ctattctggt ttctaagttt ggcaaaaggt aaagcggtga tggaatcgag
    20041 cgatacgatt gctgcagtag tgataatgta aatgggtaaa tacccagatc ctaataaact
    20101 tgaaattgaa tctgaaaaaa ggtaagcagc aatgaaaaaa atggatgaaa gtgttagaac
    20161 aaaagtaaat gcattagaat aaacccgctc atcattttta tacttattta caaaacggaa
    20221 atagccagtt tccaaaccaa aggtcaagaa tattagtaaa aaactaatgt aagcgtaaaa
    20281 ctcagtaatt acaccgtact gcctagggtc tgttaaaacg ttagtgtgta taggaacaag
    20341 caagtagttt agtaaacgag ggacaatggt acttaatcca tatataattg actgtgaata
    20401 aagttgtttt acggatgaca tttttccaag tatgaatgtc aaaagtataa tttttggtat
    20461 ttttgcacaa gtaactatga aagataaaag ttaaagcaat gaaaattaga actttagttt
    20521 tactcggtct ggtatcaatg ctatcgtgca ctacgaatta caacgataac aatagatatg
    20581 ttaaaatttc aactgatgta ggagatatag ttattaaact atataatgaa acgcctaagc
    20641 atacagaaaa ttttatcaaa ttgaccgaag agaatttttt cgatggtctt attttccaca
    20701 gagtaataaa agactttgtt gttcaaggcg gtgatcctga aactaagaat gctgagccag
    20761 gaaaactata tggcgaaaag gatgccggtt atttaattga tgcagaaatt gttgatacaa
    20821 tttaccataa gcgtggagta atagcgatgg caagggaagg cgatgatgtg aacccagaaa
    20881 aaaaatcttc atcatcacag ttctaccttg ttgtaggaaa ggtatttact gatgaagaac
    20941 ttaataagct tgaagacaag cttaacaaaa aattaaggga gaaaacagaa aagaagcttt
    21001 acgatagtct tttaggtagc caaagcaact taaacccaaa atttcttaat caagtaacac
    21061 aaaaggtttc tatcttagct gactcaatta tgaatgttaa acacatcaag tttaatgaaa
    21121 atcagcgtaa aatttatact actgtaggag gcataccaca cctagatggg aactatactg
    21181 tttttggaga aattgtggaa ggatacgatg tggttgaaaa aatttcgcag gttcaaaccg
    21241 attccaacga tagaccttta aaagatatta agttcaaagt tcgcattgta aaatagttgg
    21301 taaaatggag gaaagaatta aacagataag ggaaacgttt tcgacattag atcctaaaga
    21361 cctacaaagt gttgaaaaaa taagagttga gtatcttgga aggaatggtt atctgacaaa
    21421 actatttgat gactttaaaa atattccaaa cgaaaagaag aaggaagttg gtaaaatact
    21481 aaaccagctc aaatctgaaa tatccgataa ggttgaaacc tggaaaaaaa cattttccga
    21541 atctacaaaa tcaaaaggaa ctaatttaga tctttcgcta gaggagtatc catttgaatt
    21601 tggaacccga catccaattt cactagttaa gaatgaacta gttgaaattt ttaaatattt
    21661 gggttttact gtttatgagg gtcctgaaat tgaggatgat tggcacgtat tttcggcctt
    21721 aaactttccc gaggaacatc ctgccaggga catgcaggat actttcttta ttgaaaaaaa
    21781 gccagatatc cttctgcgaa cacatacatc atcggttcag gttagaagca tggaaaatga
    21841 gaagttacca atcagggtaa tttgtccagg cagagtattc agaaatgaag caatttcagc
    21901 aagagctcac tgtatttttc atcaaattga aggactttac attgataaag atgtttcatt
    21961 tgctgatttg aagcaaacac ttctttactt tgcaaaagag atgtttggtt cggaaacaaa
    22021 aatcaggctt agaccttcat actttccttt tacagaacct tcggctgaaa tggatgttag
    22081 ctgtaagctt tgtggtggaa aaggttgttc ggtttgtaaa tatactggct ggttagaggt
    22141 tttaggatgt ggcatggttg acccaaatgt actagaactt aacggtattg atagtaaaat
    22201 ttattcaggt ttcgcatttg gtatggggct tgaacgtata gcaatgctta aatatcaggt
    22261 tcgcgatttg cgtctttact ttgagaatga tgttcgtttt ctaaaacagt tcaagaatta
    22321 ctgaaaatgc tgaaagacgg aaattcaaat acttgtataa gttcaagttt tccaattttt
    22381 aaatcgctta atagcgagga acttgaacta ttgaatgcaa acttaaaatg taacacctac
    22441 cgaaaaggtg aaataattta tagagaggga agcaggatca cgggcattta ctgcgttaaa
    22501 aaaggtattt taaaaattta caaaacagga attgattcta agccacaaat tgttgcattt
    22561 gcaataaagg gggatataac aggttacaga tcggttttga gcaacgaaat ggcttgtaca
    22621 acagctgaaa ttattgaaga agcagaaatt tgctttattc catcggaaat tatctttaac
    22681 ttaattaaaa aaaatagcga ttttgctctt tcattaatac agctaacatg tagggaattg
    22741 gatcaggcaa atatatttat aaaagatata gctcaaaaaa ctgttaaaca gcgccttgcc
    22801 gaaatcttac taatgctgga aaatactttc aaactagacg cagagggcta tatagcagta
    22861 aatctaacta gagaagattt atcgagtatt gttggtacag caaccgaatc tgtaattcgt
    22921 attttgggcg aatttaagaa cgagggtttg atagcaatta aagggaaaaa aattaagata
    22981 aacgataaga aaaagatgaa tctgatatcg gattctattt tttagaagag cttaccttgc
    23041 tcaggaaatc ttaatttatt taaatgctta taggctaaat cggttacttc gcggccacgt
    23101 ggtgttcttt ttaaaaatcc ttcctgtatc aggaatggtt cataaacctc ttcaatggta
    23161 ccagcatctt caccaactgc agttgcaatt gtatttaaac caaccggacc acccttaaac
    23221 ttttcaataa ttgttagtaa tattttattg tccatctcat cgagccctct ggaatcgatt
    23281 tttaaagcat ttaaagcttc ctttgctata tcaatatcta tataaccatt accaattacc
    23341 tgagcaaaat cacgtactct gcgtaatagt gcatttgcaa ttcttggtgt tcccctactc
    23401 cgcattgcta tttcaacggc tgcatcatct ctaatatcaa tttttagaat tgatgctgat
    23461 cgttttacta ttcctgctaa aatatcaaca gtatagtact caagaagaaa ttttattcca
    23521 aaacgagccc ttagaggact tgtaagtaat ccactgcggg tagttgctcc gattaaagtg
    23581 aatggattta attctatttg tattgaccgt gcgcttggac ctttatcaat aattatatca
    23641 atcttgtaat cttccattgc tgaatacaga tattcttcaa caactggact taacctatgt
    23701 atttcatcaa tgaaaagaat atcgtgtggt tcaaggtttg taagtaaacc tgctaaatcg
    23761 ccaggttttt cgagtactgg tccggaagta gttttaattg aaacgcctaa ctcattggca
    23821 attatgttag ctaaagtagt ttttcctaat ccgggaggac catgcaataa aacatgatct
    23881 agtgcttcat ttcttaactt agcagctttt acgaatattt ttaagttctt tacaatatca
    23941 ttctgtccgt tgaattgatt aaactcaact ggtcgaatat gcttttcaaa ttctttatct
    24001 attataattt tactgtccat taccttatag caatcattct ttcaataggt aactttgctt
    24061 ttgtaataat ttcaggactt aaatcaacaa ccggatactc atattttaga cttaaatata
    24121 gcttttttaa ggttatcatc ttcatataaa tacaatcgtt acatgcacat gtagaattgt
    24181 caggaggtac aggaataaaa tctttttcgg gtgaaaactt cttcatttgg tgtattacac
    24241 catactcagt tgcaactaga tatgaagttg aaggatcatt aatagtaaaa tcaataagtt
    24301 gtgaagttga accaatataa tcagccatta taagaatagt tttctgacac tccggatgtg
    24361 caattatctt tgcatttgga ttctgtttct taagttcaat tagtctctcc aatgaaaacc
    24421 tctgatgaac atgacaaaaa ccatcccaaa taatcatatt ttcacgcttc aacttatttt
    24481 ttaagtagtt acctagattt ctatcgggtg cgaatattat aggtttttct attggaatag
    24541 attcaattac tttctcggca tttgaagaag tacaacatat atcggaaaga gttttaatat
    24601 cggcattcgt atttacatag gttataacta tgtgttcagg atatttttct ttgaatgact
    24661 tgaaatcatt ataattacag gaatcggcaa ggctgcagcc tgcattcata tcgggtaaaa
    24721 caatttttgc atcaggtaac aaaattttgg tggtttcacc cataaatttg actccagcaa
    24781 atattattag tttcgacttc aatgtagtgg cctttacagc taaagcaaga ctgtcaccta
    24841 caaaatcagc aatatcctga atttcggggc tttggtagta atgcgcaaga atcacagcat
    24901 ttttctcaac ctttaaacgg ttgatttcat ttacaagatt gatacccgaa tcgataggtt
    24961 cattgatata tcccaactta tcaacagatt ctttatttat cattaattct ttttttaaaa
    25021 atttaaaaga taaaaaaagg atgatgatat taagctgtta gctcggttca taattttatt
    25081 gaaaagggtg ttgcaaaatt ggttattaaa tggattaaaa caaactatta acatcataaa
    25141 atttataagt cattgtaata caggatatat ttgtacttat taacagctgt tatcaaggtc
    25201 atatgttaat aaaattaagg gtagagaatc tgttagctga ggttgaaaat gccttaataa
    25261 tttggttaaa caaattcata ttaacctatg gttggttgga aaaaaacatt atatgtaggt
    25321 ttagtgaagc aagtcaaggg ttaagtttaa aaaaatatta acaggtattg aaccaaatac
    25381 tcacatcaag ctaaatatgc atggttggtt ttgtttatat ttgaagtgga atcaaaaagg
    25441 tttgtaatga aatcaaatcg ccttggtatg tttgtattta tggccattgg ccttatttgc
    25501 ggacattctg gttggggtag caacacggat acgctatgcg tagacaagaa aagtaatgat
    25561 aataattact ttgtagtagt acccgttgca ttttatggag aagagacaaa ctggggcggt
    25621 ggattatcgg gtggatacta ctttagatgt agggataata aggtatcgag cgttcagggg
    25681 actgccgttt atacccttaa gaatcaggca agtttatgga tatcgcctag aatatatacc
    25741 actgatagag aacactacta ttcgggtcat ataagggtaa accatttccc aaacaaattt
    25801 tggggagttg gaaggaacac acccgacacc ctggaggaaa agtatgtttc cgatgattac
    25861 tcggttctta tccaacatca aagaatactt tttggtgtaa taatggctgg tgttcaaggt
    25921 caacttaatt actacagaac tggagactac cccggcgacc gcttgctggc aaccggcata
    25981 tctggagtaa acgaaaggtt aacaacgggc ttgggtgtcc ttttaacctg ggaaaacagg
    26041 gagaatcttt actacccatc gtttggtgag ttctataaag cctcgctaat ggtttacagt
    26101 aagatatttg gcagcgagtt gaactttaca aagctaacca tcgatttaag aaactactat
    26161 aatatttacg acgatcatgt atttgccctt caattcctag ccgatatgac ctgggggaaa
    26221 gttccttttc agctaatgcc aatgattggg ggagctgatg ttatgcgagg ttactaccag
    26281 ggccgatacc gcgacaacgc aatggcttgc ctacagggtg aatatagatt tccgatatat
    26341 aaatggctta aagggactat ttttggtggc ttaggcgatg ttgcacctta ctttgacaag
    26401 ttagatataa gcaaaagcaa gttagcttac ggagctggaa taagagttag ggtaaaccct
    26461 ataaaggtaa accttaggtt tgatgcggcc tatagcgata ggaaagaaat ggcctactac
    26521 tttacggtaa ctgaagcatt ttagagaaaa aaacccatcg ggtttggtcc gatgggctag
    26581 tggttacaaa ggtcagtatc aatgcttcat caatgcttca atttcttcaa ctgtaaccgg
    26641 aatgcgtttt ccaagaatcc gggagccatt atcggtaata agaatatcgt cctctattct
    26701 aatgccacca aaaccaatat attcctcaac cttggcatag tttataaatg attcattaat
    26761 cttttccgac ttccattttt ggattaaggc aggaataaag tagataccag gctcaaccgt
    26821 taaaacaaat cctttttgca agcgacggcc caaccttagg taggctatac caaactggtc
    26881 ggcgcgggtg atttcatcgt cgtagccaac gtagttttcg ccaaggtcct ccatatcatg
    26941 aacatcgaga cccatcatgt gcccaaggcc atgaggaaag aagagggcgt gtgcgccatt
    27001 ggcaactgca tcatcgggat tacccttcat taagccgagc ttagtaagcc cttcggcaat
    27061 tacacgcgct gcagccttat gaacatccat ataggggatt ccgggctttg aaatttcgat
    27121 ggccttgtta tttgcagcaa gaacaatgtt gtatatctct ttctgtttta cggtgaattt
    27181 accatcaacc ggtatggttc tggtaaaatc ggaagcgtag tggctgctaa tttctgctcc
    27241 gctatcgacc aataacaggc gacccgaagt aagcatttgg gaatggtcgt ggttatgaag
    27301 ggtttcgcca ttctgtgaca gaatgattgg gaacgaaggc atgtatcctt gagcaattgc
    27361 aacaccctca atagccccag taattgcctg ttcactgatt ccgggcttgg ccatacgcat
    27421 ggcggttacg tgcatctggt aacctacctc gcatgctttt tcaatctcgg caatttcaac
    27481 atcttcctta actgaacgaa ggcttacaat ggctttaatc agttcaacgg atgaatagtt
    27541 tttaatgacc gattggttaa ttcctagcag ctgttcaagc agtaaaacat tatcggcacg
    27601 gtaaggggga aggaaatgga ttcggcgtcc gtgtttaatg gcgcggctta aagtagagta
    27661 aagctccttg aaagggtgag ttgaaaggat accaaccttt tcgcccagct ccttcagggt
    27721 tggctggggg cccatccaaa taatatcatc gatgtcaaaa tcgttgccgt ataaacaatc
    27781 ttttccgtct tcaatatcaa taacacccac taaccccgga atgtcgagac caaagaaata
    27841 gagaaaatta ctatcctgac ggaaacggta ggtgtttgac ttataattca ttgggctttc
    27901 ggtgttccca aggataatca ccagacctga tttaatcttt ttccgtagct ttaatctgcg
    27961 ttctacgtaa acttgctttt cgaacatagc tgttgatttt ttatggttgc tgtaaatgta
    28021 agcaccaaaa ccctatttaa caagagaaaa aatcaatata gtgttaaagg gtttggcgta
    28081 tattgcaaat ctacataaag taaaagcaaa aaacagtata ttcggaaatt gtagtttaac
    28141 caacaatcat aactgcctgt tgataagaag agtaagataa atgatagttt cgcataggca
    28201 taggtttatt ttcattaaat cggccaaaac tgccagtaca agcgttgagc taatgctaag
    28261 caaaatatgt ggcctcgatg atgttttaac gcccctggct ccttttgatg agcgattcga
    28321 cgccgattac tatgaatacc aacccaggaa ttgcgaggga tttcagaacc atatgtcggc
    28381 aattgcaatt aaaaacaagg ttggcgaaga ggtttggaac caatacctta agattaccat
    28441 tgtccgaaat ccttgggata tggttgtttc acgttatcac tgggaaaagg ctcgcctatg
    28501 gcccagtatc gggaaaaatg ttgctaacat tttgagagaa ccctttgcca tagtaaggta
    28561 taaaaggtta ctacacagga taaaattgat ttttgtgcta tcatcgttta gcaatttcat
    28621 acgatacttt gataaaacct ggaccaactc aaggttttac tttgatgagg aagggaagcc
    28681 aatttgcgat gtttacctta ggttcgaaaa tcttaacaaa ggcataaatg acctcgaaaa
    28741 aatgctaggg atacagctgg gagacctgcc acgcctaaag agtaagcccc gaaaaggcaa
    28801 aaaacactat agccagtact ttaaccaaca acaaaaggaa agagttgaaa aactctttaa
    28861 acacgaaatt gaagtttttg gttatacctt tgagcaagta aaccagtaga gcaaaatgtt
    28921 agcaaaaacc tatcaactca ttgtaaagta taagaattca ttgatgtggg cgctggtaat
    28981 tcttatccta tgcggaatgc caccaagcgg tttgcccacc attaaaatac ccggcattga
    29041 taaaatggtt cattttggat tatttgccgt atttgccctt ttacttatct cagaaatgaa
    29101 ctaccagagg gttcagctca cggttaaaaa tcggcataaa tgggtgggtt ttattgtagc
    29161 ctcggtttac ggtggtttga tagagttgac acagcaatac ctttttacgt cgcgcggtgc
    29221 tgagtgggcc gatttttttg ccgatattct tggtgctgca gttgctgttc tggtttatcc
    29281 ttatatcaat aagaccctaa agggatatat ttagaaatag gtaaacgaaa aaagcgcccc
    29341 aaagggcgct tttagtattt attggacgct atgccattac tttagaacca attcggtata
    29401 accaatcatg tagccatcca tgtaaagggt gattttgtaa gtgcccttag ccagctcgcc
    29461 gctactgtcg aagaaaatac acatgtcaac atccttattc tggtaatcaa cctcacggtt
    29521 agccgagaat acaatctttt cgccctcaaa atcgaaaagg tcggtttccg attttgcaag
    29581 gacaaaatcg tcggggccta ctacgcgcat gtaaacatag cgtgagcctg gtttggcaat
    29641 agcattctca ttaagagtaa agcaggtttt gatcttagcc gtacggctag cgcgggatgt
    29701 ttcgcttccc cttctattga gagcaacagc tgttacattg cgtgctttaa ccacggaacc
    29761 ctttgccacc tgggtattta gctcctcggt gcgctgctca agctccttaa cggttttttt
    29821 agccgtggct gcctcttcct tcaccttaat attctcagca ataagttgct ggttaagggt
    29881 attcaatgag tctatttcgc gaaccatgtt cttcatgata gccctaagag ttccgagctc
    29941 cttttggtac tccttgattt tagcgtagct aacgcgcttg aggttttgaa gctccgagta
    30001 aagcttttgt gccctctcct gctccatttg aagcttgcgg ttcaatgaat cgttgtcggt
    30061 ttccaaatcc ttgtagtcgt tcatcaggtt aagcaagttg ttgctgagcg aatccttttc
    30121 ggcaagcaac atggtttcgg tttcctttgc ctcctttttt tgctggtagt aaacgtacga
    30181 aaggccggca attacaacta gcagaatgac aataagtacc gataggaacg atactgcttt
    30241 tgatgatttt gtgctttcca tatcttgttt tttacgtttg ggttactatt gttaacggtt
    30301 atttggctcg gttagtgtaa ggttttgaaa aacgtcctcc aagttaagca ctttttgctg
    30361 aagtgttaac agggtaagat tgttgttctt ggcaaagtta aatattgttg gcctaatatc
    30421 ggtatttgac gacgatgaaa ccagaaaagt ggttgcagtt agttgattta cgtttgaaac
    30481 attctcgata ctctgaagaa gttcaacgga tggcgcttcc aggaattcca caacaacgtt
    30541 ttggaccgac gatgaggtaa gcgcttttat ctccgaagca ggtccatcgg caacgatact
    30601 acccttgttg ataatgatta ttcgcccgca tattgcctcc acctcctgca tgatatgggt
    30661 tgaaaggatt acggttttat tttgacccac cgcggcaatc agatttcgaa tttcaacaat
    30721 ttgattgggg tcaaggccgc tggtgggctc atcgaggatt aaaaccgatg ggttgtgaat
    30781 aagggcttgg gccaagccca ccctctgacg gtaacccttt gagaggctgc caatcttttt
    30841 attttgctcg tgtcctatac cggtaatatc aataatctcc aaaacacgtt tacgggcatt
    30901 tttacccaaa cggtaaattc cggcaaccca caaaaggtat tccttaacgt acatatcggt
    30961 gtagagcggg ttgtgttctg gcaggtaacc aatatcgcgg cggaaatcgt aggcaggcga
    31021 gttgatgtca acaccgttaa caagtgcaga gccgctatcg ggtgtaaggt agccggtgat
    31081 aatcttcatt aaggtagatt tgcccgcacc atttggcccc aaaaggccaa caacctgccc
    31141 ctgtggaacc tgaaaggtaa cgctgtttaa agccacctgc ttgccaaact gcttggtgat
    31201 actgtttact gtaattgaca tcagcaatgt tgatttttac tcaatgttgt ttccataagg
    31261 tttttggcca tgttgagcgc agcgaatatc atgttcatgg taataaaagc aatatccttc
    31321 gcattactca ggacgcaatt aggttaaaag agagccgtta accaagccat accttattga
    31381 atacaaacat agtaaaattg caggaaaaga aaattgtaaa aaatgtttaa aattatgaac
    31441 ttagcatgat atttgaatct tacgctgtag ccatggttaa ccctaaaact atcgatatga
    31501 aaaagttttc gtcgttcata attgcagtgc tagcaggcac tgtaacccat gcacaaagtg
    31561 catcagaatc aataaatact tttgcgtttg acctgtacag gggcctaaag gttggaggta
    31621 acgaaaacct ggtttactca ccgttcagca ttacacccgc atttggaatg gttacgcttg
    31681 gagccagggg cgaaacactt aaccagctga acgctacctt tcattttggc aatagcccaa
    31741 agtttcatca ggcagttggt aagcttcagc aaagtttaat taagtcgtcg tcggctgaca
    31801 ttactataaa tgttgccaat aaggcatggc tgcaaaccga ttacaagata cttaaaaggt
    31861 ataggtgcga cctgaagggc aactataaaa caagcctgta taaggttgat tttattaatg
    31921 accccgaggc tgcccgtaaa actatcaacc atgccgtgga gtacgatacg caacgccaca
    31981 tcaaggattt gctaccacag ggtagtatta gcaatttaac ccgacttgtg cttaccaatg
    32041 cggtttactt taagggaaag tggaaggaaa cctttgaacc cgaaaaaacc aaggagcgcg
    32101 actttaccct ggccgatggc tcaaaaacca agcatcagtt tatgagcgcc gacaaaacct
    32161 ttggttacct caaaggaacc gactttgctg ccctggaaat ggactataag ggcgaagagc
    32221 tatcgatgct gctaatactg cccaacgagg gaaaaccgct gagtgaattt gagaaagcat
    32281 ttaccgttga aaagtataac gaggtggtaa aggcaataga accccaaaaa accctggtgt
    32341 ttataccacg gttcaccgtt gagagcggct tctcaatgaa gaaagtactt ggcgagatgg
    32401 gtttaaccat accctttagc gatgatgccg atttttccgg aatatcgggg aataaggatt
    32461 taaagataag cgatgcttac cataaagcct ttattgaggt atcggaagaa ggaacaaccg
    32521 cagcagcggc tactgccgta gtggtggcca tgaaatcgat gcccaacttc aacgtttttg
    32581 atgccaacag gccattcatg tttattttaa ggcataaacc aaccagtacc attctgttta
    32641 taggaagggt ggcaaaacca tagtgctagc tgaatagttt tgtaaagttc gcacttgtca
    32701 aaataagaca agtgcgaaac gttttttagc agtttacctc agtagttgtt gcggcataca
    32761 ctactccgcc tgggagcctt cattgaatgt agaaaaaatt ccccttgttt atgcacagcc
    32821 ccaaaaacct ttttatgtct ggctgatatg tagagtttag cttatgctca ccccctactt
    32881 tgcttgcacg cgtccacaga cgagtgctaa caaggggata gtttgtaaaa gagaataatc
    32941 atgcaagtgg aattcaccca aataaaacaa gttgttgtac attaagtttt attttctgga
    33001 ttcctccaaa attatttgct caatgagtaa tttgttttca ggaaggtaat tggtcgcaat
    33061 atcccaaata atttcataat caattccgaa atattcatgt gataccttat tcctcaaata
    33121 atacatctct cgccaaggta tttttgggta tttttcttta acgaaatcgg gaagattttt
    33181 agatgcttct ccaattattt caaagtttct aattactgcg tcaatggttt tataatctcg
    33241 cttaaaagag ataaaatcca tacctgcaat atattctgaa attctagaaa ttgatagctg
    33301 gatatcttca agatacatta agtatgtgcg tttgtccttg tccacactat acgtaattta
    33361 ctttgttaag aatatcctct tttaatctat cgcgaagagc atttttggtg actaaatcta
    33421 tctttcggcc aaaaatttgt tctaagtaga tttctaatga gaagaatttc cagcctatgg
    33481 gtttttctag ttcaataagt aaatcaatat cgctatcatc tctagcgctg tcagatgcaa
    33541 aggaaccgaa taaaccaatt tcttttacag agtattccct cttcaagaat ggtttcaact
    33601 ctcttaactt atttagtata tcatccctat tcaacatatt gcaaatatac taatcacaag
    33661 attcaaatca ataagtgtaa acaatttctt acatgcggca caacagccgt gggtgccaaa
    33721 gacaaagtgc tagctaaata gttttgtaaa gttagcactt gtcatttttt tgacaagagc
    33781 gaaacgtttt cagctgtttt cttcagtttt tgttacggca tacgctactc cgcctgggag
    33841 ccttcattga atgtagaaaa aaaatcccct agtttacgca cgtccaaaaa aaccttttta
    33901 tgtctggctg tagcgtagca tttagcttgt gctctccccc aaacatgccc gcacgcgtca
    33961 acagaggcgc gctaacaaga gggtaggctt ggagttacaa atgtttcacc cctacggggc
    34021 tagaagcgtt gctcgttgtt cgtgaatcgt tgttcgtgaa tatctttgtt taacagggtg
    34081 ttacattgtg aaacgctgtg atacttgctg ttgcttggtt tatgttgtga ttgccatgct
    34141 gagagctgta gaagcatcgc ttttcccttt catccccttt aatgggattc ctcgactagc
    34201 tcggaatgac aacacaattg gctccctcac tcactttgtc atgctgagct agccgaagca
    34261 tcacattgta cctttattcc cttaatacct tttatcgttc tttgacatgc tcttctgttg
    34321 cgatgcaaag cttcagtgta tctttttggc atatgcattg cgtgcatcgc aatacatatg
    34381 ggggtaggct tggagttaca aatattacgc ccctacgggg ctagaagcgt tgttcgttgt
    34441 tcgtgaatcg ttgttcgtga atatctttgt ttaacagcgt gttacattgt gaaacgctgt
    34501 gatacttgct gttgcttggt ttatgttgtg attgccatgc tgagagctgt agaagcatcg
    34561 cttttccctt tcatcccctt taatgggatt cctcgactag ctcggaatga caacacaatt
    34621 ggctccctca ctcactttgt catgctgagc tagccgaagc atcacattgt acctttattc
    34681 ccttaatacc ttttatcgtt ctttgacatg ctcttctgtt gcgatgcaaa gcttcagtgt
    34741 atctttttgg catatgcatt gcgtgcatcg caatacatat gggggtaggc ttggagttac
    34801 aaatattacg cccctacggg gctggaatcg ttgctcgtga atcgttgttc gttgttggta
    34861 aatgcaattc tgtgtaacac cgtgttacac cgtgtaaccc tatgaaacat atttagttga
    34921 tggttgacag ttgatggttg ttagtgaggg taatgtcatg ttgagcgtta gcgaaaaatc
    34981 actttagggg cgattagagt tccttcgctg cgctcagaat gacaagcgga gttggcagtt
    35041 gatggttgat gggtgatagt gcatagaagc atttcacgaa acactaaaaa ccaaacacta
    35101 ctttccatct tccctgtaaa ctgcacactg tacactgcac actattcaat agatccctcg
    35161 actacgctcg ggatgacaaa tgtagttgac agttgacagt tgatagtgaa aatactaaac
    35221 actaaaaacc aaacactaaa aacagttctg gggcaacttg gttcttctgg ctgatttatt
    35281 gcgcttaggc taggggttac tgctaaggct taccttctcg ttaagggtta caagcttgtc
    35341 gctaatatcc ttaacctgtt ggtttacctg ctttacaagt ttggagtagg tttcctgggt
    35401 tatgtttttg ccatcgagca ggctgatggc tacctttagc tcatcgagct ggtgggccag
    35461 cctttcggtt tcaatccgta gctcgtcgtt tttggatgtt tcaggtaccc gtgctaacct
    35521 attgagctgg aactggaata tggagtaaaa aacctcggtt tggcgcgcta cggaatcgga
    35581 gcgggtaaaa agattcgata gctcagtgtt tttcatgttt tgggtggctg cttgctgcct
    35641 ggagcagagt tccggacgca gcttgcaaat caaatcgatg tttgcatcgg cctggcgctg
    35701 aatggcaagc agtttctcgt tgaagttctt caccatggac aggtctttac cgcttaaatc
    35761 gatttttaga aaatcacgta tgttgaacac atcgcgttca atctcagtaa ttttcccgtt
    35821 caggaaacgt gcgctttcaa ggtttgcgtt atcggggtac aaatgcttaa agttttgctg
    35881 catgagctga atgagctcaa tgcgattaaa aacccatggt tgaagagccg ttgcaatgct
    35941 gtcgtagcgc tgaagttcta ctttaaggta gtatgcatcg agcctttggg taaactccgt
    36001 tactgcgcca agggaacgct cgcggcaaag cgagcaagag gttgacttgg caagccggag
    36061 taggttatcg gtaatggttt tgtttttggg atttgcctcg gctttccgaa ccctttcaat
    36121 ttcggcctca aaggttccct tgaaaagttg ctcctccagc cttgagaggt ggttcgcgat
    36181 gatttgaccc tcgaggtttt ggctcatcag ctccggctta aagatatcgg agaaccttag
    36241 gtcggcaatg tatttttcca ggtcggcatt gggtactttg ggatcggcct cgaggcgaac
    36301 aagggtgtta acaaggttct tggtcgattt taggttgtgg tcgttatcca tgtaaagcga
    36361 ggcaaagccg aggattgatt cgtactcctg gttgaaaacg gttgaaaaag tattattgcc
    36421 ccgatactta tacttcatct cctcataggc tttttgtagc tgacggtagt actgcataac
    36481 ccggaaaaga ctgaccgaaa tggcatcgtt attcccggtt cgatagttta ggctgtaaat
    36541 gtcgaggtga ttttttagct gggcaaaaat gttgaggaaa ttgagtaaat ccttttcctg
    36601 gcggtaagtt tcggcattga taagtccctg gcggaagtcg ttctcaacct taagtttacg
    36661 cttttcgata ttggcaaagg ttacctcgtc gcccgaaatt tgttcggtgc ggtataacga
    36721 tttgtagttt gccactacaa ttacagggta gctccggatg ccaataagtt cccgcagctg
    36781 gttacggtta acccgcccat ttgtaacggt atcgaggaat tgacgaagtg gtacaccaga
    36841 aaactcaagt gcaggcgtgt ttgcagtaga aagcaagtaa agccttatgg agtgtaggcg
    36901 ggtatcgaaa ttcttctgct caaacaacct gatggtagag ctaaaccggt actccttttt
    36961 cttggtgtta gcctcaataa aattgccaaa ctcgcctata atgctcataa cggcatcggt
    37021 tggagtggta atctttgaaa ggtttttcag ctgcgaggca acaagcgcaa gcatttggtc
    37081 gcggtcgtta ttggtgatgg cacgaacacg tatttccgca tcgatgtaat cgcgggccga
    37141 gtataagggc aggttatcga taatgcggac ataatcaata ttatcgctta caaaggtttg
    37201 gtacttgcta tccttgtcgc gtgaaatctg gaagttatag agggggtagg ttatatcgtt
    37261 atcgccaaaa aactttacgt tcttaaggct agcggtaata aagaggtact caagttgctc
    37321 ctcctccaca cccagctttt tgcgcctttt tttcttgggt tcaacccttg caaaatcgag
    37381 ctcattgatg agcgtactga acttatcgcc atttagcaga aagatgtcgg ttgtgaggta
    37441 ctgccactcg cctgtgaagt tgaagcttgc cgtatcggag aaaagcagcc ttggttcaat
    37501 atcgcgctgg ccaaaggcaa gtaatgccga tagccaaagc aaaccgataa aagcgataga
    37561 tttggtgttc atacttaaga tttcaaattg aaaaggataa aggttataag gataaaggtt
    37621 ataaggataa aggataaagg ttaaaggata aagggttaaa agatatattt aaaaaatcaa
    37681 ttctttctct ctatattttt tttactaact catttagttc ttccactttt ggttttagtt
    37741 gaagatactc aatgtaccaa catgtaattt catttggatt catagcacaa cctttaaacc
    37801 tccaaatcct ttgtatttac taattttttc aatatcttct ttcgtttttt ctctttcatt
    37861 acctgtaata tcatagtaag ttcttattgc ttcaaggttg tcctcaaaca cttgtctttt
    37921 attgtaactc atacactata attttaattt aattacttcc atcatttcat cttcaaactc
    37981 atcggtatat gcaaaatcaa tcttttcaaa cccgtacttc tcaaatattc cctctcctgc
    38041 tttcactatt tcaattatct ttttctttaa cgttccttca ctttccaatc tttcataata
    38101 atcttcatcc atttctctta tcctttcctc aatgtatgag tattttgaat tagtatatcc
    38161 ctcttttaaa acttctattg ctccgagttc tgccattaat ggcatttctc ccctttcaat
    38221 catatcatta tataatctaa atgccatttc cgtttttgcc ctcaaaaagt cttcgtactc
    38281 tattcccata ttttccttta tttctgggaa aaaatcaacc atgtactcat caaggtattt
    38341 cctaaaattt tctttagttt ccattttttc tactttcttg ttgaacaaat ttaaactttt
    38401 actatctact aacaaccatc accaaaccta cctagctgac cctacgggga gggacaacct
    38461 ccttggtggg gtcgaacatg tagcggtagg ttatgtgtgt tttacgcacc cgagcaccaa
    38521 tttgttcggc cacgcgtcgc atggtggggt taaaatcgcc aatccagttc agctccagct
    38581 cggtgtaacg gaatccaggg gctaaagccc gcttagcgaa ttcatttacc agcgcaccct
    38641 caactccctt tccctggtgc tttggaacaa taccaaaaat caggccaatg gccttggtgc
    38701 aaaccttttt aactttaagt aagtacaaaa agtacaactt ggaaaggata tcgaacttac
    38761 cgttcaggtg ccttacaacc tgattaaggt cgggtaccat gataaaaaat ccaatgggtt
    38821 cgccctcgta gtaagcaaag tacatgagct cggggtcaat aataggcttc atggttttca
    38881 ttagagccat ggcgtgagcc tcagtaattt ttttaacacc ggtgtatcgg ccccaagcgt
    38941 tgttataaat ggtgcggaaa tcctcggcaa acttcttcaa attctttttc gatattgttg
    39001 taatggtgta cgaagggtta cgagcaatac gctcggcctt ttcccaaaca accgggtcaa
    39061 cattttcaga gttaactaac cggtggtagg tgtactggtg gaagtaaacc ttaaatccat
    39121 agttttcgaa gaggttgcgg tagtatggca agtggtaatc catgcagtag ttgggcggga
    39181 aatcgccatc aaccagcagg ccccaccagc gatcgcggtc gccaaagtta atggggccat
    39241 ccatggcctc catgcctttt gatttgagcc actcccgggc agtatcgaac atcaggttag
    39301 ccgcctcctg gctattgatg cactcaaaaa atcctatgcc acccgtaggc tgctcagagt
    39361 ttttggcaat ctctttatcg tagaaagcag caattcggcc aaccggttta ccgttatcgt
    39421 ataaaaccca gcggatagcc tcgccatggc ggaaatgctt gttaacctcg gggttgaaaa
    39481 cattctcaat atccatgtca agcgggcgta tccagtttgg ataatcgctg taaagcgaaa
    39541 gcggtagttc aagaaaatcg ttacgggtgt gtttactgtt tacctctaca agtttgaaat
    39601 tagccatgac agttaaattg gtttactacg tatcagcatt tcagcaatct gtaaatcgaa
    39661 aggagttgta attttaatgt tttcccggtt accctcaacg gtatgtatgg tgtatccctg
    39721 cgattctact actgaggcat cgtcggtaaa gtagggttgc tcaggaacgt tatagcattc
    39781 cttaatcagc tccgatttaa aaacctgagg ggtttgcaca agtaaaacgc tattacggtc
    39841 aaatgcttta gaggtactgc cctcaaccag gcgcaccgat tcaacaggtt tgattacggg
    39901 aattgcaata cctttttggg ctgcagttgc atagcactcc tcaataacct gttttgaaac
    39961 aagtggccga acgccatcgt gaatggcgac aagggagttg tttaaaggga ttagggaaag
    40021 accattttta accgactgaa agcgagtttc acccccggca gtaagggtta aatttgcggt
    40081 aaaattatac tcaacgcaaa ggtttttcca tcgctccatc tggtttttgg ggagaacaag
    40141 cacaataccg ggtttttggg gaagggacaa aaaaagctca atggtatgca ttagaatggg
    40201 cttgccgttc aggatcagga actgcttggg ggcttcggaa cccattcgtt gacccgagcc
    40261 accggcaaca ataaccgcaa aacgattagt gctcataaag taagattatc ggctccgaaa
    40321 gttatcaaaa attatcccaa gtgcaatgac ttaaggggtt aattcgggat gagtcggagt
    40381 ggaaggaata tgagggagtt atagtccgtt aaacgttaaa cgtgagccgt taagcgcgtg
    40441 aataaagtta gaatgagtag taatatgaag aagttagagg aagttagagg aagacgaatc
    40501 atgaatcgtt aaacgtaacc gttaaaagtt aaccgttaaa agtaaaagcg tgaataaaag
    40561 tcggaatgag tagtaatata aagaagttag aagaagttag aggaatatga atcgtgaacc
    40621 gttaatcgtt aaaagttaaa catgtgaata tgatggagtt agaagaagtt agaggaagac
    40681 gaatcaagaa tatttaaacg taaccgttaa atgtgaaccg ttaaatgtaa aagcgtgaat
    40741 aaaagtcgga atgagctgga atggtagaat gtgagggaag taaatgtaga gaaaataaaa
    40801 aaaacatctt tccgaaaacg tttgcgggtt tattaaacca taaaacaatg gcaaaatgtt
    40861 gattttcaac aattgaatgc tgatatttgc ggggatgcat ggtaaaaaca acatatatgg
    40921 ttaagtacaa ctatgtaaag gagtttgttg agaaacccat taggaaacac tgggaactca
    40981 actcgctatc ggattttaaa ggggaaacct ttacctatgc cgatgtagcc cgaaaaatac
    41041 actatctcca taaactatat caaatatggg gtgtaaaaac tggcgataag gttgcccttg
    41101 tgggaaggaa taatgctgag tggggcatag tgtatctttc cgtaatatca tatggggctg
    41161 ttattgtgcc aatactaccg gatttttcgc caaccgagat acacaacatt gtaaaccatt
    41221 ccgattctgt gcttcttttt gcaggcgata tggtttgggg cggactgaac caagccgaaa
    41281 tgcccaacct aaaagcaata ttcagcattt cagggtttaa cctccttgtt tcaaatgatg
    41341 aggaaagccg aaggctattt gaggctgcaa aatcctcaat taccgatgag atgaaaagtc
    41401 tcacccccga caaggttaac tatcccgaag taccaaacag tgagcttggg gttatcagtt
    41461 acacatcggg gacaactgga ttctcaaagg gagtaatgct gccacttaac agtctggcag
    41521 ccaataccga ttatgcccga aataacatgc acctggtaaa gggcgatcaa attgtatcgt
    41581 ttttaccgct ggcacacgca tttggatgtg cttttgaatt tctattccca tttgccatgg
    41641 ggtgccatat tacgttttta accaaaatcc cgtcgccccc tataattctt gaggcatttg
    41701 ccaaggttcg cccacgctta gtgctgactg tgcccctggt aatagaaaag attttcaaga
    41761 aacaagtaca gcctaagatt gaaaagcccg ccatgaaggt tttactcaac attccattgc
    41821 taaataaggt aatatttaag aagattaacg agaaactatc aaatgcattt ggtggcaatt
    41881 ttcatgagat agtaataggt ggtgcagcgc taaaccccga tgtagaacgc ttcctacgtc
    41941 gtattaaatt caagtataca gttgggtatg ggatgacaga atgcggcccg cttatttcgt
    42001 attcggggtg gcgtaccaat cccatgggct cgtgcggcaa ggtgataaac taccttgaga
    42061 tgaaaataga ttcatcggac ccatatagcg aggttggcga aattctggta aggggtgaga
    42121 acgtaatgat gggctactac aaaaaccccg aagccaccca aaaggcgttg gacaatggct
    42181 ggctacacac tggcgatttg ggggtatacg acagtaaagg ttttatatac ataaaaggcc
    42241 gaagtaaaag catgatactt ggcccaagtg gtcaaaacat ttaccccgag gagattgagg
    42301 cacggcttaa taatattcct tttgttcagg aatcgcttgt gatagagcaa aagggtaaac
    42361 ttattgggtt gatttacccc gattacgagt cggttcagca gcaaggtatt gaccccgata
    42421 agctggagcc ggttcttgaa caccatcgcc ttgaggtaaa caagcatcta ccccagtata
    42481 gccaaatagc caaacttgtg gttcacaacc aggagtttga gaaaactccc aaaaagagca
    42541 ttaaacggtt caaataccag tactccgaca actagttgtg ttccgataat agtaaaaccc
    42601 ggagcattat taggtaaagt attggaagca taacggtttg taaaaaccta aagaaaaaaa
    42661 agttgagtat accgcggtaa atgagaatac ctagaagggt aataatagaa caatcgagca
    42721 gggccaggaa aaccctgctt gtttttttat gaaaaacttt tggcagcaag tgctgtaggc
    42781 taaccccgga catgtggtaa aggcaaatgg taagaataac aggggtaata attccgaaaa
    42841 ccagaagaat aaaaggcgat tgtaaaatcc tgaatgagta tagaatacca aaagataaaa
    42901 agagaaaaag attgaacgaa aacgcaccag gcttttccac caggttgcgg gtccagaaat
    42961 gcagtatatg ctctttggct tgctgattca taatgagtta ggctttgata atttgataag
    43021 caagatacgc catgtaaacc atcacaagaa tcaccccctc gcgtcggtcg aacttacgac
    43081 ctgggccact aactgcaaag actaacatta gaattccagc agcaatgttt agggctaaat
    43141 cgaggtttgc accactgtat agcttaatgg ggtttattcc ggcagtaagg cccaatatta
    43201 gcaagatatt gaaaatgttt gaacctacca cattgccaat ggcaatatcg ggtttgttgc
    43261 ggtatgcagc cacaaccgag gtggccagct caggtagcga tgttcccgca gctacaatgg
    43321 ttaaacctat aattgactgg cttaccccaa gttttgtggc catattaaca gccccaagca
    43381 caataagttt accgcctcct accagaaatg ctaaccccca aataaccatc agcaccgaca
    43441 tccaaagttt tcgggtttca atttcgggcg aggctgaggc tggatcctcc tttccaacca
    43501 ggaaattgta gtacatgaaa acacaaaaaa gtaaaagtaa aaccaagcct gccgaccgcg
    43561 atattacatt tagctctgct gaatcgagga aaacctcgtt tgagagcacc catagcacta
    43621 aagccgagag taaaaagaaa ggaatttcaa cctttacggt gctatgctgt acaccaatcg
    43681 gtttaaccag ggcagctaaa cccagaatca taaaagtatt gaaaatgtta cttcccaaaa
    43741 tattgccaag ggcaatatcg gaattaccct ctatagccga aataaggtta actgataatt
    43801 ccggagccga ggtaccaaaa gcaaccactg ttaggccaat ggttaatgct gatataccca
    43861 aacgctttgc tatactcgat gcgccctcaa caagccatgt agcacctaat acaaggatta
    43921 ctaccccaag aataataaat aaaagattca tcagttttaa gtttgaagta aacagacagg
    43981 ttgatttaaa cccaataaca atataaaaat aaggttaatc agcgcaaaga tacaatagat
    44041 taggttaact ttgctcgaag tttaacctat aatagtatag ccttgaaaag gattttaatt
    44101 acagttggcg cttcggtttt ggttttactt atgtttcctc ggtgtgccaa ggtggtttcg
    44161 ccaaccggtg ggccaaagga tactattccc ccggttttag tcaatagtaa tccaaaacta
    44221 aacgcaaccg ggtttagcgg acaaaaaatt gttctggaat ttgatgagtt tattcagctc
    44281 aaggagttac agcaaaaact gttggtatcg ccacctctaa aaagtaaacc ccaacccaag
    44341 cagaaaggca agaaactaga gcttgagctt accgacaccc taaaacccaa taccacatat
    44401 accttttact tttccgatgc agtaagggac aacaatgaag gtaacccgat taaaaatttt
    44461 atattctcat tctcaaccgg gcaaacaatc gataccctta ccctttcagg acgtgtattg
    44521 aatgcaatta ctaacgagcc cgttgaaaac gcccttgtaa tgctttacga ttcatttacc
    44581 gataccctac cctacactac tctacccaat catattgcaa aaaccgataa ggacggaaaa
    44641 tacacattgg taaacctaaa gcccataaag tacaagcttg tggttgttga cgataaaaac
    44701 ggcaattacc tatataacca gggttcggag agtattggtt ttatttctta tgaagtaaac
    44761 ccatccgata aaaaccccaa aagtatcagt ataagactgt tcagggagga actccccaac
    44821 caaatagtaa cagggtacga taggcctgaa cgcaacatac tctccttggg cttttctcga
    44881 aaacctgttg gtgggtttag cctgaaacct gttgataggc ttaaattaaa gaactggtat
    44941 gtggttgaac ccgataacaa tggcgatacg cttaaacttt ggatagtaaa tgatgagata
    45001 gccaatcgtg atacccttat ggccatactt gaataccaaa aaactgactc gttatacagg
    45061 cttaaaccac aaaccgatac attacgattc ctttactaca aggaagaggt tcaacagtcc
    45121 aagagtaaag gtaagaaaga tgaagccgtc caggtaaaga aaggttttac ggttagcacc
    45181 tctttgccct ccgataggat ggtaataccg aatgtgccat ttgaactcac acttcccata
    45241 cccattcgga aggcagataa caccaaaata tcgattttca ataccaccga cagtatagct
    45301 gacgagcaga ttaaattgaa ggtagatagc ctgaacccaa ggattttcag atttgaaaag
    45361 gaatggaaac ccaatacctc gtacaagctc accattttac ctggaacttt tgaagactat
    45421 acaggtcaaa ccaacgatac cctaatgctg gaaatgattg gtgccgaccc tgaaaagtat
    45481 ggtaccctaa gaatcaacct aactggcgta agtaagtcag ctgtggttga attgctgagc
    45541 gaaaagggca tacctgttga tgcaagaacc attcagggga atggaatagt agaattcagc
    45601 tttgttaagc cgggtaaata taggttacgg tttattgatg actctaacaa taacgggaaa
    45661 tgggattcgg gtagctacat gaagcgaatt cagcccgaaa gggttattta ctttaccgat
    45721 gaaaaaacaa agggagttat aaatatcagg gcaaactggg atagcgagtt acagttctca
    45781 ttaaaacagg aataagcaat gtcagtacta atgaattttg ccatgttccc aaccgataaa
    45841 ggggcaagtg ttagccccta cgtggctaag attattgagt tgataggcgg gttgggttat
    45901 gaattcaggc ttacggccat gggaaccata gttgaaaccc ccacccttca gcaggccctc
    45961 gatgtggttg ataaagccta taagctactt gagcccgatt gtgaaagggt ttatgtgagc
    46021 atcaacctcg atatcagaaa aagtgattta ggccggattg atgggaaagt taaatcggta
    46081 acagaaaagt taaaaaaaag taaccagtag catagcatga taaaaaaaag tctgacagta
    46141 ttactcacca tttttgccct atcggcatat agtcaaacct atgttggggt aaagggggga
    46201 tacaccatga gcgatattgc tttctcgccc gttcagaata cccgtatgct ttttgccgat
    46261 ggattcgatt acggactggt ttttaagtac tttaacctga aatattttgg tttacaggct
    46321 gagctttaca tggtaaacag gggttaccgt aagcctattg atttggcaga gctgggcgat
    46381 acgctataca aacgcgtaaa cacatacatt gaactcccca tctttatgca ggttaggttt
    46441 aaccttaagc ttttcatgat tcatttcaat gccggaccct atattgcata cctggttcag
    46501 gcgcgcgaag gcgataactc atcgggtgaa taccagatga gcaatatctc cattaacata
    46561 cttcgcgata atcgtataga ttttggtgta cttggaggta ttggcttaag ccgcgatttt
    46621 aagtggggaa ctttttttgc agaggggcga gttggatatg gttatggcga tatgcaaaag
    46681 catacctata caggaatgcc aaagcagtca aaagcagtat ttcaaagcgt aaacgtaggc
    46741 tacctgtatc attttggaaa taacgtaaaa taaggataaa acaggcgaac ctttttagcc
    46801 atcagcatgt ttaggttcaa aagttatttt gatgaaaagc tggaacgaaa taccttacga
    46861 tatacagcgt gtaatttcta ataccgcagt agcccctggc gcatatattc tgaagtttaa
    46921 aaaaaactac ccctttattg ccggccaagt gcttgccttg ggtgtttcca aggaaataga
    46981 accacggtat tatagcattg cctcgcccga aaacgacgag ctgatatcga tactttatac
    47041 tgaaagggtt gaagggaaac taacacccat gctatcgctc ttaaaaccag gcgaaaacat
    47101 aatggtttcc aaaccatttg gaacctttac tcatgtcaag ccttcgcccg tattcattgc
    47161 agcaggaaca ggaattgccc cattcatgtc gatgttgctt acgggtaagg caagcggagt
    47221 taccctcata catggcgcgc cctaccccga atacttttac tactccgata ctctaataag
    47281 taggcttggc gaaaactata tccgatgctg ctcacgttgc cagcccggat gcctgtttgt
    47341 ggggcgcgta actgactttg tgaaacagtg gaacgacctg gatgtcaata ggccctacta
    47401 cctttgcggt agcgccgaga tggttgtgga aacccgcgat atactgatat cgagaggcgt
    47461 tcctgtgagc aatataaatt cggagatata cttttagctg atggttccac tttacggaaa
    47521 aacgctcgat gagcttaaat cgatagtaac tgccgaaggg cttccgtcgt ttacggcatc
    47581 ccaaatagcc gattggcttt accatcacca tgccgatagt atcgatgaga tgaccaatct
    47641 atcgaaaaag gccagggaga tgctttcagc aaagtatacc gtagggttat ccaaacccga
    47701 aagggtttcg aaatcggccg atggtaccaa aaagtatctg tttccggtag ccaataaccg
    47761 cttcgttgag gctgcctata ttcctgatga tgaccgcgca accctttgcg tttcttcgca
    47821 atcgggctgt aagatgggat gcctgttttg catgactgca aagcagggat ttcaaggaaa
    47881 tctttcggct ggtgaaatac tgaaccagct acgcagcatc cctgagttta aaaatgtgac
    47941 aaacattgtg tacatgggga tgggcgaacc actcgataac ctcgagaatg ttcttaaaag
    48001 ccttgaaatt cttacctcaa agtggggaat gggctggagc accaagcgca ttacggtttc
    48061 aaccattggt cttatccctg ccatgaagca gttccttgaa aagtcaaact gccacttagc
    48121 cattagcctt catactccgt tcgatgatga gcgccggatg cttatgccta tccagaatgt
    48181 atacccaata gccgatgtgg ttgacgagct caaaaagtac cgatttagcg accaccacag
    48241 ggtgtcgttt gagtatataa tgtttgatgg gctaaacgat accccgcgcc acgtaaagga
    48301 actggtgaag ctgcttaatg ggataaactg ccggataaat ctaatccgat tccatcccat
    48361 acccgattcg ccgctccgca cctcacccga cgagcgcatt atacagtttc gcgattcact
    48421 aaccgccaaa ggcatattta ccacaatccg tgcctcgcgt gggcaggaca ttcaggctgc
    48481 ttgtggattg ttatcaacca aggcgctaca ggaaaaacgt taaccgttaa ccgtgagccg
    48541 ttaaccgtta accgtgagtc gttaagcgaa atctaattat tacctattgt cattccgagc
    48601 tcgtcgagga atctcaatta cgagttaaat cgggaaacgt atgccttgag ccgtaaagcg
    48661 aagtagaaca gcaactacaa cgcttgtcat cctgagcctg ttgaaggatc tcccctttat
    48721 ggtaagggag atgtttcgct aatgctcaac atgacaatac cctcacgaac aact
//
LOCUS       DAOOWJ010000005        44445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_898_length_44445_cov_7.466299, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000005 DAOOWJ010000000
VERSION     DAOOWJ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 44445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44445
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_898_length_44445_cov_7.466299"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..2586
                     /locus_tag="PLW22_01065"
     CDS             295..2586
                     /locus_tag="PLW22_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU73320.1"
                     /translation="MTNYLDELSEVQREAVTCTEGPSLVIASAGSGKTRVLTYRIAHL
                     LTLGVKPWSVLALTFTNKAAREMKERIARIVGHETAAKLWMGTFHSIFAKILRNEAEL
                     LGYSKNFTIYDTTDSKNLIAQIIKELKLDVQVYKPGEILSRISIAKNNLITPQAYAQN
                     ASIVSQDISSRKGEIHRIYTLYSQRCQKASAMDFDDLLLNTNILFRDFPDVLQKYQNK
                     FRYILVDEYQDTNFSQYLIVKKLSESHRNLCVVGDDAQSIYSFRGAKIENILNFQNDY
                     KDYKLFKLEQNYRSTQTIVNAANSVIAKNQRQIPKVAYSSNEVGEPIKLIKAFTDQEE
                     GFLVASEIVSLVHQAHCSYSDIAILYRTNAQSRIFEETLRKRNIPYRIYGSLSFYQRK
                     EIKDLIAYIRLTVNPNDDEAFNRVVNYPARGIGDTTMEHLQALANQLGLSLWQTVGEL
                     GKHNGGVKPAAAKKLEEFRNLIVSFQQKLYTMDAYEAVHTIVNSSGMLTDLKSDKSQE
                     GISRLQNIDEMLNGIREFIESRKEEGFEETVTLVDYLENVALLTDQDTDKEGDTKCVS
                     VMTVHSAKGLEFDYVFLTGLEDNLFPSTLNVKSPEDLEEERRLFYVALTRAAKRAYIT
                     YAQTRYRWGSPTHCTPSRFIKEIDSEYIDVPFDEGDHGNIAERPYSKGQSFSGFTSQS
                     SALAAPSYTKPNQQVPTTPKNTRDISPEAITVGMSVYHAKFGEGVVESIEGAGPNRKA
                     LVNFNLVGSKNLLLKFANLKQLE"
     gene            2994..3320
                     /locus_tag="PLW22_01070"
     CDS             2994..3320
                     /locus_tag="PLW22_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU73321.1"
                     /translation="MPDHIHIFIGYNVNQLIPNLVENIKTSSNAWEKKEEKLSKYKFE
                     WQRGYEAFSHSRSQLDTVVKYIQNQEQHQKKSFRNEYLEILRKIDIKYQYEYLFEFFE
                     NGGVWD"
     gene            3549..4139
                     /locus_tag="PLW22_01075"
     CDS             3549..4139
                     /locus_tag="PLW22_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOU73322.1"
                     /translation="MDYNTQRKKLRLPEYGRHIQQMVDHIASIDDREERNRLAKSLIA
                     IMGNLNPQLRAVNDFRHKLWDHLFIMSDFRLDIESPYPKPDLEKYNEKPRRVPYPSHP
                     INFKHYGRIIELMIEKAIEMDEGPEKEAFKQLIANQMKKANITWNKESVTDDDIFRDM
                     EHLSKGRLVMAPGSKLIDKREVKPTGGKRPKHHRKR"
     gene            4154..5461
                     /gene="murA"
                     /locus_tag="PLW22_01080"
     CDS             4154..5461
                     /gene="murA"
                     /locus_tag="PLW22_01080"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU73323.1"
                     /translation="MATFEVYGGKPLKGELHPQGAKNEALQIICAALLTAEPVTIKNI
                     PNIRDVNKLIELLQGMGVEVQRHSSSECTFTAKNVDVEYLTTNDFKVKAAAIRGSIMV
                     VGPLLARYGRAYAPKPGGDKIGRRRLDTHFLGFQKLGATFNFDTRENFFTLEGKNMRG
                     TYMLLDEASVTGTANILMAAVLTPGKTTIYNAACEPYLQQLSKMLVSMGAKISGIGSN
                     LLTVEGVESLGSCTHTILPDMIEIGSFIGLAALTASDITIRNVSYPNLGIIPDSFRRM
                     GIEFEVIGDDIHIPPQEHYEIDTFIDGSILTIADAPWPGLTPDLLSVFLVVATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGHDRKMKLRPTVMTSPDIRAGV
                     ALLIAALSAEGKSVIHNIEQIDRGYENIDGRLNAIGANIRRTD"
     gene            5549..6934
                     /locus_tag="PLW22_01085"
     CDS             5549..6934
                     /locus_tag="PLW22_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU73324.1"
                     /translation="MLMRFWSKISILIIFLAGVGFTSRAQLNAPGRVITLLTQYSNTV
                     NQDSIFVFYGDIGKLSARHTTGNIANFKWYRYNPLVSNPTLRFELFAEEVSVVQSEQL
                     NLNEGGYRVVITDIADSTETFTCWLFTDNVSLDRIDIFNSCQFLELNSITIPSSYNVA
                     YDRFTYHDLSRSNQPERNTYGQQYFANVTWQASESSIELPSSSALKLVIENPAPLHNS
                     TYTVTIQNPFGRTLTLTTPEVTAVATKADNLIQVDERGVWNDFDANAEYEALLGLRLE
                     SKSLNCDSIYWRITTRKISPESIFDISVWRDSSLIAQRNEAFPDKKVMVPGFYKVYHY
                     SVNTLSGCIDSVITDIKVDSSRISADAIPNVFSPNGDGKNDVFRFSDTDESIRSIRSF
                     TIRIYSRSGKLVYTYSGNPSEWKGWDGRTSSGSDAAEGVYYYIIEARGWDDRRFARGP
                     YKGFLHLFRGR"
     gene            complement(7231..7689)
                     /locus_tag="PLW22_01090"
     CDS             complement(7231..7689)
                     /locus_tag="PLW22_01090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU73325.1"
                     /translation="MRTAEIHTSKGVMKVKFYEEDAPNTVANFVKLANEGFYDGLAFH
                     RVIPNFVIQGGCPFSKDMTDPRVGTGGPGYKINCELTGNNQYHDRGVLSMAHAGRNTG
                     GSQFFICHNRQNTKHLDRQHTCFGKVYEGLEVIDAIRVGDRIEKIVIVEE"
     gene            complement(7772..9928)
                     /locus_tag="PLW22_01095"
     CDS             complement(7772..9928)
                     /locus_tag="PLW22_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU73326.1"
                     /translation="MKTYQTEQIRNIALLGNTGSGKTSLAESMMFEGKIIERKGTIDG
                     KNTVSDYTEIEQQNQRSIFSTLLYTEFNNSKLNIIDTPGSDDFVGAVISSMYPTDLLV
                     MVINAQYGVEVGTEIFNRHAEHLHKPMILAVNQLDHDKANFDATLDSLKQTFGNKVIL
                     TQYPVATGAAFDSFIDVITMKMYKFKGDTGIREEFPIPDSEMDKATELHNALIEAAAE
                     NDESLMEKFFEQGSLSEDEMRSGLSKGIASRSIMPVFCLSAAKNIGTKRLMDFIINVG
                     PNPTHSPVLKNNNNKPITCDASAPTSLFIFKSTVEPHIGEISYFRVMSGKVTEGMDLT
                     NANNSTKERLSQLFVVAGKNRNKVTELVAGDIGATVKLKNSKTNHTLCGPGLDFTFDP
                     MEFPDPKFRTAVKPKNEAEVEKLGELLNRAHQEDPTILVEHSKELRQIILSGQGEFHL
                     NILKWQLTNLHKLEIEYIAPRIPYRETITKVAQADYRHKKQSGGAGQFGEVHLVIEPY
                     VEGAPDPVKYKVNGKEINLSVRGKEVIDLEWGGKLVFYNCIVGGAIDARFMPAILKGI
                     MEKMEQGPLTGSYARDIRVAVYDGKMHPVDSNEISFKLAGRNAFKNAFKDAGPKIMEP
                     IYDVEVLVPADRMGDVMSDLQNRRAIIMGMGSVKGFEVINAKVPLAELNRYSTALSSL
                     TSGRATYTMKFASYEQVPADVQEKLLKAYQEQEEEE"
     gene            10333..10722
                     /gene="rpsL"
                     /locus_tag="PLW22_01100"
     CDS             10333..10722
                     /gene="rpsL"
                     /locus_tag="PLW22_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOU73327.1"
                     /translation="MPTIQQLVRKGRSKLVDKSKAPALDACPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHLIRGTL
                     DASGVEGRKQGRSKYGAKRPKDKASKK"
     gene            10752..11228
                     /gene="rpsG"
                     /locus_tag="PLW22_01105"
     CDS             10752..11228
                     /gene="rpsG"
                     /locus_tag="PLW22_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOU73328.1"
                     /translation="MRKAKPKKRIILPDPKFGDIMVTKFVNNLMVTGKKSIAFGIFYN
                     ALDIVGKRMKDSEKSPLEIWKIALENVTPQVEVKSRRVGGATFQVPTEVRPERKQSLS
                     MKNLILYARKRVGKGMSDKLAAEIMAAYNQEGGAFKRKEDMHKMAEANKAFAHFRF"
     gene            11260..13377
                     /gene="fusA"
                     /locus_tag="PLW22_01110"
     CDS             11260..13377
                     /gene="fusA"
                     /locus_tag="PLW22_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU73329.1"
                     /translation="MSRDLRFTRNIGIMAHIDAGKTTTTERILFYTGKTHRIGEVHDG
                     AATMDWMVQEQERGITITSAATTTFWNYENKQYQINIIDTPGHVDFTVEVERSLRVLD
                     GAIATFCAVGGVEPQSETVWRQADKYRVPRIAYVNKMDRVGADFLGVVQEIKDKLGAN
                     PIPIQLPIGAEDNFNGIIDLVENKAVVFFEDEKLKATNYRLEPVPANMKDEVDEWRAK
                     LIEAVASYNDELLERFFEDPDSITKQEIIETIRKATIDMSIIPVLCGSSFKNKGIQHL
                     LDAVIAFLPSPLDKGDVVGTNPNTGNEEVRKPYIDQPFCGLAFKIATDPFVGRLAFIR
                     VYSGKVDSGSYVFNTRTGKKERISRIYQMHANKQNPIEFCEAGDICAAVGFKDIRTGD
                     TLCLEEKPIALEAMTFPEPVIGLAIEPKTQKDLDKLGIALGKLSEEDPTFRVNTDPDS
                     GQTVISGMGELHLEIIVDRLRREFNVEINQGAPQVAYREAFTRSVSHREVFKKQTGGK
                     GKFADIIFELGPADEGVTGLQFIDEVKGGNIPREFIPSVQKGFELAMSNGILAGYAIP
                     SMKVVLKDGSFHPVDSDALSFEIAARYGFRAAGPKAAPVLLEPIMNVEVVTPEENMGD
                     VIGDLNKRRGQIAGMESKGNARVVKAKVPLAEMFGYVTVLRTLTSGRATSTMEFSHYA
                     EVPANIAKEVIEKSKGKELKEIE"
     gene            13400..13705
                     /gene="rpsJ"
                     /locus_tag="PLW22_01115"
     CDS             13400..13705
                     /gene="rpsJ"
                     /locus_tag="PLW22_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOU73330.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSYKRLLDIYSSNAKTIDALMKLELPSGVEVEIKV"
     gene            13731..14348
                     /gene="rplC"
                     /locus_tag="PLW22_01120"
     CDS             13731..14348
                     /gene="rplC"
                     /locus_tag="PLW22_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOU73331.1"
                     /translation="MPQGLIGRKIGMTSVFGEDGKNIPCTVIEAGPCVVTQVKNAETD
                     GYAAIQLAYDDKKEKHTSRPMLGHFKKANTTPKRKLVEFRDWEGLQLGDVVTVDLFAN
                     DNWVDVVGITKGKGFQGVVKRHGFNGVGGRTHGQHNRQRHPGSMGASSFPSRVLPGKR
                     LAGRTGGDRVKIINLRVLKVIPESNLLLVKGSIPGAKGSYIIIEK"
     gene            14348..14980
                     /gene="rplD"
                     /locus_tag="PLW22_01125"
     CDS             14348..14980
                     /gene="rplD"
                     /locus_tag="PLW22_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOU73332.1"
                     /translation="MEIKVLNIQGKETGRTIQLEDSIFGIEPNDHAIYLDVKQILANR
                     RQGTHKSKQRNEVSGSTRKLKRQKGTDTARAGSIKSPVFRGGGRIFGPVPRDYSFKLN
                     KKIKQLARKSALAYKVKENALLVIEDFSFDTPKTKEFVNICKNLNVADKKTLFLLGET
                     NKNIYLSSRNLQKQATAIASSVNTYEILNANMLVLTESSVDALNKMFKVS"
     gene            14993..15283
                     /gene="rplW"
                     /locus_tag="PLW22_01130"
     CDS             14993..15283
                     /gene="rplW"
                     /locus_tag="PLW22_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOU73333.1"
                     /translation="MDILIKPVVTEKMERLTDKLNQYGFIVDKRANKLQIKKAIEELY
                     GVTVDSVNTMRYAGKVKSRYTRTGVLVGRTNSYKKAIVTLKEGDKIDFYSNI"
     gene            15289..16113
                     /gene="rplB"
                     /locus_tag="PLW22_01135"
     CDS             15289..16113
                     /gene="rplB"
                     /locus_tag="PLW22_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOU73334.1"
                     /translation="MAVRKLKPVTPGQRHKIIGLFDDITTNKPEKSLLVPLKKTGGRN
                     NQGRRTMRYIGGGHKRRYRLIDFLRNRENEPAVVKTIEYDPNRTARIALVEYPDGEKR
                     YIVAPAGLKVGQTIMSGRGVAPEVGNTLYLGEIPLGTIIHNIELYPGRGACMARSAGS
                     YAQLLAREGKYAIIKLPSGETRMVLNECKATIGSVSNSDHALEKSGKAGRSRWLGRRP
                     RTRGVAMNPVDHPMGGGEGRASGGHPRSRKGIPAKGYKTRSKKKHSSKFIIERRKK"
     gene            16133..16402
                     /gene="rpsS"
                     /locus_tag="PLW22_01140"
     CDS             16133..16402
                     /gene="rpsS"
                     /locus_tag="PLW22_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOU73335.1"
                     /translation="MSRSLKKGPFIDYKLEKRVIQMNESNKKSVIKTWSRASMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRGHAGNKNK"
     gene            16426..16839
                     /gene="rplV"
                     /locus_tag="PLW22_01145"
     CDS             16426..16839
                     /gene="rplV"
                     /locus_tag="PLW22_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOU73336.1"
                     /translation="MGARKHNMAERLKAERKNKAVARLRNCPSSPRKMRLVVDLVRGK
                     DVNTALNILKFSRKDAAKDVHKLILSAVANWKAKNENVRIEDANLYIKEIFVDQGRML
                     KRIRTAPQGRAHRIRKRSNHVTVVVDSAVNNESNN"
     gene            16847..17584
                     /gene="rpsC"
                     /locus_tag="PLW22_01150"
     CDS             16847..17584
                     /gene="rpsC"
                     /locus_tag="PLW22_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOU73337.1"
                     /translation="MGQKVNPIANRLGIIRGWDSNWYGGKKYAQKLVEDSKIRDYLNA
                     RLAKASLSKIVIERTLKLITVTIHTARPGIIIGKGGQEVDKLREELRKITNKDVQINI
                     FEVKRPEIDAVIVGNNIARQIEGRISYRRAIKTAIASTMRMGAEGIKVQISGRLGGAE
                     MARSETYKEGRIPLHTFRADIDYALAEAHTKVGVIGIKTWICNGIVYGKRDLSPNIGT
                     KSVANVTEKKAKPAPKGGSRPRAKKKK"
     gene            17616..18044
                     /gene="rplP"
                     /locus_tag="PLW22_01155"
     CDS             17616..18044
                     /gene="rplP"
                     /locus_tag="PLW22_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOU73338.1"
                     /translation="MLQPKKTKYRRQQKGKMKGIAQRGNQLAFGSFGIKALESSWITS
                     RQIEAARQAVTRYMKREGQIWIRIFPDKPITKKPAEVRMGKGKGAPEGFVAPVTPGRI
                     LFECDGVPYEVAKEALRLAAQKLPITTKFVVRPDFVEPTN"
     gene            18052..18252
                     /gene="rpmC"
                     /locus_tag="PLW22_01160"
     CDS             18052..18252
                     /gene="rpmC"
                     /locus_tag="PLW22_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOU73339.1"
                     /translation="MKVAEIRELTDKEILERIELEKTNLLKLRLNHSISPLDNPMKIK
                     ETRRNIARLQTVLNQRKLSQNK"
     gene            18265..18537
                     /gene="rpsQ"
                     /locus_tag="PLW22_01165"
     CDS             18265..18537
                     /gene="rpsQ"
                     /locus_tag="PLW22_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOU73340.1"
                     /translation="MEQVNEVKRNNRKESIGVVVSNKMQKSIVVAVKRKVKHPIYGKF
                     VNKTTKFYAHDENNVCEVGDLVRIMETRPLSKLKRWRLVEIIEKAK"
     gene            18540..18905
                     /gene="rplN"
                     /locus_tag="PLW22_01170"
     CDS             18540..18905
                     /gene="rplN"
                     /locus_tag="PLW22_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOU73341.1"
                     /translation="MIQQESRLVVADNSGAKEVLCIRVLGGTGKRYARIGDKIVVTVK
                     SAIPGGDIKKGTVSKAVVVRTKKEIRRQDGSYIRFDDNACVLLNNQGEIRGTRIFGPV
                     ARELRDNYMKIVSLAPEVL"
     gene            18926..19246
                     /gene="rplX"
                     /locus_tag="PLW22_01175"
     CDS             18926..19246
                     /gene="rplX"
                     /locus_tag="PLW22_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOU73342.1"
                     /translation="MAKLHIKKGDMVYVISGESKGHQGRVLSVLVKENRAIVEGANMV
                     SRHTKPNSKNPQGGIIKKEAPIHISNLMLVDPATGKPTRIGRRLNDQGKLVRYSKKSG
                     EEIK"
     gene            19246..19803
                     /gene="rplE"
                     /locus_tag="PLW22_01180"
     CDS             19246..19803
                     /gene="rplE"
                     /locus_tag="PLW22_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOU73343.1"
                     /translation="MEYVPTLKKKYREEIVPALMKQFNYKSVMQVPRLEKIVINQGIG
                     QAVADRKLIEQAQAELTMIAGQKAIQTYSRKDISNFKLRKGVPIGLKVTLRRERMYEF
                     LERLINVSLPRIRDFKGINDKFDGRGNYTLGIQEQIIFPEIDIDKVSKILGVEITFVT
                     TSENDEEAYALLREFGLPFKNAKKE"
     gene            19807..20076
                     /gene="rpsN"
                     /locus_tag="PLW22_01185"
     CDS             19807..20076
                     /gene="rpsN"
                     /locus_tag="PLW22_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOU73344.1"
                     /translation="MAKESMKAREVRRAKLVAKYAEKRARLKEEGDYVGLQKLPRNSN
                     PIRLRNRCMLTGRPRGYMRQFGISRITFREMASKGLIPGVKKASW"
     gene            20102..20497
                     /gene="rpsH"
                     /locus_tag="PLW22_01190"
     CDS             20102..20497
                     /gene="rpsH"
                     /locus_tag="PLW22_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOU73345.1"
                     /translation="MTDPIADYLTRVRNAVMARHRVVEVPASNMKKDITKILFDKGYI
                     LNYKFEDDGKQGIIKIALKYHPETKACAIKHLERVSTPGLRRYSNAKDLPRVLNGLGI
                     AIISTSQGLMTDKEARAKNIGGEVVCYVY"
     gene            20519..21073
                     /gene="rplF"
                     /locus_tag="PLW22_01195"
     CDS             20519..21073
                     /gene="rplF"
                     /locus_tag="PLW22_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOU73346.1"
                     /translation="MSRIGKKPVNLPQGVSVNISADNVVSVKGPLGELKQKVDPDITV
                     KVEGNEVVLSRPTDHKRHRSLHGLYRALINNMVIGVSKGFEVQQELVGVGYKAEAKGQ
                     ILEMSLGFSHDIYFEMPKEIKVETITEKKGNPKIILKSIDKQLLGQVAAKIRSLRKPE
                     PYKGKGIRFVGEVVRKKAGKSASV"
     gene            21096..21455
                     /gene="rplR"
                     /locus_tag="PLW22_01200"
     CDS             21096..21455
                     /gene="rplR"
                     /locus_tag="PLW22_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOU73347.1"
                     /translation="MALNKLERKLRIRRRIRKRVSGTAQKPRMSVFRSNTNIYVQFID
                     DINGVTLASASSMSKEIAEKKNITKTEQARLVGQLAAKNAMAKGIESVVFDRGGNLYH
                     GRVKALADAAREGGLKF"
     gene            21461..21982
                     /gene="rpsE"
                     /locus_tag="PLW22_01205"
     CDS             21461..21982
                     /gene="rpsE"
                     /locus_tag="PLW22_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOU73348.1"
                     /translation="MSQNTNIRRVKASDLELKDRLVSIQRVTKVTKGGRTFSFSAIVV
                     VGNENGVVGYGLGKASEVTAAIAKGIEDAKKNLVKVPILKGTIPHEQEAKFGGAKVLI
                     KPASHGTGVKAGGAMRAVLESVGVTDVLAKSKGSSNPHNLVKATVMALLELRDAHTVA
                     QQRGVDLKKVFNG"
     gene            21995..22174
                     /gene="rpmD"
                     /locus_tag="PLW22_01210"
     CDS             21995..22174
                     /gene="rpmD"
                     /locus_tag="PLW22_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOU73349.1"
                     /translation="MAKIKVTQIRSVIGRPEGQRLTLRSLGIRRMHHTVEVEATPQVM
                     GMIAKVRHLVRIEEQ"
     gene            22201..22647
                     /gene="rplO"
                     /locus_tag="PLW22_01215"
     CDS             22201..22647
                     /gene="rplO"
                     /locus_tag="PLW22_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOU73350.1"
                     /translation="MKLDKLKPAEGSTHNRKRIARGEGSGHGGTATRGHKGAKSRSGY
                     SRKFGFEGGQMPIQRRLPKFGFKNPFRVEYKPINLEVLQALAEKHGYTTIDIDTLINA
                     GLVSRNDMVKILAKGTLSAKLEVKAHAFSKKAIEAIEAANGTAVKL"
     gene            22654..23970
                     /gene="secY"
                     /locus_tag="PLW22_01220"
     CDS             22654..23970
                     /gene="secY"
                     /locus_tag="PLW22_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU73351.1"
                     /translation="MKAFFETLKNIYKIEDLRKRILYTLGILLIYRLGSFIVIPGLDP
                     SQLGALQEQTKEGIMGLLDMFSGGAFHNASIFALGIMPYISASIVVQLLGIAVPYFQR
                     LQKEGESGRNKLNQITRVLTVGILLFQGPAYITNLYIQLPQGAFLLSKFWFTITATIT
                     MIGGTMFIMWLGEKITDKGIGNGISLIIMIGIIARLPFAFVAELFSKAEELGGGLVML
                     LIELVILFAVFMFSILLIQGTRKIPVQYAKRIVGNKQYGGVRQYIPLKVNAAGVMPII
                     FAQALMFIPMLFARFDATAGIAAAFSYTGFLYNFTFGLLIIVFTYFYTAIIINPNQMA
                     EDMKKNGGFIPGVKPGKKTVEYLDSIMSKITLPGSIFLAIIAILPAFAIKLGVNNQFA
                     QFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKSGRLKGRSGM"
     gene            24000..24776
                     /gene="map"
                     /locus_tag="PLW22_01225"
     CDS             24000..24776
                     /gene="map"
                     /locus_tag="PLW22_01225"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOU73352.1"
                     /translation="MFLAKTDEEVELLRLNNDLVSRTLAEVAKHIKPGVTTQKLDSIA
                     EEFIRAHGAVPGFLGYNGYPATLCISVNDAVVHGIPSSYELREGDIVSVDCGTFLHGF
                     YGDSAFTFPVGEVKPEVQRLLKVTRECLELGIEAAVAGGRVGDISYAVQSHAESNGYS
                     VVRELVGHGIGRHMHEKPEVPNYGARGRGPKLVDGMAICIEPMINLGSKHVYQDLDGW
                     TIRTRDGKPSAHFELAVVVRKDKAERLSTFKYIDEVLRLS"
     gene            24791..25009
                     /gene="infA"
                     /locus_tag="PLW22_01230"
     CDS             24791..25009
                     /gene="infA"
                     /locus_tag="PLW22_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOU73353.1"
                     /translation="MSKQTAIEKDGTIIEALSNAMFRVELDNGHVITAHISGKMRMHY
                     IKILPGDKVRVEMSPYDLTKGRITFRYK"
     gene            25029..25145
                     /gene="ykgO"
                     /locus_tag="PLW22_01235"
     CDS             25029..25145
                     /gene="ykgO"
                     /locus_tag="PLW22_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOU73354.1"
                     /translation="MKVRASVKKRSDECKIVRRKGRLYVINKKNPKFKQRQG"
     gene            25183..25560
                     /gene="rpsM"
                     /locus_tag="PLW22_01240"
     CDS             25183..25560
                     /gene="rpsM"
                     /locus_tag="PLW22_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOU73355.1"
                     /translation="MARIVGVDLPKNKRGEVALTYIYGIGRSSARKILDKAGISYDTK
                     VADWTDDNVTAIRNILNEGYKVEGELRSMVQLSIKRLMDIGCYRGIRHRLGLPVRGQS
                     TKNNARTRKGKRKTVANKKKATK"
     gene            25571..25957
                     /gene="rpsK"
                     /locus_tag="PLW22_01245"
     CDS             25571..25957
                     /gene="rpsK"
                     /locus_tag="PLW22_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOU73356.1"
                     /translation="MAKKTATKKKVVKVDPVGQAHVHSSFNNIIVTMTNSTGQVISWS
                     SAGKMGFKGSKKNTPYAGQTAAADCAKVAYDMGLRKVKVFVKGPGAGRESAIRTINST
                     GIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            25992..26600
                     /gene="rpsD"
                     /locus_tag="PLW22_01250"
     CDS             25992..26600
                     /gene="rpsD"
                     /locus_tag="PLW22_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOU73357.1"
                     /translation="MARYTGPTTKIARKFGEPIYGPDKYFEKKNYPPGVHGLMKKRKK
                     VSEYGTQLLEKQKAKYTYGLLERQFANLFKQAARSKGITGEILLQLLESRLDNVVYRL
                     GIAPSRAAARQLVLHKHIVVNGKVVNVPSFRLRPGDVVGVRERSKTLEAITDSLQSNR
                     HIKYSWLEWNNDKMEGKFMNVPERSEIPENIKEQLIVELYSK"
     gene            26616..27608
                     /locus_tag="PLW22_01255"
     CDS             26616..27608
                     /locus_tag="PLW22_01255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOU73358.1"
                     /translation="MAILAFQKPDKVIMLESTDNFGKFEFRPLEPGYGITVGNALRRI
                     LLSSLEGYAITWIKIRGVDHEFSTIPGVMEDVTEIVLNLKQVRFKRIVDGVDNEKVTI
                     TITGQNEFKAGYLSNFLSGFKVLNPELVICRMEPDVKIEIEVNISKGRGYVPAEENKP
                     EDAEFGIIPIDSIHTPIRNVKYSIENYRVEQKTDYEKLIIEISTDGSIHPKDALKEAA
                     KILIYHFMLFSDEKITLETEDKFETEEFDEEILHMRQLLKQKLVDLDLSVRALNCLKA
                     ADVETLGELVRYNKNDLLKFRNFGKKSLTELEDLLDSLNLSFGMDVSKYKLDKD"
     gene            27614..28138
                     /gene="rplQ"
                     /locus_tag="PLW22_01260"
     CDS             27614..28138
                     /gene="rplQ"
                     /locus_tag="PLW22_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOU73359.1"
                     /translation="MRHNKKFNHLGRKSAHRKAMLSNMASSLILHKRITTTVAKAKAL
                     RSYVEPLITRSKDDSTHQRRVVFSHLQNKQAVTELFREVAQKIGDRPGGYTRILKTGT
                     RIGDAADMCIIELVDFNTTYVKETAKPAAKKTRRAGSKAKKAETGSANVVAEEPKSEN
                     QDSENATSTDEKAE"
     gene            28224..29516
                     /gene="eno"
                     /locus_tag="PLW22_01265"
     CDS             28224..29516
                     /gene="eno"
                     /locus_tag="PLW22_01265"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOU73360.1"
                     /translation="MGQIVSVHAREILDSRGNPTVEVDVITESGYFGRAAVPSGASTG
                     VHEAVELRDGDKGRYLGKGVLKAVNNVNTIIADEIVGMNVLDQQLIDETMIKLDGTPN
                     KGNLGANAILGVSLAVAHAAAQLTNQPLYRYVGGVNARTLPIPMMNILNGGSHADNSI
                     DFQEFMIMPVGANSFTEAIRMGAEVFHNLKSVLKKLGYSTNVGDEGGFAPNLKSNEEA
                     ITVILQAIENAGYRPGEDIYIALDPASSEYYLPEEKVYHLHKSTGEKLTPAQMVDYWK
                     NWVDKYPIISIEDGMAEDDWDGWKLITQTLGNRIQLVGDDLFVTNVNRLQKGIDTGVG
                     NSILIKVNQIGTLTETINAVRLADVHSMTSIMSHRSGETEDTTIAHLAVALNTGLIKT
                     GSASRSDRIAKYNQLIRIEEMLGDAAIYPGKNFKYIKK"
     gene            29598..31487
                     /locus_tag="PLW22_01270"
     CDS             29598..31487
                     /locus_tag="PLW22_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOU73361.1"
                     /translation="MAYKSLSHFIDVLEQNGELLRVKRFVDPVLEITEITDRHSKLPG
                     GGKALLFENTGTNFPVLTNALGSERRICLALGTNNLEALRDDVGRLLEQFTRPRGSLW
                     SKISALPELTRVASWMPASRTGKGICQQVVHYNPHLDMLPVLKCWPHDGGRFITLPMV
                     HTVDPNTGTRNVGMYRMQIFDSSHTGMHWHKHKTGAKHFEEYKRLGKLMPIAVTLGGD
                     PAYTYAATAPMPENVDEYILAGFLRKKRVKLVKCLTVDIEVPEDVDFVIEGYVDPSEE
                     PAWEGPFGDHTGFYSLADWYPRLKVTCITHRADAVYPATIVGVPPMEDAYIAKATERI
                     FLEPIRRTLLPELTDMVLPTEGVAHNIALVKIKDEFQGQAYRVMNALWGAGQMMFNKV
                     LVAFPNNINLTDGYQMLNHICRTVNPQSDLFFGKGPLDVLDHSSNQFAFGSKLFIDAT
                     ALNYRETGYSISIDDIKSSTLAFSNHIVALNLTLIEKDIPILIASVRKKEIEDWQLFK
                     QELTLQPIFQDLFALVLVDLENQIDDLSLVLWFLSGNIDPSRDLSITRVNGSNLILID
                     GTRKAYPTDNFPRLWPNIVTMDGETIERVDQMWHWLDIGEFIESPSLKFFNLNQTDDA
                     VMRIQ"
     gene            31512..34844
                     /locus_tag="PLW22_01275"
     CDS             31512..34844
                     /locus_tag="PLW22_01275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOU73362.1"
                     /translation="MGLFRTDKKLLPSCVAALLLAATSSVFAQRVNVTLFNSDNGLPQ
                     SFVNALLQDSLGFLWIGTQDGLCRYDGYDFVVFNNNPYDSLSLSNNYVNHLVEGVNGE
                     LWIATRSGLNRVNRKTGKVKVYYNHANNPRSLSSNMILRVYRDRSNRIWVKTIDALHL
                     YNPTTDDFTRFPHFNDIFNISQTSDLSPIFENSNERLWVGTKDGLFYFDKERLVFKRY
                     SEDPANPNSIWGNRVNAITEGLSGKLLIGTDKGINEFDFLTQKFRRVILTLNDPSNKS
                     LTTGIQFLGYDNDSSLWVGSVGGLGKLLPNGTFIKYNSIYSNNLPVNILNVSTVLRDR
                     TGVLWIGSQTGLVKVNPFEHRIGGYSKDPNGRNLFSNNVAASVIESTDGNIWVGTWGS
                     GLHIFNPTTGENRHYWSGGGNYIPNDFIHSLYQTRDGRVLVGTRNGVFQYLPSSRQFV
                     DFFERNNVYVGDLFKNNRVYSITEDLQDRVWFATRQGLHCLKGGNLISFYARRGDSLS
                     LPGNEVYDVVADKNANVWVATISGLCRISADLRTIETFSFIGNKSRLPFEVLCLYIAK
                     NETVWVGTTSGLFWINPKGKNVLHSVVLPDINIRLINDILEDDRGRFWISTNKGLVLY
                     NPLSNLVKVFNNSDGLFSNELNINASFKNSKGRMYFGSIDGVNVFHPDSIPTNKVVPN
                     VCITHIQVYSKSGNTEIIPYQLKFLRIPDDFSNLTFTIAALDFVQPNKNSYRYQLVGH
                     DSRWVELGNTNKITFSNLSEGDYELRIMGSNSDQVWTTEYKSLRIRAVAPWWRSRIAK
                     WIYGVIVLISLLVWAIRRNKHLREINRLLHERETVLEELRLQKDELAFKNKNITDSIN
                     YAKRIQESMLPSISRFKEFVPESFVIFKPKDIVSGDFYWVNETRNKTFIAVVDCTGHG
                     VPGAFMSIIGIELLRNITNVEGVDDAAEILNRLSVAIHNTFATSSFDSAEGVKVKDGM
                     DVSFCVIDKEYNMLQFAGAFSNLFLVRDGKLMEIKGDRYSVGMANELGHSQFSSYYIP
                     IQPNDTIYMFTDGIVDQFGGPEGKKFKYRRFRHLLLSNYMKPIELQRTNIEKAINDWM
                     GNLEQVDDMLIIGIKPELSCMF"
     gene            complement(34893..36323)
                     /locus_tag="PLW22_01280"
     CDS             complement(34893..36323)
                     /locus_tag="PLW22_01280"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOU73363.1"
                     /translation="MLLRFTTLTALFFAYTLAWAQQSNSLFFMHRIPQSNLINPAVQI
                     DCPVYVGVPFLSSLHLNLNSTGFSYNDIANGTGTINFPALVGQMHSWDYLSEELHYTP
                     VSFGFMYDNDQYFNFAWTERVESKLFVSKRLMSLFANGNTQFVGNGLITSNPGLNSFY
                     YREFSFGYSTRLRGDVLVGAHAKILFGLAGLYTKRNSVDIEIDALTHNIDARWNPQVN
                     VAYPLGVTTDAAGNVTGVSAGAFSPLPFFLNFSNPGLAFDLGFVKPNDPITWSGSILD
                     LGFIWWFKSTSRFTNDGHFVYRGATPADLTNPDAYIDMLADSINNQVQFQHSNGSFVS
                     FLNPKAYFGGTYAFTPQLLAGAHVRTEWYPGRPVVGLTLSAIAMAKKGSSVSLTYSVM
                     NGSFANIGAAINLGGESFQFYILTDNLLAPFYPERVRNANLRFGFNLFWGCKQKKKKL
                     SIPESSGGGCFWTWGLDQKRKQHGVK"
     gene            complement(36327..36680)
                     /locus_tag="PLW22_01285"
     CDS             complement(36327..36680)
                     /locus_tag="PLW22_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73364.1"
                     /translation="MIRVNIWNEFPLQGNAQIWILDNGQNKLDSLFAQPASVPAAEIY
                     SNGNVVGTKFESYQVDFDQNRIDLWQNAATAVVRAGLVITEEGVTEQNIQYFDRYKLK
                     VQLAARVDFKTIKNN"
     gene            37717..37995
                     /locus_tag="PLW22_01290"
     CDS             37717..37995
                     /locus_tag="PLW22_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73365.1"
                     /translation="MAGFKFFSIPKPKGFRYKPIYYNEQKEEMREREERVRKELGLTD
                     KDKPFVPSIRGQFQRARKISRPSSRQSNIRLLIIIAILLLISYFVFFR"
     gene            37997..39829
                     /gene="mutL"
                     /locus_tag="PLW22_01295"
     CDS             37997..39829
                     /gene="mutL"
                     /locus_tag="PLW22_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOU73366.1"
                     /translation="MADVIQLLPDSIANQIAAGEVVQRPASVVKELLENSIDAGARNI
                     NLIVREGGKGLIQVIDDGVGMSETDARLCFERHATSKIREAADLFNIRTMGFRGEALA
                     SIAAVAEVELRTRRAVDEVGTEVNISASELVSQQPVACSTGTQIAVKNLFFNVPARRK
                     FLKSDSVELKHIITEFQRVALAYPEVSFSLSHNGSELYRLPASGLKQRVIGLFGKTIN
                     QHLVDCSTQTSIVNIGGYIGKPEGAKKTAGEQFFFVNGRFMKNPFLHKAVMNAYNRLL
                     PPDTYPSYFIYFDIDPQSIDVNIHPTKTEIKFEDERMIWQILHAAVRESLGKFSVVPP
                     IDFDSAPGFDIPYLPKNAPVTFPTIDVDPTYNPFDEHNRNVRKPHSADKLRESATGWE
                     QLFTDLPQDPTPIQTRIETFESEVMLSKAPENTNRFYQFKGKYILTTVKSGLMVIDQK
                     RAHERILYEQYISGMQADINLKQRELFPQAIELLPADFELLMSKADELSRLGMEISNL
                     NHNTISVNSMPVSSSVTDPAKLVENLLAIMHENQALPFDDAKHRIALTMAKASAIGYG
                     KQLSQFEMQEIVDKLFACHEPNLTPDGKKIIVILELDDIDSRFK"
     gene            39883..40707
                     /locus_tag="PLW22_01300"
     CDS             39883..40707
                     /locus_tag="PLW22_01300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU73367.1"
                     /translation="MIFGNRNIFSSTPPVVMNLIMINVVMLVVTWVMASTFGIDLNRI
                     LGVYPPGSPDFRPYQIVTHMFMHGGLLHLFFNMFALWMFGRILEQVWGSRRFFIYYMV
                     TGLGAVTIHLLVNYLHISAIQNDAMAMLNTPSPDAFAAFVTRHFPEYYDQVYAQFLDK
                     WYLFPNSPVYADQAAEYTRQLIALQQGIPTVGASGAVYGVLLAFGVLFPNTILMLLFP
                     PIPIKAKWMVIIYGGLELYLGLTQPGSSIAHFAHLGGMIFGFILIKLWQRRTDVFY"
     gene            40789..41664
                     /locus_tag="PLW22_01305"
     CDS             40789..41664
                     /locus_tag="PLW22_01305"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU73368.1"
                     /translation="MTIWDEIKSSFRLGSNLTRLIYINLGVFLLFGLLRVFLFLSASS
                     SGWITGFMAVPANLQSLMVRPWTIVTYMFYHEDFLHILFNLLWLYWFGQLFLQFFAQR
                     QLTWVYLLGGISGALLYILAFNLLPPFKGYAEVSLALGASASVLAVVIAVATLQPNFT
                     VYLLFLGAVRLKYLALATVVIDIISIPVSNAGGHIAHLGGALFGFLYVAALRSGTDLA
                     KPFYSISSIRFPKRNRMKVTYRSHNADYDYNYRKAQKQKQIDDILDKIARSGYDSLTK
                     EEKDILFKANNRNIN"
     gene            41664..42746
                     /locus_tag="PLW22_01310"
     CDS             41664..42746
                     /locus_tag="PLW22_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU73369.1"
                     /translation="MLIVRLIHRILVGVNVLFALLLVLTYVSVYINPEDFWFVALYGL
                     LYPFFLIVNLLFLFYWIFRWQKLVFISLAAILLGVSHFNGFIRFPYGSAAKGSNPDLR
                     VMTYNVNLFRLYSWSKLKPSYSDILTHIKDNDFDVVCLQEFYLREGKLSHLDVEQQSG
                     MKVYSSFILRRPSSAYGLAILTNQPVVSVGEISFPNSFNACMYADIVKGTDTIRVYNL
                     HLQSTRLKERNFNFLLRNEFKLDSKNYDELKDLLSRLGVAFTKRARQVNMVVEHMRTC
                     RYPIIICGDFNDSPVSYTYHTLTASLHDTFKDAGSGVVVTFSGLWPSYRIDYVLRSRQ
                     FRTVTYQSPRLRYSDHFPVVVELQLQ"
     gene            complement(42752..43717)
                     /locus_tag="PLW22_01315"
     CDS             complement(42752..43717)
                     /locus_tag="PLW22_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU73370.1"
                     /translation="MAMQDVTTPSHLDIENAFNLIAKHINSTPVLTSRSVNTILGCNA
                     YLKCENFQRVGAFKFRGATHAILRLGSDEAHNGVITHSSGNHAAALALAARQRGIPAY
                     IVMPENAPEIKKKAVAGYGGIITFCPPTQADREQTMLRIKSQTEATFIHPYDNFNVVC
                     GQGTAALELMQQEPELDIVIAPVGGGGLLSGTATFIKGRFPHVKVYGAEPKNADDAYR
                     SLRDHTIYPSVKPQTIADGLLTSLSPLTYTILSQHCDGILTVSEQSIIEAMRMVWERM
                     KIIIEPSSAVPVAAIMENAQLFRYKRVGVIISGGNVDLSHLPWQG"
     gene            complement(43723..>44445)
                     /locus_tag="PLW22_01320"
     CDS             complement(43723..>44445)
                     /locus_tag="PLW22_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73371.1"
                     /translation="PVPDSLRVINFRVTDYYSHEPIGLAHVMNITQKKGCISDLLGYF
                     SIPFKLGDSLSITAIGYHTKHVLNWGQFNKDTLFYELTLTPKVYQIEEVKISRFSTYD
                     RFLREFANLKLTKTRVTEQQEVIQLYFLKITKGLNLRNLPQPTMGLSFGKDWYQKQNE
                     KVAEAIEKDRKKRIADRKFNAGIVSSLTGLTGNELQEFMEYLKFDENFIITSTDYEIR
                     ERILDRFKEFETIKKGKNNTKK"
BASE COUNT        12301 a         9832 c        10388 g        11924 t
ORIGIN      
        1 actaatgagt tggcctttcc ttgtgccttc gtaattctaa tgacggatct gggttaattt
       61 ttccattttg gttatcaaaa ggtttttccc atactaaaag tataaaacaa aatcgatttt
      121 tacaagatgt aaccgtgccg atgtatttgg tggattgcag tttcgctatt aaccagtatc
      181 gaatttgaca ataaatatcg tttcattata aagcatgttg ataacaaact aatgatatgc
      241 ttgttgtaat ggtatgctac cactactttt gtggtaaatt tttttagagc tggaatgaca
      301 aactatttgg atgagttaag cgaggttcaa cgcgaggcgg ttacttgcac tgaggggccc
      361 agcttagtga ttgcttcggc aggttcaggt aaaacaaggg ttttaacata tcgcattgcc
      421 cacttactta ccctaggcgt taagccatgg tcggttttgg cgcttacttt taccaataaa
      481 gcagccaggg agatgaagga gcgtattgcc cgcattgtgg gtcatgagac tgctgctaag
      541 ctctggatgg gaacctttca ctcaatattt gctaaaatcc tgcgtaacga ggccgagtta
      601 cttggatata gcaagaattt caccatttac gacaccaccg actccaagaa cctgattgcc
      661 caaatcatta aggagcttaa gctcgatgtt caggtgtata agcccggcga gattctgtcg
      721 cgaatatcca ttgccaagaa caacctgata acgcctcaag cctacgcaca aaatgcctcc
      781 atcgtttcgc aggatattag ctcccgcaag ggtgaaatac atcgaattta caccctttac
      841 tcccagcgat gccaaaaggc ttcggctatg gactttgacg atctgctgct gaacaccaat
      901 atccttttcc gcgatttccc cgatgtgctt caaaagtacc agaataagtt ccgctatatt
      961 ctggttgatg agtatcagga caccaacttc tcgcagtatc tgattgtaaa aaagctttcc
     1021 gagagtcacc gtaacctttg cgtggtgggc gacgatgccc agagcattta ctcgttccgt
     1081 ggtgcaaaaa ttgagaatat cctcaacttc cagaacgatt acaaggacta taagctgttc
     1141 aagctggagc agaactaccg ttctacccaa accattgtaa atgctgcaaa cagcgtaatt
     1201 gccaagaacc agcgtcaaat accaaaggtg gcctattcca gtaacgaggt gggcgaacct
     1261 ataaagttga ttaaagcgtt taccgatcag gaggagggtt tccttgtggc ctccgagatt
     1321 gtgagcctgg tgcaccaggc ccattgcagc tatagcgata ttgccattct ttaccgaacc
     1381 aatgcccagt cgcgtatttt tgaggagacc ctgcgcaagc gcaacatacc ctatcgtatt
     1441 tacggtagcc tctcgtttta ccaacgtaag gagataaagg accttatagc ctacattcgc
     1501 cttaccgtaa accccaacga tgatgaagct tttaaccggg tggttaatta cccagcccgt
     1561 ggaataggcg acacaaccat ggagcacctt caggcccttg ccaaccagtt gggacttagc
     1621 ctttggcaaa ccgtgggtga actgggtaaa cataatggag gggttaaacc tgctgctgcc
     1681 aaaaagcttg aagagttcag gaatctgata gtatcgttcc aacaaaagct ctacactatg
     1741 gatgcctacg aggctgtgca taccatagtt aactcatcgg gtatgcttac cgaccttaag
     1801 tccgataaga gtcaggaagg catatcgcgc cttcagaata ttgatgagat gcttaacggt
     1861 atccgcgagt ttattgaaag tcgtaaagag gaagggttcg aggaaaccgt aaccctggtc
     1921 gattacctcg agaacgttgc cttgcttacc gaccaggaca ccgacaagga aggcgatacc
     1981 aaatgcgtta gcgtgatgac cgttcactcc gccaaagggc tggagttcga ttacgttttc
     2041 cttacaggac ttgaggataa ccttttccct tcaacattaa atgttaaatc acctgaggat
     2101 ttggaagagg agcgccgtct gttctacgtt gcacttaccc gcgctgccaa aagggcatat
     2161 atcacttatg cccaaacccg ctataggtgg ggatcgccaa cgcattgcac cccaagccga
     2221 tttatcaagg agattgattc ggagtatatt gacgtgccct ttgatgaggg cgaccatggc
     2281 aatatagccg aaaggccgta ctcaaagggg caaagcttca gtgggtttac tagccagtct
     2341 tccgcactgg ctgctccctc atacactaaa cctaaccagc aggtgccaac aaccccgaaa
     2401 aacaccaggg atatttcgcc cgaggccatt acagtgggaa tgtctgttta ccatgccaag
     2461 tttggcgaag gggttgtgga atcaattgaa ggagctggtc ctaaccggaa agctttagtc
     2521 aacttcaatt tggtgggttc taaaaattta ttgcttaaat ttgccaacct taagcagttg
     2581 gagtaaaaag ttttgctatc tacaaaagca tggcacctaa acattattct tctcctgagc
     2641 aaatttatta aaagatactc cagtgttcca ctgccctgca gcttgggggt ggaaccagtt
     2701 ccctcttccc caacaaatat tccgctgcgc tgcagctaaa ataggtgcgt agcaccgtaa
     2761 cctttgtacc caaagcacat gcaaaggaac cattagatgc gtagcaccga aacctttagt
     2821 taactttgaa gaaaaacaac tatggctaac acatacactc aattatacat acatctagtt
     2881 tttcgcctaa aaacagagat gctttaataa aaagatcgtg gaaagaataa tcggaaaaat
     2941 gtataacggg aattgttaac aacaacggac ataaaacgct tgcaataaac tgtatgcccg
     3001 accatatcca tatttttatt gggtacaatg taaaccagtt aatacccaac ttggtggaga
     3061 acattaaaac atcgagcaat gcttgggaaa aaaaggaaga aaaactatca aaatacaaat
     3121 ttgaatggca aagaggatat gaggcattct cccattcgcg ttcacaactc gatactgttg
     3181 tgaaatacat ccaaaaccaa gagcagcatc aaaagaaatc gtttaggaat gaatatctgg
     3241 agattttgcg aaagattgat atcaagtatc agtacgagta cctgtttgaa ttctttgaaa
     3301 atggaggggt atgggattaa aggatagttc aatttgcctt tttatggttt cgctgttcgg
     3361 cagctatacg aggctcttta caccttaatc tttgttgggc gcataccatt aggttcgatt
     3421 cattaaaatc gacttgattg ctatttttgc ccgactttgc ttaaaatgaa ttttttagtg
     3481 taactttgtg ataaattaga atgttatata ttactaatat ttactgatat ttttttaaaa
     3541 ttctgtcgat ggattacaat acccaacgaa aaaagttgcg attaccggaa tacggaaggc
     3601 atatccagca aatggtcgat cacatagcca gcattgatga tagggaggag cgtaacaggc
     3661 ttgccaaatc gcttattgcc attatgggca accttaatcc ccagctaaga gcggttaacg
     3721 atttccgcca taagctttgg gaccacctct ttatcatgtc tgacttcagg ctcgacatcg
     3781 aatcgcctta ccctaagccc gatcttgaaa agtacaatga gaaaccgcgt agggtaccat
     3841 acccaagcca tcccattaat ttcaagcact atggacgtat cattgaactg atgatcgaaa
     3901 aagctattga gatggatgag ggccctgaaa aggaagcctt taagcaactt attgccaacc
     3961 agatgaaaaa ggccaacatc acatggaata aggaatcggt taccgacgat gatatatttc
     4021 gcgatatgga gcatctaagc aaaggcaggc tggttatggc tcccggctca aagctgattg
     4081 ataagcgcga ggttaaacct actggtggta agcggcccaa gcatcatcgt aagcgttaaa
     4141 aaaaactttc tacatggcaa catttgaggt atacggtggt aagcccttaa agggtgagtt
     4201 gcacccacag ggggcaaaga atgaggcact gcaaatcatt tgcgccgcac tactcacagc
     4261 tgaacctgtt acaatcaaaa atatcccaaa catccgcgat gttaataaac ttattgagct
     4321 gcttcagggt atgggtgttg aggttcaaag gcacagctcc tcggagtgca cctttacagc
     4381 caaaaatgtt gatgtagagt atcttaccac taacgatttt aaggttaagg ctgccgccat
     4441 acgcggctct atcatggtgg ttggtccact gctggcgcga tatggcagag catacgcccc
     4501 taagcccggt ggcgataaaa ttggtcgccg acggttggat acacattttc ttggatttca
     4561 gaagttgggc gcaaccttta actttgatac ccgtgagaat ttctttaccc ttgagggtaa
     4621 aaacatgcgg ggtacttaca tgctgctcga tgaggcttcg gttaccggaa cggctaacat
     4681 cctcatggcc gctgtgctta ctccgggtaa aaccaccatt tacaatgctg catgcgaacc
     4741 atacctacag caacttagca agatgctggt tagcatgggg gcaaaaatat cgggtattgg
     4801 ttcaaacctg cttaccgttg agggggttga atcgttgggc agctgcaccc ataccattct
     4861 gcccgatatg attgagattg gttcgttcat tggtcttgcg gccttgacag ccagcgatat
     4921 caccattcgt aatgtatcgt atcctaacct cggcattatt cccgattcgt tccgccgcat
     4981 gggcattgag tttgaggtta taggcgatga tatccatatc ccaccccagg aacactacga
     5041 gattgatacc tttatcgatg gttccatact caccattgcc gatgccccct ggcccgggct
     5101 aacgcccgac ttgctcagcg ttttcctggt ggttgccacc caggcaaagg gctccgtact
     5161 aatccaccag aagatgttcg agagccgact attctttgtg gacaagctaa ttgacatggg
     5221 tgctcaaata atcctttgcg acccgcatcg cgccactgtt atagggcatg accggaagat
     5281 gaagttacgc cccaccgtta tgacatcacc cgatatcagg gcaggcgttg ccctgcttat
     5341 tgctgccctt tcggccgagg gaaagagcgt tattcacaat attgagcaga ttgaccgcgg
     5401 gtacgagaac attgatggcc gacttaatgc cattggtgcc aatattcgca ggactgatta
     5461 ggtgcttttc ctgtaaaatc aaactaactt atcacataaa tttgcacctt taacgtttta
     5521 ataatcaaca gcgcatagtt gcctacaaat gctaatgaga ttttggagta aaatatcaat
     5581 tttaattata tttcttgctg gcgtgggttt tacatcacgg gctcaactta acgccccggg
     5641 aagagttata accctgctta cccagtatag caatacagtt aaccaggatt caatatttgt
     5701 attttatggc gatattggca aacttagtgc ccgccatact acagggaata ttgccaactt
     5761 taagtggtat aggtataacc ctttagtatc aaaccctacc ttacggtttg aactgtttgc
     5821 tgaggaagtt agcgttgtgc aaagcgaaca gttgaaccta aatgagggcg gctacagagt
     5881 ggtaattacc gatattgccg attctaccga aacattcacc tgctggcttt ttactgataa
     5941 tgttagcctc gaccggattg acatcttcaa cagttgccag tttcttgagc ttaattccat
     6001 cacaataccc tcatcgtaca acgttgccta cgaccgtttt acctaccacg acctttcgcg
     6061 ctcaaatcaa ccagaacgga atacctacgg tcagcagtac tttgcaaatg ttacctggca
     6121 ggcctcggag agcagtattg agctgccatc gtcctcagca ttaaagttgg ttattgagaa
     6181 tcctgcacct ctgcataatt ccacttatac cgttaccatc caaaaccctt ttgggagaac
     6241 ccttacccta acaacgcctg aggtaactgc cgttgctact aaggccgata atctaatcca
     6301 ggttgacgaa aggggagtgt ggaacgattt tgatgcgaat gccgagtatg aggcgctgct
     6361 gggcctaagg ctcgaaagca aaagtctgaa ttgcgattcc atttactggc gtattacaac
     6421 ccgaaagatt tctcccgaaa gcattttcga tatttctgtt tggcgcgata gctctttaat
     6481 tgcacagcgc aacgaggcct tccccgataa aaaggtaatg gttcccggat tctacaaggt
     6541 ttaccattac tcggtaaata ccctctcggg gtgtatcgat tcggttatta ctgatattaa
     6601 ggttgactcc tcgcgcataa gcgccgatgc catccccaat gttttttcac ccaatggcga
     6661 tggaaagaat gatgtgttcc gcttttccga taccgatgag agcattcgct caattcgttc
     6721 gtttaccata cgtatttata gccgtagcgg caagctggtt tacacataca gtggtaatcc
     6781 aagcgagtgg aaaggatggg atggtcgaac cagttcagga tcagatgctg ccgagggtgt
     6841 ttattactac attatcgaag cccgaggctg ggacgaccgc cgttttgccc gtggccccta
     6901 caaggggttc ctgcatctgt ttagggggag gtaggggcag agtattgtgt ttggttgttt
     6961 ttgattatga atagtttaga acgctggctg cgtataacac aaaacatcaa aacccctttg
     7021 tccgagaacg tcttccagct ctgttcgctc cttcgcattt ttaggggtta ataagatcct
     7081 gcgctacaat tcgatatgcc cagtacaggc tactcaggat ggcaggtgga gtttgttagt
     7141 gtattgttgt tcgctgaaaa tgaatatatt atagtcctaa ttacgcagaa cactatattc
     7201 actaaacact aaactctata cacgctcccc ttattcctca acaatcacaa tcttttcaat
     7261 cctatcgcca acacggatag catcaattac ttcaaggcct tcgtaaacct taccaaagca
     7321 ggtatgctgg cggtcgaggt gcttggtgtt ttgcctgttg tggcaaataa agaactgtga
     7381 accacccgtg tttcggcctg catgcgccat ggaaagtacg ccacggtcgt ggtactggtt
     7441 atttccggta agttcgcagt taattttata gccgggtcca ccggtaccca cccggggatc
     7501 ggtcatatcc ttggagaagg ggcatccgcc ctgaatcaca aaattgggga ttacacggtg
     7561 aaaggctaac ccatcgtaaa agccttcatt ggccaactta acaaagttag caacggtgtt
     7621 tggggcatcc tcctcgtaga acttaacctt cattacccct ttcgatgtgt gaatttctgc
     7681 tgttctcatg gtattgtttg tttggcacaa aggtataaaa aaaagagggc taaccccaat
     7741 tggtagtcct cttttgctga acagggtatg gctattcttc ctcttcctgt tcctggtatg
     7801 cctttaggag cttttcctga acatcggcag gcacctgctc gtatgaggca aacttcatgg
     7861 tgtaggtagc tctaccgctg gttaacgatg atagggcagt ggagtagcgg tttagttcag
     7921 ctaggggaac cttggcatta atcacctcaa atcccttaac gctgcccata cccatgataa
     7981 tggcgcggcg gttctgcagg tcgctcatca catcgcccat acggtcggca ggaactagca
     8041 cctcaacatc gtaaatgggc tccatgatct taggcccggc atccttgaat gcgttcttga
     8101 atgcgttgcg gccagcaagc ttaaacgaga tttcgttgga gtcaaccggg tgcatcttac
     8161 cgtcgtaaac ggcaacgcgg atatcgcggg catatgaacc ggtaagcggg ccctgctcca
     8221 tcttctccat aatgcccttg aggatggcag gcataaagcg agcatcaatt gctccaccta
     8281 caatgcagtt atagaacacc aacttgccgc cccattccag gtcaataact tccttccccc
     8341 taaccgagag gttgatttcc ttaccgttaa ccttgtactt aacgggatcg ggtgcaccct
     8401 caacgtaggg ctcaataacc aggtgaacct cgccaaactg accggctcca cccgattgtt
     8461 tcttatggcg atagtcggcc tgggcaacct tggtaatggt ttcgcggtat gggatacgtg
     8521 gggctatgta ttcaatctcg agcttgtgta ggttggtaag ctgccacttc agaatattga
     8581 ggtggaactc accctgaccg gaaaggataa tctggcggag ctccttggag tgttcaacca
     8641 gaatggttgg gtcttcctgg tgggcacggt taagcagttc gccaagcttt tcaacctcgg
     8701 cctcgttttt aggcttaaca gcggtgcgga acttgggatc ggggaattcc atgggatcga
     8761 aggtgaaatc cagacctggt ccgcaaaggg tatggttggt cttactgttc ttgagcttta
     8821 cggtagcgcc tatatcgcca gcaaccagct cggtaacctt gttacggttc ttgccggcta
     8881 caacaaatag ctgcgaaagg cgttccttgg ttgagttgtt ggcgttggtt aggtccatac
     8941 cctcagttac cttaccggac attacgcgga agtagctgat ttcgccaatg tgtggctcaa
     9001 cggtcgattt gaaaatgaaa agcgaggttg gagcgctggc gtcgcaggta atgggtttat
     9061 tgttattgtt tttaagaacc ggtgagtggg taggattagg cccaacgtta atgataaaat
     9121 ccatgaggcg cttggtgcca atgtttttag ctgccgatag gcagaaaacc ggcataatgc
     9181 tgcggctggc aatacccttt gaaaggcctg agcgcatctc gtcctcggag agcgaaccct
     9241 gctcaaagaa cttctccatg agcgattcgt cgttctcggc agcagcctca atgagcgcat
     9301 tgtgaagctc agtggcctta tccatttcgc tgtcgggaat ggggaactcc tcgcggatac
     9361 cggtatcacc cttaaactta tacattttca tggtgattac atcgataaag ctatcgaaag
     9421 cagcaccggt ggcaacgggg tactgggtta agataacctt attaccgaag gtttgcttca
     9481 gcgagtcgag cgtggcatca aagttagcct tatcgtggtc gagctggtta acggccagta
     9541 tcatgggctt gtgaaggtgc tcggcgtgac ggttgaagat ttcggtacca acctcaaccc
     9601 cgtactgggc attaataacc attaccagca aatcggttgg gtacatcgat gagataacag
     9661 caccaacaaa gtcgtcggat cccggagtgt caataatgtt tagcttgctg ttattgaact
     9721 cggtgtatag caaggttgaa aaaattgacc tttggttctg ttgttcaatt tcggtgtaat
     9781 cggaaacggt gtttttccca tcaattgttc ccttgcgttc aattatttta ccttcaaaca
     9841 tcatcgactc ggccagtgaa gttttgcccg agccggtatt acccagtaag gcgatattcc
     9901 taatttgttc ggtttggtag gttttcatgc ggtttacgct tatttttggt ttaacataag
     9961 gttattttat tttttccaat cgcgggtatt catatatagt aaaaggtata tccctttaca
    10021 accaattccc ttgactggtt accctttttt tgggtagccc aaaaatagta aaattttgtt
    10081 tccaacaaat aaaatttgcc aaaagcctta tgttgtaaaa cattttgggg cacagggccg
    10141 atatttttta caagttacta agaatcagga ttgttttttt aaatcaaaag ttataccttt
    10201 gcgcccgcaa aaagcaaacc ggctaaaaaa gctgatgtac aaaaaagttg atttgcatag
    10261 ttgtaattgt aaaaaaatca atacctttgc agtccatttt ttaaacttaa actataacaa
    10321 taaattggat ttatgccaac aattcagcaa ctagtaagaa aaggaaggtc taaactggtg
    10381 gataagagca aggctccagc ccttgatgct tgcccccaga gacgaggtgt atgtgtacgc
    10441 gtgtacacca ctacgcctaa gaagccgaat tcggctatgc gtaaggttgc cagggttagg
    10501 cttactaacg gtaaggaggt taacgcctac attcctggtg aaggtcacaa cttgcaggag
    10561 cactccatag tgctaatccg cgggggtagg gtaaaggatt tgcccggtgt gcgttatcac
    10621 ctcattcgcg gaacactcga tgcttccgga gttgagggtc gcaaacaggg ccgttccaag
    10681 tacggcgcca agaggcctaa ggataaggct agtaaaaagt aaaagttaac ctcatttgaa
    10741 ttggttgaac aatgagaaaa gcaaaaccaa aaaaacggat tatcttaccc gatcccaagt
    10801 ttggcgatat aatggttacc aagtttgtga ataacttaat ggtaacgggt aagaagagta
    10861 ttgccttcgg aatcttttac aatgcacttg atatagtagg taaaagaatg aaggacagcg
    10921 aaaaatcgcc ccttgaaatc tggaagattg cccttgaaaa cgttacccca caggttgagg
    10981 taaagagccg cagggtgggt ggtgctactt tccaggtgcc caccgaggtt cggcctgagc
    11041 gtaagcaatc gctatccatg aaaaacctta ttctttatgc ccgcaaacgc gttggtaagg
    11101 gtatgagcga caagcttgct gcagagatta tggctgctta caaccaggaa ggtggtgctt
    11161 tcaagcgtaa ggaggatatg cataagatgg ctgaagctaa caaggctttt gcacatttcc
    11221 gtttttaaga cccattagta cgaggaaggt aggagattga tgagcagaga tttaagattc
    11281 actcggaata ttgggattat ggcccacatc gacgccggta aaacaacaac taccgagcgt
    11341 atcctgtttt atacaggtaa aactcacagg attggtgagg tacatgatgg ggctgcaacg
    11401 atggactgga tggtgcagga gcaggagcgt ggtataacta ttacctcggc cgctactaca
    11461 actttttgga actacgagaa caagcagtat caaataaaca ttatcgacac cccgggacac
    11521 gtggacttca ccgttgaggt ggagcgatcg ctccgcgtac tcgatggcgc tattgccact
    11581 ttctgtgctg tgggtggtgt tgaaccccaa agcgaaaccg tttggcgtca ggccgataag
    11641 tacagggttc cccgcattgc ctatgttaac aagatggacc gtgtgggtgc cgatttcctt
    11701 ggggtggttc aggaaattaa agacaaactg ggtgcaaatc caattcccat tcaactccct
    11761 atcggggccg aagataactt taacggcatt atcgatcttg ttgagaataa ggccgttgtt
    11821 ttctttgagg acgaaaagct gaaggctacc aactaccgtc tggagcccgt tcctgccaac
    11881 atgaaggacg aggttgacga gtggcgtgcc aagcttattg aggcagttgc atcgtacaac
    11941 gatgagctcc ttgagcgttt ctttgaagac cccgattcca ttaccaagca ggagattatt
    12001 gaaactatcc gtaaggccac aatcgacatg tcgattattc cggttctttg cggatcgtcg
    12061 ttcaagaata agggaataca acacttgctc gatgcggtta ttgccttcct acccagccct
    12121 ctcgataagg gtgatgtggt tggcacaaat cctaacaccg gtaacgaaga ggttcgtaaa
    12181 ccatacatcg atcagccttt ctgcggttta gcattcaaga ttgctaccga cccctttgta
    12241 ggccgtctgg ccttcatcag ggtttactcc ggaaaggttg atagcggtag ctacgttttc
    12301 aacacccgta ccggtaaaaa ggagcgtatt agccgtattt accagatgca tgccaacaaa
    12361 cagaacccaa ttgagttctg cgaggctggc gatatctgtg cagcagttgg ttttaaagat
    12421 attagaacag gcgataccct ttgcttggag gaaaaaccta tagcccttga ggcaatgact
    12481 ttccctgagc ctgtaatcgg tttggctatt gagcccaaga cccaaaagga tcttgacaaa
    12541 ctcggtattg cgcttggtaa actttcagag gaagacccaa cattccgtgt aaacaccgac
    12601 cccgatagcg gtcaaaccgt tattagcggt atgggtgagt tacaccttga gatcattgtt
    12661 gaccgcctac gtcgcgagtt taacgttgag atcaatcaag gtgctcctca ggttgcttac
    12721 cgtgaggcat ttaccaggag cgtttcgcac cgtgaggtgt tcaagaagca aaccggtggt
    12781 aagggtaagt tcgccgatat catttttgaa cttggtccag ccgacgaagg tgttaccgga
    12841 ctgcagttta ttgacgaggt gaagggtggt aatattcccc gtgaatttat cccctcagta
    12901 caaaagggct ttgagttagc catgagcaac ggtatactcg ccggctatgc cataccaagc
    12961 atgaaggtgg tgctcaagga tggttcgttc catccggttg actccgatgc tctctcgttc
    13021 gaaattgctg cccgctacgg attccgtgca gcaggtccaa aagccgctcc ggttttactt
    13081 gagccaatta tgaatgttga ggtggttacc cccgaggaga acatgggaga tgtaataggt
    13141 gacctcaaca agcgtcgtgg ccagattgcc ggaatggaat cgaagggtaa cgcccgcgtg
    13201 gttaaggcta aggttcccct tgccgagatg tttgggtacg taaccgtact ccgtacacta
    13261 acctccggac gcgccacttc aaccatggag ttctcgcact acgccgaagt acccgctaac
    13321 attgctaagg aggtgattga gaaatccaag ggcaaagagc taaaagaaat tgaataatta
    13381 cttttatccg ataagtacaa tgagccaaaa gattagaatt aaactcaaat cgtacgacca
    13441 caacctggtt gacaagtcgg ccgaaaagat cgttaaaacg gtcaaggcta caggcgctgt
    13501 ggtgagcggt ccaattccgc ttccaacaca taagcggatt tttacggtta accgttcaac
    13561 attcgttaac aagaagtcga gggagcaatt tgaactcagc tcctataagc gtttgctcga
    13621 tatctacagc tccaatgcga agactatcga tgctctgatg aagctcgaac ttcccagtgg
    13681 agtcgaagta gagattaaag tatgagtgtg aattgtaaat tagaaagaca atgccacaag
    13741 gattaattgg acgtaaaatc ggaatgactt ccgtattcgg tgaggatggt aagaatatcc
    13801 cctgcaccgt aattgaggct ggtccttgcg ttgtcacaca ggtgaaaaac gctgaaaccg
    13861 atggctatgc cgccattcag cttgcctatg atgacaaaaa ggagaaacac accagcaggc
    13921 ctatgctagg ccatttcaag aaagccaaca ctactcccaa acgtaagctg gttgaattcc
    13981 gcgattggga aggattacag cttggtgatg tagtaaccgt tgacctcttt gccaatgaca
    14041 actgggttga cgtagttggt ataaccaaag ggaaaggatt ccagggtgtt gtaaagcgcc
    14101 acggttttaa cggagtgggc ggaagaaccc acggtcagca caaccgtcag cgtcacccgg
    14161 gttctatggg tgcatcttcg ttcccttcac gcgtactccc cggcaagcgt ttggcaggcc
    14221 gtaccggagg cgacagggtg aagatcatca acctacgtgt tcttaaggtt atccctgaga
    14281 gcaatctttt actcgttaaa ggatcgattc caggggcaaa gggttcttac ataattattg
    14341 agaagtaatg gaaatcaagg tacttaacat acaaggaaag gaaaccggca ggaccattca
    14401 gttggaagac tcgatttttg gtatcgaacc caacgaccat gccatttacc tcgacgtaaa
    14461 gcaaatccta gctaaccgtc gtcagggtac gcacaaatcc aaacagcgta acgaggtttc
    14521 cggtagtacc cgtaaactca aacgccaaaa gggtaccgat actgctcgcg caggtagcat
    14581 caagtcgcct gtgttccgtg gtggtggacg tatatttggt cccgttccac gcgactatag
    14641 ctttaagcta aacaagaaga ttaagcagct ggctcgtaag agcgctcttg catacaaggt
    14701 taaggagaat gctctacttg ttattgaaga tttcagcttt gatactccaa aaaccaagga
    14761 atttgtaaat atctgcaaga acctgaacgt agccgacaaa aaaacactct tcttgttggg
    14821 cgagacaaat aaaaatatat atttgtcgtc cagaaattta cagaagcaag caactgcaat
    14881 tgcctcatca gtaaacactt acgagatttt aaatgccaac atgttggtgt taactgagag
    14941 ttctgtagat gctctgaaca aaatgtttaa ggtatcataa caaggaagcg ccatggatat
    15001 actgataaaa ccagttgtaa ctgaaaagat ggagcgctta accgataagc tgaaccaata
    15061 cggctttatc gtggataaga gagctaataa gttacagata aagaaggcaa tagaggagtt
    15121 atacggggta accgttgact cagtgaatac catgcgttat gccggtaaag ttaagtcccg
    15181 ttacacccgt actggtgtgt tggtgggccg taccaatagc tacaaaaaag ctatcgtaac
    15241 gcttaaggaa ggagataaga ttgattttta tagcaacatt taactaagat ggctgtacgg
    15301 aaactaaaac ctgttacacc aggacagaga cacaagatta taggtttgtt cgatgacatt
    15361 accacaaaca aacctgagaa gtcgttacta gtccccctga aaaagactgg tggtcgcaac
    15421 aaccaaggcc gaaggaccat gcgatatata gggggaggcc acaagcgcag atacagatta
    15481 atcgatttcc tgcgcaaccg tgagaatgag ccggctgtgg taaagactat tgagtacgat
    15541 cccaaccgca ctgcacgtat agcgctggtt gagtaccccg atggcgagaa gcgctacatt
    15601 gtagcccctg cagggcttaa ggtaggccaa accatcatgt cgggtcgcgg tgttgctcct
    15661 gaggtgggta atacccttta cctgggcgaa atacccctag gtaccattat ccacaacatc
    15721 gagctgtacc ctggccgtgg tgcttgcatg gcacgtagcg ctggatcgta tgctcagcta
    15781 ctagcccgtg aggggaaata cgctattatt aagctgccta gcggcgaaac ccgcatggta
    15841 cttaacgagt gtaaagcaac cattggtagc gtgtcgaact ctgaccatgc attggaaaaa
    15901 tccggtaaag ccggacgtag ccgttggtta ggtcgtcgtc ctcgcacccg tggtgttgcc
    15961 atgaaccctg ttgatcaccc catgggtggt ggtgaaggac gtgcctcagg aggtcaccca
    16021 cgctcgcgca agggtattcc tgctaagggt tataagactc gttccaagaa gaaacacagc
    16081 agcaagttca tcattgaacg caggaaaaag taatttgtaa aaaactgtaa ctatgagtag
    16141 gtcacttaaa aaaggtccct ttattgacta caagttggag aagcgtgtca ttcagatgaa
    16201 cgaatccaac aagaagtctg ttatcaagac ttggtcaagg gcttccatga tatctcccga
    16261 ttttgttgga cacaccatag cagttcacaa cgggaataag ttcattcctg tgtacattac
    16321 cgaaaacatg gtaggtcata agctgggcga gtttgctccc acccgcacct tccgtggcca
    16381 tgctggtaat aagaacaaat agtgaattga taaaaggaaa aagagatggg tgcaagaaaa
    16441 cataacatgg ccgagaggct taaagcggag aggaaaaaca aagcagttgc ccgcttacgc
    16501 aactgcccgt cttcgccccg taagatgcgc ttagtggttg acctggttcg cggtaaagac
    16561 gtaaacacag cgctcaacat acttaaattc agccgcaagg atgccgcaaa ggatgtgcac
    16621 aagctaatcc tttcagcagt tgctaactgg aaggctaaaa acgagaacgt acgcattgaa
    16681 gatgccaacc tttacatcaa ggagatcttt gtagatcagg gcagaatgct aaaacgtatt
    16741 cgtacagcac cacagggacg cgctcaccgc atccgtaaac gctcaaacca cgtaaccgtt
    16801 gtggttgata gcgcagttaa taacgaatca aacaactaac agctacatgg gacagaaagt
    16861 taatccaata gcaaaccgtt taggaatcat ccgtggatgg gattccaact ggtacggagg
    16921 taaaaaatac gctcagaaat tggttgagga tagcaaaatc cgcgactacc tgaatgccag
    16981 gcttgccaag gccagcctct caaaaatagt tattgagcgt accctgaagc tgataaccgt
    17041 aaccatacat actgctcgcc caggtatcat tattggtaag ggcggacagg aagtggataa
    17101 gctacgcgag gagctacgta agattaccaa caaggatgtt caaatcaata tctttgaggt
    17161 gaagcgcccc gaaattgatg ctgttatcgt aggtaacaac attgctcgcc aaattgaagg
    17221 acgcatttct taccgtaggg ctattaaaac agccattgcc tcaaccatga ggatgggtgc
    17281 tgagggtatc aaggtgcaaa tttccggccg cttgggtggc gctgaaatgg cacgctctga
    17341 aacctacaag gagggacgaa ttcccctaca caccttccgt gccgatatcg attacgctct
    17401 agccgaggct cacaccaagg ttggcgttat aggtattaag acctggattt gcaacggtat
    17461 agtttacggc aagcgcgatc tttcgcccaa cattggtaca aagagcgttg ccaacgtaac
    17521 cgagaaaaag gcaaaaccag cacctaaggg aggttcacgt cctcgtgcca agaaaaagaa
    17581 atagtaaggt gtataaacac taaagcatta gcgaaatgtt acagccaaag aaaacaaaat
    17641 acagaaggca gcaaaaggga aaaatgaaag ggattgccca acgcggaaac cagttggcat
    17701 tcggttcatt tggaattaaa gcccttgagt cgagctggat cacaagccgt cagattgagg
    17761 ctgcccgcca ggccgtgacc cgttacatga agcgtgaagg tcagatctgg atcagaattt
    17821 tccccgataa gcccattacc aagaaacctg ctgaggttcg tatgggtaaa ggtaaaggtg
    17881 ctcctgaggg gtttgttgct ccggttactc caggccgcat tctgtttgaa tgcgacggcg
    17941 taccctacga agttgcaaag gaagcattgc gcctggcagc tcagaagctc cctattacta
    18001 ctaagtttgt tgtccgtccg gactttgttg aacctacaaa ctaattgagc catgaaagta
    18061 gcagaaataa gagagctcac cgataaggag attcttgagc gtattgagct ggaaaaaacc
    18121 aacctgctta agctaaggtt gaaccatagc atatctcctt tggataatcc aatgaaaatt
    18181 aaagagaccc gcaggaacat cgccaggtta caaaccgtcc tgaaccagcg gaagctcagt
    18241 caaaacaagt aatgcgttgt agctatggaa caagtaaacg aagtaaagag aaataaccgt
    18301 aaggagagta ttggcgtggt ggtgagcaac aagatgcaaa agtcaattgt tgttgccgta
    18361 aagcgtaaag taaaacaccc catttacgga aagtttgtga ataaaaccac caagttttac
    18421 gctcacgacg agaataatgt atgcgaggtt ggcgatttgg ttcgcatcat ggaaactcgc
    18481 cctctcagca agcttaaacg ctggaggtta gttgaaatca tcgaaaaggc taagtagtca
    18541 tgatacagca ggaaagtaga ctagtagtag ctgataatag cggagccaag gaagtgctct
    18601 gcatcagggt actgggaggt actggtaaac gctacgcccg tataggcgat aagatagtgg
    18661 ttaccgttaa gagtgccatt cccggtggtg atattaagaa aggtaccgtt tcaaaggccg
    18721 ttgtggttcg taccaagaag gagatccgcc gtcaggatgg ttcatacatc cgtttcgacg
    18781 ataacgcttg cgtattgctt aacaaccagg gcgaaattcg tggaactcgt atctttggcc
    18841 cggttgccag ggaactacgt gataattaca tgaagattgt ttcacttgca cctgaggtgt
    18901 tataaacccc aataactgta gaattatggc aaagttacac atcaagaaag gggatatggt
    18961 ttacgtaatc tccggtgaat ctaaaggcca ccagggtcgc gtactcagtg tgctcgttaa
    19021 ggagaaccgt gcaattgttg agggtgctaa catggtatcg aggcatacca aacccaattc
    19081 caagaaccct cagggtggaa ttatcaaaaa ggaagcccca attcatattt caaacctaat
    19141 gctggttgac cctgccactg gcaagccaac ccgcatcggt cgtcggttga acgaccaggg
    19201 taaacttgtt cgttactcta aaaaatcagg agaggagatt aagtaatgga atacgtacca
    19261 accctaaaga aaaagtatag ggaggagatt gtccccgcgc tcatgaaaca gttcaactac
    19321 aagagcgtga tgcaggttcc ccggttggaa aaaatagtga tcaaccaagg gataggacaa
    19381 gcagtagccg atcgcaaact cattgagcag gcacaggcag aactcaccat gattgccggt
    19441 caaaaggcaa ttcaaaccta ctcgcgtaag gatatatcaa actttaagct ccgtaagggg
    19501 gttcccattg ggcttaaggt tactctccgc cgcgaaagaa tgtatgagtt ccttgagcgt
    19561 ttaatcaacg tttccttgcc gcgtatccgc gactttaagg gaattaacga caagttcgac
    19621 ggccgtggta attacaccct tggcattcag gaacagatta ttttccctga aattgacatc
    19681 gataaggtga gcaagattct gggtgtcgag atcactttcg taaccacatc ggagaacgac
    19741 gaggaggctt atgcacttct tcgtgagttt gggttacctt ttaaaaatgc taaaaaggaa
    19801 tagactatgg caaaagagtc aatgaaagcg cgtgaggttc gccgtgcgaa actcgtagca
    19861 aaatacgccg aaaagagagc caggctcaaa gaggaaggcg attacgttgg gcttcagaag
    19921 ttacctcgta actctaaccc catccgtttg cgtaatcgtt gcatgttaac cggaaggccc
    19981 agagggtata tgcgccagtt tggcatcagc cgtatcacct tccgcgaaat ggcctcaaaa
    20041 gggttaattc ctggtgttaa gaaagctagt tggtagaatt taataataaa acgaagcaat
    20101 gattaccgac ccaattgcag attacctaac ccgagttcgt aacgccgtga tggctcgtca
    20161 ccgggttgtt gaggtacccg cttcgaatat gaagaaggat atcaccaaga ttcttttcga
    20221 taaggggtac atactaaact ataaatttga ggatgatggc aagcagggca ttatcaaaat
    20281 cgcacttaag tatcatcctg agactaaggc atgtgccatc aagcacctgg aacgcgttag
    20341 tactcccggc ttgcgtcgct acagcaacgc aaaagatctt ccccgcgtgt taaatggcct
    20401 gggtattgcc attatcagca catcgcaagg gttgatgacc gacaaggagg ctcgcgccaa
    20461 gaatattggt ggtgaagtgg tttgctatgt ttactaatcc agtataattt ttaaactaat
    20521 gtcgagaata ggaaaaaaac ctgtaaactt accacaagga gtaagcgtta acattagcgc
    20581 cgataacgtg gtaagcgtga aaggccctct cggagaacta aagcaaaaag ttgatccgga
    20641 tatcaccgta aaggtagagg gaaatgaagt tgttctctcc agaccaactg atcataagcg
    20701 tcaccgttcg ctacatgggc tataccgtgc ccttatcaac aacatggtaa ttggtgtatc
    20761 caagggcttt gaggtacagc aggaacttgt gggtgttggt tacaaggccg aagctaaagg
    20821 tcaaatcctt gagatgagcc ttggtttctc ccacgacatc tactttgaga tgcccaagga
    20881 aattaaggtt gaaaccatta ccgagaaaaa gggtaacccc aagatcattc tgaagagcat
    20941 cgataagcag ctcttaggtc aggttgccgc taagatccgc tcgcttcgta agcctgagcc
    21001 atataagggt aaaggtattc gctttgtggg tgaagttgta cgtaagaaag ctggtaagtc
    21061 ggctagtgta taacccgtaa aactgaaaga gagatatggc tttaaataaa ttggaaagaa
    21121 agctgcgcat tcgcagacga atccgcaaaa gagtttcggg aacagctcaa aaacctcgta
    21181 tgtcggtttt caggagcaac accaacatat acgttcagtt cattgacgat atcaatggtg
    21241 taacactagc atcggcctct tcgatgtcaa aagagattgc agagaaaaaa aatattacta
    21301 aaaccgaaca agcccgactt gttggacagc ttgccgctaa aaacgccatg gctaagggta
    21361 ttgagagtgt ggtgtttgac cgtggtggta acctatacca tggccgcgtt aaggctttag
    21421 ctgatgccgc aagagaaggt ggacttaaat tctaagcacg atgtcacaga ataccaacat
    21481 acgaagagta aaagccagcg accttgagct caaggacagg ctggttagca ttcagcgcgt
    21541 aaccaaggta accaagggtg gtagaacttt cagcttctca gccattgttg tagtaggtaa
    21601 tgagaatggc gttgtagggt atggcttagg taaagctagc gaagtaaccg ctgccattgc
    21661 caagggtatt gaggatgcta aaaagaatct ggttaaggta cccatactaa agggtactat
    21721 cccccacgaa caggaagcca agtttggtgg tgccaaggtg ctgattaaac ctgcttcgca
    21781 cggtaccggt gttaaggctg gtggtgctat gcgcgccgtg cttgagagcg tgggtgtaac
    21841 cgacgtactt gcaaaatcaa aaggttcatc aaaccctcac aacctggtta aggctaccgt
    21901 tatggcattg cttgaactgc gcgatgctca caccgtggcc cagcagcgtg gggttgacct
    21961 taaaaaagta ttcaacggat aaaaactgat gactatggca aagattaaag taacacagat
    22021 acgcagcgtt attggccgcc ccgaagggca acgcttaaca cttcgttcgc tgggaatccg
    22081 ccgtatgcat cataccgttg aggtagaggc taccccacag gttatgggta tgattgctaa
    22141 ggtgcgtcac cttgtgcgaa ttgaagagca ataggtataa caactaacga ttgtaaggtc
    22201 atgaaactag ataaactaaa acccgctgaa ggttctaccc acaacaggaa acgtattgcc
    22261 cgtggtgagg ggtcaggaca tggaggcact gccactcgtg gtcacaaggg tgccaagtca
    22321 cgctcaggtt actcccgcaa gtttggcttt gagggtggcc agatgcctat acagcgtcgt
    22381 ctaccaaagt ttggctttaa aaacccattc cgcgttgagt acaagcccat taacctggaa
    22441 gtgttgcaag cccttgccga aaagcacggc tataccacta tcgatattga tacccttatc
    22501 aatgccggtt tagtgtcgcg caacgatatg gttaaaattc ttgccaaggg tactcttagc
    22561 gctaagcttg aggtgaaagc ccacgctttc tcaaagaaag caattgaagc aattgaagca
    22621 gctaacggaa ccgcagtaaa actatagtga ataatgaaag cctttttcga aaccctcaag
    22681 aatatataca agatagagga tctgcgcaag cggattctct atactcttgg tattctgctg
    22741 atttatcgac ttggtagctt tatagtaata ccgggtcttg acccatcgca gcttggcgct
    22801 ctgcaagagc aaaccaagga aggtatcatg ggcctcctcg acatgttttc gggtggtgca
    22861 ttccataatg cctccatttt tgccttgggt attatgccct acatctcagc atctatcgtg
    22921 gttcagcttt taggaattgc tgtaccctac ttccagaggt tacagaaaga aggcgagagt
    22981 ggtagaaata agcttaacca gattactcga gtacttacag taggtatcct gctattccag
    23041 ggtccagcct acataaccaa cctttacatt cagcttccac agggagcatt cctgttaagt
    23101 aagttctggt tcaccattac agccactatc accatgattg gtggtactat gttcatcatg
    23161 tggttgggtg agaagattac cgataagggt ataggtaacg gtatatcgct aatcatcatg
    23221 attggtatta ttgcccgctt accctttgcc tttgttgcag aacttttctc aaaggccgaa
    23281 gagcttggtg gtggattggt tatgctccta attgagcttg taatactatt tgcagtattc
    23341 atgttctcaa tcctgcttat tcagggtaca cgtaaaatac ctgtgcagta tgccaaacga
    23401 attgtaggaa ataaacagta tggtggtgtt cgccagtaca taccccttaa ggtaaacgct
    23461 gccggtgtaa tgccaatcat ttttgctcag gctctgatgt ttatccccat gctgtttgcc
    23521 cgctttgatg ctactgctgg cattgctgcc gcttttagct acaccggatt cctatacaac
    23581 ttcacctttg gattgctgat catagtattc acctatttct ataccgctat tattatcaat
    23641 cccaaccaga tggctgagga tatgaagaag aacggaggtt ttattcccgg agtaaaaccc
    23701 ggaaagaaaa ccgttgagta tctcgactcc atcatgtcga aaatcactct acccggttca
    23761 atcttcttag ctattatagc tattcttcct gcatttgcca ttaagctggg agttaacaac
    23821 cagtttgccc agttctttgg cggaacctcg ttgttaatct tagtaggtgt agttcttgat
    23881 acactacagc agattgagag tcacctgctg atgcgtcatt acgatggatt aatgaaatcg
    23941 ggaaggctga agggccgttc cggtatgtaa tagggcaatt ttgccaaacg ttctttataa
    24001 tgtttttagc taaaaccgac gaggaagttg agctgctaag gctaaacaac gatctggtat
    24061 cgcgcacatt agccgaggtg gcaaaacata ttaagccagg ggtaaccacc caaaagctcg
    24121 actcaatagc cgaggagttc atcagggctc atggggctgt tccgggtttt ttggggtata
    24181 atggctaccc agcaacgctt tgcatttcgg tcaacgatgc agtggtgcat ggcattccat
    24241 cgtcgtatga gctccgcgag ggcgacatag tatcggtcga ttgcggaacc ttcttgcacg
    24301 ggttttatgg cgattccgcc ttcacctttc cggtgggcga ggttaagccc gaagtgcagc
    24361 ggctgttaaa ggttacccgc gaatgccttg agctaggcat tgaggctgca gtagccggtg
    24421 gcagggttgg cgatatatcc tacgccgtgc aatcgcatgc cgaatcgaac ggttactctg
    24481 tagtccgcga attggtggga catggcatag gccgccatat gcatgaaaaa cccgaagtcc
    24541 ctaactacgg agcacgaggc cgtgggccta agcttgtcga tggaatggca atctgcattg
    24601 agccaatgat taaccttggc tcaaagcacg tttaccagga tctggatgga tggaccatcc
    24661 gtacccgcga cggtaaaccc tcggcgcact ttgagttggc cgttgttgtt cgaaaggata
    24721 aagccgaaag gctttcaact ttcaagtata ttgatgaagt tttacgttta agttaaattt
    24781 ttaagagcga atgtccaagc aaactgcaat tgagaaagac ggaactatta ttgaggccct
    24841 atcgaacgcc atgttcaggg tagagctcga taacggacat gttattaccg cccacatctc
    24901 cggtaaaatg cgtatgcatt acatcaaaat tttacccggc gataaggtga gggttgaaat
    24961 gtcgccttac gatttaacta aaggacgtat tacttttagg tacaagtaaa ccaaaaaatt
    25021 tcaatacaat gaaagtaaga gcatcagtaa agaagcgcag cgacgagtgc aagatagttc
    25081 gtcgtaaggg acgcttgtat gtgattaaca aaaaaaatcc taagttcaag caacgtcaag
    25141 gataatttgt ttaattttgc acactaatta ttaagtgaaa atatggcacg tatagtaggt
    25201 gtagatttgc cgaaaaacaa gagaggcgag gtagccctta cctatattta tggtattggc
    25261 cgcagcagcg cccgtaagat tctggacaaa gccggaataa gctacgatac caaagtagcc
    25321 gattggaccg atgataatgt aacagccatc cgtaacatcc tgaacgaagg gtacaaggtt
    25381 gaaggtgaac tgcgttccat ggttcagctc agcattaagc gcttgatgga tattggttgc
    25441 tacagaggta ttcgtcatcg tcttggttta cctgtgagag gtcaatcaac caagaataac
    25501 gctcgtacac gtaaaggaaa gagaaaaact gttgccaaca agaagaaggc tactaaatag
    25561 taattaagct atggcaaaga aaacagcaac taagaaaaaa gtagttaagg ttgatccggt
    25621 aggacaagcc catgttcatt catcgtttaa caatatcatt gttacaatga ccaacagcac
    25681 cggtcaggtt atttcctggt cgtcggctgg taagatgggt ttcaagggtt caaagaagaa
    25741 tactccgtat gcaggtcaaa ctgcagcagc cgattgtgct aaagtagcct acgatatggg
    25801 cttaaggaag gtaaaagtgt ttgtaaaagg acccggcgcc ggccgtgaat cggctatccg
    25861 taccatcaac tctaccggta ttgaggtaac tgagattgtt gatgttactc cactaccaca
    25921 caatggctgc cgccctccta aacgtcgtag ggtataacca ttttagtacg aatcaataaa
    25981 attacaaaat aatggcaaga tatactggac caacaaccaa gattgcccgt aaattcggag
    26041 agcccatcta tgggccggat aagtacttcg agaagaagaa ctatcctccc ggggttcacg
    26101 gcttaatgaa gaaacgcaaa aaggtttcgg agtacggaac ccagctgctc gagaaacaaa
    26161 aagctaagta cacttatggc ctacttgagc gtcagtttgc taacctattt aagcaagctg
    26221 ctcgcagcaa gggtatcacc ggtgaaattt tgcttcagct gctcgagtcg cgtctcgata
    26281 acgttgtata ccgtttgggt attgctcctt cacgtgcagc tgcccgtcag cttgtgttgc
    26341 ataagcacat tgttgtaaac ggtaaggttg taaacgtacc ctcattcagg ttacgtcccg
    26401 gtgatgttgt tggcgttcgt gaacgctcaa aaacactcga ggctattacc gattcccttc
    26461 agtccaaccg tcacatcaag tactcatggc tggagtggaa taacgataaa atggaaggca
    26521 aattcatgaa cgtacctgag cgttcggaaa tacctgagaa tatcaaggag caattaattg
    26581 ttgaacttta ctctaagtaa taaaacctgt accatatggc aatactggca ttccaaaaac
    26641 ccgataaggt tatcatgctt gaatctaccg ataacttcgg taagttcgag tttcgtcctc
    26701 ttgagccagg ctatggtatc acggtgggaa atgcgttaag gcgtattctg ctatcttcgc
    26761 tcgaaggtta tgccattacc tggattaaaa tacgtggcgt tgaccacgag ttttcaacta
    26821 tacccggtgt aatggaagac gtaaccgaga tagttctcaa cctgaagcag gttcgcttta
    26881 aacgaatagt tgacggtgtt gacaacgaga aggttaccat aacaattacg gggcagaacg
    26941 agtttaaggc tggctacctg tccaacttcc tttcaggatt caaggtgctt aaccctgaac
    27001 tggttatctg tcgcatggag cctgatgtta agattgagat tgaggttaac atcagcaagg
    27061 gtaggggata tgttcccgct gaggaaaaca agcctgaaga tgctgagttt ggtatcatac
    27121 ccattgactc cattcacact cctatccgta atgttaagta ctcgatcgaa aactaccgcg
    27181 tagagcagaa aaccgactac gaaaagctta tcatcgagat cagtaccgat ggctctattc
    27241 atccgaagga tgcactgaag gaagctgcta agattctgat ttaccacttc atgctattct
    27301 ccgatgaaaa gattaccctg gagaccgagg ataagtttga aactgaggaa ttcgatgagg
    27361 aaatcctcca catgcgccag ctgctgaagc aaaagctggt tgaccttgac ctgtcagttc
    27421 gcgcattgaa ctgcctcaag gctgccgatg tggaaaccct tggcgagttg gttcgttaca
    27481 acaagaacga cctactcaag ttccgtaact tcggtaaaaa atcgctcacc gaacttgagg
    27541 acttgctcga tagcctgaac ctctcgtttg gcatggatgt atcaaagtat aaattggata
    27601 aggattaatt acgatgagac ataacaaaaa atttaaccat ttaggccgta agagcgctca
    27661 ccgtaaggca atgctatcga atatggcatc gtcgttgata ctgcacaagc gtatcaccac
    27721 tactgtagca aaagctaaag ctttacgctc ttatgttgag ccactgatta cccggagcaa
    27781 ggacgattct acacatcagc gccgtgtagt tttcagccat cttcaaaaca agcaggcagt
    27841 aactgaactt ttccgtgagg tggctcagaa aattggcgat cgccccggcg gttatacccg
    27901 tattcttaaa actggtaccc gtattgggga cgctgccgat atgtgtatta tcgaactggt
    27961 tgacttcaat actacctacg tgaaggaaac agctaagccc gcagccaaga aaacccgtag
    28021 ggctggctca aaagctaaaa aggctgaaac cggtagcgct aacgtagttg ctgaagaacc
    28081 aaaatcggaa aatcaggata gcgaaaatgc tacttcaact gacgaaaagg ctgagtaaca
    28141 ccagtttaac aatactgatt tagggatgag cttttggctt aatccctttt tttatctaac
    28201 tttactaaaa actacaacaa actatgggac aaattgtaag cgttcatgcc cgtgagattc
    28261 tcgattccag gggcaatcca accgttgagg tggatgtgat tactgagagt ggatactttg
    28321 gccgtgctgc tgttccaagt ggtgcttcaa ccggcgttca tgaggccgtt gagcttcgcg
    28381 atggcgacaa gggtcgttac cttggcaagg gcgtgcttaa ggcggttaac aatgtgaaca
    28441 caattatagc cgatgagatt gttggtatga atgttcttga ccagcagctt atcgacgaaa
    28501 ccatgattaa gctcgatggc accccaaata agggtaacct tggcgccaat gcaattcttg
    28561 gtgtttcact agctgttgcc catgccgcag ctcagcttac caaccagccc ctttaccgtt
    28621 acgtgggtgg cgttaatgct cgtacccttc ctattcccat gatgaatatc ctaaacggag
    28681 gttcgcacgc cgataatagt atcgatttcc aggagtttat gattatgccc gttggcgcca
    28741 actcctttac cgaggctatc cgtatgggtg ctgaggtttt ccacaacctg aaatctgttc
    28801 tcaaaaagct tggatactca accaacgttg gcgatgaggg tggatttgct cctaacctga
    28861 aatcgaacga agaggctatc accgttatac tacaggccat tgaaaatgcc ggataccgtc
    28921 cgggcgagga tatttacatc gctctcgacc ccgcttcgtc cgagtactac ctgcccgagg
    28981 aaaaggttta ccacctgcat aaatcaactg gcgagaagct aacccctgct caaatggttg
    29041 attactggaa gaactgggtt gacaagtacc caatcatttc cattgaggat ggtatggccg
    29101 aggacgactg ggatggctgg aagcttatca cccaaacctt aggcaatagg attcagcttg
    29161 taggcgatga tcttttcgta accaacgtga accgcttgca gaagggtatc gataccggcg
    29221 taggtaacag tattcttatt aaggttaacc agattggtac attgacagag acaatcaatg
    29281 ccgttcgttt agccgacgtt cactccatga cctcaattat gagccaccgc tcaggtgaaa
    29341 ccgaggatac taccattgcc cacctggctg tggccttaaa cactggtctt atcaaaaccg
    29401 gatctgcttc acgttccgat aggattgcta agtataacca gcttatacgt attgaggaaa
    29461 tgcttggcga tgcagctatt tacccaggta agaacttcaa gtacatcaaa aaatagttcg
    29521 gtttttgtaa cttttcaaag gcttcggaag tattatctga agcctttttt tatttttagg
    29581 ggctaaatag ttcgggcatg gcctacaagt cgcttagtca ttttattgat gttctcgagc
    29641 aaaacggtga actactgagg gtgaaacgtt ttgttgatcc cgttctggaa ataaccgaaa
    29701 ttaccgatag gcattcaaag ctacctggtg gagggaaagc tcttctattt gagaatactg
    29761 gcactaattt tcccgtgctt accaatgcct tgggttccga gagaagaata tgcctggctc
    29821 ttggaaccaa taacttggag gcattgcgcg acgatgtggg taggctgctt gaacagttta
    29881 cacggccaag aggtagcctt tggtctaaaa tctcggcatt acccgaactt acccgggtgg
    29941 catcgtggat gccagcaagc cggaccggta agggcatttg ccagcaggtt gtccattaca
    30001 acccccatct tgacatgctg cccgttctta aatgttggcc acacgatggg ggtaggttta
    30061 taaccctgcc catggtacac accgttgatc ccaatactgg aactcggaat gttgggatgt
    30121 accgtatgca aattttcgac tcctcacata caggcatgca ctggcacaag cataaaactg
    30181 gcgctaagca ttttgaggag tacaagcgct taggcaagct aatgcccatt gcagtcactc
    30241 ttggtggcga cccggcctat acctatgcag ctactgcccc aatgcctgag aatgtggatg
    30301 agtacattct tgccggtttt ttacgtaaaa aacgggtaaa gctggttaag tgcctaacgg
    30361 ttgatattga agtacccgag gatgttgact ttgtaattga aggctatgtc gatccttccg
    30421 aggaaccggc atgggagggc ccttttgggg atcacaccgg tttttactcc ctggccgatt
    30481 ggtacccgcg ccttaaggtg acctgcatta cccatcgcgc cgatgccgtt taccctgcca
    30541 ccatagttgg tgttccacca atggaagatg cttatatagc caaggcaacc gaacgtatct
    30601 ttcttgaacc aatacgccga accctcctgc ctgagctaac ggatatggta ctgcctaccg
    30661 aaggggttgc ccataacatt gccctggtta aaataaagga tgagtttcag ggacaggctt
    30721 accgtgtaat gaacgcactt tggggtgcag gacaaatgat gttcaacaag gtgcttgttg
    30781 ctttcccaaa taacatcaat cttaccgatg gttaccaaat gcttaaccat atatgtagaa
    30841 ctgtaaaccc tcaatccgac ttgtttttcg ggaaaggccc actggatgtg ctcgaccatt
    30901 catcaaatca gtttgctttt ggtagcaagc tgtttatcga tgctactgcg cttaattata
    30961 gagaaactgg ttacagtatt agtatcgacg atattaaatc aagtacccta gcattttcaa
    31021 atcatatagt tgccttaaac ctgacactta ttgaaaagga catacctatt ctaatagcct
    31081 cggtaaggaa aaaggagata gaggattggc agttgtttaa acaggaactc acattacagc
    31141 ctatctttca ggatttgttt gctttagttc ttgttgattt ggaaaatcaa attgatgacc
    31201 tttcgctggt gctctggttt ttgtcgggca atatcgatcc ctcacgcgat ttatcaatta
    31261 cacgggttaa cggaagtaac ttgattttga ttgatggaac ccgaaaggcc tatcctaccg
    31321 ataactttcc aaggctttgg ccaaacattg ttaccatgga tggcgaaacc attgaaaggg
    31381 ttgatcaaat gtggcattgg ctcgatatag gtgaattcat tgaatcgcca tcgcttaagt
    31441 tctttaatct gaaccaaacc gatgatgcag taatgcgaat tcaataactt ttttgtacct
    31501 tagcctaaac catggggttg tttagaacag ataaaaaatt actgcctagt tgcgttgcag
    31561 ctcttttgct ggctgcaaca tcatcggttt ttgctcaaag ggtaaatgtt accctgttta
    31621 actccgataa tggtttgccc caatcgtttg taaatgcatt attacaggat tccttaggct
    31681 tcctgtggat tggcacccag gatggtttat gccgttacga tggttacgat tttgtggttt
    31741 ttaacaacaa cccctacgat agcctttcgc tctccaataa ctacgtaaat catttggttg
    31801 agggtgtaaa tggtgaactt tggatagcaa cccgtagtgg gcttaatagg gttaaccgaa
    31861 aaacagggaa ggttaaggtt tactacaatc atgcaaataa cccaaggtcg ctgtcgtcca
    31921 atatgatact tagggtgtac cgcgaccgga gtaataggat ttgggtaaaa accattgatg
    31981 ccttacattt atacaaccca acaaccgacg attttacccg atttccgcat ttcaacgata
    32041 tttttaacat atcacaaaca tccgatttgt cgccgatttt tgagaattca aacgaacgac
    32101 tttgggttgg cactaaggac ggactattct atttcgataa agaacgtcta gtttttaaaa
    32161 ggtactccga agatccagct aatccaaatt ccatttgggg taatagggtt aatgctatta
    32221 ctgagggttt aagcgggaaa ttgctaattg gtaccgataa ggggataaat gagtttgact
    32281 ttcttacaca gaaatttcgt agagtaatcc ttacgcttaa cgacccttcc aacaaatcgt
    32341 tgactactgg aattcagttc ctgggttatg ataacgattc ctccctttgg gttgggagcg
    32401 tgggaggatt gggtaaacta cttccaaacg gtacatttat taagtacaat agcatttata
    32461 gcaataattt acccgtaaat attttaaatg tatcaacggt gcttcgcgat aggacgggag
    32521 tactgtggat tggttcgcaa accgggctgg ttaaggtaaa cccctttgag caccgtattg
    32581 gtgggtacag taaggatcca aacgggcgga acctattctc aaacaacgtt gcagcatcgg
    32641 taattgaaag cactgacggt aatatttggg ttggaacatg gggtagcggt ttgcatatct
    32701 tcaaccccac aactggtgaa aaccgacatt actggtcggg aggaggcaac tacattccta
    32761 atgattttat tcattccctt taccaaacac gcgatggtag ggtacttgta ggcacacgca
    32821 atggggtttt tcaatatctt ccttcatcga ggcagtttgt cgactttttt gagaggaaca
    32881 atgtgtatgt tggcgatctg tttaagaaca acagggttta ctccataact gaggatttac
    32941 aggatagagt ttggtttgcc acccggcagg gattgcattg cttgaaaggt ggaaatctca
    33001 taagtttcta tgccagaagg ggtgattccc tttcgttacc gggaaatgag gtttacgatg
    33061 tggttgccga caaaaatgct aacgtttggg tggcaacaat ttcaggactt tgcagaatat
    33121 cagccgattt gagaaccatt gaaacattct cattcattgg caataaaagt aggttgcctt
    33181 tcgaagtgct ttgcctttac attgccaaga acgaaacagt ttgggttggt actaccagtg
    33241 gtttattctg gattaaccca aaaggaaaaa atgttctgca ttcggttgta ctacccgata
    33301 ttaatatcag gcttataaac gatattctgg aggatgatcg tggccgattc tggattagca
    33361 ccaataaggg attggttctg tataatcctc tttccaattt ggttaaggtt ttcaataact
    33421 ccgatgggct ttttagcaat gagctaaaca taaacgcatc gtttaagaat agtaaaggga
    33481 ggatgtactt tgggagtatc gacggtgtta acgtattcca tcccgattca attcctacca
    33541 ataaggttgt tccaaatgtt tgtattactc acattcaggt gtacagtaag tcgggcaaca
    33601 ccgaaataat cccgtatcag cttaagttcc taaggatacc tgatgatttt agcaacttaa
    33661 ccttcacaat tgcagccctt gattttgttc aacccaacaa gaattcgtac cgctatcagc
    33721 ttgttggtca cgatagccgt tgggttgagc tgggaaatac taacaaaatt actttttcaa
    33781 acctttctga aggcgactac gagttaagaa ttatgggttc caatagcgat caggtttgga
    33841 caacagaata taaatcgcta aggattcgtg ctgttgcgcc ttggtggcga tcgcggattg
    33901 ccaagtggat ttatggggtt attgtactga tttctctctt agtatgggcc attcgtagaa
    33961 ataagcattt gcgtgaaatt aaccgcttac tccatgaacg tgaaactgtg cttgaggaac
    34021 ttaggttgca gaaagacgag ttggccttta agaataagaa tatcaccgat agtattaact
    34081 acgcaaaacg tattcaggaa tcgatgcttc cttccattag ccgatttaag gaatttgtac
    34141 ctgaatcgtt tgtgattttt aaacctaagg atattgttag cggtgatttc tactgggtga
    34201 acgaaacccg taacaaaacc tttattgctg tggtcgactg tactggtcac ggcgttcctg
    34261 gggcgttcat gtcaatcatc ggtattgagt tgcttagaaa tatcaccaac gttgaaggcg
    34321 ttgacgatgc tgccgaaata cttaacaggc ttagcgtggc cattcataac acatttgcta
    34381 ccagtagttt tgatagtgct gagggtgtaa aagttaagga tggtatggat gtatcgttct
    34441 gtgttatcga caaggagtat aacatgttgc agtttgctgg cgccttttct aaccttttcc
    34501 tggttcgcga tggaaagcta atggagatca agggcgacag gtactccgtg ggtatggcaa
    34561 atgagctagg gcacagtcag ttcagcagct actacatacc aatccaacca aacgacacaa
    34621 tttacatgtt taccgatgga attgtcgatc agtttggagg cccggagggt aagaagttca
    34681 aataccgcag gtttagacac cttcttttga gtaactatat gaagcctatc gaattgcaaa
    34741 ggactaatat tgaaaaagcc attaacgatt ggatgggtaa ccttgagcag gttgacgata
    34801 tgctaattat aggtattaaa ccagagttaa gctgtatgtt ctagtattgt ttactggtga
    34861 taatgcctaa aattccagtt ctatttactc ttttacttta ccccatgctg ctttcgtttc
    34921 tggtccaaac cccatgtcca gaaacaacca ccaccgctcg attccggaat gcttaacttt
    34981 ttcttttttt gcttacaacc ccagaacagg ttaaagccaa accttaggtt tgcatttcgt
    35041 accctttccg ggtagaatgg ggcaagcagg ttatcggtaa gaatgtaaaa ctgaaagctt
    35101 tcacccccaa ggttaatagc ggctccaatg ttggcaaaag agccgttcat cacagagtag
    35161 gttaatgaaa ccgatgaacc tttcttggcc atagcaatgg ccgatagggt cagtcctacc
    35221 acaggcctgc ccggatacca ctcggttctt acatgtgcac ccgcaagtag ttgtggggta
    35281 aatgcgtaag tgcctccaaa ataggcttta gggtttaaaa atgacacaaa gctaccgttt
    35341 gagtgctgga attgaacctg attgtttatg gaatcggcaa gcatgtcgat ataggcatcg
    35401 gggttggtaa ggtcggcagg ggtggcacca cggtaaacaa aatggccatc gttggtaaag
    35461 cgcgaggtgc ttttgaacca ccatataaat cctaagtcca gaatgctacc actccatgta
    35521 atagggtcgt tgggctttac aaagcccaag tcgaatgcaa gccccggatt gctgaagtta
    35581 aggaagaatg gcaatgggct aaatgcccct gcgctaactc cggtaacatt ccctgcagca
    35641 tcggtagtta cccctaaggg gtaggctacg tttacctgtg gattccaacg ggcatcaata
    35701 ttgtgggtta aggcatctat ctcaatatca acgctattcc ttttggtgta aaggcctgca
    35761 agaccgaaca gaattttggc atgggcacca acaagcacat cgccacgtaa ccgggtggag
    35821 tagccaaatg aaaactccct gtagtaaaag ctgtttaggc cgggattgga ggttattagc
    35881 ccattgccca cgaactgcgt attcccgtta gcaaaaagcg acatcaaacg tttcgataca
    35941 aatagcttac tctccactcg ctcagtccag gcaaagttaa agtactggtc gttgtcgtac
    36001 ataaatccga acgaaacggg tgtgtagtgg agttcctccg ataggtagtc ccagctatgc
    36061 atttgcccaa caagggcagg aaagttaatg gtacctgttc cgtttgcaat atcattatag
    36121 gaaaaaccgg tagagttcag gtttaggtgt aacgacgaaa ggaaaggcac acctacatat
    36181 accggacaat caatctgaac tgctgggttt attaggttcg attgcggtat gcgatgcata
    36241 aagaaaagcg agttgctttg ctgtgcccat gccaatgtat aagcaaaaaa taaagcggtg
    36301 agagtggtaa atcgcaatag cataccctaa ttatttttga tagttttgaa atcgacacgt
    36361 gcagccagct gtacttttag cttgtaacgg tcaaaatatt ggatattctg ctcagtaaca
    36421 ccttcttcag taattaccaa tccggcccta accactgctg tggccgcgtt ttgccataag
    36481 tcgatacggt tttggtcaaa atcaacctga tacgattcaa acttggtgcc caccacgttg
    36541 ccatttgagt atatctcggc tgcaggaaca ctggccggtt gtgcaaacag tgagtctaac
    36601 ttattttggc cattgtcaag tatccatatt tgggcatttc cttgtagtgg aaactcattc
    36661 caaatgttaa cgcgaatcat tatgtagcta atcagatcgg actgattgta aacctcattt
    36721 agattaattg caaaggtatc ctgtatgtaa acatagctgt gttttgccca gtcagggaca
    36781 ttctcggtca ggtttacaac tggtacccca agggtttcaa gcgatttact gagattaaat
    36841 tcaccctcac caaccggtaa ggaaattgaa ggagcaaagc ttaccgtatc gggtaaaggt
    36901 tcaatgtttt ttgttcagca cgtgaggttt gttatataaa caaccaatat gctggttttt
    36961 accccaatgt taaatatcat tctcatttaa tagtgttttt ctttaactat aacgcaatgc
    37021 aattaatttt atgtttctat atcccgtatt cgccaattga aaaatctaat gatatatcga
    37081 ctaaaaaatc taatcattcc gactcaaaat cactcgttta acgcttcacg gttcacgttt
    37141 aacgcactca agtttcctct aacttcctct aacatccttt agcttcttct tactccgatc
    37201 attccgatca ttccgatcat tccgatcatt ccgatcattc cgattattcc gactcattcc
    37261 gacccaaatt cactcgttta acgctttacg gttcacgttt aacgcactca agtttcctct
    37321 aacatcctct aacatccttt aacttcttct tattccgatc atttcgatca ttccgactca
    37381 ttccgattat tccgccccaa agtcactcgt ttaacgcttc acggttcacg tttaacgcac
    37441 tcaagtttcc tctaacttcc tctaacatcc tctaacatcc tctaacatcc tttaacttct
    37501 tcttattccg accattccga ttcattcctc taaaaaccac cccctcccca ttttatcagc
    37561 ccagttgagt ttttagttca tgaaacttca gtttaacatt tttataaagc attgtaattt
    37621 acgatatttg attaaatggt caagtgtggt gttgataaaa aattgaagca taatgataaa
    37681 tggctaactt tgtaattcta aaaggagatg caagtcatgg caggattcaa gttttttagt
    37741 ataccaaaac ccaagggttt taggtataag cctatatact acaatgagca aaaggaggag
    37801 atgcgtgagc gcgaggaacg agttcgaaag gaattggggc tcaccgataa ggataaacct
    37861 tttgtgccaa gcattagggg acagtttcaa cgtgcacgaa aaattagcag accttcgagt
    37921 aggcagtcga atatcaggtt attgattatt attgcaattc ttctgctaat cagttacttc
    37981 gtatttttcc gctagtatgg ccgatgtaat tcaactgtta cccgattcca ttgccaatca
    38041 gattgctgcg ggcgaggtgg ttcagcgtcc ggcctcggtg gttaaggaac tcctggaaaa
    38101 ctccatcgat gctggggctc gtaatatcaa cctaattgtc agggagggtg gtaaaggttt
    38161 aattcaggtg attgacgatg gggtgggaat gagcgaaacc gacgcaaggc tttgctttga
    38221 gcgccatgcc acctccaaga ttcgcgaggc agccgacctc tttaacatcc gaaccatggg
    38281 ttttaggggc gaggcactgg catccattgc tgctgttgcc gaggttgagc tgcgaacccg
    38341 ccgggctgtt gatgaggttg gaacggaggt taacatttcg gcttcggaac tggttagcca
    38401 gcaaccggtg gcctgttcta ccggcaccca aattgcggtt aaaaatctct ttttcaatgt
    38461 gccggcaagg cgcaagttcc ttaaaagcga ttccgtggag cttaagcata ttataaccga
    38521 gtttcagagg gtcgcattgg cttaccccga agttagtttt agtttgagcc acaatggttc
    38581 agaactctac aggctgccag catcgggatt gaagcaaaga gttattggcc tatttggtaa
    38641 gaccataaac caacaccttg tggattgctc cacgcaaaca tcaatagtta atattggtgg
    38701 ttatataggt aagcccgaag gggctaagaa aactgcaggc gaacagttct tttttgtgaa
    38761 tggccgtttc atgaagaacc ctttcctgca caaggctgtg atgaacgcat ataaccgctt
    38821 actgccaccc gatacctacc cctcatattt catttacttt gatatcgatc cgcagtccat
    38881 tgatgttaac atacacccta caaaaaccga aattaagttt gaggacgagc gaatgatctg
    38941 gcagatatta cacgctgctg tacgggaatc gctcggaaaa ttctctgtgg tgcccccaat
    39001 cgattttgat tctgctccag gattcgatat cccatacctt cccaagaatg caccggtaac
    39061 ttttcccacc attgacgttg accccaccta caaccctttc gatgagcata acaggaatgt
    39121 tcgtaagcct catagcgccg ataaattgcg tgaatcggca accggctggg agcaactttt
    39181 taccgattta ccccaggatc caacccccat tcaaacccga atcgaaacct ttgaatcgga
    39241 agtgatgctg agtaaagctc ccgaaaacac caaccgtttt taccagttca agggcaagta
    39301 catacttacc accgtaaagt cggggctgat ggtgattgat caaaaacggg ctcatgagcg
    39361 catactttac gagcagtata taagcggcat gcaagccgat ataaacctga aacagcgcga
    39421 gctttttcca caggccatcg agctgctacc tgccgacttt gagctgctga tgagcaaggc
    39481 cgatgaactg tcgcgtttgg gtatggaaat cagcaaccta aaccacaaca caataagcgt
    39541 gaatagcatg ccggttagct catcggttac cgatcccgca aagctggttg aaaacctgct
    39601 tgccattatg cacgagaacc aagccctccc attcgacgat gctaaacatc gcattgcgct
    39661 taccatggcc aaagcatcgg ctattggata cggtaaacag ttgagtcagt ttgaaatgca
    39721 ggagatagtt gataagcttt ttgcatgcca tgaaccaaac cttacccctg atggtaaaaa
    39781 gattattgta attcttgaat tggacgatat tgacagccgg tttaagtaaa ccctaaacat
    39841 tttctgatta ctaatgttaa ctcaattcat aacactaata agatgatatt cggaaacaga
    39901 aatattttta gctcaacacc ccctgtggtg atgaacctaa ttatgattaa cgtggtaatg
    39961 ctggtagtaa cctgggtgat ggcgagcacg tttggaattg atttaaatcg tatccttggt
    40021 gtttatcccc cgggctcacc cgactttcgc ccttaccaga ttgttaccca catgttcatg
    40081 catggagggt tattacacct cttcttcaac atgtttgccc tttggatgtt cggtcgaatc
    40141 ctggaacagg tttggggtag ccgccgcttt tttatctact acatggtaac cggcctaggg
    40201 gctgtaacca tacatctttt ggttaactac ctccacattt cggctatcca gaacgatgct
    40261 atggccatgc taaacacccc gtcgcccgat gcttttgctg catttgttac ccgccatttc
    40321 cctgagtact acgatcaggt ttatgcccag tttctggata aatggtactt gttcccaaac
    40381 agcccggttt atgccgatca ggctgctgag tacacccgtc aactgattgc gctgcagcag
    40441 ggcataccaa ccgttggagc atccggtgca gtttacggtg tgctgcttgc ctttggggtt
    40501 ctgttcccaa acactatact gatgttgctt ttcccgccta tcccaattaa ggctaagtgg
    40561 atggtaatta tttacggtgg tttggaacta tacctgggtt taacccaacc tgggagtagt
    40621 attgcccact ttgctcacct gggtggtatg atttttggct ttattctcat caagctttgg
    40681 caaaggcgca ccgatgtttt ttattagtgt tgatatttag ccgaaaatcg gttaacttta
    40741 ctaatacctt taaagttcgt tttttgaacg gtttaacatt tttgtgttat gactatttgg
    40801 gatgagatta agagttcgtt tcggctgggt agcaacctta cccggcttat ctatatcaac
    40861 cttggtgtat ttttactttt tggtttgcta agggttttcc ttttcctgtc tgcaagttct
    40921 tcgggctgga taacaggttt tatggcagtg ccggccaacc ttcaatcgct catggttcgc
    40981 ccatggacta ttgttaccta catgttttac catgaggatt ttttgcatat tctctttaac
    41041 ctgctatggc tttactggtt tggccagctg tttctgcaat tctttgccca gaggcagctc
    41101 acatgggttt acctgcttgg cggtatcagt ggggctttac tatacatttt agcgttcaac
    41161 ctgttacctc catttaaagg ctatgctgag gtatcgttag ccttaggcgc atcggcttca
    41221 gtgctggccg tggttattgc agtggccaca cttcagccca actttactgt ttacctgctt
    41281 tttcttggtg cagtaaggct aaagtacctt gcccttgcca ccgttgtcat cgatatcatt
    41341 agcatccctg tttccaacgc tggaggacat attgcgcacc ttgggggagc cctgtttggc
    41401 tttctctacg ttgctgctct gcgtagcgga accgatttgg ctaagccatt ttacagcatt
    41461 agcagcatta ggtttccaaa acggaaccga atgaaggtaa cttaccgcag ccataatgcc
    41521 gattacgact acaactaccg taaagcacaa aagcaaaagc agatagatga tatcctcgat
    41581 aagattgccc gctccggcta cgatagctta accaaggagg aaaaggacat tctgtttaag
    41641 gccaataaca ggaacattaa ttgatgctca ttgtaaggct aatccatcgg attttggtgg
    41701 gggtaaatgt actttttgcc ctcttgctgg tgcttaccta tgtgagcgtt tacataaacc
    41761 ctgaagattt ttggtttgtt gcgctatacg gattgcttta ccctttcttt ctgatagtaa
    41821 acctactttt tcttttttac tggatttttc gttggcaaaa gttagttttc atttcacttg
    41881 cagccattct gcttggcgtt agccatttca atggctttat tcgctttccg tatgggagtg
    41941 ctgcaaaggg ttccaatcct gatttaaggg tgatgactta caatgtaaac ctttttaggc
    42001 tttactcgtg gtcaaagctg aaaccctcat attccgatat cctaactcat attaaggaca
    42061 acgactttga tgtggtttgc ctgcaggagt tttatcttag ggagggcaag ctgagccatc
    42121 ttgatgttga gcagcagtca gggatgaagg tttactcctc gttcatactt aggcggccat
    42181 cgtcggctta cggcttggcc attttaacca atcaacctgt tgtgagtgtg ggcgaaatat
    42241 cgtttcccaa ttcgtttaac gcatgtatgt atgccgatat agttaaggga accgatacca
    42301 ttagggttta caaccttcat ctgcaatcaa cccggttaaa ggagcgtaac tttaactttt
    42361 tgcttcgcaa cgagtttaag ctcgatagca agaactacga tgagctaaag gatttactca
    42421 gccgattagg cgttgccttt acaaagcggg cccgccaggt gaatatggtg gttgaacaca
    42481 tgcgtacttg ccgttacccc attattattt gcggcgattt taacgattcg cccgtatcgt
    42541 acacctacca taccctaacg gcttccctac acgatacatt taaagatgca ggtagcgggg
    42601 ttgtagtcac cttcagcggc ctgtggccct cgtatcgaat cgattatgtc ttacggagta
    42661 gacagttccg aaccgtaacc taccaaagcc cacggctgag gtactctgac catttcccgg
    42721 ttgttgttga actgcaacta cagtgaagcc gttagccctg ccagggcaga tggcttaaat
    42781 ccacattacc ccctgaaatt attacgccaa cacgtttgta gcggaaaagt tgagcattct
    42841 ccatgatggc cgccaccggc acagccgacg atggctcaat aataatcttc atgcgctccc
    42901 acaccatgcg catggcctca ataattgatt gttctgatac agtgagtata ccgtcgcagt
    42961 gctggctaag aatggtgtag gttaggggcg aaagcgatgt gagtaaccca tcggctatgg
    43021 tttggggttt taccgagggg taaatggtat ggtctcggag cgagcggtag gcatcgtcgg
    43081 cattctttgg ctcagcacca taaaccttaa catgcgggaa ccgccctttg ataaatgtgg
    43141 cagttccgct aagtaatcct ccacccccaa caggcgcaat aacaatatcg agctcaggct
    43201 cctgctgcat aagttccagt gctgctgttc cttgtccgca caccacgtta aagttatcgt
    43261 aggggtgaat gaatgtggct tcggtttggg actttatgcg gagcatggtt tgctccctat
    43321 cggcttgcgt cggggggcaa aaggtgatta tccctccata ccctgcaaca gctttctttt
    43381 tgatttcggg agcattttca ggcataacaa tgtatgccgg tattcccctt tgccgtgctg
    43441 ccaatgccaa cgctgcagca tggttgcctg aactgtgggt gataacaccg ttgtgagcct
    43501 catcgctacc taaacgcaga atggcatggg tggccccacg gaacttaaag gctcctaccc
    43561 gctgaaagtt ctcgcattta aggtaagcgt tacaccctaa tatggtgttt acactcctgg
    43621 atgttagaac cggcgtgcta tttatgtgtt tcgcaataag attaaaagca ttctcaatgt
    43681 ctaaatggct tggagtggtt acatcctgca ttgccataat gtttactttt tggtgttatt
    43741 ttttcctttt ttaattgttt caaattcctt gaacctatca agtatccgtt cacgtatttc
    43801 gtaatcggtt gaggttatta taaagttctc gtcgaacttt aggtattcca taaattcctg
    43861 cagttcattt ccggttaaac cggttaggct gctaactata cccgcattaa attttcggtc
    43921 ggctattcgc ttttttctat ccttttcaat ggcctcggca accttttcgt tttgcttctg
    43981 gtaccagtcc ttgccaaagc ttaaccccat ggttggctgt ggtaggttac gcaggtttaa
    44041 gcccttggtg atcttaagga agtatagctg tattacctcc tgctgctccg ttacccttgt
    44101 ttttgtcagc tttaggttgg caaactcgcg tagaaatcgg tcgtaggtgc tgaaacgcga
    44161 tattttaacc tcttcaatct ggtaaacctt tggggtaagg gttagctcgt aaaaaagggt
    44221 atccttgtta aactgtcccc agttcagcac atgcttggtg tggtagccta tagcagtaat
    44281 gctaaggcta tcgcccagct taaatggaat gctaaagtaa cccagcaggt cggagataca
    44341 accctttttc tgggtgatat tcataacatg ggcaagccca attggctcat gggagtagta
    44401 atccgtaacc ctgaaattga taacgcgcaa cgagtcgggc accgg
//
LOCUS       DAOOWJ010000006        42162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_969_length_42162_cov_8.472938, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000006 DAOOWJ010000000
VERSION     DAOOWJ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 42162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42162
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_969_length_42162_cov_8.472938"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            630..1745
                     /locus_tag="PLW22_01325"
     CDS             630..1745
                     /locus_tag="PLW22_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73372.1"
                     /translation="MRNIVFLSALMVLQHFVYAQEVNDSIIYHQNQRILQFLESSKIS
                     TQIFLGYRYYEEGNGNYNEFVVKRGYITFDKSLNKYLSGRIAPDLTIDKEGDGEGDLE
                     MRLKYCYVELKDEGSYGVFTSPKLLIGEVPTPFIDFEEKVNAYRVVASQYLDDMKILT
                     SADFGVTTSVLLGGKIDEGYQKTVSNAYPGKFGSIAVGVFNGGSYHALEYNNNKTFQW
                     RLSVRPMPERLPGLQFSYAGAHGKGNTALYPDWDTHTGFLSYESRRLVLTGQLFRSLG
                     NHEGTLADSLGNSYTAKGLNAFLEVKFLKNRFSLFGAYGVMRYTDYAGTDIEKTREII
                     GLAYHIYKKNKFVIDLNRSISKGKCKGVIEFMFELAL"
     gene            complement(1824..3494)
                     /locus_tag="PLW22_01330"
     CDS             complement(1824..3494)
                     /locus_tag="PLW22_01330"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOU73373.1"
                     /translation="MKSDIEIAHEAKMKPITEIAGILGINPDDLIPYGKYKAKIPLSY
                     IDKEKYQQNKLILVSAISPTPAGEGKTTVSIGLAQGLNRIGKQTTVVLREPSLGPVFG
                     IKGGATGGGYSQVLPMEDINLHFTGDFNAIEKAHNLLAALIDNNLQSKKRSLGIDPRT
                     VHWKRVMDMNDRSLRHIIVGLGGTTHGVPRETGFDITAASEVMAILCLAENMEDLKQR
                     LGKIFVGYTYDKKPIYARDLHAEGAMAALLRDAIMPNLVQTIENTPAIIHGGPFANIA
                     QGTNSVIATKMGLSLSEFVVTEAGFGFDLGAEKFIDIKCQYAGLKPSAVVLVATIRAL
                     KYHGGKPLKEVSQPDVEALKKGIGNLQKHVENMKLFGICPVVAINRFTTDTDEEVEYI
                     KSVCREMDVQVAEVDVWGKGGEGAIELAKIVNKIAMNCPYQHKTLYDWNWTPEAKIET
                     LAKKIYGAEAVDYTAQAKEDLKKVYSLGLDKLPICMAKTQKSLSDNPDLLGRPKDFVV
                     TVREIEVAAGAGFIIPITGQIMRMPGLPDEPAAERIDVGNNGVISGLF"
     gene            complement(3614..4621)
                     /locus_tag="PLW22_01335"
     CDS             complement(3614..4621)
                     /locus_tag="PLW22_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73374.1"
                     /translation="MRNKWIVAAITVLAIQGCKENRFDSIQPPRDVEPVSITRFEKEL
                     FSINPDSIEKAFPIVYQKHTSFTKLFTEGIIRIGKNDSPDFYRYLGLFLTDTMVQNAN
                     RMVESVFPANAPFEKEISDAFARYSFFFPSKPLPKVYTYISGYNLSLAIDDSLVAVGL
                     DRYLGRNTIQYSLLGIPVYQQRKMHPGKVPSDVMRAWLYSAFPFHDSINNLLSNMIYE
                     GQIMYLTKRMLPYQPDSLIFGFTPQQYKWCLNNESAMWTHLIENKLLFTTNTVDINKM
                     INDAPFTSGFPQESPGRAAVWIGYRIVERFMERNKNIDLQRLMLIKDYQEILTLARYK
                     P"
     gene            4882..5139
                     /locus_tag="PLW22_01340"
     CDS             4882..5139
                     /locus_tag="PLW22_01340"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HOU73375.1"
                     /translation="MIDVAQVFGLLGSITAASLFFPQVYTSWKTKKTHDLAWSGIAIG
                     LLNGVFWIVYGLIRLDPFIYVTNTILFIGAFLLLILKKRYG"
     gene            5385..6872
                     /gene="dnaB"
                     /locus_tag="PLW22_01345"
     CDS             5385..6872
                     /gene="dnaB"
                     /locus_tag="PLW22_01345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOU73376.1"
                     /translation="MVSELGKVPPQAVDIEQAVLGAIMVEKDGVIIASEILKPECFYK
                     EAHQIIFKAIQDLSARLEPIDIFTVTEELRKQSKLDEVGGAAYITELSSKVGSAAHLE
                     YHSKIIAQKYIQRELIRVATDIERRSFEDDIDVDELLDYSEMELFKVAEGNIKSDTQP
                     ISNIVVKAFKQIEEAGKREDGLSGVPSGYTDLDRLTSGWQKSDLIIVAARPSMGKTAF
                     VLSMARNMAVEYDVPVAFFSLEMSNIQLVNRLIIAESGIPSEKIRNGRLSNDEWTQLT
                     VKTKQLADAKIFIDDTPALSIFELRAKCRRLKAQHNIGIVIIDYLQLMTGPTDTKGNR
                     EQEVSTISRSLKAIAKELDIPIIALSQLNRSVETRGGNKRPQLSDLRESGAIEQDADV
                     VIFIHRPEYYGFDVDDSGNSTLGIAEIIVAKHRNGAVDDVRLRFQKEMAKFSDLDDMT
                     IIPNTITPTYTSSAPVTFSSKMNNDPVDDFTFNPGKGIDNTNIPF"
     gene            complement(6875..7591)
                     /locus_tag="PLW22_01350"
     CDS             complement(6875..7591)
                     /locus_tag="PLW22_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOU73377.1"
                     /translation="MILNFFFKHNAYKHIFFDLDRTLWDFEQNMRDALAEIYANHKLD
                     LAFPNPDTFIDCFNRNNHYLWDKYLKGELTKEVLRYKRFEITLAEYGIESKPLAETIG
                     DEYLTIMPLKTALIPGTRETLDYLANRYKLHVISNGFKEVQLPKLQRSNIDKYFEWVV
                     TSEHSGFHKPDKRAFGYALSKANARKGESIMIGDDLEIDILGAKKFGMDQIYFNPKSN
                     PHQAKVTFEVRELVEIKKIL"
     gene            7929..9815
                     /locus_tag="PLW22_01355"
     CDS             7929..9815
                     /locus_tag="PLW22_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOU73378.1"
                     /translation="MSTSNPTIKVLMVDAASGFYKVNRFQVGDFFGPIDLGIHLSHKH
                     NSLNIGVGLLAGSIFPGSNRLIVNGISPCWHGFYISSMGGAGLVFDNLGINMFSIVGK
                     AAAPSILYLNRIHGEEIEVELLPVRPRTIWAEGRGGVYSMMQYALNQFGSRYENEPRI
                     LATGPASMYTDFGAIASAPIRKNEITYVDTWAGRGGFGTKMVQQHNIVAIIYGGTYID
                     EDFRDRTVADSWFEDRYQKRLAAKDMEATTKYRFDPKFETGGTFGVNYATMKGDIMAF
                     NYRTIYMTEEERLKIHNDFILNHYLKQFNEETIKTKQQRTCGEPCGAVCKKMHGEFKK
                     DYEPYQTMGPLCGIFDQRAAEKLNHHADMLGFDAISVGGVLSWLMDCMADGLVEPQEL
                     GVADIPVFTPNGFRVVEDSMHNANIGIALLDQMVNPNGVFHLREGARKFARHLARRKG
                     TSVLDKFIYIGFARSGWMVPNQYWTPGVLSPMAIMGKYYMYYGKDFVPPRQLGRENAK
                     RMLNELVMDNLGMCRFHRAWAEDILPDVIEQLYGLKEKFQRSILLTASRINSRNASIF
                     WESERNIDFVYSFLRKKKEVDGASNPELDRWIKYFEEDKRSAALDFWYEIHKGVHESL
                     LEFH"
     gene            9837..10214
                     /locus_tag="PLW22_01360"
     CDS             9837..10214
                     /locus_tag="PLW22_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73379.1"
                     /translation="MKIIRRILFFIALLLVAIAAFLYWGTYEEGVMAGKIIRISQKGL
                     IFKTYEAKLGLESFGALRGTSPIAETFDFSVEPSNHELVKELEQVALSGERVNIHYKK
                     RYIRVPWRGSTKYFATRVERLQR"
     gene            complement(10269..10643)
                     /locus_tag="PLW22_01365"
     CDS             complement(10269..10643)
                     /locus_tag="PLW22_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU73380.1"
                     /translation="MNIYVSGLSYRINDDDLRQLFEEYGQINSAKVITDRETGRSRGF
                     GFVEMANDEEARTAIEELTGAEYDGKVISVSEAKPKTDKPRNGGGFNRERRGGGYGER
                     RGGYSREGRDSRGGGRGGRRDY"
     gene            complement(10979..12787)
                     /locus_tag="PLW22_01370"
     CDS             complement(10979..12787)
                     /locus_tag="PLW22_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU73381.1"
                     /translation="MLKNHPKGLLVAFFSNMGERFGFYTMMAILVLFLQAKYGLSAEE
                     AGGYYSWFYFAIYALALIGGLIADATNRYKTVILLGIIIMFGGYAMIAMPGMSLTMTL
                     LGLFIIAFGNGLFKGNLQAVVGQMYDNPEYSKVRDTGFMIFYMGINVGAFFAPFVATG
                     IRNWFLRSQGFEHDASLPSLCHAFLNGKVTDPESLAKFESLASKVSGATITGTEQLTD
                     FAHKYIDAFSRGYNWAFGVAAVAMVISLLVYVIFNKKLPNKEKKVSTTPAAGSLFTKK
                     NLLNVIVPIALVLLTGAIFWLLGAEIALGLAIGLFFGFVAMMFMMSTKEELPKVTSLL
                     LVFVVVIFFWMSFHQNGLTLTLFARDYTVKEVGPFTNIFFNLWSMLSFIGTIAGLVLM
                     FARKQIRERVIGLIMFLGLGYLTYYQAIKFGASNPIAPEVFQSFNPLFIVVLTFPVMG
                     AFAWMNKRNIEPSTPKKIGIGMIIAAIGFVVVLLSSIHLIAPKTIEAQGLTEWGRVSP
                     YWLISSYLILTIAELFLSPMGLSFVSKVAPQRFQGLMQGGWLLATAIGNKLLFVGSFF
                     WERIELWQLWTIFIVCCLLSAIFIFSIMRRLEEATK"
     gene            13367..14881
                     /locus_tag="PLW22_01375"
     CDS             13367..14881
                     /locus_tag="PLW22_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOU73382.1"
                     /translation="MKVFKTSQIKSIDAYTIENEPIESIDLMERAATAIFNWFRSKYH
                     KNRRIKIFCGPGNNGGDGLALARLLINTGFNAHCYYLKADSYSADFLTNLDRLKELTP
                     ELFLVKHQSDFPEIHTDDLVVDALYGSGLSRSIEGVAAQLIEHINSSGAKVIAIDIPS
                     GLFGEANPVPNNNPVVKSDYCLSLQFPKLAFFMPENEQFLKRWVVLPIGLHTEAIENT
                     ATPYHYTTASSIKTDFFQLSTFAHKGTYGHALVIAGSNGMMGAACLCAKSVVRSGVGL
                     VTMHIPKVGYTIAQVNVPEALTSIDSCETHITQFPELYRYQAVCVGPGIAQSTETDQM
                     LVKLIKTCQLPLVVDADGLNLLSRNIDVLNSVNNALILTPHPGEFDRLFGKSQCSWER
                     LERAREMAQKYGVIIVLKGAYTRVVLPSGNVHFNSTGNPGMATGGSGDVLSGIITGLL
                     ARGLPPADAAITGVYIHGLAGDLAADEFGQISLCASDIVNYICKAFQHITNNDE"
     gene            14874..15971
                     /locus_tag="PLW22_01380"
     CDS             14874..15971
                     /locus_tag="PLW22_01380"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOU73383.1"
                     /translation="MSKNLFLAVLSLVLLGCNSKTIQVEPAGKTIDYKKVAVYYSLPR
                     TVVYVKVVSSQSEYFPGPYADYAKKFLGIDGVSNTEKVEYLIEKIDVETTAEPDPKAV
                     FAIYPSGKGYGNFINLSNEGLILPVNQTPESRAIAKNKEVSSKGEVIYKDLSVNPFVA
                     EESTTFYGRTLRDSVYVRVPIQRSMTIERNLEEKAKEAADFIFSIRKKRLDFLTPDID
                     HPFSGDALKLILKELQRLEDEYLALFVGKRFTHTFTQTYSFIPSNTEGESAILFRFSE
                     SKGLLSSTDLSGRPILIEFDQPEYPENIDATKNSLETIALKAKIDRFYYRIPVAVNMR
                     VIDGKQLLVEKRLSIFQYGLLAQLPVNFPGK"
     gene            complement(15972..16526)
                     /locus_tag="PLW22_01385"
     CDS             complement(15972..16526)
                     /locus_tag="PLW22_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOU73384.1"
                     /translation="MKNRIWVLTIWMLGISASVSIAQETKINWIEIDRAFEVNEKTPR
                     KIFIDVYTDWCGWCKKMDENTFTNPVIASYLTQKFHAVKFNAEGSKDITYKGKVYKNK
                     GQGQGQRNPHEFAIAILQGRLSYPSIVFMDENNNLITYIAGYLTPEQLEPIMAYIESD
                     AYKTQKWEDFRAKFVSKLNTVANP"
     gene            complement(16577..17104)
                     /locus_tag="PLW22_01390"
     CDS             complement(16577..17104)
                     /locus_tag="PLW22_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOU73385.1"
                     /translation="MAIIKPLRGITPKIGKNCFIAETAVIIGDVEIGDDCSIWYGAVL
                     RGDVNSIRIGNRVNIQDNAVLHTTYQKTTIEVGDDVSIGHGVILHGAQVSRGALLGMG
                     CTVMDNAKVGEGAIVAANALVLENTVVEPACVYAGVPARFVKKVAPELSRETNERIAR
                     NYLFYSGWYKDGDAK"
     gene            17175..17900
                     /locus_tag="PLW22_01395"
     CDS             17175..17900
                     /locus_tag="PLW22_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOU73386.1"
                     /translation="MNIRVYGDLNDFLPQGKKHKDQRVGFFGMPTVKDLVESIGVPHV
                     EVFLILADSLPVKFDYRPANGTLISCYPKFTSLPLSAIALRPDFEKPPRFILDVHLGR
                     LAAYLRFCGIDTLYDKSFNDNQILDIAQADGRIILTRDKGILRNGQAQFGYFLRNTNP
                     QLQLAEVVHYFDLAHYLDPFSRCSLCNGEVEQVDKSMVKNLVPSTTYQIYSEFFQCSR
                     CGQVYWEGPHFIRISKLLSRYSE"
     gene            18084..19937
                     /locus_tag="PLW22_01400"
     CDS             18084..19937
                     /locus_tag="PLW22_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOU73387.1"
                     /translation="MDKQKSVQELDEVVVRFSGDSGDGMQLTGTLFADASALFGNDVS
                     TFPDYPSEIRAPQGTIGGVSGFQVHFGNRKVNTPGDFCDVLIAMNPAALKANAKWVKR
                     GGMIIVDADQFNEKGYQRAGYKTFDAVSELNLQDYKVIFAPITNLTKESLKDSGLDNK
                     SIIRSKNMFALGMVYYIFDRPLNFTEKFFEKKFSKKPEVVEANKKVLRDGFNYAANIQ
                     AIASTYKVKQATTLPKGKYRNINGNTATAWGLIAAAEKAGLPLFCGSYPITPATEILI
                     ELAKRKDLGVKTLQAEDEIAGICTAIGAAYAGNFAVTTTSGPGLSLKSEAMGLAVMTE
                     LPLVIVDVQRGGPSTGLPTKTEQSDLMQVLWGRNGEAPVVVIAASTPSNCFDYAFMAG
                     KIAMEHMTPVVLLTDGYIANGSEPWRIPSMKDYPEIKPPIVPEGTEGYMPYARDEKRL
                     ARGWAFPGKKGIAHRVGGLEKDFLKGTVSHDPTNHQRMVDIRAAKVQRVQEFIPEQQV
                     IGDPEGDLLVIGWGGTFGHLFDAVQEMRANGKKVSLCHFNYINPMPKNVADIFGRFKK
                     LVVCELNMGQFANHLRMNYQQFNYLQYNKVQGLPFTVVELVEKFNQILEGK"
     gene            19940..20968
                     /locus_tag="PLW22_01405"
     CDS             19940..20968
                     /locus_tag="PLW22_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOU73388.1"
                     /translation="MEEQYMNYTPQDFKSDQEVRWCPGCGDHAILNAVQKALPEVSKQ
                     LKYNLERYVFVSGIGCSSRFPYYVNTFGFHGIHGRASAIATGVKVANPTLSVWQVTGD
                     GDALAIGGNHFIHAVRRNIDINILLFNNQIYGLTKGQYSPTSPLGAITKTSPFGTVEH
                     PFRPGELVIGAQGKFFARSLDVDVKLTAEIMVEAAKHDGTSVVEILQNCVIFNDGAYD
                     YITDREHRDDRVIVLRHGEKMVFGKNRDKGLILSGLSLKVVTIGQGGFTMDDILVHDA
                     YNPNPGVHMMLANMEYPDYPVALGVIRNVKDSTYDDNVRDQVLEVMKTAKIKSVDDLL
                     NSGDTWEV"
     gene            21130..23604
                     /locus_tag="PLW22_01410"
     CDS             21130..23604
                     /locus_tag="PLW22_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU73389.1"
                     /translation="MVKQKFIKALIALQFILSFSVSEVKAQDSFKQIFSDAEYYFFLQ
                     DFKEALPLYQSLYQQDSTNANILYKLGMCYLNIPGLKQNAIPYLEKASKNVNPKYREG
                     YYRETAAPIQTYFYLGQAYMVNFKFDDALLAFQKFKDNIDVKDVYNLDYVNQQIKACE
                     VAHNFISRPIVMKTEHIDLFPDRNKNCNYPVISGDRQTMVFTVKEKFYTAVYYSRWID
                     GKWSSPRNITLDLRVEGELYTTALNYTGDYLILFVNEVTSGNLYYSTLVGDKWQPVKK
                     LPAPINSKDWETFASLSVDGKQMYFVSNRKGGYGGTDIYMSSLQPDGKWSNPINLGPQ
                     VNTPYNEESPIVCPDGRTLYFASQGHNSMGGFDIFYSRKIDGNSWSMPINLGYPFNTP
                     DDEFYFYPLDSLSGVMPMAISNQSTFYELYKVNIYPSISRKIELFGKVNLSDNADIKS
                     DSIAILVKDTANNLIAMALPLSDGTYSVAIKPGRYSLEATSQFYVMNPLQLHIPTTYN
                     QEKYLLDINLDAKPITKEEVIRFNYVLFDFDSYELKRDAQFELEKVYKLMTDYPDLYI
                     EVIGHTDSKGSPMYNLMLSARRANAVAEYLVNKGIDEKRFVVRGMGSLVSFAANTNPD
                     GSDNPNGRKLNRRASIRVFNPNKNLKIEFVDVPEHLKPQTQNYTIMLAPIDDTISPDL
                     IKAIEKKFNINLREFVIGSRRLVCMNVYKSKADAIEHLNTIIDMGASRAVLVNEVELQ
                     RLVAALQKNLITKQSVFTILVATSEIPLTLDFFRGLYVTEEVGNDGLYRYYFGAFNEK
                     AKATEMLEKVNSMGFPNAILVKLEKR"
     gene            23604..24170
                     /locus_tag="PLW22_01415"
     CDS             23604..24170
                     /locus_tag="PLW22_01415"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOU73390.1"
                     /translation="MEGNVLENSVVKLRAPEPEDVDLLYSWENNMEIWKVSNTLTPFS
                     KYLLKKYIETSHLDIWESKQLRLIIEAKDQNSLMNVPVGLVDLFDFDPYHLRAGVGVL
                     IAKNEDRRKGYATEALNVLIRYVFEVLQLNQLYANVSASNVISLRLFENLGFTKVGVK
                     KDWLKTLNGWEDEVLLQLLNPKPRLPKS"
     gene            complement(24198..25658)
                     /locus_tag="PLW22_01420"
     CDS             complement(24198..25658)
                     /locus_tag="PLW22_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU73391.1"
                     /translation="MSNLEKYFEQFRKNIVGIDATYKTIYGEQKLIYADWIASGRLYR
                     PIEEKMLHLFGPMVANTHTESSFTGKFMTEAYHYALKEIKRHVNAGPEDVIVTSGFGM
                     TSSINKFQRILGLKNKSFVFKNPKERPVVFVTHMEHHSNQTSWYETIAEVVVVEPDKD
                     LLVSPQKLRETLERYNDRPMKIGSFSACSNVTGIKTPYHELAKIMHGAGGLCFVDFAA
                     SAPYVKIDMHPADPMERLDAIFFSPHKFLGGPGASGVLIFNSNLYKNRVPDQPGGGTV
                     DWTNPWGEYKYIDDIERREDGGTPGFLQAIRSALAIRLKEQMGVENIEKREEELVHLA
                     FERLSKIKGLHILADNCLNRIGAISFYIDNLHYNLIVTLLSERYGIQMRGGCVCAGTY
                     GHFLLNVSKEQSHRITDLINKGDLSEKPGWVRWSLHPTTTNAEVEYIANALAEIVAYG
                     KEWQNEYSYCKHTNEFCNKNPEAAMLWKNVEEHFQF"
     gene            complement(25666..26130)
                     /locus_tag="PLW22_01425"
     CDS             complement(25666..26130)
                     /locus_tag="PLW22_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOU73392.1"
                     /translation="MSTANGGSLNFPAYQFRVKQENGNRYIFDVIRKKFIALTPEEWV
                     RQHLLSYLITDLNLPVGLIGVEVSFSFNGVNHRADVVVYNRKGEPILLAECKAPNVEI
                     TNEVVDQICRYNYALNAEYLLVTNGLKHLILWLSEGKGWITLDGFPELRNKI"
     gene            26342..27112
                     /locus_tag="PLW22_01430"
     CDS             26342..27112
                     /locus_tag="PLW22_01430"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOU73393.1"
                     /translation="MKTKEEIVENWLPRYTGQALEDFGSHILLTNFRGYLEFFANYFN
                     VPIVNPNANMPCATANGITMIDFGMGSPNAATIMDLLSAVKPKAVLFLGKCGGLKKKN
                     NVGDFILPIAAIRHEGTSNDYMPPEVPALPAFTLQRVVSSTIRNHNRDYWTGTVVTTN
                     RRVWEYDDNFKEYLRRTRAMAIDMETATIFTVGFYNEIPTGALLLVTDQPMISEGVKT
                     AESDQKVNSLFVEEQIKIGIESLTRLINNSESVKHLRF"
     gene            27122..28168
                     /gene="holA"
                     /locus_tag="PLW22_01435"
     CDS             27122..28168
                     /gene="holA"
                     /locus_tag="PLW22_01435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOU73394.1"
                     /translation="MAKQSQTVETFQNILQELKNKIYKPIYFLTGDEPYFIDKIAEYI
                     EDNVLSSSEKAFNQTVVYGRDISAADIINAARRFPMMSNYQVVIVKEAQNVRNLSDLE
                     IYLKTPLNSTILVVCYKIASDGKKSSDKFSGVVKLAKQKGIYFESKKFYDYQIPDWIV
                     GYLKDKGIKIDPLAANLLTEFVGNDLSRLAHELDKLAIILPADSKQIQATDIERNIGI
                     SRDFNRFELTKALGEMNFARAMRIADHFARNPNANPFVLTITAIYQYFIKILKYHFLA
                     DKSKASVAKELGVNEFFVAEYERAAKRYSPSRCVSIIHWLSEYDMRSKGVNNGSTDHG
                     ELLRELIYLIVFGK"
     gene            28165..28779
                     /locus_tag="PLW22_01440"
     CDS             28165..28779
                     /locus_tag="PLW22_01440"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU73395.1"
                     /translation="MTYIIIAITAIVSFAAFGNSILFQQLLLNPYIVFKRNEWYRLIS
                     HAFVHANFLHLFVNMIVLLSFGRYVESILKQLQSIGSIGMHQVHFLILYLGAAIVSSL
                     TTLRKHKNDPYYQSVGASGAVSAIVFFSIFFSPLEKLYLMAIIPIPGIVFAIAYLFYS
                     NYMSRKGGDNINHDAHFVGAVFGFIYPILIDPKLFFFFLKQLGL"
     gene            28790..29218
                     /locus_tag="PLW22_01445"
     CDS             28790..29218
                     /locus_tag="PLW22_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU73396.1"
                     /translation="MQAEAQITIHPDGLDASVLQLVAIVSYMNGNWLWVRNKNRDTWE
                     LPGGHIEPGELPVEAANRELFEETGAIRYSIKQLFDYTVRLDGAVSYGRMFYAEITEL
                     ETLPNSEIGEVKAFKSIPENLTYKRVQPRLFEMAIEYLKG"
     gene            29234..30097
                     /locus_tag="PLW22_01450"
     CDS             29234..30097
                     /locus_tag="PLW22_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOU73397.1"
                     /translation="MTQNAYIWYNGEFHRADSMVLAVSNRSFRYGDGLFETIHCYGTE
                     PYHLELHYERLTKGLQILEIEKPSSISKVKLGEVITKLLNKNRHFGSTRVRVTVFRNG
                     EGLYTPESSDASIIVESQPLDFKFYAYNQQGLIVDVYPDIKKSVNILSTVKSCNALLY
                     VKAGLYRKRNLLDECIILNEFGRVAETISSNVFVVKRGEIFTPGLTEGCIPGIMRRVV
                     INLARSLGYKVFDDVALNTKVFSTCDEFFITNAISGIRWVLGLGSNRYFNTVSRHLSI
                     ELNKATFKDLV"
     gene            complement(30089..30688)
                     /locus_tag="PLW22_01455"
     CDS             complement(30089..30688)
                     /locus_tag="PLW22_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOU73398.1"
                     /translation="MAKGKEIDINLFDIKFKQIPPKSGRVLIAQPSLNDPFFKRTVIY
                     LVEHDANGCMGFIVNRKLNISLSDILVDFPDVDVNVSVGGPVSPETINFLHTFGDLIP
                     NSYPLGNGIYMNGDINALKSLAIAGKVNAKNLRIFIGYSGWGPKQLENEIKEKSWVVA
                     NFAPADVMKGVYDNWYFAVQQLGEKFKVWTIYPENPSLN"
     gene            30879..32372
                     /gene="nhaC"
                     /locus_tag="PLW22_01460"
     CDS             30879..32372
                     /gene="nhaC"
                     /locus_tag="PLW22_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOU73399.1"
                     /translation="MIKQPKLIHGIIPIAFLILFLTLNVIYFGDETLSGANQIALLLA
                     STIGSAIAIHLGYKWSYIRQIIVKTIGSAMPSILILMLIGALAGTWMISGVVPTLIYY
                     GLKILHPKIFLFATVVITALVSLATGSSWSTVATMGVALLGIGNALGFNHAIIAGAIV
                     SGAYFGDKMSPLSDTTNLAPAMAGTDLFTHVKYMTITTIPSLAITLIIFLVIGLRFEP
                     QGVVDVQTTMNTLTSTFNISPWLLLVPIFLIVVIVKKTPPLPSIMFGVLLGGVFAIIF
                     QPHIVEQVSGVTDNYPEAGYIAVMKSMFGKVTINSGDAQMNELLTTRGMAGMMNTIWL
                     IITAMIFGGVMESAGLLVKITETIIRFAKSTASLVASVVTSCLFFNVTASDQYISIVV
                     PGRMFAKTFRKRGYKPELLSRTLEDSGTVTSVLIPWNTCGATQAAVLGVPTFSYAPYA
                     FFNIISPFMSVFIATIGFKIRRFTPEEMQEIEREDENSPVELKFEQE"
     gene            complement(32409..34820)
                     /locus_tag="PLW22_01465"
     CDS             complement(32409..34820)
                     /locus_tag="PLW22_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU73400.1"
                     /translation="MSTTKIKFSKEEILNDYYIAHLSRNISLMGRKEVLTGKAKFGIF
                     GDGKELAQIALAKTFKEGDWRSGYYRDQTFMMAAGLFTAKEFFAQLYGQTDIELNPGN
                     AGRTFNNHFATRSLNPDGTWKDLTKQKNSTPDISPTAGQMPRLLGLAWASKLFRNNKE
                     LWRFEQFSRKGNEVAFGTIGDASTSEGHFWETLNAAAVLQVPMVVAVWDDGYGISVPR
                     DYQTAKGNISELVKGFEKGESDVNGIYIYRVKGWDYPALIETFTQGVERSRTEQVPVL
                     FHVTEITQPQGHSTSGSHERYKSPERLQWEKDYDCNARFRNWIVTEGYATETELNEIE
                     AKAQADAKAAREKAWAEFTNPIKKERDELIKIIDNRSCFCKREHIDKVGEITEQLKAI
                     PNPIRKDNISAAKRILRHVCSDCSIREKLQADLTAWIERNMNDGYDRYSSHLYCETER
                     AAWKVKGIPPVFKDDSPLVAGREILRDNWDAQFQKNPLLVIFGEDVGGIGGVNQSYEG
                     LQKKYGEIRISDTGIRENTILGQGLGLALRGMRPVAEIQYFDYLLYAIQTMSDDLATI
                     RWRTKSGQMAPLIISTRGHRLEGVWHAGSPLSMVINSIRGVYVCVPRNMTQAAGFYNT
                     LLEADDPALVIEPLNGYRLKEKRPENIGEYKIPLGVPEILREGSDVTLVTYGSCVRIA
                     QEAVEQLWEFGISVELIDVQTLLPFDLPNLILDSIKKTNRIVFFDEDVPGGATAYMMQ
                     KVLEERAGYQYLDSPPKTITAREHRPAYTTDGDYFSNPNAEDVFDGIYELMHEVNPKR
                     YPKLF"
     gene            35001..35246
                     /locus_tag="PLW22_01470"
     CDS             35001..35246
                     /locus_tag="PLW22_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73401.1"
                     /translation="MLTLKLILVAATILGIALLGMAVRIIFHKSHKFPETSAGHNKEL
                     RKRGVTCPRHEEIKCWGKNGKNTDCATCYEHAREVVN"
     gene            35258..35440
                     /locus_tag="PLW22_01475"
     CDS             35258..35440
                     /locus_tag="PLW22_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73402.1"
                     /translation="MALLKLIGVVAFLLGIVIIGLSLKTIIKSRKSGCNDSDIGYSCG
                     CGGGTNCSTNAASKGI"
     gene            complement(35441..36436)
                     /locus_tag="PLW22_01480"
     CDS             complement(35441..36436)
                     /locus_tag="PLW22_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOU73403.1"
                     /translation="MRYLTVFTLAVIILVSCSPKKEYLKIEGFTQGTTYSITYYDSLI
                     RDFSVQIDSILGRIDHSMSIYNDSSIISLLNSNISDSIDDMLKEVIVSSFEIYHQSDG
                     AFDITVGPVVRAIGFGKDKTQGIDSAKVKQLLPLIGMNKIHLDGNTLKKDDSRIQIDV
                     NAIAQGYTVDVIGKYLESNGIKHYLVEVGGEITARGKNSKGTDWVVGIDKPVESALPG
                     ENIQAKISISNGYGLATSGNYRKFIEVNGQKYAHTINPKTGFPELNSLLSATVVAPSA
                     TKADALATAFMVKGVEWSKNFVVKSNDIDAYLVYSDSLGNYLVWMNEGMKKRIIP"
     gene            36527..39517
                     /locus_tag="PLW22_01485"
     CDS             36527..39517
                     /locus_tag="PLW22_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU73404.1"
                     /translation="MLNLLGDQAYLNSYQLDIYDTSFDKLMQKRIYKVLLICSSYDAF
                     LLEEDGRIDEQIFNEYVSLNLRYPPVFLQAHSAREVLRILQEEHIDLIISMLNVGEMD
                     TFNLAKMLKQRYPSIPIVVLTPFSREVSLRLQNEDLSAIDYVFCWLGNADLLLAIIKL
                     IEDKMNAEHDILEIGVQTIILVEDSVRYYSSFLPNLYKIIFRQARMFMTEALNEHHRM
                     LRMRGRPKILMATTYDEAIELYNKYKNNILGVISDISYKVNNKRDHVAKAGLQLCRHI
                     KADDQNIPFLLQSSELSNKIYADELCVGFLHKYSKTFSLDLRDYIVNNFGFGDFVFRD
                     PDTLAEIARASDLRQLQKLILTIPDRSLVYHSSRNDISKWLNARAIFSIAQLFKPMRL
                     KDFRSIDDARNFIYYAISAYRLSKGRSIIAKFDRETFDEYKFFSRIGDGSIGGKARGL
                     AFVNQVIRNYNLFNKFENTIITIPRTVVLSTDIFDEFMESNNLYSIALQDLDDEVILE
                     AFLKAKLPERIKIDLRKVISVFQRPLAVRSSSKLEDSHYQPFAGIYSTYMVPHSDNPD
                     QMLQHLAKAVKAVYASVFYKASKAYMNATQNVIDEEKMGVIIQEICGNQYGNMYLPTL
                     SGVARSINFYPIGSEKTEDGIVSVGFGLGKLIVDGGVALRFSPKYPKKILQLSSVEMA
                     LRQTQKNFYALNVDPNSFEPKVDDSSNLLNLDIREASSIPDFRLITSTYDMHSGVLRD
                     GYYDEGLKLVTFAPILKHDTFPLAKILSELLEIGEREMNNHIEIEFAANLNPPSGMPR
                     LFNFLQIRPIVDSDQTELVEWNEMDINKAILYSKSALGHGKVKDIYDFVYIKPESFNP
                     AMTKEIAHELDELNKRFIDLQRNYVLVGPGRWGSSDPWLGVPIKWSQISQARVIVEAG
                     LENFRVDPSQGTHFFQNLTSFRVAYLTVNPYLHDGSYDIQFLNSIPAWFETKYIRCIR
                     FEQPLVIQVDAKTNQGVILKPENG"
     gene            39687..41024
                     /gene="gdhA"
                     /locus_tag="PLW22_01490"
     CDS             39687..41024
                     /gene="gdhA"
                     /locus_tag="PLW22_01490"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOU73405.1"
                     /translation="MNVNKLMAELEAKHPGEVEYLQAVREVLESIEEVYNQNPQFESA
                     KIIERMVEPDRIFTFKVPWMDDEGNIHVNLGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNALTTLPMGGAKGGSDFNPKGRSDAEIMRFCQSFMLELWKHIGPETDV
                     PAGDIGVGGREIGYLYGMYKKLAGENTGVLTGKGINWGGSLIRPQATGFGATYFAQEM
                     LATKGMSFAGKRVAISGFGNVAWGAALKVTELGGKVVTISGPDGYILDEAGISGEKID
                     YMLELRASNQDIVEPFAKKFPGAKFFAGKRPWEVKVDVAMPCATQNELGKADAEALVK
                     NGVVCVAEGANMPCTPEAIEVFQHNKILFAPGKAVNAGGVATSGLEMTQNSMKLNWPS
                     EEVDARLHQIMKSIHEACVKHGTEKDGYVNYVKGANIAGFLKVANAMLDQGLI"
     gene            41093..41506
                     /locus_tag="PLW22_01495"
     CDS             41093..41506
                     /locus_tag="PLW22_01495"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU73406.1"
                     /translation="MSTTSTIIYKGDLRTEATHTRSGVTITTDAPEDNQGKGECFSPT
                     DLLATSLGCCMVTIMGIAARTHGFNIDGTKIDVQKVMGTNPRRVVEVIIDLYFPHNNY
                     TAKERKLLEASAKECPVAQSLHPDLKQTIRFHFPE"
     gene            complement(41536..>42162)
                     /locus_tag="PLW22_01500"
     CDS             complement(41536..>42162)
                     /locus_tag="PLW22_01500"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOU73407.1"
                     /translation="HSLGEFSALVAAGALSFEDGLKLVSARAMAMQKACELQPSTMAA
                     VLGLDDAKVEEICASINEVVVAANYNCPGQLVISGSVKGVDMACEKLKEAGAKRALKL
                     AVSGAFHSPLMEPARQELEAAINATPFKKPVCPIYQNATAKPTSSPEEIKKNLVAQLT
                     APVRWTQTVQNMIADGATEFVELGPGSVLQGLIKKVNKDISAESKQSL"
BASE COUNT        11885 a         8836 c         9139 g        12302 t
ORIGIN      
        1 tggcggtggg cttagtaagc gataccacat gagtcacgcc gcaggcgtgg cgaattgtat
       61 cgcgtggcag ttccacccac ggggtaccca tcgggtcggg cttttcagcc ttgattttct
      121 ttggttcttt cttgtatcaa gacaagaaag aacgtgaaaa tcactctttg cgatgttttt
      181 cctttgcgat gcaaagtttc agtgttgctc tttggaaaat gtattacgtg catcgctata
      241 catgtgggaa taatcatagt gttacaaata ttacgcccct gcggggctgg aaacggtgtt
      301 cgttgctcgt gaaactattg tcatgttgag cgttagcgaa acatctcatt acacctaatg
      361 tggcagccaa ctttgctagc tctatccgtc atattcttgt tgggcttaac tgttcacttt
      421 ccctctaact tcttctaact cagtcatatt cttttgtttc cgaatcattc cgaatcattc
      481 cgaatcattc cgaatcattc cgaatcattc cgactaattc ctctaaattc atgttacttg
      541 ggataaactt aacattaact taacataccg tttaaacctt aacatatagt ttacatagac
      601 gtaactatct ttgcacaaaa tttttagcaa tgagaaatat tgttttccta tcggctttaa
      661 tggttttgca acattttgtt tatgcgcaag aggtaaacga ctcaataatt taccatcaaa
      721 atcaaaggat attgcagttt ttggaaagca gtaagatttc aacccaaatt tttcttggct
      781 acaggtatta tgaggaaggc aatggaaact ataacgagtt tgtcgtaaaa aggggatata
      841 tcacctttga taagtcattg aacaagtatc tatccgggcg catagctccc gacctaacaa
      901 ttgacaagga aggcgatggt gaaggcgacc ttgaaatgcg cttaaagtat tgttatgttg
      961 agcttaaaga cgagggcagt tatggggtat tcacaagccc taaactatta attggagagg
     1021 ttcctacccc ttttatcgat tttgaggaaa aggttaacgc gtacagggtt gttgctagcc
     1081 agtatctcga tgacatgaaa atcctcacat cggccgattt cggggttacc acatcggttt
     1141 tactgggtgg aaagattgat gagggttacc aaaaaactgt cagcaatgct tatcccggca
     1201 aatttgggtc aattgctgtg ggggttttta acggaggtag ttaccatgct cttgaataca
     1261 ataacaataa aacttttcag tggaggttaa gtgtacggcc aatgcctgag cgtttacctg
     1321 gtttacagtt ttcctatgca ggagcacatg gtaagggtaa tacagcactc taccccgatt
     1381 gggatacaca tacgggtttc ttgagctatg aatctcgcag acttgttctt acagggcagc
     1441 tattccgttc gctaggaaac catgagggta cactggccga ttctttgggc aactcataca
     1501 cagctaaggg tttgaatgct ttcctagaag taaagttctt gaaaaaccgc ttcagcttgt
     1561 ttggtgctta cggtgttatg cgttatactg actatgccgg aacagatatt gagaaaactc
     1621 gcgagattat tggattagcc tatcatatct acaaaaagaa taagtttgtt attgatttaa
     1681 accgaagtat ttccaaagga aaatgtaagg gggttattga atttatgttt gagttggctt
     1741 tgtagtaatt actttccgtt tcaacatagt aagtataaaa aaaggttggc aaagcgccaa
     1801 cctttttttg gctaaaggtg aggttagaat agtccgctga taactccatt gttacccaca
     1861 tcaatacgct cggctgcagg ttcatcgggt aatccgggca tacgcattat ctgtccggta
     1921 attggaatga taaatcctgc gccagcagca acctcaattt ccctaacagt aaccacaaaa
     1981 tccttaggtc tgccaagcag gtcggggtta tcggagagcg atttttgagt ctttgccata
     2041 caaattggca gcttgtcgag ccccagtgag tatacctttt ttaaatcctc ctttgcttga
     2101 gcggtgtaat ctacagcttc agcaccgtat atcttcttgg caagggtttc gatttttgct
     2161 tcgggtgtcc agttccagtc gtaaagggtt ttatgctggt atgggcagtt catggcaatc
     2221 ttgttaacaa ttttagccaa ctctatggca ccctcgccac ctttacccca cacatcaacc
     2281 tctgcaacct gaacatccat ttccctgcaa acgcttttta tgtattcaac ttcctcatca
     2341 gtgtcagttg taaacctatt tatggctacc acggggcaga tgccaaaaag tttcatgttt
     2401 tccacatgct tttgcaggtt gccaataccc ttttttagag cctctacatc gggttgtgaa
     2461 acctccttaa gtggctttcc gccatggtat ttgagagccc ttattgtggc tacaagcaca
     2521 actgcacttg gttttaaccc tgcatactgg cacttgatat caatgaattt ttcggctccc
     2581 agatcgaaac caaaaccagc ctcggtaacc acgaactcac tgagcgagag acccattttt
     2641 gtggctataa ccgagttggt accctgggct atattggcga atggtccgcc atgaataatg
     2701 gcaggggtat tctcaatggt ttgcactagg ttaggcatta tggcatcgcg taaaagcgca
     2761 gccatggcac cctcggcatg taaatcgcga gcatagatgg gctttttatc gtaggtatag
     2821 cccacaaaga ttttccctaa cctctgcttc aggtcttcca tgttttcggc taggcagagt
     2881 attgccataa cctccgaagc agctgttata tcaaatccgg tttcgcgggg taccccatgg
     2941 gttgtaccac ccagcccaac aatgatgtgg cgcaacgatc tgtcgttcat gtccatcact
     3001 cgcttccagt gcacagtacg gggatcgatg ccaagggaac gctttttgct ttgtaaatta
     3061 ttgtctatta gcgcagcaag aaggttgtgt gccttttcaa tggcgttgaa atcgccagta
     3121 aaatgaaggt tgatatcttc catgggaaga acctgtgagt atccaccacc ggttgctcct
     3181 ccttttatac cgaacacagg gcctagcgag ggttcacgga gaaccactgt ggtttgttta
     3241 ccaattcggt taaggccttg cgccaagcca attgatactg ttgttttccc ctctccggca
     3301 ggtgttggtg aaatggccga taccagtatc aacttgtttt gctgatactt ttccttgtcg
     3361 atgtagctca agggaatttt ggctttatac ttaccgtaag gtattaaatc gtcaggattg
     3421 atgcccagaa ttccggctat ctcggttatg ggcttcattt tagcttcgtg tgcaatttca
     3481 atatcgcttt tcatagtaaa aatgatttag gttattcagg gaaagtgtta ttaacttatg
     3541 tagatttcat catacaagtt ttaccaatta tgaataacaa gttacagcaa aatttgttta
     3601 tgggcaactt tgcttacggt ttatatcttg ccagggtaag gatttcctga taatccttta
     3661 taagcattag cctctgaaga tcgatatttt tgtttcgctc cataaagcgc tcaacaattc
     3721 ggtagcctat ccaaactgca gcccttccgg gcgattcctg tggaaacccc gatgtaaatg
     3781 gagcatcgtt tatcatcttg ttgatgtcaa cagtattggt ggtaaagagc agcttatttt
     3841 caataaggtg agtccacatg gcagactcgt tatttaggca ccacttgtac tgctgtggtg
     3901 taaaaccaaa aattagggag tcgggttgat atgggagcat ccgcttggtt aggtacatga
     3961 tttgcccttc gtaaatcatg ttactcagta ggttgttgat tgaatcgtgg aaagggaaag
     4021 cgctgtacag ccaggcccgc ataacatccg atggaacttt accggggtgc atcttacgtt
     4081 gctggtaaac aggtatgcca agcagggaat actgtatggt atttctaccc aaatagcggt
     4141 cgagccccac ggccaccaaa ctatcatcta ttgccaatga taggttataa cctgaaatat
     4201 aggtgtaaac ctttggtagt ggtttgcttg ggaaaaagaa agagtacctc gcgaacgcat
     4261 cgctaatctc cttttcaaat ggagcattgg ctgggaatac actctcaacc attctgttgg
     4321 cgttttgtac catggtatcg gtgaggaata accctaagta cctgtaaaaa tcgggggaat
     4381 cgttcttccc aattcgtata atgccctcag taaacagttt tgtgaaactt gtgtgctttt
     4441 gataaacaat agggaaggct ttttcaatgc tgtcaggatt aatactaaaa agctctttct
     4501 cgaacctggt aatggaaaca ggttcaacat ctctaggggg ttgaattgag tcgaatcggt
     4561 tttccttaca gccctgaatg gccaaaacgg taatggctgc tactatccat ttgttcctca
     4621 tggtgtttaa gtttttaaaa gcagaacaaa gatagtaaat acatggataa aggataaagg
     4681 ataaaggata aaggataaag gataaagaat gtctagtcct gaaataagtg acccgtcctg
     4741 aaaaatgtct acataaaatt ttgtgaataa aaaacgattt gataaaactt ctttaccatt
     4801 tgatggctat ctttgaatgg attatagtca attgagttag gactgtaaag catttggggt
     4861 ttaatgctta atttttaagc tatgattgat gttgctcagg tatttggttt gctcggttca
     4921 ataactgcgg caagcttatt ctttccacaa gtttatactt catggaaaac caaaaagacc
     4981 cacgatttgg cttggtctgg aattgctatt ggtctgctga acggagtttt ttggattgtt
     5041 tatggcctaa tccgtttaga tcccttcata tacgtaacaa acacaatact attcattggc
     5101 gcattcctgt tgcttattct taagaaacgt tacggttaat ctaggctcag tgaccgcagt
     5161 ttattcacta cattttctat tactttccta ttgtattgag aatttgtggg tttaccccat
     5221 cggttaataa cttgttagta accgatgttt gaatgtgaaa aaacactatg ggatgcagct
     5281 tccgtttcgc gaattgaaat tctacctttg ctttgcaaaa acaaataaaa atggccggag
     5341 agagaaaaag tactggaaaa aagccatatt cacctgctaa accaatggta tcggagctag
     5401 gtaaggttcc cccgcaagcg gttgatattg aacaggcagt gcttggcgct ataatggttg
     5461 aaaaggatgg tgtaattatt gcaagcgaga tccttaagcc cgaatgtttc tataaggaag
     5521 cacaccaaat tatctttaaa gccattcagg atttatcggc aaggcttgaa ccaatcgata
     5581 ttttcacggt aaccgaggag ttgcgtaaac agtccaaact cgatgaggtt ggcggggcag
     5641 cgtacatcac tgagcttagc tccaaggtgg gatctgctgc acaccttgag tatcactcaa
     5701 agattattgc acaaaagtat atccagcggg aacttattcg cgttgccacc gatattgaac
     5761 gacgttcatt tgaggatgat attgatgttg atgagctgct ggactactcc gaaatggaac
     5821 tctttaaggt ggcagagggt aatattaaaa gcgatactca acccattagc aacattgtag
     5881 ttaaagcatt caagcagata gaggaggctg gtaagcgcga ggatggtctt agcggagttc
     5941 catctggtta tactgacctg gataggttaa cctcgggttg gcaaaaatcg gacttaatta
     6001 ttgtggcagc gcgcccttca atgggtaaaa cagcattcgt actatccatg gcgcgcaata
     6061 tggccgtgga gtacgacgtt cctgtggcat tcttctccct tgaaatgtca aacattcagc
     6121 tggtaaaccg tttgatcatt gctgaatcgg gcatcccctc ggaaaaaata cgcaacggaa
     6181 ggctatcgaa tgacgaatgg actcagctaa ccgttaaaac caagcagcta gccgatgcca
     6241 agatttttat tgatgatacc cccgcgctct ccatatttga gctaagggct aagtgtagga
     6301 ggcttaaggc gcagcacaac ataggaatag tgattattga ctacctacag ctgatgaccg
     6361 gcccaaccga taccaagggt aaccgagagc aggaggttag caccatttcc cggtcgctaa
     6421 aggcaattgc aaaggaactc gatattccta tcattgccct ttcgcagcta aatcgttcag
     6481 ttgaaacccg cgggggtaat aagcgtccgc agctctcaga cctcagggag tctggtgcca
     6541 ttgagcagga tgccgacgtg gttattttta ttcaccgccc tgagtactat ggctttgatg
     6601 ttgatgacag cggcaattca acccttggca ttgctgaaat aattgttgcc aagcaccgta
     6661 acggtgcagt tgatgatgtt cgactgcgat tccagaagga aatggctaaa ttctccgatt
     6721 tggacgatat gactattatt cccaatacta ttactcctac ctatacatcc tctgcccccg
     6781 ttacctttag ctcaaagatg aacaacgacc ctgttgacga ttttacgttt aatcccggta
     6841 agggaattga taataccaat ataccttttt agggttaaag gattttttta atctcaacta
     6901 gctccctcac ctcaaaggta actttggcct ggtgagggtt gcttttgggg ttgaaataaa
     6961 tttgatccat accgaacttc tttgctccca gtatatctat ctcaaggtcg tcgcctatca
     7021 taatactctc gcccttacgg gcgttagcct ttgataatgc atacccaaag gctcgcttat
     7081 cgggcttgtg gaatcctgag tgttccgatg ttacaaccca ctcaaagtac ttatcaatgt
     7141 tgcttctttg cagtttaggt agctgaacct ctttaaaccc gtttgagata acgtgtagct
     7201 tatacctatt ggccaggtaa tcgagagttt cacgagtgcc agggattagc gcagttttta
     7261 agggcataat ggttaagtac tcatcgccaa tggtttcggc aaggggtttg ctttcaatac
     7321 catactcagc caaggtaatc tcaaaccgct tgtagcgcag aacttcctta gtcaactcac
     7381 ctttaaggta cttatcccac aggtaatggt tgtttcggtt aaagcagtca atgaaagtat
     7441 caggattagg aaaagccaag tccagcttat ggttagcgta gatttcagca agcgcatcgc
     7501 gcatgttctg ctcaaagtcc catagcgttc tgtctaaatc aaaaaaaata tgcttgtaag
     7561 cgttgtgctt gaagaaaaag ttgagtatca tggtgtagta ttaacggcac aaaaatagtt
     7621 atagtaatta atgcctgcaa acctataact tcagaatagg agtcaagcaa ggttaccctt
     7681 acttttttac ttcattgagt ttgatttcgt cttatttaaa tgataaatgt taacttggga
     7741 aaaagtctga atgaattatt catgttttgt tcagaaagcc tatgcctaag cattaaaact
     7801 taaatgtata gatttctaca aaaaacctta acttgtaaat ggaaatgggc aatattaaaa
     7861 tctgttattt tattaacagt taatttttgc ctttgtatct ttgagctgaa attttaaacc
     7921 gtactagaat gagcacatca aatccaacca taaaagtgct gatggtagat gcagcaagtg
     7981 gcttttacaa ggtgaaccgt ttccaagtgg gcgatttttt tgggccgatt gatttgggaa
     8041 tacatctatc gcataagcat aacagcttaa acataggggt tggactttta gctggttcaa
     8101 tttttcctgg ttctaaccgc ctgattgtaa acggaatatc accgtgttgg catggttttt
     8161 acatatcgtc gatggggggt gcaggtttgg tattcgataa ccttggaata aatatgtttt
     8221 ccattgtagg taaggctgca gcaccgtcaa tcctttatct taaccgtatt catggtgagg
     8281 aaattgaggt agaacttctc cctgtccggc cacgaaccat ttgggccgag ggtcgtggtg
     8341 gggtttactc catgatgcag tatgcactca accagtttgg tagccgttat gagaatgaac
     8401 cccgaatact tgctaccggg ccagcatcga tgtacaccga ttttggcgct attgcctcag
     8461 ctccaatccg taagaatgag attacctatg ttgacacctg ggctggccgt ggaggctttg
     8521 gtaccaaaat ggtgcagcag cacaacattg ttgctattat ttacggtgga acatatatcg
     8581 atgaggactt ccgcgaccgt accgttgccg atagctggtt tgaggatcgc taccagaaac
     8641 gccttgctgc caaggatatg gaggctacca ccaagtatcg cttcgatcct aagtttgaaa
     8701 ccggtggaac ctttggcgta aactacgcca ccatgaaggg cgatattatg gccttcaact
     8761 atcgaacaat ctacatgacg gaggaggaac gccttaaaat ccataacgat ttcatactaa
     8821 accactacct taagcagttt aacgaggaaa ccattaaaac caagcagcaa cgtacctgtg
     8881 gtgaaccttg tggggctgta tgtaaaaaaa tgcacgggga gttcaaaaaa gactatgagc
     8941 cctaccaaac catggggcct ctttgcggaa tattcgatca gcgagctgct gagaagttaa
     9001 accaccatgc cgatatgctt ggtttcgatg ccatttcggt gggtggtgtg ttatcgtggc
     9061 ttatggactg catggccgat ggtcttgtag agcctcaaga acttggcgtg gccgatattc
     9121 ccgtatttac acccaatggt ttccgtgttg tggaggactc aatgcataac gctaacattg
     9181 gaattgcttt actcgaccag atggtgaatc ccaatggagt ttttcacttg cgcgaggggg
     9241 cacgtaagtt tgcccgtcat cttgcccggc gtaagggaac atctgtactc gataagttta
     9301 tatacatagg ttttgcgcgc tccggctgga tggtacccaa ccagtactgg acacccggag
     9361 tactatcgcc catggcaatt atgggtaagt actacatgta ctacggaaag gattttgttc
     9421 ctcctcgtca gttgggaagg gaaaacgcta agcgcatgct aaacgagctt gtaatggata
     9481 acctgggaat gtgtcgcttc cacagggcat gggctgagga tattctccct gatgtaattg
     9541 agcaacttta cgggttaaag gaaaagtttc aacgatcgat acttcttact gcaagccgaa
     9601 taaacagccg taacgcatcc atattctggg aaagcgaaag gaatatcgat tttgtttact
     9661 cctttctaag gaaaaagaag gaggttgatg gtgcaagcaa tcctgaactc gacagatgga
     9721 taaagtattt tgaggaggac aagcgaagtg ctgcacttga tttctggtac gaaatccata
     9781 agggcgtaca tgaatcgctt cttgaatttc attaatagtt atatttgacg aaaaaaatga
     9841 agatcattcg taggatactt tttttcattg cgctattact agttgctata gctgcattcc
     9901 tttactgggg tacttatgag gaaggcgtaa tggcagggaa aataattcgt attagccaga
     9961 aaggtttgat attcaaaacc tatgaggcca agttaggcct tgagtcgttt ggcgcattaa
    10021 ggggaaccag ccccattgct gaaaccttcg atttttcggt tgaacccagt aatcatgaac
    10081 ttgtaaagga gctagagcag gtagctctaa gtggtgaaag ggtaaacata cattacaaga
    10141 aacgctatat cagggttccc tggcggggca gcaccaagta ctttgcaaca agagtggaac
    10201 gtttacagag gtaaaataaa aaaggcagcc aaatggctgc ctttctactt ggtatacttg
    10261 aaagaatcct agtaatccct tctgccaccg cgaccgccac cacggctatc gcgaccctcg
    10321 cggctgtaac cgccacgacg ttcaccgtaa ccaccgccac gacgctcacg gttgaatcca
    10381 ccaccgttgc ggggcttgtc ggttttgggt tttgcttctg aaacgctgat taccttacca
    10441 tcgtattcag cgccggtaag ttcctcaatt gcagtacggg cctcttcatc attggccatc
    10501 tcaacaaagc cgaatcctct cgaacgccct gtttccctgt cggtaattac tttggccgag
    10561 ttaatctggc catactcttc gaaaagttgc cttaggtcgt catcgttaat gcggtaactc
    10621 agacctgaca cgtaaatgtt cataaactaa aattagattg tgattgataa ataaattgtt
    10681 tttttgattg aggccttgct taactgatgt tttgtcggga atcggctaga tgcggcaaaa
    10741 tattctgtgt tgaagcgttt ctcaattcgc tgcaaaggta gtgcaattaa tttaacaatc
    10801 accattttcg gaaaattatt tttacaaggg aaaggcaagg aagcttaagt cagatattag
    10861 gaacccaaaa gtgtattctt gaaactcaat ccaaatcaaa accatgtgca ctaacatttt
    10921 taaaaaaaag tgggtacctg aatgggcacc cactgattaa gagaagtata attgattttt
    10981 acttggttgc ttcttccaac ctacgcataa tggagaagat gaaaattgcc gagagtaagc
    11041 agcaaacaat aaagattgtc cagagctgcc atagctcaat tctttcccag aagaagctgc
    11101 ctacaaaaag caacttgttt ccaatggcag tagcaagtag ccaaccaccc tgcattaatc
    11161 cctggaagcg ttgaggagca acctttgata caaacgacaa tcccattggg ctgaggaata
    11221 actcagcaat ggttagaatc aggtagctgc taattagcca gtaaggcgaa accctgcccc
    11281 attcggttag cccttgcgcc tcaatggttt tgggagctat taggtgaatt gaactcaaca
    11341 gcacaaccac aaatccaatt gcagcaatta tcatccctat acctattttc ttaggggttg
    11401 agggctcaat attccgtttg ttcatccagg caaaagcacc cataacaggg aatgtaagca
    11461 ctactatgaa caatgggtta aacgattgga aaacctcggg ggcaattggg ttggaagccc
    11521 caaatttgat agcctggtag taagttaaat aaccaaggcc aagaaacata attagtccaa
    11581 ttactctctc acgaatctgt ttacgggcaa acattaacac taaacctgca atagtgccaa
    11641 taaacgaaag catcgaccat aagttgaaga agatattagt gaaaggtcca acctccttaa
    11701 cggtatagtc gcgggcaaag agggtcaagg ttaaaccgtt ctggtggaac gacatccaga
    11761 aaaagattac aaccacaaat acgagtagaa gcgaggtaac ctttggaagt tcttccttgg
    11821 ttgacatcat gaacatcatg gccacaaagc caaagaatag accaatggcc aatcctaaag
    11881 caatttcggc tccgagtaac cagaaaatcg ctccagtcaa taatacaagg gcaatgggta
    11941 caattacgtt tagaaggttc tttttggtaa atagcgatcc cgcagctggt gtagtgctaa
    12001 cttttttctc cttattgggt aattttttgt tgaaaattac atatacaagt agcgagatca
    12061 ccattgcaac tgcagcaaca ccaaaagccc agttataccc gcgcgaaaag gcatcaatgt
    12121 atttatgagc aaaatcggtg agctgttcag taccggtaat agtggcaccc gaaaccttgc
    12181 tggcaaggct ttcgaacttt gcgagcgact caggatcggt taccttaccg tttaggaatg
    12241 catggcaaag cgagggtagg ctggcatcgt gctcaaagcc ttggctgcga aggaaccagt
    12301 tacggatacc ggttgcaaca aatggggcaa agaatgcacc tacatttata cccatataaa
    12361 aaatcatgaa accagtatcg cgaacctttg agtattcagg gttgtcgtac atttggccaa
    12421 ctaccgcctg taggttacct ttgaataacc cattcccaaa tgcaatgatg aaaagcccaa
    12481 gcaatgtcat ggttaggctc atgccaggca tggcaatcat tgcgtagccc ccaaacataa
    12541 tgattatccc cagcagaatt actgttttat acctattggt tgcatcggct attaatccac
    12601 caataagggc taacgcataa atggcaaagt aaaaccagct gtagtaacca ccggcttcct
    12661 cggccgatag gccatacttg gcttggagaa ataacaccag gatggccatc atggtgtaga
    12721 aaccgaaacg ctctcccatg ttggagaaga acgctaccag tagtccttta ggatgatttt
    12781 tcaacatata aattatggtt ttggtttatc ttcaattttt caagattgac aaaaatagta
    12841 tttaatggtt tatttccaaa tccacaaatt atgttgtata atgtttttgt taagcttatt
    12901 ttacctaaat gttaaaagcg ataaccattt attggtttta atttgtaaat acaattaacc
    12961 gaagtgccat gatttaacag attcagacgg gattagagga atgagtagga atgagcggaa
    13021 tatgatggag ttagaggaag ttagaggggg ttagaactag tgaaaagtga aaagtgaaaa
    13081 gttaaaagaa gaattgagga tatgaggggg ttggagagct ttatgttcgt gaaccgtgaa
    13141 ccgtgagccg tgaaccggaa gaattaagaa tatgacggag atatagggag ttatagggaa
    13201 gttgaactgt taaatctaaa taccatggat taacataatc aaccgggtta aaaaaaggga
    13261 gagggataca tttatagagg agattcatca atggattttt tacaattgac ctgttcagga
    13321 taaacaatcg ggaaaaatta gtaacttagc actatagcct gaatcaatga aagtattcaa
    13381 aacaagccag atcaaaagca tcgatgctta taccatagaa aacgaaccca ttgagtcaat
    13441 tgatttgatg gaacgggctg caacagccat tttcaactgg tttcgaagca aataccataa
    13501 gaatcgaagg attaaaatct tttgtggccc cggtaataac gggggcgatg ggcttgcatt
    13561 ggctcgtttg ctaattaata cgggctttaa cgcgcactgt tactacttaa aagccgattc
    13621 ttactcagcc gactttttaa caaatcttga tcgtctaaag gagttaacgc cggaattatt
    13681 tttagttaaa catcaatccg attttcctga aattcatacc gatgatctgg tagttgatgc
    13741 cctatacggt tcggggctta gtcgatccat tgaaggggtt gctgctcaac taattgagca
    13801 tatcaactca tcgggggcta aggttatcgc tattgatatt ccctcgggtc tttttggcga
    13861 ggccaatcca gtacccaaca ataaccctgt ggtaaagtcc gactactgtc ttagcttaca
    13921 gtttcccaag ctggcttttt ttatgccaga aaatgagcaa tttttaaagc gctgggtagt
    13981 tctacccatt ggtctgcata cggaggctat tgaaaatacg gctacaccgt accattatac
    14041 cacggcaagt agtatcaaaa ccgatttttt ccagttgtca acctttgccc ataaagggac
    14101 ttacgggcat gctctagtta ttgcaggttc caacgggatg atgggagcgg cttgcctatg
    14161 tgctaaatcg gttgtgagga gtggggtagg attagtaaca atgcatattc ccaaggtagg
    14221 ctataccatt gcccaggtaa atgttcctga agccttaact agcatcgaca gctgtgaaac
    14281 tcacattacc caattccctg agttgtatcg atatcaagcg gtttgcgttg gccctggcat
    14341 tgcacaaagt accgaaaccg atcaaatgct cgtcaagttg attaaaacct gccaattacc
    14401 tttggtggta gatgccgatg gactaaactt actttcaagg aatattgatg tactcaatag
    14461 tgttaacaat gcattgattt taacaccaca tcccggagag ttcgatcgac tttttgggaa
    14521 atcgcagtgt agctgggaga ggcttgaacg tgcaagagaa atggcacaaa aatacggggt
    14581 gataattgtt ctaaaaggag cctacacaag ggtagtattg cccagtggca atgttcattt
    14641 caactccaca ggaaatccgg ggatggcaac cggaggaagc ggcgatgttc ttagtggaat
    14701 aattaccgga ttgcttgctc gtgggcttcc cccggccgat gctgccatta ccggtgttta
    14761 tatccatggc cttgcaggcg acttggccgc cgacgaattt ggacaaatta gcttgtgtgc
    14821 ttcggacatt gtgaactata tttgtaaagc attccaacac ataaccaaca atgatgagta
    14881 aaaatctttt cttagcagtt ctatcccttg tattattggg ctgtaatagt aaaactattc
    14941 aagttgaacc ggctggtaaa acaattgact ataaaaaagt tgcagtttac tattcgttac
    15001 cccgaacagt ggtttacgta aaggttgtta gctctcaaag cgaatatttc cccggcccat
    15061 atgccgatta tgccaaaaag tttttgggta tcgacggggt aagtaatacc gaaaaggttg
    15121 agtatctaat tgagaaaata gatgtagaaa ccaccgctga accggatccc aaagcagtct
    15181 ttgctattta cccatcggga aagggttatg gtaactttat aaatctttct aatgaagggt
    15241 taatattacc tgttaaccaa acccctgaaa gtagagccat tgctaaaaac aaagaagtaa
    15301 gcagtaaagg agaggtaatt tataaggatt tatcggttaa cccttttgtt gccgaggaat
    15361 caactacatt ttatggtaga accctgcgcg acagtgttta cgtaagggtg cccatacagc
    15421 gttcaatgac cattgaacgc aaccttgagg aaaaagctaa ggaggcagcc gattttattt
    15481 ttagcatccg aaaaaaaagg ctcgattttc ttacccctga tatcgaccat ccattcagtg
    15541 gcgatgcact taagttgatt ttgaaggaac ttcaaaggct ggaggacgaa tatctggcac
    15601 tatttgtagg taaaaggttt actcacacat tcactcaaac ctatagcttc attccttcga
    15661 acaccgaagg cgagagcgct atccttttcc gtttttccga aagcaaagga ttgctttcat
    15721 caaccgacct ttccggacgc ccaatactca ttgagtttga tcaacctgaa tatcctgaga
    15781 atattgatgc tactaagaat agcctggaaa ccattgcttt aaaagccaaa atcgacaggt
    15841 tttactacag gatacccgtt gcagttaaca tgagagttat tgatggcaag cagctacttg
    15901 ttgaaaagcg tttaagcata ttccagtatg gactgctagc ccaactgcct gttaattttc
    15961 caggtaagtg attaaggatt tgctactgta tttagtttac ttacaaactt tgcccgaaaa
    16021 tcttcccatt tctgggtttt gtaagcatcg ctttcaatgt aagccattat tggttctagc
    16081 tgttcaggag taaggtatcc ggcaatgtag gtaattaggt tattgttctc gtccataaac
    16141 actattgagg gatacgataa ccttccttgt agaatagcta tagcaaactc atgcggattg
    16201 cgttgcccct gcccctgccc cttattcttg tataccttac ctttatatgt tatatctttt
    16261 gaaccttcgg cattgaactt tacggcatga aacttttggg tgaggtacga ggctattaca
    16321 gggttggtaa aagtattctc atccatcttc ttacaccagc cacaccagtc ggtataaaca
    16381 tcgatgaata tttttcgggg cgttttttcg tttacctcaa aagccctatc tatttcaatc
    16441 cagttgattt ttgtttcttg tgctatggaa accgaagcgg atatgccaag catccaaata
    16501 gttaaaaccc agattctgtt tttcatggtt ttgattttgg ttaacggtaa atgagttcaa
    16561 aaataatgcc ttaatatcac tttgcatcac catctttata ccagcctgag taaaagaggt
    16621 agttgcgggc aatacgctca ttggtttcgc ggctaagttc aggggcaacc tttttgacaa
    16681 aacgggctgg aactcctgca taaacacatg cgggttcaac aacagtgttc tccaatacca
    16741 gtgcgttggc agcaactatt gctccttcgc caactttagc gttgtccata acagtacaac
    16801 ccattcctag caaggctccc cggcttacct gggcgccatg aagtattaca ccatgtccaa
    16861 tcgatacatc atcgccaacc tcgatggttg ttttctggta ggtagtgtga agcaccgcat
    16921 tatcctggat attaaccctg ttacctattc taatggagtt aacatcgcca cgaagcacag
    16981 cgccgtacca aatactgcaa tcatcgccaa tttcaacatc acctattatc actgcagtct
    17041 cagcaataaa gcagttctta ccaattttag gtgtaattcc cctcaagggt ttgattattg
    17101 ccataccatt ttgtaattta ctgcaaattt actacttact aacagttatc gtgccggttg
    17161 agttcaaaaa gtttgtgaac attagagttt atggcgatct gaacgatttt ttgccacaag
    17221 gcaaaaagca taaggatcag cgtgtaggtt tttttgggat gcccactgta aaggacttgg
    17281 ttgaatcgat aggggttccg catgttgagg tttttctgat tttagccgat agcctaccgg
    17341 ttaaatttga ttaccgtcct gctaacggca cactaatttc gtgttaccca aaatttactt
    17401 cattgccatt atcggcaatt gctttaagac ctgattttga aaagcccccc aggtttattc
    17461 ttgatgttca tttaggtcga ctggccgcat acctccgctt ttgcggaatc gacacactat
    17521 acgataagtc gtttaatgac aatcagatac ttgatattgc acaagccgat ggccgaatta
    17581 ttctgacccg cgataagggt atcctgcgta acggccaagc ccaatttgga tattttctca
    17641 gaaacaccaa tcctcagctg cagcttgccg aggtggttca ttactttgac cttgctcact
    17701 accttgatcc gttttcaagg tgctcactgt gcaatggtga ggtagaacag gttgataaat
    17761 ccatggttaa aaacttagtt ccttcaacta cctaccagat ttacagtgag ttttttcagt
    17821 gttccagatg tgggcaggtt tactgggaag gacctcactt tatcaggatt tccaagctgc
    17881 ttagtaggta ttcagagtaa ttattagctt atttttatgg ttaaggtaaa aatgaaaaac
    17941 atgccgtaaa atatgtttta aaacgattgc tgtgtataaa aaagcctaat tttgtgtcat
    18001 aaataaataa gtagtaatca atataacatt ctggatatca gatttatata attcaggatg
    18061 tttaaaaact aaacagcatc tttatggata aacaaaaaag tgtccaagag ctggacgagg
    18121 ttgttgttcg attttcaggt gattccggcg atggtatgca gcttaccgga accctgtttg
    18181 ccgatgcatc ggcacttttt ggtaacgatg taagcacttt ccccgattac ccatcggaaa
    18241 tacgtgcccc acagggcacc attggtggcg tatcgggttt tcaggtgcac tttggaaacc
    18301 gaaaagttaa tacccctggc gatttttgcg atgtgttaat tgcaatgaac cctgcagccc
    18361 taaaagccaa cgccaaatgg gtaaaacgtg gcggtatgat tatagttgat gccgaccagt
    18421 ttaatgaaaa ggggtaccag cgtgccggct acaaaacttt cgatgcagta tcggagttaa
    18481 acctgcagga ttacaaggtg atttttgctc ccattacaaa ccttaccaag gaaagcctta
    18541 aggatagcgg attggacaat aagagcatca tccgtagtaa gaacatgttt gccttgggta
    18601 tggtttacta catattcgat aggccattaa actttaccga gaaattcttt gaaaagaaat
    18661 tttccaaaaa gcccgaagta gttgaagcaa acaagaaagt tttacgcgat gggtttaact
    18721 acgccgcaaa tattcaggcc attgctagca cctataaagt aaagcaggca actactttac
    18781 ccaagggtaa ataccgtaac attaatggaa acacggctac cgcatggggt ttaatagcag
    18841 ctgccgaaaa agcgggctta cccctgtttt gtggttcata ccccattact cccgccactg
    18901 aaattttaat agagctagcc aaacgtaagg atttaggcgt taaaaccctt caagctgagg
    18961 atgaaattgc tggaatatgt actgcaattg gcgctgcata tgccgggaac tttgcagtca
    19021 ccactacatc gggccctggg ctttcactga aatctgaagc aatggggctt gccgtaatga
    19081 cggaacttcc tttagttata gttgatgttc aacgcggtgg cccttcaacc ggtttgccaa
    19141 ccaaaaccga gcaatccgac cttatgcaag ttctctgggg acgtaatggc gaggcgccag
    19201 tggtagtaat tgccgccagt accccaagca actgcttcga ttacgctttt atggcaggta
    19261 aaattgccat ggagcatatg actcctgtag tacttttaac cgatggttat attgccaacg
    19321 gctctgagcc atggcgcatt ccaagcatga aggattaccc tgaaataaaa ccacctattg
    19381 ttccagaggg aaccgaaggg tatatgccct atgctcgcga tgaaaaacgc ttagcgcgtg
    19441 gttgggcttt ccccggtaaa aaaggtattg cccatagagt ggggggcttg gaaaaggatt
    19501 tcctaaaagg taccgtttca cacgacccaa ccaaccacca acgaatggtc gatatccgtg
    19561 ctgcaaaggt tcagcgtgtt caggagttca tccctgagca gcaagtgatt ggcgaccccg
    19621 aaggcgacct gcttgttatt ggttggggcg gaacattcgg tcatctgttc gatgcagttc
    19681 aggaaatgcg tgcaaacgga aaaaaagttt cactgtgcca tttcaactac ataaacccca
    19741 tgcccaaaaa tgttgccgac atttttggtc gtttcaaaaa gcttgtggta tgcgagttaa
    19801 acatggggca gtttgctaac cacttacgaa tgaattatca gcaattcaac tacctacagt
    19861 ataacaaggt tcagggtcta ccattcaccg tggtggagct ggttgaaaag tttaaccaaa
    19921 ttttggaggg taaatagcca tggaagaaca atatatgaat tacaccccac aggattttaa
    19981 gagcgatcag gaagtgcgtt ggtgtccggg ttgtggcgac cacgccattc tgaatgccgt
    20041 acaaaaagcc ttacccgagg tatcaaaaca gttaaagtat aaccttgaac gctacgtatt
    20101 tgtctctggt attggttgtt ccagccgttt cccctactac gttaatacct ttggtttcca
    20161 tggcatacat ggtcgtgcat cggccatagc aaccggagta aaggtggcaa acccaacgct
    20221 gagcgtttgg caggttactg gcgatggcga tgcccttgcc attggaggta accactttat
    20281 ccacgcagta aggcgtaaca tcgatattaa catcttactt ttcaacaacc agatttacgg
    20341 gttaaccaaa ggtcagtact caccaacctc gccacttgga gcaattacca aaacttcacc
    20401 atttggtacg gttgagcatc ctttccgccc aggtgaactg gttattggcg ctcagggaaa
    20461 gttttttgcc cgttcgctcg atgttgatgt taagcttacc gctgagatta tggttgaagc
    20521 tgctaagcac gatggcacgt cggttgttga aattcttcaa aactgcgtca tttttaacga
    20581 tggggcttac gattacataa ccgaccgtga acatcgcgac gatagagtga ttgttctccg
    20641 tcatggcgaa aaaatggtat tcggaaaaaa cagggataaa ggactaattc tcagcggact
    20701 gagccttaag gttgtaacca ttggccaggg tggctttacc atggacgata tacttgtgca
    20761 cgatgcctat aacccaaatc ctggtgttca catgatgctt gccaacatgg agtaccccga
    20821 ttaccctgta gcccttggtg taatccgtaa cgtaaaagac tctacctacg atgataacgt
    20881 acgcgaccaa gtgcttgaag tgatgaaaac cgccaagatt aagtcagttg acgatttact
    20941 caacagtggc gatacctggg aagtataaaa ccttattatc tctttttaat aaattaaggc
    21001 tgccaacttc aggcagcctt ttttcaatat tttttttcac tattacgctg aacattagtt
    21061 tatttagtta ataaatgcta aatttgctta tttgatgatt taaaagttta atgcaattgg
    21121 ttgcaaagta tggtaaagca aaagtttatt aaggcattaa tcgcactgca atttattctt
    21181 agtttctcag ttagcgaggt taaggctcag gatagtttca agcaaatctt tagcgatgca
    21241 gagtattact tcttccttca ggacttcaag gaggcacttc ccctttacca gagcctatac
    21301 caacaagatt ctaccaatgc caatatcctt tacaagctag gtatgtgtta cctgaacata
    21361 cccggtttga aacagaatgc tataccttac ctagaaaaag catcaaagaa tgtaaaccct
    21421 aaataccgtg aagggtatta ccgcgagact gccgcgccca ttcaaaccta tttttacctg
    21481 ggacaagcct acatggtaaa ttttaagttt gatgatgccc tgctggcttt tcagaagttc
    21541 aaggataata ttgatgttaa agatgtttac aacctcgact atgttaacca gcagataaaa
    21601 gcatgcgagg tggctcacaa tttcatttcc aggccaattg tgatgaaaac cgaacatatc
    21661 gacctgtttc ccgataggaa taagaattgc aattacccag taatatcagg cgataggcaa
    21721 accatggtgt ttacggttaa ggaaaaattc tatactgcag tatactattc ccggtggatt
    21781 gatggcaagt ggagttcacc aaggaatatt acacttgacc ttagggttga gggcgaactt
    21841 tatactaccg cccttaacta caccggcgat tacttgattc tttttgtcaa tgaagtaacc
    21901 tccggcaacc tatactactc aacgctggta ggcgataagt ggcaacccgt taaaaagtta
    21961 ccagcaccaa tcaattcaaa ggattgggag acctttgcgt cgctatcggt cgatggcaaa
    22021 cagatgtatt ttgtttcgaa ccggaaaggt ggctatggcg gaaccgatat ttacatgtcc
    22081 agtttgcagc ccgatggcaa atggagcaac ccaataaacc ttggtccaca ggtcaatacc
    22141 ccatacaacg aggagtcgcc aattgtatgc cccgacggac gaacgcttta ctttgcctcg
    22201 caggggcata acagcatggg aggattcgat attttttaca gccgaaagat tgatggtaat
    22261 tcctggtcta tgcccataaa tcttgggtat ccatttaata cacctgacga tgagttttac
    22321 ttttaccccc tcgattcact ttctggcgta atgccgatgg ccatttccaa tcaatcaacc
    22381 ttttacgaac tttacaaggt aaacatctac cccagtatat cacgtaagat agaactcttt
    22441 ggcaaggtta atctctctga caatgccgat attaaatccg acagtatcgc tattttagtt
    22501 aaagatactg caaacaacct tattgccatg gctttaccct taagcgatgg aacttacagc
    22561 gttgcaatta agcctggcag atactccctt gaggctacca gccaatttta cgtaatgaac
    22621 ccactgcagc ttcacatacc caccacctat aaccaggaaa agtatctatt agatatcaac
    22681 cttgatgcta agccaataac caaagaggag gtgattcggt tcaactatgt acttttcgat
    22741 tttgacagct acgagctgaa acgcgatgca cagtttgaac ttgaaaaggt ttacaagctc
    22801 atgactgatt atcccgactt gtatatcgag gtgattggcc atactgacag caagggctca
    22861 cccatgtaca atctcatgct ttcggcccgt agggcaaatg ccgtagctga gtacctggtt
    22921 aataagggaa ttgacgaaaa acgatttgta gtaaggggta tgggctcact ggtaagcttt
    22981 gccgctaata ccaaccccga cggttccgat aaccccaatg gccgtaagct aaaccgacga
    23041 gccagcatta gggtatttaa cccaaacaag aatcttaaaa ttgagtttgt tgatgttccc
    23101 gaacacctca aacctcaaac ccagaactat accataatgc ttgcacctat cgacgataca
    23161 atttctcctg atctaattaa agccattgag aagaagttta atatcaacct tcgtgagttt
    23221 gtaattggat cacgacgatt ggtttgtatg aatgtttaca agtcaaaggc cgacgccatt
    23281 gagcatctca ataccattat tgatatgggt gcatcaaggg ccgtgctggt aaatgaggtt
    23341 gaacttcaac gccttgtggc tgctttgcag aaaaatctta taacaaaaca aagcgttttt
    23401 accattcttg ttgccacaag cgagataccc ttaacgctcg actttttccg ggggctatat
    23461 gtaaccgagg aggttggtaa cgacgggtta taccgttact actttggagc cttcaatgag
    23521 aaagctaagg ctaccgagat gctggaaaag gtaaactcaa tgggattccc caatgcaatt
    23581 ctggtaaaac ttgagaaaag gtaatggagg gtaatgtttt agaaaacagc gttgtaaagc
    23641 ttcgtgcacc tgaaccagag gatgtcgatt tgctttacag ctgggaaaac aacatggaaa
    23701 tatggaaggt cagtaatacg cttacgcctt tctcaaaata cctgctcaaa aagtatatag
    23761 agacatcgca tttagacata tgggaatcca agcagctacg gctgattatt gaggctaagg
    23821 atcagaactc gcttatgaat gttccggttg gccttgttga tttgttcgat tttgatccct
    23881 accatttgcg ggcgggcgtt ggtgttttga tagccaaaaa cgaagaccgc agaaaaggtt
    23941 atgctactga ggctttaaac gtgctaatcc gatatgtatt tgaggtacta cagctaaacc
    24001 agctctatgc aaatgtgtcg gcaagtaatg ttattagctt gcgcttgttt gagaaccttg
    24061 gttttactaa ggttggtgta aaaaaggatt ggttaaaaac attgaatgga tgggaggacg
    24121 aggttttact tcagctatta aatcctaaac ctcgcctacc caaaagttaa aactggcgat
    24181 aattttagcc ttactggtta aaattgaaag tgctcctcaa catttttcca aagcattgct
    24241 gcttcagggt tcttgttgca gaactcattg gtatgcttgc agtacgagta ttcgttttgc
    24301 cattccttac cgtatgcaac aatttccgct aatgcgttgg caatgtactc tacttcggca
    24361 ttggttgttg ttggatgtaa cgaccatcga acccatccgg gtttttcgga caggtcgcct
    24421 ttattgatta aatcagtaat ccgatgcgat tgctccttac ttacattaag taggaaatgt
    24481 ccgtatgtgc ctgcgcacac gcaccctcca cgcatttgta tgccatagcg ctcgctaagt
    24541 agggttacaa taaggttgta gtgcaagtta tcaatataga acgaaattgc tccaattcga
    24601 tttaggcagt tatcggctaa aatgtgtaac cctttaattt tactcaaccg ttcaaaggca
    24661 aggtgaacta gctcctcttc gcgtttttcg atattctcaa cacccatttg ctccttcaat
    24721 cgaatggcta atgcactacg gattgcttgc aaaaagcctg gagtacctcc atcctcgcgt
    24781 cgttcaatat catcaatata tttatactca ccccatgggt ttgtccagtc aaccgtgcca
    24841 cctccgggtt gatcgggcac acggtttttg taaaggtttg agttgaagat gagaacgcct
    24901 gaggcaccag ggccacccag aaacttatgg ggcgaaaaga aaatggcatc taaacgctcc
    24961 atgggatcgg caggatgcat atcaattttt acgtatggtg ccgatgctgc aaaatctaca
    25021 aagcataaac ccccagcccc atgcatgatt ttagcaagct catggtaggg ggtttttatt
    25081 ccggttacat tgctgcatgc gctaaatgaa ccaattttca ttggtctatc attgtatcgc
    25141 tccagtgttt cgcggagttt ctggggcgat accaaaagat ccttatcggg ttcaaccact
    25201 acaacctcag caatggtttc gtaccacgat gtttggttgg agtgatgctc catgtgtgta
    25261 acaaaaacca cgggcctttc cttggggttt ttgaatacaa agcttttgtt cttaaggcca
    25321 agtattcttt gaaatttgtt aattgacgat gtcataccaa accctgatgt aacaattaca
    25381 tcctcggggc cagcgttaac atgtcgttta atctctttca gggcatagtg ataagcctct
    25441 gtcatgaact ttccggtaaa gctactctcg gtatgagtat tggcaaccat tggcccaaaa
    25501 agatggagca ttttttcctc tataggcctg tatagcctgc cactggcaat ccagtcggca
    25561 tatattagtt tctgttcacc ataaatggtt ttataggtag catcgatacc tacaatgttt
    25621 ttacggaatt gctcaaaata cttttcaagg ttactcatat cttagttata ttttattcct
    25681 tagctcaggg aagccatcta gggttatcca tccttttccc tcagaaagcc acaaaattaa
    25741 atgctttaac ccgttagtta ccaacaaata ttcggcattc agcgcatagt tatacctgca
    25801 tatctgatca acaacctcgt tggtaatttc cacgttaggc gctttacatt ccgcaagtag
    25861 tattggctcg ccttttcggt tatagactac aacatcggct ctatggttta cgccattgaa
    25921 tgagaaactt acctcaacac ctatcaatcc tactggtaag tttaaatcgg ttatcaggta
    25981 gctaagtaaa tgctgacgaa cccattcttc aggggtaagt gcaataaatt tttttcggat
    26041 tacatcaaaa atgtaacggt taccattttc ctgttttacc cggaactggt aggcaggaaa
    26101 gttaagcgaa cctccgttag ctgtgctcat gcagcaaaat taaaaatttt taaatcatcc
    26161 taggtcttgc taacgttttc gccagccaaa acaagctggt aattattagc ctttactaga
    26221 aaatttcacc aaaatagcac atcacttttg ttaagtatgt ttaaaaacac tattttaaga
    26281 ttgagcgaat agccgtatct ttgccgaaat atacttgttg ataaaatata aatgtgttgg
    26341 tatgaagaca aaagaagaaa ttgttgagaa ctggttacca aggtataccg ggcaagcact
    26401 tgaggatttt ggttcccata ttcttttaac caactttagg gggtaccttg aattctttgc
    26461 taattacttt aatgtcccca ttgttaatcc aaatgccaac atgccctgtg ccactgcaaa
    26521 tggtataacc atgatcgatt ttggcatggg tagtcccaat gctgcaacta taatggattt
    26581 gttaagcgcc gtaaaaccca aagcggtact tttccttgga aaatgtggag gcctgaaaaa
    26641 gaagaacaac gtaggcgatt ttatactacc cattgcagct ataaggcacg aaggaacctc
    26701 aaacgactat atgccccctg aggttcccgc tttgccagca tttacgctac agcgtgtagt
    26761 ttcctcaacc attcgtaacc acaaccgtga ttactggaca ggtactgtag ttacaacaaa
    26821 ccgaagggtt tgggagtacg acgataattt caaggagtac cttcgccgta cccgtgcaat
    26881 ggcaattgat atggaaaccg caaccatttt tacggttggt ttctataatg aaattcctac
    26941 cggggcactt ctgctggtta ccgaccaacc catgatttcg gaaggggtta aaaccgctga
    27001 aagcgatcag aaggtaaatt cactctttgt tgaggagcag ataaagattg gtatagaatc
    27061 attaacccgt ttgataaaca acagcgaatc ggtaaagcac ctacgttttt aagtttgata
    27121 tatggccaag cagagtcaga cagttgaaac gtttcaaaat attcttcagg agctgaagaa
    27181 taagatatat aaacccattt actttctgac cggcgatgaa ccatacttta tcgacaagat
    27241 tgctgagtac attgaggaca atgtgctcag cagttccgag aaagcattca accagaccgt
    27301 ggtttatggt cgggatattt ctgctgctga tattatcaac gcggcacgtc gctttccaat
    27361 gatgtccaac taccaggtag taattgttaa ggaagcacag aacgtgcgta acctatccga
    27421 tttggagatc taccttaaaa ctccgttgaa ctcaacaata ctggttgttt gttacaaaat
    27481 agccagcgat ggcaagaaat cgtccgataa gttctcaggg gttgtaaagc ttgcaaagca
    27541 aaaggggatt tactttgaat caaaaaagtt ttacgactac caaattcccg attggattgt
    27601 tggatacctt aaggataaag gtattaaaat tgacccactg gcagcaaacc tactaactga
    27661 gtttgttggt aacgatttga gccgcttggc ccatgaactt gataagctag caatcatact
    27721 tcctgctgat agcaaacaga tacaggccac agatattgag cgaaacattg gtataagccg
    27781 cgattttaac cgttttgaac taaccaaggc gcttggcgag atgaactttg cccgtgccat
    27841 gcgcattgcc gaccactttg cacggaatcc caatgccaat ccttttgtgt tgaccattac
    27901 tgctatttac cagtatttca tcaaaatatt aaagtatcac tttttagctg acaaatcaaa
    27961 agcatcagtg gccaaggagt tgggtgtaaa cgagtttttt gttgctgagt acgaaagggc
    28021 agcaaaacga tacagcccat cgcgctgtgt aagtattatc cactggctca gcgagtacga
    28081 tatgcggtca aagggtgtta ataacggttc aaccgaccat ggcgaactgc tgcgggaact
    28141 aatttacctt atagtatttg ggaaatgacc tatataatca ttgctattac cgctattgtt
    28201 tcctttgctg catttggaaa cagcatccta tttcagcagt tactgcttaa cccatacata
    28261 gtatttaagc ggaatgagtg gtataggttg atttcacatg cttttgttca tgccaatttt
    28321 ttacacctat ttgtaaatat gatagtgttg ctatcgtttg gcaggtatgt ggaatcaata
    28381 ttaaagcaac tacaatctat tggtagcatt ggtatgcacc aggttcactt tctcattctc
    28441 taccttggtg cagcaatagt ttcatcgtta accacgctga gaaagcataa gaacgaccca
    28501 tactaccaga gcgtaggtgc atctggtgct gtctcggcta ttgtgttttt cagcattttc
    28561 tttagtcctc tggaaaagct ttaccttatg gctattatac caattcctgg aattgtgttt
    28621 gccattgctt acctttttta ctcaaactac atgagccgta agggaggcga taatataaat
    28681 cacgatgcac actttgtagg tgcagtattc ggattcattt acccaatcct tattgaccca
    28741 aagctgtttt ttttcttttt aaagcagttg gggctatagt ttgataccca tgcaagccga
    28801 ggcgcaaatc actatacacc ctgatggttt agatgcaagt gttttgcaac tggtagctat
    28861 tgttagttac atgaatggga attggctatg ggtgcgtaat aaaaaccgtg atacctggga
    28921 actaccagga ggccatatcg aaccgggcga attgcccgtt gaggctgcaa atagggaact
    28981 ttttgaggaa acaggagcaa ttcgatactc cataaagcag ctatttgatt atacagtgcg
    29041 gctcgatggg gccgtatcct atgggcgaat gttttatgcc gaaataactg aacttgaaac
    29101 tttacccaat agtgaaattg gggaagtaaa agcatttaag agtatccctg agaatttaac
    29161 ctacaaaagg gtacaacccc gtctttttga aatggcaatt gaatacctaa aaggatagct
    29221 atctttgtat gtgatgaccc aaaatgctta catatggtat aatggtgagt tccatcgtgc
    29281 cgatagtatg gtattggccg tttcaaaccg gtctttccgt tacggcgatg gtctgtttga
    29341 aacaattcat tgttacggaa ctgagcctta ccatctggaa ttacattatg aaagattgac
    29401 caaaggatta caaattctgg agattgaaaa accctcttcc atatcaaaag ttaaacttgg
    29461 ggaggtaatc accaagttac tcaataagaa ccgccatttt gggagcaccc gagtaagagt
    29521 tacagttttt agaaatgggg aggggcttta cacacccgaa agtagcgatg catcaattat
    29581 tgtggagagt cagcccctag actttaagtt ttacgcttac aaccaacaag gtttaatagt
    29641 agatgtttac cctgatatta aaaaatcggt aaatattcta tcaacagtaa aaagctgtaa
    29701 cgccttactt tacgtgaaag cgggacttta caggaaaaga aatttactcg atgagtgcat
    29761 tattctcaac gagtttgggc gggtagctga gactatttca tcgaatgtct ttgttgttaa
    29821 gagaggcgag atatttacac cagggcttac ggaggggtgt attccgggaa ttatgcgcag
    29881 ggttgtaata aaccttgccc gcagtttagg gtataaagtt tttgatgatg tagccctaaa
    29941 caccaaggtt ttcagcacat gcgatgagtt ctttattaca aacgcaatta gtggaatccg
    30001 ttgggttctt ggacttggga gcaatcgcta ctttaacact gtgtccaggc acctcagcat
    30061 tgagctaaat aaagcaacct ttaaggattt agtttagcga tggattttca ggatatatag
    30121 tccaaacctt aaacttttcg cccagttgct gaacggcaaa gtaccagtta tcgtaaaccc
    30181 ctttcattac atcggctggc gcaaaattgg ccactaccca tgacttttcc tttatctcat
    30241 tctccagttg ctttggcccc cagccagagt aaccaataaa aatccttaaa ttcttggcgt
    30301 ttaccttacc ggcaatggca agcgatttta atgcgttgat atcgccattc atgtaaattc
    30361 catttcccag ggggtatgaa tttggtatta gatcgccaaa ggtgtgcaga aaattaatgg
    30421 tttcagggct aaccggtccg ccaaccgaca cgttcacatc aacatccgga aaatcgacca
    30481 atatatcgct aaggctgatg tttagcttgc ggtttacaat aaatcccatg cagccatttg
    30541 catcgtgttc aaccaggtag attactgtcc gcttaaaaaa cggatcgttc agggatggct
    30601 gggcaattag cactctgccg gatttaggtg gaatttgttt gaacttaata tcaaacaggt
    30661 tgatatcgat ttcttttcct tttgccataa ccacctcctt cctgatttgt ttcactcttc
    30721 aactatccaa aaatatgcca aatttttctt ttgaattgct tttccataat aattttcctg
    30781 aacgattttt aaatgaacgc atcctttgta tgttaatgct cggggacgat ggagttcata
    30841 tcaggattat tgctattttc gaaaaatttt aattgggtat gataaagcaa ccaaaactca
    30901 ttcatggcat tattcccatt gcatttctga ttctttttct cactttgaat gtgatttact
    30961 ttggcgatga aacgctatcg ggagctaacc aaatagcatt gctgcttgct tcaactatag
    31021 gttccgctat tgctattcat ttgggctata agtggagtta cattaggcaa atcattgtaa
    31081 agaccatagg atcggccatg ccatcaatac taatactaat gttgattggt gcactggctg
    31141 gtacatggat gattagcggc gttgttccaa cactaatata ttacggttta aaaatccttc
    31201 atcccaaaat atttctgttt gctacggttg ttattacagc attagtttca cttgcaacgg
    31261 ggagttcttg gtcaacagtt gccaccatgg gggtggcctt gcttggaatt ggcaatgcgc
    31321 taggctttaa tcacgccata attgctggtg caatagtatc gggagcctat tttggcgaca
    31381 aaatgtcacc cttgagcgat accactaacc tggcaccagc catggccgga acggacctgt
    31441 ttacgcatgt aaagtatatg accattacaa caattccttc attagcaatt acccttatca
    31501 tttttctggt tataggactt aggtttgaac cacaaggcgt tgttgatgtt caaaccacaa
    31561 tgaataccct aacctctacc ttcaacatct caccatggct tttgcttgta cccatctttt
    31621 tgattgtggt aattgttaag aaaacgcctc ctttaccatc aattatgttt ggtgttttgc
    31681 ttggtggtgt ttttgctata atttttcaac ctcacatagt tgagcaggta tcaggagtta
    31741 cagataacta cccggaggcc ggctatatag cagtgatgaa aagcatgttt gggaaagtaa
    31801 caatcaattc gggcgatgct caaatgaacg agttgcttac aacgcgcggc atggctggca
    31861 tgatgaatac catttggctt attataacag caatgatttt tggtggtgtt atggaatcgg
    31921 caggtttgct ggtgaagata actgaaacaa taattaggtt tgctaaatca actgcaagcc
    31981 tggtggcctc ggttgttact agctgcctat tcttcaacgt tacagcatcg gaccagtaca
    32041 tttccattgt agttcccggc cgaatgtttg ctaaaacatt ccggaaaagg gggtataaac
    32101 cagaactact aagcagaacg ctggaagatt ctggaacggt tacatcggtg cttatcccat
    32161 ggaatacttg tggtgcaacc caagccgctg tgcttggtgt tccaactttt agctatgcgc
    32221 cctatgcatt cttcaatatt attagcccct ttatgagtgt gtttattgct acaatcgggt
    32281 ttaagataag aagattcact cctgaggaaa tgcaggaaat agagcgggaa gacgagaact
    32341 cgccagtgga gttaaagttt gagcaggaat agatattaaa aaagcccggg aaactgggct
    32401 ttttttgctt agaatagttt agggtagcgt ttggggttaa cttcgtgcat caattcgtat
    32461 attccatcaa aaacatcctc ggcattggga ttcgagaagt agtcgccatc ggttgtatag
    32521 gctggacgat gctcacgtgc tgtaattgtt tttgggggtg aatcgaggta ttggtatcct
    32581 gccctctctt caagtacctt ttgcatcatg taggctgttg cacctccagg aacatcctca
    32641 tcaaagaata caattcggtt tgttttcttt attgaatcaa gaattaggtt aggtaaatcg
    32701 aaaggtaaga gtgtttgaac atctatcagt tcaacagaaa ttccaaactc ccaaagctgt
    32761 tcaacggctt cctgggctat acgaacgcag ctgccatagg taactagtgt aacatcggaa
    32821 ccctcgcgaa gaatctcggg aactcctaat gggatcttat actcaccaat attttcgggt
    32881 cgtttttcct ttaagcggta accattcaag ggttcaatta ccagtgctgg gtcatccgct
    32941 tcgagtagag tgttgtaaaa gccagcagcc tgagtcatat tgcgaggaac gcaaacgtaa
    33001 acacccctta ttgagttgat aaccatgctt aaaggtgagc cagcatgcca aaccccttcg
    33061 agcctatgac ctcgggttga aataataagg ggcgccattt ggccactttt tgttctccac
    33121 cgtatggtgg ccaggtcgtc gctcatggtt tggatcgcat acaggaggta gtcaaagtac
    33181 tgaatttcag ctacggggcg catacccctg agggctaggc caagcccttg tccaagtatg
    33241 gtattctctc taattcctgt atcgcttatg cgaatttccc catacttctt ttgtaatcct
    33301 tcgtacgatt ggtttacgcc tccaattcct ccaacatcct caccaaaaat aaccagaagg
    33361 ggatttttct gaaactgggc atcccaattg tcgcgaagta tttcacgtcc ggcaaccaaa
    33421 ggtgaatcat ctttgaaaac cggcggaatt cctttcactt tccatgcggc tcgttcggtc
    33481 tcgcagtaaa gatgcgagct gtacctgtcg taaccatcgt tcatgttgcg ttcaatccag
    33541 gcggttaagt cggcctgaag cttttcgcgt atactgcaat cggagcaaac atggcggaga
    33601 atgcgtttag ctgcgctaat attatccttg cggatagggt tgggtattgc cttaagctgt
    33661 tcagttattt caccaacctt atcaatgtgc tcgcgcttgc agaaacatga acggttatca
    33721 ataattttaa tcagctcatc acgttccttc ttgataggat tcgtaaactc agcccacgcc
    33781 ttttcgcgtg cagcttttgc atcggcttgg gctttagctt caatttcgtt gagttcggtt
    33841 tcggtggcgt agccttcagt aacaatccaa ttacggaaac gtgcattgca gtcgtaatcc
    33901 ttttcccatt gcaagcgctc aggcgatttg taccgctcat gcgaacccga tgtggaatgt
    33961 ccttggggtt gtgtaatttc cgttacatgg aaaagaacag ggacctgttc ggttcttgag
    34021 cgttcaactc cttgtgtaaa tgtttcaatc agagctggat agtcccaacc ttttacacgg
    34081 tatatatata ttccgtttac gtcggattcg cctttctcaa agccttttac aagttctgag
    34141 atatttccct tagctgtttg gtaatcccta ggtacagaga ttccatagcc atcgtcccag
    34201 actgcaacaa ccattggaac ttgaagcact gccgcagcat tgagggtttc ccaaaaatgc
    34261 ccttccgatg tgctggcatc gccaattgta ccgaatgcaa cctcgttccc tttacggcta
    34321 aattgctcaa atcgccaaag ttccttgttg ttcctgaaaa gttttgatgc ccaggctaac
    34381 ccaagtaatc ggggcatttg gccagctgta ggggatatgt caggtgtgct attcttttgc
    34441 tttgttaaat ccttccatgt gccatcggga tttaggctgc gggtggcaaa atgattgtta
    34501 aaggttctac ctgcattacc cgggttaagt tcaatatcgg tttgaccgta aagctgggca
    34561 aaaaactcct tagcggtaaa cagtcctgcc gccatcataa aggtttggtc gcggtagtag
    34621 cccgaacgcc aatcgccttc cttgaaggtt ttagcaaggg caatttgagc aagctcttta
    34681 ccatcgccaa aaattccaaa ttttgcttta ccggttaaca cctccttacg ccccataagg
    34741 cttatattgc ggcttaggtg ggcaatgtag tagtcgttta atatctcttc tttactaaat
    34801 ttaattttgg ttgtgctcat cgttcaattc tgttgagttt catcaggtaa acaaaaaagt
    34861 tgtgcaattg ttttgaaccc aaatcttaat gcccgacttt tgatttttta agctttttaa
    34921 taaaactttt tgtgtttcta tctgttagtt ttttgcgcga gcaggctaat aggtatactt
    34981 ttgcaaaaac ctttacttaa atgttaaccc taaaattaat tcttgttgca gccacaattc
    35041 ttggaatagc cttattgggg atggctgtta gaataatttt tcataagagc cataagtttc
    35101 cggaaacatc ggctggacat aataaggaat tgcgaaagcg tggtgttaca tgtcctcgtc
    35161 acgaggaaat taagtgctgg gggaaaaacg gcaaaaatac cgattgcgca acctgttacg
    35221 agcatgcccg cgaggttgta aactaaaact aaacattatg gcattgctta aacttattgg
    35281 cgtagttgca ttccttttag gaattgtaat tattgggtta tcgcttaaaa caattatcaa
    35341 gtcacgcaaa tccgggtgca acgattccga tattggttat tcctgtggtt gtggcggtgg
    35401 tacaaattgc agcactaacg ctgcaagtaa ggggatttaa ttaaggtata attctctttt
    35461 tcataccttc gttcatccaa acaaggtaat tcccaagcga atcggagtaa acaaggtaag
    35521 cgtcaatatc gttgctcttg acaacaaagt ttttgctcca ttcaacacct ttaaccatga
    35581 atgcggttgc cagagcatcg gccttggtag cactgggtgc aactacagtt gcactcaaca
    35641 agctgttcag ctcagggaat ccggttttgg gattgatagt gtgcgcatac ttttgtccgt
    35701 ttacctcgat aaattttcgg taattacctg aggttgctaa cccatagcca ttgctaattg
    35761 atatttttgc ttgaatgttc tcgcctggta gagcagattc tacaggctta tcgataccaa
    35821 caacccaatc ggttcccttc gaattcttac cgcgagcagt aatttcgcca cccacttcaa
    35881 caagataatg cttaatacca ttactctcca aatacttacc aattacatca accgtatagc
    35941 cctgcgctat ggcatttaca tcaatctgaa ttctgctatc atccttttta agggtattac
    36001 cgtcaaggtg tattttgttc atcccaatta aagggagtaa ctgttttacc ttagccgagt
    36061 caataccttg tgttttatcc ttgccaaagc ctatggctct aacaactggg cctacggtaa
    36121 tgtcaaaagc tccatcgctt tggtggtaaa tttcaaaaga gcttactatt acttccttta
    36181 gcatatcgtc aatactatcg gaaatattac tgttcaaaag actaattatg gaactgtcgt
    36241 tgtaaatgga cattgaatgg tcaattcttc caagtattga atctatttga actgaaaaat
    36301 cgcggataag ggagtcgtaa taggttatac tataggtagt cccttgagta aaaccttcaa
    36361 tttttaagta ttcttttttg ggtgagcagc ttactaaaat aatcactgcc agtgtaaata
    36421 cagttaggta tcgcatagct caatttttta caaatgtaac cctaaagcct aaactcctga
    36481 acaacctttt ggatactttt tttgtaaatt gccaaaaatt tgcgctatgt tgaatttgtt
    36541 aggtgatcag gcatatctta actcttacca gctcgatatt tacgatacgt cgttcgacaa
    36601 gctgatgcag aaacgcattt acaaggtttt acttatctgc agcagttacg atgcattcct
    36661 gcttgaagaa gacggaagga tagatgaaca aatctttaat gaatatgtct cgttaaatct
    36721 tcgatatcca ccggttttcc ttcaagccca tagtgcccgt gaggttctcc gtatactcca
    36781 ggaagagcat atcgatttaa tcatttccat gcttaacgtg ggtgagatgg atacctttaa
    36841 cctggcaaaa atgctaaagc agcgctatcc atccatccct attgttgtcc ttacaccctt
    36901 ctcgcgcgaa gtatcattgc gattacagaa cgaggattta agtgctatcg attacgtttt
    36961 ttgctggctc ggcaatgccg atcttctttt ggctatcatt aagcttattg aggataagat
    37021 gaatgccgag cacgacattc ttgaaattgg cgttcagact attattttgg tggaagattc
    37081 ggtaaggtat tactcctcgt ttttgccaaa cctttacaag ataatcttta ggcaagcccg
    37141 catgtttatg actgaggcgt taaatgagca tcacagaatg cttcgcatgc gtggaagacc
    37201 aaaaatcctg atggctacaa cctacgacga agcaatagaa ctttacaata agtataagaa
    37261 taatattctt ggggtaatat ccgatattag ttataaggta aacaacaagc gcgatcatgt
    37321 agcaaaggct gggctacagc tatgcagaca cattaaagcc gatgatcaaa acattccgtt
    37381 cttacttcag tcgtccgagc tttcaaataa aatatatgct gatgagcttt gtgtaggttt
    37441 tttacacaag tattccaaaa cttttagcct tgaccttcgc gattacattg taaacaattt
    37501 tggatttggt gattttgttt ttcgtgaccc cgataccctt gccgaaattg cccgcgcatc
    37561 ggacttgcgc cagctacaaa agctaattct caccattccc gatcgctcat tggtttacca
    37621 ttccagcagg aacgacatct ccaaatggct caatgccagg gctatatttt caattgcaca
    37681 gctctttaag cccatgcggc ttaaggattt cagaagtata gatgatgccc gaaactttat
    37741 ttactatgcc atatcggcat ataggctaag caagggtcgt agcataattg ccaagttcga
    37801 tagggaaacc tttgacgagt ataagttttt ttcacgtatt ggcgatggat caattggggg
    37861 aaaagcccga gggttagctt ttgtaaacca ggtaatccgg aactataatc ttttcaataa
    37921 gtttgaaaac actataatta ccattccaag aaccgttgtg ctcagtactg atatatttga
    37981 cgagttcatg gagtctaata acctttactc tattgctttg caagatttgg atgatgaggt
    38041 catccttgaa gccttcttga aagctaaact acccgagcgt ataaaaatag accttcggaa
    38101 agtcatttca gtatttcaaa ggcctctcgc ggttcgttcc tcaagcaaac ttgaggattc
    38161 ccattatcaa ccgtttgctg gaatctactc aacctacatg gttccccatt ccgataaccc
    38221 cgatcaaatg ctccaacact tagccaaagc agttaaagct gtgtatgcat cggtattcta
    38281 taaggccagc aaggcgtata tgaatgccac ccaaaacgtg attgacgagg aaaagatggg
    38341 ggttataatt caggagatct gtggaaacca gtatggtaac atgtatttgc caaccctttc
    38401 cggggtagct cgcagtatca acttttaccc cattggttcc gagaaaactg aggatggtat
    38461 tgttagcgtg ggttttgggc ttggcaaact tattgttgat ggtggggtag cgcttaggtt
    38521 ttctcctaaa tatcctaaaa agatactgca actttcatcg gttgaaatgg cgctccgcca
    38581 aacccagaag aacttctacg cccttaatgt tgatcccaac agctttgaac caaaggttga
    38641 cgatagctca aatttgctaa atcttgatat aagggaggcc tcttctatac ccgatttccg
    38701 tttaataaca tctacctacg atatgcactc aggagtactt cgcgatgggt attacgatga
    38761 gggtttgaag ctggtaactt ttgctccaat cctaaaacac gataccttcc cactggccaa
    38821 gattttatcg gaactcctcg agataggcga gcgtgaaatg aataatcata tcgagattga
    38881 gtttgcagca aacctgaacc ccccaagcgg tatgcctcga cttttcaact ttctgcagat
    38941 acgccctata gttgatagcg atcaaacgga gctggtagag tggaacgaga tggatataaa
    39001 taaggccata ctctactcaa agtcggcatt gggacacgga aaggttaagg atatatatga
    39061 ctttgtttac attaaaccgg aaagctttaa tccggccatg accaaggaaa ttgctcatga
    39121 gctcgatgag ctcaataaaa ggtttatcga tttgcaaagg aattacgttc tggtaggccc
    39181 aggccgctgg ggtagtagcg atccatggtt gggtgtacct atcaaatggt cacaaatttc
    39241 acaggcgcgt gttattgttg aggccggtct tgagaacttc agggttgatc ctagccaggg
    39301 tacccatttc tttcagaacc ttacctcgtt cagggttgca taccttaccg ttaaccctta
    39361 cttacacgat ggttcgtacg acattcagtt cctcaactct attcctgcat ggttcgaaac
    39421 aaaatatata cgatgcataa ggtttgaaca accgcttgta atacaggttg atgctaaaac
    39481 aaaccaaggt gttatattaa aacctgaaaa cggttgattg gttttaattt ttaagtaata
    39541 cgccaaaaaa atgtttaata tcatattttt tcatggattt tttcttttga aatgtcattt
    39601 taataaatca attttgaaaa actttttaag gggataattc ctcgcaataa aaaaactgag
    39661 tttaacaact aaaaacagtt agcgcaatga atgtaaataa attaatggct gagcttgaag
    39721 ctaaacaccc gggtgaggtg gaatacttac aagcagttcg tgaagttctt gagtcaattg
    39781 aagaggttta caaccagaat cctcaatttg aatcggcaaa gattattgag cgtatggttg
    39841 agcctgatcg tattttcacc tttaaggtgc cctggatgga cgatgagggt aatattcacg
    39901 ttaacctggg ttaccgtgtt cagttcaaca atgccattgg accttacaaa ggtggccttc
    39961 gtttccaccc aagcgtaaac ctttcaatcc ttaagttctt aggttttgag caaattttca
    40021 agaacgccct tacaaccctt cctatgggtg gtgctaaggg tggtagcgat ttcaacccta
    40081 agggccgtag cgatgctgaa attatgcgct tctgccagtc gttcatgctt gagctctgga
    40141 agcatattgg acctgaaacc gatgtacctg ccggtgacat aggtgttggt ggccgtgaaa
    40201 ttggttactt gtatggaatg tataagaaac ttgccggtga gaacaccggt gtactaaccg
    40261 gtaagggtat taactggggt ggatcactta tccgtcctca agctaccggt tttggtgcta
    40321 cttactttgc tcaggaaatg cttgctacca aaggcatgtc gtttgcaggc aaacgcgttg
    40381 caatttctgg ttttggtaac gttgcttggg gtgctgccct aaaggttacc gaacttggtg
    40441 gtaaggtggt taccatttcg ggacccgatg gttatatcct tgacgaagct ggtattagcg
    40501 gcgaaaagat tgattacatg ctagagcttc gcgcttcgaa ccaggatatt gttgagccat
    40561 tcgccaagaa attccctggt gctaagttct tcgccggcaa acgtccttgg gaagtaaagg
    40621 ttgatgttgc tatgccttgt gctacccaaa acgaacttgg caaggccgat gctgaagccc
    40681 ttgttaagaa cggtgtagtt tgcgttgccg agggtgctaa catgccttgt actcctgaag
    40741 ccattgaggt attccagcac aacaagattc tctttgcccc tggtaaagct gtaaacgctg
    40801 gtggtgttgc tacttcgggt cttgagatga cacagaatag catgaaactc aactggccaa
    40861 gcgaagaggt tgatgctcgt ttgcaccaaa ttatgaagag cattcacgag gcttgcgtta
    40921 aacatggtac tgaaaaagac ggttatgtta actacgttaa aggtgccaac attgccggtt
    40981 tcttgaaggt tgccaacgcc atgctcgatc agggtttaat ctaagcaaaa ccaaatgttt
    41041 tgttaacttt aagggctcag caatgagccc ttaattttca aacacgtatg gtatgagcac
    41101 tacatctaca attatctata agggtgattt acgaaccgag gcaactcaca cacgctccgg
    41161 ggtaaccatt acaaccgatg ctccagagga caatcagggt aaaggtgagt gtttttcacc
    41221 aaccgatctg cttgctactt cgttgggttg ctgcatggtt acaattatgg gaattgcagc
    41281 acgtacccac ggatttaata ttgatggcac aaaaatagat gtgcaaaagg ttatgggaac
    41341 aaacccacgt agggtggttg aggtaataat tgacctatac ttcccgcaca acaactatac
    41401 tgctaaagag cgaaaactgc tcgaggcttc agccaaggag tgccctgtgg ctcaaagtct
    41461 tcatcccgat ttaaagcaaa caatccggtt tcatttcccg gaataagaaa acgcccgcta
    41521 gggcgttttc ttttgttata gactttgttt gctttctgcg gaaatgtcct tgtttacttt
    41581 cttgattaaa ccctgaagta cgctgccagg acccaactca acaaattcgg tagcaccgtc
    41641 ggcaatcatg ttttgaacgg tttgtgtcca cctaacaggt gcagttagct gggctacaag
    41701 atttttcttt atctcctcag gacttgaggt gggtttggca gttgcattct ggtaaatggg
    41761 acaaacaggt tttttaaatg gagtagcatt gatggctgct tcgagttcct ggcgtgcagg
    41821 ctccatgagt ggcgagtgga atgctccgct aacggccagt ttaagtgccc tttttgcacc
    41881 ggcctctttt agtttttcgc aggccatatc gacacccttt accgaacccg atataacgag
    41941 ttgacccgga cagttatagt tggcagcaac tacaacttcg ttaatggagg cgcaaatctc
    42001 ttctactttt gcatcatcaa gcccaagcac agcggccatg gttgagggct gaagttcgca
    42061 agccttttgc atggccattg cacgtgccga cactagtttt aaaccatcct caaaactcaa
    42121 agcgcctgct gctactaacg ctgagaattc tcccaatgag tg
//
LOCUS       DAOOWJ010000007        40760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1019_length_40760_cov_7.871195, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000007 DAOOWJ010000000
VERSION     DAOOWJ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 40760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40760
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1019_length_40760_cov_7.871195"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /gene="clpX"
                     /locus_tag="PLW22_01505"
     CDS             complement(<1..918)
                     /gene="clpX"
                     /locus_tag="PLW22_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOU73408.1"
                     /translation="MKNTDKCSFCGRPRREVNLLIAGLDGFICDLCSEQAHLIVKEEL
                     KKKGEFKLQKNKLLKPLEIKKFLDQYVIGQDEAKKFLSVAVYNHYKRLLQADGGDDVE
                     IEKSNILLVGETGTGKTLLARTIARMLHVPFTIVDATVLTEAGYVGEDVESILTRLLQ
                     VADYNVEAAEKGIVFIDEIDKIARKGDNPSITRDVSGEGVQQALLKLLEGSIVNVPPQ
                     GGRKHPEQKMIAVDTKNILFICGGAFEGIQKKIAQRLNTTVVGYGASKTREVIDKDNL
                     LQYIAPQDLRAFGLIPEIIGRLPVITYLNP"
     gene            1056..1907
                     /locus_tag="PLW22_01510"
     CDS             1056..1907
                     /locus_tag="PLW22_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU73409.1"
                     /translation="MKLSLFNITNFKVDGGAMFGVVPKVLWNRVYPSDANNLIPLSLK
                     SLVVEVNGRVVLFDNGIGNKQDEKFMSHVYAFGGEGLIDGLAQRGYKPTDITDVVLTH
                     LHYDHCGGGVYRDCEGNLKLTFPNARYHVSRAQWEWAINPNPREADAYLPENLLPMRD
                     LGALNLIEVEGEIIPGIELRFFNGHTRGQIIPIISYRERKLVFVADLFPFRSHIPLPW
                     IMSYDVEPLVTLKEKETFLKESLEGEYIYIYQHDYFADCSELQLTPKGIRATDGFTFE
                     ELMEKLK"
     gene            complement(1887..2348)
                     /locus_tag="PLW22_01515"
     CDS             complement(1887..2348)
                     /locus_tag="PLW22_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73410.1"
                     /translation="MKPLKKRKSNGFNFYSIAATPYLKVNSAFALILLLVFIYSLVFS
                     QNNHPIPAALTQLTGIVPPSKGLSAAFSQIVRLNFTNAYILNPYSLRVFSFFLIQFIL
                     RVGLIVTELRFPNSVTTSTIAVDILISLTLLIYCFYPLIEFTFKILLQLLH"
     gene            complement(2320..2868)
                     /locus_tag="PLW22_01520"
     CDS             complement(2320..2868)
                     /locus_tag="PLW22_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73411.1"
                     /translation="MNVEVKTTAGQAMGIVGIVLAVISLILAFIPCIGFVALLPAALA
                     LIFSIISIIQATNGYGSKGLGIGALIVSVLSIFLAVMWLTIISGGFSVLKDLERHPEK
                     IEIFGKELEKELNDANVQIRIETDSMVKTLRQLEGESDSIKVITIKKNKVSKGDDSQG
                     ARITIDTDSVQIRIEATEKKKK"
     gene            3076..3618
                     /locus_tag="PLW22_01525"
     CDS             3076..3618
                     /locus_tag="PLW22_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73412.1"
                     /translation="MKKTILISVFVAINMMSLGQVFNTGQTLKPKTFSVGIEPAVLIN
                     GSNEFMLFLHGGYGLTKGIDVGITAGVLGPENYFGANVEFVLSKNISLAGGAHSFGSF
                     GLDGTLNLTFGLAKGVRLITGLDTDINFPDGGDAQFLLWIPAGVDIGLRKNMSFILEA
                     EIGLTDPAYNLIGGGVVFYF"
     gene            complement(3615..5204)
                     /locus_tag="PLW22_01530"
     CDS             complement(3615..5204)
                     /locus_tag="PLW22_01530"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU73413.1"
                     /translation="MEPSSLVGKNIKVKPFWHVFHWVVLFMLIIYAVKANAYEPDSLL
                     RQNISISIRTTTLFKALNQIGDKAGCFFIYDSRDVESNRRVKSIEWSDKPLNDLLNQV
                     IGDTLVKYRVIGKHILLYRPKLSDTLKAQSQEQNIKYVQLSGRILDKETAQPVAFATV
                     GIPSLAIGTISNLDGVFTLRIPKQNTDSSIAISHIGYIQRKVPAGLLTAKTFEIFLEP
                     SFIPVQEIFIRNIDARNTVREAVNKRSQNYFQQDIYITAFYREGVLRNKRILSYSEAF
                     AKIFKSSYSFDSRYDQVKLIRFRKMEQANRRDTLDVKLQAGVKATLDIDIMKNLPDFL
                     DPEFTQYYDYTRTDIVVYDNKSAYAIGFKQKPEITDPLFAGLLYIDTETLTLLAAEFE
                     INPDMVQKTASQLVVKRGRGIRVQPQKIKYLVKYQPQDGKWYVNHVRGEISLKVQTRF
                     WPFANTFDLFFEYVALQVETLEVKRFPKRETLKTTEVFSNTQAEYDPNFWNDLNYIEP
                     EESILKALGRITPKIEMVTEL"
     gene            complement(5179..6162)
                     /locus_tag="PLW22_01535"
     CDS             complement(5179..6162)
                     /locus_tag="PLW22_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOU73414.1"
                     /translation="MNSKKSYQTYVAKYLSNEMALAAKIWFYLKNQINPKTKHELNTL
                     KVFFNSMKVNEKNKDIEFDTGKAWQKLQERIKTESYTGQSRTAFIPNLNYFAVAATLL
                     VMVALSLSVWFYYNPKTITLANMTQSSTIIRTLPDGTQIFLGENTILEYPVRFAGKTR
                     TVKLSGEAYFDVAKNGSKPFIIKTSHADVKVVGTSFNLKTSNNEVHLKVTEGKVKITL
                     ATNQKSALVQAGEAAIASKNDIVTYTQPAGETIKSAMKLLMFQDETLDNIVRVINSTY
                     GSNIKVLGDDLKDMRISVTFDNDISSIVNILSVSFNLKVTRSPDGTIIISR"
     gene            complement(6190..6717)
                     /locus_tag="PLW22_01540"
     CDS             complement(6190..6717)
                     /locus_tag="PLW22_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOU73415.1"
                     /translation="MVLASTNPNEPDLKEFEKVFRKYYEPLCRYAYSIVRDADEAEEI
                     VQEFFYTFWLNRNRISIRLSLKSYLYRSIRNSALRYLQHKNIEDQYRTQDYALNHVES
                     PEQVIQGNEIAGIIDKTLKGLPERTRQIFLLNRINGLKYKEIADLLKISIKTVEADMG
                     KALLELRLQLKRAGF"
     gene            complement(6799..8094)
                     /locus_tag="PLW22_01545"
     CDS             complement(6799..8094)
                     /locus_tag="PLW22_01545"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU73416.1"
                     /translation="MLNLILSAMKTLNALIGKSLRYSATAVALIISAVLTAQEVDYNL
                     VNGVIKDAKTKERVVFATISVPGTTIGTVANADGEFTLKVPKTVKSNVFEITHLGYQN
                     TQFPIVNGTSGKDKVYLIEPSSLEIQSVVIRPMDARTILEGALKNIDKNYSDKPVSFT
                     AFYRESIKQRREYISIAEAVAEIYKAPYRYTLETDKVKIVKGRRGENVKKADTLMVKM
                     QGGPHVSLYLDVIKNPELILTREDLPYYNYEVVDVVKIDNEANYVISFTPNTKLEYPL
                     YQGKLYINTENLAITMAEFSIDLSEPEKVAQIFVRKKPAGLIFEPISTNYLVTYKKVN
                     DKYHVNYMRSEVKFRADWRKRIFKTNYTIMSEMAITERNFDNVNKITFKESFKPNLIL
                     ADRVNEYFDENYWGSYNTIEPDESIQSAISKFNKRFRKQ"
     gene            8450..10255
                     /gene="rho"
                     /locus_tag="PLW22_01550"
     CDS             8450..10255
                     /gene="rho"
                     /locus_tag="PLW22_01550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOU73417.1"
                     /translation="MYDILELNKKLLPELREIAKNLNIKKVENFKKQELIYKILDQQA
                     INASLSADDERKKDHRKPFRKPEKQVEVKREETQPVAPKKEEPQAAKPDEATSSQEAI
                     NERNKRKRIIRAKEVRKEFSATPTNSSSEEKERSNKTTIKFNEKAVILPELDTETHSE
                     TILLSNDEEDTVIPMVTEEAQAEALVSEKPEQEARASVTEVVEPVKEENNAQPIPSQE
                     RRPGSERFDFDGIVATTGVLEIMPDGYGFLRSSDYNYLSSPDDVYVSQSQIKLFGLKT
                     GDTVTGTIRPPKEGEKYFPLIKVIEINGRDPEFIRDRVPFDYLTPLFPNEKFKLIGNG
                     MNLLSARIVDMFAPIGKGQRGLIVAQPKTGKTILLKDIANSIAANHPEVYMIVLLIDE
                     RPEEVTDMARSVNAEVIASTFDEPAERHVRVANIVLEKAKRLVECGHDVVILLDSITR
                     LARAFNTVQPASGKVLSGGVDANALQKPKRFFGAARNIENGGSLTILATALTETGSKM
                     DDVIFEEFKGTGNMELQLDRKLANKRVFPAVDIMASSTRREDLLLDKEVLQKIWILRN
                     YLTDMNSVEAMEFLRDRLVKTATNEEFLWSMNSDF"
     gene            10373..10900
                     /locus_tag="PLW22_01555"
     CDS             10373..10900
                     /locus_tag="PLW22_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOU73418.1"
                     /translation="MTLPSGNYTKEELSWFRSIFDQYYESIRHFAYYKTGDVDLSDDI
                     VQETFLKLWDMRGKVNNDTVKSLLYTIAGNIIKNHYKHLKVVYSFQVAVKGDMTSNPN
                     SADERIRMDQLNKQLQEVLASLPDGQREVFLMNRIDGLTYEEIAARLGLSVKAIEKRM
                     SEALAAIRQKFGYKI"
     gene            10897..11778
                     /locus_tag="PLW22_01560"
     CDS             10897..11778
                     /locus_tag="PLW22_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOU73419.1"
                     /translation="MKRDRNINSTDSGFDSNPIEFNISVPPSNRTKEQAWDMLMQTIE
                     GKHAEPARVVTFQPHYKWLLAAASVIILMLVGSLFILNTESRFETMQAQTLEHTLPDG
                     SVVHLNASSWISYKKSYGWFGRNVRMEGEAYFKVKSGKGKFVVTDNQKQKVVVTGTEF
                     SLVTRGDSFEVRCFEGSVNVLISGLKPVGVKKGESVTKTNGMLIHSKQSDKELTKPVW
                     VTHEFEFAEEPLSQVFQQISNFYNIQISAEGFDPASRYFSGTIPAGKATDVLDVISIS
                     MGLSYTVNADSTVYTFR"
     gene            11832..14483
                     /locus_tag="PLW22_01565"
     CDS             11832..14483
                     /locus_tag="PLW22_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU73420.1"
                     /translation="MSRIKYLLISLLVGFALQIQQATYAQKVKITESFSNQSLDQILS
                     ALSGKYNIKFAYDPVALNQISFTGQFRNDSPDKVLKQLLKDSGFEFIALNNVYVIKRA
                     EKPIVEEKNKPIQGIVRDRISGEALPYASIRVVDNDLEATTNTDGFFTIPDVKSDSVS
                     IEVRYLGFEPLQMRVARVQYNSKPIVVELDPKKFILADVEVVRNITSMLATGDRPGEM
                     VFDTKKVGDIPGISGIDILAPLQLLPGVDGTTESLSGLMVRHSPADKNLISYDGFTIY
                     HINHLFGAFSSLNSKAIKDVRLYRGGFDSRWGGRASSVIEITGKTGNENSVSVDAGTD
                     QLAGDFTFEGPLGKHASFIVSARRSYTDFYRSPQFLNLFESVASDLMLSKQNFTAFGS
                     DPDAPSYLFYDANAKVSVKPSNNDILSVSGYVGRDRLNFLQMVSSPFINENALWGNSG
                     AGARWAKQWNSWLYQNFTVGISEFTSNYQHYDSTLKRRGIQPVYDTIVRNYATETGLN
                     DLSVNLNAQVTLSNQIILELGYQSNLVNSKSSENYYHAVNSLPVADTLRQYDFNSRVN
                     TGWLQFNFKTNGLKSLVIGGRVSQHNLTDQYYVEPRVQMVISPTSKLDLKFAAGLYNQ
                     FINRIYLTGNGVRYLWVASDEKTFPVVKSKHFVTGLNYTSNGLTLDIEGYAKQTEGLS
                     YVQTVIRRTSTNRFVEQSKIFYVDSRSLGVDVLVRKTWQNAEGWVSYSLSKAFNQSST
                     LNGGDEYYALDDHLHELKLVGLYKWRKWRVVGTWIFGTPKPWDELLLTPSLTLSPDYE
                     KNSSRLEPYHRLDAGLSYLAKVSVSGELEVGVKVFNLYNRDNMLARPYTLTDTPISDY
                     LQGKSIIEYHDIYGYSITPTFFFNIKF"
     gene            complement(14576..15379)
                     /locus_tag="PLW22_01570"
     CDS             complement(14576..15379)
                     /locus_tag="PLW22_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73421.1"
                     /translation="MENKVLFTDSVVANFCKATVDDNPIHDPVFMYNQNKGVVVPGML
                     LFSTIVNLLHRKTDEYFNYYKLIFGNVICTNEPIDLGFETKGDQNYLYAINGHDMFST
                     KNERSVVLKNIEPKVLDLDGIVRAMPFHKTQLETFRELTNSLNNTLSDFLFTIAYASA
                     ALFKAIREPITEVETEINRLLNKAINPDQVSPFYQTLEIFRVNQHAELNPEGTIEYNI
                     RFDREKANKTYIAQVTCMHNNQCFYQSVYRLVAIPDKLIMRMVKDRDKV"
     gene            15657..16532
                     /locus_tag="PLW22_01575"
     CDS             15657..16532
                     /locus_tag="PLW22_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOU73422.1"
                     /translation="MTRDGKIERLPENIERLLDSYNVPAGKMRSEAWEAIFEKIDHKN
                     FEEDSKGRVIQLTLTRVAVAASVLVALMLTSYIYLYRMGNVHVQLARGEHGVVYLPDS
                     SRVTLNAESKITYNKNRWFFDRSVSLTGEALFEVSKGRRFKVVTTGATTTVLGTVFNV
                     FSRNGLVKVTCFEGKVKVVATNSKSQTLLTKGMEAKTMGYALDVNKTQTTKAESKPAW
                     TNNEFFFQNAPLSAVIEELERQFNVDIFLNTDTSRFYTGYFKRTNLDEALNLVCIPLD
                     LKWDYSGEIIVITSK"
     gene            16574..17401
                     /locus_tag="PLW22_01580"
     CDS             16574..17401
                     /locus_tag="PLW22_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73423.1"
                     /translation="MKRTSLIFAAGLMLMAAFTLSSCQKDEEVDSVVTLASEDADVDT
                     YYDDVMTEVDEITLAGDSKDSQLMYATLGGQGTRTRKTSWDGNCRIDSLTYENFVNAN
                     SRYERVKNGLIVIRTCGQYNGAEFERTVTFKNFTINGNLIEGTKQIRKTAEHEYQITL
                     TNGKITFTDGTTYTRTVERTRTWVEGYDTPFYIWDDVYTLEGVATGVNRRGYTYTHQI
                     TSALMFKLSCRWLVQGVIDLTVGDKQATIDYGNGDCDNIAYVTVNGKTYEVKLRGGK"
     gene            17613..17813
                     /locus_tag="PLW22_01585"
     CDS             17613..17813
                     /locus_tag="PLW22_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU73424.1"
                     /translation="MSTIVVDEARCPQNHPCPVVRYCPTGAIKQRSWNSAPEIDKENC
                     KNCGLCVRICGYGAFTFADNGR"
     gene            17872..18378
                     /locus_tag="PLW22_01590"
     CDS             17872..18378
                     /locus_tag="PLW22_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOU73425.1"
                     /translation="MRKITAFFPVLLVAAFALGACNGTAAKQNEGEPVTALKPAKEKY
                     AIDITRDFFLTKILDYTKGQENMKYLGDKPAIVDFWASWCGPCRIAGPILEELAKEYD
                     GKIYVYKVNTQNEQQISAELGIQSIPTFIFFPMDGKPFATSGIAQTPEETKKMFKEII
                     EKELLKKK"
     gene            complement(18441..19595)
                     /locus_tag="PLW22_01595"
     CDS             complement(18441..19595)
                     /locus_tag="PLW22_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOU73426.1"
                     /translation="MQVKDILKKAINLESLTLEEGLYIYTQASTPDLMYVANELRKIH
                     VPGHKVTWQIDRNVNITNVCISGCKFCNFHCGLQSDSAFITSIAEYKVKIEELERYGG
                     DQLLLQGGLHPRLGVEFYCSLFKELKSMFPNIKLHALGPPEIAFIAKKEGMTYRQVLE
                     ELVEAGLDSLPGAGAEILVDRVRKQISPGKPDSQAWLDVMAQAHQMGLVTSATMMIGH
                     VETLEERVEHLIKLRNLQAQKPADAPGFLNFIVWTFQDEGTKLQREGVLNTVTTEEYV
                     RTIALCRIMLNNIPNIQASWLTVGIDTAQLCLHAGANDFGSIMIEENVVSSAGAKYKM
                     DAYGIQKAIVDAGFEPQLRNQRYEYIVLSDCEFSRIYSIEELKQNTSPLL"
     gene            19728..20780
                     /gene="pdxA"
                     /locus_tag="PLW22_01600"
     CDS             19728..20780
                     /gene="pdxA"
                     /locus_tag="PLW22_01600"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOU73427.1"
                     /translation="MSNEKIRVGITQGDINGIGYEVIIKTLMDPRMDEMCTSIVYGSP
                     KVAAYHRKALNIENFSFNQIKDASEANPKRPNIVNCMDENVRVELGKSTEYGGQGALL
                     ALQAAIADLKAGKIDVLVTAPINKANIQSQSFSFPGHTEFLANEFGVKSALMLMVSDT
                     MKVGVVTGHIPIADVPKNITVDAILNKLRLLNETLIKDFAIRRPRIAVLGLNPHAGDS
                     GVIGNEEETIILPAIKQANEEGIVAMGPYPADGFFGSDRFTRFDAILAMYHDQGLSPF
                     KVINFDTGVNYTAGLPIVRTSPDHGTAYDLAGHNLANPNSFRQAVYLAIDIFRNRQLH
                     KELTSNPLQSVDPETA"
     gene            20793..21377
                     /gene="ruvA"
                     /locus_tag="PLW22_01605"
     CDS             20793..21377
                     /gene="ruvA"
                     /locus_tag="PLW22_01605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOU73428.1"
                     /translation="MYEYIQGSLAELTPTHAIVDAGGVGYFVNISLQTYSQLEGKTQV
                     KLWLHQIIREDAHLLYGFTDVREREIFRLLISVSGVGASTARLILSSLTTDEIQAAIT
                     TENVNLIKQVKGIGLKTAQRIVVDLKDKVSIGEATVSQILSTSNNTLKSEALSALVML
                     GFARSAADKVIDQVLKEMGQMPVEALIKHALKRL"
     gene            21400..28752
                     /gene="sprA"
                     /locus_tag="PLW22_01610"
     CDS             21400..28752
                     /gene="sprA"
                     /locus_tag="PLW22_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOU73429.1"
                     /translation="MRVKHLSHTHTIRIIGILTLVTITITRAFSVPIPQQDTLNLPFP
                     IKQPGIWSIDTQYSPLYLSSPSNISEEVVFDPINNQYIIYRKVGNTTIETPRVLSADE
                     YRSYRVEKAMREYWRQKQTGEFVGKGDGILPRIQVGGETFDRIFGSNTIEIIPQGNAE
                     LVFGVSSAKTDNPALPIDQRRNTTFDFQSKIQMNVSGKIGEKLKMEVNYNTEATFDFE
                     NNVKIEYNGFEDEIIQRIEAGNVSLPLPGTLITGSQSLFGIKTQLKFGKLNVTSVVSK
                     QNGQTQVVEIKGGAQTRDFQLKADEYDANRHFFLSHYFRENYNRALMNLPIINSGVQI
                     TKIEVWVTNKQANFENSRNIIAFADLGEAQNNIFASNIFAQTGSGPASNGLNNLYELM
                     TTTYAGIRDVAEVSNVLLPLESQGFTGGRDYEKIEYARKLSPNEFTINQALGYISLSS
                     ALNSDEVLAVAYEYTYNGQTYKVGEFSTDGVEAPKALILKLLKGTNLSPKMPTWRLMM
                     KNIYSMNAYQISKDDFRLDIFYQDDATGTPINYIKEGNIAEVPLIRVLNLDNLNNQLD
                     AFPDGVFDFIEGVTINPANGKIIFPVLEPFGRDLASRIGDPTIAQKYIFQELYDSSLT
                     KARQVADKNKFLIGGSYKSAGGSEIFLNAPNIPKGAVVVTAGGVKLQEDVDFIVDYNL
                     GTVRIINQGLLESGTPIKVAVESNALFSLQTKTLVGTHFDYRFSDNFNVGATIMNLTE
                     RPLTQKVSFGNEAVSNTIWGFNTSYQTESKFLTKMVDFLPFIQTKEKSTITFDAEFAQ
                     LIPGHSRAIGKAGTVYIDDFEGSSTSLDIRSWVEWKLASTPQGQPDMFPEASFSNDLR
                     YGYNRAHLAWYYIDPLFLRNTSLTPSYLRNNKELQGNHFVREIFERELFPNKNTPQGQ
                     PTNISVLNLAFYPTERGQYNFNTTELDANGRLIDPQRRWGGIMRNLSTTDFEASNIEY
                     IEFWLMDPFVYDTLSSGGDFYINLGNISEDILRDGRKSFENGLPTSSEELSKTDQTAW
                     GRVPTKQYLPVGFDNDQAKRPYQDIGLDGLSSSVDLDGDGILDEQFFFSEYINQLRNK
                     ITNSDVLAGFESDPAGDNFRHYLSGVYDQQRATILDRYKYFNGTENNSPVATGTTSQQ
                     SYSTPDVEELGTDNTMNENEAYYQYRISLRPQDMEVGKNFITNVVTGDKDKILNKAVK
                     WYQFKVPIAEYEKVVGPIEDFKSIRFMRMFLRGFQDTVILRFATLHLVRSEWRKYNYS
                     LIQGQEGMPVGDLPSGSLSIATVNIEENDKRTPVNYVLPPGVDRVIDPSQPQLIQLNE
                     QAMEYRVTDLADGDARAAFKNVNIDIRQYKRLKMDVHAEAIEGYPLNDYEVNAFIRIG
                     TDYNTNYYEYEIPLKLTPYGFYYNSRERDREAVWPRENVIDIDLDKLVDVKVARNNAM
                     QKPGSLITLNTVYVVQSGNSKLKVTGNPNLSNVKVIMIGIRNPADDRMPKSVIAWFNE
                     LRLTNFNDQSGWAANARLSAKLADLGMVTVAGTTITPGFGSIEKKVNERSKETVYQYD
                     INSTLELGKFFPASFNIKIPMFIGFGESMANPQYNPLDPDILLDDALRNASERERDSI
                     KRIAQDYTRRRSINFTNVKIDKMGSKPRLWDPANISVSYSYSEIFSRNVRIDHRIQRN
                     IRGAFTYNYNRQPKYISPFSKIKWLNSSYLALIRDFNFNLFPTQLSFRTDLNRNYFEQ
                     QFRNIQNPNQVFLPTFSKDFMWNRNYSLNWDITQSVKFDFVATATARIDEPEGMVDRY
                     RDRDRYEQWKDSVWQNIRRGGRNTMYNHQFNITYSLPLSKIPLLNWISITSRYTGIYR
                     WQAGPILADTSRFDPGNTIQNSNTIQLNGQVNINTLFNKVGYLKRVNQQFDQRARGAA
                     PQTRTVKYEEEKVNLRANATKVITHKLKTEKVTVKVFDPNGKEIKVSSQVRGDTKVAI
                     KADADYKNCKVVVEGKVPEKINPARFALETTLRLLMSVKSLGASYTETNGTLLPGYKP
                     GVNYLGTESFNGMLAPGWEFVAGWQDPDFAWKAATNGWLTRDTSFNTPVVFTRNENLN
                     FRATLEPLPGFRFDVSATRTLGKSNSKIFKSDGLGSYRVLSPLTTGNFSISVLTIGTA
                     FEGGKRTGSSKAFERMKQNRLEIAYRLRDRRVANPSQGYDPNVIDPVTGFPDGYGPLS
                     QDVLRASFLAAYTNTSPSKVSLSDFPLIPMPNWQLSYDGLAKMKPFKRWFKSITINHG
                     YRSLYTIGAFTSNLTYLPEDDGFSYVRNINGDFVPQNEITNVAINEQFTPLVGLDLGW
                     NNNLTNRIEIKNSRSLAMSFANNQLTEIYGNEFIVGAGYRFEDLPLIFQAEDGGKKAI
                     KSDLRLTVDFSLRDNTTILRKLVEDSNDIIAGQLISTIKTAADYRINEKVTIRLFFDR
                     TLNNPKVSRTFRTTNTSFGFSVRFELVN"
     gene            28871..29254
                     /gene="gcvH"
                     /locus_tag="PLW22_01615"
     CDS             28871..29254
                     /gene="gcvH"
                     /locus_tag="PLW22_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOU73430.1"
                     /translation="MNIPSNLKYTKDHEWIKDEGNGIATVGVTDFAQTQLGDIVFIEI
                     ETQGETLAREEVFGTIEAVKTVSDMFMPVSGEVVEVNPKVTEQPDIVNKDPYGEGWMI
                     KIKMSNPAEMNDLLNAEQYKGIINE"
     gene            complement(29292..30338)
                     /gene="thiL"
                     /locus_tag="PLW22_01620"
     CDS             complement(29292..30338)
                     /gene="thiL"
                     /locus_tag="PLW22_01620"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOU73431.1"
                     /translation="MSETQKRTELYELGEFKLIDRLTEPFAPKHQETLKGVGDDAAVI
                     SCGKGECLVVTTDLLLEGIHFDLTYTPLKHLGYKAIVVNLSDVYAMNAIPKQVTVSIG
                     VSKRFCVEDLDELYSGIKLACDSYNVDLVGGDTSSSMTGMTISVTAIGSAKEDEIVYR
                     SGAKVNDLICVTGNLGGAYMGLQLLEREKRIFLSNKAAKPKLEGYDYILERFLKPEAR
                     RNIIENLRKAGVKPTSMIDVSDGLSSELLHICKSSNVGCRIYMDKIPIDEQTARMAEE
                     FNIDMFTAALNGGEDYELLFTVPLSEYQVVDSMENVRVIGHIVEPHLGAYMIPPSGED
                     IRITAQGWNALQKE"
     gene            30434..31711
                     /locus_tag="PLW22_01625"
     CDS             30434..31711
                     /locus_tag="PLW22_01625"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU73432.1"
                     /translation="MLKKILPLLVISLLTVFIIDYFIGTAYIKLYSPEKDQKPLVEDV
                     QPILEYGIPIDSFYVTEYTVRSNQTLGKILFSLNLTGNVNEFLLSLTPDIFDVKKVRA
                     GARYKVFARKDSLQTPAYLVYEVSPVEYFVFNLTDSLYVYPFKKEIVPVRKISEAEIK
                     SSLWVDMKANGLNPMLALELSDIFAWTVDFFGLFKGDKFRVMYDELYVDSTSIGIGTV
                     YAAWFEHRGEKFYAFRFEQDSIASYWDEKGNSLRKSFLKAPLRFSRISSRFSGNRFHP
                     VLKIYRPHSGVDYAAPEGTPVMALGDGVVIDMGYDKAAGNYIKIRHNSVYTTGYNHFS
                     RFAKGIARGARVKQGDIIGYVGRTGYATGPHLDMRVWMNGSPIDPLKMKSPPVEPVKQ
                     ENLETFRNVVAKLSAQLDSITPGSRIYYAVQKE"
     gene            31715..32500
                     /locus_tag="PLW22_01630"
     CDS             31715..32500
                     /locus_tag="PLW22_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU73433.1"
                     /translation="MLLIDTHSHIFSDEFDTDREAVLMRAAEIGVHYHILPNIDSSST
                     NRLLDTCNRFPQCFPLIGLHPTSVRNGFEREIYHVEEQLAKTRFWGIGEIGIDLYWDK
                     TYFIQQQEAFRHQLRLAKKLNLPVVIHSRNSFREIFDIVDQEIDNTLKGVFHSFTGTI
                     DDYKHIDEYKTFLVGIGGIVTYKNGGVNGLVNQLDPNKILLETDSPYLSPVPVRGKRN
                     ESYNLIYIADKVAELLGVTVEQVAKQTTQNAVKLFKIKIDEIG"
     gene            32487..33524
                     /locus_tag="PLW22_01635"
     CDS             32487..33524
                     /locus_tag="PLW22_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOU73434.1"
                     /translation="MKSGNTSILIIYTGGTIGMKQNPETGALAPFNFEQIEQEVPELK
                     KFGFNLSTYSFEPPIDSSDINPDFWRKLAAIIGDNYQSYDGFVILHGTDTMSFTASAL
                     SFMLEGLSKPVVLTGSQLPIGMLRTDGKENLISAIEIAASLQNGQAMVPEVTIFFENR
                     LYRGNRTTKHNSEHFNAFRSDNYPPLAEAGISIKYNYPFIHYPTQEQHLIVHTQLDSS
                     IAVLKLFPGISENVVESILTTKGVKAVILETYGSGNAPMQKWFLKKLEYAINSNIVVL
                     NVTQCKAGGVDMDKYENGLMLKRLGVVSGHDITYEAAVAKLMFLLGKGFYGKALAHEL
                     EKSIAGEITLK"
     gene            33601..33691
                     /locus_tag="PLW22_01640"
     tRNA            33601..33691
                     /locus_tag="PLW22_01640"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33635..33637,aa:Ser,seq:gga)
     gene            33765..34562
                     /locus_tag="PLW22_01645"
     CDS             33765..34562
                     /locus_tag="PLW22_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU73435.1"
                     /translation="MKKLFTLVAVLGVLSFGASTFVNAQDEPAAKPDSAMVQEQAAQA
                     ATPAAPAVEEQSLHKAIKTKFIEGGPGFMASILLTLIFGLAIVIERIIYLNLSTTNTK
                     KLLNAIEDALKNGGVEAAKEVCRNTRGPVASIFYQGLDRVHEGIEVVEKSIVTYGSVM
                     MGQLEKGLTWISLFIAVAPMLGFMGTVIGMIGAFDAIQAAGDISPSLVAGGIKVALIT
                     TVFGLIVAIILQVFYNYLLNKIDSITNEMEDASVSLLDILVKFNLQK"
     gene            34569..35000
                     /locus_tag="PLW22_01650"
     CDS             34569..35000
                     /locus_tag="PLW22_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73436.1"
                     /translation="MNSSLSTYTKVLSWLLMGVSIVITILFFLSNQDIESVEPFIDWA
                     YILFAIAAVSALVFPVVNLVRNPKNVTKSLVGLLAVAVIFVIGYVLADATPIPSPTQN
                     ADLSNPTVLIISDTGLIATYLLFGISMLLLLYTGVRSAFHK"
     gene            35059..35634
                     /locus_tag="PLW22_01655"
     CDS             35059..35634
                     /locus_tag="PLW22_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU73437.1"
                     /translation="MARKVPEVNASSTADIAFLLLIFFLMTTTMNVDTGLTRMLPPMP
                     DPNQKQDDVEVKRRNIFSVLINKSDKLLVRGEPMEVGMLKEKTKEFIMNPNSDPNLSE
                     FEEKDIPGIGKFKVSKGVVSLQNDRGTSYEMYMKVQNELVAAYNELRDELSTSVYGKP
                     FSKLAKEEQETISKAIPLKISEAEPKNVGGK"
     gene            35637..36110
                     /locus_tag="PLW22_01660"
     CDS             35637..36110
                     /locus_tag="PLW22_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU73438.1"
                     /translation="MAKFERKGKNELQAISTASLPDIVFMLLFFFMVSTTMRETTLMV
                     RSQLPEATEVNKLEKKSLVSYIYIGPPMRQFQSLFGTEPRIQLNDSFKSLDDIRDFIA
                     SEREAMKEDEKPLLTTSLKIDKETRMGIVTDVKQELRKASALKIVYAARKADKKK"
     gene            36269..37669
                     /locus_tag="PLW22_01665"
     CDS             36269..37669
                     /locus_tag="PLW22_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOU73439.1"
                     /translation="MRARTLITILLAIAMASCGKHEPKAKYVFYFIGDGMGVSHVTLA
                     KLYADSVLLDSTAMSFTRFPVTSLSTTYAHNRYVTCSAAAGTALSTGTKTSINTIGLT
                     YDRTDTLYSIAKKFKEAGKKVAILTSVSIDHATPAAFYAHVGSRGSYYDIAKQLLKSN
                     YDFFASGGFLEPYGTKTDSNAVSVYELGKVRGCQFTSSLTEVDSLVAAGAKSIVYSAP
                     NPAPSSTLQYEIDRSESDISLADITRKAIEVVDNPNGFFIMVEGGKIDWAAHDNDAAT
                     VIHEVLAFARAIDVALEFYRKHPHETLIVVTADHETGGLSIGNRENKYDFHVKLLHYQ
                     TASEERLHKLIAEFKEQNPKAKFDDLLKFLADKTGLSKDIELNENDIKMLKDAYNVAF
                     KGLKSNEKTTYSETDPISKVSIQILNSKAAVGWTSGSHTGQPVPVFAIGSGQQLFVPL
                     LDNTDIPKNIVRAAGI"
     gene            37876..39510
                     /locus_tag="PLW22_01670"
     CDS             37876..39510
                     /locus_tag="PLW22_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73440.1"
                     /translation="MKKGYLLLALLLSANIYAQDIEKGNRIPSTYDRSSITVFYMKFP
                     GENHASEVEAKFPEVKFTDKYYNNNLDALVFEAPFTRGDASVVPTKAIESFLMQKGVP
                     KSIISKWYNRKDDGSMDLDLIFDRGMFNATDAQYIKAQATKRGDNILKDYGNRLIEKS
                     YILVLDYQSILTMKEAKVEKMKGWKATVNGYLYKVKFNEETQNALYDCWIYPEDSPAV
                     RQEKVKKFQELNIPIEFVTQTVVYATASQPTEDTQLGKLIKQKTEDQLLTELVQKGYD
                     ESLYYLEMKHEDFMVKTTIHQVRPIRAKIGLKEGLKCDHRYFAYEYVYNEKTNSVEPR
                     FRGVIRATSKIVDNRQVAKGDMPTSQFYQTAGRRLREGYLIRQQNDLGLELLVGYEAG
                     EVGGIYGRIDARMGRYVGIKALFVYVEGGVQAKDYEIGFVNYENIAFLHYGAGLAKGF
                     MLTRNLELRPYIGLGQEQANQSDLSSSTLKALYLKAGANLALNLKHNIQLMGGIGSYS
                     FISNAEEGGTDTGLTWDEKFTDRKGAAVMFGIKFMF"
     gene            39531..40052
                     /locus_tag="PLW22_01675"
     CDS             39531..40052
                     /locus_tag="PLW22_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73441.1"
                     /translation="MRKVLLGFTVAVLAMVMVLPTNAQTRKERKILWNSQYNYEIEPV
                     GVGQDGTKVFKVWGYGKDVGTAVMNAKEAAVAACIFKGLPGGGGSAPTPPICSQPNAE
                     QIHADYFEKFFEVGGKYLQYIAVTNDADPSGEDRIKMKKGYKVAIYVQVMYDALRKQL
                     EADGIARRLDSGF"
     gene            40067..>40760
                     /locus_tag="PLW22_01680"
     CDS             40067..>40760
                     /locus_tag="PLW22_01680"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOU73442.1"
                     /translation="MKRILLSISLLALAVIAFGQAKKPIIMVVPSDQYCISRGYKMEF
                     NNQGLKQVLPDYKTALQTDPDLRLVITKMGQIMADRGFPLKDLEQELKNLEQERAESS
                     MLTSSSSGSEMAESPIDALKRVAKADIIMDLDFEVKRQGPQKYIVFNLKGLDAYTAKQ
                     VSGVAGAGSPSTSASPELLLEEAVLSHMDNFNAGLMRHFEDMFANGREVKVQIKVWAN
                     WGENLESEFGSDG"
BASE COUNT        11785 a         8359 c         8966 g        11650 t
ORIGIN      
        1 cgggtttagg tatgtgatta cgggtaaacg tcctattatt tcgggtatta ggccaaaggc
       61 tcttaggtct tgcggggcaa tgtattgaag gaggttatcc ttatcaataa cctcgcgggt
      121 tttgcttgca ccatatccaa caaccgtggt gttcagcctt tgggctatct ttttctgaat
      181 gccctcaaat gcaccaccgc agatgaatag gatattctta gtatcaacgg caatcatctt
      241 ctgctcagga tgctttcgtc cgccttgggg ggggacattt acaattgagc cttcaagcag
      301 tttaagtaga gcttgctgaa caccctcgcc tgaaacatcg cgggttattg agggattatc
      361 gcccttacgg gcaatcttat caatctcatc aataaataca attccctttt cggctgcctc
      421 aacgttataa tcagctacct gtaaaagcct tgtaaggatg ctttccacat cctcgcctac
      481 atagccggct tcggtaagca cggttgcatc aacaatggta aaggggacat ggagcattcg
      541 ggcaatggtt cgggctaaaa gggttttccc tgttccggtt tcgccaacca gaagaatgtt
      601 tgatttctca atctctacat catcgccccc gtcggcctgg agaagcctct tgtaatgatt
      661 gtaaacggct accgatagaa atttttttgc ctcatcctgt ccaattacat actggtcaag
      721 aaatttcttt atttcaagag gtttgagtaa cttatttttt tgaagcttaa actcgccttt
      781 ctttttaagc tcttccttaa ctataaggtg tgcttgttca gagcataggt cgcaaataaa
      841 cccatcgagc cctgcaatta gcaggttaac ctccctacgc ggacgaccac aaaacgaaca
      901 cttgtcggta ttcttcattt taccattaaa tttgctgcaa agttaatcaa taataaccga
      961 attgggttat ctatttttaa aagaacaaag ttgtaaccct aaatgttatg ataactgggc
     1021 ataaatattt tgtatcatac ataaaaaagt atatcatgaa gctgtcgctt tttaatatta
     1081 caaacttcaa ggttgatggt ggcgccatgt ttggtgtagt tccaaaggtt ttgtggaaca
     1141 gggtttaccc atcagatgct aacaatctaa tcccgctttc cctgaaatcg cttgttgttg
     1201 aggttaatgg cagagtggtg ctttttgata atggcattgg caataagcag gatgaaaagt
     1261 ttatgagtca tgtttacgca tttggcggcg agggtttaat tgatgggctt gcccaaaggg
     1321 gctataagcc gaccgatatc accgatgtgg ttttaaccca cctgcattat gaccattgcg
     1381 gaggaggagt ttatcgtgat tgtgaaggca accttaagtt gactttccca aatgctcgct
     1441 accatgtgtc acgcgctcag tgggaatggg caataaatcc aaatccgcgt gaggcagatg
     1501 catacctacc cgaaaacctt ttacctatgc gcgatttggg tgcattaaat ttaattgaag
     1561 ttgaagggga aattataccc ggaatagaac ttcggttttt taacggtcat acccgtgggc
     1621 aaatcatccc gattattagt tacagggaac ggaaactggt ttttgtggcc gatcttttcc
     1681 ctttccgttc acatattcct ctaccctgga ttatgagtta cgacgtggaa ccccttgtga
     1741 cattgaagga aaaggaaaca tttttgaagg agtcgcttga aggtgagtac atctatatct
     1801 accagcacga ctactttgcc gattgcagcg agttacaact tacccccaaa ggaattaggg
     1861 caaccgatgg ctttacgttt gaagaactaa tggagaagtt gaagtaatat tttaaaggtg
     1921 aattcaatta gcgggtaaaa gcagtagata agtagagtaa gggaaattag tatatcaaca
     1981 gctatagtag atgttgtgac agagttagga aaccttaact ctgtaactat taagcccacg
     2041 cgcagtataa attgaattag gaaaaatgaa aatactctta aactgtaagg gttgagaatg
     2101 tatgcatttg taaagttgag ccttacaatc tgcgagaacg cagccgacaa acctttactg
     2161 ggaggaacta tccccgttag ctgcgtaagt gctgcaggta tgggatggtt gttctggctg
     2221 aaaaccagag agtaaataaa aaccaaaagt aaaatcagcg caaatgctga gtttacttta
     2281 aggtagggag tagcagctat gctgtaaaag ttaaaaccgt tactttttct ttttttcagt
     2341 ggcttcaatt ctaatttgaa cagaatcggt atcaatcgta attcgggcac cctgtgaatc
     2401 atcccccttg ctaactttat tctttttaat ggttatcacc ttaatgctat cggactcacc
     2461 ctcaagttgc ctaagtgttt ttaccatgct atcagtttca attcttatct gaacattggc
     2521 atcgttaagt tccttttcaa gttccttccc aaatatttca attttttctg gatgcctttc
     2581 taaatcttta agaacagaaa agccaccact gattattgtt agccacatta cagctaggaa
     2641 aattgataga acagaaacaa tgagtgcacc aatgcctagc ccttttgaac cataaccatt
     2701 tgtggcttga ataattgaga taatactgaa aattaaggca agggcagctg gtaacaaggc
     2761 aacgaatccg atacatggta taaaggctag gatgagtgag attactgcaa gcactatacc
     2821 tacaatcccc atggcctggc ctgctgtggt tttaacttct acgttcatgg cttaatgttt
     2881 tctataacga cgaaagatag taatattttg ctgcattgtt tttttaaatc ttattttatt
     2941 taactttgtt acaaatatgc atttcaagag aagttgttga agggatcttt aacaggttac
     3001 ttattctgaa tttgtaaaat accaaaatcg ttttttctct tgctttcttt aagttaactt
     3061 ctaaatcaca taaccatgaa aaagaccatt ttaatatctg tatttgttgc catcaacatg
     3121 atgtcgttag ggcaagtgtt taataccgga caaactctaa aacccaaaac tttttcggta
     3181 ggcattgaac ctgcagtgct aattaatggg agtaatgagt tcatgctttt tctgcatggt
     3241 ggttacgggc ttacaaaggg aatagatgtt ggtatcactg ccggtgtact tggacccgaa
     3301 aattactttg gagctaatgt tgagtttgtt ctgagtaaaa atatttcgtt ggctggggga
     3361 gcgcatagct ttgggagttt tggactcgat ggtacattga accttacttt tggtttggca
     3421 aagggggtta ggctaataac tggcctggac accgatatta acttccccga tgggggcgat
     3481 gcacaatttc tgctctggat tcctgctggt gttgatatag gtttacgtaa aaacatgagt
     3541 tttattcttg aggccgaaat tggactgacc gatccggcat acaatctaat tggcggggga
     3601 gtggtttttt acttttagag ttcagtaacc atttcaattt ttggggtaat tctacctaat
     3661 gcctttagaa tgctttcttc aggttcaatg tagttgagat cattccaaaa gtttgggtcg
     3721 tattcggctt gtgtgttaga gaatacttcg gttgttttga gcgtttcgcg ctttgggaag
     3781 cgcttaacct ctagggtctc cacctgtagg gcaacatact caaaaaacag gtcgaaagta
     3841 ttggcaaagg gccaaaaacg ggtttgcacc tttagggaaa tttcacctct tacgtggttt
     3901 acataccatt tgccatcttg aggctggtat ttaaccaggt acttaatctt ttggggttga
     3961 acgcgtattc ctctccctct ctttaccacg agttggcttg cggttttctg aaccatgtcc
     4021 gggttaatct caaactcagc ggcaagtaga gttaaagttt cagtatcaat atatagtaag
     4081 ccggcaaaaa gggggtccgt aatttcgggt ttctgcttaa acccgatagc gtaagccgat
     4141 ttgttgtcgt acacaacaat atcggttcgg gtgtagtcat agtactgcgt aaattccgga
     4201 tctaaaaagt cggggaggtt tttcatgata tcgatatcaa gggttgcttt caccccagcc
     4261 tggagcttta catcgagggt gtcgcgcctg ttggcctgtt ccatcttcct gaaacgtata
     4321 agcttaacct gatcgtatcg ggagtcaaag ctgtacgatg atttaaagat tttggcaaat
     4381 gcctcagagt agctcaatat ccttttattg cgcaataccc cttcgcggta gaatgcagta
     4441 atgtagatat cctgttggaa gtagttttgt gatcgtttat taaccgcctc acgaacagta
     4501 tttcgtgcgt caatgtttcg aataaaaatt tcctgtacag gaataaaact gggttcaaga
     4561 aaaatttcaa aggttttggc tgtaagaagc ccggctggca ctttgcgttg aatgtaacca
     4621 atgtgcgata tagctatact tgagtcggtg ttctgctttg gaattctaag ggtaaatacc
     4681 ccatcgaggt tacttattgt cccaatagct aaactgggta tcccaacagt agcaaatgct
     4741 acaggttgtg ccgtttcctt atccaaaata cgaccgctaa gctgaacata cttgatgttt
     4801 tgttcttgtg attgtgcttt tagggtgtcg gatagttttg gtctataaag taaaatatgt
     4861 ttgccaataa ccctgtactt tactaaggta tcaccaataa cctggttgag taggtcattg
     4921 agcggtttgt cactccattc tattgacttt acacgccggt tgctttctac atcacgacta
     4981 tcataaatga aaaaacagcc agctttgtcg cctatctggt ttagtgcttt aaagagggtt
     5041 gtagtcctta tgctaattga aatattttgc cgtagtaagc tgtcaggttc gtaagcatta
     5101 gctttaacag cgtaaattat gagcataaat agtactaccc aatggaatac atgccaaaat
     5161 ggttttactt ttatgttttt acctactaat gatgatggtt ccatcggggc tacgtgttac
     5221 tttgaggtta aatgacaccg acaggatatt cacaatgctg ctgatgtcgt tatcaaaagt
     5281 aacagaaatt ctcatatctt ttaagtcatc acctaaaacc tttatgtttg aaccataggt
     5341 gctgtttatt acccttacaa tattgtctaa tgtctcgtcc tgaaacatca aaagcttcat
     5401 ggccgatttt atggtctcgc ctgctggttg ggtgtaggta acaatatcgt tcttgctggc
     5461 aatggcagcc tcgcctgcct gcaccaatgc gcttttttgg tttgtggcaa gggtaatctt
     5521 aaccttccct tcggttactt tcaggtgaac ttcattgttg gatgttttaa ggttaaacga
     5581 ggttcccact acttttacat cggcatgcga ggtttttatt atgaatggtt ttgagccatt
     5641 tttagccaca tcgaagtatg cttcgccact gagtttcacg gtgcgagttt ttcctgcaaa
     5701 ccttacagga tattccagaa tggtgttttc ccctaaaaat atctgagtac catcgggcag
     5761 agttcgaata atggtactgc tttgtgtcat gttggctagg gtgatagttt tgggattgta
     5821 gtaaaaccat accgatagcg ataatgctac cataaccagt agtgttgctg caacagcaaa
     5881 atagttgagg tttggaataa aggcagtgcg gctttgtccg gtataggact cggtttttat
     5941 cctttcctga agtttttgcc aggctttacc ggtatcaaat tcaatatctt tattcttttc
     6001 gttcactttc atggaattaa aaaaaacttt aagtgtatta agttcatgtt ttgttttagg
     6061 attaatttgg tttttgaggt aaaaccaaat tttagcagcc aaagccattt cgttgctaag
     6121 gtattttgct acataggttt ggtatgattt tttactattc atggttcttg gcattaggca
     6181 ttacttactt taaaaacctg cacgtttaag ttgtaacctt agttcaagca aggcctttcc
     6241 catatcggcc tcaactgtct taattgaaat cttaagcaaa tcggcaattt ccttatactt
     6301 taaaccgtta atcctgttaa gcaagaatat ttgcctagtc cgttcaggta accctttcag
     6361 ggttttatca attataccgg caatctcatt cccttgaata acctgctcag gcgattcaac
     6421 atgattgagg gcgtaatcct gggtacggta ttggtcttca atgtttttgt gctgaaggta
     6481 acgtaaggca ctgtttcgaa tggaacggta taggtaggat tttagcgata gcctgataga
     6541 tatccggtta cggtttaacc aaaaggtgta aaagaactcc tgaacaattt cctcggcctc
     6601 atcggcatcg cgtacaatgg aataggcata gcggcaaagt ggttcgtagt atttccggaa
     6661 tactttctcg aactctttaa ggtcaggctc gttggggtta gtggaggcca ataccataat
     6721 caaaacagtt gaacaaatac aaaattaata aaacaaagca aagaaagggc agggcttacc
     6781 cctgcccagt tccaatcctt actgcttcct gaaccgcttg ttaaacttac tgattgccga
     6841 ttgtatcgac tcgtcgggtt caatggtgtt gtatgaaccc cagtaattct catcaaagta
     6901 ctcgtttacc ctgtcggcca gtatcaagtt gggcttaaac gactccttga atgtgatttt
     6961 attaacattg tcaaagttcc gttccgttat tgccatttcc gacattatgg tgtaattggt
     7021 tttgaatatc cgtttacgcc agtcggcacg gaatttaacc tcgctacgca tatagttaac
     7081 gtgatactta tcgtttacct ttttatatgt aaccaggtaa tttgtgctga tgggttcaaa
     7141 aattaaaccg gcaggttttt tccgaacaaa aatctgggct accttttctg gttcacttaa
     7201 atcgatgctg aattcagcca ttgtgatagc aagattctca gtatttatat acagtttacc
     7261 ttggtaaagt ggatattcaa gtttggtgtt aggtgtaaag ctgataacgt agttggcctc
     7321 attgtcaatc ttaacaacat ccaccacctc gtagttgtag tagggtaagt cttcacgggt
     7381 gagaattaat tcggggttct ttataacatc aaggtaaagc gatacatgtg gccctccttg
     7441 catttttacc atcagggtgt cggctttctt aacattttcg cccctacgtc cctttacaat
     7501 ttttacttta tcggtttcaa gggtataccg gtaaggagcc ttgtatattt cagcaacggc
     7561 ttcggcaatg gagatatatt cacgcctctg cttaatggat tcccggtaaa aagcagtaaa
     7621 gctaacaggt ttatcgctgt aattcttgtc aatgttctta agggcacctt ccagtatagt
     7681 tctggcatcc attgggcgaa ttactaccga ttgaatttcg agcgatgaag gttcaatcag
     7741 gtatacctta tccttgccac tagttccgtt aactattggg aattgagtgt tctggtaacc
     7801 caggtgagtt atttcaaaaa cattcgattt aacagttttg ggaaccttaa gggtgaattc
     7861 gccatcggca ttggcaactg ttccaatagt tgtgccgggc acgctaatgg tagcaaatac
     7921 cacccgttcc tttgttttgg catccttaat tactccgttt acgaggttgt agtccacctc
     7981 ttgtgctgtg agcactgctg agattatcag cgcaactgct gtagcgctat acctcaggct
     8041 tttcccaata agtgcattca gggttttcat ggctgaaagt attaagttta acataaattg
     8101 cttacctgtt tctgtaccta tgatacacga gttagctttt accctataac ttttttgatt
     8161 tttcctttaa cttacctaaa aattcaatga aattcaatga gttgtcaaaa attgcatcat
     8221 ctgttttttt gtttaagcaa aacattttat ctttaagtgt tgtttgaagc aagtaattag
     8281 ttcttccaat ttttattcaa atctcaattt attttgcatg ttacaaacaa taagctaaat
     8341 ttgcatcatc gaaaacagga aaaatcttcc taatccaaat caaaaggttt tagaatttca
     8401 attacttttt tccatcttca ttttcacaca aaacaccatt ttacaaacta tgtacgatat
     8461 acttgaacta aacaaaaagt tgcttcccga attaagggaa attgctaaaa atttgaatat
     8521 taagaaggta gagaacttta agaagcaaga gttaatctac aaaattctcg atcaacaagc
     8581 aataaacgca tcgctttcag ctgacgatga gcggaaaaag gatcaccgaa aacctttccg
     8641 aaagcctgag aaacaggtag aggtaaagag ggaagaaacc caaccggttg cacctaaaaa
     8701 agaggaaccg caagccgcta agcctgacga ggcaacaagt agccaggaag caataaatga
     8761 aaggaataaa cgtaaaagga ttattcgggc aaaagaggtt agaaaggagt tctcagctac
     8821 ccctaccaat agctcaagcg aagaaaagga acgaagcaat aaaaccacca ttaaatttaa
     8881 cgaaaaggcc gttatcctcc ctgagctcga taccgaaaca cattcggaaa ccatactttt
     8941 aagcaatgac gaagaggata cagtaattcc tatggttacc gaagaggctc aagctgaggc
     9001 cttggtttct gaaaaaccgg aacaggaggc acgggcatcg gttaccgaag tggttgaacc
     9061 agtaaaggag gaaaacaatg ctcaacccat tcctagccag gaaaggcgcc ccggtagcga
     9121 gcgatttgat tttgatggca ttgtagccac tacaggtgtt cttgagatta tgcccgatgg
     9181 gtatggtttc ctccgctcat cggactataa ctaccttagc tctcccgacg atgtttacgt
     9241 atcgcaatcg caaatcaagc tttttgggct taaaaccggc gataccgtta ctggcaccat
     9301 tcgcccaccc aaggagggcg aaaagtattt cccattaata aaggtaattg agataaatgg
     9361 tcgcgatcct gaatttatac gcgatagggt tccgttcgat tacttaaccc cacttttccc
     9421 aaacgagaag tttaagctga ttgggaatgg catgaattta ctatcggcac ggattgtcga
     9481 tatgtttgcg cccattggca agggacaacg aggtttaatt gttgcccagc ccaaaaccgg
     9541 taaaaccata ttgctgaaag acattgccaa ctcaatagcg gcaaaccatc cagaggttta
     9601 catgatagtt cttttgattg atgagcgccc cgaggaagta accgatatgg cccgtagcgt
     9661 aaacgccgag gtaatagcat cgacctttga cgagcctgcc gaaaggcacg ttagggtagc
     9721 caatattgtt ctggaaaaag ccaaacgcct tgttgagtgt ggccatgatg tggttatact
     9781 tctcgattca attaccaggt tggcaagggc ttttaacaca gtacagcctg cctccggcaa
     9841 ggtgctatcg ggtggggttg atgccaatgc attgcaaaag cccaagcgtt tctttggcgc
     9901 tgcccgaaat attgagaatg gcggttcgct aaccattctg gcaacagcct taaccgaaac
     9961 cggtagcaag atggacgatg ttatatttga ggagtttaag ggcaccggta acatggagct
    10021 gcagctcgat cgcaagttag cgaataagag ggtattccct gctgtagaca tcatggcatc
    10081 gagtacacgc cgcgaggatc tgctgcttga taaagaagtg ctacagaaga tatggattct
    10141 ccgcaattac cttaccgata tgaactcggt tgaggctatg gaattcctgc gcgataggct
    10201 agtgaaaaca gctactaatg aggaattcct ttggagcatg aacagcgatt tctagtattt
    10261 tttaaggtca ataccgaaag caacatccat ttaaggatgt tgctttttta tttacatggt
    10321 cagctgattg aatgtaaaaa aatgtatttt tgctttactc cacaatattc aaatgacctt
    10381 accaagcgga aactacacca aggaggagtt aagctggttc cgtagtattt ttgatcagta
    10441 ctacgagagt atcaggcatt ttgcctacta taaaaccggc gatgttgatc tttcggacga
    10501 catagtgcag gaaacctttc ttaaactctg ggacatgcgc ggcaaggtca ataacgatac
    10561 ggttaaatcg ttactttaca ccatagctgg caacataatt aagaatcact ataagcacct
    10621 caaggtggtg tatagctttc aggttgcagt taaaggtgat atgacctcaa atccaaatag
    10681 cgccgatgag agaataagaa tggatcagct caataagcaa ttgcaagagg ttttagcatc
    10741 gctacctgat ggacagaggg aagtttttct gatgaaccga attgatgggt taacctatga
    10801 ggaaattgct gcaaggcttg gacttagcgt taaggcaatt gaaaagcgca tgtccgaagc
    10861 actggcagct attcgtcaaa aatttggcta caaaatatga aaagggacag aaatataaat
    10921 agcactgact cagggtttga tagtaatccc attgagttta acatttctgt tcctccatca
    10981 aaccgtacta aagagcaggc gtgggacatg cttatgcaga ccattgaagg taagcatgcg
    11041 gaacccgcaa gggttgtaac ctttcaaccc cattacaaat ggttgcttgc agccgcatca
    11101 gttattatac taatgctggt tggcagctta tttatcctga atactgagtc ccgttttgaa
    11161 acgatgcagg cacaaaccct tgaacataca cttcccgatg gatcggtagt acatctgaat
    11221 gccagctcat ggatttccta taaaaaatca tatggttggt ttggccgaaa tgttagaatg
    11281 gagggagagg cttacttcaa ggtaaagagc ggtaagggaa aattcgtggt taccgataac
    11341 cagaaacaga aggtggttgt aacaggaacg gagtttagcc ttgtaaccag gggtgattcc
    11401 tttgaggtgc gatgctttga gggctcagtg aatgttttga tttcaggttt aaaacccgtt
    11461 ggagttaaaa agggcgagag cgtaactaaa accaatggga tgttaataca ttcaaagcaa
    11521 tccgataagg agttaaccaa acctgtttgg gttacacacg agtttgagtt cgcagaagag
    11581 ccgctttctc aagttttcca gcaaatatca aacttctaca atatacagat tagcgccgag
    11641 ggttttgatc cggcaagccg atacttttct ggaaccattc cagcaggcaa ggcaactgat
    11701 gtacttgatg taatatcaat atctatgggt ttgagctata ctgttaatgc cgattcaacc
    11761 gtttacactt tcaggtaggg aattgggtta ttaagttgta ttaacctaag catgagatat
    11821 aacctgaccc catgagcaga ataaagtatt tacttatatc acttctggtt ggatttgctt
    11881 tacaaatcca acaagctact tatgcccaaa aggttaagat taccgaaagt tttagcaatc
    11941 aatccttaga tcaaatactc tcagctttgt cgggtaagta caacataaaa tttgcctacg
    12001 acccggtagc actcaaccaa atttccttta ccggacagtt taggaacgat tcgcccgata
    12061 aagttttgaa gcagttacta aaagattcag ggtttgagtt tattgcactc aataacgttt
    12121 atgtcattaa aagggcagaa aaaccgattg ttgaagaaaa aaacaaaccc attcagggaa
    12181 ttgtacgcga taggatttcg ggcgaagccc tgccttacgc gtcaataaga gtggtggata
    12241 acgatttaga agctactacc aataccgatg gtttttttac gatacccgat gtaaaatccg
    12301 attcggttag tatagaggtt cggtatcttg gttttgaacc cttacagatg agggtggctc
    12361 gtgtacagta caattcaaaa cctatagttg ttgagctcga tcccaaaaag ttcatcctgg
    12421 ccgatgtgga agtagtgcga aacattacct caatgcttgc taccggcgac cgccccggag
    12481 agatggtttt tgacacaaaa aaggtaggcg atataccagg aatctcagga attgatattt
    12541 tggcaccact tcagctcctt ccgggagtag atggtactac tgaatcgctt tcagggctga
    12601 tggttcgcca ttcgccagcc gataaaaacc tgatctcgta cgatggcttt acaatttatc
    12661 acattaacca tctttttggc gcattctcaa gcttgaacag taaggccata aaggatgtaa
    12721 ggctttaccg cggcggtttc gattcgaggt ggggtggtcg tgcatcgtca gtaatagaaa
    12781 taacaggtaa aaccggcaac gagaatagtg taagtgttga tgctggcacc gaccagcttg
    12841 ctggcgattt cacatttgaa ggtccgttgg ggaaacatgc ctcctttata gtgtcggcgc
    12901 gtagatccta taccgatttt taccgttccc cacagtttct gaatcttttt gagagtgtgg
    12961 cttcggatct tatgctttcc aagcagaact ttaccgcgtt tggttccgat cccgacgccc
    13021 cctcgtatct tttttacgac gcaaacgcaa aggttagcgt gaaaccctca aacaacgata
    13081 ttttatcggt aagtgggtat gtaggacgcg ataggctcaa tttcctgcaa atggtatcat
    13141 cgccttttat taatgaaaat gctctttggg ggaatagtgg tgctggcgct cgctgggcta
    13201 agcagtggaa tagctggtta taccaaaatt ttaccgttgg gatttctgaa tttacaagca
    13261 actatcaaca ttacgacagc acattaaaac gccgtggaat acagccggtt tacgacacca
    13321 ttgtccgaaa ctatgcaacc gaaacgggat taaacgatct ctctgtaaac ctgaatgccc
    13381 aggtaactct ttcgaaccaa atcatactag agctgggata ccagagtaat ttagtgaatt
    13441 ctaaatcatc tgagaattat tatcatgctg taaactccct tcctgttgcc gataccctaa
    13501 gacagtacga ttttaattca agggttaata ctggttggct acaatttaac tttaaaacca
    13561 atgggttgaa atcacttgtc attggggggc gagtgagtca acataacctt accgaccaat
    13621 actacgtaga gccgcgtgtc caaatggtga taagcccaac atcaaagctc gatttaaagt
    13681 ttgctgcagg cttgtataac caatttataa accgtattta cctaactggc aacggggttc
    13741 gctatttgtg ggtagcttcc gacgaaaaga cctttccggt agtaaagtca aagcactttg
    13801 ttaccggttt gaactacaca agcaatggat taacattaga tattgaagga tatgcaaagc
    13861 aaaccgaggg cttatcgtac gttcaaactg tgatccggcg aacctcaacc aaccgatttg
    13921 tagagcaatc caaaattttt tatgttgata gtcgttcatt gggggttgat gttttagtcc
    13981 gaaaaacatg gcagaatgcc gagggttggg tatcctattc gcttagtaag gcttttaatc
    14041 agtcatcaac cctgaacggg ggcgatgagt actatgccct cgacgaccat ttgcatgagt
    14101 taaagttagt tggtttatac aagtggcgaa aatggagagt tgttggaact tggatttttg
    14161 gtactcctaa accctgggat gagctcttgc taactccaag ccttaccctt tcacctgatt
    14221 acgaaaaaaa ctcgagcagg ttagagccgt accacaggtt agatgcaggg ttaagctact
    14281 tggcaaaggt ttcagttagt ggtgaacttg aggttggtgt aaaagtgttt aacctttata
    14341 atagggataa tatgcttgcg cgcccctaca cgcttaccga tacacctatt tccgattacc
    14401 tacagggcaa atcaatcatt gagtatcacg atatttacgg ctacagcatt acccccacat
    14461 tcttttttaa tatcaagttt taataagtta agtttaaaac tatttctaac ctgtagattt
    14521 atttcaggac tagacaacct ttatctttta tcctttaacc tttatccttt aacctttaaa
    14581 ctttatccct atccttcacc attctcatta tcagcttatc gggtattgcc actaacctgt
    14641 acaccgattg gtaaaaacac tgattgttat gcatacatgt tacctgtgca atatatgttt
    14701 tattggcttt ttctctgtcg aacctaatat tgtactcaat tgtaccttca ggattaagtt
    14761 cagcgtgctg atttacccta aatatttcaa gtgtttggta aaagggtgat acctgatcgg
    14821 ggttgatggc tttgtttagc aaccggttaa tttccgtttc tacctcggtt atcggctctc
    14881 ggatggcttt aaaaagagca gcgctagcgt atgctattgt aaaaaggaaa tcggaaaggg
    14941 tgttgttcaa gctgttggtt agctccctaa aggtttccag ctgcgtttta tggaaaggca
    15001 tagctcttac aatgccatct aaatcgagta ccttgggctc gatatttttc agaactactg
    15061 acctctcgtt ctttgtggaa aacatatcgt gcccgtttat ggcatatagg taattttgat
    15121 cgcctttggt ttcaaaacct aagtcaatag gctcgttggt acaaattacg ttgccaaaaa
    15181 ttagtttgta gtaattaaaa tactcgtcgg ttttcctgtg aagcaaattg acaattgttg
    15241 aaaataaaag catgccggga acaacaactc ccttgttctg gttgtacatg aacactggat
    15301 catgaattgg gttatcatca acggtagctt tgcaaaagtt tgcaacaacg gaatcggtaa
    15361 acaatacttt attttccata tcgggtaaaa aaacagatgc acaaatttaa aaaatcaaat
    15421 taaatcaaac aaaaatcttc taaaaattta ttcttcgttt ggtttactaa agccccatta
    15481 aaatgattgg ctaaagcaaa aggggcatta tcaaattttc aagtaatacg gtagggtatt
    15541 ttttaaggta gatgtattag tagcaaaaca aatacagaac aagttcaatt attttttttg
    15601 tggatacggt agggtgtttt ggaatagggt tgtattaata gcaaaaggat aacaaaatga
    15661 ctcgggacgg gaaaatagaa aggctacctg aaaacattga aaggctactt gatagctaca
    15721 atgtgcctgc agggaaaatg cgttccgaag cctgggaagc gatctttgaa aaaattgacc
    15781 ataaaaactt tgaggaagac tcaaagggaa gagtaataca attgacatta actcgtgttg
    15841 cagtagcagc atcagtttta gttgccctaa tgcttacctc atacatctat ttgtatagga
    15901 tgggaaatgt gcacgtgcag ctagcgcgtg gtgaacacgg tgtggtttac ctgcccgata
    15961 gtagcagggt tactctgaat gccgaatcga aaattaccta taacaagaat cgatggtttt
    16021 tcgataggag tgtatcgtta actggcgagg cgttgtttga ggttagcaag gggcgtcgtt
    16081 ttaaagtggt aacaactggt gcaaccacaa cagttcttgg tactgtattc aatgtgttta
    16141 gccgcaatgg actagttaag gtaacctgtt ttgaaggcaa agtaaaagta gttgccacaa
    16201 acagtaagtc gcaaacattg ctaactaaag gtatggaggc aaaaacaatg ggatatgctt
    16261 tagatgtaaa taaaacacaa acaacaaaag cggagtctaa acccgcatgg actaacaacg
    16321 agttcttttt tcagaatgct cctttaagtg ctgtgattga ggaattagaa cgtcaattca
    16381 atgtggatat tttccttaat actgacacaa gtaggtttta taccggctac ttcaaacgta
    16441 caaacctgga cgaagcttta aacctggtgt gcataccgct tgatttaaag tgggattaca
    16501 gtggtgaaat aatagtaatt acgagcaaat aaatgatttt aaaaaccaat tgtctaactt
    16561 aaattttaca gctatgaaac gtacaagttt aatttttgca gcaggcctta tgttaatggc
    16621 agcttttaca ctctcctcat gccaaaagga cgaggaagta gattcagtgg ttaccctggc
    16681 tagcgaagac gccgatgtgg atacctacta cgatgatgta atgaccgaag tggatgaaat
    16741 tacacttgct ggtgattcga aggactcgca gctgatgtat gcaacactcg gtggtcaagg
    16801 tacacgtact cgtaaaacat cctgggacgg aaattgccgc atcgactctc taacttatga
    16861 gaactttgtg aatgctaact cccgttacga gagagtaaag aacgggttaa ttgtaatccg
    16921 tacctgtggt cagtataatg gtgctgaatt tgagcgtact gttaccttca aaaattttac
    16981 cattaacggt aacctgattg aaggaacaaa acaaatccgc aaaactgctg agcatgagta
    17041 tcagattaca cttactaatg gtaaaattac ctttaccgat ggtaccacct acacccgtac
    17101 tgtagagcgc acccgtactt gggttgaagg ttatgatact ccgttctaca tttgggatga
    17161 tgtttacacc ctggaaggtg tagccacagg cgtaaaccgt agaggttaca cttacactca
    17221 ccaaattacc agtgcactca tgttcaaact atcgtgcagg tggttggttc aaggtgtgat
    17281 tgatttgact gtaggcgata agcaggccac tattgattac ggtaatggtg attgcgataa
    17341 catagcctac gtaacagtaa atggcaaaac ctacgaggta aagctgaggg gtggcaagta
    17401 gtaaaataag aactagaaag ataaaaagga ggggaaaccc tcctttttta tttttattgc
    17461 ctttatgtga attttaaatt agttttgggg ttcagttaaa atctcctttt gctcataatt
    17521 tgacagtccg tgctcattgt taaatatcaa cataaattaa catattgata tatgaaaata
    17581 tattattttt gtagccgaaa aaaatgaaca aaatgagcac tatagttgtt gatgaagcac
    17641 gatgcccgca gaaccatcct tgtccggtgg ttcgttactg cccaacaggt gcaattaaac
    17701 agcgtagctg gaatagcgca ccagagattg ataaggaaaa ctgcaagaat tgcggtttgt
    17761 gcgttaggat ttgtgggtac ggtgcattta cctttgccga taatggcagg tagtaaaggg
    17821 agggtataac ccctgccctt tgattatgaa aatattaaaa tagagatagc tatgagaaaa
    17881 attactgctt ttttcccggt tcttttggtt gcagcctttg cgctaggagc atgtaacgga
    17941 accgccgcca agcaaaacga aggtgaaccc gttactgcat taaaaccagc taaagagaaa
    18001 tatgccattg atattacccg tgactttttc ctgaccaaaa ttttggatta caccaaggga
    18061 caggaaaata tgaaatacct tggcgataag cctgccattg tcgatttctg ggcaagctgg
    18121 tgcggccctt gccgtattgc gggtccaatc ctggaggaac tggccaagga gtacgatggc
    18181 aaaatttatg tttacaaggt gaacacccaa aacgagcagc agatctctgc tgaacttggt
    18241 atacagagca taccaacatt tatattcttt cccatggacg gaaagccttt tgctacctca
    18301 ggtatagctc aaacccctga ggaaactaaa aaaatgttca aggagattat tgagaaagaa
    18361 ctactcaaga aaaagtaatt agtactcccg tcataactgt ttttggttgg ttggccggct
    18421 ttatgccggc ttatttgttc ttagagcagt gggctggtgt tttgttttag ctcctcaatg
    18481 ctgtaaatgc ggctaaactc acagtcggag agtactatgt attcgtaacg ttggttgcgg
    18541 agttgaggtt caaacccggc atcaacaatg gctttctgaa taccataggc atccatcttg
    18601 tacttggctc cagccgatga tacaacattc tcctcaatca ttattgagcc aaaatcgtta
    18661 gccccagcgt gcaagcacag ctgagcggta tcgataccaa cagtaagcca ggatgcttgt
    18721 atgttcggaa tgttattcaa catgatcctg catagggcta tagtcctaac atattcctca
    18781 gtggttaccg tatttagcac gccctcgcgc tgtagtttgg tgccctcatc ctgaaaggtc
    18841 caaacaataa agttcagaaa tccgggggcg tcggcaggct tttgcgcttg gaggttacgg
    18901 agtttaatca aatgctccac acgctcctcc agggtttcaa catgcccaat catcatagta
    18961 gcagaggtaa caagacccat ttggtgcgct tgagccataa catcgagcca ggcctggcta
    19021 tcgggtttgc ccggcgatat ttgctttcgt actctgtcca caagaatttc ggcaccagca
    19081 ccgggtaaac tatcgaggcc tgcctccaca agctcttcaa gaacttgccg gtaggtcata
    19141 ccttcttttt tagctataaa ggcaatttca ggaggcccta gggcgtgaag tttgatgttg
    19201 ggaaacattg actttagttc cttgaatagg ctgcaataaa actcaacacc aagcctggga
    19261 tgaagtccac cctgtagtag aagctggtcg cctccgtagc gctcaagctc ttcaatcttt
    19321 actttatact ctgcaattga cgtgataaag gcgctatcgg actgaagccc acagtggaag
    19381 ttgcaaaact tgcatcccga tatacataca tttgtgatgt ttacgttgcg gtcaatctgc
    19441 caggtaacct tgtggcctgg aacatgaatt ttgcgaagct cgtttgcaac atacatcaaa
    19501 tcgggcgttg atgcctgtgt gtaaatataa aggccctctt ccaaggtgag ggattcaagg
    19561 tttattgcct tttttaaaat atctttaact tgcattcttc aatgttgatt gggcaaaggt
    19621 attaaaagta acaaaaacat tcctagtaaa aacaacaagg tgttaactat gtttacagct
    19681 tttttttact tttgcatatt tacgagccat ttaaaatact acaaggtatg agtaatgaaa
    19741 agattagggt aggaatcacc cagggtgata ttaacggaat aggctatgag gtaattatta
    19801 aaacattgat ggaccctcgt atggacgaga tgtgcacatc aattgtttac ggatcaccca
    19861 aggttgctgc ctatcatcgc aaagcgttga acattgaaaa cttcagcttt aaccaaataa
    19921 aggatgcctc agaggcaaac cccaaacgcc ccaatattgt aaactgcatg gacgaaaacg
    19981 ttcgggtgga gttgggtaaa tcaactgagt atggcggaca gggtgcgttg ctagcattac
    20041 aggctgctat agcagactta aaagctggta aaattgacgt actggttacc gcacccatca
    20101 ataaggctaa catacaaagt caaagcttta gctttccggg gcataccgag ttcttggcca
    20161 atgagtttgg tgttaaaagc gcgctaatgc tcatggtaag cgataccatg aaggtaggtg
    20221 ttgttacggg tcatatccca attgctgatg ttccaaaaaa cattactgtt gatgccatac
    20281 tcaacaagtt acgcttgctt aacgagacac taattaagga ttttgcaatc cgccggcctc
    20341 gtattgctgt acttggcttg aacccccatg ctggcgatag cggtgttatt ggcaacgagg
    20401 aggaaacgat tatattacca gcaataaagc aggccaacga ggagggaata gtagccatgg
    20461 gaccctatcc ggccgatggt ttctttggct ccgataggtt cacccgtttc gatgcaattc
    20521 tggccatgta ccacgatcaa ggcttatcgc cttttaaggt gataaacttc gatacaggag
    20581 taaactatac tgctggttta cccattgttc gcacctcgcc cgatcatgga acggcatacg
    20641 atttggccgg acataacctg gctaacccga actcattccg ccaggcggtt taccttgcca
    20701 ttgatatttt ccgtaaccga cagctgcata aggagcttac ctcaaacccg ttgcagtcag
    20761 ttgacccaga aacggcataa aaaagttgat ttatgtacga gtacattcag ggttcccttg
    20821 ccgagctgac accaacccat gcaattgttg atgctggtgg agttgggtat tttgtaaata
    20881 tttcgttaca aacatactct cagctggagg gtaaaacaca ggttaagctc tggcttcatc
    20941 aaattatccg cgaggatgct catttgctct acggtttcac cgatgtacgc gaaagggaga
    21001 ttttccgctt gcttatttca gtttctggag ttggtgctag tacggcaagg cttattcttt
    21061 cgtcgcttac taccgatgag attcaggcag ccattaccac ggaaaatgtt aacctcataa
    21121 aacaggttaa gggaattggc cttaaaacgg cacaacgcat agttgttgat cttaaggata
    21181 aggtttccat aggggaggct acggtttcac aaattttatc aacatcaaac aatactttaa
    21241 agagcgaagc gttatctgca ttggtgatgc tcggctttgc ccgttcagct gccgataagg
    21301 taattgacca ggttctgaag gaaatggggc aaatgccagt tgaagcgctc attaagcatg
    21361 cgctcaaacg cttgtagtaa cagtaaaatg ctttgtaagt tgagggtaaa acacttatct
    21421 catacacata ctatccgaat aattggaata cttactctcg taaccattac cattacgaga
    21481 gctttttctg ttcccattcc acaacaggat acattaaatt tgcctttccc tattaaacaa
    21541 cccggtatat ggtcaattga cacgcagtat tcaccattat acctttcaag cccatcaaac
    21601 ataagcgagg aggttgtgtt tgatccaatc aacaaccagt acatcattta cagaaaggtt
    21661 ggaaatacaa ccattgaaac accacgtgtg ctatcggccg atgagtaccg gtcgtatcgt
    21721 gtggagaagg ccatgcgcga gtactggcga caaaagcaaa ccggtgaatt tgtggggaaa
    21781 ggcgatggca ttctgccccg tattcaggta ggtggtgaaa cattcgaccg aatttttggg
    21841 agcaacacca tagaaattat accacagggt aatgccgagc tggtgtttgg agtatcaagc
    21901 gctaaaaccg ataacccagc attaccaatc gatcagcgac ggaacacaac cttcgacttt
    21961 cagtcgaaaa tccagatgaa cgtttccggt aagattggtg agaagctaaa aatggaggtg
    22021 aattacaaca ccgaggcgac ctttgacttt gagaataatg ttaaaataga gtacaacgga
    22081 tttgaggatg aaatcattca gcgcattgag gctggtaacg tttcgttgcc tttacccgga
    22141 actctaataa ccgggagtca gagccttttt ggtattaaaa ctcagcttaa gttcggaaag
    22201 ttaaacgtta ctagcgtagt atccaaacag aatggccaaa cacaggtggt tgagattaag
    22261 ggtggtgcgc aaacgcgcga ttttcagctt aaagccgatg agtacgatgc caataggcat
    22321 tttttccttt ctcactactt tagggaaaac tacaatcgtg ccctgatgaa tttacccatt
    22381 attaactcag gggtacaaat caccaaaatt gaggtgtggg taacaaacaa acaggcaaac
    22441 tttgagaact cccgcaatat aattgctttt gccgatttgg gcgaggccca gaacaacatc
    22501 tttgcttcca atatttttgc ccaaacggga agtggcccgg caagcaacgg tttaaacaac
    22561 ctgtacgagc ttatgaccac aacctacgcc ggtattcggg atgttgccga agtaagcaat
    22621 gtattgttgc ccttggaatc gcagggtttt accggtgggc gcgattatga gaagattgag
    22681 tatgcccgta agctatcacc caatgagttc accataaatc aggcgttggg gtatatctcg
    22741 ctatcgtcgg cgctcaatag cgatgaggta cttgcggttg catatgagta cacatacaat
    22801 ggccaaacct acaaggttgg tgaattctct accgatgggg tagaggctcc taaagcctta
    22861 atccttaaac tcctaaaggg aacaaacctt agccctaaaa tgcccacatg gaggttgatg
    22921 atgaaaaaca tctactccat gaatgcctat cagattagca aagatgattt caggctcgat
    22981 attttttacc aggacgatgc aaccggaacc ccgattaact atatcaagga agggaatatt
    23041 gccgaagttc cgctaatcag agtacttaat cttgataacc tgaacaacca gctcgatgca
    23101 ttccccgatg gagtgtttga ctttattgag ggtgtaacca ttaatcccgc taacggcaaa
    23161 attatttttc ctgttttgga accatttgga cgcgatttgg catcgcgtat aggcgatcct
    23221 actattgccc agaagtatat tttccaagag ctttacgatt catcgttaac caaagcacga
    23281 caggtagccg ataagaacaa gttcctaata gggggttcct acaagtcggc cggaggatcg
    23341 gaaatctttc ttaatgcgcc caatattcct aaaggtgccg tggttgtaac tgcaggtggt
    23401 gtaaaactgc aggaggatgt tgattttatt gttgattaca atctgggtac tgtacgaatt
    23461 atcaaccagg gacttcttga atccggtact cccataaagg ttgcagtgga aagcaacgcc
    23521 ttgttcagcc tacaaaccaa aaccttggtt ggaacccact ttgattaccg tttctccgat
    23581 aactttaatg ttggggcaac cataatgaac ctcacggagc gccccctaac ccaaaaggta
    23641 agttttggaa acgaggcagt atcgaataca atatggggat ttaatacctc gtaccaaacc
    23701 gagtccaagt tcctgactaa aatggtcgac tttcttccat tcattcaaac caaggaaaaa
    23761 tcaacaataa catttgacgc tgagtttgca cagctaattc ctgggcactc gcgtgccatt
    23821 ggtaaggccg gtacagtata tatcgatgat tttgagggga gcagcacatc gcttgatatt
    23881 cgctcatggg tagaatggaa gttagcaagt actccccagg ggcaacccga tatgttcccg
    23941 gaagcctcgt tctccaacga tttacggtat ggatacaacc gcgcccattt ggcttggtac
    24001 tacatcgatc cgctattttt gaggaacacg tcgcttacgc catcgtatct ccgtaataat
    24061 aaggagctgc agggtaacca ttttgttcgc gaaatatttg agcgcgagct ttttccaaac
    24121 aagaacacgc cacaggggca accaaccaat atctccgttc ttaaccttgc attctatcct
    24181 actgaacgtg gccagtataa ctttaatact actgaacttg atgcaaacgg aaggttaata
    24241 gatccgcagc gccgttgggg tggtataatg cgcaacctga gcaccactga ctttgaagca
    24301 tcaaatattg agtatattga gttttggttg atggatccgt ttgtgtacga cacgctcagt
    24361 agtggcggcg atttttacat caaccttggt aacatctccg aggatatttt aagggatggc
    24421 cgaaaatcgt ttgagaatgg actacccacc tcatcggagg agctttccaa aaccgatcaa
    24481 accgcctggg gtagggttcc tactaagcag tatctgcctg ttggtttcga taacgaccaa
    24541 gctaagcgcc cctaccagga tattggatta gatggactct cctcgtcggt cgatttggat
    24601 ggcgatggga tactcgatga gcagttcttc ttttcggagt atataaacca gctcaggaat
    24661 aaaataacca atagcgatgt gctcgccgga tttgaaagcg atccggcagg cgataacttc
    24721 aggcattacc ttagcggggt ttacgaccaa cagcgtgcca ccattctcga ccgatataag
    24781 tactttaacg gaaccgagaa taattcgcca gttgcaactg gaactacctc gcagcagagc
    24841 tactcaaccc ccgatgtgga ggagctggga accgataata ccatgaatga aaatgaggct
    24901 tactatcagt atcgcatttc acttcgcccg caggacatgg aggttggtaa gaattttata
    24961 actaacgttg tgacaggcga taaggataaa attttaaata aagcagtaaa gtggtatcaa
    25021 tttaaggttc ccattgcaga atacgagaag gtggttggcc caattgagga tttcaagtca
    25081 atccgtttca tgaggatgtt cctgcgcggt ttccaggata cggttatact acgttttgct
    25141 acgctgcatc tggttcgtag cgagtggcgt aaatacaatt actcacttat tcaaggccag
    25201 gagggtatgc cagttggcga tttaccttca ggcagcttat cgatagcaac cgtaaatatt
    25261 gaggagaacg acaaacgaac acccgttaac tacgtattgc caccaggcgt ggatagggtt
    25321 attgacccaa gtcaacccca gcttatccag cttaacgaac aggccatgga gtacagggta
    25381 accgatttgg ccgatggcga tgcccgtgca gcgtttaaga atgttaacat cgatatcagg
    25441 cagtacaaac gactcaaaat ggatgttcat gccgaggcaa ttgagggata tccattaaac
    25501 gactacgagg taaatgcttt catccgtata ggtaccgact ataacactaa ctactacgag
    25561 tacgaaatac cattaaagct gactccttac gggttttact acaatagccg ggaacgcgat
    25621 cgcgaggcgg tatggccaag ggagaatgta attgatatcg acctcgataa gctggttgat
    25681 gttaaagtag cccgaaacaa tgccatgcaa aaaccagggt cgcttatcac cctgaatact
    25741 gtgtatgttg ttcaaagcgg gaacagtaag ctaaaagtta ccggaaatcc gaaccttagc
    25801 aacgttaagg taataatgat aggaattagg aaccctgccg atgatagaat gcctaaatcg
    25861 gttatagcat ggttcaacga gttaaggctt actaacttta acgatcaaag tggatgggca
    25921 gccaatgccc gcttatcggc aaaacttgcc gatttgggaa tggttaccgt agctggcacc
    25981 accattacac ccggttttgg ttccattgag aaaaaggtta acgagcggag caaggagaca
    26041 gtttaccagt acgatatcaa ctcaaccctt gagctgggta agtttttccc cgcctcgttc
    26101 aacattaaaa ttccaatgtt cattggattc ggcgaatcga tggcaaaccc ccagtacaac
    26161 ccgcttgacc ccgatatact tctcgacgat gccctacgta acgcatcgga acgcgaaaga
    26221 gattccatta aacggattgc ccaggattat actcgcaggc gaagtatcaa cttcaccaat
    26281 gtaaagattg ataaaatggg ctctaagccc cgcctatggg atcccgccaa tatttcggta
    26341 agctactctt acagcgagat tttctcccgt aacgttagaa ttgatcatag gattcagcgc
    26401 aatattcggg gtgcatttac ctataattat aatcgacagc ccaagtatat atccccattt
    26461 agcaagataa agtggttaaa ttcaagctat ttggctttaa tacgcgattt taactttaac
    26521 ctattcccaa cccagctctc gttccgtacc gatctgaata ggaattactt tgaacagcaa
    26581 ttccgaaata ttcagaaccc caatcaggta tttttaccca cattttcaaa ggattttatg
    26641 tggaatagga actactccct gaactgggat attacccaat cggttaagtt tgattttgtg
    26701 gctacagcta ctgctcgtat cgatgaacct gagggcatgg tggacaggta tagggatagg
    26761 gacaggtatg agcagtggaa ggactcggtt tggcagaata tccgtagggg tggacgaaac
    26821 accatgtaca accatcagtt taacataacc tattcccttc cgttaagcaa aattccatta
    26881 ctcaactgga ttagcattac atcgcgttac accggaatat accgttggca ggccgggcca
    26941 attttggccg acacttcacg gttcgatccg ggaaatacta ttcagaactc aaacaccatt
    27001 cagctgaatg ggcaggtaaa cattaatacc ttatttaata aggtaggata ccttaaaagg
    27061 gttaaccagc agtttgacca gcgggcaagg ggggctgcac cccaaacccg aacggttaag
    27121 tatgaggagg aaaaggtaaa ccttagggca aatgcaacta aggttattac ccacaagcta
    27181 aaaacggaaa aggttaccgt aaaggttttc gaccccaatg gcaaggagat aaaggtaagt
    27241 tcacaggtac gtggcgacac caaggtagcc attaaggccg atgccgatta taagaactgc
    27301 aaggttgtgg ttgaaggcaa agtccctgaa aagattaacc ccgccaggtt tgcccttgaa
    27361 accacactcc gacttttgat gagcgtaaaa agcctaggag catcgtacac ggaaacaaac
    27421 ggaacactac tacctggtta taaacctggg gtaaactact tgggaactga gagctttaat
    27481 ggtatgcttg cccccggctg ggaatttgta gctggttggc aggaccccga ctttgcttgg
    27541 aaggctgcta ctaatggttg gctcacccgc gacacatcgt ttaacacacc agtagttttt
    27601 actcgcaatg agaatcttaa cttcagagca acactggaac ctttaccggg cttccgtttc
    27661 gatgttagcg ctacgcgaac actgggtaaa agcaatagca aaatttttaa atccgatggt
    27721 ttgggttctt acagggtgct aagtccgctc acaacaggaa atttcagtat tagcgtgctt
    27781 accataggta ctgcattcga gggtggtaaa cggactggaa gctccaaggc ttttgaaagg
    27841 atgaagcaaa accgattgga aattgcctat aggctccgcg ataggagggt tgcaaatcca
    27901 tcgcagggct atgaccccaa tgtgatagat cctgttacag gatttcccga tggctatggg
    27961 cctttatcgc aggatgttct cagggcctcg tttttggccg cttacaccaa tacatcccca
    28021 agcaaggtct cgctaagcga ttttcccctt ataccaatgc ctaactggca gctatcgtac
    28081 gatggtttgg ccaagatgaa accttttaag cgttggttta agtcaattac tatcaatcat
    28141 gggtatcgat cgctctatac cattggggct tttacaagca atcttaccta tttacccgag
    28201 gatgatgggt ttagctacgt tcgtaacatt aacggcgatt ttgtcccgca gaacgagatc
    28261 actaacgttg ctataaacga gcaatttacc ccattggttg ggctcgattt gggatggaac
    28321 aataacctta ccaacaggat cgagattaag aatagccgtt cattagccat gagctttgcc
    28381 aataaccagc ttaccgagat ttatggcaac gagtttatcg taggagctgg ttaccggttt
    28441 gaggatttac ctcttatttt ccaggccgag gatggtggta aaaaggcaat caagagcgat
    28501 ttacgattaa ctgtggattt ttcgctacgc gataatacta ccattcttcg aaagttggtt
    28561 gaggattcga acgatattat tgcaggtcaa ctgattagca ctataaaaac agctgctgac
    28621 taccggataa acgagaaggt aaccattcga ctgttctttg accgcaccct caataaccct
    28681 aaggtttcaa gaacattccg aacaaccaat accagctttg gatttagcgt taggtttgag
    28741 ttagttaact aatgtgctga ataacatgca gcactaatta aaaaaaccga taggattgga
    28801 tatacaatct taaaattcac tacttttgaa cagcaaaatc tttagtgtaa tactattaac
    28861 tgataatact atgaatattc catcaaatct aaagtacaca aaggatcacg agtggatcaa
    28921 ggacgaaggc aatggcatag ctaccgttgg cgtaaccgat tttgcccaaa cacagcttgg
    28981 cgacattgtt ttcattgaga ttgaaactca gggtgaaacc cttgccaggg aggaggtttt
    29041 tggcaccatt gaggctgtta aaaccgtttc cgatatgttt atgcctgtta gtggagaggt
    29101 agttgaagtt aaccctaagg ttactgaaca acccgatatc gttaacaagg atccctatgg
    29161 tgaaggttgg atgattaaaa tcaaaatgtc caatcctgct gagatgaacg atttgctcaa
    29221 tgccgagcag tacaaaggaa ttatcaacga gtaaaaaaaa gtaaaccccg ctcaagcggg
    29281 gttttttatt gctattcctt ttgtaaagca ttccagccct gagcagtaat ccttatgtct
    29341 tcacccgatg gtggtatcat gtatgcacca aggtgtggct caacaatatg ccctatcact
    29401 ctgacatttt ccatgctatc aactacctgg tattccgata gcggaaccgt gaacaaaagc
    29461 tcgtagtcct cgcctccgtt cagtgcagca gtaaacatat caatattaaa ttcctcggcc
    29521 attcgtgcgg tttgctcatc aataggaatt ttgtccatgt atatcctgca tcccacgttg
    29581 gagctcttgc aaatatgcag cagctccgac gacagcccat cggaaacatc tatcatagat
    29641 gtaggtttaa cccctgcctt tcgtaggttc tcaattatat tccttcgggc ttcgggtttc
    29701 aggaatcgct caagaatgta gtcgtaacct tccagctttg gcttggctgc cttatttgaa
    29761 aggaagattc tcttttcgcg ttccagcagt tgaagcccca tgtaagcccc tcctaaatta
    29821 ccggttacgc aaattaagtc atttaccttg gcaccactcc ggtaaacaat ttcatcctcc
    29881 tttgcgctgc caatagcagt aacacttata gtcattccag tcatcgacga cgatgtatca
    29941 ccacctacca ggtcaacatt gtagctatcg caggcaagct taattccgct gtatagctca
    30001 tcaagatcct ccacacagaa acgcttcgaa acgcctatgg aaaccgttac ctgtttaggg
    30061 attgcgttca ttgcgtaaac atcgctgagg ttaaccacaa tagctttgta gcctaagtgt
    30121 ttaaggggtg tgtaggttaa gtcgaaatga attccttcaa gtagcaaatc ggtagtaacc
    30181 acaaggcatt caccttttcc gcatgatatt accgcagcat catcgcccac accttttagt
    30241 gtctcttgat gttttggggc aaacggctca gttaacctat caatcagctt aaactcaccc
    30301 agttcgtaaa gctctgttct tttctgcgtt tcgctcatct ttttaatttt tctgcaaacc
    30361 tacatcaaaa tagaataacc aaaatgcagt tacattcttt tttggtagtt ttggggcaaa
    30421 attcagaaaa tttatgctta aaaagatttt acccctttta gttatttctt tactcacagt
    30481 gttcataatt gactatttca taggtaccgc ctacattaaa ctatacagcc ctgaaaagga
    30541 tcaaaaacca ttggtggagg atgtgcaacc catactggag tatggaatac caatcgattc
    30601 gttttatgtt actgaataca ccgttaggag taaccaaaca ctagggaaaa tacttttcag
    30661 cttaaaccta actggaaatg ttaatgagtt tctgctctcg cttacgcccg atatttttga
    30721 tgtgaaaaag gttagggcgg gggcacggta taaggttttc gcccggaagg attcccttca
    30781 gactcctgct tatcttgttt atgaggtttc gcctgttgaa tatttcgttt ttaaccttac
    30841 cgattccctt tacgtttacc cctttaaaaa agagattgta cctgttagga aaatttccga
    30901 ggctgaaatc aaatcgtcgc tttgggtcga tatgaaggca aatggtctaa atcccatgct
    30961 tgcccttgag ctttccgata tttttgcttg gacagtcgat ttctttggcc tttttaaggg
    31021 tgataagttc agggtgatgt acgatgaact ttatgttgat agcacatcaa ttgggattgg
    31081 gactgtttat gctgcatggt tcgagcatcg tggcgaaaaa ttttacgcgt tcaggtttga
    31141 acaggactcc atagccagct actgggacga aaaagggaat tcacttcgta agtcgttcct
    31201 caaagctccg ctccgctttt cacgtatttc aagccgtttt agcgggaacc gcttccaccc
    31261 tgtgctaaaa atttatcgcc ctcattccgg tgtggattat gctgctcctg aaggaacacc
    31321 tgtaatggcc ctaggcgatg gtgttgtaat tgatatgggt tacgataagg ccgcaggcaa
    31381 ttacattaaa attcgccata acagcgtata tacaactggc tacaatcatt tttcacgatt
    31441 tgccaaggga attgcaaggg gtgctcgtgt aaaacagggc gatattatag ggtatgttgg
    31501 tcgtacaggc tatgccactg gcccacacct cgacatgcgt gtttggatga atggctcacc
    31561 cattgaccct ctaaaaatga aatcacctcc ggtggagccc gttaaacagg aaaatcttga
    31621 aactttcagg aatgtagtag ccaaactctc agcacaactc gatagcataa cacccggaag
    31681 cagaatctac tatgcagttc aaaaagagta aaccatgctt ttgattgata ctcactcaca
    31741 tatattctcg gacgagttcg ataccgatcg tgaagctgtt cttatgcgtg ctgccgagat
    31801 tggtgtgcat tatcacattt tgccaaatat cgattcctcc tctaccaacc gtttgctcga
    31861 tacctgcaat cgctttccgc aatgcttccc tttaatcggt ttgcatccta cctctgtccg
    31921 gaacgggttt gaaagggaaa tctatcacgt tgaggagcag ttggccaaga ccaggttctg
    31981 gggcattggg gaaataggaa ttgatttgta ctgggataaa acatacttta tacagcaaca
    32041 ggaggcattc aggcatcagc tcaggctggc caaaaagctc aaccttccgg ttgtaattca
    32101 ttcccgcaat tctttcaggg agatctttga tattgtcgat caggaaattg acaacaccct
    32161 aaagggagtt tttcacagct ttactggaac tattgacgat tataaacaca ttgacgaata
    32221 caaaaccttt ttggtgggga ttggtggtat tgtaacctac aagaatggtg gggttaacgg
    32281 tttagtaaac cagttggatc caaacaaaat tttattggaa actgattccc cttacctatc
    32341 gccagtgcct gtcaggggca agcgaaacga gagctataac cttatctaca ttgctgacaa
    32401 ggtagccgag ttactcggag ttactgtgga gcaggttgcg aaacaaacca cccaaaatgc
    32461 cgttaaactc ttcaaaatta agatagatga aatcgggtaa tacatcaatt ctaattattt
    32521 acactggtgg aacaattggg atgaagcaaa accctgaaac cggggcattg gctcccttta
    32581 attttgagca aattgaacag gaggttcctg aactcaaaaa atttggcttc aaccttagca
    32641 catactcctt tgaaccccca atcgattcat cggatatcaa tcccgatttc tggaggaagt
    32701 tagcggcaat aattggggat aattaccaaa gctacgatgg ttttgtgatt ctccatggaa
    32761 ccgacaccat gtcgtttaca gcttctgctc tgagctttat gctcgagggg ctgtcaaaac
    32821 ccgttgttct taccggttct cagctaccca taggcatgtt gcgaaccgat gggaaggaga
    32881 accttatcag tgccattgag attgccgcct cccttcaaaa cgggcaggca atggtaccgg
    32941 aggtaaccat tttttttgag aataggctat accgtggaaa cagaacaacc aagcataact
    33001 ccgaacattt taatgctttc cgttccgaca actatccccc ccttgccgag gcaggtatat
    33061 caataaagta caattaccct tttattcatt accctactca ggaacagcac cttattgtcc
    33121 acacacagct cgatagctct attgctgtgc ttaaactttt tccgggaata tcggaaaatg
    33181 ttgtggaatc cattttaacc accaaagggg taaaagcggt tatactggaa acctacggct
    33241 cgggcaacgc accaatgcag aagtggtttt taaagaaact ggaatatgct ataaacagta
    33301 atatagttgt gttaaacgtt acccaatgca aggctggcgg ggttgacatg gataagtatg
    33361 agaatggcct tatgctaaaa cggttgggtg tagtaagtgg ccacgatata acctatgagg
    33421 cagcagttgc taagctaatg tttttgcttg gcaaagggtt ttatggtaag gcgcttgccc
    33481 atgagttaga aaaatccata gcaggcgaaa taacattgaa atagtcattg tttttttcaa
    33541 tatttttttt gtaatttgcg ccacgtttta aaggttcttt atttagtatt tcgtaacttt
    33601 ggagagatgg ccgagtggtc taaggcgcac gcctggagag cgtgtatacg ccaaaagcgt
    33661 atcgagggtt cgaatccctc tctctccgcc agccttttaa tgtttaacta aatttgagaa
    33721 caaacaaaac aaacctttag taataattaa acgacaatca aaccatgaaa aagctattca
    33781 cactagtagc agtattggga gtgctctcgt ttggagcatc aacctttgtt aatgcacagg
    33841 atgaacctgc agcaaaacct gactcagcaa tggttcagga gcaagcagct caggctgcaa
    33901 cacctgcagc tcctgctgtt gaagaacaat cgttacacaa ggctattaaa accaaattca
    33961 ttgagggtgg tcctggattt atggcttcca ttcttttaac cttaattttt ggtttagcta
    34021 ttgtaattga gcgtattatt tacctaaacc tttctactac caataccaaa aagcttctca
    34081 atgccattga ggatgctcta aaaaatggtg gtgttgaggc agcaaaggaa gtgtgccgca
    34141 atacccgtgg ccctgttgcc agcattttct accaaggtct tgaccgtgtt cacgagggta
    34201 tcgaagttgt tgagaaatca attgttacct acggatcggt tatgatgggt cagctggaaa
    34261 agggattaac ctggatttca cttttcatcg cagttgctcc tatgttaggg tttatgggta
    34321 ctgttatcgg tatgatcggt gcttttgatg ctatccaagc tgctggtgac atttctccgt
    34381 cgcttgttgc tggtggtatt aaggttgctc ttatcactac cgtgttcggt cttattgttg
    34441 ctattattct tcaggtgttc tataactacc tgctcaataa gattgatagc atcaccaatg
    34501 aaatggaaga cgcttcagta agtctgctcg atattcttgt taagtttaac cttcaaaagt
    34561 aattggccat gaatagctca ttatcaacct ataccaaggt tctttcgtgg ctattaatgg
    34621 gtgtttctat tgttataacc atactattct ttttgagcaa ccaggatatt gaatctgtag
    34681 aaccattcat tgattgggct tatattctgt ttgccatagc agccgtttcg gcattagttt
    34741 tccctgttgt aaaccttgta agaaacccca agaatgtcac taaatcgttg gtaggattac
    34801 tggcagttgc tgtaatcttt gtaattggct atgttttggc cgatgctact ccaattccat
    34861 cgccaactca aaacgctgac ctttcaaatc ctaccgttct gattattagc gataccggtt
    34921 taatagcaac atacctgtta tttggcattt ctatgctatt gttgttatat accggcgttc
    34981 gcagtgcatt ccataagtag tttaaacaaa tggttagccg gctcagttcg gctaacctga
    35041 aaaatactaa gtataaagat ggcaagaaaa gttccagaag taaatgccag ttcaactgcc
    35101 gacattgcgt tcttattgct catcttcttc ctgatgacca ctactatgaa cgtggatacg
    35161 ggtcttacga ggatgcttcc accaatgccc gatcccaatc aaaaacagga tgatgttgag
    35221 gttaagcgca ggaatatttt ctctgtactt atcaacaaga gcgataagtt acttgtccgt
    35281 ggtgaaccca tggaggttgg catgttgaag gagaagacca aggagttcat tatgaacccg
    35341 aatagcgacc ccaacctttc cgagtttgag gaaaaggata tacctggaat aggtaagttt
    35401 aaagtttcaa agggtgttgt ttctcttcag aacgaccgtg gtacatcgta cgaaatgtac
    35461 atgaaggttc agaacgagct ggttgctgca tacaatgagt tacgcgacga actatcaact
    35521 tcagtttacg gaaagccttt cagcaagtta gccaaggaag agcaagaaac aatttctaag
    35581 gccatcccat tgaagatttc cgaagctgaa cctaagaacg taggaggaaa gtagttatgg
    35641 ctaagtttga acgaaaaggt aaaaacgagt tgcaggctat ttcaactgcc tcgcttcccg
    35701 atattgtgtt catgcttctc ttcttcttca tggttagtac aaccatgcgt gaaaccacgc
    35761 taatggttcg tagccagctg cccgaagcca ctgaagtaaa caagctggaa aagaagtcgt
    35821 tggtatcgta tatctacatt ggtcctccta tgaggcagtt ccaatctctg tttggtactg
    35881 agccccgcat tcagcttaac gattctttca agtcgctcga cgatattcgc gattttatcg
    35941 catcggagcg tgaagcaatg aaggaagatg aaaagccctt gcttaccaca tcgctaaaaa
    36001 ttgacaagga aacccgtatg ggtattgtta ccgatgttaa gcaggagttg cgtaaagcca
    36061 gtgcactcaa gattgtttac gctgcccgca aggccgataa gaaaaagtaa tttgcttttt
    36121 ataccattat ttcaggaagc tgcccttggg cagcttcctg ttttttaaca ccttttaata
    36181 aactgaacta aaggcttgta tgttgctcca acttttttag tgtagttttg ttatcaatac
    36241 attcaataaa ttttttaaat cacaagagat gagagcacga accctaataa caattttgct
    36301 cgctatagca atggcttcct gcgggaaaca tgaacccaag gcaaagtatg tattttactt
    36361 tattggcgat ggaatgggag tatcgcacgt gacacttgca aagctgtatg ccgatagcgt
    36421 tttactcgac agcacggcaa tgtcgtttac ccgtttcccc gtcacttcgc tttctactac
    36481 ctatgcacat aacaggtatg ttacctgttc agcagctgcc ggaactgctc tttcaaccgg
    36541 taccaaaact tcaatcaaca ccattggttt aacttacgat aggacagata ctttatacag
    36601 tattgcaaaa aagtttaagg aagctgggaa aaaagttgcc attttaacca gcgtaagcat
    36661 tgaccatgca acaccggcag ctttttatgc ccatgttggc tcacgtggca gttactacga
    36721 tattgctaaa cagctgctga agtcaaatta cgattttttt gcatcgggtg gtttccttga
    36781 accctatggt accaaaaccg attccaatgc cgttagcgta tacgagttgg gaaaggttag
    36841 aggttgccaa tttacatcat cgctaacgga ggtagactct ttggtggcag caggagcaaa
    36901 atccattgtt tactctgccc caaatcctgc acccagcagc acattacagt atgaaattga
    36961 ccgttcggaa tccgatataa gccttgccga cattacccgt aaagccattg aggtagtgga
    37021 taaccccaat ggtttcttta ttatggttga agggggcaaa attgattggg ctgcccatga
    37081 caacgatgca gcaactgtga tacatgaagt gcttgctttt gcccgtgcta tagatgttgc
    37141 cttggaattt tacaggaaac atcctcacga aacgttaata gtggtaactg ctgatcacga
    37201 aactggtgga ctttcaattg ggaacaggga aaacaagtac gattttcacg taaagttact
    37261 ccattaccaa actgcatccg aggagcgttt acataagctt attgctgaat ttaaggaaca
    37321 aaacccaaag gcaaagtttg acgatttgct gaagttcttg gccgataaaa cagggctttc
    37381 aaaagatatt gagctaaacg aaaacgatat caagatgctg aaagatgcat acaacgtagc
    37441 ctttaagggt ttaaaatcaa atgaaaaaac cacatacagc gaaactgatc ccatatcaaa
    37501 ggtttccatt cagatactaa actcaaaggc tgcagtaggg tggacatcgg gctcacatac
    37561 tggtcaaccc gtgccagtat ttgcaattgg cagtggtcag cagctatttg ttcctttgct
    37621 cgataacacc gatatcccca aaaatattgt tagagctgca ggcatatagc ctaaaccata
    37681 agcgaaatta aatataaggg aaggttaaac cttcccttat ttatttgacg aatgcaatga
    37741 attgttgctt taattaaatg caatctgctt taaacaaacg ttttttggtg atatttatca
    37801 atccatatgg ccaaaaaaaa tttttaaata aaaaaattgc cgtaacttta ttcaataact
    37861 ttaaagtgat aaagtatgaa aaagggatat ttgcttttgg ccttactact tagtgccaat
    37921 atttacgctc aggatattga aaaagggaat cgcattccat ccacctacga ccgtagttcc
    37981 atcacagtgt tctacatgaa gttcccgggt gaaaaccatg cctcggaggt ggaagcaaaa
    38041 tttccggaag taaagtttac cgataagtat tacaacaaca atctggatgc tttggtattt
    38101 gaagcaccct ttactcgagg cgatgcctcc gttgttccaa ccaaagccat cgaaagtttt
    38161 ctgatgcaga aaggggtgcc aaagtcaatt attagtaagt ggtacaaccg taaggatgat
    38221 ggctcaatgg atctggacct cattttcgat aggggtatgt ttaacgctac cgacgcccag
    38281 tacataaaag cccaagccac aaagcgaggc gataacatac ttaaggatta tgggaaccgc
    38341 ctgattgaga agagttacat cctggtgctc gattaccaga gtatcctcac catgaaggag
    38401 gcaaaggttg agaaaatgaa gggatggaag gcaaccgtta atggatatct ttataaagta
    38461 aagttcaatg aggaaaccca gaatgcgctt tacgattgct ggatatatcc tgaagattct
    38521 cccgctgttc gtcaggaaaa agttaaaaag ttccaggaac taaatattcc tattgagttt
    38581 gttactcaaa ctgttgtata tgccaccgcc tcgcaaccca cagaggatac tcagcttggt
    38641 aagctaatta aacaaaaaac cgaggatcag ttacttacag aacttgttca aaaaggatac
    38701 gatgaaagcc tttactacct tgaaatgaaa catgaagatt tcatggtaaa aactaccatt
    38761 catcaggtta ggccaattcg ggctaaaatt gggttgaagg aaggattaaa atgtgatcat
    38821 cgctactttg cctatgagta tgtttacaat gaaaaaacca acagcgtgga gccacgtttt
    38881 agaggtgtaa ttcgcgcaac cagtaagatt gtcgataacc ggcaggttgc taaaggcgat
    38941 atgcctacct cgcagtttta ccaaactgct ggtagaaggc tacgcgaggg atatctgatt
    39001 cgtcagcaga acgatcttgg cctagagtta ctggttggct atgaagctgg tgaggttggt
    39061 ggtatttacg gcagaattga tgcacgtatg ggtcgatatg ttggtataaa agccctgttt
    39121 gtttacgttg agggtggtgt tcaggcaaag gattatgaaa ttggatttgt aaattacgag
    39181 aacattgcct tcttacacta cggagcaggt ttggctaagg gattcatgct aactcgcaac
    39241 ctggaattgc gcccatatat tggtttaggg caggaacagg ctaaccaatc ggatttgtcc
    39301 agtagcacgt taaaagctct ttacctgaaa gctggggcaa atttggcact aaatttgaaa
    39361 cataacatcc agctaatggg tggtattggt tcatactcat ttatcagtaa tgcagaagaa
    39421 ggaggtactg ataccggatt aacatgggac gaaaaattta ctgaccgtaa aggtgcagca
    39481 gtaatgtttg gtattaaatt tatgttctaa ctttaaaata gtataggtgt atgagaaaag
    39541 ttttattagg atttacggtt gcagtgcttg ctatggtaat ggttttaccg actaatgcac
    39601 aaacccgcaa ggagcggaag attttatgga atagccagta taactatgaa attgaaccgg
    39661 ttggtgttgg acaggatggt acaaaagtgt ttaaggtttg gggatacggt aaggatgttg
    39721 gcactgcagt tatgaatgcc aaagaggctg ctgtagcagc ttgtatattt aaaggactac
    39781 ctggcggagg aggctctgct cctacccctc ctatttgctc ccagcccaac gctgaacaaa
    39841 ttcatgccga ttactttgaa aagttctttg aggtgggagg taagtacctt cagtacattg
    39901 ccgtaaccaa cgatgcagac ccttcgggtg aggatcgtat taaaatgaag aaaggataca
    39961 aggtggcaat ctacgttcag gttatgtacg atgctttacg taaacagctc gaagccgatg
    40021 gcatcgcacg tcgtctggat tcaggattct aatacatgat gcaaatatga aacgaatact
    40081 actatcaatt tcgttgcttg ccctcgcggt tatagcattt ggtcaggcaa aaaagcccat
    40141 aataatggtt gttcccagcg accagtactg cattagccgc gggtacaaaa tggagtttaa
    40201 caaccagggt ttgaaacagg ttctccccga ttacaaaacc gccctgcaaa ccgatcccga
    40261 tttacgtttg gttataacca aaatgggcca aataatggcc gataggggtt tccctcttaa
    40321 agatctggag caggagctta aaaacctgga gcaggaacgt gccgaatcgt caatgctgac
    40381 aagctcatca agtggttcgg agatggccga aagcccaatc gatgctttaa agcgtgttgc
    40441 caaggccgac atcataatgg acttggactt tgaagtaaag cgtcagggac ctcagaagta
    40501 cattgtgttt aacctaaaag ggctcgatgc atacactgca aagcaggtat cgggtgttgc
    40561 aggagcgggc agcccatcta catcagcttc gccagagctt ttgctcgagg aagcagtgct
    40621 tagccatatg gataacttta acgccggcct aatgcgccat tttgaggata tgtttgcaaa
    40681 tggccgtgag gttaaggttc agataaaggt ttgggctaac tggggcgaga atctggaatc
    40741 agagtttggc agcgatggca
//
LOCUS       DAOOWJ010000008        38599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1096_length_38599_cov_9.222914, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000008 DAOOWJ010000000
VERSION     DAOOWJ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 38599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38599
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_38599_cov_9.222914"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..935
                     /locus_tag="PLW22_01685"
     CDS             138..935
                     /locus_tag="PLW22_01685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOU73443.1"
                     /translation="MEKGLIFDIRHFSVHDGPGIRTTVFLKGCPLRCVWCHNPESQKY
                     EPEKVQRTTKLDGKEYNSSETIGSYMTAEEVFDQVKTHIPIFDESGGGVTFSGGEPLM
                     QPKFLVNILKLCRANDIHTAVDTCGFALHEVFNLILPYTNLFLFDLKLISPQKHESYT
                     GQTNHVIFKNLFLLELNNAPTIIRIPLIPGITDDQNNLEAIKSVLKPLRCVKRVDLLP
                     YHHMAKDKYRRMNLPYKLEDLKSYTANQLGEIKSFFNDIDIPVTIGG"
     gene            956..3313
                     /locus_tag="PLW22_01690"
     CDS             956..3313
                     /locus_tag="PLW22_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOU73444.1"
                     /translation="MMTERIQQLRKQSLNAVNTLSAERALLVTEYYKKADSNLPIPIL
                     RARAFEHIMLNKKICINPGELIVGERGPAPKAAPTYPEVCLHSLDDLEIINSRPKVSF
                     RVDEETREVYRNIIIPYWTGKTLREKIFAWLSNEWHQAYSAGVFTEFQEQRAPGHTVS
                     GRKVFQKGMLDLINDIDSSLKCIKQEANTREKVNELEAMRIVANAIIGFANRYADNLH
                     LLAEKETDENRKAELQKMEVICRRVPTHAPQNFHEALQHYWFIHVGVITELNPWDSFN
                     PGRLDQHLYPFYRRDIENGTITQEQAIELLQAFWVKFNNHPSPPKMGVTAQESNTYTD
                     FALINLGGVKEDGSDAVNELTYIILDVIEEMRLLQPSSMVQVSKKNPDRFVKRAVKIT
                     KTGFGQPSFFSTDAIVQQLLSQGKSLVDARNGGASGCVETGAFGTEAYFLTGYFNLNK
                     VLEITLNNGVDPLTGKKIGLETGNPENFKSFEEFYEAFERQLNYFIDIKIEGNLVVEK
                     LWAMNMPVPFLSLLIDDCIANATDYNAGGARYNTSYIQGVGLGSITDNLTAIRKHVYE
                     DGSIGMKEMLFALSSNFDGYDEFRYKLIYETPKWGNNDEYADRHAVMVFNSFYKAVNG
                     RPTYRGGVYRINMLPTTSHVYFGSKIGAMPDGRKAFEPLSEGISPVQGADRKGPTGVI
                     LSCSKIDHIKTGGTLLNQKFSPSFFSTDESLDKLVMLIRSYFKLDGHHIQFNVVSANT
                     LRDAQKHPEKYRDLIVRVAGYSDYFNDLGEALQNEIIRRTEHESL"
     gene            3348..4646
                     /locus_tag="PLW22_01695"
     CDS             3348..4646
                     /locus_tag="PLW22_01695"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HOU73445.1"
                     /translation="MKRIFSPLLLLIVICLFLLGFQRQNPVAEQVLKSIPIVDSFKLI
                     DNDPLFQTTYEVWFNVPLDHNNPNSEKFPLRAYYSHRSFTRPMVVVIDGYTMYTSRPN
                     ELTRILEANQLTIEHRFFSKSRPKDSIPWSYLNIRQAAADAHAVISAFKPHYQGKWIS
                     TGISKSGQATIFHRRFYPNDVDVSVPYVAPLNFSSEDKRVYEFLSKVGSAECRKKIRE
                     FQIALFRNKNTLLPMFEGLAQKNNWQFTMGIGRAYDLSVLEYEFAFWQWGYSCSDIPA
                     GNSQPSVLFEHWSQVNPFTFFEEKEIEKVRPFFYQAMTQIGMYGYNIEPFRNYLPDKK
                     NITFEFTLPKGHTVSFDSSAMLDVYRWVRDSGNYMLYIYGQNDAWSSTAVDPGTKTNA
                     VKMVCPSGSHSTRIKDFPKPLRDSIYITLERWLEMPVGRK"
     gene            complement(4691..5392)
                     /locus_tag="PLW22_01700"
     CDS             complement(4691..5392)
                     /locus_tag="PLW22_01700"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOU73446.1"
                     /translation="MNRIIITSLATAISVLSMAQNTLLTQRVSELNFYQLPYQYAALE
                     PAIDALTVEIHYSRHHKAYFDNFLKQASDLKLTEKSLNEIFAKVSEYPAGIRNNGGGY
                     YNHVLYWEILKPGNRKGLSPTLNKAIADEFGSVENLIAQMNDAALKRFGCGWAWLVVD
                     GNGKLKIGSTPNQDNPLMDVSDFKGIPLIGIDVWEHAYYLKYQNRRAEYVNNFWSLIN
                     WEIVSELYNEAIKKK"
     gene            complement(5925..7505)
                     /gene="nadB"
                     /locus_tag="PLW22_01705"
     CDS             complement(5925..7505)
                     /gene="nadB"
                     /locus_tag="PLW22_01705"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOU73447.1"
                     /translation="MSKIETDFLIIGSGIGGMSFALKVADHAKVIVVTKDRLDETNTA
                     YAQGGIAAVTYDPDTPEKHIHDTLICGDGLCNEEVVRIVVNEAPSQIKQLISWGVKFD
                     RKPNGQYDLAREGGHSEHRILHHKDNTGAEIQKVLSNKVRNHPNITVYENYFAVEIIT
                     QHHLGQEVKRGNPDITCFGAYVLNLKTQEVHTFLSRVTVLATGGTGNIYNTTTNPPVA
                     TGDGIAMVYRAKGQVENMAFVQFHPTSLYNPGERPSFLITEAMRGFGGVLKTIDGKEF
                     MQKYDPRGSLAPRDIVARAIDNEMKVRGDDYVYLDVTFKEPKEIIDHFPNIYQKCLSL
                     GIDITKDMIPVVPAAHYMCGGIKVDTNAQSTIKRLYAVGECSSTGLHGANRLASNSLI
                     EAIVYAHRGAQHSMEHYKSYEINHSIPEWDYEGTIHNEEMVLITQSYREMQQIMSSYV
                     GIVRSNLRLQRALTRLEILYRETEELYKKSILTQKLCELRNMINVGYLIIKSAQEMHE
                     SRGLHYSIDYPKQKGQEF"
     gene            complement(7582..8355)
                     /locus_tag="PLW22_01710"
     CDS             complement(7582..8355)
                     /locus_tag="PLW22_01710"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOU73448.1"
                     /translation="MYPDYHMHTNFSDGHHTHDEMVYAAEASGIYEIGFTDHISLKPI
                     GWAMDLGRVPELIDTVNRIQHKSNSVTIRFGAEVDYIPNLVNETRGVLQSIPLDYTIG
                     SVHFIDDWNFDTDINGYQGIDIDDFYRTYFKLVGEAAQTGMFDIIGHADLAKKFAVYP
                     TFKLQKIYERCAQIFSDCGVVVELNTSGKNKPCAEFYPSVDFLEILHHYRVPITLGSD
                     AHVEQNIGQYFDEAVNLLKSLGYKEIITFSQRKRIPINI"
     gene            8452..8724
                     /locus_tag="PLW22_01715"
     CDS             8452..8724
                     /locus_tag="PLW22_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HOU73449.1"
                     /translation="MITVVSIMAGGMLLGFLLRAKQRIVSGNEKLITYAIYLLLFMMG
                     VSIGSNEQIMNSLSTLGIVALIVSMGAIIGSILTGFLVFKLFFKND"
     gene            8727..9329
                     /locus_tag="PLW22_01720"
     CDS             8727..9329
                     /locus_tag="PLW22_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOU73450.1"
                     /translation="MKGSLTILSFFTLGLIVGRYTPVNDWLSSVDYSSLSLYLLMFLV
                     GISIGSDTRSLQALKSMNLKILLIPLATIIGTAIGVWFISIFVSRYSIKELMAVGAGY
                     GYYSLSSIIIRNSHSDALGVVALLSNVIREIITLLLAPLMAIYFGKVAPICSGGATSM
                     DTTLPIISRASGKEYAIPSLIHGIILTILVPFIVTLILSI"
     gene            9341..10132
                     /locus_tag="PLW22_01725"
     CDS             9341..10132
                     /locus_tag="PLW22_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOU73451.1"
                     /translation="MKIKTLLLAAFAFLLLISCKTKTEKENFNQDHLIYATIWYQNSP
                     EMKALYYQGFNIAEERLKQYTKLKTAKPKAVVVDIDETMLNNVPFQAQEIIDNKEFSS
                     EFWNEWSQLARAEATPGAIEFSKLCDSLGIPIFYISNRKVNEFDATLRNLDSLGFAFA
                     KPEFIILRDNESSKKARREKVMENYEIVLLIGDNLNDFSEVFEDRSDSWGTPTVEKFK
                     NEFGSRFIVLPNPMYGDWEKNIYPSRGMLPAQQDSARRSVLKGFK"
     gene            10300..11400
                     /locus_tag="PLW22_01730"
     CDS             10300..11400
                     /locus_tag="PLW22_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU73452.1"
                     /translation="MRSLAHLLIPALFLFSCSKSNHEPTVYFTKDITPEGFMKVYKQI
                     SKNLEGKVGVKVHFGEEGNSYYVKPELFKDIVLDCKGTFIETNVLYKSPRQKTETHIA
                     LAKQHGFTYAPIDILDDKGELVIENVPGCKHFNKFYFGKNIDEYNSFLIISHFKGHGS
                     AGFGGAIKNISMGFATPRGKLAMHRNNYPVCDRDRCTNCDSCAQQCPANAISTNPIWI
                     DVDKCTGCGKCIDVCPNKALRYPKGDVDVQTVFCERLAEYAKAIYDRHKKMVFINVLV
                     DISTSCDCSRNPGKPFVPNIGILASTDIVAIEKASHDLVAKAHGCADPFLEVNRVSGY
                     RQIQYAKTLGMGKMNYRLVNLDNNQETVVISK"
     gene            11471..11542
                     /locus_tag="PLW22_01735"
     tRNA            11471..11542
                     /locus_tag="PLW22_01735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11504..11506,aa:Arg,seq:cct)
     gene            11684..12169
                     /locus_tag="PLW22_01740"
     CDS             11684..12169
                     /locus_tag="PLW22_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOU73453.1"
                     /translation="MAETYKVDAIDQKILSYLVKNARMPFLEIARECGISGAAIHQRV
                     KKMEDAGIIAGSRMVVKPKALGFDVCAFVGVQLVSTNLYAGVIEALKMMPEVVECHFI
                     TGEYALLLKIFCRDNEHLMDVLVNTIQNIPGISRTETFISLDQAIERPVYVKDKSVIK
                     G"
     gene            complement(12234..12458)
                     /locus_tag="PLW22_01745"
     CDS             complement(12234..12458)
                     /locus_tag="PLW22_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73454.1"
                     /translation="MKSKKDFRKPVKNDSFDSDELKRAKKLKPVKKEKNLKRSFYEEI
                     DEFEDLDDYDLKDSLDDYYDDDAYDEEEDY"
     gene            12715..13665
                     /locus_tag="PLW22_01750"
     CDS             12715..13665
                     /locus_tag="PLW22_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU73455.1"
                     /translation="MGSEVIIHQLLIFGVLMVVGALGSYFGIIKDEAKDFLSRFIIDI
                     TLPCLIFSTFAKIDSNPTLLINGLLVFGFTFVNLAIALFAGTVSARLQGLNPANTTVH
                     SLHTMFGNIVFLGYPLFDALFPGGIGIFYAAAYQLASNSVTFTYGIYRLSAGQAKSGL
                     KSLLNINTFALLIGFTITAFGIKIPAFFIDALSGLGKATSPLSMVYIGALLMGLGIRK
                     SVSHKSIYLLSLNKLILLPVFLGFIYLWIFRFFNLQISSEAFIVLVMQMAMPCQTIIV
                     VMSRRYGGNYQLAAGNLFVSTILSIATLPAIYIYLNFLLK"
     gene            complement(13669..14229)
                     /locus_tag="PLW22_01755"
     CDS             complement(13669..14229)
                     /locus_tag="PLW22_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOU73456.1"
                     /translation="MKFLTLLTALLISTTVWSQEFIADASSSTLKWNAKKVTGEHYGK
                     VKLKEGKLVIRDNQIAGGNFVIDMESITCDDLESPEWNKKLIDHLKSDDFFSVGTYRV
                     ALLGNVRSDKFNGDKAKVTTDLTIKGITKPITFEVIKKGNVYTSTITVDRTQYNIRYG
                     SGKFFEGLGDKMIYDEFTIEVKLVVK"
     gene            14320..14775
                     /gene="dtd"
                     /locus_tag="PLW22_01760"
     CDS             14320..14775
                     /gene="dtd"
                     /locus_tag="PLW22_01760"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOU73457.1"
                     /translation="MRVVIQRVSQASVSVNGSITGSIGNGLLVLAGFEHSDTEEDLEW
                     MVGKIVRLRIFNDAEGIMNLSVMDVNGQILVVSQFTLHAKTKKGNRPSYVQAAPPDIA
                     IPLYEKFIMMLEHATSKRVERGVFGAMMDVSLVNSGPVTIIIDSKNKDL"
     gene            14772..15107
                     /locus_tag="PLW22_01765"
     CDS             14772..15107
                     /locus_tag="PLW22_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOU73458.1"
                     /translation="MTQLTLDDAQKLVDNWIKTKGVRYFNELTNMTLLVEEVGELARI
                     MARRYGEQSFKEGETDHDPAPEMADILFVLICLANQTGINLTQALLQSIEKKTKRDSE
                     RHQGNTKLL"
     gene            15145..16044
                     /gene="deoC"
                     /locus_tag="PLW22_01770"
     CDS             15145..16044
                     /gene="deoC"
                     /locus_tag="PLW22_01770"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOU73459.1"
                     /translation="MDLSFEDKFNIEVEAAEVEKAINKFKDIAQSRKGDVEILKRCFS
                     MIDLTTLNTTDGYERGKLFAEKVSQFPNQFPGMPNVAAICVYPSLVAAVKENLSAPSV
                     KIASVAAGFPASQTFLEIKLSECEMALEQGADELDVVISVGSFLEGDYFTVFSELQQI
                     KEVAENAKVKVILETGALPSLEDIKLASFLAMEAGSDFIKTSTGKLEPAATPEAVYVM
                     CLAIKEFHEKTGRKVGMKPAGGVSNSYDAALFYTIVEQVLGQEWLYPGLFRFGTSRLA
                     DVLLSDILGKDVKYFSTSSSAKY"
     gene            complement(16117..17715)
                     /locus_tag="PLW22_01775"
     CDS             complement(16117..17715)
                     /locus_tag="PLW22_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HOU73460.1"
                     /translation="MSFATVDYIVFIAYALLIVSLGLWVSFRKRDSETNSGDYFLAGR
                     ALPWWAIGASLIASNISAEQFIGMSGSGYAIGLGIASYEWMAALTLILVAVFFLPIFL
                     EKRIFTMPQFLEVRYDKRVKTVMAGFWLLVFVFVNLTSIIYLGALAMQRIMGINMTYA
                     IIGLAFFAALYSIYGGLKAVAWTDVVQVVFLVGGGLVTTYFALDYLSDGHGFIAGFKA
                     LLQKAPEKFDMILEKGKLMIPDGKGGQKDAYIDLPGISVLIGGMWVANLYYWGCNQYI
                     IQRALAAKSIKEAQSGLVFAAFLKVLLPLIVVIPGIVAFALHADIAKSDEAYPWLLHN
                     FVPTGVKGLAFAALVAAIVSSLASMMNSVSTIFTMDFYKEYINKDASEHKLVTVGRIA
                     AFVALLIAVFIAPMLSNLDQAFQYIQEFTGFVSPGALAIFLAGFFYKKATANGALGAA
                     VGTFVFSLLFKLLLPNLPFMDRMGIVFLICLAIIWIFAMVEGNKQHVKAITISKELFS
                     TGKGFKIGSAIIIALLIILYAIWW"
     gene            complement(17815..18723)
                     /locus_tag="PLW22_01780"
     CDS             complement(17815..18723)
                     /locus_tag="PLW22_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU73461.1"
                     /translation="MKPTLVILAAGMGSRYGGLKQVDALGPNGETIIDYSVYDAIRAG
                     FGKVVFVIRKSIEKDFLEVFGNRFDSDVRVEIAFQELDMLPQGFKVPDGREKPWGTAH
                     AVWVTRHLINEPFAVINADDFYGFDTFRVLAQELSKPNLEKGSYFMVGYKLGNTLSEQ
                     GAVSRGVCSTDKNGYLLDVVERTHIERVEGEVKYKDENGNFVKVDEGVPVSMNFWGFT
                     TDFFGYTEKLMTDFFSNQIGNVKAEFYIPTVVNRAIKEGIARCKVLETKAEWFGVTYP
                     GDRPMVVNRLKELTEKGVYPSPLWSR"
     gene            18897..19769
                     /locus_tag="PLW22_01785"
     CDS             18897..19769
                     /locus_tag="PLW22_01785"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU73462.1"
                     /translation="MKRFIFLVALLNVLIISTNAQNKTKSQLEADFEKFKKEREQDFQ
                     DFKQKREAELKKMEQEYLDYYNEMTGLKKHYVEKKEPEKAQTVQDIIDYENTIAKTLG
                     YKIPERVKEKVEANTKSEVSKKEEAKTEAENVNVEDKKPEVSSSTFKPEAGTRDGIPV
                     LTPLPKRLAKITSPFGVRIHPTLGRPIKHNGVDFGSGRGAEVYAASSGKVVLAQYNST
                     YGNYIIIEHNDGTSSVYAHLDKIKVSKGSPIRKGDVIGYTGSTGRSTGPHLHYEVRIK
                     GIPVDPKGYLVETK"
     gene            19866..21116
                     /locus_tag="PLW22_01790"
     CDS             19866..21116
                     /locus_tag="PLW22_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOU73463.1"
                     /translation="MLKREDIEVMAPAGNFESLMAAIQGGANSVYFGVGNLNMRAKSS
                     INFTVDDLPEVVRICKKNKVKTYLTVNTVIYDSDIPYMHQIIDTAAEAGVSALIISDQ
                     AAMLYARKRGMEVHISTQLNISNTDALKFYSQWADVVVLARELNLEQVKTIYQNIHSQ
                     NITGPNGNPIKIELFAHGALCMAISGKCYLSLHEANSSANRGACLQICRRSYTVTDNE
                     TGAQLEIDNEYIMSPKDLCTINFLDKILDAGVRVLKIEGRARGPEYVKTVTQCYNEAV
                     TSVCNGTYTPEKVENWMQRLSTVFNRGFWDGYYLGRRLGEWSKAYGNIATERKQYVGK
                     ITNYFSRLGVAEITVEASELYLNDKILIIGPTTGVIEYEVKELQVDEKPAEFSPKGVK
                     CSIPVPDKVRRADKLYRIVKMNSD"
     gene            21161..21778
                     /locus_tag="PLW22_01795"
     CDS             21161..21778
                     /locus_tag="PLW22_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HOU73464.1"
                     /translation="MSFSQLCNKIFWDCTLHYHTMDNVDAQPNIPYQPGTIEFSLFTK
                     NWIDAVQWHLEDIIRDPKIDPVEALKIKRRIDKSNQDRTDLVEMIDSYFLEKYKDIKP
                     QSNATINTESPAWAIDRLSILALKIYHMEQEVKRTDVSKEHIDSCQKKLNTLLEQRVD
                     LSTAIDQLLDDIEHGRKYMKVYKQMKMYNDPSLNPVLYGAKQQKG"
     gene            21753..22829
                     /locus_tag="PLW22_01800"
     CDS             21753..22829
                     /locus_tag="PLW22_01800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOU73465.1"
                     /translation="MEQNSKKVNGYPKKLLVVRLSAIGDVAMTVPAIWSLVKNHPDVQ
                     VTFVSQGFARDIVNQIPGVLFVEANTKGRHKGLLGIWRLFTDLRKIDRYDALADLHNV
                     IRSQILRNLFLLSGLKKISVINKGRSEKRKLVRLKNKRLAPLKTTPERYMDALLRLGY
                     TINDEFDYLFREARLPENITIHLGYKDKRWIGIAPFAKHKGKIYPLDKMERVVEILSA
                     SKTNKIILIGGGGKEQEILEGWEKKYPNTISIARKYSILNELKIISNLDVMVCMDSAN
                     MHFASLVNTPVVSIWGATHPYAGFYGWKQNPTSAVQVDLYCRPCSIYGNKPCFRKDYA
                     CLNLIEPEVVVSKINTVIDRFNRI"
     gene            22843..23754
                     /locus_tag="PLW22_01805"
     CDS             22843..23754
                     /locus_tag="PLW22_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOU73466.1"
                     /translation="MTYVTKEKLFSREWFKAYALILVGSFILAAGFVLFIDPHRIAPG
                     GVYGIGIIVHYLTKGMFSWAPDGFPIGTVGLLLNIPLTIIGIRVLGPMFGIKTVVGFV
                     LSSVFIDTLHLWLGYDPLVDDMLLSSIFGGVLIGFGLGLIFKSKATSGGSDIIAMIIA
                     KYTRLPLGQLMIYVDSTIVLLALVAFQDWKIPLYSWVVIYVTGKVIDMTIQGVSYDKA
                     LFIITDRHEEVKNKIIHDIERGGTIINVQGMYTGHDKKMIFTNVSRREVHMLKDYIQQ
                     IDPNAFITVIDANEILGNGFKPLNESM"
     gene            23765..24700
                     /gene="rsgA"
                     /locus_tag="PLW22_01810"
     CDS             23765..24700
                     /gene="rsgA"
                     /locus_tag="PLW22_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOU73467.1"
                     /translation="MEKGLVLKSTGSHYRVRLDDGRVVECVPRGKLRLNEIKTTNPIT
                     VGDWVSVELNPNGVGLITAIQERKNYIIRKATNLSREAHIIAANIDQALLVVTITQPE
                     TQLAFIDRYLVTAEAYRIPTVIVFNKIDLVDSAIQPVLDSYISIYEPIGYKCLKVSAK
                     TGQNLDLLKQILQGRVSLLSGNSGVGKSTLINLIEPGLNLKTAEISNAHLKGRHTTTF
                     SEIFELSFGGFIIDTPGIKSFGLVDIEKDELYHFFPEIFKLSDKCKYYNCTHIHEPGC
                     AVIEAVDKSEIAPSRYLSYLSIYDDENEKYRPKLK"
     gene            24744..25511
                     /locus_tag="PLW22_01815"
     CDS             24744..25511
                     /locus_tag="PLW22_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOU73468.1"
                     /translation="MKSHEIDYRIIGESIQLVEIELDPHETVIAEAGAMVYMESGIDF
                     ETKLGDGSNPSSGFLGKLLSAGARAITGESIFMTHFTNHGIGKRKVAFSAPYPGTVVP
                     IDLSKVGGNLIVQKDGFLCAALGTSISLHFNRKLGAGLVGGEGFILQRLSGDGLAFVH
                     AGGTVIEKQLNNETLRIDTGCIVAFEPTIDFDVQTSGNLKSMLFGGEGLFLATLSGSG
                     KVWLQSMPIKKLVQALAPYGRNRGKENSSILGNLLED"
     gene            25514..26554
                     /gene="mnmH"
                     /locus_tag="PLW22_01820"
     CDS             25514..26554
                     /gene="mnmH"
                     /locus_tag="PLW22_01820"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOU73469.1"
                     /translation="MASTISIDKFIELSDIYPVVDVRSPSEYARGHIPGAFNIPLFSD
                     EERAAVGTKYVREGRQQAVRLGLELVAPKLNWFVDSLSSMVNQKNILVYCWRGGMRSA
                     SMSWLFGLAGFKPFTLEGGYKSFRRYAQAAFDKKYKFIVLGGMTGCGKTELLKKLKEN
                     GEQVVDLEGLANHKGSAFGWINQPQQPTTEHFENLLFNELRKLNPALPIWIEDESKSI
                     GSIFIPLSIYNQIISSPTIVVETDLKNRIDRLINDYTACDKQDLINSVVRIQKRLGLE
                     KAKRCIEYIESGNLRDAIAITLEYYDKTYRYGIEQKKIPPKFLTFDGTSNDMIVKLTS
                     VGKSITHPAFPD"
     gene            complement(26529..27368)
                     /locus_tag="PLW22_01825"
     CDS             complement(26529..27368)
                     /locus_tag="PLW22_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU73470.1"
                     /translation="MAENINKYLIPWDFTPVAENALKHAIKLMKLAKEPIEINLVHVI
                     QTGGLFNKSKFSEDDASQKLKDDQQRIVNEYGVEVKTHLLEGNIFDTISEFASEINAE
                     MVIMGTHGIKGVQKLTGSWALKVIAGSEVPFLVVQDEPDMAKGFSHIVMPVEFRDEDK
                     EKIQWGIRFAKLFGSKIHVIVPNAFDSGVQKKINNNLSFAKMHIESNGLDWEIHNAAK
                     GKSFQEEIVNLATDIEAELIIVMTTPNIDLTDFVFGAQEQYVIANKAKIPVLCINPGT
                     LGV"
     gene            complement(27449..28423)
                     /locus_tag="PLW22_01830"
     CDS             complement(27449..28423)
                     /locus_tag="PLW22_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU73471.1"
                     /translation="MYTLESINQKVDSAIKQLDLTGEPRNLYEPIAYIMSMGGKRIRP
                     ALVLAACNLYSDDIDQALPAALAVELFHNFTLVHDDIMDNADMRRGLVTIHKKWNPNI
                     AILSGDAMTVLAYKLLVQVDKSILPEVINIFNSFAMGICEGQQMDMDFESDTMVSRDE
                     YLKMIELKTSILLKGALQIGAVIGGAGRNDVELIGEFGRSMGLAFQLQDDLLDCYGDS
                     STFGKRIGGDIVASKKTMLTIITAEKLKGTQRSDFIELYNSKSLTDQLKIGRVMDYYS
                     KAEVQHEMVSIIDTYFDNATQAINKLNVSESRKDVLNQILTRLIGRKN"
     gene            complement(28436..30787)
                     /locus_tag="PLW22_01835"
     CDS             complement(28436..30787)
                     /locus_tag="PLW22_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOU73472.1"
                     /translation="MFHKLARFILKRRFILVSILLVFTFFMGWKATKVRLSYKPIPLV
                     PKTDSIYIQNERLAKLFGKGENIMVIGVTDSAFFSSDRVLLWENLSNKIKQVDGVENV
                     FSVIDIITLEKDTITRKFVAKKLLDNLNTISPDSLSNQVKNLPFYKGLLYNAETNTYL
                     MLITLNREKMTRVDRIKIINQVKEASQEYEARTGNTLHYSGLPYIRAVVAEMIKYEMF
                     LFVGLAALVTAIILLVLFRSFRVLFISLVVVGTAVIWSLGSLTLFGYDLTLLTAVVPP
                     LLIVIGVPNCIYLINKYHHEIAHHRHKIKALYRVIYRIGSATFLTNLTTALGFVTFVF
                     TKTQILQEFGLIAFLNVLGIFLISLIVIPFFFSISPTPKARHLSHLNRNTFKQAVSRI
                     VLTTYYKRKWVFAITVFALVIGIFGASLLKANGYMVDDVPQNHPVQRDLKFFEKNFTG
                     IMPLEIVYNTGKPNGYQNYTVYQKVERLQEKLSSYPELSKPFSIAEIAKFARQAYYNG
                     NESQYRLPGPFERAFIMSYVPRSIGVSDVYARMTDSTGQYVRIMYNVADIGSEKMSIL
                     FDSINSDIRQVFDDEHTNVHIAGASLITTYGIKYLVHSLSSSLIFAIILIALAIAWLF
                     RKFRMVLFAIGINLIPLILTAATMGFLGINLKPSTVIVFSIALGIAVDNSIHFLTKYR
                     QELYLTKFNIKESIFHAIRETGISMIYTSIVLLFGFSVFIASRFGGTKALGLLVAITL
                     LYAVITNLFVLPSVLRQFDKPKPLKPEEEELVMEMSSGFDDEE"
     gene            30964..32694
                     /locus_tag="PLW22_01840"
     CDS             30964..32694
                     /locus_tag="PLW22_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOU73473.1"
                     /translation="MNYGLLDLLNLIGSLGLFLYGMKMMSESLQKVAGDKMRAILSSM
                     TSNRFMGILTGFLVTAVIQSSSATTVMVVSFVNAGLLSLVQSIGVIMGANIGTTITAW
                     LISLLGFKFSIILIAVPLIGVGFPLLFSRKQNLKFWGGFLIGFSLLFIGLEFLKNSVP
                     RVDQNPEMFAFLSNYTDMGFLSILLFLGIGTLLTIIIQSSSATMALTLVMCNQGWISF
                     EIAAAMVLGENIGTTITANIAAIIGNVSAKRAARAHFFFNVIGVIWMLLIFYPFTRTI
                     ANVLVDITGKSPYTDTVEIPFALSIFHSSFNLINTFLLVWFTPLLVKLVTWLTPSKKE
                     EDEEFRLVHINAGLMTTAELSLVQAHKEVITFAKRTHRMFGFVRQLFNETNDKEFDNI
                     YQRIEKYEGISDRVEVEIATYLNKVSTYELSDESSRKLQALFRIISEIESVADSNFSL
                     ARTLKRKREQKVWFNQEMRDNLNKMFDMLDEAYNIMKENLEISQRSVSINLGPVYEVE
                     SKINEFRIFLKEDHIRSIEANKYKYQAGVIYSDLFNEAEKMGDYIINVSEAIAEIEKP
                     VKHKSYPIGK"
     gene            complement(32737..33615)
                     /gene="atpG"
                     /locus_tag="PLW22_01845"
     CDS             complement(32737..33615)
                     /gene="atpG"
                     /locus_tag="PLW22_01845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOU73474.1"
                     /translation="MANLKEIRTRIASVASTKKITSAMKMVSAAKFKKAQINVLRFRP
                     FNDQVNNILQSSIEFIGSLSGDYVNERQQVKNIVLIVITSNNSMCGAFNSNIIKYSIE
                     QYKSISARHPSARVTFYTLGKKGHEALSRRGYNVIELNHKMLDKPNIENTKDIFNTLV
                     NEFKHGSADKIFLIYNMFKSAASQQQVAEQLLPINILRTAKKTNKIKPIIEPNPQVLV
                     ERILPYFLLNKIHSAILESVAAEHGARMTAMHQATENAVALHNQLLLEYNKARQAAIT
                     KEILEIVSGANALSSK"
     gene            complement(33625..35211)
                     /gene="atpA"
                     /locus_tag="PLW22_01850"
     CDS             complement(33625..35211)
                     /gene="atpA"
                     /locus_tag="PLW22_01850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOU73475.1"
                     /translation="MAGIKPSEITQLIKDELAGIETKARFEETGFVLQVSDGIARVFG
                     LNNVKSNELVEFESGVKGVALNLEEDNVGIVLLGPTELVKEGDRVKQLGEIASIPVGD
                     GLLGRVVNTLGEPIDGKGPITGKTIVLPLERKAPGVIYRQPVSEPLQTGIKAIDSMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQRNNYLKGDPVYCIYVAIGQKGSTVASIAKT
                     LEDSGAMDYTTIVVATASDPAAMQFYAPFAGATIGEYFRDTGRHALVVYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIIESDDIASMMNDLPSPLKPLIKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNNGIRPAINVGISVSRVG
                     GKAQIKSMRKVAGTLKLDQAQYRELEAFAKFGADLDAATLAVLDKGAKNVEILKQGQY
                     QPLPVELQVAIIFCGTKGLLKNIPTGKVRNFEKDFLERCQLLLKSELEQMAKGDLNDQ
                     ITDALTNLAEEVAKNYTEFK"
     assembly_gap    35224..35323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(35439..35933)
                     /gene="atpF"
                     /locus_tag="PLW22_01855"
     CDS             complement(35439..35933)
                     /gene="atpF"
                     /locus_tag="PLW22_01855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOU73476.1"
                     /translation="MGLVTPDYGLLFWALLSFGVLLFILKKFAWKPILQALKSREESI
                     ARSMRLAEQARAEIEKLQEESKLMAEKARQEREREIAETKALREKLLAEAKEQARIET
                     QKMLDKAREQILLEKEAAKKELYATVAELTISLTEKVLRAQLKDKEKQQEYIERLLDE
                     IPKN"
     gene            complement(35945..36202)
                     /gene="atpE"
                     /locus_tag="PLW22_01860"
     CDS             complement(35945..36202)
                     /gene="atpE"
                     /locus_tag="PLW22_01860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOU73477.1"
                     /translation="MNTLLILLQSIDGASIAKFGIALGAAIIVLAAGLGIGRIGSSAL
                     KSIARQPEASGDIRTNMIVSAALIEGVAFFALIILIVALFV"
     gene            complement(36221..37306)
                     /gene="atpB"
                     /locus_tag="PLW22_01865"
     CDS             complement(36221..37306)
                     /gene="atpB"
                     /locus_tag="PLW22_01865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOU73478.1"
                     /translation="MKKRINLSVLILLLLIAASINCAQADEVHKKDSKGFNTAEFIFD
                     HVSDSYEWHILTVNHHHISIPLPVILYSKKTGLHVFLSNKFHHDHGVYKGFRIETEGK
                     LKGKIVELNNRGEVDETNPLPYNFSITKNAFSILFTSILLALIFIGIARRYKKDPDSP
                     PKGLQNVFEPFIIFIRDEIAIPSIGEKRYNHFMPFLLTLFFFIWFSNLLGLIPLFPGG
                     ANVTGNISVTMALALFTFFTINRHANKDYWKHIVNPPGIPTFLKLPIPIMPLVEFIGV
                     FIKPFVLMVRLFANILAGHMVATVLLSLIFIFSSMIAWLGFAVAPISLVFLIFMSLLE
                     LLVALIQAYVFTLLTALYIGMATEEHH"
     gene            complement(37667..38047)
                     /locus_tag="PLW22_01870"
     CDS             complement(37667..38047)
                     /locus_tag="PLW22_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU73479.1"
                     /translation="MAKINEPEFNANNINLVGFGTEINGDIKSSGDLRIDGILVGNLN
                     VKGKVVVGETGKIKGELVCKNSDISGVVEGKITVDELLSLKSSARINGDITVGKLAIE
                     PGCKFTGYCNMLGMESKEVIPGQE"
     gene            complement(38093..>38599)
                     /locus_tag="PLW22_01875"
     CDS             complement(38093..>38599)
                     /locus_tag="PLW22_01875"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU73480.1"
                     /translation="TAPLIHSDFNHLINNTITIFILIAGLVYFYRDLWYKILSLSWLL
                     SGMMVWIAARTSYHIGSSGIIYAIASFIFFSGVFRKNINLMAISLLVVFLYGGLVWGV
                     LPIFPQISWEYHLFGGITGAILAFVYRRQGPPPQTWSWMSETADDAEETSEYWESEIF
                     DESDKEKL"
BASE COUNT        11555 a         7922 c         7702 g        11320 t
ORIGIN      
        1 catacgcttt accacaacaa cggcaagggt ctttttgcct gctggatttg ctgattttcc
       61 tttttccaat atctattttt caaaaggatt agaacgctaa tccctttagt tgacattcaa
      121 tcatttaatt ttttcaaatg gaaaaagggt taatttttga tattcgacat ttttcggttc
      181 acgacggtcc tggaattaga actactgttt tccttaaggg ttgtccactg cgctgtgttt
      241 ggtgccacaa ccccgaaagt caaaaatatg aaccggaaaa ggttcaacga acaaccaaac
      301 tcgatggtaa ggaatacaac tcatccgaaa ctattggtag ttacatgact gccgaagagg
      361 tttttgacca agtaaaaact cacatcccaa tattcgacga atcgggggga ggggtaactt
      421 tctcgggtgg cgaaccgcta atgcaaccaa agtttttagt taatatcctt aagctttgcc
      481 gggcaaatga tattcacaca gcagttgaca cctgtggctt tgcattgcat gaggtcttta
      541 acttgatttt accctataca aaccttttcc tttttgactt gaagttgatt tcgccccaaa
      601 agcatgaaag ttacaccggg caaacaaacc atgtaatatt caaaaacctt ttcttacttg
      661 agcttaataa cgccccaaca ataataagaa tccctcttat tccaggaata accgatgacc
      721 aaaacaatct tgaggccatt aaatcagtat taaaaccatt gaggtgtgtt aagcgtgtcg
      781 atttactacc ctaccaccat atggctaagg ataaataccg ccgtatgaat ttaccctata
      841 agctagaaga tcttaagtca tatactgcca atcaacttgg cgaaattaaa tcattcttta
      901 atgatataga tattcctgta acaattggag gttaaacaca acaaagtctt acattatgat
      961 gacagaacga attcagcaac tccgcaaaca aagtctcaat gctgtaaaca cactttcagc
     1021 tgagcgtgca ttgctggtaa ccgaatacta caaaaaggcc gatagtaatt tacctatacc
     1081 catccttagg gcaagggcct ttgagcatat catgttaaac aaaaagattt gtatcaaccc
     1141 gggcgagctg attgttggtg aacgcggacc agcccccaag gctgcaccaa cctaccctga
     1201 ggtttgcttg catagcctcg acgatttgga gattattaac tcccgcccta aggtttcgtt
     1261 tagggttgat gaagaaacac gtgaagttta tcgcaatatc attataccct actggacagg
     1321 taaaacctta cgtgaaaaaa tttttgcttg gctaagcaat gaatggcatc aggcctatag
     1381 cgcaggtgtt tttactgagt ttcaggaaca aagggcaccc ggacatacgg tatcgggtag
     1441 aaaggtattc caaaagggaa tgctcgacct gattaatgac atcgattcat cgcttaaatg
     1501 cattaaacaa gaagcaaata caagggaaaa ggttaatgag ctggaggcca tgcgaattgt
     1561 agcaaatgcc ataattggct ttgccaatcg ctatgccgat aacctacatc tactggctga
     1621 aaaggaaacc gatgaaaacc ggaaggccga actgcaaaaa atggaagtta tttgccgcag
     1681 agtgccaact catgctccac aaaactttca cgaggcgctt cagcattact ggtttattca
     1741 tgttggcgtt atcacagagt taaacccttg ggattcattt aaccctggaa gactcgacca
     1801 gcacctttac cctttttacc gtcgggatat cgaaaacggg accattaccc aggagcaggc
     1861 cattgagctt ctgcaagctt tctgggttaa gtttaacaat cacccatcgc ctcccaaaat
     1921 gggtgttact gcacaggaaa gtaacacata taccgatttt gcgcttatta atcttggcgg
     1981 ggttaaggaa gacggttccg atgctgtaaa tgagcttaca tacatcattc tcgacgttat
     2041 tgaggaaatg cgattgctgc agcccagcag catggttcag gtaagtaaga aaaaccctga
     2101 taggtttgta aagagagccg taaaaataac caagacagga ttcggacaac cctcgttttt
     2161 tagcaccgat gcaattgttc agcagctttt gagtcaagga aaatcgttgg ttgatgcccg
     2221 aaatggcggc gccagtggtt gtgtggaaac tggtgcattt ggaacagagg catacttcct
     2281 aactggttac tttaacctta acaaggttct tgaaatcacg ctcaataatg gcgttgaccc
     2341 cttaaccggc aaaaaaattg gtctggaaac cggcaatccg gaaaacttca aatcatttga
     2401 ggaattctac gaagcctttg aacgccaact taactacttt atcgacatta aaattgaagg
     2461 caacctagta gttgaaaaac tttgggcaat gaatatgcct gttccattcc tttcgctact
     2521 tattgacgat tgcattgcta acgcaaccga ctataacgct ggaggtgcac gctataacac
     2581 cagctatatt cagggggttg gactaggaag tatcaccgat aatctgactg caattagaaa
     2641 acacgtttac gaagatggta gcattggaat gaaagagatg ttgttcgcat tatcgtcaaa
     2701 ctttgatggt tacgatgaat ttcgctataa actgatttat gaaaccccaa agtggggaaa
     2761 caacgacgag tatgccgaca ggcatgcagt aatggttttt aatagttttt acaaggctgt
     2821 aaacggacgc ccaacatacc gtggtggggt atatcgcata aatatgttac ccaccaccag
     2881 ccatgtttac tttgggagca aaattggagc aatgcccgat ggccggaagg cttttgagcc
     2941 actaagcgaa gggattagcc cagttcaggg tgctgaccgc aaagggccta ccggagtaat
     3001 cctatcgtgc tccaaaattg accatattaa aacaggagga acgctactta accaaaaatt
     3061 ctcgccctcg tttttcagca ccgatgaaag ccttgataaa cttgtgatgc taatacgcag
     3121 ctactttaag ctcgatggac accacattca attcaatgtg gtgagcgcca ataccctacg
     3181 cgatgcgcaa aaacatccag aaaaataccg cgacctgatt gttcgcgttg ctggctactc
     3241 cgactacttt aacgatttgg gcgaggcctt gcaaaacgaa attatacgaa gaacagagca
     3301 tgaatcattg taaagtatta ttttagatga accttacaca aactgttatg aagcgaattt
     3361 tctcacccct cttgcttttg attgttattt gcttgttcct actgggtttt cagcggcaaa
     3421 atcctgttgc tgagcaagtt cttaaatcaa tacctattgt cgatagtttt aagttgattg
     3481 ataacgaccc cttgtttcaa acaacatacg aggtatggtt taacgttccg cttgaccaca
     3541 ataacccaaa tagcgaaaaa tttcctttaa gggcatacta ttcgcaccga agctttacgc
     3601 gtcccatggt ggtagtaatt gacgggtata ctatgtacac ctcccgcccc aatgaactta
     3661 cccgcattct ggaagctaat cagctaacca ttgaacatcg gttcttttcc aaaagccgtc
     3721 ctaaggattc cattccatgg agttacctta acatacgtca ggctgcagcc gacgcgcatg
     3781 ctgtaatatc ggcatttaaa ccccactacc aggggaaatg gatttcgact ggcataagca
     3841 agagcggaca agccaccatt ttccaccgca ggttttaccc aaacgacgtt gatgttagcg
     3901 ttccttacgt ggctccactt aacttttcaa gtgaagataa acgtgtttac gaattcctta
     3961 gcaaggtagg ctctgccgaa tgccgaaaaa aaattagaga atttcaaatt gcactcttta
     4021 ggaataagaa cactttactc ccaatgttcg agggccttgc tcaaaaaaac aattggcaat
     4081 ttacaatggg gattggtcgt gcttacgact taagcgtact tgagtatgag tttgcctttt
     4141 ggcaatgggg gtatagctgc tccgatatcc ctgcaggcaa ctcacagcct tcggttcttt
     4201 ttgagcattg gtctcaggta aatcccttta ccttttttga ggaaaaagag atagaaaagg
     4261 taaggccatt cttctatcag gcaatgaccc aaattggaat gtatggctat aacatagagc
     4321 cattcaggaa ctacctcccc gataaaaaaa atatcacctt tgagtttacc cttcctaagg
     4381 gtcacacggt gagcttcgac tcttcagcca tgctcgatgt ttacaggtgg gtaagggatt
     4441 cgggcaacta catgctctac atttacggac agaacgacgc atggagttca accgcagttg
     4501 accccggcac aaaaaccaat gcagttaaaa tggtttgccc gtcaggtagc cactcaaccc
     4561 gcattaagga tttcccaaaa ccgctgcgcg atagtatcta tatcaccctt gaaagatggc
     4621 ttgaaatgcc ggttggtaga aagtaaaaaa atgcaacctt taccgggttg cattttttct
     4681 gggggaaaaa ttactttttc ttaatcgctt cattgtatag ctccgataca atttcccagt
     4741 ttataaggct ccaaaagtta tttacgtatt cagcacgcct attctggtat ttaaggtagt
     4801 aagcgtgttc ccaaacatca atccctataa gggggatgcc cttaaaatcg gacacatcca
     4861 ttagcggatt atcctgattg ggagtagagc caattttgag ctttccattg ccatcgacca
     4921 caagccaggc ccaaccgcaa ccaaaacgtt ttagtgcagc atcgttcatt tgagcaataa
     4981 ggttttcaac tgaaccaaac tcatcggcta ttgctttatt tagggttggg ctcaaaccct
     5041 ttcgattccc aggttttagt atttcccagt aaagtacatg gttatagtat ccccctccgt
     5101 tattcctaat tccagcaggg tactcagaca cttttgcaaa aatttcatta agcgattttt
     5161 cggtcagctt taagtcacta gcctgcttta aaaaattatc gaagtaagcc ttgtggtgac
     5221 ggctgtagtg aatttcaacg gttagggcat caattgctgg ctctaaagct gcgtattggt
     5281 aaggcaattg gtaaaaattg agttccgata ccctttgtgt taaaagagta ttctgtgcca
     5341 ttgaaagtac tgatatcgct gtggctaatg aagtaataat tatcctattc atgattcctg
     5401 aattttttgt ataaacaaca cacaggagtg aaaagttcaa gaaaataagt gctataactt
     5461 aaacccaagt gccatgaatt aacgatttca gccagtctaa ttatagagga aggagtcgga
     5521 atatacagaa tatgatggag ttagaggaag ttagaggaat agtaaaatgt gaacggttaa
     5581 accaaactgc catggattaa caaatacaac caggctaatt aaaatgtgaa tacagaaaca
     5641 ttttattgga aattcgccaa ttgaaaaatt caatttttcg aattgactaa taaaaacaaa
     5701 tcattccgcg atctcggaat gaaagatttt gaaaagtcgg ggttatctcg ataagtcaat
     5761 agaagcagga tgtttaaatg ttttttgttt ttctattgac ttctcgataa aataacaagt
     5821 gcttaaaaga ctctaaaata gtaatgagag tttattttgg ccgttgttta cagatagata
     5881 tgcagaaaaa atagctctgg gtaaaatcaa ttaacccaga catcctaaaa ctcctgccct
     5941 ttttgctttg gataatcaat tgagtagtgt agccctctac tctcgtgcat ttcctgagct
     6001 gattttatta tgaggtaacc aacgttaatc atattccgga gttcgcatag tttttgggta
     6061 agaattgatt ttttgtagag ttcctcggtt tcgcggtaaa gtatttccag gcgagttaat
     6121 gctcgctgta agcggaggtt tgaacgaaca attcccacgt agctactcat aatctgctgc
     6181 atctcacggt agctctgggt gataagtacc atctcctcgt tatgaattgt tccttcgtag
     6241 tcccattcgg gtatagagtg gtttatttca tagctcttat agtgttccat ggaatgttgt
     6301 gcgccacgat gcgcataaac aattgcctca ataagcgaat ttgaggctaa cctattggca
     6361 ccatgtaaac cggttgatga gcattcacct actgcgtaaa gcctttttat ggttgattga
     6421 gcattagtat caactttaat accaccacac atgtaatgtg cagcaggaac aaccggtatc
     6481 atatcctttg taatgtcaat gccaagcgat aagcatttct ggtagatgtt tgggaaatgg
     6541 tcaattatct cctttggttc cttgaaggta acatcgaggt aaacatagtc gtcgccccta
     6601 accttcatct cgttatctat ggctcgggct acaatatcgc gcggtgcaag cgacccccta
     6661 gggtcgtact tctgcatgaa ttccttaccg tcgatggttt taagcacacc accaaaccct
     6721 cgcattgctt cggttataag gaacgagggg cgttcgcccg gattgtaaag cgatgtgggg
     6781 tgaaactgca caaaggccat attttcaacc tgtccctttg cccggtaaac catggcaatg
     6841 ccatcgcctg tggcaacagg agggtttgta gttgtgttgt aaatattgcc agttccgcca
     6901 gttgccaaaa cagttactcg gctcaggaaa gtgtgtacct cctgtgtttt aaggtttagt
     6961 acgtatgctc cgaaacatgt aatatcagga tttccgcgtt taacctcctg tccaaggtgg
     7021 tgttgggtga ttatttcaac agcaaagtaa ttctcgtaaa cggtaatgtt gggatgattt
     7081 cttaccttgt tgcttaaaac cttttgtatt tccgcgccgg tattatcctt atggtgtagt
     7141 atgcggtgct ccgagtgccc gccctcacga gccaaatcgt actgaccgtt gggctttcgg
     7201 tcaaacttaa cgccccagct aataagctgc ttaatctggg aaggggcttc attaaccact
     7261 atgcggacaa cttcctcgtt gcaaagccca tcgccacaaa tgagggtatc gtgtatatgc
     7321 ttttcgggcg tatcggggtc gtatgttaca gcggctatgc ccccctgtgc gtatgctgta
     7381 ttggtttcgt ctaatctatc cttagtaaca actataacct ttgcgtggtc ggctacttta
     7441 agggcaaaac tcatcccgcc tatgcctgac ccaattatta ggaaatcggt ttcaatcttg
     7501 ctcatcgggt taaaggtata cagcaataaa ttgccgtgtt atgtttgagc aaaactatga
     7561 attagtaggc tgttatcaaa gctaaatatt aattggtatc cgttttcgtt ggctaaaggt
     7621 aattatttcc ttgtatccta acgatttaag gaggttaacg gcctcatcaa agtactgccc
     7681 aatgttttgc tcaacatgag catcgctacc cagggtaatg ggtacccggt agtgatgaag
     7741 gatttcgaga aaatcgaccg atgggtaaaa ttcggcacag ggtttatttt ttccgctggt
     7801 attaagctca accaccacac cgcaatcgct aaaaatttgt gcacaccttt cgtaaatttt
     7861 ctgcagctta aacgtagggt aaactgcaaa tttttttgct aaatcggcat gaccaataat
     7921 atcgaacatt ccggtttgtg ctgcctcgcc aacaagtttg aaatatgtgc ggtaaaaatc
     7981 atcaatatca atgccttggt aaccgttaat atcggtatca aaattccaat catcaataaa
     8041 gtgaaccgaa ccaatggtat aatccaaggg aatagattgt aaaacacctc gggtttcatt
     8101 aaccaaattg ggaatataat ccacctcggc tccaaaccga atggttactg aattggattt
     8161 gtgctgaata cgatttactg tgtcaatcag ttcaggaact cttcccaaat ccattgccca
     8221 gccaataggt ttaagcgata tatgatccgt gaaacctatt tcgtaaatcc ctgatgcctc
     8281 tgccgcataa accatttcat cgtgtgtatg atgtccgtca gaaaagttgg tatgcatgtg
     8341 gtagtcgggg tacattttat atcgatttta gtttataaac aaatccaaac gtttcgtgtt
     8401 catatcaaat ttatcacatt tattgctatt attgccaaaa tttttgatga tttgattacg
     8461 gttgtaagca taatggctgg gggtatgttg cttggttttc ttttaagagc aaagcaacgt
     8521 attgtttccg gtaatgagaa gcttatcact tatgccatat acctgcttct tttcatgatg
     8581 ggcgttagca taggttcaaa cgaacaaatt atgaactcgt tgagtacgct tggtattgtg
     8641 gccctaatag tctcaatggg tgctataatc gggagcattc taacaggttt tttggtgttt
     8701 aaattgtttt ttaaaaacga ttaaagttga aagggagttt aaccatactt tcatttttca
     8761 cgctggggtt aattgttggt cgatatactc cagttaacga ttggcttagt tccgttgact
     8821 acagctccct atcgctttac ctgcttatgt ttttggtagg aattagtata ggttccgata
     8881 cccgatcgtt acaagctctc aaaagcatga atttaaaaat cctgctgata cccttagcaa
     8941 caattattgg tactgctatt ggggtttggt tcatttcaat ttttgtttca cgatactcta
     9001 ttaaggaact tatggctgtt ggagccgggt atggctatta cagcctatca agtataatta
     9061 tcaggaatag ccacagcgat gcacttggcg ttgttgcttt gctatcgaat gtaattcgcg
     9121 aaattattac cctgctgctg gcaccgctta tggcaatcta ttttggtaaa gttgcaccaa
     9181 tctgctcggg aggagccaca agcatggata ccacattgcc aataatttca agagcatcgg
     9241 ggaaagagta tgccattcca tcgcttattc atggaataat acttaccata ctggtgcctt
     9301 tcattgtaac gttaattcta tcaatctaaa taacaaagcc atgaaaatca aaactttgct
     9361 actggcagca tttgcgtttt tgctgttaat ctcctgtaaa acaaaaaccg aaaaggaaaa
     9421 ctttaatcag gatcatctga tttacgccac catttggtac caaaactccc ccgaaatgaa
     9481 agccctgtac taccaaggct tcaatattgc agaggaaaga ttgaaacagt acacgaaact
     9541 aaaaactgca aaacccaaag cagtggtggt tgatattgat gagaccatgt taaacaatgt
     9601 accattccaa gcgcaggaaa taattgataa caaagagttc tcgagtgaat tttggaacga
     9661 gtggagccaa cttgccaggg ccgaagccac cccaggtgcc atagagttca gcaagctttg
     9721 cgattcgttg ggcattccga ttttctacat cagcaaccga aaggtaaacg agtttgacgc
     9781 tacccttcgc aacctcgatt cgctgggttt tgcctttgca aaaccagagt tcataattct
     9841 cagggacaat gagagtagca aaaaagcccg tcgcgaaaag gtaatggaaa actatgaaat
     9901 agttttgctt attggcgata accttaacga tttttctgag gtttttgaag accgaagcga
     9961 tagctggggt acacccaccg tggagaaatt caaaaatgaa ttcggaagcc gatttatagt
    10021 tttgcctaac cccatgtacg gcgactggga aaaaaatata tatccgagtc gtggcatgct
    10081 gcctgcccag caggatagtg cacgccgctc tgtgttaaag ggctttaagt aagagcacca
    10141 attctctcac caaaaaaggg cgatttttcg ccctttttta tttagaaaaa atatcaatta
    10201 tgattaatca tccttttgtt tattaaccac ttgcattaat ttgctaattt tgaatgagat
    10261 tgctttatta agtatgtttt acatcaaata taaaatacta tgagaagctt agcccacctg
    10321 ttaatacctg cccttttcct gttttcatgt tcaaagtcaa atcatgaacc aaccgtttac
    10381 ttcaccaagg atattacacc ggaggggttc atgaaggttt acaaacagat ttccaaaaac
    10441 cttgaaggga aagtaggcgt aaaggttcat tttggcgaag aaggcaattc ctattacgtt
    10501 aaaccggaac tatttaagga tattgttctc gactgcaagg gaacattcat tgaaaccaat
    10561 gtattatata aaagtccgcg gcaaaaaacc gaaacccata ttgcgctggc caaacaacat
    10621 ggatttacct atgcccctat cgatatttta gatgataaag gggagcttgt aattgaaaat
    10681 gtacccggat gtaaacactt caataagttt tacttcggga aaaatattga tgagtataat
    10741 tctttcctca taatctcgca ctttaagggg catggatcgg ctggttttgg gggagccatt
    10801 aaaaatatat cgatggggtt tgcaacccct cgagggaaac tggccatgca caggaacaat
    10861 taccctgtat gcgataggga tcgctgtacc aactgcgata gctgcgcaca gcaatgcccg
    10921 gcaaatgcta tttcaactaa tcctatttgg attgatgtag ataaatgcac cggatgtgga
    10981 aaatgcattg atgtttgccc aaataaagct ttacgttacc ctaaaggcga cgtagatgtt
    11041 caaactgtat tctgtgaacg acttgctgaa tacgctaagg caatttacga ccgtcataaa
    11101 aagatggttt tcataaatgt cttagtcgat atatcaacat cgtgcgattg ctcccgaaac
    11161 cccggaaagc catttgtccc aaacattggt attttggcgt caactgacat tgtggccatt
    11221 gaaaaagcaa gccacgacct agtggctaaa gcccacggat gcgctgatcc atttctggaa
    11281 gttaaccgtg taagcggtta caggcaaatt cagtacgcta aaacgttagg catgggcaaa
    11341 atgaactatc gcctggttaa cctggacaac aaccaagaaa ctgtagtaat ttcaaagtaa
    11401 agtttctaaa ccaacaatcg caaacctgtt gcaaaaaaat aaattaagta atatctttgc
    11461 cgagcaattt ggccttatag ttcaagggat agaacggaag tttcctaaac tttagatcca
    11521 ggttcgagtc ctggtaaggc cactacaagg ggagttcttc cccttttttt attttcactc
    11581 caccaaacat gaggaaaaat atgtaaaagt ttgtaacatt tcttgtattt tttttatcat
    11641 tgcattattt ttataccaat tttaattaca tattgtaact tctatggctg aaacttacaa
    11701 ggttgatgca attgaccaaa aaatactatc gtacttggtt aagaatgcac gaatgccttt
    11761 tttggaaatt gctcgcgaat gtggaatatc gggagccgct attcaccaac gggttaagaa
    11821 aatggaagat gccggtatta ttgccggttc gcgaatggtt gttaaaccca aagcgctagg
    11881 atttgatgtt tgcgcttttg ttggggtaca gcttgtatca actaaccttt acgcaggagt
    11941 aattgaggct cttaaaatga tgcccgaggt ggtggagtgc cattttatca caggggaata
    12001 tgccctttta ctaaaaattt tctgccgcga taacgagcac ctgatggacg tgttggttaa
    12061 caccattcag aatatccccg gtatttcaag aacagaaact tttatttccc ttgaccaagc
    12121 cattgagcgc ccggtttacg ttaaggataa aagtgtgatt aaaggttaaa acaataatcc
    12181 atacaacaaa aaaaggctga accctaggtt cagccttaat tttattttat tttttagtaa
    12241 tcttcttctt cgtcataggc atcgtcatca tagtaatcat ccaaagagtc tttcaaatcg
    12301 taatcgtcca gatcttcaaa ctcatcaatc tcttcataaa aagagcgctt caaattcttc
    12361 tcctttttaa caggtttcaa ctttttggct ctctttaact cgtcggaatc gaaagaatcg
    12421 ttctttacgg gtttccgaaa atctttcttt gacttcatca caagacaaat taagatgttt
    12481 cctttgtttt ttaactattt agggttaaac attaaatata tcaaccgcaa ttttaagtat
    12541 tttttttttc gttgcaaggt attggcctac tgtatggtga aaatttttac gtttaaagca
    12601 tattatttta ccaagttaca catcagctat ataaaagtca atactttttc atcaaacaac
    12661 tatttttttt acataaacac atttacgtac atttgtgaag ttttaaaccg ctatatgggg
    12721 tccgaggtaa ttatccacca gttactaatt tttggcgttt taatggtagt gggcgctttg
    12781 ggctcctact tcggaatcat taaggacgaa gccaaagact tcctgtcgcg cttcattatc
    12841 gacattaccc ttccctgcct gattttttca acttttgcta aaattgatag taaccccacc
    12901 ctgcttatca acggtttact agtgtttggt tttacctttg ttaaccttgc aatcgctctt
    12961 tttgctggaa ccgtttcggc acgtctccag ggcttgaacc cagcaaacac tacggttcat
    13021 agccttcaca ccatgtttgg gaatattgtg tttttgggct acccgctctt tgatgctcta
    13081 tttccaggag gaattggaat attctatgca gctgcatacc aacttgcttc aaactcggtt
    13141 actttcacct acgggatata caggctaagt gctggtcaag caaaatcggg gttaaaaagt
    13201 ttgcttaaca tcaacacttt tgccttacta attggtttta caattactgc ctttggcata
    13261 aaaattccag cgttttttat cgatgccctg agtggattgg gtaaggctac cagcccccta
    13321 tcaatggttt acattggggc tttgcttatg gggttgggta ttcgtaaatc cgtgagccat
    13381 aaaagtattt acctgcttag cctcaataag ttgattttat taccagtttt tttgggcttt
    13441 atttacctat ggatattccg attttttaac ctgcaaattt cgagcgaagc ctttattgtt
    13501 cttgtgatgc aaatggctat gccctgccaa acaataatag tggtaatgag cagaaggtat
    13561 ggtggaaact accagcttgc tgcgggtaat ctgtttgtct ccaccatact gagtatagca
    13621 acattgccag ccatttatat ttatctgaac tttcttctga aatgattttt atttcacaac
    13681 cagctttact tcaatcgtaa actcgtcgta aatcatttta tcgcctaatc cctcaaagaa
    13741 ttttcctgat ccataacgaa tattgtactg ggtgcgatca actgtaatcg ttgatgtgta
    13801 cacatttccc tttttaatta cctcaaaggt gattggcttt gtaataccct tgatagtcaa
    13861 atcggtcgtt actttggcct tatcgccatt aaacttgtcc gacctaacat ttccaaggag
    13921 agcaacccgg tatgttccaa cactaaaaaa atcgtcagat tttaggtggt caatcaactt
    13981 tttattccat tctgggctct ctaaatcgtc acatgttatc gattccatat cgataacaaa
    14041 atttccgcca gcaatctggt tatctctgat aaccaatttg ccctctttaa gcttcacttt
    14101 gccgtaatgt tcaccagtta cttttttggc attccatttc agggtggaac tactcgcatc
    14161 ggcaataaac tcctgtgacc aaactgtagt actaatcagg agtgctgtta gtaatgttaa
    14221 aaatttcatg ttctttttgt ttaaagttac acggtaataa caaatcaata ttcaatttgt
    14281 tttttagtaa cttagcgggg catatttgtt tagtgtccaa tgagagttgt aatacagcgt
    14341 gtaagtcagg ctagcgtttc ggttaacgga agcataaccg gttcaattgg taacggtctt
    14401 ctagtactag ctggttttga acattccgat actgaagagg atttggaatg gatggttggt
    14461 aaaattgtcc gcctgcgaat atttaacgat gccgagggca tcatgaacct cagcgttatg
    14521 gatgtgaacg gtcaaatact ggttgtaagc cagtttaccc ttcatgcaaa aaccaaaaaa
    14581 ggcaatcggc catcgtacgt tcaagctgcc cccccggata ttgccattcc gctatatgaa
    14641 aagtttataa tgatgctgga gcacgcaacc agtaaacggg ttgaacgtgg tgtgttcgga
    14701 gcaatgatgg atgttagcct ggtaaattct ggcccggtaa ccattattat cgactcaaaa
    14761 aataaagatt tatgacacag ctcacactcg acgatgctca gaaactggtt gacaattgga
    14821 ttaaaaccaa aggggtacgc tactttaatg agcttaccaa catgactctg ctggtggaag
    14881 aggtgggcga gttagcccga attatggcca ggagatatgg cgagcagtcg ttcaaggaag
    14941 gggaaaccga tcatgatcct gctcccgaaa tggccgatat actgtttgtg ctgatttgcc
    15001 ttgctaacca aaccggcatt aaccttaccc aagcccttct tcaaagtatt gaaaagaaaa
    15061 ccaaacgcga tagcgaacgg caccagggta ataccaaatt gttgtaactt tgccaacaaa
    15121 atcacaatct aataatagaa agctatggac ttatcgttcg aagacaagtt taacattgag
    15181 gtcgaagctg ccgaggttga aaaagcaatc aataagttca aggatattgc tcagtcacgt
    15241 aaaggcgacg tagagattct aaagcgctgc tttagcatga ttgaccttac cacactaaat
    15301 acaaccgatg gctatgagcg aggcaagctt tttgccgaaa aggtaagcca attccctaac
    15361 cagtttccgg gaatgccaaa cgttgcagcc atttgtgttt acccttcgct tgtggcagca
    15421 gtaaaagaaa atctatctgc gcccagcgta aaaattgcct cggttgcagc aggttttcca
    15481 gcatcacaaa cattccttga aattaaactt tccgaatgcg aaatggccct tgagcaaggt
    15541 gccgatgagc tcgatgtggt gatatcggta ggttctttcc ttgaaggtga ttactttacg
    15601 gttttcagcg aacttcaaca aattaaagag gtggctgaga acgccaaggt taaagtcatt
    15661 cttgaaactg gcgcattacc aagccttgaa gacataaaac tcgcttcgtt tttagccatg
    15721 gaggctggtt ccgatttcat taaaacctca accggcaagt tagaacccgc agcaacccct
    15781 gaagcggttt acgtaatgtg tttagccatt aaggagttcc atgaaaaaac cggacgcaag
    15841 gtaggtatga agcctgctgg aggagtttca aactcctacg atgctgcgct tttctatact
    15901 attgttgagc aggttctagg tcaggagtgg ctttatcctg gactattccg gtttggaact
    15961 agtcgccttg ccgatgtact attaagcgat attctaggta aagatgtcaa gtatttcagc
    16021 acatcgtcat cagctaaata ctagataaat tctagttata tgcaatgaaa aagggtaccc
    16081 aaaattggta ccctttttta tttgaattat tagaaattac caccagatag cgtaaagaat
    16141 aatcaaaagg gctataatta ttgctgagcc tatcttaaat cctttacccg ttgagaagag
    16201 ttcctttgaa attgttatag cttttacatg ctgcttattc ccctcaacca tagcaaatat
    16261 ccagataatt gctaaacaga ttagaaaaac aattcccatc ctatccataa agggcaggtt
    16321 gggtagaagt aacttaaata gtagcgagaa cacgaatgtt ccaactgctg ctcctaaagc
    16381 accattggct gttgcttttt tgtagaaaaa tccggctagg aaaattgcta atgcgccagg
    16441 acttacaaat cccgtaaact cttgaatata ctgaaaagct tgatcgaggt ttgaaagcat
    16501 gggtgctatg aatactgcta ttagcaacgc aacgaatgct gctatacgac caactgtaac
    16561 aagtttatgt tccgaggcat ccttgtttat atattcttta taaaaatcca tagtgaatat
    16621 tgttgaaaca ctattcatca tcgatgccaa tgagcttaca attgccgcta ccaatgcagc
    16681 aaaagccaag ccctttacac cagtagggac aaagttgtga agtagccaag gatacgcctc
    16741 gtctgacttt gcaatatcgg catgtaatgc aaaggcaaca atcccaggga taactacaat
    16801 aagtggaagt aaaaccttta ggaatgcagc aaataccaat ccgctctgtg cctccttaat
    16861 gcttttggca gctagggcac gctgaataat atactggtta caaccccagt agtagaggtt
    16921 tgcaacccac ataccgccaa ttagtacgct aattccgggt aaatcaatat aggcatcctt
    16981 ttgtccaccc ttcccatcgg gtatcatcag tttgcctttt tccagaatca tatcaaattt
    17041 ttcgggagcc ttttggagta aggctttaaa tccagcaata aagccatggc catcgcttag
    17101 gtaatctaaa gcaaagtatg ttgttactaa accacctcct acaagaaaaa caacctgcac
    17161 aacatcggtc catgctacag cctttaagcc accataaatg gagtagagtg cagcaaaaaa
    17221 ggctaaacca ataatagcgt aggtcatatt tatacccatt attcgctgca tagccagagc
    17281 accaaggtag attatagagg taagattaac gaaaacaaaa accagcagcc aaaagccagc
    17341 cattacggtt ttaacacgtt tatcgtagcg gacctcaaga aactgcggca tggtaaaaat
    17401 cctcttctcc aaaaatatcg gcagaaagaa aacagccacc agaattagcg ttaaagctgc
    17461 catccattca tacgatgcaa ttcctaatcc aatggcatag cctgaacctg acatcccaat
    17521 gaattgttca gcagagatat ttgatgctat tagcgatgca ccaatagccc accagggcaa
    17581 cgccctaccg gctaaaaagt aatcccctga gttggtttcc gaatcgcgct tacgaaacga
    17641 aacccacaag cctaaactaa ctataagtaa agcataggct ataaacacaa tgtaatcgac
    17701 tgttgcaaac gacatatcag taaggttttg gtttaaggtt aaaagcaaaa gtataactaa
    17761 gagcaaaata tctagcacaa atgagctaca tcaatagtat cacactccta atttttacct
    17821 actccataaa ggtgatggat acactccttt ttccgttaac tctttaagtc ggttaactac
    17881 catgggtcta tcaccgggat atgtaacgcc aaaccactcc gctttggttt ctaatacctt
    17941 acagcgggca ataccctctt taatggctcg gtttacaacc gtgggaatgt aaaattctgc
    18001 cttaacattt ccaatttggt tgctgaaaaa gtcggtcata agcttctcgg tgtagccgaa
    18061 aaagtcagtg gtgaaacccc agaagttcat cgatacggga acgccctcat caacctttac
    18121 aaaattacca ttttcatctt tgtactttac ctcgccttca acacgctcaa tgtgagtgcg
    18181 ttcaacaaca tctaataagt atccattttt atcggttgag catactccgc gtgaaacagc
    18241 gccttgttcc gaaagggtgt ttccaagctt gtaaccaacc ataaagtagc ttcccttttc
    18301 cagattgggt ttggatagtt cctgagctag taccctaaag gtgtcgaaac catagaaatc
    18361 gtcggcatta atcacagcaa acggttcatt aattaaatga cgggttaccc atactgcatg
    18421 tgccgttccc catggctttt cgcggccatc aggaacttta aaaccctgcg gaagcatatc
    18481 gagttcctga aaggcaattt caactcttac atctgagtcg aaacggttac caaatacttc
    18541 caaaaaatcc ttttcaatgc tcttgcgaat tacaaaaaca actttcccaa aaccggcacg
    18601 aatagcatcg tacacggagt aatcaatgat tgtttcgcca ttgggtccta atgcatcaac
    18661 ctgcttcaaa ccaccataac ggctacccat gccagcagcc aaaataacaa gtgtaggttt
    18721 catatcttaa gatacttagt taaaattatt ctaaaatcaa ttttttgccg tattattaac
    18781 aactgattgg accaatttca aaacgcactt atctgctttc agtaaatttt tatgtcgttg
    18841 ttttgccatt tcggcaaagt tattgaatgg ttataaaaac aaaaacttta tcggccatga
    18901 aaaggtttat ctttttagtt gcacttctta atgttttgat aataagcaca aatgcacaga
    18961 ataaaaccaa aagtcagctg gaagccgatt ttgaaaaatt taagaaggag cgtgagcagg
    19021 attttcagga tttcaaacaa aagcgcgaag ctgagctaaa aaaaatggaa caggagtacc
    19081 tggactacta caatgaaatg actggactca agaagcatta tgttgagaaa aaggaacccg
    19141 aaaaggctca aaccgttcag gatatcattg attacgaaaa cacgattgct aaaacattgg
    19201 gatacaaaat tcctgaaagg gtaaaggaaa aagtggaagc caatactaag tccgaagttt
    19261 caaaaaagga agaagctaaa acagaagctg agaatgtaaa tgttgaagat aaaaagcccg
    19321 aagtgtcgtc atcaacattt aaaccagagg ctggcactcg cgatggaata ccagtactta
    19381 cacctttacc caaaagacta gccaaaatta catcgccttt cggtgtaaga attcacccaa
    19441 ccctaggccg gcctattaaa cataatggtg tggattttgg ctcgggacgt ggggctgagg
    19501 tttatgcagc atcgagcggc aaagttgttc ttgcccagta caacagcacc tacggaaact
    19561 atatcattat tgaacataat gatggaacaa gttcggttta tgcccatttg gataaaataa
    19621 aggtttccaa ggggagccct ataagaaaag gcgatgttat aggttatact ggcagtactg
    19681 gtcgaagcac tggaccacac cttcattatg aggttcgtat aaagggtatc ccagttgacc
    19741 ccaagggata tttggttgaa accaaatagg gttcaacttt aggaaaaagg attcacaatg
    19801 ccctaaacaa ttttaaatta gggtaaacac taactaattt tcctattttt gcaaaaaatt
    19861 tgcaaatgct aaaacgcgag gacattgagg ttatggctcc agcgggcaat tttgaatcgc
    19921 tcatggcagc catacaggga ggagcaaatt cggtttactt tggtgttgga aacctcaaca
    19981 tgcgcgctaa atcgtcaata aattttactg tggacgattt accggaagtg gttcgcattt
    20041 gcaaaaagaa caaggtaaaa acatacctta ccgttaacac ggtaatttac gatagcgaca
    20101 ttccttacat gcaccaaatt atcgacaccg ctgccgaagc aggtgtcagc gcacttatta
    20161 tctccgacca ggctgccatg ctttacgccc gcaaaagggg aatggaagtg catatttcaa
    20221 cacagttgaa tattagcaat acagatgcac ttaagttcta ctcacagtgg gctgatgtag
    20281 ttgttttagc ccgagagcta aatcttgaac aggtaaaaac tatttaccaa aacattcact
    20341 cgcagaatat tactgggcca aatggcaatc ccataaaaat agaactattt gctcatgggg
    20401 cgctctgcat ggctatatcg ggaaagtgtt acctaagctt acatgaggcc aactcgtcgg
    20461 caaacagggg agcttgcctt caaatttgtc gccgttcata caccgtaacc gataatgaaa
    20521 ctggtgcaca gctggagatt gacaatgagt atataatgtc gcccaaggat ttgtgcacca
    20581 ttaacttcct ggataaaatc cttgatgcgg gcgttagagt tctaaaaatt gaaggccgtg
    20641 cccgaggccc tgaatatgta aaaacggtaa cacaatgcta taacgaggcg gttacatcgg
    20701 tttgcaatgg aacctatacg cccgaaaagg ttgaaaattg gatgcagcgc ctttctacgg
    20761 tatttaaccg cggtttctgg gatggatact atcttggccg caggcttggc gagtggagca
    20821 aggcttatgg caacattgcc actgaacgca aacagtatgt aggaaaaata accaactact
    20881 tctctaggct gggtgtagct gaaataaccg ttgaggcctc ggagctttac ctcaacgaca
    20941 aaattctaat tattggccca acaaccggcg ttattgaata tgaggtaaag gaacttcagg
    21001 tcgatgaaaa accagcagag ttttcaccaa aaggggtaaa atgttctatt ccggttccag
    21061 ataaagtgcg cagagccgat aagctttacc gaatagtaaa aatgaattcc gattagtaaa
    21121 atatttgtaa attagatgtt taattaaaac tcctattgat atgagcttca gccaactttg
    21181 caacaagatt ttttgggact gcaccctgca ctatcacacc atggataatg ttgatgccca
    21241 acctaacatt ccttaccagc caggaaccat tgaattctcg ctttttacaa agaactggat
    21301 tgatgccgtt cagtggcacc tcgaggatat catccgcgat cccaaaattg atccggtaga
    21361 agcgttaaaa attaaacgcc gtatcgataa atccaatcag gaccgaaccg atttggttga
    21421 aatgattgat agctactttc tggaaaagta taaggatatc aaacctcaat ccaacgccac
    21481 tataaacact gaaagtccgg catgggccat tgaccgcctc tccattcttg ccctaaaaat
    21541 ttaccacatg gagcaagagg ttaaacgaac cgatgtttca aaagagcata ttgacagctg
    21601 ccaaaagaaa ctaaacaccc tgcttgagca acgggtcgat ttatcaacag caatcgatca
    21661 gctactcgac gatattgagc acgggcgcaa gtacatgaag gtttacaagc agatgaaaat
    21721 gtacaacgac ccatcgctta atcctgtact atatggagca aaacagcaaa aaggttaacg
    21781 gatatcctaa aaagctactt gtggttaggc ttagcgccat tggcgatgtg gccatgaccg
    21841 tacctgcaat ttggtcattg gttaaaaatc accccgatgt gcaggttacc tttgtatcgc
    21901 aaggatttgc ccgcgatatt gttaaccaaa ttcctggagt tttatttgtg gaggcaaata
    21961 ccaaaggccg acacaagggg ttgttgggaa tttggcgatt gttcaccgat ctccgtaaaa
    22021 tagataggta cgatgcttta gccgaccttc acaatgttat tcgtagccaa attctccgaa
    22081 acttattttt actctcgggg ctaaaaaaaa tatccgttat aaataaaggg cgaagcgaaa
    22141 agcgcaaact tgtaaggcta aagaataagc ggctcgctcc acttaaaact acccctgaac
    22201 gctatatgga tgcgctcctt aggctagggt acacaatcaa tgatgagttt gattatcttt
    22261 tccgcgaggc tcgattaccc gaaaacatta caatccacct tggatacaag gacaagcggt
    22321 ggattggtat tgcgcctttt gccaaacata aggggaaaat ttacccgctc gacaaaatgg
    22381 agcgagttgt tgaaatcctt tccgcatcaa aaaccaataa aatcattcta ataggtggag
    22441 gtggaaaaga gcaagaaata cttgagggct gggagaaaaa atatccaaat accatttcca
    22501 ttgcccgaaa gtacagcatt ctaaatgaac taaagataat ttcgaacctc gacgtaatgg
    22561 tttgcatgga ttcagcaaac atgcattttg catcgttagt aaacaccccc gttgtaagca
    22621 tttggggtgc tacacatccc tatgcaggct tttacggatg gaagcaaaac cccacaagtg
    22681 ccgttcaggt tgacctttac tgcaggccat gctccattta tgggaataaa ccctgtttcc
    22741 gaaaggatta cgcttgctta aaccttattg agccagaggt ggtggtgagt aaaatcaaca
    22801 cggtaattga ccgtttcaac agaatttaaa attaaaataa ttatgactta cgtaaccaaa
    22861 gaaaagctat tcagcaggga atggtttaaa gcatatgctt taatactggt tggatcgttc
    22921 attcttgcag caggttttgt tctattcatc gacccacacc gaattgcccc tggtggtgta
    22981 tatggtattg gtattattgt ccattacctc acaaaaggaa tgttctcttg ggcacccgat
    23041 ggttttccta ttggtactgt tggtttgctg ctaaatattc cacttaccat tattggtata
    23101 agggttttag gcccaatgtt tggaattaaa acggtagttg gttttgtact ttcatcggtt
    23161 tttatcgata ctttacacct ttggcttgga tacgaccccc ttgttgacga tatgctgctt
    23221 tcctcaattt ttggcggtgt actcattggc tttgggttgg gcttaatctt caagtctaaa
    23281 gcaacctcgg gcggttccga tatcatagca atgataattg ctaagtatac ccgactaccc
    23341 ctaggccagc tgatgattta cgttgattca accattgtac ttttagcctt ggtagctttt
    23401 caggactgga aaattccttt atactcctgg gtggtaattt acgttaccgg taaggtgatt
    23461 gatatgacca ttcaaggggt tagctacgat aaagcccttt tcattattac cgataggcac
    23521 gaagaagtta aaaacaagat tattcacgat attgaacgcg gcggaaccat tattaacgtg
    23581 caaggtatgt acaccggaca cgataagaaa atgattttca caaacgtatc gcgtcgcgag
    23641 gttcatatgc taaaggatta catccagcaa attgacccga atgcttttat tactgtaatt
    23701 gatgccaatg aaattcttgg aaatggtttt aaaccgttaa acgagagtat gtagcttttt
    23761 acacatggaa aagggattgg tgttaaagag taccggaagc cattacaggg ttcgattgga
    23821 tgatggccgc gtggttgagt gtgtaccacg cggtaagctt aggcttaacg agataaaaac
    23881 aaccaatcct attactgttg gcgactgggt aagcgttgag cttaacccca acggggtagg
    23941 tttaataacc gccatccaag agcgaaaaaa ttatattatt cgtaaggcca caaacctatc
    24001 acgcgaggct cacatcattg ctgctaatat cgatcaggct ttactggtgg ttacaattac
    24061 tcagcccgaa acccaactgg catttattga tcgttacctg gttaccgctg aagcttaccg
    24121 cattcctact gttattgtct tcaataaaat cgacttagtt gattcagcca ttcaacccgt
    24181 gctcgatagc tacatctcaa tatacgaacc cattggatat aagtgcctaa aagtttctgc
    24241 caaaacaggt caaaatctcg atttacttaa acaaatactt caaggaaggg taagcttgct
    24301 atccggaaat tcaggggtag gtaaatctac gttaattaac ctcattgagc ccggtttgaa
    24361 tcttaaaacc gctgaaattt caaatgctca ccttaagggt aggcatacta caacctttag
    24421 cgaaattttt gagctaagct ttggtggatt tattattgat acaccgggaa ttaagagttt
    24481 tggtttggtt gacattgaaa aggatgagct ataccatttc ttccctgaga tatttaagct
    24541 atccgacaaa tgtaagtact acaattgcac ccatatccat gagcccggtt gtgctgttat
    24601 tgaggctgtt gacaagagcg aaattgcacc atcgcgctac ctgagctact tgagcatata
    24661 cgacgatgaa aacgaaaaat atcgacccaa attaaagtaa tagatcatta gtatgtttat
    24721 taataactta aatccaattg cttatgaaat cgcacgaaat tgactataga attattggtg
    24781 aatccattca attggttgaa attgagcttg acccacatga aactgtaatt gcggaagcag
    24841 gtgcaatggt ttacatggaa agtggaattg attttgaaac aaagcttggc gacggctcaa
    24901 atcccagttc agggttttta gggaaactgc tttcggctgg agcacgtgct ataaccggtg
    24961 aatcgatttt tatgactcac ttcactaacc atggtattgg caaaaggaaa gttgcttttt
    25021 ccgcacccta ccccggcact gttgtgccaa tcgatttgtc aaaggtgggg ggtaatctta
    25081 tagtccaaaa ggacgggttc ctttgtgcgg ctcttggtac aagtatttcg ctccatttca
    25141 accgaaaact gggagcgggc ttggttggtg gtgagggctt tatccttcag cgcctcagcg
    25201 gcgatggttt agcctttgtg catgccggcg gaaccgtgat tgaaaagcag ctcaacaatg
    25261 agaccttacg aatcgatacc ggttgcattg ttgcctttga gccaacaatt gatttcgatg
    25321 ttcaaacaag cggaaatcta aaaagcatgc tctttggcgg cgaaggttta ttccttgcca
    25381 ccctttccgg ttccggtaag gtttggcttc aatccatgcc tatcaagaaa ctggttcagg
    25441 ctcttgctcc ttacggcaga aatagaggaa aagaaaatag ttcaatactt ggcaatcttt
    25501 tagaagatta aaaatggctt caaccattag tatcgataaa tttattgaac tatcagatat
    25561 ttaccctgtt gttgatgttc gctcaccttc ggaatatgct cgtgggcata ttccgggcgc
    25621 atttaatatt cccctttttt ccgacgagga acgcgctgct gtaggaacta aatatgtaag
    25681 ggaaggacga caacaagccg ttagactggg acttgaactg gttgctccta agctaaactg
    25741 gtttgttgat tcattaagtt caatggttaa ccaaaaaaac attctggttt actgctggcg
    25801 tggtggaatg cgaagtgcca gcatgagctg gctttttggc ttagctggtt ttaagccctt
    25861 tacgttggaa ggtggttata agtcattcag gcgatacgca caggctgctt tcgacaagaa
    25921 atataaattt attgttcttg gcggcatgac tggctgcggt aaaaccgaac ttcttaaaaa
    25981 actcaaggaa aatggtgagc aggttgttga tctggaaggg cttgccaacc acaagggttc
    26041 tgccttcggc tggataaacc agccgcaaca acccaccact gagcactttg aaaacctact
    26101 ttttaatgag ttacgaaagt taaaccccgc actacctatc tggattgagg atgagagcaa
    26161 aagtattggt tcaattttta tacccctatc tatttataac caaattatta gtagcccaac
    26221 cattgttgtt gagactgacc taaaaaacag aatcgacagg ttgattaacg actacactgc
    26281 atgcgataaa caggacttaa taaactcggt tgtgcgaata caaaagcgcc tggggttaga
    26341 aaaggcaaaa cgctgtattg agtatatcga gtctggaaat ctgcgcgatg ctattgccat
    26401 tacgctggaa tattacgata agacctatcg ttacggaatt gagcaaaaga aaataccacc
    26461 aaagttttta acctttgatg gtacaagcaa cgatatgatt gttaagctga cgtcggttgg
    26521 taaaagcatt acacacccag cgttcccgga ttaatgcaaa gtactgggat tttggcttta
    26581 ttagctatta cgtattgctc ctgggctcca aaaacaaaat cggttagatc gatattaggg
    26641 gttgtcatca caataatcaa ttcggcctca atatcggttg ccaggttaac aatttcctcc
    26701 tgaaaacttt taccctttgc ggcattatga atttcccagt ccagaccatt ggattctata
    26761 tgcattttgg caaaagatag gttattgtta atttttttct gaacgcctga atcgaaagca
    26821 tttggaacaa tcacatggat cttggaacca aacagcttag caaaacgaat tccccattga
    26881 attttctctt tatcctcatc cctgaactca acgggcataa caatatgtga aaatcccttt
    26941 gccatatcgg gctcatcctg aacaaccaag aaaggcacct cggagcctgc tataaccttt
    27001 agcgcccaac ttccagtgag cttttgtact cctttaattc catgagtacc cataattacc
    27061 atttcggcat ttatttcact ggcaaactcg cttatggtat caaaaatatt gccctctaaa
    27121 aggtgagttt taacctcaac accatactca tttacaattc tttgctgatc gtccttaagt
    27181 ttttggcttg catcatcttc gctaaattta cttttattaa acaagccgcc ggtttgaata
    27241 acatggacaa ggttaatttc aatgggttcc ttagcaagct tcatcagttt tattgcgtgc
    27301 tttaatgcat tttctgcaac aggggtaaaa tcccaaggaa ttagatattt gttaatattt
    27361 tctgccatag gttttacatt ttagtactaa aaattgggta gttaaattta caaaatttta
    27421 atttaaaatc aatcctttaa accaatattc aattttttcg gccaattaac cttgttaata
    27481 tttgattaag tacatctttt ctggattcgc ttacattaag cttatttatg gcctgcgtgg
    27541 cattgtcaaa gtatgtatcg ataatgctta ccatttcatg ctgaacttct gctttactgt
    27601 aataatccat taccctgcca atctttagct gatcagtcaa tgatttactg ttgtatagtt
    27661 caatgaaatc gcttctttgt gttcccttta gcttttccgc agtaataatg gttaacatgg
    27721 ttttttttga tgcaacaatg tcgccaccaa tgcgtttacc aaaagtactt gaatcgccat
    27781 aacagtcaag cagatcgtcc tgaagctgaa aagccaaccc catcgacctg ccaaactcac
    27841 caataagctc tacatcattc ctgcctgcgc cgccaattac tgcaccaatc tgcagagccc
    27901 ctttaagtaa tattgatgtt tttagctcta tcattttaag gtactcatcg cgagaaacca
    27961 tggtgtctga ctcaaaatcc atatccattt gttggccctc gcatataccc atggcaaaag
    28021 agttaaaaat gttaatcacc tcgggcaaaa tcgacttatc aacctgaacg agcaacttgt
    28081 aggctagcac tgtcatggcg tcacctgaaa gaattgcaat attggggttc cacttcttat
    28141 gaatagttac caaccctcta cgcatatcag cattatccat aatatcgtcg tggaccaagg
    28201 taaaattatg gaaaagctca acggcgagag ctgcaggtaa tgcttggtca atatcatcag
    28261 agtaaagatt acacgctgca agtacaaggg ctgggcgaat tcgtttacca cccatactca
    28321 ttatgtatgc gattggctcg taaaggtttc ttggttcacc tgttaaatca agctgcttaa
    28381 ttgctgagtc aactttctgg tttattgatt caagtgtata cattctggtt gatttttact
    28441 cttcgtcgtc gaaaccgcta ctcatttcca ttacaagttc ttcctcctca ggttttaatg
    28501 gcttgggttt atcaaattga cgtaaaaccg agggaagtac aaataggttt gtaataacgg
    28561 catacagcag tgttatggca actaaaagcc caagtgcttt tgtaccacca aaccgtgaag
    28621 caataaacac actaaatccg aaaagtaata ctattgacgt ataaatcatg ctaatacccg
    28681 tttcgcgaat tgcatgaaat attgactctt tgatattgaa cttggttaaa tacaattcct
    28741 gacgatactt ggtaagaaag tggattgagt tgtcaacagc aatacccaaa gcaatactaa
    28801 atacaataac ggtgcttggt tttaggttta tacccagaaa acccatggtg gcagcagtta
    28861 gtattagcgg aataagattt attccaatgg caaaaagtac cattctaaac ttacggaata
    28921 gccaagctat ggcaagggca ataagtatta ttgcaaaaat taggctcgat gataaactat
    28981 ggactaaata cttaattcca taggtagtaa ttaggcttgc ccctgcaata tgaacatttg
    29041 tatgctcatc atcaaaaacc tgcctaatat cactgtttat gctgtcaaac aaaatgctca
    29101 ttttttcaga accaatatcg gccacattgt acattatcct tacatactgg ccggttgagt
    29161 ctgtcattcg ggcataaaca tcactaacac cgatgcttcg aggaacatac gacattataa
    29221 aagctcgttc aaagggtccg ggtagccggt attggctctc gttgccgtta tagtaggctt
    29281 gtcgggcaaa ttttgctatc tcggctatag aaaaaggttt tgaaagctct ggataagagc
    29341 taagtttttc ctgtaatctt tctacttttt ggtaaacggt atagttttgg taaccattag
    29401 gttttccagt gttatatact atttcaaggg gcataatccc cgtaaaattt ttttcaaaaa
    29461 atttcaagtc gcgctgtacc gggtgatttt gggggacatc atcaaccatg taaccgttag
    29521 ccttcaagag ggaagcacca aatattccaa taaccaaagc aaaaaccgta atggcaaaaa
    29581 cccatttgcg cttgtaatag gtggttaata ctatacgtga aactgcctgt ttaaaagtat
    29641 ttcgattaag atgacttaag tgtcgagctt tgggtgtagg gcttatgcta aaaaagaaag
    29701 gtataacaat taatgaaata agaaaaatac ctaggacgtt taaaaaggca atcaaaccaa
    29761 actcctgtaa aatttgtgtt ttagtgaaaa caaatgtcac aaaacctaat gcggtagtta
    29821 aattggttaa aaacgttgca ctaccaattc ggtatataac ccggtaaaga gctttgattt
    29881 tatggcggtg atgggctatt tcatgatggt acttgttaat taggtagata caattgggaa
    29941 cccctatcac tattaacaat ggcggcacaa ctgcagttag caatgttaaa tcgtaaccga
    30001 aaagggtaag tgaacctaac gaccatatta cggcagtacc cactaccacc aaggaaataa
    30061 atagcacgcg gaatgagcgg aaaagcacta aaagaataat tgcagtaacc agtgcagcaa
    30121 ggccaacaaa taggaacatt tcgtatttaa tcatttcggc caccactgct cttatgtaag
    30181 gtaagccaga gtagtggagt gtgtttccgg ttctagcttc gtattcttgt gatgcttcct
    30241 ttacctggtt tatgatttta atgcggtcta ccctggtcat cttttctctg ttcagggtta
    30301 taagcattag gtaggtgttg gtttcagcat tataaagcag ccccttatag aatggtaagt
    30361 ttttaacctg attactaagg gaatccggac taatcgtatt aaggttatcc agcaactttt
    30421 ttgctacgaa cttcctggta atagtatctt tttccagcgt tataatatcg ataacagaga
    30481 aaacgttttc tacaccatct acttgcttga ttttattgga caaattctcc caaagcaaaa
    30541 ctctatcgct actaaaaaag gctgaatccg ttaccccaat aaccattata ttctcaccct
    30601 tcccaaaaag ttttgccagc ctctcatttt ggatatatat gctatcggtt tttggaacaa
    30661 gggggatggg tttataggat agccttacct tggttgcttt ccatcccatg aaaaaagtaa
    30721 ataccagtaa aattgataca aggataaacc gtctcttaag aataaatcgg gcaagcttat
    30781 gaaacattgc tttactatta ggattcaatc agcaatatta gaaataatat ggatatgttg
    30841 ttgcaactat agctaatcaa tacataaaat attgattttt gatttatact ggtaaaagtt
    30901 acctttgcaa aaaattggtt gcgattttta attatttcta gttatttaaa ccaaaatata
    30961 ccaatgaatt acggacttct tgatcttttg aacttaattg gttcgctggg actattcctt
    31021 tacggaatga aaatgatgag cgaatcgctc caaaaggtag caggcgataa gatgcgtgcc
    31081 atcttgagtt ccatgacatc caacaggttt atgggaatcc taacaggctt tcttgtaact
    31141 gcagtaattc agtcgtcatc agctacaact gtaatggttg ttagctttgt taacgccggt
    31201 ttgctatcgt tggttcagtc tattggcgtt ataatgggtg caaacattgg tacaaccatc
    31261 acggcatggc taatatcgct tttgggtttc aagtttagca ttattcttat agcagtacct
    31321 ttaattgggg tgggtttccc tctccttttt tcccgtaagc aaaacctcaa gttttgggga
    31381 gggtttctta ttggcttctc tttgcttttt attggtttgg aatttcttaa aaattcggtt
    31441 cctcgagtag accaaaaccc tgagatgttt gcattccttt ccaattacac cgatatgggc
    31501 tttttgtcga tactactctt tctgggtatt ggtacacttt taaccataat aatccaatcg
    31561 tcgagcgcaa ccatggcgtt aacgctagtt atgtgcaacc aggggtggat ttcatttgaa
    31621 attgcagcag ccatggtgct tggtgagaat attggaacaa ctatcacagc aaatattgcc
    31681 gcaattatcg gtaacgtttc agccaaacgc gcagcaaggg ctcacttctt ttttaatgta
    31741 attggtgtaa tttggatgct gttaattttc tacccattta ctcgaaccat tgctaatgtt
    31801 ttagttgata ttacaggtaa atcgccttat accgatacag ttgagatacc ctttgctctt
    31861 agcattttcc actcatcgtt taatctgata aacaccttct tactcgtttg gtttacacct
    31921 ttgctcgtta aactggtaac atggctaacc ccttcgaaaa aggaagagga cgaagagttt
    31981 aggttggttc acatcaatgc cgggttaatg actacagccg agctctcatt ggtgcaagcg
    32041 cataaagagg ttattacttt tgccaagcgc actcaccgaa tgtttggttt tgttcgtcaa
    32101 ctttttaatg aaaccaacga caaggagttc gacaatatct atcagcgaat cgaaaaatac
    32161 gaaggaataa gcgatagggt tgaagtagaa attgccactt accttaataa ggtttcaacc
    32221 tacgagctta gcgatgaaag ttcacgtaaa ctccaggctt tatttagaat aattagcgaa
    32281 attgaatcgg ttgccgatag caacttttct ttagcccgaa ccctaaaacg caagcgtgaa
    32341 caaaaggttt ggtttaacca ggaaatgcgc gataacctaa acaaaatgtt tgatatgctc
    32401 gatgaggcat ataacatcat gaaggagaat cttgaaatta gccagcgaag cgtttccatt
    32461 aacttaggcc cagtttacga ggttgaaagc aagatcaacg agttccgtat ttttcttaaa
    32521 gaggatcata tcagaagcat tgaggccaat aagtacaagt atcaggctgg tgttatttat
    32581 agcgatttat ttaacgaagc cgaaaaaatg ggcgattaca tcataaatgt ttctgaggca
    32641 attgctgaaa ttgaaaaacc tgtaaagcat aaatcctacc ctatcggcaa atagtttaat
    32701 tagctgatat aaaaaagcgg gatgtagccc gcttttttac ttactgctca atgcgtttgc
    32761 acccgataca atttcaagaa tctccttagt aattgcagcc tggcgtgctt tattgtattc
    32821 aaggagtaac tgattgtgta atgcaacagc attttctgtg gcctgatgca tggctgtcat
    32881 acgagcaccg tgttcggctg caacgctttc aagaattgcc gaatggatct tgttcaaaag
    32941 aaagtaaggc aaaatacgct caaccagaac ttgtgggtta ggttcaatta tcggtttaat
    33001 tttatttgtc tttttagctg tccttaaaat atttattgga agtaactgct ctgctacttg
    33061 ttgctgcgat gcagcacttt taaacatatt gtaaattaga aagattttat cggctgaacc
    33121 atgcttaaac tcgttaacca gggtattaaa aatatctttt gtgttttcaa tattaggttt
    33181 atcaagcatt ttatggttta gctcaattac attataaccc cttcggctta gtgcttcgtg
    33241 tcccttttta cctaaagtat agaatgttac cctggcactt gggtgtctag ccgatattga
    33301 cttatattgc tcaattgagt atttgatgat atttgaattg aatgctccac acatgctatt
    33361 gttggaggtg ataacaatca aaacgatatt ctttacttgt tgcctttcgt ttacataatc
    33421 gccacttaat gaaccaataa attcaattga tgattgtaat atattgttta cttggtcgtt
    33481 aaaaggtcta aaacgtaaca cattaatttg cgcctttttg aatttggctg ccgaaaccat
    33541 tttcattgca ctggtaatct tctttgtgga agctaccgat gctattctgg ttcgtatttc
    33601 tttcaaattg gccatcccag aaatttattt aaattccgtg taatttttgg caacctcttc
    33661 ggctaggttt gttaatgcat cggtaatttg gtcgttaaga tcgccttttg ccatttgttc
    33721 cagttccgat ttaagtaaaa gctggcaacg ttccaggaaa tccttttcaa aattgcgaac
    33781 ttttcctgta gggatatttt taagcaaacc ttttgttccg cagaaaataa ttgcaacctg
    33841 gagttcaacc ggtaaaggct ggtattgtcc ctgtttaagt atctcaacgt ttttagcccc
    33901 tttgtccaaa acggcaagtg tggcagcatc taaatcggcg ccaaacttgg caaaagcctc
    33961 aagttcgcga tactgagcct gatccagctt tagggtgccg gcaaccttac gcatcgattt
    34021 tatctgtgcc tttccaccaa ctcgcgaaac cgagatgcct acattgatgg caggcctaat
    34081 accgttgttg aacaggttcg attcaaggaa tatttgacca tcggtaatag atatcacgtt
    34141 tgtagggatg taagccgaca catcacctgc ctgggtttca ataattggca atgcggttag
    34201 cgaacctcca cccttaatca aaggtttaag tggcgatggc aaatcgttca tcatggaggc
    34261 tatatcatcg gattcaataa tttttgctgc acgttccagt aagcgcgaat gaaggtagaa
    34321 tacgtcgcca gggtaagcct cacgccctgg agggcggcgt aatagaagcg acacctcacg
    34381 atacgaaacc gcttgctttg acaaatcatc gtaaacaacg agagcgtgcc gacctgtatc
    34441 cctaaaatat tcacctatgg tagctccggc aaatggtgca taaaactgca tggcagcagg
    34501 atcggatgcc gtggcaacaa caatggtggt gtaatccatt gctccgctat cctcaagcgt
    34561 tttagcaatg cttgctaccg ttgacccttt ttgtcctata gcaacataaa tgcagtaaac
    34621 aggatcgccc tttaagtaat tgtttctttg gttgataata gtatcaatgg caattgccgt
    34681 tttccctgtc tggcggtcgc caataataag ttctcgctgt cctcttccga tgggaatcat
    34741 tgagtcaatt gctttgatac cggtttgaag cggttctgac acaggttgtc ggtaaataac
    34801 acctggagct ttgcgctcaa gtggcaaaac aatggttttt ccggttatag ggcctttccc
    34861 atcaattggt tcaccaagcg tattaacaac tcgaccaaga agtccatcgc caacaggaat
    34921 tgaggcaatc tcgccaagct gtttaaccct gtcgccctcc tttaccagtt cggtggggcc
    34981 caaaagaact ataccaacat tatcctcttc aaggtttagg gcaaccccct ttacacctga
    35041 ttcaaactca accagttcgt ttgactttac attgtttagc ccaaaaacac gagctatacc
    35101 atcgctaacc tgtaaaacaa atccagtctc ctcaaatctt gctttggttt caatacctgc
    35161 aagttcatcc ttaatgagtt gagttatttc ggaaggtttt attcctgcca tagcttacct
    35221 gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaggattt caagcaaatg
    35341 acgtataatg ggataaatgc tttcaagttc attgttttcc tgacagaact caaaaagccc
    35401 ttttgcgtaa cgcgatgcta ttgatccgga actcattttt agtttttagg tatttcgtcg
    35461 agtaaacgtt caatatactc ctgttgtttt tctttatcct taagctgggc gcgcaaaact
    35521 ttttcggtta ggcttatggt taactcagca acagtggcat aaagctcctt tttggcggcc
    35581 tctttttcca aaagaatttg ctcacgggct ttgtcgagca tcttttgggt ttcaatgcga
    35641 gcctgctctt tggcttccgc cagtagcttt tccctcaaag ctttagtttc ggcaatttcg
    35701 cgttcgcgtt cttgcctagc cttttcggcc ataagcttgc tttcctcctg caatttttct
    35761 atctcggcac gagcttgctc ggcaaggcgc attgaacgag ctatgctttc ctccctgctt
    35821 tttaaggcct gcagtatggg tttccatgca aactttttaa gaatgaaaag cagcacccca
    35881 aacgatagca aagcccaaaa gagtaggccg tagtcgggtg taacaagacc cataggttaa
    35941 agttttatac aaataacgcc acaataagta taataagggc aaaaaatgca accccttcaa
    36001 taagcgcagc cgaaacaatc atattggttc gaatatcgcc tgaggcttca ggctgacggg
    36061 ctatagactt tagagccgaa gagcctatgc gaccaatgcc cagtcctgcg gctaaaacaa
    36121 ttattgctgc acccaatgca atgccaaatt ttgcaattga tgcgccatcg attgactgaa
    36181 gtaaaattag taatgtattc atagttttat atttaagttg ttagtgatgt tcttctgtag
    36241 ccattccaat atatagggct gtaagcaaag taaaaacata tgcctggata agggcaacca
    36301 gtaattccag caacgacatg aatataagga aaaccagaga aatgggtgca acggcaaatc
    36361 ctaaccaagc tatcattgaa gaaaaaatga aaatcaagct aagcagaacc gtagctacca
    36421 tgtgcccagc aagaatattg gcaaacaaac ggaccattaa aacaaatggc ttgatgaata
    36481 ctccaataaa ctctaccagg ggcatgattg gtatgggcaa cttcaaaaat gttggtatac
    36541 ccggtgggtt aactatatgt ttccagtaat ctttgttggc atgccggtta attgtaaaaa
    36601 aagtaaaaag cgcaagagcc atggttacgg atatgttacc agtaacatta gccccccctg
    36661 gaaacaatgg aataagaccc aaaagatttg agaaccatat aaaaaagaaa agagttaaca
    36721 aaaagggcat gaaatggtta tagcgtttct ctcctataga agggattgct atttcgtccc
    36781 gaataaatat tataaatggc tcaaaaacat tttgtaaacc tttgggtggt gaatcgggat
    36841 ctttcttata ccttcgggct atgccaatga aaattaaagc caacaaaatt gatgtgaata
    36901 gtattgaaaa ggcatttttg gtaatggaaa aattataggg taatggattg gtttcgtcaa
    36961 cttctcccct attgtttaac tccacaattt tacctttaag ttttccttct gtctcaatcc
    37021 tgaaaccttt ataaacgcca tgatcatgat ggaacttgtt ggaaagaaat acatgcaagc
    37081 ctgttttttt gctgtaaagt attactggaa gcggaatgct aatatgatgg tgatttacgg
    37141 taagtatatg ccactcatac gaatcgctta cgtggtcaaa tatgaattcg gcagtattaa
    37201 accccttaga gtcttttttg tgtacttcgt cagcttgcgc gcaatttatg cttgcggcaa
    37261 tgagtaacaa taaaattaaa accgataggt taattctttt cttcacttgt attcctatcc
    37321 tttacaataa agtatgtgaa atgcaaaagt aaaaccagat acgatatcaa atatacgata
    37381 accaacagaa ttttgtgact aaaaccggct aaaaaaatta ctaagacagc tacgaggttt
    37441 actaaaacat tgattgactt aatgataaaa tacttactta catacttaat tcttggtaaa
    37501 actgattttg atgagattaa tgcgaaggct ataaaactca aagaagcagg aactaaaggt
    37561 attatcctta ctaatgtttc actggtaacg aaccatagaa ccaatagtaa ggataaaact
    37621 ataactaaaa gagtaatcgc aaatctttgt ttaacactca tttgatttac tcttgacctg
    37681 gaataacttc tttgctttcc attcctagca tattgcagta acctgtaaac ttgcaaccgg
    37741 gttcaatagc aagtttacct acagtaatat cgccatttat ccgagccgat gatttgagcg
    37801 ataatagctc atcgacagtg attttaccct caacaacacc tgaaatatct gagtttttgc
    37861 aaaccaactc accttttatt ttaccggttt caccaacaac aactttgcct ttaacattga
    37921 ggttaccaac cagaatgcca tcaatacgca aatcgccact acttttaata tcgccattga
    37981 tttcagtgcc aaatccaacc aggttgatgt tgttagcatt aaattcgggt tcatttattt
    38041 ttgccatagg taaaaagttt aatgtttaga attacaaata taaaaattcc gtttaaagtt
    38101 tttccttatc gctttcgtca aaaatttcac tttcccaata ttctgaagtt tcttcagcat
    38161 catcagcagt ttcgctcatc cagctccagg tttgtggagg aggtccttgc cttcggtaaa
    38221 caaaagcgag tattgcacct gttattcctc caaataaatg gtattcccac gaaatttgag
    38281 ggaatattgg tagtacgccc caaaccaagc ctccataaag aaaaacaacc aaaagcgata
    38341 ttgccatcag gtttatgttc ttgcgaaaaa ctccactaaa aaagataaac gatgcaatag
    38401 catatataat accgctggaa ccaatatggt aactagtgcg agcagcaatc caaaccatca
    38461 ttccgcttaa caaccagctt agtgaaagta ttttatacca taaatctctg taaaaataaa
    38521 ctaaacctgc aattaaaata aatattgtta ttgtgttatt tattagatga ttaaagtcag
    38581 agtgaattaa aggagcggt
//
LOCUS       DAOOWJ010000009        37700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1132_length_37700_cov_7.355505, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000009 DAOOWJ010000000
VERSION     DAOOWJ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 37700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37700
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_37700_cov_7.355505"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /gene="mnmE"
                     /locus_tag="PLW22_01880"
     CDS             <1..1256
                     /gene="mnmE"
                     /locus_tag="PLW22_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOU73481.1"
                     /translation="QKAYTIHYGSIVDGDREVDQVLVSLFRAPHSYTGEDVVEISCHG
                     STVIQQQLLELLVRHGARLAQPGEFTLRAFMNGKIDLSQAEAIADLIASTSETARRVA
                     LNQMRGGISNKLAVLRNELLRFTALVELELDFSEEDVEFANRSELEGLINSIISEIDK
                     LSASFSQGNAIKMGIPVTIAGKPNVGKSTLLNRLLNEEKAIVSEIAGTTRDAIEDTIH
                     LGGINFRFTDTAGLRQTTDTIESIGIERAFAKIEKARIVLYLSDAQANLDEIISEVEA
                     FATKLTSSQNLIVLVNKEDKSTQAHVEKVLSELAGKGFTALALSAKAGHNIEQLQQLL
                     ISMGKADMPENEDVVVTSIRHYNALQSARASLTQVLNGLQANLSGDLLAIDIHQAIDY
                     LGEITGEISNDEVLGFIFSKFCIGK"
     gene            complement(1258..2121)
                     /locus_tag="PLW22_01885"
     CDS             complement(1258..2121)
                     /locus_tag="PLW22_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU73482.1"
                     /translation="MINFRLSNISIAVLFIALLFTVGCNSNKNDSFRVMSFNIRFDNP
                     ADGLNAWPYRKEHVAQMVKFYNIDILNVQEVLHHQLLDITGMLNGYGFIGVGRDDGDT
                     LGEYSAIIYSTQRFELLFSKTFWLSETPDTPSLGWDAACKRVCTWAIFKDRISGKTFA
                     LFNTHFDHMGKEAHKNSARLLVNRITAIADGLPVVITGDFNMIPTDSSLVPVTSNFAD
                     TYDLSPIGHYGPVGTWNGFDYSSPLTDRIDYCFVDSNKIDILRHAHIDDAFRQRFPSD
                     HLPVFVEMRVS"
     gene            2242..3639
                     /locus_tag="PLW22_01890"
     CDS             2242..3639
                     /locus_tag="PLW22_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU73483.1"
                     /translation="MISFILNDKPVNTSCNPGMSLLDFIRYESNLPGTKIGCREGDCG
                     ACTVLIGELTNDTVEYRSVTSCLYPLINAHGKHVVTIEGINLSNTLSPVQQAMADNAA
                     TQCGFCTPGFVMSFTAFCMNHETPTTERAIEAVAGNICRCTGYKSIERAAADIARVMQ
                     EKKISNPVGWLVGKGYLPNYFLEVPGKLKQIKAFDDESSSTSQLIAGGTDLMVQRPES
                     IYEQKVKSTLDGHLPKEITLNDCTCTVGGATRVSDLVASAELMERFPHLKEYLMLVSS
                     EPIRNMATIAGNIVNASPIGDLSVMLLALNAQITSVSDGKTRQQPLNQLFKGYKQLNL
                     NAGEVIQYITFELPKSTTQFSFEKVSKRKYLDIASVNSAMLIEVENGKVKKCRISAGG
                     VAPIPLYLHQTSNYLSGKEINSQSVTQAITIMHDEISPISDIRGTAEYKKLLLKQLFL
                     AHLQRFLGKQFDQIF"
     gene            3651..5906
                     /locus_tag="PLW22_01895"
     CDS             3651..5906
                     /locus_tag="PLW22_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU73484.1"
                     /translation="MKNIDTIGHVTGKSVYLDDIPTISGTLHAAVVGSAIAHGKILKV
                     DTTEALRLDGVVKVITCTDIPGENQIGGIVEDETLLAEGEVHYQGQPIAVVIAKTERD
                     AHKAAKMVRIDYQELEPVTDPRIAKEKGLLLIPPRTFSMGNPNEVWSQCKYIVEDSIE
                     LGGQEHLYIETQGAYAYPVEGGYIKVHSSTQGPTAVQKTVARVLGLPMNLIEVDVVRL
                     GGGFGGKEDQASGFAAMAALAAHITGKPVKLVLPRHDDMRMTGKRHPYSGDFRIGLTA
                     DLKILAFETTLYQNGGAAADLSPAIMERTLFHSTGSYYIPNTRVTVYSCKTNLPPNTA
                     FRGFGGPQGMFVIESAIAKAAETIGVSAIEIQQANLLKENDEFQYGQKAENVNARKCF
                     ALASERFDLENLRKSIDNYNQNNKYSKRGLAVMPLCFGISFTNTAMNQARALVHIYQD
                     GSVSVSTGAIEMGQGVNTKMAQVASILLGIDISRVKIHSTNTTRVANTSPTAASSGAD
                     LNGNALRIAIAALKKRLLKVAAELLECSDDELNIENETVIRNGAPTALKWNELVKKAH
                     LKRVALTENGHYATPVIYFDKTKEKGHPFAYHVYGTGVVTVTLDCIRGTYTIDDVYIV
                     HDFGNSINPLIDLGQVEGAVVQGIGWLTLEELAFDSKGRLLSNSLSTYKVPDIHFAPK
                     SIQCIPLPTNGPELAVLRSKAIGEPPFMYGIAAYFAIHNAIKAFNPHYSIKLTAPLTP
                     ERVLMNLYQGR"
     gene            6211..6765
                     /locus_tag="PLW22_01900"
     CDS             6211..6765
                     /locus_tag="PLW22_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73485.1"
                     /translation="MEVLFDACVFFLLFYPTFLRAQAAVETYKQAIRLKPNYVEAYYK
                     LGMDYVNLGRYTEAVEAYKQAIRLKPDYADAHFNLGLTYGQLGRYTEAVEAYKQAIRI
                     EPDDAEAHNNLGTAYDKLGRYTEAVEAFKQAIRLKPDLAMVHYNLGVAYLHVGDKSSA
                     FEVYKILKELDKDLSNKLFNLIYK"
     gene            7146..7682
                     /locus_tag="PLW22_01905"
     CDS             7146..7682
                     /locus_tag="PLW22_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73486.1"
                     /translation="MNTFAIEIWYDEGSICTFYTVRWVTDDDNAPSETDRFFDTYAVP
                     EHPLNEKAMQLFRLITESIGNRYGATNDFFDRIVNKAQELPPKQKQWVEEIKDLGINF
                     PLRLFCYRVTENIVILFNGGIKESQASQESKNLRMKFYEAQTFVKKIEEALQSQMIKI
                     SDNGRYLQNFDGTTDIIL"
     gene            7687..8100
                     /locus_tag="PLW22_01910"
     CDS             7687..8100
                     /locus_tag="PLW22_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU73487.1"
                     /translation="MRSKTVDRLLKSTPKDVEIFVDWYADLVVRINQLLREKNISKKE
                     LSVKMNKKPSEISKWLNGEHNFTLRSLAKLSAELGVPLIEVTKKKVQTEFVEDGFVCR
                     VHTFVSYTKLNTKTTENVWQCAEETEPYNELSNAG"
     gene            8090..8542
                     /locus_tag="PLW22_01915"
     CDS             8090..8542
                     /locus_tag="PLW22_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73488.1"
                     /translation="MQDKNVFDPEKIALIDFKMIKGQVNTPENFDINKVVGYHLDNSL
                     QLSFNLDDKLAKADFTVNVKTDSKGENESEATGNFHLIFIYRIENLEELTILKKNKRL
                     NLNPGLANALSSVTYSTSRGILITRLQGTALQNFVLPIINPNKLLHNK"
     gene            complement(8847..>9090)
                     /locus_tag="PLW22_01920"
     CDS             complement(8847..>9090)
                     /locus_tag="PLW22_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73489.1"
                     /translation="QADYVYPVYEGRGGHPILISSRIAKAARAETNPDINLKEYLNRF
                     SKACIPVPNDQILININTPEEYARLIADGKLPGNGI"
     assembly_gap    9091..9190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9191..9376
                     /locus_tag="PLW22_01925"
     CDS             <9191..9376
                     /locus_tag="PLW22_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73490.1"
                     /translation="SKALGCPIRPEEPAARIMAEKFSFIRLYSNILCQIAIKCYTKLI
                     CLAIFYRFVGNIRIYER"
     gene            9366..>10528
                     /locus_tag="PLW22_01930"
     CDS             9366..>10528
                     /locus_tag="PLW22_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73491.1"
                     /translation="MSDKFSPIPAKQLLEIILKELDTHGTVFGYPSELFFNPNQGKLP
                     TTIFGQTIDTPIGVAAGPHTQLAHNIVIAWLMGARYLELKTIQTLDELEIAKPCIDMQ
                     DEGYNCEWSQELRVEESFMEYLKAWVIIHILNHKLQLGSKPNMVFNMSVGYNLEGIKK
                     PNVQWFLNKMGDATGELNQMVQELKAIYPAIGNIDIPAKLSDNITLSTMHGCPANEIE
                     DIARYLLTERRLHTLVKLNPTLLGSSELREILNNKLRFRTNVPDIAFEHDLKYPDALK
                     LIEALSAEAKTLNLTFGVKLTNTLESVNNKHVFPGEVDMMYMSGRALHPISINLARKL
                     QNDFKGQLLLSFSAGVDAFNVSKVMGCGFRTVTVSSDLLKPGGYGRLKQYIDNL"
     assembly_gap    10529..10628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10629..12422
                     /locus_tag="PLW22_01935"
     CDS             <10629..12422
                     /locus_tag="PLW22_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU73492.1"
                     /translation="PFPNTTGMVCDHLCQNKCTRINYDNPLQIRDVKRYVAENADIKL
                     SPAQPNGKRVAIIGAGPSGLSCAFYLAINGFKVDVFESKTKAGGMVRYAIPGFRLTDQ
                     AIDRDIERITQLGVNIHYSHPVTRSEFSDLKNKYDYVYISAGAQKSAILDIEGIDADG
                     VIDPLTFLMGVKSGNIKQIGKRIAIIGGGNTAMDAARTAHRLVGDNGKVTIVYRRTVN
                     EMPADEGEIKAVIAEGVELVELAAPEKIITQNGKVKALKCSLMKLDGYDSKGRPKPVK
                     VEGSEFEVECDVVIPAIGQALDIDFVPNELLKADTRNYKTLMGNVYIGGDALRGASTA
                     INAIGDGRKAAQEIISQCGFTGISTGVPEKKHNYRDLMLLRSTRKYGYVHKELTAEKS
                     KTFELVTETPDETTIKEESARCLHCDEVCSICTTVCPNLANFAYTIEPVRYQLDKVLV
                     GSDGTQEVQPDHFFEVKQTVQILNIANFCNECGNCSTFCPTEGAPYQDKPKFHLTTKS
                     FKESEDGYMLSVLKDRNVLIFKQKGGIKTLSENRDEYIYETDHVYARFSKNGFKLVEA
                     KALTPCVRQIQFQHAVEMSVILQGAKRLMGV"
     gene            12586..13668
                     /locus_tag="PLW22_01940"
     CDS             12586..13668
                     /locus_tag="PLW22_01940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU73493.1"
                     /translation="MRILQLVTRFDFGGAENHVRELCNELAASNHQVILLSRKGRQNE
                     LLDKRVKFIQLPSFVSYLILTKVVIIVYLLLRHKIDVIHAHQRLPIISACIGGFILRV
                     PVVATVHGRVRHDLRSIVAKKLTSKIIFVRNQAISNLDRFRSIQNKSVYIPNGIPIPK
                     KLPLIETNTIGYICRIDKRHFRVIKLLILACEKLKLSQPTIKLLLIGDGNNVEELKEL
                     INKTNLKFESTAIEYLGFVRNIDLLNSFPELILGVGRVAIEGLIRGATVISIKNNKMG
                     RIITPSNYDDYAFTNFIKIDGCVPTEESIYNELKLYFERRNEYRKNTSILAARIENDF
                     GISKTTNRIIEVYSSTLKSRGLPNSK"
     gene            complement(13720..14181)
                     /locus_tag="PLW22_01945"
     CDS             complement(13720..14181)
                     /locus_tag="PLW22_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOU73494.1"
                     /translation="MRNIDEIDRKLLNILQDNSRVTIRELSERLHLSTTPIHERIKKL
                     EKNGYIKQYITLLDPKALGKKLTVFISVSLSSHTKEVIDAFELEVKKLPEVMECYYVS
                     GNWDFLLKAQCNDMEDYHNFVIHKFSVIKNITQFYSSFVMSESKQTYKYEL"
     gene            14371..15849
                     /locus_tag="PLW22_01950"
     CDS             14371..15849
                     /locus_tag="PLW22_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU73495.1"
                     /translation="MIEIIDRITNPKALENAVKRFREKGIILPTFHQQQNPEQIPDKI
                     KVKLKDVGLWEINPVNLFRITWKNEPKEKGGLFGKVNYLELPKELTGVNARIILLLGK
                     WFPTGAHKVGAAYGCLAPRIITGGFDPTTQKAVWPSTGNYCRGGAFDSKLMGTESVAI
                     LPEEMSRERFTWLRDYVGSEVIATPGCESNVKEIYDKCWEIRRTKPDYVIFNQFDEFG
                     NAAWHYNITGKAIEEVFNELGGKSNLAAYVSATGSAGTIAAGDYLRTIAPHIKVVASE
                     AKQCPTILMNGFGGHRIEGIGDKHVPWIHNVKNTDVITAIDDEDCMRIFRLFNEPEGV
                     KYLVESGVPVEIANNLKLLGISGISNMLSAIKTAKFFEMTSEDVIFTIATDSAEMYGS
                     RLEELREERGSYSQIQAIKDFEKCILGATTDWMRELTYHDRKAIHNLKYFTWVEQQGK
                     EIDDLNRLWNDRELWPKLFSQVKRWDELIDEFNDRVGLLKSL"
     gene            15861..17096
                     /gene="thrC"
                     /locus_tag="PLW22_01955"
     CDS             15861..17096
                     /gene="thrC"
                     /locus_tag="PLW22_01955"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU73496.1"
                     /translation="MNGKFILTCSGCGKTFAPSPELYLCPDCSRNNDTVNPPKGVLKA
                     LYNYEILSKHKEEVITGNFWDLIPLLKKESLPKLRVGETPLYRFDRIDGETLTFELYL
                     KDDSQNPTFSFKDRASAVVSAYAKENGISTIVAASTGNAGSSLAGICASQEQKAIIMV
                     PKSAPHAKLTQIVMYGATIVPVNGTYDMAFDLSMEATKEFGWYNRNTAFNPLTIEGKK
                     TVSFELFKQLSRAIPDYIFVPVGDGVIISGVYKGYEDLLKIGLIDRMPTVVAVQAAGS
                     SNLIENIGKPDFTIKPSNTLADSISVDIPRNFFMAQDYLLQYKGEWLSVTDDDIVNAS
                     KILARNTGIFAEPAASTAFAGMLCYQKNQKLLTKQRVVVLLTGSGLKDLKAVSSVINI
                     PNAIDPTIDSLRKNEWIKF"
     gene            17204..19762
                     /gene="xdh"
                     /locus_tag="PLW22_01960"
     CDS             17204..19762
                     /gene="xdh"
                     /locus_tag="PLW22_01960"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HOU73497.1"
                     /translation="MIRFNLNGKTVEYNGDINRSLLEYLRNDLHLTAAKDGCSGQGVC
                     GACSVEVNGSIKMACRIKMSDLSEAIIYTLEGLPNNFREILAHHFATKGAVQCGFCSP
                     GMIMRARGLWLSNPKATRSDIIRAIAPNLCRCTGYVKIIDAIQHAFAELNGQALPKNN
                     TGARIGERYPKYQAKETALGQRPFVDDMFVDGMLYGALKFSDHPRAKVLKIDTSKALS
                     VQGVVKVITATDIPGERYIGLIFQDWPVMVALGEVTRYIGDVIAGVVATSEDIARHAA
                     SLIEVEYEVLEPVTDIHQAIKPDSPQVHPDRSNVLETCTIRFGEVDKAFAEAAYVSSD
                     IYETQRIEHAFLETEAALALPNGDGIKVFSQGQGVYVDRKQVAKILNLPESKVNVVQV
                     QNGGGFGGKEDLTVQGHAALMSYITQMPVKVHLTRDESIRMHPKRHPVWMKMSLACNS
                     EGKFTALKLEAIGDTGAYASVGTKVMERVVGHATGGYTVPAVDISAKTVYTNNIPCGA
                     MRGFGVPQVTFAIESCIDDLCRQGGFDRWQIRYDNALEESAKTATGQELHGVGLKKTL
                     IAVKEQFKSAKYAGIACGLKNTGVGNGMVDDSEVIIKVISPTRVEIHHGWTEMGQGVH
                     TMAIQTLHHETGIEPSCIEVIVDTDAEIPTGMTTSSRATALVANALINAARPLREDLK
                     TAKLEKLVGRVYRGKFVCDWTCKPGAKTDKPVIHFAYGYATQVAILNEQGKLSKIIAA
                     HDAGKIMNQMLFEGQIEGAVHMGMGYALTEDLPMENGFPVSFKFKDIGIIRANQMPEI
                     EVIGIEEPDPIGPFGAKGIGEIGMVPTAAAIANALWTFDGIKRTRLPMMRNKSE"
     gene            19788..21053
                     /locus_tag="PLW22_01965"
     CDS             19788..21053
                     /locus_tag="PLW22_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU73498.1"
                     /translation="MILLSDATYIDYQTLEIKNCDILVTNGNIEFHPPKTYRGNATET
                     INCSGKLVTHSFIVGHHHAYSALARGMGAPKKSPENFYEILKYIWWTLDKCLTTEMVE
                     YSGLVTAMACAKAGATFVIDHHASPYAVKGSLETLAKAFERVGLSHLLCYEISDRDGD
                     EIAQQGLEETDSYLKNYQGLVGLHASFTLSDKTLQAAADLTQKYNSGVHIHVAEDKYD
                     QQHCHETYGKTVVERLSDFNFLNSSKSILVHCLHLSEKERNLIGQSPCWVAQNTDSNL
                     NNNVGYFSSKNLGENIMLGTDGMHSDMLQSMKSAYFVGQNFDPMSPAQAYKRLRNAHH
                     YIAQNGFSGDGPDNLVVLDYDSPTPVNAENIPGHFIYGINQSHIEHVISNGKLIVKSR
                     AITTVDQEIILKESQKLALELWKRMRGEG"
     assembly_gap    21288..21387
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21440..22063
                     /locus_tag="PLW22_01970"
     CDS             21440..22063
                     /locus_tag="PLW22_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73499.1"
                     /translation="MNQQVLEWIGYSASVIIALSMTMSSIVKFRWINLTGALMFSIYG
                     FLIGAWPVGILNGFIVLTDAYYLFQIYSKKEVFEILEVRPENRYLMRFLDFHQKDIHR
                     FFPGFVYTPTENTVSFLILRNMAVAGVFIAQRNETKALEVELDYVLPEYRDFKNGKFV
                     YYWLSQKFTESGYTRVWAKACNKSHKKYLQHLGFTESGGNHFLKILE"
     gene            22073..23503
                     /gene="hydA"
                     /locus_tag="PLW22_01975"
     CDS             22073..23503
                     /gene="hydA"
                     /locus_tag="PLW22_01975"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOU73500.1"
                     /translation="MRILITKGRIVTSVGEQIADLLVVDGKIAAIGNQLEANAETTIN
                     AQGMLVLPGGIDPHVHLHLPTPAGFSSDDFHSGSIAALMGGTTTIIDFVTPSRGQSMV
                     EALKARLDEAACSLVDYTFHVSPIEWNKNTPKEIEECIEMGFPSFKVYMAYKSSVGID
                     DNALYHVMHTVAQNGGMVTAHCEMGDDIDTLRDFYASQGLTAPKYHMLSRPPRFEALA
                     VKRAIDLADQTQCPLYIVHVSAAESVKHIIQAQKSGQPLYAETCPQYLLLDEALYDSS
                     LPEAIKYVISPPLRQSIDRDTLWNAINNGNILTVGTDHCPFSLEQKMVGKDDFRKIPN
                     GAGGIEHRLALLYTFGALTGKLSLSKLVDVFATQPAKIFGLYPQKGELAIGADADIVI
                     WNPNVKGSISSSKHHSKSDISIYEGFETTGEVAFVIKNGIIAVDNGKLSENLPSGQLL
                     KRRSTKTTGHYYKRMDNSGSYNCGRV"
     gene            complement(23514..24056)
                     /locus_tag="PLW22_01980"
     CDS             complement(23514..24056)
                     /locus_tag="PLW22_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73501.1"
                     /translation="MKELIEEIFIDAWAINDIISCYQDKKADYPDLFQIVSGYDLSKP
                     TNKIPFKVYVELCSWIEKKLGQFNLIKIGRRIGESTYSLMLETNMVNTESKPLDVMKA
                     LVLLAQKGVKDPKRRGWEIVSHTDKSIQMRKTQLFNTHIQVGLLDTLVRKCKVYGVQV
                     NLVKEESKGAEFDEYLITWL"
     gene            complement(24312..25499)
                     /gene="ygeW"
                     /locus_tag="PLW22_01985"
     CDS             complement(24312..25499)
                     /gene="ygeW"
                     /locus_tag="PLW22_01985"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOU73502.1"
                     /translation="MDLIKKIAELNHYKSDLSGKDFLLTWEKSDDDLKVVLQVASILK
                     EMRKENISPRIFDSGLAISNFRDKSTRTRFSFASAANLLGLAVQELDEEKSQISHGET
                     VRETANMISFLSDFIGIRDDMFLGEGNKYMRQVGEALDEGFTKGVLPVRPGIVNLQCD
                     MDHPTQSMADLLHLANEYGSLDALRGKKIVMSWAYSPSYGKPLSVPQGIIALMTRFGM
                     NVELAYPEGYDLIPEIVEIARKNATQSGGSFKVSHSMTDAMRDADIVYPKSWAPFHIM
                     QQRTQLLMNGDKNGLKELEQVCLANNAKYKDWEYNEQVRKVTRNSDALYMHCLPADIS
                     GESCIQGEVSKETFERYRIKTYQEAGFKPYIIAAMMLTNRFQNPADILMKLIERNTPR
                     VIK"
     gene            complement(25779..26966)
                     /locus_tag="PLW22_01990"
     CDS             complement(25779..26966)
                     /locus_tag="PLW22_01990"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOU73503.1"
                     /translation="MSDVYSKIKELSEKYADYTARNLSRLVQIKSLSAQERDVQLELK
                     RQMEEAGFDEVFIDGLGNVIGRIGNGKRILAIDGHMDTVDIGNKDNWEFDPLGGEIRD
                     GYVHGRGTVDQEGGPAAFVTAGKILKELGFNRDLTIYFVGSVMEEDCDGLCWKYIVEE
                     DKIRPDFVISTEPTNLNIYRGHRGRMEMQVHFYGVSAHGSAPERGKNAIYMASKVALE
                     VEKLNERLKFDEFLGKGSITVTEFTSSSPSLCAVSDYARIHLDRRLTWGETKESAVAE
                     IEELIKGMNAKVEVLNYSEKAYTGLEYGMEKYYPTWKMPEDSEIVQKGVSTYQKLFGN
                     APKVDKWTFSTNGIMTCGTYKIPTIGFGPGNEVLAHAPNEKVPISDLVAATAFYAAFA
                     YEI"
     gene            27186..28139
                     /locus_tag="PLW22_01995"
     CDS             27186..28139
                     /locus_tag="PLW22_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOU73504.1"
                     /translation="MIWQFINSKIEQNIDVYLITVAETQGSSPGKVGFKMAVAADGSL
                     YGSIGGGVMEFNLVEMARKNLAEGYKQNFLKRQVHSHDAAEDKSGMICSGEQTQIFSL
                     FSKADSEVLAKVIELESENRPGKIVLSTSGAEVYEGESHIGIKGIEFQKSDSSFRYSE
                     PLGVKETVYIFGAGHISVPLSQILGLLDFRVELFDNRQGLSTFEGNVWAHKKQIVDYK
                     QVSHLLPGNDRSYVVIMTFGHKFDEIVLKQLIGKPLKYIGMIGSKSKVKTIFESLIAE
                     GYGKENVERVCSPIGLPIGGQTPAEIAVSIAAQIVEWRNKR"
     gene            complement(28142..28819)
                     /locus_tag="PLW22_02000"
     CDS             complement(28142..28819)
                     /locus_tag="PLW22_02000"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOU73505.1"
                     /translation="MKKIILALSVSLVSAIGYAQQTAVYSLKEFDKLRVWGEFKVFLV
                     KGDSLNAKVETQSIPASDIALDVNGNTLEVKLKGKLYDRVSATVYITYRELREISVSA
                     AAAVSLQDTLKATNISININTSSEFDGAVLAETADLTVGQGSTLRIRGKVSSYEAKVN
                     TGGVLTAIELIAPKTYVKVNTGASAKVYASKVLEANVSTGGSLTYTGSPEQKKIKTLV
                     GATIIEQ"
     gene            29020..29406
                     /gene="msrB"
                     /locus_tag="PLW22_02005"
     CDS             29020..29406
                     /gene="msrB"
                     /locus_tag="PLW22_02005"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOU73506.1"
                     /translation="MKSDDLRNRLTDIQFYVTQQKGTERPFTGEYWNHFEPGLYVCVC
                     CGAELFQSDTKFHSSCGWPSFFDSKFVENIKFQKDLSHGMVRTEVLCKNCGAHLGHVF
                     NDGPEPTGIRYCINSASLKFIPNKKE"
     gene            29410..30012
                     /locus_tag="PLW22_02010"
     CDS             29410..30012
                     /locus_tag="PLW22_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOU73507.1"
                     /translation="MGSTTKYMAMFPLPVFLLPGEEIPLRIFEPRYKQLINECNETGD
                     TFGIPYVSNDNIKSYGSEVELVNIVAKNSLGEMVILIKAIANFHLLNLKEKLPDKLYG
                     GGIIEYIDDRFTTTNPELVVLVKKLNLNLDPIMGSLITGDSVNLMNVAKAIQLDSDEK
                     FAFYSLRNEKQMVNYLIKRLRFIEHIQLQEKLLENNFSLN"
     gene            complement(30009..31310)
                     /locus_tag="PLW22_02015"
     CDS             complement(30009..31310)
                     /locus_tag="PLW22_02015"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU73508.1"
                     /translation="MIILLSAILIGFLVFSFGIYLKKNNRTLALPIAESSKYAKVDLL
                     SVNNALTNSKEYSSINHQFEQFVNKWDLVGATFCIAHKGKIIYARGFGMANKEEGLAM
                     QPYNTMRVASVSKLITTTAVMKLVEEGKLRLNDRVFGADGILNDEKFLNYKDKRVEEI
                     TVHNLLNHSGGWTPRWGDHLFIKDVIARELHKDLPLDVDDYIVFALSKRLHFQPGSHS
                     SYSNIGFVILQRVIEKASGMDYESYVKNEIFKPLNIYDAHLANNWDSLRYDNEVRYYE
                     VPEAEKVISFDGSKQSVLKSRGGNDVRALGAAGGWVISPISLTRFVMAVDGRNDFPDI
                     LSKQSVDIMKKTYNGKFQPLGWRWVRDDGTLWRSGSMAGTSALVISRPDGYTLTFTSN
                     QSHWKGARFPYVVDRFFLRIFNKYIKSIPDHNLLNVDKGKY"
     gene            complement(31603..32643)
                     /locus_tag="PLW22_02020"
     CDS             complement(31603..32643)
                     /locus_tag="PLW22_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOU73509.1"
                     /translation="MFSRGFASDNNSGVHPRILEAINDVNHGHTIAYGDDPITQKAID
                     RFKQIFGNDIEVFFVFIGSAANVLGLKAVTQPHNAIICAETAHINVDECGAPERFTGC
                     KLLSVPTVNGKLTIDGIKKHMHGFGFQHHSQPKVISISQVSELGTVYTVDEIKTLADF
                     AHSHNMLLHMDGARLANAAVSLGRGFREFTRDSGVDVLSFGGTKNGMMYGEAIVFFNQ
                     QLAHDFMYTRKQGFQLASKMRYISAQFLAYLEDGQWDSTARHANAMAQLLAKKVSAIQ
                     GVTITQPVQANAVFAIVPPELIHPLQSEYFFYVWDEDRSEVRWMTSWDTTEQDIDRFC
                     QLLEEFMRTVKR"
     gene            complement(32692..35127)
                     /locus_tag="PLW22_02025"
     CDS             complement(32692..35127)
                     /locus_tag="PLW22_02025"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOU73510.1"
                     /translation="MKRWILLFSLSFLVGNIALGGRNKDSLRIAREIYAIRATEHIYI
                     NGRLDENVWGKSDFANNFTIYEPYNGQKASQQTEVSVLYDDFAIYIGARLFDTSPDSI
                     FRELGQRDNSDRLRSDAFSVYLSTYNDGVNYLQFTVSASGVQTDIKITSNQSDRNWNA
                     VWESAVGFDSLGWIVEMKIPYSALRFSKNVNQVWGINFSRLIKRKNEVSTWNFVDKAQ
                     AGFITQSGILKGISGVKPPLRLSFLPYISGYVDQYAGTKGLDSRVSGGMDLKLGLAQS
                     FTLDVTLIPDFGQVRSDDKVLNLTPFEVKYDEARQFFTEGTELFGKAGIFYSRRIGGT
                     PAFYDAAYDSLKTNEIVDKNPLETKMVNASKLSGRFANGLGFGFFNAMTENTYAEILD
                     TVSGKRRKLLTQGFTNYNITVLDQNLPNNSYVSLINTNVYRPVDSFIANVTATEFNLK
                     NKKQSFGISGVGALSQRFVDSVQHGFKTNLSISKISGNIQGYIWANIESEHYNPNDLG
                     YLRSPNELSSGVALAYKVFEPFWRLLNFEAQLEFSNSRLYTPKAFQSQEIEFQAKFTN
                     TNNFTGGFNASYNPRYGYDYYEPRVEGHKFLTPHVLWLQWWGSPDYRKTVALDHRFSF
                     WYADMYNQKGYMYSISPRIRFSDKLLTIYSWAYQSERNNIGYYDYDGSNIFFGSRKMV
                     TITNTLNIQLAFTAKSFLSIRARHYIKHFKYNEFFTLKNDGLLEQTNSITNPANRNYN
                     LFNIDMFYQWHFAPGSELVFAWKNQAEELLDNPQYLYFKNIEGILNSPQNNSFSIKIL
                     YYIDYQMIRKFKS"
     gene            complement(35256..36272)
                     /locus_tag="PLW22_02030"
     CDS             complement(35256..36272)
                     /locus_tag="PLW22_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU73511.1"
                     /translation="MKSSQVTIKDIARELGISPSTVSRALKDHPDISQETKRLVNELA
                     AKYNYKPNIIALSLRNQRSNVIGVIIPEIVHYFFSSVISGIEEVANEHGYSVMISQSA
                     EDYTREVAACDTFLNGIIDGILVSVSKETANYDHFHRFEEEGIPIVFFDRAVDEIQAD
                     KVIIDDFDGAYQATEHLIVQGRRKIVHFAGPQNRLIGQNRLNGYLKAMRDNGVVIDER
                     LIIPCDTFQSALVETQKLIDSGLKFDSIFTVNDFTAAGAMKVLLRNGYKIPTDIAVVG
                     FGDDQTSLMVEPTLTTVNQPGFEMGKKAMEQLIRRITQSKPEPPVTEILKTQLIVRES
                     SRVK"
     gene            36559..>37700
                     /locus_tag="PLW22_02035"
     CDS             36559..>37700
                     /locus_tag="PLW22_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU73512.1"
                     /translation="MNRLKQFLIILVALLLGNLSLMAQEISVTGKVTDAADGTPMIGV
                     AIVVKGTTTGTITNVDGEFSIKCKGNDVLVFSFVGYNTQEVAVGSRSVINVQMKQESK
                     EIDEVIVIGYGVQKKSDRTGSVVNVKAEELNRGSLTDPIQAMQGKVAGVNVSKKGGDP
                     NSGFAVQIRGAAGFASNTQPLYVIDGVPGADPTTISPDDIESFNVLKDASSGAIYGSR
                     ASNGVIIITTKKADKAKGATIDYNGYVSADQTSKRLEFLSAADIRKYVTDNNIPNFAD
                     NGANTNWQDEILKTGISQSHSIAVSNSTDNLSYRFSYNHTYNDGVVIQSNKQRDIARF
                     NLTQKALNDIITIDASIAGTFEKNNYVNYGGGMGSSNIFYQMYQRNP"
BASE COUNT        11258 a         7877 c         8019 g        10246 t
ORIGIN      
        1 agcagaaagc ctataccatt cactacggct ccattgttga tggtgaccgc gaggttgatc
       61 aggttctggt tagccttttc agggcgcctc atagctacac gggcgaggat gtagttgaaa
      121 tatcgtgcca tggttccact gttatccaac agcaactgct tgagttgctt gtgcgccatg
      181 gtgcacggct tgcacagccc ggtgagttta cattaagggc tttcatgaat ggtaaaattg
      241 atctttcgca agccgaagcc attgccgacc tgatagcatc taccagtgaa acagcccgtc
      301 gggtggcgct aaaccagatg cgtggcggta tttccaacaa gctagcagta ctgagaaatg
      361 agctgctcag gtttaccgca cttgttgagc tagagctcga ttttagcgag gaggatgtag
      421 agtttgctaa ccgtagtgaa cttgaagggt tgataaatag tataatttca gaaatcgaca
      481 agctctcggc atcgttcagt cagggtaacg ccattaagat gggtatacct gtaaccattg
      541 ccggaaaacc caacgtaggt aaatccaccc tgctgaaccg cttactaaac gaggagaagg
      601 ccattgtttc ggaaattgca ggaactacac gcgatgccat tgaggataca atccacctgg
      661 gcggaattaa cttccgcttt accgatactg cagggctaag gcaaaccacc gatacgattg
      721 agagcatcgg cattgaaagg gcttttgcaa aaattgaaaa agcacgcata gtgctttacc
      781 tatccgatgc acaagccaac cttgatgaaa ttattagcga ggttgaggcc tttgctacca
      841 agcttacctc atcgcaaaac ctaattgtgc tggtaaacaa ggaggataaa tcaacccagg
      901 ctcatgttga gaaggtacta tcggagcttg caggtaaagg gtttactgcg ctggcccttt
      961 cggcaaaagc cggacataat attgagcaat tacagcaact actaatatcc atgggtaaag
     1021 ccgatatgcc cgagaacgag gatgttgtag taaccagtat tagacactac aatgccttgc
     1081 aatcggctcg agccagtctg actcaggttt tgaatggcct acaggcaaac ctaagcggcg
     1141 atttgcttgc cattgatatt caccaggcca ttgattacct gggtgagatt accggagaaa
     1201 tatcgaacga tgaagtgctt ggcttcatct tctcaaagtt ttgtatagga aagtagtcta
     1261 gcttacacgc atttccacaa aaacgggcaa atgatccgat ggaaacctct gcctaaaggc
     1321 atcgtcgata tgggcatgcc ttaaaatgtc aattttattg ctatcaacaa agcaatagtc
     1381 aatcctatcg gtaagcgggc tgctataatc gaatccgttc caggtaccca ctgggccgta
     1441 gtgcccaatg ggtgataaat cgtatgtatc ggcaaaattt gatgtaacag gaactaatga
     1501 gctatcagta ggaatcatat tgaaatcgcc ggttatgact acgggcaaac catcggcaat
     1561 tgctgtaatc cggtttacca gtagtctggc tgaatttttg tgggcttcct ttcccatgtg
     1621 gtcaaagtgg gtgttgaaca gggcaaaggt ttttccgcta attctatcct taaagattgc
     1681 ccatgtgcaa acccttttac aggcagcatc ccagccaagc gaaggggtat cgggggtttc
     1741 cgataaccaa aacgttttac tgaatagcag ttcgaacctc tgggtactgt agatgattgc
     1801 cgagtattca cctagcgtat cgccatcatc gcgacccact ccaataaaac cataaccgtt
     1861 gagcatccca gttatatcaa gcagctgatg atgcagcact tcctgtacgt tcagaatatc
     1921 gatgttgtaa aacttaacca tttgagcaac atgttcctta cggtatggcc atgcattcag
     1981 cccatcggcg gggttatcga accttatgtt aaaactcatc accctaaagc tatcgttttt
     2041 gttcgaattg caaccaacag taaaaagtag tgcaataaac aaaacagcaa tactgatatt
     2101 gctaagccta aaatttatca tggtttccag aatttttcaa tttaattcct taaaaataca
     2161 tcaattttat tccattttta ctataaaact tgattttttt aatggttgat ttgtatattg
     2221 tgaagcatat taataacatc catgataagt tttatactta acgacaaacc tgtaaacacc
     2281 tcatgtaacc cgggaatgag tttactggat tttatccgtt acgagtcaaa cctacccggg
     2341 acaaaaattg ggtgcaggga gggcgattgc ggtgcctgca cagtacttat aggcgaacta
     2401 acaaacgata cagttgaata ccgaagcgtt acatcgtgtt tgtacccatt aattaacgct
     2461 cacggtaagc atgttgtaac catagagggt atcaacttaa gcaatacgct tagtccggtt
     2521 cagcaggcaa tggccgataa tgccgctacc cagtgcggat tttgcactcc cggatttgta
     2581 atgtcgttca ccgcattttg catgaaccac gaaactccta ctactgagcg tgccattgag
     2641 gcagtggccg gaaacatatg taggtgtacc ggatacaaat ccatagagcg ggctgcagcc
     2701 gatattgcaa gggtaatgca ggaaaagaag atatcaaacc ccgttggttg gcttgttggc
     2761 aaaggctatc ttcccaacta ctttctggaa gttccaggta aacttaagca aattaaggct
     2821 tttgatgatg aaagcagtag cacctcacag ctgatagccg gaggaaccga ccttatggtt
     2881 caacgccccg agagtatcta cgaacaaaaa gtaaaatcga ccttggatgg acatctcccc
     2941 aaggaaatta cattaaacga ttgcacctgc accgttggcg gtgcaacccg ggtgagcgac
     3001 ctggttgctt cagcagagct gatggagcgt ttccctcacc ttaaggagta cctgatgctg
     3061 gtttcgtcgg agcccatcag gaacatggct accattgcgg gaaacatagt gaacgcctcg
     3121 ccaattggtg atttaagtgt gatgctattg gcattaaatg ctcaaataac ctcggtttcc
     3181 gatggaaaaa cgcgccagca accgttaaac cagctcttta aaggctacaa gcaactgaac
     3241 ctgaatgctg gcgaggtaat tcagtacatc acctttgagc tgccaaagag cactactcaa
     3301 ttcagctttg aaaaggtaag caaacgaaaa taccttgata ttgcaagcgt aaacagtgca
     3361 atgcttatcg aagtcgagaa cggaaaggta aaaaagtgcc ggatatcggc aggtggggtt
     3421 gccccaatcc ctttatatct gcaccaaaca tcaaactatc tttctggtaa ggaaatcaat
     3481 tcacaatctg taacccaagc aataacaatt atgcacgatg agatatcgcc cattagcgat
     3541 atccgtggaa ccgcagagta taagaagttg cttctaaaac agcttttcct ggcacacctg
     3601 caaaggtttt tgggaaaaca gtttgaccag attttttaag gctaacagtg atgaaaaata
     3661 tcgataccat aggtcatgta acgggaaaat cggtttacct cgacgatatc ccaaccattt
     3721 cgggtacatt gcatgcagca gttgtaggct cagccattgc ccacggtaaa attttaaagg
     3781 ttgataccac ggaggcacta aggctggatg gcgtggtaaa agtcatcacc tgcaccgata
     3841 ttccgggcga aaaccagata ggaggaatag ttgaggacga aaccctgctt gccgagggcg
     3901 aagtccacta ccagggtcaa cccatagctg tggttattgc aaaaaccgag cgcgacgccc
     3961 acaaagcagc caaaatggtt cggattgatt accaggaact ggaaccggta accgacccac
     4021 gcattgcaaa ggaaaagggg ttactgctca taccaccccg aaccttcagc atgggtaacc
     4081 ccaatgaggt atggagccaa tgtaaataca ttgtggagga tagcattgag ttgggcggac
     4141 aggaacacct gtacattgaa acccaagggg cttacgccta tcccgttgag ggtggctata
     4201 ttaaggttca ctcatcgacc cagggaccta cagccgttca aaaaacggtt gctcgtgttc
     4261 taggattacc catgaacctt attgaggttg atgtggttag gcttggtggt ggttttgggg
     4321 gcaaggagga tcaggcttca ggatttgcag ccatggctgc acttgctgct catattaccg
     4381 gcaaaccggt aaaactggtt ctaccccgtc acgacgatat gcgaatgacc ggaaaacgtc
     4441 acccctacag cggcgatttc aggattgggc taaccgctga cctcaagata ttggcctttg
     4501 aaacaaccct gtaccaaaac ggcggcgccg cagccgacct ctcgccagct atcatggagc
     4561 gaacgctctt ccactccact ggttcttact atatccctaa cacgcgagta accgtgtata
     4621 gctgcaaaac caaccttccg cccaatactg cgtttcgtgg ttttggtggg cctcagggaa
     4681 tgtttgtaat tgaaagcgcc attgctaaag cagccgaaac cattggtgta agcgcaattg
     4741 aaatccaaca ggcaaacctg ctaaaagaga acgatgaatt ccagtacggg caaaaggccg
     4801 aaaacgtaaa cgcccgtaag tgctttgccc ttgcatcgga aaggtttgac cttgagaatc
     4861 tccgtaaatc cattgataat tacaaccaaa acaacaagta cagcaaaagg ggcttggcgg
     4921 ttatgccgct ctgctttggc atatcgttta ccaacacagc aatgaaccag gctcgcgccc
     4981 ttgttcatat ttaccaggat ggcagcgttt cagtaagcac tggagccatt gaaatgggac
     5041 aaggtgtgaa caccaaaatg gcgcaggtag catccattct gctggggatt gatattagcc
     5101 gggtaaagat tcattctacc aatacaacaa gggttgcaaa cacctcacca acggctgcca
     5161 gtagcggtgc cgacctgaac ggcaatgcac tacgaattgc cattgctgcc ctaaaaaaac
     5221 ggttgcttaa ggtagccgcc gaactactgg aatgcagcga tgatgagctg aacattgaaa
     5281 acgaaacggt tatccgtaac ggtgcaccca cagccctgaa atggaacgag ctggttaaaa
     5341 aggcacacct gaaaagggtt gcgctaactg agaacggtca ctatgccact ccggttatct
     5401 actttgataa aacaaaggag aaaggacacc cctttgccta ccatgtttat gggacaggag
     5461 ttgttacagt aacgttggac tgcatcaggg gaacctatac cattgatgat gtttacattg
     5521 tacacgactt tggcaatagc ataaacccac tgattgacct tggacaggtt gagggtgctg
     5581 tagtgcaagg gataggatgg ctaacccttg aggagttagc ctttgactcc aagggccgat
     5641 tgctttccaa tagcctatcc acctacaagg tacccgatat tcactttgca cccaaaagca
     5701 tacagtgcat cccgctgccc accaatggcc cggaattggc agtactacgt tcaaaagcca
     5761 ttggcgaacc accattcatg tacggcatag cagcctactt cgccattcac aatgccatta
     5821 aggcctttaa cccacactat agcataaagc tcactgcacc cctaacccct gaaagggttt
     5881 taatgaatct gtaccagggg aggtagggtt tggtgtttag tgtttagtgt ttagtgtttg
     5941 gttaatccaa aactttgtaa atccaataaa tcagcataat ctgcgttcta ttaagctcac
     6001 tatcaatcat caacgaacga cttcattggt taggtaaaat agtaagcaaa aagttaaagg
     6061 tccgtttgat gatttgtaat tttttttaaa ttagctatcg tataagtctt tcaattatca
     6121 gatctataca cccaaattag atgtataaga atgatttttt aggatataaa cgagttagac
     6181 gataatactg gaggttaata atatgaagaa attgaagtac tttttgatgc ctgtgttttt
     6241 tttcttcttt tttatcccac ttttctccgt gcacaagcag cggttgaaac ttacaagcag
     6301 gcaattcgct taaaacctaa ttatgtagag gcatactata aactggggat ggattatgtc
     6361 aatctcgggc gctacaccga agcggttgaa gcttacaagc aggcgattcg cttaaagcct
     6421 gattatgcag atgcacactt taatctggga ttaacttatg gccagctcgg gcgctacacc
     6481 gaagcggttg aagcttacaa gcaggcaatt cgcatagagc ctgatgatgc agaagcacac
     6541 aacaacctgg gtactgctta tgacaaactc gggcgctaca ccgaagcggt tgaagctttc
     6601 aagcaggcga ttcgcttaaa gcctgatttg gcaatggtac actataatct gggagtggct
     6661 tatttgcatg taggagataa gagttctgca tttgaagtgt acaaaattct caaagaattg
     6721 gacaaagatt tatccaacaa attgttcaac ctaatttaca aataagcgga atagaaaacg
     6781 gtaagaagta gcctaacagc atataaaagt gaaaacaaag ttttccataa ttgctcttgt
     6841 tagcgcgcgt aagcgatgtt aaatggagta taagcctaac gcagcgtttc agccggacat
     6901 aaaaaagttt ttgtacttgc ttgaacaagt ggatttttta ctacatttac tgaaagttat
     6961 cgtacggagt ggcgtatatt gcacgcgtcc gcagacgcac aagagcgagg gatgaatttt
     7021 ttatttaata tcaaactaat gatctgacaa ataggaatgt atatattgaa ggatagggtt
     7081 ttaagtaatt attttgaata atttaactta aaagtgaaac aaaaaattgc ataattcggt
     7141 tataagtgaa tacatttgca atcgaaatat ggtatgatga aggaagcatt tgcacattct
     7201 acaccgtcag gtgggttaca gatgatgaca atgcaccatc agaaacggat aggttttttg
     7261 atacctatgc tgtgcctgag catccattaa atgaaaaggc aatgcaattg tttcgattga
     7321 taaccgaaag cattggcaac agatacggag caacaaatga tttttttgac agaatagtaa
     7381 acaaagcaca agaattgcct ccaaaacaaa aacaatgggt agaagaaata aaagacttag
     7441 gcatcaattt tccactgagg ttattttgtt atcgggtaac cgaaaatatt gttattcttt
     7501 tcaacggtgg gattaaggaa tcgcaagcat cacaagaaag caaaaattta agaatgaagt
     7561 tttatgaagc acaaaccttt gtcaaaaaaa ttgaagaagc attacaatct caaatgatta
     7621 aaatctctga taacggtagg tatttacaaa actttgacgg gacaactgac ataatactat
     7681 aagacaatga gaagcaagac agtagataga ttgctgaaaa gcacacctaa agatgttgaa
     7741 atctttgtgg attggtatgc tgacttagtg gttcgcatta atcaactatt gcgtgaaaaa
     7801 aatatcagta aaaaggaact gtctgtaaag atgaataaga aaccatctga aatttccaaa
     7861 tggctaaacg gtgagcacaa tttcacctta cgttcactag caaaattatc ggctgaattg
     7921 ggagtacctt taatagaagt gacgaagaaa aaagtacaga cagaatttgt tgaagatggc
     7981 tttgtgtgca gagttcacac ttttgtgagt tatacaaaac taaacacaaa aaccactgaa
     8041 aatgtttggc aatgtgccga agaaactgaa ccctataatg aattaagcaa tgcaggataa
     8101 aaatgttttt gatccagaaa agattgcttt gattgatttc aaaatgatta aaggacaggt
     8161 gaacactcct gaaaattttg acattaacaa agttgtcgga taccatttgg acaactcctt
     8221 gcaattgagc tttaaccttg acgataaatt ggctaaagca gatttcactg ttaacgtaaa
     8281 aaccgacagc aagggcgaaa atgaaagcga agcaacagga aatttccatt taatatttat
     8341 ttacagaatt gaaaatttag aagagcttac tatactgaaa aaaaataagc gtttaaatct
     8401 aaatccgggt ttggcaaatg cactttcatc tgtaacctat tctacatcaa gagggattct
     8461 tataacccga ctgcaaggca cggctttgca aaactttgtt ttacccatta tcaacccgaa
     8521 caagttgctt cacaataagt aaaaatagat gatttggaaa tacttaagaa ttataattta
     8581 gaagatcaaa cagcaatctc aaaccatgca ctgacaatga ttcatgagga cggatagaca
     8641 gaacaccagt tgccaacatc ggtttagcac attgcagggt gcagtgacta attcgaaatg
     8701 tagttttttt ttcattcatt tgttggaata tgacagtctt gcacttttaa ttcccgtgct
     8761 attgccaaac tgcaacctgt ttccactccc acccctttca taaacccaac ctttaaaccc
     8821 ccaaagattt cactcttctg agcatctcaa attccgttgc ctggcaattt cccatcggca
     8881 ataagcctgg catactcttc gggggtgttg atgttgataa ggatttgatc attgggaacg
     8941 ggtatgcatg ccttgctaaa tcggtttaga tactctttta ggttgatatc cgggttggtt
     9001 tctgctcgtg ctgctttcgc aattcgcgat gaaattagta tgggatggcc acccctaccc
     9061 tcgtaaaccg ggtaaacata gtcggcttgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn agcaaagcct taggctgccc cattcgcccc gaggaaccgg ctgccagaat
     9241 aatggcggaa aaattttctt tcattcgctt gtatagtaat attctttgcc aaatagcaat
     9301 aaaatgctat acaaagttaa tatgcttagc catattttat agatttgtgg gaaatatcag
     9361 gatatatgag cgataagttt tcacccatac cggccaaaca gttgctggaa atcattctga
     9421 aagaacttga tacccatgga accgtttttg gataccccag cgaactgttc tttaacccga
     9481 atcaaggtaa acttcccaca accatttttg ggcaaaccat tgatacacca ataggtgttg
     9541 ccgcagggcc acatactcaa cttgctcaca acattgtaat tgcctggctc atgggagctc
     9601 ggtaccttga gctgaaaacc attcaaaccc tcgatgagct tgagattgca aaaccctgca
     9661 tcgacatgca ggatgagggg tacaactgtg agtggtcgca ggagctgagg gttgaggaga
     9721 gctttatgga atacctaaag gcatgggtaa tcattcacat cctgaaccat aagctgcagc
     9781 tgggcagtaa gcccaatatg gttttcaaca tgagcgtagg ctataacctt gagggtatta
     9841 aaaagcccaa cgttcagtgg ttcctgaata aaatgggtga tgccaccggt gagctgaacc
     9901 agatggtgca ggagctaaaa gccatatacc cagccattgg aaacatcgat attcctgcta
     9961 agctttcgga taatattacg ctatccacca tgcacgggtg tccggccaat gagattgagg
    10021 atatagcccg ctacctgcta actgaacgga gattgcatac gctggttaag ctcaacccta
    10081 cccttttggg aagctctgag ctcagagaga tactgaacaa caagctcagg ttcagaacca
    10141 acgttcccga tatcgccttt gagcacgacc taaaatatcc cgatgcccta aagctgattg
    10201 aagccctttc cgccgaagca aaaaccctta accttacctt tggggtcaag ctaaccaata
    10261 cgctggaaag cgttaacaat aaacatgttt tcccaggcga ggtggacatg atgtacatga
    10321 gcggccgggc attacacccc atttccataa accttgcccg caagctgcag aacgatttca
    10381 aaggccagtt gcttctatcg ttctcggcag gagttgatgc gtttaacgta tcaaaggtta
    10441 tgggctgcgg ttttagaacc gtaaccgtat cgtccgacct gcttaaaccc ggcggttatg
    10501 ggcgattaaa gcagtatatc gataatctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnncc atttccgaac acaaccggaa tggtgtgcga ccacctttgc cagaacaagt
    10681 gtacccgtat taactacgac aacccattac aaatacgcga cgtgaaacgc tacgttgctg
    10741 agaatgctga tatcaagcta agtcctgctc aaccaaatgg taaaagggtt gccataattg
    10801 gcgccggccc atcgggcttg tcgtgtgcat tttacctggc cattaatggc tttaaggtag
    10861 atgtttttga aagcaagacc aaggccggag gaatggttcg ctatgccata ccgggcttcc
    10921 gcttaaccga ccaggccatt gatagggata ttgaaaggat tacccaactt ggtgtaaaca
    10981 ttcattacag ccaccccgta acccgcagcg agtttagcga ccttaaaaac aagtacgatt
    11041 acgtttacat atcggctggt gcgcagaaat cggctatact tgatattgaa ggtattgatg
    11101 ccgatggtgt tattgacccc ctaaccttcc tgatgggtgt aaaaagcggt aacataaagc
    11161 agattggcaa acgtatagct ataattggcg gtgggaacac cgccatggat gcagcccgaa
    11221 cagcgcatag gcttgtgggc gataacggaa aggtaaccat agtttaccgc cgaacagtta
    11281 acgaaatgcc tgccgatgag ggtgaaatta aagcggtaat tgccgagggt gttgagttag
    11341 tggaacttgc cgcccctgaa aaaataatca cccagaacgg caaggttaaa gcattgaaat
    11401 gtagcttaat gaagctagat ggttacgata gcaagggtag accaaagcct gtaaaagttg
    11461 aagggtctga atttgaggtt gagtgcgatg tggtaattcc cgccattggg caagcccttg
    11521 atatcgattt tgttccaaat gagctcctaa aggccgatac ccgcaactat aaaacgttga
    11581 tgggtaacgt ttacattggt ggcgatgcgc ttcgtggcgc atcaacggcc ataaatgcca
    11641 ttggcgatgg tcgtaaggca gcccaggaga ttatcagcca atgcggtttt acgggaatta
    11701 gtactggtgt ccctgaaaag aaacataatt atagggatct tatgctcctg cgatccaccc
    11761 gcaagtatgg ttatgtacat aaagagctca ctgctgaaaa gagcaaaacc tttgagctgg
    11821 tgactgaaac tccggacgaa acgaccataa aagaggaaag cgcacgctgc ctacactgcg
    11881 acgaggtttg cagcatatgc acaacagttt gccccaacct agctaacttt gcgtatacca
    11941 ttgaacccgt tcgttaccaa ctcgacaaag tgctggttgg ttccgatgga acacaagagg
    12001 ttcaacccga ccattttttt gaggtaaaac aaaccgttca gatactgaat attgcaaact
    12061 tctgtaacga atgcgggaat tgctctacgt tctgcccaac cgagggcgca ccctaccagg
    12121 ataagcctaa gtttcaccta accacaaaat cgttcaagga gagcgaggat ggttacatgc
    12181 ttagcgttct gaaggacaga aatgtactaa tctttaagca aaaaggaggc attaagaccc
    12241 tatcggaaaa ccgtgatgaa tacatttacg aaaccgacca cgtttacgcc cgtttcagta
    12301 aaaatggctt taagctggtt gaggctaagg cgttaacgcc ttgcgtaaga caaattcaat
    12361 ttcagcatgc ggtagagatg agcgtaatac tacagggtgc aaaaaggtta atgggggttt
    12421 gattagggct tggtgtttag tgtttagtgt ttagtgttta gtgtttagtg ttaagtgttt
    12481 agtggttagt gcttggtggt tgtgtattga ccccctttaa actggactaa tctccaaagg
    12541 gtttagagaa gattcaaaaa aataaattat cagaaattac cattaatgcg tattctacag
    12601 cttgttactc ggttcgattt tggtggagca gagaaccacg tgcgtgagct atgtaacgag
    12661 cttgcagcaa gcaaccatca ggttattctg ctttcccgaa agggacgaca aaatgagctg
    12721 ttggataaac gagtaaaatt tatacaacta ccttcgtttg tcagctattt gatactgaca
    12781 aaggttgtaa taatagttta cctgctatta cggcataaga ttgatgtgat acatgcacac
    12841 caacgactcc caataatttc ggcatgcata gggggattta tactaagagt cccagtggtg
    12901 gctaccgttc acggccgcgt taggcacgat ttaaggtcga tagtagcaaa aaaattaaca
    12961 tcaaaaataa tctttgtgag aaatcaggca atttccaact tagacaggtt taggtctata
    13021 caaaacaaat cagtttatat acctaatggc attccgattc ctaaaaaatt accactcatt
    13081 gaaacaaata ctattggata catctgtagg attgataaaa ggcattttag agtaataaaa
    13141 ctgcttattc ttgcctgcga aaaattgaaa ttaagtcaac ctaccattaa actattatta
    13201 attggcgatg gaaataatgt agaagagtta aaagaactga taaacaaaac caatttaaaa
    13261 ttcgaaagta ctgctattga atacttgggt tttgttagaa atattgattt actgaatagt
    13321 tttccggaac ttatacttgg agttggacgt gtagctattg agggacttat tcgaggggct
    13381 actgtaattt cgataaaaaa caacaaaatg ggaagaatta ttacgccaag caattatgat
    13441 gactatgcat ttaccaattt tattaaaatt gatggctgtg ttcctactga agaatcgata
    13501 tataatgaac ttaagcttta ctttgaaaga agaaatgaat accgaaaaaa cacgtcaatc
    13561 ctagcagcga gaattgaaaa cgactttgga ataagtaaaa caaccaatag aataattgag
    13621 gtttactcaa gcaccctaaa atcaagaggt ttgcctaaca gcaaatagtt aaactcttaa
    13681 aaatcagtat gtaaaaatag ttcaacttat aaagaccatc tacaactcat acttgtaggt
    13741 ttgtttcgat tcggacataa caaatgagct gtaaaattga gttatattct ttatcactga
    13801 aaatttatga attacaaagt tgtggtagtc ctccatgtcg ttacactgcg ctttaagtaa
    13861 gaagtcccag ttaccggaaa cgtaataaca ctccataacc tcgggtaact ttttaacttc
    13921 gagctcaaaa gcatcaatca cctctttggt gtgactcgag agcgataccg agatgaaaac
    13981 cgtaagcttt ttgcctagag ccttagggtc gagtagcgtg atatactgct taatgtaacc
    14041 attcttctcc aactttttaa ttcgctcatg gatgggtgtt gttgaaaggt gtagcctttc
    14101 cgaaagctcg cgaatggtaa cacggctatt gtcctgcaaa atgttcagaa gcttgcggtc
    14161 tatttcgtca atatttctca tacttagtta agttttaggt taaaaaaatc tgtaaattgc
    14221 aatactattt ttcttatcta gtaatatttt tatctgcaaa tataatatat ttccataaaa
    14281 cgcaatatat ctaatattat tttctatatt ttacaaaata cttagaaatt taagacagag
    14341 ttattacttt tattgaaaat ttcaaaggtt atgattgaga taattgatag aattacaaac
    14401 cccaaggcat tagagaatgc cgtgaaacgt tttcgtgaga agggtattat tctacctact
    14461 tttcaccagc agcagaatcc agaacaaatt cctgataaaa tcaaggtaaa gctaaaagat
    14521 gttggcttat gggagataaa tccagtaaat ctttttcgaa tcacatggaa aaacgaacca
    14581 aaggaaaaag ggggattatt tggcaaggtt aattacttag agttacccaa ggaactaacc
    14641 ggggttaatg cccgaattat actattgctt ggcaaatggt ttccaactgg tgcgcataag
    14701 gttggggctg catacggatg ccttgcaccc cgcattatta ctggtggctt tgacccaaca
    14761 acccaaaaag cggtgtggcc atcaaccggc aactactgca ggggcggagc attcgattct
    14821 aagctaatgg gtactgagtc ggtggcaata ctacccgagg agatgagccg tgaacgcttt
    14881 acctggcttc gcgactatgt gggatctgag gttattgcaa cccccggttg cgagtcgaac
    14941 gttaaggaga tatatgataa gtgctgggaa attcgtagaa caaaacccga ttacgttatt
    15001 tttaaccagt tcgatgagtt tggcaatgcc gcatggcact ataatatcac agggaaagcc
    15061 attgaggagg tatttaacga gctgggcgga aagtcaaatc ttgccgcgta cgtttcggca
    15121 accggctctg caggaaccat tgccgcaggc gactacctgc gaaccatagc gccacacatc
    15181 aaggtggtag cctcagaggc aaagcagtgc cctaccattc tgatgaatgg ctttggcggc
    15241 caccgcatcg agggaattgg cgataagcat gtaccctgga ttcataatgt taagaacacc
    15301 gatgttataa ccgctatcga cgatgaggat tgcatgcgaa ttttcaggct cttcaatgaa
    15361 ccagaaggtg taaaatacct tgtagaatcg ggagtgcctg tagaaattgc caacaacctg
    15421 aaacttttgg gtatatcggg cattagcaat atgctctcgg ctattaaaac agccaaattc
    15481 tttgagatga cttcagagga tgtgatattc accatagcaa cggattcggc cgagatgtat
    15541 ggatcgaggc tagaggagtt aagggaggaa cgcggaagct atagccaaat ccaggccata
    15601 aaggattttg agaaatgtat cctaggtgct actaccgatt ggatgaggga gctaacctac
    15661 cacgaccgca aggccattca taacctgaaa tactttacct gggttgaaca acagggcaaa
    15721 gaaattgacg atttgaacag gctctggaac gacagggagc tttggcctaa actctttagc
    15781 caggttaaac ggtgggacga acttattgat gaatttaacg atcgggttgg tctgctaaaa
    15841 tcgctttaaa tcaatttttt atgaacggaa agttcatact tacctgctcg ggctgtggca
    15901 aaacctttgc gccatcgcct gagctttacc tttgccctga ttgcagcagg aataacgata
    15961 ctgtaaaccc accaaaggga gtgctaaaag cattatacaa ctatgaaatc ctgagtaaac
    16021 ataaggaaga ggtaatcaca ggaaattttt gggatctaat cccgctgctt aaaaaggaat
    16081 cgttacccaa gctaagggtt ggcgaaacac cattgtatag gtttgataga attgatggtg
    16141 aaacgcttac atttgagcta tacctgaagg atgattcgca aaacccaacc ttttcgttta
    16201 aggatagggc atcggctgtg gtatcggcct atgccaagga aaatggcatt agcacaatag
    16261 ttgctgcatc aaccggaaac gcaggctctt cccttgccgg catatgtgct tcgcaggagc
    16321 aaaaagccat tattatggta cccaaaagcg caccacatgc taagctcacc caaattgtga
    16381 tgtacggagc cacaattgtt ccggtaaacg gcacctacga tatggctttt gaccttagca
    16441 tggaggcaac caaagagttt ggctggtata accgaaacac agcctttaac ccgctaacca
    16501 ttgagggaaa aaagaccgtt tcgtttgagc tgtttaaaca gctcagcagg gctatacccg
    16561 attatatctt tgttcccgtt ggcgatggtg taattatttc aggggtttac aagggatacg
    16621 aagatttgct caaaataggg ttgatagatc gtatgcccac ggttgttgca gttcaggcag
    16681 ccggcagctc aaatctaatc gaaaatattg gaaaacccga ttttaccata aaacccagca
    16741 atacccttgc cgactccatc tctgtagata ttccacgaaa tttctttatg gctcaagact
    16801 acctgctcca atacaagggt gaatggctct cagtaaccga tgatgatatt gtaaatgctt
    16861 caaagatcct tgcgcgtaac accggaattt ttgctgaacc tgcagcttca acagcctttg
    16921 ctggaatgct atgctaccaa aagaatcaaa aattgcttac aaagcaaagg gttgtcgtgt
    16981 tgctcaccgg tagcggttta aaagatctca aagccgtttc atcggttatt aatattccaa
    17041 atgccattga ccctaccatt gattcgttga ggaaaaacga atggattaaa ttttagaatt
    17101 tgaaatcctt gaggtatagg ttaacggagt tttgtgtata gaatcctgaa atctgttttg
    17161 cgacttctac cctattccaa aacattttac ctttgtaaat gctatgatac gattcaactt
    17221 aaacggtaaa acggtagagt ataacggtga tataaatcgt tcgttactcg agtatttgcg
    17281 gaacgacttg cacctaaccg cagctaagga tggctgctcg ggtcaagggg tgtgcggggc
    17341 atgctcggta gaggttaacg gtagcattaa aatggcttgc cgtatcaaaa tgtccgacct
    17401 aagtgaggct atcatataca ccctggaggg gttgccaaat aatttccgcg aaatattagc
    17461 ccaccacttt gccaccaagg gagcagttca gtgcggattc tgctcgcccg gtatgataat
    17521 gcgtgccaga gggttatggc tatcgaaccc caaagcaaca cgcagcgata tcatcagggc
    17581 tatcgcgccg aatctttgca gatgcacagg ctatgtcaaa attattgatg ccattcagca
    17641 tgcctttgcc gagctaaatg gccaagcttt gccaaagaat aatacaggag ccaggattgg
    17701 cgaacgctat ccaaaatatc aagccaagga aacagcccta ggccaaaggc cttttgttga
    17761 cgatatgttt gttgatggga tgctatacgg agccttaaag ttttccgacc atccacgagc
    17821 aaaggtttta aaaattgata cctcaaaagc cttgtcggtg cagggtgtgg taaaggtaat
    17881 aaccgcaacg gatattccag gagaacgcta cattgggctg atttttcagg attggccagt
    17941 aatggttgcc ctgggcgagg ttacacgtta catcggcgat gttatagccg gagtggtggc
    18001 aacatcggag gatattgcca ggcatgccgc ttcattaatc gaggttgaat acgaggtatt
    18061 agagcctgtt accgatatcc atcaggccat aaaacccgat tcgccccagg ttcatcccga
    18121 tcggtcaaat gtgttggaaa catgcacaat tcggtttggt gaagttgata aggcttttgc
    18181 tgaagcagct tacgtttcgt ccgatattta cgaaacccag cgtattgaac atgcatttct
    18241 ggaaacagaa gctgcacttg ccctacccaa tggagatggc ataaaagtat tcagccaggg
    18301 acagggtgtt tatgttgaca gaaagcaagt agcaaaaatt ctaaacctac ccgaaagtaa
    18361 ggttaatgtg gttcaggttc aaaacggtgg tgggttcggt ggaaaagagg acttaactgt
    18421 tcagggccat gcagccctga tgagctacat aacacaaatg cccgtaaagg ttcaccttac
    18481 ccgcgatgaa agcataagaa tgcaccccaa gaggcatccg gtttggatga aaatgagcct
    18541 tgcctgcaac tccgagggga agtttactgc tcttaaactt gaggccatag gcgatacagg
    18601 agcatatgca tccgtaggga ctaaggtgat ggagcgtgtg gttggacatg ccacgggtgg
    18661 ctacaccgtt cccgctgtag atatctcggc taaaaccgtt tatacaaaca atatcccatg
    18721 tggagccatg cgtggctttg gtgttccaca ggttaccttt gccattgaaa gctgtatcga
    18781 cgacctttgc cgtcagggtg gttttgaccg ctggcaaatt cgctacgata acgcccttga
    18841 ggaaagcgcc aaaactgcaa ccggtcagga actacatggg gttggactaa agaaaaccct
    18901 tattgcagtt aaggaacaat ttaagtcggc aaagtatgca ggcatagcct gcggcctaaa
    18961 gaataccggt gtcggtaacg gaatggttga cgatagcgag gttataatca aggttattag
    19021 cccaaccagg gttgaaattc accacggatg gacggaaatg ggtcaaggcg ttcacaccat
    19081 ggccattcaa acgcttcacc atgaaactgg tattgaacca agctgtatag aggtgatagt
    19141 tgataccgat gcggagattc caactggtat gaccacttcg tcaagggcta cagccctggt
    19201 agccaacgcc cttatcaacg ctgcgcgtcc actgagagaa gatttaaaga cggccaaact
    19261 cgaaaagctg gtaggccggg tttatcgtgg taaatttgtt tgcgattgga catgcaagcc
    19321 cggcgcaaaa actgataaac cggtaataca ctttgcctat ggctatgcaa cacaggttgc
    19381 cattcttaac gaacagggca aactttcgaa aataattgct gcccacgatg cgggcaaaat
    19441 catgaatcag atgcttttcg aaggtcaaat tgagggggct gtgcatatgg gaatgggata
    19501 tgcactaacc gaggatttac ccatggagaa tggctttccc gttagcttta agtttaagga
    19561 cattgggatt atccgcgcca accaaatgcc cgaaattgaa gttattggaa ttgaagagcc
    19621 cgacccaatc ggcccattcg gtgcaaaggg tattggcgaa ataggcatgg tgccaaccgc
    19681 tgcagccata gccaatgcgc tttggacttt tgacggtata aagcgaacac gcctacccat
    19741 gatgcggaac aagtcagaat aatgtttaac caccaaattc ttacccaatg attttacttt
    19801 ccgatgccac ctatatcgac tatcaaacgc tggaaatcaa gaattgcgat atactggtta
    19861 ccaacggtaa tattgaattc catccaccca aaacatacag gggcaatgcc actgaaacca
    19921 tcaactgttc cggaaagttg gttacgcatt cgtttattgt tggccatcac catgcatact
    19981 cagcattagc ccgaggaatg ggtgcaccca aaaaatcacc tgaaaatttt tacgaaatcc
    20041 ttaaatatat ctggtggaca ctcgataaat gcctcaccac cgaaatggta gagtatagcg
    20101 gcttggttac cgctatggct tgtgcaaagg caggggctac atttgttatt gaccaccatg
    20161 cctcccctta tgcggtaaaa ggttcacttg agacacttgc caaagcattt gaaagggttg
    20221 gcctatcgca cctactctgc tacgagatta gcgataggga tggtgatgaa attgcccaac
    20281 aaggacttga ggaaaccgat agctacctga aaaactatca gggacttgtt ggcctacacg
    20341 catcgttcac cctaagcgat aagaccttgc aggcagcagc agatctaacg cagaagtata
    20401 attcgggtgt tcatatccat gtagccgaag acaaatacga ccaacaacat tgccatgaaa
    20461 cctatggtaa aactgtggtt gaaaggctta gcgactttaa cttcctaaac tctagcaaat
    20521 cgattctggt gcattgcctg cacctttccg aaaaggaacg taaccttata ggtcaatcgc
    20581 cctgctgggt agcacaaaac actgatagta acctgaacaa caacgttggc tatttcagca
    20641 gtaaaaatct gggtgaaaat atcatgctgg gcaccgatgg tatgcacagc gatatgctac
    20701 aaagcatgaa atcggcttac tttgttggcc aaaactttga ccccatgagc cctgcgcaag
    20761 catacaaacg acttagaaat gctcaccact acatagccca aaatggcttc agcggcgatg
    20821 gccccgataa ccttgtggtt ctcgattacg attcccccac ccccgttaac gctgaaaata
    20881 ttcctggaca ctttatttat ggtatcaacc agtcgcatat agaacatgtt atttccaatg
    20941 gaaaactgat agtcaaaagc agagcgataa ccaccgttga tcaggaaatc attcttaaag
    21001 aatcgcaaaa attagcgcta gagctttgga aaaggatgag aggggaagga taggataaag
    21061 gataaaggtt aaaggttaaa ggttaaaggt taaaggttaa aggataatgg ttaaaggatg
    21121 atggttaaag gttgtctagt cctgaaatat tgggatagat tttgcggaaa cgtcaatcag
    21181 tcatgctgag ctcacgatgg tatctctttg ctcatttttc cacttggcgt tgcatgagat
    21241 cctgcgcact tcttggtatg acaaatggag ttgatggttg attgtcannn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nnnnnnnnnn nnnnnnnagc aataaattcc tctttttatg cttatatttg
    21421 ccctttaaaa caaatttaga tgaatcaaca agtactggaa tggattgggt atagcgcatc
    21481 ggtaatcatt gcgctatcaa tgacaatgag ttcgatagtt aagttccgtt ggataaacct
    21541 aacaggagca ttgatgttct caatttacgg ttttctgatt ggagcatggc ctgtgggcat
    21601 tttaaacggt tttattgtgc ttaccgatgc ttactaccta tttcaaatct actcgaaaaa
    21661 agaggtgttt gagatacttg aggttcgccc cgagaaccgt tatctgatgc gtttcctcga
    21721 tttccatcaa aaggatatac atcgtttctt tcctggtttt gtctataccc ccactgaaaa
    21781 tacagttagc ttcctcattc tgcgtaacat ggcagttgct ggggtattca ttgcccaacg
    21841 caacgaaaca aaagcgcttg aggtagagct tgattacgtt ttacctgaat accgcgactt
    21901 taagaatggc aagtttgttt actactggct tagccagaaa tttaccgaaa gcgggtatac
    21961 ccgggtgtgg gcaaaggcat gcaataagag ccacaaaaaa tatttgcaac atttaggctt
    22021 tactgaatcg ggcggtaacc attttctgaa aatacttgaa taagagtagg aattgagaat
    22081 attaattaca aaaggtagga ttgtaacatc ggttggcgag caaattgccg acctgttggt
    22141 tgttgatggg aagatagcgg ccattggaaa ccagctagaa gctaatgctg aaactacaat
    22201 caacgcacag ggtatgctgg tgctgcccgg gggtatcgac ccacatgttc atttgcacct
    22261 acctacaccc gccggttttt catcggacga ctttcattcg ggcagcattg ctgcccttat
    22321 gggcggaaca acaaccatta tcgattttgt gaccccatca cggggtcaat caatggttga
    22381 agcgctaaaa gcacgattgg atgaggctgc ttgctcgcta gttgattaca catttcatgt
    22441 gagccctatt gaatggaata aaaatactcc caaagagatt gaggaatgca ttgaaatggg
    22501 atttccatcg tttaaagttt acatggccta taaaagttcc gttggaattg atgacaatgc
    22561 cttataccat gtaatgcata cagtggctca gaatggtggt atggttactg ctcactgcga
    22621 gatgggcgat gatatcgata ctttaagaga tttttatgcc agccagggtc ttacagctcc
    22681 aaagtaccac atgctctcgc gtccacctcg atttgaagcg ttagcggtta aacgtgctat
    22741 cgaccttgcc gatcaaaccc agtgccccct ttacattgtt cacgtttcgg cggctgaatc
    22801 ggttaaacat attattcaag cccaaaaaag cggccaaccg ctttatgccg aaacctgccc
    22861 tcagtatctt ctactcgatg aagccctcta cgactccagt ttacccgaag caataaagta
    22921 tgttataagc ccaccgctca ggcaaagtat tgacagagat accctctgga atgccatcaa
    22981 taatggtaat attctaacgg ttggaactga ccattgccct ttcagtcttg agcaaaagat
    23041 ggtgggcaag gacgatttcc gcaaaatacc aaacggagca ggcgggattg aacataggct
    23101 tgcgctactg tacacttttg gtgcattaac cggtaagcta agccttagca aattggttga
    23161 tgtttttgca acacaaccgg ctaaaatatt tggactttac cctcaaaagg gtgaacttgc
    23221 cattggtgcc gatgccgata ttgtgatttg gaatccaaac gtaaaaggct caatttcttc
    23281 aagtaaacat cactcaaagt ccgacataag catttatgaa ggctttgaga ctactggtga
    23341 ggttgctttc gtaatcaaaa acggaattat tgcagttgat aacggaaaat tgagcgaaaa
    23401 tctgcccagc ggtcaactac ttaaacgccg atctacgaaa acaacaggcc actactacaa
    23461 aagaatggat aattcaggtt cctacaattg tggtagggtt tgaaacaggt taattaaagc
    23521 caagttataa gatactcatc aaactcagca cctttgcttt cctccttaac taagttaacc
    23581 tgaaccccat acaccttgca ttttctcact agggtatcga gcaaccccac ctgaatatgc
    23641 gtgttaaata gttgggtttt tcgcatttga atggacttat cagtgtgtga aactatttcc
    23701 caacctctcc gtttcggatc ttttacacct ttctgcgcta gcaaaaccaa tgctttcatt
    23761 acatccaacg gcttactctc ggtattaacc atgttggttt caagcattaa actgtaggtt
    23821 gattcaccaa tccggcggcc aattttaatt aggttaaatt gtcccagctt cttttcaatc
    23881 cagctacata gctccacgta aactttgaat ggaattttgt tggtaggttt cgacaggtca
    23941 taaccactaa ctatctgaaa taaatcgggg taatcggctt ttttatcctg ataacagcta
    24001 attatatcat tgatagccca tgcatcaatg aatatctcct ctatcaactc tttcattttt
    24061 atatcttttt atcacaataa aaataataca tggcatagtt taataccaac attcacatca
    24121 tttttagaaa ttataaacaa taattattta gtctttagaa tattcaaaca aatgattagt
    24181 caaaagtata attctaatag atatacctga agggaaaggc atattttagt ataacattta
    24241 ttctgttaaa ctacataata aagcaaaaaa aaacagcacc ttaaggtgct gctgaaatat
    24301 aaaatgggct actactttat aactcttggt gtgttccttt caataagttt cattagaata
    24361 tcagcgggat tttggaacct attggtaagc atcatagcag caataatata cggtttgaaa
    24421 cccgcttcct ggtaagtttt aatgcggtaa cgctcaaagg tttccttact tacctcaccc
    24481 tgaatgcacg attcgcccga aatatcggct ggtaggcagt gcatgtaaag agcatcggaa
    24541 ttcctggtta ccttgcgaac ctgctcattg tactcccaat ccttgtactt ggcattgtta
    24601 gccaggcaaa cctgctcgag ctctttaagg ccattcttat cgccattcat aagcagctga
    24661 gtgcgttgct gcatgatgtg gaatggcgcc cagctttttg ggtaaacaat atcggcatcg
    24721 cgcatggcat cggtcatgct gtggctaacc ttaaagctac ctccgctttg agtggcattt
    24781 tttctggcaa tttcaacaat ttcaggaata aggtcgtagc cctctgggta ggctaactcc
    24841 acattcatgc caaaacgggt catcagagca ataataccct gtggaaccga tagtggtttg
    24901 ccatagctgg gcgagtatgc ccagctcatc acaatctttt tgccacgcaa agcatccagt
    24961 gaaccatact cattggcaag gtgaagcagg tcagccattg actgggttgg gtgatccata
    25021 tcgcattgca ggttcacaat accaggccta acaggcaaaa cccccttagt aaaaccttca
    25081 tcgagtgctt cgccaacctg gcgcatatac ttgtttcctt cacccaggaa catgtcatcg
    25141 cgaataccaa tgaaatcgga aaggaacgaa atcatgttag cggtttcacg aacggtttcg
    25201 ccgtgcgaaa tctgcgattt ctcctcatcc aactcctgaa ctgcaagtcc caacagattt
    25261 gcagccgagg caaacgaaaa acgagttctg gtggacttat ccctaaagtt tgaaatagcc
    25321 aagcctgaat cgaatatgcg gggcgaaatg ttttccttac gcatctcttt aagaatggaa
    25381 gcaacctgta gcacaacctt aaggtcgtcg tcgctcttct cccatgtgag taaaaaatct
    25441 ttaccgctaa ggtctgattt gtagtgattt aactccgcta ttttcttgat taaatccata
    25501 tttcaaagtt tttattcggt tttaactatg ttagctaatt aggcgtgggt aacaaattta
    25561 aaatgaagtt acagcatatt ttgtaataca acaatcgaaa aacctgaatc aatgtcgttt
    25621 ggataataag tcagactctc cctttgtcat cctgagtgaa acgaaggatc tcgattaatc
    25681 tattgttttg agatgtttcg ctccgctcaa catgacaatt aaccttaact aaacgacttt
    25741 gatacctgat tcacaattca atttgtgcaa tgctttacct aaatctcata tgcgaaggcg
    25801 gcataaaagg cagtagctgc caccaaatcg ctaatgggaa ccttctcatt gggagcatga
    25861 gctaacacct cattgcctgg gccaaaacca attgtaggaa ttttataggt tccacaggtc
    25921 atgataccat ttgtggaaaa ggtccactta tcaacctttg gagcattgcc aaaaagcttc
    25981 tggtaggtgc ttacgccttt ctgcacaatc tcactatcct cgggcatttt ccacgttggg
    26041 tagtattttt ccatgccgta ttccagtcct gtataggcct tctcgctata gtttagcact
    26101 tccaccttgg cattcatgcc tttaatcagc tcttcaatct cggcaacagc gctctccttg
    26161 gtttcgcccc aggtaaggcg acggtctaga tgaatgcggg catagtcgga aactgcgcat
    26221 agcgaaggac tactggaggt gaactccgta acagtgatgc ttccttttcc cagaaactca
    26281 tcgaatttta gtcgttcgtt tagtttttct acctccaagg ccacctttga ggccatgtaa
    26341 atggcatttt tccctctttc gggtgctgag ccatgagcag aaacaccgta aaaatgaacc
    26401 tgcatttcca ttctaccgcg gtgacccctg tatatattga ggtttgtagg ctcagtgctt
    26461 ataacaaaat cgggtcttat tttatcctcc tccacaatgt acttccagca aagtccgtcg
    26521 caatcctctt ccattacgct acctacaaag tatatggtta aatccctgtt aaaaccaagt
    26581 tccttaagta ttttgccagc cgtcacaaat gcagcagggc cgccttcctg gtcaactgtt
    26641 ccgcgtccat gtacataccc atcgcgaatt tcgcctccca agggatcgaa ctcccagtta
    26701 tccttattgc caatatcaac cgtatccatg tggccatcga tggcaagaat acgttttcca
    26761 tttccaatac gaccaattac attcccaagc ccatcgataa aaacctcgtc aaagccagcc
    26821 tcttccattt gcctctttag ctccagctgc acgtcgcgct cctgcgcact caaggattta
    26881 atctgaacca atcgggacag attacgtgca gtataatcgg catatttctc cgataactcc
    26941 tttatcttac tatatacgtc gctcatttta tgtagatttt agtactcaaa attattttta
    27001 attctacaca ttaaagcaat gagtattgat aatttttgtg ataccaatta aatatagata
    27061 ttttttctat atattacaat tttattatgc ctttaatctt cgttataact aaattgaaag
    27121 atatttttgc ctaaaaaaat ccttagagaa tatcttaata aatttacaaa aaattaaaca
    27181 tgggtatgat ttggcaattt ataaactcaa aaattgagca gaatattgac gtttacctaa
    27241 ttaccgttgc cgaaacacag ggaagtagcc ctggcaaggt tgggtttaaa atggctgttg
    27301 ccgccgatgg tagcctatac ggctcaatag gtggaggtgt aatggagttt aacctggtag
    27361 aaatggcacg caagaacctg gcagagggat ataaacaaaa cttcttgaag cggcaggttc
    27421 actcacacga tgcagctgaa gataaatcgg ggatgatatg ttccggcgag caaacacaaa
    27481 tcttcagcct tttttcaaaa gccgattcag aggttttagc taaagttatt gagcttgaaa
    27541 gtgaaaaccg tccgggaaaa attgttttat caaccagtgg cgcggaggta tacgagggtg
    27601 aatcgcatat tggcattaaa ggaattgagt ttcaaaagag cgattcaagc ttccgctaca
    27661 gcgagccttt aggggtaaaa gaaactgttt acatatttgg tgccggtcat ataagcgttc
    27721 cgctttcgca aattctcggt ctgcttgact ttagggttga attgttcgat aacaggcagg
    27781 gtttaagcac ctttgagggt aatgtctggg cccacaaaaa acagattgtt gactacaagc
    27841 aggtatcaca ccttttacct ggaaacgata gaagttacgt tgtgataatg acctttgggc
    27901 ataagttcga tgagatagta ctaaagcagc taatcggtaa acctttgaag tacattggta
    27961 tgataggcag caaaagcaag gtgaaaacca tatttgagag ccttattgcg gaaggttatg
    28021 gcaaggagaa tgtggaaagg gtttgctcac ccattggatt acccattggt ggacaaaccc
    28081 ctgctgagat tgctgtgagc attgctgccc aaatagttga atggcgcaat aaacgttaaa
    28141 tttactgctc aataatggtg gcgccaacca gtgttttaat ctttttctgt tcaggacttc
    28201 cggtataggt taggctacca ccggtactaa cattagcttc aagaaccttt gatgcgtaaa
    28261 ccttagcgct agcacctgta tttactttta cgtatgtttt tggcgcaatt aattcaattg
    28321 ccgttaaaac tcctccggtg ttaacttttg cctcatagct gctaaccttg ccccttatgc
    28381 gtagggttga accctgtcca acagttaaat cggcagtctc agccaatact gcgccatcaa
    28441 actcggagct ggtattaatg ttaattgaaa tattagttgc tttgagggta tcctgaaggc
    28501 ttactgcagc cgctgccgat accgatatct ctcttaattc cctgtaggtt atgtaaacag
    28561 tggcgcttac cctatcataa agttttccct tgagctttac ctcaagtgta ttgccattta
    28621 catctagcgc aatgtccgat gcaggaatgc tttgtgtttc aaccttggca ttaagggaat
    28681 cgcctttaac cagaaatacc ttaaattcac cccagacccg aagtttatcg aactccttta
    28741 acgagtaaac tgcggtttgc tgtgcataac ctattgccga taccagtgaa acactcaggg
    28801 ctagaattat ttttttcata gttatttgtt ttaatccata ttgtcaaaaa tagcaatcaa
    28861 attccattcc aaatagcaat taaccttttt tatcggtcga acaaaaggag actatttatt
    28921 gtttttacca aaagactaaa caaagtgaaa acttacatat caatattgat tacagtaatt
    28981 tcattaagcg cttgcgctca aaacattaag gatagtacta tgaaaagtga tgatttacgt
    29041 aataggttaa ccgatataca gttttacgta acccagcaaa aaggaactga aaggccattt
    29101 actggcgagt actggaatca ttttgaaccg ggtttgtatg tatgcgtttg ctgtggggct
    29161 gagcttttcc agagcgacac aaaatttcat tccagctgcg gatggccaag tttctttgat
    29221 agcaagtttg tagaaaatat aaagtttcag aaagatttaa gccatggtat ggtgcgaacc
    29281 gaagtgcttt gcaaaaattg cggtgcacac ctggggcatg tattcaacga tggccctgag
    29341 ccaaccggta ttaggtactg tatcaactca gcttcgttaa aatttatacc taacaaaaaa
    29401 gaatagttca tgggcagcac cacaaaatat atggcaatgt ttccacttcc ggtgtttcta
    29461 ttaccgggtg aggaaattcc gttgcgtatc tttgaacccc gttacaagca gttgattaat
    29521 gaatgcaatg aaactggcga cacctttggt ataccatatg tgagtaacga caatattaaa
    29581 tcctacggta gcgaggttga actggttaac attgtagcca agaatagcct aggtgagatg
    29641 gttattctta ttaaggcaat tgcaaacttt caccttttaa accttaaaga gaaactaccc
    29701 gataaacttt atggtggtgg aataattgag tatattgacg ataggttcac tacaaccaat
    29761 ccagagctgg tagtacttgt taagaagctg aaccttaacc ttgaccctat aatgggctcg
    29821 cttattactg gggactcagt aaaccttatg aatgttgcaa aagccataca actcgattct
    29881 gacgaaaaat tcgcatttta ctccctacga aacgagaagc aaatggttaa ctacctgata
    29941 aaaaggctaa ggttcattga gcatatccaa ttgcaggaaa aattgctgga aaataatttt
    30001 agcctaaact aatattttcc tttatctaca ttcagcaggt tatggtctgg aatggattta
    30061 atatacttgt taaaaatcct caagaagaac ctatcgacaa cataagggaa acgggcgcct
    30121 ttccagtgac tctgattgga agtaaatgtt aaggtgtagc catcggggcg cgaaataaca
    30181 agtgccgatg tgcctgccat ggaacccgag cgccatagcg ttccatcgtc cctaacccaa
    30241 cgccaaccca acggctgaaa cttcccattg tatgttttct tcatgatatc gacagactgc
    30301 tttgagagaa tgtcaggaaa atcgtttctg ccatcaacag ccattacaaa ccgggttagg
    30361 ctaatgggcg aaatcaccca gcctccggct gcacccagtg cacgcacatc gtttccgccc
    30421 ctcgatttta gaacactctg ctttgagcca tcaaaggaaa taaccttttc agcctctggc
    30481 acctcgtagt atctaacttc gttatcgtac cttaaactat cccagttatt ggccaaatgg
    30541 gcatcgtaaa tatttaatgg tttaaaaatc tcgttcttta catagctctc ataatccatt
    30601 cccgaggcct tttcaattac cctttgcagt attacaaatc caatattact gtatgagcta
    30661 tgacttccgg gctgaaagtg aagtcgtttt gatagggcaa atacaatata gtcatcaaca
    30721 tccaaaggta aatccttatg aagctcgcga gcaataacat ctttaataaa aaggtgatcg
    30781 ccccaccggg gagtccaacc gcccgagtgg tttaataggt tatgaacggt tatctcctct
    30841 accctcttat ccttatagtt caggaacttt tcatcgttta gaataccatc ggcgccaaaa
    30901 accctgtcat taagcctcag cttaccctcc tcaacaagtt tcataacggc tgtagtcgtt
    30961 ataagtttgg aaacgctagc cacacgcata gtattatagg gctgcatagc caggccttcc
    31021 tctttattgg ccatgccaaa accacgtgcg taaataatct tacccttatg ggcaatgcaa
    31081 aaggttgcac caaccaaatc ccatttgttc acaaactgct caaactggtg attgatagat
    31141 gaatattctt tgctgttagt aagagcattg tttacactca gcaaatcaac ctttgcgtat
    31201 tttgatgact cagcaatagg taatgccaga gtcctattat tttttttcag gtagatacca
    31261 aaggaaaaaa ctaagaaacc aattagaatt gccgaaagta ggattatcaa tttccttttg
    31321 ttaaccattg ctacttatat gaaactttgc gctgcgaaga tataaaaaac atattagttg
    31381 atggttgata gttgatagtt gatagttgat ggttgatggt tgatggttga cagttgatgg
    31441 ttgaagattg ataatgacaa aaaaaatgca aaatactcat atgtggttga ttaccatata
    31501 tttggatttt atgaaacacc aaatactata aaccaaacac caaacacgaa acacgaaaca
    31561 ctaaacacca ttccccctga tcacagaaca cccaatgctt tactatcgct taacagttcg
    31621 cataaactct tcaagtagct ggcaaaaccg gtcaatatcc tgttcggtag tgtcccatga
    31681 ggtcatccaa cgcacctcgg aacgatcctc gtcccaaaca taaaagaagt attcactttg
    31741 aaggggatgg atgagttctg gtggaacgat tgcaaaaacg gcgttagcct gaacaggctg
    31801 agtgattgta acgccctgta tagccgaaac ctttttggcg agcagctgag ccattgcatt
    31861 agcatgacga gcggttgagt cccactgacc atcctcaagg taggctaaga actgggccga
    31921 tatatacctc atttttgaag ccaactgaaa gccctgcttg cgggtgtaca tgaaatcatg
    31981 cgctaactgt tgattgaaaa agacaatggc ctcgccatac atcatgccat tcttggttcc
    32041 tccaaaggag agaacatcaa cgcctgaatc gcgggtaaat tccctgaaac cccttccgag
    32101 cgacacagca gcatttgcca aacgagcacc atccatatgc aaaagcatgt tatgggagtg
    32161 agcaaaatcg gccagggtct ttatctcatc aacggtataa acagttccaa gctcactaac
    32221 ctgcgatata gaaataactt taggttgaga gtgatgttga aaaccgaaac cgtgcatatg
    32281 ctttttaata ccgtcaatgg ttaacttacc gtttacggtt ggaacgctta acagcttaca
    32341 cccggtaaac ctttcaggtg caccacactc atctacgttg atatgggcag tttcagcaca
    32401 aattatggca ttgtggggtt gtgttacagc ctttagtcca agaacattgg ccgcagagcc
    32461 aataaaaaca aagaaaacct caatatcgtt gccaaaaatc tgcttgaatc tatcaattgc
    32521 tttctgcgta attggatcat caccataagc aatggtgtga ccatgattta catcgttaat
    32581 tgcttcaagt attctagggt gtacacccga attattatcg ctagcaaatc ctcgtgaaaa
    32641 cataatgtag attttttcgg taaagttaac ttttttatgg gttagcagcc attacgattt
    32701 gaattttcgg atcatttggt aatcaatata gtatagaatt ttgatggaaa aactattatt
    32761 ctgtggacta ttcaaaatcc cttcaatgtt tttaaagtag aggtactggg gattatctaa
    32821 cagctcttcg gcctgatttt tccatgcaaa aaccagttcg ctaccaggtg caaagtgcca
    32881 ttggtaaaac atatcgatgt tgaaaaggtt atagttgcgg tttgccggat tggttattga
    32941 attggtttgc tctagcaagc cgtcattttt taaggtaaaa aactcattgt acttgaagtg
    33001 ctttatgtag tggcgagctc taatgcttaa aaatgatttg gctgtaaaag cgagctgaat
    33061 atttagagta tttgttattg taaccatttt cctgctacca aaaaatatgt ttgaaccatc
    33121 gtaatcgtag tatccaatgt tgttgcgttc cgactgatat gcccagctgt agatggttaa
    33181 gagtttatcg gaaaatctta ttcgagggct aatgctatac atgtaaccct tttgattgta
    33241 catatcagcg taccaaaacg agaatctatg atccagtgca acagtcttac gataatcggg
    33301 tgaaccccac cactgaagcc ataaaacatg aggtgtaaga aatttgtgcc cctcaaccct
    33361 tggttcatag taatcataac cataacgggg attatacgat gcattaaagc ctccggtaaa
    33421 gttgttggtg ttcgtaaact ttgcctgaaa ctcaatttct tggctttgga aagctttagg
    33481 agtatataac cgactgtttg agaactctag ctgagcctca aagttcaaaa gcctccagaa
    33541 tggctcaaaa actttataag caagggccac gcccgaggag agctcattgg ggctacgcag
    33601 gtagcctaaa tcatttgggt tataatgctc actctcgatg ttagcccaaa tatacccctg
    33661 aatatttcca ctaatttttg aaattgacag gttagtcttg aatccatgct gaaccgaatc
    33721 gacaaaacgt tggcttagtg ccccaacccc actaatgcca aaggattgtt ttttattttt
    33781 tagattaaac tccgtagcag taacatttgc gataaagcta tcgacaggtc ggtaaacatt
    33841 ggtatttatt aagcttacat acgagttatt cggtaaattc tggtcgagta cagtaatgtt
    33901 atagttggtg aaaccctgcg ttaacagctt tctcctttta cctgaaacag tatcgagtat
    33961 ttcagcatag gtattttcgg tcatggcatt gaaaaaacca aaaccaaggc cattggcaaa
    34021 acgccccgat agcttactgg cattaaccat tttggtctca agtggattct tatcaacaat
    34081 ctcgtttgtt ttcagcgaat cgtaagctgc atcgtaaaag gcaggggttc cgcctatacg
    34141 tcgggagtag aaaatgcctg ccttcccaaa aagttcagtt ccctcagtaa agaactgacg
    34201 ggcctcatcg tacttaacct caaaaggagt aaggtttagc accttatcgt cggaacggac
    34261 ctggccaaaa tcaggaatga gggttacatc aagggtgaaa ctctgggcca agcccaactt
    34321 taagtccatg ccacctgata ctcttgaatc caatcccttt gtacctgcat actggtcaac
    34381 ataaccggag atatagggca aaaacgacaa ccgaagtggt ggtttaactc ccgaaatccc
    34441 tttaagaatg ccagactggg ttataaaacc cgcttgagct ttgtcaacaa agttccaggt
    34501 gctcacctca tttttacgct tgattaaccg tgaaaaattg attccccata cctgattaac
    34561 attctttgag aatcgtaaag cgctgtaagg tattttcatc tccacgatcc agcccaagct
    34621 atcgaaacct acagcacttt cccaaaccgc attccagttt ctatcgcttt ggtttgaggt
    34681 aattttaata tcggtttgaa cgcccgaagc cgaaacggtg aactgtaaat agtttactcc
    34741 atcgttatat gtactcaggt aaacactgaa agcatcggat cgcaacctat cgctgttatc
    34801 gcgctgtcca agttccctga aaatgctatc gggggaggta tcgaaaagtc gggctccaat
    34861 gtatatggca aaatcgtcgt atagtacact aacctctgtc tgttgcgatg ccttttggcc
    34921 attgtatggc tcataaatgg taaaattgtt ggcaaaatcg gatttacccc aaacattttc
    34981 atcgagccta ccattaatat aaatgtgttc agtagcccta atggcataaa tttcacgtgc
    35041 aatccttaac gagtccttat tacgacctcc taaagcaata tttcccacta aaaaagaaag
    35101 ggaaaaaagc aaaatccacc ttttcattcg taaaaaaatc ccagtttgtt tcttacatgt
    35161 atgagtttaa aaggtaaaaa tggtttaagt cattactaaa aaaaataccc catgatgggg
    35221 tattgctatt aggagtatta ttttgtaaat gggttttact ttactctgga tgattccctt
    35281 acaataagtt gagttttgag tatttcagtg acaggaggtt caggttttga ctgggtaatc
    35341 ctacggataa gttgctccat ggccttttta cccatctcaa atccgggttg gttaacggtg
    35401 gtaagagtgg gctcaaccat tagagaagtt tggtcgtcgc caaatccaac aacagcaata
    35461 tctgttggaa tcttataccc gttccgcagt aacaccttca tggcacctgc agcagtaaaa
    35521 tcgtttacag tgaaaattga gtcgaacttt aacccgctat caataagctt ttgggtttca
    35581 acaagggccg attgaaaggt gtcgcatgga ataatgagtc gctcgtcaat aactacccca
    35641 ttgtcgcgca tggcctttaa ataaccgtta aggcggtttt gaccaattaa cctgttttgc
    35701 ggtcctgcaa aatgaacaat ttttcgacgg ccctgaacaa tgaggtgttc agtagcctgg
    35761 taagcgccat caaaatcatc aataattacc ttatcggcct gaatttcatc aacagccctg
    35821 tcgaaaaaga caatggggat accctcctcc tcaaacctat ggaagtggtc gtagttagcc
    35881 gtttcctttg ataccgaaac taaaatacca tcaataatgc catttaggaa agtatcgcat
    35941 gcagctacct cgcgggtata gtcctcagca gattgactga tcatcacgct gtaaccatgt
    36001 tcgttagcca cctcttcaat accactgata accgatgaaa aaaagtagtg cacaatttca
    36061 ggaataataa ccccaattac gttgctacgc tgattgcgaa ggctgagggc aataatgttg
    36121 ggtttgtagt tgtactttgc ggcaagctcg ttaaccaacc gcttggtttc ctggctgatg
    36181 tcggggtggt cctttagggc acgcgaaacg gttgatgggc taattcccag ctcgcgggca
    36241 atgtccttga tggtaacctg gctcgatttc attttcaaaa catttgaact aattgataaa
    36301 tagattgtta tttacttatt gctattgtta cattcctaac aaatatacta ctaaaaacaa
    36361 gcaataaaag ccactcgccc aatgtaatat ttttttatca aaatacaaac gtttgcggta
    36421 tcgtttgcat aaaaaacagg cctaaaatca atgtttttcc ttgtttccag tgttaaaatt
    36481 ttgttaattg tgaaagtttt gatgtagtag ttttaatgtt taacaaatta aaaaccaact
    36541 caaaataaaa tatttgccat gaatagactt aaacagtttc taattatttt agtagcattg
    36601 ctattaggga acttatcctt aatggcacaa gaaatttccg ttactgggaa agtaactgat
    36661 gctgctgatg gtactcctat gattggagta gctattgttg ttaaaggaac aaccacaggt
    36721 acaattacaa atgttgatgg agaattctcc attaaatgta aaggtaacga tgttcttgtt
    36781 ttttcatttg taggttataa tacgcaggaa gttgctgtag gcagcagaag tgtaataaac
    36841 gtgcaaatga agcaggaatc aaaggaaatt gatgaggtta ttgttattgg gtatggcgtt
    36901 caaaagaaaa gcgaccgtac aggttctgta gtaaatgtta aagctgaaga gttaaataga
    36961 ggttcgctta ccgaccccat tcaggcaatg cagggtaagg tagcaggcgt aaacgtgtcg
    37021 aaaaaagggg gtgaccccaa ctccggcttt gctgttcaaa tccgaggcgc tgcaggtttt
    37081 gcatcaaaca cccaacccct ttatgtaatt gatggcgttc caggagctga tcctaccact
    37141 atttctcctg acgatattga atcgttcaac gtccttaagg atgcttcatc aggtgctatt
    37201 tatggctcaa gggcatcgaa tggggttatt atcattacca caaagaaagc cgataaggct
    37261 aaaggtgcta ccattgatta caatggttac gtatcggccg atcaaaccag taagcgttta
    37321 gaatttcttt cggctgctga tattcggaag tatgttaccg ataataacat tcctaatttt
    37381 gctgataatg gtgcaaatac taactggcag gatgagattt taaaaacagg tatttcacaa
    37441 tcccattcaa tagccgtttc aaattctacc gacaatcttt catatcgctt ctcatacaat
    37501 catacctaca acgatggtgt agttattcaa agtaacaagc aacgcgatat tgctcgattc
    37561 aacttaaccc aaaaagcttt aaacgatatt attacaattg atgcatcaat tgctgggaca
    37621 tttgaaaaga acaactatgt gaattatgga ggtggtatgg gttcatccaa tattttctac
    37681 caaatgtacc agcgcaaccc
//
LOCUS       DAOOWJ010000010        37261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1149_length_37261_cov_7.121486, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000010 DAOOWJ010000000
VERSION     DAOOWJ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 37261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37261
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1149_length_37261_cov_7.121486"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLW22_02040"
     CDS             complement(<1..208)
                     /locus_tag="PLW22_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73513.1"
                     /translation="MAKKPEELTDNELLAELQRRFELNKKALDELKVLNAELKQVNKK
                     LEESEALKSHFISNITNEIINPFTS"
     gene            complement(220..1074)
                     /locus_tag="PLW22_02045"
     CDS             complement(220..1074)
                     /locus_tag="PLW22_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOU73514.1"
                     /translation="MQANEFVDYYKLQLTDDEFKRLSALIYKESGIKLPPIKKVMLQC
                     RLQKRLKHLNIASFKAYFDYLFSREGFDSEIIHMLDVVSTNKTDFFREPAHFEFLTTE
                     LLPAYYGANKSSIFKVWSAGCSSGEEPYTLAMVLFEFAETHPGFNFSIFATDISTRIL
                     QTAIDAIYKEEKVGAIPLTLKRKYMLRSKDRANPTIKIAPHVRSKVTFARLNLMDSSY
                     DTKDLFDVVFCRNVLIYFDRNTQEAVINKLCQRIKPNGYLILGHSESILNMDVPLKQL
                     KPTIYRKV"
     gene            complement(1086..1601)
                     /locus_tag="PLW22_02050"
     CDS             complement(1086..1601)
                     /locus_tag="PLW22_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOU73515.1"
                     /translation="MDTENKNQIKSYLTFKLGNEEFAAHVSKVLNILEMTPITKVPKA
                     PDYLKGVINLRGAVLPVIDTRIKFGMPETEYTNNTCIIVLDIDVDGESVHVGAIVDSV
                     QAVLEIDNSQIMPLPTLGSRYKSEFIIGMAKVEERFIIILNMDAIFSVDEITSIVDSN
                     QSVAEGTDAQQ"
     gene            complement(1621..3648)
                     /locus_tag="PLW22_02055"
     CDS             complement(1621..3648)
                     /locus_tag="PLW22_02055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOU73516.1"
                     /translation="MDSFRKKFIEEAAELIDKLEVSLLELEKSPNDEALIQQVFRVMH
                     TLKGNSAMFGFDLIDSFTHNLETIYDMIRNGQLEVSKDILNITFSSVDHLKAMLDEQN
                     YEDPDFKTVHNGLMGKINRIINPSNAETEAESAKSSNEKSESLSTYYILFEPNENIFK
                     NGTNPLYLLDELCSLGEHKVFAHFNRLPGIKEITPNLCYTYWEVLLTTDQDVNAIHDV
                     FIFVESDSKLEIQKLAEENLLADKLFVEEVSQLAEVQKDVGLVTIQRLATKAAARINK
                     EKAKQFTKERIATKDKTAASIRVSSDKLDHLMNLVSELVTTQARLSLFAEQTNVPGLA
                     PIVENVHKLTRQLRDIAFSIVLIPIENLFGRFQRLVRDLSAELHKEVDFIVEGADTEL
                     DKTIIENLSDPLMHIIRNSMDHGIEDTRVRIANGKPAKGKIHLKAFYSGASVIIQVSD
                     DGAGIDPEIIHAKAISKGLVPSDKKMSKKEILDLIFLPGFSTAKKVTDVSGRGVGMDV
                     VKKKISDIRGEVEVESEVGKGTTITIKLPLTLSIIDGLLVSIDSSPFVIPLSSIDKIY
                     AVDKESIYSSFNNLVTLDGTQIPFFSLRKEFNLQPSSEELEQVVVVNFEDNRVGLVVD
                     YVIGEYQAVLKPLGKHYKSHDIFSGATILGDGTVALVMDTNKIIKMFATSK"
     gene            complement(3648..4013)
                     /locus_tag="PLW22_02060"
     CDS             complement(3648..4013)
                     /locus_tag="PLW22_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU73517.1"
                     /translation="MAKTILIVDDSDSIREVVRFTLENEGYNVLVGVDGKDALKHLDG
                     KTIDLVITDLHMPEMDGIELIKHIRAMPEYERIPILFLTTESQTSKKMEAKDAGATGW
                     IIKPFVPAKLISAINKVIR"
     gene            complement(4035..4343)
                     /locus_tag="PLW22_02065"
     CDS             complement(4035..4343)
                     /locus_tag="PLW22_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73518.1"
                     /translation="MSKQEKNFSIERQKGSSLVILSGDLSLNNISDIKSELIKLFNEA
                     KPTDLLIKNAEVIDLGFIQLVRSFNFSMKERGIKSTVKFELTADQSTILERSGIKSEY
                     "
     gene            complement(4357..6090)
                     /locus_tag="PLW22_02070"
     CDS             complement(4357..6090)
                     /locus_tag="PLW22_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73519.1"
                     /translation="MKKIKEISEQFAVIDSLIVEILNCAAEDFLGLNERFKEAYSKST
                     SISANAEEVFTVYASSYTSESLLNLRLLFKKFSQAKKETNKYADSIVKSIDEVYDILD
                     SIDLHSKNINQNLLTLKFLLANLKITGIESQSVELTEEKDELFIEFNRLVNKSKLAEL
                     ELAKSLHGNMKLLREGVDRVKKNMRNANQQIGIAIDIINESIQIFSEKQQDLSLNIPK
                     LQENNAKLRDSIDSIITNLQYHDIIRQKIEHVQVSHKEILNNLSSEDESEQKNYLNQI
                     GELVNIQSALLVRANKEYQRAIEQIIDKFKIIGSIAQSILNQCKDLKRIQESDLEQPF
                     TSVAHKLTNTTLPVSKFLSLLARVNNLLQSIKHNVNLMVETSAIDDEANRFGELLLKR
                     IKQPELMLQSNLIQQIETVTFDIITSRNQIGTLYMHLESKITDIERLSELISENTKSQ
                     EWESIPTTLKEIASNLLECDDKVSVLLREKLQASNDLGEIIHRAVNQVKYYEVFELKI
                     LEVIKLLNHIYSTLTGKEESQFDRDDLEFMRRLYTMESEHRIHNMVIKGNAGFDDLSE
                     TDQDESNIEFF"
     gene            complement(6102..6479)
                     /locus_tag="PLW22_02075"
     CDS             complement(6102..6479)
                     /locus_tag="PLW22_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU73520.1"
                     /translation="MDFKGYVLVIDDSVTNQVLVEALLGEEGIRVVTVSNAADAYRLI
                     DKEPPSLILLDLLMPNVTGIDFLKTIKQSERVNTIPVFIVTAANTELYRNQCEVLGVQ
                     EFFPKPVDIPLLVKRVKEVLAGV"
     gene            6625..7323
                     /locus_tag="PLW22_02080"
     CDS             6625..7323
                     /locus_tag="PLW22_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU73521.1"
                     /translation="MADVITISFEKTFEKCKLPVNQLLTAEQQAIIMDSINIVNYNRK
                     DIIVKQFSASSHVYYIIKGIIKVSSEQRKGKNLVVKIDTAGSFVGLISLLGGDSYNFT
                     VTAAENCTLAQIPISTLKSVMLQNTRFALEISQLISRHSLFLVNRLAGLLYKQLPGRV
                     ADLILYFADDIFCNKEFEFPVNRTELAELCGTTKESLIRTLSEFNHDRIIELQRNRIR
                     IISYDILKTLSRIG"
     gene            7343..>7704
                     /locus_tag="PLW22_02085"
     CDS             7343..>7704
                     /locus_tag="PLW22_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU73522.1"
                     /translation="MPKILIVDDIFVNRLLLKEVMKNLHVTCLEAQNGKEAIDIIIKE
                     KVDLVFMDIEMPVMNGLETTKYVREKLPSPKKYTPIVALTAHNPANFFEDFRDAGFDF
                     LMTKPYSIEKIKQAIEKVL"
     assembly_gap    7705..7804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7805..8673
                     /locus_tag="PLW22_02090"
     CDS             <7805..8673
                     /locus_tag="PLW22_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU73523.1"
                     /translation="SEESSSQSKPTETENGKNIKVTTTNTPFETDANTNDKKQTINDS
                     ILNDEIVHEENAITKHYTEVSPGVERVNTNALDSIIVEKSLTENSNQIEKDLEQSNMK
                     GASKKKPSSILRVIVFIAVSLFVILTFAFILRELAFAPDEADWESIKNKPEKVEPIKL
                     PSDYASKSDDFDKKFEASNPDDEESKTATGNAQPTQTTEEVIEKSLTQNARADNPSTI
                     YSLVVGSFAEKANAQNFVNNLKAKGFSAEVYSKPNGKYLAVIGRFSSKDEANKQKDKL
                     KSQFQGIWIISR"
     gene            8676..9383
                     /locus_tag="PLW22_02095"
     CDS             8676..9383
                     /locus_tag="PLW22_02095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOU73524.1"
                     /translation="MNRTKQCTYPHIGTVTYTYRTRCKRITLRVKKDNSIHVTIPYLV
                     KFSYAEDFVISKADWIQKKLKENAERAKPITEFKSRYHLVRLQAESGLSKCTVIKNGT
                     DVLLLYPSTADPASYSIQSLAKKIIAEVLRAEAKYYLPQKLNELATRHNFTYGKVTIR
                     NAKSRWGSCSGKNNISLSLRLMYLPEHLIDYVLLHELCHTKEKNHGPKFWQMLDSVCQ
                     GNSKMFSKELKKFPIPF"
     gene            complement(9413..9694)
                     /locus_tag="PLW22_02100"
     CDS             complement(9413..9694)
                     /locus_tag="PLW22_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73525.1"
                     /translation="MASVKDLKKDIKQMVKHLLDECYTQLTYSEPISKERILDIISDI
                     MVLEQETISKISKKTYKRGESTKVDYQKIANDFYDEVVELAERINSLDE"
     gene            complement(9808..10272)
                     /locus_tag="PLW22_02105"
     CDS             complement(9808..10272)
                     /locus_tag="PLW22_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOU73526.1"
                     /translation="MKSRTITQQAEIEQVINSVDVCHIGMVNLEGNPYVLPFNFGYEE
                     GVLYFHSGPEGKKIDIWKHNPSVCVAFSSDYHLRYQHEDVACSWSMKYRSVLIYGKVE
                     EITSLDEKRKCMNIIMKKYSGRDNFEYSMPAIKNVKVFRVIPERIEGKAYGY"
     gene            complement(10344..13160)
                     /locus_tag="PLW22_02110"
     CDS             complement(10344..13160)
                     /locus_tag="PLW22_02110"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOU73527.1"
                     /translation="MSSYKGLTDSEVIQSRNLHGVNIITPPPQTPWWKLLLEKFEDPI
                     IKILIVAAIIAIAIGITNGHYLEGVGIIVAILLATVMAFINEYKASKEFELLNSVNDE
                     LPVKVIRNGGVTSVPKRDVVVGDIVIVDRGDEIPSDGVLLESVSLIVNESSLTGEPST
                     NKTSIPTDSFKTTYSPNQVLKGTTVTDGHGIYRTTAVGDSTEIGKTARAATVEVNNPT
                     PLTKQLQKLSEYISSAAFFIAILAFVILTVFDLINPANNYTLEQEYILAVTVIAIIIL
                     LAKYWVPSVNAILSWIKLSAKIHLPYPNNTRRQMLVLLASAILLVLLGLVIAVLLNID
                     IFNASVVLDSNLLSHLLSYFMVSMTLIVVAVPEGLSMSVTLSLAYSMRKMTAENNLVR
                     KLQATETMGAATVICTDKTGTLTQNRMQVIEWDFQGDTGSNELPILVKLSLAVNSTAH
                     IDFSHEPYTIVGNPTDGALLLWLYQNGVDYRVLRDGFTTLAQHPFSSEKKYMLTLGQS
                     TDFVEKLLLVKGAPEKVLSMCDPQSVSSSIMEKLREFQVKALRTIAFAYSKSEVDSIT
                     DSDTPLGLTFLGVVGIADPLRDDVPTAISECHMAGIDVKMVTGDNQDTAIAIGKNAGL
                     LASNAEHASISGPDFEKLPDDEALKVLPGIKVMYRARPTDKLRMVSLLQRLHNVVAVT
                     GDGTNDAPALNQADVGLAMGSGTQVAKDASDIILLDDSFSSIVNATRWGRALYQNIQK
                     FLYFQLTINLVAVAIVLTGPFTGIDLPLTVTQMLWVNLIMDTFAALALATEPPHSEVM
                     KKKPRSPHEFILSSSIFRYLITTAVLFYLVLVGLLFYLKHNEGFSDRNLTGFFNVFVL
                     MQFWNLFNARGLFGGVGVFSFSSNKLFIAIAIIILLGQFAIVQFGGEAFRTVPLTGKE
                     WLIYIIATMPVLIFGYLAKKFIKI"
     gene            13272..13988
                     /locus_tag="PLW22_02115"
     CDS             13272..13988
                     /locus_tag="PLW22_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU73528.1"
                     /translation="MGRAFEYRKARKLKRWGNMARTFTRLGKEITMAAKAGGPDPETN
                     PKLRLLIQNAKAENMPKENVERAIKRATDKDTKDYKEVVYEGYGPHGIAILVETATDN
                     PTRTVANVRSYFNKYGGSLGTTGMLDFIFERKCSFKVAKNPNISMEDLELELIDFGVE
                     EVFAEDETIMLFAEFTQFGPIQKFLEEKGLSIISFQFERIPNDTKKLTPEQQTEVEKL
                     IEKIEEDEDVTNVFHNMAIE"
     gene            complement(14036..15139)
                     /locus_tag="PLW22_02120"
     CDS             complement(14036..15139)
                     /locus_tag="PLW22_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU73529.1"
                     /translation="MEDKLIVIATESNSKALVLKSYLEANGVECFLRSVNVLQGAIAE
                     GVMVLIRESDAEKALKLLSAMHHDEEIKKADQQLRKILVPVDFSVPSHNAARYAVMLA
                     SKYKAQIKLLHVFNSPVVDMIPFTDVSSIQIDFDMNYHVLYNSAKVRLVKFHENILEF
                     ANQMGFGEVQIGYTLREGYAAYGIIEASKRYKPGIIVMGTKGEGYRSTELVGSVAAEV
                     SDETHIPLLVIPEKAVLKGVDEVKRVLYITNFDSSDNASIRKLLKIVSPFQVELHCIH
                     VTGKSETPGINALITSTREYLASVTSNVSVKCDIIVGKETDKSVLEYIKKNNINLVAL
                     TSVKRNLIYKLFNPSLAKRMIYQSDVPVLLFHA"
     gene            complement(15247..16332)
                     /locus_tag="PLW22_02125"
     CDS             complement(15247..16332)
                     /locus_tag="PLW22_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73530.1"
                     /translation="MKMKRFTLFALFLAGMVSFVACEKDDTKDLNKDEAQQQITNAEQ
                     ELLTKSGEIVATDGYKVQEYFATQLPSPFYVKSDVSKSVVPSRFQKAYELTRQNLLLE
                     NPEIEFHFDYFLIEFFNDVKGTWTWAPDGWTHESTPTDKVIVKFPYPATNATNNVTIT
                     YYDFTTTLVYGETVPTGIKVKIEKDGNQVFSMVYSATIANMMKYSTKVTVTFGPFEIS
                     NEESFDASVNNKVLYSKNFTFKKDGKLFYSEDADLTITVVSEQSADILITAKQVIGNL
                     EFRLKIDGNGNELETGNPNDFASLSVYTTGGDKVGDFVFVEEEGYWVLYFKFTNGEQV
                     KAETLMPLVSERFYSFIDDLFSNLIFK"
     gene            16550..18286
                     /locus_tag="PLW22_02130"
     CDS             16550..18286
                     /locus_tag="PLW22_02130"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOU73531.1"
                     /translation="MKQFLSYILLSLNVIAWADANACTNYLVTRGASADGSTMVSYAA
                     DSHIRYGELYFKPRGIWPAGTMITLYDRGINKPLGQIPQAPETYQVIGFMNEYQVAIG
                     ETTFGGIEELMDSTAIVDYGSLMFLALQRAKTAREAIKVMVELVDQYGYASSGESFSI
                     GDPNEVWILEMIAKKTNLKYDKKTKQNVNIDKGAVWVAIRIPDGYISAHANQARIQQF
                     PLENHKTSISSKNLNLIFNPEVETVYSHDVISYARNHGYFNGSDSEFSFSDVYNPITF
                     DGARYCDARVWAFFNHASDGMGKYWDYAKGENLKNRMPLYVKPNRKLTPRDLMTFKRD
                     HLEGTELDMSKDAGAGPHGLPYRWRPLTWNVDGVTYFHERVTATQQTGFSFIAQMRSW
                     LPNPIGGIFWFGVDDAASTVYVPIYCGISRVPESYSEGNGDMLTYSPTSAFWVFNRVA
                     HFAYLRYDLIMKDVKKLQDELENKFEAYTPAIDAAALKLWENNTNLAIEFLTDYSVNT
                     ANSTVQKWNDLSNWLLVKYIDGNVKKEIDGKFLRNPWGYPVSPAQPGYREEWLRTIIN
                     ETGNKFKVPNQN"
     gene            18310..20964
                     /locus_tag="PLW22_02135"
     CDS             18310..20964
                     /locus_tag="PLW22_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOU73532.1"
                     /translation="MKKFYTLLLLALSGLLVIANPVDVKLAKKVAINYLSAKKGASID
                     TFDLKLVNTHQYEGKDALYIFAMSKGGFIIVSSDDEAKPIIGWSITNQMPKKIDNPVV
                     LERFNWYAKQVNHAAKSKIGDKSVKQEWQDILDGKIAKGVKAAGPLLTTIWNQEPYYN
                     MLCPTGTPTGCVATAMSQIMRYHQWPQSGKGWHKYEHPTYGTQYANFESTTYAWASMP
                     NELTSASTYDEKVAVATICYHAGVSVNMNYATDGSGAFSRDVLYALTNYFNYDPTTIQ
                     IYTFNPDNQTEWINMVKTEIDAGRPIYYSGSSSASGGHAWVCDGYNDNNELHINWGWG
                     GYANGYYAATAMIPSGTSYDFSESNDMIIGIQPNQSEFKHLWVRQASGFGTSSRGIQY
                     ISAVSNRVAWAVAFDGSGGNAKVKEYTMTTDGGATWKAGSINPANSAGFAASMICAID
                     DKTAWVPLYDGTNGGGMIAKTTDGGKTWTQQTSATFTKPNGFPNVVHFWDANNGFCMG
                     DPNGGYFEIYTTTNGGSNWTRVSQANIPAPLNGEYGVVGYYDVIGDNVWFATNKGRIY
                     KSTNKGASWQVYQTPLTSTAFEIAFKNSNEGVIYGWENNIDKYYRTTDGGTTWTEFTP
                     SGSVYTADLCWIPGTDTLISTGSNYSANKMGVSYSVDGGNTFTDYAPFYKNFQFTNMG
                     ASPNGTVWAGSFNSSANYGGMWKKGASVLSANFTVNKTTAYRNDSTVVFTDMTYGSPE
                     SWEWNFGEGAEPAIKTGKGPYTVKYTTYGDKTVTLTVQKGNDIHVYVKEKVLNVTWPS
                     GVETNNSDNKLTVYPNPTNSSVFVNIAGFTKGIIDVYNITGALVWTSGTETTEGKIDV
                     SNLSTGVYLIKIKGDNGTVATRKLTVTR"
     gene            complement(21024..21566)
                     /locus_tag="PLW22_02140"
     CDS             complement(21024..21566)
                     /locus_tag="PLW22_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HOU73533.1"
                     /translation="MKALIVVDVQNDFCPGGALAVTDGDKVVDPINELSKQFEADGLP
                     IIFTRDWHPANHLSFKENGGVWPPHCIAGTPGAAFHPNLYFPSVAFLVSKATEPDMEA
                     YSGFQGTGLASWLRQIDVDELVVAGLATDYCVKNTVTDAIKLGFKVTLALKAIKAVNV
                     NPTDGETAINEMKSLGVQIV"
     gene            complement(21572..22789)
                     /locus_tag="PLW22_02145"
     CDS             complement(21572..22789)
                     /locus_tag="PLW22_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73534.1"
                     /translation="MKKRLNTTIFDLPVQELRRGYRSDVYFWREKVVLENHDLHPRVT
                     MQVFQKNNAILCGIDEAIAVLKVASGRYSDYSKAYQLFDKLIDLKKLARKQFLTDKNR
                     YLQIEEERIKVSQELDNLWIDGFSEIEIDALWDGDSISPYESVMHIHGDASLFAHLET
                     IYLGILARRTKIATNVRRVVEAANGKTVLYFPARFDHWAVQGGDGYAAHIGGASGVST
                     DAQGEWWGAGASGTIPHALIAAVGGNTVKAVQFFHDTYPNATLIALVDFDNDCVNTAL
                     ECARAMGDKLWGVRLDTSENLVDKSVFPLMGTYKPTGVNPKLVELVRSALDREGFSNV
                     KIVVSGGFNPDRIRQFEGMGVPVDAYGVGSYLMQGSYDFTADIVHVNGKPMAKVGRYF
                     MPNPRLQRYNPNE"
     gene            complement(22856..23302)
                     /locus_tag="PLW22_02150"
     CDS             complement(22856..23302)
                     /locus_tag="PLW22_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOU73535.1"
                     /translation="MRTVFVFTALVLFSFALRAQEVSVYSVKNGKGDLITVSADTLNM
                     GEIYLDDLSSEHGVLLINLTNSGKKPLLIRNVTGCCGTNIKTWSKAPVLPGKQSEIRV
                     EFRIEPKPQRISRTVTLESNALGKPIIKLHIVGLVMQRRQANELVL"
     gene            23427..24080
                     /locus_tag="PLW22_02155"
     CDS             23427..24080
                     /locus_tag="PLW22_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU73536.1"
                     /translation="MGIDLKSFNGFIFDMDGVIVDNYLYHIDAWGEFCKRHGLNFEVE
                     DFTRKYFGKNNTDILQALFGKSLSSTEIDMLGEEKEAIYRELYQPYIKPLDGLVDFMK
                     ELRIQGGKIAIASSAPQSNINFVVEKICINEYIDVSVNGNMVRLGKPNPDIFLKAAEL
                     LSIQPGKCLAFEDSFSGIQAAINAGMQVVALATTHKKEEHDPTITIISNFKELLQGY"
     gene            24119..26092
                     /locus_tag="PLW22_02160"
     CDS             24119..26092
                     /locus_tag="PLW22_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOU73537.1"
                     /translation="MRFLAFIAILLAYSTITAQEKEIDLYSLSLEDLINIEVSISTKT
                     KVGLRETPGIVTIITKEEIKRSGARDIIELFHLFVPGFDFGVDVEGVVGMGIRGIWAH
                     EGKYLFMVNGFTMNDGMFACVPFGNHFALDNVEKIEIIRGPGSAIYGGFAGLGVVNII
                     TRDTYELGGKVTYTATHTGKQFSHNQLSVGQTIHDKDLLMNISATYGAGSRSDRAYTD
                     YYRNSRNLLKASDTYTKQLALKLDYKGFTIQTFLDDYLYYQIDLWDELYRGTPVHESF
                     KSNFVDLSYKFQRANLNVIPHITYEWQKPWQVNIPEINYSNNKIFQSLTPGISTYYSK
                     KTIQFVNGIEFKTDRLRLPSMVDTLYEETFKNGKDLLSYTNFSAYSQLLWNSKYVNLN
                     IGARYDYSSEFGDALTPRIGLTKLFKRYHFKLMGSKSFRTPGGILPNRIPNDYPKLYP
                     ESGITFEFENGFLISNNSMLTLNLYDITFEDIIIYGKDAQSGVGYYKNQGKIGTRGVE
                     MGLKMNTNKVLSTINLAYYSRKNSDSDSLFMVPDKTNQYLALSPLRINGLISYKISNR
                     TSFSVTASYFGERYSYNYIANNNDILVKQKTNLLVGMNMIVSDFPFKNFETQLIATNL
                     LGSDFYYLQPYKGAHGPLPGLDRSFGFRVIYEY"
     gene            complement(26142..26573)
                     /locus_tag="PLW22_02165"
     CDS             complement(26142..26573)
                     /locus_tag="PLW22_02165"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOU73538.1"
                     /translation="MEVKLKELLAISGYSGLFRFISQGRQGVIVESLTDGKRTLIPAS
                     ARVSAIADIAVFTQTEEIPLKDVLKKIEVLENGGPAINNKSADKEIRSYFEKVLPEFD
                     RDRVYTSDIKKIIGWYNLLQSKGLLEVLNQTDEEVKPEGEK"
     gene            complement(26624..26920)
                     /locus_tag="PLW22_02170"
     CDS             complement(26624..26920)
                     /locus_tag="PLW22_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop repeat-containing protein"
                     /protein_id="HOU73539.1"
                     /translation="MNTADFIEAVGWFGNIILSIGVLPQVVQTWRTHDVQSFNWLFLL
                     MWAFGVLFTFVYIAYNDMVVGKYQWPLWLNYLVNIIATFYLVWAKYYYGRLKKS"
     gene            complement(26933..27649)
                     /locus_tag="PLW22_02175"
     CDS             complement(26933..27649)
                     /locus_tag="PLW22_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOU73540.1"
                     /translation="MGKYGKWITGGLGWALFGPIGAILGFAIGSIFDSMETTKIYTGE
                     TSRNGFIVSLLVLVSAVMKADGKVLKSELEYVKSYFYTNFGPAAAREAILLLRDILKQ
                     AVPLRDVCLQIKENVDYHSRLQLLHFLFGIAQADGVVSSEELKVIIEIAGYLDISDAD
                     FSSIKAMFIPDTDKYFKILEVSPDATNEEIKKAYRQMAIKYHPDKVQHLGEDIRQAAE
                     QKFKMVNEAYEKIKKERGFN"
     gene            complement(27642..28238)
                     /gene="coaE"
                     /locus_tag="PLW22_02180"
     CDS             complement(27642..28238)
                     /gene="coaE"
                     /locus_tag="PLW22_02180"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOU73541.1"
                     /translation="MLLRVGVTGGIGSGKTLVCKILEVLGYPVFYSDLVAKSITDTDP
                     EVMEKVKGLFGNNIYPNGKLNRKKVAELVFANDTLLKELNSIIHPAVARSFEQWCLQN
                     AKSGLVFKESAILFETGIFRELHKTILVTAPEELRIKRVVERDGVTELEVRARMAKQM
                     PEEQKKKLADFIVDNSSNQLVLPQVLGIVQILTSIKNG"
     gene            complement(28238..29170)
                     /locus_tag="PLW22_02185"
     CDS             complement(28238..29170)
                     /locus_tag="PLW22_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOU73542.1"
                     /translation="MNKRVYMFLFFLVISIILWLLIKLRYEYVSTITYKVNLSELPET
                     KVALTSDSLTVTLRVKALGYNLLRYKLFKYYKPLSVSAQGADFVKGNCYVVLSGQLDN
                     FTSQLGTDFTLLEVSPDTLYFHLSDVVQKRVPVKPDLNITYSKQHMQAGSYTVVPKVV
                     KVTGPASIIDTLSCVYTQPLTLEDIYDNINTTIPIKPIKGLSFKPSEVSISIPVEKFT
                     EARVNVPISCINTPYGYEVMLSPKTVSVTCNVAVSKYFALKPDHFSIICDYNDLVMES
                     SGKAIVKLVRYPDFIGNIQIDPRKVDFVIVKKKQ"
     gene            complement(29381..29641)
                     /gene="yajC"
                     /locus_tag="PLW22_02190"
     CDS             complement(29381..29641)
                     /gene="yajC"
                     /locus_tag="PLW22_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOU73543.1"
                     /translation="MTFLMFGLIIVVFYFFMIRPQMKRQKELRKFQESLKKGDKVIIA
                     GGVYGKVTEVKDEYVLVEIADNVEVKVAKSTIIRDVTDMQTR"
     gene            complement(29768..30181)
                     /locus_tag="PLW22_02195"
     CDS             complement(29768..30181)
                     /locus_tag="PLW22_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOU73544.1"
                     /translation="MRITSLLFGFTALFAISCGSNNNQGSTQETDSTQLAELTYVEDF
                     FDVGTIHSGEIVRHSFRFRNTGNAPLVVKDIIPSCGCTKVDVTSRILKPGEEASVEVV
                     FDSKGWFGSQYKSVTLRTNGVIREKSVTLKANVVP"
     gene            complement(30168..31166)
                     /gene="nusB"
                     /locus_tag="PLW22_02200"
     CDS             complement(30168..31166)
                     /gene="nusB"
                     /locus_tag="PLW22_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOU73545.1"
                     /translation="MINRRLIRIKALQVLFAFFRNEGDSLSALERELFHSIEKSYHLY
                     LLLLLLPENMVEHAQAKIELGKQKFRPSPEELNPNLRFVQNRAVAALAACKELQAKAN
                     DNRLNWNHFPEVTKSLFNHFVASDFFSDYMNAEHNSFADDKAVLVNLLSKILNANDDF
                     EAFLEEQSIYWNDDLEFVSSMAAKTIKKIDENTASVPVMSLFSNADDEEFAKRLLRKT
                     VLRHKENLELIDKFTVNWELDRIAAMDIAIMEMAITEILEFPTIPVKVSMNEYIDIAK
                     FYSTEQSNSFVNGVLDKVIAHLKENGQIQKQGRGLIGENDESLSPENINSFEDEDN"
     gene            31366..31737
                     /locus_tag="PLW22_02205"
     CDS             31366..31737
                     /locus_tag="PLW22_02205"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOU73546.1"
                     /translation="MIEEFDNTNDLDKNTREEVFSQVVKAGKRTYFFDVKATRGNEYY
                     LTITESKKRMGKDGKMYYEKHKIFLYKEDFEKFIESLNSVFDYIKANQEIVPRTDHTD
                     EMELSEVESDYSSVDFDDLGK"
     gene            31899..34400
                     /gene="feoB"
                     /locus_tag="PLW22_02210"
     CDS             31899..34400
                     /gene="feoB"
                     /locus_tag="PLW22_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOU73547.1"
                     /translation="MLLSELQNGEYGIIAKVKGRGAFRKRITEMGFVKGKKVTVVKNA
                     PLKDPIEYSIMGYEVSLRRSEASLIEVITPSEAKQILNYHPDNHQLVTHTEPEIIDTG
                     VKELSKTINIALVGNPNAGKTSIFNHLAGAREHVGNYSGVTVDSKNAKFKYKGYTINI
                     TDLPGTYSLTAYTPEEVFVRRFIHEQNPDIVINVIDSSNLERNLYLTTQLIDMDIRVI
                     VALNMYDELLEKGDRFDYTMMGKLLGIPFVPTVGSKGKGLDRLLDTTINVYAEKDKVT
                     RHIHVNYGEEIEKSILSIQNEIKKNTAITINYSSRYLAIKLLEKDKVADKVLRNEPNY
                     QEIRNQTDREVSRLESLFHEDTETLITDAKYGFIAGALKETFKESSHKKRLVTEQIDA
                     ILTHRLLGFPIFIFLIWLTFYTTFTLGQYPMEWIELGVEWISNTISTYMASGPLKDLL
                     VNGIISGVGSVIVFLPNILILFLFISLLEDTGYMARAAFIMDKLMHKIGLHGKSFIPL
                     VMGFGCNVPAIMASRTIENRNNRLVTMLITPFMSCSARLPVYILILGAFFPENAGNLL
                     FVIYFTGILLAIGTALAFKKTIFKAEDLPFVMELPPYRMTTLRSTLLHMWHKGSQYLK
                     KMGGVILVAVIFVWALGYYPRKVNYTRDYNAEIETITQKISEEKLSQPSMDSLNQTIH
                     QLELEMEAERQANSYLGRIGKFIEPAIRPLGFDWKMGVSLLSGVAAKEIVVSTLGVLY
                     QTDPDSDSGGLAQKLRNETHQSGKLSGQVVFTKVTALAFLIFILVYFPCVAVISAIKN
                     ESGSWKWALFTIVFTTSLAWVLAFLVYQIGNIIIG"
     gene            34616..35077
                     /locus_tag="PLW22_02215"
     CDS             34616..35077
                     /locus_tag="PLW22_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73548.1"
                     /translation="MKKVTQKIVSITIATIVLLSTSGFNLFIHHCQCEGEIHLSIFNE
                     VGCNHTHAKTHAHCCNVHFDETLKFNEKQSCGCWNEVLKVKTDSYSNIQKVETGNLFS
                     KYIAVNHHKGLNTTIHKLIPTYKTNWDKAPPCSTGKKICIENHQLKIAHTI"
     gene            35178..>37261
                     /locus_tag="PLW22_02220"
     CDS             35178..>37261
                     /locus_tag="PLW22_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU73549.1"
                     /translation="MKRILTLLLLWFSAQHIIKAQSKDFVYGYVYELNEKGDSIPLQG
                     AAVIWLNSQVGTTTDESGYFSLNKVQGNQIAISFVGFKTDTISIENVDFPLRHVLKST
                     ISIDAVTVKADRANTFIQQLNPIQTVVLTSGELTRAACCNLSESFETNASVDVSYSDA
                     ITGAKQIQLLGLAGIYTQMQYENIPTMRGLASTFGLSYVPGPWMESIQVSKGTASVSN
                     GYESITGQINIEYKKPWAEEKSYLNLYTNSLGMAELNANYAFDVTPTVSSMVLVHGSM
                     LSNRVDHNHDQFLDHPLTQQYHLFNRWKYQGKKWESQVGVKFMNEDRLAGQVKYYTNT
                     QPLIGNPYGVKYSTQLLEAFNKTGYISQRPNTSIGLINAVSLHSQDATIGVRMHNAKQ
                     LSYYGNLVAITYIGNTNHNIKGGATLRYDRYDETFNNTPYDRTEWVKGIYGEYTYKHL
                     EILTLMAGIRVDQNSRFGTLVTPRFHGMVKPVSTLTIRFSAGKGYRSPNFIAENSNLL
                     VSSRNLVVEENPRIEEAWNAGISMNQKFKLLNQNYSLTADYYYVWFENQFIADIDRDN
                     IAVYFYNLKGKSYSNTLQLELSGEPIRNLQFTLAYRINDVKQTINDTLKQKPLVSRNK
                     GLLTAGYKTPSRKWQFDYTIQYNGSGRLPKGYGLNRESYYNPFITMNIQVTKMFRKFD
                     FYIGVENLTGFT"
BASE COUNT        11547 a         7616 c         7269 g        10729 t
ORIGIN      
        1 ttgatgtaaa ggggttaata atctcattgg tgatgtttga aataaagtgc gattttaggg
       61 cttcggattc ctccaacttc ttgtttacct gcttcaactc agcattcaga acttttagct
      121 catcgagagc tttcttgtta agttcaaacc tgcgctggag ttcagcaagc agttcgttat
      181 cggttaattc ttcaggtttt ttagccatag tagtaagctt tatactttcc ggtaaatagt
      241 aggttttagc tgtttaagcg ggacatccat gtttaaaatc gattcggaat gccccaaaat
      301 taggtagcca ttgggtttaa tacgttggca cagtttatta ataacggctt cctgtgtgtt
      361 cctatcaaag taaataagta cgtttcggca aaaaacgaca tcgaacaagt ccttggtatc
      421 gtagctggaa tccatgaggt ttaagcgagc aaaggttacc ttggagcgaa cgtgaggggc
      481 aattttgatg gttgggtttg ctctatcctt gctcctaagc atgtatttcc gcttaagggt
      541 tagtggaata gccccaactt tttcctcctt gtatatggca tcaattgctg tttgaagaat
      601 tcgggtagag atatcggtag caaaaatgga aaaattaaag ccagggtgtg tttcagcaaa
      661 ctcgaaaagc accatggcga gggtgtaggg ttcttcaccg cttgaacagc ctgcactcca
      721 aaccttaaaa atactcgact tatttgcccc gtaatacgcc ggaagtaatt cggttgttaa
      781 aaactcgaaa tgggcaggct cacggaaaaa atcagttttg ttggtgctaa ccacatccaa
      841 catatgaata atctcgctat caaagccttc gcggctgaaa agatagtcga agtaagcctt
      901 aaaagaggca atgtttaaat gttttaacct tttctggagg cggcattgca gcattacctt
      961 ttttataggt ggaagtttaa tgccgctctc cttgtagatt aatgcactaa gccgtttaaa
     1021 ctcatcgtcg gtaagctgta gcttgtagta atccacaaac tcattagcct gcataaaata
     1081 ttcaattact gttgtgcatc ggttccctcg gcaactgact ggttggaatc aacaatcgag
     1141 gtgatttcat ctactgagaa gatggcatcc atgttaagta tgatgataaa acgttcctca
     1201 acctttgcca tgccaattat aaactcagat ttataacggc tgccaagggt gggcaatggc
     1261 attatctgac tattatcaat ctccagaacc gcctgcacgg aatcgacaat ggcaccaaca
     1321 tgcaccgatt cgccatcaac gtcaatatcc agaactatga tacaggtgtt attggtgtac
     1381 tcggtttcag gcattccaaa cttgatacgt gtatcaatta caggcaatac agccccccta
     1441 aggttaataa cacctttcag gtaatcaggt gccttaggaa cctttgttat tggagtcatc
     1501 tccaaaatat tcaacacctt gctgacatgt gcagcaaact cctcgttacc tagcttgaag
     1561 gtaagatatg atttaatttg atttttgttc tcggtatcca ttgtaaaaca tttttggttg
     1621 ttacttactt gttgcaaaca ttttgataat cttattggtg tccattacaa gggcaactgt
     1681 tccgtcgcct agtattgtcg cacctgagaa aatatcgtgg ctcttgtagt gtttccctaa
     1741 aggtttgagt actgcctggt attcaccaat tacatagtca accactaggc caaccctgtt
     1801 atcctcaaag tttactacta caacctgttc tagttcctcg ctacttggct gcaggttaaa
     1861 ctccttccgt aagctaaaga atgggatttg agttccatcg agggttacaa gattgttgaa
     1921 agagctgtag atactttcct tgtccaccgc ataaatctta tcaattgaag aaagagggat
     1981 aacaaagggg gaactatcaa tactaaccag caaaccatcg ataattgaaa gggttaaggg
     2041 cagctttatg gtaatagttg ttcccttgcc aacttccgat tcaacctcaa cctcgcctct
     2101 gatatcggaa attttctttt taaccacatc catacccact cccctgcccg acacatcggt
     2161 tacctttttg gccgtagaga atccgggcaa aaagataagg tcaagaatct ccttcttgct
     2221 catcttttta tcggaaggta ctaaaccctt tgagattgcc ttggcatgta tgatctcagg
     2281 atcaatacca gcaccatcat cgctaacctg gattattaca cttgccccgg agtagaatgc
     2341 tttcaggtga atttttccct ttgccggctt tccgtttgca atacgaaccc gagtatcctc
     2401 aattccatga tccatgctgt tcctgataat gtgcatcagg gggtcggaca ggttttcgat
     2461 gatggtctta tcaagctcag tatcggctcc ctcaacaatg aaatcgacct ccttgtgcag
     2521 ttcagccgat aaatccctga caagcctctg aaaacgacca aaaaggtttt caatgggtat
     2581 cagaacgata ctaaaggcaa tatcgcgaag ctggcgggtt agcttgtgaa cgttttcaac
     2641 tataggcgcc aaacctggca cattggtttg ctctgcaaat aagcttaaac gggcttgtgt
     2701 ggttactagc tctgacacaa ggttcattag gtggtcaagc ttgtcggacg acacgcgaat
     2761 gcttgcagcc gttttgtcct ttgtggctat tcgctcttta gtgaactgtt tggccttttc
     2821 cttattgatt cgagcagcag cttttgttgc caggcgttgt atggttacca atccaacatc
     2881 cttctgaacc tctgccaatt gcgatacttc ttcaacaaat aatttatcgg caagcaagtt
     2941 ttcctctgcc aacttttgta tttcaagctt tgaatcggat tcaacaaaga tgaacacatc
     3001 atgaatggca tttacatcct ggtcggttgt cagcaacact tcccagtagg tatagcaaag
     3061 gtttggggtg atttccttta ttccaggcag cctgttgaaa tgggcaaaaa ccttgtgctc
     3121 acctaatgag caaagctcat caagaaggta aagggggttt gttccgtttt taaaaatatt
     3181 ctcattgggt tcaaaaagaa tgtaataggt agatagtgat tccgattttt cgttacttga
     3241 tttagcagat tcggcttctg tttcagcatt actggggtta attattctat taattttccc
     3301 cattaagcca ttgtgaaccg ttttaaagtc gggatcctca tagttttgct catcgagcat
     3361 cgccttgagg tgatccaccg atgaaaaggt tatgttcaat atatccttgc ttacctcgag
     3421 ctgtccattg cgaatcatgt cgtaaatggt ctcaaggttg tgcgtaaagc tatcgataag
     3481 gtcaaaccca aacatggcgc tgttaccctt aagggtgtgc atcaccctaa agacctgttg
     3541 aatcagagcc tcatcgttag ggcttttttc aagttctaaa agcgacactt caagcttatc
     3601 aattagctcc gcagcttcct ctataaactt tttcctaaag ctatccatta cctaatcact
     3661 ttattgattg ccgaaatcag tttagctgga acaaacggtt taataatcca ccccgtagca
     3721 ccggcatctt tagcttccat tttttttgag gtttgcgatt cggtagtcag gaataggatt
     3781 ggaatgcgtt cgtattccgg cattgcccta atatgcttaa taagctctat gccatccatt
     3841 tcaggcatat gaagatcggt gatcaccaaa tcgattgttt taccgtcaag gtgttttaag
     3901 gcatctttgc catcaacacc aacaagcaca ttgtaccctt cgttctcaag ggtaaatcga
     3961 acaacttcgc gaatactatc ggaatcgtct acaattagta ttgtttttgc catagcaatg
     4021 gatgatttat tttgttaata ttctgatttt atgcctgatc gttcaagaat tgtgctctgg
     4081 tcagcagtta attcaaactt aactgtcgat tttatacctc gctctttcat ggagaagtta
     4141 aaagagcgta cgagttgtat aaagccaagg tcaattacct cagcgttttt aattagcaag
     4201 tcagtaggtt tagcctcatt aaatagctta atcagttcac tcttgatatc actgatatta
     4261 ttaaggctta aatcgccact cagaatgacc agcgatgaac ccttctgcct ttcgatggaa
     4321 aaattttttt cttgctttga catactcttg gtttgtttaa aagaactcaa tgtttgactc
     4381 atcctgatcg gtttctgata agtcatcgaa acccgcattc cctttaatta ccatgttgtg
     4441 tatcctatgt tccgattcca tggtataaag tcttcgcata aactccaaat catctctatc
     4501 aaactggctc tcctccttgc cagttaacgt ggaatatatg tggtttagta gttttattac
     4561 ttcaagaatt ttaagctcaa aaacttcata gtacttaacc tggtttacag ccctatggat
     4621 tatttcacct aaatcattac tagcctgaag cttttcccgg agtaaaacgc ttaccttatc
     4681 gtcacattca agcaggttgc tagcaatttc cttaagggtt gttggaattg actcccattc
     4741 ctgcgattta gtattttctg aaattaactc cgataatcgc tcaatatcag ttattttgga
     4801 ttcaaggtgc atgtacagcg taccaatttg gtttcgggag gtaattatgt caaatgttac
     4861 tgtttcaatc tgttgaatta gattcgactg aagcattagt tccggctgtt tgattctttt
     4921 caacaacaat tcaccaaatc ggttagcctc atcatcaatt gcagaggttt caaccattaa
     4981 gttaacgtta tgcttaattg attgaagcaa attgtttacc cttgccagta acgaaagaaa
     5041 tttgctaaca ggtaacgtag tattggtaag cttatgtgca acacttgtaa agggttgctc
     5101 caaatccgat tcctgtattc tttttaaatc cttgcactgg tttagtatgc tttgcgcaat
     5161 gcttccaata atcttgaact tgtcaattat ctgctcaatg gctctttgat actccttgtt
     5221 tgccctaaca agcagcgccg actgaatgtt aacaagttct cctatctgat ttaaatagtt
     5281 tttctgctca ctttcatctt cagaggatag attatttagt atttccttat gcgatacctg
     5341 tacgtgttca attttttggc gaataatatc gtggtactgg aggtttgtaa taatgctgtc
     5401 aattgaatcg cgaagttttg cgttattttc ttgcagtttt ggtatattga ggctaaggtc
     5461 ttgctgtttt tccgaaaaaa tttgaatgga ttcattaata atatcaatgg caatccctat
     5521 ttgttggttg gcatttcgca tgttcttttt aaccctatca acaccttcgc gcaaaagttt
     5581 catgttgcca tggagcgact tagcaagttc aagttcagct agctttgact tgtttactaa
     5641 cctgttaaac tcaataaaaa gttcatcctt ttcctcggta agttcaactg attgggactc
     5701 aatgcctgtt atcttaaggt tggctagtaa aaacttgagg gttaggagat tttggttaat
     5761 gtttttgcta tgtaggtcaa tggaatcgag aatatcataa acctcatcaa tgctttttac
     5821 aatggaatcg gcgtatttat tggtttcctt tttagcctga ctgaactttt taaaaaggag
     5881 cctgaggtta agcaacgatt cactagtata gctggaagca tatacggtaa acacctcttc
     5941 agcatttgct gaaattgagg ttgactttga gtatgcttcc ttgaaccttt cgttaagacc
     6001 taaaaaatcc tcagcggcgc aatttagtat ctcaactata agactatcaa ttacggcaaa
     6061 ctgttcagat atctccttga tttttttcat aacaatttat attacacccc agccaaaacc
     6121 tctttcaccc tcttaaccag taatggtata tcaactggtt tgggaaagaa ttcctgtact
     6181 cctagcactt cgcattgatt acggtaaagc tcagtattag cagcggttac tatgaaaact
     6241 ggaatggtat ttacgcgctc actctgcttg atggttttaa gaaaatcaat tccagtaaca
     6301 ttaggcataa gtaaatccaa gagaattagg cttggtggtt ctttatcaat cagcctatag
     6361 gcgtcagcgg cattgcttac tgttaccacc ctaattccct cttcgccaag cagagcttcc
     6421 acaagtacct gattcgtaac cgaatcgtca attactaaaa catagccttt aaaatccata
     6481 gtatttcaaa atcttttcaa atctataaaa acgaaaccac agaataacaa atcatatgat
     6541 gatataaatt accgcaacag gtgatttgta tcactttttt atttgataga aattatttat
     6601 ctttaagagt ctttaaatga aatcatggcc gatgtaataa ccatatcgtt tgaaaagaca
     6661 tttgaaaaat gcaaattgcc tgttaaccag cttctaacag cagaacagca ggctatcatt
     6721 atggatagta tcaatattgt aaactacaac cggaaggata taattgttaa acagttttca
     6781 gcatcaagcc atgtttacta tattattaaa ggtattatta aagtgagtag tgagcaaaga
     6841 aagggcaaga atttggttgt aaagattgat accgctggaa gttttgtagg cctaatttca
     6901 cttcttggcg gcgattccta taactttacg gttacagcgg ctgaaaattg cacattggca
     6961 caaatcccca tttctacgct gaaatcggta atgctacaaa atacacgatt tgcccttgaa
     7021 attagccagc taataagcag gcatagccta tttcttgtaa ataggttagc tggattactt
     7081 tacaagcaac tacccggccg agtagccgat ttgattcttt actttgccga tgacatcttc
     7141 tgcaacaaag agtttgaatt tcccgtaaac agaactgaac tagctgagct ttgcggaaca
     7201 actaaggaaa gcttgatcag aactttatcg gaatttaacc acgatagaat tattgaactg
     7261 caacgtaata gaatcaggat tattagctac gatatactaa aaacattgag cagaattggt
     7321 taatgtaata aaagtaaagt gtatgccaaa aattcttatt gtcgatgaca ttttcgttaa
     7381 caggcttctt ctaaaagaag tgatgaaaaa cttacatgtt acctgccttg aggctcaaaa
     7441 cggcaaggaa gctattgaca ttattattaa agagaaagtt gacctggttt tcatggacat
     7501 tgaaatgcct gtgatgaacg ggctggaaac caccaagtac gttcgtgaaa aactcccttc
     7561 gcccaaaaaa tacaccccca ttgtagcatt aactgcccat aatccggcaa acttttttga
     7621 ggatttcagg gatgcaggct ttgattttct gatgactaaa ccatactcca ttgaaaaaat
     7681 taaacaggct attgaaaagg ttctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnncatcgg aggaatcatc aagccaaagc aagcctaccg aaactgaaaa tggtaaaaat
     7861 attaaagtta ccactaccaa tacccccttt gagacagatg cgaatactaa tgacaaaaaa
     7921 caaaccatca atgattcaat tttaaatgat gagattgtac atgaagagaa cgctattacc
     7981 aaacattaca ctgaggttag cccgggtgtt gagcgcgtta ataccaatgc ccttgacagc
     8041 atcatagtag aaaaatcgtt aacagaaaac agtaatcaaa ttgaaaaaga tttggaacaa
     8101 tccaatatga aaggtgctag taaaaaaaag ccatcatcaa ttctacgagt tattgttttt
     8161 atagcagtat cgctgtttgt tatactaaca tttgcattca tattaaggga gttggccttt
     8221 gcccccgacg aagccgattg ggaaagcatt aaaaacaaac cggaaaaggt agagcccatt
     8281 aagttgccat ccgactatgc ttcaaaatcc gatgattttg ataagaaatt tgaagcctcg
     8341 aaccctgacg acgaggaaag caaaacggct acaggtaatg cgcaaccaac acaaacaacg
     8401 gaagaggtaa ttgaaaaatc gttaacccaa aacgctcgtg ccgacaaccc atcaacaatt
     8461 tacagtcttg tggttgggag ttttgccgaa aaggcaaatg cccaaaattt tgtgaacaac
     8521 cttaaagcaa aaggttttag cgctgaggtt tacagtaaac ccaatgggaa atatcttgca
     8581 gttatcggtc gatttagcag caaagatgaa gccaataaac aaaaggacaa gttaaaaagc
     8641 caatttcagg gaatatggat tattagccgt tagcaatgaa cagaacaaaa cagtgcacct
     8701 acccacatat tggaacagta acctacacgt atagaacccg atgcaaaaga atcactttaa
     8761 gagtaaaaaa agacaactca atacatgtta ctattcccta cctagtaaaa ttctcatatg
     8821 ccgaggattt tgtaatatca aaagctgatt ggatacaaaa gaaactcaaa gagaatgcag
     8881 agcgtgccaa gcctattact gaatttaaga gtaggtatca cttagttcgc ctgcaagctg
     8941 aaagcggcct gtccaaatgt acggttataa aaaatggaac cgatgtacta ttactttatc
     9001 cgtcaactgc agaccctgca agttacagta tacaatcact agctaaaaaa attatcgctg
     9061 aagtattacg cgctgaggct aagtactatt taccccaaaa actaaatgaa ttagccacta
     9121 ggcataattt cacctatggg aaggttacaa ttcgtaacgc gaaatcgcgc tggggaagct
     9181 gtagcggtaa aaacaatatc agccttagtc tcaggctaat gtatttacca gagcatctta
     9241 ttgactatgt actcctccat gaactttgtc acacgaagga aaagaatcat ggccctaaat
     9301 tttggcaaat gttggactcg gtttgccaag gaaattcaaa aatgttctca aaagagctta
     9361 agaaatttcc aatccctttc tagctttaac ctaataaccc acaaacaggg aattactcat
     9421 ccaaggagtt aatgcgttca gccagttcaa ccacttcgtc gtaaaaatca ttggctattt
     9481 tctggtaatc aactttggtt gattcgcccc ttttgtatgt tttttttgag atttttgaaa
     9541 tggtttcctg ttctaaaacc attatatcgg aaatgatgtc aagaatccgc tccttagata
     9601 ttggctcaga ataagttaat tgggtatagc actcgtcaag caaatgtttt accatttgct
     9661 tgatgtcctt tttaagatct tttacgctgg ccatatcaaa atattaacta caatttttcc
     9721 atataaatat aaaccatttc ttaaaaaact agtaataatt gatatttttt ttgatgaaaa
     9781 gtgttttcaa aattttactg gatttcacta gtatccgtaa gccttacctt caatcctctc
     9841 agggataact ctaaaaactt ttacattttt aatggctggc atactatact caaaattatc
     9901 gcggccagaa tattttttca ttataatatt catacatttc ctcttttcat ctaaactggt
     9961 tatttcctca accttcccgt agataagaac gctacggtac ttcattgacc atgagcaagc
    10021 aacatcctca tgctggtagc gtaaatggta gtcggaacta aaagctacac atacgcttgg
    10081 gttatgtttc caaatatcta ttttttttcc ttcggggcca gaatgaaagt aaagcacacc
    10141 ttcttcatac ccaaagttaa agggtaaaac gtaagggttt ccctccaaat tgaccatacc
    10201 aatgtggcaa acatcaaccg aattgataac ctgttcaatt tctgcttgtt gagtaatagt
    10261 ccgagatttc attgcaatta cttttattgt ttaatacaaa ttaagtaaaa aatgatttcg
    10321 cacgctactc actatgtacc gttttaaatt tttatgaact tttttgctag gtaaccaaaa
    10381 atcagcacag gcatggtagc aataatataa attagccatt ccttaccggt taatggaact
    10441 gttctaaaag cttcgccacc aaactgtact attgcaaatt gtcctaacag aattattatg
    10501 gcaatagcaa taaatagttt attggacgaa aagctaaaca caccaacacc accaaacaac
    10561 cctcgtgcgt taaaaaggtt ccagaactgc attaaaacaa atacattaaa gaagcctgtg
    10621 agattcctat cactaaaacc ttcattatgc tttaagtaaa atagtaagcc aaccagaact
    10681 aagtaaaata aaactgctgt agttattaga tacctaaaaa tactagaact caaaataaac
    10741 tcatgaggtg aacggggttt ctttttcata acctcagaat gtggtggttc agtggctagt
    10801 gcaagagctg caaaggtgtc cataatcagg ttaacccaca gcatctgggt aacagttaag
    10861 ggtaaatcga tcccagtaaa tggcccagtt agcacaattg caacagcaac aagatttatg
    10921 gtaagctgaa agtacaggaa tttctgaatg ttttggtaaa gagccctacc ccagcgggtt
    10981 gcatttacaa tgcttgagaa cgaatcgtcc aaaaggataa tatcggaggc atctttggcc
    11041 acctgggttc ctgaacccat tgctaaaccc acatcggctt ggttaagggc cggggcatca
    11101 ttagtaccat cacctgtaac ggcaacaaca ttatgtaacc tttgaagtag cgataccatc
    11161 cgcaacttgt cagtaggtcg agccctgtac atcactttta taccgggcaa cacctttaaa
    11221 gcctcatcgt cgggaagttt ttcaaaatcg gggcccgaaa tggatgcatg ttcagcattg
    11281 ctggccagta atcctgcatt tttaccaata gcaattgcag tatcctggtt atcgcctgta
    11341 accatcttaa catcgatacc agccatatgg cactcactta ttgcagtagg aacatcgtcg
    11401 cgcaatgggt cagcaatacc aacaactccc aggaatgtta agcccagagg cgtatcagaa
    11461 tcggtaatgc tatcaacttc actcttggaa taggcaaatg caatagttcg caatgccttt
    11521 acctgaaatt cccttagttt ttccattata ctactactaa ccgattgggg atcgcacatg
    11581 gacaaaacct tttcgggtgc gcctttaacc aaaagtaact tttccacaaa gtcagtagat
    11641 tgccccaggg ttaacatgta ctttttttcc gatgaaaaag gatgctgagc taaagttgta
    11701 aaaccatcgc gaagcacacg ataatcgaca ccattctggt aaagccaaag taaaagggca
    11761 ccatcggtag gatttccaac aatggtatat ggttcatgcg agaaatcgat atgagccgta
    11821 gaatttacag ccaacgataa ttttacaaga atcggaagtt cattgctacc cgtatcgccc
    11881 tgaaaatccc actcaattac ctgcatacgg ttctgggtaa gtgttccggt tttatcggta
    11941 catattaccg tggcagcccc catggtttca gttgcttgaa gttttcgtac caggttgttc
    12001 tcagcggtca ttttgcgcat gctgtaggca aggcttaggg taacgctcat tgagagacct
    12061 tcaggaacag caacaactat cagggtcatg gataccataa aatagctaag caggtgactt
    12121 aatagattac tatcgagcac caccgatgca ttaaaaatat cgatgtttaa taaaaccgct
    12181 attaccaatc ccagtaaaac cagtaaaatt gccgatgcta gtagcacaag catctgtcgt
    12241 ctcgtattat tggggtaagg tagatgaatt ttagctgaca actttatcca gctaagaatt
    12301 gcatttactg aaggaaccca gtacttggct agcaaaatga taatggcaat tactgtaaca
    12361 gccagaatat actcctgctc aagggtatag ttgttagctg gatttatcaa atcaaaaaca
    12421 gtgagtatta caaatgccag aatagctata aagaatgctg ctgaactaat gtactcgctc
    12481 agcttttgaa gctgcttggt taaaggggtt ggattattta cctctacggt ggcagctcgg
    12541 gctgttttac caatttcagt actatcgcct acggcagttg tacggtatat gccatgacca
    12601 tcggtaaccg tagtgccctt aagaacctgg ttgggtgaat atgttgtttt aaagctatca
    12661 gttggtattg aggttttgtt tgtagatggc tctccggtaa gggatgactc gttcactatt
    12721 agcgaaaccg attccaaaag aaccccatcc gagggaattt catcaccacg atcaacaata
    12781 acaatatcgc cgactaccac atcgcgctta ggaaccgatg taacccctcc attacgaata
    12841 accttaacag ggagttcatc gttcacactg ttaagcagtt caaactcttt tgatgctttg
    12901 tactcattta taaatgccat gaccgtagcc aatagaatag ccacaataat cccaacgccc
    12961 tccaagtaat ggccattagt tatgccaata gcaattgcaa taatggcggc tacaatgaga
    13021 atcttaataa taggatcttc aaacttttca agtaatagtt tccaccatgg agtttgaggt
    13081 ggcggggtaa tgatattcac accatgcaaa ttcctgcttt gaataacctc actatcggtt
    13141 aaacccttgt aactactcat atctgcaaaa aataagcaac acaaagaaaa caaaaagatt
    13201 taagaatggt tggtcgaata gccaaaaaaa tatacgtttg cagttaaatt gaactaacaa
    13261 cttttcacaa aatgggaaga gcatttgaat accgaaaagc acgcaaactg aagcgctggg
    13321 gaaacatggc taggacattt actcgtctgg gaaaagaaat aaccatggcc gcaaaagccg
    13381 gcggacccga tcctgaaact aaccctaaac tccggctttt aatccagaat gcaaaggccg
    13441 aaaacatgcc taaggaaaat gttgagcgtg ccataaaaag agcaaccgat aaagacacca
    13501 aggattacaa ggaagttgtt tacgagggtt atggccccca tggcatagcc attttggttg
    13561 aaacggcaac tgataaccct acaagaactg tggctaatgt tcgtagctac tttaacaagt
    13621 acggaggttc gcttggaact actggtatgc tcgatttcat ctttgaacga aagtgctcat
    13681 tcaaggttgc caaaaatcca aacatatcaa tggaggattt agaacttgaa ctcattgact
    13741 ttggagttga ggaggtattt gctgaggatg aaaccataat gctatttgct gagttcaccc
    13801 agtttgggcc tatacagaaa tttctcgaag aaaaaggatt aagcattatt agcttccagt
    13861 ttgagcgaat ccctaacgac accaaaaaac tcacgcccga acagcagaca gaggttgaaa
    13921 aactcattga aaaaattgaa gaagatgagg acgtaaccaa tgtgtttcac aatatggcta
    13981 ttgagtaaat gtccattaca attatcaaaa tggccaccct aaagtggcca tttttttatg
    14041 catgaaaaag gagtaccggt acatccgatt ggtaaatcat ccgtttagcc aacgatggat
    14101 taaagagttt ataaatcaga ttccttttaa ccgaagttag agcaacgagg ttaatattgt
    14161 tcttttttat gtattcaagg acactcttat ccgtttcctt gccaacaata atatcgcact
    14221 ttactgacac attagatgtt acgcttgcca gatattcacg ggtagaggtt ataagggcat
    14281 ttattcccgg ggtttcggat ttacctgtta catgaatgca gtgaagctca acctggaatg
    14341 gtgaaacgat tttaaggagt ttacgaatag aagcattatc gctactatca aaattggtaa
    14401 tgtaaagcac ccttttaacc tcgtcaacac ctttaagaac agccttttcg ggtattacga
    14461 gtagtggaat atgggtttcg tccgaaacct cggcagccac gctacctaca agttcagtac
    14521 tcctatagcc ttcaccttta gtgcccataa ctattattcc gggcttatag cgcttactgg
    14581 cctcaataat gccgtaggcg gcatatccct cgcgtaaagt gtatcctatt tgcacttcgc
    14641 caaaccccat ctgattggca aattctaaaa tattttcatg gaattttacc aagcgcacct
    14701 ttgcggaatt ataaagcacg tggtagttca tgtcaaaatc aatttgaatg cttgacacat
    14761 cggtaaaagg tatcatatca accaccggtg agttaaatac gtgcaatagc ttaatttgag
    14821 ctttatactt agaggcaagc attacagcat aacgagcggc attatgcgaa ggtactgaaa
    14881 aatcgactgg tacaaggatt ttgcgcagct gttgatctgc ctttttaatt tcctcatcgt
    14941 ggtgcattgc cgatagtaat ttcaaagcct tttcagcatc tgattcccgg attagcacca
    15001 tcaccccctc ggctatggct ccctgcagta cgttaacact cctcaaaaag cactccacac
    15061 catttgcctc gaggtacgat tttagtacca aagcttttga attactttca gttgcaatga
    15121 ctattagttt atcttccatg gcattttagt ttttaataaa tttaaacaac tgaacaataa
    15181 aagtcaagtt ttttataaaa gaaaaaaggc tgcaaatatg cagcctttcc ccattcactg
    15241 gttaaactat ttaaatatta agttgctaaa caaatcgtca atgaaagaat aaaatctttc
    15301 agaaactaaa ggcataaggg tctcagcctt tacctgttca ccattagtaa acttaaagta
    15361 aagaacccaa tatccttctt cctctacgaa cacgaaatca ccaaccttat cgccaccggt
    15421 tgtatatacc gacaacgatg caaagtcgtt aggatttcca gtttcaagtt cattgccgtt
    15481 tccatcaatc tttaacctga attcaaggtt accaattacc tgtttggcag taattaagat
    15541 atcggccgat tgttcgctta caactgttat ggtcaaatca gcatcctcac tataaaagag
    15601 cttgccatcc ttcttaaagg tgaagttctt actgtaaaga actttattgt ttactgatgc
    15661 atcgaatgat tcttcattag aaatttcaaa tggcccaaaa gtaacggtta ccttagtgct
    15721 gtatttcatc atgttagcaa tggtagcaga ataaaccatg ctaaacacct ggttgccatc
    15781 cttttcaatc ttaaccttaa taccagttgg aacagtttca ccgtaaacca gggtggttgt
    15841 aaagtcatag taggtaatgg ttacgttgtt ggtagcatta gttgccgggt aagggaattt
    15901 aacaataacc ttgtcggttg gagtagattc atgtgtccac ccatcaggag cccaggtcca
    15961 ggttccttta acatcattga aaaactcaat tagaaaataa tcaaaatgga attcaatttc
    16021 tgggttctct agcagtaggt tctgacgagt caactcgtaa gccttttgaa agcgcgaggg
    16081 tacaacgctt tttgaaacat ctgattttac gtagaatggc gaaggcaact gggttgcaaa
    16141 atattcctga accttgtacc catcggtagc aacaatctca ccactcttgg ttagtaactc
    16201 ttgctcagca ttggtaattt gctgctgtgc ttcatccttg ttcaaatctt ttgtatcatc
    16261 cttttcgcag gcaacaaaag ataccattcc tgccaaaaac agggcaaata aagtgaacct
    16321 tttcattttc atagctttaa attttatagt tattaaatta aacatacaag caagttaatg
    16381 caaatggcat gccaaagcaa ttttttcagc tgttttatgt caatcgatac atgaatttag
    16441 tcaacactta tggtgaaatt gtcaacaaaa catgatttta agaaattgct ccttagttca
    16501 taaataaatt tttctatttt tgttggttaa cataaattaa ggattggaga tgaaacagtt
    16561 tctttcatat attttattaa gtctgaatgt tatagcttgg gctgatgcta atgcttgtac
    16621 caactacttg gtaacccggg gcgcaagtgc cgatggttca accatggtaa gctatgctgc
    16681 cgattcccat atcagatatg gggagttata ctttaaacca cgtggcatat ggcctgcagg
    16741 tacaatgatt accctttacg ataggggaat aaacaagccc cttgggcaaa tccctcaggc
    16801 ccccgaaacc taccaggtta tagggtttat gaatgagtat caggttgcta taggtgaaac
    16861 tacttttgga ggaatagagg agttgatgga ctcaaccgcc attgttgact atggtagttt
    16921 aatgtttctt gcactccaaa gggcaaaaac tgctcgtgaa gccattaagg tgatggttga
    16981 acttgtagat cagtacggtt atgccagtag cggagagtct ttctcaattg gcgatcccaa
    17041 tgaggtttgg atcctggaaa tgattgctaa gaaaaccaac ctcaagtatg ataaaaaaac
    17101 aaagcaaaac gtaaatattg ataagggtgc tgtttgggtg gccattcgca tccccgacgg
    17161 ttatatttca gcacatgcca accaggcaag aattcagcaa ttccctctcg aaaaccacaa
    17221 aacttcaatt agtagcaaaa acttgaactt aattttcaac ccagaggttg aaacggttta
    17281 ctcacacgat gttattagct atgcccgtaa ccacggttac tttaacggtt ccgacagtga
    17341 attcagtttc agcgatgtgt ataacccaat cacctttgat ggtgcccgat attgcgatgc
    17401 ccgggtttgg gctttcttta accatgctag cgatggaatg ggtaagtatt gggactacgc
    17461 caagggtgaa aacctcaaga acagaatgcc cctttacgta aaacctaacc ggaaattaac
    17521 cccacgtgat ttgatgacct ttaaacgcga ccacctggaa ggcactgaac ttgatatgag
    17581 caaggatgct ggagcaggtc cccatgggct accttaccgc tggcgaccac taacatggaa
    17641 tgttgatggt gtaacctact ttcatgagcg tgttacggcc actcaacaga caggtttttc
    17701 gtttattgcg caaatgcgca gctggcttcc caacccaatt ggtggtattt tttggtttgg
    17761 ggttgacgat gccgcttcaa cagtttacgt tccaatatat tgcggaataa gcagggttcc
    17821 cgaaagctac tctgagggta atggcgatat gctaacctac tcccccactt cggctttctg
    17881 ggtgtttaac cgtgttgcac attttgcata cctacgctac gatttgataa tgaaggatgt
    17941 aaaaaaatta caggacgaac tggaaaacaa gtttgaagcc tatacacctg caattgatgc
    18001 agcagctcta aagctttggg aaaacaatac taaccttgcc attgaatttt taaccgatta
    18061 ttctgtaaac actgccaaca gcacggtgca aaaatggaac gacttaagca actggctatt
    18121 agtgaagtac attgatggca atgtgaagaa ggaaatagat ggtaaattct tacgaaaccc
    18181 atggggctat cctgtcagtc ccgcgcagcc tggataccgt gaggaatggt taagaacaat
    18241 aataaacgaa actggcaata agtttaaagt accaaatcaa aattaacagt aactcaaaaa
    18301 tcaaaaccaa tgaaaaaatt ttacacacta ctgctacttg cgctctcagg attacttgtt
    18361 attgctaatc ctgttgacgt aaaattggcc aaaaaggttg ccattaacta cctgagcgca
    18421 aaaaaggggg ctagtattga taccttcgat ttaaagctgg taaacacaca tcagtacgaa
    18481 ggtaaagatg ccctttacat ttttgctatg agtaaagggg gattcattat agtatcgtcc
    18541 gatgatgagg caaagccaat tattggatgg tccattacca atcaaatgcc aaaaaagatt
    18601 gataaccctg tggttttaga gcggtttaac tggtatgcta agcaggtaaa ccatgcagca
    18661 aaatctaaaa ttggggacaa gtcggttaag caggaatggc aggatatact tgatggtaag
    18721 attgctaaag gcgttaaagc tgctggtccg cttttaacaa ccatttggaa tcaagagcct
    18781 tactataata tgctttgccc tacagggaca ccaacaggct gcgtagccac agctatgtcg
    18841 cagataatgc gttaccacca atggccacaa agcggtaagg gttggcataa atacgaacac
    18901 cccacatacg gaacccaata tgctaacttt gaatcaacaa cctacgcttg ggctagtatg
    18961 ccaaatgagt taacttccgc aagtacttac gatgagaaag ttgctgttgc aaccatttgc
    19021 taccatgccg gagtttcagt aaatatgaac tatgcgaccg atggttcagg cgcctttagc
    19081 agagatgttt tgtacgcgct tacaaactac tttaactacg acccaaccac tattcaaatt
    19141 tacaccttta accctgataa ccaaaccgaa tggataaata tggtgaaaac cgaaattgat
    19201 gctggcaggc caatttacta ttcgggtagc agttcagcta gtggcggtca cgcatgggtt
    19261 tgcgatggtt ataacgacaa taacgagcta cacattaact ggggatgggg tggatatgca
    19321 aatggatact atgctgcaac agctatgata cccagcggaa ctagttacga ttttagcgaa
    19381 agtaacgata tgattatcgg catccaacca aaccaatcag aatttaaaca cctttgggta
    19441 cggcaagcaa gcggttttgg aacctcatcg cgcggaattc agtacatatc ggcagtaagt
    19501 aatagggttg catgggcagt ggctttcgac ggttcgggtg gtaatgctaa ggttaaggag
    19561 tataccatga ccactgatgg tggagctacg tggaaagcag gttcaattaa ccctgcaaac
    19621 tctgcagggt ttgcagcgag tatgatatgc gcaattgatg acaaaaccgc ttgggtgccc
    19681 ttatatgatg ggacgaatgg gggtggaatg atagccaaaa ccaccgatgg tggaaaaacc
    19741 tggacacagc aaacttccgc aactttcact aagccaaacg gcttccccaa tgttgttcat
    19801 ttctgggatg ccaataacgg cttttgcatg ggcgatccta acggaggata ctttgaaata
    19861 tacaccacaa caaacggagg gtcaaactgg acaagagtat cccaagccaa cattccagca
    19921 cccctcaatg gtgaatacgg cgtagttggc tactatgatg ttattggcga taatgtttgg
    19981 tttgccacaa ataaaggtag aatatataag tcaacaaata aaggcgcaag ctggcaggta
    20041 taccaaaccc cattaacttc aactgccttt gaaattgcat tcaagaatag caatgaaggt
    20101 gttatttatg gatgggaaaa taacattgat aagtattata gaaccactga cggtggaact
    20161 acttggactg aattcacccc aagcggaagt gtatacactg ctgatttatg ctggatacca
    20221 ggtactgata ctttaatatc gactggttcc aactactctg ccaataaaat gggcgtatcc
    20281 tatagtgttg atggcggtaa tacgtttacc gattatgcac ccttttacaa gaatttccag
    20341 tttacaaata tgggtgcctc gccaaatggc acagtatggg ctggaagttt taactcaagt
    20401 gctaactatg gtggaatgtg gaagaaaggg gcatcggtac tttcagctaa tttcaccgta
    20461 aataaaacta ctgcttatcg caacgacagt acggttgtat ttaccgatat gacctatggc
    20521 tcgccagaat cgtgggagtg gaactttggc gagggtgctg aacctgctat aaaaacaggt
    20581 aaaggtccat ataccgttaa atacaccact tacggagata aaactgtaac cttaaccgtt
    20641 cagaaaggta acgatattca tgtatatgtt aaagagaaag tcttaaacgt tacctggccc
    20701 tcaggggttg aaactaacaa tagtgataat aaacttactg tttatccaaa cccaaccaat
    20761 tcatcggttt ttgttaacat tgccgggttt acaaaaggta taattgatgt ttacaacatt
    20821 actggagcat tggtatggac ctcgggaaca gaaacaacag aagggaaaat agatgtatcg
    20881 aatctttcta cgggtgtgta tcttatcaaa attaaaggcg ataatggtac ggttgcaacc
    20941 cgtaagttaa ctgttactcg ttaaaaaaga actaatgaaa tgaatgaagg gggcttaaaa
    21001 aagtcccctt ctaattttta ctttcaaacg atttgaaccc ctaagctttt catttcgtta
    21061 atggccgtct caccatcagt tggattaaca ttaaccgcct taattgcttt tagggctaac
    21121 gttaccttaa atccaagctt tatggcatca gttacagtat ttttcacaca ataatcggtg
    21181 gctaaaccgg caacaactaa ctcgtcaaca tctatttgac gtaaccacga tgctaaacca
    21241 gttccctgaa aacctgaata tgcctccata tcgggctcgg tggctttaga tacaagaaaa
    21301 gcaactgagg gaaagtataa gttggggtga aaagcagcac ctggagttcc tgcaatgcaa
    21361 tgcggaggcc aaaccccacc attctctttg aatgacaaat ggttagctgg atgccaatcg
    21421 cgggtaaaaa tgattggtaa tccatcggcc tcaaattgtt tgctaagctc gttaatgggg
    21481 tcaaccactt tatcgccatc ggttactgcc aaggcaccgc ctgggcaaaa gtcgttttgc
    21541 acatcgacca ctattaatgc tttcatagta ttcactcgtt tgggttatac ctttgcaaac
    21601 gtgggtttgg cataaaatac ctaccgacct tagccatggg tttaccatta acatgaacaa
    21661 tatcggcagt aaaatcgtaa cttccctgca tgagataact gccaacacca taggcatcaa
    21721 ccggaacgcc cattccctcg aattgcctta tacggtcagg gttaaaacct cctgaaacca
    21781 caatctttac atttgaaaat ccttcgcggt caagggctga acgcacaagt tctaccaact
    21841 tggggttaac cccggtaggt ttgtaagttc ccattaatgg gaatacgctt ttatcgacaa
    21901 gattctcaga ggtgtcgagc ctaacacccc ataacttgtc gcccatggcg cgggcacact
    21961 ccaaagcggt atttacgcaa tcgttatcaa aatcaaccaa agctataagg gttgcattag
    22021 ggtatgtatc atgaaaaaac tgaaccgctt taacggtatt gccaccaacg gcggctatta
    22081 aggcatgagg aatggttccg gaagcacctg ctccccacca ttcgccctgt gcatcggtgg
    22141 aaacgcccga tgcaccgcca atatgagcag cataaccatc gccaccctga acagcccaat
    22201 ggtcgaaccg ggcgggaaag tacaaaactg ttttaccgtt tgccgcttca acaacccttc
    22261 tgacattggt agcaattttg gtacggcgtg caaggatacc caagtatata gtttcaagat
    22321 gtgcaaataa cgaggcatcg ccatggatat gcataactga ttcatagggc gatatggaat
    22381 cgccatccca tagggcatca atctctattt ccgaaaaacc atcaatccat aggttatcca
    22441 actcctgcga tactttaata cgctcctcct caatttgcag atacctattt ttatcggtta
    22501 aaaactgttt tctggcaagt ttctttaaat cgattagttt atcgaaaagt tggtatgctt
    22561 ttgagtaatc ggaatatcgg ccacttgcca ctttgagaac agctatagcc tcatcaatac
    22621 cacaaagtat agcgttattc ttttgaaaaa cctgcatggt taccctaggg tgaaggtcat
    22681 ggttttccag aacaactttt tcgcgccaga aatacacatc gctacggtaa ccccggcgga
    22741 gttcctgcac cggaagatcg aatatggtag tgtttaaacg ttttttcata tcttgctaaa
    22801 tttagtaaaa aaggggattg acatcaatcc cccttcatca atatttaaca aatttctata
    22861 gaacaagttc attggcctgc ctgcgttgca ttaccaagcc aacaatgtgt agcttaatta
    22921 ttggcttgcc caatgcgttc gactctaggg taaccgttct acttattcgc tgaggcttag
    22981 gctcaatacg aaattcaacc ctaatttccg attgcttacc tggtaatact ggtgcttttg
    23041 accatgtctt aatatttgtt ccgcagcaac ctgtaacatt tctaatgagc aagggtttct
    23101 tacccgaatt ggttaaatta attagtaaaa caccatgctc actagataaa tcgtcgaggt
    23161 aaatctcccc catattaagc gtatcggctg aaactgttat taaatcgcct ttaccatttt
    23221 taacggaata aacgctaact tcctgtgctc taagagcaaa ggaaaacaga accaaagcgg
    23281 taaatacaaa tacggttctc atatgcatca attttagtaa acatatcgaa ctttatgcca
    23341 aaaatagtaa aactttattc tattcaacac aaatattgat taggccaatg ctaactttgc
    23401 aaagcttaac ttaaaaatcc ataaaaatgg gaattgatct taaaagtttc aatgggttta
    23461 ttttcgatat ggatggcgtt attgttgata actacctata ccatattgat gcctggggag
    23521 aattttgtaa acggcatggg ttgaattttg aagttgaaga ctttacaagg aaatactttg
    23581 gaaagaataa taccgatatt ctgcaagcct tatttgggaa aagcctttct agtactgaaa
    23641 ttgatatgct tggcgaagaa aaagaggcaa tttacaggga attataccag ccctatatca
    23701 aacccctgga cggacttgta gatttcatga aggaattaag aatacaaggg ggaaaaattg
    23761 ctattgccag ttctgctcca caaagcaaca tcaactttgt tgttgagaaa atatgtataa
    23821 acgaatatat tgatgtttca gtaaatggca atatggttag gcttggtaag cccaatcctg
    23881 atatattcct taaagccgca gaacttttaa gtattcaacc tggaaaatgt cttgcatttg
    23941 aggattcatt ttccggaatt caagctgcaa taaatgccgg tatgcaagtt gtagcgttag
    24001 ctactacaca taaaaaggaa gagcacgatc ctactataac aataattagc aacttcaaag
    24061 aattactcca gggatattga cttttactgg atgtattcat atattgcaag aaaaaaacat
    24121 gaggtttctt gcatttattg caattttact agcatatagc acaataacag cgcaggaaaa
    24181 ggagattgat ctctactccc tctcattaga agatttgata aatattgaag tgtcaatttc
    24241 caccaaaacc aaagttgggc ttagggaaac cccgggtatt gtaacaatca ttactaaaga
    24301 ggaaattaag agaagtggag ccagagatat aatcgagttg tttcatcttt ttgtacccgg
    24361 atttgatttt ggtgttgatg ttgagggtgt agtaggaatg ggaatccgag gaatttgggc
    24421 gcatgaaggc aaataccttt ttatggtaaa tggctttacc atgaatgacg gaatgtttgc
    24481 atgtgttcca tttggaaatc actttgcgct tgataatgtt gaaaaaatcg aaattattcg
    24541 tggcccagga tcagccatat atggtggatt tgccggactt ggggttgtaa acataatcac
    24601 ccgcgatact tatgagcttg gcggcaaagt gacctacacc gccacccata ccggaaaaca
    24661 gtttagccat aaccagctca gcgttggcca aaccattcat gacaaagatt tgttgatgaa
    24721 tatttcagcc acctatggtg caggctcccg aagcgataga gcctacactg attactaccg
    24781 gaatagcaga aatctactaa aagcaagcga cacctataca aaacaactag cattaaaact
    24841 tgactataaa ggcttcacaa ttcaaacttt tttagatgat tacctttact accaaataga
    24901 tttgtgggat gaactatata ggggtacacc agttcatgag agttttaaaa gcaactttgt
    24961 tgatttatcg tataaatttc agagggcaaa tttaaacgta atacctcata ttacctacga
    25021 atggcaaaag ccatggcaag taaacattcc agaaataaac tacagcaaca ataagatatt
    25081 ccaaagtctt acacccggca ttagcactta ctacagcaaa aagaccatac aatttgttaa
    25141 tggaattgaa tttaaaactg acaggctaag gctgcctagc atggttgata ccctttatga
    25201 agaaaccttc aagaatggaa aagacttgct atcatacacc aatttttcgg catacagcca
    25261 attactttgg aactcaaaat atgtaaacct aaatattggg gctcgatacg attactcaag
    25321 tgaatttggc gatgctttaa ctccacgaat tggattaaca aaattgttta agcgatatca
    25381 cttcaagctg atggggagta aatcgtttag aacgcctggg ggaatattgc ccaaccgtat
    25441 tccaaacgat tatcccaaat tatatcccga gtcaggtatt acctttgagt ttgagaatgg
    25501 tttcctaatt tcaaataatt cgatgttaac cctaaaccta tatgacatca catttgagga
    25561 tattatcata tatggaaaag atgctcaatc gggagtagga tactacaaaa atcagggtaa
    25621 aattggaaca agaggagtgg aaatggggct taaaatgaat acgaacaagg tattgagcac
    25681 cataaatctt gcttactatt ccaggaaaaa ctctgattcc gacagcctat tcatggtacc
    25741 agataaaaca aatcagtatt tagcgctttc accattaagg attaatggat taataagtta
    25801 caagatttca aacagaacat ccttttcagt aacagcatcg tactttgggg agaggtattc
    25861 atacaactat attgctaata ataacgatat acttgttaag caaaagacaa atctactggt
    25921 agggatgaat atgatagttt ccgatttccc atttaaaaat tttgaaaccc aactaattgc
    25981 cacaaatctg ttgggttcag atttttacta cttacaaccc tacaaagggg cccatgggcc
    26041 actgccgggc ctagacagga gttttggatt tagagtgatt tatgaatact aagaaagcga
    26101 ctcagtgagt cgcttccccg gtaataatat ttactttcaa attatttttc accttcaggt
    26161 ttaacctctt catcggtttg gtttagaacc tctaaaagcc ctttgctttg gagcaagtta
    26221 taccaaccaa taatcttttt aatgtcactt gtgtaaaccc tgtccctgtc aaactcaggt
    26281 aaaacctttt caaagtagct tcgaatttcc ttatcggccg atttgttatt aatggctggg
    26341 cctccgtttt caagcacttc aatctttttc agaacatctt tcaaggggat ttcctcggtt
    26401 tgggtgaata ctgcaatgtc agcaattgca ctaaccctag ctgatgcggg gataagtgta
    26461 cgtttgccgt cggtaagcga ttcaacaatt accccttggc gaccctgaga aatgaacctg
    26521 aataaccctg aatatccaga tattgctagt agttctttaa gttttacttc cataataaaa
    26581 cgagttgttt gttaatgcga agttaattaa aaaatttagt aatctaactt tttttcaacc
    26641 taccatagta atactttgcc caaaccaagt agaatgtagc aataatattt accagatagt
    26701 ttaaccataa aggccattga tactttccga caaccatatc gttgtaggca atatatacaa
    26761 aagtaaatag tactccaaat gcccacatca ggagaaaaag ccagttaaag ctttgtacat
    26821 catgtgtccg ccacgtttga accacttgag gtaatacgcc tatactcagt atgatattac
    26881 caaaccagcc caccgcttca atgaaatcag ctgtattcac tgtagtgtag atttagttaa
    26941 accctcgttc ttttttaatc ttctcataag cctcattaac catcttaaat ttttgctcag
    27001 cagcctgcct gatatcttcg cctaggtgtt gtactttatc gggatgatac tttatggcca
    27061 tttgcctgta tgcctttttg atttcctcgt tggtagcatc gggtgaaacc tcaagtattt
    27121 tgaaatactt atcggtatcg ggtataaaca ttgccttaat ggaactgaaa tcggcatcgg
    27181 aaatatcgag atagccagca atttcaataa ttacttttag ctcctcagag ctaacaacac
    27241 catcggcctg cgctattcca aaaaggaaat gcaacagctg taagcgtgaa tggtagtcga
    27301 cattctcctt aatctgtaaa cagacatcgc gaagcggaac agcctgctta aggatatcgc
    27361 gaagcagaag aattgcctca cgagcagccg ctggtccaaa attggtatag aagtaactct
    27421 taacatactc cagttccgat ttaagcacct tgccatcggc tttcataact gccgagacca
    27481 acacaagtag gcttacaata aaaccattac ggctagtttc gccagtataa atcttagtgg
    27541 tttccatact atcgaatatg gaaccaatag caaaacccag aatggcacca attggcccaa
    27601 aaagtgccca tcctagccca ccggttatcc acttaccgta cttacccatt tttaattgac
    27661 gttaaaattt gaacaatgcc taatacctgt ggtaaaacga gttgatttga gctgttatca
    27721 actatgaaat cggctagttt tttcttttgc tcctcgggca tttgttttgc cattcgcgct
    27781 ctgacttcca attctgtaac tccatcgcgc tcaacaacgc gtttaattct gagttcctca
    27841 ggtgctgtta caagtatagt tttgtgaagt tcacggaaaa ttccggtttc aaatagtata
    27901 gccgattcct taaagactaa cccactttta gcgttttgca agcaccattg ctcaaaacta
    27961 cgtgctacgg ccggatgaat aatactgttg agttctttca gcaaggtgtc gttggcaaac
    28021 actagctcgg caaccttctt gcggtttagc ttgccgttgg ggtaaatgtt gttaccaaaa
    28081 agccctttga ccttttccat aacttcggga tcggtatcag taatggattt agccactaaa
    28141 tcggaataaa atacaggata acccaaaacc tcaaggattt tacaaaccag agttttacca
    28201 cttccaatac caccggtaac accgacccta agcaacatta ctgtttcttt tttactataa
    28261 caaaatcgac cttacgggga tctatctgaa tatttccaat aaaatcaggg tacctaacaa
    28321 gcttcacaat ggctttgcca gatgattcca ttacaaggtc attgtaatcg caaatgattg
    28381 aaaaatgatc gggttttaaa gcaaaatact tgctaactgc cacgttacag gtaacggaaa
    28441 cagtttttgg cgagagcata acctcatatc cataaggggt attgatgcaa gagattggca
    28501 cattaacccg cgcctcggta aatttctcca caggaattga aatacttacc tctgaaggct
    28561 taaaacttaa gccctttatc ggtttaattg ggatggttgt attgatatta tcgtagatat
    28621 cctcgagggt caggggttgt gtataaacac agctaagtgt atcgatgatt gaggcgggcc
    28681 cagtaacctt tactacctta ggaacaacag tatagctgcc agcctgcata tgctgtttgg
    28741 agtacgtaat attcaaatcg ggtttaacgg gaactctttt ttgaacaaca tcgctcaggt
    28801 ggaagtaaag tgtatcaggg ctgacttcaa gtaaagtaaa atcggtccct agctgcgagg
    28861 taaagttatc cagttgtcct gaaagaacaa catagcaatt ccctttcaca aagtcagcac
    28921 cttgagcaga tactgatagt ggcttgtaat atttaaaaag cttatacctt aacaaattat
    28981 accccaaagc ctttactcta agggttaccg ttaggctatc ggatgttaaa gccaccttag
    29041 tttctggcaa ctcggataaa tttactttat aggtaatggt tgatacgtac tcatacctta
    29101 acttaataag taaccaaagg attatggata taaccagaaa gaaaagaaac atgtatacac
    29161 gcttgttcaa gcggatttta tcgggctgga aaaattgatt gtattttttt atggattgtt
    29221 gattcacagg gaataattca attgattttg gaaaccaaaa gtaaattatc ttggtaaaaa
    29281 aacctaaggt tgtactaaaa ttattagggg ttatcaagtt gaaataaaaa aaggattcaa
    29341 agcttctctc aaaactttga atccctcgga aagaaaatat ttatcgagtt tgcatatcgg
    29401 tcacatcacg aataatagta ctcttggcaa cctttacttc aacattatcg gcaatttcaa
    29461 ccaaaacata ctcatcttta acctcggtaa ccttaccgta aacaccgccg gcaataatca
    29521 ccttatcacc tttcttaagc gactcctgaa acttacggag ttccttttgg cgcttcattt
    29581 gaggcctaat cataaaaaaa taaaaaacaa caatgatgag accaaacatc aagaaggtca
    29641 tcaacgggtt agcaccctgc tgggttgctt gaagtgtaat aaaacttgta atcataactt
    29701 tatcaacttt aattaattaa caatttcagg tttataaaca tacaaagtta gtaaaatgtt
    29761 ttgaaaatta gggtactaca tttgccttga gggttactga cttttcacga ataaccccat
    29821 tggttctaag agtaaccgat ttatactggg aaccaaacca gcctttacta tcaaaaacca
    29881 cctcaacgga tgcctcctcg ccgggtttaa gaattctgct ggtaacatca acctttgtgc
    29941 atccgcacga tgggattata tccttaacta ctagaggcgc attaccagta tttctgaagc
    30001 ggaacgagtg cctaacaatt tcaccgctgt gtattgttcc tacatcaaaa aaatcctcaa
    30061 cataggtgag ctcagctagt tgggttgagt cagtctcctg agttgaaccc tgattgttat
    30121 tactaccaca cgatattgca aataaagcgg taaaaccaaa aagcaagcta gttatcctca
    30181 tcttcaaatg aattaatatt ttcgggtgaa agactttcgt cgttttcgcc aatcaaaccc
    30241 ctaccctgct tctggatttg tccattctcc ttcaggtgag cgattacctt atcgagaaca
    30301 ccatttacaa acgagttgct ttgctctgta ctatagaact tggctatatc aatatactca
    30361 ttcatgctaa cctttacagg gatggtagga aattcaagaa tttcagtgat agccatttcc
    30421 ataattgcaa tatccattgc ggctatgcgg tcaagctccc agttaacagt gaatttatca
    30481 ataagctcca ggttttcctt atgccttaaa accgttttgc gaagcaaacg tttagcaaac
    30541 tcctcgtcgt cggcattgct aaaaagcgac ataactggaa ccgatgctgt attttcatca
    30601 atcttcttaa ttgttttggc agccatgctg gaaacaaact cgaggtcatc gttccagtat
    30661 atgctttgct cctcaaggaa tgcctcaaaa tcgtcattag catttaatat cttgctgagt
    30721 agattaacca gaactgcttt atcgtcagca aaagagttat gctcagcatt catgtaatcg
    30781 gaaaagaaat ccgatgccac aaagtggttg aaaagcgatt tggttacctc agggaaatgg
    30841 ttccaattca agcgattatc gtttgctttg gcttgtagct ctttgcaggc agccaatgca
    30901 gcaactgctc tgttctgaac aaaccgcagg ttggggttca actcttcagg cgacgggcgg
    30961 aacttttgct tacccagctc aatttttgcc tgggcatgct caaccatatt ctctggcaaa
    31021 agcagtaaaa gcaagtaaag atggtacgat ttttcaatgc tatgaaacag ctcgcgctct
    31081 aatgcgctaa gagagtcacc ttcgttacgg aagaaggcaa aaagaacctg taaggccttg
    31141 attctaatta aacgacggtt aatcattagt aagaacgatt tttttcactc ctgcaaagat
    31201 agaaagatta atccagtgca gtggaataaa taaggttaat atttcaaaac aaaacctttt
    31261 gggtgttttt taataatttt tttaagtcaa tgaaccatga tatgaagatt tttttaattt
    31321 tgagcagtgc aaaagtgaaa ccctaaccta aaaatactaa gagcaatgat tgaggaattt
    31381 gataacacta acgatttaga caagaacacg agagaagagg ttttttctca ggtagtgaag
    31441 gccggaaaaa gaacatattt ctttgatgta aaggcaacac gcggcaatga gtattacctt
    31501 accattacgg agagtaaaaa acgtatgggt aaggatggca agatgtacta cgaaaaacat
    31561 aaaatcttcc tatacaaaga ggattttgaa aagtttatcg aaagcctgaa tagtgttttt
    31621 gactacatta aagctaatca ggaaattgtt ccacgtactg atcataccga tgaaatggaa
    31681 ctttctgaag ttgaatccga ttactcatca gttgactttg acgacttagg taaatagttt
    31741 tttataacga ttttcgaaca gtacagccat tcagtaatga atggcttttt tacttaaaat
    31801 cgatctaaat aacagaaaac tcttacattt gtaaaatttt agtaattcca aaactttttt
    31861 gaaacattga ggttttactg ttactgattt taaactagat gctactatcg gagttacaga
    31921 acggggaata cggcataata gcaaaagtaa agggacgcgg ggcctttagg aagcgtatta
    31981 ccgaaatggg ttttgtgaaa gggaaaaaag taactgtagt taaaaacgct cccttaaagg
    32041 atcctattga atactcaatt atgggttacg aggtatcgct ccgtcggagc gaggcttcgc
    32101 taatagaggt tattacaccc agtgaggcta agcaaatttt aaactatcat cctgataacc
    32161 atcagcttgt tacccacact gaacctgaaa ttattgacac tggggtaaag gaacttagca
    32221 aaactatcaa tattgcgttg gttggtaatc ccaatgctgg gaagaccagt atctttaatc
    32281 accttgctgg agcaagggag catgttggaa attacagcgg ggtcacagtc gattcaaaaa
    32341 acgcaaagtt taagtataag ggatatacaa ttaacattac cgatttaccg ggtacctact
    32401 cccttacagc ctatacaccc gaggaggtat ttgtacggcg atttatccat gagcaaaacc
    32461 ctgacattgt cataaatgtt attgactcat caaaccttga gcgaaactta tacctaacca
    32521 cccagcttat tgatatggat atcagggtga ttgttgccct gaatatgtat gatgaactac
    32581 ttgagaaggg cgacaggttc gattacacca tgatgggcaa actacttggt attccatttg
    32641 tacctaccgt tggtagcaaa ggtaaggggc tcgacaggct tttagataca actataaatg
    32701 tttatgcaga aaaggataaa gttacccgcc acatccatgt taactacggt gaagagattg
    32761 aaaaatcgat actatccatt caaaatgaga ttaagaaaaa tacagctata accatcaact
    32821 attccagccg ataccttgcc attaagctgt tggaaaaaga caaggtagca gataaagtgc
    32881 tgcgcaatga accaaactac caggaaatca gaaaccaaac cgaccgtgag gtaagcaggc
    32941 tcgaaagcct tttccacgag gatacggaaa ccctaataac cgatgccaaa tatggcttta
    33001 tagcaggtgc actaaaggaa acattcaagg agagcagcca caagaagcgt ctggttactg
    33061 aacaaatcga cgcaatactt acccaccgtt tactgggatt ccccatattt attttcctga
    33121 tatggctcac gttttacacc acattcaccc tggggcaata ccctatggag tggattgaac
    33181 tgggagtgga atggattagt aacaccatta gtacatacat ggccagtggc cctttaaaag
    33241 accttttggt aaacgggatt ataagcggtg taggaagtgt aattgttttt ctacccaata
    33301 tcttgatcct tttcctattc atatcgctac ttgaggatac tgggtatatg gcacgtgctg
    33361 catttatcat ggataagctg atgcacaaaa ttgggctcca tggtaaatcg ttcatacccc
    33421 ttgtgatggg gtttggatgc aatgttccag ccataatggc ctcgcgaacc attgaaaacc
    33481 gcaataaccg attagtaacc atgctcatta cgcctttcat gagctgtagc gccagattgc
    33541 cggtttacat acttattctg ggtgcatttt tcccggaaaa cgcaggaaac cttctgtttg
    33601 ttatatactt tacgggtata ctgcttgcca ttggaacagc cctagcattc aagaaaacca
    33661 ttttcaaagc ggaggatttg ccctttgtaa tggaacttcc accctaccgt atgacaactt
    33721 tacgatcgac cctattgcac atgtggcaca aggggtcgca atacctaaaa aagatggggg
    33781 gagtaatcct ggtagctgta atctttgtat gggcattggg ctattaccct cgcaaagtga
    33841 actacaccag ggattataat gccgaaattg aaactattac gcaaaagatt tcggaagaaa
    33901 agctctccca accctctatg gatagcctaa accagacaat acatcaactt gagttggaaa
    33961 tggaggccga aaggcaagcc aattcgtatc ttggacggat aggcaaattt attgaaccag
    34021 ccattcgccc tctcgggttc gattggaaaa tgggtgtaag tcttctttca ggagttgctg
    34081 ccaaggagat tgtagttagt acgctgggcg tactttacca aaccgatccc gattccgaca
    34141 gcggtggact tgctcaaaaa ctccgaaacg aaacccacca atcagggaaa ttaagcggac
    34201 aggtggtttt tacaaaagtt actgcactcg cattccttat ctttattcta gtttacttcc
    34261 cctgcgtggc tgtaatctct gctattaaga acgagtcggg ttcgtggaag tgggctctct
    34321 ttacaatagt ttttactacc tcgctggcat gggttttagc atttttggtt taccaaattg
    34381 gtaatataat aattggttga tatggttcag gaaatagcag tattaataat agtattagga
    34441 gcagtgggat atactgcata caatgtgtat caaattttta acccaaaacg tataaattcg
    34501 ggtaaatgtg ccggatgttc atcggcttca tgcacaataa gcaatttgaa tcaaccctca
    34561 aaaaaatgaa ctttttgttc ctttttatgt ttagctatat taggtgagat gtaagatgaa
    34621 aaaggttacc caaaaaatag tatcaattac tatagcaact atagtgctac tatccactag
    34681 tgggtttaac ctcttcatac atcattgcca gtgcgagggt gaaattcacc tgtccatttt
    34741 taatgaagtt ggatgcaacc atacacacgc caaaacacat gcacactgct gcaatgttca
    34801 ttttgatgaa acactcaaat ttaacgaaaa gcaatcgtgt ggatgctgga acgaggtgtt
    34861 aaaggttaaa accgatagct actcaaacat tcagaaggtt gaaaccggaa atcttttttc
    34921 aaagtacata gcagtaaacc accacaaggg gctaaataca acaatccata agttaatccc
    34981 aacttacaaa accaattggg ataaagcccc accctgcagt acggggaaaa aaatctgcat
    35041 tgaaaatcat cagctaaaaa tagcccatac aatttaatcg ttacagagag ttttttctct
    35101 ctgcagctct taattgcgct cattttttgc ttgtagcgct catatttgat ttcaacaaca
    35161 atttaagaat ttctattatg aaacgcatat taacattact gttactatgg ttttcagctc
    35221 aacatatcat taaagcacag agtaaagatt ttgtgtacgg ttatgtttac gagctgaacg
    35281 agaaaggcga cagtattccg ctccaaggag ccgccgtaat ttggctgaac agccaagttg
    35341 gtacaactac cgatgaaagc ggttactttt ctttaaataa ggtgcaaggc aaccagatag
    35401 ctataagttt cgttggtttt aaaaccgata ccataagtat tgagaatgta gattttccgc
    35461 tacgccatgt cctgaaatca actataagta tcgatgccgt aacggtaaaa gctgacaggg
    35521 caaatacctt tatacagcag ctaaacccaa tacaaacagt tgtacttaca tcgggagagc
    35581 ttacccgcgc tgcatgctgc aacctatcgg aaagctttga aacgaatgct tccgttgatg
    35641 tgtcgtatag cgatgccatt accggggcaa agcaaattca gctgctgggg ttagcaggaa
    35701 tatacaccca gatgcagtat gaaaatatac ccacaatgcg agggttggca tcaacctttg
    35761 gtttaagtta tgttcctggg ccatggatgg aatcaattca agtatcaaag ggaactgcat
    35821 cggtcagcaa cgggtacgaa agcataaccg gacaaatcaa tattgagtac aagaaaccct
    35881 gggctgagga aaagtcgtac ctgaaccttt acaccaactc acttggtatg gctgaactta
    35941 atgctaacta cgcattcgat gttaccccca ccgtatcatc aatggtgctg gtgcatggaa
    36001 gtatgctaag caaccgggtt gaccacaacc acgaccaatt ccttgaccac ccgcttaccc
    36061 aacagtacca cctgtttaac cgatggaagt accagggtaa gaagtgggaa tcgcaagttg
    36121 gagtgaagtt tatgaatgag gacaggctag ccggacaggt taagtattat accaatactc
    36181 aacccctgat tggtaacccc tacggggtaa agtactcaac ccaattactt gaggccttta
    36241 acaaaaccgg atacatttcc caaagaccaa acacaagtat tgggcttatc aatgccgtat
    36301 cgctccacag tcaggatgca accataggtg ttagaatgca caacgccaaa cagcttagct
    36361 actacggcaa cctggttgca ataacctata ttggcaatac aaaccacaac attaaaggag
    36421 gcgcaaccct caggtacgat aggtacgatg aaactttcaa taacacccct tacgatcgca
    36481 ccgagtgggt caaaggtatt tacggcgaat acacctataa acatctggaa attcttacac
    36541 taatggctgg cataagggtt gaccaaaata gtaggtttgg cactcttgtg acacctcgtt
    36601 tccacgggat ggtaaaacca gtttcgacct taaccattag gttcagcgca gggaaaggat
    36661 acagatcgcc aaatttcatt gctgagaact caaacctatt ggtgagttca cggaacctgg
    36721 tagtagagga aaatccaaga attgaagaag cctggaatgc cgggattagc atgaatcaga
    36781 aattcaaact tttgaatcaa aactatagtt taaccgccga ttattactat gtgtggtttg
    36841 aaaatcagtt cattgccgat atcgacaggg ataacattgc agtttacttc tataatctga
    36901 aaggcaaatc gtattcaaat accttgcagc tggaactttc aggcgagcca atcaggaact
    36961 tgcagtttac cttggcatac cgaataaacg atgttaaaca aaccattaac gatacgttaa
    37021 agcaaaaacc acttgtaagc cgcaacaagg gtttgcttac cgctggttat aaaaccccat
    37081 cgcgtaagtg gcagttcgac tataccattc agtataacgg tagtggaagg cttccaaaag
    37141 ggtatggact aaatcgagaa tcatactata acccttttat taccatgaat attcaggtaa
    37201 ccaagatgtt tcgtaagttt gatttctaca taggcgttga gaaccttaca gggttcactc
    37261 a
//
LOCUS       DAOOWJ010000011        37134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1156_length_37134_cov_7.370614, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000011 DAOOWJ010000000
VERSION     DAOOWJ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 37134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37134
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_37134_cov_7.370614"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..2465)
                     /locus_tag="PLW22_02225"
     CDS             complement(129..2465)
                     /locus_tag="PLW22_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU73550.1"
                     /translation="MAQKKVGEKRFEGSFEYDGELKYRLIFEKSPIGLFQYNTDAVIT
                     ECNDRFVEILKSKRELLVGLNMHKINDKSVLPAILDALDGKNGYYEGPYTTTTSNAQI
                     YVLLKAVPLTNPKTGQVVGAMGIVEDISDLYRTQEELTIKEERFRILSSLTSDSASIL
                     TIQPDGSFKREWLSSALMVQLGYKPEDVDSFEKWATIVHPDDIDVFKRAIGTITQKGE
                     KVTLEFRIKAKNGKVHWIENTVYPEFDSSGKPFRLISAVKDITAKKEQEQELQQQRNL
                     LKSIVDNAPLGIWVVNPDGSYPIINPWFSQNIGYGTSQFSMTEEEVAQCRRSDMQALN
                     SDGPIETTEEVTFTDGKKHILQIFKQKLTSTTGETIGVLGIANDISERTQYEKALVEA
                     LEKAEESDRLKTAFLANMSHEIRTPLNGVIGFAKFLRNFPDTTLQEREKFLGIISTSA
                     DHLLTLINDIIDISKIDVGQLTINPEAVNINELLNEIYTFFYNANPDLPKREIAFNYT
                     TSLPDSKAMVNADRMRLRQVLTNLINNALKFTDKGKVEFGYNINSETNEFEFFVSDTG
                     IGIAKDKQEIIFQRFRQAYTDITKQYGGTGLGLAICKSLVELMGGRIWVESETGKGST
                     FRFTLPINAPTTQNRGKVYGYELQELKNLLKGKSILIAEDDPNCMYYLKTVLKDFDIN
                     ILEADNGLLATEIQYQHPEIAAILMDIKMPVMNGYDAIKRIRSTNAQVPIIVQTAHTF
                     SNERAISIALGCNQFITKPIDSNSLYQSLYSVLKESRR"
     gene            complement(2779..3732)
                     /gene="metF"
                     /locus_tag="PLW22_02230"
     CDS             complement(2779..3732)
                     /gene="metF"
                     /locus_tag="PLW22_02230"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOU73551.1"
                     /translation="MKVIDILNNSKSTLFSFELLPPLKGHNIETIFATIDKLLPYNPT
                     YINITYHREEVVYMSNENGIQVPVKVRRRPGTIGLAAAIQQRYAIDVVPHIICGGFDA
                     HETEDALIDLDFLGIQNILAVRGDADKITGRFEPRAGGHRYAIDLVKQVTDLNRGVYL
                     EKLMEKPKPTNFCIGVAGYPEKHPESPNCESDIHYLKKKVEAGASYIVTQMFFDNKLY
                     FNFVDKCRKAGIAVPIVPGLKPISIKEHLSILPRVFDVHIPQALVLELEKCKTNNQVA
                     QLGVEWAIEQSRELKSSGVKAIHFYTMGKDDNIAQIAKAVF"
     gene            complement(3744..7415)
                     /gene="metH"
                     /locus_tag="PLW22_02235"
     CDS             complement(3744..7415)
                     /gene="metH"
                     /locus_tag="PLW22_02235"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOU73552.1"
                     /translation="MEKTTVFRELLKSRVLVLDGAMGTMIQRYRLKEEDFRGDILKNH
                     PVNLKGDNDLLNLTRPDVIGQIHRAYLEAGADIIETNTFNSTRISQSDYGLEQMVYEL
                     NLAGARLARSIADEFSNANPQKPRFVAGSIGPTNRTASMSPDVERPGYRAVTFDQLVN
                     SYTEQAETLIDGGVDILLIETIFDTLNAKAALFAISEILERRGIDIPVMVSATIADNG
                     GRLLTGQTLEAFVCSVSHFPLLSIGLNCAFGAEQMYPFVEELSAITPFFVSAHPNAGL
                     PNQFGEYDQSAQEMAEIVEQMLANGLVNIVGGCCGTTPKHIELVANLAQRYKPRIIPE
                     IPRHTRLSGLELLEIRPESNFINIGERTNVAGSKKFARLIAEGKYAEALSVAREQVEG
                     GAQAIDICMDDALIDAPRAMTEFLNLIASEPDVARIPVMIDSSRFDVIEAGLKCTQGK
                     SLVNSISLKEGETEFARRAKVIKRYGAAVVVMLFDESGQAVTTEHRCRVAERSYRILT
                     EKVGFSPEDIIIDPNILAIGTGMVEHSTQAVSFIETIKWIKQNLPYAKVSGGVSNLSF
                     SFRGNNLVREAIHSVFLYHAIQAGMDMGIVNPSLLMVYTDIEPNLLKLTEDLVLNRRR
                     DATERLLAYASKLEETDLQHHKTDDWRLLPVEQRLKHSLIKGTDEFIRVDVEEAYNKL
                     KSPISVIEGPLMDGMREVGNLFGTGRMFLPQVVKSARVMKQAVKVLEPYLEAQAMDSH
                     RTEQKKIVLATVKGDVHDIGKNIVGVVLACNGYSIIDLGVMVPAERIVDEVKASKADI
                     VGLSGLITPSLDEMVHVVKELKRNGINIPVLIGGATTSELHTALKIAPEYSGAVVHVR
                     DASQASGIVASLLSTDKSASTINAYQKRYQQQRESYSGSKTVLVPIEQARKNSLKLNW
                     DSYKPVRPKQIGINTLSNIDLNELIHYIDWTFFFRAWGLKGRYPDILTDPVHGSEAQK
                     LKAEALAMLKQISEQKLLTPEGVYGIFPAASSGDDVIVYTDETRTSPLTHLPFLRNQE
                     QKPEGEPNMCLSDFIAPANSGINDWIGCFAVTTGSTTSTLIEEYRKNGNDFAAIMIQI
                     LADRLAEAFAEWLHLKIRKDFWGYSPNESLTVEQLLQEKYVGIRPAPGYPACPDHRGK
                     QWIFQLLDIPRNASISLTENMAMNPLASVAGFYFASSASKYFSVGKIDNDQLADYAKR
                     IGVSTTEAARLFPTFLANQ"
     gene            complement(7427..8743)
                     /locus_tag="PLW22_02240"
     CDS             complement(7427..8743)
                     /locus_tag="PLW22_02240"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOU73553.1"
                     /translation="METLTITNTRKNSKSNGVNQQVIGLFGLGNVGNALYHVIAEIPK
                     SKAYIKRICVKQRHKNREVPHELITYNAADIINDEEINLIVELIDNADEAYHIVKSAL
                     LRGKSVVSGNKTMLAHHLPELIAIQHETGAALLYDASACGSIPVIRNLEEYYDNDLLK
                     SIRGILNGSTNYILSNVFSQGVDYHWALKQAQELGFAESNPDFDVLGFDALYKLIILA
                     VHGFGTYVHPSDAFCYGINNLKAYDIQFAREKGKKFKLVAQLQKLNADYFTLFVIPTL
                     VEPSDYIYNVENEFNGVVVEGQYYDRQFMFGKGAGGYPTGSAVLSDITARFHNYRYEY
                     KKQKYFTPPAYSTDVELELYIRYHNLTDVSIFGERKVTEQHFATDYRWEIAWVKLNEL
                     IRVKELLPKLNLFLAFTGNFRFNPIDKALSSKVTCADDYQPIVDAF"
     gene            complement(8757..9776)
                     /gene="metX"
                     /locus_tag="PLW22_02245"
     CDS             complement(8757..9776)
                     /gene="metX"
                     /locus_tag="PLW22_02245"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOU73554.1"
                     /translation="MKVTEGNLELKTLQLECGEVLYDVNIRYHILGDIQKNPERVIWI
                     CHAFTANSNPADWWPEMVGQGRLFDPEKYTVVCANIIGSCYGSTGPMSVNPKTGKPYL
                     RSFPLVTFRDVVTAHQILKEYLGIDKIWVLTGGSIGGFQAMEWECMYPGTTENLILIA
                     TAAQASPWVKAFSQTQRLAILADGTFDGANPNGGKAGLKAARSIALLSYRGRSSYNLT
                     QQDSDPNQLSDFKACTYQDYQGDKLVKRFDAYSYFALSLMLDSHNMGRGRVSNEAALN
                     SIKARTLVVGIDSDILFPVEEQHFIADHIPSAVYREIHSQFCHDGFLIEYQQLTSIIS
                     EFINL"
     gene            complement(9827..11131)
                     /locus_tag="PLW22_02250"
     CDS             complement(9827..11131)
                     /locus_tag="PLW22_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOU73555.1"
                     /translation="MNNTYHFDTLQVHAGQGVDTNTLSRAVPIYQTTSYLFRDSEHGA
                     NLFALKEFGHIYTRLSNPTTDVLEQRLAALEGGKSAVVVSSGHAAQFIALTNIMESGD
                     NFVSSPYLYGGSHNQFKVTLKRLGIEARLPKSLKPEDFECLIDSKTKAIYVETIGNPS
                     FNIPDFKALTTLANQHQIPLIVDNTFAGAGYLCRPIEWGANIVVESITKWAGGHGTSM
                     GGVIVDAGNFNWGNGKFPQFSEPSEGYHGLVFWTAFGEDSPHGNIAFAIRARVEGLRD
                     FGPAISPFNAFQILQGIETLSLRMERHCSNALALAKWLKTNRHVERVCYPGLPDDPNH
                     IQAKKYLKNGFGGVLTFTLKGNLEQTQTFVDSLKLVSHLANVGDAKTLIIQPAATTHQ
                     QLSEEERLAAGVLPNLLRVSVGIEHINDIIADFEQAFQKTFS"
     regulatory      complement(11382..11485)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11643..12134)
                     /locus_tag="PLW22_02255"
     CDS             complement(11643..12134)
                     /locus_tag="PLW22_02255"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOU73556.1"
                     /translation="MGRFRIEAKWALIFSLMTLAWMVLEKVSGLHGKYIDYQYYLTNL
                     FAIPAIAVMVLALKDKKQNFFHGNMSYLNGLITGIVLSLFIAILSPLTQWIISYVISP
                     EYFPNVIKRSVELGYYKSVAEAEAFFNYKNFAIQSAIGALVMGILTTAIAMIFIRTKG
                     SKK"
     gene            complement(12204..13103)
                     /locus_tag="PLW22_02260"
     CDS             complement(12204..13103)
                     /locus_tag="PLW22_02260"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOU73557.1"
                     /translation="MQKYLLALIAVLAFSFVHGQQAGGKKRQPKGPYPNDWVLVEKGG
                     SFGFISSTGKEIIKPQYTRIHPFDEFQRGWAMVEKDGFFGFISAEGKEIIKPQYTKIY
                     PFGDFNRGWAMVEVNGKFGFISVEGREIIKPIYSKIYPIGEFYKNLAMVEKDGLIGFI
                     SLDGREVLKPQYQKVHPFGEYQKDWALVEKDGFLGFINGEGREIVKPQYSKISQFNEY
                     QKGWALVQRDNYMGFINSEGKEVIKPQYTQIMPFDQVQRGWALVERDGLWGFISSEGR
                     EVVKPKYEKISLNDSTAFQKTRP"
     gene            complement(14180..15754)
                     /locus_tag="PLW22_02265"
     CDS             complement(14180..15754)
                     /locus_tag="PLW22_02265"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOU73558.1"
                     /translation="MKIKIILIIAVAATLVSCQRQGSSDIDSTAYLEEITIKQLQDGY
                     REKRFTVEKVVNDYLKRIELLDKEGPCLNSVIAINPDALKIARELDKELAQGKVRGPL
                     HGIPVILKDNIDTRDSMPTTAGATVLRNSFVGRDSWIAAKLREAGAVIIAKANLSEWA
                     NFRASRSSSGWSGVGGQTRNPYELDRNPCGSSSGSGVAVSANLCAFAIGTETDGSIMC
                     PSNNNGIVGLKPTVGLISRSGIIPISFTQDTPGPMCRSVSDVAYCLGAMVGPDPNDPK
                     TLDPNARFYNDYTQFLRLDGLMGKRIGFITNASGFHHKVDTLMSKAISDLRRFGAEVF
                     EVSLPIPQEVNSAEFQVLLYEFKDGLNRYFAGLGEKAPIKSLEELISFNRTDTIELRH
                     FDQHLLELAQGKGDLNSPEYIKALETMQKGIRQNGIDKVMDENKLDLLIIPTGSPAWK
                     TDLILGDNYIGGNTSYAAMAGYPSITVPMGNIDGLPVGISFIGRAWCESTLIEAAYSY
                     EQGTKHRIVPKFKASK"
     gene            complement(15843..18716)
                     /locus_tag="PLW22_02270"
     CDS             complement(15843..18716)
                     /locus_tag="PLW22_02270"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU73559.1"
                     /translation="MKFLKKIIAFALIAIALQSFAQTQTIRQFTLPNGLTVILDEQHD
                     QPEVFGLIAVKTGGKNDPADATGMAHYQEHMLFKGTTTLGTIDWEKEKPHIDRIFQLY
                     DSLGKTKDPEKRALIQKAINEESVEANKFAIPNEMNNLLNEMGSTLINAGTGPDYTLY
                     YNKFPASQVERWLDLYAHRFTEPVFRGFQAELEVVYEEKNLYNDQFQTRLLEEFQKYF
                     FKNHPYGQQTLIGSIEDLKNPSLTKMYEFFKTYYVPNNMALILSGDFNSEEVIPAIEE
                     KFGKWQPRELPEPRKWDEAPFNGREFHEAKLSPIKLAILGYRAPNATDPRKPLLDVTT
                     RLLNNSYSTGLLDKLTLDGKLLAAQAMMMPYYDHGAIIFIVVPKVVGQKLEDAEKLVL
                     SEIEKLKKGEFDESLLEAIKLEIYRNTNTKLENIQNRALTLSEAFTQGKSIDEALTYP
                     EKVKSITKADIVAIANEVFGPNYLAFYSKMGFPKKEKIAKPDFKPLLSNTKARSAYAQ
                     HFDSLPIRTPNFKFIDFEKDLKVVELAGGHRLQCVENPKNDIFSLSIEFKVGKASLPL
                     LGFATQGMALAGAGEYDVNALKTEFAKLGSSYSIWANENKTTITINGIESNLEPTLKL
                     VGLLLSDPKLEQSKIKTIVANEKATRKMERSEPDNVADALVEYGLYGDESYYTKRLSM
                     SSVKKLKAQELVDAFKQAAGYTATISYCGNTSADKISDIIASYLPLSSSPVVDTTPLD
                     KPAKQYTENTILFVNKPKARQSKMFVFINGKPFEIGDAAYIDAFNDYFGGGFSGLMLQ
                     EIREYRSLAYATGGSFRYPLEKGKPVNFIGYVGTQSDKTLIAMETLDTLIRQMPAKPE
                     RIEMIKSHLELSAQTSRPNFRNIASTVDVWRKRGFPVDPLIIKLPIYRGLTWENIDEF
                     YKDRMKNNPVVYMIVGDEKNIDMKSLAKYGKLVKISEKTLFSK"
     assembly_gap    18975..19074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19093..20112)
                     /locus_tag="PLW22_02275"
     CDS             complement(19093..20112)
                     /locus_tag="PLW22_02275"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOU73560.1"
                     /translation="MQNLDWKNLPFGYVKTKYNVRCYYKDGKWSSLEVSDSEYINIHM
                     AATALHYGQEAFEGMKAYRGKDGKIRLFRWDENAQRLQHSAEGILMAPVPKELFYEAV
                     VKAVKLNEEFVPPFGTGASLYIRPLLFGSGAEVGVRPAKEYMFVVFVTPVGPYFKEGF
                     NPVKIAIVRDSDRAAPLGTGTFKVGGNYAASLRGVEKARKAGYSSPMYLDAKEKKYID
                     EIGAANFFGIKNNTYITPKSTSILGSITNKSIIQLAEDMGLKVERRPVPVEELETFEE
                     VGACGTAAIISPIGEIKDLETGKVYTFCKDGKPGQISTKLYETLVGIQYGELEDKHGW
                     VTVIE"
     gene            complement(20592..21596)
                     /gene="asnA"
                     /locus_tag="PLW22_02280"
     CDS             complement(20592..21596)
                     /gene="asnA"
                     /locus_tag="PLW22_02280"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOU73561.1"
                     /translation="MESKVFETLTGDICRSVETEDAITLIKETFPKLLAKKLNLRRVT
                     APLIVEAGLGINDDLNGVEKPVSFTASSIGKRIEIVQSLAKWKRLTLARLELPCGSGI
                     YTDMNALRPDETPDAIHSVYVDQWDWEKRIDASERCVEYFRKTVNAIYEAICEMEMVV
                     ATHVDGITPELPKSIHFVHSAELEAMYPTLSSRERENVICKQFGAVCIEGIGYPLSDG
                     KPSDGRAPDYDDWSTLRPDGYRGLNGDILVWHKPLGIALELSSMGIRVDSAALKLQLQ
                     MQGLEHRSKLHYHSLLLNGKLPQSIGGGIGQSRLCMFLLRKVHIAQVQASVWPEGQGL
                     "
     gene            complement(23052..23261)
                     /locus_tag="PLW22_02285"
     CDS             complement(23052..23261)
                     /locus_tag="PLW22_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73562.1"
                     /translation="MEITKDISIEELVEQVPEAVSFLREKGIVCIICGEPVWGTLYEI
                     ASQKGFSDLEIEELVEGIARLKDKG"
     gene            complement(23267..23689)
                     /locus_tag="PLW22_02290"
     CDS             complement(23267..23689)
                     /locus_tag="PLW22_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU73563.1"
                     /translation="MDKISIQADWLDGMAFETEVNGHKLIIDADEKVGGTNRGPRPKP
                     LMLVALAGCTGMDVVSILKKMREDLKKFRVSVEALQTDEHPKHYVEMKIIYEFWGNNL
                     NPENVNKAVKLSDEKYCGVSAAYKKGMKLTHEVIIHNE"
     gene            complement(23869..>29565)
                     /locus_tag="PLW22_02295"
     CDS             complement(23869..>29565)
                     /locus_tag="PLW22_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU73564.1"
                     /translation="YLNIEVWDRATVDVSKDNGSTWSNIWYNNNYFTDNLWLKTSHNV
                     PSEVSRSNKLKFMYKLGPTDNYSTYSGWNVDDFTVTGDYITKDVGVVEWVYPLSGCGH
                     SANDSVTVKVANMGAEPSPASIPVQYSFNNGVTWVTNYLNQVIAPGDTVQFTFSTKVN
                     LSTPGIKQVRARTRLTGDEDASNDGISRQIYIVPTYTLPYTETFESNDGYWRKFGNPI
                     WEWGIISKPSMSGGSKAWATSLSQNYGSLLTGVTDTIFYDDFETAKGWTFTGEFERNH
                     IIMQGDTIPTYTYSGQYCIGTDLSRQGVNKGMYEPNATWYAKSPAIDMSNYNNLTLYA
                     WRWHKLAAGDTAMVQASSNGTTWETLFTTGGTAVTHENWDVDTIPIPNGLANSGTLYI
                     RFVLKSNGDANVAEGINFDDVWITGSRFNTETAWLQSPCFDFSGVTNPIIDLNVFNRT
                     EPEVDGATLYYSIDNGQTWQHVGNNATHDEYFNWYTDSLVSALGVDGWNGLGAAWERS
                     RHELPATLAGKSNVIFRVGFKADKANNNYGGTAIDNIKLYEAPFDIGVESILTPSTAC
                     DLNQNQPIQIRIRNYGIRNMIAGDTIVVGININHSMVPDSETDTIVLTSALNTGDHLD
                     YNFSRAFNMNVSGDYNLTASTSIETDPLFYSSPSNDAATKLVTVQKPYVELGPDIWTL
                     QPDTVVLDATNPDPLVTYKWYKAPDKTNVISTTPTDTVSDPNGGKYYVLLENSIPCQA
                     WDSITVHRLIRDVGIPAFDSPVSSCEPSDKTPIKVHVKNFGTDTLTIGDTIRISYKFN
                     SDPKVDTVWVSDRIILPGDSIIFAFTDSLDMHVGAYTLKAWANVWLDNDANNDNINST
                     IDVWGYPTFSLRDTMGLIKDTVKVTNIEYTLNAGIWNSYYWHNDGSTNQFFNQTQTGW
                     AIVTVNDEHSCPATDSLFVDLRFSDITVDTVLVPQTACEISGTVFPKIIIRNAGTDTI
                     TTGTDINLSYKLNDVLQDSPTLTLDSEWYPNETKEIIFGNGVDMTAVGTYNFEFVAKT
                     ASDNKPENDTLRQTVYIYGYPSLELGDSVFTRNNSYLLDAGAGRDSYLWNTAETTQTI
                     TVSQTGKYKVTVTENGFCSTTDSVYVTFLKHDYSITQVVNPTTSCSTPEKQTVSIKYT
                     NMGNDTLKAGQQVRFGYQINESYFAEETYTLLSDLHPGQFLLYNFTEKIDLSEPLTRD
                     MIAYGIYTGDTNTGNDTLLTSFTINESPVVNLGPDRVDRTGSVTLDGGEGTGYTYLWQ
                     DNSTDRYYFAENTGTYWVETTAPNGCSDRDTVNITILRPDYRVTTIIAPSTSCTLSAT
                     EQVQVEVENAGTDTLQIGQNLFVSYEVDNILQQTEAITMTSKFKPGDKLTHSFTKAFN
                     FSTPSTYTIKAYTTSNDDVNPFNNSSSKTIDVWGLPNVNLGIDTAICQGTTHTLDAGA
                     GFSSYLWSTSATTQTIDVSTAGLYWSEVTDSHGCINRDTINIGINPLPTVTHDPLDAV
                     CFDEPEFALSGGNPSGGIYSGPGTNTTTFNASNAGAGTHTLTYTYTDANGCSNSTNVD
                     ITVHPLPVVDLGSNRSTTEPLTLDAGSGFVTYLWQDNSTNQTFTVTETGLFSVTVTDN
                     NGCHGYDEVYITYLETLDIIVSSLLSPADKCYDNKTDSVKVELLNRGTKTFTTGETID
                     VNYYIGTSTPVKEQYTFTSNLPQNGTVQFKFSTPIALSTGEFNFTCFTTIAGDNGDST
                     EFTVNVWELPNFDFGSDTIKHSLPYELESGIGGVTYLWNTGATSPTITISNGQWGKYW
                     LRVTDSHGCVSSDTVVVWWPVSAETIIDTPYRVSIYPNPANDELYIQVDGDRTSNYKI
                     EFIAPSGLTVEQVEIIQSSSFTQRFNVNRLQAGMYFIRISNSQGDTLIKKVIIGRK"
     assembly_gap    29566..29665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29666..30564)
                     /locus_tag="PLW22_02300"
     CDS             complement(<29666..30564)
                     /locus_tag="PLW22_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73565.1"
                     /translation="MKVKELILNTTLCILTISYAKAQTYLSEGFENGTKPTGWTEEYV
                     SGTEPWRYRNGGHSPNDNNWTVPAWQTDITRNPPSAHSGTYNAIFFKQSTNQERTKLI
                     TKAIDLAGAIQPELSFWLCQVPWTFSGNTNWDYLRVYYKKNISDNWTLLQEFLDPIPD
                     WTEFKINLPNPTSTYYIAFEGQTNWGFGTCIDDVQVIERGTQPRYVSELTVVHPDLTF
                     IPSGSKKEPILRLGFKIFGNTGTAALKTIVVKSKNTNDNDILTNGVKLYFTTTAILDT
                     LTPLSTERSFVSGLATFNNINHNL"
     gene            30798..>32644
                     /locus_tag="PLW22_02305"
     CDS             30798..>32644
                     /locus_tag="PLW22_02305"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOU73566.1"
                     /translation="MLKHTIKYWILFLYLQFVAVCAHAQTDTEFWFVVPEVTLSHYAP
                     GGANAYFKFSTGLLPATITISMPANQYDPVTNPTGFPDIVINMAANSFHEEDLSCWIV
                     RRVNPSVPDCNSIPIDTSYALADYADVNRLENKPLNASGINKFGIRITSTNPITAYWE
                     ISRRNNKDIWALKGRNALGLEFFAPFQTHGNNTSILDTPSAIDVVATEDNTIVTFQLP
                     PGIEASYGNPMTNVPAGGTLTRTLNRGETFSLFPRAKNKTIAFKLRGTRVTANKPIAV
                     TLKDDSFYHTSGGCYDIAGDQMVPTSIAGKEYAIIRTFLNNHDHIYILGTQNATSVTV
                     YNTAGGVVASTAINAQQQLYYQIPTTETYYRIVADKPVYVWHVGGFGCEQGGAILPPI
                     DICTGSTQVAFARTSAENFYIILMVRQGAETGFLFDGFVRNDLINPASFIPIPGSQWS
                     VARFGSFAPDSLHILVGSHFIENTEDLFHIGIVNGGSSSGCFYGYFSDYNELDVQAVV
                     AGTNSDVLKTCYGNPVQLYAYGGTDYVWTPDSTLSDPNSQLPWATPKVNTKYKVVVSG
                     ACDMTDSAFIDVLVSTPLTASFITDRVEGCAPLTVNFEDKSTGITYWRY"
     assembly_gap    32645..32744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32745..>35317
                     /locus_tag="PLW22_02310"
     CDS             <32745..>35317
                     /locus_tag="PLW22_02310"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOU73567.1"
                     /translation="VQGCNPQTVTFTNSSSSNTADTYLWEFGDGMNQVTNNPTGTVVH
                     TFTNTVPRDTTYSMRMIATSPFMCRDTATASITIYSFFTANFTTNISSGCSPVTVNID
                     NLSVGDTARYVWTLNGVPLTKTTGCDTTLVLTNTTNTHIDYVIQLQVFNTGNRCSLPV
                     SRTVRVYPEVNAAFTTPQAQYCNLSSVNFNNSTSPASTNPGNVVNTYSWDFGDGAASA
                     LVNPTHVYDNQTNSPKDYVVTLTARNQFGCQDVATTTIRIFSQIYADFAVTPAAQCSP
                     VTASIDNNSRGGIVTYSWDYGDGTPTSGTNLDHTHSYINNELTLIHRTITLTVTNEGN
                     CTDTDSLDVVIYPSVTAGFTPSVTAGCNELTVNFTDNSKANATIYNWDFGDGSSSTQK
                     SPSHTFVNLNGVDETYPVTLTVYTDYGCTAQASTNITVYPFIDAQFAIDTSFGCSPLD
                     VRYVYTKHPGITEYRWDWDDNGTVDQTTLPAAPNTITHQFVNQTGVVQNPTTRLTVVN
                     SHGCSKTTSSTISVYPEVSAIFVPTVDRGCNPLNVTLNNNSIFTGVGTPLVNTNYYWT
                     FGDGGSSTTKSPNHIYINNDPNNNAIYNTRLRIVSEYGCADSAFHIIKVYNRVESHFT
                     LEYAENCTPFDVTFNPAAIGASTYIWTYGGAPGLPASETFANGNPFTRTFTNIDPNNI
                     ATYTVTLEARNNEGCPAFDSHSIEVYPIVDAQFNASTVVGCSDLDVTLTSTSTGGSLV
                     YLWDLGNGQSASTPNVSHTFTNRGSIDSVYAVKLKTINPLGCRDSITRDITVHPKVEA
                     QFVFAQQSPCTPFYIDLTNTSLNGNTFHWYTVYNGDTITNNKNTFQYLFDNSTLNDIL
                     T"
     assembly_gap    35318..35417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35418..>37134
                     /locus_tag="PLW22_02315"
     CDS             <35418..>37134
                     /locus_tag="PLW22_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOU73568.1"
                     /translation="ITVYPLVKADFQWNLFEGCTPLTINLNNSSTSPLYKYRWDFDNG
                     MPYFYSDQPGSVTYTNPTNSPPVILSPTITLRTSYSGDTLCIDSLKLPIRVFPHIYPE
                     FSGNLAGCNPHTVNFTNQTVAYSTNNSYYWDLGSGVNSLLANPTLTYYNTDKFVDSTF
                     TVKMVAVSEHGCTDSVFHQVVAHPRPYAAMELTGEYISCPPFDVEIENNSLGTNLTFE
                     YDFGDGADSTTTSQANMRHIFHNLSNQTQPYQIKLRTVTEYGCDDSVFQTIYVFPEVV
                     ASYVANPGYAACSPFEVNFTNNTINARFYEWDFADGITSSFPTPRHSFLNFGETDSVF
                     NVVLYASSEFGCFDRDTQQIIVYATPIANIAAEPPLQVFPNATFNIYNQSSPAASSWN
                     YNWAFGDNTFSTSKDPLTKTYTRWGPKEQDFRYRITMRVDAPHGCWDADTVLVYLLPT
                     EPEAFFTSSVDSACAPMEVQLVDASQFADSYLWEFGDGATSTERNPRHTYTEPGYYTV
                     KLTVTNDAGSRFYYKIFRVYESPEASFAIFPQRVMLPDATIHAYNLSTQFTRCQWDFG
                     DGYQTT"
BASE COUNT         9435 a         8121 c         8119 g        11059 t
ORIGIN      
        1 aagcgaaaca tctcatgggg ccgattagag atccttcgct ccccgctagg gcgggacagg
       61 ccgctcagga tgacaagcat gtttatgttt gttaactaaa cgacattgaa aagtaattta
      121 cgaatttatt acctacggct ttccttcaat accgagtaga ggctttggta aagcgagttt
      181 gagtcaatgg gtttggttat aaactggttg cagcctaagg ctatgcttat agccctctca
      241 ttgctgaagg tgtgtgcagt ttgcacaatt attggcacct gggcgttggt ggagcgtatc
      301 cttttaatgg catcgtagcc attcatcacc ggcattttga tatccataag tatggcggct
      361 atctcgggat gctgatactg gatctcagtt gctagtagac cattatcagc ctcaagtatg
      421 ttaatgtcaa agtccttgag aactgttttt aggtagtaca tgcagttggg gtcgtcctcg
      481 gcaataagaa tggatttacc tttgagtaga tttttcagtt cctggagttc gtacccgtaa
      541 acctttcccc tattttgggt tgtaggagca ttaataggta atgtgaaacg gaaagtggag
      601 cccttacccg tttccgattc aacccatatt ctgccgccca taagctcaac caatgatttg
      661 cagatagcca gtcctaagcc cgtgccaccg tactgcttgg ttatatcggt ataggcctga
      721 cggaaacgct ggaaaataat ttcctgcttg tccttggcaa tacctatacc ggtatcgctt
      781 acaaagaact caaactcatt ggtttcgcta tttatgttgt agccaaattc cactttcccc
      841 ttatcggtga acttaagggc gttgtttata aggtttgtaa gcacctgacg taatcgcatg
      901 cggtcggcat taaccatggc cttggagtcg ggcagtgagg tggtgtagtt aaaagctatt
      961 tcacgcttgg gtaagtcggg gtttgcgttg tagaagaaag tgtatatctc gttaagcagc
     1021 tcgttaatgt taaccgcttc ggggttaatg gttagctgac caacgtcaat ctttgagata
     1081 tcaataatat cgttgattag ggtaaggaga tgatcggcgc tggtgctaat tattcccagg
     1141 aatttctcgc gctcttggag cgtggtatca gggaagttgc ggagaaactt ggcaaagcca
     1201 ataacaccgt ttagcggtgt gcgaatttcg tggctcatgt tagccagaaa tgctgttttt
     1261 agcctgtcgc tctcctcagc cttttccaat gcctcaacaa gtgccttctc gtattgggta
     1321 cgttcgctaa tgtcgtttgc aatacccaaa acaccaatgg tttcacctgt tgttgacgtt
     1381 agcttttgct tgaatatttg aagaatgtgc tttttgccat cggtaaaagt aacctcctcg
     1441 gtggtttcaa ttggcccatc ggagtttagt gcctgcatat cgctacggcg gcattgggca
     1501 acctcctcct cggtcatgga gaattgcgat gtgccatagc ctatgttttg gctaaaccat
     1561 ggatttatta ttgggtatga gccgtcgggg ttaacaaccc aaatgccaag cggggcattg
     1621 tctactattg attttaatag gttgcgctgt tgctgaagtt cctgctcctg ttctttttta
     1681 gcggtaatgt cctttactgc gctaatcagt cggaaaggtt ttccgctgct atcgaattca
     1741 gggtaaacag tattctcaat ccagtgtact ttgccatttt tggcctttat tctaaactct
     1801 aaagtaactt tctcgccttt ctgggtgata gtcccaatgg ctctcttgaa aacatcaatg
     1861 tcgtcggggt ggacaatggt tgcccatttt tcaaatgaat caacatcctc gggcttatag
     1921 cctagctgaa ccattagtgc ggagctaagc cactcacgtt taaatgagcc gtcgggttga
     1981 atggtgagta ttgaagccga atcggaagtg agcgatgata ggattctgaa acgttcctcc
     2041 ttgatggtta gctcctcctg tgttcggtaa agatcggaaa tatcctctac aattcccatt
     2101 gctcccacaa cctgtccggt tttagggttg gttaatggca ctgctttcag taaaacgtaa
     2161 atctgagcgt tgctggttgt ggttgtgtat ggcccctcgt agtatccgtt ttttccatca
     2221 agtgcatcga gtatggccgg taatactgac ttgtcgttaa ttttatgcat gttcaaacca
     2281 accagcagct cgcgctttga ttttagtatc tcaacaaaac ggtcgttgca ttcggtgata
     2341 acagcatcgg tgttatactg aaataaacca ataggcgatt tttcaaatat cagcctatac
     2401 ttaagttcgc catcgtattc aaagctacct tcaaacctct tttcgcccac ttttttttga
     2461 gccataggta tagtagattt tttcgtaaga tacgactttt acggcggtaa tccaaaaact
     2521 ttttaagggg tgctgattca ctgttagttt agttggtaaa aatcactcct ttgtcatcct
     2581 gagcaaccta caacgccttg gcggggagcg aaggatctct ctgtaccgct ttatttgaga
     2641 tgtttcgatt agctcaacat gacaagcatt cctagctaga cgatttgggt gtagagggct
     2701 gaaaatacca ttaagcaagt aatgaatatt ttcctgcgct ctgctcagga taataagtgt
     2761 aagcaatgct aacaatgact aaaatacggc ttttgcaatt tgtgcaatat tatcgtcctt
     2821 acccatggtg tagaaatgga tggcttttac tcccgaactc tttagttccc ttgattgttc
     2881 aattgcccat tccactccaa gctgcgctac ctggttgttt gtcttacatt tttccagttc
     2941 aagaaccagc gcttgaggta tatgaacatc gaacaccctg ggaagaatgc taagatgctc
     3001 ctttatgctg atgggcttca acccgggaac gatggggaca gctattccgg ccttacggca
     3061 tttatcaaca aagttgaagt aaagcttgtt gtcgaaaaac atttgggtaa caatgtagct
     3121 tgctccggct tcaacctttt tctttaggta gtgaatatcc gactcgcagt ttggcgattc
     3181 agggtgtttt tcagggtacc cagccacccc tatgcaaaag ttagttggtt tgggtttttc
     3241 catcagcttt tcaaggtaaa cgccacggtt caggtcagta acctgtttca ccaggtcaat
     3301 ggcataccta tgtcctcctg ccctgggttc aaacctgccc gtaatcttat cggcatcgcc
     3361 acgcactgcg agtatgtttt gtattcccaa aaaatcgagg tcaataaggg catcctctgt
     3421 ctcgtgggcg tcaaatccgc cgcagataat atggggcaca acgtcaattg cgtacctctg
     3481 ctgtattgca gcggcaagtc caatggtgcc ggggcgccgc ctaaccttta caggaacctg
     3541 tatcccgttt tcgttggaca tgtaaaccac ctcctcgcgg tggtaggtta tattgatata
     3601 tgtcgggttg tagggcaaaa gtttgtcgat tgtggcaaaa atggtttcaa tgttatgccc
     3661 cttgagcggt ggtaaaagtt caaaggaaaa aagtgtcgac tttgaattat taagtatatc
     3721 gattaccttc atctcagtaa attttattgg tttgctaaaa aggttggaaa caaccgagct
     3781 gcttcggttg tactaacacc aattcgtttg gcatagtccg ccagctggtc gttgtcaatt
     3841 ttaccaacgc tgaaatactt gcttgcacta ctggcaaagt agaagcctgc cacggaggcc
     3901 aatgggttca tggccatatt ctcagtaagg cttatggaag cattccgtgg aatatcgaga
     3961 agctgaaaaa tccattgctt accacgatga tcggggcaag ccgggtaacc cggagcaggc
     4021 cttatgccta catatttttc ctgcagcagc tgttccacgg ttagcgactc gttgggtgag
     4081 tagccccaga agtcctttcg gatttttagg tgtagccatt cagcaaaagc ttcggcaagc
     4141 ctgtcggcca aaatttgaat cattatggcg gcaaagtcgt ttccattttt cctgtactcc
     4201 tcaataagtg ttgatgtggt ggagccagta gtaaccgcaa aacatcctat ccaatcgtta
     4261 atgcctgaat ttgcgggcgc aataaagtca gaaaggcaca tatttggctc accctctggc
     4321 ttttgctcct ggtttcgcag gaacggcaaa tgggttaggg gcgatgttcg ggtttcgtcg
     4381 gtgtacacaa taacatcgtc gccactggaa gcggctggga atattccgta aaccccttcg
     4441 ggggttaaaa gcttctgttc actgatttgc ttgagcattg ccagggcttc cgctttaagt
     4501 ttttgtgctt cgctgccatg aacaggatcg gtaagtatat cggggtaacg gccttttaaa
     4561 ccccatgccc tgaaaaagaa tgtccaatcg atatagtgta ttagctcatt taaatcgata
     4621 ttgcttaagg tattgatgcc tatttgcttt ggcctaactg gtttgtagct atcccagttc
     4681 agcttaaggc tatttttgcg ggcttgctca attggaacta aaacagtttt actccctgaa
     4741 tagctttcgc gttgttgctg gtaacgcttt tggtaggcat ttatggtact tgcggacttg
     4801 tcagtgctca gcaagctggc aacaataccg cttgcctgtg aggcatccct aacatggaca
     4861 actgctcccg agtattcagg tgcaattttc agggcagtat gcagttccga tgtggtagca
     4921 ccaccaatga gaaccggaat gttaatgccg tttcgtttta gttcctttac cacatgtacc
     4981 atctcgtcga gcgagggagt gataaggccg ctaaggccaa caatatcggc tttacttgct
     5041 tttacttcat ccacaattct ctcggctggt accattaccc ccaaatcgat gatgctgtaa
     5101 ccgttgcagg cgagaaccac accaacaata ttcttaccaa tatcgtgaac atcgcctttt
     5161 acagttgcca gtactatttt cttttgttct gtcctatgtg aatccattgc ttgtgcttcg
     5221 aggtagggtt ccaaaacctt tacagcctgt ttcattacgc gcgcactttt aacaacctgg
     5281 ggcagaaaca ttcggcctgt accaaaaaga ttgcccacct cgcgcattcc atccatcaat
     5341 ggcccctcaa ttacactgat aggcgatttt agcttgttgt aggcttcctc cacatcaacc
     5401 cgaataaact catcggttcc cttgataagt gagtgcttaa gcctttgctc aacgggtagt
     5461 aaacgccagt cgtcggtttt gtgatgctgc aggtcagttt cttcaagctt actggcatag
     5521 gccaaaagcc tttcggttgc gtcgcgccgg cgatttagca ctaagtcctc agtgagttta
     5581 agtaaatttg gttcaatatc ggtgtaaacc atcagcagcg atgggtttac aatacccata
     5641 tccatacctg cctgaatggc gtggtaaagg aataccgagt gaatggcctc ccttaccagg
     5701 ttgttgcccc tgaacgagaa tgaaaggtta ctaacgccac cgctaacttt ggcgtagggt
     5761 aggttctgtt ttatccattt aatggtttca ataaagctaa cggcttgtgt tgaatgttca
     5821 accatgcctg ttccaatggc aaggatattt gggtcgataa tgatatcctc aggcgaaaag
     5881 ccaacctttt cggttaaaat tcggtagcta cgttcggcaa cgcggcagcg gtgctcggta
     5941 gtaacagcct gcccgctttc gtcgaaaagc atcacaacca ctgcggcgcc ataccgttta
     6001 ataacttttg ccctacgggc aaactccgtt tcaccctctt tcaaacttat tgagtttact
     6061 aagcttttcc cctgagtgca tttcagccct gcctcaatta catcgaaacg cgatgaatca
     6121 atcattacag gaatacgggc aacgtcgggt tccgatgcta tcaggtttag gaattcagtc
     6181 atcgctcttg gagcatcaat tagggcgtcg tccatgcaaa tgtcaatagc ctgggcgcca
     6241 ccctcaacct gttcacgggc aaccgaaagg gcctcggcat acttaccttc ggctatcaag
     6301 cgggcaaact tttttgaccc tgccacattg gttcgctcgc caatgttgat gaaattgctt
     6361 tcgggtctta tctcaagcaa ttccaaaccg cttagtcgag tatggcgtgg aatttctggt
     6421 ataatcctgg gtttatagcg ttgggccagg ttggcaacaa gctcaatgtg cttgggtgtg
     6481 gttccgcaac atcctcctac aatgtttacc agcccattgg ctagcatttg ctcaacaatt
     6541 tcagccattt cctgcgccga ttggtcgtat tccccaaact gatttggtag cccggcattg
     6601 gggtgtgcgc ttacaaaaaa tggtgtaata gccgaaagtt cctcgacaaa cggatacatc
     6661 tgttctgcgc caaaggcgca gttcaagcca atgcttagta gcgggaagtg cgatacagag
     6721 cagacaaatg cctcaagggt ttgcccggta agcaatcgtc ccccgttatc ggctatggtt
     6781 gccgacacca taacaggaat atcgatacct ctgcgttcaa gtatctctga aatggcaaac
     6841 agcgcagctt tggcgttaag ggtatcgaat atggtttcaa ttagcaggat atcaacccca
     6901 ccatcaataa gcgtttcagc ttgctctgtg tatgagttta caagctggtc aaaggttact
     6961 gcacggtagc caggacgctc cacatcgggc gacatgctgg cagtacggtt ggttggccct
     7021 atggaaccgg ctacaaatcg cggtttttgc ggattggcat tactgaactc atcggcaatg
     7081 gaacgggcaa gcctggctcc ggctaggttt agctcgtaaa ccatctgctc caaaccgtag
     7141 tccgattgcg aaatgcgtgt ggagttaaag gtgttggttt caattatgtc ggctccagcc
     7201 tcaaggtagg cacggtgtat ctgccctatc acatcggggc gggtaaggtt aagcaggtcg
     7261 ttgtcgccct taaggtttac cgggtgattc ttgagaatat cgcctctaaa atcctcctcc
     7321 tttagtctgt acctttgaat catggtgccc atggcaccat ccagcaccag taccctgctt
     7381 ttcagtaact ccctaaaaac tgttgttttt tccataaaat caatgttcag aatgcatcaa
     7441 caattggttg gtaatcatca gcacaagtta ccttgcttga tagtgccttg tcaatagggt
     7501 taaacctaaa gttaccggta aaagcaagaa acaggtttaa ctttggaagc aactccttta
     7561 ctctgataag ttcattgagt tttacccatg ctatttccca tcggtaatcg gtggcgaaat
     7621 gttgttctgt aaccttgcgt tccccaaaaa tactgacatc agttaggttg tggtatcgga
     7681 tatagagctc cagctcaacg tctgttgaat aggctggtgg cgtaaagtat ttttgctttt
     7741 tatactcgta acggtagtta tggaagcgag ctgtaatgtc gctcagcaca gccgaaccgg
     7801 ttgggtatcc gcccgcaccc ttaccaaaca tgaattgccg gtcgtagtat tgtccctcaa
     7861 caaccacccc gttaaactca ttctcaacat tatagatata atccgagggt tctaccagtg
     7921 ttgggataac aaaaagtgta aagtagtcgg catttagctt ttgtagctga gcaaccagct
     7981 tgaacttttt ccccttttcg cgggcaaact gtatgtcgta agccttaaga ttgtttatgc
     8041 catagcaaaa ggcatcgcta gggtgaacgt atgtaccaaa tccatgcacg gcaagtatta
     8101 tcagcttata gagtgcatcg aatcctagca catcaaaatc ggggttgctt tcggcaaaac
     8161 ccagttcctg tgcttgtttt agcgcccaat gataatctac cccttggctg aatacgttgg
     8221 aaaggatata gtttgttgag ccattaagga tgccacgaat cgattttaaa aggtcgttgt
     8281 cgtagtattc ttcaaggttc cgaattacag gtatactccc acatgctgaa gcatcgtaaa
     8341 gcaaagccgc tcccgtttca tgctgtatgg caattagttc cggaagatgg tgtgccagca
     8401 tggttttgtt ccctgaaacc acactcttgc ctctaagtaa cgccgatttt actatgtggt
     8461 atgcttcatc ggcgttatcg ataagttcaa caatcaggtt aatttcctca tcgttaatta
     8521 tatccgcagc attgtaggta attaattcgt gtggaacctc cctgttttta tggcgttgct
     8581 taacacagat gcgctttatg tatgctttgc tctttggaat ttcagcaatt acgtggtaaa
     8641 gcgcattgcc cacattgcca agtccgaaaa gaccaataac ctgttggttt actccgtttg
     8701 actttgagtt tttacgtgta tttgtgattg ttaaagtttc catttctgtt gattttttaa
     8761 aggttgatga attcgctgat aattgatgtt agctgttggt actcaatcag aaatccatcg
     8821 tggcagaact gggagtgaat ttcgcggtaa accgcgcttg ggatgtggtc tgcaatgaaa
     8881 tgttgctcct caactgggaa aagtatatcg ctatcgatac ccacaaccag tgttcgtgct
     8941 tttattgagt ttaacgccgc ttcattgctt accctacctc tgcccatgtt atggctatcg
     9001 agcattaggc tcagggcgaa ataggagtag gcgtcaaacc gcttaaccag tttatcgccc
     9061 tggtagtcct ggtatgtgca ggctttaaag tccgatagct gatttgggtc actatcttgt
     9121 tgggttaggt tgtagctgct gcgtccgcgg tagctaagca gcgcaatgct acgcgctgct
     9181 ttaagccctg ctttacctcc gtttggatta gcaccgtcaa aggttccatc ggcaagtatg
     9241 gcaagccgtt gcgtttggct gaatgcttta acccaaggcg aagcctgggc agcagtggca
     9301 atcagtataa gattctcggt tgtgccggga tacatacatt cccattccat ggcctggaat
     9361 ccgccaattg aaccgccggt taatacccat atcttgtcaa taccaaggta ctctttaagg
     9421 atttggtgcg cagtaaccac atcgcggaag gttactaacg gaaagctcct gaggtaaggt
     9481 ttgcctgttt tagggttaac gctcataggc ccggttgaac catagcatga gccaatgatg
     9541 ttggcgcaca ccacagtgta tttttcaggg tcgaacaacc taccttgtcc aaccatctcg
     9601 ggccaccagt cggccggatt gctgttggcc gtaaaggcat ggcaaatcca aataactctc
     9661 tcgggatttt tttgaatatc gcctagtata tggtaacgaa tgtttacatc gtatagcact
     9721 tcgccacatt caagttggag tgttttaagt tccaggttgc cttccgttac tttcatgtcg
     9781 gtttttttta aaagatttct gccacaatgt attaaattgc tttgagttag ctaaaggttt
     9841 tttgaaaggc ctgttcaaag tcggcaataa tatcgttgat gtgctcaatg ccaaccgaaa
     9901 ccctaagtag gtttggcaac acgcccgctg caaggcgttc ctcttcacta agctgttggt
     9961 gagttgtagc agcgggttgg ataatcaacg ttttggcatc gccaacatta gcaagatggc
    10021 taacaagctt aaggctatcg acaaacgttt gggtttgctc caggttaccc ttgagtgtaa
    10081 atgtcaatac tcccccaaag ccatttttaa ggtatttttt tgcttgaatg tggtttgggt
    10141 catcgggtag tccggggtag caaacccttt caacatgcct gtttgttttg agccacttgg
    10201 caagcgcaag ggcattgctg caatgccttt ccatgcgcag cgataaagtt tcaattcctt
    10261 gtaatatttg aaaagcattg aaagggctga tggctgggcc aaaatcgcgt aacccttcaa
    10321 cccttgccct aatggcaaat gcaatgttcc catgagggct atcctcgcca aatgcagtcc
    10381 agaatactaa cccatggtaa ccctccgatg gctccgaaaa ctgtggaaat tttccgtttc
    10441 cccagttaaa gttgccggca tcaactatga caccacccat gctggttccg tgtcctcctg
    10501 cccatttggt tattgattcc acaacaatgt tagcccccca ttcaatgggt ctgcaaaggt
    10561 aaccagcacc tgcaaaggtg ttatcaacaa ttagtgggat ttggtgttgg tttgccaggg
    10621 ttgtaagtgc cttaaaatcg gggatgttaa agcttgggtt tccaatggtt tcaacgtaaa
    10681 tggctttggt tttactatca atcaagcatt caaaatcttc cggtttaagg ctttttggaa
    10741 gccttgcctc aatgcccagg cgtttaaggg tgaccttaaa ctggttatgg cttccgccat
    10801 acaggtaggg agagcttacg aaattatcgc ctgactccat aatgttggta agcgctataa
    10861 actgcgcagc atgccccgac gataccacca cagccgattt gcctccctct agcgcagcaa
    10921 gccgttgctc aagtacatcg gttgtaggat tgcttaaccg tgtgtaaata tgcccaaact
    10981 cctttaaggc aaaaaggttt gccccgtgct ctgaatcccg gaaaaggtac gatgtggttt
    11041 gatatatagg tacagcccgc gaaagcgtgt ttgtatccac cccttgccct gcatgaacct
    11101 gcagggtgtc aaaatgatat gtattgttca ttgtctcaat atttttattg gaaatggtaa
    11161 agtgtgagat ttttgcctat atacaggcaa ttgtttagcc cgccgcaaac ggcatgcagg
    11221 ctaaaaccaa taggttttat gaattagcta ttgcgcgcag gggcgcattc gcattgaaag
    11281 agagtgggaa agcctaaaaa gtaaattgtt tagcgcaaaa gaactgattg cgcataattc
    11341 aatactgttg agatgtttac tttgcataac atcagagttt agagttataa ttaccctctc
    11401 gggtcaggtt ttagcacctt gccttttggg gggcaggttg ctagagcttc attgagcctg
    11461 ttctctccgc tcttctctat aattcaaacc cttctttatc taaaaagaat ggcattcgat
    11521 ggcaacaaat atataaaaaa ttctatttat gaaatttatg gcggtaaaac ttatattaaa
    11581 ttttgaattg attaaatgtg gtaaactatt aatagtaggt tgacatcagg aatgggataa
    11641 agttactttt tcgaaccctt tgtcctaata aaaatcatag caatagcagt ggttaaaatt
    11701 cccattacca atgcaccaat agcactctga atagcaaaat ttttgtagtt gaaaaaggct
    11761 tcagcttcag caacagactt gtagtaaccc aattccacag aacgttttat aacatttggg
    11821 aaatactcgg gtgaaatcac atacgatatt atccactggg ttaaggggct taaaatcgca
    11881 atgaataggg agagcacaat accagtaatt aagccattca ggtagctcat gttgccatga
    11941 aagaaatttt gttttttatc ctttaatgcc agaaccatta cagctatagc tgggatagca
    12001 aaaagatttg tgaggtaata ctggtaatca atatatttcc cgtgtaaccc cgataccttt
    12061 tcaagcacca tccaagccaa ggtcattagc gaaaatatta atgcccattt ggcttcaatt
    12121 cggaatctac ccatgttttt tctagattgt ggtttgtttg aacaagttct ttgagtaaac
    12181 ccggcaagaa tcaaagtatt ccattatggc cttgtctttt ggaaggcagt ggaatcgtta
    12241 agactaatct tttcgtactt tggttttacc acttcccggc cttcgctcga aataaaaccc
    12301 caaaggccat cgcgttccac aagagcccaa cccctttgca cctggtcaaa aggcatgatc
    12361 tgagtgtact gtggtttaat gacttccttg ccctcggagt taataaatcc catgtagttg
    12421 tcgcgctgta ctaacgccca acccttttgg tactcgttaa actgactgat ttttgagtat
    12481 tgtggtttaa ctatttctct cccttcgcca ttgataaagc caagaaaacc atccttttca
    12541 accaatgccc aatccttttg gtattcgcca aacggatgca ccttctggta ctggggtttt
    12601 agaacttccc ttccatcgag cgatataaag ccaattaatc catctttctc aaccatggct
    12661 aggtttttgt agaactcgcc gatagggtaa attttactat aaattggctt gataatttca
    12721 cgaccttcaa ccgatatgaa tccaaactta ccgttcacct ctaccattgc ccatccgcgg
    12781 ttaaagtcac caaaggggta tatttttgtg tattggggtt tgataatctc tttgccctcg
    12841 gccgagataa atccgaaaaa tccatccttt tcaaccattg cccacccccg ctgaaattca
    12901 tcgaaaggat gaatacgtgt gtattgtggc ttaataatct ccttgccggt gctggaaata
    12961 aatccaaatg agccaccctt ttccaccaaa acccagtcgt tggggtatgg ccctttaggt
    13021 tgccgctttt tacccccggc ttgctgccca tgtacaaaac taaacgccaa aacagcaatt
    13081 agtgcgagta gatatttttg catagccaac atattaatgt tagggcacaa gtgtaaatac
    13141 ttgcttcact atcatacaaa cctacatgat ttttcttaaa attcatatag gttgataaaa
    13201 aagtaggcaa agtataagca ttttgcgttt gaaatcagta aacttcgaaa atcaatttcc
    13261 atcacaatct attaaaaagg tagatttaag atttaaaatt taagaactca cttgttaata
    13321 cacgtctttc gacacgcata ttattccgcc ctaaaaggat aaatcacatt agaccagggt
    13381 tttaacccag ggtattgtgg taatcaaaat tttaggcgat gcacgcaatg ctttttccaa
    13441 agagcaacgc tgaattgttg catcgcaaca gaagaacatt ccaaagagca aaaagtaaat
    13501 aagagaatga aggcacaaag agatgcttcg gcgagctcag cttgacaata gtttcacgaa
    13561 caacgaacac cgtttccagc cctgtagggg cgtaatattt gtaacaccat ggctaatccc
    13621 tcaagtatag cgatgcacgc tatgatacta caaagagcaa tattgaaacg ttgcatcgca
    13681 actgaaatgc atggcaaaga gcgatttaca tgttcttgct tgtcttgata caagaaagaa
    13741 ccaaagaaga tcaaggctga aaagcgcgaa ccgatgggtg ccccgggggt ggaacttcca
    13801 cgcgatacta ttcgccacgc ctgcggcgtg actcatgtgg tatcgcttac taagcccacc
    13861 gccacgttcc acccccgacc cattgctgag tcaggctttt atgccactac ttgcttcttc
    13921 cgctacattc cacctcaggg aatagggctg ggggagaagc cgttttctac atttaaccta
    13981 gacgccccct ctaaatctcc cctgaaaggg agactttagc cttgcaataa ggtttcctaa
    14041 ttgcatcact cctctccttt caggagaggg gagggggtga ggtcaatttc acgaacaacg
    14101 agcaactaac aacgaacaac gtcttaaacc ccgtaggggc gtaatttagt ttttactgaa
    14161 ataacaaatc ttcaatgtct tactttgagg ctttgaactt aggaactatt ctgtgctttg
    14221 ttccttgctc gtaggagtat gctgcttcaa taagtgttga ctcacaccag gcgcgcccaa
    14281 taaacgagat accaacaggc aaaccatcaa tattccccat tggaacggta atgcttgggt
    14341 aaccagccat agctgcatac gaagtgttac cacctatgta attatcacct aaaatcaaat
    14401 cggttttcca tgcaggcgaa cctgttggaa ttatgagtag gtcaagtttg ttttcgtcca
    14461 tcactttatc aattccattc tgccttatac ctttttgcat ggtttcaagt gcctttatat
    14521 attcaggcga gtttaggtca cctttgcctt gagcaagttc aagcagatgt tggtcaaagt
    14581 ggcgtagttc aatagtgtcc gtacggttaa aagaaattaa ctcctctaag ctcttaattg
    14641 gtgctttttc gcctaaaccg gcaaagtagc ggttaagccc atccttaaac tcatagagca
    14701 atacctgaaa ttctgcactg tttacttctt gaggtatggg caggctaacc tcaaacacct
    14761 cggctccaaa acggcgcaaa tcgcttatgg ccttgctcat gagcgtgtca accttatggt
    14821 gaaagcccga tgcattggta ataaatccaa tacgttttcc cattagcccg tcaagtcgga
    14881 ggaactgtgt gtagtcgtta taaaacctgg cgttagggtc aagtgttttt gggtcgtttg
    14941 gatcggggcc aaccattgct ccaaggcagt aggccacatc ggaaaccgaa cggcacatgg
    15001 ggcctggtgt gtcctgggtg aacgagatgg gaattatccc gctgcggcta ataagcccaa
    15061 ctgtgggctt taggcccaca atcccgttgt tgttcgacgg gcacatgatt gaaccatcgg
    15121 tttcagtgcc aatggcaaat gcacaaaggt tagccgatac ggccacgccc gaccccgaac
    15181 tcgacccaca ggggtttctg tcaagttcgt atgggtttcg ggtttgccca ccaactccgc
    15241 tccatccgct cgacgaacga ctcgccctaa agttagccca ttcgctcagg tttgctttgg
    15301 ctattattac tgcaccggct tcgcgaagtt ttgcagcaat ccagctatcg cgtccaacga
    15361 aggagttcct taaaaccgtt gcaccggcag tggttggcat ggagtcgcgt gtatcaatgt
    15421 tatcctttag gattacaggt attccatgaa gtgggccacg aaccttgcct tgtgctaatt
    15481 ccttatctaa ttccctggct atttttagcg catcggggtt gatggctata accgagttca
    15541 ggcaaggacc ttctttatca aggagttcaa tgcgcttcag atagtcgttt acaaccttct
    15601 ccacggtaaa gcgcttttcg cgataaccgt cctgcagctg ttttatggta atttcttcta
    15661 ggtatgcagt gctgtcaata tctgacgagc cttgcctttg gcacgataca agtgtcgctg
    15721 caacagctat tattaatatg attttgattt tcatggtatt ttgtttttgg caatcgaaat
    15781 ttagtgaaaa aaataaaggc agtcaattag ggactgcctt tattgtaata ctgtaatagg
    15841 aactacttac tgaagagtgt tttttcgctg atttttacta gtttgccgta tttggcaagg
    15901 ctcttcatgt cgatattctt ttcatcgcca acaatcatgt aaacaacagg gttgtttttc
    15961 atgcgatcct tgtagaattc atcaatattt tcccatgtta aaccccggta aattggtagt
    16021 ttaataatta gtggatcaac cggaaagcct ctttttctcc acacatcaac agttgatgca
    16081 atattgcgga agtttggtcg gctggtttgt gccgatagct ccaggtggct tttaatcatc
    16141 tcaatacgtt caggttttgc tggcatttgc ctgataagag tatcgagggt ttccatggca
    16201 ataagcgttt tatcggactg ggttccaaca taaccaataa agttaaccgg tttacccttt
    16261 tctagcgggt agcggaacga gccacctgta gcgtatgcca gtgagcgata ttcgcgtatt
    16321 tcctgaagca ttaagccgga aaaacctccg ccaaagtagt cgttaaaggc atcgatgtat
    16381 gcagcatcac caatttcaaa gggtttgcca ttaataaata caaacatctt gctttgacgt
    16441 gcctttggtt tattgacaaa aaggatggtg ttttcggtgt actgtttagc aggtttatcg
    16501 aggggtgttg tgtcaaccac tgggcttgag gagagtggta agtagctggc tattatatcg
    16561 gaaattttat cggcactggt attaccgcaa tagcttatgg tggcagtata accagccgct
    16621 tgcttaaaag catcaaccag ctcttgggct ttaagctttt ttacactgct catggataac
    16681 cgtttggtat agtaggattc atcgccatat aacccatatt caactagtgc atcggccacg
    16741 ttgtcgggtt cggagcgttc catcttacgc gttgccttct cgttggccac aattgtttta
    16801 atttttgatt gttcaagttt ggggtcgcta agcagtaagc caacaagttt gagtgtgggt
    16861 tccaggttgc tttcaatacc attaatggtt atagtagttt tattttcatt agcccagatg
    16921 ctatacgatg agcctaactt ggcaaattca gttttcagtg cgtttacatc gtattcgcct
    16981 gctccggcta aagccatacc ctgtgtagca aatccaagca atggcagtga agctttacct
    17041 actttaaact caatactcaa tgagaaaata tcgttcttgg ggttttcaac gcattggagc
    17101 ctatgcccac cggcaagttc aaccactttc aaatcttttt caaaatcaat gaatttaaag
    17161 ttaggggtgc gaatagggag cgagtcgaaa tgctgggcat aagcgcttcg ggcttttgta
    17221 tttgagagta ggggcttgaa gtcgggcttg gctatctttt cctttttggg gaagcccatt
    17281 ttggagtaaa aggcaaggta gttagggcca aaaacctcgt ttgcaatagc tacaatatcg
    17341 gctttggtta tcgattttac cttttcgggg taggtgagtg cctcatcaat acttttacct
    17401 tgggtgaagg cttcgctgag ggttaatgct ctgttctgaa tgttttcgag ttttgtgttt
    17461 gtgttacggt aaatttcaag ctttattgct tccaacaggc tctcgtcaaa ttctcctttt
    17521 ttaagctttt caatttcaga gagaaccaat ttttcggcat cttcaagttt ttggccaacc
    17581 accttgggaa caacaataaa gattattgct ccatggtcgt agtatggcat catcatggct
    17641 tgagctgcaa gcaattttcc atcgagcgtt agcttgtcca gtaaaccggt agagtagctg
    17701 ttgttaagta atcgggtagt aacatcgagc aagggcttac ggggatcggt agcgtttgga
    17761 gcgcgataac caagtattgc aagctttata ggactaagtt tcgcctcgtg gaattcgcgt
    17821 ccgttaaagg gagcctcatc ccattttcga ggttcaggca gttcacgggg ctgccatttg
    17881 ccaaactttt cttcaatggc gggaataact tcttcggaat taaaatcgcc cgataggatt
    17941 agggccatgt tgttgggcac atagtatgtt ttgaaaaact catacatttt ggtaagcgaa
    18001 ggattcttta agtcctcaat gcttcctatc agggtttgtt gtccataagg gtgatttttg
    18061 aaaaaatatt tctggaattc ctctagtaaa cgggtctgga actgatcgtt gtaaaggttt
    18121 ttctcctcat aaaccacttc gagttctgcc tgaaaacctc taaatacggg ttcagtgaac
    18181 cgatgggcgt ataggtcgag ccaacgttcc acctggcttg ccggaaattt gttgtagtat
    18241 agggtgtaat caggacctgt tccggcatta attagggttg aacccatttc attcagcagg
    18301 ttattcatct cgttgggtat ggcgaatttg tttgcctcaa cggactcttc gtttatcgct
    18361 ttctgaatta atgctctttt ttcgggatct ttggttttgc caagcgaatc gtaaagctga
    18421 aatatcctgt cgatatgtgg tttctccttt tcccagtcaa tggttcccag cgtggttgtc
    18481 cccttaaaaa gcatatgttc ctggtagtgg gccatgccgg tagcatcggc ggggtcgttc
    18541 ttgccgccag ttttcactgc aatcaggcca aacacttcgg gttggtcgtg ttgttcatca
    18601 agtataacgg ttaatccgtt gggcaaggta aattgcctga ttgtttgggt ttgagcaaat
    18661 gactgtaacg caatggcaat aagtgcaaat gcaataattt tttttaaaaa tttcatagtt
    18721 ttaaatggtt tacgtttatt ggttagccta tggattttgt tatttgtaaa gataaaaaaa
    18781 tcttacttag ctgactgact gtttaagatt ttgcttttta acggcgttat tattccccca
    18841 aaaaaacaaa accggaaaag ttcagagatg aactattaat ccggttttgc ttaaaataag
    18901 ttatctaggt tttaatgttt ctattgtcac acaaaagaca tccttttacc ctttatcctt
    18961 tatcctttat ccttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccttta
    19081 tccttatcta tcttactcaa tcacggttac ccatccatgc ttatcttcaa gctcgccgta
    19141 ttgaattcca acaagggttt cgtaaagttt ggttgagatt tggccgggct taccatcctt
    19201 gcagaatgtg taaaccttcc ctgtttccaa atccttgatc tccccaattg gtgaaatgat
    19261 tgcagcggtt ccgcatgctc caacctcttc aaaagtttca agttcctcaa caggaacagg
    19321 gcggcgttca accttcaagc ccatatcctc ggcaagctga ataatgctct tgttagtaat
    19381 ggagcctagt attgatgttg attttggggt gatgtaggtg ttgttcttga taccaaagaa
    19441 attggcagca cctatttcat caatatactt cttctccttt gcatcaagat acattggtga
    19501 actgtatcct gctttgcggg ctttctctac cccccgaaga ctggctgcgt aatttccacc
    19561 taccttaaag gtaccagtac caagtggggc tgctcggtcg ctatcgcgaa caatggcaat
    19621 tttaacaggg ttgaagcctt ccttaaagta tggtcctaca ggagtaacaa acacaacaaa
    19681 catgtactcc ttagcaggcc gaacgccaac ctcggcacca gagccaaata gcaaaggcct
    19741 aatgtaaagc gatgccccag ttccgaatgg cggtacaaac tcctcgttca gctttacggc
    19801 tttaaccacg gcttcgtaaa aaagttcttt gggcacaggt gccattaaaa tcccttcagc
    19861 tgagtgttga agccgctggg cgttctcatc ccatctgaaa agacgaattt tcccatcttt
    19921 gccacggtaa gccttcatgc cctcaaaggc ctcttggccg taatgcaggg ccgtagcggc
    19981 catgtgaatg ttaatatatt ctgaatccga tacctcaagg ctgctccatt tgccatcctt
    20041 gtaatagcat cgaacgttat acttggtttt aacgtaacca aacggaagat tcttccagtc
    20101 taaattttgc atgattccaa cagtttaatg aattggacat attgtgatat gacaaagtta
    20161 aagcaaatct gttttaatcg cttcatattt ttagatattt tacaataaaa aggtttaaaa
    20221 tgtaagtttg actcgtaaaa cggtaataat ttgaaagata catttcggtt atgtaaaaaa
    20281 aacgcacgca atcattcagc gcgcgttaat ttgggtagga gtgtattagg aataattgaa
    20341 accagcaatg cctgtttttt ataacaaaat catgtgtgtt attttttagg atatgactcg
    20401 gaatgacaag tagcgttgtt cgtgattcgt tattcgtaaa aagaaccttt tgtattaacc
    20461 tcttctaaat tttatccatt atccttcttg ataacagggg gtttcgctta ttgttcaatc
    20521 ccatttaacg gctcacgttt aacggttcac gctttaccct ttatccttta tcctttatcc
    20581 tttatccttc gtcataatcc ttgcccctcc ggccaaaccg atgcttgcac ctgtgcaata
    20641 tgtaccttgc ggagtaagaa catgcagagt cgcgattgcc ctatgcctcc cccaatgctt
    20701 tgtggaagtt taccgtttag tagcaagcta tggtagtgca atttacttcg atgctctaaa
    20761 ccttgcatct gcagctgtaa ctttagggct gcactatcca ccctaattcc catcgatgag
    20821 agttcaaggg caattccaag tggcttgtgc caaacaagaa tatcgccatt tagacctctg
    20881 tatccatcgg ggcggagtgt tgaccaatca tcgtaatcgg gggctcttcc atccgatggt
    20941 ttaccatcgg acaacgggta tccaatccct tcgatgcaaa ctgctccaaa ctgtttgcat
    21001 ataacattct ctctctcacg cgacgataga gttgggtaca ttgcctccaa ttctgccgaa
    21061 tgaacaaaat gaatgctctt gggtagttcg ggagtaatcc catcgacgtg agtggcaacc
    21121 accatttcca tttcgcagat ggcttcataa atagcattaa ctgttttgcg aaagtattcc
    21181 acgcatcttt ccgatgcatc gatacgtttt tcccagtccc actggtcaac gtaaactgag
    21241 tgaattgcat cgggggtttc atcgggcctt agtgcattca tatcggtata tattccgcta
    21301 ccacagggta attccaaacg agcaagtgtt aaccgcttcc attttgcaag cgattgaaca
    21361 atttcaattc gtttgccaat ggaactggcg gtaaaactta ccggcttttc aactccgttt
    21421 aaatcgtcgt taatgccaag accagcctca actataagcg gtgcggttac ccgtcgcagg
    21481 tttagctttt ttgccagcag ctttggaaag gtctccttga ttagggtgat agcgtcctcg
    21541 gtttcaaccg aacggcaaat gtcgcctgta agtgtttcaa aaacttttga ttccatggtt
    21601 atattatttt ggttagtgtt gatagtttga agtttgaaaa aagaatgccc ttcgcggaat
    21661 taccgggaag ggcagggtaa agcataacca ttcccggggg gtggcggacg attattgttg
    21721 ttattattta ctctttgcag catatggaat gtgttaaaca aaaaaggcct tcctgttttt
    21781 ttaggaaggc cttgaactag tattgtatct tgctcaccaa acgccaaaac cttcccgttt
    21841 caacaggtta ttgttgaaat tggaattatt attggctttg gagtatcgaa tcattttaat
    21901 ttcacattta cacgtgcaat atcgaaaaag aatataacaa atgcaaattt ttattatgga
    21961 ttttgtttag ttctttcttg tgtcaagaca tgaaagaacg tgtaaatggc tctttgcact
    22021 gcttatctgt tgcgatgcaa cgtttcaatt tacctctttg gcatatgcat tgcgtgcatc
    22081 gcaatttatg agagtagtta ttgggttaca aatattaagc ccctacgggg ctggaagcat
    22141 tgttcgttgt ttgtgaaacg ttgttcgttg ttcgtgaaat tgacctcacc caagcctttt
    22201 tccctgataa ggaatggaga ggaagaagca agaagaggca taaaagcctg acccgaaaat
    22261 gggtcggggg tggaacgtgg cggtgggcat tgtaagcgat accaaatgag ccacgccgca
    22321 ggcgtggcga atagtatcgc gtggaagttc cacccacggg gtacccatcg ggtcgggctt
    22381 ttcagccttg attttctttg gttctttctt gtatcaagac aagaaagaac atgcaaaccg
    22441 ctctttgcaa tgctttactt ttgcgatgca acgcatcacc attactcttt ggaaaaagta
    22501 ttgcgtgcat cgcctataat tttgaatacc acaataccca gggttaaaac cctgggctaa
    22561 tgtgatttgc ccttttaggg cgaataatgt gcgtgtcgaa agacgtgtgt taactagtga
    22621 gttcttaaat cttaaataaa tcgagattcc tcgactagct cggaatgaca aatagcgttg
    22681 ttcgtgaaac gttgctcgtt gttcgtgaat gccattctgt taaacaccgt gttgcactgt
    22741 gtaactctgt gatacttgct gttgcctgtt tcatgatacg tttgtcatgc tgagctcgcc
    22801 gaagcatctc tttgcaccta caactcttca ttaatgagat tcctcgacat agtctatccc
    22861 gatataatcg ggactcggaa tgacaaatag cgttgttcgt gaatcgtttt tcgtaaaaag
    22921 aaccttgtgt attaacctcc tctaaactta atcctttatc cttcgtgatt acactaggca
    22981 gtacataatg ttctatcccg tttaacggtt cacggttcac ggttcacggt tcacgcttta
    23041 ccctttatcc tttatccttt atcctttaac cttgctatcc cttcaaccaa ctcctcgatt
    23101 tccaaatcgg aaaaaccctt ttgcgaggca atttcgtaca gggttcccca aacaggctca
    23161 ccacaaatga tgcatacaat acctttctca cgaagaaacg atacagcctc tggaacctgt
    23221 tcaaccagct cttcaatgct aatatctttc gtgatttcca ttttggctat tcgttgtgta
    23281 taatcacctc gtgcgttagt ttcatgcctt ttttataggc tgcgctaaca ccgcagtact
    23341 tctcgtcgct aagtttaaca gccttgttaa cattctcagg gttgaggtta ttgccccaga
    23401 actcatatat aattttcatt tcaacgtaat gttttgggtg ctcatcggtt tggagggctt
    23461 caacgctaac gcggaatttt tttagatcct cgcgcatctt tttaagtatc gaaaccacat
    23521 ccattcctgt gcacccggca agggcaacca gcatgagggg tttaggccga ggtccacggt
    23581 tggtacctcc aaccttttcg tcggcatcta taatgagttt gtggccgtta acttcagttt
    23641 caaaggccat gccgtctagc caatcggcct gaatgcttat tttatccata aatgtagatt
    23701 ttttaatata acgttaccaa aattgatttg ttcaaagata gctagtttta tggctttgtg
    23761 taatgtgttc aatggagcgt tttgtttttg gcgtttggaa aaaaatctat ggtaaaaaaa
    23821 aatagagccg gagttatcta cggctctatt tggggtttaa gagacttttt actttcttcc
    23881 tatgataacc tttttgatta aggtatcgcc ctggctattg gaaattcgga taaagtacat
    23941 tcctgcttgc agcctattta cattaaacct ttgggtaaag ctactggatt gaataatttc
    24001 aacctgttca accgttaagc ctgaaggtgc gatgaattca atcttgtaat tgctggttct
    24061 atcgccatca acctgaatgt aaagctcatc gtttgcagga ttgggatata tggagactct
    24121 ataaggggta tcgataattg tttcagcgct aaccggccac caaacaacaa ctgtatcgga
    24181 agaaacacat ccgtgcgagt cggttaccct taaccagtac ttgccccact gtccgtttga
    24241 gatggttatg gtggggctgg ttgcaccggt gttccaaagg taggttacac cacctatacc
    24301 tgactctaac tcgtatggaa gtgaatgttt aattgtgtcg gagccaaagt caaagtttgg
    24361 caactcccaa acattaacag tgaactcggt tgaatcgcca ttgtcgcctg ctatggttgt
    24421 gaagcaggtg aagttaaatt caccagtgct aagggctatt ggtgtgctaa atttaaactg
    24481 cacggtgcca ttttgcggta ggttcgatgt gaatgtatac tgctctttaa cgggtgttga
    24541 ggtgccaatg tagtagttga catcaatcgt ctcacccgtt gtgaaggttt ttgtcccgcg
    24601 gtttaggagt tctaccttaa cggaatcggt tttgttgtcg tagcacttat cagctggtga
    24661 aagcaggctc gatacaatta tgtcaagggt ttccaggtaa gttatataaa cctcatcgta
    24721 gccatggcag ccattattgt cggtaacggt aaccgagaaa agccctgttt cggtaacggt
    24781 aaatgtttgg ttggtggagt tatcttgcca caggtaagta acaaatccgc ttcctgcatc
    24841 aagggttaaa ggctcggttg ttgagcggtt agaccctaaa tcaactacgg ggagcggatg
    24901 aactgtaatg tcaacattag tgctattgct gcaaccattt gcatcggtat aggtgtaggt
    24961 taaggtatgg gtgccggcgc cagcgttcga tgcattaaag gtagtagtat tggttccggg
    25021 gccactgtaa atacctcctg atgggttgcc ccctgaaagg gcaaattcgg gttcgtcgaa
    25081 gcatactgca tccagaggat cgtgggtaac agttggcaaa gggtttatgc ctatattaat
    25141 cgtgtcgcgg ttaatacatc cgtgtgagtc ggtaacctct gaccagtata atccggcagt
    25201 tgaaacatct attgtctggg tggtggcact ggtgctccac aggtacgatg aaaaccctgc
    25261 acctgcatca agtgtgtggg ttgtaccctg gcagatggca gtgtcaatcc caaggtttac
    25321 attgggaagc ccccatacat cgatggtctt tgaggaagaa ttgttaaatg ggtttacatc
    25381 atcatttgag gtggtgtagg cttttattgt atatgtgctt ggtgtgctaa aattgaaagc
    25441 tttggtgaag gaatgggtta acttgtcgcc tggcttaaac tttgaggtca tggttattgc
    25501 ttcggtttgc tgtaggatgt tatccacctc gtacgagaca aataggtttt gtcctatctg
    25561 aagtgtatca gttccagcat tttcaacttc aacctgaact tgctcggttg cgcttaaggt
    25621 acacgatgta ctgggtgcga taatagttgt tactctataa tcggggcgga ggatggtaat
    25681 gttaacggta tccctatcgg agcatccgtt tggtgcggtt gtttccaccc agtaggttcc
    25741 cgtattctca gcaaagtagt acctatcggt tgagttatcc tgccacaggt aggtgtagcc
    25801 tgtcccttca ccgccgtcga gggttacgct tccggtgcgg tcaaccctat cggggcccaa
    25861 attaactacg ggcgattcat ttattgtaaa cgaggtcaac agtgtatcgt tacctgtgtt
    25921 agtgtcgcca gtatagattc cataagcaat catgtcgcgg gtaagaggtt ctgataggtc
    25981 aatcttttca gtgaagttat agagcaaaaa ttgacccggg tgtaagtccg ataaaagggt
    26041 gtatgtttcc tcggcaaagt agctttcgtt tatctggtaa ccaaacctta cctgctgccc
    26101 tgcctttaat gtgtcattac ccatgttggt gtacttaatg ctaacggttt gcttttcagg
    26161 ggtgctgcac gatgtggttg ggttgaccac ctgggtaatt gagtaatcgt gctttaggaa
    26221 ggttacgtaa accgagtcgg tggttgagca aaaaccattc tcagtaacag taaccttata
    26281 tttacctgtt tgggaaaccg taatggtttg ggttgtttca gcagtgttcc acaggtagct
    26341 atcgcgtcct gctccggcat ctaaaaggta actgttgtta cgggtaaata ctgaatcgcc
    26401 caattcaaga cttgggtagc cataaatgta aacggtttgt cttaaagtat cgttctcggg
    26461 tttgttatcg cttgcagttt tggcaacaaa ctcaaagtta taggttccaa ctgccgtcat
    26521 atcaaccccg ttgccaaaga taatttcttt ggtttcgttg ggataccatt cggaatcaag
    26581 agtaagggta ggagaatcct gtagaacgtc gttaagttta tagctaaggt tgatgtcggt
    26641 gccagttgta atggtgtcgg ttcctgcatt tctaataatt attttgggga atacagtacc
    26701 tgatatttca caagcggttt gaggaactaa aacagtatca acggtaatgt cagagaatcg
    26761 taggtcaaca aaaagagagt cggtggcagg gcaactgtgt tcatcgttaa cagtaactat
    26821 agcccaacca gtttgggttt gattaaagaa ttggtttgta gaaccatcgt tatgccagta
    26881 gtaactattc catattcccg catttaaagt gtattcaatg ttagtaacct taacggtgtc
    26941 ttttatgagt cccatggtgt cccttaggct aaaggttgga tatccccaaa cgtctatggt
    27001 tgagtttatg ttatcattat ttgcatcgtt atcgagccaa acgttagccc atgctttaag
    27061 tgtatatgca cctacatgca tgtccaggct atcggtaaag gcgaaaataa tcgaatcgcc
    27121 tgggagtatg attctatccg atacccatac cgtatcaact ttcgggtcgc tgttaaactt
    27181 gtagctaatc ctgatggtgt cgccaatggt tagagtatcg gtgccaaaat tcttaacatg
    27241 aaccttaatt ggggttttgt ccgacggttc acacgagcta accgggctgt cgaatgcagg
    27301 gatgccaaca tcgcggatta ggcgatgaac ggttatggaa tcccatgcct gacagggtat
    27361 ggagttctcg agtagtacat agtattttcc tccatttgga tcactaaccg tatcggttgg
    27421 tgttgtgctg attacatttg tcttatcggg ggctttatac cacttgtagg ttaccaaggg
    27481 gtctgggttg gtggcatcga gtacaaccgt atcgggttgt aaagtccaaa tatctgggcc
    27541 taattccacg tagggttttt ggacggtaac cagtttggtg gctgcatcgt ttgagggtga
    27601 ggaatagaaa agcgggtcgg tctcaatact ggttgaagcg gtaaggttat agtcgccgct
    27661 aacattcatg ttaaaggcac ggctaaagtt gtagtcaagg tgatctcccg tattcaatgc
    27721 actggttaac actatggtgt cagtttcgga atcgggaacc atgctgtggt taatgttaat
    27781 tccaacaaca atggtgtcgc cagcaatcat gttacggatg ccgtaattcc ttatcctgat
    27841 ctgtatgggc tggttttggt tcaaatcgca agcggttgaa ggggtcagga tgctttcaac
    27901 tcctatgtca aacggggcct cgtatagctt aatattatca atggccgttc cgccatagtt
    27961 gttgttggct ttatcggcct taaagcctac acggaaaatt acattgcttt taccggctaa
    28021 tgttgcaggt agttcgtggc gcgaacgttc ccaggcagct cccaatccat tccagccatc
    28081 aacgccaaga gcgctaacca aactatcggt gtaccagttg aagtattcat catgggtggc
    28141 attgttgccc acatgttgcc aggtttgtcc attatcgatg gagtagtaaa gggttgcacc
    28201 atccacctca ggttcagttc tgttaaatac attaaggtcg ataatgggat tggttacacc
    28261 gctgaaatcg aaacaggggc tttgcagcca tgccgtttcg gtattaaatc tggaacctgt
    28321 aatccaaaca tcatcaaaat tgatgccttc ggctacattg gcatcgccat tcgatttcag
    28381 aacaaaccga atgtaaagtg ttcctgaatt ggcaagacca ttggggattg gtatggtatc
    28441 aacatcccag ttttcatgag taactgctgt gccgccagtt gtaaataggg tttcccatgt
    28501 agttccattg ctgctggctt gaaccattgc ggtatcgccc gctgcaagtt tatgccaacg
    28561 ccaagcgtaa agtgttaagt tattgtagtt tgacatatca attgctggcg atttggcgta
    28621 ccaggttgca ttgggttcat acatgccctt gttgacccct tgcctgctta aatcggtacc
    28681 aatgcagtat tgcccgctgt aagtgtatgt tggaatggta tccccttgca tgataatatg
    28741 attacgctca aactctccgg taaatgtcca tcccttggcg gtttcaaaat cgtcgtagaa
    28801 aattgtatcg gttacccctg taagcaatga gccataattt tgggataagc tggtagccca
    28861 tgcctttgag cctccgctca tggagggttt tgaaataatt ccccattccc aaattggatt
    28921 tccgaatttt cgccagtaac catcgttgct ctcaaaggtt tccgtatagg gtagggtgta
    28981 tgttggaaca atatagattt gtcttgaaat gccatcgttt gatgcatcct catcgccggt
    29041 aaggcgtgtg cgtgctctaa cctgtttgat tccgggtgtg gagaggttaa ccttagtact
    29101 aaaggtgaac tgcactgtat cgcctggtgc aattacttgg ttaaggtagt tggtcaccca
    29161 tgtaactcca ttattgaacg agtactgaac gggtattgaa gccggtgagg gttcggcacc
    29221 catgttggca accttaaccg taacactatc atttgccgaa tggccacagc ccgacaaagg
    29281 ataaacccat tccacaacac caacatcctt ggttatgtag tcgcctgtta ccgtaaaatc
    29341 atccacattc caacccgagt aggtgctgta gttatcggtg gggccaagct tatacataaa
    29401 ttttaatttg ttggaacgcg atacctctga aggaacgtta tgcgatgttt ttagccaaag
    29461 attgtcggta aagtagttgt tgttgtacca tatgttgctc caggtacttc cgttatcctt
    29521 gctaacatca actgtggccc tatcccaaac ctcaatgtta aggtannnnn nnnnnnnnnn
    29581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29641 nnnnnnnnnn nnnnnnnnnn nnnnnagatt gtggttgata ttgttaaacg tagccaaacc
    29701 gcttacgaaa gaacgttccg ttgaaagcgg tgttagcgta tcgagtatgg cagttgtggt
    29761 gaagtaaagc tttacgccat tggttaaaat atcgttatcg tttgtgtttt tcgactttac
    29821 aactattgtt ttcagtgcag cggttcctgt gttcccgaat attttaaaac ctaacctaag
    29881 aattggttcc tttttggaac cactgggtat aaaggttaaa tcgggatgaa ccacggtgag
    29941 ctcactaaca tatcggggtt gggttccccg ttcaattacc tgcacatcgt cgatacaagt
    30001 accaaacccc cagttggttt gcccctcaaa ggcaatatag taggtgctgg tggggttcgg
    30061 taggttaatt ttgaactctg tccaatccgg gatggggtct aaaaattctt gtagtagcgt
    30121 ccaattatcg gaaatgtttt tcttatagta aacccttagg taatcccagt tagtatttcc
    30181 ggagaatgtc cagggaacct gacacagcca aaaactgagt tcaggctgta tagccccggc
    30241 aaggtcaata gccttggtaa ttagctttgt ccgttcttgg tttgtgctct gcttaaagaa
    30301 aatagcattg taggttccgg aatgggcgct aggcgggttg cgggttatgt cggtttgcca
    30361 ggcaggtaca gtccagttgt tgtcgttggg gctatgcccc ccgttacggt agcgccaggg
    30421 ctcggttccc gaaacgtact cctcggtcca gcctgttggc ttagttccgt tttcaaaacc
    30481 ttcgctaagg taggtttgag cttttgcata gcttattgta agaatgcaaa gcgtagtatt
    30541 tagaattagt tcttttactt tcatataccc gccatttatg gttttgtttt gtaagatagt
    30601 caaaacttag caaaccgttt actaattttt acgttatatt tataaatagg ttgataactg
    30661 cattaacaat tttttagggt attggtttta tggttaacaa cggctgattt tgattaagct
    30721 ataacattta ctgacaaacg gacgtaaaat gtaaacaaaa ataatatatt tgtcgataaa
    30781 tgatataatg tctgaggatg cttaagcaca ccataaaata ctggattcta tttctctact
    30841 tacaatttgt agctgtgtgc gctcacgccc aaacagatac tgaattctgg tttgtggtgc
    30901 ccgaggttac cctaagtcat tacgctcccg gtggggcaaa tgcttacttt aagttttcaa
    30961 ctggacttct accggctact attaccatta gcatgccggc caaccagtat gatcctgtta
    31021 caaaccccac aggtttccca gatatagtga ttaatatggc tgccaacagc tttcatgaag
    31081 aggatctatc gtgttggata gttcgacggg taaacccttc agtgcccgat tgtaatagta
    31141 tacccatcga tacatcgtat gctttagctg attatgccga tgttaacagg ctcgagaata
    31201 aacccttaaa tgcttcaggt atcaataaat ttggaattcg aattacctca actaacccaa
    31261 ttactgcata ctgggaaata tcaagacgaa ataataagga tatctgggct cttaaaggac
    31321 gaaatgctct tgggttagaa ttctttgctc cttttcaaac acatggaaac aatacaagca
    31381 tattggacac cccaagtgcc attgatgttg ttgccacaga ggataatacg attgttactt
    31441 ttcagttacc cccaggtatt gaggcttctt atggaaatcc aatgactaat gttccggcag
    31501 gaggtacgct tacccgtacc ctgaaccgcg gtgagacttt ttcgctattc ccaagagcaa
    31561 aaaacaaaac cattgcattc aaattgaggg gtacaagggt taccgcaaat aagcccattg
    31621 cagtaacatt aaaggacgat tctttttatc acacatcggg tggttgctac gatattgcag
    31681 gcgaccaaat ggttccaact tctattgcgg gtaaggagta tgctatcatc agaactttcc
    31741 tcaacaacca cgatcatatc tatattttgg gtacccaaaa cgcaacaagt gttaccgttt
    31801 acaacactgc aggcggtgta gttgcctcaa ctgctattaa tgcccaacag caactttact
    31861 accagattcc tactactgaa acctactacc gtattgtagc cgataaacct gtttatgttt
    31921 ggcatgttgg tggctttgga tgcgagcagg gtggtgccat attgcctcct atcgatatat
    31981 gtaccggtag tacccaggtg gcatttgccc gtacgtcggc cgaaaacttc tatataattc
    32041 ttatggttcg gcagggagcc gaaaccggtt tcctttttga tggttttgtt cgtaacgacc
    32101 ttattaatcc ggcatccttt attcctatcc ccggatcaca atggagtgtt gcccgatttg
    32161 gatcttttgc cccagattca cttcacattt tggttggttc ccactttatt gaaaatacag
    32221 aagacctttt tcatatcggg attgttaatg gcggttccag ctctggctgt ttctacggtt
    32281 acttttccga ttacaacgaa ctcgatgttc aggctgtggt agcaggaaca aactccgatg
    32341 tgctaaaaac ctgctacggc aatcctgttc agctttatgc ctacggcgga accgattacg
    32401 tgtggacccc cgatagtact ctgagcgatc caaattcaca gctcccctgg gctacaccaa
    32461 aggtaaacac aaagtataag gttgtggttt caggtgcatg cgatatgacc gatagtgcct
    32521 ttatcgacgt gctggtttcc actccattaa ctgcaagttt tattaccgac cgtgttgaag
    32581 ggtgtgcccc tcttacggtt aactttgagg ataaatctac aggtattacc tattggcgtt
    32641 acgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngttcag ggttgtaatc
    32761 cccaaaccgt tacctttaca aatagttcat cgagcaatac tgccgatacc tatctgtggg
    32821 agtttggcga tggaatgaac caggttacca ataacccaac cggcacggtt gttcatactt
    32881 tcacaaacac cgttccccgc gatactacgt atagcatgcg aatgattgct acatcacctt
    32941 tcatgtgccg cgatacggct actgccagca ttaccattta ctcattcttt actgccaatt
    33001 ttaccaccaa tatcagttca ggttgttccc cagtaaccgt gaatatcgat aacctatcgg
    33061 ttggcgatac agcccggtac gtttggacgc ttaacggtgt acctttgaca aagacaaccg
    33121 gctgcgatac caccctggtt cttaccaata ctaccaatac tcatattgat tatgtaattc
    33181 agctgcaggt ttttaatacc ggaaatcgat gcagcctgcc agtttcccgt accgtaaggg
    33241 tttaccccga ggttaatgct gcctttacca ccccacaagc gcagtactgt aatttaagta
    33301 gtgtaaattt taataatagt acttctccgg cttcaactaa ccccggcaac gtggttaata
    33361 cctacagctg ggattttggc gatggggctg cttcggcact tgttaatcca acccacgttt
    33421 acgataacca gacaaacagc cctaaggatt acgttgttac gcttacagcc cgcaaccaat
    33481 ttggatgcca ggatgtggca actacaacca tcagaatatt tagtcagatt tacgccgatt
    33541 ttgctgttac acctgctgcc cagtgttcgc ctgtaacggc tagtatcgat aataactcgc
    33601 gtggaggaat agtaacctac agctgggact atggcgatgg aactcccaca agtggaacaa
    33661 accttgacca tacacattct tacataaaca acgaattgac tcttattcac cgaactataa
    33721 ctctaacggt taccaacgaa gggaattgta ccgacaccga ttcgctcgat gtggtaatct
    33781 acccttcggt taccgcaggg tttactccaa gtgttacagc tggctgtaat gaacttaccg
    33841 taaacttcac cgataactcc aaggctaatg ctactattta taattgggat tttggcgatg
    33901 gtagttcatc aactcaaaaa tctccctccc atacctttgt taatctaaat ggggttgatg
    33961 aaacctatcc ggtaacgcta accgtttata ccgattatgg ctgtacagct caggcttcaa
    34021 ccaatatcac tgtttaccca tttatcgatg ctcaatttgc tatcgatacc agctttggtt
    34081 gttcgccgct tgatgttagg tatgtttata ctaaacaccc tggtattact gaataccgtt
    34141 gggattggga cgataatggc accgttgacc aaaccacact ccctgcagcg cccaatacaa
    34201 ttacccatca gtttgtaaac caaacaggtg ttgttcaaaa ccccacaaca cgcttaaccg
    34261 tagtaaatag ccacggttgc agcaagacaa cctcaagcac aatctcggtt taccctgagg
    34321 tatcggcaat atttgtccca acggttgata ggggatgtaa cccattaaat gttacgctga
    34381 ataataatag catttttaca ggcgtgggaa cacctctggt taatacaaat tactactgga
    34441 cttttggcga tggcggttcg tcaactacca agagccccaa ccacatctat ataaataatg
    34501 atcccaataa caatgccatt tacaataccc gtttaaggat tgtaagtgag tacggatgtg
    34561 ccgattctgc tttccatatt attaaggttt acaatagggt ggaatcgcat ttcactttag
    34621 agtatgctga aaattgtacc ccctttgatg taacatttaa tcctgcagcc attggggcat
    34681 cgacttatat ttggacttac ggtggcgctc caggcttacc agcctcagaa acctttgcca
    34741 atggaaaccc attcacccgt acctttacca acattgatcc taacaatatt gcaacctata
    34801 ccgtaaccct tgaggctagg aataacgagg gctgtcctgc ctttgattca cacagtattg
    34861 aggtttatcc tattgtggat gctcaattca atgcatcaac cgttgtcgga tgctctgatc
    34921 tggatgtaac cctaacatcc acatctaccg gtggtagctt ggtttacctg tgggatttgg
    34981 gtaacgggca aagcgctagt acgccaaatg taagccacac ctttactaac cgtggtagta
    35041 ttgatagcgt ttacgccgtt aagcttaaaa ccattaaccc acttggatgt agggatagta
    35101 tcaccagaga tattacggtc caccccaagg ttgaggcaca gtttgtgttt gcccagcaat
    35161 cgccatgtac tcctttttat atcgatttaa caaatacctc gctcaacgga aatacattcc
    35221 actggtacac ggtttacaat ggcgatacca tcacaaataa taaaaatact ttccagtacc
    35281 tgttcgataa ctccacgctt aacgatatac ttaccgannn nnnnnnnnnn nnnnnnnnnn
    35341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35401 nnnnnnnnnn nnnnnnntaa tcactgttta ccctctggta aaagctgact tccagtggaa
    35461 tttatttgag ggttgtacac ccttgactat taaccttaac aattcgtcaa catcaccatt
    35521 gtataagtac cgctgggatt ttgacaatgg aatgccttac ttctacagcg atcagcctgg
    35581 atcagtaacc tataccaacc ccacaaattc gccaccagta attctttcac ctacaataac
    35641 tcttcgtaca agttactcgg gcgacacgtt gtgtatcgat agccttaagt tgcccattag
    35701 ggtattcccc catatttatc cagaattttc tggaaatcta gcgggttgta atccgcatac
    35761 ggtgaacttc acaaatcaaa cagttgctta cagcacaaac aatagctact actgggattt
    35821 gggtagtggt gtaaactcac ttttggctaa ccccacttta acctattaca ataccgataa
    35881 gtttgtagat agcacattta ctgtgaagat ggttgcggtg agcgagcatg gctgtaccga
    35941 tagtgttttc catcaagtgg tagcgcatcc cagaccttac gcagccatgg agctaacagg
    36001 tgagtatata tcgtgccctc cattcgatgt tgaaattgaa aacaactcgt tgggtactaa
    36061 cctaaccttt gagtatgatt ttggggatgg cgccgattcc accactacca gccaggctaa
    36121 tatgcggcat attttccata acttaagcaa tcaaactcaa ccctaccaaa taaaactgcg
    36181 cacagttacc gagtatggtt gtgacgatag cgttttccaa accatttatg tttttcctga
    36241 ggtagttgcg tcgtatgtgg ccaatcccgg ctatgccgct tgtagcccat ttgaggttaa
    36301 ctttactaac aataccatta acgcccgatt ctatgagtgg gattttgccg atggcatcac
    36361 ctcgtcgttc ccaaccccaa ggcattcttt cctcaacttt ggggagaccg actcagtttt
    36421 caacgtagta ttatatgcat cctctgagtt tggctgtttc gatcgcgata cacagcaaat
    36481 aatcgtttat gcaacaccaa ttgccaatat tgccgctgag ccacccttgc aggtattccc
    36541 taatgctacc tttaatattt ataaccagag tagccctgct gccagcagct ggaattacaa
    36601 ttgggccttt ggcgataata ccttcagtac cagtaaagat cctctcacta agacatatac
    36661 taggtggggg ccaaaggagc aagactttag gtaccgcatc accatgcgtg tagatgcgcc
    36721 acatggatgc tgggatgccg ataccgtttt ggtttacttg ctaccaacag agccagaagc
    36781 tttctttaca agtagtgttg actcggcttg cgctcccatg gaggtgcaac ttgttgatgc
    36841 ctcccagttt gccgatagct acctgtggga gtttggcgat ggagctacat caactgaacg
    36901 taatcctcgc catacataca ctgagccagg ttactacacc gttaagttaa ctgttactaa
    36961 cgacgcaggg tcaaggttct actataagat ttttcgggtt tacgagagcc ctgaagccag
    37021 ttttgccatt tttccccaac gcgtaatgtt gcccgatgcc actattcacg cgtacaatct
    37081 atcaactcag tttactcgct gccagtggga ttttggcgat ggttaccaaa ccac
//
LOCUS       DAOOWJ010000012        36707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1175_length_36707_cov_7.614537, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000012 DAOOWJ010000000
VERSION     DAOOWJ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 36707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36707
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1175_length_36707_cov_7.614537"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..2139)
                     /locus_tag="PLW22_02320"
     CDS             complement(322..2139)
                     /locus_tag="PLW22_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOU73569.1"
                     /translation="MKKFIRILKYLYPFKWYVVINVFTNVLAAVFSLFSLAMLIPFLQ
                     LLFGTVPLVEVKPEFVISAKGLAQYLNYYLSEIIKTHDRASALLFVIVLVLVASFLKN
                     LFTYLSLYYLAPIRTGVLQNMRNLLYRKVVDLPLGYFTEEKKGDIISKMTNDVNEIEV
                     SIIRSLEMFFRDPIIIIIHLIGLIYISPQLTLFVLLILPLTGGVIGRVGKNLRKTSFK
                     GQTKMGIILTMIEETIGGLRVIKAFNAENKVISRFESMNSFYSLLMKKMWRRRDLASP
                     LSEFLGTVVVVLVLWYGGNLIFAGKGNLGPEALIAYIGIFYMIINPAKSFSNAYYNVL
                     KGLASADRVDTILNAENPIKVSAGAKIIESFNHDIEFRNVSFKYQDDWVLKNINLTIK
                     KGMTVALVGQSGSGKSTMVDLIPRFWDVTEGEILIDGVNIKDYDIASLRNLMGNVNQD
                     PILFNDTFYNNIAFGVDGAKMEDVVNAAKIANAHDFIIATPNGYHTNVGDRGSKLSGG
                     QRQRVSIARAILKNPPIMILDEATSALDTESERLVQDAIDNLMKHRTSIVIAHRLSTI
                     RKADLICVLHEGEIVERGKHDELLKLNGYYARLHSMQLL"
     gene            complement(2223..3569)
                     /gene="purB"
                     /locus_tag="PLW22_02325"
     CDS             complement(2223..3569)
                     /gene="purB"
                     /locus_tag="PLW22_02325"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOU73570.1"
                     /translation="MLNHSLHSISPIDGRYAKKTESLSGYFSEFALIKYRVWVELEYF
                     IALCEIPLPQLQGFDKSKFTILREIYKSFGESDALRVKEIEKVTNHDVKAVEYFLRER
                     FDAYGLESYKEFIHFGLTSQDVNNTAFPAMLRDSLKTEYLPLLEKLLAKLKELSTQWA
                     DIPMLAHTHGQPASPTRLGKEFRVFVERIEVQLEQLKHIPYSAKFGGATGNFNAHIAA
                     YPSIDWVGFANQFVNYKLGLHRSQTTTQIEHYDNLAAMFDCLRRINSILIDFSRDVWM
                     YISMEYFKQKIKEGEVGSSAMPHKVNPIDFENAEGNLGLANALFDHMANKLPISRLQR
                     DLTDSTVIRNIGVPIAHSFIALKSILNGLDKLIVNTDAINRDLQSNWMVIAEGIQTIL
                     RREGYPNPYDALKLLTRTNQQVTSDTFTNFVNSLNVKDSVKRELLELTPFTYLGIK"
     gene            3693..4448
                     /locus_tag="PLW22_02330"
     CDS             3693..4448
                     /locus_tag="PLW22_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOU73571.1"
                     /translation="MKKYKVSAISYLNTAPFAYGLKHSPVFNKIELLYDYPAECARKL
                     ANNQVDLSLLPVGALSDFSSFDIVSNYCIGSIGKVRSVALMSNSNLSDIKRIYLDYQS
                     RTSVILVKILCQKVWNITPDFIPLKPSDNYKLLKEYEAIVLIGDRVFEAENHFAYVVD
                     LSNEWFNAYGLPFVFAVWATTKRLPKELIEELNTGLELGVNSIEKAVDEFSPLSISRE
                     DAITYLKQNISYTLDEVKFKAIKLFQTLSAKLN"
     gene            4488..5894
                     /locus_tag="PLW22_02335"
     CDS             4488..5894
                     /locus_tag="PLW22_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOU73572.1"
                     /translation="MKTLRILRLFILLLLAGIHIPAQAQDSLPASKLIYKINIKENIM
                     PAAWRSVKVGFEEAQNLKADIILIHMNTYGGMVDVADSIRTKILNSKIPVWVFIDNQA
                     ASAGALISIACDSIYMRKGGSIGAATVVDQSGNVVPDKFQSFMRSTMRATAEAKGYDT
                     LISGNDTILKWRRDPRIAEAMVDPSIYIEGIIDSGKVLTFTAEEAIKHGYCEGKAENV
                     NEVMEQAGIKSYSIAEFRPSLLDRMIGFLTNPFVSGILIMIIIGGIYFELQTPGVGFP
                     LAAAAIAAILYFAPLYLEGLVEHWEIIIFVVGVILLMVEIFAIPGFGVTGISGIVLMV
                     AGLTLAMIDNEIFRDPVNFNLMTILKPFGIVVLAVFIGLIGGIALSRKLLTSPLFPNL
                     ALQSNLNKEEGFVGIDTKIKNMVGAEGVAITTLRPSGKIQIGNSWFDAVAEFGYIEKG
                     SKVKVSKDESGQLYVVKA"
     gene            5899..6336
                     /locus_tag="PLW22_02340"
     CDS             5899..6336
                     /locus_tag="PLW22_02340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOU73573.1"
                     /translation="MESITIRDFQPSDYEGLSELWLLTNLGNPARGDNLEIIQQSIDL
                     GGKLLVAVNSDGMVIGTSWMTFDGRRIHLHHFGVHPNYQRQGIGKMLTRESLRFVKAK
                     GYQVKLEVHRNNIAAINLYKQFGFQYLGDYDVYIIRDLNSISI"
     gene            complement(6436..7065)
                     /locus_tag="PLW22_02345"
     CDS             complement(6436..7065)
                     /locus_tag="PLW22_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOU73574.1"
                     /translation="MKTLPFLVAVLLSLVSNGQNDSITKLPEPSRVKGKNIMEAFANR
                     ASVREFDTQELDLQEIADLLWAANGINRPAEGKRTAPTAMNTQDIDVYLVNSKGAFLY
                     DAKAHELVLISSGDYRSAVAGSQQNFAKAPIFLVIVSDVARFQRGDESQKMTWAAMDA
                     AIVSQNINLFCAGVGLKTRPRVWMETEKLKEILKLRETQRPMMNNPVSR"
     gene            complement(7078..8112)
                     /gene="mltG"
                     /locus_tag="PLW22_02350"
     CDS             complement(7078..8112)
                     /gene="mltG"
                     /locus_tag="PLW22_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOU73575.1"
                     /translation="MKKFVKFGLVIALVGLAVGVLLLLRLYFTFRATNVAVSKETLIY
                     IPTGSNYNDVLNILDKNKVLKSIDRFKRVAQYYDYEKSVKPGCYAFKPGMSNSSMVKM
                     LKYGHQKPVKVTFTGMRTWQQLAQRISSQIEADSVSLINLFTSDSVAFNRGFTPSTFM
                     AMFIPNTYEFYWNTHALGFINRMYKEYNRFWNESRIHKADGIGLSPVEVAILASIVEE
                     ETNVKSEMPIIAGVYLNRLRKGIPLQADPTVKFAIGDFTIRRVLTRHLAVDSPFNTYK
                     HKGLPPGPIRIPSVIAIDAVLNAQKHSYLYFCASPDFSGRHIFAQTLAEHNRNAAEYR
                     RALNMERIYR"
     gene            complement(8202..9395)
                     /locus_tag="PLW22_02355"
     CDS             complement(8202..9395)
                     /locus_tag="PLW22_02355"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU73576.1"
                     /translation="MEKVSARIAALEESQTIAMSKKSRELAAQGIDVISLSVGEPDFF
                     TPDFVKDAAKKAIDQNFSFYTPVAGYKDLLEAICGKLKRENNLEYQPDQIVVSGGAKH
                     SLSNVLMCIVDKDDEVIVPAPYWVSYVELVKLAEGKSVVIETSLESDFKITPKQLEAA
                     ITPKTRALLLCSPSNPTGSVYTYDELKAIAQIIEKHPNIIVISDEIYEHINFIGKHES
                     IAQFDSIRDRVVIINGVSKGYAMTGWRIGFSASAKWIAKACEKLQGQQTSGVCSIAQR
                     AAIAAFNSDGEYTRQMREAFRRRRDLMYEGFSSIPGFKLNKPQGAFYIFPEISELLGK
                     QYNGETIKTSDDLAMFLLNHAHVAVVPGSAFGSPKCIRLSYATSDDKLKESIERIRKA
                     VALLK"
     gene            9502..10017
                     /locus_tag="PLW22_02360"
     CDS             9502..10017
                     /locus_tag="PLW22_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73577.1"
                     /translation="MFAEILKITIPALMVFLAGYLALRTLIKNETQRQKAELMFNTIK
                     VSLPLRLQAYERLILLMERISPESIIVRVNKKGMLASDLQSALLGNIRAEWEHNLSQQ
                     LYVSNEAWEMVKNAKENIVKLINICSEKVAPNDSSFDLSKKILETYAELEINPTSLAI
                     EFLKKEVKEIL"
     gene            complement(10026..10850)
                     /locus_tag="PLW22_02365"
     CDS             complement(10026..10850)
                     /locus_tag="PLW22_02365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU73578.1"
                     /translation="MIKLVVTDLDGTLLRSDHKFNGEDMVSLCRLGEMGIIRAIATGR
                     SPFSASTVLPDDFPIDYLLFSSGAGIMRWSDKSIIHANQLSPEVVQYVIALLVEMHAD
                     FMVHEPIPLNHCFHYHSSGKLNSDFERRISLYKDYSQPLITGVPFPGPASQVLAVLPN
                     DIERFNAISKKLNGVQVIRATSPLDGKSIWLEIFPEGVSKSGGIQWLCSYIGGIQPRN
                     LLALGNDYNDIDMLNLAGEAFVVSNAPSELKDKFQVVPVNDEAGFSHAVKRVLKID"
     gene            11170..11430
                     /locus_tag="PLW22_02370"
     CDS             11170..11430
                     /locus_tag="PLW22_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOU73579.1"
                     /translation="MTKIITFNELRRVKDSLPDGSMQVIADRLNVNVETVRNYFGGTN
                     YKDGGAVGFHLEPGPDGGIVTLDDTTILDIAYEIIAKSKEMA"
     gene            11511..12368
                     /locus_tag="PLW22_02375"
     CDS             11511..12368
                     /locus_tag="PLW22_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU73580.1"
                     /translation="MEELLSKIHRIETKHLSNLHRHLESIFTNVSLPSHNLQHHIRVW
                     LHCRGLLIELHKAGLTITEQLIENAIIACFFHDAGLTKTLDEQHGAEGAELCKQYLSH
                     FPDLTESQKTLILKAVELHDDKSVKQHPVTKPDDMLDLALLVSTADDLDALGFVGVFR
                     YTEIYLKRGISIDQLPRKVLANLRNRFTSFTNAYSSLHRYSDRQRQRYREIMDFFTKL
                     EGEISQGITYQDSAYAVVNTLHEQLVIQSNSIEQTIDYALSTLTASYPLTFFKRLQLE
                     LEVTSAIPI"
     gene            complement(12408..13019)
                     /locus_tag="PLW22_02380"
     CDS             complement(12408..13019)
                     /locus_tag="PLW22_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOU73581.1"
                     /translation="MKIICIGRNYRDHAKEMNADVPEVPVFFIKPDNCLLRNNQPFFY
                     PDFTSDLQYELEVVLRINRLGRSIKQKFAHRYYDAITLGIDFTARDLQRECKAKGLPW
                     EISKAFDYSAPIGTFIPKDELGDLNGLNFRLELNGKVVQQGNTANMIFGYDELISYVS
                     RFITFRTGDLLFTGTPSGVGPVNVGDRLQAYLNDRLMLDFFIK"
     gene            13124..14026
                     /locus_tag="PLW22_02385"
     CDS             13124..14026
                     /locus_tag="PLW22_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOU73582.1"
                     /translation="MADPITKSTRKRILFIVNPISGTRGKESILHIINNTIDNETFEP
                     EILFTRKKGDALQIISEKIAEGFKYFVAVGGDGTVNEVGSAIINTDCVMGIIPLGSGN
                     GLARHLRIPLDAAKAIQVINKLRVESIDYGLVNGLPFFCTCGVGFDAHIGYRFAKEKG
                     RGFFNYVKVALTDFIRYKPKKYKVKIDGTQKMKVKAFLITCANASQYGNNAYIAPNAD
                     IQDGLLDIAIMSPINLLQAPAIGAMLFTRTINKSTVLQTGKAQKVVIKRKREDVVHFD
                     GEPIIMDRKIRITVVNKGLRIIVP"
     gene            complement(14023..14775)
                     /locus_tag="PLW22_02390"
     CDS             complement(14023..14775)
                     /locus_tag="PLW22_02390"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOU73583.1"
                     /translation="MALIETFRRQGDFLFRYRSYLPVLLILAGIGYYVYLLETKQYSY
                     NAFYFFACFVVSFIGLVIRAITIGYTPKNTSGRNTKEQVADTVNTSGMYSLVRHSLYL
                     GNFFMWFGLALLTKSYWFVISFLLVYWLYYERIMYAEEFFLRNKFGSDYLNWADSVPT
                     FIPRRFRWRSPNNYFSIRNVIKRESIGLFKLVLLFFVFQYIHDVVSNGGKLLELSSWG
                     RAWFIAFIIVGVIYLTLRTIRKKTRLLDVDGR"
     gene            complement(14845..15612)
                     /locus_tag="PLW22_02395"
     CDS             complement(14845..15612)
                     /locus_tag="PLW22_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU73584.1"
                     /translation="MGLNIKNPLVVFDLETTGIDVVKDRIVEIAVLKVFPNGNRESKV
                     RRINPEMPIPPEATAVHGITDDDVKDAPTFKEIAKSLANYIEGCDFAGFNSNKFDLPL
                     LAEEFLRAGVDIDLKKRKFIDVQTIFHKMEKRTLAAAYKFYLDKDLVNAHSAEADTLA
                     TFEILCAQVERYPELKNDVDFLSEFSSFNRNVDFAGRIILDDKGVEVFNFGKHKGKPV
                     VEVLKAEPSYYSWMMNGEFPLYTKKVLTNIYLKMKQK"
     gene            complement(15680..16810)
                     /gene="dnaN"
                     /locus_tag="PLW22_02400"
     CDS             complement(15680..16810)
                     /gene="dnaN"
                     /locus_tag="PLW22_02400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU73585.1"
                     /translation="MKFVVSSTMLLSHLSVVSRVISSKNTLPILDNFLFKLEGNELEI
                     TASDLESTLTTKITLDNVLGDGSIAIPFKILIDTLKEFSEQPLTFEIDPGSLAVVIHS
                     ENGQFNIVGQPADDFPKPAELSPESKVSVKVAADVMLNGITKTIFATADDEMRPVMNG
                     VFVELTTEGMTFVASDSHKLVRYRRKDVQVEEFSSFILPKKPANLLKSILVKETGSVN
                     VEFDSKNARFTMSGYTLVCRLVEGEYPNYSAVIPVDNPNRMIIDRVDFYNSVRRVSIY
                     SNAASNLIKLGLTSNQCEVSAQDLDFSVSAYERLNCDYQGDEMEIGFKSAFLVEILAN
                     LSSTEVVLSMSDPSRAGLIFPHQNENPDEDVLMLLMPMMIGA"
     gene            complement(16965..17936)
                     /locus_tag="PLW22_02405"
     CDS             complement(16965..17936)
                     /locus_tag="PLW22_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73586.1"
                     /translation="MSSKKIIFLSLLIALGIGLFYLSRWYKEFRQSDFSFSSSQITAI
                     ELVMKSDTLRLTKSDGNWFINDFFLADSAVVSNFIDILQSISAIAPATLKTDENLIQL
                     FQRKGIGVFIYKGKRVKKEYRIASVAEFNYKTVALNSGSEVPYYVESANAVDNLVEYF
                     SVNPDKWLAGKLFPEPVEAIEEIKAIFPDKAKGYTIELGGNQIALSNSAGNRMPNINL
                     ANISNLYYSLDNFKLHYPTKAEIDSAKPENLISSLQLKMLNGKTYEYTFYRITGKGFT
                     NILGQELGYNPNRLLVKLSENKFLVGSYLHFHYVLCPIDVFVNNYEK"
     gene            complement(17926..19644)
                     /gene="gldG"
                     /locus_tag="PLW22_02410"
     CDS             complement(17926..19644)
                     /gene="gldG"
                     /locus_tag="PLW22_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOU73587.1"
                     /translation="MSVSGKIKIRSIRAFAVLLLILLLVNALSSFFYFRIDLTEDKRF
                     TIQPVSREVLQGLDDIVVIRVYLDGELPVAFVRMKKALAETLSEFQEIAGKKIRYEFV
                     NPYSTSGAKKQEQLFRQLAEKGLVPTNVHVKDNEGAMIQRIIFPGCIITYRGKELAVN
                     LLRNNPNLTGDENINLSIQNFEYALVDAIDRITTEHRQRIAFIQGHGEFDEFETGDIE
                     RELAQYYDVSRVIINGDTDALKPYKTIIIAGPSRPLPEADKLIIDQFIMQGGRVLWFV
                     DPVNVSIDSLSSGASTLAFMNQHNLDDMLFRYGVRLNPLLIQDIQCAVIPVNTALVGQ
                     EPRFVPAPWLYYPLLLPPADNPITKGLNLIQTKFISPIDTVGMDDEVEKRILLFTSKE
                     AKLVKIPALINLNQVSKGITRYEFQHSMLPVAVELQGIFKSNFRNRPINAIIPNGTIK
                     FHDKSIFTKMIVVADADIIRNDIQRRPNGAYVIPLGYDRFTQQTYGNKELVVNMVKYL
                     CDDSGLLDLRSRDVKLRLLNRKRAVSERLYWQLVNMVLPSLMLVLAGIIWSFVRRKKY
                     TFTNVK"
     gene            complement(19664..20395)
                     /gene="gldF"
                     /locus_tag="PLW22_02415"
     CDS             complement(19664..20395)
                     /gene="gldF"
                     /locus_tag="PLW22_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOU73588.1"
                     /translation="MKAIFIRELQNFFSSLTGYVIAIIFLVLTGLFLWVLPGYTNILD
                     SGYATLESLFYIAPWLFLFLVPAATMRMFADEKKSGTIELILTRPISDTAMVLGKFFA
                     SLVLVAIVIVPTFIFVYSVSNLGNPSGNLDWAATWGSYIGLFFLASAYAALGVFASSL
                     TDNMIVAFLLAVALCFVFYDGLAWVAELQSMEWARSALLTLSISEHYSSVSRGVIDSR
                     DVVYFLSISSVFLALTRLKLQSRRW"
     gene            complement(20420..21604)
                     /locus_tag="PLW22_02420"
     CDS             complement(20420..21604)
                     /locus_tag="PLW22_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOU73589.1"
                     /translation="MRVVLYSFLLFLPFLSNSQKLSISVFNDTNLNTILVTPTKGEYS
                     MVLGDSIITIKPLQILYISRQGDSVLVRDENQSYGSFKRVTLIGQTGDDVFRLKPIIP
                     AKPARLYDDNITFYVEFNRIMAVNIIDREKYIAAVVLAETGPNKEPELYKAQALLART
                     FTVTHIDKHQSEGFNLCDQEHCQAYKGRPEAFQEIYDAVLDVKGLIVVDSSLKPISAA
                     FHANCGGQTANSEDVWIQALPYLRSVNDRYCTSAPNARWERTIPLTEWKKFLAQQGVD
                     TAKVTRDNLNFYPKERPKYYTVLGVNIATTAVRKYFKLRSAWFTVSAGKSEVRLSGKG
                     YGHGVGMCQDGALVMAQRGFTCDKIIQFYFKDVQIIDISKVNLDSLKPDTLEVKPFDV
                     LQ"
     gene            complement(21615..22739)
                     /gene="lpxB"
                     /locus_tag="PLW22_02425"
     CDS             complement(21615..22739)
                     /gene="lpxB"
                     /locus_tag="PLW22_02425"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOU73590.1"
                     /translation="MKYFIVAGEASGDLHASNLMKGIKNNDPDADFCFFGGDLMAEQG
                     GTLLKHYRDMAFMGFLEVLLNLRTIKANMELCKQSIIHFRPDVVILVDYPGFNLRIAK
                     FAKLSGFKVFYYIAPKVWAWKESRVEKLKSFVDKLFVIFPFEIDYFRGKGIDPVYEGN
                     PSIDAIANKLPNPISLSDFCEKNGLPQKPIIALVAGSRKQEIRYNLPVMLNVTKRFPE
                     YQFVIAGAPSLDYSVYQPYISGTGVSVVFGQTYQLLSCSTLAFVTSGTATLEAALLNV
                     PQVVCYRGNFFSMLIAWMVIKVKYVSLVNLNMGFEVVRELKQYDLTPDSLYTEAIKLL
                     PGNPERDRQLELYGQLKEKLGKMGASERVGKRIVDLIKSS"
     gene            complement(22746..23036)
                     /locus_tag="PLW22_02430"
     CDS             complement(22746..23036)
                     /locus_tag="PLW22_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73591.1"
                     /translation="MQKKISKQWHGYLLGALLPVITLVVMYLSLASDFKFVDFIRYLN
                     VRGDLSRALSLAVLPNLGLFFVFIWTDRLKAAQGVLGSTIILALIIVFMRFM"
     gene            complement(23017..23844)
                     /gene="surE"
                     /locus_tag="PLW22_02435"
     CDS             complement(23017..23844)
                     /gene="surE"
                     /locus_tag="PLW22_02435"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOU73592.1"
                     /translation="MFEGNGELKRPTILVTNDDGFRAAGIEALVEMVKPFGNVVVVAP
                     EEGHSGMSHAITIKTPLRLKKRKRTDDVEFYSVNGTPVDCVKLAMNQIFTSKLPDLVV
                     SGINHGSNSSISIIYSGTMAAAIEGCLYGVPSIGFSILDYDENPDFTAAIEYGRFIVK
                     NVLENGLTPGSCLNVNVPVLPLKSVKGVKVTRQNKGTWREEFEKRTDPRGVDYYWLTG
                     YFLNEEPNATDTDEYWLNQGYITVVPVHVDLTDYKELDRIKSWRFDQIAISHAEKNK"
     gene            complement(24047..24301)
                     /locus_tag="PLW22_02440"
     CDS             complement(24047..24301)
                     /locus_tag="PLW22_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73593.1"
                     /translation="MENNHTILGIKITDRIKEAGEVQRVLTRYGCSIKTRLGLHEVSD
                     DYCSSSGLIILELTGPADEQAKLHDELVKIPGVITRRMDF"
     gene            24453..27491
                     /locus_tag="PLW22_02445"
     CDS             24453..27491
                     /locus_tag="PLW22_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HOU73594.1"
                     /translation="MNRSYSISILLALTFISQIVYGQYFGRNKPIYKNFKFTVYQTPN
                     FEVYHYLKNDSLLNIISLSAEKWYDRHYQVFRDSIITKNPILLYNDHADFQQTTAISG
                     SVGVGTGGVTEALKNRVIFPLTETWQQTDHVLGHELVHAFQYNSLINGDSTSLNSVRN
                     LPLWMVEGMAEYLSLGTYDYQTAMWMRDAVINDRFPTLEDMTYYPNKYFPYRWGHAFW
                     SFIGRTFGDSLINPIFKETAKRGYNFALMRYLGLNEQSFSTLWKSVYYDYFKRTMPDT
                     VEDPNGTRILFERNAGEINISPSVSPDGRYVAFYSEKDLFSIDLYLAHAISGKIIRRL
                     SSIVHDNEIDALNFIESAGTWSPDSRMFAFVGFSKGKSKLIIVDVFDGKLIQEFTIPG
                     VPSFNYPAWSPDGSSIVVSGLVNGVNDLYQYDLRIKKVDRLTNDPWCNIHPAWSSDGK
                     TIVFSTDKPSQTSANGKTGYNLATLDVETKTINVFDILPGADNVNPMFSANNKEIYFL
                     SNCDGYRNLFRFNIESGEVYRMTRILTGISGMTPLAPAMSVARENDEVVYSHFFKSKY
                     SIYTADSSDFKPVRINPYQVDFNASILPPVNRATEGLVDKNLSDKNLPVVLVDSFKTV
                     PFKPKFKLDYISNIGMGISTSPYGTGMAGGVEMLFSDITGKNMVYTGLSLNGEIYDFG
                     GQVAFLQQKKYITWGVTASHIPYSFGQYGYDTLHRQINGEPVIVEDFQLIQIRMFETA
                     TGLLSYFPLSSTQRFELSGSIARYYYRVDIYSNYYIDGYYYGSERKKGEVPPGFWLQQ
                     SSLAYVYDNSDFGIASPMRGHRMRFQASRTLGEYNYWGALADMRKYIFRKPLSFAFRA
                     LYYGRFGTSINDNILYPLYIGYPWYVRGYDSNSFINYGQSGNVTIDQLTGNHIAVANF
                     EIRVPFTGPERLTLIKSGTLFTELAFFTDAGIAWTKEYPPSLKWKPTSINDRTPFVSS
                     GISLRINLFGMLILEPYYAIPYQLGGFKAANWGINFIPGW"
     gene            complement(27494..28822)
                     /locus_tag="PLW22_02450"
     CDS             complement(27494..28822)
                     /locus_tag="PLW22_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU73595.1"
                     /translation="MKLDISYREIWRVAYPIILGSVAQNILNITDTAFLGRVGEIALG
                     GGVLGGLLYHVLLMLGWGMAMGGQIIVARRVGEGANEAVGRVVEHLLFTLGAFAILIM
                     LFAHALHPAFFSIAVRSNLVCDSASSFFGYRLFGLPFAFLTYSLNAFLIGITRTKVIT
                     LTSFITVFVNIVLDYGLIFGNFGLPQMGVRGAALASVIAEIAGATFLLVYISRMNVSK
                     YKLFNFSSFSKKLLKSILDISWPLMLQFSLSIFVWFVFFLIIEKMGETPLAVSNIVRS
                     IYIILMIPIWGFASATNSFVSQHIGKGKSDQVVKLVVKVMVITLASVLVLSALVLLLG
                     DSIIKIYTNDEHIINATLPVIRVIAIAALTMASAFISFNGVSGTGSTKVSFAIEVFTL
                     TFYLGWAYLIAIILAKSLSLVWTSEVLYGILVTVISIIYLRTGRWKNRVV"
     gene            complement(28889..31015)
                     /locus_tag="PLW22_02455"
     CDS             complement(28889..31015)
                     /locus_tag="PLW22_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU73596.1"
                     /translation="MKRFFLTFFIITLVFTAFGQKELTLDQAVLRQVTEFRPKTLPGF
                     SWLPGTDEYSFVSNDSLIIVNVTDSKKNRAVTLSEVNEALKANSLQPLKRVPSAWGDG
                     KQLRFIAGNYFVLYNSVTKSIASKFLVADNSENHEFSPGGRWMSYTIGNNLFLTNAQG
                     EVITVTNDTTDGIVNGQSVHRNEFGINKGTFWSPSGRYLAYYHMDESMVTKYPLVDIS
                     KRVGELVNIRYPMAGMQSHVVTVRVYDTQSGSTVELKTGEPRDQYLTNIAWTPDERSI
                     LIAVLNREQNHMCMNRYNVSTGDFELTLFEESSSKYVEPLEPPFFINSNSFLWLSRRD
                     GWNHIYLYLADGKFVKQLTKGEWEVTSIDATDFKNGLIYFTSTKESPLDRHLYSVSTK
                     NGSITKLTTSSGTHRCMISPSYKYFVDFYSSITVPNRVALHTIKGKLVKQLIDAPNPY
                     TGYNVSLPNLITLTATDGTTLYGRMIKPVNFDSTQKYPVVIYVYGGPHSQLVTNSWMG
                     GARLWECYMANKGYILFTLDNRGTSNRGAQFEQAIHRQLGTVELGDQLVGVNYLKSLP
                     YVDSTRLGVHGWSFGGFMTITMMLRASDHFKVGVAGGPVTDWKFYEVMYGERYMDTPD
                     ENPDGYKKADLKNYVTNLKGKLLIIHDDMDKTVVPQHSLTLIHEFIKNGVQVDFFMYP
                     QHDHNVMGKDRVHLIDKIIRYFDDNL"
     gene            31191..32099
                     /locus_tag="PLW22_02460"
     CDS             31191..32099
                     /locus_tag="PLW22_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73597.1"
                     /translation="MIETLTMSLRLPKSKLALVLISAFLASCKPSPKQDNETTQPAAD
                     TLSTLVKVENAIFPIPSPWQVVNLIQKNNIPFNESCINSTKNYQRYTTSFKQAVNLGI
                     YGTDLSYLNIYDKTQESLQYLGVIKKLSEQLRITESFDEKIFERIENNIENKDSLIKI
                     ISDTYASSDLYLKKNERYDISALVVAGCWVESLFIMTKIAQEHNDREIINRIGEQKHP
                     LDNLVELLTPFYYKSTEFANLLDSLIDLAYEFDGIIYSYTYREPIVDEANRVITIRSQ
                     SRVVMSEYHLKIISKKIEQIRSSLTE"
     gene            32110..32520
                     /locus_tag="PLW22_02465"
     CDS             32110..32520
                     /locus_tag="PLW22_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73598.1"
                     /translation="MQRILIILTLTLFSLNSFSQIEQLKKTCSINFPSNYISDGQEYF
                     AALKPDQRIEFRTTFYADNTYRISACTNAKKGKLVFTVYDTEKNLLFTNESFNYSPFW
                     DFRFTSTVTCIIQVDVKNPQFIPGYVMLLIGFKQ"
     gene            32534..35635
                     /locus_tag="PLW22_02470"
     CDS             32534..35635
                     /locus_tag="PLW22_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU73599.1"
                     /translation="MSESILKALMQLFAIIARPVSLDEKENNDYADRRKVVEEFLRNQ
                     LNIDLVKEYLKVFDYYYNLYQEKQSEKSKSKKRTSSSSVRVLKICSQINEELQQQQKI
                     VVLIRLLEFVKPDEGEVTEQEMAFISTVAETFYVPEDEFQNLKDFVLSGFDSVIPHQN
                     ILLINDSKEPPYDDLRHLYAEGLHEPIKILFVESANIFFLRYNGLHELYLNGHLLVKD
                     KIFVLNNGSSIRNSKIKPIYFSDIVGRFVQDKIKEKIVFEAIDIEYRFKSGKIGLHPM
                     SFVQESGHLVGIMGASGAGKTTLLNVLNGSEKPTKGSILINGIDIHNQKELVEGQIGF
                     VSQDDLLIEELTVYQNLYFNARLCFDNYTHEQLEAAVNQTLQNLGLYEIKDMRVGSPL
                     NKKISGGQRKRLNIALELIREPAILFLDEPTSGLSSRDSENILDLLKELTLKGKLVFV
                     VIHQPSSEIFKMFDRLLILDTGGYLIYNGDPIESIIYFKSRVQQANWNESECPVCGNV
                     NPEQIFNIVESNVLDEYGNPTRARKISPFEWNEYFKRFAPENPTPNTQPKSLPQIHFK
                     IPNWVKQLRIFVKRDILSKLANSQYMIINIFETPLLALLLAYIIKYYNVDVSNKLGYT
                     LLENSNLPVYLFMSVIVGIFVGLTVSAEEIIKDRKILKREAFLNLSWSSYLMSKVTIL
                     LGISAFQALMFVTIGNSIMEIKGMYFEYWLVLFSTWFSANMMGLVISDSFKTVVTIYI
                     LIPFLVIPQIILSGIIVKYEKLNPTISSPSKIPIYGELIVSRWAYEALAVYQFKENRY
                     MKPLYRFDEAMSLSDYKRNYWLKSIQNKVDFCIRNLNDKEKKAEFKNALELIRNELIK
                     EMTAPRASHLIFSKYTMLTPEEVNADILKELNDFLEQLRKHYVKLYNKASAEKDNYIT
                     RFQNSQPNGQQLFLELKRRYHNESLSEFVRNSGEVERIIEYKNQLIQKIDPIYQFPES
                     KFLRAHFYAPVKPFFGNYFGTIWVNVIVIWLSSILMYLVLYYRLLKRLLEWLESLGGE
                     TES"
     gene            complement(35698..36078)
                     /locus_tag="PLW22_02475"
     CDS             complement(35698..36078)
                     /locus_tag="PLW22_02475"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU73600.1"
                     /translation="MELLNIEGTPKTPTVILNPSTGVIEIKGRSIPENSIEFYRPVVE
                     WLDEYIKNPQPNTVVNVQLEYFNTSSSKCILDVFKKLEVLKKNQKDVIINWYYEEDDE
                     DMLEAGEDYESIIKVPFKMIQIVD"
     gene            complement(36095..36637)
                     /locus_tag="PLW22_02480"
     CDS             complement(36095..36637)
                     /locus_tag="PLW22_02480"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOU73601.1"
                     /translation="MSDFISKCYAKMHSGDVIVAYKGEINANIIADTLGLVEAKLSHE
                     MVENLVRKKLYNVMVECLQNLFHHVDKVTLSENEPRMGAFILSKTNDGYRIATGNLVR
                     NDKSNILKEKIDKINSLSKDELKEFYKFVLNNQTFSEKGGGGLGLIDIARKTGNKLNY
                     EFTPYNDEYSFFNLSVLVSE"
BASE COUNT        11302 a         7786 c         7238 g        10381 t
ORIGIN      
        1 gctcacgagc attgcgagta atccctctct ctccgccaca aagtaggaat gttgtgttcc
       61 tacttttttt tatttatacc gggccgaagg gttcgaaccg agaacggctt tgccgttcga
      121 gctcacgagc attgcgagta atccctctct ctccgccaca aagtaggaat gttgttttcc
      181 tacttttttt tattcatacc gtgccgaagg gttcgaaccg agaacggctt tgccgaatat
      241 ttcaattctt tactctcaaa aaatcaacat aaaaaaagcc gctcctgaat agaacggcaa
      301 aaaaactaat atctaacacc tttacagcaa ctgcatactg tgaagccttg catagtaacc
      361 gttaagttta agcagctcgt cgtgttttcc gcgctcaaca atttcgcctt catgaaggac
      421 acaaattaag tcagccttgc ggatagtaga aagcctgtgt gcaataacaa tggaggtacg
      481 gtgtttcatg aggttatcaa tggcatcctg aaccaacctt tccgactcgg tgtcaagagc
      541 actggttgcc tcatcgagta tcatgatagg tggatttttc agaattgctc tggctatgct
      601 aacccgttgg cgctgtccac cggaaagttt acttccccta tcgcctacat tggtatggta
      661 tccattgggg gtggcaataa taaaatcgtg agcatttgca atttttgccg catttaccac
      721 atcctccatt ttggctccat caactccgaa cgctatatta ttataaaagg tatcgttgaa
      781 cagaattgga tcctggttaa cattacccat cagattcctc aaactggcta tatcgtaatc
      841 cttaatattt actccatcaa tcagaatttc accctcagta acatcccaaa aacggggtat
      901 aaggtcgacc atggtacttt tacccgaccc cgattgccct acaagggcaa cagtcatacc
      961 tttttttatg gtaaggttaa tgtttttaag gacccaatcg tcctggtact taaagcttac
     1021 attgcggaat tcaatatcat gattgaacga ttcaatgatt ttagcaccag cactaacctt
     1081 gatcggattt tcggcattaa gtatggtatc gaccctatca gctgaagcaa gccccttaag
     1141 tacgttatag tatgcatttg aaaacgactt ggcagggtta ataatcatgt aaaatattcc
     1201 aatgtaagca attaatgcct cagggcctaa attgcccttg cctgcaaata ttagattacc
     1261 tccataccac agaacaagaa caaccacaac ggtaccaagg aattcgctga gaggtgaagc
     1321 caaatcgcgc ctacgccaca tctttttcat cagcaaggag taaaagctat tcatgctttc
     1381 gaaacgcgat atcactttgt tttcggcatt aaaagctttt atcactctta atccgcctat
     1441 ggtttcctca atcatggtaa ggataatgcc catcttggtt tgtcctttaa atgaagtttt
     1501 ccgtaggttc ttacccaccc taccaattac acctccggta agaggtaaaa tgagtagaac
     1561 aaaaagggtt agctgtgggc taatgtagat aagaccgatc aggtgtatga ttataatgat
     1621 tggatcgcgg aagaacatct ccaatgaacg aattattgaa acttcaatct catttacgtc
     1681 gttagtcatc ttgctgataa tatctccctt cttttcctcg gtgaagtatc caagaggtaa
     1741 atcgacaacc ttgcggtaga gtaagtttcg catattttgt aacacacctg ttctgatggg
     1801 agccaaatag tataatgaaa ggtatgtaaa gaggttttta aggaatgagg ctactaaaac
     1861 caatacaata acaaatagta acgccgatgc tctgtcgtgt gttttaatta tttcacttaa
     1921 gtagtagtta aggtattgag caaggccctt tgcggaaatc acgaactctg gttttacctc
     1981 aacaagagga acagttccaa aaagaagctg taaaaagggt attaacatgg ccaatgagaa
     2041 cagcgaaaag acagctgcta gcacattggt aaaaacatta atcacaacat accatttgaa
     2101 tggataaagg tatttgagta ttcggataaa tttcttcata gtatgtgttt ataataattg
     2161 caaaagtaac ctatttattc agtgaaatta atttgaatag cctttattgt ctataattaa
     2221 aactatttaa taccaaggta agtgaaagga gtcagctcta atagttccct tttaaccgaa
     2281 tcttttacat tcaggctatt aacaaagttt gtaaaggtgt cagatgtaac ctgctgattt
     2341 gttcgggtaa gcagttttaa agcatcgtaa gggtttgggt aaccctcgcg gcgaagaata
     2401 gtttgaattc cctcagcaat taccatccag ttactttgta ggtctctatt aattgcatcg
     2461 gtgttaacaa ttagcttgtc aagtccatta agtattgatt ttaatgcgat aaaggaatgt
     2521 gcaatgggaa caccaatgtt cctgataacg gttgaatcgg ttaaatcgcg ctgaaggcgc
     2581 gagattggaa gtttgttggc catgtggtca aataaagcat tcgccaatcc taaattgcct
     2641 tcggcattct caaagtctat cggatttacc ttatggggca tggccgatga gcccacctcg
     2701 ccttccttaa ttttttgctt gaagtattcc atggagatgt acatccaaac atcgcggcta
     2761 aagtcaataa gaatgctgtt gatacgccgc aagcaatcga acatggcggc aaggttatcg
     2821 taatgttcta tttgggttgt agtttggctc cggtgcaatc ccaacttgta gttcacaaat
     2881 tgatttgcaa atcctaccca gtcaatactt ggataggctg caatatgtgc attgaaattt
     2941 ccagttgctc ccccaaattt tgctgagtat ggtatgtgct tgagctgctc tagctgaacc
     3001 tcgatgcgtt ccacaaaaac ccgaaactct ttgcccaatc gagttggcga tgctggttga
     3061 ccatgagtat gggctagcat gggaatatcg gcccactggg tactcagctc tttaagcttt
     3121 gccaataact tttcaagcag tgggagatat tcagtcttaa gggaatcgcg gagcattgca
     3181 gggaatgcag tgttatttac atcctgcgat gttaaaccaa agtgtataaa ttccttatac
     3241 gactctaaac cataggcatc aaacctctct cgcagaaagt attctactgc ctttacatcg
     3301 tggttggtaa ccttttctat ttccttaacc ctaagcgcat cggattcacc aaagcttttg
     3361 tagatttcgc gtagtatggt gaattttgat ttatcaaacc cctggagttg gggcaaagga
     3421 atttcacaaa gggcaataaa atattcaagt tcaacccaaa ccctatactt aattagggca
     3481 aactcggaaa agtaacccga cagcgattct gttttttttg cataacggcc atcgattgga
     3541 gaaatggaat gcaaactatg gttcaacatg ctttacaaaa tttgcccaaa tttactaaac
     3601 ataactcaat gttaaaggtt tatgtaagca ggttttttag caatgaacaa taacttttca
     3661 aaattgctac cttagcaaac taatatttag ccatgaaaaa gtacaaggta tcggccatat
     3721 cgtatttaaa tacagcgcct tttgcttatg gattaaagca ttcaccggta ttcaacaaaa
     3781 ttgaactttt atacgattac ccggcagaat gcgcccgaaa acttgccaac aatcaggttg
     3841 atttgagttt actgcctgtt ggtgcattga gcgattttag ttcgtttgat attgtaagca
     3901 attattgcat tggctcaatt ggaaaagtac gttcagtggc acttatgagt aatagtaatt
     3961 taagcgatat taaaagaata tacctcgatt accagtcgcg cacttcggta atccttgtta
     4021 aaattttatg ccagaaagtg tggaatatta caccagactt tattccctta aaaccctcag
     4081 ataattacaa attacttaaa gaatacgagg ctatcgtttt aattggtgat agagtatttg
     4141 aagccgaaaa tcactttgca tatgtagtgg atttatcgaa tgagtggttc aatgcatatg
     4201 gtttaccctt tgtttttgct gtatgggcaa caacaaaacg tttacctaag gaacttattg
     4261 aggaacttaa caccggactt gaacttggtg taaactcaat tgaaaaagcg gtcgacgaat
     4321 ttagccccct ctcaataagt cgtgaagatg ctataaccta cttgaaacaa aacatatcct
     4381 acacccttga tgaggtgaaa tttaaagcaa taaaactttt tcaaacccta tcggccaaac
     4441 tgaactaaca tcaatttttt atatttttac cgatataaaa ccataccatg aaaaccttaa
     4501 gaatactaag actttttata ttgttgcttt tggctggcat acatatacca gcccaagcac
     4561 aggatagcct accggcaagc aaattgattt acaagataaa tataaaagag aatatcatgc
     4621 ctgcggcatg gaggtcggtg aaggttggct ttgaggaagc ccaaaacctc aaagctgata
     4681 tcatactcat acacatgaac acctatggcg gaatggttga tgtagccgac tcaattagaa
     4741 ccaaaatcct gaacagtaaa attcctgtat gggtttttat tgataaccag gccgcatcgg
     4801 caggtgcttt gatttcaatt gcttgcgata gtatttacat gcgaaaaggt ggaagcattg
     4861 gtgcagctac ggtagtagac caatcgggca atgttgtccc cgataaattt caatcgttca
     4921 tgcgtagcac catgcgagca accgctgagg ccaaaggcta cgatacactc atttcaggga
     4981 acgatacgat actaaaatgg cgacgcgatc ccagaatagc tgaggccatg gtagatccct
     5041 caatatacat tgaaggaatt attgattccg gaaaggttct tacattcaca gccgaggagg
     5101 ccataaagca tggctactgc gaaggtaaag ccgaaaatgt aaacgaggtt atggagcaag
     5161 caggaatcaa atcgtattct attgctgagt ttcgtccatc gctactcgat cgcatgattg
     5221 gttttttaac caatccgttt gtatcgggca tacttataat gattattatt ggcggtatct
     5281 actttgaact tcaaactccg ggcgttggct ttccactcgc tgcggcagct atagcagcca
     5341 tcctttactt tgcgccactt tacctcgaag ggctagttga acactgggag attatcatct
     5401 ttgtagttgg tgtaattcta ctaatggttg agatttttgc catacccgga tttggtgtta
     5461 ccggcatttc gggaatagtg cttatggtag caggattaac cttggccatg attgataatg
     5521 agattttccg cgatccggtt aactttaacc tgatgaccat tcttaaacca tttggtatag
     5581 ttgttttggc tgtatttatt gggttgattg gaggcattgc gctaagccgt aaattactaa
     5641 catcccccct atttcctaat ctggccttac aaagcaactt gaacaaagaa gaagggtttg
     5701 ttggcattga tacaaaaatt aagaacatgg ttggtgccga gggtgttgcc attactaccc
     5761 ttcgcccatc gggtaaaatc caaataggaa atagttggtt tgatgccgtt gccgaatttg
     5821 gctacatcga aaaggggagc aaggttaagg tatcaaagga cgagtcgggg caactttacg
     5881 ttgttaaagc atagaattat ggaaagtatt accattcgag actttcagcc tagcgattat
     5941 gaagggttaa gtgagctttg gctgctcacc aaccttggca atcctgctcg tggcgacaac
     6001 ctggaaataa ttcagcaaag cattgacctg ggaggtaagc tgcttgtggc tgtaaattcg
     6061 gatggaatgg tgattggtac aagctggatg acttttgatg gccgaagaat tcacttacac
     6121 cattttggcg tccatcctaa ctaccaaagg caggggatag gaaaaatgct tacccgcgaa
     6181 tcgcttaggt ttgtcaaagc caaaggctat caggtaaagc tcgaggtaca tcgcaataat
     6241 attgcagcca ttaacctata caaacagttc ggctttcaat accttggcga ttacgatgta
     6301 tacattatca gggatttgaa tagcataagc atttaaaagc caacacattc ctatagagga
     6361 acaaaaaatg catcgaattt tttcgatgca ttttttatct taaaaaaacc tattatcaaa
     6421 aaataccttt acccattagc gacttactgg attgttcatc attggtcgtt gagtttccct
     6481 gagcttaagt atctccttaa gtttttcggt ttccatccaa acccttgggc gagtttttag
     6541 gccaacaccg gcacagaaca ggttaatgtt ctgtgagaca attgctgcat ccatggcagc
     6601 ccaagtcatt ttttggctct catcgcctcg ctgaaagcgg gcaacatcgg agacaatcac
     6661 aagaaaaata ggtgctttgg caaagttttg ctgcgaaccg gcaactgctg aacggtaatc
     6721 accactactg attagaacta gttcatgagc cttggcatcg tacaaaaatg ctcccttgct
     6781 atttaccagg taaacatcaa tatcctgagt gttcatggct gttggggcag ttcgttttcc
     6841 ttcggcagga cggttaatac cgtttgcagc ccacagcaaa tcggctattt cctgaaggtc
     6901 taattcctga gtatcaaatt ccctaaccga ggcacggtta gcaaaggctt ccatgatgtt
     6961 tttaccctta accctgctgg gttcgggtag tttggtaatg ctatcgtttt gtccgtttga
     7021 taccaacgat aacaaaactg ccactaagaa tggtaacgtt ttcatatcaa atatttatta
     7081 acgataaatt cgttccatgt taagcgccct tcggtattcc gctgcgtttc tattatgttc
     7141 tgccaatgtt tgggcaaaaa tatgacgtcc agaaaaatcg ggcgaagcgc agaagtaaag
     7201 atagctatgt ttttgcgcgt ttagcacagc atcaatagca ataactgaag gtatccggat
     7261 agggccagga ggtaatccct tgtgtttata ggtattaaaa ggagagtcaa cggctaaatg
     7321 gcgggttaat acccttcgga ttgtaaaatc gccaatggca aacttaaccg ttgggtcagc
     7381 ctggaggggt attcccttcc gtaatcggtt taaataaacc ccagcaatta ttggcatctc
     7441 cgatttaaca ttggtttctt cctcaacaat tgatgccaaa attgctacct caaccggcga
     7501 taatccaatt ccatcggctt tatgaatccg gctctcgttc caaaaacggt tatattcctt
     7561 atacatccta ttgataaatc ctaatgcatg ggtattccag tagaactcgt atgtattagg
     7621 aataaacatt gccataaaag tgctaggagt gaaaccccga ttaaacgcta ctgaatcgga
     7681 agtaaacaga ttaataagcg aaaccgaatc ggcttcaatc tgtgaagaaa tgcgttgtgc
     7741 tagctgttgc caggttcgca tgccagtaaa ggtaaccttt actggttttt gatgtccata
     7801 cttaagcatt ttaaccatgg aggaattact cataccgggc ttaaaagcat agcatccggg
     7861 tttaacagat ttttcgtaat cgtagtattg tgccaccctt ttgaacctgt cgatactttt
     7921 taataccttg tttttatcca ggatatttag aacatcgtta tagtttgaac ctgtgggaat
     7981 atatattaat gtctcctttg agactgctac attggtagca cgaaacgtaa aatagagcct
     8041 taaaagaagc aaaactccaa ccgctaatcc aaccaaggcg ataaccaacc caaactttac
     8101 aaatttcttc atcttcaaaa aagtaatact aaactaaaat aaaaaaaagc atcgaaataa
     8161 ccatgtagtt cgatgctata taatgttaaa tggaaatagc tttacttgag taaagcaact
     8221 gctttcctaa tacgctcaat ggattcctta agcttatcgt ccgatgtagc atacgaaagc
     8281 cttatgcatt ttggtgaacc aaaagccgaa cccggaacaa cggcaacatg tgcatgattc
     8341 agaaggaaca tggcaaggtc atcggaggtt tttatggttt caccgttata ctgcttgcca
     8401 agcagttccg atatttcagg gaaaatgtag aacgcaccct gtggcttatt caacttgaag
     8461 ccagggattg atgagaaacc ttcatacatc aaatcgcgac gacgccgaaa agcctcgcgc
     8521 atttggcggg tgtattcgcc atcggagtta aatgcagcaa tggctgcacg ttgagcaata
     8581 ctgcaaacgc ccgatgtttg ctgaccctga agtttctcac atgctttggc aatccatttg
     8641 gccgatgccg aaaatcctat gcgccaaccg gtcatggcat acccctttga aacgccattg
     8701 ataataacta ctctatcccg aatagaatca aactgagcaa tactctcatg tttgccaata
     8761 aaattgatat gttcataaat ctcatcggaa attacaatga tatttgggtg cttctcaata
     8821 atttgagcaa ttgcctttaa ttcatcgtag gtgtatacac tacccgtagg gttagaaggc
     8881 gaacataaaa gtagggctct ggttttaggt gtaatagcag cctcaagctg tttgggggta
     8941 atcttaaaat cgctctcaag cgaggtttca atcaccacgc ttttcccctc agcaagctta
     9001 accagctcaa catagctaac ccagtatggg gcaggaacaa taacctcatc gtccttatca
     9061 acaatacaca tcaacacgtt tgaaaggcta tgcttagcac ctcccgaaac cacaatttgg
     9121 tcgggctggt actcgaggtt gttttcgcgt ttaagctttc cacaaatagc ttccagtaaa
     9181 tccttgtaac ccgcaaccgg tgtataaaac gaaaagttct ggtcgatggc tttcttggca
     9241 gcatccttta caaagtcggg ggtaaagaaa tcgggttcgc ctacgctaag gctaattaca
     9301 tcgataccct gtgcagcaag ttcgcgcgac tttttcgaca ttgcaatggt ttgcgactcc
     9361 tcaagagctg ctattctggc tgataccttt tccatggttt aacgttttag ggaattccaa
     9421 attgttttac taattttaga gtttcaaaaa taaagtaatt tgtcattcat atacacctaa
     9481 actgtccaaa aacattaaaa tatgtttgcc gaaattctga aaatcacaat acccgcactt
     9541 atggtattcc tggcaggata ccttgccttg cgaactctta tcaagaatga aacccaaagg
     9601 caaaaggcgg aattaatgtt taacaccata aaggtttcac taccgcttcg acttcaggca
     9661 tacgagcgct taatactctt aatggagcgg atttcgcctg aatcaataat tgtaagagtt
     9721 aataaaaaag ggatgcttgc tagcgaccta caaagtgcgt tacttggaaa catcagagcg
     9781 gaatgggagc acaacctttc ccagcagcta tatgttagca atgaggcatg ggagatggtt
     9841 aaaaatgcta aggaaaacat tgttaagcta ataaatattt gcagcgaaaa agttgcacca
     9901 aacgatagca gcttcgattt gagcaaaaaa attcttgaaa cttacgctga acttgagatt
     9961 aatcccacct cgctggccat tgaattttta aagaaagagg taaaagaaat actttaaaac
    10021 cttgtttaat caatttttag caccctttta acagcatgtg aaaaaccggc ttcatcgttt
    10081 accggcacaa cctgaaattt gtcctttagc tcactgggtg cgtttgaaac cacaaaagct
    10141 tcaccggcaa ggttaagcat atcaatatcg ttgtaatcgt tgcctaaggc caacaaatta
    10201 cggggctgaa ttccgccaat atagctacac aaccattgaa tacctccgga ttttgaaacc
    10261 ccttcgggaa aaatttcaag ccaaattgac ttgccatcga gcggggaggt tgctcttata
    10321 acctgaaccc cgtttaactt tttggaaatg gcattgaacc tttcaatatc attgggtaaa
    10381 acggctaaca cctgcgaagc aggtcctgga aatggcacac cagttattaa gggttgagag
    10441 taatccttat agaggctaat tcttcgttca aaatcagagt ttagtttacc agaactgtgg
    10501 tagtggaaac aatgatttaa gggaattggc tcatgaacca taaaatcggc atgcatttcc
    10561 acaagcaggg caattacata ctgaacaact tccggtgaaa gctggttggc atgaataatc
    10621 gacttatcgg accagcgcat aattcctgct ccagaagaga acaaaaggta atcgatggga
    10681 aaatcgtcgg gcaaaacagt tgatgctgaa aatggtgaac gaccagtagc aatggcacgt
    10741 atgatcccca tttcacctaa tcggcatagg gaaaccatat cttcgccgtt aaacttatga
    10801 tcggaacgca ggagtgtgcc atccaaatcg gttaccacca gtttaatcat gaaaagccta
    10861 tattttccgc aaagatatta aggttcctgg aaaagtttaa agccactatg gtatatgtgg
    10921 tttaaaagta atttagagtt taatgattta tttgtaaaac aatgattgtc tttgtgttaa
    10981 cctcgaatca tttaccagaa tagtaatatt ttccactact tcaatattaa aacatccaaa
    11041 aggaaatttc gtataaaaaa ctagaaaata atgtgttttt ttaacgttaa aatttggtta
    11101 aatcatttta cattttgaac ttaatttttt atatttaagg tgtaaaaatc attttaaaac
    11161 cctgtaatta tgactaaaat aatcacattt aatgagctca ggagggttaa agatagcctt
    11221 cctgatggta gcatgcaggt aattgcggac aggttgaacg ttaatgttga aactgtccgc
    11281 aattattttg gtggaaccaa ctacaaggat ggaggtgctg tgggatttca ccttgaaccg
    11341 ggacctgatg gtggaatagt aaccctcgac gataccacaa tactcgatat cgcctatgaa
    11401 attattgcca aatcaaagga gatggcttaa aacaattcta tccaaatcac gtaaaagcag
    11461 gaaaattttt cctgcttttt tatttacttt gcataaacta catacaaacc atggaagagt
    11521 tactctctaa aattcatagg attgagacaa aacacttgtc gaaccttcac cgccaccttg
    11581 agagtatatt cacaaatgtg tcgttaccct cgcacaatct gcaacaccat attagggtgt
    11641 ggttgcattg ccggggttta ctgattgaac tgcataaggc tggactaaca atcactgaac
    11701 agctaattga aaatgcaata attgcctgtt tctttcacga tgctggccta accaaaaccc
    11761 tcgatgagca acatggggct gagggtgccg agctatgcaa gcaatatctc agccatttcc
    11821 cagacctaac ggaatcgcaa aaaacactga ttttaaaagc tgtagagttg cacgatgaca
    11881 aatcggtcaa gcagcacccg gttacaaagc ccgatgacat gctcgacttg gctttactcg
    11941 tatccaccgc cgatgatctt gacgcacttg gatttgttgg cgttttccgg tatactgaaa
    12001 tttacctgaa gcggggcatt agcatcgacc agcttccacg caaagttctg gcaaacctac
    12061 gtaataggtt tacaagtttt acaaatgcat actcatcgct gcaccgttat tccgacaggc
    12121 aaaggcaacg ctaccgggag atcatggact ttttcacaaa acttgaaggt gaaatatcgc
    12181 aaggcattac ctaccaggat agcgcatacg ctgtagtaaa taccttgcat gaacagctgg
    12241 tgattcaatc gaacagcatt gaacaaacca ttgactacgc attaagcaca ctaaccgcta
    12301 gttacccgtt aacctttttc aaaaggttac agttagaact tgaggttacc tcagctatac
    12361 ccatttaagg ttaaagggaa tacgacaata gttttgctgt aaaaccatta cttaataaaa
    12421 aagtcgagca tcagcctgtc gttcaaatag gcttgtagcc tatcgccaac gttaacaggc
    12481 cctacaccgc ttggggtgcc ggtaaaaaga agatcgccag ttctgaaggt tatgaatcgt
    12541 gaaacgtacg aaataagctc atcgtagcca aaaatcatgt ttgcagtatt cccttgctgt
    12601 actacctttc cgtttagctc aagccgaaag tttaagccat ttaaatcgcc cagttcatct
    12661 ttggggataa aagttcctat tggagcagaa tagtcgaacg ctttggatat ttcccagggt
    12721 aatccttttg ctttacactc gcgctgtaag tcacgagcag tgaaatcgat accaagggtg
    12781 atggcatcgt agtaacggtg ggcaaacttt tgcttgatac tccgccctaa cctattaatt
    12841 cgaagcacaa cttctagttc atattgcaaa tcgcttgtaa aatcaggata aaaaaaaggt
    12901 tgattattgc gaagcaaaca attatcgggc ttaataaaga ataccggaac ctcaggcaca
    12961 tcggcattca tttctttagc atgatcccta tagtttctac caatgcaaat aattttcatt
    13021 atgagtaatt ttttatgtca atgaatgagc aaagccaaac aaaggtagtt agtttgatgc
    13081 tattaaaaaa ggttttactt ttgcatgttc aaaagtaagt tccatggctg atcccataac
    13141 aaagtccacc cgcaaaagaa tactttttat tgtaaatccc atttctggaa cacgcggtaa
    13201 ggaaagtata ctgcacatta tcaataatac aattgacaac gagacatttg agcccgaaat
    13261 ccttttcacc cgaaaaaagg gtgatgcttt acagattata agtgaaaaaa ttgctgaggg
    13321 gttcaagtat tttgttgcag ttggaggcga cggaacggtt aacgaggttg gcagcgccat
    13381 tataaacacc gattgcgtaa tgggaattat acctttaggt tcaggcaatg gtcttgcaag
    13441 gcacctaaga atcccgcttg atgcagccaa agcgatacag gtgataaata agcttagggt
    13501 tgaatcaata gattacgggc tcgtaaacgg gttaccattc ttttgtacct gtggcgtggg
    13561 ttttgatgct catattggtt accgctttgc caaagagaaa ggccgaggtt tttttaacta
    13621 tgttaaggtg gcgctaactg actttataag gtacaaaccc aagaagtaca aggttaaaat
    13681 tgatggcaca caaaaaatga aagtgaaggc ctttctcatt acatgtgcca acgcatcgca
    13741 atatggcaat aatgcgtata tagcaccaaa tgccgatata caggatggat tactcgacat
    13801 agccattatg tcgcccataa atctgcttca agcacctgcc attggtgcaa tgctattcac
    13861 ccgaaccatt aataaaagca cggttctaca aaccggtaag gcccaaaagg ttgtaataaa
    13921 gcgaaaacgc gaagatgtgg ttcactttga tggtgaaccc attataatgg atagaaaaat
    13981 taggattaca gtagtgaata aggggttaag gattattgta ccttaacgac catccacatc
    14041 gagcaaacgg gttttctttc tgatagttcg taaagtcagg taaattacgc cgacaataat
    14101 aaaggcaatg aaccatgccc taccccacga acttagctca aggagtttac ccccatttga
    14161 aaccacatcg tgtatgtact gaaaaacaaa aaatagtagc actaacttga aaagccctat
    14221 gctctcgcgc ttaattacat tacggatgga aaagtaattg tttggtgagc gccacctgaa
    14281 cctgcgcgga ataaaagtgg gaacgctatc ggcccaattg aggtaatccg aaccaaactt
    14341 gttgcgtagg aaaaattcct cggcgtacat tattcgttcg tagtagagcc agtaaactaa
    14401 aaggaaagat atgacaaacc agtagctctt agtaagaagg gcaaggccaa accacatgaa
    14461 aaaattacca aggtaaagcg aatggcggac taacgagtac attcctgaag tgttaaccgt
    14521 atcggcaacc tgctccttgg tattccttcc tgaagtattc ttaggagtgt aaccaatggt
    14581 aatagcgcgt ataaccagtc caataaagct caccacaaag caagcaaaaa agtaaaatgc
    14641 attatatgaa tactgcttgg tttccaggag gtaaacatag taacctattc ctgctaaaat
    14701 taaaagcact ggtaaatagc tgcgatacct aaaaagaaag tcgccttggc gacggaatgt
    14761 ttcaatcaat gccatactaa tcaatttata gaatgctggc gtaaatatag catttgttaa
    14821 tataggttaa tactgtgggg caaattattt ttgtttcatt tttaagtaga tgttagtgag
    14881 aacctttttg gtgtacaagg ggaattcacc attcatcatc catgaatagt agctgggttc
    14941 agcctttaat acttccacaa caggcttacc tttgtgcttt ccaaagttaa atacctcaac
    15001 ccctttgtca tcgagaataa tacggcctgc aaaatcgaca ttgcgattaa acgagctgaa
    15061 ctccgaaaga aaatcgacat cgttttttag ctcaggatac cgttcaacct gagcgcatag
    15121 gatttcgaag gtggcaaggg tatcggcttc ggcgctgtgg gcattcacca agtccttatc
    15181 caggtaaaat ttgtatgctg cagccagggt tctcttttcc attttatgga aaatggtttg
    15241 aacatcgata aacttgcgct ttttcaaatc gatatcaacc ccagcacgga ggaactcttc
    15301 ggccaaaaga ggtaaatcga acttgttgga gttgaaacca gcaaaatcgc aaccctcaat
    15361 atagtttgcc aacgattttg ctatctcctt aaaggttggc gcatctttta catcgtcatc
    15421 ggttattcca tgaacagcag tagcctcagg cggtataggc atttcaggat ttatgcgcct
    15481 aacctttgat tcgcggttcc cgttagggaa tacctttagc acagcaattt caactattct
    15541 atccttaaca acatcaattc cggtggtttc caggtcgaat actaccagcg ggttcttaat
    15601 atttaatccc attaaaatta ccttttatta ataaaaaaac ttccccaagc caaggccggg
    15661 gaagttattg agtttattcc taagcaccaa tcatcattgg cattagaagc ataaggacat
    15721 cttcgtcggg gttttcattt tggtgaggga aaatgagccc tgcacgcgag ggatcggaca
    15781 tggaaagtac cacctctgtt gaggaaaggt ttgccagaat ctccaccaaa aaggctgatt
    15841 taaagcctat ctccatttca tcgccttggt aatcgcagtt taagcgctcg taggccgaaa
    15901 cggagaaatc aaggtcttga gctgaaacct cgcactggtt cgatgttaag cctagcttaa
    15961 taaggttgct ggctgcgttt gaatagattg aaacgcgacg aaccgagtta taaaaatcaa
    16021 ccctgtcaat aatcatacgg tttgggttat caacaggaat tacggcgcta tagttggggt
    16081 attccccttc aaccaaacgg caaaccaggg tgtaaccgct catggtaaag cgggcattct
    16141 tactatcaaa ctcaacattt actgagcctg tttcctttac aagaatagac tttagcaggt
    16201 ttgctggttt tttgggaaga atgaacgatg aaaactcttc aacctgaaca tccttacggc
    16261 ggtagcgaac cagcttgtgt gagtcggaag caacaaaggt catcccttcg gtggttagct
    16321 caacaaatac accgttcatc acggggcgca tctcgtcgtc ggcggtggca aaaatggtct
    16381 tggtaatacc attaagcata acgtcggcag ccacctttac actaacttta ctctctgggc
    16441 taagctcagc gggttttgga aaatcatcgg cgggttggcc aacaatgttg aactggccat
    16501 tctccgagtg aataacaact gcaagggaac ctgggtcaat ttcgaaagta aggggttgct
    16561 ccgaaaattc ctttagggta tcgataagaa tcttaaaggg aattgcaatt gaaccatcac
    16621 ccagaacgtt atcgagggta atttttgttg taagtgtcga ttcaaggtca gatgctgtga
    16681 tttccagctc attaccttcc aacttgaata agaagttgtc gagaattggt agggtgttct
    16741 tgctgctgat taccctgctt accaccgata agtggctcag tagcatggtg cttgatacaa
    16801 caaatttcat atctctgtaa tttagttagt tccttttacc taaattgatt atcctttagc
    16861 tgtcaaacag ttaattgtaa attttagttt aaaaactgaa aatcaatgca attcaaaact
    16921 caaaaggatt tcaataatac aaaattttac tctaataaca cacactattt ttcataatta
    16981 tttacaaaca catcgatggg gcatagaaca taatggaagt gcagatagga gccaaccaaa
    17041 aacttgtttt ccgatagctt taccaataga cgatttgggt tgtaaccgag ttcttgtccc
    17101 agaatatttg taaatccttt accggttatc cggtaaaatg tgtattcgta cgttttgccg
    17161 tttagcattt tgagctgcaa gctggaaatt aaattctcag gttttgcaga atctatttcg
    17221 gccttagtag ggtaatgcaa cttaaaatta tcgagtgagt aataaagatt gctaatgtta
    17281 gcaaggttga tgtttggcat tctatttcca gctgagttag ataatgctat ttggtttcca
    17341 ccaagttcaa tggtgtaccc ctttgctttg tcagggaaaa tagccttgat ttcttcaatc
    17401 gcctcaacag gctcgggaaa cagtttgcca gcaagccatt tatcagggtt aaccgaaaag
    17461 tattcaacta aattatcaac tgcatttgcc gactcaacgt agtagggaac ctccgagcca
    17521 ctgttcaggg caacagtttt atagttgaat tcagcaactg aggctattct gtactccttc
    17581 tttacacgct tccctttata aatgaaaacc cctataccct tccgttgaaa cagctggatt
    17641 aaattttcat cggtttttag ggtagctggt gctatagccg atatactctg caaaatatcg
    17701 ataaaattgc ttacaacggc tgaatcagct aaaaagaaat catttataaa ccagttgcca
    17761 tcggatttgg tcaacctaag cgtgtcagat ttcataacca gttcaatagc agttatttga
    17821 gaacttgaaa aggaaaaatc cgattgacgg aactctttat accaacggga aaggtagaat
    17881 agccctatac ctaaggctat gagtagtgaa agaaaaataa tttttttact tgacatttgt
    17941 aaaagtgtat ttcttccgtc taacaaaact ccaaataatt ccagctaaaa caagcataag
    18001 cgatggtaac accatgttaa cgagctgcca gtacaacctt tcactaacag cccgtttcct
    18061 attgagaagt ctaagcttaa catctctcga acgtaaatcg agcagtccac tatcgtcgca
    18121 taagtactta accatgttaa ccaccaactc cttatttccg taggtttgct gagtaaacct
    18181 gtcgtagccc aacgggataa catatgcgcc atttggacga cgctgaatat cgtttcgaat
    18241 gatatcggca tcagctacta caatcatttt ggtaaaaata ctcttgtcat ggaacttgat
    18301 ggtgccattt ggaattattg catttatggg gcgattccga aagttggact tgaaaatccc
    18361 ttgcaactca acggcaacag gaagcattga atgctggaac tcgtaacggg taataccctt
    18421 gcttacctga tttaggttaa ttaatgcagg aattttcaca agtttagctt ccttagatgt
    18481 aaacaaaagt atccgctttt caacctcatc atccatacca acggtatcaa ttgggctaat
    18541 aaatttggtt tgaataaggt ttaaaccctt agtaataggg ttatcggctg ggggtaagag
    18601 caatggatag tacagccagg gtgcgggaac aaaacggggc tcctgtccaa caagggcagt
    18661 atttaccgga ataacagcac attgaatgtc ctgaatcagc aagggattaa ggcgaacgcc
    18721 atagcggaaa agcatatcat caagattatg ctgattcata aaagccaggg tagaagcacc
    18781 agatgataag ctatcgatac taacgttaac ggggtcaacg aaccatagca cacgtccacc
    18841 ctgcataatg aactggtcaa tgatgagctt atcggcctca ggcaatggcc tgctgggtcc
    18901 tgcaataata atagtcttgt agggttttaa agcatctgtg tcgccattta taatcactct
    18961 gctaacatca tagtattgag ccagctcacg ctcaatatcc ccagtttcaa actcgtcaaa
    19021 ctcaccatgt ccctgaataa aagcaatgcg ctgcctgtgt tcggttgtta tacggtcaat
    19081 tgcatcaacc agggcgtact caaagttttg aatggatagg ttgatattct catcgcccgt
    19141 aagatttggg ttattcctta aaagattcac cgctaactcc tttcccctgt atgtaataat
    19201 acaacccggg aaaatgatgc gctgaatcat tgcaccctca ttatccttaa catgaacgtt
    19261 ggttggaaca agtccctttt cggccaactg gcgaaacaat tgctcctgct tttttgcccc
    19321 cgatgtagag tatggattga caaattcata ccgtattttt ttcccggcaa tctcttgaaa
    19381 ttcacttagt gtctccgcaa gggctttttt cattctaaca aaggcaaccg gcagttcccc
    19441 atcgagatac accctgataa ccacaatatc atctaaaccc tgtaatacct cgcggctaac
    19501 aggctgtatg gtaaaccttt tatcttcggt aaggtcgatt cggaagtaaa aaaacgacga
    19561 tagcgcattc actaaaagta gtatgagcag taaaacagca aatgccctga tacttctaat
    19621 ttttattttt ccactaacac tcataacagt ccattttttg gttttaccac ctgcgcgatt
    19681 gaagttttaa acgggtaaga gctaaaaaaa ctgatgatat ggataaaaag taaaccacat
    19741 cgcgcgaatc aataacaccc cggctaactg agctgtaatg ctcacttatg ctaagagtca
    19801 acaatgccga ccgcgcccac tccatggatt gaagctcggc tacccaggct aatccatcgt
    19861 aaaaaacgaa gcataatgca acagccaaaa ggaacgcaac aatcatatta tcggttaaac
    19921 tcgaggcaaa aaccccaagt gctgcatagg ctgatgctaa gaaaaatagt ccgatgtatg
    19981 agccccaggt tgcagcccaa tccagatttc cggaaggatt ccccaaattt gaaacgctat
    20041 aaacaaaaat gaatgtgggt acaataacta tggcaacgag caccaacgac gcaaagaact
    20101 tccccagtac cattgcggta tcggaaatgg gtcgagtaag aattagctca atggtacctg
    20161 atttcttttc atcggcaaac attcgcatag ttgccgcagg cacaagaaaa aggaaaagcc
    20221 atggtgcaat gtagaacaac gactctaatg ttgcataccc tgaatcgagt atgtttgtgt
    20281 aaccaggcag cacccaaaga aacaaaccag ttaaaacaag aaaaataatt gcaataacgt
    20341 atccggttaa cgatgaaaaa aagttctgaa gctctctaat aaaaattgcc ttcatagatg
    20401 cttgtgtttt aggataaaat tactgtaaca catcaaaagg cttaacctca agggtgtcgg
    20461 gctttaggga atcaaggtta accttggaaa tatcaataat ttggacatcc ttaaagtaaa
    20521 actggattat cttatcgcaa gtgaaaccgc gctgggccat aaccaatgcg ccatcctggc
    20581 acattcccac accatgaccg tatcctttac ccgaaagacg aacctctgat tttcctgctg
    20641 aaaccgtaaa ccatgcggaa cgcaacttaa aatacttccg aacggcagtt gtagcgatat
    20701 ttacacctaa aacagtatag tatttaggcc tctccttggg gtaaaagtta aggttatcgc
    20761 gtgttacctt agcggtgtca accccttgct gagcaagaaa ttttttccat tcggtaagag
    20821 gtattgttct ttcccagcga gcgttaggtg ccgatgtgca atacctatcg tttacactcc
    20881 tgaggtaggg caatgcctga atccaaacat cctcggagtt tgctgtttgc ccaccacagt
    20941 ttgcgtgaaa tgcagcgcta attggcttta aggaagaatc aacaacaatt aaacccttta
    21001 catcaagaac tgcatcgtaa atttcttgaa aagcctcagg ccttcccttg tatgcctgac
    21061 agtgctcctg atcgcaaagg ttaaaaccct ccgactggtg tttatcgata tgggtaacag
    21121 taaatgtccg ggcaagtaag gcttgcgcct tatacaattc gggttcctta ttcgggccgg
    21181 tttcagcaag aacaacagct gcaatatact tttcgcgatc aattatgttg accgccataa
    21241 tacgattaaa ctcaacgtaa aaggtaatat tatcgtcgta taaacgagcc ggttttgcag
    21301 gaattattgg cttaaggcga aaaacatcat cgccggtttg gccaataagc gtgacccgct
    21361 tgaatgaacc atagctttgg ttctcgtcgc gcaccagaac agaatcgcct tgacggctaa
    21421 tgtacagtat ttgtaatggc ttaattgtaa tgatactatc gccaagtacc atgctgtact
    21481 cacccttagt gggtgtaaca agtattgtat ttaaattagt atcgttaaaa acgcttatgc
    21541 ttaacttttg cgagttcgat aggaatggca gaaaaagcaa aaacgaatac agaactactc
    21601 tcataagcaa gtatttaaga tgattttatc aaatccacaa tccttttacc cacccgttcc
    21661 gatgcaccca tcttaccaag tttctccttg agctggccat aaagttccag ctgacgatct
    21721 cgttccggat tgccgggtaa aagttttatg gcctcggtgt aaagactatc tggtgttaaa
    21781 tcgtactgtt tcaactccct aacaacctca aaccccatgt tcaggttcac cagtgagaca
    21841 tacttcacct taataaccat ccaggcaatt agcatggaga agaagttccc cctataacaa
    21901 accacctgtg gaacgttcag tagcgccgcc tcaagcgttg ctgtcccgga agttacaaag
    21961 gcaagtgttg agcatgagag caactggtag gtttgaccaa acaccacact tacaccagta
    22021 ccactaatat aaggctggta aacactataa tccaacgatg gggcaccggc tattacaaat
    22081 tgatactcgg gaaacctttt ggttacattc aacatcacag gcaaattgta gcgtatttcc
    22141 tgctttcggc tacctgcaac aagtgctatt attggttttt gaggtaatcc atttttttca
    22201 caaaaatcac ttaaagaaat tggattagga agtttgttag caatggcatc aattgaaggg
    22261 ttaccctcgt aaacggggtc aatgccttta cccctgaaat aatcaatctc aaaagggaaa
    22321 atcacaaaaa gtttatcgac aaagcttttc agtttctcaa cacgcgattc cttccaagcc
    22381 caaacctttg gcgctatata gtaaaacacc ttaaatccgg atagttttgc aaatttagca
    22441 atacgtaagt taaaaccggg ataatccaca agtattacta catctggcct aaagtgaata
    22501 atactttgtt tacagagctc catgttagcc tttatggttc gtaggttaag taaaacctcc
    22561 aaaaaaccca taaaggccat atcgcggtaa tgctttaaaa gggtaccacc ctgctcagcc
    22621 attaaatcgc caccaaagaa acagaaatcg gcatcaggat cattattttt aatacccttc
    22681 atcagatttg atgcatgtaa atcgcccgat gcttcgcctg caactataaa gtatttcatt
    22741 ttaatctaca taaaacgcat gaaaacaatt attaaagcaa gaattatggt gctacccagt
    22801 acaccctgag cggccttgag cctatcggtc caaataaata caaaaaacag gccaaggttg
    22861 ggtaaaacgg caagcgataa tgctctggac aaatcgcctc gtacgtttaa atatctaata
    22921 aaatcgacaa acttaaaatc gctggcaagc gataaataca taactaccag cgtgatgacg
    22981 ggcagcaaag ctccaagtag gtatccatgc cactgtttac ttattttttt ctgcatgcga
    23041 aattgcaatt tggtcaaacc gccacgattt tatacgatcg agttctttgt aatcggtcaa
    23101 atcaacatga actggcacaa ctgtgatata cccctggttt agccagtact catcggtatc
    23161 ggttgcgttg ggttcctcgt tcaggaagta tccggttaac cagtagtaat caacacctct
    23221 gggatcggtt cgtttttcaa actcctcgcg ccaggtgcct ttattttgtc gtgtaacctt
    23281 tacccctttt actgacttta acggcaaaac aggaacattc acattgagac acgagcctgg
    23341 agttaaccca ttttcaagta cgtttttaac tataaacctt ccatactcaa tagcagcagt
    23401 aaagtcgggg ttttcgtcgt aatcaagaat agaaaaacca atggaaggta caccgtacaa
    23461 gcatccctct atggctgcag ccatggtgcc actgtatatt atgctaatgg atgagtttga
    23521 gccatggtta atacccgata ctaccaagtc gggcaactta cttgtaaaaa tctgattcat
    23581 ggcaagtttc acgcaatcca caggagtacc gttaacggag tagaattcaa catcatcggt
    23641 acgtttacgt ttttttaacc tcaagggagt tttaatggtt atagcatgcg acatgccgga
    23701 atgcccttcc tccggagcaa caaccacaac attcccaaag ggttttacca tttcaacaag
    23761 tgcttcaata ccggcagccc tgaatccatc atcgttggtt acaagtattg ttggtctttt
    23821 caactcaccg tttccctcaa acatcctttt ctgatttgtg ttaattggca aagatataat
    23881 tttttagaca gtaaaactcc tcagccggca gggttgtttt gacttttgca aatattaacg
    23941 tcaagcaata attctttgtt gtttacctca atcatgcatc aaagcaataa aaaagttcgg
    24001 ccgattgttc ctgtggccga actcaattaa gtattaagtc aaaacactaa aaatccattc
    24061 ttcgagtaat tactccgggt attttaacca gttcgtcgtg taattttgct tgttcatcgg
    24121 cggggccagt aagctcaagt attattaatc ccgaggaact gcagtagtcg tcagaaacct
    24181 cgtgtaaacc caaacgggtt ttaattgagc acccgtaacg ggttaaaacc cgttgaacct
    24241 cgccggcctc cttaattcta tcggtaattt taatacccaa aattgtgtgg ttgttttcca
    24301 tagctaacag ggtttttggt ttccataaat ttaggttaaa aattgaaaca tttcaagtga
    24361 gatttggtta aatttatgtg atttaggtgc aatttttgtg gtaaaaaact taccttgcag
    24421 actcactgat taaaactaaa agcttaaata aaatgaatag aagttattct atttcaatcc
    24481 ttttggcact tactttcatt agtcaaattg tttatggcca atactttggc agaaataaac
    24541 ccatctacaa gaattttaag tttaccgttt atcaaacccc aaattttgag gtttaccatt
    24601 acctgaaaaa cgatagtcta cttaatatca tatcgctttc agccgaaaag tggtacgacc
    24661 ggcactacca ggttttcagg gattccataa taacaaaaaa cccaatactg ctctataacg
    24721 atcatgccga tttccagcaa accacggcta tatcggggag cgttggagtg ggaactggtg
    24781 gtgtaacaga ggccctgaaa aaccgtgtaa tcttcccatt aacggaaacg tggcagcaaa
    24841 ccgatcatgt ccttgggcac gagcttgtgc atgcttttca gtataactct ttgattaatg
    24901 gcgattccac ctcgctcaac tcagttcgga accttccact ctggatggta gagggcatgg
    24961 ccgagtacct atcgctaggc acatacgact accaaactgc aatgtggatg cgcgatgctg
    25021 taattaacga caggtttccc acccttgagg atatgaccta ctaccccaac aagtacttcc
    25081 cttaccgctg gggacacgca ttctggtcgt ttattgggcg aacattcggc gatagcctta
    25141 taaacccaat attcaaggaa accgcaaagc ggggctacaa ctttgcactc atgcgttacc
    25201 ttgggttgaa cgagcaatcg ttctcaactc tctggaaatc ggtatactat gactatttta
    25261 agcgtaccat gcccgatacg gttgaagatc ctaacggaac ccgaatcctt tttgaacgaa
    25321 atgcaggcga aataaacatt tcgccatcgg ttagccccga tgggcgatat gtggcttttt
    25381 actccgaaaa ggacctcttt tctattgacc tttacctggc acatgccata agcggtaaaa
    25441 ttattagaag gttatcgagc atagtgcacg ataatgagat agatgcactc aactttattg
    25501 agtcggccgg gacatggtcg cccgatagcc gaatgtttgc ctttgtagga tttagcaagg
    25561 gtaaaagcaa attgattatt gttgatgttt ttgatggtaa actcattcag gagttcacca
    25621 tacccggtgt tccatcgttc aactatcccg cctggtcgcc cgatggtagt agcattgtgg
    25681 tgtcaggact tgtaaatggg gttaacgatt tataccagta cgatttaaga attaaaaagg
    25741 ttgaccgcct caccaacgat ccctggtgca atatacatcc tgcatggtcg agcgatggca
    25801 aaaccattgt tttttcaacc gataaaccct cacaaacatc agctaacgga aaaacagggt
    25861 ataaccttgc cacattagat gtagagacta aaaccattaa tgttttcgat attctccccg
    25921 gtgccgataa tgtaaaccca atgttttcag ccaacaacaa ggaaatttac tttctttcaa
    25981 actgcgatgg ataccgtaac cttttcaggt ttaacattga aagcggcgaa gtttacagga
    26041 tgacaagaat tttaaccggc atctcgggaa tgacaccgct tgcaccggcc atgagcgtag
    26101 cccgtgagaa cgatgaggtg gtttactccc atttcttcaa aagcaagtac tccatatata
    26161 ctgccgatag ctccgatttt aaaccggtta gaataaatcc atatcaagtt gattttaacg
    26221 catctatcct tcctccagta aatagggcaa ctgaaggttt ggttgataaa aatctctcgg
    26281 ataaaaacct acctgtggtc ttggtcgatt catttaaaac cgtaccgttt aagcccaagt
    26341 ttaagctcga ttatatttcc aatattggca tgggtataag tacctcacca tacggaacag
    26401 gtatggctgg cggggtagaa atgcttttta gcgatatcac tggcaaaaac atggtgtata
    26461 ccggattgtc gcttaacggc gaaatatatg attttggagg gcaagtagca ttcctccagc
    26521 aaaaaaagta catcacttgg ggtgtaacag cttcccatat cccctactct ttcggacaat
    26581 acgggtacga tactctccac aggcaaataa atggcgagcc tgtaatagtt gaagattttc
    26641 agctcattca gatacgaatg ttcgaaaccg ccacgggact actttcatat tttcccttat
    26701 catccacaca gcggtttgaa ctaagtgggt caatagcccg ctactattac agagtcgata
    26761 tctactcaaa ctattacatt gatggctact actacggttc cgaacgcaag aaaggggaag
    26821 taccaccggg cttttggtta caacaatcga gtttagccta tgtatacgat aactccgact
    26881 ttggtatagc ctcgcccatg cgtgggcaca ggatgcggtt ccaggcttca agaacccttg
    26941 gggaatataa ctactggggc gccttagccg acatgcgtaa atatatattc cgtaaaccgc
    27001 ttagcttcgc tttccgcgcc ctgtactacg gtcgttttgg cacatcaata aacgataaca
    27061 tactataccc gctatacatt ggctaccctt ggtatgttag ggggtacgat agcaattcat
    27121 ttattaacta tggccaatcg ggcaatgtaa ccattgacca gctaaccgga aaccatatag
    27181 cggttgctaa ttttgaaata cgagttcctt ttacaggccc tgagcgccta accttaatta
    27241 agtcgggtac attgtttacg gaactggcat tctttaccga tgcaggtata gcatggacaa
    27301 aggagtaccc gccaagctta aaatggaaac ccacctccat aaacgaccga acgccttttg
    27361 taagttcagg gattagcctt aggataaatc tgttcggaat gcttattctg gaaccttact
    27421 atgcaatacc ctaccagcta ggcggattca aggcggcaaa ctggggtatc aactttattc
    27481 ccgggtggta gtgctaaacc acccggtttt tccatcggcc agttcttaag taaataattg
    27541 agattactgt aaccagaata ccatatagca cctccgaagt ccaaactaag cttaaggatt
    27601 tagctaaaat tattgctata aggtaagccc agcctaagta aaatgtcagt gtaaacacct
    27661 caatggcaaa cgatacctta gtgctaccgg tacctgaaac accattgaaa ctgataaagg
    27721 ccgaagccat tgttaaagca gctattgcga ttaccctaat aacaggcagg gtggcattga
    27781 ttatgtgctc atcgttagtg taaatcttaa taatgctatc tcccagcaaa agaaccagag
    27841 ccgaaaggac aagaactgag gctaaagtaa tgaccattac ttttacaacc agctttacca
    27901 cctgatccga cttacctttt ccaatatgtt gacttacaaa tgaattggta gccgaagcaa
    27961 atccccaaat tggtatcata aggataatgt aaatgcttcg aactatgttg gagacagcaa
    28021 gcggggtttc acccattttt tcaataataa ggaagaatac aaaccataca aaaattgata
    28081 gtgaaaattg aagcatcaag ggccaggaaa tatcaagtat tgacttcaat aacttttttg
    28141 aaaacgacga aaagttgaat agcttatact tacttacatt cattctgctt atgtaaacaa
    28201 gtaaaaatgt agcccctgca atttcggcaa taaccgatgc tagggctgcc cctctaacac
    28261 ccatttgtgg tagtccaaag ttaccgaata tcaatccgta atcgagaaca atgtttacaa
    28321 atactgtaat gaacgatgta agtgtaatca cctttgtacg tgtaattcct attaggaatg
    28381 cgttcaatga gtatgtgaga aaagcaaagg gtagcccaaa tagcctatac ccaaaaaaag
    28441 aacttgcaga atcgcataca agatttgacc taacagcaat actaaaaaaa gccggatgaa
    28501 gagcatgtgc aaatagcatt ataagaatag caaaggcgcc gagagtaaaa agtaaatgct
    28561 caactacacg gccaacagcc tcatttgccc cctcgcctac acgccttgcc acaataattt
    28621 ggccacccat agccattccc cagcccaaca taagcaaaac atggtaaagc agccccccaa
    28681 gaacacctcc acccaatgca atctcaccaa ctcgtcccag aaaagccgta tcggttatgt
    28741 tcagaatatt ttgggcaacg cttcctaaaa ttatgggata tgctaccctc cagatttccc
    28801 tgtaggaaat atcgagtttc attggaaaat atttgtgtaa aaatacaaaa aggcagggga
    28861 ttccctgcct tttattgagc ccttagcact ataaattatc gtcgaagtat cgtataattt
    28921 tatcaatcaa atgcacccta tccttaccca ttacattatg gtcgtgttgg ggatacataa
    28981 agaaatcaac ctgtacgcca tttttaataa actcatgaat taaggtgagg ctatgttgcg
    29041 gaactaccgt tttatccata tcgtcatgga taataagaag cttgcctttc aggtttgtta
    29101 catagttctt aaggtcggct tttttgtatc catctggatt ctcatcggga gtatccatgt
    29161 aacgctcacc atacatcacc tcatagaatt tccagtcagt aaccggacca ccagcaacac
    29221 caaccttaaa gtggtcggat gccctaagca tcatggttat tgtcataaaa ccgccaaaac
    29281 tccacccatg aacccctaac cgggttgaat caacataagg aagtgatttt aagtaattaa
    29341 ctcctacaag ttgatcgcca agttcaaccg tacccaattg cctatgaata gcttgctcaa
    29401 attgagcgcc acggttcgaa gtgccacgat tatcgagtgt gaaaagaata tagcccttgt
    29461 tagccatgta gcactcccaa agccttgcgc ctcccatcca gctattggtt accagctgcg
    29521 aatgtggtcc cccataaacg tatattacaa ctgggtactt ttgtgtagaa tcgaaattta
    29581 ctggttttat cattcttcca tataaagtag ttccatcagt tgcggtaaga gtgattaggt
    29641 ttggcaacga aacattatag ccagtgtatg ggtttggcgc atcaattaac tgttttacca
    29701 gtttaccctt aatggtgtgc aatgcaaccc gattgggaac tgttattgag ctataaaaat
    29761 caacaaaata cttatagctt ggactaatca tgcagcggtg agtccctgag ctcgttgtca
    29821 actttgtaat agagccattt ttagttgaaa cagagtataa atgcctatcc aatgggctct
    29881 ccttggttga cgtaaagtaa atcaagccat tcttaaaatc ggtggcatct atacttgtaa
    29941 cctcccattc ccctttggtg agctgtttaa caaacttacc atcagccaaa tacaggtaaa
    30001 tatgattcca tccatctcga cggctaagcc acaaaaatga gttgctgtta atgaaaaaag
    30061 gcggttcaag tggttcaaca tacttactac tgctctcctc aaacagggtt aactcaaaat
    30121 cgccagtgct tacgttgtaa cgattcatgc acatgtggtt ttgttcccta ttcagaacag
    30181 caatcagtat ggaacgctca tccggagtcc atgcaatatt ggtcaaatac tgatcacggg
    30241 gttccccggt ttttagttca accgttgaac cgctttgagt atcataaacc ctaacggtta
    30301 ccacatggct ttgcatgcca gccataggat acctaatgtt caccaactcg ccaacacgct
    30361 ttgaaatatc aaccaatgga tatttggtaa ccatactctc atccatgtgg taatatgcca
    30421 ggtatcgtcc agaaggcgac caaaacgtac ccttgttaat tccaaactca tttcggtgaa
    30481 cactctgccc gttaacaatt ccatcagtag tatcgtttgt tactgtaata acctcaccct
    30541 gagcatttgt aaggaaaagg ttattaccaa tggtatacga catccacctg ccgccagggc
    30601 taaactcgtg gttttcagaa ttatcggcca caagaaactt tgacgcaatg cttttggtta
    30661 cagagttata caatacaaag tagttacctg ctatgaagcg gagctgcttg ccatcgcccc
    30721 aagccgatgg tacccttttt agaggctgga ggctattggc ctttagagcc tcgtttactt
    30781 ccgataaggt tacagctctg tttttctttg aatcagtaac attgacaatg attaagctgt
    30841 cgttgctaac aaaggaatac tcatcggttc caggcaacca gctaaaaccg ggtagggttt
    30901 tagggcgaaa ctccgtaacc tggcgtaaca cagcctgatc cagggtaagt tccttttgcc
    30961 caaaagctgt aaataccagt gtaataataa aaaaggttaa aaaaaatctt ttcatatcat
    31021 aaattttagg gattgcacta ttgaaattta agctaaaata gttaacttta ttgtttaaat
    31081 tgatactaaa aagtttgtat ggtttaaaaa gtaaagcaga aacctacata tttcaacatt
    31141 tgtggtattt ttgcttagta aacatttcgt taaaaagcta tttttctttt atgattgaaa
    31201 cactgacaat gagcttacga ttacccaaat caaaattagc cctagtactt ataagcgctt
    31261 ttttagcatc ctgtaaaccc tcacccaaac aggataacga aacaacccaa cccgcagccg
    31321 atacgctatc gacactggtt aaggttgaaa atgccatttt cccaattcct tcgccctggc
    31381 aggtggttaa ccttattcaa aagaacaata tcccattcaa tgaatcatgc attaatagta
    31441 caaaaaatta tcagcgctac actacctctt ttaagcaagc ggtaaacctt ggtatttatg
    31501 gtaccgactt aagctaccta aacatatacg ataaaactca ggagtcgctc caatacctag
    31561 gtgtaataaa aaagttaagc gagcagctaa gaatcactga atcgttcgac gaaaagattt
    31621 ttgaaaggat tgagaacaat atcgaaaata aagactcact gataaaaata atatcggata
    31681 cctacgcaag ttctgatttg tacctgaaaa agaatgaacg ttacgatata agtgcattgg
    31741 ttgtagctgg ttgttgggtg gaaagcctgt tcattatgac aaaaattgcc caggagcaca
    31801 atgatagaga gattattaac cgtattggag aacaaaaaca cccactcgat aacctggttg
    31861 agctgttaac gccgttctac tataaatcaa ccgaatttgc caatttgctc gacagcctta
    31921 ttgaccttgc ctatgagttt gatgggataa tctactccta cacataccgc gaaccaattg
    31981 ttgacgaggc aaatagggtt ataaccatta gaagtcaatc gcgggtggta atgtcagaat
    32041 atcaccttaa aattatcagc aaaaagattg agcaaatacg ctcttcgcta accgaataaa
    32101 tattccaata tgcagcgcat tttaatcata ttaaccctaa cacttttcag cctaaactca
    32161 ttctcacaga ttgagcagct aaaaaaaaca tgctctatca atttcccatc gaactacata
    32221 agcgatgggc aggaatactt tgcagccctt aaacctgacc agcggattga gtttagaaca
    32281 accttttatg ccgataatac ttatagaata tcggcctgca ccaatgccaa aaagggaaaa
    32341 ctggttttta ccgtttacga taccgaaaag aacctactgt ttaccaatga aagctttaac
    32401 tactcccctt tctgggattt tcgctttaca tcaacagtaa cctgcataat tcaggtagat
    32461 gttaaaaatc cccaattcat tccaggctac gtaatgctac taataggctt taagcagtaa
    32521 attcctgtaa accatgagcg agtcgattct aaaagctctc atgcaacttt ttgcaattat
    32581 tgcccgcccg gtttccctgg acgagaagga gaataacgat tatgccgacc ggcgaaaggt
    32641 tgtggaagag ttcttgagga atcagcttaa tatcgacctg gttaaagagt accttaaggt
    32701 ttttgactac tactataacc tttaccagga aaagcaaagc gagaaaagca agtcaaaaaa
    32761 gagaacatca tccagctcgg ttagggttct taagatatgc tcccaaataa atgaggaact
    32821 acagcaacaa caaaaaattg ttgtactaat caggctactt gaatttgtaa aacctgacga
    32881 aggcgaggtt actgaacagg aaatggcttt cattagcact gtggctgaaa ccttttacgt
    32941 tcctgaggac gagtttcaaa accttaagga ttttgtgctc tctggtttcg attcagtaat
    33001 cccgcaccag aatatcctgc ttataaacga ctctaaagag cctccctatg acgatttgag
    33061 gcacctttac gccgaaggcc ttcatgaacc cataaaaatt ctttttgttg agtcggcaaa
    33121 tatctttttc ctacgctaca atggcttaca cgagctttac ctaaatgggc acttgctggt
    33181 taaggataaa atttttgttt taaataacgg ttcatcaatc cgaaattcaa agataaagcc
    33241 aatatacttt agcgatattg ttggccgctt tgttcaggat aagattaaag agaaaatcgt
    33301 ttttgaagcc atcgatatcg aataccgatt caaaagcgga aagattgggt tgcaccccat
    33361 gtcgtttgtt caggagagtg gccacttggt tgggataatg ggcgcatcgg gcgccggtaa
    33421 aaccactcta ctcaacgttt tgaacgggag cgagaagcca acaaaaggga gtatcctaat
    33481 taatggcatt gacatacaca accaaaagga actggttgaa ggacaaatag gatttgtttc
    33541 gcaggacgat ttactgattg aggagctaac cgtttaccag aacctatact ttaatgcccg
    33601 cctttgcttt gataactaca cccatgagca gctcgaggct gctgtaaacc aaaccctaca
    33661 aaaccttggg ctatatgaaa tcaaggatat gcgggtgggt tcacccctaa acaaaaagat
    33721 tagcggtggg caacgcaagc gacttaacat tgcgctggaa ctcatcaggg aaccggcaat
    33781 ccttttcctc gatgaaccta cctcaggatt aagttcaagg gattccgaga acattcttga
    33841 cctcctaaag gaactaacgc ttaaaggcaa gctggttttt gtggttatcc accagccctc
    33901 gtcggaaatt tttaagatgt ttgacaggtt acttatactg gatacaggtg gataccttat
    33961 ttacaatggc gatcccattg aatccattat ctacttcaaa tcacgagtac agcaggccaa
    34021 ctggaacgag agcgagtgcc cagtttgcgg taacgtaaac ccggaacaaa ttttcaacat
    34081 agttgaatcg aatgtgctcg atgagtacgg aaacccaacc agagctcgaa agataagccc
    34141 ttttgagtgg aacgagtact ttaaacgatt tgcccctgaa aaccctaccc ctaacacaca
    34201 accaaagtca ctccctcaaa ttcactttaa aattcctaac tgggttaagc agcttcgaat
    34261 ttttgtaaag cgcgacattt taagcaagct ggccaatagc cagtatatga taattaatat
    34321 ctttgaaacg cctctacttg ctttactatt agcctatatc ataaagtact acaacgttga
    34381 cgtatcaaat aaacttggct atacgctcct agaaaacagc aacttaccgg tttacctatt
    34441 catgtcagtt atcgtaggaa tttttgtggg gttaaccgta agcgctgagg agataataaa
    34501 agatagaaag attctgaaac gtgaagcgtt cctgaacctg agctggtcga gctaccttat
    34561 gtcaaaagtt accatacttc taggcatatc agcctttcaa gccctgatgt ttgtaactat
    34621 tggcaacagt ataatggaaa ttaaagggat gtactttgag tactggctag ttcttttcag
    34681 cacatggttt tcagcaaaca tgatggggtt agtaatatcg gatagcttta aaacggtagt
    34741 taccatatat attcttatac ctttccttgt aataccacaa ataatactta gcggtatcat
    34801 tgttaagtat gagaaactaa acccaaccat ctcctcgcca agtaaaatac ctatttacgg
    34861 ggaactaata gtatcccgat gggcctatga agccttggca gtataccagt tcaaggaaaa
    34921 cagatacatg aaaccgcttt accgtttcga tgaagcaatg agcttatcgg actacaagcg
    34981 taactattgg ctaaaatcaa ttcagaataa ggttgatttc tgtatccgaa accttaacga
    35041 taaggaaaag aaagccgaat tcaaaaatgc tctggagctt attagaaatg aactaataaa
    35101 ggagatgact gctcccaggg cctcacatct tatttttagc aagtacacca tgctaacccc
    35161 ggaggaggtt aatgctgata ttcttaaaga gcttaacgat tttcttgagc agctccgaaa
    35221 gcactacgtt aagctgtata acaaagcaag tgcggaaaag gataactata ttacaaggtt
    35281 ccagaatagc caacccaacg gacaacagct attcttagag ctaaaaaggc gttatcacaa
    35341 cgaaagttta agtgagtttg tacgaaactc aggcgaggtt gagcgaatca ttgagtataa
    35401 gaatcaacta atccagaaga tagaccccat ataccagttc cccgaatcaa aatttctacg
    35461 tgcacacttt tacgccccgg taaaaccttt ctttggtaac tatttcggta ctatttgggt
    35521 taatgtaatt gtgatttggc tatcgagcat tctgatgtac ttggtactct actaccgact
    35581 cctcaaacgc ttacttgagt ggctggaaag tttgggtggt gaaacggaaa gttgatgagc
    35641 ttgatatgaa aaaaggggca aaataatttg ccccttactt tttatactaa ctgctgatta
    35701 atccactatc tgaatcatct taaagggaac tttgatgatc gactcgtaat cctccccagc
    35761 ttcaagcata tcctcatcat cctcctcgta gtaccagtta ataattacat ctttctggtt
    35821 tttctttaga acctcaagct ttttaaaaac atcaagaata cactttgatg aggaagtgtt
    35881 gaaatactcc aactgaacgt taacaactgt atttggctgg ggatttttta tgtattcatc
    35941 gagccattcc acaacaggac gatagaattc tattgaattc tcagggattg atcgaccctt
    36001 tatttcaatt actccggtac tggggttaag tataacggtc ggagtttttg gcgttccctc
    36061 aatgttcagt aattccataa tattgtagtt ttaattattc acttaccaat acactcaaat
    36121 taaaaaaaga gtactcatcg ttatatggag taaactcgta attcagcttg ttacccgttt
    36181 tcctggcaat atcaataagg cctaaccccc caccaccttt ttcagaaaac gtctgattat
    36241 tcagaacaaa tttataaaat tcttttaact catcctttga aagagaatta atcttgtcaa
    36301 ttttttcttt aaggatatta cttttatcgt tacgtacaag gttaccggtg gcaattctat
    36361 acccatcatt ggttttggaa aggataaaag cacccattct gggctcattc tctgagagcg
    36421 ttactttatc aacatgatga aagagatttt ggaggcactc caccatcaca ttgtaaagtt
    36481 ttttccgaac caaattttca accatttcgt gcgataactt tgcttctacc aatccaagtg
    36541 tatcggcaat gatatttgca ttaatctcac ccttataggc tacaattaca tcaccactat
    36601 gcattttggc atagcatttt gaaataaaat cgctcatcga atggtttgta cttgattcaa
    36661 gcaacaccta tgtaatttat atttaaacaa atataataaa attttaa
//
LOCUS       DAOOWJ010000013        36079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1209_length_36079_cov_7.890018, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000013 DAOOWJ010000000
VERSION     DAOOWJ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 36079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36079
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_36079_cov_7.890018"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PLW22_02485"
     CDS             <1..165
                     /locus_tag="PLW22_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOU73602.1"
                     /translation="ELRRIPYLLIVGEKEQEANLVAVRVQGEGDKGQMKLEEFVNHIK
                     AEIDNQLKEI"
     gene            210..746
                     /gene="infC"
                     /locus_tag="PLW22_02490"
     CDS             210..746
                     /gene="infC"
                     /locus_tag="PLW22_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOU73603.1"
                     /translation="MNKKSDIDTYPINEEIRAKTVRLVGENIENPGIYPISQARQMAE
                     EMELDLVMISDKADPPVCKILDYQKFLYQLKKKQKEIKANAQKIVVKEIRFGPNTDDH
                     DYNFKLKHAINFLQEGAKVKAYVFFKGRSILFKEQGEILLLRFAQDLEEFGKVEQLPQ
                     LEGKRMIMIVSPKGTKKK"
     gene            781..978
                     /gene="rpmI"
                     /locus_tag="PLW22_02495"
     CDS             781..978
                     /gene="rpmI"
                     /locus_tag="PLW22_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOU73604.1"
                     /translation="MPKTKTHSGSKKRFALTGTGKIKRKHAYKSHILTKKETKQKRNL
                     THVALVDKADEGHVKAMLGLK"
     gene            1090..>1406
                     /gene="rplT"
                     /locus_tag="PLW22_02500"
     CDS             1090..>1406
                     /gene="rplT"
                     /locus_tag="PLW22_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOU73605.1"
                     /translation="MPRSVNAVASRKRRKKILKQTKGNFLARRNVWTVAKNTLEKGLT
                     YAYRDRKKKKIEFRALWIQRINAAVREYGMTYSEFMGKINQKGIAINRKVLADLAMNH
                     PEA"
     assembly_gap    1407..1506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1539..2876
                     /gene="lepB"
                     /locus_tag="PLW22_02505"
     CDS             1539..2876
                     /gene="lepB"
                     /locus_tag="PLW22_02505"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOU73606.1"
                     /translation="MFQKVKDFLKNKYFRFTFAAIIFILWVVWIGNYWLLLGLPIIFD
                     FYITQKVNWTFWKKRGQKKTVLIEWVDAIIFAVVAATLIRMFFIEAYTIPTSSMEKSL
                     LVGDYLFVSKVAYGPKLPNTPISFPFVHHTLPLTKYTKSYIEWPRWPYKRIAGFGQIK
                     RNDVVVFNFPEGDTVCLQDQNSSYYTLARIYGRDYIRQNYDIIYRPVDKRENYIKRCV
                     GIPGDTIQVVHGSLFVNGKEQAKIGKRQYNYQIRTNGSTINPIRFEEMGIARSDINYN
                     AQESVYILPLTEEMLERFKTFSNVTEITRNENTDSTVMWKLIFPHDPRYKWNEDNFGP
                     LWIPKKGATVTLTTDNLPLYQRIIDVYENNDLRVENGTIYINGEPASSYTFKMDYYFM
                     MGDNRHNSADSRFWGFVPEDHVVGKASFIWLSLDKDKRFPANIRFKRMFTTIK"
     gene            2956..3597
                     /locus_tag="PLW22_02510"
     CDS             2956..3597
                     /locus_tag="PLW22_02510"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOU73607.1"
                     /translation="MSSKTILLSTAYLPPVHYMSALLSADKIIIEAHETYLKQTYRNR
                     CVILSANGPLPLVIPVIKPKGNHTGITEVIIDYSTGWNRNHWRAIESAYRTSAYFEFI
                     ADVLKPFYSNPFNTLWEFNNELLKAILDFLELPVNINFTSTFHKVEPEGVLDLRYAIS
                     PKAKMPETSLHATLPYFQVFEHRFGFTPNLSVIDLLFNEGLMARDILRIKDKG"
     gene            3853..6648
                     /gene="uvrA"
                     /locus_tag="PLW22_02515"
     CDS             3853..6648
                     /gene="uvrA"
                     /locus_tag="PLW22_02515"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU73608.1"
                     /translation="MQKHEKVISVKGARVHNLKNINVDIPRNKLVVITGVSGSGKSSL
                     AFDTIYAEGQRRYVESLSAYARQFLGRMSKPEVDLISGIPPAIAIEQKVNTRNPRSTV
                     GTSTEIYDYLKLLFARIGKTYSPISGHEVKRHSVTDVVNFISAHQQGTRMTLLAPIAV
                     NGNIKLKLEELNREGFTRIDINGKVIRIDDAQNRELVDLAAANTRLIIDRFAVSVDED
                     FISRIADSVQTAYAEGNGFCILSIETDEGIREEQFSNLYELDGIKFEQPTEHLFNFNN
                     PLGACPKCEGYGKVIGIDEDLVVPNKSLSIYEDAIACWRGESMGWYKDRVIEYASAYN
                     LPIHTPYHKLTEEQKALLWNGTRHFKGIYDFFAELEEKRYKIQYRVLLSRYMGKTTCP
                     VCKGGRLRPEASYVKVAGYTIQELVNIQIEELYEFFMNIELDEHDVTVAKRLLTEIRN
                     RLGYMVNVGLGYLTLNRLSATLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHPRD
                     THLLIDVLKNLRDLGNTVLVVEHDEDIMRNADYIIDIGPHAGLLGGEVVFAGEPSLIN
                     SASTSLTAKYLNGTEKIEVPFKRRKWNSYIEVKGARENNLKNIDVKFPLNTLTVVTGV
                     SGSGKSSLVKGILYPALLKEITGSGERAGQHDCIAGDIRLISGVEMVDQNPIGKSSRS
                     NPVTYLKAWDEIRKLLSEQPYSKSNGYGPSHFSFNIDGGRCEECQGEGVIHVEMQFMA
                     DLTLTCESCHGKRFKDDILEVRYRNANVYDLLEMTVNQAIEFFEKGKGSLEKKIVEKL
                     KPLQDVGLGYIKLGQSSSTLSGGESQRVKLASFLLKENTDKPILFIFDEPTTGLHFND
                     IKKLLSAFDALIAKGHSIVLVEHNLEVIKCADWVIDLGPEGGDNGGHLIFSGTPEQLA
                     LMDNSYTGKFLSVKLNN"
     gene            complement(6707..7150)
                     /locus_tag="PLW22_02520"
     CDS             complement(6707..7150)
                     /locus_tag="PLW22_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73609.1"
                     /translation="MRSSIYILVSAVVLFLALPSSSNAQCKNFAKKICRLELSPYVHD
                     GNYNAAILTEGEDAELFKTFYAGQQYRISVCGSETLNKIEFMVMDVDRKVLYTNAKNN
                     FATSWDFKVENTQQLIILVKVPVTSGKNEELKSGCVAIMFGLKTD"
     gene            complement(7161..8078)
                     /locus_tag="PLW22_02525"
     CDS             complement(7161..8078)
                     /locus_tag="PLW22_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73610.1"
                     /translation="MHKGSKRILLLSAMAAMAVLTISCKCNRESEQMLQVSEEQGAAD
                     SALVSSIESVKQIFYSLPSPLETAMILKRSGATYNEELLNPIENASKYNTTKSMALNL
                     GIYSTDLSYASLFDQTQATIKYMTVSKKMAEGLGILNAIDNSVIEKLEENINNRDVIL
                     ETISETFLNTNSILEEDDRVAVGSIILVGGWIEGLYIATSLVEDINKVDNEMVDRIID
                     QKLSLQTVLKLLEQSNSNADVRELYSDMLELEKVYNDVKITVSDVKVIQDGKSKVATL
                     KSKNVTTVSPEAFKKLKTKVIELRNKYTA"
     gene            8235..8741
                     /locus_tag="PLW22_02530"
     CDS             8235..8741
                     /locus_tag="PLW22_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73611.1"
                     /translation="MQPNKAEQFQKTIEASSDEAIIGLLTELRETGEDYMLPTILSLL
                     FSTRSEKLKNEVVNFLVDLKNKSSIDTIVEAIRQNKRSKDIHLLVSVCWQSRLDFTPY
                     VDTFIDIMEHGNYQACLEAFTVIENMTENLSGEQLNDLKAKIKSVKGDNAETKPLIEE
                     LVKLLDEL"
     gene            complement(8774..9751)
                     /locus_tag="PLW22_02535"
     CDS             complement(8774..9751)
                     /locus_tag="PLW22_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOU73612.1"
                     /translation="MAKLETSYLGLSLRSPLIVSSSGLNSSVDRIKKYQEFGAGAIVL
                     KSLFEEQILHEVNHLEKYSDYTEATDYLKHYVSANNVQEYINLIKNAKQSVDIPVIAS
                     INCLSDNQWLSFASRIQEAGADALEVNIYYLPLDPKRKAEEYEQVYNSIAEKLQKVIT
                     IPVAYKLGSHFTNPLNVVEHLRYRGAKGVVLFNRFFEPDIDIHAMKVVPADIFSNPSD
                     LRYTLRWVAIASHLIPEIDISASTGVHDGEALVKMLLAGAKSVQVCSAIYRNGPKHIE
                     AMLNTLTEWMNQKGFKTISDFRGKLNVGSIADPTQYERSQFMKYFSNYE"
     gene            complement(9796..10467)
                     /locus_tag="PLW22_02540"
     CDS             complement(9796..10467)
                     /locus_tag="PLW22_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOU73613.1"
                     /translation="MSIPEQLEKIKNELPQHVKLIAVSKTHPVSVIMEAYNAGHKFFG
                     ENRVQELISKYDEMPKDIEWHLIGHLQTNKVKYIAPFVSLIHSVDSLKLLSVIDKEAQ
                     KCNRVIDCLLQVYIASEETKFGLSADELHELLQNQEFEQLQNVRVCGLMGMATFTDNM
                     EQVRMEFRFLKKLFNEIKQTYFQNQPWFNELSMGMSSDYRIAIEEGSTMVRIGSNIFG
                     HRSYT"
     gene            complement(10610..11851)
                     /locus_tag="PLW22_02545"
     CDS             complement(10610..11851)
                     /locus_tag="PLW22_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOU73614.1"
                     /translation="MVKIKAFKGIRPPREIAKEVAARPYDVLNSQEAKAEAGEKSLLH
                     ITKPEIDFDPIIDEHTQQAYDKAVENFRKWKERGWLVQDPKDMYYVYAQTMDGRTQYG
                     LVAACHFEDYMEGRIKKHELTRPDKEEDRMIHIRIQNANIEPVFFAYPAHKEMDEIVT
                     NVVTSKKPEYDFVADDGFGHHFWVIDDEATIKRITEIFASIPALYVADGHHRTAAAAR
                     VGLEKKNANPNHRGDEEYNYFLAVIFPDNQLKIIDYNRVVKDLNGLTPEQFLQKLNEG
                     FVVEEKGSEIYKPQQLHNFSMYLEGKWYSLTAKPGTYNDNDPIGVLDVTILSNLVLDP
                     ILDIKDLRTSKRIDFVGGIRGLGELKKRVDSGEMKVAFALYPVSMKQLIDIADTGNIM
                     PPKTTWFEPKLRSGLVIHALE"
     gene            complement(11883..12230)
                     /locus_tag="PLW22_02550"
     CDS             complement(11883..12230)
                     /locus_tag="PLW22_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU73615.1"
                     /translation="MDHKELDAWKVAVDLSVEIYSLTQSFPKEEIFGLVQQIRRSVIS
                     IPSNIAEGCARGSSKETIHFLYVSLGSLAELETQIIIASKLGFVNGIDELLIKLNKLK
                     QVILGLIRYLKTV"
     gene            complement(12280..13203)
                     /locus_tag="PLW22_02555"
     CDS             complement(12280..13203)
                     /locus_tag="PLW22_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOU73616.1"
                     /translation="MTKVLVATEKPFAKVAVEGIRKIVEDAGFELVLLEKYTDPNDLV
                     KAVADVDALIVRSDKVTKEVVDAGKNLKIVVRAGAGYDNLDLPACTAKGVVAMNTPGQ
                     NSNAVAELAIGMMIYMARNLFQPGTGTEIAGKKLGIHAYGNVGKLVAKYGKGFGMEVY
                     AFDPFVDKAVMEKDGIKVVDSIEMLYSSCDYVSLHIPANDKTKKSIGYDLLSKMPKGA
                     TLVNTARKEVIDEDGLKKVLAERTDLKYATDIMADCHAELVEKFPGRVFATPKKMGAE
                     TAEANINAGLAAARQIVAFIKNGDKTFQVNK"
     gene            complement(13622..14692)
                     /gene="serC"
                     /locus_tag="PLW22_02560"
     CDS             complement(13622..14692)
                     /gene="serC"
                     /locus_tag="PLW22_02560"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOU73617.1"
                     /translation="MKKHNFNAGPCILPEVALENTAKAVLDLDGIGMSILTISHRSKE
                     FETIMEETKALWRELLHIPENYHILFLGGGASLQFCMVPYNLLQSKAAYLDTGVWANK
                     ALKEAKLFGEVVTVASSSDKNYTYIPKGFTIPTDVDYFHITTNNTIYGTEIKHDMDSP
                     VTLVADMSSDIMSRPVDVKKYGLIYGGAQKNVGPAGVTFVIIREDILGKVERKIPTML
                     DYRTHIKDGSMFNTPPCGAIYTMRETLKWLKGIGGVEKINELNKKKAQLLYDAIDNSK
                     MFVGTAEKDSRSIMNICFVMKPEYKDKEDAFMDFAKKAGMVGIKGHRSVGGFRASTYN
                     ALPIESVQALVDCMKEFEKQNA"
     gene            complement(14824..15537)
                     /locus_tag="PLW22_02565"
     CDS             complement(14824..15537)
                     /locus_tag="PLW22_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOU73618.1"
                     /translation="MPRIEVAFSPTIFQHYDKEGKIVVVVDILRATSVICTMFHNGVK
                     EIIPVRSIEEAHEYKRNGYLVVAERNGQKLDFADFGNSPFYFTPETVNGKTIVYSTTN
                     GTNAITLGRDAQEVVIASFLNISAVESYLNARSSDILIMCAGWKGKFCLEDAIFAGAL
                     AQRLLTTGRFETICDSTNAAIDLWNVAKVDLTSYVEKVAQRHRLKKLGLDDVIGYCFT
                     SNVTDTIPVLKEDRLVPLI"
     gene            complement(15584..16693)
                     /gene="gcvT"
                     /locus_tag="PLW22_02570"
     CDS             complement(15584..16693)
                     /gene="gcvT"
                     /locus_tag="PLW22_02570"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOU73619.1"
                     /translation="MKNTPFTKYHIELGARMAPFAGYNMPIEYSGINDEHITVREKVG
                     VFDVSHMGEIWVKGPKALDFLQTVTSNDVSALFDGKVQYSCFPNDKGGIVDDLLVYRV
                     NAETYLLVVNAANIEKDWNWLCEQGKKFGLTPGKELYNASDEFAQLAVQGPLALKAMQ
                     KLTTENVVDMEYYTFKKIPFAGIKEVIFSTTGYTGAGGCEIYCANEDADKLWKAVFEA
                     GAEYGIKPIGLGARDTLRLEAGFCLYGNDINDSTSAIEAGLGWITKFVPGKNFISRDV
                     WEKHKNEGTDRRLKGFVMIDRGIPRQHYLIYDVQGNEIGEVTSGTMSPMSKQGVGMGY
                     IKKGHWNDGNEIYIGIRDKKLKAKIVKPPFHLAKV"
     gene            16854..17216
                     /locus_tag="PLW22_02575"
     CDS             16854..17216
                     /locus_tag="PLW22_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOU73620.1"
                     /translation="MATDQATHHVNLDFLKEISEGSTDLMRDLINLFITQVPTFSEQM
                     EIYLKNQDYCTLAKLAHKIKNSVAMMGIDELTSDMKKLENICTSKPNDDTVKVYVERF
                     KKISGEASVELQHILESL"
     gene            17242..17574
                     /locus_tag="PLW22_02580"
     CDS             17242..17574
                     /locus_tag="PLW22_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU73621.1"
                     /translation="MIDVEKINDVMVISFSENSKLNVTVTQKIKTEVIKVITPNAKVA
                     LNLSGVSYIDSTGFGMLLSILRHCKNSNATLKLCNISPEVMELIKLLQLQTVFDIREG
                     VDDCIKSF"
     gene            17686..20268
                     /locus_tag="PLW22_02585"
     CDS             17686..20268
                     /locus_tag="PLW22_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU73622.1"
                     /translation="MKRAITLLLSFTIGILFSHAQVITSPEEHFGFTPGADRMLFKYN
                     QVVEYLRKVETQSPMVKLTPIGQTAMGKNLYAVFVSSKENIANLDRLKEINKQLAINY
                     SLNNAEREQMVAQGKVFVMATLSMHSTEVGPTQALPTIIYRLISGNTFSNALNNVVYM
                     AVVCHNPDGMDMVVDYYNQFKGSKYEGGPMPFLYQKYVGHDNNRDFITLTQPETKAIS
                     RLTSQEWFPQVMVEKHQMGLTGPRYFVPPYHDPIAQNVDAELYTWSNLFGQNMLNDLT
                     SKGLQGVSQQNLFDNYWPGSTETCLWKNVISMLTEAASAKVATPVYIEPTELTGTSKG
                     LAEYKKGVNMLLPWPGGWWHLSDIVALEVESTISMLQTASKYKERILMFRNDICKKQV
                     SLGKTIAPYHYILPANQQDPSELVSLVNLLKEHGVNVYRLKQNITIQNQNFLPGDIVV
                     PLAQPFRMFIKEVMEPQEFPERRLEEGGKVLEPYDITSWSLPLHKGLTCFTINNNAID
                     NSIIEPIQSSFTLNQKTLPEKGFALLPSTVNQSYKLAVKCLKSGVQVERTNEHTVFDG
                     IELPAGSFCIPLNEKTRQVLNDANFSIMTSDVKPNNTSISKIRRIGLLESQVQSMDAG
                     WTKFILDTYEIPYTVINPSQVAEGNLLSKFDLVIIPNTNATLLKDGKYKRNGQVIPSV
                     YPEIYTKGMGQKGIEALTEFIQNGGTVLAWEQATELFEGTLSLKQGNVSEEFMLPFKN
                     NAKEVQKQGFSCPGSLVRLKLKPVYPFTFGLADELGIFMRDSYLFETQVPSLDMDRRV
                     IGYFEGKSKLLSGYLKGEEIMDGMTAAIWLRKGKGQVILLGFSPIFRASVPSNYKILF
                     NTIL"
     gene            complement(20306..21430)
                     /locus_tag="PLW22_02590"
     CDS             complement(20306..21430)
                     /locus_tag="PLW22_02590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU73623.1"
                     /translation="MSIQIVKVENKTDLRKFIHLPAIIHKGHKNWVPPIYSDEWDFYN
                     PRKNRAMSYSDTVLYLALNNGRPVGRIMGIINRRYNQIHNEKTARFFNLETFQDYDVA
                     QALLGEVEKWAKEKGMDRLIGPMGFSDKDPQGLLVEGFHEPIVLASNCNYPYLVEFVT
                     RYGFEKDIDLVVYKVPVPHELPDFYLRILQRVKSRANGFRMLSFSKRSDIKPFIKPVL
                     SLVNETFVDIYGFNPMDSDEMEELAAKYLSFIDPRFLKVIVNTHNQPLAFILGIPDIC
                     PGIQRSKGYLFPLGFIHILRAMRTSKTLSLLLGAIHPQYRNLGFDTWMGAEMLHEAQK
                     AGMTIIDSHLELETNRKMRAEMEKMGGVVYKRYRIFQKKL"
     gene            complement(21540..22952)
                     /gene="nhaD"
                     /locus_tag="PLW22_02595"
     CDS             complement(21540..22952)
                     /gene="nhaD"
                     /locus_tag="PLW22_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HOU73624.1"
                     /translation="MFLLMVVLFVLGYAAIALEHNIKVNKTASALFLGVALWVLLMLD
                     AGGILGTDIGGRFGEFINSHPELASLPKWEQYVNYIADNQILGFLGEISEILFFLMGA
                     MTIVEIVDQHGGFKIITNRIKTRNKVKLLWIISFVTFFLSAVLDNLTTSIVMVALLRK
                     LIDGKHDRWLYAGMVILAANAGGAWSPIGDVTTIMLWIKGNVTTAGIIPKLFFASLVS
                     LIVPLVALSFTLKGDFAQSLDSSSEKVHSVTPATRRERHLIFILGVGALLFTPVFKVL
                     THLPPFMGMLLGLSVLWIFTEVMYHRKHNLDEREKRTVARVIKNVDVPTILFFLGILM
                     AVDALQTAGHLTLLSQWLDRSFTSPYPPNILIGLLSSVVDNVPLVAAAMGMHPAVSPD
                     LVNTASAISSAYVADGDFWNLLAYCAGTGGSILIIGSAAGVAVMGLEKIDFMWYLKHI
                     SLLALLGYFAGILTYFLMFG"
     gene            23077..24405
                     /locus_tag="PLW22_02600"
     CDS             23077..24405
                     /locus_tag="PLW22_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOU73625.1"
                     /translation="MKALTNTARNFFLPLLLIFTINCYSQGSEDRMQWFSDAKFGIFI
                     HWGIYSLGNTSESWAFYNKQVSHADYMAQINLFSAERYDPAQWARLVMQSGARYVVIT
                     AKHHDGVALWDTKLSNLSMATQSPAARDVLSPFIKALREQGLKVGIYFSLIDWTHPDY
                     HGFTRDSSRYNVADDPLRWQQYLNFCHGQIEEIMQLYSPDLIWFDGDWEHSAKEWQAE
                     KIREIIHRHNPNTIINGRLQGYGDYDTPEQNFPVTRPNLKYWELCMTMNSSWGYRVND
                     TIYKTPFEIVSILTDAISMGGNLLLDITPKADGSIPEEQVKTLSTIGNWIGKHSDAIY
                     GTLPGLPMGHFYGATTLSKDSTSLFLFLPAKTYGQILLKGINSPIKKATILGTNHELL
                     HKVVGKISWSPVPGLIFIDVPKELTDALMTVVRIDFDEPIKLYRGKGGLN"
     gene            complement(24458..26959)
                     /locus_tag="PLW22_02605"
     CDS             complement(24458..26959)
                     /locus_tag="PLW22_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU73626.1"
                     /translation="MMKKVLLLWAIAVVTVTSMAQDRMQGRSGQNFDMSNFNGVITGK
                     VIDSLDRNGVEFANIALYKQKDSSLVTGTLTDASGNFMMEKLMPGRYYVDVKFIGYQS
                     RRISGIMVSPRSPKVDLGTFSLLAASENIEAVVVTGEKKMLLHNLDKKVFNVDKDIAV
                     EGGTAADVLQNIPSVEVDTDGNVSLRGSSNVNILIDGRPSMLNSMEELPAQMIDRVEV
                     ITNPSAKYDPDGITGIINIVLKKRKEPGYNGMVSLNAGTGNKYNATVNMNWRQDKVNV
                     FANYSFRRGQMDRFSIGDRVTLFNSGADSSFLSQNSEGQSNMFFHNIRGGADYFIDSR
                     TTLSFTGNLNFRSFDMDNNVLSNSYSNFNSNTLQNTRISLNSNDGLGHEYSLNFKRTY
                     DTPGKEWTADAFFSRNTNNNLNNINQQEIFNTVPTTALERAETDGWMNTFTFQTDYVT
                     PFGNGGRIETGIKAQIRRSDADYVYKIFNSLGSTWDFDANRSNHFVYNEEIYSAYGIY
                     SNTFANGKFSYQLGLRVEDQHSKSDQRTTNEVADVNRLNLFPSAHIRWEPNPINSLQL
                     SYSRRVNRPSVMVLNPFLNTSDNFNWSKGNPYLEPEFTSSIDLSHNLNFPRTKITTSV
                     YYRDTRNGFSRRMIVIDTVATQPTLTTFINLSHYENIGAEAVVTQNIAKWWRINASYS
                     YYYSKLFGDVVSGANEGSSWNAKLASFFTIGKNVDIQLTGNYRAPSITVGGSGRGFYM
                     AGGAQGETKEMYWVDLGARINVLNKKGTITVRVSDIFNTQKMKYSSWDTNFYSYNESW
                     RDSRVVFVGFSYRINNYKMRPEKKVDSDDSFDMVE"
     gene            complement(27121..27609)
                     /gene="nuoE"
                     /locus_tag="PLW22_02610"
     CDS             complement(27121..27609)
                     /gene="nuoE"
                     /locus_tag="PLW22_02610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOU73627.1"
                     /translation="MSHIKLELKPEQVAKIKEICKSFNNDPGELINVLHKTQETFGYL
                     PAEVQEVVAAELNISVAKVYGVVTFYSFFTMIPKGKHPISICTGTACYVRGAEKVLDE
                     FKRVLNVKVGETTPDGKFSLNCLRCVGACGLAPVVLIGEKVYGRVSPDGVKDIIKEYE
                     DQ"
     gene            complement(27623..29398)
                     /locus_tag="PLW22_02615"
     CDS             complement(27623..29398)
                     /locus_tag="PLW22_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOU73628.1"
                     /translation="METVKLTIDNKQVEVPKGTTIYKAARKLGIDIPVLCYMELHDLG
                     VENKPAGCRICVVEVEGRRNLAPSCATECAEGMVVKTHTTRVINARRTVMELILSDHP
                     KDCLTCPKSGRCDLQDMAIKLGIREIPGQEYAEMSTYKKDFSPSIIRDVDKCIMCRRC
                     ETMCNEVQTVGALSAVNRGFMAVVAPAFEQDLEKSPCTYCGQCVAVCPTGALTEVDHT
                     TKVIRAIADPTKTVLVQTAPAVRAALGEEFGMKPGTLVTGKMVAALRQIGFDYVFDTD
                     FAADLTIMEEGTELLDRLSRFLNGDKNVKLPILTSCCPGWVNFFEHNFTDMLDVPSSA
                     KSPQQMFGAIAKSYFAEKLGKKREELVVVSVMPCLAKKYECQRDEFKVDGNQDVDFSI
                     STRELAHLIKEFNINFEELPDDDFDRPLGESTGAGVIFGATGGVIEAAVRTAYEAYTG
                     KPLPKIDFTELRGMEGIRSATIDFNGLPINIGIAHGLGNARKLLEDVRSGKANYHAIE
                     VMACPGGCIGGGGQPLHHGNSAIIKARMEAIYREDAGKPIRKSHENPYIVKLYEEFLG
                     KPMSEKAHHLLHTHYFSKKKDIYIK"
     gene            complement(29413..31206)
                     /locus_tag="PLW22_02620"
     CDS             complement(29413..31206)
                     /locus_tag="PLW22_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOU73629.1"
                     /translation="MSKYKMHLLICGGTGCKASSSDAIAEKLKLELAANNLQDEVQVI
                     MTGCFGFCEKGPIVKVLPDNTFYTQVKPEDATEIVKEHVVKGRKVERLLYVDPTKKAA
                     IEDSKHMGFYRKQMRIALRNCGFINPENIDEYIAREGYQALGKVLTEYTPQQTIDIIK
                     KSGLRGRGGGGFPTGLKWEIASKNHADQKYVVCNADEGDPGAFMDRSILEGDPHSVLE
                     AMAICGYCIGATKGLIYIRAEYPLAIKRLKVAIDQAREYGLLGSEIFGTSFSFDIELR
                     YGAGAFVCGEETALIHSMEGLRGEPSNKPPFPAQSGYMGKPTNVNNVETFANVPPIIL
                     KGADWYSSIGTEKSKGTKVFALAGKINNVGLIEVPMGITLREVIFEIGGGIKNGKKFK
                     AVQTGGPSGGCLTEKHLDTPIDFDNLIAAGSMMGSGGMIVMDEDDCMVSVAKFYLDFT
                     VEESCGKCSPCRIGNKRLYELLERITKGEGTMEDLERLRNLSQVIKDTSLCGLGQTSP
                     NPVLSTLDNFYDEYVAHVVDKKCPSGQCKSLMQYVIDPEACVGCTACARNCPVNAISG
                     ERKKPHEINQSICIKCGACMEKCKFDAISIK"
     gene            complement(31227..31625)
                     /locus_tag="PLW22_02625"
     CDS             complement(31227..31625)
                     /locus_tag="PLW22_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOU73630.1"
                     /translation="MTKVKSLADLKKMKETLQAKIDLREKGEAAENLVQIKVAMATCG
                     IASGAKNVMDFLLEELEKRGINAVVTQTGCMGYCYAEPTIEVKLPNADPVVFGYVDTK
                     KADEIIEKYIKNGELVDGIIPVNYESIDNK"
     gene            complement(31733..32287)
                     /locus_tag="PLW22_02630"
     CDS             complement(31733..32287)
                     /locus_tag="PLW22_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73631.1"
                     /translation="MKDLSLHILDIVQNSVVAGAKVIQIILDINTANDYIELTVKDNG
                     KGMEQEFLKRVVDPFTTTRTTRKVGLGIPLLKQNAELTGGAFSIDSEPGVGTALKARF
                     VKSHIDCLPLGDLGGTIALLLCSYPDIEFKVSVVVDTTEFAVSTSELRQALDGVPLNE
                     PSVYPLVKEYVYSNLDELQITNLS"
     gene            complement(32332..33072)
                     /locus_tag="PLW22_02635"
     CDS             complement(32332..33072)
                     /locus_tag="PLW22_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU73632.1"
                     /translation="MIVKADLHIHSVISPCADLEMSPVNIVMRAAEMGLKLIAVTDHN
                     STLHGPLIRTLAEQHGIYCLYGAEVNTREEIHCLCLVDTEDQRQLLQAFIEQNITRIP
                     NNPDIFGYQVVVNEHDEIVQEIDYLLHAALAKSIDEIAEFVHGIGGVFIPAHVDRPKY
                     SVTSQLGFVPPSLSYDALEISRNTTVDSFKRNNPIKPNTQFIRNSDSHFLSQIGTSYT
                     EYNLENLSFKCFKDSILNTGSSTVLPVI"
     gene            complement(33069..33407)
                     /locus_tag="PLW22_02640"
     CDS             complement(33069..33407)
                     /locus_tag="PLW22_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOU73633.1"
                     /translation="MTVKDIVDALNLKVYSGEAGLNKEVVGGYTSDLLSDVMGNAPAG
                     SVWITLQTHVNVMAVATLKDISAVILVKGLEPEANTMAQSNNEAIPVLGTNLQAFEIS
                     GQLYNLINKK"
     gene            complement(33420..34787)
                     /locus_tag="PLW22_02645"
     CDS             complement(33420..34787)
                     /locus_tag="PLW22_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU73634.1"
                     /translation="MADLEFYHALKILPDVCIGCSHCMRACPTEALRVVNGKAKLINN
                     RCIDCGECYRVCPTHAITVQQDDFSKIFSYKHRIALIPSVLLGQFSKEISHDDVAQAL
                     LSLGFTSIVEVEDSVEYLIGKLNSYIASTEVKPVISSFCPAIVRLIQVKFPSLVENIA
                     LIRPPLDLTATYIKTSLLEQGVPEKEIGIFYITPCAAKIVAVRHPEGEEKSEIDGIIN
                     MDFIYNRILREIKGKLPTGNEPKIISDLSPRSYSWALTNGEASCIDGRCLAIDGIWNV
                     IEFLEKVENDEIQDVDYLELRACDESCAGGILAPGNRFLAAELIRKNASKVSVWSINK
                     TNDKIVKYDACISGRTALSPLSPRSMLKLDEDMAKAMLKMQRVNQLMCFLPGIDCGVC
                     GAPSCIALAEDVAQGSASVSQCVFLQKIMEQNKLDPEHSFRIMKRIWGENRFRKDCDK
                     PIRNK"
     gene            complement(34800..35216)
                     /locus_tag="PLW22_02650"
     CDS             complement(34800..35216)
                     /locus_tag="PLW22_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73635.1"
                     /translation="MHFEYRVQGGDFVRAGQASSQVKKILKQLNVSPNIIKRVVVALY
                     EGEVNIVAHAFQGKIYVDIETDKIRVVLDDVGPGIPDIDLAMKPGYSTASPIVREMGF
                     GAGMGLPNIKENTDKMKIASVVGVGTRLEFEVNLKE"
     gene            complement(35223..35567)
                     /locus_tag="PLW22_02655"
     CDS             complement(35223..35567)
                     /locus_tag="PLW22_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73636.1"
                     /translation="MKIGELAQLVDGNVVCGHAMLDREIEYAFASDLMSDVLTVRADK
                     LLLITGLANMQAVRTAEMADIPAIVFVRNKTISEDMIQLAEDNGIVLIESPFTMFRAS
                     GILYSKGVKPVY"
BASE COUNT        10497 a         8040 c         7322 g        10120 t
ORIGIN      
        1 gagttacgcc gaatccctta ccttttaatt gttggcgaaa aggaacagga agccaacctt
       61 gttgctgttc gtgttcaagg cgaaggcgat aaaggccaaa tgaaactgga agagtttgta
      121 aaccatataa aggctgaaat tgacaatcag ttaaaggaaa tttagtatta actaaacgtt
      181 aggagggcaa aactatagca gcaccttata tcaacaaaaa gtcggacatc gacacttacc
      241 cgataaacga agaaattcgg gctaagaccg ttcgactggt tggcgaaaac attgagaatc
      301 cgggcattta ccccattagc caggcacgtc aaatggccga ggagatggag ttagacttgg
      361 taatgatttc tgataaggcc gatcccccgg tttgtaaaat actggactat cagaagttcc
      421 tttaccagct caagaagaag caaaaggaaa ttaaggctaa cgctcaaaaa attgtggtta
      481 aggaaattag gtttgggccc aataccgatg atcacgatta caacttcaag ttgaagcatg
      541 ccatcaattt ccttcaggaa ggtgctaagg tgaaagccta cgtgttcttt aagggacgtt
      601 ccattctttt caaggagcaa ggcgaaatac tacttttgcg ttttgcccaa gaccttgagg
      661 agtttggcaa agtggagcag ttgccccagc ttgaggggaa acgaatgatt atgatagtat
      721 ccccaaaagg cacaaagaag aaataacaaa ccattagtaa tcaaataaat acagtgtaga
      781 atgccaaaaa caaagactca ttctggttca aaaaagcgtt ttgctttaac cggaacaggt
      841 aagattaaaa gaaaacacgc ctacaagagt cacattttga ctaaaaagga aaccaagcaa
      901 aagaggaatt taactcacgt tgctttggtt gataaggccg atgaagggca tgttaaggca
      961 atgcttggct tgaagtagtt ttgttttctt ttagtttgtg tgttagttta ctttaatgtt
     1021 taacagaatg aattagccac taagagttca aaaacgttga accgctaatc attcaaaaat
     1081 cgtaaaaaaa tgccacgttc agtaaatgca gtagcatcgc gcaagcgcag aaaaaaaatt
     1141 cttaaacaga cgaagggaaa tttccttgcg cgtcgtaatg tttggacggt tgcaaagaac
     1201 accttggaaa aaggtttgac ctatgcttac cgcgaccgta agaagaaaaa gattgaattc
     1261 cgtgccctat ggattcagcg tattaacgct gcagttcgtg agtatggtat gacctactcc
     1321 gaattcatgg gcaaaatcaa ccagaaaggg attgctatta accgcaaggt tttggctgat
     1381 ttggccatga accaccctga ggctttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnngatt tgcttaaact tgactaaacc acaataccat gtttcaaaaa gtaaaagatt
     1561 tcctaaaaaa caagtatttc cgatttacat ttgcagccat aattttcata ctctgggtag
     1621 tatggattgg caactactgg ctacttctgg gattacctat cattttcgac ttctacatca
     1681 cccaaaaagt aaactggaca ttctggaaga agcgcgggca aaagaagacc gttctgattg
     1741 aatgggttga tgccataata tttgcagttg ttgcggcaac cctaatccga atgtttttta
     1801 ttgaggcata caccatacca acatcatcga tggagaaatc gttgctggtg ggcgactacc
     1861 tatttgtgag taaggttgcc tacgggccta aactacccaa cactccaatt tcattcccat
     1921 ttgtgcatca caccctgcct ttaaccaaat acaccaaatc gtatattgag tggccccgtt
     1981 ggccttataa acgtattgct ggttttggtc agataaaacg caacgatgtg gttgtattca
     2041 acttccccga aggcgatacg gtctgcctgc aggatcagaa ttcaagttac tacaccctgg
     2101 caagaattta tggacgcgac tacatccgtc aaaattatga tattatttac cgccctgttg
     2161 ataaacgcga gaactatatc aagcgttgcg ttggcattcc cggcgacacc attcaggttg
     2221 tccatggtag cctttttgtt aatggcaaag agcaggcaaa gattggtaaa agacagtata
     2281 actaccagat aagaactaat ggctctacca ttaacccaat acggtttgag gaaatgggta
     2341 ttgcccgttc cgatatcaac tacaatgcgc aggaatcggt ttacatcctt ccgttaaccg
     2401 aggaaatgct ggaacgtttt aaaaccttta gcaatgtaac tgaaataacc cgaaacgaga
     2461 atactgactc cacagttatg tggaagttaa ttttccccca cgatccccgc tataaatgga
     2521 acgaggataa ctttgggccc ctatggatac ccaaaaaagg tgcaaccgtt accctcacca
     2581 ccgataacct gccactttac caacgcatta ttgatgttta cgagaacaac gacctgaggg
     2641 ttgaaaatgg cactatttat attaatggtg aaccagcaag ctcttataca tttaagatgg
     2701 attactattt catgatgggt gataaccgac ataactcggc cgacagccgt ttttggggat
     2761 ttgtacccga agaccatgtg gtaggaaaag cttcgtttat ttggctatcg ctcgataagg
     2821 ataaacgttt tcctgccaat atacgcttta agcgtatgtt tacaaccatt aaataggatt
     2881 aacaatggct gcataaaaac ctgttggagg ggaatggccc ccgcaacagg ttttttagtt
     2941 caatgaaagc atacaatgtc cagtaaaacc atactactta gcacagcgta tttaccgccg
     3001 gttcattaca tgtccgcctt acttagtgcc gataaaatta ttattgaggc acatgaaact
     3061 taccttaaac aaacctaccg taaccgatgc gtaatactca gcgcaaacgg cccattaccc
     3121 cttgtaatcc cagtaataaa accaaagggc aaccataccg gtataaccga agttattatt
     3181 gattactcta ccggttggaa caggaatcat tggcgtgcca tcgaatcggc ttaccgaact
     3241 tcggcatatt tcgaatttat tgccgatgta ctaaagccct tttacagcaa ccctttcaat
     3301 accctgtggg agttcaacaa tgaactccta aaggcaattt tggacttcct tgagctaccc
     3361 gttaatatca attttacaag tacctttcat aaagttgaac ccgaaggagt attggattta
     3421 cgttacgcaa ttagcccaaa ggctaaaatg cctgaaacca gcttgcacgc taccctacca
     3481 tactttcagg tttttgaaca tcgctttggg tttactccca acctcagcgt tattgacttg
     3541 ctttttaacg aggggctaat ggcaagagat atattaagga taaaggataa aggataaagg
     3601 ttcaaagttc atggttcatg gttcgaggtt gaaggtttga agttaaagtt ttaaggttaa
     3661 aggataaaaa gacacaaatt agatttccat ttagttacac catttttaaa agttatgatg
     3721 aaagggttag tagaaatttt caaaacaagg gggcttggga aggaaagtat aatttcagag
     3781 aatttgaaaa agcatcatta aaaaatggtt ttgaagtaat acctttgtag gttacatttt
     3841 tccatttcat caatgcaaaa gcacgaaaag gtaatatcgg taaaaggcgc aagggttcat
     3901 aatcttaaga atatcaatgt agatattccc cgtaacaaac tggtagtaat tacgggtgta
     3961 tcgggttcgg gaaaatcatc gttagctttc gataccattt atgccgaagg gcaaaggcga
     4021 tacgtagaga gcctatcggc ttacgcccgt caattccttg gcaggatgag caagcctgag
     4081 gttgacctta ttagcggtat tccgccggcc attgccattg agcaaaaagt taacacccga
     4141 aatccccgct ctaccgtagg cacatccact gagatttacg attaccttaa gttacttttc
     4201 gcccgtatcg gaaaaacata ctctcccatt tcgggccacg aggttaagcg tcactcggtt
     4261 accgatgttg taaactttat cagtgcccat cagcagggaa cacgcatgac ccttttagca
     4321 cctattgccg ttaacggcaa tatcaagctg aagcttgagg aactaaaccg cgaagggttt
     4381 acccgaattg atattaacgg taaagtgatt aggattgatg atgctcaaaa ccgtgaactt
     4441 gtcgatttgg ctgctgccaa cacaaggttg ataatagata gatttgccgt ttcggtagat
     4501 gaagatttta ttagccggat agccgattcg gttcaaaccg cttacgcaga aggaaatggt
     4561 ttttgcattc tttctatcga aaccgacgaa ggaatacggg aggagcaatt ctcgaaccta
     4621 tatgaactcg atgggataaa attcgaacag cccaccgagc atcttttcaa cttcaacaac
     4681 ccacttggtg cttgcccaaa atgcgagggt tacggcaagg taattggcat agacgaggat
     4741 ttggtagtcc ccaacaaaag cctttccatt tacgaggatg ctattgcgtg ctggcgaggc
     4801 gagtcaatgg gttggtataa ggacagagta attgagtatg catcggcgta taacctacct
     4861 atccacaccc cataccacaa attaactgag gagcagaaag cattgctttg gaatggcaca
     4921 aggcacttca agggtattta cgatttcttc gcggagcttg aggaaaaacg atacaaaata
     4981 caataccgag ttttactctc acgctacatg ggcaaaacca catgcccggt ttgtaagggt
     5041 ggacgtctta gacctgaagc atcgtatgta aaggtggctg gttacaccat tcaggagtta
     5101 gttaacatcc agattgaaga gctttacgag ttctttatga acatcgagtt ggatgagcac
     5161 gatgtcacag tggcaaaacg tttgttaaca gagattcgaa accgtttagg atacatggtt
     5221 aatgttgggt taggttattt aacccttaac cgtttgtccg ctaccctttc cgggggtgag
     5281 tcgcaacgca ttaaccttgc cacatcgctt ggcagcagtt tagtggggtc gctctatata
     5341 cttgatgagc ctagtattgg tctgcatccc cgcgacaccc atctacttat cgatgttctt
     5401 aaaaatctgc gtgatctggg aaatactgtg ctagtggtag agcacgatga ggacataatg
     5461 cgcaatgccg attacattat cgatattggg ccgcatgcag gcctacttgg aggtgaggtg
     5521 gtttttgccg gagagccaag cctaatcaac tctgcatcca ctagtcttac cgctaaatac
     5581 ctgaacggaa ccgaaaaaat tgaagttccg tttaagcgtc gcaagtggaa tagctatatt
     5641 gaggtaaaag gggcacgcga gaataacctg aagaatattg atgtaaagtt tccgttaaac
     5701 accctcacag tggttacagg ggtgagcggt tcgggcaaat cgagcttggt taagggaata
     5761 ctataccctg ccctactcaa ggaaattact gggagtggcg agcgggcagg ccagcacgac
     5821 tgcatagctg gcgatatccg tcttatttcc ggtgtggaaa tggttgacca aaaccccata
     5881 ggcaaatcat cgcgcagcaa ccctgttacc tacctcaaag cgtgggacga aatccgcaag
     5941 ctgctttcag aacagcccta ttccaaaagt aacggttacg gcccatcgca tttctccttt
     6001 aacattgatg gtggccgttg cgaggagtgc cagggcgaag gggttattca cgtggagatg
     6061 cagttcatgg ccgacctaac cctaacctgc gagagctgcc acggtaaacg ttttaaggac
     6121 gacatccttg aggtaaggta taggaatgcc aatgtgtatg acttactgga aatgactgtt
     6181 aaccaggcca tagagttctt tgagaaaggc aaaggaagcc ttgaaaagaa aatcgttgaa
     6241 aagctaaagc ctctgcagga tgtaggttta ggctacatca aactagggca atcgtcgagc
     6301 acccttagcg gtggcgagag ccaaagggtt aagctagcat cgttcctgct caaagagaat
     6361 accgataaac ctatcctttt catttttgac gagcctacca ctggcctgca ttttaacgat
     6421 attaaaaagc tgttaagtgc ctttgatgcc ctaattgcaa agggacatag cattgttctg
     6481 gtggaacaca accttgaggt tattaagtgc gccgactggg ttattgattt aggcccagag
     6541 ggtggcgata atgggggaca cttaattttt tcgggcacgc cagaacagct tgccttgatg
     6601 gataattcct acacaggtaa gttcctctct gttaagctta acaattaggt atagcaataa
     6661 aaaaaagccg cttaaagcgg cttttattat ttatgtaagt aattgattag tcagttttaa
     6721 gaccaaacat aatagccaca cacccactct tcaactcctc atttttacct gatgtaaccg
     6781 gcaccttaac aaggattatg agctgttgag tattttcaac cttaaaatcc catgaggtgg
     6841 caaaattgtt tttagcattt gtgtataaca ccttacggtc aacatccatt accataaact
     6901 caatcttatt aagagtttca gagccgcaaa cggatatgcg atactgctga ccagcataga
     6961 aggtcttgaa aagttcagca tcttcaccct cggttagtat tgctgcattg tagttaccat
     7021 cgtgaacata gggcgaaagt tcaagcctac atatcttttt ggcaaagttc ttacactgag
     7081 cattggacga cgaagggagt gcaagaaata gaacaactgc cgaaacaagt atataaatcg
     7141 atgatctcat acgaaccatt ttatgctgtg tatttattac gcaattcaat cacttttgtt
     7201 ttaagctttt taaaagcttc agggcttacg gttgtaacat tcttcgattt cagtgtagca
     7261 accttcgatt ttccatcctg tataactttt acatccgata ctgtgatttt tacatcgttg
     7321 tatacctttt caagttcaag catatcggaa tacagctccc taacatcggc attgctattg
     7381 ctttgctcca gaagtttaag aacggtttgt agcgataact tctgatcgat aatgcgatcg
     7441 accatttcgt tatcaacctt attgatatcc tcaaccagcg aggtggcaat gtaaagaccc
     7501 tcaatccatc caccaacaag aataatagaa ccaacagcta ccctatcgtc ctcctccagg
     7561 atggagttgg tgttgagaaa tgtttccgat atggtctcta ggataacatc gcggttattg
     7621 atattctcct ccagcttctc aattaccgaa ttgtcaattg catttaaaat tcctaaaccc
     7681 tctgccattt tctttgaaac agtcatatac ttaatggtgg cctgggtttg gtcaaaaagg
     7741 cttgcatagc tcaaatcggt gctataaatg cccaggttaa gcgccatgct cttggtagta
     7801 ttgtattttg atgcattctc aataggatta agcaactcct cattgtatgt tgcccctgag
     7861 cgcttaagta tcattgcagt ttccagcggc gatggcaacg agtaaaaaat ctgtttaacc
     7921 gattcaatac tgctaaccaa agcgctatcg gcagcaccct gctcttcgga tacctggagc
     7981 atctgctccg attccctatt gcatttacac gaaatggtaa gcacggccat agcagccata
     8041 gccgatagga gaagtattct tttcgatcct ttatgcataa tggaaccttt gggttaaaat
     8101 tttcaacaat cgacaagtaa atatactata attttatcaa aatcaatatc cagtctcaat
     8161 tttttaggta aacaaatgat tttaaaacca aagtaaaacc aaaagcaata cctttgtaaa
     8221 aaaatttaaa ccctatgcag cccaacaagg cagaacagtt ccaaaagact attgaagcga
     8281 gttccgacga ggcaattatt ggtttgctta ctgaattgag ggaaaccggt gaggactata
     8341 tgcttccaac aatactcagc ttgcttttca gcacgcgttc ggaaaagttg aagaacgagg
     8401 ttgtaaactt cctggttgac ctaaaaaaca aatcgagtat tgatacaata gttgaggcaa
     8461 taaggcaaaa caagaggtcg aaagatattc acctgttggt ttcggtttgc tggcaaagcc
     8521 gcctggactt tactccttac gtcgatactt ttatcgacat aatggaacac ggcaattacc
     8581 aggcttgcct cgaggctttt accgtgattg aaaacatgac cgagaatttg agcggagagc
     8641 aacttaacga tttgaaagcc aaaatcaaat ccgtcaaagg ggataacgct gaaactaaac
     8701 ccctaattga agagttggtg aaattactgg atgaactata aaaaagaacc gccagttggc
     8761 ggttctttca tttttattcg tagtttgaaa aatacttcat aaactggctt cgctcgtact
     8821 gcgttgggtc ggcaatggag cctacattaa gcttaccccg gaaatcggat atcgttttaa
     8881 agcctttctg gttcatccat tctgtaaggg tattgagcat agcttctata tgcttaggcc
     8941 cgtttcggta aatagcagag caaacctgaa ccgacttggc gcctgcaagt agcatcttta
     9001 ccaaggcctc gccatcgtga acacctgttg aagccgatat gtcaatttca ggaatcaaat
     9061 ggctggctat ggctacccac cttagggtat atcgcaaatc cgatggattg ctgaaaatgt
     9121 cggcgggtac caccttcatt gcatgaatgt cgatatcggg ctcaaagaac cggttaaaaa
     9181 gtacaacccc tttggctccc cggtagcgaa ggtgctcaac cacatttaag ggattggtaa
     9241 agtgcgatcc taacttatat gcaacaggga ttgttataac cttttgcagc ttctctgcaa
     9301 tgctattgta aacctgctca tactcctcag ccttacgctt agggtcgagc ggcaagtagt
     9361 aaatatttac ttcaagcgca tcggctccag cctcctgaat acgtgaggca aacgatagcc
     9421 attggttatc gctcaggcag tttatgctag caattactgg gatatcaacc gattgctttg
     9481 catttttgat taggtttata tactcctgaa cattgttggc cgatacgtag tgcttcaggt
     9541 aatcggttgc ttcggtataa tcggaatact tttccaggtg atttacctca tggaggattt
     9601 gctcctcaaa taacgactta agcactatag caccagcacc aaattcttgg tattttttaa
     9661 tgcgatcgac cgatgagttt aacccagaac tactgacaat taacggtgaa cgtagcgata
     9721 gccccaagta ggatgtttct agtttagcca tggttaaagg tttttgaata cgattgaaat
     9781 ttacaaaaat ttagtttagg tatagcttcg gtgtccaaaa atattacttc ctattcgtac
     9841 catggtgctg ccctcctcaa ttgcaatgcg gtaatcgctg gacataccca tggatagctc
     9901 attaaaccag ggttgattct ggaagtaggt ttgtttaatc tcgttgaaca gcttcttaag
     9961 aaacctaaac tccatccgaa cctgttccat attgtcagta aatgttgcca ttcccataag
    10021 gccgcatacc ctaacatttt gtaactgttc aaactcttgg ttttgaagta gttcatggag
    10081 ttcatcggcc gataggccga actttgtctc ttccgatgca atgtaaacct gtaacaagca
    10141 gtcaataacc ctattgcact tttgcgcttc cttatcaatt acagagagca gctttaagct
    10201 atcgactgaa tgaataagcg acacaaaggg agcaatgtat tttaccttat tggtttgtag
    10261 gtgcccgatt aaatgccact caatatcttt aggcatttca tcatactttg aaataagttc
    10321 ctgcaccctg ttttcgccaa aaaatttatg cccggcatta tacgcctcca taatcacact
    10381 cacggggtga gttttactta cagcaatcaa cttaacatgc tgtggtaatt cattcttgat
    10441 tttttcaagt tgctcaggaa tgctcatgat tacatagtgt ttaatgcaaa aataaacact
    10501 tttggcttag ctccactaca ggttatacca ttttgaaaac gattgttgaa ctatggcgat
    10561 taaaaaagac tcagcaaatg ctgagtcttt taaaaatttc atttttacac tattccaaag
    10621 cgtggattac caacccgctg cggagcttag gctcaaacca ggtggtttta ggaggcatga
    10681 tattgcctgt atcggcaata tcaataagtt gcttcatgga aacagggtaa agtgcaaaag
    10741 ccactttcat ctcaccgcta tccaccctct ttttaagctc acctaaacca cggatgccgc
    10801 caacaaaatc gatacgcttt gaggttctta gatccttgat atcgaggata ggatcaagca
    10861 caaggttcga caggatggta acatccagaa cgccaatggg atcgttatcg ttgtaggtgc
    10921 caggtttagc ggttagggaa taccacttac cttccaggta catgctgaaa ttgtgtagtt
    10981 gctgcggctt gtaaatctct gagccttttt cctcaaccac gaaaccttcg ttaagttttt
    11041 gaaggaactg ttcaggagta agaccattca aatccttaac tacacggtta tagtcaataa
    11101 tcttaagctg attatcgggg aatattacgg caaggaagta attatactcc tcgtcgccac
    11161 ggtggttagg attggcattc tttttctcaa gtcccacacg agctgcagca gcagtacggt
    11221 ggtgaccatc ggcaacgtaa agggcaggga ttgatgcaaa tatttcagtg atacgcttaa
    11281 tggttgcctc atcgtcaatt acccagaaat ggtggccaaa gccatcgtcg gcaacaaaat
    11341 cgtattccgg ttttttgctt gtcactacgt ttgttacaat ctcgtccatc tccttatgag
    11401 cggggtaagc aaagaaaacg ggctcaatgt tggcgttttg gattcggatg tgaatcattc
    11461 tatcctcttc cttgtcggga cgggttagct cgtgcttttt gatacgtccc tccatgtaat
    11521 cctcaaaatg gcaggctgct actaagccgt actgagttct accatccatg gtttgggcat
    11581 acacgtagta catatccttg gggtcctgaa ccaaccagcc acgttccttc catttgcgga
    11641 agttctccac cgccttatcg taagcctgtt gggtgtgctc atcgattatt ggatcaaaat
    11701 cgatttcggg cttagtgata tgtaacaacg atttttcccc tgcttcagct tttgcttcct
    11761 gtgagttaag cacatcgtac ggacgtgcag ccacctcctt tgcaatctcc cttggtggac
    11821 gaataccctt aaatgcttta atttttacca taatttaaac gttaaatgtt aaacgtgaga
    11881 cgttaaacgg ttttcaaata tcgaattaat cctaaaataa cttgctttaa tttgttcaac
    11941 tttattaaaa gctcatcgat tccattaaca aagcccaatt ttgaagcaat tattatttgc
    12001 gtttccaatt cagccaatga gccaagagag acataaagga aatgtatggt ttcctttgaa
    12061 ctacccctag cacatccctc ggcgatatta gatggtatcg agataactga acgtcttatc
    12121 tgttggacca acccaaaaat ttcctctttt ggaaaactct gagttaagga atatatctca
    12181 actgataaat caacggcaac tttccaagca tctaattcct tatggtccat cccttaatta
    12241 attcggataa cgtttaacgt ttaacgccta acgtaccaac tacttattta cctgaaaagt
    12301 tttatcgcca ttcttgatga atgccacaat ttgacgtgca gcagctaagc ctgcgttaat
    12361 gtttgcctcg gcggtttcag cacccatctt cttgggtgtg gcaaataccc tgcccgggaa
    12421 cttctcaacc agctcagcat ggcagtcggc cataatatca gtggcatact ttaaatcggt
    12481 acgctcagca agaacctttt tgaggccatc ctcgtctatt acctccttac gggcagtgtt
    12541 cacaagggtt gcacccttgg gcattttgct cagtaagtcg tagccaattg atttttttgt
    12601 tttatcgttg gccggaatgt gaagcgaaac gtagtcgcac gaggagtaaa gcatttcgat
    12661 gctatcaacc accttaatac cgtctttttc cattactgcc ttatcaacaa aaggatcaaa
    12721 ggcataaacc tccatgccaa atccctttcc gtacttggca accagtttgc ccacgttgcc
    12781 ataggcgtga atacctaatt ttttaccagc aatctctgtg cctgttccgg gctggaaaag
    12841 attacgagcc atatagatca tcatgccaat agctagctca gcaactgcat ttgagttttg
    12901 accaggagta ttcattgcca caactccctt agcagtgcaa gcaggtaaat cgagattgtc
    12961 gtaaccggca ccggcacgaa ccacaatctt caggttctta cctgcatcaa caacttcctt
    13021 ggtaacctta tcgctgcgta cgataagtgc atcaacatcg gctacagcct taaccaaatc
    13081 gttaggatcg gtgtactttt caagcaaaac caattcaaac ccggcatcct caacaatttt
    13141 tcgtattcct tccacagcaa cctttgcaaa aggtttctca gtggccacta gtacttttgt
    13201 catagatttt aagttttgag ttactttatt ggttcattgg tcagaaatca gaattaggat
    13261 atcaaactca tgaatctcct taattcttta ggttacttcg gtttcgtgtc gactttataa
    13321 atgaataaag cattttgctt tcttctaatt aataagttat acacagaact atacctatcc
    13381 aacctaaacc catcgataaa aaccgaaata tcctagcatt ttgcgataga gccctataga
    13441 tacctgggta ccttttgctc caaaatccat ttttcacaat tccttccagc aaagtttgat
    13501 gaaggctcat ggaaaatggc ttattaattt gaaaaatact taaagagaga aaatgatgaa
    13561 accttaattc taaaaaaatg ggctgaacag gaatgaaccc attcaaccca tctgaattaa
    13621 attaggcatt ttgcttctca aattccttca tgcagtcaac cagagcctga acactttcaa
    13681 taggcaaagc gttgtaggtt gaagcgcgga agccacccac agagcggtga cctttaatac
    13741 caaccattcc tgccttcttt gcaaagtcca tgaaagcatc ctctttatcc ttatactcag
    13801 gtttcataac aaagcaaatg ttcatgatag aacgcgaatc cttttcggca gttccaacaa
    13861 acattttgct attgtcaata gcatcgtaga gtagttgagc cttcttcttg tttaactcgt
    13921 tgattttttc aacgcctcca attcccttaa gccatttaag ggtttcgcgc atggtgtata
    13981 tagcaccgca aggaggtgtg ttaaacattg aaccatcctt aatatgggtg cggtagtcga
    14041 gcattgtagg aatcttgcgc tcaactttgc ccagaatatc ctcacggata ataacaaagg
    14101 taacgccggc agggccaaca ttcttttgag cgccaccgta aatcaaccca tactttttaa
    14161 catcaaccgg acggctcatg atatcggaac tcatatcggc aaccagggtt accggcgaat
    14221 ccatatcatg cttaatctca gttccgtata tggtgttgtt ggtggtaatg tggaagtaat
    14281 ccacatcggt tggaatggta aacccttttg gaatataggt gtaattctta tcggatgagg
    14341 aagcaacggt aacaacctca ccaaaaagct tggcctcttt cagagccttg ttagcccata
    14401 ccccagtatc caggtaagca gctttgcttt ggagtaggtt gtatggaacc atgcagaact
    14461 gcaagcttgc accgcctccc aggaagagaa tatggtagtt ttctggaatg tgaagcaatt
    14521 cacgccagag tgctttggtt tcctccataa ttgtctcaaa ctcctttgaa cggtgcgaga
    14581 tggtcaggat tgacatgcca ataccatcaa ggtcgagaac tgctttggca gtgttttcaa
    14641 gtgctacttc gggcaggata cagggacctg cattgaaatt atgttttttc atggcgttaa
    14701 atggtttaag ttttcaactg ttattgcaag aataggtcaa atttcaaagt tttttttcaa
    14761 atttcctaaa taaatgttat caaacaggaa ttttgaaccg atttttaaca atcgtttgca
    14821 atgctaaatt aaaggcacca acctatcctc tttgagaact ggaattgtat cggtaacatt
    14881 ggaggtaaag cagtaaccaa taacatcatc caagcctaac tttttaaggc gatgtcgctg
    14941 agcaactttt tcaacgtagc tggttaaatc tactttagcc acattccaaa gatcaatagc
    15001 agcattggtt gaatcgcata tggtctcgaa acggccagtg gttaaaagcc tttgggccaa
    15061 tgctcctgca aaaatggcat cctcaagaca gaacttgcct ttccaaccgg cacacatgat
    15121 aagtatatcg cttgagcggg cgttaaggta actttcaacg gcagagatat ttaaaaacga
    15181 ggctataacc acctcctggg catcacgtcc taaagtaatt gcatttgttc cgttggttgt
    15241 gctatataca atagttttcc cgttaacagt ttctggggta aagtaaaatg gagaatttcc
    15301 aaaatcggca aagtcgagct tttggccatt gcgttcagca accacaagat agccattgcg
    15361 tttatattca tgagcctcct caatgctgcg cacaggaatt atttccttaa ccccattgtg
    15421 aaacatggtg caaattactg aggttgccct gaggatatca accaccacaa ctattttacc
    15481 ttctttatca tagtgctgaa agatggtagg cgaaaatgca acttcaatcc ttggcatggt
    15541 atagtttgat ttaaaaaaag ggggctgtgc ccccttttac ggtttatact tttgctaagt
    15601 gaaagggagg cttaacaatt ttagccttta atttcttatc gcggatacca atataaatct
    15661 cattgccatc gttccagtgg ccttttttga tataccccat acctacaccc tgcttactca
    15721 taggcgacat ggttcctgag gtaacttcgc caatttcgtt accttgtaca tcgtaaatca
    15781 agtagtgctg gcgaggtatt ccgcgatcaa tcatcacaaa gcccttcagg cggcgatcgg
    15841 ttccttcgtt tttatgcttt tcccatacat cgcggctaat gaagttcttg ccggggacaa
    15901 acttggttat ccagcctaag cctgcctcaa tggccgaagt agaatcgtta atatcgtttc
    15961 catacaagca gaaaccggct tccagacgga gcgtatcgcg ggcgcctaat ccaattggct
    16021 taatgccata ctcagcgcct gcctcaaaaa ctgccttcca tagtttatcg gcatcctcgt
    16081 tggcgcagta tatttcgcat ccaccagcgc cggtgtaacc tgtagtagag aatattacct
    16141 ccttaatccc tgcaaatggg attttcttaa aggtgtagta ttccatatca accacattct
    16201 ctgtagttaa cttttgcatg gccttcaagg ctaaaggtcc ctgaacggca agctgcgcga
    16261 attcgtcgga agcattatat aactccttac cgggagttaa accaaacttt ttaccctgct
    16321 cgcaaagcca gttccagtcc ttttcaatat tagcagcatt caccactaaa aggtaggttt
    16381 cagcattaac cctgtaaact aaaaggtcgt caactattcc gcccttatca ttcgggaaac
    16441 agctatactg aactttaccg tcgaataatg ctgaaacatc gttactggta acggtttgaa
    16501 gaaaatcaag cgctttaggg ccttttaccc aaatttcgcc catgtgcgaa acatcaaaaa
    16561 caccaacttt ctcacgaaca gttatgtgct cgtcgttaat accagaatac tcaatgggca
    16621 tattatagcc tgcaaatggt gccatacggg ctccaagctc tatatggtac tttgtaaatg
    16681 gtgtattttt cattggtttt atgtatttat ttacagtacc gcaaatctat gatttttatt
    16741 tgtttcttgt tatttaaacc gatttcgatg taaactttta tgcttatgtt tttcaacata
    16801 tcatattttt ttagtaaatt tgaaagtagt agtattctca atctttttaa aagatggcta
    16861 ccgatcaggc aacccaccat gtaaacctcg attttctgaa agagatttcg gaaggaagta
    16921 ccgatttaat gcgcgatttg attaaccttt tcattaccca ggtacccacc ttctctgagc
    16981 aaatggaaat ctaccttaaa aatcaggatt actgcaccct ggcaaaactg gcccataaaa
    17041 ttaagaactc ggtggccatg atgggaattg atgagcttac atccgatatg aaaaaacttg
    17101 aaaacatttg caccagcaaa cctaacgatg acactgtaaa ggtttacgtg gaaaggttta
    17161 aaaaaatatc aggcgaggct tctgttgaac tacaacatat tcttgaatcg ttataagtat
    17221 aaactttaaa aacttacata tatgattgat gttgaaaaaa ttaacgatgt aatggtgatc
    17281 tcgttcagcg aaaatagcaa gctgaatgta accgttacac aaaagattaa aactgaagtt
    17341 ataaaagtga ttactccaaa tgcaaaggta gcattaaacc taagcggtgt tagctatatc
    17401 gatagcacag gttttggcat gttgctatca atactccgtc attgtaaaaa ttcaaacgcc
    17461 accttaaagc tatgcaacat cagccctgaa gtaatggaac tcatcaagct attacaactt
    17521 caaaccgtat tcgatataag ggaaggcgtt gacgattgta ttaagagttt ttaatcgcaa
    17581 gcataatact tttaaaagcc acgataaaac tcgtggcttt tttacttgta aaccatttga
    17641 aaaccaaata gtcaaaggga agtaaccaac ctaaatcaaa atagtatgaa acgtgcaatc
    17701 acattgcttt tatcctttac tattgggata ttgttttcac atgcacaggt aataacatcg
    17761 ccggaagagc atttcggttt cacacccgga gccgatcgta tgcttttcaa gtacaatcaa
    17821 gttgttgaat acctcagaaa ggttgaaacc caaagtccaa tggttaagct aacaccaatt
    17881 ggtcaaacag ctatgggaaa aaatctttat gcggtatttg taagcagtaa agagaatatc
    17941 gctaaccttg ataggcttaa ggagattaac aaacagttag ccattaacta ttccctaaac
    18001 aatgctgaaa gggaacaaat ggtagctcaa ggcaaggttt ttgtgatggc taccctctcc
    18061 atgcattcca ctgaggttgg gcctacccag gcccttccaa caataattta caggttgatt
    18121 tctggaaata cgttcagcaa tgctcttaac aacgttgttt acatggcagt tgtttgccac
    18181 aaccccgacg ggatggacat ggttgttgat tactataacc aattcaaagg atcaaaatac
    18241 gagggtggcc caatgccatt tctttaccaa aagtatgttg gacacgacaa caaccgcgat
    18301 tttattaccc taacccagcc ggaaacaaaa gccatatcca ggctaaccag ccaggaatgg
    18361 ttccctcaag ttatggttga gaagcaccag atgggactaa cgggtccccg gtactttgta
    18421 ccgccctacc acgacccaat cgcccaaaat gttgatgcag aactttacac ttggtcgaac
    18481 ctttttgggc aaaacatgct aaacgacctt acctccaagg gtttgcaggg cgttagccaa
    18541 caaaatcttt ttgacaacta ctggccaggc tcaactgaaa cctgtttgtg gaaaaatgta
    18601 ataagcatgt taaccgaggc agccagtgcc aaagttgcca cacccgtata tattgaacca
    18661 actgagctaa ccggcacctc gaaagggttg gctgagtata aaaaaggtgt aaacatgctt
    18721 ttaccatggc ctggcggctg gtggcattta tcggacattg ttgcgcttga agttgagtca
    18781 accatctcaa tgctacaaac tgccagcaag tacaaggaac gtattctaat gttccgaaac
    18841 gatatctgca aaaaacaggt aagcctgggc aaaacaatcg ctccctacca ttatatactt
    18901 ccagccaatc agcaggatcc aagcgagctt gtttcgttag taaacttgct caaagagcac
    18961 ggcgtaaatg tttaccggtt aaagcaaaat atcactatac agaatcaaaa tttccttcct
    19021 ggcgatattg ttgttccact tgcacagccc ttccggatgt ttattaaaga ggtgatggaa
    19081 ccccaggagt tccctgaacg gcggttagag gagggcggaa aagtattgga accatacgat
    19141 ataaccagtt ggtcgttacc attgcataag ggtttaacct gttttaccat aaataacaat
    19201 gctattgaca acagcattat tgaacccata caatcaagct tcacgctaaa ccaaaaaacg
    19261 ttacccgaaa aaggctttgc tttgcttcct tcaacagtaa accagtccta caaattggca
    19321 gttaaatgcc taaagagtgg cgttcaggtg gagcgaacta acgaacatac tgtgttcgat
    19381 ggcattgaat taccagcagg aagtttctgc attcccctga atgaaaaaac cagacaagta
    19441 cttaatgatg cgaattttag tataatgact tcagatgtca aacccaacaa taccagcatc
    19501 agcaagatac gtcggatagg tttacttgaa tcgcaggttc aaagcatgga tgcaggttgg
    19561 actaagttta tcctcgacac ttacgaaatt ccatacactg ttataaatcc ttcgcaggtt
    19621 gccgaaggca acttactatc gaaattcgac ctggttatta ttccaaacac caacgcaacc
    19681 ctgctcaagg atggcaaata caagaggaat gggcaagtta ttccgtcggt ttaccccgaa
    19741 atttatacca agggaatggg acagaagggg attgaggcct taaccgaatt cattcaaaat
    19801 ggaggaaccg ttttagcctg ggagcaggca actgaactgt ttgagggaac gttatcactc
    19861 aaacagggta atgtttccga ggagtttatg ttacccttta agaataacgc aaaggaagtt
    19921 caaaagcaag gcttttcctg tcccggctct ttagtcagat taaagttaaa acctgtttac
    19981 ccattcactt ttggacttgc tgacgagttg ggaattttca tgcgcgatag ctacttgttt
    20041 gaaacccaag ttccttcgct cgacatggat cgaagagtaa ttggatactt tgaaggaaag
    20101 agtaaactcc taagcggtta ccttaaaggc gaggagataa tggatggcat gaccgcagca
    20161 atatggctca ggaaagggaa aggacaagta attttgcttg gttttagccc aatattcagg
    20221 gcatcggttc catcaaacta taaaattctt ttcaacacaa tcttgtaaaa tagccaaggg
    20281 gttgcatttt gcaacccctt gttttttata gttttttctg gaaaatccta tagcgtttgt
    20341 aaacaacacc gcccattttt tccatttcgg cacgcatctt acggtttgtt tctagctcaa
    20401 ggtggctatc aataatagtc atacctgctt tctgagcctc gtgtagcatt tcggccccca
    20461 tccaggtatc aaatcctaaa ttcctatact gcggatgaat ggcaccaagc aaaagactta
    20521 gcgtttttga ggttcgcatt gctcttagga tatgaataaa ccctaatgga aaaaggtatc
    20581 ccttactcct ttgaataccg ggacatatat cgggaatacc aagtatgaaa gccaaaggtt
    20641 gattgtgggt attaactata accttcaaaa agcgtggatc gataaatgaa aggtattttg
    20701 cagcaagctc ctccatttca tcggaatcca tggggttaaa accatagata tcaacaaagg
    20761 tttcgttaac cagtgaaaga acgggtttaa tgaatggttt gatatcgctt cttttggaga
    20821 aactaagcat tctaaagccg tttgctcggg atttaacccg ttgaagtatg cgaaggtaaa
    20881 agtcgggtag ttcatgggga acaggaacct tgtatacaac caaatcaatg tccttctcaa
    20941 acccatatcg ggtaacaaat tcaacaaggt aggggtagtt acagttcgat gccagaacaa
    21001 ttggttcatg gaaaccctca actagtaaac cctgagggtc tttatcggaa aaacccattg
    21061 gaccaattaa cctatccata cctttttcct ttgcccactt ctcaacctct cccaaaagtg
    21121 cctgcgcaac atcgtaatcc tgaaatgttt ccagattgaa aaagcgggct gttttttcgt
    21181 tatggatttg gttatagcgc ctgttaatta tccccattat tcgtcctaca ggcctaccat
    21241 tatttaaagc gaggtatagc acggtatcgg aatacgacat ggctctgttt ttcctgggat
    21301 tgtaaaaatc ccactcatca gagtaaatgg gaggtaccca attcttatgc cccttatgga
    21361 ttattgctgg taagtgaatg aattttcgga gatccgtttt gttctctacc ttaactattt
    21421 gaatgctcat ttaagtaatt ttagaataca acccacaatg ttaaaaatta tttgagtgat
    21481 aaacaaaaaa tccgcagtga aatttctcac tacggacttt gcttagtcaa tacatagtat
    21541 tacccaaaca tcaaaaaata tgtaagtatt ccagcaaagt atccaagtaa tgctagtaaa
    21601 ctgatgtgtt ttaaatacca cataaaatcg attttttcaa gtcccattac agccaccccg
    21661 gcggcagaac caataatcag aatgctacct ccagtaccag cacaataagc caaaagattc
    21721 caaaaatcac catcagccac ataggctgaa cttattgcac ttgcagtgtt taccaaatcc
    21781 ggcgaaaccg cagggtgcat gcccatggca gcagctacaa gtggaacatt atctacaacc
    21841 gatgaaagaa gacctataag aatgtttggt ggatagggac ttgtgaatga cctatcgagc
    21901 cattgcgaga gcaaggttaa atgtccggct gtttgtaagg cgtcaacagc cataaggata
    21961 cccaaaaaga acagtatggt aggaacatct acattcttta taacacgagc tactgtgcgt
    22021 ttctcccttt cgtcaaggtt atgcttacgg tgatacatca cctcagtgaa tatccaaaga
    22081 accgataaac ctaaaagcat tcccatgaat ggaggcaaat gggttaacac cttaaatact
    22141 ggtgtgaaca gcaaagcacc aacacccaga ataaagatta agtggcgctc ccgacgggtt
    22201 gcaggagtta ctgaatgaac cttttctgag ctagaatcga ggctctgggc aaaatcgcct
    22261 ttaagtgtaa atgaaagtgc tacaagtggg actattaagc taactaaact agcaaagaaa
    22321 agctttggaa taataccagc cgttgttaca ttacccttta tccaaagcat tatggttgtt
    22381 acatcgccaa taggcgacca agccccacct gcgtttgcag caagtataac cattccagcg
    22441 tacaaccatc tatcgtgctt gccatcaata agcttgcgaa gcaaggccac cataacaatt
    22501 gaggttgtaa gattatccag aactgccgag aggaaaaagg taacaaacga tattatccac
    22561 aagagcttaa ctttattgcg ggtcttaatc ctattggtta ttattttaaa tccaccatgc
    22621 tggtcaacaa tctcaacaat agtcattgct cccattagga agaatagaat ctcagagatc
    22681 tctcccaaaa acccaagtat ctgattatct gcaatataat taacatactg ttcccatttt
    22741 ggcaacgatg ccaattcggg atgactgtta ataaactccc caaaccttcc accaatatca
    22801 gtacctaaaa ttcctccagc atcgagcata agcaaaaccc acaaggcaac accaagaaaa
    22861 agcgccgatg ctgttttgtt taccttgata ttgtgctcaa gtgctatggc tgcatagcct
    22921 agaacaaaaa gaactaccat taacaaaaac atagcattat actttttaaa tttgggtgca
    22981 aatgaataaa agaaatttca tattgaaaaa tatagatact tttatttact tgttaatcga
    23041 ttaacagatt ctaacgtatc acataaagcg tttaaaatga aagctctgac aaatacagca
    23101 agaaactttt tcttaccact ccttttgatt tttacaatta attgctactc ccaggggagc
    23161 gaagatcgta tgcaatggtt ttccgatgca aaatttggaa tcttcataca ctggggcata
    23221 tactcgttgg gaaatacatc tgaatcctgg gctttttaca acaaacaggt atcgcatgcc
    23281 gactatatgg cacaaataaa cctgttcagc gcggagcgct acgatcctgc ccagtgggca
    23341 aggctagtta tgcagtcggg agcaaggtac gttgttatca cagctaagca tcacgatgga
    23401 gttgcattgt gggataccaa actcagcaac ctctccatgg caacgcaatc gccagcagcc
    23461 cgcgatgttc tttcgccatt tattaaagct ttacgggaac aaggattgaa ggttggaatt
    23521 tacttctcat taattgattg gacacacccc gattaccacg gatttacaag agactcgagc
    23581 cgttacaatg tagccgatga ccccctaagg tggcaacaat acctaaattt ttgccacggg
    23641 caaattgaag agattatgca actctactcc cctgatttaa tctggttcga cggcgactgg
    23701 gagcactcgg ccaaggagtg gcaagccgaa aaaattagag aaattattca taggcacaac
    23761 ccaaatacca ttattaatgg ccgattacag ggttatggcg attacgatac gccagagcaa
    23821 aactttccgg taacaaggcc taacctgaaa tactgggaac tttgcatgac catgaatagc
    23881 tcctggggat accgggtaaa cgatacaatc tataaaactc cctttgaaat agttagcatc
    23941 cttaccgatg ctatcagcat gggtggaaac ctgttgctcg atatcacccc aaaagccgat
    24001 ggctcaattc cggaagaaca ggttaaaacc cttagcacca ttggaaactg gattggtaaa
    24061 cattccgatg caatttatgg cacactcccg ggtttgccta tggggcactt ctatggcgct
    24121 actacccttt caaaggattc aacatcgcta tttctattcc tcccggccaa aacttatggc
    24181 caaatcctgc tgaaaggaat taacagccca ataaaaaagg caacaatttt gggaacaaac
    24241 catgaactac ttcataaagt tgttggtaaa atttcatgga gtccggttcc aggactcatc
    24301 ttcattgatg ttcccaagga gctaactgac gccttaatga ccgtggttag gattgatttt
    24361 gatgagccaa taaagcttta ccgtgggaaa ggtggtttaa actaaaagga aagccgctca
    24421 aaacaagcgg ccttcatcca acctaaacca gaataaacta ttcaaccata tcaaaagaat
    24481 catcggaatc gacctttttc tcaggtctca tcttatagtt gttaattctg tatgagaatc
    24541 caacaaacac taccctgctg tcgcgccagc tttcgttata cgagtaaaag tttgtatccc
    24601 atgaactata cttcattttt tgggtattaa aaatatcgct caccctaact gtgatagttc
    24661 cctttttatt tagcacattg atgcgtgcac ctaaatcaac ccagtacatc tccttggttt
    24721 caccctgtgc gccacctgcc atatagaaac ctcggcctga accgccaaca gtaattgagg
    24781 gtgcacggta gtttccggtc aactgaatat caacattctt tccaatggta aaaaacgaag
    24841 ccagcttagc gttccaggaa cttccctcgt tagctccgct aaccacatcg ccaaacagct
    24901 tagagtagta gtagctgtag ctagcattaa ttctccacca tttagcaatg ttttgtgtta
    24961 caactgcctc ggcaccaata ttctcatagt gcgaaaggtt aataaaggtc gtaagtgttg
    25021 gttgtgttgc cacggtatca ataacaatca ttcgcctgga gaagccattt cgggtatcgc
    25081 ggtagtaaac cgatgtggta atctttgtcc ttgggaagtt gaggttatgg cttaaatcaa
    25141 ttgagctggt aaactcaggc tcaaggtagg gatttccctt cgaccagtta aaattatcgg
    25201 aagtatttag aaatgggttt aacaccatca cgcttggtct gtttaccctg cggctgtatg
    25261 atagctgaag gctgttgatg gggtttggtt cccaacggat atgcgcactg ggaaacaggt
    25321 tcaacctatt tacatcggca acctcattgg ttgttctctg gtctgatttt gagtgttggt
    25381 cctcaacccg caaacctaac tggtagctaa actttccgtt agcaaaggtg tttgaataaa
    25441 ttccatatgc ggaataaatc tcctcgttgt aaacaaagtg attggagcgg ttagcgtcaa
    25501 aatcccatgt actgcctaat gaattgaaga ttttatacac gtagtcagca tcgcttcgtc
    25561 ttatttgtgc ttttattcct gtctctatcc ttcctccatt cccaaatggt gtaacatagt
    25621 cggtttgaaa ggtaaaagtg ttcatccacc cgtcggtttc ggcgcgttca agagcagtag
    25681 tgggtacggt attaaaaatc tcctgctggt tgatattatt taggttgttg ttagtattac
    25741 gcgaaaagaa cgcatcggcg gtccactcct taccaggagt atcgtatgtt cgcttgaagt
    25801 tgagcgagta ctcatgccca aggccatcgt tggagtttag tgaaattctt gtattctgta
    25861 gggtgttact attgaagtta gagtagctat tcgataatac attattatcc atgtcgaacg
    25921 aacggaaatt taagtttccg gtgaacgaaa gggttgtcct ggagtcaata aaatagtcgg
    25981 caccgccacg tatgttgtga aaaaacatgt tcgattgtcc ctcggagttt tgcgaaagga
    26041 atgaggagtc ggcaccactg ttaaagaggg ttaccctatc gccaatggaa aacctatcca
    26101 tttgacccct acgaaaacta taattggcaa acacgtttac cttatcctga cgccaattca
    26161 tgtttacggt tgcgttatac ttattgccgg ttccagcatt taagcttacc attccattat
    26221 aaccaggttc cttacgtttt ttaagcacta tgttgataat accggttatt ccatcgggat
    26281 cgtacttggc cgagggattt gtaataacct caaccctatc aatcatttga gcaggtaatt
    26341 cctccatgct gtttaacatg gatggacggc catcaatgag aatattgacg ttagagctgc
    26401 cccttaagct tacattacca tcggtatcga cctcaaccga tgggatattc tgtagtacat
    26461 cggctgcagt tcctccctca accgctatat ccttatcaac attgaaaacc tttttatcaa
    26521 ggttatggag cagcattttc ttttcgccgg taacaacaac cgcttcgata ttctcactgg
    26581 ctgccagtaa tgagaaagtt cccaaatcga ccttagggct acgtggcgaa accataattc
    26641 cgcttatcct tctgctctgg tagcctataa attttacgtc aacatagtac cggccgggca
    26701 tcagcttttc catcataaaa ttacccgatg catcggtaag ggttccggtt acaagtgagg
    26761 aatccttctg tttatagagc gcaatgtttg caaactcaac accatttcta tctagcgagt
    26821 cgataacctt tccggtaatc accccgttaa agttgctcat gtcaaaattc tgtccactac
    26881 ggccctgcat tctatcctga gccatggagg ttactgtaac aactgcaata gcccaaagca
    26941 gtaaaacctt tttcatcata gtttattcct aaaattcaac attaattaaa caccgatttg
    27001 acacacaggt ttaattaaac ttgcgttaag aaatacaaaa aaagttaggg gcttacaaaa
    27061 aatgtaaacc cctaaacata accaacctaa actaatacct gaaatcaggc attatgctgt
    27121 ttattgatct tcgtactcct taatgatatc cttaactcca tcgggtgaaa cacggccata
    27181 caccttttca ccaatcagca ctaccggagc aagtccacaa gcaccaacac agcgcaggca
    27241 gtttaatgaa aacttgccat cgggtgtggt ttcacccacc ttaacgttaa gtacccgttt
    27301 gaattcatcg agcacctttt cggccccacg aacgtagcat gctgtacctg tacaaatgct
    27361 aatcgggtgc ttacccttag gtatcatagt gaagaacgag tagaacgtta caacaccata
    27421 cacctttgca accgaaatgt ttaactcggc agccactact tcctgaacct cagcgggtag
    27481 gtaaccaaag gtttcctgag tcttatgtaa tacatttatc agttcgcccg ggtcgttgtt
    27541 aaacgacttg catatttcct taatcttggc tacctgttcg ggtttaagct caagctttat
    27601 atgtgacatt tgtgtttcct ccctatttaa tgtagatatc ctttttcttg ctgaaataat
    27661 gagtatgtag taggtggtgt gccttttcgc tcatgggctt gcctaggaac tcctcataaa
    27721 gttttactat gtaggggttc tcatgcgatt tgcgaattgg tttaccagca tcctcacggt
    27781 aaatagcctc catacgagct ttgataatgg cagagttacc gtggtgcaat ggttgaccgc
    27841 caccaccaat acatccaccg gggcaggcca ttacctcaat ggcatggtaa tttgccttac
    27901 ccgagcgtac atcctccagt agcttgcggg cattacccaa accatgagca attccaatgt
    27961 ttattggcaa tccattgaaa tcgatggtgg ctgaacggat accctccata ccacggagct
    28021 ctgtaaaatc gatcttgggc agaggtttgc cagtataagc ctcatatgct gtacgaacag
    28081 cagcttcaat tacaccacct gttgctccaa atattacacc agcacctgtt gactctccta
    28141 acggacggtc aaaatcatca tcgggcaact cctcaaagtt gatattaaac tccttgataa
    28201 ggtgtgctaa ctcacgggtt gatattgaga agtcaacatc ctgattgcca tcaaccttaa
    28261 actcatcgcg ttggcactca tacttcttag ccaagcaggg cattaccgat acaaccacta
    28321 attcttcgcg ttttttgccc aacttctcag caaagtatga tttggcaatt gcgccaaaca
    28381 tctgctgtgg cgacttggcg ctcgatggta catcgagcat atcggtaaag ttgtgttcaa
    28441 agaaattaac ccaacccggg cagcaactgg ttagaatggg taacttaaca ttcttgtcgc
    28501 cgttcaggaa tcgggatagc ctgtcgagca gctcggtacc ttcctccata atggttaggt
    28561 cggctgcaaa atcggtatcg aaaacgtaat caaaaccaat ctggcgcaaa gcggcaacca
    28621 tcttaccagt aactaaggtt ccgggcttca taccaaactc ttcccctaga gccgcacgaa
    28681 ctgcaggtgc agtttgtaca agtacggtct ttgtaggatc ggcaatagca cgaataacct
    28741 ttgtggtatg gtcaacctcg gttaatgcgc cggtggggca cacagccacg cactgaccac
    28801 agtaggtaca gggcgatttt tccaaatcct gctcaaaagc tggtgcaaca acagccataa
    28861 aaccacggtt tacagcgcta agtgcaccaa cggtttgtac ctcattacac atggtctcgc
    28921 agcggcggca catgatacac ttatcaacat cacggatgat agatggagaa aagtcctttt
    28981 tgtatgttga catctcagcg tattcctgac cggggatctc gcgaattccg agtttaatag
    29041 ccatatcctg caagtcgcaa cgaccactct taggacaggt taaacaatcc tttggatggt
    29101 cggaaaggat taactccatt acagtgcgac gtgcgttaat aacacgggtg gtatgggttt
    29161 taacaaccat accctcagcg cactcagttg cacagctggg agcaaggttt ctgcgtcctt
    29221 caacctcaac cacgcaaata cggcatccgg caggcttgtt ttcaactccc aaatcgtgga
    29281 gttccatgta gcaaagaaca ggaatatcaa tacccaactt cctggctgcc ttgtaaattg
    29341 tagtaccctt gggtacttca acctgtttgt tatctattgt aagttttact gtttccattg
    29401 ttatcaccct aattatttaa tgctgatagc atcgaattta cacttctcca tacatgcacc
    29461 acacttgata cagattgact ggttaatctc gtgtggtttt ttgcgttcgc cactgattgc
    29521 atttacaggg cagttgcggg cacaagccgt acatcccacg caagcctctg gatcaataac
    29581 atactgcatg agcgatttgc actgacccga tggacatttc ttatcaacca cgtgagccac
    29641 atactcatcg tagaagttat ccagggttga tagaacgggg tttggcgaag tttgtcctaa
    29701 accgcaaagc gaggtatcct taattacttg cgataagttg cgtaaacgct ctaggtcctc
    29761 catggtaccc tcgcccttgg taatgcgttc cagtaactcg tacagacgct tgttaccaat
    29821 ccggcaggga gaacattttc cgcagctttc ctcaacggtg aaatcgaggt agaacttggc
    29881 aaccgatacc atacagtcgt cctcgtccat aacaatcata ccgcccgaac ccatcattga
    29941 gcctgcagca atcaggttgt cgaaatcaat tggggtatcg aggtgtttct cggttaagca
    30001 accgcccgaa gggccaccgg tttgaacagc cttaaatttc tttccattct tgataccacc
    30061 acctatctca aaaattacct cgcgcagggt gatacccatt ggaacctcaa taagtcctac
    30121 gttgttgatt ttacctgcaa gcgcaaatac cttagtaccc ttgctctttt ccgtaccaat
    30181 tgatgagtac cagtcggcac ccttcaaaat aattggaggt acgtttgcaa aggtttcaac
    30241 gttattcacg ttggttggct tgcccatgta accactttgt gcagggaatg ggggtttgtt
    30301 gcttggttca ccacgtaagc cctccatgga gtggataagg gcagtttcct cgccacaaac
    30361 aaaagcgccg gctccgtagc gtaattcgat atcgaagcta aaactggttc caaagatttc
    30421 gcttcccagc aacccatact cacgagcctg atcgatggca acctttaggc gtttaatggc
    30481 aagtggatac tcagcacgta tgtagatcaa accctttgta gcgccaatac agtaaccgca
    30541 aatggccatg gcctccagca ctgagtgtgg atcgccttca agaattgagc ggtccatgaa
    30601 agcaccgggg tcgccctcgt cagcattaca tactacatac ttttgatcag catggttttt
    30661 gctggcaatt tcccacttta aaccggttgg gaatcccccg cctccacgac cacgcaagcc
    30721 agattttttg ataatatcaa tagtttgctg tggggtatat tcagtaagca ctttacctaa
    30781 tgcttgataa ccctcacgag caatatattc atcaatattt tcagggttaa tgaatccgca
    30841 gttgcgaagc gcaatacgca tttgcttgcg gtaaaaaccc atatgctttg aatcctcaat
    30901 ggcagccttc ttagtgggat caacatagag caaacgctcc accttacgac cctttactac
    30961 atgttccttt acaatttcgg tggcatcctc aggcttaacc tgtgtgtaaa atgtattatc
    31021 gggcaatact tttacaattg gacctttctc acaaaaacca aagcaaccgg tcataataac
    31081 ctgaacttcg tcctgaaggt tattggctgc cagctctagt tttagtttct ctgctattgc
    31141 atcgcttgag gaagctttac aacctgtgcc tccacaaatt agcaggtgca ttttatactt
    31201 tgacattact attacctccc agttggttat ttgttatcaa ttgactcgta gttaacggga
    31261 ataataccat caaccagttc accgttctta atgtatttct caataatctc atcggctttt
    31321 ttggtgtcaa catagccgaa aacaacaggg tcggcattgg gtaattttac ctcaatggta
    31381 ggttcggcat agcagtaacc catgcaaccg gtttgtgtta caacagcgtt tattccacgc
    31441 ttttccagtt cctcaagcaa aaaatccatt acattttttg ctcctgaagc aataccgcag
    31501 gtagccatgg caaccttaat ctgaacaagg ttctctgcag cttcgccctt ttccctaagg
    31561 tcgattttag cctgcaaggt ctccttcatc tttttcagat cggcaagcga ctttactttt
    31621 gtcataactt gttctccttg tgtgtatttg gttggtttcg taatattaac ctaataattt
    31681 tctgcaaaat taaattcaat tgttaagcga ttaacaattg aaaatatgtt tttcagctta
    31741 aattcgttat ttgtaattcg tctaaattcg aatagacata ctccttaacc agcgggtaaa
    31801 ccgatggctc gttcaacgga accccatcaa gcgcttggcg taactccgat gtagacaccg
    31861 caaattcggt tgtatcaaca accacgctta ccttgaactc aatatcaggg taggaacaaa
    31921 ggagcagcgc aatggtgccg cccaaatcgc ctaagggtaa acaatcgatg tgcgacttaa
    31981 cgaaacgggc tttaagggca gtgcctactc ccggttctga atcgatactg aatgctcctc
    32041 cggtaagttc agcattctgc tttagcaatg ggattcctaa gcccacctta cgggttgttc
    32101 gggttgtggt aaaggggtct actacccgct tcaaaaattc ttgctccata cccttgccat
    32161 tatccttgac agtgagctca atataatcat ttgcggtgtt aatatcaagg atgatttgta
    32221 tcaccttggc accggcaaca accgagttct gaacaatatc aaggatgtga agcgataagt
    32281 ccttcatttt ccaactgtta atttatatcg attttaaaca aagtgacaaa gctatatcac
    32341 aggcaatacc gttgaactgc ctgtatttag tatggagtcc ttaaaacact taaacgataa
    32401 gttttccaga ttatactctg tgtaggaagt tcctatttgg ctaagaaaat gcgagtcgga
    32461 atttcggatg aattgcgtgt tgggttttat ggggttgttt cgcttgaaag aatctactgt
    32521 ggtatttctt gaaatctcaa gtgcatcgta acttagcgat ggcggcacaa agccaagctg
    32581 actagtaaca ctatacttag gtctatcaac atgagcaggg ataaaaaccc cacctatccc
    32641 atgaacaaac tcggcaatct catcgatact ttttgccagg gctgcatgta gaaggtagtc
    32701 aatctcctga actatttcgt cgtgttcatt taccactacc tgatagccaa aaatatcggg
    32761 gttgttaggg atgcgggtta tgttttgctc tatgaatgcc tgcagtaact ggcgctgatc
    32821 ctcagtgtcg accaggcaaa ggcagtgtat ttcttcacgg gtattaacct cggcaccgta
    32881 taggcagtaa atgccatgct gttcagccag ggttcggatt agagggccat ggagggtgct
    32941 gttatgatcg gtaaccgcaa taagtttcag acccatttca gcggctcgca ttacaatatt
    33001 caccgggctc atttccaggt cggcacatgg cgatataacc gagtgaatat gtaaatcggc
    33061 cttaactatc attttttgtt gatgagatta tacagctggc cgcttatctc aaaggcttgc
    33121 aggttggttc ccaggactgg aatggcctcg ttgttactct gtgccatggt gttagcttca
    33181 ggctcaagac ctttgacaag aataacagcc gaaatatctt taagcgtggc aacggccatt
    33241 acattaacat gagtttgaag cgtaatccat acggaaccgg caggggcatt ccccataaca
    33301 tcgctcagca aatcggatgt ataccctcca actacctcct tgtttaaacc ggcctcaccg
    33361 gagtaaacct tcaggtttaa tgcatcaact atatctttta ccgtcatata ttgatatttt
    33421 tacttgttcc taatgggttt gtcgcaatcc ttgcggaacc ggttctcacc ccaaattctt
    33481 ttcatgatcc ggaatgagtg ttcagggtca agcttgtttt gctccatgat tttttgtagg
    33541 aaaacgcatt gcgaaaccga tgcgctaccc tgtgcaacat cctcggcaag cgcaatgcac
    33601 gaaggtgcgc cgcaaacccc gcagtcgata ccgggcagga agcacatcag ctggttaaca
    33661 cgctgcatct taagcatggc ctttgccata tcctcatcga gcttcagcat cgatcttggg
    33721 ctaagcgggc tcagcgcagt tcttccgcta atgcaagcat cgtattttac tatcttatcg
    33781 ttggttttat tgatactcca aaccgatacc ttacttgcgt ttttgcgaat tagctcggca
    33841 gctaaaaacc gattccctgg cgcaaggata ccacccgcac agctctcatc gcatgcgcgg
    33901 agctcaaggt aatccacatc ttgaatttca tcgttctcca ccttctccag aaactcaata
    33961 acattccata ttccatcaat agccaggcat cggccatcga tacacgatgc ttcgccgttg
    34021 gttagcgccc aagagtaact ccgggggcta agatccgaaa tgatttttgg ctcatttccc
    34081 gtaggtaatt taccttttat ctcacgcaga attcggttat agataaaatc catgttgata
    34141 atcccatcta tctccgactt ttcttctccc tcgggatgtc gtacagcaac tattttggca
    34201 gcacaagggg taatgtagaa tattccaatt tccttttcag gaacaccttg ttccaaaagg
    34261 cttgtcttta tatatgttgc ggtaaggtcc aatggcggtc ttatcagagc aatattctca
    34321 acaagcgagg gaaacttaac ctgaattagc cggactattg ccgggcaaaa tgatgatatt
    34381 accggtttaa cctcggttga agcaatgtag ctgttcagct tgccaattaa atattccacg
    34441 gaatcctcaa cctcaacaat ggacgtaaag ccaagactca gtaatgcttg cgcaacatca
    34501 tcgtgtgata tctcctttga aaactgccct agtagaacgg aaggaataag ggcaatgcga
    34561 tgtttatagg aaaaaatttt actgaaatcg tcctgttgaa ccgtaatggc atgggttggg
    34621 caaaccctgt aacactcacc gcagtcgata cagcggttgt ttattagctt agcttttccg
    34681 tttacaaccc ttaaggcctc ggtggggcag gctctcatac agtgcgagca gccgatgcac
    34741 acatcgggaa gaatttttaa cgcatggtag aattccaaat cagccacagc tatcctccgc
    34801 tactccttaa ggttaacctc aaactccaac cgtgttccta cgccaacaac gctagcaatc
    34861 ttcattttat cggtattctc tttgatgttt ggaagtccca taccagctcc aaaacccatt
    34921 tcccttacaa ttggcgatgc ggtagagtat ccgggtttca tggccaaatc aatatcgggg
    34981 attcctggtc caacatcatc gagcactaca cgaatcttat cggtctcaat atcaacatat
    35041 attttaccct gaaaggcatg agcaacaatg ttaacctccc cctcgtacag ggcaaccacc
    35101 acccttttaa taatgttagg ggaaacattg agctgcttta aaatcttttt cacctggctc
    35161 gatgcttgcc cggccctaac aaaatcgcct ccctggaccc tgtactcaaa gtgcatatgc
    35221 tattaataaa ccggttttac acccttactg taaagaatgc cggaagccct gaacatggtg
    35281 aaagggcttt cgataagaac aatgccatta tcctcggcca gctgaatcat atcctcgcta
    35341 atggttttat tgcgaacaaa tacaatggct ggtatatcgg ccatctcggc ggttctaacc
    35401 gcctgcatgt tggccaaacc tgtaattagc aacaatttgt cagcgcggac agtaagcaca
    35461 tcgctcatca ggtccgaggc aaaagcatac tcaatttccc tatcgagcat tgcgtggccg
    35521 caaaccacat taccatcaac cagctgggct aattccccta ttttcattcc taccgtattt
    35581 tgtgtttaca ccaaatcctt gcaatagcaa ttccgttaca aatttacgtt attctattaa
    35641 cattgttaca tttataacag atgcttaaga acacaaaaca tcaaaacgct accacctatg
    35701 caagatagct gaaaaagggg agaaaaagaa atcggtagcc ggggaatggt ggaaccgtga
    35761 accgtgaagc gtgaaccgtt aaacggagta tacagaatgc gatgatgtta gaggaagtta
    35821 gaggaagtta aatgagtaca aagcgtgaac cgtgaaccgt gaaccgtgaa ccgtttaacg
    35881 aaatcgaaca accagcaaca atcaacgaac aattgtattt gtttcacaga gtaacacagg
    35941 gttaaacaca gtgtttcaca gtgtaatcag ttaaatctgt gccatctgcg ttcaatttca
    36001 acccatgaac aaggaacaac gccccctgtc attccgagca aatgcgagga atctcattat
    36061 caagaggata ttgaaggag
//
LOCUS       DAOOWJ010000014        35914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1214_length_35914_cov_7.996598, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000014 DAOOWJ010000000
VERSION     DAOOWJ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 35914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35914
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_35914_cov_7.996598"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLW22_02660"
     CDS             complement(<1..1136)
                     /locus_tag="PLW22_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73637.1"
                     /translation="MKNIGMVLLSFLITIGAMSQSPRAFNYQGVIRNSSGEVLANQQV
                     TFRFSLTNNDASTVYYSESRISATNGLGIVNVPIGLGDIIQGNFSEIPWESGDIYLKV
                     EIMPEGSAGFTDMGIQRLLSVPYALFASDGISMKWLGSLPNPPAFPSRNEAYYNTMNK
                     ASFIWDGDSWEILALDGLPGIQGAQGDQGPAGPKGEPGEPGLDGISLIWLGSFDNEPI
                     APTLNNAYYNTTTKKSYIWDGDSWEIIAQDGEVGPVGPQGPQGLQGPQGQPGSNGISL
                     IWLGTLADAPSNPSINQAYYNSTEKKSYVYNGVGWDIIAQDGAIGPQGPQGEQGPQGD
                     PGVNGISVQWLGSLASAPGSPQLNQAYYNTTSKISYIWDGDTWE"
     gene            complement(1184..1684)
                     /locus_tag="PLW22_02665"
     CDS             complement(1184..1684)
                     /locus_tag="PLW22_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU73638.1"
                     /translation="MKKIYTVILSLFTLTAWSQDIPMQVIAAGGGYFEDSSAGLSISW
                     TLGEVAYTTLTSADFILTQGFQQGNLFTTSVDKPGITQNDITIYPNPARDYVKVKFEI
                     PNASGKATFDLYDITGRKVISKVMNVEQSVPAELNLSEIRSGIYMLKVTLEKDNLNRI
                     VKLVKE"
     gene            1809..2516
                     /locus_tag="PLW22_02670"
     CDS             1809..2516
                     /locus_tag="PLW22_02670"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOU73639.1"
                     /translation="MRERALDLNEEFKGKDALQVLTWFLKEYKGRIALSSSMGAEDQV
                     LTDMVMRIDPEVKIFTLDTGRLFYETYELIERTSLRYKKNIEIYFPSPAEVERMVTEK
                     GINLFYQSIENRKECCRVRKIEPLKRAFKGLEVWICGLRRNQSATRTENQMVEWDEAN
                     GLIKLNPLIDWSEQDVWEYIKANGVPYNPLHDKGFPSIGCQPCTRAIEPGEDVRAGRW
                     WWENPETKECGLHNRKK"
     gene            complement(2543..2613)
                     /locus_tag="PLW22_02675"
     tRNA            complement(2543..2613)
                     /locus_tag="PLW22_02675"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2579..2581),aa:Cys,seq:gca)
     gene            complement(2668..3024)
                     /gene="folB"
                     /locus_tag="PLW22_02680"
     CDS             complement(2668..3024)
                     /gene="folB"
                     /locus_tag="PLW22_02680"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOU73640.1"
                     /translation="MAIIEIENMEFYAYHGCYKEEQVVGNHFQVSILIEVETQAAQLT
                     DNLNDTVNYQVVYNLIKEEMQLKSKLLEHVAERIVSAIHNNFKNVERVWVKVSKLNPP
                     LGGKVEKVSVSLERKF"
     gene            complement(3055..4146)
                     /locus_tag="PLW22_02685"
     CDS             complement(3055..4146)
                     /locus_tag="PLW22_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU73641.1"
                     /translation="MNAIVQKILQTNDYVSLPGLGSIVRKYESAQLTNDGKTLLPPKE
                     YFVFDPNRTFNDEALENYLEASYGLSKSEASRRVEEFCQTLLNSLKQNKTVSIPGIGS
                     LKTGVDGGIELIADDALATTFLPPVDIVVNHQGTEKVEANQDLGSSMPTYRKEDKSTP
                     TVNVEATPSYIPGTPKKSQRSFAAGLFAAVMVIGVVAAFIFISELRFWEGNKSESVSQ
                     VAVVVPDESIKPSNYEQPVSEVKSDSASVQNMATDSSINAFPRSEVKQHTSVIVPIDK
                     KSALLFQDKPIEESKTFYIVIGSFASRDNAQKLIDEVSRLGYKPFILPGNNVYRVVIY
                     QFTNRDRALRELERVRGLHLTSQAWLLTL"
     gene            complement(4955..6256)
                     /locus_tag="PLW22_02690"
     CDS             complement(4955..6256)
                     /locus_tag="PLW22_02690"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOU73642.1"
                     /translation="MKRNVLLLSIFMLGTMVAMADYNPANNGSASKMKTGNRNASTTT
                     DAPFYNPAGTVWGNDGFALEFSTIPFYSTKSIFDDGNFIGNILEYKSKTSSLLYPALN
                     LTYKKDRFALFMFAGITNGGGAGNYNDGLPTFERLGYFNVITGIMGGLLPGAVTDYGV
                     KTSFKGSAYGVGGWLGAAYRVNDWLSVGGAFQYSRQMNHQEGWLDITYTPAPSAVPIP
                     RTEIDVDFTGQSFGFIGSVDLKPMPNLLIANTFRYFTEMELETKVNDNKSGGLFTDGD
                     KVKSTYVPTYNLGVAYNANEKLTLHYNMNVSLYSMLSLDEDVDGVNVAEYYHNGIDFG
                     VAAEYQFTEKFNWGVGCTYAPYKMKRQYQSEMEFENNTIWLNTGASLKLKENLGIDLG
                     FQVGLATQERKVESTEWVAPFNQKYENGPSYSMGIGFWYKF"
     gene            complement(6799..8832)
                     /gene="metG"
                     /locus_tag="PLW22_02695"
     CDS             complement(6799..8832)
                     /gene="metG"
                     /locus_tag="PLW22_02695"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOU73643.1"
                     /translation="MNSFKRYLITSALPYANGPVHIGHLAGVYIPSDIFTRYLRAKGV
                     EVISVCGSDEHGVPITIKARQENTTPQAVVDKYNAIIKDSFERFGIAFDIYSRTTSKV
                     HYDTASEFFRKLYDKGVFIEKSTEQYYDEEVGQFLADRYIMGTCPHCGNDRAYGDQCE
                     KCGTSLSPTDLKDPRSMLSGSKPVMKETKHWFLPLDRFQPDLEKWILEEHKEWKPNVY
                     GQCKSWLDQGLQPRAVSRDLDWGVPVPVEGATGKVLYVWFDAPIGYISATKELTPNWE
                     KYWKDPETRLIHFIGKDNIVFHCIIFPAMLKAEGSYILPDNVPANEFLNLEGDKISTS
                     RNWAVWLHEYLDEFPGKQDVLRYVLTANMPETKDNDFTWKDFQTRNNSELVAILGNFV
                     NRAMVLTQKYFDSKVPAIAELDNYDRETLAEIPKLKQSIEQNLDEFRFREALKEAMNL
                     ARLGNKYLADTEPWKVYKTNPQRVATILNISMQITANLGVLLEPFLPFTAEKIRAMVN
                     LNGKDWNLAGGDNLLLPGHQLNEPSLLFDKIEDDVIERQLEKLRKAKEANERANIKVT
                     PLKDSVTYDEFSKMDIRIAKVLEAERVPKTQKLLKLKIDTGVDTRTIVSGIAEFFTPE
                     QMVGKQVVVLANLEPRTIRGIESHGMILMAEDVDGRLQLVIPADSVSNGSTVK"
     gene            9075..9998
                     /locus_tag="PLW22_02700"
     CDS             9075..9998
                     /locus_tag="PLW22_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOU73644.1"
                     /translation="MENITPPYLQPGDTIGIVAPARSITRDELQPAIRFLTSQGFLVK
                     TAPHLYSKHYQFAGTDGERAQDLMEMIEDQSVKAILCARGGYGSVRILDLLNLRKLQH
                     NPKWIIGYSDITFLHSLITGWFGIETLHATMPINFPDSVQGNQSTMALISALKGETLY
                     YELKHHPLNLPGHATGVLIGGNLSILSSLSGTTSDMSYSGRILFIEDVDEYLYHIDRM
                     IMQLKRSGKLKSIAGLIVGHFSDMKDNSEPFGSDAYEIIYSAVKEYRIPVCFGFPAGH
                     AELNLPLIIGRTVELKVSDEGAKVGFYRSLT"
     gene            complement(9990..10628)
                     /locus_tag="PLW22_02705"
     CDS             complement(9990..10628)
                     /locus_tag="PLW22_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73645.1"
                     /translation="MRFICYIAFFTLLLSTNAYSKVGGFEGKVNIVREGVYDTIQVEV
                     YIKGNLIRVNEFSKGGKLIRSYFANVESGSTYLVSFSNRMYSKVKVDNSPAISNVSIL
                     KSENYIEINGIRCYQWRVKNLATQTETTYWVSETKYDFAPKLFSALANAGSAIEELVS
                     LPQPEGILPLMVVDRTRFRKLRSNIRVLSITPMQISQTLFTIPVGFNELISS"
     gene            10692..11069
                     /locus_tag="PLW22_02710"
     CDS             10692..11069
                     /locus_tag="PLW22_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOU73646.1"
                     /translation="MVEKIGGNKDLGSKGEEIAASYLAAKGYKILHRNWHFGHKELDL
                     VTQLNDTLVIVEVKTRSTHYWEEPKESVKRKKQRMIVEAADEYVQRHNLDCDVRFDIV
                     SIVINHGLPPEIEHIEDAFFPTL"
     gene            11090..11443
                     /locus_tag="PLW22_02715"
     CDS             11090..11443
                     /locus_tag="PLW22_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOU73647.1"
                     /translation="MNIELIREHCISKPDVTESFPFDDDTLVFKVNGKIFAMVNLSGD
                     TQLNLKCDPELAMELREKYMAVQPGYHMNKRHWNTIAIDGSIPDALILDWIDHSYNCV
                     CLNKGKRKLGSRKKK"
     gene            12446..13291
                     /locus_tag="PLW22_02720"
     CDS             12446..13291
                     /locus_tag="PLW22_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73648.1"
                     /translation="MKPVVHLLTVLLLAISVSVQAQESFIVTRINGVVTNNKTGTQLK
                     AGDVLQPSDKVTFDSFDSYVISIGQNMGRYMIKLHEPPAPETIQQLSAMVKDIAVPTK
                     RRSLMAERYRPDETTVSDLRSYFGNDKFTIIGDNIAVPINPQSYPIDENKFVVFYYRV
                     NNNPVSKKIGYENTTLFIERDKLVNTNAGVITQNEIPSVAVYLYERNTKTSEEITKFN
                     LAFVDKETVTNEFMTIIPILVKQKMGKDEIKKYLIEYFYDFYGATDSRSIKAFVDELV
                     DTAIK"
     gene            complement(13376..14371)
                     /locus_tag="PLW22_02725"
     CDS             complement(13376..14371)
                     /locus_tag="PLW22_02725"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOU73649.1"
                     /translation="MKSRWIIILAAAVALSPSCKKGSKGERSLLPNVTGSANELVVIL
                     PKALHSDSLGKLYSDILTEDVPFLPQSEPRFDMVMIPPEAFTDIFKPHRNIIFNDIVK
                     GAKEAKMSLKRDVWAAPQTILYISGPSYKSIYDLLLKERDKVVSIFEQAERDRVIQNA
                     ITYEEKPIREALEKKYGVSMYFPKGYRLNFDHDTIAWVSYETPLTSQGIILYTFPYTA
                     KNTFTVDYLLAKRNEFVKTISGPTQGSYMTTSSVIVPQLTDMMFKNRYYGVMRGLWDV
                     YKHPMGGPFISHITVDEERQRVIVAEAYVYAPSKEKRNYLRQVEALLYTMEIIKK"
     gene            complement(14438..15547)
                     /locus_tag="PLW22_02730"
     CDS             complement(14438..15547)
                     /locus_tag="PLW22_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU73650.1"
                     /translation="MSFIDAIENLPIFVPTNQTDTARQKPDLYFECQLAEIGRNSPID
                     FDYNPHVRRYIEIFTIERREQISKMIGLANYYFPLFDDVFSKYQLPLELKYLAVVESA
                     LNPLAISPTGAVGLWQFKINTSKMFNLQVNNFVDERMDPLKSTEAAAQYLQYLYRTFN
                     DWSLALAAYNVGPGPIRNAIERANGERNFWKLYPLLPESAQNYVPAFIAAAYVMNNYH
                     EHGITPAPVALAQNQTDTVWVNKPLDLNLMAKAIDIDINIIRFLNPTYRYDYIPEGSS
                     PQVLRLPADKTGLFIRKSKELYLNTSKPKILPALAADSTLTRKVYTVKNGDSLHKLAM
                     QFGCTLQDIYRWNPGLDSTITAGQNIVIWVNPKNL"
     gene            complement(15653..15826)
                     /locus_tag="PLW22_02735"
     CDS             complement(15653..15826)
                     /locus_tag="PLW22_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOU73651.1"
                     /translation="MLTLFIGGGELLVIAIVILVIFGASKIPIFMRNLGRGVGEFKKG
                     IKEAENQEDKEKE"
     gene            complement(15838..17157)
                     /gene="rseP"
                     /locus_tag="PLW22_02740"
     CDS             complement(15838..17157)
                     /gene="rseP"
                     /locus_tag="PLW22_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOU73652.1"
                     /translation="MEILIKAGQFFLSLSILVILHELGHFLFAKLFKTRVEKFYLFFN
                     PWFSLFKIKRGETEYGVGWLPLGGYVKISGMIDESMDKEQLKQPPQPYEFRSKPAWQR
                     LLIMIGGVLVNVILALFIYSAVLYTWGEDYLPTKSLKYGVSCDSLATELGFRNGDKIL
                     YVGGKEIENFQQIAHDILLNENRDVTVLRGTDTLNIKIAEDYVAKLVKSPIIFFPRIP
                     FVVDDVKSGEPAEKAGFIKGDRLLALNGQSAQFFDEFKSEMQRSKGKTVRVTVERNGN
                     PIDLNVDVPETGIIGVYPVADLGKFFELSHREYSFFHSIPAGVKKGFATISSYLKQLK
                     LIFSPKTKAYESVGGFITIGKIFPSTWDWQAFWSLTAFLSIILAIMNILPIPALDGGH
                     VMFLLYEIITGRKPGDKFLEYAQLVGMAILLALLLYANINDIVKLFR"
     gene            complement(17142..18314)
                     /locus_tag="PLW22_02745"
     CDS             complement(17142..18314)
                     /locus_tag="PLW22_02745"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOU73653.1"
                     /translation="MKRIAILGSTGSIGTQALDVIREHPDIFSVELLVAQNSWELLVQ
                     QAIEFQPNTVIIANQSHYQRVKEALANHPIKVYTGKQSIVESVVSSEVDMVLTAMVGF
                     AGLEPTLSAIEAGKAIALANKETLVVAGELVTKAAMQKRVPIIPVDSEHSAIFQCLVG
                     ERSPIEKIILTASGGPFRNHSLDQLMHVSKADALNHPNWCMGQKITIDSASLMNKGLE
                     VIEAKWLFGLNPEQIEVVIHPQSIVHSMVQFIDGSIKAQMGLPDMKLPIQYAFTFPER
                     VSSGFPRMDFSMSHTLTFEKPDTNRFPSLALALESLRAGGNMPCVLNAANEVAVDAFL
                     REQISFMAIPKVVEGAMQAIPFIGQPTFADYIQTDSETRIKSVELINKNIIKWRYS"
     gene            complement(18323..19183)
                     /locus_tag="PLW22_02750"
     CDS             complement(18323..19183)
                     /locus_tag="PLW22_02750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU73654.1"
                     /translation="MAAKKKVKIFKNLWNKYRLSIFNEENYEEVWQLRLSRMNVIVFF
                     GGFSILLIALVSVLIAFTPLREFIPGYPDSQTRKGYVKNALKVDSLERVVAQWQRYYE
                     DINILLNGGEPLTGRQTVIDTVKPTAKVELSPSEEDSLLRMQIESDEMFNLSVGSSTT
                     KKSNIPFFIPPVKGIITNKYNPSKNHYGVDLVAGPNEGVLAIAKGTVILSTWSLETGY
                     TIAIQHEEGFLSVYKHNSKLLKRQGEFVNSGDVIAIIGNSGELTTGPHLHFELWHHGA
                     TVDPAKYIKF"
     gene            complement(19331..19936)
                     /locus_tag="PLW22_02755"
     CDS             complement(19331..19936)
                     /locus_tag="PLW22_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73655.1"
                     /translation="MLQRLSLILSIILHPILATTYIFIVIFSSNSFVAFLPVRFKVML
                     TVFVAINTIFLPLLLIVLLHRLRIIHDYTLKASRDRVFPLAISVLPYLFTIFLFSRLQ
                     VPFVLIKILQAGIYTLMISAAISYFWKISLHLTGIGGISGFLLASALQGNQAAVPIFI
                     VSILLSGLLASARLIKGDHNPTQVYTGFLTGFTLVFLIFFK"
     gene            complement(19951..21408)
                     /gene="rpoN"
                     /locus_tag="PLW22_02760"
     CDS             complement(19951..21408)
                     /gene="rpoN"
                     /locus_tag="PLW22_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOU73656.1"
                     /translation="MLKQRLQQKLLQKLSPQQIQMIKLLEIPTMLLEQRIKKELEENP
                     ILEEVEQTEPQTEPEIVLPDDNQEGNDDEFSIEDYMNDDDIPSYKLYSSNASPDDKRE
                     EIPFSTGISFHEHLENQLGMADLTEREKQLGFYLIGNIDEDGYIRRKLTAIADDIAFS
                     LGIETNEDELERILYVIQEFDPPGVGARDLQECLLIQIKAKEQQNEITKIAYKILKYH
                     FDEFIKKHYDKIASKLNLSDEKLKLAIDEILKLNPKPGGSFTDPYDQSAQHIIPDFIL
                     DYEDGEFRLNLNSRNVPELRISKEYADMLESYSYNKEFSNSQEREAIAFVKQKIESAK
                     WFIDALKQRQNTLLTTMQAILDYQKEYFRDGDESKLKPMILKDVAEKTGLDISTISRV
                     ANSKYIQTHFGIFPLKHFFSEGMQTDSGEEVSTREIKKILAECVDNEDKRNPLTDEEL
                     MEILKGKGYPIARRTVAKYREQLGIPVARLRKELN"
     gene            complement(21415..22812)
                     /gene="asnS"
                     /locus_tag="PLW22_02765"
     CDS             complement(21415..22812)
                     /gene="asnS"
                     /locus_tag="PLW22_02765"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOU73657.1"
                     /translation="MRNSERTKVKLLLESGVVGAEVAAMGWVRTKRGNKNVAFVALND
                     GSTIHNIQVVFDMTRFTEEQLKGVTTGACIAVRGLLVESPGSGQRVEIQANYLEVLGE
                     ADATTYPLQKKGHSMEFLREIAHLRPRTNTFGAVLRIRHAMAYAIHKYFNDKGFFYIH
                     APIITGSDAEGAGAMFRVTTLDLENLPRTEDGKIDWNEDFFGKEAKLTVSGQLEAELG
                     ALALSEVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDIQDNMDLAEDFLKYLIRYA
                     LEHCADDLEFLNKMYDNELIDRLKFVIENPFERLTYTEAIKILEAANHKWEFPVFWGA
                     DLQSEHERYLVEKHFKKPVILTNYPKEIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGG
                     SQREEVYDKLLSRIHELHIPEKDVWWYLETRKFGSAPHSGFGLGFERLLLFVTGMTNI
                     RDVIPFPRTPKNCEF"
     gene            22919..24406
                     /locus_tag="PLW22_02770"
     CDS             22919..24406
                     /locus_tag="PLW22_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73658.1"
                     /translation="MNEKFFERWGATFVATLLLLIYILGISNLSTIDGWGYAADIVNS
                     SNLLRPHHLLYTITGFYWVKIWHFFLPNAETIYLLKLMNALCASVTAFILFKLLRLVK
                     VDTIQTTIYTIIAGLSWGFLRFSIDNETYTIPVMLAIWASYLFLKAESSPKISNMVYS
                     GLLSASACLYHQIMFFWWIALAIGTIRKLNRRDYKPLLFLAPAVIVPAAYILAMLSMG
                     IEINFTSFFRFIFSDYYTGNASIELNSTILMVGIINLFRTFFQMHGYINYLLAQSWLW
                     LIPLVVSGIAFAFFSFWLVRYTKPTRLEKFHKIHLLALVLQVGFAFMAGGNAEFMAMV
                     PFLTIITATGLSIKRDATVWFAISLSVWNISFGVLPQKIYQADGSDLVIKSITQNTNE
                     RRAYILTDAPKVINRLEYLQIQKPLIVKEVNIEPQKIYATLDSLLLQGYTIYTDCINQ
                     PRVTSRATLITDVSLEWDKLEGYKTTSIDSLEMLGGKVYVTKISR"
     gene            complement(24409..25272)
                     /locus_tag="PLW22_02775"
     CDS             complement(24409..25272)
                     /locus_tag="PLW22_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU73659.1"
                     /translation="MKHIIVPIDFSKESLNGLRLAIIFANRFDSMVQMVHVQKYPTDL
                     TPGQFEEENKKIEKKLEALVEEYSTKMSNSANLSYIIKKGKIYREVVNQAHAFEDSAI
                     ICSTHGASGFEEFFIGSNAFRIISATQCPVITIRHGAMPRDIQKIILPIDVTADTRQK
                     VPITTEIAKVFDAEVHVIAVATMQTDEIDLKLNAYTKQVCSYLKEHEVRFQKASLRGD
                     NITDITIEYALDVNADLISIMTEQNIALSDFVLGSQAQQMLSRSPIPVLSVTPKEIFI
                     MGSFKTYGSGY"
     gene            25450..26520
                     /locus_tag="PLW22_02780"
     CDS             25450..26520
                     /locus_tag="PLW22_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU73660.1"
                     /translation="MMTRKRIGIWILLFSVALATLTLASRPNPTEESEKEKPAKGTAI
                     IITGAAAKIAQEAALLESLYERGELNDVVFISGASSGALNAVMLNAVLEKKLTWKEYR
                     ELFGDIKNDNVFRTDKKGLPVSNEPLRKLLEEIVVNRLGYKTLADLPFPTAISVVSLR
                     PSPLSEGTYRFCNRKINRESDSTLNLVDVLMASTAYPLVFPPSRINGIKAIPDIPYYD
                     GGIGDDRVPFQALIEFENYRKQPVERVIIISRKRDTDMTLADELKPFGYELKTKLNNG
                     LSPDVLTSLGFWRRLEHIKKRYPELAAKTLVFVPSYRYSFPMFDFNNLKEQYELTYQW
                     AKQNKPIPLNEFLKQKPTLNKP"
     gene            26552..27541
                     /gene="trpS"
                     /locus_tag="PLW22_02785"
     CDS             26552..27541
                     /gene="trpS"
                     /locus_tag="PLW22_02785"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU73661.1"
                     /translation="MDIVLSGIRSTGKLHLGNYFGALRNFVRMQHENKCFFFIADYHS
                     LTTHPTPEDLHGSVKQVLAEYLACGLDPEVATLYIQSDVPQIPELYLLLNMNAYKGEL
                     ERCTSFKEKVRKQPDNINAGLLTYPTLMAADILIHKATKVPVGKDQEQHLEMTRTFAN
                     RFNRMYKVDYFPEPVAFNFGEDLVKIPGLDGSGKMGKSEGNAIYLSDDPETIRKKVMK
                     AVTDAGPTQPNQPKPEAIQNLFTIMKAVSSPDTLQYFEDKYNACEIRYGDMKKQLAED
                     IITFTTPIRERIEAILADNDYLRKVALMGAEKARESASKTIRDVRHIIGFREF"
     gene            complement(27562..27876)
                     /locus_tag="PLW22_02790"
     CDS             complement(27562..27876)
                     /locus_tag="PLW22_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOU73662.1"
                     /translation="METFGISEKSYLLIIDVLKSYPEVETAIIFGSRAKGNYKNGSDI
                     DLALIGESLNSKIILDIAGILNEGIPIPYHVDVVNYHSISTPELKEHIDRVGKEFYKK
                     GC"
     gene            complement(27876..28265)
                     /locus_tag="PLW22_02795"
     CDS             complement(27876..28265)
                     /locus_tag="PLW22_02795"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOU73663.1"
                     /translation="MDIRWKQRFDNFEKAFLRLKDALRNDSPSELEKAGVIQYFEFTF
                     ELGWKTLKDYLQEMGVEARFPRDTIKEAFTNGLIQDGDLWLDMLEKRNQMSHTYDEFT
                     ANQSFQMVRDEFIHGIEQLYTKLKDLR"
     gene            complement(28313..29782)
                     /gene="cysS"
                     /locus_tag="PLW22_02800"
     CDS             complement(28313..29782)
                     /gene="cysS"
                     /locus_tag="PLW22_02800"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOU73664.1"
                     /translation="MSQIAIYNTLTRKKEVFEPLNPPHVGLYVCGPTVYGDPHLGHAR
                     PAITFDLLFRYLKHLGYKVRYVRNITDVGHLENDADEGEDKIAKKARLEQLEPMEVVQ
                     YYTDRYHSAMKLLNVLPPSIEPRASGHIIEQIELVKKIIDNGFAYERKGSIYFDVVKY
                     NKKHNYGILSGRNIDDLMANTRQLDGQDEKQNPFDFALWKKASPQHIMRWPSPWSEGF
                     PGWHLECSAMSTKYLGEPFDIHGGGMDLLFPHHECEIAQSVAANGKQAVRYWMHNNMI
                     TINGQKMGKSLGNFITLEELFTGTHRLLEQAYSPMTIRFFMLQAQYRSTLDFSNEALQ
                     AADKGLARLMAANRQLDRIKPSATSSVRVDLLKEKVYEALNDDLNSPVAIAVLFDWVR
                     QINLLAEGKETISESDLHELKSFFRTVIFDILGLVDEHTSGSKHAELTAKLVEMLLSM
                     RMEAKQRKDFATSDRIRDELAKLGVIVRDRKDGFDWELE"
     gene            30129..31232
                     /locus_tag="PLW22_02805"
     CDS             30129..31232
                     /locus_tag="PLW22_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU73665.1"
                     /translation="MNQLVRYIIIGFVVALVAFLIWYFSSIVAYILISAVLSLMGKPI
                     VDFISSIRIRNWSPPRFLGATIALITIWILFIAFFRVMIPLVIAQFNELGSIDVQSLV
                     ASFAEPINSIQQFIQNYLPASAQGFVLQDFLITKISGIFTVSMITNAFSSTANLLISL
                     VIALFSISFITFFFLKDDTLFFKSVILLFPEKYEMQITRALVSINKLLRRYFVGIVIE
                     STAIMVLDTIGLSIIGVRFQTAVVVGLIAGIMNVVPYVGPLTGTVLGTLIVLATQLHL
                     GFTANMLTFTILVVSVFLITQLIDNVVFQPLIYGNSVKAHPLEIFIVLLVAGSVAGIL
                     GMLLAIPSYTVIRVFAKEFFNNFRVVQKLTEKI"
     gene            31280..32209
                     /locus_tag="PLW22_02810"
     CDS             31280..32209
                     /locus_tag="PLW22_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU73666.1"
                     /translation="MIKDITSTAATIAIAMLLPCLSFSQNFEADSVFTWQLVETNNIG
                     SQSTLKFIPRTGVTPTGWDFGDSNTSTEVAPTHTYSYTSWDDSVTVTLNYTLNGESLS
                     HSREIPVSPAFFFVFPDRNLGRLASYKRIFLNAYRIPNHPDSLGNLRFTWTVDGSPLA
                     GNAFDDVALDQWPNIYYTFTTAGLHQVKLDVRNSNSGQTAEFTQTIRVLPDFTSGKVP
                     LENIPNVFTPNSDGVNDNFIVPTSGTAWFDFRIFSRSGGLIYKAESSVLVWDGKNNQG
                     TDMPEGVYYYVIEDLSGQYDSAKGFVYLFRGKK"
     gene            32216..32767
                     /locus_tag="PLW22_02815"
     CDS             32216..32767
                     /locus_tag="PLW22_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73667.1"
                     /translation="MNRIPQFILLFALLLTSCNNIVFNKPQPVDGKIITNLNNAFPGI
                     FISSDNDTLTITRNTISLGSGNKFTVTGTLGKNLDVREYSNGFVVNFSDSVKGRLVWI
                     AYIFKLSNDSLFISFSDFDPNKLTEVEKEIGNKVPYEKIKDENKENSKIILKPRNSRE
                     FDKLVSAGIFNKTISMKMEKPKP"
     gene            complement(32773..33369)
                     /locus_tag="PLW22_02820"
     CDS             complement(32773..33369)
                     /locus_tag="PLW22_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73668.1"
                     /translation="MLTIEQLIESYDKPGTVILLEGKRAVLPTDQHKLIEVGKLLAGS
                     SKHIVFRSGNAKGADYFFSQGVSMVNPSRLQQVTPYRGHRTKETLGRQTVSLDDVDLA
                     SEPNVIFHTKNSSNVSQLIDSFLSLGDNALTVKARYLLRDTIKVVGTSKLQPATLAIF
                     YDDLSKPKTGGTGHTMRVCEKNGIPYFDQRVWFKWVEQ"
     gene            complement(33378..34727)
                     /locus_tag="PLW22_02825"
     CDS             complement(33378..34727)
                     /locus_tag="PLW22_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU73669.1"
                     /translation="MNKIFLIMQREFMTRVRKKSFIITTLLSPIFFAAIAILPSYLAS
                     LEVTEVRTVAVVDNTKQYIDVIPSTEYIKVTYVTDTPAEKVKENLNETGYYALLVIDS
                     TSTPNQAAFTIYSQKQPAIDVMQHLENTLEKEIEARKLKTYNIDNLDKILADVKTRIS
                     IKSIKVSQTGEEKESNTLIAMAIAYIMSFLMYMMVLITGNQVMQGVIEEKTNRVVEVI
                     ISSVKPFQMMAGKIFGMASVSLLQILIWVVMTAALAGVGINIISSKMTTKPATEQMQA
                     MAQADPQMAQVQEQDTAVANSEGMSFLTALKNQNFPLLIGGFIFYFLFGYLLYAAMFA
                     AVGSAVESITDTQQLTLPITLPLILAIIVMISGIKSPDGPLAFWFSLIPFTSPVIMLT
                     RLPFGVPTWQLALSATLLVGTFILITWLAAKIYRVGILMYGKKYTWKEMIKWISYRG"
     gene            complement(34753..35694)
                     /locus_tag="PLW22_02830"
     CDS             complement(34753..35694)
                     /locus_tag="PLW22_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU73670.1"
                     /translation="MHLLEAKGIEKRYSNHLALDDVSISVPRGSIYGLLGPNGAGKTT
                     LIRIINQITGPDKGEVLFDGRHMQPNDIYRIGYLPEERGLYKKMKVGEQALFLAQLKG
                     LSRAEAMKRLKYWFEKFEIQAWWDKKIEELSKGMAQKVQFMVTVIHEPELLIFDEPFS
                     GFDPINVQLVKDEILELKRKGTTIIFSTHNMASVEELCDHITLINKSRNILSGQIDEV
                     RQSYGSNVFEIAFRGNPDSLRQKVEDRFELLKIDTDNSLPKAQVRLKSQSDANSLLQE
                     LIPAVEIVSFNPIVPSMHDIFIQVVKEYNQKHGLVIS"
BASE COUNT        10255 a         7868 c         7363 g        10428 t
ORIGIN      
        1 atctcccacg tatctccgtc ccagatgtac gatatcttgc tggtggtatt atagtaggcc
       61 tggtttaact gaggggatcc aggagcgctg gcgaggctgc ccaaccactg gacagaaatc
      121 ccattaacgc ctggatcacc ttgaggtcct tgttctcctt gcggcccctg tggcccaatg
      181 gcaccatcct gagctataat atcccaccct actccgttat aaacgtacga tttcttttcg
      241 gtgctgttgt aatatgcctg atttatactt ggattagagg gcgcatcagc taaagttcct
      301 aaccaaatca atgaaatacc atttgaacca ggctgccctt gtggaccctg taatccctga
      361 ggcccttgtg ggccaactgg ccctacttcg ccgtcttgag caataatttc ccaggaatcg
      421 ccgtcccaga tatatgattt ttttgtagta gtgttgtagt aagcattatt taaagttggt
      481 gcaattggtt cattatcaaa tgaacccaac caaataagcg aaatgccatc taacccaggt
      541 tctcccggtt cgccctttgg ccctgcaggc ccctggtcgc cctgagcacc ttgaatcccg
      601 ggcaaaccat ccagtgccag tatttcccat gaatcgccat cccaaatgaa tgaagccttg
      661 ttcatagtat tgtagtatgc ctcattacgc gacgggaagg caggagggtt tggcaatgaa
      721 ccaagccatt tcatcgatat accatcggat gcaaataatg cataaggaac cgataacaat
      781 cgctgtattc ccatgtcggt aaatcctgca gaaccctcag gcattatttc aacttttaag
      841 taaatatctc cactctccca tggaatctct gaaaaatttc cttgaataat atcgcctaaa
      901 ccaattggaa cgtttacaat gcccagccca tttgtggctg aaatcctact ctctgagtag
      961 taaactgtgc tggcatcatt atttgttagg ctaaatctaa aagttacctg ttggtttgct
     1021 aaaacctctc cactagagtt tcttattaca ccttgataat tgaatgctct tggagattgc
     1081 gacattgcgc caatggttat cagaaaagat aacaacacca tgccaatatt tttcatggct
     1141 aaaaagtttt ggttagactt ttaaaggcta taaaaagggg aagttactcc tttaccagct
     1201 taacaatccg attcagatta tccttctcca gcgttacttt tagcatataa atgcctgaac
     1261 gtatttccga tagatttaac tcagcgggta ctgattgctc aacattcatc acctttgaaa
     1321 taaccttgcg tccagttata tcgtataaat caaatgttgc tttaccgctc gcattgggta
     1381 tttcaaattt aacctttacg taatcccttg ccggattagg gtatatggta atgtcgtttt
     1441 gagtaattcc aggtttatca accgatgtgg taaacaggtt tccctgctgg aatccctgag
     1501 tcaaaataaa atcagctgat gtgagggtag tataggcaac ctcgcccagt gtccaactaa
     1561 ttgatagccc tgctgatgag tcttcaaagt atccacctcc agcggcaatt acctgcattg
     1621 gtatgtcctg cgaccatgcc gttagtgtga atagcgataa aattactgtg tagattttct
     1681 tcattttgta ttattttaaa tttgcataat gagcaagtta gtaaattata ataccaaagt
     1741 caattttttt acattaattt gaagttttgt atttctaatt tatcgtttgg agtgttaaaa
     1801 ttaaaacaat gagagagaga gcattagact taaacgaaga gttcaaaggg aaagatgccc
     1861 ttcaggtgct tacgtggttc ctgaaagagt ataaagggag aattgctctg agttccagca
     1921 tgggtgcaga agaccaggtg cttaccgata tggtaatgag gattgaccct gaagtaaaaa
     1981 ttttcactct tgacacggga cgattattct acgaaaccta cgagcttatt gagcgcacct
     2041 cgctgaggta caaaaagaat attgaaatat actttccatc gccagccgaa gttgagagaa
     2101 tggtcaccga aaaggggatt aatctgtttt accaaagtat tgagaatagg aaggagtgct
     2161 gtagggttcg caaaattgag cccttgaaaa gggctttcaa agggttagag gtttggatat
     2221 gtggtttgcg ccgtaaccag tcagctaccc gaaccgagaa ccaaatggtt gagtgggacg
     2281 aggctaacgg ccttattaag cttaacccgc ttattgattg gagcgaacag gacgtttggg
     2341 agtatattaa agccaatggg gtgccctaca accccttgca tgacaaaggt tttccaagca
     2401 ttggctgcca accttgtacc agagccattg aacccggtga ggatgttaga gctggccgtt
     2461 ggtggtggga aaaccccgag accaaggagt gcggtttaca caatcgaaag aaataaaaaa
     2521 gccccatttg ggggcttttt tgaggtaccg agcggattcg aaccgctgta cgtggttttg
     2581 cagaccagtg cctaacctct cggccacggt accctttggc actgcaaaga tacaaaaaaa
     2641 attaatcctg caaatttttg tgggttttca aaacttgcgt tcaagcgaaa cactcacctt
     2701 ttcaactttt cctccgagtg gtgggtttaa ctttgaaact ttcacccata ccctttccac
     2761 gtttttgaag ttattgtgta tagctgaaac aatacgctcc gcaacatgct caagcagctt
     2821 agattttagc tgcatttcct ctttaatcag attgtaaaca acctgataat tcactgtatc
     2881 atttaaatta tcggtaagtt gagccgcttg tgtttcgacc tcaattaaga tgcttacctg
     2941 aaaatgattc cctacaactt gttcctcttt gtagcatcca tggtaagcgt aaaactccat
     3001 gttctcaatc tcaataattg ccatcgttta agataaaatg tgtgtggagt atagctaaag
     3061 ggttaaaagc caggcttgtg aggttaagtg caaaccacgc actcgctcca attccctcaa
     3121 ggctctatcg cgattagtaa attggtaaat taccactctg taaacattgt tgcccggtaa
     3181 aatgaatggc ttatagccca aacgcgacac ctcatcaatt aacttttggg cgttatcgcg
     3241 gctagcaaaa ctgccaatta caatatagaa agtcttactc tcctcgattg gtttatcctg
     3301 aaataaaagg gcactttttt tatcaattgg aactattaca cttgtgtgtt gttttacctc
     3361 gctccttgga aaagcattaa tggaactatc ggttgccata ttttgaactg aagccgaatc
     3421 ggatttaacc tcacttactg gttgttcata attactaggc ttgatacttt catcaggaac
     3481 aaccactgcc acttgcgaaa cgctttcgga cttgttccct tcccagaatc ttaactcaga
     3541 aataaagata aatgctgcaa caacgcctat aaccataaca gcagcaaata aacctgctgc
     3601 aaacgaccgc tgggactttt tgggagttcc aggaatatat gatggggttg cttcaacatt
     3661 aactgtagga gtggatttat cttccttgcg ataagttggc atggatgaac ctaaatcctg
     3721 attagcttca accttttcag ttccttgatg gttaacaaca atatcaactg gtggtaggaa
     3781 tgttgttgct aaagcatcat cggcaatcag ttcaattcct ccatcaaccc cagttttgag
     3841 cgaacctatt ccgggaatag aaactgtttt attctgtttt aagctattca aaagagtttg
     3901 acaaaactct tcaactcttc tgcttgcctc actttttgaa aggccataac ttgcttcaag
     3961 gtaattctca agtgcctcat cgttaaatgt tcggttaggg tcaaaaacaa agtactcctt
     4021 aggaggcaga agcgttttgc catcgttggt tagctgagcg gattcgtact tccgaacaat
     4081 gctgccaagc cctggaaggc taacataatc gttggtttgc aaaatttttt gcactatagc
     4141 attcaattcc aatgctgtag ggtgtttggg gtttaactaa caaatgtata aaattatttg
     4201 ggttaaatca aaatggttgg gttatggttt tcaaaaaaat acattcaact tttttttaaa
     4261 taaagatcaa tgtcgtttag ttaacaaaat atacatacct tttgtcatcc tgagtgaagc
     4321 gaaggatctc taatcgatcc catgagatgt ttcgcttagc tcaacatgac aatagtttca
     4381 cgaacaacgt ttccagcccc gtaggggcta aacatttgta acaccatggc tactcccaca
     4441 cgtatagcga tgcacgcaat acattttcca aagagcaagg ggaaaacgtt gcatcgctga
     4501 agaaaagcat tacaaagagc tatttaaacg ttctttcttg tcttgataca agaaagaacc
     4561 aaagaaaatc aaggctgaaa agcctaaccc gatgggtacc ccgtgggtgg aactgccacg
     4621 cgatacaatt cgccacgcct tcggcgtgac tcatgtggta tcgcttacaa aacccaccgc
     4681 cacgttccac ccccgaccca ttgccgggtc tgacttttat gccattgcta aaatcattcc
     4741 tttggggttg aggaatttca ctaacagaag gaatgaaaat acaaagagat gcttcgacaa
     4801 agtctatccc gaaatatcgg gactcagcat gacagcgtaa tgtttcgctt gcgcgcaaca
     4861 tgactaataa ccttaactaa acaacactga aattaagata gaaatagagg ttgcccatta
     4921 ggacaacctc tattacatag actatctacc cttcctaaaa cttgtaccag aaaccaattc
     4981 ccatagagta tgatggtccg ttttcatatt tctgattaaa aggagcaacc cattcggttg
     5041 attcaacttt acgctcttgt gtagccaaac ctacctggaa ccctaagtcg atacctaagt
     5101 tttcctttag ctttaagcta gcgccagtgt tgagccaaat ggtgttattc tcaaattcca
     5161 tttccgattg atattgcctt ttcatcttat agggagcata agtacagcca acaccccagt
     5221 tgaacttttc ggtaaactgg tactcagccg ctaccccaaa atcgatacca ttatggtagt
     5281 attcggcaac gttaactcca tcaacatcct catcgagtga gagcatggag tatagtgaaa
     5341 cattcatgtt atagtgcaag gttaactttt cgttagcatt ataggccacg ccaaggttat
     5401 acgttgggac ataggttgat ttcaccttat cgccgtcggt aaataatcca ccacttttgt
     5461 tatcattaac cttggtttca agttccattt cagtgaagta gcggaaagta tttgctatta
     5521 gtagatttgg cataggtttt aggtcaacac ttccaataaa accaaaactt tgacctgtaa
     5581 aatctacatc tatttcagtc cttggaatag gtacagcaga tggtgcaggg gtataggtga
     5641 tatccaacca accctcctga tggttcatct ggcgggaata ctgaaaagcg ccaccaaccg
     5701 ataaccaatc gttaacacga taggctgcac ctagccaacc ccctacacca taagcagaac
     5761 ctttaaaact agttttaaca ccataatcgg ttactgcgcc aggcagcaat ccacccatta
     5821 taccagtaat aacattgaaa taacccaaac gctcaaatgt tggtaatcca tcgttataat
     5881 tgccggcgcc accaccattg gtgattcctg caaacataaa tagcgcaaac ctatcctttt
     5941 tataggtaag atttagtgct ggataaagaa gcgaactggt tttactcttg tactctaaaa
     6001 tattgccgat aaaattacca tcatcgaaaa tgctcttggt ggagtagaaa ggaattgtag
     6061 agaactcgag ggcaaacccg tcgtttcccc aaacggttcc tgctgggttg taaaaggggg
     6121 catcggttgt ggttgatgca tttcggtttc cggtcttcat tttggaagca ctaccattat
     6181 tggcaggatt gtaatcggcc attgccacca tggttccaag cataaaaatg ctaagcaata
     6241 aaacgtttct tttcatatca gtttggttta aggtttgatt ttcaaaaaat tcagctcaac
     6301 atgtcgggca ggtagaaatt tcacccctaa cccaattgtt aatgagcgtt aacaaaagtt
     6361 gtttttaaat ggtggttatt cgttcaaata aaactttgaa cctccgaaat gttgttcttt
     6421 taaattatga acaatgcatt gaacagctca tttgggcagg aaaactcaat gtagccaatg
     6481 gtatgtttag gactagctaa tgaccattgc aaattaccgt gtttgccaga aactatgaat
     6541 atctttcttt gcatggttag ttaagtattt cgcttcgctc aatttgacaa gtaaccgtat
     6601 ttaagtttga ttgaaacaca tgaaggcaaa aggttccttt tcatcaatag gagtacgtta
     6661 ccatttaact cgtgaacata gctttacagc aatcaataat tgttttagga aatggtaaac
     6721 caatattgct aagctgaaat ggttaataac aaaaaggccg ccaaaagtgg cggccctgat
     6781 aaccatgcat gttcagattt actttactgt tgaaccattt gataccgaat cggccggtat
     6841 aaccagctgt aaccttccat ctacatcctc ggccataaga atcattccat gcgactcaat
     6901 tcctctgatg gttcttggct caaggtttgc cagaactact acctgttttc caaccatttg
     6961 ctcgggcgta aagaactcag ctatgcccga aacaatagta cgggtatcga ctccggtatc
     7021 aattttaagc tttaacaact tttgtgtttt tggtaccctc tcagcctcaa gaaccttagc
     7081 aatgcgaatg tccattttgg aaaactcatc gtaggtaact gaatctttca agggggttac
     7141 cttgatattt gccctctcgt tagcctcttt agctttccgt aacttttcga gctgccgctc
     7201 aataacgtcg tcttcaatct tatcgaacag aagcgatggc tcgttcagct ggtgaccggg
     7261 caacaaaaga ttatcgccac ccgccagatt ccaatccttt ccatttaggt ttaccattgc
     7321 tctaatcttt tcagcggtaa atggaaggaa aggctctaat agtactccaa ggttagcagt
     7381 aatctgcatg gaaatgttca gtatggttgc aaccctttgt gggtttgttt tgtaaacctt
     7441 ccagggttca gtgtcggcaa gatacttatt accaagacgg gcaaggttca ttgcctcctt
     7501 caatgcttcg cggaagcgga attcatcaag gttttgctct atggattgtt taagcttggg
     7561 tatctctgca agggtttcgc ggtcgtaatt gtcaagttcg gcaattgcag gcacttttga
     7621 atcaaaatac ttctgtgtaa gaaccattgc tcggtttaca aagttaccca gaattgcaac
     7681 caattcactg ttattacggg tttgaaaatc cttccaggtg aaatcgttgt ccttggtttc
     7741 gggcatgttg gccgtaagaa cgtacctgag cacatcctgt ttaccgggaa actcatcgag
     7801 gtactcatgg agccatacag cccagttacg cgaggtagat attttatcgc cctcaaggtt
     7861 aaggaactca ttggcaggaa cattatcggg tagtatatag ctaccctctg ccttaagcat
     7921 agcagggaaa atgatgcaat ggaaaacaat attatccttt ccgataaaat ggatcagcct
     7981 ggtttcaggg tccttccagt acttttccca gtttggcgtt aactccttgg ttgcagaaat
     8041 atatccaatt ggagcatcga accatacata tagcaccttt ccggtagccc cttcaacggg
     8101 aacaggaaca ccccaatcta aatcgcggct aacggcacgt ggctgtaagc cttgatcgag
     8161 ccaactcttg cactgtccgt aaacatttgg tttccattcc ttatgctctt ccagaatcca
     8221 tttttccaaa tcgggttgga aacggtctag tggtaagaac cagtgcttgg tttccttcat
     8281 cacaggcttt gacccgctga gcatggatcg gggatccttt aggtcggttg ggctaaggct
     8341 tgtgccacac ttttcgcact ggtcgccgta ggcgcggtcg tttccgcaat gtgggcaagt
     8401 tcccatgata tacctatcgg ccaggaattg acctacctcc tcatcgtagt actgttcagt
     8461 tgatttctca atgaatacac ccttatcgta tagcttccgg aaaaattcac tggcagtgtc
     8521 atagtgcacc tttgaggttg ttcgggagta aatgtcgaag gcaatgccaa acctctcaaa
     8581 ggaatccttg atgattgcgt tatacttatc aacaacagct tggggtgtgg tgttttcctg
     8641 acgtgcctta atagtaatag gaaccccatg ttcatcggat ccgcacaccg agattacctc
     8701 aactccttta gctcttaggt agcgggtaaa aatatcggat ggtatgtaaa cgccggcaag
     8761 gtgacctata tgaacaggtc catttgcata gggcaaagcc gatgtaatca ggtaacgctt
     8821 aaatgagttc atctgtcgtg tattaaataa cttagtattt caatggtgtt tactaaaaaa
     8881 gctgtaggtg tgactttgta ggcaactaat gcatatgtat ccatacccag ctattttagt
     8941 gccacaaatc taattgattt tctttaaaga accgaaaagt atttcaaggt gaataagtaa
     9001 aaccttaatg acaaaaatga gatttatttc aaaaaataat ctaactttaa agtccatttt
     9061 tttacaacag gtgcatggaa aacataacac ccccatacct acaaccaggc gatacaatag
     9121 ggattgttgc tccagctcga tcaataaccc gcgatgagct acagccggcc attaggtttt
     9181 taacatcaca gggtttttta gttaaaacag caccacacct atactctaaa cactaccagt
     9241 ttgccggcac cgatggcgag agagctcagg atttaatgga gatgattgaa gatcaatccg
     9301 tgaaagctat tctttgtgct cgcggaggat acggctcggt aagaatattg gatttattaa
     9361 acttgcggaa gcttcaacat aacccaaaat ggattattgg ctatagcgac atcaccttct
     9421 tgcacagctt aattaccggt tggtttggca ttgaaacgct ccatgccacc atgcccatca
     9481 atttccccga ttctgttcaa ggaaaccaat cgaccatggc gctgattagc gctttaaaag
     9541 gtgagacctt atactacgaa ctaaaacacc atcccttaaa ccttcctgga catgccactg
     9601 gggttctcat tggcggaaac ctttccatcc tttcatcgct cagtggcaca acaagcgaca
     9661 tgtcctatag cggtagaatt ctattcattg aagatgttga tgaatacctc taccatattg
     9721 accgcatgat tatgcagcta aagcgttcag gcaagcttaa atcaattgcc gggcttattg
     9781 taggacactt cagcgacatg aaggataata gcgaaccttt tggttccgat gcttacgaaa
     9841 ttatttacag cgctgttaag gaataccgaa tacctgtttg ttttggattt cctgctggac
     9901 acgcagagct taacctccct ctaataattg gtagaaccgt tgaattaaag gttagcgatg
     9961 agggggcaaa agtaggattt tacaggagtt taacttgata ttaactcatt aaacccaact
    10021 ggaatagtaa acagggtttg cgaaatctgc atgggcgtaa tacttaaaac ccttatgttg
    10081 cttcgcagct tccggaaccg ggtacgatca actaccatca atggcaatat cccttcgggt
    10141 tgtggtaaac ttaccaattc ttcaatagct gatccagcat tggccaatgc actgaaaagt
    10201 ttaggagcaa agtcgtactt tgtttccgat acccaatatg ttgtttcagt ttgagtggct
    10261 aagtttttta ccctccactg gtaacacctg ataccattaa tttcaatata gttctctgac
    10321 tttagaatac taacatttga aattgcggga gaattatcta ccttaacttt ggagtacatc
    10381 ctattgctga atgataccag ataggttgat ccactttcca cattggcaaa gtaggatcgt
    10441 atcaacttac cacctttact aaactcgtta acacgtatta ggttgccctt aatgtaaacc
    10501 tcaacctgta tggtatcgta tacaccctca cgaacaatat tgactttacc ctcaaaaccg
    10561 cctacctttg agtaggcgtt tgtggatagc agtaaagtga agaatgctat gtagcaaata
    10621 aaccgcaagc ccaaaaaaag agttgatcaa caaaaattta tatcgatttt tgttaaaaat
    10681 tgtttcatag aatggtagaa aaaattggtg gcaacaagga tttaggcagt aagggcgaag
    10741 aaattgctgc aagttacttg gctgcaaagg gatataaaat tttacacagg aactggcact
    10801 ttggccacaa ggaactcgat ttggtgacac aactaaacga cacccttgtt atagtggagg
    10861 ttaaaacccg aagtacccac tactgggaag agcccaagga atcggttaaa cggaaaaagc
    10921 aaagaatgat tgttgaagct gccgacgagt atgttcaaag acataacctt gattgcgatg
    10981 tacgattcga tattgtttca attgtaatca accatggctt accacctgag attgagcata
    11041 ttgaagatgc atttttccca acactataat cttcagcaat tacagaccta tgaatattga
    11101 actgattaga gagcattgca tatcaaaacc agatgtaact gaaagttttc cgttcgacga
    11161 cgacaccctg gttttcaagg tgaacgggaa aatttttgcc atggttaacc ttagtgggga
    11221 cacacagctt aaccttaaat gcgatcctga actcgccatg gagctgcgtg aaaaatacat
    11281 ggctgttcaa cccggctatc acatgaataa acgccactgg aacactatag caatagatgg
    11341 gtcaataccc gatgccttga ttttggattg gattgaccat tcctataatt gtgtttgctt
    11401 aaacaagggg aaacgtaaat taggtagtag aaagaagaag tgattcaaga ataaatttcc
    11461 tatatacgtt agttaaggat atatcatgtt gagcgttagg gaaaaagccc tttgtcatgc
    11521 tgagcccagt cgaaacatct cattgaacct acaactcttt attaattgag attcctcgac
    11581 tagctcggaa tgacaatatc cctaacaccc tttgtcatgc tgagctcgcc gaagcatacc
    11641 ttttgtatct tctactcgtt attaatgaga atcttaaaac aacagggata gccataagca
    11701 atggcataaa agcctggccc gacaatgggt cgggggtgga acgtggcggt gggtctagta
    11761 agcgatacca catgagtcac gccgcaggcg tggcgaattg tatcgcgtgg cagttccacc
    11821 cacggggcac ccatcgggtt gggcttttca gccttgatct tctttggttc tttcttgtat
    11881 caagacaaga aagaacatgt gaatcgctct ttgcaaaacc tttccgttgc gatgcaacgt
    11941 ttcagtgttg ctctttggaa tatgtattgc gtgcatcgct atacatttag cattagccat
    12001 agagttacaa atgtttcgcc cctacggggc tagaaacggc gttcgttgct cgtgtttcgt
    12061 tgttcgtaaa tatcacgtca cacgatctgt gtatcaccgt ataacattgt gtaactctat
    12121 gatacttgct gttgctcgtt tcaggattcg gttgtcatgc tgagcccagc cgaagcatct
    12181 cattgaacct acaactcttt attaattgag attcctcgac tagctcggaa tgacaaaccg
    12241 ctggcatcca cacgcgatga agcgttgcaa taaaaccaac tctaactttt tctattcctc
    12301 atggaggata gactaagcca gggttaaagt aatttttaaa attttagttg gatagtagtg
    12361 atatttaatg gaaaaacggt aagggttaat ttgaaaagtg taactttggt atattatttg
    12421 attatgtaat taaaaaaatt tagccatgaa accagtagtt caccttttaa cagtcctttt
    12481 gcttgccata tcggttagtg tgcaggcaca ggaatcgttt attgttacaa gaataaacgg
    12541 tgtggtaacc aacaacaaaa caggaactca gcttaaagct ggcgatgtgc ttcaaccatc
    12601 cgacaaggta acgttcgact cgtttgactc ctacgtaatt tccattggac aaaacatggg
    12661 gcgttacatg attaagctgc atgagccacc agctcctgaa accattcaac agctatcggc
    12721 catggttaag gatattgctg ttcccaccaa gcgcagaagc cttatggctg agcgttaccg
    12781 ccccgatgaa accaccgttt ccgacctccg ttcctacttt ggcaatgaca agttcaccat
    12841 tattggcgat aatattgctg ttccaattaa cccccaatcc taccccattg atgaaaataa
    12901 gttcgtggta ttctactacc gggtgaataa caatccggtt tcaaaaaaga ttgggtatga
    12961 aaacacaacg cttttcattg aacgcgataa gcttgttaac acaaatgccg gcgtaataac
    13021 ccaaaacgag ataccttcgg ttgcagttta cctttacgaa aggaacacaa aaaccagcga
    13081 ggagattaca aagtttaatc tggcctttgt tgacaaggaa actgtaacca atgagtttat
    13141 gaccattatt ccaatccttg ttaaacagaa aatgggtaag gatgaaatca aaaaatactt
    13201 aatagaatac ttctacgact tttatggtgc taccgattcg cgttccatta aagcctttgt
    13261 tgatgaactg gttgatacgg ctattaaata aacactcgca aagtaaagta aaggggtgct
    13321 ttctaagcac cccttttttt attggttaaa tcaaaagtaa tatatttaag aagatttact
    13381 ttttaataat ctccatggtg tagagcaaag cctcaacctg tcggaggtaa ttacgtttct
    13441 cctttgatgg agcgtaaacg tatgcctcag caacaataac cctttggcgt tcctcatcaa
    13501 cagtaatatg gctgatgaat ggtccaccca ttggatgttt atatacatcc cacaaaccac
    13561 gcataactcc gtaataacga tttttgaaca tcatatcggt aagctgcgga acaataacgc
    13621 tactggtagt catatacgaa ccctgagttg gaccactaat ggttttaaca aactcattgc
    13681 gcttagccaa aaggtagtca accgtaaagg tgttttttgc tgtatagggg aaagtgtaaa
    13741 gaataatgcc ctggcttgta agcggagttt cataggatac ccaagcaata gtgtcgtggt
    13801 caaagttcag cctgtagcct ttagggaaat acatcgaaac accatacttc ttttcaagtg
    13861 cttccctaat gggcttttcc tcataggtaa tggcattctg tatcacccta tcgcgttcag
    13921 cctgctcaaa aatgcttaca actttatcgc gctcctttag taggaggtcg taaattgatt
    13981 tatagctagg ccccgagatg taaagaatgg tttgaggtgc agcccaaaca tctcttttaa
    14041 gagacatttt tgcctccttg gcacccttaa ctatatcgtt aaaaatgatg ttacgatgtg
    14101 gcttaaagat atcggtaaac gcttcgggtg ggatcatcac catatcgaac ctaggttcgc
    14161 tttgaggcaa aaagggaaca tcctcagtta aaatatcgct atacagctta cccagtgaat
    14221 cgctatgaag tgcttttggc agtattacca caagctcatt agcacttccg gttacattgg
    14281 gtaaaagcga ccgctcaccc ttgctcccct ttttgcatga tgggctaagt gcaactgcag
    14341 ccgcaagtat tattatccac ctacttttca taatattagt tttgattttc aagataaacg
    14401 cctataaaca atatttattg ctaacgccta aaacctttca gagattttta gggttaaccc
    14461 aaataacaat gttttggcct gcagttattg tgctatccaa tccgggattc cacctgtaaa
    14521 tatcctggag ggtgcaacca aactgcattg ctaacttatg caggctatcg ccattcttaa
    14581 cggtgtaaac cttacgggtt aaagtgctat cggcagctaa cgcaggcaaa atcttaggtt
    14641 ttgatgtgtt caggtaaagc tcctttgatt tcctgataaa taatccagtt ttatcagcag
    14701 gcaaccgtaa cacctgtggg gaagaaccct cagggatata atcgtaacga taggttgggt
    14761 ttagaaagcg aataatattg atatcaatat caatggcctt agccataagg ttaagatcga
    14821 ggggtttatt tacccaaact gtatcggttt ggttttgagc taaagctaca ggagcaggcg
    14881 taatgccatg ttcatggtag ttattcatta cataagcagc ggcaataaat gccggcacat
    14941 agttttgcgc cgattcaggg aggagtggat aaagcttcca aaaattcctt tcgccgttag
    15001 ctcgctcaat ggcattccta atgggcccag gtccaacgtt ataggctgcc agtgctaaac
    15061 tccagtcgtt aaaagttctg tagaggtatt gcaggtactg agcggctgcc tcagtagatt
    15121 tcaaggggtc cattcgctca tcaacaaagt tgttgacctg aaggttaaac atcttggatg
    15181 tattaatttt aaactgccat aaccctactg ctccggttgg ggatattgcc aatgggttta
    15241 atgccgattc aactactgca aggtacttta actccaacgg caactggtat ttgctgaaca
    15301 catcatcgaa aagggggaag tagtaattgg ctaagccaat cattttagaa atttgctcac
    15361 gacgctcaat ggtaaaaatt tcgatatatc gccttacatg gggattgtaa tcaaaatcaa
    15421 tgggcgagtt ccggccaatc tccgcaagct ggcactcaaa gtaaaggtca ggtttttgcc
    15481 tagctgtgtc ggtttgattt gtggggacga aaatgggaag attttcaata gcatcaatga
    15541 aagacactat agggttttgt cccctattta atttaacagg gtttgaaaac ccgtttaaaa
    15601 agacaagtga gaaaagtgat aaaagacacc ccctaaatcc taaaaacata aatcattcct
    15661 tttctttatc ctcttgattc tcggcttctt ttataccttt tttaaactca cctacgcctc
    15721 tacccaggtt acgcatgaaa atgggtattt tgcttgcgcc aaaaattacc aggataacta
    15781 tggcaataac aagcagctct ccacccccaa taaatagtgt tagcatacag aactctttta
    15841 cctaaaaagt ttaacaatat cgttgatgtt ggcatacaaa agcaaggcta aaaggattgc
    15901 catgccaaca agttgagcgt attcaaggaa cttatcgccg ggtttccgcc cagtaataat
    15961 ctcgtaaagc aagaacatta catggccacc atcgagcgca ggtattggaa gaatgttcat
    16021 tatggcgagg ataatggata ggaatgcagt taagctccag aatgcctgcc aatcccaagt
    16081 tgaggggaaa attttaccaa ttgtaataaa cccgccaacc gattcgtatg ctttggtttt
    16141 gggcgagaaa attagcttaa gctgttttaa gtagctcgat atggttgcaa aacccttttt
    16201 aacaccggca ggtattgagt gaaagaacga atattccctg tggctgagtt caaagaactt
    16261 tcctaaatcg gcaacagggt aaacccctat aatgccagtt tcgggtacat caacatttag
    16321 gtcgataggg tttccgttgc gctcaacggt tacccttaca gttttcccct tactacgctg
    16381 catttcggat ttgaactcat caaaaaactg tgcagattgt ccgtttaagg caagcagcct
    16441 atcgcctttg ataaaccctg ccttttcagc aggttctcct gacttaacat catctactac
    16501 aaatgggatg cgcgggaaaa aaataattgg gcttttaaca agtttagcca cataatcctc
    16561 agctatttta atgttcaatg tatcggtgcc acgtagaacg gtaacgtcac gattctcatt
    16621 tagcagaata tcatgagcaa tttgctgaaa gttttcaatc tccttaccac ctacataaag
    16681 tattttatcg ccattcctaa aacctaactc tgtggctaag gaatcgcagg aaacaccgta
    16741 tttaagactt ttcgtaggaa ggtaatcttc gccccatgtg tatagaactg cgctataaat
    16801 gaacagggcc agaataacat tgacaagcac tccgccaatc attattagta agcgttgcca
    16861 tgcaggttta gagcggaact catagggctg tgggggttgc ttgagctgtt ccttatccat
    16921 ggactcgtca atcatccctg aaattttcac ataaccaccc agtggtaacc aacccactcc
    16981 gtactcggtt tcaccacgct tgattttaaa aagcgagaac cagggattga agaaaaggta
    17041 aaacttttca acacgggttt taaaaagctt tgcaaaaaga aagtgcccta gctcatgaag
    17101 aataaccagg attgaaaggc ttaaaaagaa ttgaccagct tttatgagta tctccatttt
    17161 atgatgtttt tattaatgag ttcaactgat tttattcgag tttcggaatc ggtttgaatg
    17221 taatcggcaa aggtaggttg cccaataaac ggtatggctt gcatagcacc ttcaacaacc
    17281 tttgggattg ccataaagct aatctgctcc ctcagaaaag catcaacagc tacctcgttg
    17341 gcagcgttaa gcacgcaggg catattaccc cctgccctta acgattcgag agccagggca
    17401 aggcttggaa acctattggt atcgggtttt tcaaaggtaa gggtgtgact catggaaaaa
    17461 tccatgcgcg ggaaacctga gcttactctc tcagggaaag tgaatgcgta ctgaatgggt
    17521 agtttcatat cgggtaagcc catttgggct tttatagacc catcaatgaa ctgtaccatt
    17581 gaatggacaa ttgattgcgg atgaataacc acctcaattt gctccgggtt aaggccgaaa
    17641 agccacttgg cctcaataac ctcaagtccc ttgttcatca ggctggctga atcgatagtt
    17701 attttttgcc ccatacacca gttagggtgg ttaagcgcat cggccttgga aacatgcatg
    17761 agttgatcca gggaatggtt gcggaatgga ccacccgatg cagtaagtat tattttttca
    17821 atggggctgc gctcccctac caggcattga aatatggcgg agtgttcgga atcgaccggg
    17881 attattggta cccttttttg catggccgct ttagtaacca gctcgccggc aactaccagg
    17941 gtctccttat ttgccaatgc aatggcctta cctgcctcaa tggcgctaag cgtgggttca
    18001 agcccggcaa atccaaccat ggctgtaaga accatatcga cctcggacga tacgaccgat
    18061 tccactattg actgtttccc cgtatatacc ttaatgggat gatttgctag ggcttctttt
    18121 actctttgat aatgactctg atttgcaata attacggtgt ttggctgaaa ttcaatggct
    18181 tgctgcacta agagctccca gctgttttgg gctacgagta gctcaacact aaatatatcg
    18241 ggatgctccc taataacatc gagcgcttgc gtaccaattg aaccggtaga acctagtatt
    18301 gcaattcttt tcataccggc cactaaaatt taatgtactt agcgggatca acggtggctc
    18361 cgtggtgcca aagttcaaaa tgtaggtggg gtccagtggt aagttcgcct gaattaccaa
    18421 ttattgctat cacatcgcct gaattaacaa attcaccctg cctttttaac agcttactgt
    18481 tatgcttata taccgaaagg aatccctcct cgtgctgaat ggcaattgtg taacccgttt
    18541 ccagcgacca ggttgagagt attacggttc cctttgcaat ggcaagtacc ccctcgtttg
    18601 gcccggcaac cagatcgact ccgtaatgat tttttgaagg gttatacttg ttagtgataa
    18661 tacccttaac gggtggaata aaaaagggta tattactttt cttggttgta cttgatccta
    18721 ccgacaggtt aaacatctca tccgattcaa tttgcatacg gagcaatgaa tcctcctctg
    18781 aaggggatag ctcaaccttt gcggtaggct ttaccgtatc gattactgtt tggcgaccag
    18841 taaggggttc tcctccgtta agcaggatat tgatatcctc atagtaacgc tgccattggg
    18901 ctaccaccct ctcaagcgaa tcgaccttca gggcattttt aacataacct ttccgggttt
    18961 ggctatcggg gtaaccggga atgaactccc ttaggggtgt aaaggcaatt aggacactca
    19021 caagcgcaat aagcagaata gaaaatccgc caaagaaaac tattacgttc attctggaaa
    19081 gacgaagttg ccaaacctct tcatagttct cctcattgaa gatactcaac ctgtacttat
    19141 tccacaggtt cttaaatatt ttaacctttt tcttagctgc cacaggtaca agggttagat
    19201 tctaaaatca caaagataac aaaatgagca attagttgtt tggtgtttct ttttgatgat
    19261 aaaggtcaga atattatgaa gcaatcttca tcggtaaaca aagcaatgcg taataggaat
    19321 atcaacttac ctatttaaag aaaatcaaaa aaaccaatgt aaaacctgta agaaacccag
    19381 tgtaaacttg agttgggtta tgatcgcctt taataagacg ggccgaggct aaaagacctg
    19441 aaagtaaaat actcactata aaaattggta ctgcagcctg gttcccttgc agagccgaag
    19501 ccaaaagaaa cccagagata cctcctattc cagttaaatg aaggcttatc ttccaaaagt
    19561 aagaaatggc tgccgagatc atcagtgtat aaattcctgc ctgaagtatt ttgattaaaa
    19621 caaatggaac ctgaagcctg gaaaaaagaa agatggtgaa aaggtaaggt aaaaccgata
    19681 tggctagggg gaaaacccta tccctactgg cttttagagt ataatcgtga ataattctaa
    19741 gacgatgaag gagaacaatt aatagtaatg gcagaaaaat agtatttata gctacaaaca
    19801 ccgtaagcat caccttaaac cttacaggta gaaaggcaac aaaggagttg gacgagaaaa
    19861 tcactataaa aatataggtg gtagccagta tagggtgaag aataattgaa agtataagcg
    19921 aaagtcgttg tagcatttta aaaaaataga ttagttaagt tcttttctta ggcgagcaac
    19981 cggaattcca agctgttcac ggtacttggc aacggtccga cgggcaatag ggtatccctt
    20041 cccttttagt atttccatta gctcctcatc ggtaagggga ttgcgcttat cctcattatc
    20101 gacacactcg gcaagtattt ttttaatttc acgggttgag acctcttcgc cggaatcggt
    20161 ttgcatgccc tcgctaaaga agtgcttgag agggaatatc ccgaaatggg tttgaatgta
    20221 cttgctgttt gcaacgcgcg atatggttga aatgtcgagc cctgtttttt cagcaacatc
    20281 cttcagtatc atgggcttaa gcttactttc gtcgccatcg cgaaaatact ctttctggta
    20341 atcgagtatg gcctgcatgg ttgtcagcag ggtattctgg cgctgtttca gtgcatcaat
    20401 aaaccacttg gccgattcaa tcttttgctt tacaaaagcg atggcctcgc gttcctggct
    20461 attgctaaac tccttgttgt aagaatacga ctcgagcata tcggcatatt ctttgcttat
    20521 tcgtagctcc ggcacgttgc gcgagtttag gtttaaccta aactcaccat cctcgtaatc
    20581 gagaataaaa tcggggatga tatgctgtgc cgattggtcg taagggtcag taaaactgcc
    20641 gccgggtttg ggatttagct ttaaaatttc atcaattgcc agcttaagct tttcgtcgga
    20701 tagattaagc tttgaagcaa tcttatcgta atgtttcttg atgaactcgt caaagtggta
    20761 cttcagtatt ttataggcaa ttttagttat ctcgttttgc tgctccttgg ccttaatctg
    20821 aataagtaaa cattcctgga ggtcgcgtgc acccacaccg ggtgggtcaa attcttgaat
    20881 tacgtaaagt attcgttcca gctcatcctc attggtttca atacctaacg agaaggcaat
    20941 atcatcggca attgcggtta acttacgacg tatataaccg tcctcatcga tattgccaat
    21001 taggtaaaac ccaagctgct tttcgcgttc ggtgaggtcg gccattccga gctggttttc
    21061 aagatgctcg tgaaacgaga ttccggtgga aaaagggatt tcctcccgct tatcgtcggg
    21121 cgatgcgttg ctggagtata gcttgtacga aggaatatcg tcatcgttca tgtaatcctc
    21181 aatggagaac tcatcgtcgt taccctcctg gttatcgtcg ggtaaaacaa tttcaggttc
    21241 cgtttgaggt tccgtttgtt caacctcctc gagaatggga ttttcctcca gttccttttt
    21301 aatacgctgc tcaagcaaca tagtaggtat ctccagcagc tttatcatct gaatttgctg
    21361 cggcgatagc ttctgaagaa gtttttgttg taaccgttgc ttgagcatag acttttagaa
    21421 ttcgcagttc ttgggagttc tggggaaggg tattacatcg cgaatattgg tcattcctgt
    21481 tacaaagagc agcaagcgct caaagccaag cccaaaacca ctatggggag ctgagccaaa
    21541 cttgcgggtt tcaaggtacc accaaacatc cttttcagga atatgaagct catggatacg
    21601 gcttagtaac ttatcgtaaa cctcctcacg ctgggaaccg ccaattatct cgccaatttt
    21661 tgggaacaac acatccatgg cacgaacggt tttgccatcg tcgttttgct tcatgtaaaa
    21721 ggcctttatc tccttggggt aattggtaag aataacgggt tttttgaagt gcttctcaac
    21781 caggtaacgt tcatgctccg actgcaaatc ggcaccccag aatacaggga attcccactt
    21841 atggttagct gcctccagta ttttaatagc ctccgtgtac gttaaccttt caaatgggtt
    21901 ttcaatcaca aacttaagcc tgtcgataag ctcgttgtcg tacatcttgt tgaggaattc
    21961 cagatcgtcg gcacaatgct caagggcata tctaattagg tattttagga aatcctcggc
    22021 caaatccatg ttatcctgga tgtcgtagaa agccatttca ggctcaatca tccagaactc
    22081 agccaggtgt cggggagtat ttgaattttc ggcacggaag gttggtccaa aagtgtaaac
    22141 ctccgatagg gctaaagcgc ccagttcggc ctcaagctgt cccgaaacgg ttagctttgc
    22201 ctccttgcca aaaaaatcct cgttccagtc aatctttcca tcctcggtgc ggggtagatt
    22261 ctccaaatcg agggtggtta cgcggaacat agcccctgcc ccttcggcat cggaaccggt
    22321 aatgatgggt gcgtgaatgt aaaagaaacc cttgtcgtta aagtacttgt gaatggcata
    22381 cgccatggca tgccggattc ggagaactgc tccaaaagta tttgtgcgag ggcggaggtg
    22441 cgcaatctcg cgcagaaact ccatactatg gccttttttc tggagcggat aggtagtggc
    22501 atcggcttca cctaatacct caaggtagtt agcctgaatc tccacgcgct gaccggaacc
    22561 gggcgactca accagcaatc ccctcaccgc aatacaagcc ccagtggtaa ctcccttaag
    22621 ctgttcttcg gtaaaacggg tcatatcaaa tacaacctgg atgttatgga tggttgagcc
    22681 atcgtttaac gcaacaaagg ctacattctt attcccacgc ttggtacgta cccatcccat
    22741 tgccgctacc tcagcgccaa caactccact ctcgagtagt agttttactt ttgttctttc
    22801 actgttcctc atgcctgtat gatatcttac tcaaaaacaa tacttttata cactgtttta
    22861 accacacaaa aatagtaact tttatcagat ttcatgaatc ttagtagttt tcgtttgtat
    22921 gaatgaaaag ttttttgagc gctggggggc gacatttgtt gctacactcc tattactaat
    22981 atacatactt ggaatatcaa acctgagtac aattgatggt tggggatatg ctgctgacat
    23041 tgtaaatagc agtaacctgc ttcgtccaca tcacctgcta tacaccatta caggatttta
    23101 ctgggttaaa atatggcact tttttctgcc aaatgccgaa accatttacc tgctcaagct
    23161 gatgaatgcc ctttgcgcat cggttaccgc tttcattcta ttcaagctac tccgacttgt
    23221 taaggttgat accattcaaa ccacaatcta taccattatt gcgggactaa gctggggatt
    23281 ccttcggttt tcaattgaca atgaaacata cactattcca gtgatgctgg caatttgggc
    23341 tagttacctt ttccttaaag ctgagagtag ccccaaaatc tcaaacatgg tttattcagg
    23401 actactatcg gcatcggcgt gtctttacca ccaaataatg tttttttggt ggattgccct
    23461 tgcaattgga accataagga aattaaatcg cagggattac aaacccctgc ttttccttgc
    23521 accagcagta atagtgcctg ccgcttacat ccttgccatg ctatcaatgg gtatcgaaat
    23581 caattttact agctttttcc ggttcatatt tagcgactat tacactggaa acgcatcaat
    23641 tgaattgaac tccacgattc ttatggttgg cataatcaac ctattcagaa catttttcca
    23701 aatgcatggg tatatcaact atcttcttgc acaatcgtgg ctgtggttga ttcctttagt
    23761 agtatcgggt attgcatttg catttttttc tttttggctt gttaggtata caaaacctac
    23821 cagacttgaa aaatttcata aaattcactt gctggcttta gtattacagg taggttttgc
    23881 ctttatggcg ggcggaaacg ctgagtttat ggccatggtt cctttcctaa caattatcac
    23941 tgcaaccggt ttgtcaataa aacgggatgc cactgtttgg tttgccatct cactttcagt
    24001 atggaacatc tcctttggtg ttcttccaca aaagatttac caggccgatg gttccgattt
    24061 ggttataaag tcgattaccc aaaataccaa tgaaaggaga gcctatatcc ttaccgatgc
    24121 tccaaaagtt atcaataggc ttgaatatct gcaaattcaa aaacctttaa tcgttaagga
    24181 ggttaacatt gagccgcaaa aaatttatgc cacactcgat agcctactat tgcaggggta
    24241 caccatttac actgactgta taaaccagcc acgtgttacc tcaagggcaa cgttaattac
    24301 agatgttagc ctagaatggg ataaactaga gggttacaaa acaacctcta tagattcgtt
    24361 ggaaatgctt ggcggtaaag tttatgtcac taaaatatca aggtaagcct agtagccgct
    24421 accgtaagtt ttaaagctgc ccatgataaa tatctctttt ggggtaacgc taagaacagg
    24481 gattgggctt ctactcagca tctgttgggc ttggctgcca agaacgaaat cgctaagggc
    24541 aatgttttgc tcggtcataa tactaataag gtcggcattc acatcaaggg catactcaat
    24601 ggtaatatcg gtgatattat caccacgtag gcttgccttt tggaaacgca cctcgtgctc
    24661 ttttaggtag ctgcaaacct gcttggtata ggcattgagt ttgaggtcaa tttcatcggt
    24721 ttgcatggta gccactgcaa ttacatgcac ctcggcatca aaaaccttag caatttcagt
    24781 ggtaatggga actttctgac gagtatcggc agtaacatcg ataggcaaga taattttttg
    24841 gatatcgcgc ggcatggcac catgccttat ggttattacc ggacattgcg tagccgaaat
    24901 aatcctaaaa gcattgctac caataaaaaa ctcctcaaaa cctgaggctc catgagtaga
    24961 gcaaataatg gctgaatcct caaaagcatg cgcctggtta acaacctcgc ggtaaatttt
    25021 gcccttttta atgatatagg atagattggc gctgtttgac atcttggtag aatattcctc
    25081 caccaaagct tccagctttt tttcaatctt tttattctcc tcctcgaact gccctggtgt
    25141 taaatcggta gggtatttct gaacatgcac catttgtacc atgctgtcga acctattggc
    25201 aaaaattatt gccagtctta acccgtttaa cgattccttt gaaaaatcga tgggtactat
    25261 tatatgtttc atggcattgg tggttggtta taagcaaatc taactaaaaa ttgaatacca
    25321 tttcaagttc agtatgttaa aatgaataat tattgaaccg tttttattca atttaacctt
    25381 aataaacaga tgtttacact taactcgctt aacttagcaa catcaggtag ctaactaaac
    25441 ctcattggaa tgatgacacg caaacggatt ggcatatgga ttttgctgtt ttcagttgca
    25501 ttagcaactt taacattggc cagtaggcca aaccctaccg aggaatccga gaaagaaaaa
    25561 cccgcaaagg gtacggccat catcattaca ggtgctgctg ctaaaatagc acaggaggcc
    25621 gctttactcg aaagccttta cgagcgtggc gaacttaacg atgttgtttt catttcaggg
    25681 gcgagttcag gtgcgcttaa tgcagttatg ctaaacgcag tgctcgagaa aaaacttaca
    25741 tggaaagagt atcgcgagct ttttggcgat attaaaaacg ataatgtttt cagaaccgat
    25801 aaaaagggct tgccggttag caacgagcct ttacgaaaac tgctggaaga aattgtggtt
    25861 aaccggctag gatataagac gttggccgac ttgccttttc caactgccat atcagtggtt
    25921 agcttacggc cttcgccatt gagtgaaggg acataccgtt tctgcaaccg taaaatcaat
    25981 cgtgaaagcg attcaaccct aaacctagtg gacgtattaa tggcatcaac ggcctacccg
    26041 cttgtttttc ccccatcccg aattaacggc ataaaagcca ttcccgatat cccttactat
    26101 gatggtggca ttggcgacga ccgtgttccc tttcaggcac tcattgagtt tgagaattac
    26161 aggaaacaac ccgttgaacg tgtaatcatt atttcgcgca agcgcgatac ggacatgacc
    26221 cttgccgatg agctaaaacc ttttgggtat gagctaaaaa ctaaattaaa taatggttta
    26281 tcgcccgatg tgctaacatc gctaggtttc tggcgaaggc ttgagcacat aaagaaacgc
    26341 taccccgaac ttgctgccaa aacccttgtt tttgttccca gctataggta tagcttcccg
    26401 atgttcgatt ttaacaacct caaagaacag tatgagctaa cctaccaatg ggcaaaacaa
    26461 aacaaaccca tccccctgaa cgagttcctg aaacaaaaac caacgcttaa taaaccctaa
    26521 aaaatgttat ttttgccaaa aatttaggaa aatggatatt gtacttagcg gaatacgctc
    26581 aaccggaaaa cttcaccttg gcaattactt tggagcgctg cgcaactttg ttcgcatgca
    26641 gcatgagaat aaatgcttct tttttattgc cgattaccat tcgcttacca cacatcctac
    26701 ccccgaggat ttacatggtt ctgtaaaaca ggtgcttgcc gaatacttag cctgcggcct
    26761 tgaccctgaa gtggctacac tttacataca aagcgatgta ccccagattc ctgagcttta
    26821 cctcttgctt aacatgaacg catacaaggg cgagcttgag cgatgcacat cgtttaagga
    26881 aaaggttcgc aagcaacccg acaacattaa tgcaggacta ctcacctatc ccaccctaat
    26941 ggctgccgat attcttattc acaaggcaac caaggtgcct gtgggcaagg accaagaaca
    27001 gcatctggaa atgactcgca cctttgccaa ccgattcaat agaatgtata aggtagatta
    27061 cttccctgaa ccggttgcct ttaactttgg tgaggattta gttaaaattc ccggcctcga
    27121 tggctcaggt aaaatgggta agtccgaagg gaatgccatt tacctttccg atgatcccga
    27181 gacaatccga aagaaagtga tgaaagcggt taccgatgca ggcccaaccc aaccaaatca
    27241 acctaaaccg gaggccatcc agaacctttt cactatcatg aaagctgttt catcgcccga
    27301 tacgctccag tactttgagg ataagtataa tgcctgtgag attcgctacg gcgatatgaa
    27361 aaagcagctg gccgaggata taattacttt tacaacacca atccgcgagc ggattgaggc
    27421 tatacttgcc gataacgatt acctaagaaa agtagcccta atgggggctg aaaaagctcg
    27481 cgaaagcgca tcgaaaacca tacgcgatgt aaggcacatt attggtttcc gagagtttta
    27541 ataaaaaagg gtgttgcttg gtcaacaccc ctttttgtaa aattccttgc ctaccctatc
    27601 gatatgctcc ttaagttcag gagtagagat tgaatggtag ttaaccacat caacatggta
    27661 gggaatagga attccctcat tcagaatacc agcaatgtct aaaattattt tgctgtttaa
    27721 actctcacca atcaaggcaa ggtcaatatc gctcccgttt ttataatttc cttttgctct
    27781 actcccaaaa ataattgcag tttctacctc aggataactt tttaaaacat caatgatcag
    27841 taaataactc ttctcggata taccaaatgt ttccattacc taagatcctt taatttagtg
    27901 tagagctgct caataccatg aataaattca tcgcgcacca tttggaacga ttgatttgca
    27961 gtaaattcat cgtaggtatg cgacatttgg ttccgttttt caagcatgtc gagccatagg
    28021 tctccatctt gaataagtcc gttagtgaat gcttccttta tggtatcgcg tggaaatctt
    28081 gcttcaacac ccatctcctg aaggtaatcc ttcagtgtct tccatccaag ttcaaaagta
    28141 aactcaaagt attgtatcac tccggctttc tccagttcgg atggagagtc atttcttaaa
    28201 gcgtccttta aacgtaaaaa cgctttttca aaattgtcga atcgttgctt ccacctaata
    28261 tccattttaa gtaatttatt tttaaactct ttacagattc tttttcaata ctctactcca
    28321 gctcccagtc gaacccatcc ttacggtcgc gaacaattac ccctaacttt gcaagctcat
    28381 cgcgaatccg gtcggatgtg gcaaagtcct ttcgctgttt tgcctccatg cgcatactca
    28441 gcagcatctc aaccagcttt gctgttagct cagcatgctt tgagcccgac gtatgctcat
    28501 caacaagtcc aaggatatca aagataactg ttctaaagaa cgattttagc tcatgtaaat
    28561 cggattcgct aatggtttcc ttgccctcgg caagcaggtt aatttgccta acccaatcga
    28621 aaagaaccgc aatggcaact gggctgttga ggtcgtcgtt taaggcttcg taaacctttt
    28681 cctttagcaa atcgacccta acacttgagg ttgccgatgg tttaatgcga tcgagctggc
    28741 ggtttgccgc catcaggcgg gcaaggccct tgtcggcagc ctgtagcgct tcgttcgaga
    28801 aatcgagggt ggaacggtac tgagcctgaa gcataaaaaa ccttatggtc attgggctat
    28861 aggcctgctc aagcaacctg tgtgtacccg taaaaagctc ctcaagggtg ataaaattcc
    28921 caagggattt acccattttc tgaccgttaa tggtaatcat gttgttgtgc atccagtacc
    28981 tcactgcttg cttgccgttg gccgctacgc tctgggcaat ctcgcactca tggtgcggga
    29041 aaagcaaatc catgccaccc ccatgaatat cgaaaggttc accaaggtac ttggtactca
    29101 ttgccgagca ctccaaatgc caaccgggaa agccttcgct ccacggcgat ggccaccgca
    29161 taatatgttg tggtgatgct tttttccaaa gcgcaaaatc gaacggattc tgcttctcat
    29221 cctgcccgtc gagctgacgg gtattggcca ttaaatcgtc aatattacgg ccggagagaa
    29281 tgccatagtt atgctttttg ttgtatttaa ccacatcaaa gtaaatagaa cctttccgct
    29341 cgtatgcaaa accgttatca atgatttttt taaccaattc aatctgctcg attatatgcc
    29401 ccgatgccct gggttcaata cttggtggca atacgttcag caacttcatg gcgctgtggt
    29461 acctatcggt atagtactga accacctcca tgggctcaag ctgctcaagc ctagcttttt
    29521 tggctatctt gtcttcaccc tcatcggcat cgttctccaa atgcccaaca tcggtaatat
    29581 tccttacata acgaacctta taccccaaat gcttgaggta acggaaaagc aaatcgaagg
    29641 tgattgccgg acgggcatgg cccagatgcg gatcgccata aacggtaggt ccgcaaacat
    29701 acaaacctac atgaggtggg ttaagcggct caaaaacttc ttttttgcgg gtaagtgtat
    29761 tgtaaatagc tatttggctc atatcaaact ggtaagattt aataagacaa aaattgcata
    29821 gaattattta attgccctaa actaaaggca acatcggaaa aagtgatgta tgtaatttga
    29881 tcgtttcatg atttgcaaaa gtaatgattt ttagattttt catgaagaag tcaggaatca
    29941 gaaatcggga gaccgtggcc aaaggccagt atagccctgt ttaacgggtt ctaacttcca
    30001 ttaacttccc catattcgtt taacgattca cggcttacga acccccatta acaatttacc
    30061 tttaaccatc accctctccc attttgattt ttcagcattt tgtattaggt ttgataaatt
    30121 ttaggagtat gaaccagctt gtgcggtata tcattattgg atttgtagta gctctggttg
    30181 cttttttgat ttggtacttt tcatcaatag tagcctacat actcatatcg gcagtgctta
    30241 gcctaatggg taagcctatt gtcgatttta tttcttcaat cagaatcaga aactggtcac
    30301 caccccgttt tttgggagcc accattgccc tcattacaat ctggatactt ttcatagcat
    30361 tcttcagggt aatgattcca cttgtaattg cgcagttcaa cgagttgggg tcaattgatg
    30421 tacaaagcct agtggcatca tttgccgaac caattaattc cattcaacaa ttcattcaaa
    30481 actatttgcc ggcaagcgcc caaggatttg tgctgcagga ttttctgata acaaaaatat
    30541 cggggatttt tacggtttca atgataacta atgccttctc atccacggct aacctgctaa
    30601 tcagcctggt tattgccttg ttttccataa gcttcataac tttctttttc cttaaggacg
    30661 acacgctttt ttttaaaagc gtaattctgc tattccctga aaaatacgaa atgcaaataa
    30721 ctcgagcgct ggttagtata aacaaactcc tgcgccgcta ctttgtgggt atagtaattg
    30781 agagtactgc cataatggtg ctcgatacca ttgggctttc cattattggc gttcgttttc
    30841 aaacagcagt ggttgttggc ttaattgcag gtataatgaa tgtagtacct tatgtagggc
    30901 cacttacagg aacagtgctt ggtacgctca tagtattggc tacacagctt cacctagggt
    30961 ttacagccaa catgcttaca ttcacaatcc tggtagtttc ggtattccta attacccaac
    31021 ttatcgacaa tgttgttttt caaccattaa tttacggaaa cagtgtaaaa gctcatcccc
    31081 ttgaaatttt tattgttctt ctggttgctg gtagtgttgc cggaatactt ggaatgttgc
    31141 ttgccatacc aagctataca gtaattcgtg tttttgctaa ggaatttttt aataacttta
    31201 gggttgttca aaaattaacc gaaaagatat agacagaata tcgattttgc ttaattttac
    31261 ctgataaacc atgccggcaa tgattaaaga tataacctca actgcagcaa ctattgcaat
    31321 agcaatgctt ttaccatgcc taagtttttc acaaaatttt gaagccgatt cggttttcac
    31381 atggcaacta gttgaaacaa acaacatcgg aagccaatca accctaaagt ttatacccag
    31441 aacaggcgta acaccaacag gttgggattt tggggactcc aacacatcga ccgaggttgc
    31501 acccacacac acatactcct atacaagctg ggacgatagt gtaaccgtta ccctgaacta
    31561 taccctgaat ggcgaaagcc tatcgcactc acgcgagatt cctgttagcc ctgctttctt
    31621 ttttgttttc cccgacagaa atttaggccg acttgcctcg tacaaacgca ttttccttaa
    31681 tgcttaccga atacccaacc accccgatag cttgggaaac ctccgcttca cctggacggt
    31741 tgatggtagc cctctggccg gaaacgcttt cgacgatgta gcgctcgacc agtggccaaa
    31801 catttactat acctttacca ctgcagggtt acatcaggta aaacttgatg tgcgtaattc
    31861 aaacagcgga caaacagccg agtttactca aacaataaga gtcctgcccg actttactag
    31921 cggaaaggtt ccgcttgaaa atatccctaa cgtatttacc cctaactccg acggggtaaa
    31981 cgataacttc attgtaccca ccagcggcac agcatggttc gattttcgta tattttcccg
    32041 ttctggcggt ttaatttaca aagcggaatc gagtgtttta gtatgggatg gcaagaacaa
    32101 ccaaggtacc gatatgcccg aaggggttta ctactacgtt atagaagacc tttccgggca
    32161 atatgatagc gctaaaggtt ttgtatacct atttagaggg aaaaagtaaa gtttaatgaa
    32221 tagaattcct caattcatac tgttatttgc acttttgctt acctcatgca ataacattgt
    32281 atttaacaag cctcagcctg ttgacgggaa aatcatcact aacctaaaca atgcatttcc
    32341 gggaattttt atcagttccg ataacgacac gctaactatc accaggaata ccatatcgct
    32401 tggcagtgga aacaaattta ctgtaaccgg aaccctagga aaaaaccttg atgttaggga
    32461 atattccaat gggtttgttg ttaacttttc cgatagtgtt aagggtcgat tggtatggat
    32521 tgcttacatt tttaagctaa gcaacgattc actttttatt agcttttccg atttcgaccc
    32581 aaacaagcta actgaggttg aaaaggaaat aggcaacaaa gtgccttacg agaaaattaa
    32641 agacgaaaac aaagagaact cgaagattat cctaaaaccg aggaattcga gagagtttga
    32701 caaacttgtt agcgcaggaa ttttcaataa aaccatctcg atgaagatgg aaaaacccaa
    32761 gccctaaagg gcttactgtt ccacccactt aaaccacacc ctttggtcga aataggggat
    32821 tccatttttt tcacacacac gcatggtgtg cccagtgcca cccgttttgg gtttcgataa
    32881 atcatcgtaa aaaatggcta aagttgcagg ctgcaactta gaggtaccca ccacctttat
    32941 ggtatcgcgg agtaaatatc gggccttaac ggttagagca ttatcgccaa gtgaaagaaa
    33001 actatcgata agctgcgata cattgctgct gttcttggtg tgaaatatta cgttaggttc
    33061 acttgccagg tcaacatcgt cgagcgaaac cgtttgcctg cctaaagtct ctttcgttct
    33121 atggccgcgg tacggggtta cttgctgtaa gcgcgatggg ttaaccatcg aaactccctg
    33181 ggaaaagaag taatcggcgc ctttggcatt tccgctcctg aaaacaatat gtttactgct
    33241 gcctgcgagc agcttaccaa cctcaattag cttgtgctgg tcggtaggta aaacggcacg
    33301 tttaccctca aggaggatga ccgtgccggg tttatcataa ctttcaatta gctgctcaat
    33361 ggtaagcatt gtaaactcta tcccctgtag ctaatccact taatcatctc tttccaggtg
    33421 tactttttac cgtacattag tatgcccacc ctgtatattt tggcggctag ccaggtaata
    33481 aggataaatg tacccacaag cagagtagcc gatagtgcca actgccaagt tggaacgcca
    33541 aaaggcaaac gggttagcat aataaccggc gaggtaaaag gaattagcga gaaccagaaa
    33601 gctaaaggcc catcgggcga cttaattccg ctaatcatca caattattgc cagtatcagc
    33661 ggtagggtaa ttggcagtgt gagctgttga gtatcggtta tactttccac tgctgagcct
    33721 acggctgcaa acatagccgc ataaagtaag tacccaaaaa ggaagtagaa tataaatcca
    33781 ccaatgagta atgggaagtt ctggttcttg agggccgtaa ggaaactcat tccctcactg
    33841 tttgcaactg cagtatcctg ttcctgaacc tgtgccattt gggggtcagc ctgggccatg
    33901 gcctgcattt gctcggtagc tggttttgtg gtcattttac tgcttatgat gttgatacca
    33961 accccggcaa gagcagcggt cattaccacc catatcagta tttgcagcag gctcactgag
    34021 gccatcccaa aaattttacc agccatcatc tgaaacggct taaccgatga aatgataacc
    34081 tcaaccacac ggtttgtttt ctcctcaata accccctgca taacctggtt accggtaata
    34141 agcaccatca tatacatcaa aaagctcatg atgtaagcta ttgccatagc aattagggtg
    34201 ttactctcct tttcctcacc tgtttggctt acctttatcg attttattga gatacgggtt
    34261 ttaacatcgg caagtatctt atctagatta tcgatattat aggttttaag cttgcgcgcc
    34321 tcaatttcct tttctaaagt attctccagg tgctgcataa catcaatggc aggctgtttc
    34381 tgggagtaaa tggtgaaagc tgcctgatta ggtgtggagg tagagtcaat caccaatagg
    34441 gcataatagc ctgtttcatt taagttttcc ttaaccttct cggcaggagt atcggttacg
    34501 taagttacct tgatatactc agttgaggga attacatcaa tatactgttt ggtattatca
    34561 accacggcaa cggttcgtac ctctgtaacc tcaagcgaag ctaggtagct tggaagtata
    34621 gctatggctg caaaaaatat tggcgagagc agggtggtta tgataaacga ttttttcctg
    34681 accctggtca tgaactcgcg ctgcattatg agaaatatct tgttcatact attttctagt
    34741 ttaagtttta acctaactga ttactaaacc gtgcttttgg ttatactcct tcactacctg
    34801 tataaaaata tcgtgcatgc taggcacaat ggggttgaag gaaactattt caacagctgg
    34861 aatcaattcc tgcaaaaggc tattggcatc ggattgcgat tttaatctaa cctgcgcctt
    34921 aggtagcgag ttgtcggtat caatcttcaa cagttcgaac ctatcttcaa ccttttgcct
    34981 tagtgaatcg ggattgccac ggaaagctat ttcgaaaaca ttgctaccgt aactctggcg
    35041 cacctcatca atttgcccac ttaggatgtt acgcgacttg ttgattaggg tgatatggtc
    35101 gcaaagctcc tcaaccgatg ccatattgtg ggttgagaag ataatggttg ttcctttacg
    35161 tttcagttcc agtatttcgt cctttacgag ttgaacgttt atggggtcga acccgctaaa
    35221 cggctcatca aaaatgagca gctcaggctc atggataacg gtaaccataa actgaacctt
    35281 ttgagccata ccctttgaga gctcctcaat ttttttatcc caccaggcct gaatttcgaa
    35341 tttctcaaac cagtacttta accgcttcat ggcctcagca cggctaagcc ccttaagctg
    35401 tgcaaggaat agggcctgtt cacccacctt catcttcttg taaagtcccc tttcctcggg
    35461 taggtaacca atacggtaaa tatcgttagg ttgcatgtgc cgaccatcaa atagcacctc
    35521 gcccttatcg gggccagtaa tttggtttat gatacggata agggtagttt tacccgctcc
    35581 atttggcccc aaaaggccgt agatgctacc ccttggcacc gatatgctaa catcgtcgag
    35641 cgccaaatga ttggagtagc gtttctctat tcctttagct tcaagtaaat gcatcgcttt
    35701 tcaatgattt ttgtaagtac tgtttatttt gacacgtaaa gatataaagt agaaggattc
    35761 ctgcaaaaaa tgtagggaaa aatagctttt ttattaatga agcattatat actgtccaat
    35821 caaagttctt tatcgaaatg tcgtttagaa tacaaaacag cccctccttt tgtcatgttg
    35881 tgcaaagtga aggatataat tcttagtgta cagt
//
LOCUS       DAOOWJ010000015        35246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1247_length_35246_cov_8.069279, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000015 DAOOWJ010000000
VERSION     DAOOWJ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 35246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35246
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1247_length_35246_cov_8.069279"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(847..2106)
                     /gene="nqrF"
                     /locus_tag="PLW22_02835"
     CDS             complement(847..2106)
                     /gene="nqrF"
                     /locus_tag="PLW22_02835"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOU73671.1"
                     /translation="MILLSGNGTIIISAIAVFLLIILLLVAVLLYAKTKLTPQGRVRL
                     VINEEKTLEVDPGGTILSTLSNNGVFLPSACGGGGTCGMCKCRVLEGGGSILPTEVGF
                     FTRKQQNDHWRLGCQVKIREDIKIEVPEEVLGIKKWECEVVSNHNVATFIKEFVVKLP
                     EGEKLHFRSGGYIQIDVPKIEVDFAKDIDVEPEYRDEWDRYKMWDLKMKNTEETFRAY
                     SMANHPAEDNIIMLNVRIATPPWDREKNAFMNVNPGVCSSFIFSRKPGDKVMISGPYG
                     EFFIKDTQNEMMFIGGGAGMAPMRSHIFDQFLTKRTTRKATFWYGARSKREIFYEEHF
                     RKIEQEFPNFKFTIALSEPKPEDNWTGYVGFIHQVIYDNYLSKHDAPEDIEYYLCGPP
                     AMNAAVQKMLFDLGVPEEMIAFDDFGG"
     gene            complement(2128..2745)
                     /gene="nqrE"
                     /locus_tag="PLW22_02840"
     CDS             complement(2128..2745)
                     /gene="nqrE"
                     /locus_tag="PLW22_02840"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOU73672.1"
                     /translation="MEHLINIFVKSIFVDNMVFAYFLGMCSYLAVSKTVKTSMGLGIA
                     VIFVMAITVPVNYLMNKYLLLPGALNWLDPGFANLDLSFLSFIMFIAVIASMVQLVEM
                     VVEKFSPSLYNSLGIFLPLIAVNCAILGGSLFMQEREYETLAEATVFGVGSGIGWALA
                     IVSIAAIREKLKYSDIPKPLRGLGIAFILTGLMGIAFMTFMGIKL"
     gene            complement(2768..3427)
                     /locus_tag="PLW22_02845"
     CDS             complement(2768..3427)
                     /locus_tag="PLW22_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOU73673.1"
                     /translation="MSEVKEREPWFSPKNIKLLTEPIDSKNPVTVLVLGICSALAVTV
                     KLKPAIVMAISVTVVTAFSNLIISLIRNSIPNRIRIIVQLVVIAALVILVDQILKAYV
                     YDVSKQLSVFVGLIITNCIIMGRLEAFALGNKPWPSFLDGIGNGLGYGLILVIVAFFR
                     ELLGSGTIFNYRVIPIDWYIKNGGFYENNGMMILPPMALITVGIIIWIQRSRNRKLIE
                     D"
     gene            complement(3420..4193)
                     /gene="nqrC"
                     /locus_tag="PLW22_02850"
     CDS             complement(3420..4193)
                     /gene="nqrC"
                     /locus_tag="PLW22_02850"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOU73674.1"
                     /translation="MSLLDKIKSMDRNSNAYTIMYSSVMVIVVAMLLAIASTALKPAQ
                     TKNIETEKKIDILRSIGKADGVEQAANKHAFVSENFNKYIPEQVVVNSKGDTITGVVA
                     FNVDLKVEMSKPIEQRNLPVYKAKLDDGSIKYIIPLRGRGLWGPIWGYIALNDDLNTI
                     YGATFAHQGETPGLGAEISTHEFQEQFKGKSIFQGDSILTFTSVKVVKGGAAPNDPHG
                     VDAISGGTITSKGLESMLFDCLAAYQSYFQKVKNSISHE"
     gene            complement(4206..5372)
                     /locus_tag="PLW22_02855"
     CDS             complement(4206..5372)
                     /locus_tag="PLW22_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOU73675.1"
                     /translation="MKALRNYLDKIKPHFQKGGRFEMFHSTFDAFETFLFVPNTVTTK
                     GSHIRDSLDLKRTMSIVIIALMPAMLFGMWNVGYQHALSIGASWGFLETFWYGFLKVL
                     PLIVVSYGVGLSIEFAFAQSRGHEVNEGFLVSGLLIPLIMPVDVPLWMLAIAVAFAVI
                     IGKEVFGGTGYNVFNPALLARAFLFFAYPSHMSGDKVWIAGLTQGKGVVDGFSGATPL
                     AKAAAYQIDQLPSVYDMFMGFIPGSVGETSTLAILIGAIILIVTGIGSWKIMVSVFGG
                     GLAAGLLFNAFAVNPYMEIPAWQHLIMGGFAFGAVFMATDPVSASQTEKGKWIYGFLV
                     GFLALLVRVLNPAYPEGMMLAILLMNAFAPLIDYYVVEANIRRRMKRAKKVANV"
     gene            complement(5389..6747)
                     /locus_tag="PLW22_02860"
     CDS             complement(5389..6747)
                     /locus_tag="PLW22_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOU73676.1"
                     /translation="MSKPIKIKRGLNIPLKGSAEKVLVRLDKAETYGVKPTDFPGLVP
                     KLNVAVGDKVRAGSPLFHDKYRPNIVFSSPVSGEVVDVRRGERRVILEVVVKADSGEV
                     AYEDFGPASPETLTRDEVIAKLLKAGAWPYIRQRPFGIIANPADSPKSIFISGFDTSP
                     LAPDLDFVVQGEEESFQAGVDAIRKLTTGKVHLGLSSDFPPAKAFANAKGVEFHYFTG
                     PHPTGNVGVQIHHIDPINKGEVVWVVNPLDVIIIGRLFLKGIYDATKVIALAGSEVVK
                     PRYYKLISGASIESIIKDNINTQGQVRIISGNVLTGTRVDKLGYLSFYDNMVTVIPEG
                     NKYEFFGWIAPGVNKHSASSTFLSKLIPGKRFTLDTNMHGGHRAMVVTGQYEKVMPMD
                     IYPMHLVKAILANDIDRMEQLGIYEVVEEDMALCEYVCPSKTEVQAILRKGIETMIKE
                     LS"
     gene            6953..8179
                     /locus_tag="PLW22_02865"
     CDS             6953..8179
                     /locus_tag="PLW22_02865"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOU73677.1"
                     /translation="MAENVFGKPEKLSNQWKTNIKSVKAYKTGFELTQPIIELNTDET
                     ITLEFDDLSDETEQYEYTVIHCDYSWNPTNMVFMEYADGFDFNPIHDYRYSSGTLVQY
                     RHYTLTIPNSNLKLKLSGNYIVQVVRQSNRSEIVLQQQFRVYEPLVKVDALIRQPIEP
                     SIQSTHQQMELTVNTALLGKVDPNNDVVVLLNQNNQPYNQLTFKTPHFSDGHMLIYHT
                     PTSPIFEGGNEFRQLNLKSFHYQVAEIQGIKQYDGVYHTILTPDKRVKTYRYSESADI
                     NGKFIVKCDDVTESQEEADYTWVYFTLSNDVQFDGDIYIFGELSGWELNPNFKLKYNP
                     ECNCYETRLLLKQGFYNYKYVFVSQGLATPDFNRIEANFFETENAYNILVYYKSQGAR
                     YWRLVGLNGVNSRYKN"
     gene            8432..9280
                     /locus_tag="PLW22_02870"
     CDS             8432..9280
                     /locus_tag="PLW22_02870"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU73678.1"
                     /translation="MRNKILRFAVLAIFASAIAVSCSKEDDELVQKPNESQWTVRPDF
                     ATLDTRPQDIIEQFTLTEKDADLVKVDENTPKGAKYALVIGISDYSGTQYDLQYCDDD
                     ATDWKNRLVKEGYSVTILLDRNATSANIQSAINSLVSKAAAGTEIAFCYSGHGSNGNI
                     VTTDLYYISSSWFKTKFSTAKSTKMMFCFDACQIGAMKTALKATGRVVAVASSTTTYS
                     YDGDATMKNGVFTYYQMYGFDYKGYIYLEPDCSYACTQMKNWASARGLTVAPSYVDSY
                     SGNFDL"
     gene            complement(9358..10659)
                     /gene="mtaB"
                     /locus_tag="PLW22_02875"
     CDS             complement(9358..10659)
                     /gene="mtaB"
                     /locus_tag="PLW22_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOU73679.1"
                     /translation="MNQVRKRVAFYTLGCKLNFSESSTIARQFADMGYERVSPDSEAD
                     VYVINTCSVTEHADKKCRQAIRKFTTKSPNAMVAVVGCYAQLKPEEIAQIDGVDFVLG
                     AADKFNLPHLIGNDAKKTKVKVYSCDIDSVSDFFPAYSTGDRTRSFLKVQDGCDYHCS
                     YCTIPLARGKSRNMPISQIVDQAKAIASQNVKEIILTGVNTGDFGKSSGETFLELIQQ
                     LDRVEGIERFRISSIEPNLITPEIVDFVAQSAKFLPHFHIPLQSGSNRILALMRRRYR
                     RELFAERIELIRSRMPDTFFGVDVIVGFPGETDDDFSDAFRFIEQVKPSFLHIFPYSE
                     RPNTPATEFEGKVSPKVVKQRVTLLTVLSDRLHKEFFLKHIGQIRPVLVESSRKGDYM
                     FGFTDNYIKVGIPFDKGHAGKVVKVRLEKFTPDGFVEGLVI"
     gene            complement(10740..11645)
                     /locus_tag="PLW22_02880"
     CDS             complement(10740..11645)
                     /locus_tag="PLW22_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HOU73680.1"
                     /translation="MKILKFAAIDIGSNAARLLLTNVVEDGETVVFSKSSLVRVPIRL
                     GEDAFSIGYISEHRAQKLLKTMIAFKNLMEAQDVVAYRACATSAMRSSSNAPELVSMV
                     KQHANINIEIIDGSREAEIIYANGIAEMLDHDRPYIYVDVGGGSTEITVFEKGEIKAS
                     NSFNIGTIRILKGMIGKPDFDQVRDWLKSVVKKKDQPKIIGSGGNINKLYKLARKPIG
                     EPLYQKEVKNLYDFLDSFSVEDRIKFLSLNPDRADVIIPATKIFLKVMKWTGAKEVIV
                     PTIGISDGIIRTLYADYKQSLALQQ"
     gene            complement(11642..13726)
                     /locus_tag="PLW22_02885"
     CDS             complement(11642..13726)
                     /locus_tag="PLW22_02885"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOU73681.1"
                     /translation="MAPKKIPLVNRELSWLTFNGRVLQEAANPSVPLIERIRFLGIFS
                     NNLDEFFRVRVATLRRLLVVEKGAKKIMGDNPRKILEKIQRIVIDYQKKFDNTFQEII
                     DELRKENIYLINETQLSAGQIDYLNGFFDDHISEFVAPVILKRNNKFPELVDQSIYLA
                     VKLTNSQKPNTKEFALIEIPSRFVKRFIQLPNEGDKSFIILLDDVIRLCLSKVFKILP
                     FDTFEAYTVKITRDAELDVDNDISESLLEKISKGVKNRKRGEPVRLVYDEKIPTDLLK
                     FIVKGLNLDEDDYVIPGGRYHNFKDFIRFPSLGRNHLINLPLEPVSVKELDEATRIMD
                     IMSDHDFMLHYPFHSFQYYIRLLREAALDPAVKSIHITLYRVATESRVVHALINAAQN
                     GKKVTAIVELRARFDENANIYWSKQMQEAGINVIFGVPGLKVHSKMTLITRQEKNKER
                     KYAVVSTGNFHEGNANIYTDVNLFTADSRITSEILKVFTFLETNYKTFSYKHLLVAPF
                     NMRRRLYSLIDTEIENAAKGLPAYLIGKINNLVDEEMIKKLYQASKAGVKIKLVVRGT
                     CALVPGVKGISENIEVYGIVDRFLEHSRIFIFANGGSEVCYISSADWMTRNLDYRIEV
                     ATPIYSKPLIEEMKTVVNYALRDNVKARLVNHGGCNEFKPCKEGEPEFRSQLELYSFY
                     QNIERHKNGEVK"
     gene            complement(13803..14291)
                     /locus_tag="PLW22_02890"
     CDS             complement(13803..14291)
                     /locus_tag="PLW22_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU73682.1"
                     /translation="MAKTLLILRHAKSSWAEADKADKDRALKAKGISDIVNTARAIAP
                     KVQGLDLIITSSANRAVHTAILFAETIGFPHDKIRIEPMVYQADERELVEMIKSLPDE
                     YKSVMVVGHNPTLTYLVNTFVANRIHELPTSGLVGVKFDINSWIELSAEKVYSVFQSF
                     DW"
     gene            complement(14309..15637)
                     /locus_tag="PLW22_02895"
     CDS             complement(14309..15637)
                     /locus_tag="PLW22_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOU73683.1"
                     /translation="MAKSKVNKIFVIDTNVILHDFQCLYKFQDNDIVIPIVVLEELDK
                     FKKGNEMINYHAREFVRELDKIAGDKLFNGGLPLGKNLGKLRIETGKPMPPEMKESFA
                     EFTPDNRILAITIHLVKHNPERPVILVTKDINLRMKAKSLGIVAQDYLNDKVEDIDSL
                     KKEIEIIEKVDDALIQKLYNSEEKLLAKDLKLKPYANQYYILKGNKSSALAHYDKATD
                     TLDRVEKVRAYGIDPRNAEQTFALDALLRPDIQLVALTGKAGTGKTLLALAAALQQEE
                     QFDQILLARPIVALSNKDIGFLPGDVDEKIGPYMQPLFDNLGVIKNRFKPTSKDYMHI
                     EEMQRADKLVITPLAYIRGRSLSNVFFIVDEAQNLTPHEIKTIITRAGEGTKFVFTGD
                     IHQIDHPYLDMKSNGLTYLSDRMHGQEIFAHINLVKGERSYLAELASDLL"
     gene            15777..17060
                     /locus_tag="PLW22_02900"
     CDS             15777..17060
                     /locus_tag="PLW22_02900"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOU73684.1"
                     /translation="MTYNEALDYIYSHLPIFQRVGKAAYKADLGNTLKLDEYFGHPHR
                     TFKTIHIAGTNGKGSTSHMLAAVLQKAGYRVGLYTSPHLRDFRERIRVNGEMIPEDEV
                     AKFISQHKDFFDQIMPSFFEMTVALAFDYFSRMNLDVAVIEVGLGGRLDSTNIINPIL
                     SVITNIGFDHTDLLGDTLPKIASEKAGIIKPDTPVVVSKYQPEVENVFIAKAKEMNAP
                     IYFADQEIQVYEQYIDTTGLQVLKLADKVRDIKIEIDLLGNYQRYNISGVLKSIEILS
                     GKGFLLSNEALMEGLRNVQTLTGLMGRWQKLSENPLIYCDTGHNVDGIKYVVEQLRHI
                     PHNNLHMVIGVVADKNIDGMLALLPKNATYYFTQAAIPRALNHEELKKRAAEHQLYGH
                     SYSTVALALAAAKEASNPNDLIFVGGSTFVVAEVV"
     assembly_gap    17270..17369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17556..17630
                     /locus_tag="PLW22_02905"
     tRNA            17556..17630
                     /locus_tag="PLW22_02905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17591..17593,aa:Val,seq:tac)
     assembly_gap    17656..17755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18203..19486)
                     /gene="murF"
                     /locus_tag="PLW22_02910"
     CDS             complement(18203..19486)
                     /gene="murF"
                     /locus_tag="PLW22_02910"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOU73685.1"
                     /translation="MIEHIYKEFEKSYHVTIDSRKVKAGSIFFALKGENNDGNAYAAN
                     ALQDGAALAVIDNPHYMTKGCVLVENALNALQQLANYHRQKINAQIFAITGSNGKTTT
                     KELLYTVLSQKFKTYATVGNLNNHIGVPLTLLSITPDVDFAIIEMGANHPGDISELCR
                     IAEPDFGLITNIGKAHLGGFGGFEGVKNTKGELYNFLALRDRAIFYNHQNPILVELLN
                     KYKANNVIPYSKYFEDFIVVEKPESPFLSISISEGGKLTTILTNLVGQYNVENVMAAY
                     AVGRHFGVGPGTIKDAIESYIPSNNRSQLLKTQNNTVIMDAYNANPTSMENALKNLAK
                     LHASKKVAILGEMLELGEYSLDEHKKIVELASALNFDKILLVGKGFSSFNGMFYYFDN
                     SSECAEYLKQNPIKDSMVLVKGSRGVKLEIVVEAL"
     gene            complement(19554..21086)
                     /locus_tag="PLW22_02915"
     CDS             complement(19554..21086)
                     /locus_tag="PLW22_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU73686.1"
                     /translation="MSLKYRILLLASAGIFGLNSCGLFGGGGGGTGASEKTGWEYNSP
                     DNGGFEVVENWRPEAGPGLVFIEGGTFTMGRVEQDVMYDWNAKPRRVTVTSFYMDETE
                     ISNVDWREYVHWIKRVYSTYPQVIKNALPDTLVWRSPLGYNEPFVTDYFRHPAYNDYP
                     VVGVNWLQANDYCIWRTDRVNERLLVDKGIIEFDLAQKGADAFSTEAYLSGQYDARVK
                     KLLPSLDPEKPEGRHVRFEDGILLPKYRLPTEAEWEFAASGLVGNTYDERMVERRIYP
                     WNGHNVRNPESKVRGQMMANFVRGRGDYMGLAGNLNDENTFPGPVKSYWPNDYGLYCM
                     AGNVNEWVLDVYRPLSFEDVDEIRPFRGNVFKTPYDLDGNYSKKDSLGRIPYREVTEE
                     EAANRQNYNKAYYINYKDGDVASTLDFRDGQTVKEKNSDRMYYQGDTQQKNVRMTTLI
                     NDHVRVYKGGSWKDRAYWLSPGTRRFLDEASSTNDIGFRCAMEAVGAQSAKKRGERIG
                     GK"
     gene            complement(21169..22206)
                     /locus_tag="PLW22_02920"
     CDS             complement(21169..22206)
                     /locus_tag="PLW22_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOU73687.1"
                     /translation="MISKNSFFLLYLLGLVLCFEKSHGQDPVFSQVFFNPTMLNPSYA
                     GASKDMRLGIIYRNQWTMIDMPYSTFGVSFDRRIDFGPSWKHSFRNNRSQSSGRSGFG
                     INIMSDVEGKGTFSRNTLDLIYSYGIQPTYNSHVRFGLQTSAIFRTRSFSGLVFPDMI
                     DPAGNVIGNSDAVGYTTWNYDIGLGVAGDFENFYGGFAVHHLLQPVEASFSGGKAYIP
                     RKYTFHIGADFNLYKWRRFKEVLLFSPNVIYIQQLNFNQLNLGFYLSKNWAVAGLWFR
                     ENLSLNSHSFVVTLGYADYMFRIGYSYDFSIMQHGLRGLPTSSHEVTLGWNFEYKRGK
                     KKYRYIKCPKF"
     gene            22310..26062
                     /gene="porU"
                     /locus_tag="PLW22_02925"
     CDS             22310..26062
                     /gene="porU"
                     /locus_tag="PLW22_02925"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOU73688.1"
                     /translation="MRAVFFIILLTTQALGAFTQEFHRILEWVETDKKNINCLWFNGE
                     DFPDPASKVPYYTELIPIPDSKEYRIEITHIKWGKLNRDFDSSIVQTEPNPTTKSLRV
                     GANRVLLFSIPAMRNNASGSIEKLESFSFRIEPISSHEKNIDKTIRTSLSNSVLASGK
                     WVKVGVQKSGIYRISFEDLQRYGFSNPENISIWGIGGKSLPLMNAQSNLDEVAPIPIQ
                     ISKGADGIFNQGDYILFYAQGPETLTYNETYSAWKWEKHGYTKTIYYFLTTSQPQSII
                     GFADTPTETENYATNEFDQVTTFESNDTNLVKSGREWFGEIFDIKTTYSYNVSTPLPV
                     NNSTYKVWVRVAARSSSTSTFTLTNNGTLGSITLNSVIIGDELANVVSVNEKVFSGNT
                     VGQNLTFDLTYNKPNSGSVGFLDFISIQSRHGLLYSNAQLPFQDFQSVGAGRISRFTI
                     SNANSSVQVWDITDINEAKRVNISYANGTITFKQATNTLKTFIAFEPDKAYSVVGYAN
                     VDNQNIRGSSFFDYFIVTHPSFKTYAEELAQLHRQRSNLSVAVYTTDQVYNEFSGGNP
                     DVAAIRNMLRYFYKAATSDDDKPKYLLLFGDGSFDNLSNSQVNTNFIPTYQSPRSINR
                     VESFVSDDFFGLLDDNEGEASGYIDIGIGRLPVSSPEQAANLITKIKSYMENEDIDDW
                     QSKLVFIGDDEDGNVHMQDANTLAKYIETNQPAYNILKIFFDAYQQEVVSGGQRYPDV
                     TNAINSAVNNGALLLNYTGHGNERWLAHEKVLMLNDVLGWKNAKRLPLFVTATCEFSR
                     FDDYHMTSTGEWILLTPKGGAIALLSTTRLVYSSPNFTLNYNFIQQLFKTDSNGNYLR
                     LGDLVRITKILSGTGYNKRNFTLLGDPALMLKYPTYNIKISYINERPASEPADTLKAL
                     NKVNLKGFIADNLDTTVENFNGTVSVTLYDKEREITTLANDGGTPMTFKSRDNIIFKG
                     KATVSQGTFSIDFTVPKDINFSYGHGKISLFATDGMQTALGVHDSIVVGGIGNPESID
                     SEGPQIKIYLNDTKFTNGGICNSNPLLLVYLSDASGINTTGAGIGHDLTATLTYPAGQ
                     SETFNLNNFYKANTDDYTQGIATYQFSGLTPGTYTIEVKAWDAFNNSNTGKIVFRVIG
                     DDKLVVGNFYCVPSPVKDNTQFYFETNTNETGLDVKLEIFNINGSKVAELLKKRIYTE
                     GYRIGPLEWNGCNSNGEKLAKGIYFARIQVSSVQGEQVATTKFVVL"
     gene            26152..27345
                     /gene="porV"
                     /locus_tag="PLW22_02930"
     CDS             26152..27345
                     /gene="porV"
                     /locus_tag="PLW22_02930"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOU73689.1"
                     /translation="MIKKFFGIATILLVGLQPTVKAQFDSTISLSGGLNALRIAVPFL
                     TIAPDSRSAALGDAGVASVPDVNSQHWNPAKFPFIEGKKWGMGFSYTPWLRNLVNDIN
                     LTHLTGYYQFDKMQSISGSLLYFSMGEITFRDNNNNYIQAFNPNEFAIDFAYSRKFSD
                     HIAGAMAFRYIRSDLTGNFTTTGTGSGKPGTSFAADLAVYYQNAINLAGKSGEMAIGG
                     NISNIGTKLSYNEDPQKEFIPINLRVGGRLSLNIDQYNGFSYLLDLNKLLVPTPPVYN
                     SQDEIVSGMDPNVSVVQGMIQSFYDAPGGFKEEMQEIYIATGVEYWYARQFAIRAGYF
                     HESQNKGNRKYITLGAGLRYNVFNIDFSYLIPTGGFNSPMANTLRFSISFEFEPARTR
                     TNRDK"
     gene            27345..27824
                     /gene="ispF"
                     /locus_tag="PLW22_02935"
     CDS             27345..27824
                     /gene="ispF"
                     /locus_tag="PLW22_02935"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOU73690.1"
                     /translation="MDFRIGFGYDVHQLQQGLPFWLGGVQIEHTKGSLGHSDGDALIH
                     AICDAMLGAANLGDIGVYFPDTDPSLKGIDSKILLRKTMDIIAQKGYTIGNIDSTICL
                     QRPKVKDYIPKMQAELAKALDIEPQQISIKATTTEKLGFVGREEGVSAYAVVLLRKN"
     gene            27829..28218
                     /locus_tag="PLW22_02940"
     CDS             27829..28218
                     /locus_tag="PLW22_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOU73691.1"
                     /translation="MEAGNDILPKTLVLGASQKPERYSYLAIRSLRKHGIEVVAIGAR
                     KGMVDDIEIQTELQDIPDIHTVTLYLSARNQAPYYNYIINLKPKRLIFNPGTENPELE
                     KLAREHGIDVVEGCTLVMLSNGTYYEW"
     gene            28212..28541
                     /locus_tag="PLW22_02945"
     CDS             28212..28541
                     /locus_tag="PLW22_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU73692.1"
                     /translation="MVKVIQNESDLSKALSEPASAIYFNSQACGVCHVMLPQLEQIVG
                     SEFPKMHIYVVNANETPEICGKFSVFTFPTVLIFFEGKESLRMARNINLATFRQAVAR
                     PYSILFD"
     gene            complement(28538..28942)
                     /locus_tag="PLW22_02950"
     CDS             complement(28538..28942)
                     /locus_tag="PLW22_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73693.1"
                     /translation="MKRTAQLLLFIATFLLLSGSSCDKYEKGEGITGVWRCRENYQGT
                     KYRSYNVSVDRYTQLDSSTYVIYNLYNLGLDVETYVQLKDTVFTVLSTNSDLYFITGR
                     GVWHRYSQTIDWEYSVSGQVNDPFISATFDRP"
     gene            complement(28971..30986)
                     /locus_tag="PLW22_02955"
     CDS             complement(28971..30986)
                     /locus_tag="PLW22_02955"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOU73694.1"
                     /translation="MTLEEFRKDILQGIPDELPAPKPWDPSVNHAPKRKKILTLEEKK
                     LAVKNALRYFPPKHHAVLAEEFARELEEYGRIYMYRFRPDYKIYARSINDFPHKSVQA
                     ASIMLMLSNNLDYAVAQHPHELITYGGNGAVFQNWAQYRLTMKYLAEMTDEQTLVLYS
                     GHPMGLFPSHPDAPRVVVTNGMMIPNYSSKDDWERYNALGVTQYGQMTAGSFMYIGPQ
                     GIVHGTTITVMNAGRKQMKPGETDLRGKIFVSSGLGGMSGAQPKAAVVAGMVGVIAEI
                     NPKAVEVRHGQKWVDEVYTSLDELIPRIRQASANKEAVSLAYQGNIVDLWERLVTENI
                     HVDLGSDQTSLHNPFAGGYYPVGLSFEEANRMMAEQPELFKEKVYESLRRHVKAINTM
                     AARGMYFFDYGNAFLLESSRAGADILKPNGDFRYPSYVQDIMGPMFFDYGFGPFRWVC
                     TSSDPKDLATTDAIALEVLEEIAKTAPAEIMGQLQDNIHWIREASKNNLVVGSQARIL
                     YADAEGRIKIAAAFNKAIRDGRISAPVVLGRDHHDVSGTDSPYRETSNIYDGSRFTAD
                     MAIHNVIGDAFRGATWVSIHNGGGVGWGEVINGGFGLTLDGSDAADRRLHMMLHWDVN
                     NGIARRSWARNSGAMLAIKREMERTPLLKVTLPNIADDSLIDKVVKG"
     gene            31399..32301
                     /locus_tag="PLW22_02960"
     CDS             31399..32301
                     /locus_tag="PLW22_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOU73695.1"
                     /translation="MSTIAITDCEGNVLYSLEECEMFPNYTAVLKDAIAQGKDLTGLC
                     IKNQNISNVVFQGTTLYNAVFENCNFHKVEFIECQADSVWFNRCNLDGLKVKKTNLSK
                     AQIHKCSISKAHFTRSNLNDSLIFDSNLHLAVFLKTQLNNVGFDNCNLEKAKFEECSL
                     AEAGFYNLSPTYQWINDVLFSRCILNNCNLSSLNSLSMLFIRESNIEDAELQDQNFTR
                     VINTYTKVLYAIESDTVWWEDFRGNFEQFKDMVENDTMENGTKYYLDDFLYDELSKVV
                     YYLEQWHNEQTIIAQNTLSINKCE"
     gene            complement(32390..33526)
                     /locus_tag="PLW22_02965"
     CDS             complement(32390..33526)
                     /locus_tag="PLW22_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73696.1"
                     /translation="MARVKDINPFLVNSYISKEYFCNRENELNVLLRNVRGNVNTTLI
                     SPRRLGKTGLILRFFEMLGEMGDYGTVYVDIYSSRNLSDFIKLLTTAIINKFPERTPV
                     GKRFLKLLKGLRPVFSFDAITGAPQVQFNYQSESDKEYTLQQLLAFINEQPHPVVVAI
                     DEFQQIAHYPEKNVEAILRTYIQQFNRLCFIFSGSRRHTLMDIFANANRPFYSSTRFI
                     NLDAIERVTYRNFIREKFEQGGRAIDDESIDYVLEWTKGYTFYTQSLCNRLYSLRKIN
                     LDAVKAECLALLRENEPIYLQFRNLITSKQWEFLLALAKEESVSQIYSKEFLVKHQLG
                     TPSSIHRMLDALLEKEMILESVSSTGSSYSVYDVFLMRWMQRTY"
     gene            complement(33738..33965)
                     /locus_tag="PLW22_02970"
     CDS             complement(33738..33965)
                     /locus_tag="PLW22_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73697.1"
                     /translation="MKKIRTFNDEIERPERLCETCIYYIKNRTCVAYLDRIPEKYWKQ
                     KVGEKPLHDSPKDNPDYDNIFYKEKYIGKLE"
BASE COUNT        10491 a         7689 c         6921 g         9945 t
ORIGIN      
        1 tcttgtcttg atacaagaaa gaaccaaaga agatcaaggc tgaaaagccc gacccatttc
       61 tgggtcaggc ttttatgcct ctgcttgctt cttccgctcc attccctatc aggaatatgg
      121 gttagggtaa ggtcgtatcc cacgagcaac gaacaacgag caacttacac gaatgacttc
      181 atttgtcatc ctgagcatag cgaaggatct caaatcgacc ccatgagatg tttcgcttgc
      241 gctcaacatg acaatagctt cacgaacaac caacaacgct tccagccccg taggggcgta
      301 atatttgtaa caccatggtt attcccactt gcatagcgat gcacgcaata cattttccaa
      361 agagcaaagg tgaagctttg catcgcaaca gaatagcgtg gcaaagagca gtttccaaaa
      421 aaacaacaag gattaaaggc aaagagatgc ttcggctagc tcagcatgac agggaagcag
      481 aaaggaagat agggttttcg tcatcccaag caaagtcgag ggatctattc aaaggattga
      541 agagaaaaga gcaatgagat gcatcggtga agtctatccc gataaatcac gacagcgcga
      601 tgtttcgctt gcgcgaaata tgacaaataa ccttaactaa acaacattga aatatcaata
      661 taaaaacaaa aggggcttta cagccccatt ttgttacctt taacctaaaa ccaaacctaa
      721 acctaactca aaaaccaaaa gcctatgaga aaactaactc ttttctgttt caaatgacac
      781 tgcaaagata aaacccgcat tagctaatgc gggttaaagg aatattaagt ttagttaaac
      841 aatgttttat ccaccaaaat cgtcaaatgc aatcatctcc tcgggtacgc ctaaatcgaa
      901 taacattttt tgaacggcag cattcatggc tggtggacca cagaggtagt actcaatgtc
      961 ctcaggagca tcgtgttttg aaaggtaatt atcgtatata acctgatgga taaatcctac
     1021 atagcctgtc cagttatcct ctggtttggg ttctgaaagg gcaatggtga atttgaagtt
     1081 agggaattcc tgctcaattt tacggaagtg ctcctcgtag aaaatttctc gttttgaacg
     1141 ggctccgtac cagaaagtag ccttccgggt agtacgtttg gttaggaatt gatcaaatat
     1201 atgcgagcgc atgggagcca ttccagcacc accaccaata aacatcatct cattctgggt
     1261 gtcctttatg aagaactcgc catagggtcc tgaaatcata accttatcgc cgggtttacg
     1321 cgaaaaaatg aaacttgaac aaaccccggg gtttacgttc ataaaagcat ttttctccct
     1381 atcccagggc ggagtagcaa tacgaacatt gagcatgatt atattgtcct ccgctggatg
     1441 gtttgccatg gagtatgcgc ggaatgtttc ctcggtattc ttcatcttca aatcccacat
     1501 cttgtaccta tcccactcgt cgcggtattc aggttcaaca tcgatatcct tagcaaaatc
     1561 tacttcaatc ttaggtacat caatctggat atagccaccg gaacggaaat gtaacttttc
     1621 accctcgggt agcttaacca caaactcctt aataaaagtt gccacgttgt ggttcgatac
     1681 cacttcgcac tcccacttct taatacctaa aacctcctcg ggcacctcaa tctttatgtc
     1741 ctctctaatt tttacctgac aacccagtcg ccagtgatcg ttctgctgct tacgggtaaa
     1801 aaatcccacc tcggttggta aaatacttcc tccaccttcc aaaaccctgc acttacacat
     1861 gccacaagtt ccgcctccac cacatgccga tggaagaaaa actccgttat ttgatagggt
     1921 tgaaagaatt gttcccccgg gatctacttc aagagtcttc tcctcattaa ttaccaacct
     1981 caccctaccc tggggggtaa gctttgtctt agcgtatagc agaaccgcca ccagcaacaa
     2041 aataattagc aggaatactg caattgctga aattataatg gttccgttcc ctgaaagtaa
     2101 tatcatggtt aaatagtttt acagttttta caacttgatg cccataaagg tcatgaacgc
     2161 aatgcccatt aaacctgtaa gaatgaatgc aatacctaac ccgcgtaatg gtttaggaat
     2221 atcggaatat ttgagttttt cgcggattgc agcaatgcta acaattgcta gagcccaacc
     2281 tattcctgat ccaaccccaa aaacagtagc ctcggcaagg gtttcgtatt cacgttcctg
     2341 catgaataat gagcctccca gtattgcaca gtttacagca ataaggggta aaaatattcc
     2401 aagggagttg tacaacgatg gcgaaaactt ctctaccacc atctcaacta gttgcaccat
     2461 ggatgctata acagcaatga acataataaa cgagaggaag ctaagatcta aattcgcaaa
     2521 accgggatca agccagttta acgcacccgg tagtagtaag tacttgttca ttaaatagtt
     2581 tacgggaaca gtaatagcca tcacgaatat tactgcaatg ccaagcccca ttgacgtctt
     2641 tactgttttt gatacggcca ggtacgagca catacctagg aagtaagcaa aaaccatatt
     2701 atcgacaaat atggacttga caaatatgtt gattagatgt tccatacctt aggtcaattt
     2761 tttgtggtta atcctcaata agttttcggt tcctgctacg ttgtatccaa attatgatac
     2821 caacagtaat cagcgccatc ggaggtagaa tcatcatacc attattctca taaaaacctc
     2881 cgtttttgat ataccaatca atgggtataa ccctatagtt aaaaatggtt cctgagccaa
     2941 gtagctcacg gaaaaaagct acaataacca ggattaaacc ataacctaat ccgttaccaa
     3001 taccatcgag aaacgatggc cagggcttat tacctagtgc aaaagcctct agacgtccca
     3061 tgattatgca gtttgttata attaacccta caaaaaccga tagctgtttg cttacatcgt
     3121 aaacataagc tttcagtatt tggtcgacca gaattaccag cgctgcaata acaaccagct
     3181 gcacaataat acggatacgg ttaggaatcg aattccggat taaagaaatt atcaggtttg
     3241 agaaagcggt tactactgta acggaaatgg ccatcacaat ggcaggctta agctttacgg
     3301 taacagccaa ggctgaacaa atacccaata ctaaaacagt cactgggttt ttagagtcaa
     3361 ttggctcggt aagcagctta atgttctttg gggagaacca tggttccctt tccttaactt
     3421 cactcatggc taatactgtt ttttactttt tgaaaatagc tttggtaggc agcaaggcaa
     3481 tcgaaaagca tactttcgag ccctttgctg gtaatggtac cacccgaaat ggcatcaacc
     3541 ccatggggat cgttgggtgc ggcaccacct tttaccacct ttacggaagt aaaggttaaa
     3601 atgctgtcgc cctgaaaaat tgattttccc ttgaattgct cctgaaattc gtgagtggag
     3661 atttcagcgc ctaatcctgg cgtttcgccc tgatgggcaa aagtggcacc gtaaatggta
     3721 ttcaaatcat cgtttaaagc gatatagccc caaataggcc cccaaaggcc acgtccacga
     3781 aggggaatga tatactttat gctaccatca tcgagtttag ccttgtaaac cggaaggttt
     3841 cgctgctcaa tgggcttgga catttcaacc ttcaaatcta cattaaatgc aactacaccg
     3901 gttatggtgt cgccttttga gttaacaaca acctgctcag ggatatactt attgaagttt
     3961 tcagaaacaa atgcatgctt gttggcggct tgttcaaccc catcggcctt gcctattgag
     4021 cgaagtatat cgattttctt ttccgtttca atattcttgg tttgtgccgg ttttaaagcc
     4081 gttgaggcaa tagccagcag catggcaaca acaataacca ttaccgaaga atacataatt
     4141 gtataggcat tgctgtttcg gtccatgctt tttatcttat ccagtaaact catagctttc
     4201 aaagtttaga cgttagcaac ctttttggcg cgtttcattc ttcggcgaat gttggcttca
     4261 accacgtagt aatcaattag tggagcaaat gcattcataa gcaggatggc aagcatcata
     4321 ccttccgggt aagcggggtt aagcactctg acaagcaggg ccagaaaacc taccaaaaat
     4381 ccgtatatcc acttaccctt ctcggtttgc gatgcgctca cggggtcggt agccataaaa
     4441 acagcaccaa atgcaaagcc tcccataatt aggtgctgcc atgctggaat ctccatgtag
     4501 gggttaaccg caaaggcatt aaaaagtaaa cctgctgcca agccacctcc aaataccgaa
     4561 accatgattt tccaactgcc aattcctgtt actataagta tgatagcacc aataagaatt
     4621 gctaaagtag atgtttcacc aactgagcca ggaataaagc ccatgaacat atcgtatact
     4681 gaaggcaact ggtcaatttg gtaggctgct gctttggcaa gaggggtggc tcctgaaaaa
     4741 ccatccacta ctcctttccc ctgagtcagt ccggcaatcc aaaccttatc gcccgacatg
     4801 tgcgaagggt aggcaaagaa aaggaacgcc cttgccagca aagccgggtt gaatacgttg
     4861 taacctgttc ccccaaaaac ctccttacca attattactg caaaagcaac cgcaatggca
     4921 agcatccaga gaggaacgtc aacaggcatg attagcggga taagcaagcc gctaactaga
     4981 aatccctcat taacctcatg gcctctggac tgagcgaatg caaactcaat ggaaagtccc
     5041 actccatagc ttaccacaat taatggtaaa actttaagaa aaccatacca gaatgtctct
     5101 aaaaagcccc aacttgcacc aatggaaaga gcatgctggt aaccaacatt ccacattcca
     5161 aaaagcatgg caggcattaa tgcgataatc acaatggaca tagtgcgctt caagtccagc
     5221 gaatcgcgta tatggcttcc cttggtagta actgtattgg gcacaaacag aaaagtttca
     5281 aacgcatcaa aggttgagtg aaacatctca aacctcccac ctttctgaaa gtgcggctta
     5341 atcttatcca ggtaatttcg taacgctttc attacaatga tattattgtt agcttaactc
     5401 tttaatcatg gtttcaatgc ccttgcgtag aatagcctgc acctctgttt ttgaagggca
     5461 cacatactcg caaagcgcca tatcttcctc aacaacttcg taaattccta gttgctccat
     5521 cctatctata tcattagcca gaatggcttt aaccagatgc attgggtaaa tatccattgg
     5581 cataaccttc tcatactggc cggtaaccac cattgcccta tgaccaccat gcatattggt
     5641 atcgagggta aacctcttgc ctgggattaa tttcgataaa aaggtacttg aagcgctatg
     5701 cttattgaca cccggagcaa tccatccgaa aaactcatac ttattccctt cgggaataac
     5761 ggttaccatg ttatcataaa aacttaaata ccccaattta tcgacacgtg taccggtaag
     5821 cacatttcca cttatgatac gaacttgacc ctgtgtatta atattatcct taataatact
     5881 ctcaatgctt gcaccggaaa tcaacttata gtatcggggt ttcacaacct cggagccagc
     5941 taaggcaatt accttggttg catcgtagat acctttaagg aaaaggcgtc caataataat
     6001 aacatccaga ggatttacta cccatactac ctctccttta tttattgggt cgatatggtg
     6061 aatttgaaca ccaacattac cagtaggatg aggtccagta aaatagtgga actccactcc
     6121 ttttgcattg gcaaaagctt ttgcgggtgg aaaatcggaa cttaacccca agtgaacctt
     6181 cccggtggtt agcttacgaa tcgcatccac gcctgcctga aaactctcct cctcaccctg
     6241 aactacaaaa tctaaatcgg gagccagcgg tgaggtatca aagccagaaa tgaaaatcga
     6301 ttttggggaa tcagcaggat tggcaataat gccaaaaggc ctttgcctaa tgtaaggcca
     6361 agctcctgcc ttgagcaact ttgcaataac ctcatcgcgg gtaagcgttt cgggcgatgc
     6421 aggaccaaag tcctcataag ccacctcgcc actatcggcc tttacaacta cctccagtat
     6481 taccctgcgt tcaccacgcc taacatcaac cacttcaccg ctaacaggcg atgaaaatac
     6541 tatgttaggc ctatacttgt cgtgaaacaa tggcgaaccc gccctgacct tatcgccaac
     6601 cgcaacattg agcttgggta caagcccggg aaagtcggtt ggcttaaccc cgtaagtttc
     6661 agccttatcg aggcgaacca gcaccttctc agctgaaccc tttaacggga tattcaatcc
     6721 acgtttgatt ttaattggtt tagacattat tttagtgttt tgctaaaaca aatttaagga
     6781 attttgaact cctgtttaaa aaaactcagc cacaaatgta gtatctttga taacacgtta
     6841 acaaaaatat ttattgatgt atatcaaatt taccagatat atagatatta atatatctat
     6901 ttttatctta tacatattaa gccctctgct ttgttactca aataacagag aagtggctga
     6961 aaatgttttt ggaaaacctg aaaaattatc gaaccaatgg aaaacaaaca taaaatcggt
     7021 taaagcctac aaaaccggtt ttgagctaac tcaaccaata atagagttga ataccgacga
     7081 aactataact ttggaattcg acgacttatc agatgaaacg gagcaatacg aatacactgt
     7141 aattcattgc gactatagct ggaaccctac aaatatggtg ttcatggagt atgccgatgg
     7201 ttttgacttt aaccccattc acgattatcg gtattcttcc ggaacgttgg tgcaatatag
     7261 gcactatacc cttacaatac caaatagtaa ccttaagctt aaactttcag gtaattacat
     7321 cgtgcaagtt gtaaggcaaa gcaataggag cgaaattgtt cttcaacaac agtttagggt
     7381 atatgagcca ctggtaaaag ttgatgccct tattcgtcaa cccattgaac caagtattca
     7441 aagcacccac cagcaaatgg aactaacggt taatactgct ttacttggaa aggttgaccc
     7501 taataacgat gtggttgttc ttttaaacca aaacaaccaa ccctacaacc agctgacttt
     7561 taaaactcca catttcagcg atggccatat gttgatatac cacaccccaa catcacccat
     7621 atttgaaggg gggaatgagt tcaggcagtt gaacctaaaa tcgtttcatt atcaggttgc
     7681 cgaaatacaa ggcataaaac aatacgatgg tgtttaccat accatactta cgcccgataa
     7741 aagagtaaaa acataccgtt atagcgaaag cgctgatatt aacggtaaat tcattgttaa
     7801 atgcgatgat gttacagaaa gccaagagga agccgattac acatgggtat atttcaccct
     7861 cagcaacgac gttcagttcg atggcgatat ctatatattt ggcgagctaa gcggttggga
     7921 gcttaaccca aatttcaaat tgaaatataa tccggaatgc aactgctacg aaacccgatt
     7981 acttttaaaa caaggctttt ataactacaa gtatgtattt gtttcccaag gtttggctac
     8041 acctgacttt aaccgcattg aggcgaattt ttttgaaact gaaaacgctt acaatattct
     8101 tgtttactac aaatcgcaag gggcaaggta ttggcgatta gtagggctga atggagtgaa
     8161 cagccggtac aagaactaag attttattac aacaacagta aaggcatatt ttactaagcg
     8221 ccaattaggg ctttcctaca gaagtaaaat ttgcacttga gtaattttct ggttattcca
     8281 ctaaaaaaca ttagaatagg ttgttggttg gttgttattt gctttatgtg cttcaatatc
     8341 agccttttgt cgactttaat tgatttcggg taaaacaagg attaacttgc cattgccatt
     8401 aaaaaataac aaaccaacta aaccaaaacc tatgagaaac aaaattttaa gatttgccgt
     8461 tttggcaatt tttgcttcag caattgctgt aagctgttca aaggaagatg acgaattggt
     8521 tcagaaaccc aacgaaagtc aatggacagt tcgccccgat tttgctacgc tcgacacacg
     8581 tccacaggac attattgagc agttcaccct aacagagaag gatgctgatc ttgttaaagt
     8641 tgatgaaaac acccctaaag gtgcaaagta tgccttggtt attggtattt ccgattactc
     8701 cggaactcag tatgaccttc aatactgcga cgatgacgca accgactgga aaaacaggct
     8761 tgtaaaggaa ggatactccg ttaccattct tctggatcgc aatgcaacct cagccaatat
     8821 tcaatcggca attaacagtt tggttagtaa ggctgctgca ggtaccgaaa ttgctttttg
     8881 ctactcaggt cacggcagta acggtaatat tgttaccact gatctttact acataagcag
     8941 ctcatggttt aaaacaaaat tctcaacagc caagagcacc aagatgatgt tctgctttga
     9001 tgcatgccaa ataggtgcaa tgaaaaccgc ccttaaagca actggtagag ttgttgctgt
     9061 ggcctcaagc actactacat actcctacga tggcgatgct accatgaaga acggagtatt
     9121 tacctactac cagatgtatg gctttgacta taagggttac atttaccttg agcctgactg
     9181 cagctacgct tgcacgcaaa tgaaaaactg ggcatcggcc aggggactaa ctgttgcccc
     9241 atcgtatgtt gattcatact ccggtaattt cgatctttaa cccaatactt gtttctttaa
     9301 aaatttgagc cggagaaact ccggctcttt gcttttattc caaacggatt ggcaacgcta
     9361 aatcactaaa ccttcaacaa acccatcagg ggtaaacttt tctaaccgca cttttacaac
     9421 tttgcctgca tgccctttat cgaaaggaat acctacttta atatagttat cggtaaaacc
     9481 gaacatgtaa tcgcctttac gtgacgattc cacaaggaca gggcgtattt gaccaatgtg
     9541 tttcaggaaa aattccttat gtaacctatc ggatagtaca gttaaaagtg taaccctttg
     9601 cttaaccacc tttggactta ccttaccttc aaattcggtg gcaggggtat ttggacgctc
     9661 tgaataaggg aaaatatgta aaaatgatgg tttcacctgc tcaataaagc ggaaagcatc
     9721 gctaaaatcg tcatcggttt cgcctggaaa gccaacaatt acatctacac caaaaaaggt
     9781 gtcgggcata cggcttcgga ttagctcaat gcgttcggca aagagttcac ggcggtaacg
     9841 cctgcgcata agcgcaagta tcctatttga acccgattga agcggtatat ggaagtgggg
     9901 aaggaactta gccgattgtg ccacaaaatc gacaatttca ggggtaataa gatttggctc
     9961 aatggacgat attcggaagc gctcaattcc ctctacccta tcgagctgtt ggatgagttc
    10021 caggaatgtt tcgccagagg acttgccaaa atcgcctgta ttgacacctg taagaataat
    10081 ctctttaaca ttctgactgg ctatagcttt ggcctgatcc actatttgac taatgggcat
    10141 attgcggctt ttacctcgcg caaggggaat tgtgcagtag ctgcaatggt aatcgcaacc
    10201 atcctgaacc ttgaggaacg atcgtgttct atcgccagtt gagtatgccg ggaaaaaatc
    10261 acttaccgaa tcgatatcac agctataaac ttttactttt gtctttttag catcattccc
    10321 aatcagatga ggaaggttga acttatcagc tgctccaagc acaaagtcaa ccccatctat
    10381 ttgtgcaatt tcttcgggct ttagctgagc gtagcaaccc accacagcaa ccattgcgtt
    10441 gggcgatttg gttgtaaact ttcggattgc ctgacggcat ttcttatcgg catgctcggt
    10501 tactgaacag gtattaatta cataaacgtc ggcctccgaa tcgggcgaaa cacgttcgta
    10561 gcccatatcg gcaaactgac gggcaatggt agatgattct gaaaaattca acttgcaccc
    10621 cagcgtgtag aatgcaacac gctttctcac ctggttcatt gtaaaaaatt tttgcaaagg
    10681 taacaaatga aaaccattaa ggtttacacc aaatgaatat tagaatgtaa cagatggtgc
    10741 tattgctgca acgcaaggct ttgcttataa tcagcgtaaa gcgtacgaat aataccgtcg
    10801 gaaatcccta ttgtaggaac tatcacctct tttgcaccag tccatttcat caccttgaga
    10861 aagattttgg ttgccggtat aataacatct gcacggtcag ggtttaagct aagaaactta
    10921 attctgtcct caactgagaa actgtccaaa aaatcgtata aatttttcac ttccttttga
    10981 tataacggct ctcctattgg tttacgagca agcttgtaaa gcttattgat atttccacct
    11041 gacccaataa ttttaggttg gtctttcttt ttcactacac tcttaagcca gtcgcgtacc
    11101 tgatcaaaat cgggttttcc aatcattccc ttaagaatgc gaatagttcc aatattaaaa
    11161 gagtttgatg ccttgatttc acctttctca aaaacagtaa tttcagtgct accaccaccc
    11221 acatcaacat atatataagg tctatcatgg tcaagcatct ctgcaatacc attagcgtaa
    11281 attatttcgg cttcgcgtga tccatcaata atctctatgt taatgtttgc atgttgttta
    11341 accatactaa caagttcagg cgcattgctg ctcgaacgca ttgccgatgt ggcacatgcc
    11401 ctataggcaa ccacatcctg cgcttccatc aggtttttga aagcaatcat ggttttgagc
    11461 agtttctgag ctctgtgttc actgatataa ccaatagaaa aagcatcctc gcctaacctg
    11521 ataggaactc gcaccagcga ggacttgcta aaaactacag tttctccatc ctccaccacg
    11581 tttgtaagta gcaatcgggc tgcgtttgaa cctatgtcaa ttgcggcaaa ctttaatatc
    11641 ttcattttac ctctccgttc ttgtgccttt caatattttg ataaaagcta taaagttcca
    11701 gttgtgaacg aaactcgggt tcaccctctt tacagggctt aaactcattg caaccaccat
    11761 ggttaaccaa acgcgctttc acattgtcgc gaagcgcata gttaaccacc gttttcatct
    11821 cctcaattag gggtttactg taaataggag ttgccacttc aatgcggtag tcaaggttac
    11881 gggtcatcca gtcggccgat gaaatatagc atacttcact acccccattt gcaaatatga
    11941 aaatgcgaga gtgctccaaa aacctatcaa caattccata tacctcaata ttctcgctaa
    12001 tgccctttac gcctggcaca agtgcacatg ttccccttac cacaagttta atctttacac
    12061 ctgccttgct cgcctggtat agtttcttta tcatctcctc gtcaaccaga ttatttattt
    12121 ttccaataag gtaggcaggg agtcctttgg cagcattctc aatttcagta tcaattaatg
    12181 agtataagcg cctgcgcatg ttgaatggag caactagcaa atgcttgtat gaaaatgtct
    12241 tgtaatttgt ttcaagaaaa gtaaaaactt taagtatttc agaggttatt cttgaatcgg
    12301 cagtaaaaag gttaacatct gtatagatgt ttgcgttccc ctcatgaaaa ttaccggtgc
    12361 tcaccaccgc atacttacgt tccttgtttt tttcctgacg ggtaataagt gtcattttac
    12421 tatgcacctt taaaccaggc accccgaaaa ttacgttaat accggcttcc tgcatttgtt
    12481 ttgaccagta aatgttggca ttctcatcga aacgggcgcg taactcaact atggctgtaa
    12541 cttttttacc gttttgagca gcattgatta atgcatgcac aacacgcgat tcagtggcaa
    12601 ccctgtaaag ggtaatatga attgatttaa cagcaggatc aagggcagcc tcacgtaata
    12661 agcgaatgta atactggaag ctatggaaag gataatgaag catgaaatcg tgatcggaca
    12721 taatatccat tatgcgagta gcctcatcta attccttgac actaacgggc tcaagcggta
    12781 aatttataag gtgatttcgt cccaagctgg ggaatcggat aaaatccttg aagttgtgat
    12841 atcgccctcc cggaataaca taatcatcct cgtccaaatt aagccctttc acaatgaact
    12901 ttagcaagtc agtaggaatt ttttcatcgt aaacaagcct taccggttcg ccacgtttcc
    12961 tattcttaac tccctttgat atcttctcca aaaggctctc agaaatatca ttatccacat
    13021 cgagctctgc gtcgcgcgta atctttacag tgtatgcctc aaaagtatcg aaaggaagta
    13081 tcttgaatac cttggatagg caaagcctga ttacatcgtc gaggaggata ataaaacttt
    13141 tatcgccttc gttcggtaac tgtataaaac gctttacaaa cctcgaaggg atttcgatga
    13201 gtgcaaactc ctttgtattg ggtttttgac tgttcgtaag cttaaccgct aggtaaatgg
    13261 attgatcgac cagttcaggg aatttattgt tccgctttaa aattaccggt gccacaaatt
    13321 ccgaaatgtg atcgtcgaaa aaaccgttaa ggtaatcaat ttgcccagct gatagctgtg
    13381 tttcgttaat gagatagata ttctcctttc tgagctcatc aataatttcc tgaaatgtgt
    13441 tgtcgaactt tttctgatag tcaatgacta tccgttggat cttttcgaga attttacggg
    13501 gattatcgcc cataatcttt tttgccccct tttcaaccac caataaacgg cgtaaggtgg
    13561 caaccctcac ccggaaaaac tcatcgaggt tgttcgaaaa aattccaagg aaacgaatac
    13621 gttcaattaa cggtactgat ggatttgcgg cttcctgcaa cactctgcca ttaaatgtca
    13681 accagcttag ctcacggtta acaagaggta ttttttttgg tgccattcaa atctagtatt
    13741 tcgggcaata ttaattgatt aaccgtttac aggttaaaca ctgatgttaa gaaattgtta
    13801 aactaccaat caaagctctg aaaaacagaa tatacctttt cggcagagag ttctatccaa
    13861 ctgttaatgt caaacttaac cccaacaagt cccgatgttg gtagttcatg aatgcgattg
    13921 gctacaaaag tgtttaccaa ataggtaagt gttggattat gccctaccac cattacactt
    13981 ttgtattcat cgggtaatga tttaatcatc tccaccagct ctctttcatc ggcctggtaa
    14041 accattggct ctatccggat tttatcatgc ggaaaaccaa tggtttcggc aaaaagtatt
    14101 gctgtatgca ccgcacggtt agcagaacta gtaataatca gatctaaccc ttgcaccttg
    14161 ggtgcaatag cccttgcggt gttaacaatg tcgcttatac ctttggcttt taatgcccta
    14221 tccttatctg ctttatcggc ctcggcccaa gaggatttgg catgacggag tatgagcaac
    14281 gtttttgcca tttataatct ggtttaagtt aaagcaaatc gctagccagc tcagccaggt
    14341 agctacgttc gcccttaaca aggttaatat gggcaaatat ctcctggcca tgcattctat
    14401 cgcttaaata ggttaaccca ttcgatttca tatcgaggta agggtggtca atttgatgta
    14461 tatcgcctgt aaatacaaac ttagtaccct cacctgcccg ggttataatt gtcttaatct
    14521 cgtgtggagt gaggttttga gcctcgtcaa caatgaaaaa aacatttgaa agacttcgtc
    14581 ccctaatata tgccaatggt gtaattacaa gcttatcggc acgttgcatc tcttcaatat
    14641 gcatgtaatc tttgcttgtt ggtttaaacc tattttttat aacacctagg ttgtcgaata
    14701 ggggctgcat gtatggccct attttttcat caacatcacc cggtaaaaag ccaatatctt
    14761 tattggaaag cgcaacaatt ggtctggcaa gtagaatttg atcgaactgc tcttcctgtt
    14821 gtaatgctgc cgctagagca agtagggttt tgcctgttcc tgcttttccg gtaagcgcaa
    14881 ctagctgaat atcaggcctc aagagtgcat ccagggcaaa cgtttgctcc gcatttcttg
    14941 gatcaatgcc atatgctcta accttttcaa cacgatcaag ggtatcggtt gccttatcgt
    15001 aatgagcaag tgcactcgat ttgttccctt ttaaaatata gtactgatta gcgtaaggct
    15061 ttaactttaa atcctttgct aaaagtttct cctcggaatt gtaaagcttt tggattagtg
    15121 catcatcaac cttttcaatt atctcaatct ctttcttcag actatcaata tcttcaacct
    15181 tatcgttaag gtaatcctgt gcaacaatgc ccaacgattt ggccttcatt ctaaggttga
    15241 tatccttagt aacaagtata accggacgtt caggattatg cttaaccaaa tgaatggtta
    15301 tggctaaaat cctattatca ggcgtgaact cagcaaatga ttctttcatt tcagggggca
    15361 ttggcttacc agtttcaatt ctgagttttc ctagattttt ccctaagggc aagcccccgt
    15421 taaaaagctt atcgccggca attttatcta actccctaac aaactcccgt gcatggtaat
    15481 taatcatttc gttccccttc ttaaacttgt ctagttcctc aagtactact attggaataa
    15541 caatatcatt gtcctgaaat ttatacaaac actgaaagtc gtgaagaatg acattagtat
    15601 caatcacgaa aatcttattc actttactct ttgccatagt tttgcgattt aaaaattcat
    15661 ctaaatataa gaaaagctca atcaaaacaa tcattatcac cctaaatatt tgttaaccga
    15721 cagctaataa gttttgagcg agaaaatcac taaacttgca cccaaaacca agtaaaatga
    15781 cgtacaacga ggcacttgat tacatctact cacatctacc tatttttcaa agggttggga
    15841 aagcagcata caaagctgac ctagggaata cccttaaact tgatgagtat tttgggcatc
    15901 cacatcgcac ctttaaaacc attcatattg cagggacaaa tggaaaggga agcacatcgc
    15961 acatgctggc tgccgttctt caaaaagctg gatacagagt tgggttatac acctccccac
    16021 acctaaggga tttcagagaa cgaataaggg taaatggtga aatgattccc gaagatgagg
    16081 ttgctaagtt catttcacag cataaggatt tttttgatca aattatgccg tcattttttg
    16141 aaatgacagt tgcgctggca tttgattact tctcccgaat gaaccttgat gttgcagtta
    16201 ttgaggtggg tttaggaggt agactcgatt caacaaatat tatcaacccc attctaagtg
    16261 taattacaaa tattgggttt gatcacaccg acttgcttgg cgatacatta ccaaaaattg
    16321 catcagagaa agctggaatt attaaaccag atacaccagt tgttgtaagt aagtatcaac
    16381 ctgaggttga aaacgtcttt atagcaaagg caaaggaaat gaatgctcct atctattttg
    16441 ctgatcaaga aattcaggtt tacgaacagt atatagatac tacagggtta caggtattaa
    16501 aactagctga taaggttaga gatattaaaa ttgagattga cctgttaggc aattaccaac
    16561 gttacaatat ttctggggtt ctaaaatcaa tagaaatact aagcggtaaa gggtttttac
    16621 tctccaacga agcgctaatg gaaggtttaa ggaatgttca aacgcttacc gggcttatgg
    16681 gacgctggca aaaattatcg gagaatccac tcatctattg cgatacagga cacaatgtag
    16741 atggtattaa atacgttgtg gaacaactca ggcacattcc tcacaataac ttgcacatgg
    16801 taattggcgt agttgccgat aagaacattg atggtatgtt ggcactactg cctaaaaatg
    16861 caacctatta ctttacccaa gctgcaatac caagggcatt aaaccacgaa gaattaaaaa
    16921 agagggcggc agaacaccaa ctatacggtc actcatattc aacagtcgct ttggcattag
    16981 cggcagcaaa ggaagcctcc aatccaaacg atttaatttt tgtgggtgga agcacttttg
    17041 tagttgctga ggttgtatag tttttgattg ctgattatca atagaattaa agattttaaa
    17101 aattttttct ttaatttttt ccgaaaatat tttgcagatt tcaaactttc gcatactttt
    17161 gcatcgcatt tccaacgggg aatgaccaat aaaaaaagac caaatattgg gagtttagct
    17221 cagctggttc agagcatccc gcctcgcggg agggtcacag gctgagatan nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttcagagcat cccgcctcgc gggagggtca
    17401 caggctgaga taaaatattg ggagcttagc tcagctggtt cagagcatcc cgcctcgcgg
    17461 gagggtcaca ggctgagata aaatattggg agtttagctc agctggttca gagcatcccg
    17521 cctcgcggga gggtcacagg ctgagataaa atattgggag cttagctcag ctggttcaga
    17581 gcacctgcct tacaagcagg gggtcacagg ttcgatccct gtagctccca caaagccttc
    17641 gactttatcg aaggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntacag
    17761 caggcacgta agcgcgaacg ccagattaag gcctggaaga gccgtaaaag ggttgaggaa
    17821 ctgtgtgggt tcagtaagcc ctgaacccta cggttcagag catcccgcat ccggcgggag
    17881 ggtcacaggt tcgatccctg tagctcccac aaagccttcg actttatcga aggctttttt
    17941 tgtatgcact acacatacat catttactcc agttctaccg accgtttcta catcgggtct
    18001 acctccgatg agatttccca acgcatccgg cgccacaacg caccccacag ggggttcacc
    18061 tccatggcga acgattggag gctggtttac ctcgagcagt tcgactccat acagcaggca
    18121 cgtaagcgcg aacgccagat taaggcctgg aagagccgta aaagaatgaa tttattaatt
    18181 aggatttttg cttttttaaa aattaaagag cctcaaccac tatttcaagt tttacaccac
    18241 gggagccctt taccaacacc atggagtcct taattggatt ctgcttcaga tactcagcac
    18301 attcagaaga attatcaaaa taataaaaca taccgttaaa acttgaaaag cccttaccca
    18361 caagtaagat tttatcaaaa ttcaaagcgc tggcaagctc aacaattttt ttatgctcat
    18421 cgagactata ttcccctaac tctagcattt cacctagaat agcaaccttt tttgatgcat
    18481 gtaattttgc caaatttttt aaggcatttt ccatactagt tggattggcg ttatatgcat
    18541 ccataataac agtattgttc tgggttttta aaagttgtga tcgattattt gagggaatat
    18601 agctttcaat cgcatctttt atggtaccag ggccaactcc aaaatgtctt ccaacagcat
    18661 aagctgccat aacattctcc acattatatt ggcctacaag gttagtcaga atagttgtga
    18721 gtttacctcc ctcacttatt gaaatagaaa ggaaagggct ttcgggcttc tccacaacta
    18781 tgaaatcctc aaaatactta gagtatggaa taacgttatt tgctttatac ttatttaaaa
    18841 gttctacaag aataggattt tgatggttgt aaaatattgc cctatctctc aaggctaaaa
    18901 agttataaag ttcccccttt gtatttttta caccttcaaa accgccaaaa ccaccaaggt
    18961 gagctttccc aatattggta atcaaaccga aatcaggttc agcaattctg caaagttcgc
    19021 taatatcacc tggatgattt gcacccattt cgataatcgc aaaatcaaca tctggagtaa
    19081 tggatagtaa cgttaaggga accccaatgt ggttattcaa attaccaacc gttgcataag
    19141 ttttaaattt ttgagaaagg acagtgtaca ataattcttt ggtagtggtt tttccattgg
    19201 aacctgttat agcaaaaatt tgtgcattga ttttttgcct atgataattt gcaagttgct
    19261 gaagtgcatt taaggcattc tccaccaaca cacacccctt ggtcatgtaa tggggattat
    19321 caattacagc aagggctgct ccatcctgca aagcattggc agcataggca ttcccatcgt
    19381 tattttcacc ctttaaagca aaaaatattg agccagcttt aacctttcgg ctatcgatag
    19441 ttacgtgata ggatttttca aattctttat aaatatgttc aatcataaca aaccagtaaa
    19501 aaagaaaacc tcataaagat atgaggtttt ccaaaaatat tgatttatat tgactatttt
    19561 cctccaattc tctcaccacg tttctttgcc gattgagcac caacggcttc cattgcgcaa
    19621 cggaacccaa tatcattggt tgatgatgct tcgtcgagga aacggcgggt acctgggctt
    19681 aaccagtatg ccctatcctt ccaagaacca cccttataaa ccctaacatg gtcgttaatc
    19741 agagttgtca tacgcacatt cttctgttga gtatcgccct gatagtacat cctgtcggag
    19801 tttttctctt tcacagtttg tccatctctg aaatcaagcg ttgaggcaac atcaccatcc
    19861 ttatagttga tatagtaggc tttattgtag ttctgtcggt tagctgcttc ttcctcggta
    19921 acttcacggt agggtatgcg acctaaacta tccttcttgg agtagttacc gtctaaatca
    19981 tatggggttt tgaaaacatt accacggaac ggacgaattt catcaacatc ttcaaaactc
    20041 aaggggcggt aaacgtcaag cacccattca ttaacattac cagccataca gtataggccg
    20101 taatcattag gccagtatga tttaactggt ccagggaatg tgttctcgtc gttcaaatta
    20161 cctgcaagcc ccatatagtc accacgaccg cgaacaaagt tagccatcat ctggccacga
    20221 acttttgact caggattacg cacgttgtgc ccattccaag ggtaaatacg acgctcaacc
    20281 atgcgctcgt cataggtatt acctacaagt cccgatgcgg caaactccca ttcagcctcg
    20341 gttgggaggc gatacttggg tagcaggata ccatcttcaa agcgaacgtg acgtccttcg
    20401 ggtttttcag ggtcaaggct gggtaaaagc ttcttaacgc gcgcatcgta ctggcccgaa
    20461 aggtaagcct cagtgctaaa tgcatcagca cctttttgag ccaggtcaaa ttcaataatg
    20521 cccttatcaa ctaaaagacg ctcatttacc ctatcggtac gccatataca gtaatcgttt
    20581 gcctgaagcc agttaactcc tactacagga taatcgttat aggcaggatg cctgaagtag
    20641 tcggttacaa aaggttcatt gtaaccaagg ggcgaacgcc atacaagagt atcgggtagg
    20701 gcatttttaa ttacctgtgg ataggttgaa tatacacgtt taatccagtg aacatattca
    20761 cgccaatcta cattgctaat ttcggtttcg tccatgtaga atgatgtaac tgttaccctg
    20821 cggggtttag cattccagtc gtacataaca tcctgctcta ctcgtcccat agtaaatgta
    20881 ccaccctcaa tgaacaccaa gccgggacct gcctcgggtc gccagttttc aactacctca
    20941 aagcccccat tatcagggct gttatactcc cagccagttt tttcggacgc tccagttccg
    21001 ccaccaccac caccaaataa accgcaagag ttaagaccaa aaattccggc tgatgccaat
    21061 aataagattc tatactttaa gctcatagct gtacggattt tctcattata attaaactca
    21121 aatatataaa taatattttg taatgcgccc taaaagttta aaaaaaaatt aaaattttgg
    21181 acatttgatg taccggtatt tctttttccc ccttttatac tcaaaattcc accctaaggt
    21241 tacctcgtgc gatgaggttg gtaaccctct aagaccatgt tgcataatac tgaaatcgta
    21301 actgtaacca atacgaaaca tataatcggc atatcccaag gtaacaacaa acgaatggct
    21361 atttaaactt aaattttccc tgaaccagag tcctgcaact gcccagttct ttgaaaggta
    21421 aaagccaagg tttagctgat taaaatttag ctgttgaata tagattacgt ttggagaaaa
    21481 aagcagaacc tccttaaacc ttctccactt atataggtta aaatcggcac caatatggaa
    21541 agtatactta cggggaatgt atgcttttcc gcccgagaaa ctggcctcaa caggttgtaa
    21601 aagatgatgc accgcaaatc caccataaaa attttcaaaa tcaccggcaa caccaagccc
    21661 tatatcgtaa ttccaggttg tatagcctac ggcgtcggaa tttccaatca cattaccagc
    21721 cggatcaatc atatccggga aaaccaaacc tgaaaaactc cgtgtacgaa aaatagctga
    21781 ggtttgaagt ccaaatcgta cgtgtgagtt atatgtaggt tgaattccgt atgagtaaat
    21841 aagatcgaga gtgttccggg agaatgttcc tttcccttcc acatcgctca taatgtttat
    21901 accaaagccg ctccgccctg aggattgaga acgattgtta cgaaacgagt gtttccagct
    21961 tgggccaaaa tcaatcctcc tgtcaaaact taccccaaag gttgagtatg gcatatctat
    22021 catggtccac tgattgcgat atataatgcc taatcgcata tcctttgatg cgcctgcata
    22081 cgatgggttg agcatggttg gattaaaaaa aacctgtgaa aacacaggat cctgaccatg
    22141 tgatttttca aaacatagga ctaagcccag caagtacaat aaaaaaaaac tgtttttaga
    22201 tatcatttaa ctggtttgta ataataacta acattattcg ttttttattg ttagaatcaa
    22261 acgtaaaggt aaagtaaagt tttgtttctt tgcaacacat cagtcaatta tgagggcagt
    22321 ttttttcatc atattattaa caacacaagc attgggggct tttacacagg agttccacag
    22381 aattcttgaa tgggttgaaa ccgacaaaaa aaatatcaac tgtttatggt ttaatggcga
    22441 agattttcct gacccagcca gtaaggtccc atactatacc gaattaatac caattcccga
    22501 tagtaaggag tacagaattg aaataacaca tataaaatgg ggtaaactca atagagattt
    22561 tgattctagt attgttcaaa ctgaaccaaa tccaacaaca aaaagcttga gggttggggc
    22621 aaacagggtt ctgctttttt caatacccgc aatgaggaac aatgcctctg gctcaattga
    22681 gaaacttgaa tcgtttagct tcagaattga gcccatcagt tcccatgaaa aaaatattga
    22741 taaaacgatt cgtacctcgc tttcgaactc ggttcttgct tcaggtaaat gggtaaaagt
    22801 gggcgtgcaa aaaagtggga tttataggat ttcattcgaa gatttacaaa ggtatggttt
    22861 tagcaaccca gaaaatatta gcatttgggg aatcggagga aagtcgttac cccttatgaa
    22921 tgcccaaagc aacctcgatg aggtagctcc aataccaata caaataagta aaggtgccga
    22981 cggcattttt aatcagggcg actatattct attctacgct cagggccccg aaactttaac
    23041 gtataatgaa acatatagtg catggaaatg ggaaaagcat ggatacacca aaaccattta
    23101 ctacttttta acaaccagtc aacctcaaag catcatcggt tttgctgaca cgccaaccga
    23161 aaccgaaaat tatgcaacaa atgagttcga tcaagtaaca acctttgaaa gcaacgatac
    23221 aaaccttgtt aaatcgggca gggaatggtt tggcgaaatt tttgacataa aaaccaccta
    23281 cagctacaat gtatcaacac cactcccagt aaacaactcc acctataaag tatgggtaag
    23341 agtagcggca cgatcttcgt caacctcaac ttttaccctt acaaataatg gaacacttgg
    23401 tagcataact ttaaattccg taataattgg cgatgagcta gccaatgtgg tttctgtaaa
    23461 cgaaaaagta ttctcgggta atactgttgg acaaaaccta acatttgacc ttacctacaa
    23521 taaacccaat tcgggttcag tgggtttcct ggactttata tcgattcaga gccgacatgg
    23581 cttattgtat agtaatgccc aactgccctt tcaggatttt cagtcggtag gggcagggag
    23641 aattagcagg ttcacaatca gcaatgcaaa cagttccgtt caggtttggg atattactga
    23701 tatcaatgag gccaaaaggg ttaatatttc ctacgccaat ggcacaataa cattcaagca
    23761 agctaccaat actctcaaaa cctttattgc ctttgagccc gataaagcct acagtgttgt
    23821 agggtatgca aatgttgata accagaatat tcgtggaagt agcttcttcg attactttat
    23881 agttactcat ccaagtttta aaacctatgc cgaagagctg gctcagctcc accggcaaag
    23941 aagcaacctc tctgttgctg tatatactac cgaccaggtt tacaacgagt tctcgggagg
    24001 taaccccgat gttgcagcaa ttcgcaatat gctacgttac ttttataaag ccgcaaccag
    24061 cgacgatgat aaacctaaat acctactcct attcggcgat ggctcattcg ataacctttc
    24121 caacagccaa gtcaatacta attttattcc cacataccag tctcctcgct caataaacag
    24181 ggttgagtca tttgtatcgg acgatttctt tggattactc gatgataatg aaggcgaagc
    24241 ctcaggttac attgacattg gaattggtag gctacctgtt agttcaccgg aacaagccgc
    24301 caatttaatt accaaaatta agagttacat ggagaatgaa gatattgacg actggcaaag
    24361 caaattggtt ttcataggcg acgacgagga tggtaacgtt catatgcagg atgccaacac
    24421 gctggcaaag tacattgaaa ccaatcagcc ggcatataat atcctgaaaa tatttttcga
    24481 tgcttaccaa caggaggttg tttcgggtgg tcaacgctac cccgatgtta ccaatgccat
    24541 caattctgct gttaacaatg gcgctttact tttaaactat actggtcatg ggaatgaaag
    24601 gtggcttgcc cacgagaagg ttttaatgct aaacgatgtg ttgggatgga aaaacgctaa
    24661 acgcctacct ttatttgtaa cagcaacctg cgagtttagc cgttttgacg actaccacat
    24721 gacctcaacc ggggaatgga tactacttac cccaaagggt ggtgcaatag cgttactctc
    24781 tactacccgg cttgtttact caagcccaaa cttcacttta aactacaact ttatacagca
    24841 gctttttaaa accgattcca atggcaacta tctcaggctt ggtgatttgg ttcgcataac
    24901 caaaatacta tcgggaaccg gttacaataa acgcaacttc accctgttag gcgatcccgc
    24961 attgatgcta aaatacccta catacaacat aaaaatctca tatattaatg aacgcccagc
    25021 atcagagcca gctgatactt taaaggcact taataaggta aaccttaaag ggtttattgc
    25081 cgataacctt gacacaactg ttgaaaattt caatggaacc gtttcggtta ccctatacga
    25141 taaggaacgc gaaattacca ccctggctaa cgacggcggc actccgatga cctttaaaag
    25201 tcgcgataat atcatattta aagggaaagc aactgtttca cagggtactt ttagtatcga
    25261 ttttacggta ccaaaagata ttaacttctc atacgggcat ggaaaaataa gtctttttgc
    25321 aaccgatggc atgcaaacag ccctaggcgt acacgattca atagttgttg ggggaattgg
    25381 taaccctgaa agcattgata gtgaaggccc tcaaattaaa atctacctga acgataccaa
    25441 gttcactaac ggtggtattt gcaactccaa ccctctgcta ctggtttacc tttccgatgc
    25501 gagtggcata aacacaaccg gagcagggat agggcatgat cttaccgcaa cattaacata
    25561 tccagccgga caaagcgaaa cattcaacct taataatttc tacaaagcca ataccgacga
    25621 ttacacccag ggtatagcaa cctatcagtt ctccggccta actcccggaa cttacactat
    25681 agaggtaaag gcctgggatg cctttaataa tagcaatacc ggcaaaatag ttttccgggt
    25741 aattggcgac gataaacttg tggttggtaa cttttactgt gttccaagcc cagttaagga
    25801 taacacacag ttctactttg aaacaaacac caatgaaact ggacttgatg taaagctgga
    25861 aatattcaac atcaatggct ctaaagttgc cgaactccta aaaaaaagga tttatactga
    25921 aggttacaga atagggccgc ttgaatggaa cggatgtaac tccaacgggg agaaacttgc
    25981 aaagggaatt tactttgctc gcattcaagt ttcatcggta cagggtgagc aggtagccac
    26041 aacaaaattc gttgtacttt agtttgatat taaacaataa gtatcatatt acaccgttta
    26101 catttgttac tgtataaatg ttatttttgc aaactcataa ttgttaagtt gatgattaag
    26161 aaattttttg gaattgcaac aattctttta gtaggattac agcctactgt taaggctcag
    26221 ttcgatagta ccatatcgct atcgggtggt ttaaatgcat tgcgcattgc tgtaccattc
    26281 ctaaccatag cacccgattc acggagtgca gcccttggcg atgccggagt ggcatcggta
    26341 cccgatgtaa actcgcaaca ctggaaccct gcaaagtttc catttattga aggtaaaaag
    26401 tggggcatgg gattttccta caccccatgg ctcagaaact tagtaaacga tatcaacctt
    26461 acccatttaa ccggttatta ccagtttgat aagatgcaat ccattagcgg atcgttgctt
    26521 tacttctcaa tgggtgaaat caccttcagg gataacaata acaactacat ccaggccttt
    26581 aaccctaacg agtttgcaat cgattttgca tactcgcgta agttttccga ccatattgcc
    26641 ggtgcaatgg ctttccgtta catccgttcc gatttaaccg gtaattttac taccacaggc
    26701 actggcagcg gtaaacccgg tacctcgttt gcagccgatt tggcagtata ctaccagaac
    26761 gctattaacc ttgccgggaa aagtggcgaa atggcaattg gtggcaatat ctccaatatt
    26821 ggaacaaaat tatcgtacaa tgaggatcca caaaaggagt ttatacccat taacctaagg
    26881 gttggtggaa ggttatcgct taacatcgac caatacaatg ggttttcgta tctactggat
    26941 ttaaacaagc tgttagttcc tactccacct gtttacaaca gccaggatga gattgttagc
    27001 ggaatggatc caaacgtatc ggttgtgcaa ggtatgattc aatcgtttta cgacgcccca
    27061 ggtggtttca aggaggaaat gcaggaaata tacatagcca ctggggttga gtactggtac
    27121 gcaaggcagt ttgccattag ggcaggatac ttccacgaga gccaaaataa ggggaaccgt
    27181 aagtatatca cccttggggc tggattgcgt tacaatgtgt ttaatatcga cttctcgtac
    27241 ctaatcccaa ccggtgggtt caatagcccc atggccaata cgctacgctt ttcaatctct
    27301 tttgaatttg aacctgcaag aacaagaaca aatagagata agtaatggat tttaggatag
    27361 gttttggcta cgatgttcat cagctgcagc aagggctccc tttttggtta ggtggagttc
    27421 aaattgagca caccaagggc tcattaggtc actccgatgg cgatgccctt atccatgcca
    27481 tttgcgatgc catgctgggt gcagccaacc tgggcgatat aggtgtctat ttccccgata
    27541 ctgatccctc tttaaagggt atcgatagta aaatactttt acggaaaaca atggatataa
    27601 ttgcccagaa aggatacacc attggaaaca tcgactcaac catctgccta caacgcccaa
    27661 aggtaaagga ttatataccc aaaatgcagg ctgagcttgc caaagcatta gatatagagc
    27721 cacagcaaat atcaataaaa gctactacta ccgagaaact tggttttgtt ggacgcgagg
    27781 aaggtgtttc ggcctatgcc gtagttcttc tgagaaagaa ttagctgtat ggaggcagga
    27841 aacgatattc ttccaaaaac cttagtacta ggcgctagtc aaaaacctga gcgttactcg
    27901 tacctagcca tacgaagcct taggaaacat ggtattgagg tagtagcaat cggtgcacgt
    27961 aaagggatgg ttgatgatat tgagattcaa accgaactac aggatatacc agatattcac
    28021 accgtaacac tttatctcag cgcacgtaat caggctccat actacaacta tatcatcaac
    28081 ctcaaaccca aacgtctgat ttttaacccg ggaactgaaa accctgagct ggaaaaactt
    28141 gcccgagaac atggaattga cgtggtggag gggtgcaccc ttgtaatgct aagtaacgga
    28201 acatactacg aatggtaaaa gttatacaga acgagtccga tttaagtaag gccctgagcg
    28261 agccagccag tgctatctac tttaactcac aggcatgcgg ggtttgtcat gttatgctgc
    28321 cccaactcga gcaaatagtt ggtagtgaat tccccaagat gcacatttac gtggtaaatg
    28381 ccaacgaaac tcctgagata tgcgggaaat tctcagtatt tacctttcca accgtgttaa
    28441 tattttttga agggaaagaa tcgttgcgta tggcaaggaa tatcaacctg gccactttca
    28501 ggcaagccgt agctcgcccc tactccatcc tcttcgacta gggacgatcg aatgttgcgc
    28561 taataaatgg gtcgtttacc tgccctgaaa cactatactc ccagtcaatt gtttggctgt
    28621 acctgtgcca aacacccctg ccagttataa agtatagatc gctattggtg cttagcaccg
    28681 taaaaacggt atccttaagc tgaacatagg tttcaacgtc aagcccaagg ttgtaaaggt
    28741 tataaatcac gtaggttgag ctatcgagtt gtgtgtagcg gtcaacactc acgttatacg
    28801 acctgtattt ggttccctgg taattctccc ggcaccgcca aaccccggta atcccctccc
    28861 ccttttcgta cttgtcgcac gatgaaccag atagcaataa aaaagttgct atgaacaata
    28921 acagttgggc agtccttttc ataactcaag tatataagtg atgaagggca ttagcccttc
    28981 accaccttat caattaaact atcgtcggca atattaggaa gcgtaacctt gagcagtggg
    29041 gtgcgttcca tctcgcgctt aatggcaagc attgcgccgc tgttacgcgc ccaactccta
    29101 cgggcaatgc cattgttcac atcccagtgg agcatcatgt ggaggcgtct atcggcagca
    29161 tcggacccat cgagagtgag gccaaagcct ccgtttatta cctcgcccca gccaacacca
    29221 cctccgttgt gaattgatac ccaggtggcg ccacggaaag catcaccaat tacgttgtgg
    29281 atagccatat cggcagtgaa gcgtgagcca tcgtagatgt ttgatgtttc gcggtatggc
    29341 gagtcagttc ccgatacatc gtggtggtcg cgacccagca ctaccggggc tgaaatgcgc
    29401 ccgtcgcgga ttgccttgtt aaaggcagca gcaatcttaa tacggccctc ggcatcggcg
    29461 taaaggattc gggcctgtga tccaaccacc aggttgttct tactagcctc acgtatccag
    29521 tgaatgttat cctgtagctg ccccatgatt tcggccggag cagtcttggc tatctcctca
    29581 agtacctcaa gggctatagc atcggtggtg gccaaatcct tgggatcgga cgaggtgcaa
    29641 acccaacgga atgggccaaa gccgtagtcg aagaacatgg gtcccattat gtcctgaaca
    29701 taggacgggt acctgaaatc gccgttaggt ttaaggatat cggcaccggc gcggctgctc
    29761 tccagtagga atgcattgcc atagtcgaaa aagtacatgc cacgggcagc catggtattt
    29821 atggccttta catggcgacg taaactctca taaacctttt ccttgaaaag ctctggctgt
    29881 tcagccatca tcctgtttgc ctcctcaaag ctaaggccca ctgggtagta ccctccggca
    29941 aagggattat gtaatgaggt ttgatcgctt cccaaatcaa cgtggatatt ttcggtaacc
    30001 agacgctccc agaggtcaac aatgttaccc tggtatgcca gcgaaacggc ctccttattg
    30061 gccgacgcct gacggatacg agggatgagt tcatcgaggc tggtgtaaac ctcatccacc
    30121 cacttttggc catgccgaac ctcaacggcc ttggggttaa tctcagcaat aacgccaacc
    30181 attcctgcaa ccacggcagc cttgggctga gcacccgaca ttccaccaag acctgatgat
    30241 acaaatattt taccccgcaa atcggtttcg cccggtttca tctgtttacg accggcgttc
    30301 atcacggtaa tggttgttcc atgcacaatt ccttgagggc caatatacat gaatgaaccg
    30361 gctgtcatct gcccgtattg ggtcacgcca agtgcgttgt aacgctccca gtcgtcctta
    30421 ctggagtagt tgggaatcat cattccatta gttaccacca cgcggggtgc atcggggtgc
    30481 gatgggaaaa gccccatggg atggccagag taaagaacaa gggtttgctc atcggtcatc
    30541 tcggccaggt acttcatggt taagcggtac tgcgcccagt tctggaacac agccccattc
    30601 ccgccgtagg ttataagctc atgcggatgc tgcgccacag cataatccag gttattactt
    30661 agcattagca tgatagatgc agcctgaacc gatttatgcg gaaaatcgtt aatggatcgt
    30721 gcataaatct tataatcggg ccggaaacgg tacatgtaga tacgaccgta ctcttccagc
    30781 tcacgggcaa actcctcggc caaaacagca tgatgctttg gcggaaaata cctaagggca
    30841 ttcttaaccg ctagcttctt ctcctcaagg gtaagaatct ttttgcgctt aggcgcatgg
    30901 ttaaccgagg gatcccatgg cttgggtgct ggaagctcat cgggaatccc ctgcagtata
    30961 tccttacgga actcttcaag tgtcatagtt acattttttt actcttaccg caaaggtagg
    31021 aattttaaaa cgatttcagg cgagggaggt gggaaagatt attaagatgt aaaaggagtt
    31081 agaagaagtt agaagaagtt agaggaagtt agaggaagtt aaaggaatgg ggtggaatga
    31141 accggaatga accggaatga accggaatta ttcggaatta ttcggaatgg gagggaacca
    31201 gttgtaacag ggcggtattg ctgtattaaa aaaaactaac gtttaatcca ttgatgtaaa
    31261 caaatgtttt ttataaattg cattacttta gtaatacttt acctattgca atagcttaaa
    31321 gttccaaatg caacgatatt aaatacaacc caaaattatc acttttcaaa atggaataga
    31381 tagaataaag atgaagatat gagcacaatt gcaataaccg actgcgaagg caacgttttg
    31441 tatagccttg aagaatgtga gatgttcccc aattacactg cagtactaaa agatgcaatt
    31501 gcacagggta aagacctaac gggattgtgc attaaaaacc aaaacataag taatgtagta
    31561 ttccaaggaa ccacgcttta caatgcggtg ttcgagaatt gtaactttca taaggtcgag
    31621 ttcattgaat gccaagccga ttccgtttgg ttcaatagat gcaaccttga tgggcttaaa
    31681 gtcaaaaaga caaacctttc caaagcccaa attcacaaat gtagtattag caaggcacac
    31741 tttaccaggt caaatcttaa cgactcttta atatttgata gtaatttaca ccttgccgtt
    31801 ttccttaaga cacaactcaa taatgttggg tttgataatt gcaacctaga aaaagcaaag
    31861 tttgaagagt gttcactggc tgaagcagga ttttacaatt tatctccaac ctaccaatgg
    31921 attaacgatg tcctattctc aagatgcata ttgaataact gtaacctgag ttcgctgaac
    31981 tccctctcga tgcttttcat tcgggaaagc aatattgaag atgcagaact tcaggatcaa
    32041 aactttacca gggtaatcaa tacttacact aaagtgctgt atgccattga atccgatacg
    32101 gtttggtggg aagactttag aggaaacttt gagcagttta aagatatggt tgagaacgac
    32161 acgatggaaa acggaactaa atattacctt gatgactttt tgtatgacga gttaagcaag
    32221 gttgtgtatt acctggagca gtggcataat gaacaaacaa ttattgctca aaatacgcta
    32281 agcatcaata aatgcgaata gcaattgcac taaacacgat tggttcagat tgtatcgttt
    32341 gcatattggt catacccttt gctacaccgc ataaagtgct acagacatgt taatatgttc
    32401 tttgcatcca acgcattaaa aatacatcat acacgctata gcttgatccc gttgagctaa
    32461 cgctttcaag tatcatttct ttctcaagga gggcatcgag catcctatga atggacgagg
    32521 gtgtacccag ctgatgcttg acaaggaatt ccttgctata tatttgtgat actgattcct
    32581 cctttgccag ggcaagtaga aactcccatt gcttactggt gattaaattt ctaaactgaa
    32641 ggtatatggg ctcattctcc ctaagcaacg ctaaacactc tgccttcacg gcatccaagt
    32701 taatttttct caacgaatat agcctattgc aaagactctg cgtgtaaaat gtatagccct
    32761 ttgtccattc cagcacgtaa tcgatgcttt catcatctat agctcttccc ccttgttcaa
    32821 atttttcacg aatgaaattt ctgtacgtta ccctttcaat agcatcaagg ttgatgaaac
    32881 gggtactgga gtagaaaggt ctattggcat tggcaaaaat atccataagg gtatgccttc
    32941 gactaccact aaaaataaaa caaagccggt taaattgttg aatatacgta cggagtatag
    33001 cctccacatt tttctcgggg taatgagcaa tttgctgaaa ctcgtcaatg gcaaccacaa
    33061 caggatgagg ttgttcattt ataaaagcaa gcaattgctg gagcgtgtat tccttatcgc
    33121 tctcggattg gtaattaaac tgcacttgcg gtgcgccagt gatggcatcg aacgagaata
    33181 ctggtcgtaa gcccttaagc agttttagga aacgctttcc cacaggggtt ctctcgggaa
    33241 acttattgat aattgcagtg gtaagcagtt taatgaaatc agagagattg cgtgaggagt
    33301 aaatatccac gtaaacagtt ccgtaatcac ccatttcgcc aagcatctca aaaaagcgta
    33361 aaattagccc tgttttcccc aatctgcgtg gcgatatcaa agttgtattc acattacccc
    33421 ttacattcct cagcaggaca ttcaactcat tttcacgatt gcaaaaatac tcctttgaga
    33481 tataactatt aaccaagaag ggatttatat cctttaccct agccatttta ccatttattt
    33541 tatgctaata tatgtagaaa aacacatttc gcaaaatata taacgcattt tgtataacgc
    33601 atattgcgaa attgatgtat ttaataatca aagtttatgg gttaaccaaa cttattcaat
    33661 ccacgcaccc gcgcggggtg cgactaatag tttagcggct ctggcgccgt agtcccctaa
    33721 cttgtcaaac cctttcatta ctctagtttt ccaatgtatt tttccttgta aaatatgtta
    33781 tcatagtcgg gattgtcctt agggctgtca tgcaacggct tttctccaac cttttgcttc
    33841 caatactttt cgggtatgcg gtcaaggtaa gcaacacaag ttcggttttt aatgtaatag
    33901 atgcacgttt cgcaaagcct ttcggggcgt tcgatttcgt cgttaaatgt tctaattttt
    33961 ttcatggctc tattttttta ggtaaggatt gtctaattta agtaacattt ctacaatttc
    34021 aatcatttct attgttaatt gcatatgctt agccgaactt gcaaaaaaca ctcggttaag
    34081 attgtatctt tgacatactg gtcacaacat ttcaaccaat tttttgtagg tttgtggtga
    34141 tgtgaattgc tttggcttcg ccgagctcgc aaaaaaagtt cggttcagat tgtatctttg
    34201 acatattggt cataactttt cttatcttga caaccacttt ggtaaaggtt cttacgttgc
    34261 cattaagggt tttgcccaac cgtttgactt ttacctggct gatgaaaagg actcaattta
    34321 tattgatacc tatgagcctt atgaactaca agaccttaat acacttctct actataaggt
    34381 ttatcaaatt gtataactaa ccactaaaac tttatactat gaaacaggaa atggcttttt
    34441 acttttctgc tgcttctttc atcctttacg ctttaattct tctatttgct ttttataggc
    34501 atgtagatag attaaaaaag ggttacgagg ttagaaattt tatgggggta ctaaaatggt
    34561 tgtttttatt cactttaatt tacttcttta tttgtgagat tattttaaag gatgttttat
    34621 ttcatttctt ttaactctta caattaactt aacaatcgga gaggtcggcg ggggttgaac
    34681 tgttgatttc ttgttgtgaa ttgctttggc ttcgccgagc tcgcaaaaac gctcggttca
    34741 gattgtatct ttgacatatt ggtcacaaca gatatgttgt tttattctgt aaatcaattg
    34801 ttgtgaattg ctttcagatt gtatctttga catattggtc acaacttttg tgcccctatc
    34861 cccacggggg aaatagttgt gaattgcttt cagattgtat ctttgacata ttggtcacaa
    34921 cttgatcgca gtaaggttta atcttgctaa tgttgtgaat tgctttcaga ttgtatcttt
    34981 gacatattgg tcacaactca tcatcttcgt ttagatatga tgatatgttg tgaattgctt
    35041 tcagattgta tctttgacat attggtcaca acgactgggc aatttttaaa aatacattac
    35101 acgttgtgaa ttgctttcag attgtatctt tgacatattg gtcacaactt ttaacaaaaa
    35161 tatcgaaatc atccctatgt tgtgaattgc tttcagattg tatctttgac atattggtca
    35221 caacagatat gttgttttat tctgta
//
LOCUS       DAOOWJ010000016        33043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1373_length_33043_cov_7.748848, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000016 DAOOWJ010000000
VERSION     DAOOWJ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 33043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33043
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1373_length_33043_cov_7.748848"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLW22_02975"
     CDS             complement(<1..282)
                     /locus_tag="PLW22_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOU73698.1"
                     /translation="MVVDKISDLKTMLQGFRSEGKSIGFVPTMGALHKGHVSLVERSV
                     AENDITVVSIFVNPTQFNDKNDLKNYPRMPEKDIAMLEAAGVNVVFMPTE"
     gene            585..1424
                     /locus_tag="PLW22_02980"
     CDS             585..1424
                     /locus_tag="PLW22_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOU73699.1"
                     /translation="MKEKKAKILFISQEILPYLPESEVSKISRFLPQGIQEKGREIRT
                     FMPRYGCVNERRNQLHEVIRLSGMNLIINDMDHPLIIKVASIQSARMQVYFIDNEDYF
                     QRKSTLTDENGETFDDNDERAIFFSKGVIETVKKLRWSPDLIHCHGWFTAFAPLMIKK
                     AYADDPLFAHSKVVFSVYNDGFSKPLNENARKKVLMEGISKKDVEILREPTYENLLKL
                     AVNFSDGVILGSPTVNGQVLEHINTAKKPLLPYQDSENYIDIYDEFYNQILNGNSNVS
                     KKK"
     gene            1405..2709
                     /locus_tag="PLW22_02985"
     CDS             1405..2709
                     /locus_tag="PLW22_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOU73700.1"
                     /translation="MFQKRSSIGIRHLGVLIPGLFLSIFSLTSCYNEPGFLGNNLLPD
                     EDIYKVKIDSSFKVSAFTLTKDSLNSFLASEGVMGYVNSEIFGSTKGSFVGRYLPGKS
                     TDGYGGPTATADSIFFYFTASGFYGDSTTELNLRIHEITDTSLFWDKQNALSPINVNT
                     YNPTPFVTATLKGKGSLKLPLPLAFGQSLMDSLALTDSKIFYTKFKGFYVTFDELPGY
                     GGVAYNVSTSNMYLRLYYHYVKQINGKDSTIKTSKTYTFGSRFYEYLHDYTKADPAKK
                     IQHLNDTITQDTVFYTQSLGGVYGKITFPDLTQWRDSMPIVIHRAELIIGKADATTSL
                     PDSTISQLLFYYKDGNEWIGLIKDQITSTNTLSSNGSYRTFRNGYSVNITYHFQRMLK
                     GEYSDNSLYVFPNSNTTIRHGVLKSGQNSTPIKLKITYTKLN"
     gene            2711..4546
                     /gene="glmS"
                     /locus_tag="PLW22_02990"
     CDS             2711..4546
                     /gene="glmS"
                     /locus_tag="PLW22_02990"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOU73701.1"
                     /translation="MCGIVGYIGYRQAFPIVINGLKRLEYRGYDSAGICIVNGDPFLA
                     KQKGKVSDLEAFIGDRPLPGTIGIGHTRWATHGEPNDINAHPHESMNGKFILIHNGII
                     ENYSLLRSKLEKRGYIFKSETDTEVLVNLIEYIYLKGNVTPEIAVRLALTKVVGAYGI
                     VVINRDEPDKLIAARKGSPLVIGVGEGEYFIASDATPIVEFTKSVIFLNDNDVAVLQR
                     NQMVLKTIYNQALEPNIQQIDIDIEAIEKGGYEHFMLKEIFEQPRSIHDTYRGRISSD
                     PFEIHLGGLYSVLPNLTEAKRIILIGCGTSWHAALVGEYLIEDLARIPVEVEYASEFR
                     YRNPIINKDDVVIAISQSGETADTLAAIRLAKEAGALALGICNVVGSSIPRETHAGVY
                     THAGPEIGVASTKAFTAQVTVLIMMAILLGRRRGTLTEERYRELIREVTLVPDKIEKI
                     LQSEELVKVISERFKDSRNFLYLGRGYYFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDDTMPVVVIAIKDSSYEKIVSNVQEVKARKGVVIAVVTEGDTTIKEMADYTIE
                     IPEAGEIVGALLTVIPLQLLAYHIAVMRGCNVDQPRNLAKSVTVE"
     gene            complement(4594..5478)
                     /locus_tag="PLW22_02995"
     CDS             complement(4594..5478)
                     /locus_tag="PLW22_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73702.1"
                     /translation="MIKQLKFPLTFVLIGTLVLSCAVSKKNKQLSDEAKRAFEMNDYQ
                     AALASYEQLIATGKGVTGDVWNKAGIAAWEVGQTEKAIDYLEKAKKENSADAQGLFAL
                     AKAYRKIDNLSREIVNLKLCVDLNQPELLSQARATLFDAYIRSENWNLADSLWNALAP
                     QYQSDVNLKTGYLIVNRKLKNNQKAEMLAKELLKHDKNNTEALEFLGEYHFNRADELY
                     VSEMNAYEKNKTTKQYKQLTEALKKVNADYKISRDYFETLYRLKPDKRYARYLGNIYT
                     RFENKQKADYWYKLAKYK"
     gene            5623..7209
                     /locus_tag="PLW22_03000"
     CDS             5623..7209
                     /locus_tag="PLW22_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73703.1"
                     /translation="MKKLIIAIAITLASPYLYAQETYHHISNIGIYEFIDELNTLGIV
                     NVNTSVKPYSRDFIKTLLTQANEQKEKLNNRQRKELEFYLADFDKDNAAKGKKRFDIL
                     YRSDSLYSITINPLLGVNTIKNDSAFAYNRWVGAEVHGHAGRFGFYASLRDYAQNYRL
                     AMPTYLTREQGGNYKVDGDGGEFSEMRGGITYGWKWGSIGMIKDHVQWGSGYNGTNIL
                     GGRTPSFAMLKLNVKPARWIELNYIHGWLVSQVIDSARTYISFGETRKVYHNKYIAAN
                     LITFKPYRGLNLSVGNSIVYSDLEVQPAYLIPFFFYKSIDHHLNGMSNQVGQNSQMFF
                     DISSYNVKHLHLYATLFLDELSMKNMFNPDKHSNFYSLKAGFRVWDLPLSNLSLTFEY
                     TRTNPLAFKHIIETTTYESNRFNLGHYLTDNADEIHFALSYHPTRGLQLFGSFTKSRK
                     GPDYTKITTERLGLPFMEQVEWEQTFWQVGARYQIINDGYIFIAGGVNNIKNYTSYKY
                     TPNFMLGKQMQVEAGICWGF"
     gene            complement(7206..8723)
                     /locus_tag="PLW22_03005"
     CDS             complement(7206..8723)
                     /locus_tag="PLW22_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU73704.1"
                     /translation="MNRRVSILILLDIFLIGVSFWLTTVIKGVSFQTYLGNYGNGVLI
                     FTLTWILTSLIYNKYSFTSYSIRHLSKNIITSNLIVLAIISILIFLTRNDYYSRFIVF
                     STIAFTTLAELFIYNLWGVLKKTQVLPDDVLGKVERSLRRPRPAPTISEEPIDPTRVK
                     TVQKAILSDFSRDVYCFLEQHTNLFSDKTLIIATTTSFNLLNQPSNTFKTIINLKRVN
                     DIRRINKFFEAANEKIPVGGVFVCMAETQEQRKKRILSKFPPILNYIYYLFDFILKRV
                     FPKFSFTKGIYFLLTRGENRVISRAELLGRLYSCGFDVIDEQEIDKMHYVVSQKIRKP
                     YFDMEPTYGPLIKLRRIGKGGKFIKVYKLRTMHPYSEYLQEYIYRKHNLQEGGKFKDD
                     FRVSTLGRFMRKLWIDELPMIINLLRGELKIVGVRPLSQHYFNLYSKELQERRIKYKP
                     GLIPPFYVDNPKTLEEIMASEIKYLDAYDKHPLFTDLKYFFVAVYNIVFKRYRSN"
     gene            8870..10075
                     /locus_tag="PLW22_03010"
     CDS             8870..10075
                     /locus_tag="PLW22_03010"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOU73705.1"
                     /translation="MLSIREIYKEGYGPSSSHTIGPARAAEIMKQRHPEANYFRVSLY
                     GSLALTGKGHHTDSALRKSFLPKQVDIIWRPEESLPIHPNGMIIEAFSEEDGQLIDFW
                     EVYSIGGGDLKDKTGVLNNNPVYRLTTMTEILAWCKSEGKSLWEFVEDNEGKEIWDFL
                     AKVWHTMRETVKRGIDNEGVLPGPIKLARRASAQYTKALNAHGTMKHIGLLFAFALAV
                     AEENAAGGKIITAPTCGSSGVLPGVLFYLNSEEHITEQKMLRALATAGLIGNLVKHNA
                     SISGAEVGCQGEIGTACAMAAAAMTQIMGGSPTQIEYAAEMAMEHNLGLTCDPVMGYV
                     QIPCIERNAIAATKAYSCAVYALSSDGGHKVSFDKAVETMAETGRDIQVAYRETGIGG
                     LAKNWEAFG"
     gene            complement(10072..11040)
                     /locus_tag="PLW22_03015"
     CDS             complement(10072..11040)
                     /locus_tag="PLW22_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU73706.1"
                     /translation="MAEVVAGIDIGGTNTVIGIVNRNGDILAESTLPTRCNATFDVFV
                     KNLTGAIEQLLMELGHEHRLLGVGVGSPNGSYNLGAIVDAPNLEWKGILPLCKEITLL
                     TGVPSVVTNDANAAALGELLFGAAKGMKNFVVITLGTGLGSGIVVDGKLVVGHDGFAG
                     EFGHVVAKANGRQCGCGKKGCLETYASATGLRRTAFKMIADSNQPSMLRNVTYDKLTA
                     KMITEAAKTGDPLARAAFEYTGLILGTRLADLVAILNPEAIFFFGGLANAGELLMDPV
                     RRYMEDYMFPVFKGKVKLLLSGLQDKNAAVLGAAALIWEELDKSAH"
     gene            complement(11065..11298)
                     /locus_tag="PLW22_03020"
     CDS             complement(11065..11298)
                     /locus_tag="PLW22_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73707.1"
                     /translation="MIAFLKFIFYFFLTLFLLGLIGRIILRLWLRRLYKKINQETGQQ
                     SSDNSKGRNFSFTRKKKIIDKNQGEYVDYEELD"
     gene            complement(11332..14019)
                     /locus_tag="PLW22_03025"
     CDS             complement(11332..14019)
                     /locus_tag="PLW22_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73708.1"
                     /translation="MKRIIATIVFLTLAIAGLVFLFYHWVNKQDYRQFNPIDAIPLNA
                     SLILNIDDFELFTNSVKSNGWWKTLERGDVVKSFADMLNYLDSAAKSDNTVRTAIKGT
                     NTAVAFYNNHPQGSEFLLAHKVPKEINQSDILELIKLQSLGYAKAEEIQIAGNSAIYV
                     EVPEGNYFSLTFTVFGNLLLVSNSQELLVESVKKLKEDAAVESDEQFQSLYQTAATGV
                     AANVFVNINELSKTLSLENNSLALKGVASWIGLDVQVSPTMVIANGLIVSSATNGDYY
                     KIFTRQSPVPFSLHGYMPASTSFFTWYGLPNIPNFMADYSDYIDNLGLTEPYNRNLSG
                     FLAHTGVSIDEFLVKNLDNEVAVLSAKLQNDSTDWFILANTKSGSTTLQQLESFAGTK
                     TIVKHEFKPDQQKSFPIVENPIKWFIPTLLGNSFLKVNDEFVTVIDNVLVFGSSISSL
                     EFIINEYLRNNTLTRTEGYRSIQQRISTSSNLILYSKGMDSYPLSELHLHGKPTQLYK
                     GKTLSSHNFIWQVVGGSQKLFAMLAIKSSQADLTETPNSLKNVNWVCKLSTEPVGKPH
                     LLVNHLNGQPEVLVQDSENSIYLISNDGHILWKRKIGAPILGSVSQVDIYRNKKLQMA
                     FAAGNRIYLVDRNGNDVEGFPVTLPARATSPLSAFDYDSNRNYRFIVACSDKKIYCYD
                     TNGKPVSGWLSFKTETVVSSRIGYVSNQGKDYILIFDQNRPYLLNRRGEERLKPQSFF
                     AKAKNSEFYLVGNSGKGSYLLTTDTLGIIKKLFFDGRVESQVLEPYSPNHTFIMAGKN
                     YFILDRNRVSIYSNEGKLKNAILSSIGDFDTGNLQVMPNNNLVLTNRDESIFGYDFSG
                     KPLSFFPISGNIPIVEVNSRNKQFVTLSKQNGVICFAIK"
     gene            14281..17724
                     /gene="ileS"
                     /locus_tag="PLW22_03030"
     CDS             14281..17724
                     /gene="ileS"
                     /locus_tag="PLW22_03030"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOU73709.1"
                     /translation="MGNLKFKEYKGLDLPQVNKDVLKTWEQQNTFKKSIETRKGHPKF
                     IFYEGPPSANGIPGIHHVMARTIKDIFCRYKTQKGFLVERKAGWDTHGLPVELGVEKM
                     LGITKEDIGTKISVEEYNNTCRKEVMKYTDLWEDLTQKMGYWVDMDDPYITYDNRYIE
                     SLWYLLQKLFEKGLLYKGKTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTVVAQFNVI
                     RNESSQFLFDLTDTPDVYILAWTTTPWTLPSNTALAVNASIKYSIVRTFNPYSGEPLT
                     VVLAKDRQDYYFPAANAELSFNDYQPGDKKVPFKVLSEVEGSKLVGIRYHQLFPWVKP
                     EGDAFRVIAGDFVTTEDGTGVVHIAPTFGADDDRVAKASGIAPLILRDKYRNPEPMVD
                     RRGRFYPIEDLDPDFVKSNVNVDLYREFAGRFVKNEYDSSLPEDAPTLDVDLAVALKK
                     ENKAFRIEKHVHNYPHCWRTDKPVLYYPLDSWFIRVTALKDRMIELNKTINWKPASTG
                     TGRFGKWLENLLDWNLSRSRFWGTPLPIWVSEDRSEMICIGSVAQLKDEIEKAIKAGF
                     MDKNPLANYKEGDFSKENYNSFDLHRPFVDNIVLVSPSGKRMFREPDLIDVWFDSGAM
                     PYAQIHYPFENKDNLNELFPADFIAEGVDQTRGWFYTLHALSTLLFDSVAYKNIVSNG
                     LVLDKNGNKMSKRLGNAVDPWEVIEKYGSDPLRWYMITNAQPWDNLKFDIDGVDEVKR
                     KFFGTLYNTYSFFALYANVDGFSYAEDEIPVSQRPEIDRWILSLLNSLVKEVEEAYED
                     YEPTRAGRAIQDFVIEQLSNWYVRLNRKRFWGGEYDTDKIAAFQTLYTCLETVAMLAA
                     PIAPFYMDQLFTDLNRVTHRHSAESVHLANFPKCNDELIDKNLEEKMEMAQSICSLVL
                     GLRRKVNIKVRQPLQKIMIPILDERFREQLAAVENLILSEVNVKELEYLSDSSGILVK
                     KIKPNFKTLGPRYGKAMKAISEAIAKMTQEDIAAFERNGKFTIKAAEGDINLTLEDVE
                     ILSEDIPGWLVANEGRYTVALDITITEELRMEGIARELINRVQNMRKDSGFEVTDKIR
                     IKIQKHDKLNRAIEVHKSYISTQTLAREIILVDEELNGNSNVIELDDELSTAISVEKI
                     E"
     gene            17780..18157
                     /locus_tag="PLW22_03035"
     CDS             17780..18157
                     /locus_tag="PLW22_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOU73710.1"
                     /translation="MGEKTRYSDEELAEFKEIILQKLEKARRDYEILKSAITMDESND
                     TEDTSPTFKVLEEGASTLSKEEAGRLAQRQLKFIQHLEAALVRIENKTYGICRETGKL
                     ISKERLRAVPHATLSIEAKNSQR"
     gene            18247..18906
                     /locus_tag="PLW22_03040"
     CDS             18247..18906
                     /locus_tag="PLW22_03040"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOU73711.1"
                     /translation="MKFNKKHLAFLIILLILVIDQAFKVLIKTNFAIGDEMRVIGNWF
                     ILHFTENPGMAFGLKFWGIWGKIGLSIFRIAAIVAIAWYLNHLIKQQVKTGIIVGLSL
                     ILAGAIGNIIDSAFYGYLFDSGTTYSSEFNQWVSYYGISKLNFGGYAGFLQGCVVDMF
                     YFPIIDTVLPSWVPIIGGEHFVFFRPVFNIADSSITIGVIYLLLFHRHTLFGHQPNQK
                     P"
     gene            18913..19383
                     /gene="purE"
                     /locus_tag="PLW22_03045"
     CDS             18913..19383
                     /gene="purE"
                     /locus_tag="PLW22_03045"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOU73712.1"
                     /translation="MNRKVVIIMGSKADLDWANQIVNVLKGFGIETVTRIASAHKVPL
                     KCYELIKHYEKENVVFITIAGMSNALSGFTDAQTHCPVIACPPYSDKFGGADVYSSLR
                     MPSGVAPLTILSTENAALAAAKIIGLSNPDIQKKIAEYQEKKRIEIEEADKNIQ"
     gene            19393..20340
                     /locus_tag="PLW22_03050"
     CDS             19393..20340
                     /locus_tag="PLW22_03050"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOU73713.1"
                     /translation="MGIVGVDIDHAASIIANGGVVAFPTETVYGLGANALNPNAVAKV
                     FALKERPSFDPLIVHIASAESINLLSSSNDPRVFQLAERFWPGPLTIVVPKSTVVPGI
                     VTSDLDTVGIRMPNHPIALELIKKSGCVIAAPSANKFGRVSPTRAEHVLKQLPEVDYI
                     LDGGRTTVGIESTVITLDEEGFIILRHGAITHSHLAQVLKPSGAHKPLKGLASPGLLK
                     SHYSPNKPLYIAGETSKPIDPSRAGYIAFGKKPEGNYKRVEYLSETGDMVEAAAHLFE
                     KIHAFEDSDVEYIVADPVPEVGIGIAIMDRLRKAAYRYK"
     gene            complement(20446..21036)
                     /locus_tag="PLW22_03055"
     CDS             complement(20446..21036)
                     /locus_tag="PLW22_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOU73714.1"
                     /translation="MYHIPRTRFWNWDDNPVSQIFWGRVNVEHACSFFYFRKGSRYRK
                     LLHMLKYQGREDIGVFLGRQFGLELKSSELYKPITAIIPVPLHPKRFKERGYNQAEAI
                     ARGLSEAMGVPVVTDVLLRNIYTQTQTKKTRMERMQNIAGAFTICNPEKIEGGHVLLV
                     DDVITTGATLETCTQTLIDSVNVKVSVGTLAYATNT"
     gene            complement(21151..21588)
                     /locus_tag="PLW22_03060"
     CDS             complement(21151..21588)
                     /locus_tag="PLW22_03060"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOU73715.1"
                     /translation="MKKLFLLSLAIMLIVPAMAQHQDKVLGTWLTQYGDSKVTIKKDA
                     KGKFYGEITWLKEPNKDGKPKVDDKNPEPKLQNRPIMGLRMLDGFVYKDNQWVDGTIY
                     NPKEGKTYKCYMWFDDGNYNLLHVKGYIGFSFIGKEVEWTRSN"
     gene            complement(21637..22389)
                     /locus_tag="PLW22_03065"
     CDS             complement(21637..22389)
                     /locus_tag="PLW22_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOU73716.1"
                     /translation="MRSLFTAIIALLPICINAQVGYVQEGNASYYGDNFEGRVTASGE
                     RYSHLKATCAHVSIPFGSLVKITNLDNNQTAIARVNDRGPFVPDRIIDVSKSVAERLG
                     MIGTGIARVRIEVVDNQGIKLNSQEEVSKTAVTDGDKKTTNLQTTEIKTNQQAAPSEA
                     IEYFSVEVQKQTKKGFYVQLGSFREQTNIFLLLADAQEQFGKKTCVQVVAVNNDRLFK
                     LLIGPFTSRSDAEKVKQSAQTKYPGCFIVEAK"
     gene            complement(22391..23053)
                     /locus_tag="PLW22_03070"
     CDS             complement(22391..23053)
                     /locus_tag="PLW22_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOU73717.1"
                     /translation="MPGLQKETVNFLVNLSNNNNREWFQVNRNYYKLAQTDFQRLVFT
                     LIAGISHFDKSIGALEPRDCIFRIYRDVRFSTNKNPYKENFGAFIAPGGRKSSGCGYY
                     IHIQPGGSFASGGLYMAPAPVMKAVRTAMVENRSEFLSIVNNPKFKSTFTWEGVEQAK
                     RTPAGFEFNPELDLFLRLKHITPSRDFTDDELFHPDFDLRAIEVFETLSPLVKFLNQA
                     IP"
     gene            complement(23135..23207)
                     /locus_tag="PLW22_03075"
     tRNA            complement(23135..23207)
                     /locus_tag="PLW22_03075"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23172..23174),aa:Lys,seq:ctt)
     gene            23323..24381
                     /gene="rfbB"
                     /locus_tag="PLW22_03080"
     CDS             23323..24381
                     /gene="rfbB"
                     /locus_tag="PLW22_03080"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOU73718.1"
                     /translation="MERRTILITGGAGFIGSHVVRRFVNSYPNYLIINFDKLTYAGNL
                     QNLTDIEGKSNYKFIKGDIASEAHVDKVFSEYAIDGVIHLAAESHVDRSITDPMAFIR
                     TNILGTTILLNAARKFWNGNWEGKLFYHISTDEVYGSLGAEGFFTEETPYDPRSPYSA
                     SKASSDHLVRAYNHTYGLPAVISNCSNNYGPNQFPEKLIPLSIHNIKNSKPIPIYGKG
                     ENVRDWLYVEDHAAAIDLIFHKAKPGTTYNIGGNNEWKNIDLIRLLCQIMDRKLGRAQ
                     GESEKLITFVKDRAGHDLRYAIDSSKIQRELGWKPSLTFDVGLEKTVEWYLANEQWLN
                     NVVSGDYLKYYEQQYGGR"
     gene            24709..25155
                     /locus_tag="PLW22_03085"
     CDS             24709..25155
                     /locus_tag="PLW22_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOU73719.1"
                     /translation="MAEKPDSRIVEFINEHHVLTLATSFNEEPWCANCFYVYMDDENS
                     LVFTSDFDTKHIQQASHNIYVAGTIVLETSIIGKIQGVQFQGIISQPQGELHERAKKA
                     YLKRFPIAMLMETHLWVVDLTYLKMTDNRLGFGKKLIWSKDLSFQK"
     gene            25170..26159
                     /locus_tag="PLW22_03090"
     CDS             25170..26159
                     /locus_tag="PLW22_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU73720.1"
                     /translation="MILVTGATGLVGAHLLYELCSGGHKVRATRRANSKTHFVEWVFS
                     LYTANSSDLLNLIEWVDAEVLDYQSLVEATKGVSTVFHTAATVSFSPSQEQNILATNV
                     RGTANVVDACLENGVKTLCHVSSIAALGERNDLGVIDESCKWQKSKGQSAYARSKFLG
                     ENEAWRGFEQGLRVVIVNPSVILGPGRWNSGSGQLFSIVSKGMPFYTDGVTGYVDVRD
                     VAKAMVFLTLNTEVSGERFVLNSENLGFRELFTLIANVSNNRKPFIKIPLALIHVLYP
                     LIFILTAGRISKSNLKSAFTKSYYSSDKIKALGFTFTPITQTVQLMAEAKKKS"
     gene            26573..27052
                     /locus_tag="PLW22_03095"
     CDS             26573..27052
                     /locus_tag="PLW22_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOU73721.1"
                     /translation="MDKREILSKKILSGVEYDRMVAFWNFKSRSVVALYGTFDVFHPS
                     VFDLITFASELGQELAVGVKPDADVKAEKGEKFPIYNQDQRAAMVSAHQLVSAVHICN
                     EGVEDFLRRTKPAVAVCCAHASETDKKALEVVKEWGGRVEIFTGSTTSIDDIISKYK"
     gene            27053..28432
                     /gene="radA"
                     /locus_tag="PLW22_03100"
     CDS             27053..28432
                     /gene="radA"
                     /locus_tag="PLW22_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOU73722.1"
                     /translation="MAKTKSVYVCQNCGAESVKWLGRCPSCNEWNTFVEERITKESKS
                     RPGLVEVSRNAIPKPLKEIETSNEKRIDTRIGELNRILGGGIVPGSIVLLGGEPGIGK
                     STLALQLALGMDHQKVLYVSGEESARQVKIRADRINPTNQDCLILNETLLENILTQIE
                     NVQPDLIVIDSIQTLYTDTIESSPGSVSQIRETAATLLRYAKTSGVPVILIGHITKDG
                     SIAGPKVLEHIVDVVLQFEGDQNYLYRILRSSKNRFGSTSEIGIFEMQNSGLVEVTNP
                     SEILLSHRDEQLSGIAVAATIDGARPFLIETQALVSTAVYGTPQRSTTGFDQRRLNML
                     LAVLERRAGFRLANKDVFLNIAGGLKVSDPAIDLAVIAAILSSTLDVAIPGTTCFAAE
                     VGLSGEIRPVNRLDQRVAEANKLGMTRMFVSRYNTKGLDNGNKNCKIIPIGRIEELIR
                     LLFGRESVD"
     gene            28444..29712
                     /locus_tag="PLW22_03105"
     CDS             28444..29712
                     /locus_tag="PLW22_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73723.1"
                     /translation="MRIAVIGFGAAAIGLLEKLKNSNHEVHVFEKSNDIYSSSISGIR
                     ADGKLFVSTEMGGEIYIDPLLQHRFVEYYINFTDKREVETGNSFASEDYYRKFYEKGF
                     LPISSQFFHIGTDQLKEVLFNIYEQFCKSANIHFHFSTEVTEVKEQPDGVIVNGTERF
                     DVAVIAVGRSGHKLINRTISEYPQLIKNNTKVDLGIRYELPNHIVDEINREMYEFKVK
                     YKSRTGYMVRTFCNNPSGFVVTENYGDFTTVNGHAKMREKSGNTNFAILASLVLTEPF
                     NDPIGYGSYIAKLSNLLAGEGKVILQTYSHFKESKRTKNLYRVHPTLEPEKYILGDIN
                     LAFPRRIIESIIDFIENLNTVIPGVANNDNLVYAPEIKFYSNRLNNQISARLKFIGDC
                     SGATRSIIYATAHGYLMAEAIQSNNFNVDI"
     gene            29712..31304
                     /locus_tag="PLW22_03110"
     CDS             29712..31304
                     /locus_tag="PLW22_03110"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU73724.1"
                     /translation="MLKVSDLTVSFSGNPVVSGISFSLMPGEILGFVGESGSGKSVTS
                     LALMGLLPPNAKVEGGSASFSTEKGQVDLLKVDSKTHRQIRGQGIAMIFQEPQTSLNP
                     SMICGKQISEAVELHSNLRGAKAKERCIQLLAEMQLTDPAKVYSSYPHQLSGGQKQRV
                     MIAMALAGNPRILIADEPTTALDVTVQKSILQLLKDIRQRHQIGIIFITHDLSVVAEV
                     ADRIMVMQKGQIVEQGAVTEIFNNPTHPYTRRLIEFRKRINQPVTTTASVTNEPEPIF
                     RVNSINVEYGSNSWFKKGFKAISDVSFTLFKGETLGLVGESGSGKSTIGRTLVKLIET
                     QSGTIEYNGEPLSSLKGSNLLNFRREVQLIFQDPYSSLNPRLTIGQAIMEPFIYHGLG
                     GKREARKAVEELLQMVSLPADSINRYPHEFSGGQRQRIVIARALVLKPKILVCDEILS
                     ALDVSTQAQMLDLLQELKKGMGLTYLFISHDLSVVRSICSRVIVLNRGSIEEQGPAEE
                     IFSRPQSVYTQLLIDAIPGKSL"
     gene            complement(31301..>33043)
                     /locus_tag="PLW22_03115"
     CDS             complement(31301..>33043)
                     /locus_tag="PLW22_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOU73725.1"
                     /translation="AKTFFNTTPDSLTIDQAALLVGVVNAPTRYSPVRNPENAKQRRN
                     VVLGQMLKYGYINQEQFDSISKLPIVLKYQAQDHNQGTATYFREMLRLFMTASKPNPK
                     RYWSQQLYREDSLLWETNPLYGWCNKNTKPDGTPYNVYTDGLKIYTTVNSRMQRYAEE
                     ALAEHLKNDLQPALDKEIKARGGRIFYDITPEQADHIIYLAMRQTQRYRGLVASGASR
                     DSIMANFNEKIPMRVFSWKGERDTLMSPLDSIRYYKRFLRSSFMAMDPHNGHVKAYVG
                     GPDFKHFKYDMVRQGKRQVGSTIKPFLYTLAMQEGYSPCTKVPNVTQTFVVGDTIWAP
                     KNSGSTKYDGQMVTLKWGLSNSVNNISAWIMKQFAPSAVVEMIHQLGIKSYIEPVPSI
                     ILGTFEFSLYEMVGAYGTYVNKGVQVEPIFVTRIEDKNGNVLATFNTKKNEAISEQTA
                     YLMINLLESVVNQGTGVRLRLKYQLPGKIGGKTGTTQHHSDGWFMGVTPNLVAGVWTG
                     AEDRSVHFQNLALGQGANMALPIFGLFMQKVYADPSLGISPNDDWQRPILPGNISINC
                     ENETNYEINEIEIY"
BASE COUNT         9439 a         7148 c         7365 g         9091 t
ORIGIN      
        1 ctcggtgggc atgaaaacta cattaacccc tgcagcctcc agcatggcaa tgtccttttc
       61 gggcattcgg ggatagttct ttaggtcgtt tttatcgttg aattgtgtgg gatttacaaa
      121 aatgctcaca accgtgatat cgttctcagc aacactgcgc tcaaccagcg agacatgacc
      181 tttatgcaat gctcccatgg ttggaacaaa gccaatgctt ttgccctcgc tcctgaagcc
      241 ctgaagcatc gtttttagat ccgatatctt atcaacaacc acacacatag gtaattatag
      301 tttactgagg tatcgacttt gaacttgcaa aggttgtaaa tttttgaata acatgtatgt
      361 tgatttttac tgttgatgcc ctaaaacata tcaaaaagct atataattaa ctttaaatgg
      421 acaccgataa caacttaaat ttgacatgca tactggtatt caattacata acaaattaat
      481 caatatcaag aaaaaagttg taactttgta gcctattata aaaatgcgcc ttggtgattg
      541 atgttttagc tatttaatca aattcggcaa aaggataatc caaaatgaaa gaaaaaaagg
      601 cgaagatact ctttatctct caggagatac ttccatacct gcctgagagt gaggtttcaa
      661 aaatcagtag gtttttacct caagggatac aggaaaaggg tcgagagata agaacattta
      721 tgccaaggta tggatgtgtg aatgaacgcc gaaaccagct acacgaggtg atacgccttt
      781 cggggatgaa cctaatcatc aacgacatgg atcaccccct tatcataaaa gtggcatcca
      841 ttcaaagtgc acgtatgcag gtgtacttca ttgataacga ggactacttc caaaggaaat
      901 ccaccttaac cgatgaaaac ggcgaaacct tcgacgataa cgacgaaagg gctatctttt
      961 tcagcaaggg agtaattgaa accgttaaaa agctacgctg gtcgcccgat ttaatccact
     1021 gccatggctg gttcaccgct tttgccccac tgatgatcaa aaaggcctac gccgacgacc
     1081 ctctgtttgc ccactctaag gttgttttct ctgtgtataa cgatggtttt agcaaaccct
     1141 tgaacgagaa tgctaggaaa aaagtgttaa tggagggtat atctaagaaa gatgttgaga
     1201 ttttacgaga accaacctac gaaaaccttc tgaagcttgc cgtaaacttt tccgatgggg
     1261 ttattttggg tagccctact gttaacggtc aggtgcttga acacataaat acagctaaaa
     1321 aacctttact cccctaccag gatagtgaaa attatattga tatttacgac gaattttata
     1381 accagatact aaatggcaat tcaaatgttt caaaaaagaa gtagtattgg aatcaggcat
     1441 ttgggtgttt taattccagg cctttttctt tcgatatttt cgcttacgtc atgctataat
     1501 gaacccggtt ttttaggcaa caatctttta cccgacgagg atatttacaa ggtaaagata
     1561 gattcctcgt tcaaggttag cgcttttacc cttacaaagg attcgctaaa ctcattcctt
     1621 gcctccgagg gagtaatggg gtatgttaac agtgagattt ttggatcgac caagggaagc
     1681 tttgttggtc gctacctccc cggaaaatca accgatggtt atggcgggcc cactgcaact
     1741 gccgattcaa tatttttcta ctttacagcc agcggttttt acggcgattc caccactgaa
     1801 cttaacctga ggatacacga gattaccgac acctcactat tctgggacaa gcagaacgcc
     1861 ctcagcccta ttaatgttaa tacatacaac ccaacccctt ttgttaccgc aaccctcaaa
     1921 gggaaaggaa gtctgaaact accactccca cttgcatttg gtcaatcgtt aatggactcg
     1981 ctggcgttaa ccgattcaaa aatattttac accaagttta aagggtttta tgtaaccttc
     2041 gacgagttgc ctggttatgg cggtgtagcc tataacgtat caacctcaaa catgtacctt
     2101 aggctatact accactacgt taaacagatt aacggtaagg actcaaccat taaaaccagc
     2161 aagacctata cttttggttc aaggttttac gagtacctgc atgattacac aaaggctgat
     2221 cctgccaaaa aaatacaaca ccttaacgat accattactc aggatacagt tttctacacc
     2281 cagagccttg gcggggttta tgggaaaata actttcccgg acctgacaca atggcgcgac
     2341 tccatgccca ttgtaataca tcgtgccgag ctcattattg gtaaggccga tgccaccaca
     2401 tcgctaccag actcaaccat tagtcagctc ctcttttact acaaggatgg taacgagtgg
     2461 attggactga tcaaggatca gataactagc acaaataccc ttagctcaaa cggctcgtac
     2521 cggacattca ggaatggtta cagtgtcaat ataacctacc atttccaaag aatgctaaag
     2581 ggtgagtata gcgacaatag cctgtatgta ttcccaaact cgaatactac cattaggcat
     2641 ggggtgctta aaagcggaca gaacagtacc ccaattaaac taaaaattac atacaccaag
     2701 ctcaattagt atgtgcggaa ttgttggata tattgggtat aggcaagctt ttcctattgt
     2761 gataaacggt ttaaaacgcc tggagtaccg gggatacgat tcggccggta tttgtattgt
     2821 taatggcgat ccgtttcttg caaaacagaa gggaaaggta agcgatctgg aagcctttat
     2881 tggcgataga ccactccccg gcacaattgg aataggccat acccgctggg ctacccacgg
     2941 cgagcctaac gacataaatg cccaccccca tgagtcgatg aacggcaagt tcatccttat
     3001 ccataatggg attattgaaa actacagcct actccgtagc aagctggaga agaggggtta
     3061 catctttaag tcggaaaccg atactgaggt tctggttaac ctaattgagt acatctacct
     3121 gaaaggaaat gttacccccg aaattgcagt ccgcttagcg ctgacaaagg ttgtaggagc
     3181 gtacggtata gttgtaatta accgcgatga acccgataag ctgattgcag cccgcaaggg
     3241 cagcccgctg gtaatagggg ttggcgaagg tgagtacttt attgcttccg atgccactcc
     3301 aattgttgag tttaccaaaa gtgtaatatt ccttaacgat aacgatgttg ctgtactcca
     3361 gcgcaaccaa atggttctga aaaccattta caaccaagcg cttgaaccca acatccagca
     3421 gatcgatatt gatattgagg caatagagaa aggcggttac gagcacttca tgctcaaaga
     3481 gatattcgag caaccgcgta gcatacacga cacctatcgt ggccgtatct cgagcgaccc
     3541 atttgaaatt caccttggcg gcctgtatag cgtactacca aaccttacag aagccaaacg
     3601 tataattcta ataggctgtg gtacatcgtg gcacgctgcg cttgtgggtg agtacctgat
     3661 tgaggatttg gcccgcatac ccgtagaggt tgagtacgcc tcggagttcc gctaccgcaa
     3721 ccccattatc aataaggatg atgtggtgat agccattagc cagagcggcg aaaccgccga
     3781 taccctggca gcaataagac ttgccaagga agccggagcg cttgcgctag gaatttgtaa
     3841 tgtggttgga tcaagtatcc cccgcgaaac acacgccggc gtttataccc atgcaggccc
     3901 tgaaattggg gttgcatcta ccaaagcatt taccgctcag gtaaccgttc taatcatgat
     3961 ggccatactg cttggccgca ggcgtggtac acttaccgag gagcgttacc gcgaacttat
     4021 tcgcgaggtt acccttgtac cggataaaat tgagaaaata ctacaaagcg aggagctagt
     4081 caaagttatt tcagagcgtt ttaaggattc acgtaacttc ctttaccttg gtaggggcta
     4141 ctatttcccg gttgcgcttg agggcgcact taaactcaag gagatatcgt atatccacgc
     4201 tgaaggttat cccgctgccg agatgaaaca tgggcccatt gccctgattg atgataccat
     4261 gccggtagtg gttattgcaa ttaaggacag cagctacgag aaaattgtaa gcaacgttca
     4321 ggaggtgaaa gcacgcaagg gtgttgtaat tgccgttgta accgaaggcg ataccaccat
     4381 taaggaaatg gccgactata ccattgagat acccgaagca ggcgagattg ttggtgcttt
     4441 gcttaccgtt atcccattac agctactggc ttaccacata gccgttatgc gtggatgcaa
     4501 cgttgatcag ccacgaaact tagcaaaatc ggttacagtg gagtaaatca agcatattct
     4561 ataaataaaa aaagcgcgtt taacgcgctt tttttattta tacttagcaa gtttatacca
     4621 gtaatcggcc ttttgcttat tctcaaaacg ggtatagatg ttacccaggt aacgggcata
     4681 ccttttatcg ggcttcagcc tgtaaagggt ttcaaagtaa tcgcgcgaga ttttataatc
     4741 ggcattcacc ttctttaaag cctcggtaag ctgcttatac tgtttggtgg tcttgttctt
     4801 ctcgtaagcg ttcatttcgc tcacgtaaag ctcatcggcc cggttaaagt gatactctcc
     4861 aaggaattcc agagcttcgg tattattttt atcgtgcttt agtaactcct ttgcaagcat
     4921 ttccgctttc tggttgtttt tcagctttcg gttaacaatc aggtagcctg ttttaaggtt
     4981 tacatcggac tgatactgtg gggctaatgc attccataat gaatcggcca agttccagtt
     5041 ctcgctcctt atgtatgcat caaaaagcgt agcccttgcc tgagatagca attccggttg
     5101 gttaaggtca acgcaaagtt taaggttaac aatctcacgc gaaagattat caattttacg
     5161 gtaggccttt gcaagtgcaa ataagccctg agcatcggca ctgttttcct tcttggcttt
     5221 ttccaggtaa tcaattgcct tttcggtttg tccaacctcc catgcggcaa taccggcttt
     5281 gttccaaacg tcaccggtaa cacctttacc tgtcgcaatt agctgttcat acgaagccaa
     5341 tgcagcctga tagtcgttca tttcaaaagc tcgcttggcc tcatcggata gctgcttatt
     5401 ttttttcgaa accgcacaag aaagtactag cgttcctatt aggacaaatg ttagcgggaa
     5461 ttttagttgt tttatcatta taggctattt taatcgctgc aaatgtaaca ataaggtaac
     5521 aaatttgtta cccaaaacaa atcaagtagc taagttcaca cttaaaccca aatgaaaata
     5581 ttgggttaat gttgtaactt tactaaaaaa attgacgcca caatgaagaa actaataatt
     5641 gcaattgcaa taacacttgc ttcgccctat ttgtatgcac aggaaacata tcaccatatc
     5701 tcaaatatcg gtatctacga gtttattgat gagctaaata cgcttggcat tgtaaatgta
     5761 aataccagcg ttaagcctta cagtcgtgat tttatcaaaa cgttactaac acaggcaaac
     5821 gaacagaaag aaaagctgaa taaccgtcaa aggaaggagc ttgaatttta cttggccgat
     5881 tttgataagg ataatgctgc taaggggaaa aaacgatttg atatcctgta tcgatccgac
     5941 tcactttaca gtattacaat aaatcccttg ctgggtgtaa acaccattaa aaatgatagt
     6001 gcatttgcct ataaccgttg ggttggagca gaggtgcatg ggcatgccgg aagatttggt
     6061 ttttatgcaa gtctccgcga ttatgcccag aattataggc ttgcgatgcc aacctatttg
     6121 acccgagagc aaggcggtaa ctacaaggta gatggagatg ggggtgaatt tagcgaaatg
     6181 agaggcggta taacctatgg atggaaatgg ggcagcatag gaatgattaa ggatcatgtt
     6241 cagtggggct ccggttataa cggcacaaat attctggggg ggcgcacacc atcgtttgcc
     6301 atgctaaaac ttaatgtaaa acctgctagg tggatagagc tgaactacat tcacggttgg
     6361 cttgtatcgc aggtaattga ttccgcaaga acatacataa gctttggaga aacccgaaag
     6421 gtttaccaca acaagtacat cgccgctaac cttataacct ttaaacccta cagggggtta
     6481 aacctttccg tggggaactc aatcgtttac tctgaccttg aggttcaacc cgcctacctt
     6541 ataccatttt tcttttacaa atcaattgac catcacctga atggcatgag caaccaggtt
     6601 gggcaaaact cgcagatgtt ttttgatatc tcatcgtaca acgttaagca ccttcacctt
     6661 tacgccactc ttttcctgga tgagctttcg atgaaaaata tgtttaaccc cgataagcac
     6721 agcaattttt acagcctgaa agcgggcttc agggtgtggg acctcccttt gagcaattta
     6781 tcattaacct ttgagtacac ccgtactaat ccgctagcat ttaagcatat tattgaaaca
     6841 accacatacg agagtaacag gtttaaccta ggccattacc ttaccgataa tgccgatgaa
     6901 atacattttg ccctaagtta tcaccctacc cggggattac agctattcgg aagttttacc
     6961 aaaagccgaa aaggtcccga ttatacaaag attacaaccg aacgactagg actgcccttc
     7021 atggagcaag tagagtggga gcaaacgttc tggcaagtgg gtgctcgata ccagataatt
     7081 aacgatgggt atatctttat cgccggaggg gttaacaaca tcaagaacta cactagctat
     7141 aagtacaccc caaactttat gctgggcaag cagatgcaag ttgaagctgg catttgctgg
     7201 ggcttttagt tgctcctata ccgcttgaat acaatattat aaacggcaac aaaaaagtac
     7261 ttcaggtcag taaacagtgg atgcttatcg taagcatcaa ggtatttgat ttccgaagcc
     7321 attatctcct ccagggtttt gggattatca acgtaaaatg gtggaattag gcctggtttg
     7381 tacttgattc tcctttcctg gagttccttg gagtataggt taaagtaatg ctggcttaaa
     7441 ggccttaccc ccactatttt tagttccccc cgtaggaggt taataatcat aggtagctca
     7501 tcaatccata gtttccgcat gaagcgtcca agggtcgaaa ccctaaaatc gtccttaaac
     7561 ttgccaccct cctgtaggtt atgtttgcgg taaatgtatt cctgtaggta ctccgagtat
     7621 gggtgcatgg ttcggagctt gtaaaccttg ataaatttgc cacccttccc aatacggcga
     7681 agctttatga gagggccata ggtaggctcc atgtcaaagt atggcttgcg gattttttgg
     7741 gaaaccacat agtgcatttt gtcaatttcc tgctcatcaa taacatcgaa accgcatgag
     7801 taaagccttc ctaaaagctc ggccctgctt atcaccctat tctcaccacg tgtaagcaaa
     7861 aagtatattc ccttggtaaa tgaaaactta gggaaaactc gttttaggat gaaatcaaag
     7921 agatagtata tgtagttaag tattggtggg aatttactaa gtattcgttt cttacgctgt
     7981 tcctgtgttt cggccatgca aacaaacaca ccaccaacag gaattttctc gttagcggcc
     8041 tcaaagaact tgttaattcg cctaatatcg ttaacccgct taaggtttat aatggtttta
     8101 aaggtattgc tgggctgatt taaaaggttg aagctggttg ttgtggcaat aataagcgtt
     8161 ttatcgctaa acaggttggt atgttgctcc agaaaacagt acacatcgcg gctaaaatcg
     8221 gagagtattg ccttttgaac tgttttaaca cgggtaggat caatgggctc ctcgctgata
     8281 gtgggtgctg gccgaggcct acggagcgag cgctccacct tgcccaatac atcgtcgggc
     8341 aatacctggg ttttctttag aactccccat aagttgtaaa taaacagttc agccagagtg
     8401 gtgaatgcta ttgtactaaa cacaatgaaa cgtgagtagt aatcgttgcg ggtcaaaaag
     8461 attaaaatcg aaataattgc aagcactatg aggttagaag taataatatt cttggataag
     8521 tgccttattg agtaagaggt aaaggagtat ttattgtaga tgagcgaggt taatatccag
     8581 gttaacgtaa aaattaaaac gccattccca tagttaccca aatacgtttg gaacgaaacc
     8641 ccctttatta cagtggttag ccaaaaggaa acgccaatta ggaagatatc cagcaaaatc
     8701 aggattgata ctcgcctatt catttactaa aattgctaat tattgattaa aggtaacaca
     8761 tttttagcaa actactaatt attctaatta aaaaatgggg ttgttaattt actctaaacc
     8821 cttgcaacag atgtaaaaat gatgttgctt tgccaaaaaa ttaaagtgca tgctatccat
     8881 tagggaaata tacaaggagg gatatgggcc atcgagcagt cataccatag ggcctgcccg
     8941 tgctgcagag ataatgaaac agcgccatcc ggaggccaac tactttaggg tatcgcttta
     9001 tggtagtttg gctctaaccg gcaaagggca ccataccgat agtgcactac gtaaaagttt
     9061 tttaccaaag caggtagata ttatttggcg acccgaggaa agccttccga ttcatcctaa
     9121 cggaatgatt attgaggctt tttcggaaga ggacggtcag cttatcgact tttgggaggt
     9181 ttacagcata ggcggtggcg atttaaaaga taagacagga gttctaaata ataaccctgt
     9241 ttaccggctg acaaccatga ccgagatttt agcgtggtgc aaaagcgagg ggaaaagcct
     9301 atgggaattt gttgaggata acgagggtaa agagatctgg gattttttag caaaggtatg
     9361 gcataccatg cgcgaaaccg ttaagcgtgg gattgataat gagggtgttc taccagggcc
     9421 tataaagctg gctcgcaggg cttcagccca gtacaccaaa gcattaaacg cccatggaac
     9481 aatgaaacat atagggttgc tctttgcttt tgcccttgca gttgctgagg agaacgcggc
     9541 aggggggaaa atcattaccg caccaacctg tggttcgtcg ggggtgctac ccggtgtgct
     9601 tttttacctc aacagcgagg agcatattac ggagcagaaa atgcttagag cgttggccac
     9661 tgcagggtta atagggaatc tggtaaagca taatgcatcg atttcggggg ctgaggtggg
     9721 ttgccagggg gaaataggca cagcctgcgc tatggctgct gcagccatga cccaaatcat
     9781 gggcgggtcg cccacccaaa ttgagtatgc agccgagatg gccatggagc ataaccttgg
     9841 actaacctgc gacccggtaa tgggatatgt tcaaatccct tgtattgagc gcaatgccat
     9901 tgctgccact aaagcatact catgcgcagt ttacgcccta tcgtccgatg gggggcataa
     9961 agtatcgttc gataaggcgg ttgaaaccat ggccgaaaca ggccgcgata ttcaggtggc
    10021 ctaccgcgaa accggtattg gtggacttgc taaaaattgg gaggcttttg gctagtgtgc
    10081 cgatttatcg agctcttccc aaataagggc agcagctccg agcacagcag catttttatc
    10141 ctgcaaacct gaaagcaaaa gttttacctt gcccttaaaa accgggaaca tgtaatcctc
    10201 catgtaacgg cgaacaggat ccattagcag ctcaccggca ttggctaaac ctccaaagaa
    10261 gaaaatggcc tcagggttaa gtatagccac taaatcggca aggcgagttc ctaaaataag
    10321 accagtatac tcaaatgctg ccctagcaag cggatcgcct gttttggccg cctcagtgat
    10381 catcttggca gtgagcttat cgtaggtaac gttgcggagc atgctaggct gattgctatc
    10441 ggcaatcatc ttgaaggcgg ttcggcggag gcctgttgct gaggcgtagg tctcaaggca
    10501 tcccttcttt ccgcatccac actgccggcc atttgccttg gcaaccacat gtccaaactc
    10561 gccggcaaaa ccatcgtgac ctacaaccag cttgccatca acaacaattc cgctacctaa
    10621 ccctgtaccc aaagtgataa caacaaagtt tttcattccc tttgctgccc caaatagcaa
    10681 ctcacccagt gcagcagcat tggcatcgtt ggtaaccaca ctaggaactc cagttaataa
    10741 agtaatctcc ttacataaag ggagtatccc tttccactcc aaatttggag catcaactat
    10801 agcgcccaga ttatatgaac cattaggaga accaacacca acgccaagca acctgtgttc
    10861 atgaccaagc tccatgagca gctgctcaat agccccggtt aagtttttta caaaaacatc
    10921 aaaggtagca ttacagcgtg tgggaagggt actctcggca agtatatcgc cattacggtt
    10981 aactattcct attactgtgt ttgtaccacc aatatcgata cctgctacaa cctcagccat
    11041 acatttgatc aaattaaact atagctaatc taactcttcg taatccacgt attcaccctg
    11101 attcttatcg ataatctttt ttttccgggt gaatgaaaaa tttcgcccct tgctgttatc
    11161 ggacgattgt tgacctgttt cctggttaat ttttttatac aaccgcctta accaaagcct
    11221 taggatgatg cgtcctatca aacccagtaa aaaaagggtc aaaaagaagt aaaaaatgaa
    11281 ttttaaaaat gctatcatta ttctgttgaa acggtgcgca aagatagcta tttatttaat
    11341 ggcaaagcat ataaccccat tctgttttga taaagtaaca aattgcttgt tcctagaatt
    11401 tacctcaaca attggaatat tgcccgatat agggaaaaag ctaagaggtt tgccactaaa
    11461 atcgtaacca aaaatacttt catccctgtt tgtcaaaacc aggttattgt ttggcatgac
    11521 ctgtaggttt ccggtatcga aatcgcctat tgacgatagt atcgcatttt ttaatttgcc
    11581 ttcgtttgaa tatatgctta ccctattcct atcgaggata aaataatttt tccctgccat
    11641 gataaaggta tggttgggtg agtatggttc aagcacttga ctttcgaccc tgccatcaaa
    11701 aaatagtttc ttgattatgc ccagcgtatc ggtggttagc aaatatgacc ctttccccga
    11761 atttcctacc aggtaaaatt cactgttttt tgctttggca aaaaatgatt gaggctttaa
    11821 ccgttcctcg ccacgacggt tcagtaaata cggtcggttt tggtcaaaaa tcaatatata
    11881 atccttaccc tgattgctaa cgtatcctat tcggctacta acaaccgttt ctgttttaaa
    11941 gctaagccaa ccgcttacgg gttttccgtt agtgtcgtag cagtaaatct ttttatcgga
    12001 gcaagccaca ataaaccggt agttacggtt actgtcgtaa tcaaatgccg ataatgggga
    12061 ggtagctcta gcaggcaagg tcaccggaaa accttcaaca tcatttccat tgcggtcaac
    12121 caggtatatc ctgttgcctg ctgcaaaggc catctgtaac tttttgtttc gataaatatc
    12181 tacttggctc acactgccaa gaattggagc cccaatcttt cgtttccaga gtatatgccc
    12241 atcattacta ataagataga ttgagttttc agagtcctga accaaaacct ccggctgtcc
    12301 gttaagatgg ttaaccaaca aatgtggttt gccaacaggt tcagtgctga gcttgcaaac
    12361 ccagttcaca tttttaagcg agttgggggt ttcggtcaaa tcggcctggc tgctttttat
    12421 ggcaagcata gcaaaaagct tttggctacc gcccactacc tgccaaataa agttatggct
    12481 ggagagagtt tttcctttgt atagctgggt tggcttacca tgcaagtgta attcgcttaa
    12541 ggggtatgaa tccattccct tgctataaag aatcagattc gatgatgtgg aaatacgttg
    12601 ctgaatgctc ctatagcctt ctgttctggt aagtgtgtta ttacgcaaat actcattaat
    12661 tatgaattca agcgaactaa tgcttgagcc gaaaaccaaa acgttatcga ttaccgtcac
    12721 aaactcatcg tttactttca aaaagctatt acccaatagg gtagggataa accatttaat
    12781 agggttttca acaataggga aacttttttg ttgatcgggc ttaaactcat gtttgacaat
    12841 ggttttggta cctgcaaacg attcaagctg ctgtagggta gttgaaccgc tcttggtgtt
    12901 tgcaagaata aaccaatctg tgctatcgtt ttgtaatttg gccgatagta cagcaacttc
    12961 gttatcgagg tttttcacta aaaactcatc gattgaaacc cctgtatggg ctaagaaacc
    13021 tgaaaggttt ctattgtatg gttcagttag gccaaggtta tcaatataat cgctatagtc
    13081 ggccataaag ttagggatat tgggcaggcc ataccaggta aaaaatgatg ttgaagcagg
    13141 catgtagcca tgcaaagaaa aaggaacagg gctttgacgg gtaaatattt tatagtaatc
    13201 gccattggtg gctgaactca caataagccc attggcaatt accatggtag gcgatacttg
    13261 aacatcgagc ccaatccagc ttgcaacccc cttaagagca agcgaattat tttcaagcga
    13321 taaagttttg gagagctcat tgatgtttac aaagacattt gctgcaacgc cagttgcggc
    13381 tgtttggtaa agcgactgaa actgctcatc ggattcaacg gcagcatctt ccttcagctt
    13441 tttgaccgat tcaacaagca attcctggct attgctaacc agtagaagat tgccgaacac
    13501 ggtaaaagtt aagctgaaat agttcccttc aggaacctca acataaattg cgctgttacc
    13561 ggcaatttgt atctcctcgg ccttagcata ccctaaactt tgaagtttga taagctctag
    13621 aatatcgctt tggttaatct ccttgggaac tttatgggcc agtaaaaact cgctgccctg
    13681 cggatggttg ttgtaaaaag ctactgcagt attagtgcct ttaatggctg ttctaactgt
    13741 attgtccgat tttgctgcgc tatccaaata attaagcatg tctgcaaaac ttttaacgac
    13801 atcgccccgt tcaagggttt tccaccaacc atttgacttt acagagttag taaacaattc
    13861 aaaatcatca atattcaaaa tcaacgaggc atttagaggg atagcatcaa ttgggttgaa
    13921 ttgccggtaa tcctgtttgt ttacccaatg gtaaaacaga aatactaatc cagcaatggc
    13981 aagtgttaga aatactatgg tagctatgat gcgcttcatc ggtgtaactg tttggcgtgc
    14041 taaagttaca actaatttag gaaggataag ctgatatttg gtgtttagtg agtggggaag
    14101 tggttgatag ttggttgttg atagtgaata tgaaacgcca aacactaaac accaaacacg
    14161 aataatcctc ccctgaataa ctgaacttaa acttttgatt gtctgaaaaa aactctaatt
    14221 ttgtgagatt tttttggaag cgattatcta cacagatttt agtatttaac agcgttttat
    14281 atgggtaacc taaaatttaa agagtacaag ggacttgatt tgcctcaagt gaacaaggat
    14341 gttcttaaga cctgggagca gcagaacacc tttaagaaaa gtattgaaac tcgtaaaggg
    14401 caccccaagt ttatttttta cgaagggcca ccatcagcca atggaatacc cggcatccat
    14461 cacgtaatgg cacgcaccat taaggatatt ttttgtcgat ataaaaccca aaaaggtttt
    14521 ttagttgagc gtaaagcagg atgggacacc catggtttgc ccgttgaact tggcgttgaa
    14581 aaaatgcttg gtataaccaa ggaggatatt ggcaccaaaa tatcagttga ggagtacaac
    14641 aatacctgcc gtaaggaggt aatgaaatat accgacctat gggaggacct tacccaaaaa
    14701 atgggctact gggtcgatat ggacgacccc tatatcacgt acgacaaccg ttacattgag
    14761 tcgctttggt atcttttaca aaagcttttt gagaaaggtt tactctacaa ggggaaaacc
    14821 atccaaccat actctccggc tgccggaacc ggtttaagca cccacgagct taaccagccc
    14881 ggatgctacc gcgatgttaa ggataccact gtggtagcac agttcaatgt tatcaggaac
    14941 gagagtagcc agttcctttt tgacctaacc gataccccag atgtatacat actggcttgg
    15001 acaaccacac cctggactct accatcgaac accgcactgg cggtaaatgc cagcatcaag
    15061 tatagcatcg ttagaacctt taacccatac agcggtgagc cattgactgt tgtactggcc
    15121 aaggacaggc aggattatta ctttccggca gcaaatgccg agctgtcgtt caacgattat
    15181 caaccaggcg acaaaaaggt tccctttaag gttttatcgg aggttgaggg ttcaaagctt
    15241 gtaggtatcc gataccatca actattcccc tgggtgaaac ccgagggcga cgccttcagg
    15301 gtgattgccg gcgattttgt tactaccgag gatggtaccg gtgtggttca tattgcacca
    15361 acctttggtg ccgacgacga ccgagtggca aaggcatcgg gtattgctcc gctaatactt
    15421 cgcgacaagt accgcaatcc tgaacccatg gtcgataggc gcggaagatt ttacccaata
    15481 gaggacctcg accctgactt tgtgaaaagc aacgtaaatg ttgaccttta cagggagttt
    15541 gccggtagat ttgttaaaaa cgagtacgat tcaagcctgc ctgaggatgc accaacactt
    15601 gatgttgacc ttgcggttgc cctaaaaaag gaaaataagg cattcaggat tgaaaaacac
    15661 gttcacaact acccgcactg ctggcgcacc gataagcctg tactctacta cccactcgat
    15721 agctggttta tccgtgttac agcccttaag gataggatga ttgaactgaa caaaaccatt
    15781 aactggaaac cggccagtac aggtaccggg cgatttggca aatggctcga aaaccttttg
    15841 gattggaatc tttcccgttc gcgtttctgg ggtacaccac tgcccatctg ggtttcagaa
    15901 gatcgcagcg aaatgatttg tattggttcc gttgcccagc ttaaggatga aatagaaaag
    15961 gctattaagg ctgggttcat ggataagaac ccgttggcca actacaagga gggtgacttt
    16021 agcaaggaaa actataacag cttcgacctg catcgcccat ttgttgataa tattgtgctt
    16081 gtttcgccaa gcgggaagcg tatgttccgt gagcccgacc taattgacgt ttggtttgac
    16141 tctggagcaa tgccatacgc tcaaattcac tacccctttg agaacaagga taaccttaac
    16201 gagttgttcc cagccgattt tattgctgag ggtgttgatc aaacccgtgg ctggttctat
    16261 acactccatg cgctgtcaac tctacttttc gattctgtag cctacaagaa tatcgtttcc
    16321 aacggattgg tgcttgacaa gaacggtaac aagatgagta agcgccttgg caatgccgtt
    16381 gacccgtggg aggtaattga aaagtacggt tctgatccct tacgctggta catgattacc
    16441 aacgcacaac cctgggataa ccttaagttc gatattgatg gtgttgacga ggttaagcgt
    16501 aagttctttg gaactcttta caacacctac tcgttctttg ctctttacgc caatgtggat
    16561 ggattttcat atgctgagga cgaaattccg gttagtcagc ggcccgaaat tgaccgctgg
    16621 atactttcgc tgcttaacag cttggtgaaa gaggttgagg aggcttacga ggactatgaa
    16681 cctacacgtg ctggccgagc aattcaggat tttgtaattg agcagctcag caactggtat
    16741 gtaaggctta atcgtaaacg cttctggggt ggcgagtacg ataccgacaa gattgcagct
    16801 ttccaaaccc tttatacctg tttggaaaca gtagccatgc tggctgcccc cattgcccca
    16861 ttctacatgg atcagctgtt cactgacctt aaccgtgtta cacatcgcca ttcagccgaa
    16921 agcgttcacc ttgcgaactt cccaaaatgc aacgatgaat tgattgacaa gaaccttgag
    16981 gagaagatgg aaatggccca atcaatttgc tcactggtac ttggattgcg acgtaaggtt
    17041 aacatcaagg ttcgtcaacc actacaaaag attatgattc caatccttga tgaaaggttc
    17101 agggagcaac ttgcagcagt tgaaaacctc attcttagcg aggttaacgt taaggaactt
    17161 gagtacctta gcgactcatc ggggattctg gttaaaaaga taaaaccgaa ctttaaaacc
    17221 cttggccctc gctacggaaa ggctatgaaa gcaatttccg aagccattgc caaaatgaca
    17281 caggaagaca ttgctgcttt tgagcgtaac ggaaagttca ccataaaggc agccgaggga
    17341 gatatcaatc taacccttga ggatgttgaa attctctcgg aagatattcc aggatggctt
    17401 gttgcaaacg aaggacgcta tacggttgcc cttgatatta ccattactga ggagcttcgc
    17461 atggagggta ttgcccgtga gcttatcaat agggttcaaa acatgcgtaa ggattcaggc
    17521 tttgaggtaa ccgataaaat ccgcatcaaa atacagaaac acgacaagct aaacagggct
    17581 attgaggttc acaagagcta tatttcaacg caaacccttg cccgtgaaat tattttagtt
    17641 gatgaggaac taaatggaaa ttctaacgta atagagttag acgatgaact ttcaactgct
    17701 atttctgttg aaaagattga atagatgagt tggtttttta ctacaatttt tttatatctt
    17761 tagcaaaatt taaaaagcca tgggagagaa gaccagatat tcggatgagg agttagcaga
    17821 attcaaggaa attattctgc agaaactcga aaaggcacgt agggattacg aaatccttaa
    17881 gagtgccatt accatggatg agagtaacga taccgaggat acctcgccaa cctttaaggt
    17941 tttggaggag ggtgcttcaa ccctatcgaa ggaagaggct ggccgcttgg ctcaacgcca
    18001 gctcaagttt atccaacatc ttgaggctgc cctggtacgt atcgagaaca aaacctatgg
    18061 tatttgccgc gaaaccggca aactgatctc aaaagaacgt ctacgtgctg ttccacacgc
    18121 aacgctcagc attgaagcca aaaatagtca acgatagctg taagtgttaa tatgtattac
    18181 tcaggaaagc gaccatttgg tcgcttttac tttttgtatt agttttacgc cttaaacagt
    18241 tgacctatga aattcaataa gaaacattta gcctttctaa taatccttct tatccttgtt
    18301 attgatcagg cctttaaggt tttgataaaa accaactttg ccataggcga tgagatgagg
    18361 gtaattggta actggttcat tcttcacttt actgaaaacc ccggtatggc cttcgggtta
    18421 aagttttggg gtatttgggg taaaattggc ttaagcatat tccgtattgc agcaattgtt
    18481 gccattgcat ggtatttaaa tcatcttatt aagcaacagg taaaaacggg aattatagta
    18541 ggcctttcgc tcatccttgc cggtgccatt ggaaatatta tcgatagcgc attctacggt
    18601 tatctgttcg atagtggtac cacctatagc tcggagttta accagtgggt atcgtactat
    18661 ggcatatcaa agctaaactt tggaggatat gccggattcc ttcagggatg tgtagttgat
    18721 atgttctact tcccaattat cgatactgtg ctaccctctt gggttcccat tattgggggt
    18781 gagcacttcg tttttttcag gcctgttttt aacattgccg attcatccat aaccatcggg
    18841 gtgatatacc tgctgctgtt tcatcggcat actctgtttg ggcatcaacc taaccaaaag
    18901 ccataatcca tcatgaaccg caaagttgta atcattatgg gctcaaaggc tgaccttgat
    18961 tgggccaacc aaattgttaa cgtgctaaag ggttttggaa ttgaaaccgt aacacgcatt
    19021 gcatcggccc ataaggtgcc gctaaaatgc tatgagctga ttaaacatta cgaaaaggaa
    19081 aacgtagtgt ttataaccat tgctggcatg agcaacgcct taagcggttt caccgatgct
    19141 caaacccact gcccagtaat tgcctgccca ccatacagcg ataagtttgg tggtgccgat
    19201 gtgtattcaa gccttcgtat gccatcgggt gttgctcctc ttactattct tagcactgaa
    19261 aacgcagccc ttgctgccgc aaaaattatt gggctttcca atcccgatat tcagaaaaag
    19321 attgctgaat accaggaaaa gaagcgaatt gagattgaag aggccgataa gaatattcag
    19381 taattgaagt tgatgggaat agttggggtt gatatcgacc acgctgcatc aattattgcc
    19441 aacgggggag ttgtagcttt tcctaccgaa acggtttatg ggttgggggc taatgccctt
    19501 aaccccaatg ccgtagcaaa agtttttgca cttaaggagc gcccctcgtt cgatccctta
    19561 attgttcata ttgcttcagc tgaaagcata aaccttttat catcaagcaa cgaccccaga
    19621 gtatttcagc tagcagaacg gttctggcca ggcccactaa ccatagttgt tcccaaaagt
    19681 accgttgtcc ccggcattgt gacctccgac ctcgatacag ttggcatacg catgccaaac
    19741 catcccattg ccctggagct tattaaaaaa tcgggatgcg taattgcagc ccccagtgcc
    19801 aataagtttg gaagggttag ccccacacgc gcggagcatg tgctaaagca gttacctgag
    19861 gtggactata ttcttgatgg agggagaaca accgttggaa tagaatcgac agtaataacg
    19921 ctcgacgagg aaggttttat tattctgcgt catggtgcaa taacccatag ccatttggca
    19981 caggtgctga aaccttcagg tgcccataag cctttaaaag gcttagcatc gcccggactg
    20041 cttaaatcgc actacagccc aaacaaacca ctatacattg ctggtgaaac cagcaaaccc
    20101 attgacccat cgagggcggg atatattgcc tttgggaaaa aaccagaagg taactacaag
    20161 cgtgtagagt acctatcgga aacgggcgat atggttgaag cggcagcgca cctttttgaa
    20221 aaaatccacg cctttgagga ttccgatgtg gaatacatag ttgccgatcc tgttcctgag
    20281 gtaggcatcg gaattgccat tatggacagg ctccgtaaag cggcttacag gtataagtaa
    20341 agttgttcgt ttatagttga tagttgttgg ttgatggtga acctaaacct ttactcttta
    20401 tcctatatcc ttaaaccttg aacctttatc ctttatcctt aaaacttaag tattagttgc
    20461 ataggcaaga gtgccaacgc taactttaac attaacagaa tcgataaggg tttgggtgca
    20521 ggtctcaagt gtggcccctg tggtaataac atcgtcaacc agcaggacat gccctccttc
    20581 tattttttcg gggttgcaga tagtaaatgc cccggcaata ttctgcatgc gctccatgcg
    20641 cgttttcttg gtttgggttt gggtataaat gtttctgagc aaaacatcgg tcaccaccgg
    20701 aacgcccatg gcttccgata atccccttgc aatggcctcg gcctgattat aaccgcgttc
    20761 cttaaatcgt ttagggtgta gtggcacagg gattattgca gtaattggct tatatagctc
    20821 cgagcttttc agctcaaggc caaactgcct accaagaaac acgccaatat cctctcgccc
    20881 ttggtatttt aacatatgca atagcttacg gtagcggcta cccttgcgga agtaaaagaa
    20941 tgagcaagca tgctccacat tcaccctacc ccaaaagatt tgcgatactg ggttgtcgtc
    21001 ccagttccag aatcgggtac ggggaatatg atacaaacag gaagtgcaaa gcacctcctc
    21061 gtatagcgaa agactatcgt ggcaaatggt gcaaacgtta gggtaaaaaa gatttataaa
    21121 atcgtatgct acacgtttta ccccatgtgt ttagttgctt cgggtccact ccacctcttt
    21181 cccaatgaaa gagaaaccta tataaccctt aacgtggagc aagttatagt taccatcgtc
    21241 aaaccacatg tagcacttgt aggtttttcc ctctttgggg ttatagatag tgccatcaac
    21301 ccactggttg tccttgtaaa caaagccatc gagcattctc aaacccataa ttggcctatt
    21361 ctgtagctta ggttcaggat tcttatcgtc aaccttgggt ttcccatcct tattgggttc
    21421 cttaagccaa gtaatctcgc cataaaattt tcccttggca tccttcttaa tggttacctt
    21481 ggagtcgcca tactgtgtaa gccatgtacc aagaaccttg tcctggtgtt gtgccattgc
    21541 cggcactatt agcataatag ccaatgatag caaaaaaagc tttttcatag taatttaggt
    21601 tggttatgaa tttcaatatt agtaattttt tatcaattat ttagcctcaa caataaaaca
    21661 gcccggatat ttggtttgag cggattgttt taccttttcg gcatcggaac gggatgtaaa
    21721 cggtcctata agtaacttga aaaggcggtc gttgtttacc gcaacaacct gaacgcaagt
    21781 tttctttcca aactgctcct gcgcatcggc tagcagcaaa aagatattgg tttgctcccg
    21841 gaaactgccc agctggacat agaatccttt tttggtttgc ttttgaactt cgaccgagaa
    21901 atactctatc gcttcggaag gtgcagcttg ttggttggtt ttaatctcag tagtttgtaa
    21961 attggtagtt ttcttatccc catcagttac tgccgttttc gatacctcct cttgggaatt
    22021 aagcttaatc ccctggttat ctacaacctc aattcttacc cgtgctatac ctgtaccaat
    22081 catccctaac ctttccgcta ccgatttgga aacatcaatt attctatcgg gtacaaaagg
    22141 gcccctatcg tttacgcgtg ctattgccgt ttggttatta tccaggttgg ttattttaac
    22201 cagcgaacca aacggtattg acacgtgtgc acaggtagct ttcaggtgac tgtagcgttc
    22261 ccccgatgcg gtaacccttc cctcaaaatt atcgccgtaa tacgatgcgt tgccctcctg
    22321 aacatatcct acctgagcat tgatgcatat gggtaatagg gcgattattg ctgtaaatag
    22381 gctgcgcatt ttatggtatg gcttgattta gaaatttaac cagtggacta agcgtttcaa
    22441 acacttcaat tgccctaagg tcgaaatcgg gatgaaagag ttcatcatcg gtaaaatcgc
    22501 gtgaaggggt tatgtgtttc aacctgagaa atagatcgag ttcgggattg aactcaaaac
    22561 ctgcaggtgt gcgcttagcc tgctcaactc cctcccaagt aaaggttgat ttgaacttag
    22621 ggttgttaac tatggaaagg aactcgctcc tattttcaac cattgccgtg cgtaccgctt
    22681 tcataacagg cgcaggggcc atgtacaaac ctcccgaagc aaatgaaccc ccgggctgga
    22741 tatggatgta atacccacaa ccactacttt ttcggccacc cggagctata aaggccccaa
    22801 agttctcctt gtaaggattt ttgttagtgg aaaagcgaac atcgcggtaa atacgaaaga
    22861 tgcaatcgcg tggttcaaga gcacctatcg atttatcgaa atggctaata cctgcaatca
    22921 gtgtgaacac aagccgttga aaatcggttt gggccagctt ataataattg cggttcacct
    22981 gaaaccactc acgattattg ttgtttgaga ggtttacaag aaaattaacg gtttcttttt
    23041 gtagtccggg catgggggag atggtttact aacaaaatta gcaaaatatt ttatgaccca
    23101 aataaaaaag ccactcctgt ggagtggctt tctgtcggct cggtggggat cgaacccacg
    23161 accccatgat taagagtcac gtgctctacc agctgagcta cgagccgtta tttttgtggc
    23221 tgcaaattta gtatcttttt tatccaacac aaaatgtcat ccaaaaattt actttcttta
    23281 caaaaaattt ttgttaaaca cttgtaaatt caggaaagcg gaatggaacg cagaactatt
    23341 ctaattaccg gtggcgcagg attcattggc tcgcatgtgg ttcgccgatt tgtaaacagt
    23401 taccccaact acctgattat caattttgat aagctaacct atgctggtaa cctgcaaaac
    23461 cttaccgaca ttgagggcaa gtccaattac aaatttatta aaggcgatat agccagcgag
    23521 gcccatgttg acaaggtttt tagcgagtat gccattgacg gtgttatcca cctggcagcc
    23581 gagtcgcacg tggacaggtc gataaccgac cccatggcat ttatccgtac aaacatactt
    23641 ggaaccacca ttcttctgaa cgcagcccga aagttttgga atggcaactg ggaaggaaag
    23701 cttttctacc acatttccac cgatgaggtt tacggcagcc ttggggctga agggttcttc
    23761 actgaggaaa ccccttatga cccccgcagt ccatactccg cttcaaaggc atcgtcggat
    23821 catttggtaa gggcctacaa tcacacctat ggcctacccg ctgtaatatc gaattgctcc
    23881 aacaactacg ggccaaacca gtttcctgag aagcttatac cactatctat ccacaacata
    23941 aagaacagta aacccatccc aatttacggt aaaggggaaa atgttcgcga ctggctgtac
    24001 gtggaagatc atgcagccgc tatcgatctg attttccata aggcaaagcc tggcacaacc
    24061 tacaatattg ggggtaacaa cgagtggaag aatattgacc ttatcagact gctatgtcaa
    24121 ataatggaca ggaaactcgg tagagcccag ggggaatcgg aaaaactgat aacctttgta
    24181 aaggacaggg ctggtcatga cttacgctac gccattgact ccagcaaaat tcagcgcgag
    24241 ctgggctgga agccatcgct aacttttgat gtgggcctgg aaaaaaccgt tgaatggtat
    24301 ctggccaatg agcaatggct taacaatgtg gtaagcggcg attacctgaa atactacgag
    24361 cagcagtacg gagggcgcta ggttagtgta tagtaatagg ggcattggag aatgtgagga
    24421 agttagagga gtatagagcg ttaaacgtaa gccgtaagcc gttaaacgaa atcgagcaac
    24481 ccctattgtc attaggagcc tagcgaggaa tctcatttga aagtggaatt gagaagaagt
    24541 tataaagaag cttgaggaag ttagagaaat gtagagagag ccgtttaaat aaacccgaac
    24601 aacaaataac tacacccaga ggggcagggc aaagtgtatt ttcactacca actatcaact
    24661 accaactatc aactaaattt tttacctttg cttgattaaa agtttatcat ggccgaaaaa
    24721 cccgatagca ggattgttga gtttatcaac gagcatcatg ttttaacgct ggctacaagt
    24781 ttcaacgagg aaccctggtg cgctaactgt ttctatgttt acatggacga tgaaaacagt
    24841 cttgtgttta cttccgattt cgacaccaaa cacatccaac aggccagtca taatatctac
    24901 gttgcgggga ctattgtgct tgaaacaagc atcattggca aaatccaggg agtgcagttt
    24961 cagggaataa tatcacaacc acaaggcgaa ctgcatgaga gagccaagaa ggcttacctt
    25021 aagcgttttc caattgccat gctaatggaa acccacctat gggttgttga ccttacctac
    25081 ttaaagatga ccgataaccg gttagggttt ggtaaaaagc taatttggtc aaaggaccta
    25141 tcgttccaga aataaattca cccaacagta tgatacttgt aactggtgct actggactcg
    25201 taggtgctca tttgctttat gagctgtgtt caggggggca caaggttaga gctacccgaa
    25261 gggcaaactc aaaaacgcat tttgtagagt gggttttctc actatacacc gctaattctt
    25321 cggacttact aaatctgatt gaatgggttg atgctgaggt gttagattac caatcgttgg
    25381 ttgaggctac taagggcgta tcaaccgttt ttcatacagc cgctacggtc tcgtttagcc
    25441 catcgcagga gcagaacata cttgccacca atgttcgtgg gactgctaat gttgtggatg
    25501 cctgtcttga gaatggggtt aaaacgttgt gccacgttag ctccattgca gccctaggcg
    25561 aaaggaacga cttgggagta attgatgaga gctgcaagtg gcaaaaaagt aagggacaga
    25621 gtgcctatgc ccgaagcaag tttttaggtg aaaacgaggc ttggcgtggt tttgaacagg
    25681 ggttaagagt ggtaatagtt aatccttcag taattctcgg tcccggacga tggaattcgg
    25741 gtagtggaca gctgttcagc atagtatcga aagggatgcc attttatacc gatggcgtaa
    25801 ccggttatgt tgatgttcgc gatgtggcaa aagccatggt ctttcttact ctaaacaccg
    25861 aggttagcgg agagcgattt gttcttaatt cagaaaacct tggcttcagg gaacttttta
    25921 ctttaatcgc taatgtttcc aacaatagaa aaccttttat aaagatacct ttggcattaa
    25981 tacatgttct ttacccgctg attttcattc ttacagccgg aagaatatca aaaagtaacc
    26041 tcaaatcggc atttacaaaa tcgtactact catccgataa aattaaagca cttgggttta
    26101 cattcacacc cattacccaa acggttcaac taatggctga ggcgaaaaag aaaagttaag
    26161 aaagagaatg ctggggtcat gaattcacca tcaacgaaca gctaaaactt tcatcctgag
    26221 cgctctgtct cgccttggcg gggagcgaag gatttcagta aagggtaaaa gcgataaagg
    26281 agcaaagaga tgcttcggcg agctcagcat gacaggatgt ttcagagtag tctatcccaa
    26341 tatttcagaa cgggtcacag gtcaacctta aaccattaac ttttagcctt tatcctttat
    26401 ccattatccc ttattcttta tcctttaacc tttatccttc cttcccgctt tccgtcaaac
    26461 cctctttttg tctgcaatca aacgctaaac gctaaatacc aaacttgatt ttcacccatt
    26521 atctctgaaa atttgtattt ttacttgctt aaatctttaa acgttttgct ttatggataa
    26581 aagagaaatt ctttcgaaaa aaatacttag tggtgtagag tacgaccgaa tggtagcatt
    26641 ttggaacttt aaatcgcgtt cggtagtagc cctttatggc acctttgatg tttttcaccc
    26701 gtcggttttt gacctaatca ccttcgcctc tgagttaggt caggagttgg cagtaggcgt
    26761 taagcccgat gccgatgtta aggccgaaaa aggcgaaaaa tttccaattt ataatcagga
    26821 tcaacgggct gcaatggttt cggcgcatca gctggtttca gcagtgcata tttgcaatga
    26881 aggggttgaa gatttcctcc gcaggacaaa acctgctgtg gcagtttgct gtgcgcatgc
    26941 atccgaaacc gataagaaag cccttgaggt ggttaaggag tggggaggcc gtgttgagat
    27001 tttcacgggt agcaccacca gtatcgacga tatcatttca aagtataagt aggtggctaa
    27061 aaccaagagc gtttacgttt gccagaattg cggggccgag tcggtaaaat ggctggggcg
    27121 ctgcccctcg tgcaatgagt ggaacacctt tgtagaggag cggataacca aggagtccaa
    27181 aagccgacca ggactggtag aggtttcccg caatgcaata ccaaagccct taaaggagat
    27241 tgaaaccagc aatgagaagc gcatcgatac ccgcataggc gagctaaacc gaatactagg
    27301 agggggaatt gttcctggtt caattgtttt gcttggaggt gaaccgggca taggaaaatc
    27361 aactttagct ttgcagcttg ccctgggtat ggatcaccaa aaggtgcttt acgtttcggg
    27421 cgaggagagt gcccgtcagg tgaaaatccg tgccgatagg attaatccta ccaatcagga
    27481 ttgcctaatc ctgaacgaaa cgcttttgga gaacatactc acccagattg agaatgttca
    27541 gcccgattta attgttattg actccattca aaccctgtac accgatacaa ttgagtcgtc
    27601 gccgggaagc gtttcgcaga ttcgcgaaac ggctgccacg ctattgcgtt acgcaaaaac
    27661 atcgggtgtt cccgtcatac ttattggtca cataaccaag gatggtagca tagctggccc
    27721 caaggtgctg gagcatattg ttgatgtggt attacagttt gagggcgatc agaactacct
    27781 ttaccgaatc ttacgttcat caaagaatcg gtttggctca acatcggaga ttgggatttt
    27841 tgagatgcaa aacagtggct tggttgaggt aaccaatcca tcggagatat tactatcgca
    27901 ccgcgatgag cagcttagcg gcattgcagt tgccgctaca atcgatggtg caagaccatt
    27961 tctaattgag actcaggcgc tggttagcac agcggtttac ggtacacctc agcgatccac
    28021 cacaggcttt gaccagcgaa ggcttaacat gcttttggct gttcttgagc gtcgcgcagg
    28081 ttttaggctt gccaacaagg atgttttcct gaacattgcc ggagggctta aggttagcga
    28141 tccggctatt gaccttgctg ttattgcagc catactatcg tcaacgctcg atgttgcaat
    28201 acctggcaca acatgctttg ctgcggaggt tggcctttct ggcgaaattc gccctgtgaa
    28261 ccgtctcgac caacgtgtgg ctgaggccaa taaactaggg atgacccgca tgtttgtatc
    28321 gcgctacaat accaaagggc tcgataacgg aaataaaaac tgtaagatta ttccaatcgg
    28381 gagaattgag gagttaattc gactcttatt tggacgggaa tccgtggact aaaaataatt
    28441 gatatgagaa ttgcggttat cggttttgga gcagcagcca tcgggctgct cgagaagtta
    28501 aagaatagca accacgaggt gcacgttttt gagaaaagca acgatatcta ctcgtccagc
    28561 atatcgggca tacgtgccga tggaaagcta tttgtatcaa ccgagatggg aggtgaaatt
    28621 tatattgatc ccttgctgca gcataggttt gttgagtact acatcaattt tacggataag
    28681 cgtgaggttg aaaccggcaa ctcattcgca tcggaggatt actaccgtaa attctacgaa
    28741 aaaggatttc tacccatttc atcgcaattc ttccacattg gaaccgatca gctcaaggag
    28801 gttctcttca atatctatga gcaattttgc aaatcggcta atatccactt tcattttagc
    28861 actgaggtaa ctgaggtaaa agagcaacct gatggtgtaa tagttaacgg aaccgaaagg
    28921 ttcgatgtgg cggttatagc ggttggtagg agtgggcata agctgattaa cagaaccatc
    28981 tcggaatatc cacagcttat aaagaataat actaaggttg atttgggtat tcgctacgag
    29041 ttacccaacc acattgtgga tgaaattaac cgtgagatgt atgaattcaa ggtgaaatac
    29101 aaaagccgta ccggttacat ggttcgtacg ttctgcaaca acccatcggg gtttgtggtt
    29161 actgagaact atggcgattt caccacggtt aacggacatg ccaagatgcg cgaaaaaagc
    29221 gggaatacca actttgccat tctggcatcg cttgtgctaa ccgaaccgtt caacgatccc
    29281 attggctatg gctcatacat tgctaagcta tcgaaccttt tggcgggtga ggggaaagta
    29341 attctacaaa cctacagcca ctttaaggaa tccaaacgca ctaaaaacct ttacagggtt
    29401 catccaaccc ttgagcccga aaaatacatt ttaggtgata taaaccttgc attcccacgt
    29461 aggattattg agagtataat tgatttcatt gaaaatctta acacggttat tcccggggtg
    29521 gccaataacg acaatctagt atatgctccc gaaataaagt tctactccaa taggctaaat
    29581 aatcagattt cggcaaggtt gaaatttatt ggcgattgct caggggcaac ccgttccata
    29641 atctatgcaa cagcacatgg ttacctgatg gccgaagcca tacagtccaa caattttaat
    29701 gttgatatct gatgctaaag gtatccgatc tcacagtatc gttttctggc aacccggtgg
    29761 tttcagggat aagttttagc ttgatgcccg gcgaaatact tggatttgtt ggcgaatcgg
    29821 gatcgggaaa atcggtaaca tcgttagccc ttatggggtt gttgccaccc aatgcaaaag
    29881 tggaaggtgg ttcagcatct ttcagcactg aaaaagggca ggtcgatctt ttaaaagttg
    29941 atagtaaaac gcatcggcaa atcaggggac aaggcattgc aatgattttt caggaacccc
    30001 aaaccagcct taacccttcc atgatatgtg gtaagcaaat atcggaggct gttgagctac
    30061 attccaatct taggggcgca aaggctaaag agcgatgcat acagttgctt gcggaaatgc
    30121 aattaaccga tcctgctaag gtttatagca gctatccgca ccagcttagc ggaggtcaaa
    30181 agcaaagggt tatgatagcc atggcacttg cgggtaaccc ccgtatcctt attgccgacg
    30241 agccaaccac cgccctcgac gttaccgttc aaaagagcat tttacagcta ctgaaggata
    30301 ttcgccaaag gcatcaaata ggcattatct tcatcactca tgatttgtca gtagttgctg
    30361 aggttgccga tagaattatg gttatgcaga agggacaaat tgttgagcag ggcgctgtaa
    30421 ccgaaatttt taacaatcct acacatccat acacccgtcg gcttattgag ttccgtaaac
    30481 ggataaatca acctgttact acaacagcaa gtgtcacaaa tgagcctgaa cccattttcc
    30541 gtgtaaattc aatcaatgta gaatacggta gcaatagctg gtttaaaaag ggctttaagg
    30601 caattagcga tgtttcattt actttattta aaggcgaaac tttaggtctg gtgggcgagt
    30661 cgggttcagg taaaagtacc attggtcgta ctttggtaaa attaatcgaa acccaaagcg
    30721 gaaccattga atataatggg gaaccattga gcagcctaaa agggagcaat ctgcttaatt
    30781 ttcgacgcga ggttcagctt atttttcagg acccatactc atcgctaaat ccgaggctaa
    30841 ccataggcca ggccataatg gagcctttta tctatcatgg tttgggaggt aaaagggagg
    30901 cccggaaggc ggttgaggaa ttacttcaaa tggttagctt gccagctgac agtattaatc
    30961 gctaccctca cgagttctcc gggggacaaa ggcaaaggat tgttattgct cgtgcactgg
    31021 ttctcaaacc aaaaatttta gtttgcgatg agatcctttc agcgcttgat gtatcaactc
    31081 aggcccagat gcttgatttg cttcaggagc taaaaaaggg aatggggtta acctaccttt
    31141 ttatctccca cgacttatcg gtagtgcgga gtatttgttc aagggtaata gttttaaata
    31201 gaggcagtat cgaagaacaa gggcctgccg aggagatatt tagcaggccc caaagtgtat
    31261 atacgcagtt gcttatcgat gcaattcctg gtaaatcgct ttagtagatt tctatttcgt
    31321 ttatttcgta attagtttca ttttcacagt taatgctgat attgcccggc agtattggtc
    31381 tttgccaatc gtcgttgggc gatataccta gcgaggggtc agcataaacc ttttgcataa
    31441 acaaaccaaa gatagggaga gccatgttag ccccttgtcc taaggcgagg ttctggaagt
    31501 gaaccgaacg gtcctcggca ccagtccaaa ctcctgcaac aagatttggg gtaacgccca
    31561 taaaccaacc gtcagagtga tgctgggttg ttccggtttt tccacctatc tttccgggca
    31621 gctgatactt caaccgcaag cgaacccctg taccttgatt aacaacgctt tcaagcaggt
    31681 ttatcatgag gtatgctgtt tgctcactaa tggcttcgtt ttttttggtg ttaaaggttg
    31741 ccagcacatt tccgttttta tcctcaatgc gcgtaacaaa tattggttcc acctgaacac
    31801 ctttattcac ataagttcca tatgcaccta ccatttcata aaggctgaac tcaaatgtac
    31861 caagtattat tgagggaaca ggttcaatgt agctctttat accaagttga tgaatcattt
    31921 ccaccacagc gcttggcgca aactgcttca tgatccatgc agagatgttg ttaaccgagt
    31981 tggacaagcc ccattttagt gtgaccattt ggccatcgta cttggtgcta cccgagtttt
    32041 tgggcgccca aatggtatcg cccaccacaa aggtttgtgt aacattgggc accttggtgc
    32101 agggcgaata tccttcctgc atggctaagg tataaaggaa tggtttgatg gttgaaccaa
    32161 cctgtcgttt cccctgccga accatatcgt acttaaagtg cttaaaatca ggaccaccga
    32221 cgtaggcttt tacatgaccg ttatggggat ccattgccat aaaacttgaa cgcaggaatc
    32281 gcttgtagta ccttatggag tcaaggggcg acataagagt atcgcgctca cctttccatg
    32341 aaaacacccg catgggtatt ttctcattaa agttagccat tatgctatcg cgcgaagcac
    32401 ctgaggcaac aagaccccta tacctttgcg tttgccgcat tgcaaggtaa attatatggt
    32461 cggcctgttc gggagtaata tcgtaaaaaa ttcttcctcc gcgagcctta atctccttgt
    32521 caagtgctgg ttgaaggtcg tttttaagat gctcggcaag tgcctcttcg gcataccttt
    32581 gcatgcgaga gttaacggtg gtgtatatct tcagcccatc ggtgtaaaca ttgtaaggag
    32641 tgccatcggg tttggtgttc ttattgcacc acccgtaaag gggattagtt tcccacagca
    32701 gggaatcttc cctataaagc tgctgcgacc agtagcgctt gggattgggt tttgatgcag
    32761 tcatgaacaa tcgcaacatc tccctgaagt aggtagcagt tccctggtta tgatcctgtg
    32821 cttgatattt aagtacaatg ggcagttttg atatggaatc gaactgctct tgatttatgt
    32881 aaccgtattt aagcatttgc ccaagcacaa catttctgcg ttgttttgca ttttctgggt
    32941 tacgcactgg gctgtaccga gtaggtgcat taaccacacc cacaagtagg gcagcctgat
    33001 caatggttag tgagtcggga gttgtattaa aaaatgtttt agc
//
LOCUS       DAOOWJ010000017        32116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1420_length_32116_cov_8.254546, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000017 DAOOWJ010000000
VERSION     DAOOWJ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 32116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32116
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_32116_cov_8.254546"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..616
                     /locus_tag="PLW22_03120"
     CDS             59..616
                     /locus_tag="PLW22_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOU73726.1"
                     /translation="MSFFTITLLAIGLSFDTFAVSITCGIARKHIVFLEAFRVAAVFA
                     FFQSTMPLLGWGFGLAIKSVIEPIDHWLALALLCLIGVKMIVEAFGREENNSTLDPLN
                     PKVLFTLALATSIDAFAVGIGFVAVNVNMLHAYLIIGSVTFLVAMLGMLFGKSIGSVL
                     GKRMEIIGGLILIGLGVKIVIEHVS"
     gene            complement(625..1320)
                     /locus_tag="PLW22_03125"
     CDS             complement(625..1320)
                     /locus_tag="PLW22_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOU73727.1"
                     /translation="MSIALIKYLSEWGKRPSDLYKEPGPVVTISREMGCPGKQVSSTL
                     IEELNKRYRLRNELAWRWLAKEEILNIASTKLGLPKEDIDYVFEAKRKGIMQEILESM
                     STKYYKSDKHIQNTVKNIIRSEASKGHVVILGRGGVAITRDIPRSIHIHLEAPFEWRV
                     LRVEEMYHFEPREAELYVREIDRKREEIRVYFGGKDTDYTRFDITFNAMTLSVQEIVD
                     IIIHAMEVRNLIV"
     gene            complement(1431..3158)
                     /locus_tag="PLW22_03130"
     CDS             complement(1431..3158)
                     /locus_tag="PLW22_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73728.1"
                     /translation="MFGSFFFKKALLLLLFFPLPFSGYSATPFQEPQGVHELIKLANE
                     YERAGNANQAVYYYDKAANAYWQANNYQNAVVYFEKALENIIKVGNKNGEKLLYINLG
                     LVYSEISDYPQAQKCFESALSIATSSGKKGDVGQILYNISTVQIEQGNYQGALQNLAK
                     TEAIAQEVGDQKLLRNAYYNYNRAYEGLRNTEKAAEYFSLYAMLTKKIQAEEIRKKEL
                     QAKAMVDSAGRVVQQVSQEKEHAEKKLVETSKELKEKEQTLKEVEELTREQQMQIELL
                     NAEMKLRDAVIQNQKLLQKVYIGLILFSLAFAAALYYAYVQKRKANRLLKEKNDEISR
                     QRDAISRQADELRELNALKDKLFSIIAHDLRSPLFSLITMLNIAKEGHFAPENFKEIL
                     DELSVNVNHTTALLENLLTWAKTQMHGVKVNPQNFDLNQLVNSRIQLLNEAAENKEIK
                     LKNSIPDGTFVFADTDMVDIVIRNLISNAIKFCNAGDRITIWSTTGAERVTVCVEDTG
                     KGMTSDVLQKLFGTQITSTPGTKNEKGTGLGLILCKEFVQLNGGEIWAESQHDKGSKF
                     FFTLPKACV"
     gene            3414..3995
                     /locus_tag="PLW22_03135"
     CDS             3414..3995
                     /locus_tag="PLW22_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU73729.1"
                     /translation="MREITTLTEFTEQIKGKERVYLLVYKKGTGVSDCAFSAVKEAAE
                     ISKMENVFYVDAPTYPEIHKYYGVTSAPSLLVFEKGQLKDMVKGCHDTQFYKQLFENA
                     VFQQRVQNSTQQKSVTVYSTPTCPWCNTLKSFLRKNGIVFTDIDVSRDHEAARQMVSA
                     TGQQGVPQANIGGEWVVGFDQQKIKRLLNIQTQ"
     gene            4007..4294
                     /locus_tag="PLW22_03140"
     CDS             4007..4294
                     /locus_tag="PLW22_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOU73730.1"
                     /translation="MKELVPVNQHVVLDLSDDNTEQVTSSGIIIPDTAKEKPSWARVV
                     AVGNIENSEIAINDLVVFKKYSGTEVEFEGKTYLLIPYADLLAKVVETESI"
     gene            4682..6859
                     /locus_tag="PLW22_03145"
     CDS             4682..6859
                     /locus_tag="PLW22_03145"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOU73731.1"
                     /translation="MESKIFQVTGMSCAGCAISVETILKSQPGVLDAGVNFASSTVWV
                     NFDSTITSPESLRNAVRSIGYDLVIESVKHDIEAEKIKHINTLRNRFIYSLLLSIPLM
                     VVQMFFMHWSYTPIIALILSTPVIFWFGRQFHINASKQLKHFKANMDSLISISSIIAY
                     LYSIVAIVIKGNLQYRGITPHLYFESAAMIITFVLLGKWLEERAKGKTASAIKKIMGL
                     QPQMVWVKQNESIEEVPLTEVKKGMNVMVKPGERIPVDGIVTEGHSWVDESTITGEPI
                     PAEKSINQKVWAGTINLNGLLYIRAEQIGSESVLGKIIETVTNAQNSKAPVQRLADRV
                     AGIFVPIIIVISILTLAAWIVIAPGLAFSHGIIAAISVLAIACPCALGLATPTAVIVG
                     IGKAAKKNILVRDAESLEHACKIDTIIFDKTGTLTEGRPTVESINWFHHDDKYPSILG
                     MIEQHSNHPLAKSIVELIGASSRIFTIESFSEETGKGVKAVVDGKIYRVGSLEYLNQA
                     GVSDINSNQISINGMLIGFAEESRLLATVVITDKIKDTARSAINKLKNIGIEPIILSG
                     DNSKSVEIIAKEVGIERFERGMLPAGKKTYIENLKAQGKMVAMVGDGINDTEALSIAD
                     VSIAMGKGSDIAIDTSQVTILKTDLDAIPELITISKQTVKTIKQNLFWASIYNLISIP
                     IAAGVLFPFTGLLLDPMIAALAMAFSSVSVVTNSLILKAKRVG"
     gene            complement(6856..7398)
                     /gene="idi"
                     /locus_tag="PLW22_03150"
     CDS             complement(6856..7398)
                     /gene="idi"
                     /locus_tag="PLW22_03150"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOU73732.1"
                     /translation="MAEPRIALVDTNDNIIGYGEKQQVHFDGILHRAFSILICNAKGE
                     MLIHQRAHEKYHSPGLWTNACCSHLTESEQMETVINERLIHEMGFSCPLKHLFTFHYR
                     VEFDDGLIENEIDWVYLGRFDGSPNPNPDEVASWKWVSIDFLLADIENNPQSYTYWFR
                     HIMLEYKHRVLEAFEKCRVA"
     gene            complement(7404..8246)
                     /locus_tag="PLW22_03155"
     CDS             complement(7404..8246)
                     /locus_tag="PLW22_03155"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOU73733.1"
                     /translation="MVTVKAIYNPKRMTEGAGVSIQRILGFETRYECDPFLLLDFFGF
                     SSESEYKAGFPEHPHRGFETITYMLEGQVKHTDSTGGSGIIGPGEVQWMTAGSGIIHS
                     EMPTSDSGKMYGIQLWLNLPQEYKMVDPVYRELKREEIPVVETTSSQVKVISGKYRKR
                     DGGLQGLYKPVDIFDIKLWPNSAFDEVITPEFWNYKYFLFIYEGEFTVAHDLKPIKAP
                     CGVALSQGSIIQIRAGESGAKFLFFGAEPNSEPIAWHGPIVMNTDSEIQKALEELSNG
                     TFLK"
     gene            8418..11468
                     /locus_tag="PLW22_03160"
     CDS             8418..11468
                     /locus_tag="PLW22_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73734.1"
                     /translation="MMKRLLLLVYLIVFFFQIALIAQESSRFNSLNLTYEKGLDLYQK
                     EMYADAQKTFEQYIAGLPKGYYQQKVSAEFYRAMCAIFLNNDDAEYLIGQFIQNYPES
                     QLIDQAYFAMGKMQYQQKKYSQAVYWLSKIDKNGLDKDSRYEWQFMLGYGYFMTNDYE
                     SANRWLFQIKDIDNKFAAPATYYYSHIAYIQKKFATALKGFEKLQNDELFAQLVPYYI
                     THIYYLQGEYKKVTLYSPSLVRNNKNKRAPEVAKIVGDAFFKLKQYDSSLIYLKIYEE
                     KNKQQLSREDIYLLGFAYYKNSKYSEAAKYLERIATIDDSLSQNANYHLADCYLKLDD
                     KNKARQAFGLASKLDFDRIIKEDALFNFAKLTYEQLYAPFNEAIDAFKAYIKEFPSSP
                     RIDEAYNYLTLAYLSSRNYKEAVESLEKISKKDATINTALQRAAYFRGLELFQNLNYT
                     DAIQMFTKSLEYAGYNQQIAAQALFWRAESHYRLSNFENAANDLERFIVTPGAFGLEE
                     YLIAHYNLGYAYFKMKQYDTAITWFRKYNSLLNDSRVNTVADAYNRIGDCFFIQRRYW
                     AAIDYYEKAANLDVLDADYALFQRGFSLGLVSRPEKKIETLKGLLSKYPTSSYTDDAI
                     FEIAEAYNILENTSQAIDFYKRVESEFPNSSYYPKALVQIGLLYYNSSDLENSLAYYK
                     RVVETFPNSAEAKNALIGIRNIYIDKGDAETYFSYASKLGSIANISATEKDSLSYMAA
                     EKVYLTGNCEKSILAFENYLSENPQGNFAVNAMYYIADCNIRNNNVDKAVEYFENIIN
                     RPRNSFTEQSLLRIGNIYLNQKQYEKAYTYLDNLESIAEVKSMLLDARLGKLRCAFEL
                     QRDDQIPVIAANILSTEKLSPELERETRFKLAKANYRSKKYDDALIEFSRVAQNLKTI
                     EGAESKFMKALIYFERGEYNRTESEVFSFAENNTPHQYWLAKSFILLADSYAAQNDFF
                     QAKATLQSVLDGYSYTTDGIIDEATSKLNQLVKSEKERQSVKQD"
     gene            11479..13149
                     /locus_tag="PLW22_03165"
     CDS             11479..13149
                     /locus_tag="PLW22_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU73735.1"
                     /translation="MRSETKYIVIPLTAAMFLMAGSHAAAQTNDLKKEVQVVRPYEPS
                     ISDANKINLQPKIIDTIKVNPNFTYSIIQRPIETYFSPTPLSAARMVSEPLPDLHMSY
                     MRVGIGNTATPLAEAYLSSNRNKDYLYGAWVKFFNTSSKLELTNNLKVKAPASNIDIM
                     AFGKRMFNQKILLGDIGFTQNNRTYYGYFFENPALTPSNDKQSINRFAANLEFKTTNK
                     DSASINYHAQTNFYHLTDRYDLQETMVTGLFNLNKYFGQEQFGGEISFTHYGRSNPTG
                     SKNNALLQIKPWAHFFGDQWHAYAGLNIIYDANGNVNNTYFFPRAYLSYDIVSHYIIP
                     YVEIDGYVQENSYSHLLKQNPWLTSSIDTWNTVYKLIVGGGVKGKFSPTISYHVNANY
                     SIIDSLGLMVNTNSDNNNPLFNRFSLLYENTERTTVKGEIAFEPSKSFLVKLNGAFYQ
                     YKTQKISHPWHLPDYEITGFIQYAFRNKLKLNAEVFAVGKRWAKDPNDSPIRLKEYLD
                     INLGAEYVYNKRMSIFVYANNITGSKYQLWYLYPTQRFNMLAGVSYKF"
     gene            13369..15348
                     /gene="gyrB"
                     /locus_tag="PLW22_03170"
     CDS             13369..15348
                     /gene="gyrB"
                     /locus_tag="PLW22_03170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOU73736.1"
                     /translation="MSDKEKELKGENGSASYSASSIQVLEGLEAVRMRPAMYIGDTGN
                     KGLHHLVYEVVDNSIDEALAGYCNRIDLVINEDGSITISDNGRGIPVDFHEKEKKSAL
                     EVVLTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSVNLIAEVKRDGKIYRQEYSRGK
                     PTTELKVIGSTEETGTTITFMPDSQIFTQTTVFNFEILESRLRELAFLNKGIKLTLTD
                     KREIIENGTRSYRYAEFYSQDGLKEFIKFLDINREPLIENVIYIENDKGEVPVEVAMQ
                     YNTSFSENVHSYVNNINTIEGGTHLTGFRSALTRTLKKYADDSGMLSKLKFEISGDDF
                     REGLTAVISVKVAEPQFEGQTKTKLGNPEVRIAVDQAVSEALANYLEENPKDARNIVQ
                     KVILAAQARHAARKARELVQRKTVLSGSGLPGKLADCSDKDPAVSEIFLVEGDSAGGT
                     AKQGRDRRFQAILPLRGKILNVEKAMQHKIFENEEIKNFFTALGVSIGTEEDSKAINL
                     TGLRYNKIIIMTDADVDGSHIATLIMTFFFRYMTDLINKGHLYIATPPLYLVKKGKEE
                     RYCWNEEERLKAVEELGKGKDGSVHVQRYKGLGEMNAEQLWTTTMNPETRILRQVTIE
                     SAAEADRIFSMLMGDEVPPRREFIERHAKYANIDA"
     gene            complement(15374..16537)
                     /locus_tag="PLW22_03175"
     CDS             complement(15374..16537)
                     /locus_tag="PLW22_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOU73737.1"
                     /translation="MRLEAILFVVFFQLAGFLFAQSTFVFVERQAPNGMVKEKFQGVV
                     VNGDTLRQGRYLYFFDNGVLNQEGFYTNGKPDSVWITYYPSGARLSLFHYNSGKKDGP
                     FLFWNSDGTLYQQGYYKNDLLDGVLETYYPNGKLSAKSEYSKGRLNGISEVFDESGLL
                     RLRSGYKNDTLNGLWETYYDTGELNYKGTKCLGNYCDTLYTYYKNGVLMRKAYYSNGR
                     IDGVLISNYENGVLQIKAEYRNGSQDGNYTEYYPDGSIKETGCYFMNQKVGWWTSYYQ
                     GGQKHGQGNFSKGVLDGEWIFWWPNGNVKQHGKYCKGKACGRWKYFNSMGALIIDGNY
                     IDDKENGIWTTFYPNGAIAGRIWYLRGKPVSVKWLYDGQGLLQKRFSLEGLEK"
     gene            complement(16544..17395)
                     /locus_tag="PLW22_03180"
     CDS             complement(16544..17395)
                     /locus_tag="PLW22_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73738.1"
                     /translation="MLKRNLFYIIGGLLFSNLALGQDFEVAPVRLEFDAEPATSQMKT
                     ITVKNHSSRRVSYTVSFADFLPTTSGERKILPPNSTKRTCANWINVNPAFFELNPGED
                     IQIQVNMLVPGEEYGTAWCMLFIQPTREQTSWSADKQLAAGITVSGRIGVLVYQSPKS
                     NQNYSIKVSNFQEVTKPGDPQRKFSAVIENLGDKVAHCKVFLLASNMLTAEEKQFPPK
                     DFEIFPKLTRTVEMELPNDLPGGKYALAIIVDYGPKYPLEGSQLVIDIAGEIPKADKA
                     DSTKVNP"
     gene            17688..19556
                     /locus_tag="PLW22_03185"
     CDS             17688..19556
                     /locus_tag="PLW22_03185"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73739.1"
                     /translation="MKQIKLIFLLFYVMATAIHAVGQQEIVTIPKEKQDDIDRYKELV
                     VRYKQANNSQQAAFYLNKIAFIYWEFGNPKEAINYFLETIPLNEKVGNYNDIKAVYSN
                     IALIYSDMDRLDLTLEYFYKSLEARRKLNNKGEVAAGLIDVAYIHTALNQSEKAIQLL
                     EEALELSREVNNPRLILNSLKLLAQNYDNLGNKAKATEYGAMANAYEQQLATQQIKTE
                     YETKVVKSQAEAERERMQRTQQELLMSLRELQSKAMQDSLNFVVLRKQDSLRKAEEEA
                     QRKQTEIDLLNTDRMLKEAQLREREAQSRVQRIIILAGAIFVLVLIISSVAIYKNYTD
                     KKKANELLNLQNIEIQQQRDQIQKQNENISKSINYAQGIQRALLPPQSNLQAIFPESF
                     IFFRPRDVVSGDYYWFKELTTGYGSNEKTGKVAVSAIDCTGHGVPGAFLSMIGYNLLD
                     DIVFRGIHKPGAILTELNNGIRRTLRQDETDNRDGMDMALCVVDTKTNIVEFSGAKNP
                     LVYVVNNEVNRIRGDKESIGGGMDYRNDEFTTHVIKVESPTWFYMFSDGFIDQFGGPD
                     GRKYMIKNFIDLLAGISILPPDQQREILKNTLKDWLGTKYPQVDDILVVGFKIDPK"
     gene            19643..20422
                     /gene="dapF"
                     /locus_tag="PLW22_03190"
     CDS             19643..20422
                     /gene="dapF"
                     /locus_tag="PLW22_03190"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOU73740.1"
                     /translation="MMLQFFKYHGAGNDFIMIDNRDQAFTPMVQGVKHLCDRHYGIGA
                     DGLILLETQNGMPFMRYFNCDGNESTMCGNGGRCFATFAKKIGLANGLSFDFQGIDGM
                     HSAIFNTDGTVKLKMIDVETLEIIDDCYFINTGSPHYVTMVRDVNGVDVDREGRLIRQ
                     SVNFNNGGTNVNFIQEVAPGRIKIRTYERGVEAETLACGTGATASAIAYSHYFDINQT
                     PIEVEALGGLLKVYFDQIEEKYLNVWLEGPAEEVFEGKINY"
     gene            20504..20878
                     /locus_tag="PLW22_03195"
     CDS             20504..20878
                     /locus_tag="PLW22_03195"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU73741.1"
                     /translation="MKPLKLLETSDTPEVTLDKERGRFEFYGKIMPENPKEFFEPIIE
                     WFHEYLKEPNQETNLVFKLDYFNTAASKKIIEILSLLQNIKKENKAVQVNWYYKSIDE
                     DMLEVGETFSEIIHIPFNFYPY"
     gene            21055..22500
                     /locus_tag="PLW22_03200"
     CDS             21055..22500
                     /locus_tag="PLW22_03200"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOU73742.1"
                     /translation="MKAQKFILPVIYATLGGMLAIILSNHVLSSNNNEGVVTQKTPAQ
                     VVRAFAPDSSSTDFTWAAERSVDAVVHVTTEFTQSVDYSFGNPLFDFFFGPRDYMPRQ
                     KVQSSGSGVILTHDGFIVTNNHVIENADEITVVLNDKRKFTAKLVGTDPSTDIALLKI
                     DATDLPFLSFGNSDNIKIGEWVLAIGNPFNLTSTVTAGIVSAKARNINILSGREFAIE
                     SFIQTDAAVNPGNSGGALINLRGELIGINTAIASRTGSFTGYAFAIPSNIVKKVVTDI
                     IEFGEVQRAILGVRIQELTAELAKEKGIKEIKGVYIFDVEKGGAADAAGIKPGDVILS
                     VNGVDVNSTAQLQEQISRYRPNQQVDIVVNRDNSKKRFNVTLRNLKGGLGVVKTDDTL
                     SSLGAEFKEITDKQKQELGINYGLQIVELQDGKLKEGGIKKGYIITRMNRTPIRNIDD
                     LKRVLSISSGGVLIEGVYPNGVVAYYAIGVN"
     gene            22658..23518
                     /locus_tag="PLW22_03205"
     CDS             22658..23518
                     /locus_tag="PLW22_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU73743.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEDLITVEEEVELAQRIR
                     KGDQAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQEFERTPSPEELAD
                     LLELPKEKVNDTLRVSGRHVSVDAPFVDGEDNSLLDVLVNNDSPSADRGLINESLSRE
                     IERALATLTERERDIIKLFFGIGCQEMTLEEIGEKFGLTRERVRQIKEKAIRRLRHTS
                     RSKLLKSYLG"
     gene            complement(23567..24406)
                     /locus_tag="PLW22_03210"
     CDS             complement(23567..24406)
                     /locus_tag="PLW22_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOU73744.1"
                     /translation="MAYNLLKGKKGLIFGALNEKSIAWKVAERAFEEGAEVVLTNTPV
                     ALRFGNVYELAERMNTVVIPADATNIQDLENLIDQTMERFGGKIDFVLHSIGMSPNVR
                     KGRTYDDLDYEFFLKTLDISAISFHKILQTCWKKDAITHGGSVVALSYIAAQRTLYGY
                     NDMADAKALLESIARSFGYIYGRERGVRINTVSQSPTETTAGGGVMGFDALLDFSDRM
                     SPLGNASALDCADFCITLFSDLTRKITMQNIYNDGGFSSMGMSNRAMAVYNRNLEECK
                     DCK"
     gene            complement(24465..26135)
                     /gene="recN"
                     /locus_tag="PLW22_03215"
     CDS             complement(24465..26135)
                     /gene="recN"
                     /locus_tag="PLW22_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOU73745.1"
                     /translation="MIRKLYIQNYAIIDELEIEFKQGLNIITGETGAGKSILLGALSL
                     IQGQRADTSVLKHNDRSCIVEVEFDISEYNLNDFFEANDIDYDDITTIRRQISENGKS
                     RAFINEIPVNLNQLKELTEKLIDIHSQHQNLLLGNTRFQLDVLDAFAVSRDLLTDYRQ
                     LYDEFRKLKTELDRLEANAENAKRDFDYLSHQLNELNAANLRSGELDELEALQKQLTH
                     ASEIKTALEFSYGTLNADELAVLSKVKEIESNLRRIESVFSQANNLISRIESCRIELK
                     DIADEIDLLNSKVEVDDEQLHTVTQRIDLIYSLLNKHRLANFDELVEFRNQLENRVNE
                     IAQIDFNLDEKRKALRILEQKLQEKSKNLSQIRTSSAPKVEQFVTELLQQLGIKHAVF
                     RIRIEKLEEFQPWGTDRVTFYFSANKQIEPQELSRVASGGELSRLMLSLKSLLVKSTG
                     LPTIIFDEIDTGVSGEIADRMGTIIYELAKGMQVINITHLPQIAAKGTNHFLVYKSVT
                     NSQSSTGIKLLTPDERVVEIAKMLSGEKVTDAALMNAKELLKSNVSLS"
     gene            complement(26149..27060)
                     /locus_tag="PLW22_03220"
     CDS             complement(26149..27060)
                     /locus_tag="PLW22_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOU73746.1"
                     /translation="MNRFVGLLLVLLPFVANSQELRCNVSVNSQRIQGTNRLLFQNMQ
                     TAIYEFMNNTPWTNHVYGFDERIECSIMLNITEQIGSDEFKGTIQVQSRRPVFNTSYN
                     TTMLNFQDNNLHFRYREYEKLEFSESSHLSNLTSILAFYAYIIIGLDYDSFSKLGGTE
                     YFQKAERIVNNAQNAVERGWKAYEGNRKNRYWMIENLLNPKYQPLREALYRYHRLGLD
                     VMSEKAAEGRAEIAESLALLQRVYREKPDPYLFALQLFFDAKNEELVNIFSESYPTEK
                     ARVVNILTEIDNPNAAKYKRIIEQNTQ"
     gene            complement(27066..28274)
                     /gene="coaBC"
                     /locus_tag="PLW22_03225"
     CDS             complement(27066..28274)
                     /gene="coaBC"
                     /locus_tag="PLW22_03225"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOU73747.1"
                     /translation="MRLKGKHILIGVTGSIAAYKAATLIRLLVKEGAEVKVLMTPLAK
                     EFITPLTLATLSKNPILVDFFNPENGDWNSHVDLGLWSDLYIIAPASANTIAKMATGV
                     ADNLLLTTYLSARCPVMVAPAMDLDMYRHPATQQNISTLQARGNIIIEAATGELASGL
                     SGKGRMEEPENILEQVVSFFLTSQRLSGVRVLVTSGPTYEHIDPVRFIGNHSSGKMGN
                     AIALELARQGAIVDFVSGPVSQHPNHPNINIHRVTSAAEMYKAATTLFGEAKVAILAA
                     AVADFTVENVANQKIKREADDLVLRLKPTNDIAAHLGKVKKSNQLLVGFALETANEIE
                     NAKAKLQRKNLDMIVLNSMNDPGAGFGTDTNKVTFIMRSGQVVPFETKPKDMVAIDIV
                     DAVCEMLNKG"
     gene            complement(28264..28602)
                     /locus_tag="PLW22_03230"
     CDS             complement(28264..28602)
                     /locus_tag="PLW22_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOU73748.1"
                     /translation="MDIKKINAPVNTVTRDLDKLDRETGNIYQSVRIIAKRANKISID
                     MKQELNRKLEEFAYYTDNLEEVFENREQIEISKFYERLPKPTLIAAQEFLDGKLHYEF
                     KEDQTSESEA"
     gene            complement(28617..29432)
                     /gene="bamD"
                     /locus_tag="PLW22_03235"
     CDS             complement(28617..29432)
                     /gene="bamD"
                     /locus_tag="PLW22_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOU73749.1"
                     /translation="MKIQGLFKFERIVLLSFIIVLLSSCSGFEKVLKSKDYEYKYKMA
                     LEYYKKKDHYRYVTLLEQLQSVYKGTFRADTIDFYLAQGYYHQGDYLLAGHQFDMFRK
                     NYQRSVFTEEAEFMYAYCYYMSSPRPELDQSNTLAAIEAFTEFINRYPNTSHKAEVNK
                     IMVELRDKLVEKSYLSAMLYYKIGDYKAAIVAIRNSIRDYPESKHREELMFHLLKASY
                     LLADNSIESKRRERFQNALDEYYSFISEYPSSKYSSEAKKMYDATMKVLQNLN"
     gene            29693..30931
                     /locus_tag="PLW22_03240"
     CDS             29693..30931
                     /locus_tag="PLW22_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU73750.1"
                     /translation="MDIFDKINNMGPIGQWQQYSHGYFSFPKLEGEIKPRMIFRGKEV
                     LTWSLNNYLGLANHPEVRKADAEAAAKYGMAYPMGARMMSGQTTKHEELEQKLAKFVG
                     KESAYLLNYGYQGMVSIIDTMLNRNDVVVYDSESHACIIDGLRLFPGKRFVYQHNDMD
                     SCRKMLQHATKTAEKTGGGILLITEGVFGMAGDLGNLKAIVDMKKEFNFRILVDDAHG
                     FGTMGKTGAGTPEHFGVQNEIDLYFATFAKSMAGIGGFVASNEKVIRYLAYNMRSQIF
                     AKSLPMPMVFGALKRLELLQTQPELKENLWKVVKALQSGLKEMGFDIGKTESPVTPVY
                     MKGGIPEAANMVFDLRENYGIFCSMVVYPVIPKGQILLRLIPTAVHTLDDVKETINAF
                     AAVREKLLAGKYDKEKIAQI"
     gene            complement(31002..31961)
                     /gene="trxB"
                     /locus_tag="PLW22_03245"
     CDS             complement(31002..31961)
                     /gene="trxB"
                     /locus_tag="PLW22_03245"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOU73751.1"
                     /translation="MESAGKELPVENVRVLILGSGPAGYTAAIYAARANLKPVLYEGI
                     QPGGQLTTTTEIENFPGYPEGITGTEMMEDLKKQAMRFGTDVRFGIATSTDLSKQPYR
                     VVIDDEKVIETDALIIATGATAKYLGLESEEKFKGMGVSACATCDGFFYRGKDVAVVG
                     GGDTACEEATYLAGLCNKVYMIVRKPFLRASKAMQDRVFNTPNITVMFEHVTKEIVGD
                     QSGVNGVILTNTKTGEDVKIDISGFFLAIGHHPNSDIFKPWIETDEVGYIKTIPGSTK
                     TNLPGVFACGDVQDPHYRQAITAAGSGCMAAIDAERYLGERNF"
BASE COUNT         9613 a         6831 c         6432 g         9240 t
ORIGIN      
        1 ccccccatat ccaaattctc ccctattatt tttatttttg tcaaaatttt gaattatcat
       61 gtctttcttt accattactc ttctagcaat tggtctttcc ttcgatacct ttgcagtatc
      121 aatcacatgc ggaatagccc ggaaacatat tgtatttttg gaggccttta gggttgcagc
      181 agtatttgct ttttttcagt caacaatgcc gcttttgggc tggggtttcg ggttagccat
      241 aaaatcggtt attgagccta tcgatcactg gttagcgctg gcattgcttt gccttatcgg
      301 cgtaaaaatg attgttgagg cattcggaag ggaggagaac aacagcactc ttgatccact
      361 aaacccaaaa gtacttttta cccttgcatt agcaaccagt atcgatgctt ttgcagtagg
      421 gatagggttt gtagcggtaa atgtaaacat gctgcatgca taccttataa ttggctccgt
      481 tacttttttg gtagccatgc ttggtatgct tttcggaaaa agtattggca gcgtgttggg
      541 taaaagaatg gagataatag gtgggcttat actaattggg ctgggagtaa agatcgttat
      601 tgaacacgtt agctaaggac tttattaaac gataaggtta cgcacctcca tggcatggat
      661 tataatatca acaatctcct gcaccgatag ggtcatggca ttaaaggtga tatcaaagcg
      721 ggtataatca gtgtccttcc cgccaaaata aaccctaatc tcttcgcgct tacggtcaat
      781 ttcacggaca tacagttcag cctccctggg ttcaaaatgg tacatttcct caacccgcaa
      841 cactcgccat tcaaaagggg cttcaaggtg tatatgaatt gaacgaggaa tatcacgggt
      901 tattgctaca ccgcctcttc ccagaataac aacatgccct tttgaggctt cggatcgaat
      961 aatgtttttt actgtattct gaatgtgttt atcgctttta tagtattttg ttgacattga
     1021 ctcgagtatc tcctgcatta tccccttgcg ctttgcttca aacacatagt caatatcctc
     1081 ctttggtaaa ccaagtttag tagaagctat attgagaatc tcctccttgg ctaaccaacg
     1141 ccaggctaac tcatttctca acctgtacct cttatttagt tcctcaatta gggttgaaga
     1201 aacctgtttc cctgggcagc ccatttcgcg cgaaatggtg acaaccggcc ctggctcctt
     1261 gtacaaatcg cttggtcgtt ttccccattc cgataaatat tttataaggg ctatactcat
     1321 agaattttct atttgttcta aagatataaa aataaaggtt taaagtcaat aaaaaagggg
     1381 ttagaaaaac ctaaccccaa atgagtggtc aagaagaaat tgaacagtcc ttaaacacag
     1441 gctttaggca aagtgaagaa aaacttactt cccttatcgt gctggctttc agcccaaatc
     1501 tcacccccat tcaattgaac aaactcttta cacaggatca gtcccaatcc cgttcccttt
     1561 tcattcttag ttcctggcgt tgaagtaatt tgggtaccaa atagcttttg aagtacatcg
     1621 gaagtcatgc ccttacctgt atcctcaaca catacagtta ccctttcggc gcctgtggta
     1681 ctccatatcg ttatcctatc gccggcattg cagaatttta tggcattgga aatcaggtta
     1741 cgaattacaa tatcaaccat atcggtatcg gcaaaaacaa aggtaccatc cggaatactg
     1801 ttctttaact tgatctcctt attttcagca gcctcgttca atagctgaat acgagaattc
     1861 accaactgat ttaagtcgaa gttctgagga ttaaccttta ctccatgcat ttgcgtttta
     1921 gcccaggtta agagattctc aagcagtgct gtagtatggt taacgtttac tgagagttcg
     1981 tcgagtattt ccttgaaatt ttcaggagca aaatgacctt ccttagcaat attcagcatg
     2041 gtgattaacg agaaaagcgg gcttctaagg tcgtgggcaa taattgaaaa cagtttatct
     2101 ttgagagcat tcagctctct gagctcatcg gcctgacggc taatagcatc gcgctgtctg
     2161 gaaatttcat cattcttctc tttaagtaat ctatttgcct tgcgtttctg aacgtatgcg
     2221 tagtagaggg ccgctgcaaa tgccaaggag aataggatta aaccaatgta aaccttctga
     2281 agcaactttt ggttttgaat tactgcatcg cgtagcttca tctcagcatt caaaagttca
     2341 atctgcattt gctgctcacg ggttaactct tcaacctctt tgagggtttg ctctttttcc
     2401 tttagctcct tgctagtttc aacaagtttc ttttcagcat gctccttttc ctgtgatacc
     2461 tgctgtacaa ccctaccggc actgtcaacc attgcttttg cttgcaactc tttctttctt
     2521 atttcctcgg cttgaatttt cttggttagc atggcataca ggctaaaata ttcagcagcc
     2581 ttttccgtat tcctcaaacc ctcataagcc ctgttgtagt tataatatgc attacgtagc
     2641 aatttttgat cgccaacctc ctgggctata gcttcggttt tagcaagatt ttgcaatgcg
     2701 ccctgatagt taccctgctc aatctgaacc gttgagatat tataaagtat ttgaccaacg
     2761 tcgccttttt tcccgcttga agtggcaata cttagggcac tctcaaaaca tttttgagct
     2821 tgggggtaat ctgaaatttc ggagtaaact aatccaagat tgatatataa aagcttttcg
     2881 ccatttttat tcccaacttt aataatattc tctagagctt tctcaaaata aactactgcg
     2941 ttttggtagt tatttgcttg ccagtaggca tttgctgcct tatcgtagta gtaaaccgct
     3001 tggtttgcat tcccagctct ttcgtattca ttggctagtt taataagctc atgtacacct
     3061 tgaggttcct gaaacggtgt agcggaataa cctgaaaaag gcaagggaaa aaacagtaat
     3121 agtagtaatg cttttttaaa aaaaaatgac ccaaacataa tttatccttt taaacaaact
     3181 gaaattttta ctgaatcata cgtgaactaa ccttttaacg atacgttatt ctccaatttt
     3241 tctgtgaata gaacttaagt gttggcaaat ggctttattt gtgttgtaaa tttatatttc
     3301 aaaataatga caaattttca aaatcagcaa acttgtcaca ttaaaaattg acatatttac
     3361 attgttgtat atttggcttg aaatttgttt atgaccatta aaaatagata gttatgcgcg
     3421 aaataaccac actaacggaa tttactgagc aaatcaaggg aaaggaaagg gtttaccttt
     3481 tagtttacaa gaagggaaca ggggttagcg actgtgcatt cagcgcagtt aaagaagccg
     3541 ccgaaatttc caaaatggaa aatgtatttt atgttgatgc cccaacctac cctgaaattc
     3601 acaagtacta tggggtaacc agcgctccat cgctgttggt ttttgaaaag ggtcaactta
     3661 aagatatggt aaagggctgt cacgacacac agttctacaa acagcttttt gagaatgccg
     3721 tcttccagca aagggtccaa aactcaactc agcaaaagag cgtaacggtc tattctactc
     3781 caacctgccc atggtgcaac acccttaaaa gttttctccg taagaatggt atagttttta
     3841 ctgacatcga tgtgtcgcgc gaccatgagg ctgctcgcca aatggtttca gcaacaggcc
     3901 aacagggtgt tcctcaagcc aacattggcg gtgaatgggt ggtaggcttc gaccagcaaa
     3961 aaattaaacg actacttaat attcaaaccc aataaattag ttcgatatga aagaattagt
     4021 gccagttaac cagcatgttg tgctcgacct gagcgacgat aacactgaac aggttacatc
     4081 atcgggtatc attattcccg atacagccaa ggaaaaacca tcgtgggcaa gggtagttgc
     4141 cgtaggcaac atcgagaact ccgaaattgc tattaacgat ctggtggttt ttaagaagta
     4201 ttcaggaacc gaggttgaat tcgaaggaaa aacttacctc ctaatacctt acgccgattt
     4261 gctggcaaag gttgtggaaa ctgaatcaat ttaactctcg tattctgact ttcctaagtt
     4321 tttgcacgcc accgcttggc gtgcttttta cttttttaaa atgctctgct ttaaacccaa
     4381 gtactatgaa ttaatcgttt ttctcactat tcctctaact tcctctaact tcctctaact
     4441 tcctctaact tcttctaact tcttctaact tcttctaact tcttctaact tcctcacatt
     4501 ccgactcatt ccgtctcatt ccgattcatt ccgactcatt ccgactcatt ccgactcatt
     4561 cctataaatt catatcactt cggttaaacc tattcaatat gagagttgtt ttttatcaat
     4621 agcagtaaac cattttttga gagtttactc acatactttt acatatttat tttttactaa
     4681 catggaaagc aaaatatttc aggttacagg catgtcgtgt gctggctgtg caataagcgt
     4741 ggaaaccatc ctcaaaagcc aaccgggagt gttggatgct ggggttaact ttgccagcag
     4801 tacggtttgg gtaaactttg acagtacaat cacatcccct gaaagtctcc gcaatgctgt
     4861 tcgttcaatt ggttacgact tggtgattga aagcgtaaag catgacatag aggcagaaaa
     4921 gataaagcac atcaacaccc tcagaaaccg gtttatttac tcactattac tctcgattcc
     4981 cttaatggtt gtgcaaatgt tttttatgca ttggagttac acaccaataa ttgcattgat
     5041 actttcaact cctgtaattt tttggtttgg aaggcaattc catataaatg ctagcaagca
     5101 acttaaacat tttaaagcaa acatggatag cctcatttcc attagctcaa taattgcata
     5161 tttgtatagc attgtagcca ttgtaataaa aggtaattta caatacaggg gcattacgcc
     5221 acacctttac tttgaatcgg cagccatgat aatcaccttt gttttacttg ggaaatggct
     5281 ggaggagaga gcaaaaggca aaacggcttc agccatcaaa aaaataatgg gacttcaacc
     5341 ccaaatggta tgggtaaagc aaaacgaaag tattgaagag gttccattaa ccgaggtaaa
     5401 aaaaggaatg aatgtaatgg taaagcctgg cgagcgtatc cctgttgatg gtattgtaac
     5461 tgaagggcat agctgggtag atgagagcac aattaccggt gaaccgatac ctgctgaaaa
     5521 aagcatcaat caaaaagttt gggctggaac tattaatcta aatggtttgc tatacattag
     5581 ggctgaacag attggttctg agtcggtttt gggcaaaatc atcgaaactg taactaacgc
     5641 tcaaaattca aaagctccag ttcagaggtt agccgatagg gttgcaggaa tttttgtgcc
     5701 tattatcatc gtaataagta ttctaacact tgctgcatgg atagtaattg ctccaggttt
     5761 agcgttcagt catggaatta ttgctgccat ttcggtttta gctatagcat gcccttgtgc
     5821 acttggtttg gcaactccaa cagcagtaat agttggtatt ggcaaggctg caaaaaaaaa
     5881 cattctggtt cgcgatgccg aaagccttga gcacgcgtgc aaaattgata ccattatttt
     5941 cgataaaaca ggcacgctta ccgaaggtag gcctaccgtt gaatccatta attggttcca
     6001 ccacgatgat aaatacccta gcatacttgg catgattgag cagcactcca atcatcccct
     6061 tgcaaaatca attgttgagc tcattggtgc aagcagtaga atttttacca ttgaatcttt
     6121 ttctgaagaa accggaaaag gagtaaaggc agtagttgat ggtaaaattt atcgcgtggg
     6181 aagtttggaa tacttgaacc aagccggggt atctgatatt aattcaaacc aaataagtat
     6241 caatggcatg cttattggtt ttgctgaaga aagccggttg ttagccactg tagttattac
     6301 tgataaaata aaggatacag cgcgatcagc aattaataag ttaaagaata ttggcattga
     6361 gccaattata ctatcgggcg ataactcaaa aagcgttgag ataattgcaa aagaggttgg
     6421 cattgaaagg ttcgaaaggg gaatgctacc tgccggtaaa aaaacctaca ttgaaaacct
     6481 gaaagcccaa ggcaaaatgg tagccatggt tggcgatgga ataaacgata ccgaggcgct
     6541 aagcatagcc gatgttagca ttgctatggg taaaggatcg gacattgcta ttgatacatc
     6601 gcaggtaaca atcctcaaga ctgacttaga tgcaatacct gaactaatta caatttcgaa
     6661 acaaaccgta aaaacaatta agcaaaacct tttttgggca tcaatttata acctcatcag
     6721 tattcccata gctgcaggtg ttttattccc atttacagga ttacttctcg atccaatgat
     6781 tgcagccctc gccatggctt ttagttcagt atcggttgta actaacagtc tgatactaaa
     6841 agctaaacga gtaggttaag caaccctgca cttttcaaag gcctcaagca ctctgtgctt
     6901 atattccagc atgatatgcc tgaaccagta ggtgtagctc tgcgggttgt tttcaatgtc
     6961 tgctaacaaa aagtcaatgc taacccattt ccagctggcc acttcatcgg gatttggatt
     7021 tggactacca tcgaaacggc ccaaataaac ccaatcaatt tcgttttcaa ttaaaccgtc
     7081 atcgaattcc accctgtaat gaaaagtgaa tagatgcttc agaggacatg aaaagcccat
     7141 ttcatgtatt aaacgttcat taataactgt ttccatctgt tcgctttcgg ttagatggct
     7201 gcagcaagca tttgtccaca atccaggtga gtggtatttc tcgtgtgccc tttggtgaat
     7261 aagcatctca ccctttgcgt tgcaaattag aatggaaaag gctctgtgta gaattccatc
     7321 gaaatgaact tgctgtttct caccataccc aataatattg tcattggtat caactaatgc
     7381 aattcgtggt tctgccataa atgctatttc aaaaaagttc cgttactgag ttcctctaac
     7441 gctttttgga tttcggagtc ggtattcatc acaatggggc catgccaggc aatgggctca
     7501 ctattaggct cggcgccaaa gaacagaaat ttagctccgc tttcacctgc cctaatttga
     7561 attattgaac cctgcgatag ggcaacacca caaggagcct ttattggttt tagatcatgt
     7621 gcaactgtaa actctccttc ataaataaac agaaagtact tataattcca aaactcagga
     7681 gtaataactt catcgaatgc ggaattgggc caaagcttga tatcaaaaat atcaacaggc
     7741 ttgtaaaggc cctgcaatcc cccatcgcgt ttgcgatact tcccggaaat aactttaacc
     7801 tgagaggagg ttgtttctac aacaggtatc tcctcccgct ttagttcacg gtatacagga
     7861 tcaaccattt tatactcctg tggcagattt agccagagct ggatgccata catctttcca
     7921 ctatcgcttg ttggcatctc gctatggatt atgccacttc ctgcagtcat ccactgaacc
     7981 tcacccgggc caattattcc actacctcca gtgctatcgg tatgctttac ttggccttca
     8041 agcatgtatg taatggtttc aaacccccta tggggatgct cagggaagcc tgccttatat
     8101 tccgattccg acgagaaacc aaagaagtcc agcagcagaa agggatcgca ctcgtatctg
     8161 gtttcaaaac ccaatatcct ttgaatactt accccagctc cttcggtcat tcgtttggga
     8221 ttgtagatgg cttttactgt taccattata tgtataggtt gttacatctg taaatataac
     8281 actttttaga ataccgttgt ttaatagttt actcaacttt taataaatta ttttaattgt
     8341 tgattaaaag ttggctcaac aataaatgtt taggttaata gttactattt ttaggcctga
     8401 ttaatatatt atatcgaatg atgaaaaggt tattactcct ggtttacctc atcgttttct
     8461 tttttcaaat tgcgcttata gcgcaggaga gcagtaggtt taactcttta aacctgacct
     8521 acgaaaaggg tcttgaccta taccaaaagg aaatgtatgc cgatgctcaa aaaacatttg
     8581 agcaatacat tgcaggtttg cctaaaggtt actaccagca aaaggtttca gcggagttct
     8641 acagggcaat gtgtgcaatt tttctgaata acgatgatgc tgaatatctt atagggcaat
     8701 ttattcaaaa ctaccctgaa agtcagctta ttgatcaggc atactttgcc atgggaaaaa
     8761 tgcaatacca acaaaaaaag tattcgcaag cggtgtactg gttatcaaaa attgataaga
     8821 acggcctaga caaagattct cgctatgagt ggcagtttat gttaggatac ggctatttca
     8881 tgactaacga ttacgaatcg gccaatagat ggctattcca gataaaagat atcgataata
     8941 agtttgcagc accagcaacc tactattact cacacattgc ttacattcaa aagaaattcg
     9001 ctacagccct aaaaggtttt gagaaactac agaacgatga gctttttgct caacttgttc
     9061 cttactacat cactcatata tactacctac agggcgaata taaaaaggtt accttgtatt
     9121 ccccatcctt agttaggaat aataaaaaca aacgtgcacc tgaagttgcc aaaattgttg
     9181 gcgatgcctt ttttaagctt aaacagtacg atagctcact aatctacctt aagatttacg
     9241 aggaaaaaaa caaacagcaa ctctcacgtg aggatattta ccttttggga tttgcctact
     9301 acaaaaacag caagtattcc gaagcagcaa agtacttgga acgtattgcc accattgacg
     9361 actcgcttag ccaaaacgct aactaccact tagccgactg ctaccttaag cttgacgata
     9421 agaataaggc tcgccaagca tttggccttg cctcaaaact tgattttgat agaattatca
     9481 aggaggatgc actatttaac ttcgctaagc taacatacga acagctttac gcgcctttca
     9541 atgaagcaat cgatgccttt aaggcataca ttaaagagtt ccccagctca cccaggattg
     9601 atgaggctta taactaccta acacttgcct acttaagttc acgaaactac aaggaggcag
     9661 tggaatcgtt ggaaaaaatc agtaagaaag acgcaaccat aaatactgcg ttgcagcgtg
     9721 cagcatactt ccgcggactt gaacttttcc aaaatcttaa ctacaccgat gccatacaaa
     9781 tgtttacaaa atcgcttgaa tatgccggtt ataaccaaca aattgcagca caagcgctat
     9841 tctggagagc tgagtcgcat taccgcctat caaactttga aaacgcagcc aacgaccttg
     9901 aaaggtttat agttacccct ggggcttttg gtctggaaga gtatcttatt gcccactata
     9961 acctgggcta tgcatacttt aaaatgaaac agtacgatac agccattact tggttccgta
    10021 agtataatag cttactaaac gattcacgag taaacacggt tgccgacgct tacaacagga
    10081 ttggtgactg tttctttatc caacgacgct attgggctgc aattgattac tatgaaaaag
    10141 ccgcaaacct tgatgtgctc gatgccgact acgccctatt ccaaagaggt ttttcattgg
    10201 gtttggttag ccgtccggag aaaaagatag aaacattaaa ggggcttctt agcaaatacc
    10261 caacatcaag ctacaccgat gacgctattt ttgaaatagc agaggcttat aacattctgg
    10321 aaaatacgag ccaggcaatt gatttctata agcgagttga gtcggaattc cccaacagca
    10381 gttactaccc aaaagcttta gttcaaattg ggctgcttta ctacaactca agcgatttgg
    10441 aaaactcact cgcatactac aagagggttg tggagacctt tcccaattcg gctgaagcca
    10501 aaaatgccct tattggtatt agaaacattt atattgataa aggcgatgcc gaaacctact
    10561 ttagctatgc atccaaactt ggatcaattg ccaatataag tgctactgag aaagactcgc
    10621 taagctacat ggccgctgaa aaagtttacc tcacaggcaa ctgcgaaaaa tcaatactag
    10681 catttgagaa ctacctcagc gaaaaccctc aaggaaactt tgctgtaaat gcaatgtact
    10741 acatagccga ctgcaatatt cggaataaca atgttgataa ggcagtggaa tactttgaaa
    10801 acattattaa taggccccgt aacagcttca ccgaacaaag cttgctaagg attggcaata
    10861 tctacctaaa ccaaaaacag tacgaaaaag cttacaccta ccttgataac ttagagtcga
    10921 tagctgaagt taagagcatg ctactcgatg cccgtttagg caaactacgc tgtgcatttg
    10981 agctgcaacg cgacgaccaa atacccgtga ttgcggcaaa catattgagc accgagaagc
    11041 tatcgcctga gctagagcgt gaaacccgtt ttaaactagc caaagccaac tatcgttcca
    11101 aaaagtacga cgatgcgctt attgagtttt caagggttgc acagaacctt aagactattg
    11161 agggcgctga atccaagttc atgaaggctc taatttactt tgaacgcggc gagtacaata
    11221 gaactgaaag cgaagttttc tcgtttgcag agaataatac tccccaccag tactggttgg
    11281 caaaaagctt cattttgctt gccgactcgt atgccgctca aaatgacttt ttccaagcca
    11341 aagctaccct ccaaagcgtt ctagatggct actcgtacac aaccgatgga ataatcgacg
    11401 aggccacctc aaagctaaac cagttagtaa aatccgaaaa ggaacgtcaa tctgtaaaac
    11461 aagattaatt tcacagcaat gagaagcgaa acaaaataca tagtaatacc cctaactgca
    11521 gcaatgtttt taatggctgg tagccatgcc gctgcgcaaa ccaacgatct aaaaaaggaa
    11581 gttcaggttg ttcgccccta tgaaccatca atatccgatg caaataagat taatctgcaa
    11641 cccaagatta ttgacaccat aaaggtaaac cccaacttta cttactccat aattcaacgc
    11701 cccattgaaa cttacttctc tcctactccc ctatcggcgg cccgtatggt ttcggagcca
    11761 ctacccgatt tgcatatgag ctacatgcga gtaggaatag gaaacacagc caccccacta
    11821 gctgaggcct acctcagcag taaccgaaac aaggattacc tttacggtgc ttgggtaaaa
    11881 ttttttaata ctagctccaa acttgaactt accaacaacc taaaggttaa agcccctgca
    11941 tcaaatatcg atatcatggc atttggtaag agaatgttta accaaaaaat tttactgggc
    12001 gacatcggtt ttacccaaaa taaccgaact tactatggtt acttttttga aaatcctgca
    12061 cttacacctt ccaacgacaa gcaaagcata aataggtttg ctgcaaatct tgaatttaaa
    12121 accacaaaca aagattcagc tagcataaat taccatgctc aaaccaactt ctaccattta
    12181 accgataggt acgaccttca ggaaacaatg gttactggac tctttaacct caacaaatac
    12241 tttggacagg aacagtttgg cggcgaaatt tcgtttaccc attacggtcg ctccaatcca
    12301 acaggttcaa agaataacgc acttttgcag attaaaccct gggcccattt ctttggcgac
    12361 caatggcatg cctatgccgg tttaaacatt atttacgatg ccaatggaaa tgtaaacaac
    12421 acctacttct tccctcgcgc ttacctctcg tacgatattg ttagccatta cattataccc
    12481 tacgttgaaa tcgatggcta tgttcaggag aactcatact ctcatcttct aaaacaaaac
    12541 ccatggttga cctcaagcat tgatacatgg aatacagtat ataagctcat tgtaggcgga
    12601 ggggttaagg gtaaattcag ccctaccatt tcgtatcatg ttaatgctaa ctactccatt
    12661 attgatagtt tgggactaat ggttaacacc aactccgata acaataaccc cttatttaac
    12721 cgattcagct tattatacga aaatacggag cgaactacgg ttaaaggaga aatagccttt
    12781 gaaccatcaa aatcgtttct agtaaaactt aatggagcat tttaccagta caaaacgcaa
    12841 aagatatcac acccctggca cttgcccgat tatgaaataa ccggttttat ccagtatgca
    12901 ttccggaata agctgaaatt gaatgccgag gtatttgctg taggtaagcg ctgggcaaaa
    12961 gaccctaacg atagcccaat taggcttaaa gaatacctgg atatcaacct tggtgctgag
    13021 tatgtttaca ataaaaggat gtcgattttt gtatacgcaa ataatataac gggatcaaaa
    13081 taccagctct ggtatctata cccaacccag aggttcaata tgctggctgg agtaagttac
    13141 aaattttagt gatattgcta attatccaga ataatcaaaa agacatcttc tgcttatacc
    13201 caaatatgta gggtttgata tcaattttaa aaaatatcaa catcacgcgt aattaattaa
    13261 atatcaatca gttgatcaaa taaaaaagat acaaaaccat taagataaac caaatatttt
    13321 ggttatcttt gaaggttaaa tttaaagttg atttagtgct attctgctat gagcgataag
    13381 gaaaaagaat taaagggtga aaatggtagt gctagttact cagccagtag cattcaagtt
    13441 ctggaaggat tagaggcggt gcgcatgcgc ccggccatgt atattggcga cactggtaat
    13501 aaaggattac accacttggt ttacgaggtg gttgacaact ctattgacga agcacttgca
    13561 ggctactgta atcgcatcga tttagttatt aacgaagatg ggtcaataac tatttccgat
    13621 aacggacgtg gaattccggt tgacttccac gaaaaggaaa agaaatcggc actggaggtg
    13681 gtgcttacgg ttctgcatgc cggtggtaag ttcgataagg actcatacaa ggtttccggt
    13741 ggattgcatg gtgtaggtgt atcgtgcgtt aatgcactct cagtaaacct aattgctgag
    13801 gtcaaacgcg atggaaagat atacaggcag gagtattccc gcggtaagcc aacaacggag
    13861 cttaaggtta ttggaagcac tgaggaaacc ggtacaacta taaccttcat gcccgatagc
    13921 cagatattca ctcaaaccac tgtgtttaac tttgaaattc tggaatcgcg gctaagagaa
    13981 ctagcattcc ttaataaagg cattaagcta acacttaccg ataaacgcga gataattgaa
    14041 aacggtaccc gtagctaccg ttacgctgaa ttctactcac aggatggttt aaaggaattc
    14101 atcaaatttt tggatataaa ccgtgagcct ttaattgaaa atgttattta cattgagaac
    14161 gacaaaggtg aagttcctgt tgaggttgcc atgcaataca acacttcatt ttccgaaaac
    14221 gttcactcat acgtaaataa catcaacaca attgaaggtg gcacccacct tacgggattc
    14281 agaagtgcac ttacccgcac actaaaaaaa tatgccgacg actcaggaat gttgagcaag
    14341 ctcaaattcg aaatcagcgg cgacgatttt cgcgagggat taaccgccgt tatctctgtt
    14401 aaggtggccg aaccccaatt tgagggtcaa accaaaacca agctgggaaa ccccgaggtt
    14461 cgaatagcgg ttgaccaggc ggtaagcgaa gccttggcca actacctgga ggaaaaccct
    14521 aaggacgcaa ggaacattgt tcaaaaggta attctcgccg ctcaggctcg tcatgcagca
    14581 cgtaaagcac gtgagctggt tcagcgtaaa accgttcttt caggatctgg ccttccgggt
    14641 aaacttgccg actgctccga taaggaccct gctgtatcag agattttcct tgttgagggt
    14701 gattcggcag gaggtacagc aaaacaaggg cgcgataggc gtttccaggc aattctaccg
    14761 ctacgtggta aaatcctgaa cgtggaaaaa gccatgcaac acaaaatatt cgaaaacgaa
    14821 gaaattaaga atttttttac tgcattaggt gtgtcaattg gaaccgagga ggatagtaag
    14881 gccattaatc ttacagggtt acgttacaac aagattatta tcatgaccga tgccgatgtt
    14941 gatggtagcc acattgcaac actcattatg actttctttt tccgctatat gaccgatttg
    15001 ataaacaagg gtcacctcta tattgcaaca cctcctctat accttgtaaa gaagggtaag
    15061 gaagagcgtt attgctggaa cgaagaggaa cggctgaaag cagttgaaga gcttggtaag
    15121 gggaaggatg gatcagtgca tgttcaacgc tataaaggtt tgggcgagat gaacgccgaa
    15181 cagctttgga ctacaaccat gaaccctgaa acccgaatac tccgtcaggt aaccattgaa
    15241 agtgccgctg aagctgatag gattttctcc atgctcatgg gcgatgaagt tccccctagg
    15301 agggagttta ttgaacgtca tgccaaatat gcaaatatcg atgcgtagta gaaaaggccg
    15361 gatttccggc cttttatttt tctaaccctt caaggctaaa tcgtttctgc agcaaacctt
    15421 ggccatcgta aagccacttt accgataccg gtttccccct caaataccat atcctaccgg
    15481 caattgctcc attgggatag aatgttgtcc aaatcccatt ttctttatca tctatgtaat
    15541 tcccatcaat aataagcgct cccatactgt taaaatattt ccacctaccg caagcttttc
    15601 ccttacagta tttaccatgt tgtttcacat ttccgtttgg ccaccaaaaa atccattcgc
    15661 catctaaaac cccttttgaa aaattgcctt ggccatgctt ttgtccaccc tgatagtagc
    15721 ttgtccacca tcccaccttt tggttcataa aatagcatcc ggtttctttt atactaccat
    15781 cggggtaata ctcagtataa ttcccatcct gagaaccatt tcgatactct gccttaatct
    15841 gcaatacacc attctcataa ttcgaaatca aaacaccatc tatcctacca ttactatagt
    15901 aggcctttcg cattagtact ccatttttat agtatgtgta aagcgtatcg cagtaattac
    15961 ccaaacattt agtgccctta tagttaagct cgccagtatc gtaataggtt tcccagagtc
    16021 cgttaagggt atcgttttta tatccactac gcagacgtag taatccggac tcgtcgaaaa
    16081 cctcacttat cccatttagc ctacccttac tatattctga ttttgcgcta agctttccgt
    16141 taggatagta ggtctcaagc actccatcca aaaggtcatt cttgtagtac ccctgctggt
    16201 aaagcgtacc atcggaattc cagaagagga aagggccatc ctttttgcca ctgttataat
    16261 ggaataggct taacctagca ccacttgggt agtaagtaat ccaaacacta tcgggttttc
    16321 cgtttgtata gaaaccctct tgatttaaaa ctccgttatc aaaaaagtaa agataacggc
    16381 cttgtcgtaa ggtatcgcca ttaactacaa ctccctgaaa cttttcttta accatcccat
    16441 ttggagcttg cctttcaaca aaaacaaacg ttgactgggc aaataaaaag cccgccagct
    16501 gaaaaaatac tacaaataga atggcttcta acctcaaggg taattatgga tttactttag
    16561 tactatcagc tttatctgct ttaggtatct cgccagctat atcaataaca agctgcgaac
    16621 cttccagtgg gtattttggg ccatagtcaa caattattgc aagggcatac ttgcccccgg
    16681 gcaaatcatt tggcaactcc atttccacag tcctagttaa cttaggaaaa atttcaaaat
    16741 cctttggagg aaattgtttt tcctcagcgg ttagcatatt cgatgccaaa aggaaaacct
    16801 tacagtgagc aaccttatcg ccaaggtttt caattactgc agaaaatttt ctttgagggt
    16861 cgcctggctt agttacttct tgaaagttgc taaccttaat ggagtagttt tgatttgact
    16921 tgggcgactg gtaaaccaat acaccaattc ttccgcttac agttattcct gcagcaagct
    16981 gcttatcggc actccaactg gtttgctcac gggttggttg tataaaaagc atacaccatg
    17041 cagttccgta ttcctcgcca ggaactagca tattaacctg aatctgaata tcctcgcccg
    17101 gattcaactc aaaaaaagcc gggttaacgt taatccagtt tgcgcaggtg cgcttggttg
    17161 aattaggagg tagtattttc cgctcaccgc tagttgttgg gagaaaatca gcgaacgaaa
    17221 ctgtatatga tactctccta ctgctatgat tctttacggt aatagttttc atctggcttg
    17281 tagcgggttc tgcatcaaac tccaaacgca ccggcgcaac ctcaaaatct tgacctaagg
    17341 caagattaga aaatagtaac ccgccaataa tgtaaaacag gtttcgctta agcatacttt
    17401 tttaaccttt taaatatttt ccaattttca aaaataaatg ataaaagcaa aatattttcc
    17461 ataaaaatat taaaaatatc gataaataga aggtaaccgt atttaaaaaa attgcattta
    17521 tcatggttat acagcttaag cagagcaaag ggcattttaa aaagttaata tttatcctaa
    17581 ggctaatcag caacttagtt aactcatgtt tggattaaat aaaatacatg gttgtttttt
    17641 gaacaatgaa aaaaattaag tatgtttgag taaaatataa gtttaggttg aaacagatta
    17701 aactgatttt tttactattt tatgtgatgg ccactgctat tcatgcagtt ggtcagcagg
    17761 agattgttac aatcccaaaa gaaaaacagg atgatattga tagatacaag gagttagtag
    17821 ttcgatacaa gcaggcaaac aactcacagc aagcagcatt ttatctgaat aaaatagcat
    17881 ttatttattg ggagtttggt aatcctaaag aggctattaa ctattttctt gaaaccattc
    17941 cgctcaacga aaaggttgga aactataacg atattaaggc tgtttacagc aacatagctc
    18001 ttatatactc tgacatggac aggcttgatc ttacccttga gtacttttac aagagccttg
    18061 aagcccgcag aaaacttaat aataaaggag aggttgctgc cggtttaatt gatgttgcat
    18121 acattcacac agcccttaac caatccgaaa aagccattca actgcttgag gaggcacttg
    18181 aactttccag ggaagtaaac aatccccgct taatccttaa ctcgcttaaa ctgcttgccc
    18241 aaaactacga taacctagga aacaaggcca aagcaactga gtatggcgct atggctaacg
    18301 cctatgaaca acaacttgcc acccagcaaa taaaaactga atacgagaca aaggttgtaa
    18361 aatcgcaggc cgaagcagaa agggaacgca tgcagcgcac tcaacaggaa ctccttatga
    18421 gtttgcgcga gttgcaatca aaagccatgc aagactccct taactttgta gttctacgta
    18481 agcaggatag ccttcgcaag gcagaggagg aagcccaacg caagcaaacc gaaattgatc
    18541 tgcttaatac cgatagaatg ctcaaggagg cgcagttgag ggagcgagag gctcaatcca
    18601 gagttcaaag gataatcatt ctggccggtg caatatttgt tttagtactg attatctcat
    18661 cggttgccat ctacaagaat tacacagaca aaaagaaagc gaacgaactg cttaacctgc
    18721 aaaatattga aatacaacag caacgcgacc aaatccagaa acagaacgag aacataagta
    18781 aaagtattaa ctacgcacaa ggcattcaaa gggccttact ccctccacaa tccaacctac
    18841 aggctatttt ccctgaatcg ttcatatttt tccgtccacg cgatgtggtg agtggcgact
    18901 actactggtt caaggagtta accaccggat acggatccaa tgaaaaaacg ggtaaagttg
    18961 ccgtttcggc aattgactgt actggacacg gtgttcccgg tgcgttcctg tccatgattg
    19021 gttacaacct gctcgatgat attgtattcc gtggaattca taaaccagga gccattctaa
    19081 ccgaacttaa caatggtatc cgaagaactc tcaggcagga tgaaaccgat aaccgtgatg
    19141 gtatggatat ggccctttgc gttgttgata ccaaaactaa cattgttgaa ttctctggag
    19201 ctaaaaaccc cttggtttat gtggttaaca acgaggtgaa ccgtattaga ggcgataaag
    19261 aatcaattgg aggcggaatg gattaccgta acgatgagtt caccacccac gtaattaagg
    19321 ttgaaagccc tacatggttc tatatgttct ccgatgggtt tatcgatcaa tttgggggcc
    19381 ccgatggacg aaaatatatg attaaaaatt ttattgacct tctggctggc atatccattc
    19441 tgccccccga tcagcagcgc gagattctta aaaacacatt aaaggattgg ttgggaacaa
    19501 aatatccgca ggttgacgat atcctagtgg ttggctttaa aatcgatcct aaataatttt
    19561 taatttttct cctattaatt tccaatttta ttgggttctt cgttattttt cccgaatatt
    19621 gtagggtatt attacatgat ttatgatgct acagtttttt aaataccatg gtgctggtaa
    19681 tgactttata atgattgaca atcgtgacca agcttttaca ccaatggttc aaggtgttaa
    19741 acacctttgc gacagacatt atggaattgg agccgacggt ctgatattgc tcgaaaccca
    19801 gaatggtatg ccattcatgc gttactttaa ttgcgatggt aacgagtcga ccatgtgcgg
    19861 gaatggggga agatgctttg ccacttttgc caagaaaatt gggctggcca acggattatc
    19921 gttcgacttt caagggattg atgggatgca ctcggcaatt ttcaataccg atggcacagt
    19981 aaagctcaaa atgattgatg tagaaaccct agaaataatt gacgactgtt actttatcaa
    20041 tacaggctcc ccgcactatg tgaccatggt tagggatgtt aatggcgttg atgtggaccg
    20101 cgagggccgc ctcataaggc aaagcgtgaa tttcaacaat ggaggaacca atgtgaattt
    20161 tattcaggaa gtagctcctg gtagaataaa aatccgaacc tacgagcggg gtgtagaggc
    20221 tgaaacccta gcctgcggaa ctggtgcaac agcatcagcc atagcatact ctcactactt
    20281 cgatatcaac caaaccccaa ttgaggttga agcccttggc ggtttactca aggtctattt
    20341 tgatcaaatt gaagaaaaat acctgaacgt ttggctcgaa gggcctgccg aagaagtttt
    20401 tgagggcaaa attaattact aaaacaaaat tgcattaatt ccattttttc ttaattttgt
    20461 taacagtcct tgctttgtgt tactttacta aaagttgaaa aagatgaagc cattaaagtt
    20521 gttggaaacc agcgataccc cagaggtcac actggataag gagaggggcc ggtttgagtt
    20581 ctatggaaaa attatgcccg agaaccccaa ggagttcttt gagcctataa ttgaatggtt
    20641 tcatgagtat ttaaaggagc caaatcaaga aactaatctg gttttcaagc tcgactactt
    20701 taacactgct gcctctaaaa agattattga aattctctca ttactacaaa atattaaaaa
    20761 agaaaataaa gccgtacagg taaactggta ttacaaaagt attgatgagg atatgcttga
    20821 ggttggtgaa accttctcgg aaataattca tatacccttt aacttttacc cgtactaagt
    20881 atttcgcatg atgccaaaaa ttcagaatgt gatttagctc acatttaatt ttgtcgtggc
    20941 tttattttaa aaacatgaaa aattgacata cctctggtta acatttgtta atcagctgca
    21001 caaggtattt tttttgttac ctaaattagc agaaaccata aaacaagtta gactatgaaa
    21061 gcacagaaat ttatactacc agtaatttat gccactcttg gtggcatgct ggccatcatt
    21121 ttaagcaacc atgtattgag ttcgaataat aatgagggag ttgttaccca aaaaactccc
    21181 gctcaggtag ttagagcgtt tgcacccgat tcctcttcta ctgactttac ctgggctgca
    21241 gagcgatcgg ttgatgcagt agtacacgta acaacagagt tcacccaatc tgttgactac
    21301 tcgttcggga accccttatt tgacttcttt tttgggccac gcgactatat gccgcgccaa
    21361 aaggttcaga gctctggctc tggagttatt ttaacccacg atggtttcat tgttaccaat
    21421 aaccatgtga ttgaaaatgc cgatgaaatt actgtagtgc tgaacgacaa gcgtaagttt
    21481 accgcaaaac tagtggggac tgatccaagt accgatattg cgcttctaaa gatagatgct
    21541 accgatctgc ccttcctatc ctttggcaac tccgataaca ttaaaattgg cgaatgggta
    21601 ttagccattg gaaatccctt taacctaaca tcaacggtta ctgccggaat tgtaagtgcc
    21661 aaagcacgaa atatcaacat acttagtgga cgcgaatttg ccatagaatc gtttattcaa
    21721 accgatgctg cagttaaccc cggaaacagc ggcggtgctt taattaacct taggggtgaa
    21781 ctcattggta ttaatacagc cattgcttca agaacgggtt cttttactgg ttatgcattt
    21841 gcaataccct caaacattgt taaaaaggtt gtgaccgata tcattgaatt tggtgaggta
    21901 caacgggcca ttctgggtgt tagaatccaa gaacttaccg cagagctagc caaggaaaaa
    21961 ggtataaagg aaattaaagg agtttacata tttgatgttg aaaagggtgg cgccgctgat
    22021 gctgctggta ttaaacccgg cgatgtaatc ctctcggtta atggcgttga tgttaacagc
    22081 actgctcagc tacaggaaca aatcagccgt taccgtccta atcagcaagt tgacattgtg
    22141 gtaaatcgcg ataattcaaa gaagcgattc aatgtaacac tccgcaacct taaaggtggt
    22201 ttgggtgtag taaaaaccga tgatacgtta agtagcttag gggcagagtt taaagaaatc
    22261 accgataagc aaaagcaaga gcttggaatt aattacggtt tgcaaatagt tgaactccag
    22321 gatggtaaac ttaaggaagg cggaataaag aaaggataca ttattactcg tatgaaccgg
    22381 acaccaattc gcaatatcga tgacttaaag cgtgtactaa gcatttcatc gggtggtgtt
    22441 ttaattgagg gggtttaccc caatggtgta gttgcctact atgcaattgg cgtaaactaa
    22501 aaattatgag attttgacat attaaatgaa actttttaaa aatagcaacg ttttaactta
    22561 acacgttaga acgttgcttg tttattttgt ttaacatatt ttttttcttg atattaacta
    22621 gtgtttgtag taattttgca ttaaatatac gggtcggatg agacaattaa agatcacaaa
    22681 atcaatcact aacagggaga gcgcatcgct cgataagtat ttacaagaga ttggtaagga
    22741 agaccttatc actgtagaag aggaagttga gcttgcccag cgtattcgca aaggcgatca
    22801 ggcagcgctg gagaaattga cacgtgccaa tttgcgtttt gtggtttcgg tagccaagca
    22861 gtaccaaaac cagggactta gcctacccga cctaattaac gagggtaacc tagggcttat
    22921 taaagctgct gaaaaatttg atgaaacccg tggtttcaaa ttcatttcct atgctgtttg
    22981 gtggattcgt caatccatac tgcaagcatt agctgaacaa tcgcgcattg ttcgcctacc
    23041 gcttaaccag gtaggatcgc taaacaaaat taacaaagca ttctcaaagt ttgagcagga
    23101 gtttgaacgt acaccttcgc ccgaggagtt agctgattta cttgaactac ccaaggaaaa
    23161 ggtaaatgat acgctccgcg taagtggccg ccacgtatcg gttgacgctc cctttgttga
    23221 tggtgaggac aatagccttt tggacgttct tgtcaacaac gattcgccaa gcgccgaccg
    23281 tggtttaata aacgaatcgt taagccgcga aatagaacgc gctcttgcca cactcacaga
    23341 gcgagaaagg gacatcatta aacttttctt cgggataggt tgccaggaga tgacccttga
    23401 agaaattggc gaaaaatttg gcttaacccg tgaacgtgta cgtcagataa aagaaaaggc
    23461 tatccgccgt ttgcgccaca cttcacgtag taaattacta aaatcgtatc ttggttaaca
    23521 aaaagcccgc caattggcgg gcttttcaat tatttatcca aatacattac ttacagtcct
    23581 tgcactcctc aaggtttcgg ttgtaaacag ccatggccct gttgctcata cccatgctag
    23641 agaatcctcc atcattataa atgttttgca tggtgatttt gcgggttaaa tcgctgaata
    23701 gggttataca aaaatcggca caatcgagcg cactggcatt acccaagggc gacatacgat
    23761 cagagaaatc gagcagggca tcaaaaccca ttactccacc acctgcggtg gtttcagttg
    23821 gtgactgcga taccgtgttg atacgaacac ctcgttcccg gccatagata tacccaaagc
    23881 tccgtgcaat agactcaagc agggcttttg catcggccat atcgttgtaa ccgtaaaggg
    23941 tgcgttgtgc tgcgatatag gaaagggcta ctaccgaacc gccatgggta atagcatcct
    24001 ttttccagca ggtttgcaaa attttatgga atgaaatggc tgaaatatcc agtgtcttaa
    24061 ggaaaaattc gtaatccaaa tcgtcataag ttctgccctt cctaacgtta ggcgacatcc
    24121 cgatggaatg gagcacaaaa tcaattttac caccaaaacg ctccatggtt tggtcaataa
    24181 ggttctccag gtcttgaata tttgtggcat cggcgggaat tacaaccgtg ttcattcgct
    24241 cagcaagctc atatacattg ccaaagcgaa gtgctactgg ggtattggtt aaaacaacct
    24301 cagcgccctc ctcaaaagcg cgctctgcca ctttccacgc aatggatttt tcgtttaggg
    24361 caccaaaaat aagtcccttc ttacctttca gtaaattgta agccataaat acatcgttta
    24421 attgaactaa caaagataaa caaagaaacc ggaaattggt tcaattagct taaactaacg
    24481 tttgatttaa gcagttcctt tgcattcatg agtgcagcat cggtaacctt ctccccactt
    24541 agcattttag ctatttccac taccctttca tcgggggtaa gaagctttat acctgtagag
    24601 ctttggctgt tggttaccga cttgtaaacc aagaagtggt tggtgccctt tgcagcaatt
    24661 tgcggtaagt gggtgatatt gattacctgc atacctttag ccaactcgta tatgatggtt
    24721 cccatcctat cagctatctc gccagaaacg cctgtgtcaa tctcatcaaa aattatggtt
    24781 ggtaaaccgg ttgattttac cagtaacgat tttaggctaa gcattaacct ggaaagctcg
    24841 ccacccgatg caaccctcga tagttcctgg ggctcgattt gcttattggc tgaaaagtaa
    24901 aaggtgaccc tatcggtacc ccagggttgg aactcttcca gtttttcgat acgaatccta
    24961 aaaacagcgt gtttgattcc cagctgctga agcaattctg ttacgaactg ctcaacctta
    25021 ggcgctgagc ttgttctaat ctgactaagg ttctttgact tttcctgaag cttttgctct
    25081 aagatccgga gcgcttttct cttttcatcc aggttgaagt caatttgtgc aatttcattt
    25141 actctatttt caagctgatt tcggaattca actagctcat caaagttagc caaacggtgc
    25201 ttgttaagta aagagtaaat aagatcgata cgttgggtta cggtatgtaa ctgttcatcg
    25261 tcaacttcaa cctttgagtt gagcaagtca atttcatcag caatatcctt aagctctata
    25321 cggcaactct caatccgaga tattaggtta ttagcctggg agaatacaga ttcaatgcgc
    25381 cgcaggttgg actcaatctc ctttactttt gataaaactg caagttcgtc ggcattaagg
    25441 gttccatagg agaactcgag agctgtttta atttccgatg catgcgtaag ctgtttctga
    25501 agtgcttcca gctcatcgag ttcgccgcta cgcaggttag cagcatttag ctcattgagc
    25561 tgatgggata ggtaatcgaa atcgcgcttt gcattttcag cgttagcctc taacctatcg
    25621 agctcagttt taagctttcg gaactcatcg tacagctgac ggtaatcggt aagtaaatcc
    25681 cttgatacag caaaggcatc aagcacatcg agctgaaaac gggtgttacc cagtaaaagg
    25741 ttctgatgct gggagtggat atcgatgagc ttttcggtaa gctccttgag ctggttaaga
    25801 ttaaccggaa tctcgttgat aaatgcacgt gatttaccat tctcacttat ctggcgacga
    25861 atggtggtaa tatcatcata atcaatatcg tttgcctcaa aaaaatcatt taggttatac
    25921 tctgaaatgt caaactcaac ctcaacaatg cacgacctat cgttatgttt gagtaccgat
    25981 gtatcggctc gctggccttg tatcaaggaa agtgcaccta acaatatcga tttaccggca
    26041 ccggtttcac cggtaattat atttaaccct tgcttgaact ctatttcaag ctcatcaatt
    26101 atagcatagt tctgtatgta gagtttacga atcatgctat tatcggtgtt actgtgtatt
    26161 ctgttctatt atacgcttat actttgcagc gttggggttg tcaatttctg taaggatatt
    26221 taccacacga gctttttcgg ttgggtacga ttcagaaaaa atattgacaa gctcctcgtt
    26281 tttagcatca aaaaacagct gcagggcgaa aagataggga tcgggtttct cgcggtaaac
    26341 tcgctgaagc aaagcaagcg attcggcaat ctcagccctt ccctcagctg ccttttcgct
    26401 catcacatct aatcccaagc ggtggtagcg ataaagggct tcgcgcaagg gttgatactt
    26461 tgggtttagg agattctcaa tcatccagta cctattcttt ctgtttccct cataggcttt
    26521 ccagcctcgt tcaacagcat tttgagcatt atttacaatg cgttctgctt tctgaaagta
    26581 ctcagttcct cctagttttg aaaatgaatc gtagtccagc cctataatga tatatgcgta
    26641 aaacgcaagg atggaagtta agttgctgag gtgcgatgat tctgaaaact ctagcttctc
    26701 gtactcgcga taccgaaagt gaaggttatt atcctgaaaa ttgagcatcg tagtgttgta
    26761 cgatgtatta aataccggtc gacgtgattg tacctgaatg gttcccttaa actcatcgga
    26821 gcctatctgc tcggttatgt tgagcatgat gctacactca atacgctcat caaatccgta
    26881 aacatggttg gtccacggag tgttgttcat gaactcgtaa atggcagttt gcatgttctg
    26941 gaatagtagc ctgttggtac cctgaatcct ttgagagttt acggagacat tgcatcgcag
    27001 ttcctgcgag tttgccacaa agggtaataa tactaaaagt aagccaacaa acctattcat
    27061 gcaaatcagc ctttattcaa catttcgcat actgcatcaa caatatcgat tgcaaccata
    27121 tccttgggtt ttgtttcaaa gggaactacc tgaccactgc gcataataaa ggttacctta
    27181 ttggtatcgg ttccaaaacc tgcaccaggg tcattcatgg agtttagaac aatcatatcg
    27241 agatttttcc gttgcagctt tgcctttgca ttctcaatct cgttagctgt ttccagtgca
    27301 aaaccaacta ggagttgatt gctcttcttt actttaccaa ggtgggcagc aatatcgttg
    27361 gtgggtttaa gcctaagaac aaggtcatcg gcctcgcgct taattttttg attggcaaca
    27421 ttttcaactg tgaaatcggc tacggcagca gcaagaatgg caaccttagc ctcgccaaaa
    27481 agggtggtag ctgccttgta catttctgct gcactagtta ccctgtgtat gttgatattg
    27541 gggtggttgg ggtgttgcga aacaggtcca cttacaaaat caacaatagc accctgtcga
    27601 gccagctcaa gggcaatggc attacccatc ttgccactgg agtggttacc aataaaccga
    27661 acgggatcaa tatgctcgta ggttggcccc gaagttacta atacccgaac tccactaagg
    27721 cgctgactgg ttaaaaaaaa acttacaacc tgctcaagaa tattctcggg ttcttccatt
    27781 ctacctttcc cactcaggcc gctagcaagc tctcctgttg ccgcttcaat aataatattc
    27841 cctcgcgcct gaagtgttga gatattctgt tgagttgctg ggtgcctata catatccaaa
    27901 tccattgcgg gagctaccat aaccgggcaa cgggccgaaa gatatgttgt aagcaacaga
    27961 ttatcggcta ctccggtagc cattttagca atggtattgg ccgaagccgg cgcaatgata
    28021 tataaatcgg accaaaggcc taaatccaca tgactattcc aatcgccatt ttcaggattg
    28081 aaaaaatcaa ccagaatagg attttttgag agagttgcca acgtaagggg agtaataaac
    28141 tccttggcca ggggtgtcat taacacttta acctcggcac cctcctttac aagcaaacga
    28201 ataagcgttg cagccttata ggctgcaatg cttccggtaa caccgattag aatatgtttt
    28261 cctttaagcc tcactctcgg aagtttgatc ttccttaaac tcatagtgca gctttccatc
    28321 cagaaactct tgtgctgcta tgagtgtagg tttgggaaga cgctcataga actttgaaat
    28381 ctcaatttgc tcacggtttt caaaaacctc ttcaaggtta tcggtatagt aagcaaattc
    28441 ctcgagctta cggttaagtt cctgtttcat atcaatgcta atcttattgg cacgtttggc
    28501 aattatccga accgattggt agatattgcc tgtttcccta tccaacttat ccaaatcgcg
    28561 ggttactgtg ttaactggtg cattgatttt cttgatgtcc atagcgatat ttatacttag
    28621 tttaaatttt gcaacacttt catagtagca tcgtacattt tttttgcctc gctcgagtac
    28681 tttgaacttg ggtactcact gataaaggag tagtactcat caagcgcatt ctgaaaacgt
    28741 tcccttcgct tgctctcaat actattatcg gctaaaaggt acgatgcttt aagtagatga
    28801 aacattagct cctcgcggtg cttactttca gggtagtccc ttatactatt tcgaattgcc
    28861 acaatagccg ccttgtaatc gccaatcttg tagtaaagca ttgcgctaag gtacgatttt
    28921 tcaaccagct tatcgcgcaa ttcaaccatt atcttattca cctcggcctt atgagaggta
    28981 ttggggtagc gatttataaa ttcagtaaaa gcctcaatgg ccgcaagggt attggattga
    29041 tccagctcag gccttggcga cgacatgtag tagcagtaag catacatgaa ttcagcctcc
    29101 tcggtgaaaa cgctgcgctg atagttttta cggaacatat caaactgatg acctgcaagc
    29161 aggtagtcgc cctggtggta gtaaccttga gccaaataaa aatcaatggt gtcggcacgg
    29221 aatgtaccct tatacacgct ttgaagctgc tcaagtaggg ttacataacg gtaatgatct
    29281 ttctttttgt agtactccaa ggccatcttg tatttatact catagtcctt gctcttaaga
    29341 accttttcaa atccactaca cgacgataaa agaacaataa taaaggaaag caatacaatt
    29401 ctttcaaact taaaaaggcc ctgaattttc ataatcaaac caattattat ttaatgcttt
    29461 gttttaagca ggcaaattta ttcaaaatct ttgtgaaaat taatgttttt ggaattacat
    29521 gactaaaaaa ttcatgccat tcgatcataa aatttgttaa tacacagtat tacagatatt
    29581 tagcaaatga tgtgaataaa ttttataacc aacaaacgca ttgatagcaa atttagtata
    29641 gatttgctga gaatttgagg taacgaatta ctaaccaaaa ttttttataa ccgtggatat
    29701 ttttgataag attaacaaca tgggcccaat aggccaatgg cagcagtata gccatggcta
    29761 tttttcattt ccaaaacttg agggcgaaat taaaccccgc atgattttcc gcgggaaaga
    29821 ggttttaaca tggagtttga acaactacct gggtttagcc aaccatcccg aagttcgcaa
    29881 agccgatgct gaggctgccg caaagtatgg catggcttac ccgatgggag cccgcatgat
    29941 gagcggtcaa accaccaagc acgaggaact tgagcaaaaa cttgcaaagt ttgtgggcaa
    30001 ggaatcagct tacctgctaa actacggcta ccagggtatg gtttccatta ttgataccat
    30061 gcttaaccgt aacgatgtgg ttgtatacga ttccgagtcg catgcttgta ttattgatgg
    30121 gcttcgcctt ttcccaggta aacggtttgt ttaccaacat aacgacatgg atagctgccg
    30181 caaaatgttg cagcatgcaa ccaaaaccgc agagaaaacc ggtggcggaa ttctcctgat
    30241 taccgaaggc gtatttggca tggccggcga cctaggtaac cttaaggcta ttgttgacat
    30301 gaagaaggag ttcaacttcc gcattttggt tgacgatgcg catggctttg gaaccatggg
    30361 taaaaccggt gccggtactc ctgaacactt tggcgtacag aacgaaattg acctttactt
    30421 tgcaactttt gccaagagca tggcaggcat tggcggtttt gtagcatcga atgaaaaagt
    30481 gatacgttac ctagcctaca acatgcgttc gcaaatcttt gccaagtcgt tacccatgcc
    30541 tatggtattt ggagcactta agcgtttgga actactacaa acccaaccag aactaaagga
    30601 aaacctttgg aaggtagtta aagcccttca atcgggatta aaggaaatgg gatttgatat
    30661 aggtaaaact gaatcgccag taacccccgt ttacatgaaa ggtggaatac ccgaagcagc
    30721 aaacatggtt ttcgatttgc gtgagaacta tggcattttc tgctccatgg tggtttaccc
    30781 tgtgattcca aaaggacaaa tcctgctccg acttatacct acggccgtac ataccctgga
    30841 cgatgttaag gaaacaataa acgcctttgc agccgtaagg gaaaaactgc tggcaggaaa
    30901 gtacgataag gaaaagattg ctcagatata gtagtaaaca gaaattcaaa acaaaacccc
    30961 gttttaacgg ggtttttgtt tttattgaaa tcaaagaggc attaaaagtt tcgctcccca
    31021 agataccgtt cagcatcaat ggcagccatg caacccgaac cggcagcagt aatagcttga
    31081 cggtaatgcg gatcctgtac atcgccgcag gcaaatacac caggaagatt tgttttggta
    31141 cttccgggta ttgtttttat atatcccacc tcatcggttt caatccaggg tttgaatata
    31201 tcggagttag ggtgatggcc aatggccaag aaaaatccgg aaatatcaat ttttacatcc
    31261 tcgccggttt ttgtgttggt aagtattact ccgtttaccc cactttggtc acccacaatc
    31321 tcttttgtta cgtgctcaaa cataacggtg atgttgggtg tgttgaatac cctatcctgc
    31381 attgcctttg atgctcttag gaagggttta cggacaatca tgtaaacctt gttacacagt
    31441 ccagccaggt aggtagcctc ttcacaggcc gtatcgccac ccccaactac agcaacgtcc
    31501 tttccacggt aaaagaaccc atcgcatgtg gcacaagccg aaacccccat gcccttgaac
    31561 ttttcttccg attcaaggcc aaggtacttt gctgttgctc ctgttgcaat gattagagca
    31621 tcggtttcaa ttaccttctc atcatctata acaaccctgt agggttgctt ggatagatcg
    31681 gtggaagtag ctataccaaa cctcacatcg gtaccaaaac gcattgcctg ttttttgagg
    31741 tcttccatca tttcggtacc agtaataccc tcggggtaac cggggaaatt ttcaatttca
    31801 gttgtggtag ttagctgtcc gccgggttgg ataccctcgt atagaacagg cttgaggttg
    31861 gctcttgctg catatattgc agcagtatag cctgcaggcc ctgaaccaag aatcagaacc
    31921 cgcacattct caacaggcag ttccttgcct gctgattcca tagcatcatc ggtgcttagt
    31981 gatttaaata tcgccatagc tatatatatt tattaattta gatatttcaa tttgatgcaa
    32041 aaatactaat aatttgcaat gcctaaacaa ttatttaaat tgattttaaa caatattttt
    32101 tgatgaataa agatag
//
LOCUS       DAOOWJ010000018        31973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1427_length_31973_cov_8.406260, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000018 DAOOWJ010000000
VERSION     DAOOWJ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 31973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31973
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1427_length_31973_cov_8.406260"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..3466
                     /locus_tag="PLW22_03250"
     CDS             26..3466
                     /locus_tag="PLW22_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU73752.1"
                     /translation="MNNSKPNPEEFYRLLAQSKERLKELNAINSVINITKENRPIPET
                     LHRVCFVLPDAWQYPEHTVARIKYGQYEFQTFGFKETPWCQRQGFESIDGVFGAIEIF
                     YTKQFPDEFEGPFLKEERDLLNNIANIIEGYINSIKGRDVLKEKTQAKRKPDLAEPQS
                     KRLLQRFINQHNADRDIYHDLMPFKVHEILLISTLYDAYSIEREDRLTDNILGEYSKL
                     SLSSVPRITGVSTLDEALEKLEERYFNLIIIMMGADTVTPLRMASRIKGEYKHIPLYL
                     LVNNSIIVNEIEKNPNTIEGVDRIFVWNGEPKIFFSMIKLLEDRANIENDTRIALTRV
                     ILLVEDSPKYYSRYLPLLYGSVLDQTKRVIEDVSTDDLYKVLRIRIRPKILLAGTYEE
                     AIDLFTRYKDYMLCMISDVKFFRDGRLDENAGVRLVEHARKMLPDLPIILQSFEKSNE
                     AIAHKLKVAFLDKNSESLLNDIKSFLSNYLGFGDFVFKDEAGNPIAIATTMEEFERAL
                     RIIPDESLLYHAQKNHFSMWLSARGEIQVARIIHPSKIDDFSGPAEIREYLLSTLKKY
                     RQEKRRGKIVGFDTNWEVDSSNIVSLADGSVGGKGRGLAFINTLLYTFDISQYTPNIN
                     LLTPRTSIIGTNEFECFMARNGLYDKVFACKSFEELQRHFIKAELSDQLKLRLDRLLQ
                     IYYRPLAVRSSGMLEDSIMQPFAGVFETYLIPNAHPDRSVRLQRLMNAIKLVYASVFS
                     PTARAYIKAMNLRVEDEKMAVIIQEVVGNKFENYYYPHISGVAQSHNYYPFGHINPDD
                     GFANIAIGLGKHVVEGGRSYRFCPKYPTLVNYTMEDLIKNSQVDFLAVDLSKCDYDLL
                     TGDEAGLARLDLYEAERHGTLKHCASVYSSENSSLIPGIGQSGPRVVNFANILKYNYV
                     PLAQTIDVILDIVEEALGAPCEIEFAVDLNRDANFRSSFFLLQIKPLMASAQEYRLDI
                     NTIDRAEAILLSNNAMGNGHLTNIEDVVYIRREAFNKAMTLEMANEVEWLNNTLEDEG
                     RKYILIGPGRWGTRDRWIGIPVAWSQICNAKVIVETSFEDFPLDASYGSHFFHNVISM
                     NVGYCSVPNGDSFSRIDWELLDSLPAVNETTFFRHVRFPKPLGVKMDGTQRLIVVTKG
                     "
     gene            complement(3540..5936)
                     /locus_tag="PLW22_03255"
     CDS             complement(3540..5936)
                     /locus_tag="PLW22_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU73753.1"
                     /translation="MKKYLPILLVFIYINHIALRANELSEVCKLNGTVMSNGKALPFA
                     NVIVKGTTIGTVTDEAGSFALCDLKKGTYTITVSAVGFKTAEIKVDIDETDRKTVEIE
                     LQEDLLHLDEVVVSADRGMLKRTQAPVMVNTIAPALFASTQSVVVGEALNFMPGLRLE
                     NDCQNCGFNQVRMNGMEGPYSQILINSRPIFSGLAGVYGLELLPANMIDRVEVVRGGG
                     SVLYGSNAIAGTINIILKDASINSYEVGASGSLIGTGMARSNGPISDYTVNFNNTFVA
                     TNNRTGITIYGFTRDRNMFDANGDGYSELAPMSNVSLGTRFWQRVGERGKISADIFTI
                     REKRDGGNKQDYPLHERDVAEAVKHDMRAGSLIFERYFRQFDLLSIFASAQYLNRDSY
                     YGANQSLSSYGNSFDFTYNTGAQYKAIIGLSTLTAGLELTGSNLNDTKLAYRDLTSPT
                     FDIDPITGDTLGVSFPQVKNTTVSDQSLLTSGIFAQHDMGIGKWKTSVGLRAEQYRIE
                     DFAHPENGIKKGLVLVPRLSIMYNALPWIQTRVSYSQGYRAPQIFDEDLHVETSGARQ
                     VFNRNDPNLKQETSHSFMLSADANREMGNFFIGFLAEIFYTRLQNPFYNEIGAPDENG
                     TVIYTRRNATDGAAVKGINLETKIKSFKDFSLTAGFTLQESLYDTPQEFSTREFFRTP
                     KQYGYLMLDWDFVKDLCLSVSGTYTSPMLAPYFGPNTNPDVGELRSTPDFYDVGVKLT
                     YNFKLEGKTLQLFGGVKNIFNSYQKDFDVGIDRDPSYIYGPTSPRTVYFGIKFGNVLK
                     "
     gene            complement(6064..6411)
                     /locus_tag="PLW22_03260"
     CDS             complement(6064..6411)
                     /locus_tag="PLW22_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73754.1"
                     /translation="MVKSTRRLLISILLVIIALGNILIYSSYSHVHRINGQWVVHSHP
                     YDTQSDKQPVKNHKHTLSEIVLLAQAKQFEESSQNQVYNPEIAVTRIHLKTAVHHLQV
                     ALQLKPNRAPPFV"
     gene            6527..7105
                     /locus_tag="PLW22_03265"
     CDS             6527..7105
                     /locus_tag="PLW22_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOU73755.1"
                     /translation="MEKSIKGTKTEQNLLKAFAGESQAKNRYTFFSKVAKEEGYEQIA
                     ELFMITAMQEEQHAKQFFKFLEGGMVEITASYPAGVIGTTAENLRAAAMGENEEWTSL
                     YPEFAKIAEEEGFPKIATAFKLIAKVEKEHEERYRKLLERVESGTVFEREEEIEWVCR
                     KCGYVHKGKKALKNCPVCNHPQAYFEEKADNY"
     gene            7233..7610
                     /locus_tag="PLW22_03270"
     CDS             7233..7610
                     /locus_tag="PLW22_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU73756.1"
                     /translation="MENSRRYKILIADDDNISVILLTNYLKNIEADFIIARDGEEALT
                     LFEQNTDVDLILMDIKMPKLNGLEVTKQIKSKNPHTKIIAETAFAMVDDEKKAIEVGC
                     DAYITKPINGERLVTMVKSMLGV"
     gene            7939..8736
                     /locus_tag="PLW22_03275"
     CDS             7939..8736
                     /locus_tag="PLW22_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOU73757.1"
                     /translation="MIHDVVLLGAGNLATSLAVALSNAGFPILQVYSRTVESASKLAN
                     LVGAEPICKLESLRTSASIYIVALPDDVIASIVSVLPELKGCVVHTSGSTPMSVFEGL
                     RAKGFGVFYPFQTFSRSKIVPFSNIPICLEASNDECYASLEQFASKLSGTIVPMSSEQ
                     RRWLHLSGVFACNFTNHMLALAYQLTTEHHISFEVVKPLVEETIRKAFEGNPALYQTG
                     PAVRNDIQTMERHAQMLKVFGLADLYKELSLSIQKLSGSDKGLKNRT"
     gene            8752..9282
                     /locus_tag="PLW22_03280"
     CDS             8752..9282
                     /locus_tag="PLW22_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOU73758.1"
                     /translation="MVGKKNFKELLSHVKAFAFDVDGVFTDGTIIIHPSGELLRTSNT
                     RDGYAVHVAIERGFPVAIISGGKSEAVRQRFQGLGVTDIYLGTTDKIEALEDFRFKYG
                     LELSDILYMGDDLPDFEVLQRVGIPTCPRDAAPEIQQISAYISSFAGGKGCVRDVIEQ
                     VLRVNGKWGPSANGLQ"
     gene            9297..10259
                     /locus_tag="PLW22_03285"
     CDS             9297..10259
                     /locus_tag="PLW22_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOU73759.1"
                     /translation="MLKYFRLVRFKNLLIIAATMVLVRYMLIQTILSGYGLELQMPIW
                     YFVALVLATMLVAAGGYVINDYFDTKADLINRPETVVVGKQVTRRGAIAFHLVLSILG
                     IFLGTVVSFRVGRPVFSLLFFLTAGVLWFYSTTYKRQLFVGNLVVAFLTAMVPLIPLI
                     FEFPLLGTFSDVIFIYQLNMKVIVYWVGGYAFFAFMLTLAREIIKDIEDFEGDTSLGR
                     NTIPVHFGVKTSKLIIATVLSLTLIALLVAFSAYLFKLSVKPFDYVSLVYLVIFIVIP
                     IFILIFSVLLASSKKDYHKASSLCKIIMLLGLIYTLIFRFIVNL"
     gene            10272..10880
                     /locus_tag="PLW22_03290"
     CDS             10272..10880
                     /locus_tag="PLW22_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOU73760.1"
                     /translation="MLLHELLQGKRLVLASKSPRRRELLKGLDVEFEIWDTNHDDEEP
                     FLANLPLADVPVYLAKQKATHLADRLDDSTILITCDTIVICCNQILGKPKDEADARRI
                     LSALSNKSHTVITGVCLKSKNSERVFDVATEVHFKRLSMEEIDYYISRYKPYDKAGAY
                     GVQEWIGYVGVERIDGSYFNVMGLPIHTLHSQLISFINSLSK"
     gene            10897..13056
                     /locus_tag="PLW22_03295"
     CDS             10897..13056
                     /locus_tag="PLW22_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOU73761.1"
                     /translation="MRKSFLLILLAVSLTFRGMADEGMWIPMLIGKNIAEMQRMGFKL
                     TAEDIYSVNKASLKDAIVHFGGGCTGELISAEGLLITNHHCGYPEIQSHSTVENNLLA
                     NGFWASSKAEELPNPGLKVRFLVRMDDVTNLVLKNIAPSMTEQERAKSIQQVADSLIA
                     KAEKEGIGYEASIRPFYYGNQYYMFVYQVFEDVRLVGTPPESIGKFGGDTDNWVWPRH
                     TGDFSLFRIYADKNNNPAPYSPSNVPYKPKKFFKISMKGVNEGDFTLVYGFPGRTQQF
                     ITSHAVRLLIDQSNPHKINLRDIRLSIFNKFTESNDTIAIKYAAKHARVANAWKKWIG
                     ETMGLRRLDAVSKKQEQESMFAAWAATNPELKERYGWLLPTFDRLYAELAPLSLVVDY
                     RNEAVYGVEMLSFASRFERLLSMALDPGVDKGKLAEQRNKLVEYVRDFFKDFELSVDR
                     EVFAAMMQAYYERIPQQLQPEFLGKFIQQSGNWRELAKQIYSQTAFADSVRVLTLLND
                     IDSTNAREFLNDPIFSIYFEFEDLYRKNVQKRYFEITDSLDILYRTYVEGLMRMQPDK
                     RFFPDANSTLRIAYGKVGGFAPRDGVVYDYYTTIDGIYEKSQQRDVPDYVPPKRLIEL
                     YQTKDFGPYAVNGTVPVAFIASNHTTGGNSGSPVLDAEGNLIGVNFDRCWESTMSDVM
                     FDPNYCRNISLDIRYALFVIDKYAGAKNLINEMVILQ"
     gene            complement(13125..13832)
                     /locus_tag="PLW22_03300"
     CDS             complement(13125..13832)
                     /locus_tag="PLW22_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOU73762.1"
                     /translation="MANNYDILEQLFDKAKSINPFSDGHFTFGEKYVAVQNGLGQIGV
                     CATLGKKVDHTNLENFDLGKAECRVAANAFANATLNYLVEPDGVGDIFDTVDFSSHTN
                     LVMIGYFGSLVQKLQTKGIKPLVFDIDQHEAPVLPMDKQHEYLTVADCVILTSTSLSN
                     GTFNGIVKTVNPQCSIYMLGPSTPLDDLMFTIPQVKGLFGSIFPIHSSETLNLIAQGY
                     GTKGFMHTMRKVYRIRK"
     gene            13865..14434
                     /locus_tag="PLW22_03305"
     CDS             13865..14434
                     /locus_tag="PLW22_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73763.1"
                     /translation="MEIDNLTERLKYSLRKSEVQQLASFFSAIPNGTDTLFELSLSKN
                     SQLAFHAAWVLENCLLDNTSLLDVHADRLLAVIPSLQNNSVRRHFCKLFKTWLESNQN
                     KLTEWIEARNSKVEGLIEACYGWLLSTDVPVAVRVHCLEIMWILSNQYTWIAEELPNT
                     LRLIQIDSTQGVKAMVRRIKKTRAGNGKA"
     gene            14421..15212
                     /locus_tag="PLW22_03310"
     CDS             14421..15212
                     /locus_tag="PLW22_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOU73764.1"
                     /translation="MVRLDIYLVKKGLCRSRERAKAHITAGEVLVNGKKVTKPSFAVA
                     ETDSIQLSINPNDFFSRGELKLQKAIDDFGLNFQDKLVLDVGASTGGFTYCALKHGAR
                     FVWAVDVGTNQLDPELINNSRVCSLEKTDIRTLDISALGTRVDVITADLSFISLTQVA
                     QSLVKFLKPDGFMVLLIKPQFEAGKENIGKNGVVKDKKVHEVVIENVAKCFAQHNIYL
                     NSITFAPLKGKGYNIEYLALFSNSIKGLPNVKTIVDSAFENNRLV"
     gene            15214..17256
                     /locus_tag="PLW22_03315"
     CDS             15214..17256
                     /locus_tag="PLW22_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOU73765.1"
                     /translation="MTDKTGFDSFVEAILWIVKNLAVGVLILFISRLIFLFSFANWND
                     LSGLSGDIAKAFTVGLKFDLKVLTIALLPCLLLAFVRLIFSDRVGNFTKFFRYYSTAL
                     MILVVMFEVINFYFYKFYHTKISVIIFGFFEDDTWAVLVSIWKEYPVIPILALFIVLI
                     WTFSKLFVHLYKPISCNFCKRLRPIWLRVLIVVAISGLYFLGMLGNIGVTSIDEQHTI
                     ISKNIFVNTLPVNGLFSTKIAWTDRQRSRIDINIPKMLKSLKFKSIEDAAGVFYGLKV
                     DSVSAETFYTTTPQNHFLENNPPHVVFILMESMSNFYIDMHSDSCNMLGSLADVLDSC
                     YLFRNFLPSYSGTIYSLESILVNTPKSPLSQSPYQNFSFDASAAKPFYEKGYTNIFLT
                     GGKMGWRNMDKFIPRQYFNSIEAEPTLRKLYPEGKEGEWGMHDEVLFQRAFDVLANSN
                     GKPYLIFGMTISHHTPYDIPDNYQGYPISIPDWARKRMKFDEAIVLKGLRAYQYANSC
                     LGDFIKRIITSPLGEKTIIVATGDHNIRQNFEYPQADLFMQYSVPLLIYIPPKYRPKN
                     PINTRRFASHKDIFSTLYNLSLSSARYPNFGNNLCNTYCANDFGLYCYSIAADSIGLV
                     DFSSTPLFYRWSDREHRKLEPSNYSPDKHLDSLMLRAKALTACMNYLLIKDIEKNR"
     gene            17363..19768
                     /locus_tag="PLW22_03320"
     CDS             17363..19768
                     /locus_tag="PLW22_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU73766.1"
                     /translation="MLRKTLLLFISIVLLNPLNAQDKGMIKGRVFDSKTNEPIPFATV
                     IVYGKIIGTTSDFDGNFIIAGLEPGWVELQVTAIGFKPYISEAVMVTRSKNIFIDIPL
                     EEMPYEIESVVVKASPFRRSEEAPLSLRRIGIDEIERNPGGNRDISRVIQSLPGVAPS
                     LAYRNDVIVRGGGPNENRFYLDGVEIPNLNHFATQGASGGPVGIINVDFVREVNFYSG
                     AFPASKGNALSSVLDFRQVDGNSERLKVKGAVGASDLALTLDGPLSNNTTMIFSARRS
                     YLQFLFNALGLPFLPNYNDFQFKTKTRIDAKNEITVIGLGAIDEFELNLGLKNPDEYQ
                     RYILGYLPVNTQWNYTLGTVYKHYASNGYHTLVVSRNMLNNRQYKFTNNDDNLPKVLD
                     YTSWEAENKLRYERDLGLKNYSINFGAGLEYARYFNSTYRAKFVGETFTPDTYETNMD
                     LFKWNIFGQVNRTFFTKLTVSLGVRADASSYSAEMNNLFKQLSPRFSASYMLKPNLFL
                     NANVGRYYQLPPYTALGFANTTGSLVNKTNGLSYIQSDHLMAGFEWQPNEQSQITVEG
                     FLKFYDRYPVSINDSVSISSKSADYGTFGDEPLVSTGKGRTFGVELLYRNKKLLGFNT
                     LFSYTLVRSETSLMNSNLKPSGKWIPTSWDNRHLVTLTATRSFKKGWDFGFKWRFVGG
                     PPYTPYDLATSSLINVWNVQRQPSLDYSSFNSLRLQSFHQLDIRVDKSYYFQSWMLNF
                     YVDIQNVYNFQGDRPPLYTTVSDTNGNPVVDPNDATRYLLKKIEGTGGGTVLPTIGVI
                     VEF"
     gene            19781..20506
                     /locus_tag="PLW22_03325"
     CDS             19781..20506
                     /locus_tag="PLW22_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73767.1"
                     /translation="MRTYTILVSVILGLLVLQGCKPTQQSQSETITQELTTSPFSNGV
                     PAQVVVIGGQEYKYPIIAIWVEDLRGNFIGTVYASQSISTGIFRYGVYDKGKWLPGER
                     NRPAALPRWKNLTASNAPRVNQGVDAFSGATPSGSFALKTMLPAGIDEFKVYMEVNKP
                     WDFNNYWHNNRYPGDEEYATSGQPAIIYEALVSRKTGDSLFYFKPIGHSNPSGANGEI
                     IPDIATFTSALKIVERAWIELSK"
     gene            20506..21435
                     /locus_tag="PLW22_03330"
     CDS             20506..21435
                     /locus_tag="PLW22_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOU73768.1"
                     /translation="MEKGIDNKVAVIAGGTGLIGRSLVPMLLNEGYRVKLLTRNPKKV
                     QKSGINADIIEWSGKPDSELTALLEGVDVVVNLSGYNIATLLTKRNRKRIVDSRILPT
                     RALAQAMTSCRKPPKVFVQASAVGVYPCNSSSVLTEKSAVGTGFLPALVSDWENEALA
                     CANVTRVILLRTGIVLASDGGYFPKIAKPIRYFAGAILGDGKNIVPWIHIADHVRAIK
                     FLIDNSAASGAFNLTAPTTNSNADIVFQVAHHYRRPILFRIPSIILHLLPGNMANEVL
                     LANQPVYPERLTQLGFSWNLPVLENAIADLLAK"
     gene            21484..22191
                     /locus_tag="PLW22_03335"
     CDS             21484..22191
                     /locus_tag="PLW22_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU73769.1"
                     /translation="MDNFTVDIRSANIFQEDNLVLSDVNFSLGKGEFVYLVGRVGSGK
                     TSLIKTITAEIPLKEGDGLVCGFNLKKLKRKHIPKLRRKIGVVFQDFQLLTDRSVYDN
                     LLFVLKATGWKDKSAIKQRIAEVLDMVELKFKDYKMPHQLSGGEQQRVVIARALLNNP
                     ELILADEPTGNLDMETSEGIMQILHNLSASGISIIMATHNNQIIKKFPARTIKCEKGK
                     VVETEQMTEIDFESLMD"
     gene            complement(22197..23285)
                     /locus_tag="PLW22_03340"
     CDS             complement(22197..23285)
                     /locus_tag="PLW22_03340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU73770.1"
                     /translation="MLSISKISLRELKLFNDDTGLNEKDFLPISKVRAMSLMANPRAN
                     SNDNVLYQAHWNEKLIAYLTVLPDIAFANNQSIPFAWISGAWVHPSFRRQGIATKLID
                     AVVLDWKGMLMATNFSKITEAVFEKTKTFKTVKKIEGQRFFLRKTLLSSYNCCSHSNP
                     ITYITEKFINLLNHSNLTRKFLKIPKNIEIEYFTRPDDEILNKLTLATANTLTKRSTE
                     EINWIIRYPWLINGLLPDRAAQKFFFASVIPAFAYYMVKVFKDDELIGVLLMQHSNTR
                     FTVPYYWFENGTEDVMAKVILLHASRAKASFINIYNQKIVNSMLNLRPYYFYSKKRTR
                     KYLVADSMADSIGDSFELMDGDGDCAFI"
     gene            complement(23290..24231)
                     /locus_tag="PLW22_03345"
     CDS             complement(23290..24231)
                     /locus_tag="PLW22_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73771.1"
                     /translation="MKKNLEIGFLHREEIDCKRWNATIDNAFNGSIYAYSWYLDVVSP
                     NWGALATNDYSYLFPLPIKRKYGINYIVQPLHCQQLGLFSTNHISADIVDLFIDQIPA
                     KFKYVDLNLNIHNKTNKHTGHINRRKTYMLDLLNYNEQVFRNYNQNTKRNIFKAIANE
                     FTLTTTLNPVDFMKFYRQYGFMVNRKKDVIVIEKLIESLLTHNKATIAGVTDRNNKLC
                     AVALFAESHNTLHYLLGSAEPENKKKGVMFYLMDGVIKNHCGMDKVLDFEGSMILSIA
                     RFFSGFGAKPCTYFNYHRNSLPAGEALVSIKQKLKLI"
     gene            complement(24215..25534)
                     /locus_tag="PLW22_03350"
     CDS             complement(24215..25534)
                     /locus_tag="PLW22_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73772.1"
                     /translation="MELLIYSRQSSTRLEYICRFIFKDILRMPYRLSSSYTEASEYKG
                     PILSYDKQPIPGSISIIPTTLLFETGINQIPMEIGEWNGTPAFPMTWNHKADIPFDLL
                     AGAFYLVTRYEEYLPHKPDKHGRFRFTNSIAYKNGFLEIPVVDVWAHKLGAVIKEKFG
                     NSHSKHRSFTSQITFDLDSAFAFKGKGIIRSALGFTKSAITFDFKKAKTRFRVLANKE
                     QDPFDIYDHLFAKLPAGEATKWFIHVGNWSKYDKQVKTSNPSFLNLVNKLSTRYPIGI
                     HPSYRTFLSDTIFRQELSKLVDILGQAVSASRFHYLRQSIPYSYYPLMRAGITQEYSM
                     GYADKPGFRAGTCTPYPFFDLFDNTTQNLKIFPFAIMDKSLLNIVRYDPNQAFELVKR
                     LVQAVKNVNGNFVSVWHIDYLAQNNPGISLMDILEKTIEMCRNEEKS"
     gene            complement(25594..28071)
                     /locus_tag="PLW22_03355"
     CDS             complement(25594..28071)
                     /locus_tag="PLW22_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOU73773.1"
                     /translation="MIYPDTFEQKVGFDKVRELTLARCLCELGEQRVRDAGFNTNPDI
                     VFEWIEQTHEMKTICQMEDGFPTDGYIDVTPALRKLNVEGQWLDENELQQLRRSLDTI
                     KQLLAFVKRVSDKYPAIAKLATGINYYPYVIERIDSILDRYGRIKDNASAKLAEIRAS
                     IRAKEASVNKLVQGIMRSAREQGVVEADTQPTVREGRVVIPVAAANKRKLKGIVHDES
                     ATGKTVFIEPVEVVELNNEIRELEYEERREIVRILIELADSIRPYLPELLTAYDFMGE
                     IDFIRAKALLAMEFDGVKPILNSNSTVIYLRQARHPILQLSLKREGKRIVPLDIELNL
                     NDRILLISGPNAGGKSVCLKTVGLLQYMLQCGFLPCVLENSEMGIFSKIFIDIGDEQS
                     LENDLSTYSSHLINMKQMLRHADGQSLVLIDEFGAGTEPTAGGAIAEAILQELCNKGT
                     FGVITTHYSNLKHFAANTPGIVNGAMLFDNERIEPLFKLEIGKPGSSFAFEIARKIGL
                     PEKVLDVASQKVGSDYITFEKHLREISRDKRYWEKKRESIRLANKRAEEAEEKLQKEL
                     EELEKKRKEIIATAKKEAQELLANVNKQIENTIRTIKETNADKERTREARKALENLKI
                     EIENPETANALIERKMELIKQRQERKARKNQQKETITSKPVKEDEVRAIEPGDKVKVE
                     GSDMVGEVISISDKSASVAIGGMITVVRVNKLKRLSTGEYREAAKQKPTLNTGFDVYK
                     RRLNFKSDIDVRGYRAEEALEAVQDLIDDALMLGFNRVRILHGKGNGILKQVIRDFLK
                     ATPGVKNFSDEHVEFGGAGITVVDLDV"
     gene            29323..30453
                     /locus_tag="PLW22_03360"
     CDS             29323..30453
                     /locus_tag="PLW22_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOU73774.1"
                     /translation="MDFIISLLISTLVSGVAAAILLSRVYKNSIFVKVGIVWLINLLF
                     IMTTVSIRIKFYDGDTLIRYVMLAVNVLFSVLCFYYATIKVVRPLSRAIDKLYKLAEG
                     DFNVPDDDTNVNPKTDLGQLIQATSMIKDNFGRVVLQIQNNVSRLRDMSDELNTISSQ
                     LAEGAAEESSSIEEISSSMEQIAAHIERNADNSKQTEVYSRKVLDGVKNLNKSAEENL
                     EAINHIAQKINVITDIAFQTNILALNAAVEAARAGAHGKGFAVVAAEVRKLAETSKLA
                     ADEIVQLAGKSVNVAQHTQVMLNELIPDITRTTNLVEEITSASIEQHVGVEQVNNAIQ
                     QQNVVAQQNAAISEEVVTTSNKLSEFANQFVELVSYFKIRKK"
     gene            complement(30738..>31973)
                     /locus_tag="PLW22_03365"
     CDS             complement(30738..>31973)
                     /locus_tag="PLW22_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU73775.1"
                     /translation="ENLRQLLLSPPLGQKSVLAIDPGYRTGCKVVCLDPQGNLLHNET
                     VFPHPPQSEQGKAMSKISALVDTYKIEAIAIGNGTASRETEALIKRIRFNRDVKVFVV
                     SEDGASVYSASPVAREEFPEYDVTVRGAVSIGRRLMDPLAELVKIDPKSIGVGQYQHD
                     VDQTKLKGSLDQVVESCVNHVGVHLNTASKHLLTYVSGLGPQLAKNIVEYRAQNGAFR
                     SRDELKRVPRLGEKAFEQCAGFLRIAEAENPLDNTAVHPESYHIVEKMAADLGCSVVD
                     LIRNEELRAKIKLEKYVTDKVGLPTLNDIMQELSKPGRDPRKAIKVFEFAEGIFSIDD
                     LKPGMVLPGIVTNITNFGVFVDIGVKQDGLVHISQMANRFVSNPLDVVKLHQHVKVKV
                     LEVDIPRKRIQLSMRDVEQ"
BASE COUNT         8533 a         6814 c         7124 g         9502 t
ORIGIN      
        1 aggataaaga taaaagttgg atacgatgaa taacagcaag ccaaatcccg aggagtttta
       61 ccgcctactt gctcaaagca aggaacggtt aaaggagttg aatgccatta atagcgttat
      121 caatattacc aaggaaaaca gaccaattcc tgagaccctc cacagggttt gctttgtttt
      181 acccgatgcc tggcagtatc cggaacacac tgttgcccga ataaaatacg ggcagtatga
      241 gttccaaacc tttggattta aggaaacccc atggtgtcaa cgtcagggtt ttgaaagcat
      301 cgacggtgtc tttggggcaa ttgagatttt ttatactaaa cagttccccg atgagtttga
      361 agggcctttc ctgaaggaag agcgcgacct gcttaataat attgccaaca ttattgaagg
      421 ctacatcaac agtataaagg ggcgcgatgt tttaaaggaa aaaacacagg ctaaacgcaa
      481 gcccgatctc gctgaacccc aaagcaaaag actactgcaa cggtttataa accagcataa
      541 tgccgatagg gatatttacc acgacctgat gccctttaag gttcatgaga tattgcttat
      601 ctccaccctt tacgatgcat acagcattga gcgcgaggat aggctaaccg ataacatact
      661 tggcgaatat tccaagttga gcctctcaag cgtgccccgt attacagggg tatcaacact
      721 cgacgaagcc ctggagaagc tcgaggagcg atactttaac cttatcatta tcatgatggg
      781 ggccgatacg gttaccccct tacgcatggc ttcacgtatt aagggcgagt acaagcacat
      841 tcccctttac ctgcttgtta acaacagcat tattgttaat gagattgaga aaaaccccaa
      901 caccattgag ggggtcgata ggatttttgt atggaatggc gagcccaaga tattcttctc
      961 aatgataaag ctgcttgagg atcgcgccaa tattgaaaac gatacccgaa tagccttaac
     1021 gcgcgttatt ttgctggttg aggattcccc aaaatactac tcgcgatact taccactact
     1081 gtatggctcg gtgttagacc aaaccaagcg ggttattgag gacgtaagca ccgatgacct
     1141 ttacaaggtg ctgcgtatcc gaattcgtcc aaaaattctt ttggctggca catacgagga
     1201 ggccattgac cttttcaccc gttacaagga ttacatgctt tgcatgattt ccgatgtaaa
     1261 gtttttccgc gatggaagac tcgacgaaaa tgctggtgtc cggttggttg agcatgcccg
     1321 taagatgctc cccgacttac caataatcct tcaatcgttc gaaaaatcga acgaagccat
     1381 tgcccataag ctaaaggttg cttttttgga caaaaactcg gagagtttgc tcaacgatat
     1441 taaaagtttt ttgagcaact atcttggttt tggcgatttt gtttttaagg acgaggctgg
     1501 gaatcccatt gccattgcaa caaccatgga ggagtttgag cgcgcgctgc gtatcatacc
     1561 cgatgagagc ttactttacc atgcccaaaa aaaccacttt tccatgtggc tctcggcacg
     1621 aggtgagata caggtggccc gtatcattca cccgtcaaag attgacgatt tttccggccc
     1681 ggccgagatc cgggagtacc tgcttagcac cctgaaaaaa taccgccagg agaagcgaag
     1741 gggcaagatt gtaggttttg ataccaactg ggaggttgat tccagcaata ttgttagcct
     1801 tgccgatggt tcggttgggg gcaagggtcg agggcttgct tttatcaata ccctgctcta
     1861 caccttcgat atttcgcagt atacgcccaa tatcaacctg cttacacccc gaacttcaat
     1921 aattggcacc aacgagtttg agtgcttcat ggcacgcaat gggctatacg ataaggtgtt
     1981 tgcttgcaaa agctttgagg agctgcaaag gcattttata aaggccgagc tttcggatca
     2041 gctaaagctt aggctcgaca ggctcttgca gatttactac aggccgctgg ctgtgcgttc
     2101 atcgggtatg cttgaggatt caatcatgca gccctttgcc ggggtatttg aaacctacct
     2161 tatcccaaat gctcatcccg acaggagcgt caggctacaa cgcctgatga acgccattaa
     2221 gctggtttac gcatcggtat tttcaccaac ggcaagggct tacattaaag ccatgaacct
     2281 tagggttgag gatgaaaaaa tggccgttat cattcaggag gttgtgggca ataagtttga
     2341 gaactactac tatccacaca tcagtggggt agcccaatcg cataactact acccgtttgg
     2401 gcatatcaac cccgacgatg gatttgccaa cattgccata ggtttgggaa aacacgtggt
     2461 ggagggaggt cgctcatacc ggttttgccc aaaataccca acgttggtca actataccat
     2521 ggaggacctt attaaaaatt cacaggtcga ttttctggca gttgacctca gcaagtgcga
     2581 ttacgacttg ctaaccggcg atgaagcagg acttgcccgc ctcgacctct acgaggccga
     2641 aaggcatgga accctcaagc attgtgcatc ggtttacagc tccgagaact cctcgctcat
     2701 tccgggtata gggcaatcgg ggccaagagt ggtaaacttt gccaatatcc taaagtataa
     2761 ctacgtaccc cttgcccaaa ccatcgatgt gattctcgat attgttgagg aggctctcgg
     2821 cgctccttgc gaaattgagt ttgctgttga ccttaaccgc gacgctaact ttaggtcatc
     2881 gttcttcctg cttcagatta agccgctaat ggcaagcgct caggaatata ggctcgatat
     2941 taacactatt gatcgggctg aagccatttt gctctccaat aatgcaatgg ggaatggaca
     3001 cctaaccaac attgaggatg tagtatatat tcgccgcgag gcattcaata aggcaatgac
     3061 tttggaaatg gctaacgagg tggaatggct caacaataca cttgaggacg aagggcgaaa
     3121 gtatatcctt atagggccag gtcgatgggg aactcgcgac aggtggatag gtattccggt
     3181 tgcctggtct caaatttgca atgccaaggt tattgtggag accagctttg aggatttccc
     3241 tctcgatgcc tcttacggct cacacttctt tcacaacgtt atctcaatga atgtgggtta
     3301 ctgctctgtt cccaatggcg actcattctc acgaatcgat tgggaactgc tcgatagcct
     3361 acctgcagtt aatgaaacaa catttttccg gcatgtacgc ttcccaaaac ctttgggtgt
     3421 taaaatggat ggcacacaac ggttgattgt ggtgactaaa ggatagtaat agccgttttg
     3481 ctcggtgaag cagtttggca aaaaacaaaa tccccctctg agggggattt tgtttgtgtt
     3541 tacttcagta catttccaaa ctttattcca aagtaaactg ttcgtggcga tgtggggccg
     3601 taaatgtacg atggatccct atcaataccc acatcaaaat ccttttggta ggagttgaaa
     3661 atattcttta ccccaccgaa tagctgaagc gttttaccct caagcttgaa gttataggtt
     3721 agcttaaccc ctacatcata aaagtcagga gttgagcgga gttcccctac atcgggatta
     3781 gtgtttggcc caaagtaggg ggcaagcatt gggcttgtat atgtgcccga aaccgataga
     3841 cataaatcct ttacaaagtc ccagtcgagc attaggtagc catattgctt gggagtacga
     3901 aagaactcac gcgtgctaaa ctcctggggg gtatcgtaaa gcgattcctg tagggtaaaa
     3961 cccgcggtaa gtgaaaagtc cttaaacgac ttgattttgg tttcaaggtt tatcccctta
     4021 accgctgcac catcggtggc attgcgacgg gtgtaaatta ccgttccgtt ttcgtcgggt
     4081 gcgcctatct cgttatagaa tggattttgt aggcgtgtat aaaagatctc agccaaaaag
     4141 ccgataaaaa agttccccat ttcccggttt gcgtcagctg agagcataaa gctgtggctg
     4201 gtttcctgct ttaggttagg atcgttcctg ttgaatacct gacgggcacc ggaggtttcc
     4261 acatgcaagt cctcatcaaa gatctgtgga gcacggtagc cctgtgagta cgatactctt
     4321 gtctgtatcc acggaagcgc attgtacatt atgctcagcc gtggcacaag caccaatcct
     4381 ttttttattc cattttccgg atgggcaaaa tcttcaatac ggtactgttc agccctcaag
     4441 ccaaccgaag ttttccattt cccaatgccc atgtcgtgct gggcaaaaat tcccgatgtc
     4501 aaaagtgatt ggtccgaaac ggttgtgttt ttaacctggg ggaaacttac tcctaacgta
     4561 tcgcccgtta ttggatcaat atcaaaagtg ggactggtta aatcgcggta agccagcttg
     4621 gtgtcgttaa ggttgctgcc ggtaagttca agtccagcgg tcagggtcga tagcccaata
     4681 atggccttgt actgtgcccc ggtgttatag gtaaaatcga atgagttgcc gtaactgctc
     4741 agcgactgat ttgccccata gtatgagtcg cggttcaggt actgtgctga ggcaaagatc
     4801 gagagtaagt cgaattgcct gaagtaacgc tcaaagataa gcgaacctgc acgcatgtcg
     4861 tgcttaacag cttcggctac atcgcgttcg tgcaacgggt aatcctgttt gtttccacca
     4921 tcgcgtttct cccgaatagt gaaaatgtcg gcgctaattt tacctctctc gccaacgcgt
     4981 tgccagaatc gggtgccaag gcttacgttg ctcatgggtg caagttccga gtaaccatcg
     5041 ccattggcat cgaacatgtt cctgtcgcga gtaaacccgt aaatggttat gcctgtacgg
     5101 ttgtttgtag caacaaatgt gttgttaaag ttaacggtat agtcgctgat aggcccattt
     5161 gaacgtgcca tcccagttcc aatcagcgaa cccgatgccc caacctcata ggagtttatg
     5221 ctggcatctt ttaaaatgat gtttattgtt ccggctatag cattgctacc gtaaagcacc
     5281 gatccaccac cacgcacaac ctcaacacgg tcaatcatgt ttgcagggag cagttccaga
     5341 ccgtaaacgc ctgccagccc gctaaaaatt ggccgactat tgatgagtat ttggctgtag
     5401 gggccctcca tgccattcat gcgaacctgg ttgaatccgc agttctggca atcgttctca
     5461 agccttaggc ctggcataaa gtttagggct tcaccaacca caaccgattg tgttgaagcg
     5521 aaaagtgcag gtgcaatggt gtttaccatt actggggctt gggtacgctt aagcataccc
     5581 ctatcggccg aaaccacaac ctcatcaagg tgtaagaggt cctcctgcaa ttcaatttcc
     5641 acggttttcc tgtcggtttc atctatatca accttgattt cggcagtttt aaatcccact
     5701 gcgctaaccg taatggtata agttcccttt ttcaggtcgc aaagtgcaaa gctgcccgct
     5761 tcgtcggtta cagtccctat tgtagtgcct ttaacaatga catttgcaaa gggtagggct
     5821 ttaccattgc tcataactgt tccgtttaac ttgcaaactt ccgatagttc atttgccctt
     5881 agtgcgatat gattgatgta tatgaatacc aatagtattg gtaaatactt tttcatgctg
     5941 aaaaaaaatt aacgaagtta atgctgattt gatttctata accatgcaag ccgatgcatt
     6001 gcaagcatca gtataggaac ccgaatggtt tcaggtttac tgatggtttt acattgtggt
     6061 tatttataca aacgggggtg ccctattagg cttaagctgt aatgctactt gaaggtggtg
     6121 tactgctgtt tttagatgta ttcgggtaac agcaatttcg ggattataaa cctggttttg
     6181 tgagctttcc tcgaattgtt ttgcctgggc aagcaataca atttccgata aggtatgctt
     6241 atggttttta acgggctgct tatcggattg tgtatcgtat gggtgtgagt ggactaccca
     6301 ttgtccgtta attcggtgaa cgtggctgta gctgctgtaa ataagaatat tccccagcgc
     6361 aattattacc aggagtatcg atataagcag tcttctagta cttttcacca tctcggctgt
     6421 tagcgaatta acactgcaaa ggtaacttta gaattattct aaacaatggt ttattggtgt
     6481 tttttgcgta ttttagcaaa actataaaca taaaccagta atagcaatgg aaaagagcat
     6541 aaagggaaca aaaaccgaac agaacctact aaaggctttt gccggtgaat cgcaagcaaa
     6601 gaaccggtac acctttttct caaaggttgc caaggaggaa ggctatgaac aaatagccga
     6661 gttgttcatg ataaccgcca tgcaggaaga gcaacatgcc aaacagtttt tcaaattcct
     6721 tgagggtggc atggttgaaa taactgcaag ttatcctgca ggagttattg gcactactgc
     6781 cgagaactta agggctgccg ccatgggtga aaacgaagag tggacaagcc tataccctga
     6841 atttgctaag atagccgagg aagagggttt tccaaagatc gcaacggctt ttaaattgat
     6901 tgcaaaggtt gaaaaagagc atgaggagcg ttaccgcaaa ctgcttgaac gggttgagag
     6961 cggaactgtt tttgagcgcg aggaggaaat tgaatgggtt tgtcgtaaat gcggttatgt
     7021 tcataaaggg aaaaaggctc taaagaattg ccctgtttgt aaccatcctc aggcatactt
     7081 tgaggaaaaa gctgataatt actagtatgc cattaattat ttagccgcag ccttgctgcg
     7141 gtttttttat gtttaagaac aattagttgt gtattaattt aaagcgtttt aactttgcgc
     7201 tttagaatga acgtctgagt aaaattaggg ggatggaaaa ctccagaagg tataagatac
     7261 tgatagctga cgacgataat atttcggtaa tactgcttac caattaccta aaaaacattg
     7321 aagcggattt cattattgct cgcgatggcg aggaagcact tacccttttt gagcaaaaca
     7381 ccgatgttga ccttatcctt atggatataa aaatgcccaa gctgaacggg ttagaggtca
     7441 caaagcagat aaaatccaaa aatcctcaca caaaaattat tgccgaaaca gcctttgcca
     7501 tggttgacga tgagaaaaag gccattgagg taggctgtga tgcctacatc actaagccaa
     7561 taaatggcga aaggttggtg actatggtaa agagcatgct gggagtttaa ggttagctgg
     7621 taattgacag ttgttggttg atagttggta gttgatagtt gatagttgtt ggttgatagt
     7681 tgttggttga tagttgttgg ttgttggttg ttggtttaag agtgtccatt cccaatcatt
     7741 ccgaatcatt ccgagttttc cgagaattcc gactcattcc tccccatcct cgttgggtat
     7801 acttttcact tttcactttt cctctaactt cctctaactt cttctaactc cctcatattc
     7861 tcaattcttc ttttcacgtt tcacggactt cttttcattt ttctttctta cctttatacc
     7921 tcaaattaca tctacgccat gatacacgat gtggtactgc ttggtgcggg caatttggca
     7981 acctcgttgg ctgttgcgtt aagcaatgca gggtttccta tcctacaggt ttactccaga
     8041 accgtggaga gtgcaagtaa attggcaaac cttgttgggg ctgagccaat ttgcaaactt
     8101 gaaagtttga gaaccagtgc gagtatctat attgttgctc ttcccgatga tgttattgcc
     8161 agtattgttt cagttttgcc tgagctaaag ggttgtgttg ttcatactag tggaagtact
     8221 cccatgtcgg tttttgaagg gttgagggct aagggctttg gcgtttttta cccttttcaa
     8281 accttttcgc ggagtaagat agttcccttt agtaacattc ctatttgcct tgaggcaagt
     8341 aacgatgaat gctacgcttc cctggagcag tttgcctcaa aattgtcggg aactatagtt
     8401 cccatgagtt cagagcagcg aaggtggtta cacctttcag gtgtttttgc atgtaatttt
     8461 acaaaccaca tgttggcttt ggcataccaa ttaaccacgg agcatcatat tagttttgag
     8521 gttgttaaac ctcttgtgga ggaaaccatt cgcaaggctt ttgaggggaa tccggcactt
     8581 taccaaaccg gtcccgctgt acgtaacgat attcaaacca tggagcgcca tgctcagatg
     8641 ttgaaagtat ttggcttggc cgatttgtac aaagagctat ctttgagcat tcaaaaattg
     8701 tcaggaagtg acaaagggtt aaaaaaccgt acataggcat aatctttctt tatggtgggt
     8761 aaaaagaatt ttaaggaatt acttagtcat gtcaaggcat ttgcctttga tgttgatggg
     8821 gtgtttaccg atggtacaat tatcattcac ccatcgggtg aactacttag aacctcgaac
     8881 acgcgcgatg gctatgctgt tcacgttgct atagaacgtg gttttccggt ggctatcatt
     8941 tccggaggca agtccgaggc ggttcgccaa cgctttcagg gattaggtgt aaccgatatc
     9001 taccttggta ctaccgataa aattgaggct cttgaggatt ttcggtttaa gtatgggctt
     9061 gaactctccg atatacttta catgggcgac gatttacccg attttgaggt tctgcaacga
     9121 gtaggaatcc caacctgccc aagggatgca gcacctgaaa tacagcaaat ttcagcctac
     9181 atttcgagtt ttgcaggagg caagggctgt gtgcgcgatg taattgagca ggtgttaagg
     9241 gtcaatggga aatggggccc atccgcaaac gggttacagt aaaaaacatt tgtgctatgc
     9301 ttaaatactt tcgattagtc cggttcaaga acttgctgat tattgcagca acaatggtgc
     9361 tggttaggta tatgcttata caaaccatac tttcaggtta tggattagaa ttacagatgc
     9421 ccatttggta ttttgtagcc ttagttcttg caaccatgct ggttgcagcc ggcggatacg
     9481 ttataaacga ttactttgat actaaagccg atctgattaa tcgtcccgaa acggtggttg
     9541 ttggaaaaca ggtaacccgt cggggagcaa ttgctttcca tttagtgctt tccattctcg
     9601 ggatttttct gggcacggta gtttcgtttc gtgtgggaag accagtgttt tcgttactat
     9661 tcttccttac tgccggtgtg ctttggtttt actcaactac ctataagcgt cagctatttg
     9721 tgggtaacct ggtggttgcg tttttaactg caatggttcc gctcatcccc ctgatttttg
     9781 agttcccctt gcttggaacc ttcagcgatg tcattttcat ttaccagctt aacatgaagg
     9841 tgattgttta ctgggtaggg gggtatgcct tttttgcctt tatgcttact cttgcacggg
     9901 aaattattaa ggatattgaa gattttgaag gcgatacatc gcttgggcga aacacaatac
     9961 ccgttcactt tggggtcaaa acttcaaaat tgattattgc tacagtgttg tcgttaaccc
    10021 tgattgccct gttagttgcc ttttcggcat atctttttaa gctttcggtt aagcctttcg
    10081 attatgtttc gttagtttac ctggtgatat tcatagtaat ccccattttt attttgattt
    10141 ttagcgtatt gcttgcatcg tccaagaagg attaccataa ggcaagttcg ctatgcaaga
    10201 ttatcatgct tttagggctg atttacacac tcattttccg ttttatagta aacctgtaaa
    10261 gttttttgtt gatgttgcta catgaattgc tccaggggaa acgactagtt ctggcatcta
    10321 aatcgcctag gaggcgtgag ctgcttaaag ggcttgatgt ggaatttgag atatgggata
    10381 caaatcacga cgatgaggag ccattccttg ccaaccttcc tttggccgat gtgccggttt
    10441 acctcgcaaa gcaaaaggct acacacttag ccgataggct cgatgatagc acaatcctta
    10501 tcacctgcga tacaattgtt atttgctgca accaaatcct tggcaagcca aaggatgaag
    10561 ccgatgcccg ccgaatacta agcgctttgt ccaataagtc gcatacggtt attaccggcg
    10621 tgtgtcttaa atccaaaaat tccgaaaggg ttttcgatgt tgcaaccgag gttcatttca
    10681 aacgcttaag tatggaggag atagattact acatcagcag gtataaaccg tacgataagg
    10741 ctggagctta cggggtgcag gagtggattg gatacgtggg ggttgaacgc atcgatggtt
    10801 catactttaa cgtaatgggg ttacccattc atacgctgca ctcgcaatta atctctttta
    10861 tcaattcact gtcaaagtag aaaaatccga atagctatga gaaaatcatt tttgttaata
    10921 ctgcttgctg tgtcccttac tttcagggga atggccgatg agggcatgtg gataccaatg
    10981 cttattggta aaaatattgc cgagatgcaa cgaatgggct ttaagcttac tgccgaggat
    11041 atttatagcg taaataaggc aagccttaag gatgccatag ttcattttgg cgggggctgc
    11101 accggtgaac ttatctcggc tgagggcctt ctgattacaa accatcattg cggataccct
    11161 gaaatacaat cgcactcaac ggttgagaac aaccttttag ccaacggctt ttgggctagc
    11221 agtaaagccg aagaattgcc caatccgggt ctcaaggtga ggttccttgt gcgcatggac
    11281 gatgtaacca atttagtcct gaaaaacata gccccatcta tgaccgagca ggaaagggca
    11341 aagagcattc agcaggtggc cgatagccta attgcaaaag ctgaaaagga aggcattggt
    11401 tacgaagcta gcattcgtcc tttttactat ggcaatcagt attacatgtt tgtttaccag
    11461 gtgtttgaag atgtgcgcct ggtgggtaca cccccagagt caataggtaa gtttggtggc
    11521 gataccgaca actgggtttg gcctcgccat actggcgatt tctcgctatt ccgtatctac
    11581 gccgataaga acaataatcc agcaccatac tcaccaagca atgttcccta caaacccaaa
    11641 aaattcttca aaatatcgat gaaaggtgtt aacgaaggcg attttaccct tgtctatggc
    11701 tttcccggcc gcactcagca gtttataacc tcccatgccg ttaggttgct tattgaccaa
    11761 agcaacccgc ataaaataaa tctgcgcgat atccgccttt caattttcaa taagtttacc
    11821 gagtcaaacg atactattgc cattaagtat gcggctaagc atgcccgagt ggccaatgcc
    11881 tggaaaaagt ggattggtga aacaatgggt ttgcgcaggt tggatgctgt ttccaaaaag
    11941 caggagcagg agagtatgtt tgctgcgtgg gcggctacaa accctgagct taaggagcgt
    12001 tacggttggt tacttcctac cttcgatagg ctttacgctg agttagctcc tctgtcgctg
    12061 gttgtggatt accgcaacga ggcggtttat ggggttgaaa tgctcagctt tgcatcgcgt
    12121 tttgagcgat tgttgagcat ggctcttgac cccggtgttg ataaggggaa attggctgaa
    12181 cagcgtaata agctagtgga gtatgttcgc gatttcttca aggactttga actcagtgtt
    12241 gacagggagg tttttgctgc tatgatgcaa gcctactacg agcgtattcc ccaacagctt
    12301 caacctgaat ttttaggtaa gttcatccag cagtcgggta attggcggga gttagctaag
    12361 caaatctatt cacaaaccgc ttttgccgat agcgtaaggg ttttaaccct tttgaatgac
    12421 attgatagca ccaatgcccg tgagttcctg aacgacccaa tctttagcat ctactttgag
    12481 ttcgaagacc tttaccgtaa gaatgtgcaa aaacgctact ttgaaattac cgatagtctg
    12541 gatatccttt acagaaccta cgtggaaggg ttaatgcgta tgcaacccga taagcgattc
    12601 tttcccgatg ccaactctac cctccgcatt gcatacggca aggtgggcgg ttttgccccg
    12661 cgcgatggtg tggtttacga ctactatacc actatcgatg ggatttacga aaaatcgcag
    12721 cagcgcgacg ttcccgatta cgttccgcca aaacggttga tagagcttta ccagacaaag
    12781 gattttggcc cctatgctgt taatggaact gttccggttg cattcatcgc ctcaaaccat
    12841 acaactggcg gtaattccgg tagtcccgtg ctcgatgccg aaggcaatct tataggtgta
    12901 aactttgacc gctgctggga aagcaccatg agcgacgtaa tgtttgaccc caactactgc
    12961 cgtaacatat cgctcgatat acgctacgcg ctttttgtta tcgataagta tgccggtgct
    13021 aagaatttga ttaacgagat ggtgattttg caataatagg taaatatcaa gtattaactt
    13081 aaacgcttag cttctttatg tagctaagcg ttttttcgta atagctattt tcttatcctg
    13141 taaaccttcc gcatggtgtg cataaagccc ttggtaccgt aaccctgggc tataaggttg
    13201 agggtttcgg agctatggat cgggaaaatt gagccaaata gtcccttcac ctgtggaatg
    13261 gtaaacatca ggtcgtccag cggggtggag gggccaagca tgtatatgga gcattgcggg
    13321 tttacggttt ttacaatccc gttgaatgtt ccattgctta ggctggttga ggtgagaata
    13381 acacagtcgg caaccgtgag gtattcgtgc tgtttatcca tcggtagcac tggcgcttcg
    13441 tgctggtcga tgtcgaaaac caggggtttg atacccttgg tttgcagctt ctgaaccagt
    13501 gagccaaagt agcctatcat caccaggttg gtatggctgc taaaatcaac cgtgtcaaaa
    13561 atatcaccaa ccccgtcggg ttctacaagg tagtttaggg ttgcgttggc aaatgcgtta
    13621 gcagcaaccc ggcattcagc tttgccaagg tcaaaatttt caaggtttgt gtggtctacc
    13681 tttttaccta atgtggcgca tacacctatt tgtcccagtc cgttctgaac tgccacatac
    13741 ttttcaccaa aggtaaaatg cccatcgcta aaggggttta tgctctttgc cttatcaaaa
    13801 agctgttcga gtatatcgta attgttggcc atacactaaa tttgtggttt cgcaattttt
    13861 gctgatggaa atcgataacc taacggaacg gctaaaatac tcacttcgta aaagcgaagt
    13921 gcaacagctg gcatcttttt tttctgccat acccaatgga actgatacgc tttttgaact
    13981 aagcttgtcc aaaaatagcc agttggcttt ccatgcagct tgggttctgg agaactgctt
    14041 attggataat acctcgcttt tagatgtcca tgccgatagg ttactggctg ttattcccag
    14101 tttgcaaaac aactcagttc gtcgccattt ctgcaagctg ttcaaaacat ggttggaaag
    14161 taatcaaaat aaacttactg aatggattga agcgcgcaat agcaaggttg agggattgat
    14221 agaggcttgc tatggctggt tgcttagcac cgatgttccc gtagcagtta gggttcattg
    14281 ccttgaaatt atgtggattc tatcaaatca atatacctgg attgccgagg agttgcctaa
    14341 taccctaagg cttattcaaa tcgattcaac tcagggggtt aaagctatgg ttcggcgaat
    14401 aaaaaagaca agggcaggga atggtaaggc ttgacatata tttggtgaag aaaggattat
    14461 gccgctcgcg cgagcgcgca aaagcgcata ttaccgcagg cgaggttctg gtgaacggga
    14521 aaaaggttac taaaccatcg tttgcggtag ccgaaaccga ctctattcag ctctccatta
    14581 accccaacga tttttttagc cgcggggaac ttaagcttca aaaggcaata gacgattttg
    14641 ggctcaactt ccaagacaaa ctggtgctcg atgtgggggc ttcaaccggt ggctttacct
    14701 attgcgccct aaagcatggt gcaaggtttg tttgggctgt tgatgtggga accaaccagt
    14761 tagacccaga attgattaac aattcacggg tctgctcctt ggaaaaaact gacattcgaa
    14821 ccttggatat ctcggcattg ggtactaggg ttgatgtaat taccgccgac ctttcgttca
    14881 tttcgcttac gcaggtagct caatcgttag tgaaattcct taaacccgat gggtttatgg
    14941 ttttgctcat taagccacag tttgaagcgg gaaaggaaaa tattggtaaa aatggggtgg
    15001 taaaggataa aaaggttcac gaggttgtaa ttgagaacgt ggcaaagtgc tttgctcagc
    15061 ataacatcta cctgaatagt attacctttg cacccctcaa gggtaagggc tataatattg
    15121 aataccttgc cctttttagc aattccatta aaggtttacc caacgtaaag acaatagttg
    15181 atagcgcttt tgaaaataac aggctagttt agtatgacag ataaaacggg tttcgatagc
    15241 tttgttgagg ctattctttg gatagtaaaa aacttagcgg ttggagtact aatccttttt
    15301 atttcgaggt taatatttct gttttcgttt gctaattgga acgaccttag tggattgtcg
    15361 ggcgatattg caaaggcttt caccgtgggc ttaaagttcg acctaaaagt gctgactatt
    15421 gccctgttac catgtttatt actggctttt gttagattga tttttagcga tagggtgggg
    15481 aactttacaa agtttttcag gtattactct actgcactta tgattttggt ggtgatgttt
    15541 gaggtaatca acttttactt ttataagttc taccatacaa agattagcgt aataattttt
    15601 ggtttttttg aggatgatac ctgggctgtg ctagtttcca tttggaaaga atatcccgta
    15661 atcccgattc tggcgttgtt tatagtgttg atttggactt ttagtaagct gttcgtgcat
    15721 ctttataagc ccataagttg caacttctgc aagaggttaa ggcctatttg gctgcgagta
    15781 ttaattgtgg ttgcaatttc gggcttatac tttttgggaa tgcttggcaa cataggcgtt
    15841 acttcgattg atgagcagca tacaatcata tctaaaaata tttttgtaaa tacgttgcct
    15901 gttaacggtc ttttcagcac aaaaattgcg tggactgata ggcaaaggag ccggattgac
    15961 atcaatatac ctaaaatgct caagtcctta aaattcaaat caatagagga tgcagcaggt
    16021 gttttttatg gtttaaaggt cgatagtgta tcagctgaaa ccttttacac caccactcct
    16081 caaaaccatt ttcttgaaaa taatccgccg catgtggttt ttatcctaat ggaaagcatg
    16141 agtaactttt atatcgatat gcattccgat agctgcaaca tgttgggttc ccttgccgat
    16201 gttttggata gctgctacct gttccgtaac tttttaccat cgtactcagg aacaatctac
    16261 tccttagaaa gtatacttgt aaacactccc aaaagtccac tatctcagtc accttatcag
    16321 aacttttcat ttgatgcttc ggcagcaaag cctttttatg aaaagggata tacaaatata
    16381 tttttaaccg gaggtaaaat gggttggagg aatatggata agtttatacc acgtcagtat
    16441 tttaatagca ttgaagcaga accaaccctc aggaagttat accccgaggg caaggaaggg
    16501 gagtggggca tgcacgatga ggtgcttttc caaagggctt tcgatgtgct tgcaaattct
    16561 aatggcaaac cttacctgat attcggtatg accatttcgc accatactcc atacgacatt
    16621 cctgacaact accagggtta ccccatttca attccagatt gggcgcgcaa acgcatgaag
    16681 ttcgacgagg caatagtgtt gaaaggtctt agggcatacc agtacgccaa tagctgtttg
    16741 ggcgatttta tcaaacgtat tattacttcc ccccttggcg aaaaaactat aattgtagcc
    16801 actggcgacc acaatatccg acagaacttt gagtatccgc aggccgattt gtttatgcag
    16861 tactcagttc cattactcat ctatattcca cctaaatatc gtcctaaaaa tccaatcaat
    16921 accaggcgtt ttgcatcgca taaggacatt ttttctaccc tttacaacct ttcgctttca
    16981 agtgcgcgat atccaaactt tggaaacaac ctatgtaaca cctattgtgc aaacgatttt
    17041 ggactttact gctacagcat tgctgccgat agcattggtc tggtcgattt tagctcaacc
    17101 cctttgtttt atcggtggag cgatagggaa catcgtaagc ttgagccaag caactattca
    17161 cccgataaac atctcgactc acttatgttg agggctaagg cgcttaccgc atgtatgaac
    17221 tacctgctta ttaaagacat tgagaaaaac aggtaggttt gtttagggga attgaaaatt
    17281 tagctatttt tatataagac aatatcgcaa atgttaaaca aaacatgctg cgatgcgttt
    17341 cttatgcaaa attctttatg ctatgttacg taaaacatta ttgcttttta tatcgattgt
    17401 actattaaac ccactcaatg cccaggataa gggtatgata aaggggagag tatttgatag
    17461 taaaaccaac gaaccaattc catttgccac agtaattgta tatggaaaaa ttattggcac
    17521 cacctctgac tttgatggca attttattat tgccggtctg gagccggggt gggttgagct
    17581 acaggttact gccattggtt ttaaacccta catttccgaa gcagtaatgg taactcgctc
    17641 aaaaaatatt tttatcgata ttccactgga ggaaatgccc tatgagattg agagtgtggt
    17701 ggtaaaggca tcgcctttta ggcgaagcga ggaagccccg ctttctttac gccgtatcgg
    17761 tatcgatgag attgagcgta acccgggagg aaaccgcgac attagccgcg ttattcagtc
    17821 gttgcctggc gttgctccct cgcttgctta ccgtaacgat gttattgtta ggggtggagg
    17881 gccaaatgag aacaggttct acctcgatgg tgttgaaatc ccaaacctga atcactttgc
    17941 cacacagggg gcttccggag gcccagtagg catcattaat gttgattttg tgcgtgaggt
    18001 aaacttctat tctggcgctt tcccagcctc aaagggcaac gcgcttagtt cagtactcga
    18061 tttccgtcag gttgatggaa acagtgagcg actaaaggtt aagggagcag taggagcatc
    18121 ggatttagcg ctgaccctcg atgggccatt aagcaataat actaccatga ttttctcggc
    18181 tcgccgctca tacctgcaat tcctttttaa cgccttagga cttccgtttt tgcccaacta
    18241 taacgacttt cagttcaaga ctaaaacccg tatcgatgca aaaaatgaga ttaccgtaat
    18301 tggactcggt gccattgatg agtttgagct gaacctgggc ttgaaaaacc ccgatgagta
    18361 tcaacgttac atccttggtt atttacctgt aaatacacaa tggaattata cccttggaac
    18421 agtttacaag cactatgcca gcaatggtta ccataccctt gtggttagcc gaaatatgct
    18481 aaacaacagg cagtacaagt ttacgaataa cgatgataac ctccccaagg ttctcgacta
    18541 tacatcgtgg gaggccgaga ataagttacg ctatgagcgc gatttagggt tgaaaaatta
    18601 cagtataaat tttggagcgg gtttggagta tgcccgatat tttaactcca cttatagagc
    18661 aaagtttgtt ggtgaaacct ttacccccga tacttatgaa accaacatgg atttgtttaa
    18721 gtggaatatt tttggccagg tgaacagaac ctttttcaca aagttaacgg tttcgttagg
    18781 ggttcgggcc gatgcaagta gctattcagc cgagatgaat aacctcttta agcagcttag
    18841 ccctaggttt tcagcgtcgt atatgcttaa acccaattta tttttgaatg caaatgttgg
    18901 aagatactac caactgcctc catacacagc cttggggttt gccaacacca ctggctcttt
    18961 agtgaataaa acaaatggtt tgtcgtatat ccaatcggac catctgatgg ctggttttga
    19021 atggcaaccc aatgagcaaa gtcagattac tgttgaaggc ttcctgaaat tctacgatag
    19081 gtacccggtt tccataaacg actcggtgtc aatatcaagc aaaagtgccg actatggaac
    19141 ctttggcgat gagcctttag tgtccacagg gaaagggcga acatttgggg ttgagttact
    19201 ttaccgaaat aaaaagcttt tgggtttcaa tactcttttc tcatacacct tggttagaag
    19261 cgaaactagc ttaatgaata gtaacctaaa accttcaggt aaatggattc caacatcctg
    19321 ggataatcgc catttggtta cacttacagc cactcgttcc tttaaaaaag gatgggattt
    19381 tggcttcaag tggcgatttg taggtggccc gccgtacacc ccttacgatt tagcaacctc
    19441 atcgttgata aatgtatgga atgttcagcg tcagccatcg ctcgattact ccagtttcaa
    19501 cagtcttcga ctgcagtcgt tccaccagct cgatatccgt gtcgataagt cttactactt
    19561 tcagtcgtgg atgctgaact tctatgtcga tattcagaat gtttacaact tccagggcga
    19621 taggcccccg ctttacacta cagtttccga taccaatggt aatcctgtgg ttgacccaaa
    19681 cgacgctacc cgatacctgc taaagaagat tgagggaacc ggtgggggaa ccgtattacc
    19741 taccattggc gtaattgttg aattctaaaa aatgataacg atgagaacat atactatcct
    19801 agtatccgtg attttgggtt tgttggtttt gcaggggtgc aaaccaaccc agcagtcaca
    19861 gtccgaaaca attactcaag aactgacaac ctcgccattc agcaatggtg taccagccca
    19921 ggttgtagta attggcggac aggaatacaa gtatcccatt attgccattt gggttgaaga
    19981 tttaagagga aactttatag gcacagttta tgcatcgcaa tccatttcaa caggaatttt
    20041 ccgatatggg gtttacgata aagggaaatg gctgccgggt gagcgaaata ggccggctgc
    20101 attgccgcgc tggaaaaatt taaccgcgtc aaacgctcct cgcgttaatc agggggtcga
    20161 tgcgtttagc ggtgcaaccc cttccggttc atttgcttta aagaccatgc tgcctgctgg
    20221 tattgatgaa tttaaggtgt atatggaggt caataaaccg tgggacttta acaactactg
    20281 gcacaacaac cggtacccgg gcgatgagga gtatgccaca tcggggcagc cagcaatcat
    20341 atatgaggct ttggtttcta gaaaaacggg cgatagccta ttctatttca aacccattgg
    20401 ccatagcaac ccttcggggg ctaatggcga aattattccc gatatagcta cctttacatc
    20461 tgcgttaaaa atagtagaaa gagcctggat tgaactgtcc aaataatgga gaaaggtatt
    20521 gataacaagg ttgcagtaat tgcaggggga actggtctta ttggaaggag tctggttccc
    20581 atgctcctaa atgaagggta tcgggtaaaa ttgcttaccc gtaaccccaa aaaggttcaa
    20641 aaatctggta tcaatgctga tattatagaa tggagcggta aacccgatag cgaattaact
    20701 gccctgcttg agggtgttga tgttgttgtt aacctttcgg gctacaatat tgcaacgctt
    20761 ttgacaaaac gaaatagaaa gaggattgtt gatagccgaa tacttccaac aagggctcta
    20821 gcgcaggcta tgacatcatg ccgtaaacca cccaaagtgt ttgtccaagc ttcagctgtt
    20881 ggcgtatatc catgtaatag tagttctgtt ttaactgaaa aatcggcagt aggtactggt
    20941 tttttacctg cccttgtaag cgattgggaa aatgaggctt tggcttgcgc taatgttacc
    21001 cgggtgattt tactccgcac gggtatagtg ctggccagcg atggtggtta ttttcctaaa
    21061 attgcgaaac caatccgtta ctttgcaggc gcaatactgg gcgatggtaa aaatattgtc
    21121 ccgtggattc atatagccga ccatgttagg gcaataaagt ttcttatcga taattcggct
    21181 gcctcgggag catttaatct taccgcaccc actaccaatt ccaatgccga tatagttttc
    21241 caggttgccc atcactaccg ccgtccaatc ctattccgca taccatctat tatcctgcac
    21301 cttttaccgg gaaatatggc taacgaggtg ctattggcaa accagcctgt ttacccagaa
    21361 cgtttaaccc agttaggctt tagctggaac ctccctgtcc ttgaaaatgc cattgccgat
    21421 ttgttggcaa agtagattct attttttttc ttaccttagc gttttgaaac atttcggcat
    21481 tatatggata attttacagt tgatattcgt tcagccaaca tatttcagga ggataatctt
    21541 gttctgtccg atgttaactt ttcgctaggt aagggtgagt ttgtttactt ggtgggtaga
    21601 gttggtagcg gaaaaacaag tctaattaaa accattactg ccgaaatacc tctcaaagag
    21661 ggcgatgggc ttgtgtgtgg tttcaacttg aagaagctta aacgcaagca tattcctaag
    21721 ttacgccgaa aaattggtgt agtatttcag gattttcagc tgcttactga cagaagtgta
    21781 tacgataacc tgctttttgt gctaaaagca actggttgga aagacaagtc agcaataaag
    21841 cagcgtattg ctgaggttct ggatatggta gagctaaagt ttaaggatta taagatgccc
    21901 catcagctat cgggtggtga gcaacagcgt gttgttattg caagggcact attgaataat
    21961 cctgaactta tacttgccga tgagccaacc ggtaaccttg acatggagac ctcggaggga
    22021 attatgcaaa tactccataa cctctcagct tcaggaatct ccataataat ggcaacacac
    22081 aacaatcaaa tcataaagaa atttccagcc cgcaccatta agtgtgaaaa gggtaaggtt
    22141 gttgagaccg agcaaatgac cgaaattgat ttcgaaagtt tgatggatta gcgccactat
    22201 ataaatgcac agtcaccatc gccatccatt agttcaaaac tgtctcctat tgaatcagcc
    22261 atactgtcgg ccactagata tttacgggtt cgttttttgc tgtagaagta gtatggtcgt
    22321 agattaagca tggaattcac aatcttttga ttgtaaatgt tgataaatga tgctttggcc
    22381 cttgaagcat gcagcagtat caccttggcc ataacatctt cggtgccgtt ttcaaaccag
    22441 tagtaaggaa ctgtaaaacg ggtattgcta tgctgcatga gtaaaacacc aataagttca
    22501 tcatctttaa atacttttac catgtaatat gcaaaggcag gtatcaccga ggcaaaaaag
    22561 aacttttgtg ctgctctgtc gggcaaaagg ccgttaatca accacggata tcggataatc
    22621 caattaatct cttcagtgct tcgttttgta agcgtgttgg ctgtcgctag ggttagcttg
    22681 tttagtattt cgtcgtcggg tcttgtaaag tattcaatct caatattttt tggaatttta
    22741 agaaatttgc gtgtaaggtt tgagtggttc aacaagttta tgaacttttc ggttatatag
    22801 gtgatgggat tgctatggct gcagcagttg taagaactta aaagggtttt gcgcaggaaa
    22861 aacctttgcc cttcaatctt tttgacagtt ttaaatgttt tggtcttttc aaatacagct
    22921 tcggttattt ttgagaagtt agttgccatt aacattccct tccagtccaa taccactgcg
    22981 tcaattagtt ttgtggctat tccttgcctt cggaaactgg gatgaaccca tgctccacta
    23041 atccatgcaa atggtatcga ttggttgttg gcaaatgcaa tatcgggcaa tacagttagg
    23101 tatgcaataa gtttttcgtt ccagtgggct tggtaaagta cattgtcgtt tgagttggca
    23161 cggggatttg ccattaacga cattgctcta accttactaa tgggcaaaaa atctttctcg
    23221 tttaaacccg tatcatcatt gaaaagtttc agctccctta acgagatttt tgatatcgac
    23281 agcatatccc tatatgagtt taagtttttg cttgatgcta accaatgcct cacctgccgg
    23341 taaggagtta cgatggtaat taaaataagt acacggtttt gccccaaaac cactaaaaaa
    23401 tcgtgcaatt gataaaatca ttgaaccttc aaagtcaagt actttatcca ttccgcagtg
    23461 gttttttatt actccatcca ttaagtaaaa cattacacct tttttcttat tctcgggttc
    23521 ggctgagcca agcaggtagt gtaaggtgtt gtgactttca gcaaacaatg ccactgcaca
    23581 aagtttgtta ttcctatcag ttactccggc aatggttgct ttattatggg ttagaaggct
    23641 ctcaatgagt ttctcaataa caatgacatc cttttttcga ttaaccataa agccgtattg
    23701 cctgtaaaat ttcataaaat ctaccggatt taacgtggtg gtaagtgtaa actcgttggc
    23761 aatggcttta aaaatattcc ttttggtatt ttggttgtag tttctgaata cttgctcgtt
    23821 atagtttaat aaatcgagca tgtatgtttt tcgtctgttg atatgacccg tatgcttatt
    23881 ggttttatta tggatgttaa gatttagatc aacatacttg aactttgcgg gtatttggtc
    23941 aatgaataaa tctacaatat ctgccgaaat gtgatttgtt gagaatagcc ccaactgctg
    24001 gcagtgtaag ggttgaacta tgtagtttat tccatatttt cgttttattg gaagcggaaa
    24061 aaggtagctg taatcatttg ttgccagtgc cccccagttt ggagaaacca catccagata
    24121 ccatgagtat gcataaatac ttccattaaa ggcattgtca attgttgcat tccaacgctt
    24181 acagtcaatc tcctcgcgat gtaaaaaccc tatttcaaga tttttcttca ttcctgcaca
    24241 tttcaatggt tttttccaga atatccatga gcgaaatacc tgggttattt tgagctaagt
    24301 aatcgatgtg ccatacgctg acaaagttcc cgtttacatt tttaacagcc tgtaccaagc
    24361 gcttaactag ctcaaaggct tgattcgggt cgtagcggac aatgttaagc agcgatttat
    24421 ccattatggc aaaggggaag atttttagat tctgggttgt gttatcaaat aggtcgaaaa
    24481 aagggtaggg ggtacaggtt cctgccctaa atcctggctt atcggcatac cccattgagt
    24541 actcctgggt gattcctgct cgcattaggg ggtagtatga gtaaggtatg ctttgtcgta
    24601 gataatgaaa cctcgatgcc gatacagctt gtcctagaat atctactagt ttcgacagtt
    24661 cctgtcggaa aattgtatcg ctcagaaagg ttctgtatga tgggtgtata ccaattggat
    24721 agcgtgtcga cagcttgttg accagattta agaacgaagg gttactggtt tttacctgct
    24781 tatcgtattt gctccagttg ccaacatgta taaaccattt ggtggcttcg cctgctggta
    24841 gtttagcaaa taaatggtcg taaatatcaa aggggtcttg ttctttgttt gcaagcaccc
    24901 tgaatcgagt tttagccttt ttaaaatcga atgtaatggc tgatttggta aagcccagag
    24961 cgctcctgat tattcctttg cccttgaaag caaatgcgct gtccaggtca aaggttattt
    25021 ggctggtaaa gctacggtgt tttgagtgtg agttgccaaa cttttcctta ataactgctc
    25081 ctagcttgtg tgcccaaaca tcaacaacag gtatttccag aaaaccgttt ttgtatgcaa
    25141 tgctattagt aaacctgaaa cgtccgtgtt tatcgggctt gtggggtaag tactcttcgt
    25201 agcgggtaac caggtagaat gcgcctgcaa gcaggtcaaa aggaatatcg gctttatggt
    25261 tccaggtcat ggggaatgca ggcgtgccat tccattcacc tatttccatt ggaatttggt
    25321 tgattcctgt ttcaaacagt aaggttgttg gtataatact tattgagccg ggtatcggct
    25381 gtttatcgta gcttagtatt ggccctttgt attccgatgc ttcggtgtag gaactcgata
    25441 atcggtaggg catacggagt atgtccttga aaataaatcg gcaaatatat tctagcctcg
    25501 tacttgattg acggctgtaa ataagtaatt ccacaacctg ttattttgat tggcaatata
    25561 caaaatgcct ttcaattttc ataggctaag gcgctaaacg tcaaggtcaa ccaccgtaat
    25621 tcctgccccg ccaaactcca cgtgttcgtc gctaaagttt tttacgcccg gcgtagcctt
    25681 taaaaagtca cgaattacct gcttgagaat tccatttcct tttccatgta gaatccgcac
    25741 ccggttgaac ccgagcatca gggcatcatc tattaaatcc tgaactgctt caagggcctc
    25801 ttcggccctg tatcctcgta catcaatatc ggacttgaag tttaacctcc gcttataaac
    25861 gtcgaatcca gtgtttaaag ttggtttctg ctttgccgct tcacggtatt cgccagtaga
    25921 taatctctta agcttattaa ccctaactac ggttatcatt ccacctatgg caaccgatgc
    25981 gcttttatcg ctaatgctaa tcacctcgcc aaccatatcg cttccttcca ctttcacctt
    26041 atcgccaggt tctattgctc ttacttcatc ctcctttaca ggtttgcttg ttatggtctc
    26101 tttttgctga ttctttcgtg cttttctttc ctgccgttgc ttaatgagtt ccattttacg
    26161 ctcaattaga gcgttagcgg tttctggatt ttctatttcg atttttaaat tttctaacgc
    26221 ctttctggcc tcgcgcgttc gttccttatc ggcgttggtt tccttaatgg ttcttatggt
    26281 gttttcaatt tgtttattca cattggccag caattcctgc gcttcctttt ttgcagtagc
    26341 aataatctcc ttacgttttt tctcaagctc ttccagttcc ttttggagtt tttcctcagc
    26401 ttcttcggct cgtttattgg caagccttat gctttcgcgt ttcttttccc agtatcgttt
    26461 gtcgcgcgat atctcacgca gatgcttttc aaaggtgatg taatcggagc ccaccttctg
    26521 tgatgccaca tccaaaacct tttccggaag tccaattttt ctcgcaattt caaatgcaaa
    26581 gctgctgccg ggcttgccaa tttcaagttt aaacaggggc tcaatacgtt cgttgtcaaa
    26641 aagcatggct ccgtttacaa tgccaggtgt attggcggca aaatgtttaa ggttggagta
    26701 gtgagttgta ataaccccaa aagttccttt gttgcatagc tcttgaagga ttgcctcggc
    26761 tattgctcct ccagcggtag gttcagttcc agccccaaac tcatcaatta gcacaaggct
    26821 ttggccatcg gcatgcctaa gcatttgttt catgttgatt aggtgcgagc tgtaagtgct
    26881 taggtcattc tccaacgact gctcatcgcc aatgtcgatg aaaatctttg aaaatattcc
    26941 catttcggaa ttttccaaaa cacacggaag gaaaccgcat tgtagcatgt actgcagtaa
    27001 cccaaccgtt tttaaacaaa ccgatttgcc cccggcattg gggcccgata tcagtagaat
    27061 cctatcgttt aggttcagct caatatcgag cggaacaatc cttttcccct ctcttttaag
    27121 gctcagctgt agaattggat gtcgggcttg acgaaggtat attaccgttg agttactgtt
    27181 tagtatgggt ttgaccccat caaactccat ggctagtagc gcttttgccc tgatgaaatc
    27241 aatttcgccc atgaaatcgt aggcagtaag cagctccggc aggtacggtc ttatactatc
    27301 ggctagttca atcagtattc taactatttc gcgcctctcc tcgtactcaa gttcccgaat
    27361 ttcattgtta agttcaacca cttcaacggg ctcaatgaaa acggttttgc cggtagccga
    27421 ctcgtcatga acaatcccct ttagtttcct tttgtttgca gccgcaacgg gaataaccac
    27481 ccgtccttcg cggactgttg gctgtgtgtc ggcttcaaca actccttgtt cgcgagcgct
    27541 gcgcattatt ccttgcacta gcttgtttac cgatgcttcc tttgctctta tgcttgctct
    27601 aatttcggcc agctttgccg atgcattatc ctttattcga ccgtatctgt ccagaatact
    27661 gtcaatacgt tcaataacat aagggtagta gtttattcct gttgcaagct tcgcaattgc
    27721 tgggtattta tccgataccc ttttcacaaa tgcaagaagt tgctttatgg tatccaacga
    27781 gcgccgtagc tgttgtagct cattctcatc gagccattgc ccttccacat taagtttacg
    27841 tagggctgga gtaacatcaa tatagccatc ggtggggaag ccatcctcca tctggcagat
    27901 agtcttcatt tcatgggttt gctcaatcca ttcaaaaaca atgtcaggat tggtgttgaa
    27961 tccggcatcc cttactcgct gttcgccaag ttcacataga cagcgcgcaa gcgttaactc
    28021 gcgaacctta tcaaatccaa ccttttgctc aaaggtatcg gggtaaatca ttgtaaatgc
    28081 aaaatgttag tttgtgcaaa gttagcgatt tgataccgat gtggatgaaa atttgttgct
    28141 tgtagtatag aaaataactc attctatttc aatgttgttt agttaacaaa aatatacatg
    28201 tctgacatct tgcacgcagc gaatgatctc taatcgaccc catgagacgt ttcacttgtg
    28261 ctcaacatga caatagcttc acgaacaacg attcacgaac aacgaacacc gcttctagcc
    28321 ccgtaggggc taaacgtttg taaacctatg tatactccca cttgtattgc gatgcacgca
    28381 atacattttc caaagagcaa gggtgaagct ttgcatcgca aaggaaaaac attgcaaaga
    28441 gctattttaa agttctttct tgtcttgata caagaaagaa ccaaagaaaa tcaaggctga
    28501 aaagcctgac ccgatgggtg ccccgtgggt ggaactgcta cgcgatacta ttcgccacgc
    28561 ctgcggcgtg actcatgtgg tatcgcttac taaacccacc gccatgttcc acccccgacc
    28621 cattgccggg tcaggctttt atgcctctgc ttgcttcttc cgatccatcc cctctcaagg
    28681 aaaagggctg gggagaggtc ttttccccca aacaacaatc aacgctctca gccccgtagg
    28741 ggcgaaatat ttgtaacacc aagattattc ctacatgtat agcgatgcac gcaatacttt
    28801 ttccaaagag caaaggtgaa gctttgcatc gcaataaaaa agcaccgcaa agagcagttt
    28861 ccaaaaaatt aacaagtatt agagggttta aggcaaagag atacttcggc tagctcagca
    28921 taacaaggga gcagaaagga agatagggtt tttgtcatcc ctagcaaagt cgagggatct
    28981 attcaaagga ttgaagaaaa tgagcaatga gatgctttgg cgaagtctat ccctataaat
    29041 cgggactcag cacgacagcg taatatttcg ctttcgcgca acatgacaaa taacctttac
    29101 tatacaacat tgcattctat ttaaggttaa tgtattgaaa ggctataggt ttcagttgtc
    29161 aaaacctgaa ttgttatggt ttagaatgca gttgcgattg taatcctgag ttattcatgt
    29221 ttcggatacc attatgttaa tgctataatt tgatgttgat tttttttata acttacacat
    29281 caattagttc aaatcagtag ccaacatttt aaaaaactat ctatggactt cataattagt
    29341 ttacttatca gcactctagt ttcgggagtg gccgctgcaa ttctccttag cagagtttat
    29401 aagaatagta tatttgtaaa agttggcatt gtttggctaa taaacctgct gttcatcatg
    29461 acaacggtga gcatcaggat aaaattttac gatggcgaca cattgattcg ttacgtgatg
    29521 cttgccgtta acgtgctttt cagcgtgctt tgcttttatt acgcaaccat aaaagtggtt
    29581 cgtccactat caagggcaat tgataagctt tacaagctag ccgagggcga ttttaacgtt
    29641 cccgatgacg atacaaatgt taaccccaaa accgatttgg gtcagcttat tcaggctacc
    29701 agtatgataa aggataactt tggacgagtt gttttgcaaa tccaaaacaa tgtgagcagg
    29761 ttacgcgaca tgagcgatga gctaaacact atttcaagcc aattggctga aggggctgct
    29821 gaggaatcat cgtcaattga ggagatatca tcgtccatgg agcaaattgc tgcccatatt
    29881 gaaaggaatg ccgataactc taagcaaacc gaggtttact cgcgcaaggt tcttgatggc
    29941 gttaaaaatc tgaacaaatc ggctgaggag aatttggagg ccatcaacca cattgctcaa
    30001 aaaatcaatg ttataaccga tatagccttt caaaccaaca tacttgcact taatgcagcc
    30061 gttgaggcag cccgtgcagg tgcacatggc aagggctttg ccgtggttgc tgctgaggtg
    30121 cgtaaacttg ctgaaacaag caagctggcc gccgatgaaa tagttcagct agcaggtaaa
    30181 agtgtgaatg ttgcccagca tactcaggtc atgcttaacg agcttattcc tgatattaca
    30241 cgaaccacca acctggttga ggagattaca tctgcaagta ttgagcagca cgtaggtgtt
    30301 gagcaggtta ataatgcaat tcaacagcaa aatgttgttg cccagcaaaa tgctgctatc
    30361 agcgaggagg ttgttacaac cagtaataaa ctttcagagt ttgctaacca atttgtggaa
    30421 ttggtatcgt attttaagat tcggaagaaa tagattctgt ttgttgattc atcaactgta
    30481 tttttcaatt aaagaatttt ggtttaacta ttaacttttt acaattcctc taacttcctc
    30541 caacttcctc atattctcaa tttttctttt aacttttaac gtttaacggt tcacgtttaa
    30601 ctgactcaaa cttcctctaa cttcctctaa ctccatcaca ttctatctat tccgactcat
    30661 tccgattcat tccgattcat tccgattcat tccgactcat tccttaaaat tcatgtcatt
    30721 tggtgtaaat gcatttctta ctgctccaca tccctcattg aaagttgaat cctttttctg
    30781 ggaatatcaa cctcaagtac tttaaccttt acgtgttggt gtaacttaac aacatcaagc
    30841 gggtttgaca caaagcggtt ggccatttgc gatatatgta ccagcccatc ctgcttaaca
    30901 cctatatcaa caaatacccc aaaattggta atgttggtaa ctattccggg cagtaccatc
    30961 ccgggcttaa ggtcatcaat gctaaaaata ccttcggcaa actcaaatac cttgatagct
    31021 ttacgtgggt cacgaccggg ctttgagagc tcctgcatta tgtcgtttag tgtgggcagg
    31081 cctaccttgt cggttacata tttttcaagt ttgatttttg cccttagctc ttcattcctg
    31141 attaagtcca ctactgagca gcccaaatcg gctgccattt tttcgacaat atggtagctt
    31201 tccggatgaa cagctgtgtt atcgagtggg ttttcagcct cggcaattct aaggaatccc
    31261 gcacattgtt caaacgcctt ttcgcccagg cgtggaaccc tttttagctc atctctgctt
    31321 ctaaatgcgc cattttgggc tcggtattca acaatgtttt ttgccagctg gggtccaagc
    31381 ccggaaacgt acgttagtag gtgcttgctg gcggtgttca ggtgaacccc aacgtggttt
    31441 acacagcttt ccacaacctg gtcaagacta cctttcagct tggtttggtc aacatcgtgc
    31501 tggtattggc ctacgccaat cgactttggg tctatcttaa ccagctcagc caacgggtcc
    31561 attaggcgac ggccaattga aaccgctccc cgaacggtta catcgtattc cgggaattcc
    31621 tcgcgagcca ctggcgaagc cgagtatacc gatgctccat cctcgctaac aacaaaaacc
    31681 tttacatcgc ggttaaacct aattcgttta atcaatgcct cggtctcacg acttgctgtt
    31741 ccgttaccaa ttgctatggc ttcaatctta taggtatcaa ccaatgccga tatcttactc
    31801 atggccttcc cttgctcgct ttggggtggg tgtggaaaaa cggtttcgtt atgcagtagg
    31861 tttccctgcg ggtcaaggca aaccaccttg catcccgttc ggtagcctgg gtcaatggca
    31921 agcacgcttt tttgaccaag gggtggcgag agtaagagtt gcctcaggtt ttc
//
LOCUS       DAOOWJ010000019        31791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1435_length_31791_cov_8.213669, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000019 DAOOWJ010000000
VERSION     DAOOWJ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 31791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31791
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1435_length_31791_cov_8.213669"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(545..772)
                     /locus_tag="PLW22_03370"
     CDS             complement(545..772)
                     /locus_tag="PLW22_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73776.1"
                     /translation="MNTQKNPTLQKIKAREMQYLEPLNATDMLNINGGTWLSYWLGYI
                     LSSIDNNVPNEDQTRYGGAYLETISGRSSIM"
     gene            complement(779..964)
                     /locus_tag="PLW22_03375"
     CDS             complement(779..964)
                     /locus_tag="PLW22_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73777.1"
                     /translation="MEQLSLNKFGVQQLNAKEIVEIEGGNPLLWLLAGVIVSELLDRN
                     APADFWEGWNDAKQNKK"
     gene            complement(977..1351)
                     /locus_tag="PLW22_03380"
     CDS             complement(977..1351)
                     /locus_tag="PLW22_03380"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOU73778.1"
                     /translation="MKFFEYLVRIEKMHCLIKEQRTGTPDEFAAKLGISRTRLYEVID
                     ELKSRGAPIVYSKSRETFFYEYSFDISLKCTMKPLSRKELVEKNGGASLPFLFSGRNA
                     PNFATVTLQKHNQIWMAKLLTF"
     gene            complement(1359..3518)
                     /locus_tag="PLW22_03385"
     CDS             complement(1359..3518)
                     /locus_tag="PLW22_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOU73779.1"
                     /translation="MRVKQRDITDCGAACLASVAAHYKLNLPIAKIRQFAGTDRKGTN
                     VLGMIEAAQKMGFHAKGVRGDFDSLFKIPKPSIAHVVVKEVLQHFVVITKTTKKYIEV
                     MDPGDGRFHKIPHEEFKKSWTGVLILLVPGETFKPKSEKVSSSFRFWNLIKPYKGVLT
                     QAIIGAVLYAVLGLSTSIYVGKIVDYAIPSNNANLLNLLGIAMLIIIGVRFLLNIFQS
                     VFVLKIGQRIDATLILGYYQHLLRLPQSFFDTMRVGEIISRINDAVKIRLFVNEVTVN
                     LIVNVLILIASFMLMFTFYWKLAVIMLLVMPLYIITYMIINRLNRKVQRSIMENSAEL
                     ESHLVESLNSISTIKRFGLEGYSNLKTEMRFVNLLKPLYKSGINSIYSNNVSGLISQV
                     ITIVLLWVGAGFVLESTITPGELLSFYSLIGYLTSPVTSIIGFNKTYQDAKIASDRLF
                     EIFDLEVEDEKNKIEFTAEHVGDITFKNVDFRYGTRVEVFKALNLHIPKGKVTAVVGE
                     SGSGKTTLTSLLQNIYPLQSGNIYIGKFDLRYFTNESLRDAVVAVPQNIDLFAGTIVE
                     NIAVGEFNPNIQRIIEICTQLGMIEFIESLPNRFNTYIGEHGTSLSGGQKQRIAIARA
                     LYKNPEILILDEATSSLDSIAESFVQHTVELMRQKGKTVIVIAHRLSTIAMADNIIVL
                     DNGKVVEQGVFKELIHSNGQFCRMWQHQTVEKHIIEN"
     gene            complement(3524..4702)
                     /locus_tag="PLW22_03390"
     CDS             complement(3524..4702)
                     /locus_tag="PLW22_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOU73780.1"
                     /translation="MTELKRVFPGFVIQNSVEGYLHSISKRSKKIYWLIIMIIIGTLA
                     SMPFILVDVTIRTSGLIKPSIEKQNIVLPYGGKVIYTKLKNNLRVSAGDTILVIDTIP
                     ISSKIKTLEFNLGELKSYQNDLGLLIEHNFLLQKFNPKSLVTSKYMLEVSSFQRQIST
                     QSNRIEKLKKEHSRNTLLYKQKIIAEVDYETSKYNLDNEILLRNQFISQQLASWQASL
                     AEITNQIPKIESEIETAYNELSKHFITATLSGTIQQCNELQVGSIIYANQIIAELSPD
                     CELIAVCYVQPSDIGFIRVNQDVKIQVDAFNYNQWGLLPATIKEVSDDIVSDGQNNMY
                     FIVKCKLSKNYLTLQNGFKGYIKKGMTISSRIIVTERSVYDLLFDKVDRWFNPYTEMK
                     "
     gene            5117..5734
                     /gene="yihA"
                     /locus_tag="PLW22_03395"
     CDS             5117..5734
                     /gene="yihA"
                     /locus_tag="PLW22_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOU73781.1"
                     /translation="MVIRTAKFVASYPSVQKCPKTDLPEYAFIGRSNVGKSSLINMLT
                     CTKALAKVSQTPGKTQLINYYLINDSWHLVDLPGYGYAKVSKGEREKFSDIVLNYILK
                     RENMHLLFVLIDSRLEPQDIDIQFINWLGVNQVPFALVFTKTDKVSSMVLEQNIELHK
                     KVLQESWEELPPIFTTSCVTRTGREDVLNYIDQVFREAKITHCQV"
     gene            5790..6734
                     /locus_tag="PLW22_03400"
     CDS             5790..6734
                     /locus_tag="PLW22_03400"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOU73782.1"
                     /translation="MHSKHQIKFFTGRSSRYLAERIAQSFGIPLGKSTLTVFSDGEFQ
                     PSFDESVRGCTVFIIQSTFPPVDNLFELLLMIDAAHRASAYKVVAVMPYFGWARQDRK
                     DKPRVAIGAKLVANMLVAAGADRVMTMDLHADQIQGFFDVPVDHLYASSIFVPYIKSL
                     NLENIAIAAPDMGGAKRANAYSRFLNSSLAVCHKNREKANQVGEMTVIGDIEGKHVII
                     LDDMVDTAGTLTMAADLMMGKGAASVRAFATHPVLSGSAYERIRDSKLTELVVTDTIP
                     LRTDKDTSKIKVLSVAELFADVINKVYNYQSISEKFII"
     gene            6864..7439
                     /locus_tag="PLW22_03405"
     CDS             6864..7439
                     /locus_tag="PLW22_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOU73783.1"
                     /translation="MKTIELSAAPRTETGKKAAKHSRKSELVPAVIYGAGENIMISLG
                     EKELKKVIYTPIVYLVKLNVDGKMHDAIIKEVQYHPVTDKILHVDFLKVSENKPITIG
                     LPVNLVGQAEGVKAGGKLLQVVRKIRVKGLAKDLPDAISIDVTHLGIGKSIMVAELSF
                     DKFTVVEPKSMVIATIKSTRAAREAQQEQAK"
     gene            7451..8014
                     /gene="pth"
                     /locus_tag="PLW22_03410"
     CDS             7451..8014
                     /gene="pth"
                     /locus_tag="PLW22_03410"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOU73784.1"
                     /translation="MKYLITGLGNIGSEYSNTRHNVGFMVLDALAKASNITFADARYG
                     FVAQLKHKGRTFVLLKPSTYMNLSGKAVNYWLQKENIPLENLLVVVDDIALPLGAIRL
                     RPKGSDGGHNGLRNINDVLGTQDYARLRFGIGNGFSRGRQVDYVLGEWTDEELKVLPE
                     RLTLAGEAVLAFGTIGIERSMNLYNSR"
     gene            8025..8450
                     /locus_tag="PLW22_03415"
     CDS             8025..8450
                     /locus_tag="PLW22_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOU73785.1"
                     /translation="MRVDKWLWFVRVFKTRTLATEYCRKGRVTRNGIEVKPSQDINVG
                     DTLKVRKPPVTYTFRVKDLPKNRLGAKLVESYMENLTPPEELQKLDPSFMAFNIHRER
                     GTGRPTKKERREIDELFDSGFGGFDWDSFEPDADDESNE"
     gene            complement(8509..9027)
                     /locus_tag="PLW22_03420"
     CDS             complement(8509..9027)
                     /locus_tag="PLW22_03420"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOU73786.1"
                     /translation="MEPKNYKFISPFELNENTFDLLDNQWMLILAGSKDKHNSMTASW
                     GGFGILWNKPVAFVFIRPQRYTLQFVEQNDYFTLNFFEEKWRSALELCGSKSGRDIDK
                     TKAAGLTIAESLKAIAFQEARLVIECRKLYIDDIKPENFLDKEIISKHYPKSDFHRLF
                     IAEITSVFKKEG"
     gene            complement(9045..9917)
                     /locus_tag="PLW22_03425"
     CDS             complement(9045..9917)
                     /locus_tag="PLW22_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOU73787.1"
                     /translation="MTREELVKHAKLFQREEITGYQVYKNLAKSTNDKNNKTVLAKIA
                     GEELKHYNIFKQISGVDVAPQKTVVWFFTICGKILGLTFALRLMERQEAKIQDVYERV
                     IPYMPEAEAVKKSEEEHEDQLIALIQEERLDYAGSMVLGLNDALVELTGALAGFSLAM
                     QNTRLIAVAGLITGIAASLSMAASQYLSVKTENDDRHAGKSAIYTGLAYIFTVAILIS
                     PFLLLTSYMLALATTLLLAIAIIFAFNFYIAVAKNLNFKHRFLEMVVISLGVSALTFG
                     IGLLVRNYLGIPID"
     gene            complement(9928..10674)
                     /locus_tag="PLW22_03430"
     CDS             complement(9928..10674)
                     /locus_tag="PLW22_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOU73788.1"
                     /translation="MFTLEVCANSVESAINAQKGGANRIELCENLYVGGTTPSFGCIA
                     ETLNQLNIPVHVLIRPRPGDFVYSDMEFRQMLRDIEACKKLGVKGVVSGILRANGTLD
                     KERTKELVWAAKPMTFTFHRAFDIMPEPFKALNEIIECGCNFLLTSGQKNKAVDGLDL
                     IRQLIAEGGNKIALIAGGGINHSNILELAQNGVNQFHMSGSEIVARYNTNHTGLSFVS
                     ALLPENTVQQTNAETIASTVAKLKQYFNKK"
     gene            complement(10678..13326)
                     /locus_tag="PLW22_03435"
     CDS             complement(10678..13326)
                     /locus_tag="PLW22_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU73789.1"
                     /translation="MKKIVTISALLLIILSSCDTILNRHLIKGSDKRKEVHAQFEERV
                     KLIAAYDSSIYKIINSELTTKEREGFEFLYAYMPLSDLAMHSPEYVLKNVQLALKARK
                     EFKWARKVPADIFLHFVLPYRVNNEYTDNAREIFFEELKERLLGLNTYQAALEVNYWC
                     HEKVTYQSTDERTCGPLTSVRSAIGRCGEESTFAVAAYRSVGIPARQVYTPRWAHTDD
                     NHAWVEVWVDGKWHFLGACEPEPELDRGWFAGPAKRAMMTRTFVFGKYNGPEEQLKQT
                     NYYTEINLMPNYAPTRKLTVTVTDSTGNPVEGAKVEYQLYNYAEFYPIATLQTNANGL
                     CTLTTGIGDLMVWAQKDNTYAFSKADRNQNDIYLTLKKEIPFTNLSLTLTPPDEQTIP
                     PYDESTADEHNRRVKQGNEIRSNFISTFIDSASAANLAMEKGISIDETWKWLSKSRGN
                     WNEIYSYIKNLDTKDISVGVALLSTLREKDLRDITTDVLFDHLSRIDSFPALLESRMV
                     KEFDSYILSPRIGREFITPWRSFLQDAFTFEEIGFFRNNPQNIAQWIKEFVTLDTESN
                     YYRVPLSPESVYRIRRADEYSMAIFFVAACRSFGIAARLEPASKKPQYFMKGKWIDVN
                     IADVKETQATTPTGMLIINLDKNSAVEQPKYYTHFTIARFEKGKYSTLDYEESPLFNK
                     FPAALELPEGYYRIVTGNRHKSGAVNCNVYYVRVESGKKIQFTLTIPSTQTEKMVLGT
                     ITTDITIPFIQNPQTIEQITKDKPAIVVIIDPLAEPTKHLLRDISSLCSEFSGIGNSI
                     ALILASGKSAKTDLTSIFDGKPYPNTITLGNDINGQFEAKVGQAVGKQLEYPVVLVVN
                     NNGEIVYHSSGYSINLGEQLLSALKQ"
     gene            complement(13542..14564)
                     /gene="recA"
                     /locus_tag="PLW22_03440"
     CDS             complement(13542..14564)
                     /gene="recA"
                     /locus_tag="PLW22_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOU73790.1"
                     /translation="MEATENKERKEINKEKLKALQSTIDKIEKDFGKGSIMRLGDKAV
                     SEVPVISSGSIALDVALGVGGYPRGRVVEIYGPESSGKTTLAIHAIAEAQKQGGLAAI
                     IDAEHAFDRTYAEKLGVDVENLLFAQPDNGEQALEIADNLIRSGSIDIIVIDSVAALT
                     PKAEIEGDMGDSKMGLQARLMSQALRKLTATISKTNTCCIFINQLRDKIGVMFGNPET
                     TTGGNALKFYASVRVDIRKLNQLKDGEESTGNRVRVKIVKNKVAPPFRKAEFDIIFGE
                     GISKTGEIIDLGVELNIIKKSGSWFSYGETKLGQGRDAVRQLLLDNPELSNEIETKIR
                     ETLKAQ"
     gene            complement(14565..15035)
                     /gene="bcp"
                     /locus_tag="PLW22_03445"
     CDS             complement(14565..15035)
                     /gene="bcp"
                     /locus_tag="PLW22_03445"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOU73791.1"
                     /translation="MATTILKPGDLAPEFIGKDQDGKTIKLSDFKGKKLILYFYPKDN
                     TSGCTAEACSLRDGYDDLQSLGMEVVGVSPDSEKSHQNFIHKYNLPFRLIADVDHAIA
                     EKYGAWGEKKMYGKAYFGILRTTYVINENGTITHVFNKVDTKEHVKQILSEINK"
     gene            complement(15347..15982)
                     /locus_tag="PLW22_03450"
     CDS             complement(15347..15982)
                     /locus_tag="PLW22_03450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU73792.1"
                     /translation="MAPIPKIVRWIELAALFVGLPVAFYFNNLKLPKSIPLLVVFLIA
                     LFYLLTTKSFSKKSFGFNGYRSWRTLSLRILISLLAISLLSFIFLPSEQLFYLPLHRT
                     ELWLLIMFFYPIWSAFTQEVIYRGFFFHRYTPLFKNDKIVITLNGILFGLLHIIFRNW
                     IAVAGATIIGFVWAYSYFKHRSILVVSLEHAIVGNFLFTIGLGYFFYVPDF"
     gene            complement(15995..17182)
                     /locus_tag="PLW22_03455"
     CDS             complement(15995..17182)
                     /locus_tag="PLW22_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73793.1"
                     /translation="MENEVKQAYLKYFEDYVQRKLEDTLNAFSPDFNMIGTGVDEFSV
                     TYQHSTELFRREFAQAPEPLAYQFKSLKVCPITADSAYVMGLVDMKFLLPTGEFEYTN
                     NRTTAILRKEKGEWKIIHGHWSQPTEMQQEGESVPLSAVLKQNRELKNQLIIKQQELE
                     KQNQQLKEMNQSLSKLFSIVSHDLKSPFNAFLGITDLMLMNFEDDFENKEYFQLRLQL
                     LNDLAHNLYDLTENLLNWAKLNSNAIEPKYREIPIDSLIQKQVNVLRPIWQKKRITLT
                     EEVPSELMVNTDPDMLAVIIRNLVSNAIKFTYPEGEIKIQVSNEPTILSIKIVDNGIG
                     MTAEQLKNLFKNYQSSRGTDNEKGTGIGLFTCKELTNKLKGNLKFISEPNKGTTVIVE
                     LPK"
     gene            17498..18571
                     /gene="carA"
                     /locus_tag="PLW22_03460"
     CDS             17498..18571
                     /gene="carA"
                     /locus_tag="PLW22_03460"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOU73794.1"
                     /translation="MNQQQKAKLVLEDGSVYTGYSFGYAGNTSGEVVFNTAMNGYPES
                     LTDPSYFGQILVATFPLVGNYGVPEPNPLGGIEQNWESGRIHIKGLVVCEYSQNYSHW
                     NANKSLGQWLNENKIPAISGIDTRKLTKQLREKGCMLGKIIVDGDTDWYDPNADNPVA
                     HVSVAKPITYGNGKKTIVLVDCGVKNNILRCLLRRDVQVIRVPYNYNFLSQKFDGVLI
                     SNGPGDPKVNIETIDNIRRLIEMDKPVMGICLGNQLLALASGANTYKLPYGHRGHNQP
                     VRLKGTDRCFVTSQNHGFAIEPSTLSDEWEVMFENLNDGTCEGIRHKAKPFFAVQFHP
                     EASGGPNDTEFLFDEFINLIDNK"
     gene            18583..21816
                     /gene="carB"
                     /locus_tag="PLW22_03465"
     CDS             18583..21816
                     /gene="carB"
                     /locus_tag="PLW22_03465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOU73795.1"
                     /translation="MPHLRKYSKVLVLGSGALKIGEAGEFDYSGSQALKALKEEGIAT
                     VLVNPNIATVQTSQDVADTIYFEPVKPETVRQIIEKEQPDGIFLSFGGQTALNCGVEL
                     YQNGTLREFNVEVLGTPVSTIINTEDRELFVKELNQIGVSTPRSIAVNTVAEAVDAAH
                     QIGYPVIVRAAFTLGGQGSGFCSNPQELKALATNALTYAPQILVEESLKGWKEVEYEV
                     VRDRFDNCITVCNMENFDPLGIHTGESIVVAPSQTLSNSEYHKLRALSIKIVRHLGIV
                     GECNVQFALDPSSENYRVIEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLFELKN
                     SVTHKTTAFFEPALDYVVVKIPRWDLAKFKGASRQIGSAMKSVGEVMAIGRSFEEAIQ
                     KGLRMIGQGMHGLVANQHFNPSDLKLMLENPTDQRIFAIAQAFEQGWTAEDINAITRI
                     DSWFLNKLQGIVELSRHLAGYSAIEQVPSELVVQAKRNGFSDFQLARIIYNPSNNKIE
                     QYQQQVRNYRKSCGIVPCVKQIDTLAAEFPSETAYLYMTYHGNEDDTSVKPKPKVLVL
                     GSGAYRIGSSVEFDWCTVSAVKELRRLGFEAIVVNYNPETVSTDYDVCDKLYFDELSF
                     ERVMDIVEVERPLGVIVSVGGQIPNNLAGRLSKAGVMVLGTSPVSIDMAEDRQKFSAL
                     LDKLGIDQPEWQELTTMEQVQEFVNRVGYPVLIRPSYVLSGSAMNVVSNADDLNEYLT
                     VAAEVSKAHPVVISEFIEGAKEIEYDAVANNGRIFVDAISEHIEFAGVHSGDATLVFP
                     PQRLYLETIRRIRRVAAALAAELKITGPFNIQFLAKNNQIKVIECNLRASRSFPFVSK
                     VMNFDMIAVAVDFMMGVAPKTNPPSMLDIAHVGVKASQFSFGRITFADPVLGVEMAST
                     GEVGCIGQSVNVALLKAMLSVGHRIPQGSILVSSGPLRSKLSLIDPIARLARRGFVIY
                     ATRGTASFLNDHGIPAIAVSWPDEPENAPNSLNLIQNRLVDLVINIPKNLSRSELDND
                     YTIRRSAVDYNIPLITNARLAAAFIEAFLNTDNIQMEVKSYNELVGS"
     gene            21895..22695
                     /gene="zupT"
                     /locus_tag="PLW22_03470"
     CDS             21895..22695
                     /gene="zupT"
                     /locus_tag="PLW22_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOU73796.1"
                     /translation="MSNILIAFGLTLFAGLSTGIGSAIAFFSKHTNTKFLSLALGFSA
                     GVMIYVSFIEIFSKARESLVDAIGLHLGSWITVASFFGGMAVIALIDKFIPEQENPHE
                     VMLVEDAESGRKRKGKLMRMGLFTALAIAIHNFPEGLATFTAALSNPNLGIAIAVAIA
                     IHNIPEGIAVSVPIFYATGDKRKAFRLSFLSGLAEPIGAIVGYLLLMPFINGVTFGIL
                     FAAVAGIMVFISVDELLPAAREYGEHHLSIYGLIGGMIVMSISLLMFI"
     gene            complement(22756..23361)
                     /locus_tag="PLW22_03475"
     CDS             complement(22756..23361)
                     /locus_tag="PLW22_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOU73797.1"
                     /translation="MINPKDWLEFGQKFHGHKCPAMPMGLRVGAAAMNALGVERAKDG
                     QLIALVDLGDDHCATCYADGLQVILGATFGKGNIKKTHKGKWAVTVIDKATGRAVRVT
                     PKAEAMLANKQTSFFKDYREKGIPASKVPDLVVDPLVNNVMNAPDEKLINISEVFQYD
                     LKEHPHSFNSFVCEECGEMTVMEYGRIKGDKKVCIDCANKY"
     gene            complement(23380..24270)
                     /locus_tag="PLW22_03480"
     CDS             complement(23380..24270)
                     /locus_tag="PLW22_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU73798.1"
                     /translation="MSHDHNHHKVSGKNLGITILLNLFITVAEFLGGIFSGSISLLSD
                     AAHNFSDVLSLIISYTANRLSGQKATLTRTYGLKRSEILAALINSVTLLGLAIAILLE
                     SVKRLYKPIPIDSGWVIWLSLLSILVNGLSVLFIKADSRNSINIRSAYLHLFSDMLTS
                     VAVLIGGLAMKYLQWYRVDALFSILIAIYLLFTSWKIIKASLRIFMQFSPENININEI
                     TKQVESLEGVKNLHHVHIWQIDEHEIMFEAHLDVTNNITISDFEKILEQVKILLAKQG
                     INHVTIQPEYSVTDNKQLIH"
     gene            complement(24282..25034)
                     /locus_tag="PLW22_03485"
     CDS             complement(24282..25034)
                     /locus_tag="PLW22_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOU73799.1"
                     /translation="MLFIIVFIVISIASFIFSMLGLGGGMVYVPVLNWAGFDMVTVAI
                     PLGLLLNGLNTALVLIPFARKKMVDWKGGAVMAATALVASPLGAMTSEHVPVQLLKFL
                     FAVMVVAAALRTLWASKQEEPETMMSLKKRSIIGFFVGGFAGFIGGMLGLGGGFIIAP
                     ILMMMGYKTKEAAATTAFVVTFSSFSGYLGHVSQGHINWPLTIVVVLAVIIGSQLGGL
                     YMTQKAKSKQVKVVYAVVLLLIATKMVYGILS"
     gene            complement(25082..25669)
                     /locus_tag="PLW22_03490"
     CDS             complement(25082..25669)
                     /locus_tag="PLW22_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU73800.1"
                     /translation="MRQPTEVRQEQIKQAVLGIIYTDGLKNLSTRMLAQRIGMSEGAI
                     FRHFPTKQSIIHSIITDVQRDFIEHLRKIATSNELPQKRLEHLLCFTVKYLTDNKGIT
                     LLLFSEASHNNDEELKQALMQIFNTQKKMVSKVVLDGIAEGIWDENIPVENVAMLYMG
                     IPVSLNVELILNKGVLDTSNFCSRMMFLINKMLTR"
     gene            complement(25762..26151)
                     /locus_tag="PLW22_03495"
     CDS             complement(25762..26151)
                     /locus_tag="PLW22_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU73801.1"
                     /translation="MKRLVIYLTITAFFIAALSSNTFAQTKSQTSKTERKYKVTFIEL
                     GSVRCIPCQQMQPILKSVEEKYGKQVKVIFYDVWTDDGKQYAKKYNINLIPTQIFLDE
                     NGKEYFRHEGFFPENELIEVLKKKGVK"
     gene            complement(26319..26855)
                     /locus_tag="PLW22_03500"
     CDS             complement(26319..26855)
                     /locus_tag="PLW22_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOU73802.1"
                     /translation="MLLQILIGLAIGLIFGYALQRTGVTKYPRVTGMLLLKDFKILKF
                     MLTAVTFSMIGFYMLGNLGVIELHPKALDWGKVVGGLIFGTGMGLLGYCPGTLFARIG
                     EAKKDAWVALLGMLLGILFFAVNVKFFKSNLMTKNINGDISAVLGINQWIIVPIAALV
                     FVAIIYLANKVDDFKKNF"
     gene            complement(26858..27406)
                     /locus_tag="PLW22_03505"
     CDS             complement(26858..27406)
                     /locus_tag="PLW22_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOU73803.1"
                     /translation="MKIKLTQNGEWHWLTGGIVVSLLSIATYVLFEKLSYKDYPFGIT
                     AGVAYIAASFKYVFVSLADNPVIQKNWTSPAAFVEFIMLFGIILGAYIGAKLSKNYSP
                     EVIPAVWEKYHGTNVWKRFGVVLLGGFCLGLGTMLAVGCTTGNILQGWAHLSLGSMVA
                     GACFFIAGIIVAKLLYPKAGGR"
     gene            complement(27461..28855)
                     /locus_tag="PLW22_03510"
     CDS             complement(27461..28855)
                     /locus_tag="PLW22_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOU73804.1"
                     /translation="MLRLYFKNKVRIIFSLTCLACQLVLPTFSNANTGGDSTLLEFNI
                     TLRERFEVWDGMNAKNYGDPNGVGSLNDKILFQRIVAGFTFKPTSKIDFAAHIQDSRA
                     FGWSLRDSEYPELFRVKQKNTQEPYYTMNPNEEFFEIYDLYFSVKEIFKGVSLKIGRQ
                     KISFGDKRIFGPGEWGNTGRWTWDAVRLSYRNKNNSLDVFGGGTKTHDPEKVAIPFTE
                     TEHWGGGAYAHIQFGKLAVVEPFYAIKTQGSADYIRKQSFSKHWIGTRVFSDEVNRFK
                     YDFTLTQEFGHENGKTINAFGIIGLLGYQFHGLPAKPTLCLRETYASGGNNDDTRVRT
                     FDPAFGAKDSYYGRMNITSWSNLDDREVVLYLTPSKKFKIEMNYHFFYLPSPDNKTLL
                     GTMKLQAGKHHLGNEFNFFASYNAGKQFQFVGVFGYFWAGDLQPINGTTAQNSNWFAI
                     QAFIKLDKEIHAKH"
     gene            complement(29012..29386)
                     /locus_tag="PLW22_03515"
     CDS             complement(29012..29386)
                     /locus_tag="PLW22_03515"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOU73805.1"
                     /translation="MEDNKKSGCLCGVDEYMVLACSGACDLGQITDLVARKLRDNGVR
                     KMNCLAVVGAGIEQSIEAFKSRNILMLDGCPTDCGKRILDRHGFTDYKYFRVTDLGYK
                     KGQTPVTEEVVNKVYEKVEVIY"
     gene            complement(29399..30472)
                     /locus_tag="PLW22_03520"
     CDS             complement(29399..30472)
                     /locus_tag="PLW22_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOU73806.1"
                     /translation="MKPALLAALVFFSKCIVTAQTSDSTFKPNGKPIIQVFGNFDYNA
                     TNNAKKTYGFWFGRAHFGYEYQFSKQISGKIIIDAGRPTTVGQIEVRDTSGNSLDVTD
                     KSKEGSYYTMTLKFASIEWKPNSFVRIQAGGVLQNHYITQEKFWGYRYVAPTFQDKYY
                     GIPSSDLGIIGYFTLNEMLGFDIAITNGEGFRFDQDEYGDVKIAGGIDFNPIKGLQTR
                     FYTDYTKSDNPLKPSEQQLFSIFAGYKFDDKFRIGAEYNYRKNHLNINKHNLYGLSVY
                     GGVSVAKNVELFGRFDQLQANTISGDAQSWYYSGIGNAYIAGIHYNPVKGVNISLNYQ
                     GWKPLDSSVDFQNHIVLSFEYKL"
     gene            complement(30487..31002)
                     /locus_tag="PLW22_03525"
     CDS             complement(30487..31002)
                     /locus_tag="PLW22_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOU73807.1"
                     /translation="MKNIIKYFLPILSLLFIGFSYLYDNRAGEQIALNFWMFFKEMIL
                     FLPLMFILIGLFDVWVPREKIEKHIGEDSGWKGTLLVILLASLQAGPLYGAFPFAYLL
                     WIKGCNIRNIFIYLGAYSTIKIPMITFEIGFLGLKFSLLRTLITLPIFILIGFAMEKY
                     LKGKKFEVKQP"
     gene            complement(30999..31469)
                     /locus_tag="PLW22_03530"
     CDS             complement(30999..31469)
                     /locus_tag="PLW22_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOU73808.1"
                     /translation="MIGLTIVVSVLLVVSLLFDTQKTFKGVTRGVKMFLKLLPTLVTM
                     LALVSIMFFIIPNDTLVSLIGKDSGIKGYLIAAVIGSVSLIPGFIAFPLCKILIVNGV
                     EYSTVAIFITTLTMVGILTLPVEAKFFGWKVSILRNVLSFVAAIAIGITMIFLL"
BASE COUNT         8909 a         6504 c         6649 g         9729 t
ORIGIN      
        1 ttcgtaaagc cttgttcgac ttattcctag tagtgttgta aactcatatg tgcccatgct
       61 cgtcgaagtt acgctactct aaacttcgcc gagcgagggt tcgttccttt ttattttttt
      121 acagcagtga cttaacgtgg cattttcagt ttttatttta tcgatttacg ctcttatatg
      181 ataaatatga aaccaatacg cctaatgata atgccaataa agaccacgat aactcgttaa
      241 tatcaaaatt ccgtcttaac agaatactaa tgactatgcc aattgcaaca aaaactacag
      301 aggagagggc tctctttgcg ttaaacagcg acacgcctaa gtaaattaac atagtaatga
      361 agaaaaatat tacggagtaa aaatttcttt cgtaaaaata tatttgccaa aatattattg
      421 ctactattag caggcatatg atgataattt gaagtgattt atttttcatt tacaatgtgg
      481 gttttggttt ataaaatcag ataatttttc aaaaataatg aaaaattatc tgaaatgaaa
      541 aaaattacat aattgaactt cgaccactta tcgtttctaa atatgcacct ccatatctgg
      601 tttgatcttc atttggtaca ttgttatcta ttgaagataa tatataacca agccagtatg
      661 ataaccatgt cccaccatta atgtttaaca tatctgtagc attaagtggt tccaaatact
      721 gcatttctct tgctttaatt ttttgcaatg tggggttctt ttgtgtgttc atacctattt
      781 attttttatt ttgtttagca tcattccatc cttcccaaaa atctgcaggt gcgttcctgt
      841 ccagaagttc gcttacaata actcctgcta ataaccacaa caaagggtta cccccttcaa
      901 tctcaacaat ttctttggca tttaattgtt gtacgccaaa tttgttcaaa gatagttgtt
      961 ccattttaat ttagttttaa aaggttagta attttgccat ccaaatttgg ttgtgttttt
     1021 gtaacgttac agttgcaaag ttaggtgcgt tccgtccgga aaacaggaac ggaagtgaag
     1081 cgcccccatt tttttcaact aattcttttc ggctaagggg tttcatggtg catttcaacg
     1141 atatatcaaa agagtactca tagaaaaaag tttcacgtga cttagagtac acaattggag
     1201 ctcctcgcga ttttaactca tcaataactt cgtaaagcct tgttcgactt attcctagtt
     1261 ttgcagcaaa ctcatcagga gtgccggttc gctgttcctt aattaagcag tgcatttttt
     1321 cgatacgcac taagtactca aaaaatttca taactttttt aattttcaat tatatgtttt
     1381 tcaactgttt gatgttgcca catgcggcaa aactgcccat tactatgtat tagttcctta
     1441 aatactcctt gctctacgac cttgccattg tcaagaacta taatattatc ggccatagca
     1501 atggttgata gcctatgggc aataacaatt acggttttcc ctttttgcct catcaactca
     1561 acggtatgct gcacaaaact ttcggcaatg gagtccagcg atgaggttgc ttcgtcgagt
     1621 attagtatct caggattttt atatagtgca cgggcaatgg caatgcgctg cttttgcccg
     1681 cccgacaatg atgtaccgtg ctcgccaatg taggtattaa acctattggg taggctctct
     1741 ataaactcaa tcatacctag ctgggtgcaa atctcaataa tcctctgaat gtttgggtta
     1801 aactccccca ctgcaatatt ctcaactatt gttcctgcaa ataagtcgat attttgcggt
     1861 acagccacca ctgcatcgcg taacgattcg ttggtaaagt accgtaaatc aaactttcct
     1921 atgtaaatat ttccgctttg taatggatat atattctgca acagcgatgt tagggtagtt
     1981 ttccctgaac cactctcgcc aactacagcg gtaacttttc cctttggtat atgcaggttt
     2041 aacgccttaa aaacctccac ccttgttccg tagcggaaat caacattttt aaaggtaata
     2101 tcgccaacgt gctcagcggt gaattctatt ttgttttttt catcctccac ttctaagtca
     2161 aaaatttcga acaacctatc cgatgcaatt ttagcatctt ggtaagtttt attaaaacca
     2221 ataatactgg ttactggaga agtaagatag cctattaagg agtaaaatga aagaagttcg
     2281 ccaggggtaa ttgtgctttc tagaacaaaa ccagcaccaa cccaaagcag aacaatggta
     2341 attacctgtg atattaaacc tgaaacattg ttggaatata ttgagttaat tccggattta
     2401 tataatggtt ttagcaaatt aacaaatcgc atttcggttt ttaagttgga gtaaccttca
     2461 agcccaaacc tctttatagt tgatatagaa tttagggatt caaccaagtg cgattctaac
     2521 tctgctgaat tttccataat gctcctttgt accttacggt ttagcctatt aattatcata
     2581 taggtaatga tatatagcgg cattaccaaa agcataatta cagccaactt ccagtaaaat
     2641 gtgaacatca gcataaacga tgcaattaga attaaaacgt ttacaattag attaacggtt
     2701 acctcgttca caaataaccg aattttcact gcatcattaa ttcttgatat tatttcgcca
     2761 actctcattg tgtcgaaaaa ggactgaggc agccttagca aatgctggta gtatcccaga
     2821 attagcgtag catctatcct ctgcccaatt tttaaaacaa aaaccgactg gaatatgttt
     2881 agcaagaacc ttaccccaat tattattagc attgcaatcc ctaaaagatt cagtaaattt
     2941 gcattgttac ttggaatagc ataatcaact attttcccca cataaattga agttgataag
     3001 cccaaaaccg catatagaac tgctccaata attgcttggg ttaatacgcc cttgtaaggc
     3061 tttataagat tccagaatct aaaagatgat gaaacttttt cggatttggg tttaaatgtt
     3121 tcacctggca caagaaggat taatacacct gtccatgact ttttgaattc ttcgtgcggt
     3181 attttatgga acctaccatc gccgggatcc attacttcaa tgtatttttt ggttgtttta
     3241 gttattacaa caaaatgttg tagaacttct ttaacaacca catgtgcgat tgaaggtttt
     3301 ggtatcttga aaaggctatc gaaatcgcct ctaacgcctt ttgcatggaa acccattttt
     3361 tgagccgcct ctatcattcc taaaacattg gtgccttttc tatcggtacc agcgaactga
     3421 cgaatttttg ctataggtaa attcaactta taatgcgctg caactgaagc caaacaggct
     3481 gcaccacaat cagtaatatc tctttgtttt actctcattt ccgctatttc atttcagtgt
     3541 atggattaaa ccatctatca actttatcaa aaagtaaatc atatacacta cgctctgtta
     3601 ctattattct tgatgatatt gtcataccct tttttatgta tcctttaaaa ccgttttgaa
     3661 gtgttaaata gttttttgat aatttgcact taacaataaa gtacatatta ttctgcccat
     3721 cacttactat atcatcagaa acctccttta tggttgcagg cagcaaaccc cattggttat
     3781 aattgaaagc atccacctgt attttcacat cctgattaac ccgtataaaa ccgatgtcgc
     3841 tgggttgcac atagcaaact gcaattaact cgcaatcggg cgacaactct gcaattattt
     3901 gattggcata aataatacta cctacttgca actcattaca ttgttgaata gttccgctca
     3961 aagtcgccgt aataaaatgc ttgctaagtt cattatatgc tgtttctatt tcggattcaa
     4021 tctttggaat ttggttggta atctcggcta ggcttgcttg ccagctggct aactgctggc
     4081 ttataaattg atttcgaagt aaaatctcat tgtccaagtt gtacttactt gtttcataat
     4141 cgacctcggc aattattttt tgcttgtata aaagggtgtt tcggctatgt tcttttttca
     4201 acttttcaat ccgattactt tgtgttgata tttgacgctg aaaactactt acctcaagca
     4261 tatatttgga agttaccaaa ctctttggat tgaacttttg tagtaaaaaa ttatgctcaa
     4321 taagtaaacc caaatcgttc tgatatgatt ttaactcacc aagattaaac tctaaagttt
     4381 taattttaga actaatgggt atggtgtcta ttactaggat tgtatcgcct gcacttaccc
     4441 ttaaattatt ttttagttta gtgtaaatga ccttaccccc atatggtaag acaatattct
     4501 gcttttcaat gcttggctta atcaatcctg atgttcgaat agtaacgtct accaaaataa
     4561 atggcataga tgctagagtt cctattatta tcattatgat taaccaatag atttttttgc
     4621 ttcttttcga gatggaatgt aaataaccct ctacagaatt ttgtataaca aatccaggaa
     4681 aaactctttt taattctgtc atatattcaa tgttttttaa attcctatca aggtttttgc
     4741 ttaaaaagag aatacgtatc aaagatttct agtaaataga tagatttaca tgacaatatt
     4801 tgaagtgatg ttcttttatt attcttttgt ttatcaatat ctggcaaaca ttatactata
     4861 atgcaaatca tttacattat tattgtagta ttgtgttgca aatttacccc cccccccgca
     4921 tcaatgcgcc aaatttatta atatgtttta taacatgatt cagaatgtta aaatatgtta
     4981 atgataaata aagttgatga tttaccatat aaactttaaa acaatttttt ttattcacaa
     5041 aaagttgatt ttctttccga tgggttgtat ctttgaaacc gatctgggac aaattgttta
     5101 actaaaattt taagctatgg tgattcgaac agctaagttt gtggctagtt atcctagtgt
     5161 gcaaaagtgc ccaaaaaccg atttgcctga gtacgcattt atcggtcgtt ccaatgtggg
     5221 taaatcgtcg ctcattaaca tgctcacctg caccaaggcc ttggctaaag tttcgcaaac
     5281 accgggcaaa acacagctca tcaactatta cctcataaac gactcatggc acctggtcga
     5341 tttgcctggt tatggctatg ctaaagtatc gaagggtgag cgcgaaaagt tctcggatat
     5401 agtgctcaac tacatactca agcgggagaa tatgcacttg ctttttgttc ttatcgattc
     5461 ccgccttgaa cctcaggata tcgacatcca gtttattaac tggcttggag ttaaccaggt
     5521 tccatttgcc cttgttttca ccaaaaccga taaggtttcc agcatggtgc ttgagcagaa
     5581 cattgagctg cacaaaaaag tgctacagga atcgtgggag gagctacctc caatttttac
     5641 aacatcgtgt gtaacccgca caggacgtga ggatgtgctt aattacattg accaggtttt
     5701 tcgggaagca aaaataactc attgccaggt atagaacatg agctataaaa tttgtaaatt
     5761 tgcgagcatt aacggaaaaa aatcctaaaa tgcacagcaa acaccaaata aagttcttca
     5821 ccggacgtag ctcgcgttat cttgccgaaa gaatagctca aagttttgga attccactcg
     5881 gaaaatcaac cctaaccgtt tttagcgatg gtgagtttca accctcattc gatgagtcgg
     5941 ttaggggatg tactgttttt atcattcagt caacctttcc accggttgat aatctttttg
     6001 agttgctact gatgattgat gctgctcata gagcctcagc gtataaggtg gtggctgtga
     6061 tgccttactt tggttgggca aggcaggatc gtaaggataa accccgtgtt gccattggcg
     6121 caaagttggt tgccaatatg ctcgttgcag caggtgccga tcgtgtaatg accatggacc
     6181 tacatgccga ccagattcag ggctttttcg atgtgccggt tgatcatctt tacgcgtcat
     6241 caatatttgt cccctatatc aagagtctta atttggaaaa cattgcgatt gcagctcccg
     6301 atatgggtgg agcaaagcga gccaacgcat actctcgatt cctaaactca tcgctggctg
     6361 tttgccacaa gaaccgcgag aaagcaaacc aggtaggtga gatgactgtt attggcgata
     6421 ttgagggtaa gcatgtaatt attctggacg atatggtgga taccgccggt acccttacca
     6481 tggctgccga tttgatgatg ggtaaagggg cggcatcggt acgagctttt gctacgcatc
     6541 ccgttctttc aggctctgcc tatgagcgca ttcgcgactc aaagcttacc gaactggttg
     6601 ttaccgatac catacccctg cgtaccgata aggatacttc caaaattaag gtgctttcgg
     6661 ttgctgagct ttttgccgac gttatcaata aggtttacaa ctaccagtca atcagcgaaa
     6721 agttcatcat ttagttttaa atattttttt tactatcttt gcagcccatt tccctcatga
     6781 tagggtacga tggggggctg gttatgcaat tgagggacag ccctgagtag tacctaagta
     6841 ataacaaaaa ttatcaagag taaatgaaaa caattgaact ttcagcagcc cctagaaccg
     6901 aaaccggtaa aaaagctgct aagcacagcc gcaagtcgga gcttgtaccc gccgttattt
     6961 acggtgctgg tgagaacatt atgatttcgt taggcgagaa ggaactcaag aaggtgattt
     7021 acacacctat tgtttacctt gtaaagctta acgttgatgg taaaatgcac gatgctatta
     7081 ttaaagaggt tcagtaccat cctgttaccg ataagattct acacgttgac ttccttaagg
     7141 tttcggagaa caaacctata accattggtt taccagttaa cctggttgga caggctgagg
     7201 gtgttaaggc tggtggaaaa cttttgcagg tagtgcgcaa aattagggta aaaggtttag
     7261 ctaaggacct gcccgatgcc atctccattg atgttaccca tcttggaatt ggcaagagca
     7321 ttatggtagc tgaactaagt tttgataagt ttactgtggt tgagcctaag agcatggtaa
     7381 ttgctaccat taaatcgacc cgtgctgctc gcgaagctca gcaggaacaa gctaaataag
     7441 tagttcagaa ttgaagtatt taattaccgg gttagggaat attggaagtg agtattccaa
     7501 cacccgacat aatgtaggat ttatggtgtt ggacgctctt gctaaggcgt ccaacattac
     7561 ttttgccgat gcccgctacg gctttgttgc tcagctcaag cacaaagggc ggacatttgt
     7621 actccttaaa ccctcaacct acatgaacct tagcggtaaa gcggtaaact actggttgca
     7681 aaaggaaaat atcccattgg aaaacctctt ggtggtagtt gacgatattg ccttacccct
     7741 tggcgcaata aggttaagac ctaaaggtag cgacggtggc cataacgggt tgaggaatat
     7801 aaacgacgtg ctgggcacac aggattatgc ccgtcttcgt tttggaattg gtaatggatt
     7861 tagtcgtggc cgccaggtcg attacgtttt gggtgagtgg accgatgagg agctgaaggt
     7921 actgcccgaa cggctaacgt tggccggcga ggctgtgctt gcctttggca caattggtat
     7981 tgaacgctcc atgaaccttt acaatagccg atagaatgcc gaccatgagg gttgataagt
     8041 ggttgtggtt tgtgagggta tttaaaacaa gaacccttgc taccgagtat tgccgcaaag
     8101 gtcgggtaac ccgtaatggg attgaggtaa aaccctcgca ggatattaat gtaggtgata
     8161 cattaaaggt tcgaaaaccc ccggtgacct atacattcag ggtgaaagac ctacccaaaa
     8221 accggcttgg cgccaagttg gttgaatcgt atatggaaaa cttaactcct cccgaggagt
     8281 tacagaaact tgacccatcg ttcatggcat tcaacataca ccgcgaacgc ggaacaggcc
     8341 gccccacaaa aaaggaacgt cgcgagattg atgaactatt cgattccggt ttcgggggct
     8401 ttgattggga tagctttgag cctgatgccg acgatgaatc caatgagtaa ggagtctttt
     8461 gattcaagct attactcgct gctgtattgt tacatttcct ttaagccatc aaccctcttt
     8521 tttgaatacc gatgtgattt cagcgataaa tagcctatgg aaatcgcttt tagggtagtg
     8581 cttactgatg atttccttat cgagaaagtt ctcgggtttg atatcatcga tataaagttt
     8641 tcggcattca atcaccagtc gggcctcctg aaaggctatg gccttaagcg attcggcaat
     8701 ggttaatccc gctgccttag ttttatcgat atcgcgaccc gatttgctgc cacatagctc
     8761 cagtgccgaa cgccatttct cttcaaaaaa gttgagggta aagtaatcgt tttgctccac
     8821 aaactgcaat gtgtagcgct gagggcgtat gaatacaaat gccaccggtt tattccatag
     8881 tattccaaat cctccccacg aggctgtcat gctgttatgt ttatccttgc tgccagcaag
     8941 tattagcatc cattgattgt cgagcaggtc gaaggtgttt tcatttaact cgaaaggcga
     9001 aatgaacttg taattttttg gttccatatc aatttttgtt taattcagtc aataggaatt
     9061 cccaggtagt ttcgaaccag caagcctata ccaaaggtta gcgctgaaac tcctaagcta
     9121 ataacaacca tttcaaggaa cctatgctta aagttgaggt ttttagctac tgcaatgtag
     9181 aagttgaagg cgaaaatgat ggctattgca agcagaagtg ttgttgctag ggctagcatg
     9241 tagcttgtga gcagtagaaa agggctaatc agtattgcaa cggtaaatat ataagccaaa
     9301 ccagtataaa ttgccgattt acccgcgtgt cggtcatcat tttcagtttt aacggaaagg
     9361 tactgcgatg ctgccattga gagcgaagcg gcaatacccg taataagtcc ggcaactgca
     9421 atgagtcggg tgttttgcat ggcaaggcta aagccagcta aagcgccggt tagttcaacc
     9481 agcgcatcgt ttagcccaag aaccattgag ccagcataat caagtctctc ttcctgaatt
     9541 aatgcaatta gctggtcttc atgttcctct tcggattttt ttacggcttc ggcttcgggc
     9601 atgtagggta ttactcgttc gtaaacatcc tgaatttttg cctcctgtcg ctccataagc
     9661 cttaaggcga atgtaagccc aagtattttc ccgcaaatgg tgaaaaacca tactacagtt
     9721 ttttgagggg caacatcaac ccccgatatc tgtttgaaaa tattgtagtg cttaagttcc
     9781 tcaccagcaa tttttgctaa aacagtttta ttgtttttgt cgtttgttga tttagccaga
     9841 ttcttataaa cctggtagcc tgtaatttcc tctcgctgga atagttttgc atgctttaca
     9901 agttcttctc tagtcattgg ttagtattta ctttttgttg aagtattgtt ttagttttgc
     9961 cacagtgctg gcaatggttt cggcattggt ttgctgaacg gtattttccg gaagcaatgc
    10021 cgaaacaaat gaaagtccgg tatggttggt attgtaccgg gctacaattt cgctgccgct
    10081 catatggaac tggtttacac cattctgtgc tagctctaag atattgctgt gattaatccc
    10141 tcctccggca attagggcta ttttgttacc tccctcggca attaactgcc ttatcaggtc
    10201 taaaccatca acagccttgt ttttttgccc tgacgttagt aggaaattgc atccacattc
    10261 gataatttca tttagtgctt tgaaaggttc gggcattatg tcgaaggcgc ggtgaaaggt
    10321 aaaggtcatt ggtttggctg cccaaaccag ttctttagtt ctttccttat caagagtacc
    10381 attcgcccgg agtataccgc taacaacccc ttttacccca agttttttgc atgcctcaat
    10441 atcgcggagc atttgacgga attccatatc ggagtacaca aagtcgccgg ggcggggacg
    10501 aataagaacg tgaaccggaa tatttagctg gttaagggtt tcggcaatgc atccaaacga
    10561 tggggtggta cccccaacgt aaaggttctc acagagttct attcgatttg ctccgccttt
    10621 ttgagcgtta atggcactct ctacagagtt ggcgcaaacc tcaagggtaa acatcgttta
    10681 ctgctttagt gctgaaagta gctgttcgcc aaggtttatg ctataacccg atgaatgata
    10741 tactatctct ccgttgttgt tcacaacaag aacaacaggg tattccagct gtttgccaac
    10801 tgcctgccct accttagcct caaattgacc atttatatcg ttcccgaggg taatggtatt
    10861 ggggtagggc tttccatcga aaatggatgt caaatcggtt tttgccgatt tgccacttgc
    10921 cagaataagg gcaatactat ttcctatgcc actaaactcg ctacataggc tgctgatatc
    10981 gcgaagcaaa tgctttgtag gttcagcaag cgggtcgata atgacaacta ttgctggttt
    11041 atccttggta atctgttcaa ttgtttgggg gttctggata aaaggtatgg tgatatcggt
    11101 ggtaatggtt cccaaaacca ttttctcggt ttgggttgat gggatggtta gtgtaaattg
    11161 tatctttttg ccactttcaa ccctgacgta gtaaacattg cagttaactg ctcccgattt
    11221 atgcctgtta ccggttacta tccggtaata accctctggg agttcaagtg ctgcagggaa
    11281 tttgttgaat aaaggacttt cctcataatc aagggttgag tactttccct tttcgaaccg
    11341 tgcaatggtg aagtgtgtgt agtactttgg ctgttcaaca gccgagtttt tatcgaggtt
    11401 gataattagc atcccggttg gtgttgtggc ctgtgtctct tttacatcgg cgatatttac
    11461 atcaatccac ttccctttca tgaagtactg aggttttttt gatgctggct ccaatcgggc
    11521 agctattcca aaactgcgac atgctgctac aaagaaaata gccatggaat attcatcggc
    11581 gcggcgaatc cggtaaacgc tttcagggct taacggcaca cggtagtaat tgctttcggt
    11641 gtcaagtgtt acaaactctt taatccattg ggcaatgttt tgaggattgt ttcggaaaaa
    11701 accaatctcc tcaaaggtaa aggcatcctg caagaaactg cgccagggtg taataaattc
    11761 tcggccaatc cggggcgaaa gaatgtagct gtcaaactct ttaaccatgc ggctttcaag
    11821 cagggcaggg aaagaatcga ttcgtgaaag gtggtcgaaa agtacatcgg tggtaatatc
    11881 gcgtaaatcc ttttcccgaa gagttgaaag caaggccact ccaacagaga tgtctttagt
    11941 gtccaagttc tttatgtagc tgtaaatctc attccagttg ccgcggcttt ttgaaagcca
    12001 tttccatgtt tcatcaatgc tgataccctt ttccatggct aaattggctg ctgaagccga
    12061 gtcaataaag gtgcttatga aattactacg aatttcattc ccctgtttaa ccctgcggtt
    12121 gtgttcatcg gctgtacttt catcgtatgg tggaatagtt tgttcgtcgg gtggggtaag
    12181 ggttaaggaa aggttagtaa aagggatttc ctttttgagt gttaggtaaa tatcgttttg
    12241 gtttctgtcg gccttggaaa aggcataggt gttatccttt tgtgcccaaa ccatcaagtc
    12301 gccaatccct gtggttaaag tacacaagcc atttgcattg gtttgcaggg tggctatggg
    12361 gtaaaactcg gcgtagttgt aaagctggta ttcaaccttt gcgccctcaa ccgggtttcc
    12421 ggtgctatcg gttacggtaa cggtgagttt acgggtaggg gcatagttag gcataaggtt
    12481 aatctcagtg taatagttgg tttgtttaag ttgttcctca gggccattgt atttgccaaa
    12541 aacaaaagta cgtgtcatca tggctcgttt ggcaggtcct gcaaaccatc cacggtcgag
    12601 ttcaggctca ggttcgcatg cgcctaagaa gtgccattta ccatcgaccc atacctctac
    12661 ccaggcgtga ttgtcgtcgg tgtgagccca gcggggtgtg tagacctgac gtgctggtat
    12721 gcctactgaa cgataggctg ctactgcaaa tgtcgattcc tcgccacaac ggccaatggc
    12781 tgaacgaact gatgttagcg gcccacatgt gcgctcatcg gtcgattggt aggtaacctt
    12841 ctcgtggcac caataattaa cctcaagcgc agcctggtat gtattgaggc ccagaaggcg
    12901 ctcctttagt tcctcgaaga aaatctcgcg ggcgttatcg gtgtactcgt tgttaaccct
    12961 gtaaggtagg acaaagtgaa gaaatatatc agctggcact ttccttgccc acttaaattc
    13021 tttccgcgct tttagcgcta gctgaacgtt cttcagaaca tactctggtg agtgcattgc
    13081 taaatcgctt agtggcatgt atgcgtaaag aaactcaaac ccttcgcgct ctttagtggt
    13141 aagttctgag ttgattattt tgtaaatgga tgaatcgtag gcagcaatta gcttgaccct
    13201 ttcctcgaat tgggcatgaa cctctttgcg tttatcgctg cctttgatga ggtggcggtt
    13261 aagaatggta tcgcaggagc taaggattat aagcagcaat gcgctgatag ttacaatctt
    13321 cttcatgttg taagctgttt gtaatcaaaa aataaatgta agtaattttt cgattcaaac
    13381 ctaaaggtgt tgggtaaatt gtgattttat ggtaaaataa aagaaaactg cctcaaaaag
    13441 atttttttgt tcaggccatg tattgaacgc aatgctgaga taggttcaaa accgttgccc
    13501 tttatctttt tgaggcagta ttgtgtggtt aatgtgttgt tttactgcgc tttgagcgtt
    13561 tccctgattt ttgtttcaat ctcgtttgat agttctggat tatcgagcag gagctggcga
    13621 acagcatcgc ggccttgacc aagtttagtt tcgccatagc tgaaccatga accgcttttc
    13681 ttgatgatat tcagttcaac gccaaggtca atgatttcac ctgttttgga tattccctca
    13741 ccaaaaataa tatcgaactc ggctttgcgg aatgggggcg caaccttgtt cttaacaatt
    13801 ttaaccctaa cgcggttacc ggtcgattcc tctccatcct taagctggtt cagcttacgg
    13861 atatcaacac gaaccgaagc gtagaacttg agggcattcc caccggtggt ggtttcaggg
    13921 ttgccaaaca ttacgccaat tttgtcgcgt agctggttga tgaaaataca gcaggtgttg
    13981 gttttgctga tggtggcagt gagcttacgc aacgcttgcg acatcagtcg ggcttgaagg
    14041 cccatcttgc tatcgcccat atcgccttca atttcagctt taggggtaag ggcagccacc
    14101 gagtcgatga caatgatgtc aattgagcct gagcggataa ggttatcggc aatttcaagg
    14161 gcttgctcac cgttatcggg ctgggcaaaa agtagattct ccacatcaac gccaagcttc
    14221 tcggcatagg tgcggtcaaa agcatgttcc gcatcgatga tggcggcaag gccgccttgc
    14281 ttttgagctt cggcaatggc gtggatggca agagtggtct tacccgacga ttcggggccg
    14341 tagatttcaa ccactcgccc acggggatat ccaccaaccc caagggctac atcgagcgct
    14401 attgaccctg atgagatgac tggcacttcc gatacggctt tatcgccaag cctcattatt
    14461 gaacccttac caaaatcctt ctcaatttta tcgattgtcg attgaagggc ctttagcttt
    14521 tccttgttaa tttccttgcg ttccttgttt tcagttgctt ccatttactt atttatttcg
    14581 gatagtattt gttttacgtg ttccttggta tcaaccttat taaaaacatg tgtgatagtg
    14641 ccattttcat ttatcacata tgttgtgcgg agaataccaa agtatgcttt accgtacatc
    14701 tttttctcac cccatgctcc atacttttcg gcaatcgcat ggtctacatc ggctattaag
    14761 cggaaaggca ggttgtattt atgaataaaa ttttgatgcg atttctcgga atcggggcta
    14821 acgccaacca cttccatgcc taagctctgt aaatcatcat aaccatcgcg taaactgcaa
    14881 gcttcagcgg tacaaccgct ggtgttgtcc ttggggtaaa agtaaaggat caactttttc
    14941 cccttaaaat ccgatagttt aatggtttta ccatcctgat ccttcccaat gaattcagga
    15001 gccaaatcgc ctggttttaa gattgttgtc gccatagtaa tctgtttgta aagttaattt
    15061 tttgtttgtt aaatggaaat gccttaggca tttatttatt aacaagctgc aaggtgtttg
    15121 gttttaaaaa atgaaagtta gttaacctca acaaaaaaag ttcatatcct tttgtaaact
    15181 atgcgataaa tgctcttttt aatcctgagc aaatacttta tctttttcaa tgtcattata
    15241 aagatattca gttaccggca aacgggaaaa tccgctcatt ggggtaaaaa ctattcagtg
    15301 caggcttgaa ttgatggttt taaatatggc tagttctata atagcactaa aaatcgggta
    15361 cataaaagaa gtagcctaat ccaatggtga ataggaaatt gccaacaatt gcatgttcta
    15421 atgatactac tagtattgag cgatgtttaa agtagctata tgcccaaaca aaaccaatta
    15481 tggtagcacc agcaacagct atccagtttc taaaaattat atgaagtaat ccaaaaagaa
    15541 tcccatttaa tgtgataaca attttatcat ttttgaaaag gggtgtgtat cggtggaaga
    15601 aaaacccacg gtatataact tcctgcgtaa aggctgacca aatggggtaa aaaaacatga
    15661 ttagtagcca aagctctgtc ctatgtaatg gtaagtaaaa gagttgctca ctaggaagga
    15721 agataaaact aagtaaactt attgccagta gtgaaatgag aatacgcaag ctaagcgttc
    15781 tccaacttct gtatccatta aatccaaacg attttttact aaacgatttg gtggtgagta
    15841 aataaaatag tgcaattaaa aacactacta aaagcggaat gcttttgggt aatttcaggt
    15901 tgttgaagta gaaagctaca ggtagcccaa caaaaagggc agccagttca atccaccgaa
    15961 caatttttgg aatcggtgcc attgctggaa atggttattt gggtaattca acaattactg
    16021 ttgtcccttt gtttggttcg gagataaatt taaggttgcc ttttagcttg ttggttaact
    16081 ctttgcaggt aaacagacct attccagtgc ctttttcgtt atcggttcct ctgcttgatt
    16141 gataattctt aaaaagattt ttaagctgtt cggctgtcat accaatacca ttatcaacaa
    16201 ttttgatgga aaggatggtg ggctcattgc taacctggat ttttatttca ccttccgggt
    16261 aagtgaactt tatagcgttt gaaaccagat tacgaatgat gacagccagc atatcggggt
    16321 cggtgtttac cataagttca ctgggaactt cttctgttag cgtgattcgt tttttttgcc
    16381 aaatggggcg cagaacgttt acctgctttt gtatcaggga gtcaattggg atttccctgt
    16441 atttgggctc aatggcattg gagtttagct ttgcccagtt tagaagattc tcggttaaat
    16501 cataaaggtt gtgggcaagg tcgtttagca gctgaaggcg aagctgaaaa tactccttgt
    16561 tttcaaaatc gtcctcaaag ttcatcagca taaggtcagt tattcccaga aaggcgttga
    16621 atggactttt aaggtcgtgc gacacaatag agaaaagctt tgaaagcgat tggttcattt
    16681 cctttagctg ctggttctgc ttttccagct cctgttgttt gataataagc tgattcttaa
    16741 gttccctgtt ttgcttgagt acagccgata ggggtacact ttcgccctcc tgttgcatct
    16801 ctgtgggctg cgaccaatgc ccgtgaataa ttttccactc acctttttct ttacgtagaa
    16861 tggcagttgt tctgttgttt gtgtattcaa actcgccggt tgggagcagg aacttcatgt
    16921 caactaaacc cattacatag gccgaatcgg ctgtaatggg acataccttt aaggatttaa
    16981 actgataggc aaggggttcg ggcgcctggg cgaattcccg tcggaatagt tcagtggagt
    17041 gctgatatgt tacgctgaac tcatctactc cagttccaat catgttaaaa tcaggagaaa
    17101 acgcattaag cgtatcttcg agcttgcgtt gaacataatc ttcaaaatac ttaaggtacg
    17161 cctgcttaac ttcgttctcc attttattca aattgaatct caaatttaaa aatcaaaagt
    17221 tgtaatatgc aaactctttc tgttcttttt tgtaaagttt tgatttttag aaaaaagttg
    17281 tagttttgta tgaacattca ggatatgatt ccacttaaaa aaatattgct gtcagtaaag
    17341 catacaggtt tcgaacaaac aatactttca gctaactcta ttgataacct gccagttttg
    17401 gcaggttttt ttttgcccta caggaaaggg ctgcaaacgt tcagtttaaa gttgatttat
    17461 cgaacttcaa actataaatc tcttaaatta ttccagaatg aatcaacaac aaaaagcaaa
    17521 gcttgtgctg gaggatggct cagtttatac tggttactcg tttgggtatg ccggtaacac
    17581 atcgggtgag gttgttttta acacggcaat gaatggatat cccgagagtt taactgatcc
    17641 gtcgtacttc ggacaaattc ttgtggccac tttccccttg gttggcaact atggggtacc
    17701 cgaacctaat cctttgggag gaattgaaca gaattgggaa tcgggcagga ttcacattaa
    17761 gggcttagtg gtgtgcgagt acagccaaaa ttatagtcac tggaatgcga acaaaagcct
    17821 agggcaatgg ctaaatgaaa acaagattcc ggcaataagt ggtattgata cccgcaagct
    17881 taccaaacaa ttgcgcgaaa agggttgtat gttgggaaaa attattgttg atggcgatac
    17941 ggattggtac gatcccaatg ccgataaccc ggttgcacat gtatcggtag caaaaccaat
    18001 tacctatggg aatggaaaga aaacaattgt tctggttgat tgcggagtaa aaaacaacat
    18061 cctgcgttgc cttttaagac gcgatgtaca ggttattcgc gtgccttaca actataactt
    18121 tttgagccag aaattcgatg gtgttctcat ctccaatggt ccgggcgacc ccaaggtgaa
    18181 cattgaaacc attgataata ttcgtcggct aattgaaatg gataaaccgg taatgggaat
    18241 ttgcctgggt aatcagcttt tggctttggc ctcaggggca aatacctata agctacccta
    18301 tggccatagg gggcataatc agcctgtgag gctaaaagga actgaccgct gctttgttac
    18361 ctcgcaaaac catggttttg caattgagcc atcgaccctt tccgatgaat gggaggtgat
    18421 gtttgaaaac ctgaacgatg gaacctgcga gggtataagg cataaagcca agcctttttt
    18481 tgcagttcag tttcatccgg aggcttcggg aggaccaaac gataccgagt tcctgttcga
    18541 tgagtttatt aacctaattg acaacaagta aaatagattt atatgccaca tttacgcaaa
    18601 tacagcaaag ttttagtgct tggttcggga gcgcttaaga ttggcgaggc cggcgaattc
    18661 gactacagcg gttcacaggc gctaaaggca ctaaaggagg agggaatagc aacagttctg
    18721 gtaaatccca acattgccac ggttcaaacc agtcaggatg ttgccgatac catctacttt
    18781 gaaccagtaa aacccgaaac ggttcgacaa atcattgaga aggaacaacc cgatggaata
    18841 ttcctctctt ttggtggaca aaccgcacta aactgcgggg tggaacttta ccagaatggc
    18901 acactccggg agttcaatgt tgaagtgctt ggtacgccag ttagcactat tatcaacact
    18961 gaggatcggg aactctttgt taaagagtta aaccaaatcg gggtgagcac tccacgaagc
    19021 attgccgtga atactgtggc tgaagcggtt gatgccgctc atcaaattgg ctacccggtt
    19081 attgttcgtg ctgcctttac tctgggaggg cagggtagcg gattctgctc aaacccacaa
    19141 gagttaaagg ctttggcaac caatgcgctc acttatgcac cacaaatact ggttgaagaa
    19201 tcgctaaagg gctggaaaga ggttgagtac gaggtggtac gcgataggtt cgataattgc
    19261 ataacagttt gcaatatgga aaacttcgat ccattaggca ttcatactgg cgaaagcata
    19321 gtagttgcac cttcgcaaac actatcaaac tccgagtacc ataaattacg ggcgctctca
    19381 ataaaaatag ttcgtcacct gggaatagta ggggagtgta atgttcagtt tgcactcgat
    19441 cctagtagtg agaattatag agtgattgag gtaaacgctc gcttatcgcg ctcatcggct
    19501 ttggcatcca aagctaccgg ttacccacta gcatttgtag ctgctaagtt aggtttaggt
    19561 tatggcctgt ttgagctaaa aaactccgtt acccacaaaa caacggcatt ctttgagcct
    19621 gccctcgact atgttgtggt aaaaataccc cgatgggatt tggctaagtt taagggggca
    19681 tcgcgccaaa ttgggtcggc tatgaagagc gtgggtgagg ttatggccat aggccgttca
    19741 tttgaggaag ccattcaaaa aggccttagg atgatagggc agggaatgca tgggcttgta
    19801 gcaaatcagc attttaaccc gtcggacctt aaactgatgc ttgagaatcc tactgatcaa
    19861 agaatttttg ccattgccca ggcatttgag caaggatgga ctgcggagga tattaatgca
    19921 atcacaagaa ttgatagttg gtttctcaat aagcttcagg gtatagttga actttccagg
    19981 catcttgcag gttactcagc cattgagcaa gtaccatcgg aacttgttgt tcaagctaaa
    20041 cggaatggct tttccgattt ccagttggct cgtatcattt acaatccatc caacaataaa
    20101 attgagcagt accaacagca ggtacggaac tacaggaaaa gctgtgggat tgttccttgt
    20161 gtaaagcaga ttgacacatt ggcagccgaa ttcccatcgg aaacggcata cctttacatg
    20221 acctaccatg ggaatgagga cgatacatcc gtaaaaccta agcccaaagt tcttgttttg
    20281 ggttcagggg catatcgtat tggtagttct gttgaatttg attggtgcac cgttagtgca
    20341 gtaaaggagc tccgacgctt gggatttgag gcgattgtag ttaactacaa ccccgaaacc
    20401 gtaagtaccg attacgacgt atgcgataag ctttactttg acgagctttc ctttgagagg
    20461 gtgatggata tcgttgaggt tgagcgtccg ttaggtgtaa tagtatcggt tggtggacaa
    20521 atacccaata atctggcagg aaggctgagt aaagcagggg tgatggttct gggaacctca
    20581 cccgtttcaa ttgatatggc cgaagatcga caaaaattct cggctttgct cgataaactc
    20641 ggtatcgatc agccagagtg gcaggagtta accaccatgg aacaggtgca ggagttcgta
    20701 aatcgtgttg gttatcccgt tttaattcgt ccttcctacg tcctttcggg ttcggcaatg
    20761 aatgtggttt caaacgccga cgatttgaac gagtacttaa ctgttgcagc cgaagtgtcc
    20821 aaagcccatc cggtagtaat ctctgagttt atcgaagggg caaaggaaat tgaatacgat
    20881 gctgtggcta ataatggtcg gatatttgtt gatgccatat cggaacatat tgagtttgct
    20941 ggtgttcact cgggcgatgc taccctggta tttccgcccc agcgcctcta cctggaaacc
    21001 atccgccgta ttaggcgtgt tgctgcagct cttgcagccg aactaaaaat tacaggcccg
    21061 tttaacattc agtttttggc aaagaacaac caaattaagg taatagagtg caacctcagg
    21121 gcaagtagaa gttttccgtt tgtttcaaag gtgatgaatt ttgatatgat agccgttgcc
    21181 gttgatttta tgatgggtgt agcacctaaa accaatccac catcgatgct cgatatcgcc
    21241 catgtgggtg taaaagcctc acagttctca tttggaagga ttacctttgc cgacccagtg
    21301 ctgggtgttg agatggcctc aaccggtgag gttggctgca ttgggcaaag tgttaatgtg
    21361 gccttgctta aagcgatgtt gtcggtaggg catagaattc cacagggttc aatcttggtt
    21421 tcatctgggc ctttaaggtc aaaactctcc cttatcgatc ccattgcccg tttggccagg
    21481 cgtggttttg taatctacgc aacaagggga actgcatcgt tccttaacga ccatggcata
    21541 cctgccattg ccgtttcctg gcccgatgag ccagaaaatg ctcctaatag cctgaatctt
    21601 attcagaatc gactggttga tttggtaatc aacatcccta aaaatctctc gcgcagcgag
    21661 ctcgataacg attataccat tcgccgttca gctgttgatt acaacatccc gctaattacc
    21721 aatgctcgac ttgcagccgc ttttattgag gcgtttctga ataccgataa tatccaaatg
    21781 gaggtgaaaa gctataatga actagttgga agttaggata ggggcctact aaacttattt
    21841 tggttaactt tgtttactaa ggttcattta ataccgtgag atatggagta tgagatgtca
    21901 aatatactta tagcatttgg tttaacactt tttgctggtc tttcaaccgg tattggaagt
    21961 gctattgctt ttttttcaaa gcataccaat actaagtttc tgtcgcttgc gctagggttt
    22021 tcggctggcg taatgattta tgtgtcgttt attgaaattt tttccaaggc gcgtgagtcg
    22081 ctggtcgatg caattggctt gcatttaggt tcatggatta cggttgcctc cttttttgga
    22141 ggcatggcgg ttattgcgct gattgataag tttatacccg agcaggaaaa cccccacgag
    22201 gttatgctgg ttgaggatgc tgagagtggc cgaaagcgta aagggaaact gatgcgcatg
    22261 ggactcttta cagccttagc catcgccatc cataacttcc ccgagggctt ggccacgttt
    22321 accgcggctt tatcgaaccc taacctgggg attgccattg ctgtagccat tgcaatacac
    22381 aatattcctg aaggtattgc tgtttcggta ccaattttct atgctacagg cgataaacga
    22441 aaggcgttta ggctatcgtt cctctcgggc cttgctgaac ctattggtgc tatagttgga
    22501 tatttgctgc ttatgccgtt tattaatggt gttacatttg gtattctgtt tgcagcagtt
    22561 gcaggtataa tggtatttat ctctgttgat gagttactcc ctgctgcccg cgagtatggc
    22621 gagcaccacc tttccattta cggccttatt ggtggcatga ttgtcatgtc aatcagcctg
    22681 ttaatgttta tttagttgga atgaaataat aaacggctgg tgttttagcc aaccgttttt
    22741 gcattatagg tagttttagt acttgttagc acaatcgatg caaacctttt tatctccttt
    22801 aatgcgtccg tactccataa ccgtcatttc gccacactcc tcgcatacaa agctattaaa
    22861 actgtgtggg tgttctttaa ggtcatactg gaagacctct gaaatattga ttaacttttc
    22921 atcaggggcg ttcattacat tattaaccaa agggtcaacc accaaatcgg gaactttact
    22981 tgcgggtatt cccttttcgc ggtaatcctt aaagaatgat gtttgcttat tggcaagcat
    23041 tgcttctgct ttgggtgtaa cccttacagc gcggccggta gctttgtcaa taacagtaac
    23101 ggcccatttc cctttatggg ttttcttgat attgcctttg ccaaaggttg cgcccagtat
    23161 aacctgtaaa ccatcggcat agcaggtggc acaatggtca tcgccaaggt caacaagggc
    23221 tattagctgg ccatccttag cacgttccac acccagtgca ttcattgcag cagcacctac
    23281 cctaagcccc ataggcattg caggacattt atgtccatgg aatttttgac caaattccaa
    23341 ccaatcttta ggattaatca tagtgtaaaa atttaattgt taatgaatta gttgtttatt
    23401 atcagtaacc gaatactccg gttgaatagt tacatgattt atgccttgct ttgcaagcaa
    23461 aatttttact tgctcaagaa tcttctcgaa atccgatatt gtgatattgt tagttacatc
    23521 caaatgggcc tcgaacatga tttcgtgctc atcaatttgc cagatgtgca catggtgtaa
    23581 gttttttacc ccctcaagtg attcaacctg tttagtaatt tcattgatgt ttatattctc
    23641 aggggaaaat tgcataaaaa ttctcaagga agcttttatt attttccacg atgtaaaaag
    23701 taagtatatg gcaattagta tgctaaagag ggcatcaacc cgataccatt ggaggtattt
    23761 catagccagt ccaccaatca gaacggctac agaggtaagc atatcgctga aaagatgaag
    23821 gtatgccgac cgtatattga tgctattgcg tgaatcagct tttatgaaaa gaacgcttaa
    23881 gccattaacc aaaatactta ataacgatag ccatatcacc caaccggaat caattggaat
    23941 gggtttataa aggcgtttga cgctctccaa aagaatagca atagctaaac ctagtagtgt
    24001 aacagagttt atcagggctg ccaggatttc ggagcgtttt aacccgtagg tacgagtaag
    24061 agtggctttt tgtcctgata acctatttgc tgtgtaactt ataattagcg atagcacatc
    24121 gctgaagtta tgggctgcat cgctaagaag cgatatgctt cctgaaaata tgccgccaag
    24181 gaattcggca actgtaataa aaaggtttag aagtattgta atgcctaagt ttttcccgct
    24241 taccttatgg tggttatgat catggctcat gggtacaatt tttaagataa aattccgtaa
    24301 accatcttag ttgctatgag tagaagaaca acagcatata caacttttac ctgtttcgat
    24361 ttagcttttt gggtcatgta taaaccgccc agttgtgacc ctatgattac cgcaaggaca
    24421 actactatgg tgagtggcca atttatatgg ccctgggata cgtgccccag gtaacccgaa
    24481 aaggatgaga atgtcaccac aaatgcggtt gtggctgcag cctcctttgt tttgtagccc
    24541 atcatcatga gaattggtgc aatgataaag ccgccgccta aacccagcat gccaccgata
    24601 aaaccggcaa agccacccac aaagaaacct atgatacttc gtttctttaa cgacatcatg
    24661 gtttcgggct cctcctgttt tgacgcccaa agcgtgcgga gggcagcagc aacaaccatt
    24721 acagcaaata agaatttaag caattgaact ggaacatgtt cgctggtcat tgcgcctagc
    24781 ggggaagcta ctaatgcagt agcagccatt acggcaccac ccttccaatc aaccatcttt
    24841 ttacgggcaa acgggatgag taccaatgct gtgtttaaac cattcaaaag caaacccaaa
    24901 ggaattgcaa ccgtaaccat atcaaaccca gcccagttta gtactggtac gtaaaccatt
    24961 cctccgccta atcctagcat ggaaaaaatg aacgatgcta ttgaaattac tataaataca
    25021 attatgaaaa gcatattttt aaaattaaag tgagtatata cttacatgat gtgtaaaaaa
    25081 attacctggt gagcattttg tttatgagaa acatcatcct actacaaaag ttcgacgtgt
    25141 ctaaaacacc cttgttgagt attagttcaa cgttaagtga aacaggtatg cccatgtata
    25201 gcatggctac gttttcaacg ggtatgtttt cgtcccatat cccttcagca ataccgtcaa
    25261 gaacaacctt gcttaccatt tttttttgtg tattaaatat ctgcatcaag gcctgtttta
    25321 gctcttcatc gttattatgt gatgcttctg aaaagagtag tagggtaata cccttgttat
    25381 cggttaggta cttaacggta aagcaaagta agtgttccaa gcgtttttgt ggcagttcgt
    25441 tagaagtggc gatctttcgt aagtgttcaa taaaatctct ttgtacgtca gttataatgg
    25501 aatgaatgat gctttgcttt gttgggaaat gcctaaaaat tgcaccttca ctcatgccaa
    25561 tacgctgagc cagcatacga gtggataggt ttttcaatcc atcggtataa ataataccga
    25621 gaactgcctg cttaatctgt tcctgccgaa cctcagtggg ttgcctcata tatcaagtaa
    25681 gtaaacactt gcaaacatac aattccttcc ataaatacca caagaatcga tgttaaaatg
    25741 agttaaaaag aatggaggat tttattttac cccttttttc ttgagcactt caataagttc
    25801 gttttcgggg aaaaaacctt cgtgcctaaa gtattcctta ccattctcat ctaaaaaaat
    25861 ttgggtgggt ataaggttga tattatactt tttggcgtat tgctttccat cgtcagtcca
    25921 aacatcgtaa aagattacct ttacctgttt gccatacttt tcttctaccg attttagtat
    25981 aggctgcatt tgttggcaag ggatgcatct aactgaaccc aactcaataa aggttacctt
    26041 gtatttcctt tctgttttac tggtttggct tttggtttgc gcaaacgtat ttgatgaaag
    26101 agccgctata aaaaatgcgg taatggttaa ataaattact agtcgtttca ttgaactgtt
    26161 ttttttagtg ttaagcataa attttttctc taagaatagg tgtatcctaa gggttgttta
    26221 attccttaac aatggcctgt cgcacaattt cctcgtcggg aacttccctg gaggcaacat
    26281 atacacggtg taaacatttt ggattaatgg cttaccactt aaaagttttt cttaaaatcg
    26341 tcaaccttgt tggcaaggta gataatggca acaaaaacca atgcggctat aggaacaatt
    26401 atccattggt ttatgcccag cactgccgat atatcgccat taatgttttt ggtcatcagg
    26461 tttgacttga aaaacttcac attcactgca aagaatagaa tccccagaag cattccaagc
    26521 agtgctaccc atgcgtcttt tttggcttcg ccaatacggg caaacagcgt tcctggacag
    26581 taaccaagaa gacccatccc tgttccaaaa attaatccgc caaccacttt gccccaatct
    26641 agcgctttag gatgtagttc aataacaccc aagtttccaa gcatgtaaaa accaatcatc
    26701 gaaaaggtta ctgctgttag catgaacttt aggattttga aatcctttag cagaagcatc
    26761 cctgttaccc ttgggtactt tgtaaccccg gtacgttgta gcgcatagcc gaatattagg
    26821 cctattgcca atccaattaa aatttgtagt aacatgatta acgtcctcca gctttagggt
    26881 aaagtaactt tgcaacaata attccggcaa taaagaaaca ggctccagcc accatactac
    26941 caagcgatag gtgagcccag ccctgcagga tattacctgt tgtgcaacca acagccagca
    27001 tggtccctaa tcctaagcag aaacctccaa gcaacactac cccaaaacgt ttccatacgt
    27061 ttgtgccatg gtatttctcc caaacagcag gaattacctc tggggagtag tttttactaa
    27121 gctttgcacc aatgtatgcg cccagaatta tcccaaacag cattataaac tcaacaaaag
    27181 ctgctggact ggtccaattt ttctgaatta caggattatc ggcaagcgat acaaaaacat
    27241 acttaaaact tgcagcaatg taggctacac cagcagtaat gccaaagggg taatccttgt
    27301 agcttaactt ttcgaatagc acatatgtgg caatggatag tagtgagacc actatcccac
    27361 cagtaagcca gtgccattcg ccattttggg ttagtttgat tttcatagtt tggtttttta
    27421 ggttatcaat cactttaggt tactaaattt ataatttcat ttaatgcttt gcatgtattt
    27481 ctttatcgag tttaataaag gcttgtatgg caaaccaatt agagttttga gcagttgttc
    27541 cattgatggg ttgaaggtcg ccagcccaaa aatatccgaa aaccccaacg aactgaaatt
    27601 gtttgccagc attgtaactg gcaaaaaagt taaactcatt gccaaggtga tgtttgccgg
    27661 cttgaagttt cattgtgcca agcagcgttt tattgtcagg tgatgggagg tagaaaaagt
    27721 ggtagttcat ttcaatttta aatttctttg aaggggtaag atacaataca acctcgcggt
    27781 cgtcgagatt agaccaagat gtgatgttca ttcgtccgta gtagctatcc tttgccccaa
    27841 aagcagggtc gaaagtccga accctcgtat cgtcattatt tccaccactt gcataagttt
    27901 ccctcaagca cagagtaggc tttgctggta ggccatgaaa ctgatacccc aacaaaccaa
    27961 ttatgccaaa agcgttaatg gtctttccgt tttcgtgtcc aaactcctga gttagggtaa
    28021 aatcgtattt aaagcgattt acttcatcgc taaaaaccct tgtaccaatc cagtgtttag
    28081 aaaaactttg tttccggatg taatcggctg aaccttgcgt tttaattgcg tagaatggct
    28141 caaccactgc aagtttacca aattggatat gcgcataggc tccgccaccc caatgttcgg
    28201 tttcggtaaa tggtatagcc accttttcgg ggtcgtgagt tttggtgcct ccgccaaaca
    28261 catccaacga attattcttg tttctatagg atagtctaac tgcatcccaa gtccaacgac
    28321 cagtatttcc ccattcccca gggccaaaaa tccgtttatc gccaaacgaa atcttttgtc
    28381 ggccaatttt caggcttacc cccttaaaaa tctcttttac cgagaaatac aaatcataaa
    28441 tctcaaaaaa ctcctcgttg gggttcatgg tgtagtaggg ttcttgcgta ttcttctgct
    28501 ttactctgaa cagctctgga tactccgagt cccttaacga ccagccaaat gcccttgagt
    28561 cctgaatatg agccgcaaaa tcgattttgc ttgtgggttt gaacgtgaag cctgcaacaa
    28621 ttcgttgaaa cagtatttta tcgtttagtg agcctactcc attgggatcg ccgtaatttt
    28681 tggcattcat accgtcccac acctcaaaac gctcgcgtag ggtaatattg aattctaaaa
    28741 gggttgaatc tcctcctgtg ttggcatttg aaaaagttgg tagcacaagc tggcaagcta
    28801 aacaagttag cgaaaaaatt attcttactt tattcttaaa atataggcga agcattttct
    28861 tgtgcgtttg aataattaat gttgttaatt ccagtgggtt atcaataatg agttgttgat
    28921 ttgatttgtt gtgttctact ggtaatggtg ttgaaaacca atattattcc ctgattaata
    28981 gttggcatct agagctattc ataaccttgc atcagtatat aacttcaacc ttttcgtaca
    29041 ctttatttac aacctcttcg gttacagggg tttgaccttt tttgtaaccc aaatcggtta
    29101 ccctaaagta tttgtaatcg gtgaaaccat gtctatccaa tatacgttta ccgcaatcgg
    29161 tggggcagcc atcgagcata agaatatttc ttgatttgaa ggcctcgata gactgctcta
    29221 ttcccgctcc aaccactgca aggcaattca tcttacgtac accgttatct ctgagtttgc
    29281 gggcaaccaa atcggttatt tgtcctaaat cgcaagcgcc cgaacaggct aaaaccatgt
    29341 actcgtcaac gccacaaagg catcctgatt ttttgttatc ttccataaat agaaaaaatt
    29401 aaagtttata ctcaaaactt aacactatat gattctgaaa atcaacacta ctatctaggg
    29461 gtttccaacc ctgatagttt agtgaaatgt ttaccccctt caccgggttg tagtgaatac
    29521 ccgcaatgta agcatttcca attccgctgt aataccaact ttgtgcatcg ccgcttatgg
    29581 tgttggcctg tagctggtcg aaacggccaa atagctccac attctttgca acggaaacgc
    29641 caccgtaaac cgataatccg taaaggttat gtttattgat gtttaaatga ttcttgcggt
    29701 agttatactc tgcaccaatc ctgaatttat catcgaattt atacccagca aaaatagaga
    29761 atagctgttg ctctgatggt tttagcgggt tatccgattt cgtataatcg gtgtaaaagc
    29821 gtgtttgcag cccttttatc gggttaaaat caatgcctcc ggctatctta acatcgccgt
    29881 attcatcctg gtcgaaccga aagccttcac cgttggttat tgcaatatca aaaccgagca
    29941 tctcgttaag ggtaaaatat ccgattatcc ctaaatcgct actgggtatt ccgtaatact
    30001 tatcctgaaa ggtaggtgca acgtagcggt aaccccaaaa cttctcctga gtaatgtaat
    30061 ggttctgcaa aacgccgcca gcctgtattc taacgaagct attgggtttc cactcaatgg
    30121 atgcgaactt aagggtcatt gtgtagtagc tgccctcctt cgatttatcg gtaacatcaa
    30181 ggctattgcc cgatgtatcc cgcacttcaa tctgtccaac cgtggtgggg cgacctgcat
    30241 caataataat tttacccgag atttgcttgc tgaactggta ctcgtagcca aagtgtgcac
    30301 ggccaaacca aaatccgtag gtcttttttg cattgttggt ggcgttgtaa tcgaaattgc
    30361 caaaaacctg aattattggt ttgccatttg gtttaaatgt ggaatcggat gtttgagcag
    30421 ttacaatgca ttttgaaaaa aatacaagtg ctgcaaggag tgctggtttc ataactaaat
    30481 ttattgttat ggttgtttaa cttcaaactt tttccctttt aagtactttt ccatggcaaa
    30541 gccaatgagt atgaatattg gcagggttat tagagtcctc aggagcgaga atttgagtcc
    30601 caaaaatcct atctcgaagg ttatcatcgg aattttaatg gtggagtatg ccccaaggta
    30661 aataaatatg tttcgtatat tacagccctt aatccagagt agataggcaa agggaaaggc
    30721 tccgtaaagt gggccagcct gaagcgatgc caaaagaata accaggagtg ttcccttcca
    30781 gcccgagtcc tcaccaatat gtttttctat tttttctctg ggcacccaaa catcaaaaag
    30841 tcctataaga atgaacatca atggaaggaa gagtatcatc tccttaaaaa acatccagaa
    30901 atttaaggca atttgttccc cagctctatt gtcatacaaa tatgaaaatc ctataaataa
    30961 tagggaaaga ataggaagga agtattttat tatatttttc ataaaagaaa tatcattgta
    31021 ataccaattg ctatagctgc tacaaaactt aaaacattcc gtaggatact taccttccat
    31081 ccaaaaaact tggcttccac tggtagggtt aatatcccta ccattgtaag agtggtgatg
    31141 aatattgcga ccgtggagta ttcaactccg ttaacaataa ggatcttgca aagtgggaat
    31201 gcaataaatc ctgggataag cgataccgag ccaatgactg ccgcaataag gtagccttta
    31261 atgcctgaat ccttccctat taggcttacc agagtatcgt ttggaatgat aaagaacata
    31321 atactaacca gtgctagcat tgtaaccagg gttggtaaca atttaagaaa catctttact
    31381 ccccgagtaa ctcctttaaa ggttttttga gtatcgaaaa gcaacgatac cactaggagt
    31441 acagaaacta ctatggttaa cccaatcatt atattgtgtt tgagggttat aaattgaaat
    31501 caaaaaaact caattctgat tttgcatttc cccatgaaga ttcgggttat atgtgaaatt
    31561 gattggcttt agaacatcaa ttgcaaccat accagatgct atccgcatcc ggtgatggtg
    31621 caatacatac tttacgtatg aagttatcaa aatctttcat ggctaaaaga aatagccata
    31681 tataaggccc gaaatggttg ccataatcac aacaagcgaa acataagcca ctgcttttct
    31741 tgtgccaagc acgcccctaa ttacaagcat gttgggtagc gatagcgatg g
//
LOCUS       DAOOWJ010000020        31724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1445_length_31724_cov_7.331176, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000020 DAOOWJ010000000
VERSION     DAOOWJ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 31724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31724
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1445_length_31724_cov_7.331176"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1665)
                     /gene="rny"
                     /locus_tag="PLW22_03535"
     CDS             complement(130..1665)
                     /gene="rny"
                     /locus_tag="PLW22_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOU73809.1"
                     /translation="MVLTIIIAVSAFSLGGLLSYFSWNKLLASKRNKIIQEAEAEAEV
                     IRKEKILQAKEKFLQLKTEHDKVISEKNNRIAQAETKIKQRELQLSQKFEELQRKQKE
                     VDVIRENLSSQMELVEKRGEELERMHKDQIERLEAISGLSAEEAKAQLIESLKAKART
                     QAMSYINEIVDEAKMTATKEAKRIVVQSIQRVATETAIENSVTVFNIDSDEIKGRIIG
                     REGRNIRALEAATGVEIIVDDTPEAIILSAFDPVRREIARLALHQLVTDGRIHPARIE
                     EVVQKVQKQVEEEIIEVGKRTAIDLGIHGLHPELIRLVGKMKYRSSYGQNLLQHSREV
                     ANLCAIMASELGLNPKVAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPEVCN
                     AIGSHHDEVEMTTLIAPIVQVCDAISGARPGARREVVESYIKRLKEMEDLALSYPGVL
                     KTYAIQAGRELRVIVGSEKVSDEEANKLSFEIAKKIQDEMTYPGQVKITVIRETRAIS
                     YAK"
     gene            complement(1899..2207)
                     /locus_tag="PLW22_03540"
     CDS             complement(1899..2207)
                     /locus_tag="PLW22_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOU73810.1"
                     /translation="MDEKLSINVNVAERLYPLKIDRDDEEKIRKAAKLINDKVMLYKQ
                     RYSDKDVQDYLAMAALQFVIKLIECEDKTDTSEFMSELKDLDEWLGSFIGKQNMSSFK
                     "
     gene            complement(2224..2454)
                     /locus_tag="PLW22_03545"
     CDS             complement(2224..2454)
                     /locus_tag="PLW22_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73811.1"
                     /translation="MYEREKQENDRLKAQVAQLEAKLKAKEQACAELEERYSRLKIAK
                     ALKASDADVHEAKIKVNRIVREIDRCIALLNK"
     gene            2726..4417
                     /locus_tag="PLW22_03550"
     CDS             2726..4417
                     /locus_tag="PLW22_03550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU73812.1"
                     /translation="MRLFRFAGLLFLSSVTIAQTQPFKLPLEMPSAFSGTFGEVRSDH
                     FHSGVDLRTMGQVGYSVFAIEKGFVSRIKVSPVGFGKVIYIDHPNGKTSVYAHLDRFV
                     GKIAEYVENAQYQKQSFEIELFPNPGELNVNKGDIIGLSGNSGSSGGPHLHFEIRETT
                     SQHILDPFEFFNEWRQTDYNPPTINRLLVFEIDSLRYLLDSLTYKAYEAVKKGSDFIV
                     PLPIKASGKVGLALDYYDIVNSNSLRAGIKKAEVRINGNKTFTFRVDRFSFLETRYAN
                     GFNGITDNGWNGYKAIRLWTDPNNRFSGINHVINHGIIDIAKDSSYTIDVTVWDHSGN
                     SSKLIFKILGVENKVNATSVPSLPLFRWNAENTYKTDSYMVRLPEYALFHDILFQMEE
                     NQSDNLPYPTVTIHNPKTKLFKKFTLEYKASKIPENILSKHYIAKLNGKGFEYVGSEL
                     INGSLKATCNCFGKYTVLVDTIPPSIIAQNLMPSAKITSQNNINFKLIDNTGIKTYNG
                     YIDGNWVLFEWDPKTGILSHNLNPQRIKKGQWHSLKIVATDMLNNDSEYAIKFFW"
     gene            4451..5335
                     /locus_tag="PLW22_03555"
     CDS             4451..5335
                     /locus_tag="PLW22_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU73813.1"
                     /translation="MITVFYFILFLISIVIVAIIPWGLIYGYSNLMAKLLHNVLKYRV
                     RVTRSNLRLAFPEKSDAELAVIEKQSYQNLADISVEALKGFTMRNSAIRERHKILNPE
                     LLDYYYNQKQSIIGVAGHLNNWEWGALSAGIQLKHHPVAIYKPLSNKLIDWFIKWNRS
                     IRGTELVPTNQTFDTFQKYKHKPCIYILVADQSPSNLSKAYWINFFNQDTACIHGPEK
                     YAKMYNYPVIYIDIKRVKRGYYTVELSVLCDKPNELKDGELTAAYMHRLEQSIRENPE
                     SWLWTHRRWKRKRPQKAQ"
     gene            5392..6087
                     /locus_tag="PLW22_03560"
     CDS             5392..6087
                     /locus_tag="PLW22_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73814.1"
                     /translation="MKRYTSVLLAITLFFFSTQVFAQKYRNKEVGLIYFKVNIDPGYH
                     AKFSEYAHLFREPVKPQQDRIVGHLTADIWDIIKQQLTNDVGMIILPLDAYGDKFSYD
                     HYGFPNVTINRALQKGNSKFYISIDVEIGPEFMSKYVTKTSSDTSPQVIQLAPNEIKP
                     KITIKMNIYNDKGIIPVANTKGEAIESKVITLNDKYFDGFVNPNINSPNETLYKLVNQ
                     AVKELVISVYSRK"
     gene            6120..7166
                     /locus_tag="PLW22_03565"
     CDS             6120..7166
                     /locus_tag="PLW22_03565"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007144.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOU73815.1"
                     /translation="MIGLMSGTSLDGLDICYCFFQNINSKWQYYILAAETIKYPTHLI
                     NQLKEAHNYTAEEITALDFSYGKYLGEQVKRFVSEHGIEADYIASHGHTVFHNPTQGY
                     TLQIGKGAAIAAYSGIPCISDFRSGDVSRGGQGAPLVPIGDKMLFQEFDICLNLGGIA
                     NISYDDNFSNRVAYDICVCNMMLNHLAGLTGNEYDFNGDLGRTGQVIPGMLQELENIE
                     YYIQPPPKSLGREWFESYIMPILNRYNAYPLTSQLRTAYEHISGRIAHDCNSLKKTTM
                     LATGGGAKNVFLMELIKQKTELKIVIPDSKTIDYKEALIFAFLGVLYLLKEPGAIASV
                     TGANNNSIAGCLYS"
     gene            7249..9003
                     /locus_tag="PLW22_03570"
     CDS             7249..9003
                     /locus_tag="PLW22_03570"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOU73816.1"
                     /translation="MRKLTIQIFAAIALLINANTGNACTNYLVTKGASVDGSTMITYA
                     ADSHYLFGELYFKPAAVYPAGTLMKIYEWDTGKFLGEIPQTLQTYSVVGNMNEYQVAI
                     GETTYGGREELQDSTGLIDYGSLMYIALQRSKSAREAIKVMAELVEKYGYYSSGESFS
                     VADKNEVWIFEMIGKGTDIKVDKKTKKAYNANRGAVWVAIRIPDGYVSGHANHARITT
                     FPLENMKTSISSKNLDKIFEPDVEVVYAHDVISFARQKGYFTGKDEEFSFSDVYAPLD
                     FGAARFCEIRVWSFFKAVNKDMWNYFDYAKGHDLKNRMPLYIKPERKLSVRDMMNFMR
                     DHLEGTELDMSKDPGAGPHGLPYRWRPLTWKYEGKSYFNERATATQQTGFSFVAQMRS
                     WLPDAVGGIFWFSVDDAASTVYFPVYCSVTSVPEAYAEGKGDMLTYCDDCAFWVFNKV
                     SNFAYLRYDLMIQDIKRVQDELENRFISQTAVVDQTALKLLETNKEDAVRFLTDYTVN
                     TGNYVVARWEKLFQFLLMKFMDGNVKQEDGKGGLKYNQYDYCPAPVKNPQYPDWWKKN
                     VIDATGDKLAVPEGAASH"
     gene            9185..9898
                     /locus_tag="PLW22_03575"
     CDS             9185..9898
                     /locus_tag="PLW22_03575"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOU73817.1"
                     /translation="MEKIENSPKTDYSRLVLGFLLIVLAGLLLIDNFDLYPVEWGKYI
                     FTWQTLLIVIGLIILPGKGNRTTGTVLIAIGAFFLAAKFYELPVSISKLFWPAVILFI
                     GLSLIFGSRRRRWHPYKTGEVKDDLIDDVSIFGGSDQKFTSQQFRGGKITNIFGGSTI
                     DLSTAKLESGLNVVDLLCIFGGSKLIIPSEWKVKIEVTSIFGGVSDKRKIPQALPENA
                     PELVLKGLVLFGGVDIKSY"
     gene            10056..12113
                     /locus_tag="PLW22_03580"
     CDS             10056..12113
                     /locus_tag="PLW22_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOU73818.1"
                     /translation="MITNELINPKSIVVVGGSDDIQKPGGKVLKNLIDNHFKGSLYVV
                     NPKADEVQGVKSVRDVAELPQVDLAILAIAAKFCPQTVEVLATQKGTRAFIILSAGFH
                     EESEEGAKLEQQIVDTINRTGGCLIGPNCIGVMNTNYAGVFTTPIPRFDPKGVDFISG
                     SGATAVFIMESSIPRGLTFNSVYSVGNSAQIGVEEVLEYLDNTFDPQSSSRVKLLYVE
                     SINKPLKLLKHAQSLIRKGCRIAAIKAGSSSAGSRAASSHTGALASSDVAVEALFRKA
                     GIVRCYGRDELATVASIFMHPELQGKNIAVITHAGGPAVMLTDSLSNNGLEVPPIEGP
                     KAKELLAKLFPGSSVANPIDFLATGTAEQLGFIIDACENDFHNIDAMIVIFGSPGLFP
                     VYDVYDLLDEKMKVCRKPIFPVLPSVMNVKNEIEHFIAKGRIFFPDEVSLGNALAKIY
                     HTPKPSPEAPVLPKVNEKAISEIISKSNNGYLTPDAVQQLLDAAGIPRAGEAVVTTAD
                     DAARAAEKLGYPVVMKVVGPVHKSDVGGVVLNVKDSETVRKEFDRMIKIKDTTAILIQ
                     PMLKGVELFVGAKREDKFGHMVLCGLGGIFIEVLKDVKAALAPIDTNEALNMIKGLKS
                     YGIIKGARGQEPVNEQIFAEIVSRLSALVTVAPEIFEMDLNPLLGSKDKVVAVDARIR
                     IEK"
     gene            12120..12269
                     /locus_tag="PLW22_03585"
     CDS             12120..12269
                     /locus_tag="PLW22_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73819.1"
                     /translation="MSLKEKWNSLTANEKMMIGLAIILVILIATRFEYIWDQVKAGFA
                     RYFNR"
     gene            12306..12977
                     /locus_tag="PLW22_03590"
     CDS             12306..12977
                     /locus_tag="PLW22_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mpv17/PMP22 family protein"
                     /protein_id="HOU73820.1"
                     /translation="MVGTILFLLPFFISETVYNFYVDFNASHGMVTSFIKFALLATFG
                     EAIGLRIRTGNYNQPGFGLLPRAVVWGLLGLTIKLAFVLFASGVPQFLAYMGMENAPQ
                     ALKGELTLAKILVAFSISLFMNIIYAPVMMTLHKITDTHIVNNGGTLSCLLKPIHFGN
                     ILASINWEVQWSFVFAKTIPYFWIPAHTITFLLPADFQVLFAAVLSVALGIFLAVASL
                     KGSGK"
     gene            13025..13543
                     /locus_tag="PLW22_03595"
     CDS             13025..13543
                     /locus_tag="PLW22_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOU73821.1"
                     /translation="MNKRIGIFYGPTGGSTEKVAQLIAKELGAIADLHPIKESTPSMV
                     NNYDIIVFGCSTIGSETWNGEASRSDWDHFRPELKKIQVQGKLFAFFGTGDSVTYPRN
                     FVDGMGILAKELIAQGAQVIGQWPTDGYSFTDSEALINGKFIGLPIDEDYEADKTIAR
                     VKGWVELIKKSI"
     gene            complement(13601..14389)
                     /locus_tag="PLW22_03600"
     CDS             complement(13601..14389)
                     /locus_tag="PLW22_03600"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOU73822.1"
                     /translation="MKRLFWVLTSVILLSSCSDDGINFFTLEQDIQFGAQLDSAILAN
                     PNEYPILDRTQYAEAYTHIERVMNAILSSDDLRYTTTFPWQVKIIHDDNVLNAFAAPG
                     GYLYFYTGLIKFLDNEAQLAGVMAHEIAHADRRHSTQMLTRQYGFSMLLSIIMGDNPS
                     QLEQILSQLALGGTMLKYSRDNEYEADKYAVTYLCDTDYHPRELAGFFEKMMGSTQKI
                     PEFLSTHPSDENRIKNIDDVWRSLGSKVGNTYADRYQSFRNSLP"
     gene            14587..15276
                     /gene="mtgA"
                     /locus_tag="PLW22_03605"
     CDS             14587..15276
                     /gene="mtgA"
                     /locus_tag="PLW22_03605"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOU73823.1"
                     /translation="MLVRVITYSVVLFFTLSISLTVVYKWVNPHYTWLMILKKHLPET
                     QGGSSNFEQSWVSIEEISPQMVLAVIAAEDNKFMEHNGFDWESIKKARKYNQRGKKLR
                     GASTISQQTAKNVFLWPKRSWVRKGLEAYFTLLIETFWSKERIMEVYLNVAEMGRGIY
                     GVEAAARKYYGKPASRLTRHEAAMIATTLPAPAKRNPSKPSAYMVSYQRKILWNMYNL
                     GEIDINEKEQK"
     gene            15299..17230
                     /locus_tag="PLW22_03610"
     CDS             15299..17230
                     /locus_tag="PLW22_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73824.1"
                     /translation="MQRLLFPFWLIVTFLVCFTPSKAQFNKAYFFYKGEELISRGNYA
                     DAIPVLNTLLNVDSTIHEAWFLRGVARYYQNDLVGAGNDFSKCLNINPLFSQAYQYNA
                     MVLQQLGKSVESIECIQKAIELRPASAQYLFTYGVILFQQEQYHDALYAFNQVLKIDD
                     RIPDAWVNRGTTRLMLSDSSGALTDYSAAINLNPFNANPYLRRGALYAQQSNYPLALQ
                     DLNQAIGIDSTLAQAYFTRALVFYYQKHYSQALSDLNKVLQLEHRNTLALFNRAIISY
                     EVGNPESAIDDLTRLSSIIPTNVLVHYYLASIRYELGYLPMALENINKAIAIFPEFAN
                     AYRLRAEIKTKKGDLKGAEADIAQGNRLAQAYSNRAINDIASVIDSTGKLKRLLSLDE
                     ELDITSSISMEQLRTRILTIVYPIITLKVDKIENVKPRNWQSSNLRQILATQSCRGYS
                     LFFEYDTINQHSQLETIATDSLSEICKAIAHLQGNYYTAAMKEFSETLKADSSNTLYR
                     LAEVLAKIKMTQFVENVKNKSLQSGKSNISQKTIDNKPYITAGNDLKTIALDNDFEGI
                     VPYNIGVMFMHTENNDLAIEWFSKAISINPLIHGAWYNRGLLYLINNETVKGCADLRK
                     AVNIGNNQASEVVARFCKK"
     gene            complement(17280..18707)
                     /gene="hydG"
                     /locus_tag="PLW22_03615"
     CDS             complement(17280..18707)
                     /gene="hydG"
                     /locus_tag="PLW22_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOU73825.1"
                     /translation="MKFNPEKYRIPDERMKPFIDADEIWEYINNAKPTKERVREIIAK
                     SLDKNRLNLEEVATLVNTTDPELVEEILQGARTLKERIYGNRIVLFAPLYIGNKCTNN
                     CQYCGFRASNKDAIRMTLSDEEIVQEVEALEDNGQKRLILVYGEHPEYSAEYIAHTVR
                     LVYGIKKNNGEIRRVNINAAPLDIEGFRKVKEAGIGTYQIFQETYHPEAYTWYHPSGK
                     KKDFEWRLTSLDRAQEAGLDDVGIGALFGLYDWRFEVLGLVRHTNHLEACYNVGPHTI
                     SFPRIKSASGLNITDKYFVSDSDFVKLVAILRLAVPYTGMILTAREPADIRRQVIQFG
                     VSQIDGGTKLELGSYHASKNEHQDLNKEQFEINDNRSLGEIIDELIDHNYIPSFCTAC
                     YRLGRTGEHFMEFSVPGFIKRYCTPNALLTLTEYLVDYANPEVQQKGYKLIEKTLGEL
                     DGHQNTLEIRRRIERIKAGERDLYF"
     gene            complement(18791..19039)
                     /locus_tag="PLW22_03620"
     CDS             complement(18791..19039)
                     /locus_tag="PLW22_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOU73826.1"
                     /translation="MEKRIGAALILVENRESASRLNQILSAHADIIIARQGIPLQQRG
                     ISVISLVLEGTTDEIGALTGPIGKLSGVQVKTLMLKGV"
     gene            complement(19209..20153)
                     /locus_tag="PLW22_03625"
     CDS             complement(19209..20153)
                     /locus_tag="PLW22_03625"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOU73827.1"
                     /translation="MKVHYGFDGIGEITNPVVTTGSFDGVHLGHKVIINRINEIAQSI
                     GGESVLITFYPHPRKVLYPETAGKNLMFILSQREKIELLSKTGLDHLIIVKFTLEFSK
                     ISSVQFIRDYLISKLNARYIVVGFNHHFGHNREGDYEELKKLSSEYNFMVEEIPEQDI
                     QQETVSSTTIRKALLEGKIQRANAYLDHYYMIIGALGKGSHFFGEVGFPTLTVQIEEA
                     GKLIPPEGIYAVSLEWNKSSYRAMVIIWSNGDNTDLNPVLTRNVELHILNFDGMLHSS
                     DAYIYFHKQIADRIDISDNRLLFQQLTQAAKQVDELIY"
     gene            complement(20163..20798)
                     /locus_tag="PLW22_03630"
     CDS             complement(20163..20798)
                     /locus_tag="PLW22_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOU73828.1"
                     /translation="MDKANLPEKTIERLSKYRRTFLNCLTRGKTHIYSHELAGLHNIT
                     AVQVRRDLMLIGYSSMKKKGYDAQELIKVIGDIIDHKVGLNVAVVGMGNLGRAITTYF
                     NGKRPKLNIVAAFDVDPNKVNRVISGVNCYHMKELPDVIKRNDISIAIISSPPDTANQ
                     VAEQLVAAGIKGILNFTTIPLVVPENVYLEEYDMITSMEKVAYFVKQGYSR"
     gene            complement(20878..22026)
                     /locus_tag="PLW22_03635"
     CDS             complement(20878..22026)
                     /locus_tag="PLW22_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOU73829.1"
                     /translation="MEVVDILVVDDEPGIRSGVTRILRNFTVSYPFLEDDIGFNVIEA
                     ATGEEAIDIIKRSSPAIVLLDNKLPGIQGVEVLQFINQNHPDILVMMITSYASLELAV
                     RATNIGTYDFVPKPFTPQELKSSIENITKHYFLRRMTRKLHREGKQVRFQFLTVLSHE
                     LKAPLNAVEGYLKMMQEKQAGESIDSYMDFIDRSLARIQSMRNLIMDLLDLTHIESGK
                     RQRTLREIDLTLVARSAIETMTPLAIQRDVNLSLQTTEPVIMLADSDDMEILFNNLIS
                     NAVKYNKPGGWVKCSISKTASEVTLVVSDSGIGIEPEDIPKLFQEFTRIKNPQSKNVT
                     GSGLGLSIVKRVVDLYGARIDVQSEPGAGSTFTITFPLPGSDTLNDGI"
     gene            complement(22029..22424)
                     /locus_tag="PLW22_03640"
     CDS             complement(22029..22424)
                     /locus_tag="PLW22_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU73830.1"
                     /translation="MANSRKTILIVDDDTDYLFQLKFHVEKMGFDVVTAESQREAENI
                     LKNIKPDLAILDLMMENEDSGFILSYKLKRMYPDVPVIIATAVSAETGMSFGISSEQE
                     RQWIKADLYLEKGIRPDQLHREILKLLKV"
     gene            complement(22440..24449)
                     /locus_tag="PLW22_03645"
     CDS             complement(22440..24449)
                     /locus_tag="PLW22_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU73831.1"
                     /translation="MEMNREKRKLVYTIKELCRVCYTCVRECPAKAIKIVNGQADVIS
                     ERCIGCGNCVKVCSQDAKVFLLTRTEVKSMLQSGERVAALVAPSFPAEFTEIDDYRYF
                     VGMLRALGFSHVFEVAFGADLVASRYKSLLEDRSGKGYISSDCPAIVTYIRYYHPDLV
                     ENLAPVVSPMVAISRVVRQIDSNLKQVFIGPCIAKKDESIEIDEAITFRELRVLFEQA
                     SITPESVEPSDFDPPHAGRGAIFPVSRGLMQTVNIFDDLIEGNITVAEGRIGFQEAIK
                     EWESGNLKGQNLELLCCEGCIMGPGMSPGGKRFERKTYISSYARNKVTPEQLKEWELA
                     ISKFKDLDLTRSFNPDDKRIAKPTDDEINKVLAKMGKFTPRDHLNCGACGYDTCTEHA
                     IAIVEGLAEVEMCLPYAIEELHESINDLAISNEKLAKMQQALKHSEKLAHMGQLSAGI
                     AHELNNPLGVVLMYSNILLDECKPDDPLRKDLELISSQAERCKKIVSGLLNFARKNQV
                     KVEKVNVKKLAEDSIAGVVIPKNVTTNVVCRTTNLMASLDYEQMMQVLTNLNKNAFEA
                     MPNGGDLTIVIDGDDHQISISVIDTGEGIPEENMDKLFTPFFTTKGIGKGTGLGLATT
                     YGIVKMHKGKIDVESNTDPQKGPTGTTFRITLPRNAENELGNDNS"
     gene            complement(24471..26195)
                     /locus_tag="PLW22_03650"
     CDS             complement(24471..26195)
                     /locus_tag="PLW22_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOU73832.1"
                     /translation="MNITIEVNNRTIQAHKGDTILEALNRNGIRVPTLCNMTDLTPSG
                     ACRLCVVEVEGRDNLVTSCSHVVENGMRIMTHSPRVVRARKTLVELLLANHPDDCLYC
                     ERNGFCELQDLAAELHIRERKVLRKSFTQKLDLSSPSIVRDPAKCILCGRCIRVCDEV
                     QSVSTFEFVNRGCDMYVGTTLSRDLNFSNCIGCGQCVLVCPTGALHERQNVAEVMDAL
                     SNPDLIPVVQIAPSISVSVATEFGLKPSKDVNGLLYNALRKIGFKYVFDTAFAADVAA
                     IEMAAELIERKGNGSNLPMFSSCCPAWVKFVEQWYPDYIPALSAVKSPQQVMGSLIRN
                     YFAAENELNPSKIFSVSVMPCLAKKFEAQREEMTTRGLSDVDTVLSTRELVRLIKLYG
                     IDIQGIESQHPDKPFNIRSSSGKLFGISGGSAEALARVVYHKLTSKELTDSKVNEVKI
                     LSGVKAFSFNAAGQTFTFAVVNGLKNIHSILPVLMGSDVKLDYIEVMACHGGCVNGGG
                     QPYVDEEKYVKLRAKAIIEIDEAEGIKCPTRNPAVQYIYDEFLGETRSEKAKKFLYTR
                     YSKREVLL"
     gene            complement(26209..28158)
                     /locus_tag="PLW22_03655"
     CDS             complement(26209..28158)
                     /locus_tag="PLW22_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOU73833.1"
                     /translation="MARCDSKAEISFIKDAVIPNLHDGVWLTEVNAKLSALRHERVRQ
                     PIIYVGVTTSSIVAGALKTRSAIEQYLDDYSVDADVICVGSHGLCSLEPIVEVQMPGR
                     TRVAFANVTPERVNSILDGALNGFIQPENAIFQYYSEIHEPWHGIPFRNELPFFKRQN
                     RVLLKNAGIICPDSIAEYIAHGGYSALAKSIRKYTYDDICRIVEESGLRGRGGGAFPT
                     GTKWRKALQVATSDRYLICNADESDPGGFMDRLLIESDPHRVVEGIILSSYAVSASKA
                     IIYIRDRYKITIQRIQNAIDQAYEVGLLGYDILGSGYSLDIEVRKGPGAYVCGEETAL
                     IKSLEGKRGMPSIKPPYPAEFGLNGKPTVINNVETLANIPEIISNGPQWFASLGTEGS
                     KGTKIFSINGQASQTCMVELTFGEPLGALLDLAGGVKDGRTFKALHLGGPSGVSVTAD
                     ELDAPIDFDELPKRGISLGSGGVQVFDNTVCMVDLVKYFMHFIRNESCGKCIPCREGS
                     NRMYQILDNISKRPVSNEGHNTLERFKGVIQLETLAEVMRDTSLCGLGQTAPKPILKA
                     LKSFRDEFEEHIFDRKCRASVCRDLRTFYIDIERCTGCTACAKKCPTQAIYGTPRSPY
                     FIVEERCIGCGICQDTCKFGAVFFK"
     gene            complement(28161..28625)
                     /locus_tag="PLW22_03660"
     CDS             complement(28161..28625)
                     /locus_tag="PLW22_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU73834.1"
                     /translation="MSNAEKIEEILERYSYLGSDSLIPILQDIQNELGSLNEEAIVLV
                     GRKLNMPTSKIYGLATFYNQFKFEAKGKNHICLCNGTSCRLKGAKWNMVYLEDNLKLK
                     NGQTTRNGQFSLEVVTCMGACEHGPVISVNDEYYREVDPEKLKNILNDLTQE"
     gene            28927..29172
                     /locus_tag="PLW22_03665"
     CDS             28927..29172
                     /locus_tag="PLW22_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOU73835.1"
                     /translation="MSKRAELIICLGSSCFARGNKATLKAIMKFLDDHDLKDKVNFHG
                     GHCFGNCAEGPNISINGKIYTGVDENGIIDILTNELL"
     gene            29181..31052
                     /locus_tag="PLW22_03670"
     CDS             29181..31052
                     /locus_tag="PLW22_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU73836.1"
                     /translation="MSQLFRIDEQTCINCYACVRVCPVKAIEVKAGQNYARIIDSRCV
                     GCGSCLNICPVNAVSYRSSKKEVLELLKGDEPVAAICSPSISGEFVDITDYRKFVQMI
                     KQLGFTYVCEVTFGVDLVAREYKKLFEDFKGKYFITSMCPVVVSMVEKYHPEIAQNLA
                     PIISPVVATAMAVRKEYGSNVRVVLIGPCIQSKEEIRLYDDERRIDENLTFVELRELF
                     DEFNIKESNLEYSEFDEPIGYKGSLFPIGRGILQAVDISEDLLTGTVISTNGPNNTLQ
                     ALEQFETSIDLINRHFNLFYCEGCLMGPGTSRGGKKFIRRTMVVNYANKRLKTFDREK
                     WEKNIAKNSLGNFARGFTIDDQRLPTPNEHKLEEVLKVIDKEFVGRDSGCEACGYSSC
                     YDFASAVASGLAHTDMCLNFTLKKRQEYIKTLQATNEKLAKTQEALKESEKKARTEQE
                     AAREATETITAMLKKLPSAVVLIDKKMKILQSNQSFIDLLGDDALEINEIIPGLVGAD
                     LKTLLPYNIHNLFTSVLQNNTDILNRDVMYKDKLLNLSVFTIKPNSIVGAVIRDMYAP
                     EVQKEEVIKRITEVIDKNLAMVQNIGFLLGEGASETEKMLNSIIKTYREGKNNDDKA"
     gene            31065..>31724
                     /locus_tag="PLW22_03675"
     CDS             31065..>31724
                     /locus_tag="PLW22_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU73837.1"
                     /translation="MSSKFFIEVNCQQKNHDEERICGDVFYSRRLQDEERTIIVLSDG
                     MGHGVKANVLATLTSTMALNFTIEHKEVNTIAEIIMNTLPVCSVRKMSYSTFSIVDID
                     HNGETRILEYDNPQCLIMRGTEELEPEWQCIILNSEKNAGKELKFCSFVPQKEDRIIL
                     WTDGVAQSGLGSADYPFGWGDEAREFVKRMVKNEPDISARKLSTKVVNMASLNDGYHP
                     KD"
BASE COUNT         9300 a         7018 c         6608 g         8798 t
ORIGIN      
        1 ggtcgcacat ctggatgttc atagatttaa ggttaaaggt tcaaagttca aggataaagg
       61 ataaaggata aaggataaaa gttaaaggtt cacatttaat actgtacact gcacacttta
      121 cactaaacac tacttagcgt agctgatggc tcgggtttcg cgaatcaccg taattttaac
      181 ctgacctgga tatgtcatct cgtcctgaat ctttttggca atctcgaacg agagcttatt
      241 ggcttcttcg tccgacacct tttcgctacc aacaattaca cgcaactccc taccagcctg
      301 aattgcatat gttttaagca ctccggggta tgaaagcgca aggtcttcca tctccttaag
      361 tcgcttaata tacgattcaa caacctcgcg acgtgcacct gggcgtgcac ctgaaatggc
      421 gtcgcaaacc tgaacaatag gagcaatgag ggtggtcatc tccacctcat cgtggtgcga
      481 gccaatagca ttacaaacct cgggtttctc cttgtacttc tcggcgagtt tcataccaag
      541 aattgcgtgt ggtagttcgg gttcatcatc gggtaccttg cctatatcgt gcagtaagcc
      601 agcacgcttg gcaaccttgg ggttaagccc aagctctgaa gccataattg cacaaagatt
      661 tgcaacctca cgcgagtgct ggagcaggtt ttgcccatag gatgagcgat acttcatttt
      721 accaaccagg cggatgagct caggatgtaa gccatggata cccaaatcga tagcggtgcg
      781 tttgcccacc tcaataattt cctcctcaac ctgtttttga accttttgca ctacctcctc
      841 gatacgcgcg ggatggatgc ggccgtcggt taccagctga tgaagggcaa gtcgggcaat
      901 ttcacgacgc acagggtcga aagctgaaag gatgattgct tcaggggtat cgtccacaat
      961 gatttccaca ccggtagcag cctccagggc tctaatgtta cggccttcgc ggccaatgat
     1021 gcgacccttg atttcatcgg aatcgatatt gaacacagta actgagttct caatggccgt
     1081 ttcggttgca acgcgctgaa tgctttgtac cacaattcgt tttgcctcct tggtggcagt
     1141 cattttggcc tcatcaacaa tctcgttgat gtacgacatg gcctgggtgc gagccttggc
     1201 tttaagcgac tcaataagct gagctttggc ctcctcggcc gaaaggcccg aaattgcttc
     1261 aaggcgctca atttgatcct tatgcatacg ctcaagctcc tcgccacgtt tttcaaccag
     1321 ctccatttgg ctcgagaggt tttcgcgaat aacatcaacc tccttctgct tacgctgaag
     1381 ctcctcaaac ttttgcgata actgcagttc gcgctgcttg attttggttt cagcttgagc
     1441 aatccggttg tttttttccg aaattacctt atcgtgttca gttttaagct gtaagaactt
     1501 ttcctttgcc tgcaaaatct tctccttacg gataacttcg gcctcggctt cggcctcctg
     1561 aataatctta ttgcgcttgc tggccaaaag cttattccag ctaaagtacg acagtaatcc
     1621 ccctagggaa aaagccgaaa ctgctattat tattgttaac accatactaa atgaattttt
     1681 aagaattata tatataaaaa acccgcatta tgccttaatt ttatgtagaa accccagcta
     1741 aacatgggtg gagaggccat ccgattcaga ctaccagcgt ctcccggagg agatcccgaa
     1801 caagcggtaa acaggcctgt cttgtgcgga gaaagtcctt ctccaatttt tgtagtgttg
     1861 agtttcgcaa aaacgttaag gcgataatgc gggcaatgct atttgaaaga actcatgttt
     1921 tgcttaccaa tgaaagaacc cagccactcg tccagatctt tcaactcgct cataaactcc
     1981 gaggtgtcag ttttatcctc gcactctatc agctttataa cgaactgtag tgctgccata
     2041 gccaaataat cctgtacgtc cttgtcgcta tacctttgct tgtacagcat taccttgtcg
     2101 tttatcaatt tagcagcctt acgtatcttt tcctcatcgt ctctgtcgat tttgagggga
     2161 tataaccgtt cggccacatt cacgttaatt gataactttt cgtccattcg gcagcgctag
     2221 gttctattta ttaagtagag caatacacct gtcaatttcc cgcacaatcc ggttcacctt
     2281 aatctttgcc tcgtgcacat cggcatcgct tgccttcagc gccttggcta tctttaaccg
     2341 ggaatatcgt tcttcaagct cggcgcaggc ttgctcttta gctttcagtt tagcttcaag
     2401 ctgagcaacc tgagccttta aacggtcgtt ctcctgtttt tctctttcat acataaaaac
     2461 aagagtttga accttttgtt taatgttgtc gattatatgg ctcgaagaat cgttcatatc
     2521 ccagataaag ttttacaaag ttaattaaac ctacttcaaa aaacaaatct ttaaacaatt
     2581 tttaaactca gcagagtggg gttttccata aatctaataa tgaaatgctt acgctcaaaa
     2641 ctaaaatatt tctatcggtt catttcaatt ttaagcacca ctgaaggtag gcttttatat
     2701 atttgggcat aaaaagataa taacaatgag gcttttccgc tttgcaggtt tgctatttct
     2761 ttcaagtgtt acaattgctc aaacacaacc gtttaagtta ccactcgaaa tgccatccgc
     2821 cttttcgggc acatttggtg aagttcgatc ggatcatttc cactcagggg tcgatcttcg
     2881 taccatgggg caggtaggtt atagcgtttt tgccattgaa aaaggttttg tttcacgaat
     2941 caaggtaagc cctgttggtt ttggtaaagt aatatacatc gatcacccca atggcaaaac
     3001 atcggtatat gctcacctcg ataggtttgt gggtaaaatt gcagagtatg ttgagaatgc
     3061 ccaataccaa aaacagtcgt tcgagattga gctcttccct aacccaggag aactaaatgt
     3121 gaataagggc gatattattg ggctatcggg caacagtggt agttccgggg ggccacacct
     3181 gcacttcgag attcgtgaaa ccacctcgca gcatatttta gatccgtttg agttttttaa
     3241 tgaatggagg caaaccgatt acaatccacc aaccattaac cgtttgcttg tttttgaaat
     3301 tgacagctta agatacctgc tcgacagctt aacctataaa gcctatgaag ccgtaaaaaa
     3361 ggggagcgat tttattgttc cattgccaat taaagcatcg ggtaaagttg gcctagcact
     3421 cgattactac gatattgtaa attcaaacag cctgagagcc ggtatcaaga aagccgaagt
     3481 gagaataaat ggtaataaaa cttttacctt ccgtgttgac cgattctctt ttctggaaac
     3541 ccggtatgca aatggtttta atggtattac cgataatgga tggaatggct ataaagcaat
     3601 tcggctatgg accgatccta acaacaggtt ttcgggcatc aatcatgtta tcaaccatgg
     3661 tattattgat atagctaaag atagttctta cactattgat gttacggttt gggatcattc
     3721 aggaaacagt agcaaactaa tattcaagat attaggcgtg gaaaataagg taaacgctac
     3781 aagcgtacct tcccttcctc ttttcagatg gaatgctgaa aatacataca aaaccgatag
     3841 ttacatggtt aggttgcccg aatatgctct attccacgat atactattcc aaatggaaga
     3901 gaaccagtcc gataatctac catacccaac agtcactatt cataatccaa aaacaaagct
     3961 cttcaaaaag ttcacattgg aatacaaagc cagcaaaata cctgaaaaca ttttgagtaa
     4021 acactacatt gcaaaattaa acgggaaagg atttgaatac gttggaagcg aacttattaa
     4081 tggtagccta aaggcaacct gcaattgctt tgggaaatat accgttctgg ttgatacaat
     4141 tcctccgtca attatcgccc aaaacttaat gcctagtgca aaaatcacca gtcaaaacaa
     4201 cataaacttt aagctaatag acaatacggg gatcaaaacg tataatggct atattgatgg
     4261 taactgggtg ttatttgagt gggatccaaa aaccggtata ctttcgcata atcttaatcc
     4321 ccaaaggatt aaaaaaggtc aatggcatag ccttaaaatt gttgctaccg atatgttaaa
     4381 caatgattcg gaatatgcta tcaagttttt ctggtaatag ctaactttga catagtaaac
     4441 tgattgagtt ttgataaccg ttttttactt catactattc ctcatctcaa tagtcattgt
     4501 agccataatt ccatggggct taatctacgg ctacagtaat cttatggcta aactcctgca
     4561 caatgtttta aaataccgtg taagggttac ccgaagcaat ttacgattgg cattccctga
     4621 aaaaagcgat gcggaattag cagtaattga gaaacaatcg taccagaacc tagccgatat
     4681 ttctgttgag gcgttaaaag ggttcaccat gcgcaactca gccataagag aacgtcataa
     4741 aattcttaac cctgaactac tcgattacta ctacaatcag aaacagagta ttattggagt
     4801 tgcagggcac ctaaacaact gggaatgggg agccctctcg gccggtatac agctaaaaca
     4861 tcacccggtt gccatataca aaccgctaag caacaagctt atcgattggt tcatcaagtg
     4921 gaaccgatcg attaggggaa ccgaattggt tccaaccaat cagacctttg atacctttca
     4981 gaaatataaa cacaaacctt gcatatacat tctggttgct gaccaaagcc cgtcaaacct
     5041 atcaaaagca tactggataa actttttcaa ccaggatacc gcctgcattc atggtcccga
     5101 aaagtatgca aaaatgtata attaccctgt tatatacatc gatataaaaa gggtaaagag
     5161 gggttactat actgtagaac taagcgtttt gtgcgataaa ccaaacgaac tgaaggacgg
     5221 tgagttaacc gctgcctaca tgcacaggct tgaacaaagt ataagagaaa accctgaaag
     5281 ctggctatgg acgcataggc gatggaaaag aaaaaggcca caaaaggcac aatagaacta
     5341 atttggttca ctttttgata agttttatta catttgaaaa aaattcaagc catgaaaaga
     5401 tatacttcag ttctgctggc aataacttta tttttcttta gcactcaggt ttttgcccaa
     5461 aaatacagaa ataaagaggt agggctgatt tatttcaagg tcaatattga tcccggatac
     5521 catgcaaagt ttagcgagta tgctcacctt ttccgcgagc cggttaaacc acagcaggat
     5581 agaattgttg gacaccttac tgccgatatc tgggatatta ttaagcaaca gcttaccaac
     5641 gatgttggta tgattatatt gcccctggat gcctacggcg ataaattttc gtacgatcac
     5701 tatggatttc caaatgtgac aataaacaga gctcttcaga aagggaactc aaagttttat
     5761 atcagcattg atgtggaaat tggccctgag tttatgtcga aatatgtaac caaaacatcc
     5821 agcgacacct caccacaggt aatccaactg gctcccaatg agataaagcc aaagattacc
     5881 attaaaatga acatctataa cgataaagga ataataccag tagccaacac taaaggtgaa
     5941 gctattgaaa gtaaagtaat aacgctgaac gataagtatt ttgacggttt tgtaaaccca
     6001 aacataaact caccaaacga aaccctttat aaactggtca accaagctgt gaaggaacta
     6061 gtaataagcg tgtattcgcg taagtaatgc attccccaaa caagtcttca ggttatagga
     6121 tgataggctt aatgtcgggc acatcgctcg atgggctcga tatttgttac tgcttttttc
     6181 agaacataaa tagcaagtgg caatactaca ttttagctgc cgaaaccatt aagtatccaa
     6241 cacatctcat aaatcagcta aaggaagccc ataattacac tgctgaggag attaccgctt
     6301 tagactttag ctacgggaag tatttaggtg aacaggttaa acggtttgta tcggaacatg
     6361 gaattgaggc cgattacatt gcatcgcatg gacacaccgt ttttcataat ccgactcaag
     6421 gatacaccct acaaattggc aaaggtgcag caatcgctgc gtattcagga attccttgca
     6481 tttccgattt tcgttcgggc gatgtttcac ggggggggca aggtgcaccg ctagttccaa
     6541 ttggagacaa aatgcttttt caagagtttg acatctgcct taatttggga ggcatagcaa
     6601 atattagcta cgatgataat tttagcaacc gggtggctta cgatatttgt gtttgcaaca
     6661 tgatgcttaa ccacctagca gggttaacag gtaatgaata cgattttaat ggtgaccttg
     6721 gaagaaccgg tcaggttatt ccgggaatgc tccaggaact tgaaaatatt gaatactaca
     6781 ttcagccacc gccaaaatcg ttgggtaggg agtggttcga gagctatata atgcctatac
     6841 tcaacaggta taatgcatat ccgcttacca gtcagttaag aaccgcatac gagcatattt
     6901 caggtagaat agcccacgat tgtaacagct tgaaaaaaac aaccatgctg gcaaccggtg
     6961 gaggtgctaa gaatgttttt ttaatggagc taattaagca gaaaaccgag ttgaaaattg
     7021 taattcccga tagtaaaact attgattata aggaagccct gatatttgca ttccttgggg
     7081 tgctttactt actaaaggaa cccggcgcca tagcctcagt aacaggagca aacaacaact
     7141 ccatagccgg gtgtctatac agctaaaacc ccgaaccatt aaaccatcaa cctggtttat
     7201 aatcaaaaaa atattaggtt tgtaaacttt aattaaacta aaacgattat gagaaaactc
     7261 acaatccaga tttttgctgc tattgcatta ctgattaatg ccaataccgg caatgcttgc
     7321 accaattacc ttgtaaccaa aggtgcatcg gttgacggct caaccatgat aacctacgcc
     7381 gccgactcac attacctgtt tggcgaactt tatttcaaac cagctgctgt ttatccggct
     7441 ggtacgctaa tgaagattta cgaatgggat acagggaaat tccttggcga aattccccag
     7501 accttacaga catatagcgt ggtaggcaac atgaacgaat atcaggtagc aattggtgaa
     7561 accacctatg gcggacgtga ggagctacag gatagtactg ggttaattga ctacggttca
     7621 ttaatgtata tcgccctaca gcgctcaaag agcgcacgtg aggccattaa agtgatggct
     7681 gaactggttg aaaagtatgg ctactacagt tccggggaat cgttctcggt tgccgacaag
     7741 aacgaggttt ggatttttga aatgattgga aagggaaccg atattaaggt ggacaagaaa
     7801 actaaaaagg cttacaacgc aaatcgggga gcagtatggg tagccattcg tattcccgat
     7861 gggtacgtat cgggtcatgc taatcatgct cgtatcacca ccttccccct tgagaacatg
     7921 aaaacctcaa tcagctcaaa aaatttagat aaaatttttg aacccgatgt tgaggttgtt
     7981 tacgctcacg atgtaatcag ctttgctcgt caaaaaggtt actttaccgg taaagatgag
     8041 gaatttagct tttccgatgt atatgcccca ctcgattttg gtgcagctcg tttctgtgag
     8101 attagggttt ggtcgttttt caaggctgta aacaaggata tgtggaacta ctttgactac
     8161 gccaaggggc acgaccttaa aaaccgcatg cctttatata tcaagcctga gcgtaaactc
     8221 tcggttaggg atatgatgaa ctttatgcgc gatcaccttg agggaacaga actcgacatg
     8281 agtaaggatc caggcgcagg tcctcatggt ctcccatacc gttggcgtcc actaacctgg
     8341 aaatacgaag ggaaatcgta ctttaacgaa cgcgccactg caacccagca aaccggattc
     8401 tcgtttgttg cacaaatgcg cagctggcta cccgatgctg ttggcggtat tttctggttc
     8461 agtgttgacg atgctgcctc aaccgtttac ttccctgtat attgcagcgt tacctctgtg
     8521 ccagaggctt acgccgaggg caagggcgat atgctcacct actgcgacga ttgtgcattc
     8581 tgggtattca ataaggtatc gaactttgct tacctccgct acgaccttat gattcaggat
     8641 atcaaaaggg tacaggatga gttggaaaac cgtttcatct cacaaacagc cgtagttgac
     8701 caaaccgctc ttaagctatt ggaaaccaac aaggaggatg ctgtacgatt ccttaccgat
     8761 tataccgtta ataccggaaa ctatgttgtt gctcgctggg aaaaactatt ccagttcctt
     8821 ttaatgaaat ttatggatgg aaatgttaag caggaggatg gaaagggtgg gcttaaatac
     8881 aaccagtacg attactgccc tgccccagtt aaaaatccac aataccctga ctggtggaag
     8941 aaaaatgtga ttgatgcaac cggcgataag ctagctgttc ctgaaggtgc tgctagtcac
     9001 taaggcatca aacttaaagt aataaagagc aatcggctaa cgattgctct tttttatttg
     9061 gccaactcat tttatgggtt tatgtacaaa aaaacagcaa taccctaaat aaattttatg
     9121 accatgcagc aaatgataca tataaatcgt atttttagat tgttgaaaac caaattcaaa
     9181 tcaaatggaa aaaattgaga acagcccgaa aaccgactac tcaagactag tacttggatt
     9241 tttgctaatt gtgttggcag gtttactcct tatcgataac ttcgatttgt atcctgttga
     9301 atggggcaaa tacattttca cctggcaaac gttactaatt gtaataggat taattattct
     9361 gcccggtaaa gggaatagaa ctacaggaac tgttttaatt gccatagggg catttttcct
     9421 ggctgcaaag ttttacgaat tgcccgtttc aattagtaaa cttttttggc ctgcagtaat
     9481 tctttttata ggcctttcat taatatttgg tagcaggcga cgcaggtggc acccatacaa
     9541 aacaggtgaa gttaaggacg acttgattga tgatgtttcc atttttggtg gttcggacca
     9601 aaaatttact tcgcagcaat tcagaggggg aaaaattacc aacatttttg gaggctcaac
     9661 cattgacctt agcaccgcaa agctggaatc aggtttaaat gttgttgatt tgctatgcat
     9721 ttttggtggc tcaaaactca ttattccttc cgaatggaaa gtaaaaattg aggtaacctc
     9781 aatttttgga ggtgtttccg ataaacgaaa aatacctcag gccctaccag aaaatgcccc
     9841 agagcttgtt cttaaaggat tagtgctttt cggtggagta gatatcaaaa gttactaaga
     9901 tgaaataata atagttgaaa aggctgacag catcctgtcg gccttttttt tgtcaaaaaa
     9961 agtagctctc taacacatta aacaggcttg ttacttttac ataattcatt tacgtttgtg
    10021 taaattgtaa actgtaaaac tcaaaacgca ttaaaatgat taccaacgag ttaatcaatc
    10081 ccaaaagtat agtggtggtt ggcggttctg acgatattca gaaacccggc ggaaaggttt
    10141 taaaaaatct tatcgacaac cattttaaag ggagtcttta cgtagtaaac ccaaaagccg
    10201 atgaggttca gggagtgaaa tcggttcgcg atgttgctga attaccacag gtggatttgg
    10261 cgatactagc cattgcagct aagttctgcc ctcaaaccgt tgaggttctg gccacacaaa
    10321 aaggaaccag agctttcatt attctctcgg ccggttttca tgaagagagt gaggagggcg
    10381 ctaagcttga acaacaaata gttgacacca taaaccgtac cggtgggtgc ctaattggcc
    10441 caaactgcat tggtgtaatg aacactaact atgccggcgt ttttaccacc ccaattccca
    10501 ggttcgaccc caaaggagtt gattttattt cgggttcagg agccactgcg gtgtttatca
    10561 tggaatcgag cattccacgt ggcttaacat ttaacagcgt atactcagtg ggcaatagcg
    10621 cacaaattgg cgttgaggaa gtacttgaat acctcgataa tacattcgac ccccaaagca
    10681 gctcacgggt aaaactgctc tacgttgaaa gtatcaacaa acctctaaaa ctcttaaaac
    10741 atgcccagtc gctcataagg aaagggtgca ggattgcggc aattaaggct ggcagttcat
    10801 cggcaggaag ccgagcagca tcgagccaca ccggagcgct tgccagtagc gatgtagctg
    10861 ttgaagcgct gttccgcaaa gcgggaattg ttcgctgcta tgggcgcgat gagctggcta
    10921 ccgtggcatc aatttttatg catcccgagc ttcaagggaa aaacattgca gtaatcaccc
    10981 atgcaggtgg ccctgctgtt atgctcactg actctttatc gaacaacgga cttgaagtgc
    11041 ctccaattga ggggccaaag gcaaaggaac tgcttgccaa gctatttccg ggctcatcgg
    11101 tggctaaccc cattgacttt ttggcaacag gaacagccga gcagcttgga tttatcattg
    11161 atgcctgcga aaacgacttc cataatatcg atgccatgat agttattttt ggaagtcccg
    11221 gcctattccc cgtttacgat gtatacgacc tgcttgatga aaagatgaag gtttgtcgca
    11281 aacccatatt ccctgttctc ccctcggtta tgaacgtaaa gaatgagatt gaacacttta
    11341 ttgctaaagg acgaattttc ttccccgatg aggtatcgct tggcaacgcc cttgcaaaaa
    11401 tttaccatac accaaaacca tcacctgaag cacccgtttt accaaaggta aatgaaaaag
    11461 ccataagtga gattattagt aaaagcaata acggttacct gacacccgat gctgttcaac
    11521 agctgctcga tgctgccggt ataccccgtg ctggcgaagc ggttgttaca actgccgatg
    11581 atgctgccag agcagcagag aaattaggct atccggtagt aatgaaagta gttggccccg
    11641 tccacaaatc ggatgttggg ggtgttgtac ttaatgtgaa agattccgaa acggttcgca
    11701 aagagtttga ccgtatgatt aagattaagg atactacagc catccttatt cagccaatgc
    11761 ttaaaggggt tgaacttttt gttggggcaa aacgcgagga taaattcggt cacatggtac
    11821 tatgcggact tggaggtatt tttattgagg ttctaaagga tgtaaaagct gcccttgcac
    11881 ctattgatac aaacgaggca ttaaacatga taaaagggct taaaagctat ggaataatca
    11941 agggcgcaag agggcaggaa cctgttaatg aacagatttt tgccgaaatt gttagccgtt
    12001 tatcggcact cgtaacagtt gctcctgaaa tctttgagat ggacctaaac ccgcttctag
    12061 gttcaaaaga taaggttgtt gcggtagatg cccgaatcag aattgaaaaa tagtgcgcca
    12121 tgagccttaa ggagaaatgg aatagcctta cagctaacga aaaaatgatg attggccttg
    12181 ccattattct tgtaatactt attgcaacac gctttgagta tatatgggat caggttaagg
    12241 ctggttttgc tagatatttt aaccgttaaa tgttgattaa agtgaaacga gttgacttgt
    12301 acgtaatggt aggaactatt ttgttccttc tacccttttt tatatcggaa acggtttaca
    12361 acttttatgt tgattttaac gccagccatg gaatggttac aagtttcata aagtttgccc
    12421 tccttgccac cttcggcgaa gccattgggt taaggattag aacaggaaac tataatcaac
    12481 ccggatttgg attgttgccc agagcggttg tttggggctt gcttggttta accataaaat
    12541 tagcttttgt gctatttgca tcgggggttc cacaatttct tgcatatatg ggcatggaaa
    12601 atgcgcccca ggccttaaaa ggcgaactta ctttagccaa aatactcgta gccttcagca
    12661 ttagcctgtt tatgaacatt atttacgctc ctgtaatgat gacactccat aagattactg
    12721 acacccacat agtgaacaat ggtgggacac tttcgtgcct tttaaagccc atacattttg
    12781 gtaatattct tgcatcgata aattgggagg ttcaatggag ttttgttttt gccaaaacaa
    12841 taccctactt ctggatccca gctcatacca ttacatttct tttgcctgcc gattttcagg
    12901 ttctttttgc agctgtactg agcgtggcgc taggaatatt tcttgctgtg gcaagcctga
    12961 aaggtagtgg taaatagaaa tcgtagaaaa attatatatt tacatcaaaa gtaaagaatg
    13021 aaatatgaat aaacgtattg gaatatttta cggacccacc ggaggttcta ctgagaaggt
    13081 ggctcagctg atagccaaag aacttggagc gatagctgac ttacacccca taaaagaatc
    13141 aacgccttca atggtaaaca attacgacat tatcgttttt ggatgctcaa ccattggtag
    13201 cgaaacatgg aatggtgagg cttcgcgttc ggattgggac catttcagac cagagctgaa
    13261 aaaaatccaa gttcagggca agctctttgc cttttttgga accggcgatt cagttaccta
    13321 cccccgcaat tttgtcgacg gaatgggaat tcttgccaag gaattaattg ctcaaggggc
    13381 tcaggtgata ggccaatggc ctaccgatgg ctacagtttt accgactccg aagctctaat
    13441 taatggaaaa ttcattggct tacccattga tgaggattac gaggccgaca aaactattgc
    13501 ccgcgtaaaa ggttgggttg aactaataaa gaaatcaatc taggtattag gcattctaaa
    13561 aagaaagagc cacaatctgt ggctctttct tttctccctt ctacggcagt gaattcctaa
    13621 aactttggta tcggtcagca taggtattac cgaccttact gcctaaactt ctccacacat
    13681 catcaatgtt cttaatccta ttctcatccg atggatgagt gctaaggaat tcagggattt
    13741 tttgtgtaga acccatcatc ttctcaaaaa agccagcaag ttcgcgtggg tgataatcgg
    13801 tatcgcaaag gtaagtaaca gcatacttat cggcctcata ctcgttatcg cgactgtatt
    13861 tgagcattgt tccaccaagg gctaactgcg atagtatctg ctcaagctgc gaaggattgt
    13921 cacccataat aattgagagc aacatgctaa aaccatactg acgggtaagc atttgagtgc
    13981 tgtgacgcct atcggcatgg gctatttcat gcgccataac gccggctagc tgagcttcgt
    14041 tatccagaaa cttaattaat ccggtgtaaa aatataggta accaccaggt gccgcaaaag
    14101 cgtttaacac attgtcgtca tggatgattt ttacctgcca gggaaatgta gttgtatacc
    14161 tcagatcatc cgaacttaaa atggcattca ttaccctctc aatatgtgta tatgcctcag
    14221 cgtattgagt cctgtcaaga ataggatatt cattggggtt tgccaaaatt gcggaatcga
    14281 gctgcgcacc aaattgtata tcctgttcga gagtaaaaaa gtttatgcca tcgtcggaac
    14341 agctgcttag cagtatcaca ctcgttaaaa cccaaaataa tctcttcatt ttatcacttt
    14401 ttatcttgat ttaatttatt gaatttcaac tcaatacaca attcaatgca aatcaaatct
    14461 tttttgtcaa tgcaaattta atatattagc aaacattatc gataaaataa attattgatt
    14521 ggtagagcaa caagtcaaca attgatgacg gaagcaggaa acattgcaaa attaagtttt
    14581 aagaaattgt tagtaagagt aataacctac tcggtagtac tattctttac acttagtatt
    14641 agtttgactg tagtgtacaa atgggttaac ccacattaca catggttaat gatactaaaa
    14701 aagcatttac cggaaaccca aggaggaagt agcaattttg aacagagttg ggtaagtatc
    14761 gaagagatat cgccacagat ggtacttgct gtaattgcag ccgaagacaa taagttcatg
    14821 gaacacaacg gatttgactg ggaatcgata aaaaaggccc gaaagtataa tcaaagaggt
    14881 aaaaagttgc gcggagccag tacaatttcg cagcaaaccg caaaaaatgt atttctttgg
    14941 cccaaacgtt cctgggtacg aaaaggatta gaagcctact ttaccctgct cattgaaact
    15001 ttctggtcaa aggaaaggat aatggaagtt tacctgaacg tagccgagat gggaaggggt
    15061 atttacggag tggaagccgc agcaaggaaa tactacggta aacctgcatc gcgccttacc
    15121 cgtcatgagg ctgcaatgat tgccactacc ctacccgcac cagctaagcg aaatccatca
    15181 aaaccaagtg catacatggt gagctaccaa cgaaaaattc tttggaatat gtataattta
    15241 ggtgaaattg acattaatga aaaagaacag aaatagacac gataattaat actttaaaat
    15301 gcaaagatta ttattcccct tttggctaat tgtgactttt cttgtttgtt ttaccccttc
    15361 aaaggcccaa ttcaacaagg cgtacttttt ttacaaaggc gaggagttaa tttcaagagg
    15421 aaactatgct gatgccatac cagtactgaa caccctgcta aatgttgact caaccataca
    15481 cgaggcatgg tttctgcggg gcgtagcccg ctactaccaa aacgatttgg ttggtgcagg
    15541 aaacgatttt agtaagtgcc taaatattaa tcctttattt tcccaagcct accagtataa
    15601 tgcaatggtt ctgcagcaat tggggaaaag cgttgaatcc atagagtgta tccaaaaagc
    15661 catcgagctg cgaccagcat cggcgcaata cctttttacc tatggtgtta ttctattcca
    15721 gcaggaacag taccacgatg ctctttatgc cttcaaccag gtgcttaaaa ttgatgaccg
    15781 tatcccggat gcgtgggtta ataggggaac caccaggtta atgctctcgg atagttccgg
    15841 agcgttgacg gattattctg cggccataaa ccttaatccc tttaatgcaa acccttactt
    15901 acggaggggt gcgctttatg cccagcaatc aaactaccca cttgccttgc aagatcttaa
    15961 tcaagccatt gggattgata gcacactcgc acaggcatat tttacccgtg cactcgtttt
    16021 ctactaccaa aaacattata gccaggcatt atcagactta aataaggttt tgcagcttga
    16081 acaccgtaat acccttgccc tttttaacag agcgattata agctatgagg ttggcaaccc
    16141 cgaaagcgca attgatgacc taaccagact atcatcaatt attcctacca acgtgctggt
    16201 tcattactac ctggcctcaa tacgttatga actcggatat ttgcctatgg cactggagaa
    16261 tattaataaa gccatagcta tttttccaga gtttgccaat gcttaccggc ttagagccga
    16321 gattaaaacc aaaaaaggcg atttgaaagg tgccgaagct gacattgctc agggcaatag
    16381 attagcgcaa gcatacagta atagggcaat caatgatata gcctcagtaa tcgattccac
    16441 aggaaaacta aaacggttac tatcacttga cgaggaactc gacatcacct catcaattag
    16501 tatggaacag ctcagaaccc gaattctaac cattgtttac cctataatca ccctaaaagt
    16561 agataaaatc gaaaatgtaa agcctaggaa ctggcaaagt tcgaacctca ggcagattct
    16621 tgccacacag agttgccgcg gttacagcct attctttgaa tacgacacca ttaaccagca
    16681 ttcacaattg gaaaccatcg ccaccgattc attgtcggaa atttgtaaag cgatagccca
    16741 tttgcaaggc aactactata cggccgcaat gaaagaattt tcagaaacct taaaggctga
    16801 tagttcaaac actctttata ggcttgccga ggtgttggca aaaataaaaa tgacccaatt
    16861 tgttgaaaac gttaagaata aaagcttaca aagcgggaaa agtaatatat cccaaaaaac
    16921 aattgataac aaaccctaca ttactgctgg taatgatcta aaaactatag cattggacaa
    16981 cgactttgag ggcatagttc cttacaacat aggagtaatg tttatgcata ctgaaaataa
    17041 tgaccttgcc attgaatggt tttccaaagc tatcagcatc aaccccctta tacatggcgc
    17101 gtggtataac cgtggattac tctaccttat caataatgaa actgtaaaag ggtgtgccga
    17161 tctcagaaag gcagttaaca taggcaataa tcaggcctct gaggtggtag ctagattctg
    17221 taaaaagtaa aagccctgct aatttttatg cagggcttta ataacagcta atactcaaat
    17281 taaaagtata aatcgcgctc tccagctttg atacgctcaa ttcttcgacg aatctccagg
    17341 gtgttctgat gcccgtcgag ctctccaagg gttttttcaa taagcttgta gcctttctgc
    17401 tgaacctctg ggttggcata gtcaaccagg tactcagtaa gggttaacag ggcgtttggg
    17461 gtgcagtaac gctttataaa gccaggcact gagaactcca tgaaatgttc accggttctt
    17521 cccaggcggt agcaagccgt acagaacgaa gggatgtagt tatggtcaat aagctcatca
    17581 ataatttcac caagcgagcg gttatcgtta atctcaaatt gctccttatt aaggtcctga
    17641 tgttcattct tagatgcatg gtacgaaccc aactcaagct tagttccccc atcaatttgc
    17701 gatacaccaa actgaataac ctgcctacga atatcggccg gttcacgcgc tgtaagtatc
    17761 ataccggtgt atggcacagc caagcggagt attgcaacta actttacgaa atcgctatcg
    17821 gaaacaaaat acttatcggt gatatttaaa ccggatgcag acttgatgcg ggggaacgag
    17881 atggtatgcg gccctacatt atagcatgcc tcaaggtgat tggtatgcct aaccaggcca
    17941 agaacctcaa agcgccaatc gtagaggcca aagagtgccc caattccaac atcgtccaga
    18001 ccagcctcct gggcgcggtc gagcgaggtt aatcgccact caaaatcttt ctttttgccc
    18061 gaaggatggt accaggtata ggcctcaggg tgataggttt cctgaaagat ctggtaagtg
    18121 cctataccag cctcctttac cttccgaaat ccttcgatgt caagcggagc ggcattgata
    18181 ttaacccttc ggatctcacc gttgttcttc ttaattccat aaaccaacct aacggtgtgg
    18241 gcaatgtact cagccgagta ctctggatgt tcaccgtaaa caagtatcaa tcgcttttgc
    18301 ccgttatcct caagtgcttc aacttcctgt acaatctctt catcggaaag ggtcatacgt
    18361 atagcatcct tgttcgatgc cctaaaacca cagtactggc agttgtttgt acacttattc
    18421 cctatgtaaa gcggagcaaa cagtactatt ctgttcccgt aaatacgctc cttgagcgta
    18481 cgagcgccct gtagtatctc ctcaaccaat tcagggtcgg tggtattaac cagagtagca
    18541 acctcctcaa ggttaagcct attcttatcg agcgactttg caattatctc acgaaccctt
    18601 tccttggtgg gctttgcgtt gtttatgtat tcccatattt catcggcatc gatgaaaggc
    18661 ttcatgcgct catcgggaat tcggtacttt tccggattaa atttcatggc tctatatcgg
    18721 tttaaaatag ttaacactat caaacttacc tgagcctgat accagaggga aaagagattt
    18781 taaaaaaaaa ctacacccct ttgagcatta aggttttaac ctgaacaccg ctgagcttac
    18841 caatgggccc ggtaagggct ccaatttcat cggttgttcc ttcaagcaca agcgatatta
    18901 cgctaatacc cctctgctgg agcggtatgc cctgacgggc aataataata tcggcatggg
    18961 ccgaaaggat ctggttcagc cttgaggcgc tctcgcggtt ctcaacaaga attagtgcag
    19021 ctccgattct cttttccatc agactatctc ctttggatca gactcatttt ctgaatcctt
    19081 ggtggcaaac atctaccaac ctcagatgca ggaattacat ttcaaaaaac ctaaatactt
    19141 atacgattaa ctatgcccca aagatacgtt aaaatacaat tggttaacaa tgataaatat
    19201 cattactgtt aataaattaa ctcatcaacc tgcttggctg cctgtgtaag ctgctggaat
    19261 aacaacctgt tatcggagat atcaattcta tcagctatct gcttatgaaa gtaaatatag
    19321 gcatcgctac tgtgaagcat accatcaaaa ttgaggatat gaagttcaac attccttgtc
    19381 aaaactgggt tcaaatcggt attatcgcca tttgaccaaa tgataaccat ggcacgatac
    19441 gatgatttat tccattccag cgaaacggca taaatgccct ccggtggaat gagcttgcct
    19501 gcctcctcaa tttgaaccgt tagggttggg aaacccacct cgccaaaaaa gtgcgaaccc
    19561 ttacccaaag caccaataat catgtagtaa tggtcaaggt aagcatttgc ccgttgtatt
    19621 tttccctcaa gtaaagcctt acgaatagtg gtggaactaa ccgtttcctg ctgtatatcc
    19681 tgctcgggta tctcctcaac cataaaatta tactccgaac tcaatttttt gagctcctca
    19741 taatcgcctt cgcggttgtg tccaaaatga tgattgaaac caactacgat gtatcgagcg
    19801 ttcagcttac taatcaagta atccctgata aattgaaccg atgaaatttt tgaaaattcg
    19861 agggtaaatt ttactatgat tagatgatcg aggccggttt tgctaagcag ctcaattttt
    19921 tcgcgttgcg acaggataaa catcaggttt ttaccagcag tttcagggta aagcactttg
    19981 cgcggatgag ggtaaaaggt aataagaacc gactcgccgc ctatgctttg agcaatctca
    20041 tttatcctgt taataattac cttatggcct aagtgaaccc catcaaacga cccagtggtg
    20101 accaccggat tagttatttc ccctatacca tcaaaaccat aatgtacttt cattactatc
    20161 gtttaccggg aatagccctg tttaacaaag taagccactt tctccatgga ggtaatcata
    20221 tcgtactcct caaggtaaac attctcaggt acaactaaag gaattgtggt gaagttaagg
    20281 atacccttta taccagctgc aacaagttgc tcggcaactt gattggcagt atcgggtggc
    20341 gatgagataa tggcaatgga aatatcgtta cgttttataa catcgggcaa ctccttcatg
    20401 tggtagcaat ttacaccaga aattaccctg ttaaccttgt tgggatctac atcaaaagcg
    20461 gcaactatat taagtttagg gcgtttgcca ttgaaatagg tagtgatagc gcgtcctagg
    20521 ttacccatac caacaacagc tacatttaat cctactttgt ggtcgatgat atcgccaata
    20581 accttgataa gttcctgtgc atcgtaccct ttctttttca tgctagagta gccaataagc
    20641 atcaggtcgc gccttacctg aaccgctgtg atattatgca aaccggcgag ctcatgtgag
    20701 tagatatgag ttttccctcg tgttaagcaa ttcagaaagg ttctgcgata tttgctcaat
    20761 cgctcaatgg ttttttcggg taggtttgcc ttatccatag ctcaactatt acatttttca
    20821 acacaaaact atttaacttt tttcagttaa caaacggctg tttaaaataa agtggattta
    20881 aataccgtcg ttaagggtat cggaaccggg taaagggaat gtaatggtaa aggttgatcc
    20941 tgcaccaggc tcgctttgca catcaattct agcgccatac aaatccacta ctcgcttaac
    21001 aattgaaagt cctaagccgc ttccggttac atttttgctc tgcggatttt tgatgcgagt
    21061 aaactcctgg aaaagtttag gtatatcctc gggttcgatg ccaattcctg aatcggatac
    21121 aactagcgta acctctgatg ctgttttgct gatactacac ttaacccacc caccgggttt
    21181 attgtatttc acggcattgg aaataaggtt gttgaacaaa atttccatgt cgtcggaatc
    21241 ggcaagcatg ataacgggtt ctgtggtttg caggctaagg ttgacgtcgc gctgaatggc
    21301 aaggggagtc atggtctcaa tggccgaacg ggccacgagg gttaaatcga tttcacgaag
    21361 ggttcgctgc cgtttgcctg actcaatgtg cgtgaggtcg agtaggtcca ttattagatt
    21421 acgcatggac tggattcgtg caagcgaacg gtcgataaaa tccatgtaac tgtcaatact
    21481 ctcgccagcc tgcttctcct gcatcatttt aaggtagccc tcaacggcgt taagaggtgc
    21541 ttttagctcg tgcgagagca cggtaaggaa ctgaaaccga acctgcttgc cctcacgatg
    21601 gagcttgcgg gtcatacgcc gcaggaaata atgcttggtg atattctcaa tggacgactt
    21661 aagctcctgt ggagtaaagg gcttgggaac aaaatcgtag gtaccaatgt ttgtagccct
    21721 aacagccaac tccagcgaag catacgatgt aatcatcatt accagtatat cggggtgatt
    21781 ctggttgatg aactgcaaaa cctcaacccc ctgaatgcct ggtagtttat tatcgagtaa
    21841 tacaatggct ggactcgaac gcttgataat atcgattgcc tcctcccctg tggccgcttc
    21901 aataacatta aagcctatat catcctcaag gaatgggtag ctaaccgtga agttcctaag
    21961 aatacgtgtt acaccggaac gaattcccgg ttcatcgtca acaacgagta tatcaacaac
    22021 ttccattttt acaccttaag caatttgaga atttcacgat gcagctggtc ggggcgaatt
    22081 cccttttcga ggtataaatc agccttaatc cactggcgtt cctgttcgct tgaaatgcca
    22141 aacgacatgc ctgtctcagc cgatacagca gtggctatta ttacgggaac atcgggatac
    22201 attcgtttta gcttgtagct aagaataaaa ccgctatcct cgttctccat cattaggtca
    22261 agtatggcta aatcgggttt gatattcttt aggatattct ccgcctcacg ctgactttca
    22321 gcggtaacca catcgaagcc cattttttca acatgaaact taagctgaaa caggtagtcg
    22381 gtatcatcgt caactattag aatggttttt cgtgaattag ccatagctat tgcgtttagt
    22441 taactattat cgtttcctaa ttcgttttcc gcattacggg gcagggttat cctaaaggtt
    22501 gttccggtag gtcccttttg ggggtcggtg ttggactcaa catctatttt gcccttgtgc
    22561 atctttacaa ttccgtaggt agtggcaagc cctaaaccgg ttcccttgcc aattccctta
    22621 gtggtgaaaa atggggtgaa gagcttgtcc atattctcct cgggtatccc ttcgccagta
    22681 tcgattacgc tgattgaaat ctgatggtca tcgccgtcga taacaattgt aaggtcgcca
    22741 ccattaggca tggcttcaaa ggcatttttg ttcaggtttg taagcacctg catcatttgc
    22801 tcgtaatcga gactcgccat caggtttgtg gttcggcaaa ccacattggt ggtaacattc
    22861 tttgggataa caacccctgc aatgctatcc tcagccaact tcttaacatt aaccttttcc
    22921 accttaacct gattcttgcg ggcaaagttt agcaaaccgc taacaatttt cttgcaccgt
    22981 tccgcctgcg acgatatcaa ctctaagtcc ttacgaaggg gatcgtccgg tttacactca
    23041 tcgagcagga tattgctgta catcaaaaca acacctaaag gattgttaag ctcgtgagca
    23101 ataccggccg aaagctgccc catatgggcg agcttttcgg aatgcttgag agcctgctgc
    23161 atttttgcca gtttctcatt ggaaatggct agatcgttga ttgactcatg cagttcctca
    23221 atagcgtaag gtaaacacat ttcaacctcg gcaaggcctt caacaatagc tatagcgtgc
    23281 tcggtgcaag tatcgtaacc acatgcgccg cagttcaggt gatcgcgagg ggtaaactta
    23341 cccatctttg ccaacacttt gtttatctca tcatcggtag gcttagctat tcgtttatca
    23401 tcgggattaa agctacgcgt aaggtcaaga tctttaaatt ttgatatggc tagctcccac
    23461 tcttttagct gctcaggggt taccttatta cgtgcatagg aactgatata ggttttgcgc
    23521 tcaaaacgtt tgcctccggg cgacatgccg ggccccatta tgcaaccctc acagcaaagt
    23581 agttcaaggt tttgtccctt aaggttaccc gattcccatt cctttatggc ttcctgaaag
    23641 cctattcgtc cttcagctac tgtgatgttg ccttcaatta ggtcatcaaa tatgttaacg
    23701 gtttgcatta accccctact aaccgggaat atcgccccac ggccagcatg gggtggatca
    23761 aaatccgatg gttcaacgct ttcgggcgtt atactggcct gctcaaaaag aacccgcaac
    23821 tccctaaagg ttattgcctc gtcaatctca atggattcat cctttttggc tatgcaagga
    23881 ccaataaaaa cctgtttcag gttactgtca atttgcctta ccaccctgct tatggccacc
    23941 ataggcgaca ccacgggtgc taaattttct actaaatcgg gatggtagta acggatgtag
    24001 gtaacaatag ccggacaatc gcttgagata taaccctttc ctgacctatc ctccagcaac
    24061 gatttgtaac ggctagctac caaatcggca ccaaaagcaa cctcaaaaac atgactaaac
    24121 cccaaggccc tgagcattcc aacaaagtag cgataatcat caatctcggt aaattcagcg
    24181 ggaaaacttg gagctacaag agcagcaacc ctttcgccgc tctgcagcat gctttttacc
    24241 tcggtgcggg ttagtaaaaa cacctttgca tcctgactgc ataccttaac gcagttgcca
    24301 caaccaatgc acctttccga tattacatcg gcttggccat ttacaatttt gattgcctta
    24361 gccggacact ccctaacaca ggtataacac accctgcata actccttaat ggtgtatacc
    24421 aatttcctct tttcccgatt catctccatg ctatagccga attgttccat ttaaagtaaa
    24481 acttcccgct ttgaatatcg ggtgtataga aatttcttgg ctttttcact acgggtttcc
    24541 cctaaaaact catcgtatat gtattgtacg gcagggttac gggttgggca cttaattcct
    24601 tccgcctcat caatttcaat aattgctttg gccctcaact taacatactt ttcctcgtca
    24661 acatagggtt ggcctcctcc atttacacaa ccaccgtggc aagccattac ctcgatgtag
    24721 tccagtttaa catcgcttcc catcaaaacc gggagaatag aatgtatgtt ttttaaccca
    24781 ttaacaactg caaaggtgaa tgtttgccca gcggcattga aagagaatgc ctttacccca
    24841 gatagtattt taacctcgtt gaccttacta tcggtaagct cttttgaggt tagtttatgg
    24901 taaaccaccc gggcaagcgc ctcggctgaa cctcctgata ttccaaatag ctttccggac
    24961 gagctacgaa tattaaaagg tttatcggga tgttgcgatt caatcccctg aatatcgata
    25021 ccgtaaagtt taatcagcct aaccaattca cgtgttgata ataccgtatc cacatcagat
    25081 aggccacggg tagtcatctc ctccctttga gcttcaaact ttttggccaa acaaggcatt
    25141 acggaaacgg agaaaatttt tgatggattt aattcatttt cggcagcaaa gtagttcctt
    25201 attaacgatc ccattacctg ctgaggcgat ttaacggcac tcagtgccgg aatataatcc
    25261 ggataccact gttcaacaaa cttaacccat gccgggcagc atgatgagaa catggggaga
    25321 ttactgccat ttccctttcg ttcaatgagc tcagctgcca tttcaattgc agcgacatcg
    25381 gcagcaaaag cagtatcgaa aacatacttg aacccaattt ttcgcaatgc attgtacagc
    25441 aaaccattaa catccttgct gggtttaagc ccgaattcag tagctaccga aaccgaaatt
    25501 gaaggggcaa tctgcacaac agggattaaa tcaggattgc ttagggcatc cattacttcg
    25561 gcaacatttt gacgctcatg taaagcgcca gtagggcaaa ccaatacaca ctgtccgcaa
    25621 ccaatacagt ttgagaagtt taaatcgcgc gacaaagtgg tacccacata catgtcgcat
    25681 cccctgttga caaactcaaa ggttgatacc gattgaacct catcgcaaac ccttatacat
    25741 cttccacaaa gaatacactt agccggatcg cggacaatac ttgggctgga aagatcaagc
    25801 ttttgagtaa acgactttcg taaaacctta cgctcacgaa tgtgtagctc tgctgctaaa
    25861 tcctgtagct cgcaaaagcc gttccgttca cagtataagc agtcgtcggg atggttggca
    25921 aggagcaact caaccagggt tttacgggcg cgcaccactc ggggtgaatg ggtcataatt
    25981 cgcataccat tctccaccac atgcgagcat gaggtaacaa ggttatctcg accctcaacc
    26041 tcaacaacgc atagcctaca tgcccctgat ggggttaaat cggtcatgtt gcaaagagtg
    26101 ggcaccctga taccgttacg gtttaaggcc tcaagaatgg tatcgccttt atgggcttgt
    26161 attgttctat tatttacttc gatggtaatg ttcatagcag caaaaacttt acttgaagaa
    26221 aacagctcca aacttgcatg tatcctggca tataccgcat cctatacaac gctcctccac
    26281 aataaagtag ggagaacgtg gtgtcccata tatggcctgt gttggacact tcttggcaca
    26341 ggcagtgcaa cccgtacaac gctcaatatc gatgtaaaat gtccttaaat cgcgacaaac
    26401 gcttgccctg cacttccgat caaagatatg ctcctcaaac tcatcgcgga acgattttag
    26461 agccttaagg attggtttgg gtgccgtttg ccccaatccg catagcgatg tgtcgcgcat
    26521 aacctcagcc agggtttcga gctgaataac acccttaaac cgttcaaggg tattatgccc
    26581 ttcgttacta actggtcgtt tcgatatgtt atcgagtatt tggtacatcc ggtttgatcc
    26641 ttcacggcaa ggaatacatt taccgcagct ctcgttacga ataaagtgca tgaagtattt
    26701 caccaggtca accatgcaca cggtattgtc aaaaacctga acaccacccg atcctaaact
    26761 tatccctcgt ttcggaagtt catcaaagtc aataggggca tcaagttcat cggctgtaac
    26821 actcacccca gaagggccgc ccaggtgcaa tgccttgaaa gtccttccgt cctttacccc
    26881 tcctgcaagg tcaagcaaag ccccaagggg ttcaccaaag gttaactcaa ccatacaggt
    26941 ttgagacgcc tgaccattaa tggagaaaat tttcgttccc tttgacccct cagttcctaa
    27001 agatgcaaac cactgaggcc cattactgat aatttcgggg atattggcta aagtctcaac
    27061 attattgata acagtgggct ttccatttaa tccaaattca gcagggtaag gcggttttat
    27121 gctgggcatt ccacgtttac cctcaaggct tttaatcaag gcagtttcct caccgcatac
    27181 gtaagccccg gggcctttac gcacctctat atcgagcgaa tagccactcc ctaaaatatc
    27241 atatccaagt agtcccacct catatgcctg gtcaatagca ttctgaattc tttgaatggt
    27301 aatcttatac ctatcgcgta tgtaaataat tgctttactt gccgacacgg catacgagct
    27361 caaaataatt ccttcaacaa ccctatgcgg atcgctttca atcagcagtc ggtccatgaa
    27421 tccccccggg tcactctcgt cggcattgca aatcaggtat cgatcgctgg ttgcaacctg
    27481 taacgctttg cgccacttgg tgcctgtggg gaaggctcca ccgccccttc cgcgtaatcc
    27541 gctctcctca actatgcggc aaatatcgtc gtaggtgtac ttcctgatag acttagccaa
    27601 agcgctatat ccgccatggg caatatactc ggcaatgcta tcggggcaaa tgatacctgc
    27661 atttttaagc aacacacggt tttgcctttt aaaaaaggga agttcgtttc taaaaggaat
    27721 tccatgccaa ggctcatgaa tctcggaata gtactggaaa atggcattct caggctgaat
    27781 aaaaccatta agagctccat cgagtatgga gttaaccctt tcaggagtta cattggcaaa
    27841 agccactcta gtcctaccgg gcatctgtac ttcaacaatg ggctcaagag agcataaacc
    27901 atggctacca acacatatta catcggcatc aacagaataa tcgtctaagt actgctcaat
    27961 agctgaccta gttttaagcg ctcctgcaac aatggacgat gtagttacgc caacatatat
    28021 tattggctgc ctaacccgtt catgccttaa tgcgctaagc ttagcgttta cctcggtaag
    28081 ccatacccca tcgtgaaggt ttggaataac agcatctttt ataaaagata tttcagcttt
    28141 tgaatcgcac cgagccatgt ttactcttgg gttaaatcgt tcaaaatatt tttaagcttt
    28201 tcggggtcaa cctcacggta atactcatcg ttgacggaaa tcactgggcc atgttcacat
    28261 gcacccatgc aggttaccac ctcaaggcta aactgcccat tccgggttgt ttgaccattt
    28321 ttcagcttca aattatcctc aaggtaaacc atattccact tggcaccctt tagcctacac
    28381 gaggtgccat tacaaaggca gatatgattt ttccccttag cttcaaactt aaactggtta
    28441 tagaatgtgg ctaagccata aattttggaa gttggcatgt ttaactttcg ccctaccaga
    28501 acaattgctt cctcgttcag acttcccaac tcgttttgaa tatcctgtag aattgggata
    28561 aggctatcgg aacctaagta gctgtagcgt tctagaatct cctcaatttt ctcggcgttt
    28621 gacatagctt taaagtaagg tagtaattag aaccatagtt aacccaccat tctaattcat
    28681 ctgttaaata actaacagag acatatattt ttagtaatca attagttaca acaaataaaa
    28741 tcttgttaat cacggaacaa tttaataaaa cattacaaaa attgttcgcc aaacaccaat
    28801 attttatcta tttaaaacga ttacagataa gtgatttgat ttaaaaaatg attaaatttc
    28861 aggcataatt tgtaaaccaa aacctatctt agcattttgt aatagtgtta taccgataac
    28921 attaagatga gtaaacgagc cgaattaatt atatgtttgg gtagttcctg ctttgcaagg
    28981 ggtaataagg ccacactcaa ggcaataatg aaatttttgg acgatcacga tttgaaagac
    29041 aaggtaaact tccacggggg acattgtttt ggtaattgcg ccgaaggtcc taacatctct
    29101 attaacggta aaatttatac cggggttgat gaaaatggaa ttattgacat tcttacaaat
    29161 gagctacttt aaggagacat atgtcacaac tgtttagaat agacgagcaa acctgcatta
    29221 actgctacgc atgcgttagg gtttgtcctg taaaagccat tgaggtgaag gcaggccaaa
    29281 actacgctcg aataattgat agccgatgtg taggatgtgg gagctgttta aatatttgtc
    29341 cggttaatgc cgtaagctat agatcctcaa aaaaggaggt tttggagctg ctaaaaggcg
    29401 acgaacccgt tgcggctatt tgttccccaa gtatttcggg tgaatttgtg gatattaccg
    29461 actatcgtaa gtttgtacag atgattaagc agctaggttt tacctacgtt tgcgaggtaa
    29521 cctttggggt tgacctggtt gcgcgggaat acaaaaagct tttcgaagat tttaaaggca
    29581 agtactttat taccagcatg tgtccggtgg tggtgtcgat ggttgagaaa taccaccccg
    29641 agattgctca aaaccttgcc cccataatct ccccagttgt ggctacagcc atggcggtca
    29701 ggaaagagta tggctctaac gttagagtag ttcttattgg cccatgcatc caaagcaaag
    29761 aagaaattag actctacgac gacgaacgaa gaattgatga aaacctcaca tttgttgagc
    29821 tcagggaact ttttgatgag tttaacatca aggagagcaa tttggaatac tctgaatttg
    29881 acgagccaat tggatataag ggttcattgt tcccaattgg gcgaggtata ctgcaagctg
    29941 tagatattag cgaggatcta ctcaccggta cagtaatcag caccaatggt ccaaacaaca
    30001 ccttacaagc ccttgagcaa tttgaaacca gcatcgatct gataaacagg catttcaatc
    30061 ttttctactg tgaaggttgc ttgatggggc ctggaacctc acgaggtgga aaaaagttca
    30121 tcaggcgaac aatggtggtt aactacgcta acaagcgatt aaaaaccttt gatcgcgaaa
    30181 aatgggaaaa aaatatcgca aaaaatagtc ttgggaattt cgcaaggggt ttcacaattg
    30241 atgaccaacg ccttcctaca cccaacgagc ataaacttga agaggtttta aaggtgattg
    30301 ataaagagtt tgttgggcgc gattcggggt gcgaggcatg cggatactcc tcgtgctacg
    30361 attttgcctc ggctgtagca tcggggcttg cccatactga tatgtgcttg aactttaccc
    30421 ttaaaaagcg ccaggaatac ataaaaaccc ttcaggccac taatgaaaaa ctcgctaaaa
    30481 ctcaggaagc ccttaaggag tcagaaaaga aagctagaac cgagcaagag gcagcccggg
    30541 aagctactga aaccattacc gccatgctta aaaaactgcc ttcggcagta gtgcttatcg
    30601 acaaaaaaat gaaaatcctc cagagtaacc aaagttttat tgatctgttg ggcgacgatg
    30661 ctttggagat taatgagata atccctggac ttgttggtgc cgaccttaaa accctgctcc
    30721 catacaacat ccacaacctt tttaccagcg tgctccaaaa taacactgat atcctgaacc
    30781 gcgatgtgat gtacaaggat aagctgctta acctttcagt attcaccatt aaacccaata
    30841 gcattgttgg ggctgttatc cgcgatatgt atgcccccga ggttcagaag gaggaggtta
    30901 ttaaacggat taccgaagtt attgataaaa acctagccat ggtacaaaac atcggattcc
    30961 tgctgggcga aggtgcatcg gaaaccgaaa agatgctcaa ctcaatcatt aaaacatacc
    31021 gcgagggaaa aaataacgac gataaagcat agtagcctaa aaccatgtcg agtaagtttt
    31081 tcatagaggt aaactgccaa caaaagaatc acgacgagga gcgtatttgt ggcgacgttt
    31141 tttactcgcg caggttacag gacgaagagc gcaccataat tgtgctatcc gatggcatgg
    31201 ggcatggtgt taaggcaaat gtactggcca ccctcacatc cactatggca cttaacttca
    31261 ccatagagca taaggaggtt aacactattg ctgaaattat tatgaatacc cttccggttt
    31321 gctcagtccg caaaatgagt tactctacat tttccattgt tgatattgac cacaacggcg
    31381 aaactcgcat actggagtac gacaaccctc aatgcctgat catgcgggga accgaggaac
    31441 ttgaaccaga gtggcaatgc ataatcctta acagcgaaaa aaatgcgggt aaagagttaa
    31501 agttctgctc gtttgttccg caaaaggaag accgaataat cctttggacc gatggagttg
    31561 ctcaatccgg actgggctca gccgattacc ctttcggctg gggcgacgag gcacgcgaat
    31621 ttgtgaaacg aatggttaaa aatgaacccg atatttcggc ccggaagcta agcaccaagg
    31681 tggtaaatat ggccagcctc aacgatggat atcatcccaa ggat
//
LOCUS       DAOOWJ010000021        31709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1447_length_31709_cov_7.841031, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000021 DAOOWJ010000000
VERSION     DAOOWJ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 31709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31709
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1447_length_31709_cov_7.841031"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(529..1674)
                     /locus_tag="PLW22_03680"
     CDS             complement(529..1674)
                     /locus_tag="PLW22_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU73838.1"
                     /translation="MKANFVILSSLILLSFRLSAQVVFVVDSFPARTRPDDTIFMASE
                     LNGWNPHDISWAFKRTTNRYELKLTHVKDTFEYKLSRGSWQLVEVDSNGGDIRNRIYF
                     PPTDTVSIKVNGWRDMFEAAQRKSTASTNVRFLPSTLEMKQLNRRRAVRLYLPPNYSK
                     RQQFPVIYMHDGQNVFDEATSFAGEWRVDEIMDSLYYSCGFAAIVVAIYNDSKERINE
                     YSPWRNDSLGMGGDGDKYAYFVAKELKPFIDKNYRTNPEPKFNAVIGSSMGGLISLYI
                     GLKYPDAFGRVGVFSPSLWFSPKVFEYVSKYKRRADQKIYLLAGEKESETLVEDMKML
                     EHHLYKAGYKDEDYVKMKIAPDGRHSEWFWSREFTEALEYLFEIRKN"
     gene            1765..1838
                     /locus_tag="PLW22_03685"
     tRNA            1765..1838
                     /locus_tag="PLW22_03685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1799..1801,aa:Arg,seq:ccg)
     gene            2134..3381
                     /locus_tag="PLW22_03690"
     CDS             2134..3381
                     /locus_tag="PLW22_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73839.1"
                     /translation="MENSIRNNLDNPEVLEQLYRKNRKGFEKAFFSIYSEISSIPLAK
                     FWFTRLTADNKILFNVNLKEIAYVLISCLIVSIVMLIPWLFGFNPHENNFYERNLALT
                     ALLGVTLYTAILKNINLKKTAAIVGFFIISAIYVNVLPKTESSSTTLIYIHLPILLWF
                     IFGIVFSEFELKNNEKRIEFIKYNGDLAVLFSIFAIAGMILTAFTIGLFSAIGINVEK
                     IYFELIVIPGVVSVPIICTYLINKFEYLSNKIVTIVANIFSPLILITLIIYLISIVAK
                     GSNPFVNRDFLIIFNAMLIGVVALVIFSISERVNNSTNNYYTIIFTALSTIALLINVV
                     ALLAILYRITEYGITPNRLAVIGSNLLFFTHLILILTNLLRVIRKKQHLTIIEQVTAK
                     FLPYYGIWLIFVVFILPFIFNFK"
     gene            3474..4106
                     /locus_tag="PLW22_03695"
     CDS             3474..4106
                     /locus_tag="PLW22_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73840.1"
                     /translation="MKRVVISSLLLFATTGLFAQIFVGGSVGFNTTGGKIENGNTSVD
                     KVTQTSFSLAPKAGMFLSEKLAVGAILGFNLQSEKTPGTPEEIDRTTTFGLTPFARYY
                     AFSLNKFSVFAQGNLGFSYGVEKHKEGSTTTTGPKTTTIGISAFPGISYKLTDKVELE
                     AVIGGLNLNFNRVSVKNNNTTNITNTFGVGANLDAIATTGFITIGAIVKL"
     gene            complement(4150..4809)
                     /gene="nth"
                     /locus_tag="PLW22_03700"
     CDS             complement(4150..4809)
                     /gene="nth"
                     /locus_tag="PLW22_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU73841.1"
                     /translation="MMNSKKELYRFVIEYFEKTRPIAETELHYSNPYELLVAVILSAQ
                     CTDKRVNLITPNFFKSFPDAVSLANASVEDIYKIIKSCSYPNSKAKNLLGMAKVLMTE
                     FNGEVPSDINLLQRLPGVGRKTANVIASVVFDKPAMAVDTHVFRVANRIGLVSNAKTP
                     LAVERQLMENFPLELIPKAHHWLILHGRYVCTARKPKCTECGLTLVCKEYKKISKKAE
                     K"
     gene            5146..7023
                     /locus_tag="PLW22_03705"
     CDS             5146..7023
                     /locus_tag="PLW22_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU73842.1"
                     /translation="MALIYLQAENVSKSFGDLEVLSDVSLSLFQNQRTALVARNGTGK
                     STLLNILAGYDTPDSGKVTLRNDISVGYLPQEPQLNLSKSVLENVFNADDAISVAIRN
                     YEKAIESTDKMALQNAIDEMDRLNIWDREQKAKQILSVFQLTDINQPVQLLSGGQRKR
                     VALAAVLISNPDLLILDEPTNHLDLEMVEWLEDYLTRSQKTVLMVTHDRYFLDRVCTD
                     IIEIDQKQVYTYNGNYSYFIEKRDERIRAKLAEIDRASNLLRRETEWMRRMPQARGTK
                     AKYRIDAFYKLRETALQKIHSNDVEIGVSASRLGKKIMEVHNLSKSFDSKVILKDFSY
                     TFNRFERLGIVGANGTGKTTFLNLITGTLPPDSGTIDPGATLKIAYYRQSGMNIPEDV
                     KVIDVVREIAEVITLANGKTITASQFLNNFLFTPDRQHTLVRKLSGGEKRRLYLLTVL
                     MQNPNFLILDEPTNDLDIFTLNVLEDYLINFPGVVVVVSHDRYFMDKLVDGLLIFEGN
                     GEISGFPGNYTEYREWAKEQEKSNAEQQTSSKGKTDSKRPQPERKKSKLTFAQKKELE
                     KLTDEIVKLEKEKKEIEGLLNGGIQDAKLLMDISNRFSEISKLLEDKEMRWLELNEFE
                     E"
     gene            complement(7032..8360)
                     /gene="tilS"
                     /locus_tag="PLW22_03710"
     CDS             complement(7032..8360)
                     /gene="tilS"
                     /locus_tag="PLW22_03710"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOU73843.1"
                     /translation="MLQSKMIDFVREHQMANDQDKILVGVSGGIDSMVLLHLLVHAGY
                     SVGVAHCNFSLRGDESDADEQFVRYHCLMNGLPVHTIKFDTLEYAKENGLSIQVAARE
                     LRYAYFDEICKEHKYTRIAIAHNLNDSVETVLLNLSRGTGLRGLTGIKPVNGNIIRPL
                     LFATRVQVQEYATLHNISYREDSSNASDKYKRNYIRHNVLPLLRELNPSVDISIYQTA
                     QHLREAQLIVDSFLTQLRKEIVNENGGVFYIDIEKLKNQAAVNIFLVDFLSDFNFTPA
                     LTTEVCELLESSIGKRVESDTHVIFRDRNHLILQQKQKRQSVECQIDVNITQIDQPVK
                     LSLSKLDYSQGFVFGKDTKYAYLDLEKLKFPLTIRPWQPGDRFMPFGMTSFKKISDFL
                     VDIKIPLPDKEKVFVLTSENQIVWVVGYRIDNRFRVDENTQKILLIKFDE"
     gene            complement(8347..9669)
                     /locus_tag="PLW22_03715"
     CDS             complement(8347..9669)
                     /locus_tag="PLW22_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOU73844.1"
                     /translation="MRSKYIHRVSNFDRFAKNLAYEVENTDVGILLRGSDRFTIPETN
                     FTSRFQLAAALGISSACSPNSNHFDELDEFLSAHNDWVFGYFTYDLKNQLEHLQSNNP
                     DGVGLPDMFFFVPRILILVNGEIAEFHYLQQEVSQEEVSDLIDRLEGDLEELDHNVSV
                     VFRSHYSYNDYLNTVSAVKQHIARGDVYELNLCQEFFANNVRITPYVVFNKLYDRSPT
                     PFGAFLRVNDKFLMCASPERYLRRDGDWLFSQPIKGTAPRMESDESDQQEIFRLKTDP
                     KERAENVMIVDLVRNDLSRIAKRGSVRVNELCGIYTFPQVHQMISTVSCELADGLRPT
                     DPFRATFPMGSMTGAPKVRVMELIENFERSKRGLYSGAVGYFMPNGNFDFNVVIRSLL
                     YNRSNGYLSFTVGGAITFKSQPEAEYRECLVKAKAILETLNAQIDAAE"
     gene            complement(9716..10738)
                     /locus_tag="PLW22_03720"
     CDS             complement(9716..10738)
                     /locus_tag="PLW22_03720"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HOU73845.1"
                     /translation="MTKTILVTGGTGYIGSWVVKELLEKGYRVRVPVRSFNRKDKYLH
                     LQNLPNSTNLLELFEADLLVDGAFDEAAEGTDAIIHMASPFTLRFKDPQRDLVDPAVK
                     GTRNVLGAASKSSTVKRVVLTSSVAAVHGDNIDMEEQGLTEFTEENYNTTSSLKHQPY
                     SFSKVQAEREAWRINAEQSKWELVVINPALVLGPSLAGNSNSESLQIMKDLLSGKYRF
                     GAPDLYFGFVDVRDVAKAHVLALENPSVSGRFIIAERVANFMELVAIINNKFPGQFKL
                     PKMVAPKWLLYFVSPLFGLSPRFVARNVGYRIAFSTSRSRQELGLGYTPFEHTVVDMV
                     NQFKKN"
     gene            complement(10802..12640)
                     /locus_tag="PLW22_03725"
     CDS             complement(10802..12640)
                     /locus_tag="PLW22_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73846.1"
                     /translation="MTDFRLLSYVRIKTIIALVMMVYFSFFGFINYSSFAQSDSNQVS
                     SDSIKKKDDKISQTVYKWFGRQNVNVAPLTNEETVNRFLKYEGKVISQISIVRFDAFG
                     YSVYDTTIKPERSIEKFGNSLHLKTWKKIIQNNLLFKEGDKVVPLDLAETEQLLRQSN
                     FFEDANILVQLLSDSNHVHVYVITKDIWSMSVGVQYSSLEKMKLALSERNFAGLGIRA
                     KAIAYYNKTFTDKWSYQGEFDVPNYFGSYISSSFFVRKGHSYETYLMRFNRDFFASKT
                     KYAGGVELLKSDEPYRVFTDDSVVQISYQSQDWWIGRSVRVSRRSGTSAPLNLVFALR
                     YYRRDYFQSPPVSIYLNPIFHSAENYVVSLGLSNQNIYRSSLYFGFGNTEDIPVGFKI
                     QATSGIQQSTFQRRFLIGGELSAAEVTPWGYLFLSSRLGGYLAEGPKVEQAVINLRAQ
                     YFSNLYHIGRSDIRHFIRYDFTRGAARFSGEREYVVLTNNYGVRGLRSPDLYGQTRLM
                     FNFESMLYSPHYIYGFRPAFFVFADFGMVGESDELIYQNTLYSGFGVGLRLKNESLIF
                     PTFLFRIGYYPKLPPDAEVAYWFISTENRRNFEQFRIKEPYILQYQ"
     gene            complement(12657..13229)
                     /locus_tag="PLW22_03730"
     CDS             complement(12657..13229)
                     /locus_tag="PLW22_03730"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOU73847.1"
                     /translation="MNLSFNFIKSVSNIYDEMIDNGFSLVYLGEFSHEITKMFTSMAE
                     SDMEKNSEERSVQRKVYHVMVETLQNMNKHSDEIQERNIGKGLFIIGKKHDTYYIITS
                     NKVARKHKDHLENALITVNSATPEELKEMYKRQIREGNLSDKGGAGLGLIDIARKTGE
                     PLNYQFLQLDDQNFFFILKVEINAKKFAKE"
     gene            complement(13309..14937)
                     /locus_tag="PLW22_03735"
     CDS             complement(13309..14937)
                     /locus_tag="PLW22_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOU73848.1"
                     /translation="MKGKTLLLAAVATLTVIGCNTKKTQETEMQKKVNEFAPVELRVD
                     IISLSDKEKQMLPLLFEIAEIMDDLFWLQTYGDKEELLSKISDEATRDFVMINYGPWE
                     RLNDNKPFVEGYGEKPLGANFYPKDMTREEFEAFQAPDKTSLYTIIIRNTDGKLESVP
                     YHVAYKEKIDKAADLLRKAAELAEDEGLKKYLTLRADALQTDDYFASDLAWMDMKTNN
                     IDFVVGPIENYEDALFNYKAAYEAYILIKDLDWTQKINRFAALLPKLQESLPVEPEYK
                     KEKPGIDSDLGVYEVAYYAGDCNSGSKTIAINLPNDPRVHLEKGSRKLQLKNAMKAKF
                     EKILLPIGNMLIAEEQRDNIKFDAFFENVMFHEVAHGLGIKNLVNGKGTVREALKEMH
                     SALEEGKADILGLYMVSQLAQMGELPEKDMMDNYVTFVAGIFRSVRFGAASSHGKANM
                     VCFNYLKERGAFERIPETGTYRVNYEKMTEAMNSLAKDILTLQGNGDYAAVKKLFEEK
                     GFITDELQADLNRVAEANIPRDINCIQGKELLGL"
     gene            complement(14959..15660)
                     /locus_tag="PLW22_03740"
     CDS             complement(14959..15660)
                     /locus_tag="PLW22_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73849.1"
                     /translation="MENQNTQQKIQNLLTEKAVVKQKIYDNTLEVFGLIKEVLKEIEE
                     DFNDKLRGYDKRVKLEYNDRGKYEAELKVAGDLLIFSMHTNVFEFDRDHSIWKLSYVT
                     DNVLNSYCGIINIYNFLADSFKYNRLEDLGYLIGRIFVNREYHYFVEGKRQMGFLYNN
                     FGSAVIDKKSLRTIIETAIMYALEFDLLVPPYDVVKIASVVQLNTKIENAKLQTGKRL
                     GFRFNSDDVLEEKEQ"
     gene            complement(15771..17246)
                     /gene="proS"
                     /locus_tag="PLW22_03745"
     CDS             complement(15771..17246)
                     /gene="proS"
                     /locus_tag="PLW22_03745"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOU73850.1"
                     /translation="MAKEITSRSENYSQWYNDIVVKAGLADYSAVRGCMVIKPYGYAI
                     WEKMRDQLDRMFKETGHQNAYFPLFIPKSFFSREAAHVEGFAKECAVVTHYRLKNDPN
                     GKGVVVDDEAKLEEELVVRPTSETIIWNTYKTWIQSYRDLPILINQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATMQEAVEEAERMINVYATFAREWMAMPVVVGKKSESERFA
                     GAVDTYAIEALMQDGKALQAGTSHFLGQNFAKAFDVKFLNKEGQQEYVWATSWGVSTR
                     LMGALVMTHSDDNGLVLPPKLAPTQVVFVPIYKSDEELSAISEVVHKLRSQMHSKGIT
                     TYYDDRDNYKPGFKFAEHEMRGVPVRVAIGPKDIEKGTAEVARRDTLQKQYVPQEQLA
                     DTIVALLDDIQQNLYNRALAFRENNTVKVDSYDEFKKIVAEKGGFVLAHWDGTPETEA
                     QIKEETKATIRCIPLDMPEEEGKCILTGKPSKRRAIFAQAY"
     gene            17353..18753
                     /locus_tag="PLW22_03750"
     CDS             17353..18753
                     /locus_tag="PLW22_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73851.1"
                     /translation="MKTKLFNPALTLAVFMLASCSTGLQVSKTSSWDDEIYGTTNSKS
                     TKVQVANVSPVAPSAKSEQYNKLDGKFAEALKALEDSSKNDTVIYKAEDPNPYRRILS
                     DSYQESYERRLRGFEDPRYGIEDWSVYYSDDWRYAQAYDPSYYRVVVMGSNVWVEPWY
                     IYNSFGWPRSHFYFGFGFGWGYNPWNSYYLGYYWDPWYYNPWYYNPWYYSPYVASNAY
                     WSGWYDATYYWDKYTPNSNRYYYGRRGNTTNNTDQYVGGRSTSVIPGDGQIVTSRRTR
                     TDVPAETNPVVTTRETQTRVTPTYETRRGEGRTTSTGNQTVVRRDGTSTRVSDGTGDF
                     SSSRTSRPTREIGISEPTRRGTTPTYERPRTGNSGEYNSTRRVENPSTRTTITRNPST
                     TTPTRTTTPTYNRPNRVSTPSSSQPSTRSYDYERPRSSSSGSSSSNSSVSRSSSSSSS
                     SSSSSSSSGTSTTRRR"
     gene            18833..20368
                     /locus_tag="PLW22_03755"
     CDS             18833..20368
                     /locus_tag="PLW22_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73852.1"
                     /translation="MKTYRSLTIGFLATLSTLGYNLNAQNLADGLRFSQTHNNGTARF
                     VSMGGAFGALGADFSSIAVNPAGVGVYKSGEFTITPSFKTQKVSSTFLGVSASDNKNR
                     INLDNLGFVMSFKPYKSEDKGILAYNLSFGYNRTNDYHSNAITLGDYNNYSIMDYFSA
                     ITNGKYTPSQLEPTTNYNPFNTLGGGAWESIMARNTWLLYKYDSRTGTYEPAIYLQDS
                     VKYKNISTTEGGSGEFTTAFGVNISNKLYLGASLGITDFNYTFNSTYSEDAKESNPSR
                     PNGDRFYYMDYKQYVETKGSGFNLKIGGIYTPIPEVRIGLSFHTPTFYSFDDAFSSSI
                     ITNFDTTGTEVIFQSNSPLGRYDYHFETPMKITGSVAIIIMQKGLVSIDIEHLDYSTM
                     RFRRGGDGDTFWDLNSQMGNTFKSVTNLRFGGEYKIGDIALRGGYAYYPSPYKSGFLN
                     DKSKIQQISGGIGYRTGNVSIDFAYLRTLQDVKYVFYDLNIPSVTNKVRDSRVLLTFG
                     FRF"
     gene            complement(20435..22129)
                     /gene="ftcD"
                     /locus_tag="PLW22_03760"
     CDS             complement(20435..22129)
                     /gene="ftcD"
                     /locus_tag="PLW22_03760"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOU73853.1"
                     /translation="MSLKKLIECVPNFSEGRDMTIIKQITDEIEAVEGVKLIDVDPGK
                     ATNRTVVTFVGTPDEVVEAAFRATRKAMELIDMSKHHGAHPRFGATDVCPLVPVANIT
                     MDEVVEYARKLAKRIGEELGIPVYCYEYAAFEEKRRNLANCRAGEYEGLPKKLQDPAW
                     KPDFGPTQFLPKTGAIAVGARNFLVAYNVNLNTTSTRRANAVAFDIREKGRPMRVGNP
                     VTGKIATDENGNEIWVPGSLKACKAIGWFIDEYGIAQVSINLTDITVTPIHVAFEEAS
                     KKAAERGLRVTGSEIVGVVPLSAMLEAGKYFLRKQQRSLGIPDREIIKIAVKSMGLDE
                     LYPFDPDKKIIEYILEEKTGKKLVDRTLEGFTQETASESPAPGGGSISAAMGAFGAAL
                     ATMVANLSSHKPGWDDRWEEFSNWAEKGKFYMEQLIKMVDEDTAAFNRIMEAFGLPKG
                     TDEEKAARTKAIQDATLYAIEVPFRVMNLCFESMEVIKAMAEHGNPNSVSDAGVGALA
                     ARSAVMGAFLNVKINASGLKDKDKANEFISRGAEIVKKAQQMEAEILSIVESKIMA"
     gene            complement(22275..22994)
                     /locus_tag="PLW22_03765"
     CDS             complement(22275..22994)
                     /locus_tag="PLW22_03765"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HOU73854.1"
                     /translation="MKKNLLLPIAILISACAEISIPTNFPIPQGSGSKPLTNAEVVQG
                     LKEALAVGAKNSTGLASQVDGFYKNPKIFIPFPPEAEKVRKTLVDAGFSKQVDEFTLT
                     LNRAAEEAAKKALPIFTDAITKMSFTDAMGILRGPNNAATEYFRKNTSEQLFAAFKPQ
                     VHEAIQKVKLTSYWEPLATTYNRLTLLTGGKAVNPNLDDYVTQKAIDGLFVLIEQEEL
                     KIRKDPAARVTDILKRVFGNN"
     gene            complement(23002..24258)
                     /gene="hutI"
                     /locus_tag="PLW22_03770"
     CDS             complement(23002..24258)
                     /gene="hutI"
                     /locus_tag="PLW22_03770"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOU73855.1"
                     /translation="MQILILNIKKLVQVEDKPAVWKAGNDMAKLKTIDNAYLLIDKGL
                     IADFGPMSKAPDISSLSGDVLIYNAFGRMVFPSFCDSHTHLVYAGSREIEYIDKIKGL
                     SYEEIAKRGGGILNSAKRLAETSENELYNQSLARIYEIIRKGTGAVEIKSGYGLSTES
                     ELKMLRVIKRLKETTPITIKSTFLGAHAVPAEYKGRQSEYVDLIINEMIPLVASEGLA
                     DYIDVFCDKGFFTVEDTERILMAGIKYGLRPKLHANELDFSGGIQVGVKYNALSVDHL
                     EFTGDDEINALLGTETMPTLLPGASFFLGMVDPPVRRMINAGLPIALASDFNPGSSPS
                     GDMKFIMSLGCIKLRMLPEEVINATTINGAYAMGISDTHGSICKGKVANVFITKPIPS
                     YEYMPYAYTSDLIETVILNGSIINDK"
     gene            complement(24935..26788)
                     /gene="yidC"
                     /locus_tag="PLW22_03775"
     CDS             complement(24935..26788)
                     /gene="yidC"
                     /locus_tag="PLW22_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOU73856.1"
                     /translation="MDRNTVIGLVLIFLIMIGFGYLTQPSKEEREAYQRKADSIARVQ
                     EEEHQKRLAEEQAKALLSDSAKKQEAITQFGLFSDAASGSNKFITLENDLMRVTLTAK
                     GGRIYSVELKKYKTYSGEPLVLFTGDENTFGLQFWGDNVAVKTNDLFFTPQTTDSIIT
                     ATTDSVSVTMRLAAGGNSYIDYIYTIKPGSYMLGFDIKFTGLEKNIGNRLGYIDLIWD
                     SKLLRLEKGLENERNYTTIAYQFFTGDYEEFSSRSKEDSKELNNKIKWVDFKHQFFSS
                     IIVANDHFVNADLKAVNIDDGKHVKQFSARLALPFQNKESESIGLKFFFGPNHYKTLK
                     SYNSGFEKVVPLGRNIIRWINKYIVINVFNFLSRYISSYGLIILLLTIFIKIIISPLT
                     YKSYLSSAKMRVLKPQVDEINAKYPKQEDALKKQQAVMALYKKVGVNPMGGCIPILIQ
                     FPILIAMFRFFPASFELRQQSFLWADDLSSYDSILHLPFSIPWYGDHVSLFTLLMAVS
                     LFITSKMNTDQMGDTNAQMPGMKFMMTWMMPIMLLLWFNNYSAGLSYYYLLSNVITIG
                     QTYLFRRFVDDKAILAKLHENAKKPVTKSKWQQRLEDMAKQQEQLRKKKGK"
     gene            complement(26866..28482)
                     /locus_tag="PLW22_03780"
     CDS             complement(26866..28482)
                     /locus_tag="PLW22_03780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOU73857.1"
                     /translation="MPQSSSTKYIFVTGGVASSLGKGIISASLGKLLQSRGYTVTIQK
                     LDPYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFISLPTSQLNNVTTGKIYQT
                     VISKERRGDYLGKTVQVIPHITDEIKRRIKLLGTKNKLDVVITEIGGTVGDIESLPYI
                     EAVRQLKWELGPNNSAFIHLTLVPFLSASGELKTKPTQHSVKELLENGIQPDVLVLRT
                     EKHLSDDIRRKVALFCNVEPEAVVESIDVKTIYEVPLLMLKEKLDLTILRKLNLPASE
                     PKLDDWINFVNRVKNPSHRVKIGLVGKYVQLPDAYKSIIESFIHAGAANDCKVELELI
                     QSEDINASNLSEKLGHLKGILVAPGFGHRGIEGKILAVKYARENKVPFLGICLGMQCA
                     VIEFARNVLGLTDANSTEMVRTTPYPVIDLMEDQKGITDMGGTMRLGAYPCVVAKGSN
                     THKAYKKLEISERHRHRYEFNNKYFEQFQNAGMIPVGINPDTNLVEIMELSDHPWFVG
                     VQFHPEYSSTVLKPHPLFVDFVKAAINFNK"
     gene            complement(28494..30491)
                     /locus_tag="PLW22_03785"
     CDS             complement(28494..30491)
                     /locus_tag="PLW22_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOU73858.1"
                     /translation="MNTNELNDPKDQNLENIEGTIVSSNAGNHDVADSVNDLVDVNAD
                     KPMSISDEAYDEEPNDEELKPLEMDDEHVDFDEEDEEEAEIDEEDDEPHVDYSNLSKD
                     ELVQTLKDLINNRPVQKIRQKVESLKSIFYRKHKAEVEKLRKEFVDAGGDLEQFSLPE
                     DNAEMQFKELLKQYRAKRAQYNNQLEEQKQHNLEQKQQIIEKIKDLVNSTEPVGKIYQ
                     EFRDLQKQFLEVGPVPQASLNDLWNNYHHHVQAFYDFVKISKELRDLDFKKNLEAKIK
                     LCEQAEELLLEPSVVVAFKKLQKLHEQWREVGPVPRENQNEIWERFKEATTKINKKHQ
                     EHFESIKEQQKKNLEAKAELCEKAEELAAQNPKSVKEWNKLSKELIELQKLWKTIGFA
                     TKKENNKVFERFRSACDTFFNKKRDFFKDIRDVQVNNLQLKTELCLQAEALKESTDWK
                     KTTEDLINLQKRWKQIGPVPRKNSDEIWKRFRAACDYFFEQKSKHFASVDNSYEENLK
                     AKEALIAEVEAFEFGEDMEQNLNALKEFQRRWTEIGFVPIKVKDDVQKRFRAAIKKHF
                     DKLNAQEGKNKVVRFQAKLEEAKANPRAMSKLRQDRERMFNRMRQLENDLTVWENNIG
                     FFAKTKNAEAMIEEVKRKIERAKEEIAELSEKIKLIDKLEQ"
     gene            complement(30673..31113)
                     /locus_tag="PLW22_03790"
     CDS             complement(30673..31113)
                     /locus_tag="PLW22_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU73859.1"
                     /translation="MTLVSFYNPLASRRTGVDFERVFESFVKSASEMANARLSYPAVN
                     IYEEPERFRLEVAAPGYQKDDFKVSIDNDMLKISAEKENNPVEGEAYTRCEFLTGSFE
                     RTFVVGKAIDTNKIDARYENGILTVFLSKRDEAKPQKPRSIKID"
BASE COUNT         9292 a         6949 c         6246 g         9222 t
ORIGIN      
        1 tgagtcacgc cacaggcgtg gcgaattgta tcgcgtggca gttccacccg cggggcaccc
       61 atcgggtcag gcttttcagc cttgattttc tttggttctt tcttgtatca agacaagaaa
      121 gaactttaaa atagctcttt gcaatgtttt tcctttgcga tgcaaagctt cacccttgct
      181 ctttggaaaa tgtattgcgt gcatcgcaat acaagtggga gtatacatag gtttacaaac
      241 gtttagcccc tacggggcta gaagcggtgt tcgttgttcg tgaatcgttg ttcgtgaagc
      301 tattgtcatg ttgagctatg tgaaacgtct catggggacg attaagagat ccttcgctgc
      361 gctcaggatg acaggcatgt ttatttttgt taactaaacg acattgatta ctggaatgat
      421 gaaataaata atttactgtt agtaactcaa ggtataaaag aaatatttgc tggacacttt
      481 ttccattgga taaccaaaac aggccaacgc attttaaaaa ttatcgagtt agtttttcct
      541 tatttcaaac agatactcca aagcctcagt aaactcccgg ctccaaaacc attctgaatg
      601 acgaccatca ggtgcaattt tcattttaac ataatcctca tccttatatc cagctttgta
      661 taggtggtgt tccagcattt tcatatcctc aactaaggtt tcgctttcct tttcaccagc
      721 caaaagatat attttctgat cagccctacg cttgtacttt gaaacatact caaatacttt
      781 aggcgaaaac cagagcgaag gtgaaaacac tcccacccta ccaaaagcat cgggatactt
      841 gagccctata taaagcgaaa ttaatccacc cattgagcta ccaataacag cattaaactt
      901 gggttcagga ttagtcctat agtttttatc gataaatggc ttaagctctt tggcaacaaa
      961 gtatgcatac ttatcgccat cgccacccat gccaagggag tcgttacgcc atggtgagta
     1021 ttcgtttata cgctccttac tatcattata aatagctaca acaatggcgg caaagccgca
     1081 gctgtagtaa aggctatcca ttatttcgtc aaccctccat tccccggcaa atgaggtagc
     1141 ctcgtcaaaa acattttggc catcgtgcat gtaaattaca gggaactgct gtcttttgct
     1201 gtagtttggc ggtaagtata accgcaccgc tctacggcga ttcagctgtt tcatctcaag
     1261 ggttgagggt aagaacctaa cattagttga agctgtggat tttcgttgag cggcctcaaa
     1321 catatcgcgc catccattca ccttaatact aacggtatcg gtaggtggga aataaatcct
     1381 gtttcgaata tcgcccccat tggagtccac ctcaacaagt tgccaactgc cacgtgaaag
     1441 tttatactca aaggtatcct ttacatgcgt taatttcagt tcataacggt tcgtagttcg
     1501 tttaaaggcc caactaatat catgcggatt ccatccattt agttccgatg ccataaaaat
     1561 tgtgtcatcg ggccgagttc ttgccggaaa agaatcaaca acaaacacaa cctgtgcgct
     1621 taaccggaaa ctcaataaaa tcaacgatga cagtataaca aaattggctt tcattcttag
     1681 agttgtttac attaaaaaca aaggtaacac taaaaaagta gttgtaaaaa tggctaaaca
     1741 gctatatctt tgcaggaaat aaatggcccc gtagttcagc tgaatagaac gtcagattcc
     1801 ggttctgaaa gtcgggggtt tgaatccctc cggggtcact taaaaaaagc cagccaaaga
     1861 ggactggctt tctttcttga ataacaatac actaacttat aggcccggag ggatcatgga
     1921 gcgtagcgga actaatccct ccggggtcac ttggttaagc cagtcgaaca ggactggttt
     1981 tctttgttca aaaaagcaac cataatcagc attgccggag ggatcatgga gcgcagcgga
     2041 actaacttga ggttcatcac ttttgggaat tcattctaac tcacttcaca ataatttact
     2101 tatattggcg ttaaccaaaa aaatcgcttc acaatggaaa actcaattag gaacaacctc
     2161 gacaatcctg aagtgcttga gcaactttac agaaagaaca gaaaaggttt tgaaaaagcc
     2221 tttttcagca tttactctga aatatcaagc attccattag caaaattttg gtttaccagg
     2281 ctaaccgccg ataataaaat actgttcaat gtaaacctaa aagaaattgc atatgtatta
     2341 atttcctgtt tgatagtcag tattgtcatg ttaattcctt ggttgtttgg gtttaaccca
     2401 catgaaaata atttttacga acggaacctt gcgctaacgg ctctcttggg agtcaccctt
     2461 tacacggcca tactaaaaaa tatcaaccta aaaaaaactg cagcaatcgt tggatttttt
     2521 attatttctg caatttatgt aaatgttctg ccaaaaacgg aaagtagctc taccaccctg
     2581 atatacatcc atttacccat acttctttgg tttatttttg gcatagtatt ctcagagttc
     2641 gaactaaaaa acaatgaaaa acgaattgag tttataaagt ataatggcga cttagctgtt
     2701 ttgtttagca tttttgcaat agcaggaatg atcctaactg cttttaccat tgggttgttt
     2761 agcgcaattg gaataaatgt tgaaaaaatt tattttgaac taattgttat acctggagta
     2821 gtttccgttc caataatttg cacatatctg attaataaat tcgaatacct atcaaataaa
     2881 attgtcacta tagttgccaa tatttttagc ccactaatac taataacact cataatatac
     2941 cttatcagca tagtagcaaa aggaagcaat ccttttgtta atagggattt tctaattatt
     3001 ttcaatgcaa tgcttattgg agttgttgca ttggtgatct tttcaatttc agaaagagtt
     3061 aacaactcaa caaataatta ttacacaatc attttcactg ctctttcaac aatagctttg
     3121 ctaataaatg ttgtagcgtt actggctatt ctttatagga ttacggagta tggcataaca
     3181 cctaaccgcc tagctgtaat aggatcgaac cttctatttt tcacccattt aattctaatc
     3241 ttaaccaatt tattgagagt gatacgaaaa aaacaacact taaccattat tgagcaagta
     3301 acggctaaat ttttacccta ctatggaata tggttgattt ttgtggtttt tattctgccc
     3361 tttatattca atttcaagta atgtaatttc aataaatttt gaacacaaca ctatttaaca
     3421 tggcaaataa ttaaaaaagc aataatttag tgcgaacttt taaaaacata gccatgaaaa
     3481 gagtagtaat tagttcgtta ttattatttg ccacaacagg actgtttgct caaatttttg
     3541 tgggtggtag cgttggattt aacaccactg gcggaaaaat tgaaaatggc aacacttccg
     3601 ttgacaaggt tacacaaacc agcttctcac tagcccccaa agcaggaatg ttcctatccg
     3661 aaaagcttgc agttggagcc atactaggtt ttaacctaca aagtgagaaa acgcctggca
     3721 cccctgaaga aattgataga actacaacat ttgggttaac gccgtttgcc cgttactacg
     3781 ccttttccct aaacaaattt tcggtttttg ctcaagggaa tttaggtttt tcgtatggtg
     3841 ttgaaaagca caaggagggt tcaacaacta caactggccc taaaacaacc accattggga
     3901 tatcggcatt tccaggcatt tcatataaat tgactgataa agttgagctt gaagcagtaa
     3961 ttggtggact taaccttaac tttaaccgtg tctcggttaa aaataacaac acaaccaaca
     4021 tcaccaatac ttttggagtt ggcgctaacc tcgatgccat tgcaaccaca gggtttatta
     4081 caataggtgc aattgtaaag ctttaatacc tacccgataa gttaaaaagg gatgcaaatg
     4141 cgtccctttt tatttttctg cttttttact gattttcttg tattccttac aaaccagtgt
     4201 taaaccacat tctgtgcact tgggtttccg tgcagtacac acatacctac cgtgaagtat
     4261 aagccagtga tgagctttag gaataagttc aagggggaag ttttccatca gctggcgttc
     4321 aacggcaagc ggggtcttgg cgttgcttac caaaccaatc ctatttgcta cacggaacac
     4381 atgagtgtca acagccatgg ctggtttatc gaacactacc gatgcaataa cattggctgt
     4441 tttacggcca acacccggaa ggcgttgcaa tagattgata tcagaaggaa cctcgccatt
     4501 aaactcagtc atcaaaacct tggccattcc tagcagattt tttgccttac tgtttgggta
     4561 tgagcagctt tttattattt tgtaaatatc ttccacgctg gcgtttgcta aactcacagc
     4621 atcgggaaaa cttttgaaaa aatttggtgt tattagattt acacgtttat cggtgcattg
     4681 cgcactaaga ataactgcca caagcaattc atatgggtta gagtaatgca attctgtttc
     4741 ggctatggga cgggttttct caaaatactc tattacaaaa cggtatagtt ccttttttga
     4801 attcatcata tttctcttta actccaaaat tagattaaat tgattaaacc ttttttattt
     4861 ccactggcaa atcacctcat aaaatattta agaatcagca tttttatcag cccgtatgga
     4921 tttgcaaatt cattataatt cagtttaaca gatcactttc cactattcct ctaacttcat
     4981 catattctgt ccattccgac tcattccgat tcattccgac tcattcatct aaaaatccta
     5041 ctatctccat ttttatcagc ccgtatggat ttgttaattc atggcaattc ggtttaagca
     5101 aatgttgcac aaattactaa ctttgcaact caaaaaaaga atactttggc attaatttac
     5161 ctacaagctg aaaatgtaag caagtcgttt ggtgatttgg aagtattaag cgatgtatcg
     5221 cttagcctat tccaaaacca gcgcacagcc ctagtagcaa gaaatggaac aggtaaaagc
     5281 acattgctta atatccttgc aggatatgac acacccgatt ccggaaaagt aaccctacgt
     5341 aacgatatta gcgttggcta tctaccacag gaacctcaac tcaatttatc aaaatcggtt
     5401 ttagaaaatg tttttaatgc cgatgatgct atttcggttg ccatacgtaa ttacgaaaaa
     5461 gctattgagt caaccgataa aatggcattg cagaatgcca ttgatgagat ggacaggctt
     5521 aacatttggg atagggaaca aaaggccaag caaatactct cagtttttca gcttacagat
     5581 ataaatcaac ccgttcaact gctttcgggt ggtcagcgca agcgagttgc acttgctgcg
     5641 gtgctgataa gcaaccccga cctgcttatc ctcgatgagc caaccaacca cctcgacctt
     5701 gaaatggtgg aatggttgga ggattaccta acacggtcgc aaaaaacagt gctgatggtt
     5761 acccacgaca ggtatttcct tgaccgcgtt tgcaccgata ttattgagat agatcaaaag
     5821 caggtttata cgtacaacgg aaactattcc tattttattg agaaacgcga tgagcgaatt
     5881 agggcaaagc tggcagaaat tgacagagca agcaacttac tccgacggga aactgaatgg
     5941 atgcgccgca tgccgcaagc acgaggaaca aaagccaagt atagaattga tgcattctac
     6001 aagctccgcg aaactgcact acaaaaaatt cattccaatg atgttgaaat aggcgtaagc
     6061 gcctcgcgcc taggaaaaaa aataatggag gtccataatc tttcaaaaag ctttgacagt
     6121 aaagtaattc taaaagactt cagctacacc ttcaaccgct ttgaacggct tggtatagtt
     6181 ggtgctaatg gcactggtaa aactaccttc cttaacctga ttacaggaac actccctccc
     6241 gattccggaa caattgaccc cggtgcaact ctgaaaattg catattaccg acagagcgga
     6301 atgaacattc ccgaggatgt taaggttatt gacgttgttc gcgaaatagc tgaggtaatc
     6361 acccttgcaa atggtaaaac tataaccgca tcgcagtttc taaataactt tctatttaca
     6421 cccgacaggc aacacactct ggttcgtaag ttaagcggtg gcgaaaagcg caggctatac
     6481 ctgctaacag tgcttatgca aaaccctaac tttttaatac tcgacgagcc gaccaacgat
     6541 ctcgatattt ttacgctcaa tgttttagag gattatctga ttaactttcc tggcgtagta
     6601 gttgtggttt cgcacgaccg gtattttatg gataagctag ttgatggtct gttgattttt
     6661 gagggcaatg gggaaattag cggatttccg ggcaactata ctgaataccg tgaatgggca
     6721 aaggaacagg aaaaaagtaa tgccgaacaa caaacctcct caaaagggaa aactgatagc
     6781 aaacgccccc aacctgaaag gaagaagagc aagttaacct ttgcccaaaa gaaagagttg
     6841 gaaaaactta ccgatgaaat agtaaagctg gaaaaggaaa agaaagaaat tgaaggtttg
     6901 ctcaacggtg gcattcagga tgctaagctg ctaatggaca tttcaaatag gtttagtgaa
     6961 atctccaaac tactcgaaga taaagaaatg cgttggttgg aattgaatga atttgaggaa
     7021 taaccatgca atcactcatc aaatttgata agtaaaatct tttgggtatt ctcatcaacc
     7081 ctaaacctat tatcaattcg gtaaccaaca acccatacaa tttggttttc cgaagtcaga
     7141 acaaaaacct tttccttatc tggaaggggt attttgatgt caactaggaa atcgctaatc
     7201 tttttaaagc tggtcatgcc gaaaggcata aaccggtcac caggttgcca tggtctgatg
     7261 gtaagtggaa acttgagttt ttccagatca agatatgcgt attttgtatc tttcccaaac
     7321 acaaaacctt gagagtaatc aagtttactc aaacttaatt ttacaggttg gtcaatttga
     7381 gttatgttga catcaatctg acattcaacg ctttgcctct tttgtttttg ctgtaaaata
     7441 aggtgattcc tatcgcggaa aattacatga gtatcggact ctaccctttt gccaattgac
     7501 gattctaata gttcgcaaac ctcagtagtc agggcagggg tgaaattaaa gtccgacaga
     7561 aaatcaacta aaaaaatatt gacagcggcc tggttcttca gtttttcaat atcgatatag
     7621 aatacccccc cattttcatt aacaatttcc ttacgtaatt gagtcaaaaa gctatcaaca
     7681 ataagctgag cttcacgaag atgctgtgcg gtttgataaa ttgagatatc gacagagggg
     7741 tttaactcac gcaaaagagg caaaacattg tgcctaatat agttacgctt gtacttatcg
     7801 gatgcgttac tggaatcctc gcgatacgaa atgttatgta aggtagcata ctcttgaacc
     7861 tgaacacggg tagcaaaaag cagtgggcga atgatattcc cgtttacggg tttgattccg
     7921 gtaagccctc ttaaaccagt tccccgacta aggtttagca gcactgtttc aacggaatca
     7981 tttagattgt gagctatggc tattcgggtg tatttatgtt ccttacaaat ctcatcaaag
     8041 tatgcataac gcaattcccg agcagccacc tgaatggata agccattctc cttggcatat
     8101 tccaaggtat cgaacttaat ggtatgaacc ggtaagccat tcatcaggca atggtaacgg
     8161 acaaactgtt catcggcatc cgattcatcg ccccgcaagc taaagttgca gtgagcaaca
     8221 ccaacactat acccggcatg gaccaatagg tgcaatagca ccatcgagtc gataccacca
     8281 ctaaccccta ccaggatttt atcctggtca tttgccattt ggtgctcacg aacaaaatcg
     8341 atcattttac tctgcagcat ctatctgagc gtttagggtt tctaaaatgg cttttgcctt
     8401 aactaagcac tccctgtatt cagcctcggg ttgcgactta aaggttattg ctccaccaac
     8461 tgtaaacgac aaataaccat tgcttctatt ataaagcaag ctacgaatta ccacgttaaa
     8521 atcaaaattg ccgttgggca taaagtaacc aactgcaccg gagtatagcc cgcgtttgga
     8581 acgttcaaaa ttctcaatta gttccattac cctaactttt ggggctccag tcattgaacc
     8641 catcggaaat gttgctctaa aaggatcggt tgggcgcagc ccatcggcca gctcgcacga
     8701 cacggttgaa atcatctggt gcacctgtgg aaatgtgtaa atcccgcaaa gctcatttac
     8761 cctaacactc ccgcgcttgg ctattcgcga taaatcgttt ctgaccaagt cgacaatcat
     8821 tacattctca gcacgttcct taggatcggt tttaagtcga aatatctcct gctgatcaga
     8881 ctcgtccgat tccattcgcg gtgctgtacc cttaattggc tggctgaata gccaatcacc
     8941 atcgcgacgc aaatagcgct ccgggctggc acacatcaga aacttatcgt ttaccctaag
     9001 aaatgcgcca aaaggcgttg gcgacctgtc gtaaagctta ttgaaaacaa cataaggagt
     9061 aattcttaca ttgttagcaa agaattcctg gcataaattc aactcgtaaa catcaccccg
     9121 ggcaatgtgc tgcttgactg cgctaaccgt attcaagtaa tcgttatagg aataatggga
     9181 acggaaaaca accgacacat tgtggtccaa ttcctccaag tcaccttcta gcctatcaat
     9241 caaatcactt acttcttctt gtgatacttc ttgctgaagg taatgaaact cagcaatttc
     9301 accattgacc agtattagta tccggggtac aaagaaaaac atatcgggta aaccaactcc
     9361 atcggggtta ttggactgta aatgttcgag ttgattttta agatcgtaag taaaataacc
     9421 aaaaacccaa tcattatgag cgcttaggaa ctcatcgagt tcatcaaaat gattagaatt
     9481 tggactgcaa gccgagctaa tacctaatgc agcagcaagt tgaaaacggc tggtaaaatt
     9541 ggtttccgga atagtgaacc tatcggatcc ccgaagaaga atacccacat cggtgttttc
     9601 aacctcatac gccagatttt tggcaaacct atcaaaattg cttaccctat ggatgtattt
     9661 actgcgcata agcgcaaagg tagccaatta tgggttcatg aagagtgggg atggtttaat
     9721 ttttcttgaa ctggttcacc atatcaacaa ctgtatgttc aaaaggggta tatcccaaac
     9781 ccaactcctg cctgcttcta ctagtactga acgcaattcg atacccaaca ttacgggcta
     9841 caaaccgagg tgaaaggcca aaaagagggc taacaaagta aagtaaccac ttaggggcga
     9901 ccattttggg gagtttaaac tgaccaggga atttattgtt gataattgca accagttcca
     9961 tgaaatttgc cactcgttca gcaataataa atcgaccaga aacagaaggg ttttcaagag
    10021 ctagcacatg tgctttagca acatcgcgta catcaacaaa accgaagtaa aggtcaggtg
    10081 cgccaaaacg atacttaccg gaaagcaaat ccttcatgat ttgaaggctt tccgaattgg
    10141 aatttcctgc gagtgagggc ccaagaacca aagcggggtt tattaccact aactcccatt
    10201 tactctgttc cgcattaatt cgccatgcct ccctctcagc ctgaactttt gaaaaggagt
    10261 aaggttgatg cttcaatgaa cttgtagtgt tataattttc ctcagtaaac tcggttaatc
    10321 cttgttcctc catgtcgata ttatcgccat gaacagcagc cacgctcgag gttagcacta
    10381 cccttttcac tgtcgacgat ttgcttgcag cacccaaaac atttcttgta cccttaacag
    10441 cagggtcaac caaatcgcgt tgcgggtctt taaaccgaag agtaaatggt gaggccatat
    10501 gtattatggc atcggtaccc tcagctgctt catcaaaagc tccatcaacc agcaaatcag
    10561 cctcaaaaag ttcaagtaaa ttagtggaat ttggcaaatt ctgcagatgc aggtacttgt
    10621 cctttctgtt aaacgaccta acaggtaccc ttacgcgata acccttttca agcaattcct
    10681 taacaaccca tgaaccaata taaccggttc ccccggttac aagtatagtt tttgtcatac
    10741 atcaaaattt tatgctgttt aagaaacaaa acagtatgaa aaaagttttt aagtatgaat
    10801 attactgata ttgaagaatg tatggttctt taatcctaaa ctgctcaaaa ttccttcggt
    10861 tctcggttga aataaaccag tatgccactt cggcatcggg tggcaactta gggtagtaac
    10921 caatacgaaa aagaaacgta ggaaatatca aactttcatt cttcaacctt aaacccactc
    10981 caaacccact atacagggtg ttttggtaga ttaactcatc ggactcaccc accataccaa
    11041 aatcggcaaa aacaaaaaaa gcaggtctaa atccgtagat gtaatggggt gaatataaca
    11101 tggactcaaa gttaaacatc aatcgtgttt ggccataaag gtcgggactc ctaagtcccc
    11161 ttacaccata gttgtttgtt aaaacaacat actccctttc accactaaaa cgggcagccc
    11221 ctcgagtaaa atcgtaccta ataaaatggc gaatatcgct acggccaatg tggtaaaggt
    11281 tagagaagta ctgtgctcta aggttgataa cagcctgctc taccttaggc ccctcagcaa
    11341 gataaccccc tagccttgac gatagaaaca aataacccca cggtgtaacc tcggctgctg
    11401 aaagctcccc tccaatcaaa aaacgacgct gaaatgtact ttgttgaata ccgcttgtgg
    11461 cctgaatttt aaaaccaacc gggatatcct cagtgttccc aaatccaaaa tataagctgg
    11521 agcgataaat gttttggttg gataacccca gcgaaacaac ataattttca gccgaatgaa
    11581 atattggatt taggtaaata gaaaccggtg gtgattgaaa gtaatcgcgc ctatagtaac
    11641 gtaatgcaaa aaccagattt agaggggcac tggtaccaga gcgtctacta accctgactg
    11701 atcggccaat ccaccagtcc tgcgactgat atgaaatttg tactactgaa tcgtcggtga
    11761 aaaccctgta aggttcgtcg gactttaaaa gttcaacacc tcctgcatac ttagtcttgg
    11821 atgcaaagaa atcacggtta aacctcataa ggtaagtctc ataggaatga ccttttctta
    11881 caaaaaagct actggaaatg tatgaaccaa aatagtttgg tacatcaaac tccccttgat
    11941 acgaccattt atcggtaaaa gtcttattat aataagctat tgctttagct cgaataccta
    12001 aacccgcaaa gttcctttcc gataaagcaa gtttcatctt ttcaagggat gagtattgca
    12061 caccaacgga cattgaccaa atatccttgg ttatcacata aacatgcaca tggtttgaat
    12121 cgctcaagag ttgaactaaa atattggcat cctcaaagaa gttactttga cgcagcaact
    12181 gctcagtttc agccaaatcc aatggaacaa ccttatcgcc ttccttgaaa agcaagttgt
    12241 tttgaataat ttttttccag gtttttaaat gcaagctatt cccaaatttt tcaatactcc
    12301 tctctggttt aatagtagta tcgtaaaccg agtatccaaa tgcatcaaaa cgtactatgc
    12361 taatctgtga aattaccttg ccttcatact tcaaaaatcg gttaacagtt tcctcattgg
    12421 ttaatggtgc aacattaaca ttctgacggc caaaccactt atatacggtt tgtgaaattt
    12481 tatcatcttt ctttttaata ctatcagaac taacctggtt gctatcagat tgtgcaaaag
    12541 atgaataatt aataaatcca aaaaatgaaa aataaaccat cattactaat gcaatgatgg
    12601 ttttaatacg gacgtatgaa agcaacctaa aatcagtcat tcaacaagaa ataagcttac
    12661 tccttagcaa attttttggc attaatttca actttaagga taaagaagaa gttttgatcg
    12721 tcgagttgaa ggaattggta gtttaatggt tcacctgttt tacgagcaat atcaattagc
    12781 cccaagccag ctcccccctt atcggaaagg tttccttcgc gaatttgccg tttatacatc
    12841 tccttaagct cctcgggtgt tgcgctatta acggttatca gtgcgttttc aaggtgatct
    12901 ttatgctttc gggctacctt gttggaagta ataatatagt atgtatcgtg ttttttgcca
    12961 atgataaata accctttacc aatattacgc tcctgaatct catcggaatg cttattcata
    13021 ttctggagag tttcaaccat aacgtggtaa accttacgct ggaccgagcg ttcctcacta
    13081 tttttttcca tatccgattc cgccatcgat gtaaacattt ttgtgatctc atggctaaat
    13141 tcccctaggt aaaccaggga gaatccatta tcaatcattt cgtcgtaaat attcgagacg
    13201 gatttaatga aattaaatga taggttcata ctccagaaat tttatattgc tttcaaaaat
    13261 agaataaaaa aggtcggaaa gcaaaccatc cgaccttaat ttttaggttt ataaccccag
    13321 caactctttt ccctgaatgc agttaatatc acgtgggata ttagcctcag ccaccctgtt
    13381 cagatcggcc tgaagttcat cggtaatgaa tcctttctcc tcaaagagtt tcttaacggc
    13441 agcatagtcg ccattaccct gaagggttaa aatgtccttg gccaatgaat tcatggcctc
    13501 agtcatcttt tcatagttga cacgataggt tcctgtttct ggaattcgct caaaagcgcc
    13561 acgttctttc aggtaattaa agcaaaccat gttagccttt ccatgcgacg aagctgcacc
    13621 aaaccttacc gatcggaaaa tacctgcaac aaaggtaaca tagttgtcca tcatgtcctt
    13681 ttcgggcagt tcgcccattt gtgcaagctg cgataccatg taaagtccaa gaatatcggc
    13741 tttcccctct tccaaagcgg aatgcatttc ttttaatgcc tcacgaaccg tgcctttccc
    13801 gttaacaagg tttttgatac ctaacccatg ggcaacctca tggaacataa cgttctcaaa
    13861 aaaagcatca aacttgatgt tatcacgctg ctcctcggca atgagcatat ttccaatagg
    13921 cagaagaatt ttttcaaact tagctttcat agcattctta agctgcagct tgcggctacc
    13981 cttttccagg tgaacgcgag gatcgttggg taaattgata gcaatggttt tgcttccaga
    14041 attgcaatcg cctgcatagt atgcaacctc ataaacacct aaatcagaat cgataccggg
    14101 cttttctttt ttgtattccg gttcaactgg taagctttcc tgaagttttg ggagtagtgc
    14161 agcaaaacgg ttaatctttt gagtccagtc taaatccttt ataagtatgt atgcttcata
    14221 agcagcctta tagttgaaca gagcatcctc gtagttctca attggtccaa ccacaaagtc
    14281 gatattattg gtcttcatat ccatccatgc caggtcgctg gcaaagtagt catcggtttg
    14341 caaggcatcg gcacgaaggg tgagatattt tttgagtcct tcatcttcgg caagttcagc
    14401 ggctttacga agcaaatcgg cggctttgtc aattttttcc ttgtaagcaa catggtatgg
    14461 taccgactca agcttgccat cggtattacg aataataatt gtatataggc ttgttttatc
    14521 gggagcctga aaggcttcaa actcctcgcg agtcatatcc ttcggataaa aatttgcgcc
    14581 aaggggcttt tcgccataac cctcaacaaa aggtttgtta tcgttaagcc tttcccatgg
    14641 gccataattt atcattacaa agtcgcgagt tgcttcatcg cttatcttgc taagcagttc
    14701 ttccttatcg ccataggttt gtagccaaaa taggtcatcc ataatttcag caatctcaaa
    14761 aagtaaagga agcatttgct tttccttatc ggaaaggctg attatatcaa ccctaagctc
    14821 aaccggagca aattcgttaa cctttttctg catttcggtc tcctgtgttt tttttgtatt
    14881 gcaaccaatt actgtgagcg ttgcaacagc tgcaagtagt aatgttttcc ctttcataat
    14941 aaaatttata ttgatagatt attgttcctt ttcctccaaa acatcatcgg agttaaacct
    15001 gaaacccaaa cgtttaccgg tttgcagctt agcattctca attttagtat ttagctgcac
    15061 taccgaagct attttaacca catcgtatgg tggaaccagc aagtcgaatt ccaatgcata
    15121 cataatggcc gtttcaatta ttgttcttag cgatttttta tcgataaccg ctgaaccgaa
    15181 attgttgtaa aggaacccca tttggcgttt cccttcaaca aagtagtgat actcacggtt
    15241 tacaaatatt cggccaatca gataaccaag gtcttcgagc ctattatatt taaaggaatc
    15301 ggccaaaaag ttatatatgt tgataattcc gcaatatgaa ttgagaacat tatcggtgac
    15361 ataggatagt ttccatatac tatgatcgcg gtcaaactca aatacgttgg tatgcatgct
    15421 aaaaattaac aaatcgccag ccacctttaa ttcggcttca tattttcccc ggtcattgta
    15481 ctccagctta acccttttat cgtaccccct aagcttatcg ttaaaatctt cctcaatttc
    15541 tttgagaact tccttaatca atccaaaaac ctcaagggta ttatcataaa ttttctgttt
    15601 tacaacggct ttctcggtaa gtagattttg aattttttgc tgtgtgtttt ggttttccat
    15661 aaatattcag tttaagaatt tcaaatatac attaaatatt aaaagtaaac aaagcaaaaa
    15721 aggctgcgtt ttgcagccca ttttattaaa attaaacatc tcataggtta ctagtatgct
    15781 tgagcaaaaa tagccctacg tttcgatggt tttcctgtta aaatgcattt tccttcctcc
    15841 tctggcatat cgagcggaat acagcgaata gttgcttttg tttcctcttt aatctgagcc
    15901 tcggtttcgg gagttccatc ccaatgagcc agaacaaagc ctcccttttc tgctactatc
    15961 tttttgaatt cgtcgtagga atctacctta acggtattgt tctcacggaa agccaaggcc
    16021 cggttgtaga ggttttgctg aatatcatca agtaaagcaa ctatagtatc ggcaagttgc
    16081 tcctgaggca catattgttt ttgaagggta tcgcgacgag ccacctcggc tgttcccttt
    16141 tcgatatcct ttgggccaat tgcaaccctt actggaacac ctcgcatttc atgctctgca
    16201 aatttaaagc cgggcttgta gttatccctg tcgtcgtagt aagtggtaat ccccttggag
    16261 tgcatttgag atcttagctt gtgaacaacc tcactaatgg ctgataactc ctcatcgctc
    16321 ttatagattg gcacaaaaac cacctgagtt ggagcaagtt ttggaggcag caccaaacca
    16381 ttatcgtcag aatgggtcat aaccagggca cccataaggc gggtagaaac accccacgat
    16441 gtagcccaaa catactcctg ttgcccctcc ttgttcagga actttacatc gaaagccttg
    16501 gcaaagtttt ggccaaggaa atgcgaagtt ccagcctgca aagccttgcc atcctgcata
    16561 agcgcttcaa tggcatatgt atcaacggcc ccggcaaaac gctccgactc cgatttcttc
    16621 ccaaccacaa ccggcattgc catccactcg cgggcgaatg tggcatacac atttatcata
    16681 cgttcggctt cctccacggc ttcctgcatg gtggcatggg cggtatgacc ctcctgccac
    16741 aaaaattcag cagtacgaag aaacaggcgt gtacgcatct cccagcgcac tacgttagcc
    16801 cactggttaa ttagtatagg caaatcgcga tacgactgta tccatgtttt gtatgtgttc
    16861 caaattatgg tttcggaagt gggtcgaaca accagttcct cctcaagctt agcttcatca
    16921 tcaaccacta cgcctttgcc gttagggtcg tttttcaagc ggtaatgtgt aaccacagca
    16981 cactccttag caaaaccctc aacgtgagct gcctcgcggc tgaaaaacga ttttgggatg
    17041 aatagaggaa aatatgcatt ctgatggcct gtttccttaa acattctatc gagttggtca
    17101 cgcatttttt cccaaatggc ataaccgtat ggtttgataa ccatacatcc ccgtactgca
    17161 gaataatcgg ccagtcctgc ttttaccaca atgtcgttgt accactgcga ataattttcg
    17221 cttctgcttg taatttcctt tgccatatta aataattgga atgatttttg ctgtaatcaa
    17281 tatgaaaaca ctggcaaaag taattaataa aaatgaatta ttaccctaac cactaactaa
    17341 atggaggtgc atatgaaaac caaattattc aatccagcgc ttacccttgc agttttcatg
    17401 ctggcatcct gttctacagg gctgcaggtt agcaaaacat catcgtggga tgatgaaata
    17461 tatggaacaa ctaattcaaa atctacaaaa gtacaagttg caaacgttag cccagttgca
    17521 ccttcagcta aatcggaaca gtataataaa ctggacggca aatttgctga agccttaaag
    17581 gcattggaag atagcagcaa aaacgatacc gttatttaca aggctgaaga tccaaaccct
    17641 taccgtagaa tactttccga tagctaccag gaatcgtacg agcgtaggtt aaggggcttt
    17701 gaggatccac gctatggtat tgaagactgg agcgtttact attccgacga ctggcgttac
    17761 gcccaggctt acgatccttc atactacaga gttgtagtaa tgggaagtaa tgtatgggtt
    17821 gagccctggt acatttataa ctcttttggt tggccaaggt cgcatttcta ctttggattc
    17881 gggtttggat ggggctataa cccatggaac tcatattact tagggtatta ctgggatcca
    17941 tggtactata acccttggta ctacaatcca tggtactact ccccatacgt tgcaagtaac
    18001 gcatactggt caggatggta cgatgcaacc tattactggg acaagtacac gcccaatagc
    18061 aacaggtact actatggccg aagaggaaat acgaccaaca ataccgatca atatgtaggc
    18121 ggaagaagta catcggtcat tccaggtgat ggacaaatag ttacatcccg taggacaaga
    18181 acagatgttc ctgctgaaac aaacccagtt gttacaaccc gtgaaactca aacacgagta
    18241 acgccaacct atgaaacccg caggggcgag ggccgaacta cctcaacagg taatcaaact
    18301 gttgtacgaa gagatggaac atcaactcga gtatctgacg gcacgggcga tttttcatct
    18361 tcaagaacat cacggcccac ccgagagatt ggcatatcgg aacctacccg caggggcaca
    18421 actccaacct acgagcgccc acgaactggt aattctggcg agtacaacag tacccgtaga
    18481 gttgagaacc catcaacaag gacaactatt actcgtaacc catcaactac aacacctacc
    18541 cgaactacta ctccaaccta caaccgccca aacagggtta gcaccccatc gtcatcacaa
    18601 ccttcaaccc gtagctatga ttatgaacgc ccccgtagtt caagtagtgg ttcctctagc
    18661 agcaattctt cggtatcccg ttcatcatca agcagttcat caagctcatc aagtagcagt
    18721 tcaagtggaa cttcaactac tagaaggaga tagttaaata tgcacctcaa gggagaggtt
    18781 ctcctctccc ccttttttgt cttaaaaaga ttaattacat aaaaaaacaa acatgaaaac
    18841 atacaggtct ttaacaattg gctttttagc aaccctatca actctgggtt acaaccttaa
    18901 tgcccaaaac cttgccgatg gccttaggtt tagccaaacc cacaataatg gaacagctcg
    18961 ttttgtatcg atgggcggtg cttttggagc actgggagcc gatttttcgt caattgcggt
    19021 taaccctgcc ggtgtagggg tatacaaatc aggcgaattc accattacac catcgttcaa
    19081 aacacaaaaa gtatcatcaa cctttttagg tgtatcggca tcggataaca agaataggat
    19141 taaccttgat aatttaggct ttgtaatgtc gttcaagcct tataaatccg aagataaggg
    19201 catcctggca tacaacttat catttggcta caacagaacc aacgattacc actctaacgc
    19261 aattacccta ggcgattaca ataactattc catcatggac tatttctcag ccattactaa
    19321 tggtaaatac accccatcgc agctagagcc aacaacaaac tataatccct ttaatacgtt
    19381 aggaggtgga gcatgggaat cgattatggc taggaacacc tggctgcttt acaaatatga
    19441 ctctagaaca ggcacctatg aacctgccat ttaccttcaa gattcagtta aatataagaa
    19501 tatttcaact actgaaggtg gttcgggaga gtttacaacc gcttttggtg ttaatatttc
    19561 aaataagttg tatttgggtg catcgttagg aataactgat tttaattaca catttaattc
    19621 aacctattct gaagatgcaa aagaaagcaa tccgtccagg ccaaacggcg ataggttcta
    19681 ctacatggat tacaaacagt atgtagaaac gaaaggctca ggcttcaacc tgaaaattgg
    19741 aggtatctat actccgatac ctgaagttcg cattgggcta tcgttccaca cccctacatt
    19801 ttatagtttc gacgatgcat tctcgtcatc gataattaca aacttcgaca caactggcac
    19861 agaggttatc ttccaaagca acagcccgtt gggtaggtat gattatcatt ttgaaacacc
    19921 tatgaagatt accggaagtg ttgccattat aattatgcaa aagggattgg ttagcattga
    19981 tattgagcat ctcgattact ctaccatgcg tttccgccgc ggtggcgatg gcgacacttt
    20041 ctgggattta aatagccaaa tgggaaacac ctttaagagt gtcacaaacc taaggtttgg
    20101 tggagagtat aaaattgggg atatcgctct aagaggaggt tatgcctact acccatcgcc
    20161 ctacaaatcg ggtttcctta acgacaaatc caaaattcag cagatttcag gtggtattgg
    20221 ctacagaact ggcaacgtat caattgattt tgcctaccta agaacattac aggatgttaa
    20281 atatgttttc tatgatttga atattcctag cgttaccaac aaggtgcgtg atagtagagt
    20341 tctgcttact tttggtttta gattttagtt gggttgcctt caaaacagca aagggcgcta
    20401 aaggcgccct ttgctgttta ttaattacaa tgatctaagc cattatttta ctttcaacaa
    20461 ttgatagaat ttcagcctcc atttgttgag ctttcttaac tatctcggca cctctactaa
    20521 tgaactcatt ggctttatcc ttatccttta ggcctgaagc attgattttg acgttaagga
    20581 aagcccccat taccgctgag cgtgcagcca aagcaccaac accagcatcg gaaacggagt
    20641 taggattacc atgttcagcc attgccttta taacctccat cgactcaaag caaaggttca
    20701 tgacgcggaa tggcacctcg atggcgtata gggtagcatc ctgtatggcc tttgttcggg
    20761 ctgccttttc ctcgtcggtt cccttgggta agccaaaggc ttccattatg cggttgaacg
    20821 cagcagtatc ctcatcaacc attttaataa gctgctccat gtaaaacttg cccttttcgg
    20881 cccagttgga aaattcttcc cagcgatcgt cccaaccggg tttgtgcgat gataggtttg
    20941 ctaccatggt agcaagtgca gcgccaaaag cgcccatggc tgccgatatg gaacctccgc
    21001 caggagcagg cgattcggat gcagtttcct gagtgaaacc ctcaagcgtt ctgtcaacca
    21061 attttttacc ggttttttcc tcaaggatat actcaatgat tttcttatcg ggatcaaatg
    21121 ggtaaagctc atccaaaccc attgatttta cagcaatctt tattatctcc ctatcaggaa
    21181 taccaagcga acgctgctgc tttcgcagga aatatttacc tgcctcaagc atggcgctaa
    21241 ggggaacaac cccaacaatc tcagagccgg taacccttaa gcctctttct gctgcttttt
    21301 tagatgcctc ctcaaaagcc acatgaatag gggtaacggt tatatctgtt aaattgattg
    21361 acacctgggc aattccatac tcatcaataa accatccaat tgctttgcaa gcctttaggg
    21421 atccgggcac ccatatctca ttgccgttct catcggtggc aattttaccc gtaacagggt
    21481 tgcccacacg cattgggcgt cctttttcac ggatatcaaa tgctaccgca ttagctcgtc
    21541 gggttgatgt ggtgttaagg ttaacattgt atgccacaag gaaattgcgt gctcccactg
    21601 caatagctcc agttttagga agaaattgtg ttgggccaaa atccggcttc caagcaggat
    21661 cctgaagttt cttgggtaat ccctcgtact cgcctgccct acaatttgca aggttacggc
    21721 gcttttcctc aaatgctgca tactcataac aataaacagg aattcctaat tcctcaccaa
    21781 tacgtttagc cagcttgcgg gcatactcaa ccacctcgtc catggtgatg ttggccaccg
    21841 gaacaagcgg gcagacatcg gttgcgccaa atcgtgggtg agcaccatgg tgcttgctca
    21901 tatcgatgag ttccattgcc ttccgggttg cccgaaatgc tgcctcaaca acctcatcgg
    21961 gggttcccac aaatgtaact acagttcggt ttgtagcttt gcccggatca acatcaatta
    22021 acttaacgcc ttcaacggct tcaatctcat cagttatctg cttaataatt gtcatatcgc
    22081 gcccttcgga aaaattgggc acacactcaa ttaacttttt cagtgacata atggttatgg
    22141 tgtttgttaa agttcaaagg ttctaggata aaggttaaag gttaaaggtt aaaggttaaa
    22201 ggataaaggt taaaggataa aggataaagg ataaaggata aaagattaaa gtttaatatc
    22261 ccttaattgt ttgtttagtt attcccgaaa actcgtttta gtatgtcagt tacccgggcg
    22321 gctggatcct tacggatttt taactcttcc tgctcaatta ggacaaaaag accatctata
    22381 gccttttggg tcacataatc gtcgagattt gggtttactg ctttacctcc agttaaaagg
    22441 gttagcctat tataggttgt tgctaaaggc tcccagtaag aggtgagctt taccttttga
    22501 atagcctcat gaacctgagg cttaaaggcg gcaaatagtt gctcagatgt gtttttccta
    22561 aagtattcgg tagccgcatt attagggccc ctcagtattc ccatggcgtc agtaaacgac
    22621 attttagtaa tagcgtcagt aaaaataggc aaagcctttt tggcagcctc ctcggcagcg
    22681 cgattaagcg ttagtgtaaa ctcatcgacc tgcttggaga aaccggcatc gaccagtgtt
    22741 ttgcgaacct tttcggcttc ggggggaaag gggataaaaa tcttggggtt tttgtaaaac
    22801 ccatcaacct gcgatgctaa acccgttgag tttttagcgc caacggctaa tgcctcttta
    22861 agcccttgaa ccacctcagc attggtaagt ggcttactcc ctgaaccttg cgggataggg
    22921 aaatttgtag gaattgatat ttcagcacat gccgatatca atatagctat tggaagtaat
    22981 aagttttttt tcatactcca actatttatc gttgataata ctgccattca ggataaccgt
    23041 ttcaatcaaa tcggatgtat aggcgtaggg catatactca tagctgggta ttggcttggt
    23101 aataaacacg tttgccacct tacccttgca aatgcttccg tgtgtatcgg aaatacccat
    23161 ggcgtatgca ccgttaattg ttgttgcatt tataacctcc tcgggcaaca tgcggagttt
    23221 gatacatccc agcgacatga taaacttcat atcgcccgat ggagaagaac cagggttaaa
    23281 atcggatgct aaagcaatgg gaagacccgc gttgatcatt ctccgtacgg gagggtcaac
    23341 catacccagg aaaaatgaag caccgggcag aagtgtaggc atagtttcag tgccaagtag
    23401 tgcattaatc tcatcatcgc cagtgaactc taaatgatcg accgagagcg cattatactt
    23461 tacgccaacc tgaataccac ctgaaaaatc gagttcgttt gcatggagtt tgggcctaag
    23521 gccatacttg attcctgcca tcaggatacg ctcagtatcc tcaacggtaa agaaaccttt
    23581 atcgcaaaaa acatcaatat aatcggctaa gccctcagat gcaaccaaag gtatcatctc
    23641 gttaataatc aaatcaacat actcactttg tcggccttta tactcagcag gtactgcgtg
    23701 agcgccaaga aaggttgatt ttatggttat aggcgtagtt tcctttaacc gtttaataac
    23761 ccgaagcatt ttcaactcac tctcggtgga taagccgtaa ccgctcttga tctccacggc
    23821 tccggttccc ttcctaatta tctcgtaaat gcgagcaagc gattggttgt aaagctcatt
    23881 ttcagatgtt tcggctaaac gtttggctga gtttagtata cctccaccac gtttagctat
    23941 ctcttcgtag gacagaccct ttatcttgtc gatatactct atctcgcggc taccagcata
    24001 aaccaaatgg gtgtgcgaat cgcagaacga tgggaaaacc atacggccaa acgcattgta
    24061 aattagcaca tcgcccgaaa gcgatgaaat atcgggagct ttggacattg gcccaaaatc
    24121 ggcaattaac cccttgtcta tcagtaaata tgcattatca atagttttca gcttggccat
    24181 atcgttaccg gctttccaaa ctgccggttt atcttccacc tgaaccaact ttttgatatt
    24241 tagaataagt atttgcatgg gcgaatgcat ttatatggcc tcgaagatag gcaattaaga
    24301 tgaattctga aatgttttta agcaggttgt tggggggagg gtaaaaaagg gttgtttacc
    24361 attattaagt ggaaatgggt cggaatgctc ggaatactcg gaatactcgg aatgagtcgg
    24421 aatgctcgga atgagtcgga ttgagtcgga ttgagtcgga atgagtagga atgagtcgga
    24481 atatggtgga gttagaagaa gttagaggaa gtttgaggaa gtttgaggaa gtttgagtta
    24541 gttaaaagtg aaaagtgaaa agtgaaaaga ggaattgtgg atatgagggg gttagagaga
    24601 gtttatgttc gttaatcgtt aatcgtgaaa cgtgaaacga aaggatttga cccgtcctga
    24661 aaaatatcta gtcctgaaat aagtctacag gttagaaata gatttgagcg acacaaatat
    24721 accgttaacc gttaaccgtc aacaatcaac tatcaactat caactatcaa ccaccaactg
    24781 tcaactatca actcaataat tttcaaagag ttgcacagag ttataaagag ttaaacattg
    24841 taattcaatc aaataattat catctgttac agtttaaatt cacggacaac aataaagcca
    24901 gtcgaaatga ctggctttat ttattttatt ttatctattt acctttcttt ttcctgagct
    24961 gttcctgctg ctttgccata tcctcgagtc tttgttgcca ttttgatttt gtaacgggtt
    25021 tcttggcatt ctcgtgaagt ttggcaagta tagccttatc atcgacaaag cggcggaaga
    25081 gataggtttg tcctatggtt attacgttgg atagcaagta gtagtaactt aagcctgccg
    25141 aatagttgtt gaaccacaac aacagcatta taggcatcat ccaggtcatc ataaacttca
    25201 taccgggcat ttgggcattg gtatcgccca tttggtcggt attcatcttt gaggtgatga
    25261 aaagcgaaac ggccatcaac agtgtgaata agctgacatg atcgccatac catgggatac
    25321 tgaaaggtaa gtgaagaata gaatcgtatg atgagaggtc gtcggcccaa aggaaacttt
    25381 gctgcctgag ctcaaatgag gcggggaaga aacggaacat ggcaattagg atggggaatt
    25441 ggattagaat gggaatacac ccacccatgg ggttaacccc tacctttttg taaagcgcca
    25501 taacagcctg ctgcttcttg agtgcgtctt cctgcttggg atactttgca tttatttcat
    25561 caacctgggg tttaagcacc ctcattttag cagaactcaa gtatgattta taggttaagg
    25621 gcgaaataat aatcttgata aatatggtta aaagtaaaat aatgagacca tagcttgaaa
    25681 tgtaccggct gaggaaatta aaaacattga taacaatata cttgtttatc caacgaataa
    25741 tattacggcc taatggaaca accttctcaa aacccgaatt gtatgacttt aaagttttat
    25801 agtgattggg tccaaagaag aatttcagac cgatactttc cgattcctta ttttggaaag
    25861 gcaaagccaa gcgggcgcta aactgcttaa catgctttcc atcgtcaata ttaactgcct
    25921 ttaaatcggc attaacaaaa tggtcgttgg ctacaatgat ggatgaaaaa aactgatgct
    25981 taaaatcgac ccatttaatc ttgttattca gctcctttga atcctctttg ctccgagagc
    26041 tgaactcctc ataatcacct gtaaaaaatt gataggcaat tgttgtgtag ttacgctcat
    26101 tctccaaacc cttttcaaga cgtagtaact ttgaatccca aataagatcg atgtaaccca
    26161 aacggttccc aatattcttt tccaggccag taaacttaat atcaaagccg agcatgtagc
    26221 taccgggctt aattgtataa atgtaatcga tataggaatt accacctgca gctagcctca
    26281 tggttactga aactgagtcg gtggtagccg taataatact atcggttgtt tgaggggtaa
    26341 agaataaatc gtttgtttta acggcaacat tatcgcccca gaactgaagg ccaaatgtat
    26401 tttcatcgcc agtaaagagc acgagcggtt cacccgaata tgttttatat ttcttgagtt
    26461 ctaccgagta aatacggccc cctttagctg taagggtaac cctcatcaga tcgttctcaa
    26521 gggtgataaa cttgttgctg ccgcttgccg catcggaaaa taatccgaac tgagtaatgg
    26581 cctcctgttt cttggcacta tcggaaagta aggctttagc ctgctcttcg gcaagtcgtt
    26641 tttggtgctc ctcctcctga acacgtgcaa ttgaatcggc tttccgttgg tatgcctcac
    26701 gctcctcctt tgatggttgt gtgaggtaac caaagccaat cattataaga aaaatcaaca
    26761 ctaaaccaat aaccgtattc ctatccatct caaaaatatt attcagttta aaatttaaaa
    26821 ttcaacaaat tggagtgagt gcaaaggtag catttacacc ttactttatt tgttaaaatt
    26881 tattgcagct ttcacaaaat caacaaataa ggggtgaggt ttaagaactg tgctgctata
    26941 ctcagggtga aactgaaccc caacaaacca agggtgatcg gacaactcca taatttcaac
    27001 aaggttagta tcggggttaa tgcccactgg tatcattccg gcattctgga attgctcaaa
    27061 gtacttatta ttgaactcat acctatgcct atggcgctcg ctaatctcaa gttttttgta
    27121 tgccttatgg gtatttgaac ccttggccac tacacatggg tatgcaccta aacgcatggt
    27181 tcctcccatg tcggttatgc ctttctggtc ttccattaga tcgataaccg ggtaaggcgt
    27241 agttcgaacc atttcggttg aattggcatc ggttaaccca aggacattcc tagcaaactc
    27301 aataactgca cactgcatac ccaagcatat tcccaggaag ggaactttgt tctcacgagc
    27361 atacttaacc gcaagaattt tcccctcaat acctctatgg ccaaaccctg gtgctaccag
    27421 aatgcccttt aaatgaccaa gtttctcgct aaggttacta gcgttaatat cctcggattg
    27481 aatgagttcc agctcaacct tgcagtcgtt ggcggccccg gcatgtatga acgattcaat
    27541 gattgacttg taagcatcag gtagttgaac gtacttacca accaatccaa ttttaacgcg
    27601 atggctggga ttcttaaccc ggttaacaaa gttaatccag tcgtcgagct tgggctccga
    27661 tgcaggaagg ttaagcttac ggagtatggt taaatccagt ttctccttga gcatcaatag
    27721 tggaacctcg tagatggttt taacatcaat tgactccact accgcctcgg gttcaacatt
    27781 acagaaaagg gctacctttc ggcggatatc gtcgctcaaa tgcttctcgg tgcggagcac
    27841 caacacatcg ggctggatac cgttctcgag caactccttt acggagtgtt gtgtgggttt
    27901 agtttttagt tcacccgatg cggaaaggaa aggaacaaga gtgaggtgaa taaaagctga
    27961 attatttggg cctaactccc atttaagctg acgtacagcc tcaatgtatg gcagcgactc
    28021 aatgtcgcca accgttccgc caatctcggt aataactaca tcaagcttat tttttgttcc
    28081 aagcagctta atccggcgct ttatttcatc ggtgatatga ggaataacct gaacggtttt
    28141 gccaaggtaa tcgccacgcc tctccttact tattaccgtt tggtatattt taccggtggt
    28201 aacattgtta agctgcgatg tgggcaaact gataaagcgc tcgtagtgcc cgaggtcaag
    28261 gtcggtttcg gctccatcct cagtaacgta gcactcgcca tgctcgtatg ggttgagtgt
    28321 tcctgggtca acatttatgt atgggtccaa cttctggatg gttacagtgt atcccctact
    28381 ctgtaaaagt ttacctaacg aagctgagat tatcccttta cccaacgagg aagcaactcc
    28441 tccggtaaca aaaatgtatt tggtagatga ggactgtggc atgaccttaa gtattactgt
    28501 tctaacttat caataagctt aattttttcg ctcagctcag caatttcctc ttttgcccgc
    28561 tcaattttac gtttaacctc ctcaatcatc gcctcagcat tcttggtttt agcaaagaaa
    28621 ccgatgttgt tctcccaaac ggtgaggtcg ttttccagct gacgcatgcg gttaaacatg
    28681 cgttccctat cctggcggag tttggacatg gcacgagggt tagccttagc ttcctcaagc
    28741 ttagcctgaa aacgcaccac cttattcttg ccctcctggg catttaactt atcgaaatgc
    28801 tttttgatgg cagcccggaa acgcttttgg acatcatcct ttaccttgat tggaacaaac
    28861 cctatttcgg tccagcgcct ctggaattcc ttgagggcgt taaggttttg ttccatatcc
    28921 tcaccaaact caaaggcttc aacctcggca atcaatgctt ccttggcctt gagattttcc
    28981 tcatatgagt tatcgaccga tgcaaaatgc ttcgactttt gctcaaaaaa gtaatcgcag
    29041 gcagcgcgga aacgcttcca aatttcatcg gagtttttac gaggaacagg acctatttgc
    29101 ttccagcgtt tttgaaggtt gattaaatct tccgtagttt ttttccagtc agtgctttcc
    29161 tttaatgcct cggcctgcag gcagagttcg gttttaagct gtaggttgtt cacctgaaca
    29221 tctctaatgt ccttgaaaaa gtcacgtttc ttgttgaaga aagtgtcgca ggcgctacgg
    29281 aaacgctcaa aaaccttatt gttttccttt ttggttgcaa atcctattgt tttccaaagc
    29341 ttttgcagct caattagttc ctttgatagt ttattccact cctttaccga ttttggattt
    29401 tgagcggcaa gttcctctgc cttttcgcat agctcagctt tggcttcaag attctttttt
    29461 tgctgctcct ttatgctttc gaaatgttcc tgatgctttt tgtttatttt agttgtagcc
    29521 tccttgaaac gctcccagat ctcattctga ttttcgcgtg gaactggccc aacctcgcgc
    29581 cactgctcat ggagtttctg caatttctta aatgcaacaa caaccgaggg ctcaagaagt
    29641 aactcctcag cctgctcaca gagtttgatt ttggcttcaa gatttttctt gaaatcgagg
    29701 tcgcggagtt ccttgctaat ctttacaaag tcgtagaagg cctgaacgtg atggtggtag
    29761 ttgttccaca aatcgtttaa gcttgcttgg ggaacagggc caacttcaag gaactgcttt
    29821 tgtaaatccc taaactcctg atagatttta ccaacaggct cggtgctgtt aaccagatcc
    29881 tttattttct ctataatttg ctgtttttgc tccaggttat gctgtttttg ctcctcgagc
    29941 tgattgttat actgagcgcg tttggcacga tactgcttaa gcagttcctt gaactgcatt
    30001 tcagcattat cctcgggcaa cgagaactgc tcaaggtcgc ctccggcgtc aacaaattct
    30061 tttcttaact tttcaacctc agccttatgc ttgcggtaga atatgctctt taaactctca
    30121 actttctgac ggattttttg aacgggtcgg ttattgatta aatccttcag ggtttgaacc
    30181 agctcatcct ttgaaaggtt tgagtagtcc acatggggct catcgtcttc ctcgtcaatc
    30241 tcggcctctt cctcatcctc ctcatcaaaa tcgacatgtt cgtcgtccat ttcaaggggt
    30301 ttcagctcct catcgttggg ctcctcatcg taagcctcat cggaaattga cataggtttg
    30361 tcagcattaa catcgaccaa atcgttcacg ctatcagcaa catcgtggtt gccggcattg
    30421 gaagatacaa tcgtaccctc gatgttttcg aggttttgat ccttaggatc gttcagctca
    30481 ttagtattca tagttcaaaa cttaaccggt tataaatcag cagtatttaa aaaattttgc
    30541 ctacgaaatt acatataatc gggcgtaacg caaacaaatt aacgtaaaag aataaagaat
    30601 aattgactgg caaaacaaaa aaagcccgat tttaaaggga ttcgggctga ataatatttg
    30661 caatataaat acttagtcaa ttttaatgct tctgggtttt tgtggcttag cctcatcgcg
    30721 cttggaaagg aaaaccgtga ggatgccatt ctcgtaacgg gcatcaatct tattggtatc
    30781 aatagccttt ccaacaacaa aggttcgctc aaatgagcct gttaggaact cgcagcgggt
    30841 ataagcctca ccttcaacag gattattttc cttctcggcg ctaatcttaa gcatatcatt
    30901 atcgatactt accttaaaat cgtccttttg gtatccggga gcagcaacct ccaaacgaaa
    30961 gcgctctggc tcctcataga tatttacggc tggatagctg agcctggcat tagccatttc
    31021 gctggccgat ttaacaaagc tttcgaacac cctctcaaaa tccacccctg tcctccttga
    31081 ggcaagcggg ttgtagaaac ttacaagtgt catagtatta taatttttaa agttgatatt
    31141 cacattcaca tttaatattt agctaatagt atgccataca gaaaaacaag tcattatgtc
    31201 atataaatac aaaattacta tgtcaacatg acactaaaac acctcgggaa atcatgaata
    31261 cttttaataa catgcgcatt ttaatagcat gcgatttact tgtgggttga tattttaaag
    31321 taaacaatgc atgcagaatt cagagtaacc aatcccatac aacgtcgttt agttaaggtt
    31381 gtttgtcatt ttgagtcccg atataatagg gaaagactcc gctgaaacat ctcaacgatg
    31441 gatgaatata gatccttcgc tccccgctag ggcgggacag gccgctcagg atgacaaatg
    31501 aagttgttcg tgtaagttgt tcgttattcg tgggatacga cctcacccca gcccttttcc
    31561 ctgagaggga atgaagcgga agaagcaagc agaggcataa aagcctgacc cgacaatggg
    31621 tcgggggtgg aacgtggcgg tgggtctaga aagtgatacc atatgagcca cgctgcaggc
    31681 gtggcgaata gtatcgcgtg gcagttcca
//
LOCUS       DAOOWJ010000022        28802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1640_length_28802_cov_7.752392, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000022 DAOOWJ010000000
VERSION     DAOOWJ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 28802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28802
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1640_length_28802_cov_7.752392"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="PLW22_03795"
     CDS             complement(<1..1586)
                     /locus_tag="PLW22_03795"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOU73860.1"
                     /translation="MKCLGKAMVVLLVLVGYGISAQVPQRIAYQAVLRNADGTVMANQ
                     QVELQVLLHKTSATGDVVYSETHAKTTSNQGLVAFEIGAGTVNSGTFSGIIWSDPIFL
                     ELKVKKQGQQSFTNLGTSQIVAVPYAFLAEKSLNASNGISSLGPLGNTVFSNGQEWTP
                     TARVSVMDSTVIVSPGEGHDPDKPIFAITNSQGQVVMAVYETGVRFNVEGSSSKGAKG
                     GFAVGGLTNGKAPAPTYFQLEPSYAQFLFNQANITKGAKGGFAVGGLTGVKGDTLTYM
                     TIHPDSTRLFINDSPTKGAKGGFAVGGLTNGKSGNNIYMSLLPGNIRFAFDTTYTKGA
                     KGGFAVGGLTNAKSTSPNYFEVTPDSTYVVNTMVSYRDMVVAGNVTTNVGMPEPPVTD
                     ADGNIYRTVKIGSQVWMAENLKTTKYSDGLPINPNHFYKLITADSTTVFGNYYLDTAV
                     TVSGKNVCPDGWHVPTQPEWEAMFAFVSGTDWTFNSANLGKKIIDPYRDVYAGIYNWI
                     PNQGVLFATNETGFSARGAG"
     gene            complement(1955..3079)
                     /locus_tag="PLW22_03800"
     CDS             complement(1955..3079)
                     /locus_tag="PLW22_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOU73861.1"
                     /translation="MNKGILELIVIMLIALPVGILTIRYFFKNSILFLMGALTVTWGV
                     LIDVMVNLKYIYPEIFPAYITTPVVIVAGIWVMWFIARKVRKPLDQAIDDLHRISDGD
                     LTVKVNPEFVQHNDELGRLSKSVGNLSDKLREVIDGISQAAGELESSASQLSMSATSL
                     SEVTSEQASSLEEISSAMEEILASIQQNSENAMQTERIASSTSKKLEEGVESTNVALD
                     SMNEIAQKIGIINDIAFQTNLLALNAAVEAARAGEHGKGFAVVAAEVRRLAERSRQAA
                     NEIIAVSQRGAEISGKAKDIMNQNVHEIVRTTDLIREITAATHEQRTGTEQVNTSVQE
                     LNNTTQQNASLSEEVAASAEELNARAKALTDLIGYFRIRK"
     gene            complement(3156..3977)
                     /locus_tag="PLW22_03805"
     CDS             complement(3156..3977)
                     /locus_tag="PLW22_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73862.1"
                     /translation="MIYIATVHWETDKWIDIQLKFFKRNINQPYRVFAFLSGDACKHK
                     DKFYFACCEPITEHAIKLNLLARFISLQANDDDILIFIDGDAFPVKPIDSFVTLTLKQ
                     YPLAAIRRTENIGDIQPHPSFCFTTVGFWKSIEGDWKEGYEWETTAGIHRTDVGGNLF
                     KILIDLKIDWLPLERTSQLTNHGLMFGIYADLVYHHGAGFREPLSMYDRTKSLVGIVK
                     PRFIAKLLDLLLCNVSVKNRFRIHRLLGIGDRINYQNQRLSQSIHEKIAMDEFFV"
     gene            4040..5212
                     /locus_tag="PLW22_03810"
     CDS             4040..5212
                     /locus_tag="PLW22_03810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU73863.1"
                     /translation="MANPIQSPAVLIFTAETFPAMAGDGRNALLVASKLNENGYCVKL
                     ICLNPNGKLPKKEKLNGVEISRINYWYRTIIGRFALRLSLFRYLTINCNSSSKWIIFG
                     AMPGYKLIALVSTLKGVDCVFRSTLWGFDDAESVAGSLHRFYTRYLLSRLYGYYALNS
                     AFANSWRNVFGSFNILLSVQGVDLKKFDIDHKNGIRHEHRGKLQIAEGKFVILMVGHL
                     IERKGFPEIADWLSRIDEDFLLLHIGSTEAPEWDTMSRYNVGMKNLKKYVEEKLGDRV
                     RFMGRQNVTTQFYLLSDILLVASHAEGFPPNSVNEALAAGLPVLTLRMPGVADVITEG
                     VNGFVFTDESEFARKLSILINNPVRRNELGRNARVFALDKLDINKIIPTLQNFLGL"
     gene            6225..6929
                     /locus_tag="PLW22_03815"
     CDS             6225..6929
                     /locus_tag="PLW22_03815"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOU73864.1"
                     /translation="MRLGILALLLIAATVTSTTISAQETRNFDAKGFDGIRNSTSAEV
                     QISQSPNYGIEVTAKSEVFNELEIVVKENVLHIKSKDNLRFSSNWGKVLVKVSCPDIK
                     LLAQNGSGNMIALTPIKGADEFTTSVNGSGDILLKEIQANSVQVSINGSGNIKTSAQG
                     KTYTLKANSNGSGNIDLETLTSEIAQLKLNGSGDATVRCNKEFKGSINGSGSITLYGN
                     PLIDAKVNGSGRLVRK"
     gene            7130..7549
                     /locus_tag="PLW22_03820"
     CDS             7130..7549
                     /locus_tag="PLW22_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73865.1"
                     /translation="MLRVAVDFDGTIVENEYPQLGKPMLFAFETLKELQKRGFVLILW
                     TVRTGKLLEEAVEFCRQNGVEFYAVNRNHPEEILDPETPRKLDVDVFIDDRNLGGFPG
                     WDKVFSMLCPNEPYEVSQKKVLKQYKTKSFVRRIFGI"
     gene            7560..8636
                     /locus_tag="PLW22_03825"
     CDS             7560..8636
                     /locus_tag="PLW22_03825"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOU73866.1"
                     /translation="MLQRISILLLVLAVACTNGRKDQLPDTTSMPAENPLHLIRFDKP
                     TSGSLFTAGESIDITIKLTDTIQPDSIVLYRNSERIQKVSNLSFTLQTQAGKPGTIQL
                     NATAWKDGRRQTASISIKLKSNTKPASYSYRIVKAYPHDPEAYTQGLFYHNGYLYEGT
                     GQQGASSLRKVELETGKILQSVNLSREYFGEGIAILDDKIYQLTWTNGTGFVYDAATF
                     KPLNTFSYTTQGWGLTTNGNELIMSDGSNTIYFREPSGFSELRRIEVFDNNGPVKMLN
                     ELEYIDGKIYANVYLTDRIVIINPETGVVEGNIDMQGLLTTTQRTGKEDVLNGIAYDP
                     NGKRIFVTGKLWGKLFQVEFIKKK"
     gene            8637..10079
                     /locus_tag="PLW22_03830"
     CDS             8637..10079
                     /locus_tag="PLW22_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOU73867.1"
                     /translation="MSTRTETDYLGSLQIPADALYGIHSVRAKDNFPDTTQFHVEWYQ
                     AMGLVKQACYLTYAEMVKALQEENLFRDSKGFIPPNVVNALIAVAGEVHEGKYFDSFI
                     VPAIQGGAGTSINMNVNEIIANATLIRLGHNPGNYTVIDPIEQANVYQSTNDTVPTAL
                     RVAVVRLLTLLEELINNTRQAVETIEKSYRNSLRIGYTQLQAAVPSSYGQLFSTYSEA
                     LSRDWWRVSKCFERIKVVNLGGGAIGTAVGTPRYFVMEVVKHLQQLTGFPFTRSENLN
                     DATANLDSFVEVHAILKAHAVNIEKMASDIRLLASDIAGNAINIPKRQVGSSIMPGKV
                     NPVIPEFVVSIAHRVYTNDMLISNLAGQGALDLNAYLPTIGHAMIDSIKLLIAANQAL
                     AGHLLNGIEIDSEKSAQLLMFNPSITTALLPIIGFNRAAMLAQEMRLKKIDIFSANQN
                     LKLIPEEKLVELVKPNNLLKLGFSFREVID"
     gene            10161..12434
                     /locus_tag="PLW22_03835"
     CDS             10161..12434
                     /locus_tag="PLW22_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73868.1"
                     /translation="MKKFTEYIVVVAVGLLFTYLFAGDVVKNINHRYFAQSGDGAKDY
                     YATFYHIKHDKSYIHSNSMNYPFGESIFFAGSQPIVSNTIKFISQNIYDVSPYTVAIY
                     NLLILISIAFTVLVIYLIFKHLNLPIWYSLLVAIGITFLSPQIDRIGGHFALSYTIVL
                     PLFILLLLKFDRQKSIGLSVLILFAGMWASITHGYFFTFYMVMFLAYWIWQKPWRKEF
                     EWRKQWLHVVLQIAIPLIVVPTLILYIEPVEDRTTWPWGFLFYRAFPESVFLSPGRPY
                     WGWLNSLVKIRNLQWEGIAYVGLVSTIIFLIGLFSFIKNAVNRRWGKTLTITSQPLMD
                     RLFWLSFLLLLFSFGIPFIFKLEFLLEYLGPIRQFRGIGRFAWLFYYTMNIVAFYLIW
                     QKVKGYKPIYRILLVIPIAILLYDACFHSKSQKHFINNSLPELDYAFHSNSKPNINID
                     EFQAIMPIPFFSVGSENYWHESACFGSMVKMYALSQLTGLPLNATYTSRSSVWRCVEN
                     LNLIMEPLQEYPVLKYYNHGKDILLFVDPHCDQINQNERRIITISTPIDTLWGYELRR
                     LKVADLAKLPEVYRNEYLNATPAMADTISGNYYFQEFESTPTPIAFKGNGALKINRRG
                     YTNLYWGRLPNNSSSEITVSFWVNRMKADIVPRNRIEIVTGKEEGKWETWNGFGFMEK
                     TKAFWNDWALVEYNLKLNNPSDFICISVAPLLAKSGDVYIDNVLIKPSNFNVKFVMGD
                     FVLLNNRLFNNISIGSD"
     gene            complement(12431..14479)
                     /locus_tag="PLW22_03840"
     CDS             complement(12431..14479)
                     /locus_tag="PLW22_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU73869.1"
                     /translation="MKAKHLLLSLFVFVALVSNAQLVTDPQQVVADILEEMAANSDTE
                     QDYSELVEDLLQLAESPLNLNAARKSDLQKLFFLTDFQIESLLSYRDSTGKILSVYEL
                     QLVPGFDLTDVERLAPFVVAGEVVKPLKVSDFTMGRHDIFTRLKTTLQTPVGYRSDYG
                     GASSYLGSKYAIYTRYRYRANRLQIGVTAENDAGEPLLDGTFPSGFDYLAGYAMVSDV
                     GKVKRLVVGDFRAEFGQGLTFWNSLTFGKSANVMGLHKRGRGIVPHSSAYESLFLRGA
                     GFTLNFKHIDFSLLASYQKIDGNLVDTVSSGELAFSSLPETGYHRTASEIASRRNIPE
                     LVTGSNITLTLNKIRLGTTAVYSRIYGENLNNQPIYSLTPASTEKLAMGINADYYYRQ
                     HLFFGEIAIDIFSQKMAALAGGQLKLSNRVHLSVLGRSYSTTFNPRYTAALAEGSGAT
                     NEKGLLMGLSLLAAKGWKISGYIDLYQFPWMRYGVYSPSAGRDFLFLSEHSLNTYFQL
                     SFRYRYKQDEKNMTGANIPVTSTLTQTRQGFRTQLTYQPNPLVNLRSTLEISNYRTDS
                     LSGGEYGYLLAQDININLQKTPLSIRLRFAIFDTESWNTRLYSYESDLLYSFTVPAYY
                     SNGTRIMAMLKYSPSKGVDLWLRYAQTYFPGSATIGSGPDLIMGNTRSEVKCMVRVKF
                     "
     gene            complement(14476..15318)
                     /locus_tag="PLW22_03845"
     CDS             complement(14476..15318)
                     /locus_tag="PLW22_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73870.1"
                     /translation="MEIFRQFSIVLLLLLQNLSVYCQADIRPVSGAAAAMGNCSLSMQ
                     GNWDGFTNQASLAYQRQFWVGAHHENRFMLSELGLSMLGFAIPVKPGTFGIDISHLGF
                     SGFSTSRAGLGYGMFLGKRFAAGVGLALTRMQLPADYRNATAFTVEGGVHYMPTSKLT
                     FGFYVFNPTGSTFDDLGDLPTLMGLGVTYLPSQNILLAFQIDDDTQRAPTFRFGLEYR
                     VMERTALRLGYSAASPEGITAGVGLPVKGFDIDFSLSHHQQLGFTPSLSVAYLFGKKD
                     NKTQ"
     gene            15472..16011
                     /gene="hpt"
                     /locus_tag="PLW22_03850"
     CDS             15472..16011
                     /gene="hpt"
                     /locus_tag="PLW22_03850"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOU73871.1"
                     /translation="MKQVKLKDKEFRLSYPETEIQNDIDLVASKINHDFAKSGEVPFF
                     VSVLNGSFMFTADLLKRITVPCEVSFVKLASYQGTSSTGKVNELIGLNENVTGRTLVV
                     LEDIVDTGITLSKLYEELRKLNPKEIRVATLLFKPEAYKGNIKLDYIGRSIPNDFIVG
                     YGLDYDGLGRNLPDIYTLI"
     gene            16162..17202
                     /locus_tag="PLW22_03855"
     CDS             16162..17202
                     /locus_tag="PLW22_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOU73872.1"
                     /translation="MDSLVNSHFIIPFLISMFLAINMGGSGTGPAFSVAFGANVLKKT
                     SVPALFGLMVFLGAIIAGKATSATVGKGLVNPELMNYAQVSIILFAVSISLLVANLFG
                     IPQSTSQSTVLAVIAPGIYFHELNSRKVFFEVIPTWFILPIIAFILSLIIGKYIYTPL
                     RRRGYTISSRINDHYMLRWAIIFASLYVAFSIGANNVANASGPLTSMTMNELGLGNQG
                     GRLLILIMIMSTMIVAPSFGIGSSLFGEKILRNTGKELFLFGRIEAVIIAIISATLLL
                     AASLFKGIPTSLVQLNVGAILGIGVARLGPRNIFRKTAVRKFFLMWLIAPIIAFLISW
                     YLTFLADKYGYL"
     gene            complement(17298..18455)
                     /gene="dnaJ"
                     /locus_tag="PLW22_03860"
     CDS             complement(17298..18455)
                     /gene="dnaJ"
                     /locus_tag="PLW22_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOU73873.1"
                     /translation="MTTKRDYYEILGVPRNASKDEIKKAYRKMALKYHPDKNPGDKDA
                     EEKFKEAAEAYEVLSDDNKRARYDQFGHAGMGNGAGGGGFSHDWTIEDIFAQFGDIFG
                     GHFGSFGGFGGFGGGRSSRRTNRGTDLRVKVKLSLSDIANGVEKKIKVNKYVSCQSCG
                     GTGAAGGSSYSTCSTCRGSGYVTRVTNTLLGQMQTTSPCPTCNGEGKVITNKCVACNG
                     EGVVREDEVITVKIPAGVAEGMQLNVQAKGNAARRGGVPGDLLVMIEEEKHPNLVRDG
                     NDLIYNLNISIPDAVLGAPAEIPTVDGKVKIKIEPGTQPGKILRLRGKGLPEVNSYQR
                     GDLLVAVNVYIPKNLTREEKAIFEKFAKSPSFTPNPDEGGSFFERMRGMFE"
     gene            complement(18468..19061)
                     /gene="grpE"
                     /locus_tag="PLW22_03865"
     CDS             complement(18468..19061)
                     /gene="grpE"
                     /locus_tag="PLW22_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOU73874.1"
                     /translation="MSKKHKKEDIEQEQVNQQTTSTENNVTDSNTQAPQGDAQQTEAS
                     QANDDKVAELTTQLEEMKDKYLRLTAEFDNYRKRTLKEKAELIKFASEEVLKDLLPVI
                     DDLDRALKAIETANDINAVKEGISLIVNKFNDFLKAKGVKEIDAIGKELDTDLHEAIT
                     KIPVQDDAQKGKIVDVIQKGYMLHEKVMRFSKVVVGE"
     gene            complement(19290..21008)
                     /gene="recJ"
                     /locus_tag="PLW22_03870"
     CDS             complement(19290..21008)
                     /gene="recJ"
                     /locus_tag="PLW22_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOU73875.1"
                     /translation="MEKRWVLKDPGDPVLVSSLAKQINVNETIASLLVQRGVDTYEKA
                     KRFFRPSLADLYDPFLMKDMAEAVERIEQAIRRGESILFYGDYDVDGTTAVALMYSFF
                     RKRYSKIGYYIPDRYNEGYGISFKGIDFAFENGYSLIIALDCGIKAVEKVKYAKLRNI
                     DFIICDHHLPGERIPEAVAVLDPKRSDCMYPFKELSGCGVGFKLLQGYCKKEGISEEE
                     LYQHLDLLAVSIASDIVPIVDENRVLAFYGLEKLNTNPSRGLKSIIKIAGIENQRIAI
                     DDIVFRIGPRINAAGRMESGKTAVDLLCASDDSSAFEVGALIDSCNNDRKHVDRTITH
                     EALRMIAQDKRLQHSYSTVLFNPSWHKGVVGIVASRLIETYYRPTIILTQSGDLATGS
                     ARSVQGYDLYQAIEACSDLLENFGGHMYAAGLTMKVDRVEEFKRRFEEHVSKTITPEM
                     LSPQIDIDTRIRLDDITERFFNVLKQFQPFGPGNMSPVFMAENVYDNGEARLVGSEKE
                     HLKLTLVQEGSTQKFDAIAFQMAEYYKPIHYGQPFDACFAIHENNYKGNITLQLRVKD
                     IKLRAK"
     gene            complement(21027..21719)
                     /gene="lipB"
                     /locus_tag="PLW22_03875"
     CDS             complement(21027..21719)
                     /gene="lipB"
                     /locus_tag="PLW22_03875"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOU73876.1"
                     /translation="MQNVKFHDLGTVDYKEAWDYQEKLFDEVTKRKLNGDGKLHYLLF
                     CEHPHVYTLGKSGENANLLIPEEMLKRINASFYHINRGGDITYHGPGQIVAYPIFNLE
                     AFGLTLKSYIHLLEQVVIDCLQEYGIVADRLEGATGVWLDPGVKGRERKICAIGVRAS
                     RYVTMHGLAFNVNTNLDYFRYINPCGFIDKGVTSMQVELNRQLDMEEVKAKLLNHFAH
                     RFHFACNRVGTV"
     gene            complement(21769..22635)
                     /gene="lipA"
                     /locus_tag="PLW22_03880"
     CDS             complement(21769..22635)
                     /gene="lipA"
                     /locus_tag="PLW22_03880"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOU73877.1"
                     /translation="MGNERKPEWLKIKLPGGEGYAQVNSIVKKHGLHTICSSGMCPNI
                     GECWGNGVATLMILGDICTRSCKFCATATGKPLPPDPNEPTKVARTVSLMGLSYCVIT
                     SVDRDDLPDHGAGHWRSTVMEIRRVNPNTTIEVLIPDFDGRPELIDLFLESEPDVVGH
                     NLETVERLTPLVRTKASYRRSLKVLEHIAKRGFRAKSGIMLGLGETRDEVIQTLDDLL
                     AVGCKMVTIGQYLQPRPTNLPVQRYITPEEFEEYKQIALAKGFEFAESGPLVRSSYHA
                     DKAQHLVKGCCK"
     gene            complement(22978..23613)
                     /locus_tag="PLW22_03885"
     CDS             complement(22978..23613)
                     /locus_tag="PLW22_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOU73878.1"
                     /translation="MSRYIAGLISQGEGLTLDFKHSITDSRKIARSLVAFANTAGGTL
                     LIGVKDNGNITGVNTDEEYYMVEAASQLYCKPEVPFEVVKWEINGKTVLEVKVKPSRR
                     KPHKAPDKTGVYKAYIRVNDENVVASPVQIKIWKAEKYRKPINILFGEPENALIDYLK
                     SHQTITIDHFSRLGFISVSEAEKILINLTAMGLIEYSNLNNEDHFSLRMAE"
     gene            complement(23655..24212)
                     /locus_tag="PLW22_03890"
     CDS             complement(23655..24212)
                     /locus_tag="PLW22_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOU73879.1"
                     /translation="MQKVIVAVTGASGAVYAARVIEKLIELSARVDEVAVVFSENGKR
                     VWEYELNRDITFGFPVKLYDNNDMFAPMASGSAGYNAMVVVPCTMGTLGRIASGTSND
                     LIVRAADVMLKERRKLVLVPRELPYNLIHLQNMVTVTQAGAVVLPASPSFYSRPSNMD
                     ELIDTVSDRIVEMLGFAIEHYRWGH"
     gene            24507..26789
                     /gene="rnr"
                     /locus_tag="PLW22_03895"
     CDS             24507..26789
                     /gene="rnr"
                     /locus_tag="PLW22_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOU73880.1"
                     /translation="MTKKKNKTDAGSKPNRIAKKELKKLVTDLLLANPTNIYNYKQVS
                     NKIGVSDDYTRRMVNEILYELALDGFAEELQRGKFRAVMQNALAVGILEMNPDGSADV
                     ITDDNREIFVPDFRLNHALHGDKVQISIYRQRRRGLLEGEVVRVIERAKRNFVGTINF
                     VGKNAFVVPDNKLMPYDIFIPKVELKDVRNGQKVIVQITDWPAREKNPNGKIVEVLGD
                     PGVHEVEMHAILAEYELPYRFPEELDILAEKISDKITEADYRERRDFRNVPTFTIDPF
                     DAKDFDDALSVQTLPNGNFEVGVHIADVTHYVKPNTPIDNEAIERGTSVYLVDRCVPM
                     LPERLSNYICSLRPNEEKLCFSAVFELNENAEVLNQWFGRTVILSKRRFNYEEAQQVI
                     ETGKGDMSEQILLLHKLAQKLRAERFKKGAIAFERVEVKFDLDTKGKPLGVYFKENKE
                     SNQLIEEFMLLANRRVAEFIGMRKDGRNELPFVYRIHDKPNEEKLNAFRSFITRFGYS
                     ISGTTDKQVSKSLNQLMEKVKGRPEQNLIETLALRSMAKARYSTDNIGHYGLAFRYYT
                     HFTSPIRRYPDMMVHRLLAHYLNNGKPKDREQIEMLCKHSTNMEIKATEAERASIKYK
                     QVEFMQDKLGETFTGVISGVTNFGIFVELTDSQCEGLVSIRDLDDDYYRFDEEEYALV
                     GQRTGRKFTLGDEVEVEVWRTNLAKKQLDFKLVGMKGRSSKPLKPRRSQTKSSPKNPK
                     VASSRKGQQKAKKKEKRRKR"
     gene            26803..28029
                     /locus_tag="PLW22_03900"
     CDS             26803..28029
                     /locus_tag="PLW22_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU73881.1"
                     /translation="MKRFIVIALAWAAFACSTKNELTPGSWLGVIQMDSIPGRLDVPF
                     NFEVKVDNDKVQLTVRNADELIEITEVERIGDSLYAKFPTFTSELVMAVNDSLGGYYY
                     PKGVKDGRRYKFYGIKGETDRFPWFTEQPKFDITGRWWFIENPGTPDSTVMVAELKQE
                     GSRVTGTILSTTGDYRYLEGKVAGNMFYFSKCDGAQSIIVRGKITDSATMVNGLISGS
                     PRWASAWRALRDDKAQLPEAQSLVWVRKGYTTFEFAGTDLNGSRVTSNDERFKGKVLA
                     VLAGGSWCPNCLDEGRFYKTMYEKYRDKGFEVVSLCFEDKTFEAAQPKIKRFAQSIGA
                     SYTFLYVAPRGREQRDSVLYALEGQMAYPTSMILDRNGKIRRVETGFSGPGTGEHYQK
                     FAHETEELIVKLLEEK"
     gene            28092..28727
                     /locus_tag="PLW22_03905"
     CDS             28092..28727
                     /locus_tag="PLW22_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOU73882.1"
                     /translation="MNDLLAFGLLGFTSFFTLINPLGAMPIFMTMTAELNPAERIKTA
                     RKASIVAFLTILFFAFTGQLLFRFFGISVNSLRVVGGVIFFMMGMDMLQARLTRVKIA
                     ENEVKKYVNDISITPLAIPMICGPGAITNAIILMQDATTIDKKMVLVFSIFLVMLLTF
                     LILYSSSKIIKALGETGNNVLMRLMGLIVMVIAVEFFFAGIKPVLIEIFGK"
BASE COUNT         8038 a         6518 c         6206 g         8040 t
ORIGIN      
        1 cttccagcac cacgggctga aaatcctgtt tcatttgttg caaataatac accttgattg
       61 ggaatccagt tgtaaattcc agcataaaca tcacggtagg ggtcaattat cttctttcca
      121 agattggctg aattaaaagt ccaatcagta ccagatacga aagcgaacat ggcctcccat
      181 tcaggttgcg ttggcacatg ccaaccatcg gggcaaacat ttttaccgct aactgttact
      241 gcagtatcca agtagtagtt tccaaacacg gttgtagagt cagcggttat taatttataa
      301 aagtgattgg gattaattgg taaaccatca gagtacttgg tggtttttag gttttcggcc
      361 atccaaacct gtgagccaat tttaaccgta cggtaaatat ttccatcagc atcagtaact
      421 ggaggttcag gcatacccac attggtagtt acgttcccgg ctactaccat atctctatac
      481 gataccatag tgttaacaac gtaggtacta tcaggggtta cttcaaagta attagggctg
      541 gtagacttag cattagttaa acccccaaca gcaaatccac ccttagcgcc tttggtgtag
      601 gtggtatcaa atgcaaacct gatgtttcct ggtaataacg acatgtaaat attgttcccc
      661 gatttcccat tggtgaggcc tccaactgca aagccacctt tagccccttt tgttgggcta
      721 tcattaataa ataaccttgt gctgtcgggg tgtatggtca tgtaggtaag ggtatcgccc
      781 ttaacgccgg ttagcccacc taccgcaaag ccgcctttgg ctcccttggt tatgtttgcc
      841 tggttaaata ggaactgggc gtaggagggc tctagctgga agtaggtggg tgcaggtgct
      901 ttaccattgg ttagtccacc aacggcaaat cctccttttg cacccttact gctactaccc
      961 tcaacattaa atctcactcc agtttcgtaa actgccatta caacctgtcc ctgcgaattg
     1021 gttatggcaa aaatcggttt gtcgggatcg tggccttcac cgggagatac aattaccgta
     1081 ctgtccataa cgcttaccct tgcggtggga gtccattcct gcccatttga aaatacagta
     1141 ttacccaaag gccccaaact ggatataccg ttgctggcgt tcaatgattt ttctgctaaa
     1201 aaggcgtagg gtactgctac aatctggctt gttcccaaat ttgtaaaact ttgctgcccc
     1261 tgctttttaa cctttaactc caggaaaatg gggtcgctcc agatgatacc actgaatgtt
     1321 cccgaattga ctgtgcccgc accaatctca aatgcaacca gtccctgatt ggaagtggtt
     1381 ttggcgtggg tttcagagta cacaacatcg ccggtagccg atgttttatg tagtagcacc
     1441 tgtagctcaa cctgctggtt tgccattacg gttccatcgg cattccgcag gactgcctga
     1501 taggctatcc gttggggtac ctgtgcgcta attccatagc ctactagcac cagtagtacc
     1561 accattgcct ttcctaaaca tttcatcttt gtatagtttt tagttttaag tattaaagaa
     1621 cacaagttat atttttaaat actacttgtc aattatttta tgataatttg atattaattt
     1681 ttcattctta attttaaagt tttatgagcc attaaaaacg tgaaagattt cccctgtgta
     1741 tgcattactt gcccattagt tataaacatt cagaattatt gttttggcat caatgagatg
     1801 tcattaattt agaatgattc ttaacatgta gaaacaaagc aagtaaatac attgcagata
     1861 cagttaaact ctttatattc ggctgttgtc tattaaaaat ctattaatct aattttgacg
     1921 aatggccgtt ctggtttgat gaatgaaatt ggtgctactt ccttatcctg aaataaccaa
     1981 tcaaatcggt taaggctttt gcccgggcat taagttcctc ggcgctggct gccacctcct
     2041 ccgatagtga ggcgttttgc tgggtggtgt tgttaagctc ctgtaccgag gtgttcacct
     2101 gctcagttcc ggtacgctgc tcgtgggtgg ctgcggtaat ctcccggatt agatcggtgg
     2161 ttcgaactat ctcatgaaca ttctgattca tgatatcctt ggccttaccc gatatctccg
     2221 ctccccgttg cgataccgca ataatctcgt tggcggcctg gcggcttcgc tcggccaagc
     2281 gccttacctc ggccgccacc acggcaaagc ccttcccatg ctcaccagcg cgggctgcct
     2341 ctacggctgc gttcaacgcc agcaggttgg tctggaatgc aatatcattg atgattccta
     2401 tcttttgggc tatctcgttc atggagtcca gcgctacgtt ggtggactca accccctcct
     2461 ccaacttctt ggaagtggac gaggctatac gttctgtctg catggcattc tcggagttct
     2521 gctgtatgct ggccaggatc tcctccatgg cgctggatat ctcctccaac gatgaggcct
     2581 gctccgaggt aacctccgat aggctggtgg cactcatgct cagctggctt gccgacgact
     2641 ccagctctcc tgcggcctgt gaaatgccat caatcacctc gcgtaactta tcggatagat
     2701 tacctaccga tttacttaac ctcccaagct catcgttgtg ctggacaaac tcagggttta
     2761 cctttacggt caaatctcca tcacttatcc tatgtaaatc gtcaatagcc tggtccaaag
     2821 gcttccgaac cttgcgggct ataaaccaca tcacccaaat acctgctact ataactacag
     2881 gtgtagttat atatgcagga aaaatttctg ggtagatgta ctttaagttc accataacat
     2941 caattagaac tccccaggtt acagttagag ctcccattaa aaagagtatg gagttcttga
     3001 aaaagtacct tatggtgagt atgcccacag gtaatgcaat tagcattatt acaatgagtt
     3061 caagtatgcc cttattcatg gctgaaaggt tttatgttca acatttgcta tttagtgggc
     3121 aatataaaat aattgatggg ttaagtcaag gaaaactaca cgaaaaattc atccattgca
     3181 atcttttcat gtattgattg gcttaaacgt tggttttgat agttaattcg atccccaata
     3241 cccagtaatc tgtgtatcct gaaccggttt ttaaccgaaa cattacatag tagcaaatcg
     3301 agaagttttg ctataaacct tggtttaact attcctacca atgatttagt cctatcgtac
     3361 atggacaagg gttccctaaa accagcacca tggtgataaa ccaaatcggc gtaaatacca
     3421 aacattaagc catggttagt tagctgcgat gtgcgttcaa gtggtagcca gtcaattttt
     3481 aggtcgatta gaattttaaa gagatttccc cctacatccg ttcggtgtat accagcagtg
     3541 gtttcccact cgtaaccctc cttccagtcg ccttcaattg atttccagaa ccctaccgtg
     3601 gtaaaacaga acgaagggtg tggttgaata tcaccaatat tttcggttcg ccttatggcg
     3661 gcaagcgggt actgttttaa cgttaaggtg acgaaactat caatgggttt aacaggaaag
     3721 gcatcgccat caataaaaat aaggatatcg tcatcgttag cttgaagtga aataaaccgg
     3781 gctagaagat ttaacttaat ggcatgttcg gtaatgggtt cgcagcaggc aaagtagaac
     3841 ttatccttat gtttacaggc atcgccactc agaaacgcaa aaactctata tggctggtta
     3901 atgttccttt taaaaaattt gagttgaatg tcgatccatt tatcggtttc ccagtggaca
     3961 gttgcaatat aaatcatacc tactgctttc ttatatcaca aaaataacca aatttgcttt
     4021 aactatccat aatggtaaaa tggcaaatcc cattcaaagc ccagcggttt taatatttac
     4081 tgccgaaacg ttcccggcga tggccggcga tggccgtaat gccctgcttg tagcatcaaa
     4141 actcaatgag aatggctact gtgtaaagtt aatctgcctt aaccctaatg gaaaactacc
     4201 caagaaggaa aaattgaatg gggtcgaaat atcccgaata aattattggt accgtacaat
     4261 tattggaaga tttgccttaa ggttaagcct tttccgatac ttaaccatca attgtaatag
     4321 tagttccaaa tggataattt ttggggcgat gcccgggtat aaactcattg ccttagtctc
     4381 caccctaaaa ggggttgatt gtgttttccg ctctacacta tggggttttg atgatgccga
     4441 gagtgttgca ggaagtttac atcggttcta cacccgatat ttattgagta ggctttacgg
     4501 ctactatgcc cttaactcgg catttgcaaa ttcatggaga aacgtttttg gtagttttaa
     4561 cattttgctg tcagttcagg gtgtagattt aaaaaagttc gatatcgatc ataaaaatgg
     4621 gattcgccat gagcatcgtg gcaaacttca aattgccgag ggtaagtttg taatactaat
     4681 ggtgggacat ctgattgaac gaaaaggctt tcctgaaatt gcggattggc ttagtagaat
     4741 tgatgaggat tttcttctat tgcacattgg cagcactgag gcccccgaat gggataccat
     4801 gagcaggtat aacgtgggga tgaaaaatct aaaaaaatac gttgaagaaa aattaggcga
     4861 tagggttcgg ttcatgggcc gacaaaacgt tacaacacaa ttctacctat tgtccgatat
     4921 ccttttggtg gcatcgcatg cggaaggatt cccgccaaac tcggttaacg aggcattagc
     4981 agcaggcctt ccagtgctta ccctgcgaat gccaggcgtt gccgatgtta ttactgaagg
     5041 tgttaatggc tttgtattta ccgatgaatc tgagtttgcc cgtaaactat caattctcat
     5101 taataacccc gttaggcgca atgaacttgg gcggaatgcc cgcgtttttg ctctggataa
     5161 gcttgatata aataaaataa ttccaacact acaaaatttt cttggccttt aactagaatt
     5221 caatgtcgtt tatttaaaaa aaaatacacg cctgtcatcc tgagcgtagc aaaggatctc
     5281 taatcgaccc cgtgagatga ttcgcttgcg ctcaacatga caaccctctc atgaaacgag
     5341 caacagcaag tatcacagag ttacacgatg taacacggtg ttacacagag cgtgtgacgt
     5401 gatattcacg aacaacgatt cacgaatagc gaagaacgct acccgccccg taggggcgta
     5461 atatttgtaa caccatgatt atacctaaaa tgtatagcga tgcacgcaat acattttcca
     5521 aagagtaaag gtgaagcgtt gcatcgcaac agaaaagcac tgcaaagagc gggttacatg
     5581 ttctttcttg tcttgataca agaaagaacc aaagaaaatc aaggctgaaa agtccgaccc
     5641 attgccgggt ctggctttta tgcctctgct tgcttcttcc gcttcattcc ctctcaggga
     5701 aaaggtctgg ggtgaggtcg tatcctacat tcaacctata tgacccctct aaatctcccc
     5761 tgagagggga gactttagcc ctgcaataag gtttccttat tgcttctccc ctctcctgtc
     5821 aggagagggg ctggggtgag gtcgttttcc acgaacaacc aacgacgctt cccgccccgt
     5881 gggggcgtaa tatttgtaac accatgatta ttcctacatg tatagcgatg cacgcaatac
     5941 attttccaat gagcaaaggt gaagcgttgc atcgcaacag aaaagcaccg caaagagcga
     6001 tttcccaaaa aacaacaagg gttaaaggta aagagacgct tcggctaact cagcatgaca
     6061 gcgtgatgtt tcgcttgcac tcaacatcac aaataaccat acctcaacaa catttaatta
     6121 agatttctgc tcatctatat gtttaacaaa attttatggt attcatgcag cattatcgtt
     6181 ttttcctctg tcttttgtat aaatcatttt aaaaatatac tgccatgaga ttaggaattt
     6241 tagctttgct gcttattgca gcaaccgtaa ccagcacaac tataagtgct caggaaaccc
     6301 gaaactttga tgctaaggga tttgacggaa tacgaaacag cacatcggct gaggtgcaaa
     6361 tttcacaatc gccaaactat ggtattgagg taaccgcaaa gagtgaggtt ttcaatgaac
     6421 tcgagatagt tgtaaaggag aatgttttgc atattaaaag caaggacaat ttaagatttt
     6481 caagtaactg gggtaaagta ttggtcaagg tttcatgtcc ggatattaaa cttttggctc
     6541 agaatggctc gggaaatatg attgcactaa cgcccataaa aggtgccgac gaatttacaa
     6601 cttcggtaaa tgggagtggc gacatattgc taaaagagat acaggccaat tcggtacagg
     6661 ttagcataaa cggttcggga aatattaaaa catcagcgca agggaaaacc tatacgctaa
     6721 aggccaactc taacgggtca gggaatatcg atcttgagac attaacctcg gagattgctc
     6781 aactaaagtt gaacgggtct ggcgatgcaa ccgtaaggtg caacaaggaa tttaaaggat
     6841 caataaacgg gagcggaagc atcacgcttt atggtaatcc tcttattgat gccaaggtta
     6901 acggttcagg acgattagtc cgtaagtaaa gattggataa cggtgcacaa aaaataacgg
     6961 ctgaggttat tcctcggccg tacatatttt aatatttggg atatgtgagg ttcacaattt
     7021 gatgaattgg tataggtttg ggatgcataa actaaattcc ccctcccaga atatgcaaaa
     7081 cttgcatata aatttgtagt tttgattttt attctaagct tttagtatca tgctcagagt
     7141 ggcagttgat tttgatggta caattgtgga gaatgaatat ccccagttgg gaaagcccat
     7201 gctatttgcc tttgaaaccc ttaaggaact tcaaaaaaga ggctttgtcc ttatcctttg
     7261 gactgtgcga accggtaagc tgctcgagga ggctgtggag ttctgccgcc agaatggggt
     7321 tgaattctac gcagttaatc gcaaccaccc tgaagagatt cttgacccgg agactcctcg
     7381 taagctcgat gttgatgttt ttattgacga tcgcaacctg ggtggatttc ccggttggga
     7441 taaggtgttc agcatgcttt gccccaacga gccctatgag gtttcgcaga aaaaggtttt
     7501 aaaacagtat aaaacgaaaa gttttgtacg acgtatcttt ggtatttaac atctatttta
     7561 tgctacaaag aatttcaatt ttgctattag ttctagccgt tgcttgcaca aatggccgaa
     7621 aggatcaact gcccgatacc actagtatgc cagcagagaa tcctttgcat cttatccggt
     7681 tcgacaagcc cacaagtggt tcattattca cagccggcga aagcattgat attacaatta
     7741 aactaaccga tactattcag cccgattcca ttgtgcttta ccgtaacagc gaacgaattc
     7801 agaaagttag caatttatcg ttcaccttac aaacgcaagc tggtaaaccc ggcacaattc
     7861 agctgaatgc tacagcatgg aaagatgggc gcaggcaaac agcatcaatt tccataaagc
     7921 tcaaatcgaa tacaaaacct gccagctaca gttaccgaat agtaaaagcc tatccgcacg
     7981 accccgaagc ctatacccag ggcttattct accataacgg ctacctgtac gagggtactg
     8041 gtcagcaagg cgcatcgagt ttaagaaagg ttgagctgga aactggaaaa atattacaga
     8101 gcgtaaacct aagcagggaa tactttggcg aaggcattgc cattcttgat gataaaattt
     8161 accaacttac ctggacaaac ggtacaggct ttgtttacga tgctgccact tttaaaccct
     8221 taaacacttt cagctatacc acgcagggct gggggctaac caccaatgga aacgaactta
     8281 taatgagcga tggctcaaat actatctact ttagagagcc aagtggtttt agtgagctta
     8341 ggagaattga agtatttgac aacaatgggc ccgttaaaat gcttaacgag ctagagtaca
     8401 ttgatggtaa aatttacgct aatgtttacc tgaccgatag gatagtaata attaaccccg
     8461 aaaccggagt ggtagaagga aatatcgaca tgcagggact tttaactact acacaaagaa
     8521 ccggcaagga agatgtatta aacggtatag catacgaccc caatggaaag cgaatttttg
     8581 ttaccggaaa gctatggggt aaactatttc aggttgaatt cataaagaaa aagtagatgt
     8641 caacccgcac tgaaaccgac taccttggaa gtttacaaat ccctgccgat gcactttacg
     8701 gaatacactc ggtaagggca aaagataatt ttcctgatac cacgcaattc catgttgagt
     8761 ggtaccaggc tatgggactg gtaaaacagg catgttacct tacctatgct gaaatggtta
     8821 aggcactcca ggaagaaaat ttattccgcg atagcaaagg ttttattccg cctaacgtag
     8881 taaatgccct aattgcagtt gcaggcgaag tgcatgaggg caagtacttc gacagtttca
     8941 ttgtcccggc catacaggga ggtgctggta caagtatcaa catgaatgtt aacgagataa
     9001 tcgctaacgc aacactcatc aggcttggcc ataatcccgg caactacacg gttatcgatc
     9061 ccattgagca agccaatgtt taccaaagca caaacgatac ggtaccaaca gcacttaggg
     9121 ttgccgtggt aaggttgtta accctgctcg aggagctaat caataacacc cgccaggcag
     9181 ttgaaaccat tgagaaaagt taccgaaact cattgcgtat aggatacacg cagctacagg
     9241 cagcggtacc atcgtcgtac ggacagctgt tcagcactta cagcgaagcg ctatcgcgcg
     9301 attggtggag ggtttcaaaa tgctttgagc gtataaaggt tgttaacctt ggcggaggag
     9361 ccattggtac agcagtggga acaccccgct actttgtaat ggaggtggtt aagcacctgc
     9421 aacagctaac cggattcccg tttacccgca gcgaaaacct taacgatgcc acagcaaacc
     9481 tcgattcttt tgttgaggtg catgccattc tcaaagccca tgcagtgaat attgagaaaa
     9541 tggcatcgga tatacgcctg ctggcgtcgg atattgcggg caatgcaatt aacattccca
     9601 aacgacaggt gggcagctca attatgcctg gcaaggtaaa ccctgtaatc cctgaatttg
     9661 tagtaagcat tgcccatcgg gtttacacca acgatatgct catctcaaac cttgccggac
     9721 agggtgcgct tgacctaaac gcctacctgc caaccattgg acacgccatg attgattcca
     9781 taaagctact gattgctgcc aaccaagccc tagcaggcca tttactaaat ggcattgaga
     9841 tcgactctga gaaatcggct cagctactga tgtttaatcc atccataacc acagccctgc
     9901 ttcctattat tggatttaac cgggctgcaa tgcttgcgca ggaaatgcgt ctgaaaaaaa
     9961 ttgatatttt cagcgccaac caaaacctga aactaattcc ggaagaaaag ctggttgaat
    10021 tggtaaaacc aaacaacctg cttaagctgg gattctcgtt tcgcgaggtg attgattaat
    10081 tctcaatatc tatttaagtt ttcacaaaac ataagcgacg gttgaaaaat ttgtttgtaa
    10141 atgataattc aattggagta atgaaaaaat ttactgaata catagttgtt gttgctgttg
    10201 ggttactttt tacctacctg tttgcaggcg atgtagttaa aaatataaat cacagatact
    10261 ttgcccaaag cggcgatggt gcaaaggact actatgccac attctaccac attaagcacg
    10321 ataaaagcta catacattcc aacagcatga attatccctt tggcgagagt atcttctttg
    10381 cgggttcgca gccaattgta agcaatacca ttaagttcat ttcacaaaac atatacgatg
    10441 ttagccctta cacagtagcc atatataacc ttctgattct gattagcatt gcctttacgg
    10501 ttcttgtaat ctacctgatt tttaaacatc ttaatctacc catttggtac tcgttacttg
    10561 tggctatagg gattactttc ctttcccctc aaattgacag gattggaggg cattttgcct
    10621 tgagctacac cattgtgctt ccgttattca ttctgctttt gctaaagttc gaccgccaaa
    10681 aaagtattgg tctatcagta ctcattcttt ttgctggaat gtgggcatca ataacccatg
    10741 gttacttttt cactttctac atggtgatgt ttttggccta ctggatctgg caaaaacctt
    10801 ggcgtaagga gtttgaatgg cgcaaacagt ggttacacgt ggtgctgcaa attgctattc
    10861 ctcttattgt tgtccctacc ctaattctgt acatagagcc cgttgaggat cgaacaacat
    10921 ggccatgggg ttttctgttt taccgtgctt ttcctgagtc cgtatttctt tcgccgggtc
    10981 gcccttactg gggctggctc aatagccttg taaaaatacg taacctccag tgggagggaa
    11041 ttgcctatgt aggattggtg tcaacaatca tatttctaat aggccttttc agctttatta
    11101 aaaatgctgt taataggcgt tggggaaaaa ccctgaccat aaccagtcag cctttaatgg
    11161 atcggctttt ctggctgtcg tttctactcc ttttattctc atttggaata ccgtttatct
    11221 ttaagctgga gttcctgctg gaataccttg ggcctattcg ccagttccgt ggtataggtc
    11281 gatttgcatg gcttttctac tacaccatga acattgtggc gttttaccta atttggcaga
    11341 aagttaaagg gtataaaccc atttacagga ttttactggt aatcccaata gccattttgc
    11401 tctacgatgc ctgttttcat agcaaatctc aaaaacactt tatcaacaat agcctacctg
    11461 agctggatta tgcctttcat tcaaacagta agcccaatat caatattgat gagtttcagg
    11521 ccatcatgcc aattcccttt tttagcgtgg gaagcgaaaa ctactggcac gaatcggcct
    11581 gctttggttc catggtaaag atgtatgccc tatcgcagct aacaggattg ccccttaacg
    11641 ccacctacac aagccgatca tcggtttggc gatgcgtgga aaacctcaac ctgattatgg
    11701 aaccgctcca ggagtacccc gtgcttaaat actacaacca tggcaaggat atccttttat
    11761 ttgttgaccc acattgcgac caaataaacc agaatgagcg ccgaattatt accatatcaa
    11821 ccccaattga tacactctgg gggtatgagc tacgaaggtt gaaagtggcc gaccttgcta
    11881 agctgcccga ggtataccgt aatgagtacc ttaacgctac ccctgcaatg gccgatacca
    11941 tttctggtaa ttactacttt caggaatttg aaagtactcc cacacccata gcgttcaaag
    12001 gaaacggggc gctcaagata aaccgacggg gttacacaaa cctttactgg gggcgcttgc
    12061 caaacaacag ctcatcggaa atcacggtgt cgttctgggt taaccgcatg aaagcagata
    12121 ttgtaccccg caatcgtatt gagattgtta ccggtaaaga ggaaggaaag tgggaaacct
    12181 ggaatgggtt tggttttatg gagaaaacaa aggcattctg gaacgattgg gcattggtgg
    12241 agtataacct gaaactcaac aacccgtccg attttatttg catttcggtt gctccattgc
    12301 tggctaaaag cggagatgtt tacattgata atgttcttat caaaccgtca aatttcaatg
    12361 taaaattcgt catgggagat tttgttttac tgaataacag gctgtttaac aatatttcaa
    12421 tcggttcaga ttagaatttt accctaacca tacacttcac ctcgctacgg gtattcccca
    12481 taataaggtc agggcctgac cctatagttg ccgaacctgg gaagtaggtt tgagcatatc
    12541 gtaaccataa atcaacaccc tttgagggtg agtatttaag catcgccata attctggttc
    12601 cattagagta gtaggctgga accgtaaagg agtaaagcaa atcgctctcg tacgagtaaa
    12661 ggcgggtatt ccatgattct gtatcgaaaa tggcaaagcg taacctgatg ctcaacggtg
    12721 ttttttgcag gttaatgttg atatcctgcg ctagcaggta tccatattca ccaccggaaa
    12781 ggctatcggt tctatagttc gatatctcca gggttgacct caggtttacc aaagggttag
    12841 gctgataggt aagctgagta cggaaacctt gtcgagtttg ggttagtgtt gatgttaccg
    12901 gaatattagc accagtcata tttttctcat cctgcttgta acggtaacga aaacttaact
    12961 gaaaatatgt atttagagag tgttccgaaa ggaaaagaaa atctctccct gccgatggcg
    13021 agtaaacccc atagcgcatc catgggaatt ggtaaaggtc aatatagccc gatattttcc
    13081 atccttttgc ggctagcaag cttaacccca tcagtaatcc cttttcattg gtagcacctg
    13141 agccctcagc aagggcagca gtgtaacggg ggttaaaggt tgtgctatag ctcctcccta
    13201 aaaccgaaag atgcactcgg ttacttagct tcaactgccc accggcaagg gctgccattt
    13261 tctgcgagaa aatatcgata gcaatctcac caaaaaacag atgctgacgg tagtagtaat
    13321 cggcatttat tcccatagca agtttctcgg tagaagcagg cgttaggctg taaatgggtt
    13381 gattgtttag gttttcacca taaattctgc tgtaaacagc agttgtgcca agccttatct
    13441 tattaagggt tagggttata ttactgccgg ttaccagttc tggaatattt cgtcggctcg
    13501 caatttccga agccgttcgg tggtaaccgg tttccggcaa cgatgaaaat gcaagctcac
    13561 ctgaactaac cgtatcgaca agattgccat ctattttttg gtatgaagct agcaggctaa
    13621 aatcgatatg cttgaagttt aaagtgaagc ctgcacccct caaaaaaagc gattcgtaag
    13681 ctgaggaatg tggcacaatg cccctgcccc gcttatgtag ccccattacg ttcgccgatt
    13741 ttccaaaggt taaactattc caaaatgtta gcccttgtcc aaattcagca cggaaatcac
    13801 caaccacaag gcgtttaact ttcccaacat ccgaaaccat tgcgtagcct gcaaggtaat
    13861 cgaaaccgga agggaaggtt ccgtcgagga gtggctcacc ggcatcgttc tcggctgtaa
    13921 ccccaatttg caaacggttt gccctgtaac ggtaacgggt gtaaattgca tacttgctcc
    13981 ctaagtaact gctcgcaccg ccataatcgc tcctatagcc aactggggtt tggagggttg
    14041 ttttgaggcg agtgaaaata tcgtgcctcc ccatagtaaa atcggaaact ttcaatggtt
    14101 ttacaacctc gccagccaca acaaatggag caagccgctc tacatcagtc aggtcgaatc
    14161 ccggaactaa ctgcaactcg taaacactaa gtattttgcc agtggaatcg cggtagctta
    14221 acaaactctc aatctgaaaa tcggtaagaa aaaatagctt ttgcaaatcg gacttacggg
    14281 cggcattcag gttcaaaggg ctctcggcaa gttgaagtaa atcctcaacc agctccgaat
    14341 aatcctgttc agtatcggag ttggcagcca tctcctcaag gatatcggct acaacctgtt
    14401 gcgggtcggt taccagctga gcattgctta ccaatgccac aaaaacaaaa agggatagca
    14461 aaaggtgttt cgccttcatt gggttttatt atcttttttg ccaaacaaat aggcaaccga
    14521 tagacttggt gtaaaaccaa gttgctgatg gtggcttaag ctgaagtcga tatcaaagcc
    14581 tttaacgggt aaccctactc cagcggtaat cccctcaggg cttgctgctg agtaacctaa
    14641 tcgcaaagcc gtacgttcca ttaccctata ctcaagtcca aaccgaaagg tgggcgcgcg
    14701 ttgggtgtcg tcgtcaattt gaaatgcaag taaaatattt tgtgagggca aataggttac
    14761 gcctaagccc attagggtgg gcaaatcacc taaatcgtcg aaggttgagc ccgtgggatt
    14821 aaaaacgtaa aagccaaagg ttagcttgga ggttggcata taatgaactc caccctcaac
    14881 agtaaaagcg gtggcattgc ggtaatcggc aggtagttgc atccgagtaa gggcaagtcc
    14941 taccccagca gcaaaacgtt tccccagaaa cattccatac cccaagccag ccctgcttgt
    15001 actaaacccg ctaaatccca aatgtgaaat atcaatacca aaagtaccag gttttacagg
    15061 gattgcaaat ccaagcatgc ttaggccaag ttctgaaagc ataaaccgat tctcatggtg
    15121 agctccaacc caaaactggc gctggtacgc taacgatgcc tgattggtaa aaccatccca
    15181 attgccttgc atggagagcg aacagtttcc catagcagcc gccgctccgg aaacaggccg
    15241 aatatcagct tgacaataaa ccgataggtt ttgtaatagt aaaagtagta ctatactaaa
    15301 ctgtcgaaat atttccataa atccagttta taccacaagt taaaaagaaa ttgaaaaaca
    15361 tgcaattgga tagtaaaaag ggatgtgtag cggaaaagct tattgatgtt gattgttgca
    15421 actgttcgaa aaaaggagtt aactttgcac tttattttaa tttgataaac catgaaacag
    15481 gtcaagctaa aagataagga gttccggtta agctatcccg agacagaaat tcagaacgat
    15541 atcgacttgg tagcatcaaa aatcaaccac gattttgcca agagtggcga agttccattt
    15601 tttgttagcg tgcttaatgg ttcctttatg ttcacagccg acctgttaaa acgtattaca
    15661 gtaccctgtg aggtatcgtt tgtaaaactt gcctcgtacc agggcacctc atcaaccggt
    15721 aaggtaaatg agctaatagg cttaaacgaa aatgtaaccg gcaggaccct tgttgttctg
    15781 gaggatattg ttgatactgg cattacttta agtaagctat acgaagaact ccgtaagctt
    15841 aacccaaaag agataagggt agccactctg ctctttaagc ctgaagccta taagggaaac
    15901 attaaactcg attacatagg ccgtagcata cccaacgatt ttatagtagg ctatggtctc
    15961 gattacgacg gacttggtag aaaccttcca gatatttaca ctctaattta gataatgaca
    16021 gcctaaacaa ttgcccacct gcgttttcag gtgggttttt gtttttggtt aaggattgat
    16081 atttatcaaa aatcggctaa ataaacaaac acccaaagca aaaaaaggta actttgaaat
    16141 ccgttgaaca gttcgtaaaa tatggattca ttagtcaata gccattttat tatacccttt
    16201 cttatatcca tgtttctggc catcaatatg ggaggtagtg gaacagggcc agcattttca
    16261 gttgcctttg gagcaaacgt actaaaaaag acctcggtac cagctctgtt tgggctaatg
    16321 gtatttttag gggcaataat tgcaggcaag gcaacctcgg caactgtggg taagggactt
    16381 gtaaaccctg agcttatgaa ctacgcccag gtgtcaatca ttctttttgc ggtttccata
    16441 tcgctacttg tggcaaacct ttttgggatt ccacaatcaa caagccaatc aacagtacta
    16501 gctgtgatag ctccaggcat ttacttccat gagcttaatt cccgcaaggt gttttttgaa
    16561 gttattccca catggtttat cctgcccatt attgcattca tcttaagtct aataattggc
    16621 aagtacattt acactccatt acggagacgg gggtatacta tatcgagccg gataaatgac
    16681 cattacatgc tgcgttgggc aataattttt gcctcgcttt acgtggcctt ttccattggt
    16741 gccaacaacg tggcaaatgc cagtggcccc ttaacctcaa tgaccatgaa tgagctgggc
    16801 ttggggaatc agggcggaag gctgcttatc cttatcatga ttatgtcaac catgatagtt
    16861 gccccgagct ttggcatagg cagttcgcta tttggtgaga aaatcctcag gaatacagga
    16921 aaagagctct tcctatttgg gcgaattgag gcagtaataa ttgctattat atctgcaacc
    16981 cttttgcttg cagcctcgct ctttaagggc atacccacat cgctggttca gctcaatgtt
    17041 ggggcaatac tgggtattgg cgttgcccgc cttggacctc gaaatatctt tagaaaaaca
    17101 gccgttcgta agtttttcct gatgtggctt attgcaccga ttattgcttt ccttatttcg
    17161 tggtacctta ccttcctggc cgataagtac ggataccttt aacttaagta cgatgagtta
    17221 ataaattcaa ccaggcttcg tacaaacaaa aaaagcgtac cctattggta cgctatatgt
    17281 tgttgttaac gagtattcta ctcaaacatc ccacgcattc tttcaaagaa tgagcctccc
    17341 tcatcggggt tgggggtaaa acttggcgat tttgcaaatt tctcaaaaat tgccttttcc
    17401 tcccgggtaa ggtttttggg tatgtaaaca ttaaccgcca caagtaaatc gccgcgctgg
    17461 tagctattca cctcgggcaa acccttaccc cttaagcgaa gaattttccc gggttgggtg
    17521 ccgggctcta ttttaatctt cactttacca tcaacggtag gaatttcagc cggtgcacca
    17581 agaactgcgt cgggaatgct gatgttaagg ttgtaaatta ggtcgttacc gtcgcgcacc
    17641 aggttagggt gtttttcctc ctcaatcatt acaagcaaat cgccagggac tcctccacgg
    17701 cgggcagcat tacctttagc ctgaacgtta agctgcatac cctcggcaac accagcaggt
    17761 attttaacgg ttattacttc gtcctcgcga accacacctt cgccattaca tgcaacgcac
    17821 ttgttggtta ttaccttacc ttcgccatta caggttgggc atggcgaagt ggtttgcatc
    17881 tgcccgagca gggtattagt tacacgggtt acataccctg aaccgcggca ggtagagcat
    17941 gtactatacg atgaacctcc tgccgcaccg gtacctccgc atgattggca cgatacgtac
    18001 ttgtttacct taatcttttt ctcaaccccg ttagcaatat cgcttaacga cagtttaacc
    18061 ttaacccgta gatcggtacc gcggtttgtg cgtcgcgatg agcgtccgcc accgaagcca
    18121 ccaaacccgc caaaactgcc aaaatggccg ccaaaaatat cgccgaactg ggcaaaaatg
    18181 tcctcaatgg tccagtcgtg cgagaaacca ccaccgcctg caccgttacc catacctgca
    18241 tggccaaact ggtcgtaacg ggcacgcttg ttatcgtcgc taagaacctc gtaggcttcc
    18301 gcagcctcct tgaacttctc ctcggcatcc ttatcgcccg gattcttatc ggggtggtat
    18361 ttgagtgcca ttttgcggta tgcctttttt atttcatcct tgctggcatt ccgcggcaca
    18421 cctagtattt cgtagtaatc gcgcttggtt gtcataccct acctatttta ctctccaacc
    18481 actacctttg agaagcgcat taccttttcg tgcaacatgt aacccttttg aattacgtca
    18541 actattttgc ccttctgggc atcgtcctgc accggaattt tggtaatggc ctcatgcaaa
    18601 tcagtgtcga gctctttgcc aatggcatca atctctttaa cgccttttgc cttaaggaaa
    18661 tcgttgaact tatttacaat taatgatatg ccttccttaa ccgcattaat atcgttagcc
    18721 gtttcaatgg ccttaagggc tctgtccagg tcatcgataa ccggaagcag gtccttgaga
    18781 acttcctccg acgcaaattt tatcaattca gctttctcct taagcgttcg tttacggtag
    18841 ttatcaaact cggcagtaag gcgcaaatac ttgtccttca tctcctccaa ctgggtagta
    18901 agctcagcaa ccttgtcatc attggcttga ctggcctcgg tttgctgagc atctccttga
    18961 ggagcttgtg tattactatc ggttacatta ttttcggtac tggttgtttg ctgattcacc
    19021 tgctcttgct caatatcctc ctttttatgt ttcttgctca tatatgatga tttttttacg
    19081 tttcacacta attcccaacc ttatgttcaa aatctattcc agctgttggt atcggtcaaa
    19141 ttgacatgga attatcatat attgatgtca ttaatacctt cttcaagcta agctgcatga
    19201 caaaattgtt atgcttcccc gtttagagaa aagcctaacc catataagct cattataacc
    19261 attccgaccc tttgcttgat tcggttcggc tactttgccc tcagcttaat atctttcacc
    19321 cgaagctgca gggtgatatt gcctttgtaa ttattttcat gtattgcaaa gcaagcgtca
    19381 aatggctggc catagtgtat tggtttatag tactcagcca tctgaaatgc aattgcatcg
    19441 aacttttggg tactaccttc ctgaaccagg gttaacttga ggtgctcctt ctcgctgcca
    19501 accagtcggg cttcgccgtt atcgtacaca ttttcagcca tgaaaactgg cgacatgttt
    19561 cctggcccga agggttgaaa ctgctttaac acgttgaaaa acctctctgt aatatcatcg
    19621 agcctaatgc gggtgtcaat atcaatttgt ggtgagagca tttcgggggt aatggtcttg
    19681 ctcacatgct cctcgaaccg ccttttaaac tcctcaaccc tatcaacctt catggtaagg
    19741 cctgcagcgt acatgtgacc accaaagttt tcgagtaagt ccgaacatgc ctcaatggcc
    19801 tggtacaggt catagccctg aaccgatcgt gcggagccgg ttgccaaatc gcccgattgg
    19861 gttagaataa tggtaggacg atagtaggtt tcaattaaac gtgaggctac aatgcccacc
    19921 acgcccttgt gccacgatgg gttaaaaagc actgtagagt agctgtgctg caaccgcttg
    19981 tcctgcgcaa tcatccttag cgcttcgtgg gtaatggtgc ggtcaacatg ctttcggtcg
    20041 ttgttacacg agtcaattag tgcaccaacc tcaaaggctg aactgtcgtc cgatgcgcaa
    20101 agcaggtcaa cagccgtttt accggactcc atgcggcctg ccgcattaat ccgagggcca
    20161 atgcggaaca ctatatcgtc aatagcaatc ctttggttct caatgccagc tattttgatt
    20221 atcgatttta gtcctcggct tggattggtg tttagctttt ccaacccata aaaagctaat
    20281 accctattct catccactat aggtacaata tcggaggcta tacttactgc aagtaaatcg
    20341 agatgttgat acagttcctc ctccgagatc ccctcctttt tgcagtaacc ctgtagcagc
    20401 ttaaatccta ccccacaacc cgaaagctcc ttaaacgggt acatgcaatc ggagcgtttg
    20461 ggatcgagca cggctactgc ttcgggaatt cgttcgccag gtaagtggtg gtcacagatg
    20521 ataaaatcga tattgcgaag cttagcatac ttaacctttt ccaccgcctt tattccacag
    20581 tccagggcaa taattagcga gtagccattc tcaaaggcaa aatcgatacc tttgaacgag
    20641 attccatatc cctcattgta cctatcggga atgtagtatc caatttttga gtacctcttg
    20701 cggaaaaaag agtacattag ggcaacggcg gtagtgccgt caacgtcgta atcgccgtaa
    20761 aacagtattg attcacccct acggatagcc tgctcaatac gctcaaccgc ctcggccata
    20821 tccttcatca ggaatgggtc gtacaggtcg gcaagcgatg gcctgaaaaa gcgtttagct
    20881 ttttcatagg tatcaacacc acgctgaacc agtagcgaag caatagtttc gttaacgttt
    20941 atttgcttag caagggaact taccaataca ggatcgcccg gatcctttag aacccatcgt
    21001 ttttccattt ctgcatgttt taaaagttaa acagtaccta cgcggttaca tgcgaaatga
    21061 aaccgatgag caaagtggtt aaggagttta gccttaacct cttccatatc aagctggcgg
    21121 ttaagttcca cctgcatgga ggttaccccc ttatcaataa acccgcaagg gtttatgtaa
    21181 cggaagtagt ccaagttggt gttaacgtta aaagctaagc cgtgcatggt tacatatcgg
    21241 cttgccctaa caccaatagc gcagattttg cgttcgcgac cctttacgcc agggtcgagc
    21301 caaacgccgg ttgctccttc gagcctatcg gctacaatac catactcctg aaggcaatcg
    21361 attactacct gctccagcag gtgaatgtaa gatttcagcg ttaatccaaa tgcctccagg
    21421 ttaaatatgg ggtaggcaac aatttgtccg gggccatgat aggttatatc gccccctcgg
    21481 ttgatgtggt agaacgatgc attaattctt ttaagcatct cttcggggat cagcaggttg
    21541 gcgttctcgc cacttttgcc aagggtataa acatgcggat gttcgcaaaa tagcaagtag
    21601 tgcaacttgc catcgccgtt cagcttacgt ttggttacct cgtcaaataa tttttcctga
    21661 taatcccatg cctccttgta atcaaccgtt ccaagatcat gaaactttac gttttgcatt
    21721 atatattgtt tttcctattt gcatagcaag tcaacccatt aaggttgatt acttacaaca
    21781 ccccttaacc aggtgttgtg ccttatcggc gtggtacgat gaacgcacca gtgggccgct
    21841 ttcggcaaat tcaaacccct tagctagggc aatctgctta tactcttcga actcctcggg
    21901 ggtaatatag cgttgaacgg gtaaatttgt ggggcgtggt tgcaagtact gtccaatggt
    21961 aaccattttg catcccacag caagcaaatc gtcaagggtt tgaattactt catcgcgggt
    22021 ttcacccaaa ccgagcatta tacctgattt tgccctaaaa ccgcgcttag caatatgctc
    22081 aagcactttc aagctccgcc tgtagcttgc ttttgttcta acaagcgggg taaggcgctc
    22141 cacggtttca aggttatgcc caaccacatc gggttccgat tccaggaaaa ggtcaataag
    22201 ttcaggcctc ccgtcaaaat cgggtattaa aacctctatg gtggtgttgg gattaaccct
    22261 tcgaatctcc ataacagtac tacgccaatg tccagctcca tggtcgggca aatcgtcgcg
    22321 gtcaaccgat gtaattacac agtaactaag acccattagg cttaccgtac gggcaacctt
    22381 agtgggttca ttgggatcgg gaggaagcgg ttttccagtt gcagtagcac aaaacttaca
    22441 cgaacgggtg catatatcgc ctagaatcat tagagttgca accccatttc cccagcactc
    22501 gcctatattc gggcacattc cactgctgca aattgtatgc aacccatgtt tcttaacaat
    22561 ggaattcacc tgggcatacc cttcgccacc gggtaacttt atctttagcc attccggctt
    22621 ccgttcattt cccatcccca aaaattttta caaaataact aaaaaatttg ctcttaaacg
    22681 attaatttgg agtaaaagta aatgtggtga gcagtgaacc gttaaagtta aaggataaaa
    22741 acacttccta tctttaacca tcaaccgtaa attaaaaaaa gtatcacagt gttacacaga
    22801 gtttacactg tgtttcacag tgtaaatcag cattatttgc gtaatctgcg ttctattttg
    22861 gtttaactac caacaatcaa ccaacaacca tcaacttaaa ccgtttcaca gtgcaaatcc
    22921 gctcaatctg cgtcatctgt gttccattac cttacacaaa caacaaaaat gtatttacta
    22981 ttctgccata cgaagcgaaa agtgatcctc gttgttcaag ttgctgtatt caattaaacc
    23041 cattgcggtt aggttaataa gtattttttc cgcttcggat actgagataa agcccaacct
    23101 gctgaaatgg tcaatggtga ttgtttgatg cgattttagg taatctatta atgcattttc
    23161 gggctctccg aatagtatgt taatgggttt acggtacttt tctgccttcc agatcttaat
    23221 ttgaacaggc gaagccacca cgttctcgtc gtttacacgt atatacgctt tataaactcc
    23281 cgttttatca ggtgctttat ggggcttgcg tcttgagggt tttaccttta cctccaacac
    23341 ggttttgccg tttatttccc acttaactac ttcaaaaggc acctcgggtt tacagtacag
    23401 ctgcgaggcc gcctcaacca tgtagtactc ctcatcggta ttcacaccgg ttatgttgcc
    23461 attatcctta acgccaatga gcaatgtacc tcccgcagta ttagcaaaag ctaccaacga
    23521 ccgtgcaatt ttgcgcgaat cggttataga gtgtttaaaa tcgagtgtaa gcccctcgcc
    23581 ctgcgatatc aaacctgcaa tataacggct cacaacttta ggttttatag gttaagatgg
    23641 caaaattaac tcttttagtg accccacctg taatgttcga tagcaaagcc aagcatctca
    23701 acaattctgt cggaaacggt atcaatcagt tcatccatgt ttgaaggcct actgtaaaac
    23761 gatgggcttg ctggcaaaac tacagccccg gcctgggtaa cggtaaccat gttttgcagg
    23821 tgtataagat tgtaagggag ttcgcgcggc accagtacta gctttcgtcg ttccttaagc
    23881 atcacatcgg ctgctcgaac tattaaatcg ttacttgtgc ctgaggctat tcgtcctaat
    23941 gttcccatag tacaaggaac tacaaccata gcattgtagc cggctgaacc cgatgccatg
    24001 ggggcgaaca tatcgttatt atcgtatagc tttacaggaa atccgaaagt gatatcgcga
    24061 tttagctcat attcccaaac ccttttcccg ttctcggaaa aaactacggc aacctcatcg
    24121 accctagccg ataattcaat tagcttttca ataaccctcg ccgcatatac agcaccactg
    24181 gcaccggtaa cagctacaat caccttttgc atctattaga ataattagtc taggatagaa
    24241 caaaggtaaa gaaaaaaggt tggaagttga cattattcaa atgtattcac cctttttaaa
    24301 gtagccattt tcggctttta ttattataca ttcatggtta catattttgc cttttcaaat
    24361 tggtgttcat ttactttacc attttgctta aagctaaaaa ctgagttaat ttaaataaaa
    24421 ctaagcgatt aggactaaac gtttttctac cggttaaatt gggtttaaat ttccactatc
    24481 tttgaaagta aattaggcaa taaacaatga caaaaaagaa gaataaaacc gatgccggtt
    24541 ccaaaccaaa tcgtatagcc aaaaaggagt taaagaaact cgttaccgat ttactgctgg
    24601 ccaatcctac caacatctat aactacaagc aggtatcgaa taaaattggg gttagtgacg
    24661 attatacccg tcgtatggtc aatgaaattc tctatgagct tgcgctcgat gggtttgctg
    24721 aggaacttca gcgtggcaag ttccgtgctg ttatgcagaa cgctctggct gttggcatac
    24781 tggagatgaa ccccgacggg tcagctgatg ttataaccga tgacaaccgt gagatatttg
    24841 ttcccgactt taggctgaat cacgcactgc atggcgataa ggttcaaata tcaatttacc
    24901 gccaacgccg taggggttta cttgagggtg aggtggtgcg cgtgattgag cgtgccaaac
    24961 gcaactttgt tggaaccatc aactttgtag gtaaaaacgc atttgttgta cctgataaca
    25021 agcttatgcc ctacgatatc ttcatcccaa aggttgagct gaaggatgtc cgaaacgggc
    25081 aaaaggtgat tgttcagatt accgattggc cggcaaggga aaagaatccc aatggaaaaa
    25141 ttgtagaggt tcttggcgat cctggagttc acgaggtgga gatgcacgcc attctggccg
    25201 agtatgagct accataccgt ttccccgaag agctggacat attagccgaa aagattagcg
    25261 acaagataac cgaagccgat taccgtgagc gacgcgattt ccgaaacgta cccactttta
    25321 ctatcgatcc ctttgatgcc aaggattttg acgatgccct atcggtgcaa accctaccca
    25381 acggcaactt tgaggtgggg gttcacattg ccgatgttac ccactacgtt aagcctaata
    25441 ccccaatcga caacgaggcc attgagcgcg gcacatcggt ttacctggtg gataggtgtg
    25501 tgcccatgct tcccgaaagg ctatcgaact atatctgctc attacgtcct aacgaggaaa
    25561 agctttgctt ttcggctgtg tttgagctga acgagaatgc cgaggtgctt aaccagtggt
    25621 ttggccgtac ggtaatcctg tcgaagcgtc gattcaatta cgaggaggcc caacaggtta
    25681 ttgaaaccgg caagggcgat atgagtgaac aaatcctgct gctccacaag ttggctcaaa
    25741 aactccgggc agagcgattc aaaaaggggg ccatcgcatt tgaacgcgtt gaggtaaagt
    25801 tcgatcttga taccaaggga aaaccccttg gggtttactt caaggagaat aaggaatcga
    25861 atcaactcat tgaggagttc atgctgcttg caaaccgcag ggtggctgaa tttattggaa
    25921 tgcgaaagga tgggcgcaat gagctgcctt ttgtttaccg cattcacgac aaacccaacg
    25981 aggagaaact gaatgctttc cgttccttta tcacccgttt tggttacagc atttccggta
    26041 ctaccgataa gcaggttagc aaatcgctaa accagcttat ggaaaaggtg aaggggcgcc
    26101 cagagcaaaa tcttattgaa accctggcct tacgctccat ggccaaagcg cgttactcta
    26161 ccgataacat tggccactac ggattggcct ttaggtatta cacccacttc acatcgccaa
    26221 tacgccgcta tcccgatatg atggtgcatc gcctgttggc acactaccta aacaatggta
    26281 agccaaagga tagggagcaa attgaaatgc tttgtaagca ctccaccaac atggagatca
    26341 aagcaactga ggctgaaagg gcttcaatta agtacaagca ggtggagttc atgcaggata
    26401 agctgggcga aacctttaca ggtgttatat cgggcgtaac caattttgga atattcgttg
    26461 agctaaccga ttctcagtgc gagggattgg tttccattcg cgatttagac gacgattact
    26521 accgtttcga tgaggaggag tacgccctgg ttggtcaacg tacaggccgc aagttcacct
    26581 taggcgatga ggtggaggtg gaggtttggc gcactaacct tgccaaaaaa cagctcgatt
    26641 ttaagctggt gggcatgaag ggtagaagca gtaaaccttt gaaacctcgt aggagtcaaa
    26701 cgaaaagtag tcctaaaaat ccaaaagtgg catcaagcag aaagggacaa caaaaggcca
    26761 agaaaaagga gaaacgtcgt aaacgctaaa acatcaaaat agatgaaacg atttatcgtt
    26821 attgcactgg cttgggcagc atttgcctgc tcaaccaaaa atgagttaac acctggctca
    26881 tggcttggag taatccaaat ggactcaatt cctggcaggc tggatgttcc ctttaacttt
    26941 gaggttaagg ttgataatga caaggttcag cttaccgttc gtaatgccga tgaacttatt
    27001 gaaataaccg aggttgaacg cattggcgat tcgctttacg ccaagttccc aacctttacc
    27061 agcgagctgg taatggccgt aaacgattcg ctgggcggct actactatcc caaaggggta
    27121 aaggatggcc gcaggtataa gttttacgga attaaagggg aaactgaccg tttcccatgg
    27181 tttaccgaac aacctaagtt cgatattacc ggacgttggt ggtttattga gaatccgggt
    27241 acgcctgact caaccgttat ggtcgccgaa ctcaaacagg aaggttcaag ggttactgga
    27301 accatactca gcactaccgg cgattaccgc taccttgagg gtaaggttgc tgggaacatg
    27361 ttctacttct caaagtgcga tggcgctcaa tccattatag taagaggcaa aattaccgat
    27421 tctgccacca tggtaaacgg tctcatatcg ggtagcccac gctgggcttc ggcctggcgc
    27481 gccttacgcg acgataaagc ccaactcccc gaggcccaaa gcctggtttg ggtgcgcaag
    27541 ggttacacaa cctttgagtt tgctggaacc gacctaaacg gcagtcgtgt aacctccaac
    27601 gatgaacgct tcaaaggcaa agtgttagca gttcttgccg gcggaagctg gtgcccaaac
    27661 tgccttgacg aaggccgttt ctacaaaacc atgtacgaaa agtaccgcga taagggcttt
    27721 gaggtggttt cgctctgctt tgaggataaa acctttgagg ctgcccaacc caagattaaa
    27781 cgttttgccc agagcattgg agccagctac accttcctat acgttgcccc ccgtggacgc
    27841 gaacagcgcg actcagtact ctatgccctt gaggggcaaa tggcctaccc cacaagcatg
    27901 atattggacc gcaacggcaa aatacgccgg gttgaaaccg gattctcagg ccccggtacc
    27961 ggtgagcatt accagaaatt tgcccacgaa accgaggaac tcatagttaa actacttgaa
    28021 gaaaaatagt tatgaactga aaaggtggtg ggtaaaaccc catacaacta acccatttta
    28081 aacactggaa tatgaatgat ttacttgcat ttggtttact aggatttacc tcatttttta
    28141 cgttgattaa tccattgggc gccatgccca tatttatgac aatgactgca gagctgaatc
    28201 ctgccgagag aataaaaact gcccgaaaag cttcaattgt agcttttctt acaatactct
    28261 tttttgcatt tactggtcaa ttactcttca ggttttttgg aatttcagta aatagtttac
    28321 gggttgttgg tggagtaata ttttttatga tgggaatgga catgctccag gcacgattaa
    28381 cccgagtaaa gatagccgaa aacgaggtga aaaagtacgt aaacgatatt tcaattacac
    28441 cgctggccat tcccatgata tgcggccctg gagcaatcac taatgctatt attctaatgc
    28501 aagatgctac aacaattgat aagaagatgg tattagtttt tagcattttt ctggtgatgc
    28561 ttttaacctt tttaatactc tatagttcct caaagattat aaaagcgttg ggcgaaacag
    28621 gtaataatgt tctaatgagg cttatgggtt taattgtaat ggttattgct gttgaattct
    28681 tttttgctgg catcaaacca gtacttatcg aaatatttgg gaaataaagg cttacaacca
    28741 atttattgcc aagcaacaac tggaactctt aactcgtacg tgtcttgaga agcaagtgaa
    28801 cg
//
LOCUS       DAOOWJ010000023        28730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1645_length_28730_cov_8.723976, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000023 DAOOWJ010000000
VERSION     DAOOWJ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 28730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28730
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1645_length_28730_cov_8.723976"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1271)
                     /locus_tag="PLW22_03910"
     CDS             complement(117..1271)
                     /locus_tag="PLW22_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOU73883.1"
                     /translation="MSEKFQMVAKTLQGFEEVLAEELKEIGAENIEVGCRSVSYYGTK
                     ETLYKSNFWLRTALRVLKPIYVFNARTIDEFYGNARKFDWTSVMELSHKFAVESVVNS
                     ELFPHSRYIALKLKDAIADQFRDRFGKRPFVDPKKPHITFHIHVNNDVCTVSLDSSGE
                     SLHRRGYRVAQDVAPINEVLAAGLIKLSGWNGDSVFIDPMCGSGTILIEAALIAYGIA
                     PGIFRQHFGFENWLDFDEQLLQSIYNDDSRERDFEYLILGRDISKQAIASAMENIKES
                     GLNKKIDLGVASIFDYNPPKVDNGIVITNPPYGERLKKEQIENFYKQLSDVFKNKYAG
                     YDVWIISSNQEALRSFGLRPSKKINLLNGALECKYQRFSMYKGSLKSKQS"
     gene            complement(1286..2863)
                     /locus_tag="PLW22_03915"
     CDS             complement(1286..2863)
                     /locus_tag="PLW22_03915"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU73884.1"
                     /translation="MEINNEFRQLGLSVKALLAIKKKGFEQPSPIQKITIPVLLNESC
                     DIIAQAQTGTGKTAAFGLPIIDLLDGQPKGIKALVLVPTRELALQVCDEMLSLADKRI
                     IITAVYGGQSISEQQRRIKKGVDVVVGTPGRILDLINRGDLDFSQLQIFVLDEADEML
                     NMGFIEDIEEIMRHTPKEKRVLLFSATMPDRIVKLAKNYMKEPKRLEVDKAKPTTGLT
                     DQIYFEVREGDKFDALTRIIDIEPEFYGLVFCRTRNDVDVVTGKLIERGYAAEGLHGE
                     ISQAQREKTLSRFRKKHINILIATDVAARGIDIVDLTHVINYSLPQDPESYIHRIGRT
                     GRAGKQGTAITFVSPEEYRKLNYFQKATKVNIRKELLPTAEDIVAVKREKIKEELVEI
                     INQGNYMDYLGMADDLIETNSPEVALAALLKLAFKSQLDVSSYTEIRTFRVDTKGTTR
                     LFIGLGKKDGMTPKKISEFIRKEVKIPDRNIDDILVRDDFSFITVPFTDAEKILSAFS
                     NRTQDGKPLVTKAKPKK"
     gene            complement(2935..4527)
                     /locus_tag="PLW22_03920"
     CDS             complement(2935..4527)
                     /locus_tag="PLW22_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU73885.1"
                     /translation="MKKLLSIFLLVFALFFSAGAQNNSSFSAYKFVRFIQYLSGNYVD
                     TINVNQLVDDAIIEILGQLDPHSIYISKEDVQAMNEPLEGNFEGIGVEFNILNDTLIV
                     VNPISGGPSERVGIMAGDRIVEIDGKPITNIGLKIPDVHKLLRGPKGTRVDVKILRKG
                     VSQLLDFTIIRDKIPIYSIDASYMARPGIGYIKINRFAATTEQEFVEALEKLEKEKMA
                     DLIIDLRNNGGGFLNSAYELASYLLEPGKLVVYTQGRNSPRTDYFSHFRGRKPFKGRL
                     VIITDEGSASASEILAGAIQDWDRGIVVGRRTFGKGLVQNQLPLPDGSMIRLTVAKYF
                     TPTGRAIQKPYKMGDVKDYYSDLDNRYSRGEFFNQDSVHFPDSLKYTTLVKKKVVYGG
                     GGIMPDVFVPLDTTYYSEYYGKLVRSGLINQFAITWVDANRELLKKKYPKFEVYNKNF
                     EVTDNILDELYSYAESRQIARDSTGIQTSKAELKLQLKGLFAQSLFGSGFYYQVTNEH
                     NESFIKSLEVINNWTRYKHLVQ"
     gene            complement(4544..6073)
                     /gene="guaA"
                     /locus_tag="PLW22_03925"
     CDS             complement(4544..6073)
                     /gene="guaA"
                     /locus_tag="PLW22_03925"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOU73886.1"
                     /translation="MHQKILILDFGSQYTQLIARRIREFNVYCEIHPFNRIPKLDNTV
                     KGVILSGSPFSVRDNNAPMPDLTDIRGRYPLLGICYGAQLLSHTNGGEVLPSAIREYG
                     RATLEVVDSNCPLFTSLSPKSQVWMSHGDTIAKIPDNFKVVASTADVKVGAFSVLGEQ
                     TYGIQFHPEVYHSVEGSKLLKNFVVDICGCNQDWTPASFIDETVSNLRNQLGNDKVVL
                     GLSGGVDSSVAAMLLHKAVGPNLTCIFVDNGLLRKNEFSQVLESYKTLGLNVIGVDAS
                     QRFYSELKGVSDPEQKRKIIGRIFIEVFDEEAHKIKDVKWLGQGTIYPDVIESVSVNG
                     PSATIKSHHNVGGLPEKMNLKVIEPLRLLFKDEVRRVGKELGLPGGILNRHPFPGPGL
                     GIRILGEVTPEKVRLLQEADSIYIQGLREAGLYDKIWQAGAILLPVQSVGVMGDERTY
                     EFVVALRAVTSTDGMTADWFHFPHEFLARISNEIINRVKGINRVVYDISSKPPATIEW
                     E"
     gene            6333..7817
                     /locus_tag="PLW22_03930"
     CDS             6333..7817
                     /locus_tag="PLW22_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOU73887.1"
                     /translation="MRIRVVKTNSNAKAVQVVSYQNNKRVILKHIGSAHSDDTLKELM
                     ILANEWVKDYTGQLSIFSDSNPNSVLHTNHSSFIGVKYHLFYRQIRAIQRQIGFDGLP
                     ALLNDLVAIRIFEPASKLRSLALLEQYFGIIHSRKNFYKIAPECVNLKGIAEEKVVHF
                     AREHYSFDYDLLFYDVTTLYFETFEEDELRRNGFSKDNKFQQPQILVALMVTKEGFPI
                     AYEVFPGNTFEGHTIVPVIKDFIARNRVKNLTVIADAAMISTANIKQLTQNNINYIVG
                     ARLGNVADTMVETIDKKICREDGKSIRIKTSNGYLVCSYSSVRYRKDKYEMEKQIEKA
                     KEAVTRPSKNKKLKFIKTKDEHAELNEALIEKTKKLLGIKGYYTNLEEKVVSNKIIIE
                     RYHELYRIEQTFRISKSDLQTRPIYHFKEQPIRLHILVCFMALVVSKHIEIRTGTSIN
                     RFIQEAKKNADGEIINHITQKIITIKAHPTQKMNELIEKLFSPH"
     gene            complement(7905..8834)
                     /locus_tag="PLW22_03935"
     CDS             complement(7905..8834)
                     /locus_tag="PLW22_03935"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOU73888.1"
                     /translation="MTFSVTILGSSSALPTSKRFPSAHVLNVHERLFLIDCGEGTQIQ
                     LRRFKLPMNRINHIFLTHSHGDHVLGIFGLISTFNLLGRKYDLHVYGVADFEEIFYKN
                     IDYFTDQMSFKVIYHVVDPFKVETIYSDKHVVVTTLPLRHRVPTVGYLFREAPRPLNI
                     RKELIAKYNLSLTDIVKIKNGSDYTLNDGTVIPNAELTYISSLPRSYAYCSDTLYSER
                     LVGLVKNVDLLYHEATFLHSDLRLARLTGHSTAQQAATVALKASVGKLLIGHFSSRYK
                     NEKDLQREAMEIFPATEVVNDGLTFSVEPKSTY"
     gene            complement(8831..9193)
                     /locus_tag="PLW22_03940"
     CDS             complement(8831..9193)
                     /locus_tag="PLW22_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU73889.1"
                     /translation="MEFKIQKFEKFTQIEVLEEKLDTNIAPSLKSELVLISGNGERNI
                     VLDLSSCRYCDSSGLSAILVANRLCKNAGGTFVLTGLTDAVERLITISQLDTVLNIAY
                     SKDEAVQIIEKAENEAKK"
     gene            complement(9235..11064)
                     /gene="rpsA"
                     /locus_tag="PLW22_03945"
     CDS             complement(9235..11064)
                     /gene="rpsA"
                     /locus_tag="PLW22_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOU73890.1"
                     /translation="MNDEMAKEALKNQNAGIPDFDWTSYADDHAYDQNQRKELEKVYD
                     QTLSTVAENEVVEGAVVAITKREVFVNIGYKSEGVISINEFRYNPDLKVGDKVEVYVE
                     SSEDKKGQLVLSHKKARVMRSWERVNEAFDKNEIIKGYIKCRTKGGMIVDVFGIEAFL
                     PGSQIDVKPIRDYDVFVGKVMEFKVVKINHEYKNVVVSHKALIEEELEQQKKDIIAKL
                     EKGQVLEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRVNHPEEVVQLDQKLNVVILD
                     FDDDKKRIALGLKQLTPHPWDSLDPNLKVGDKVKGKVVVIADYGAFVEIAPGVEGLIH
                     VSEMSWSQHLRSAQEFLKVGDEVEAVILTLDREERKMSLGIKQLKPDPWTSIEEKYPV
                     NSKHKAKVRNFTTFGVFVELEEGVDGLIHISDLSWTKKIKHPAEFTNIGDEIEVVVLE
                     IDKENRRLSLGHKQLEENPWDVFETIFTVDSVHEGTIVEIFDKGAVIALPYGVEGFAT
                     PKHLVKEDGSTAKVDEKLAFKVLEFNKSAKRIIVSHSRTFEDEKKVEEKAEKKSKASS
                     AQKVVKKLNSNIEKTTLGDISELQALKSELEANQKKSDSKSEE"
     gene            11257..12195
                     /locus_tag="PLW22_03950"
     CDS             11257..12195
                     /locus_tag="PLW22_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU73891.1"
                     /translation="MRKRVLVTGGAGFIGSHLCERLLSEGNEVICLDNYFSGNKQNIV
                     HLLANPYFELVRHDVTVPYYAEVDEIYNLACPASPIYYQQNPIKTIKTSVMGAINMLG
                     LAKRVKAKILQASTSEVYGDPEIHPQPEHYWGNVNPIGPRACYDEGKRCAETLFVNYH
                     MQNGVKIKIARIFNTYGPRMSLSDGRVISNFTVQALTNQNITIYGDGSQTRSFMYIDD
                     LVDALIMLMNTPESVTGPINIGNPEEYTIIDLAKKIVALTGSKSEIIYKAATVDDPKQ
                     RKPDMEVARKILPKWTPKVNIDDGLTKTIEYFKQQL"
     gene            12271..13149
                     /gene="rfbA"
                     /locus_tag="PLW22_03955"
     CDS             12271..13149
                     /gene="rfbA"
                     /locus_tag="PLW22_03955"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOU73892.1"
                     /translation="MKGIILAGGAGTRLYPITKSISKQIIPIYDKPMIYYPLSVLMLA
                     QIKEILIISTPKDIHLYQDLFGDGSQLGLKIQYAIQPSPDGLAQAFIIGEDFIGTDSV
                     CLVLGDNIFYGGNLSELLKESAKLNDGAIVFGYYVKDPHRYGVAEFDETGKVLSIEEK
                     PENPKSNFAVTGLYFYSNDVVEKAKSLKPSKRGELEITDLNRLYLNEGRLNLKIMGRG
                     MAWLDTGTHESLLQASNFIQTIEDRQGLKVACLEEIAYRNGFISKEQLLKLAEALANN
                     QYGQYLYRIANEKVYL"
     gene            13161..13727
                     /gene="rfbC"
                     /locus_tag="PLW22_03960"
     CDS             13161..13727
                     /gene="rfbC"
                     /locus_tag="PLW22_03960"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOU73893.1"
                     /translation="MTVEPTQIPDVLIIKPKVFTDHRGYFFESYNVDAFKKMGIEINF
                     VQDNESYSRKNVIRGLHYQLSPYSQTKLVRVVAGRVFDVAVDIRKNSPTFGQYVGVEL
                     SDENKYQLLIPKGFAHGFAVLSNFAIVSYKCDQYYNPEADRGIRYNDPEIGIDWKIPM
                     EQAIVSAKDNVHPAMKDAELNFVFGSTY"
     gene            13733..14590
                     /gene="rfbD"
                     /locus_tag="PLW22_03965"
     CDS             13733..14590
                     /gene="rfbD"
                     /locus_tag="PLW22_03965"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOU73894.1"
                     /translation="MKILVTGANGQLGTAIKKESSTFPDLTIFYTDIEETDICSKESV
                     QLALKKYAPNVVVNCAAYTAVDKAEDEEDKAFALNAHAPANLAEQCKLMGVKLIHIST
                     DYVFDGKSNVPYVETDRVNPQSVYGKSKLEGEQRSMALGNTMVIRTSWLYSSYGNNFM
                     KTMLKYGKERPELRVVFDQTGTPTLANDLAYAILRIAESPERLFIPEIFHFSNQGVCS
                     WYDFAYEIIKQAGLTPKITPITTSDYPTKAIRPQYSVLNKQKITNLLEMEIPHWRQSL
                     VKCLQEYLR"
     gene            14612..16345
                     /locus_tag="PLW22_03970"
     CDS             14612..16345
                     /locus_tag="PLW22_03970"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOU73895.1"
                     /translation="MVEKEIMEKAKQWLNPPFDEETRARVAQMIEQDPKELTESFYCD
                     LEFGTGGLRGIMGVGTNRMNIYTVGMATQGLCNYLKNNFKHLPEIRAAIAHDSRNNST
                     LFANVAAQILSANGIKVYLFDALRPTPELSFAIRTFKCQCGIVVTASHNPKEYNGYKV
                     YWEDGGQIVPPHDKGIISEVKAISSIEQVNFKGNDNLIVKVGRELDEIYLNTLSTLSL
                     SPDIIEKYRDMPIVYTPIHGTGVNLVPMALRKFGFKNIITVDEQCVVDGNFPTVASPN
                     PEERATMQMAIDKAIKTNAEIAMATDPDADRVGIGIRKPNGEFTLLNGNQAASILIYY
                     ILKKWHEHNRITGNEFVVKTIVTTDLISSIAHGFGVDCYEVLTGFKYIAEKIREFEGV
                     KKFIAGGEESYGYLYGDFVRDKDAVISCAMFAEIAAWAKSQSKTLYDILSDIYREYGL
                     YKEHLISLTRKGIDGLAEIKAMMDAFRSKPPQTIGGSKVTSIIDYQNQVFVDVHTGKE
                     KHTNQPKSDVMQFITADGTKVSVRPSGTEPKIKFYIGVKGTMAANDDIDTALAKLDKK
                     IETIVAELNLR"
     gene            16348..16764
                     /locus_tag="PLW22_03975"
     CDS             16348..16764
                     /locus_tag="PLW22_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOU73896.1"
                     /translation="MKSYRKELWFETRNRREYINITRDVEVCLAESGIKEGLCLVNAM
                     HITSSVFINDDESGLHHDFEVWLEKLAPEKPYSQYKHNGFEDNADAHLKRTIMGREVV
                     VAVTNGKLDFGPWEQIFYGEFDGKRRKRVLVKIIGE"
     gene            complement(16804..18585)
                     /locus_tag="PLW22_03980"
     CDS             complement(16804..18585)
                     /locus_tag="PLW22_03980"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOU73897.1"
                     /translation="MTLTIQERLSLLRKQMKKADVDAYIVHHSDPHLSEYIPDYWKER
                     EWLSGFTGSAGTLVVTQKNAALWTDSRYFIQAEMQLSGTGIELCKIGMPETPDIQSWL
                     ALNLKQNSIVACNGLLISLSNQRTLSENLKAKKFKVRFDTDLVSKIWDDRPPIPQQKV
                     FVHTAEFAKVSVQEKLGRIRKELLVRNATAYLMCALDEICWTFNIRGADISYNPVFLA
                     YAIVDDDKATLYVDENKLTDEVIQFLANEQVSVKPYSKIFDKLAKLKKKDVLVLDPAK
                     VNFSIYSAIPGNTKVIETTGLATQIKARKQPVEVEGINDAMVQDGIAMVEFLYWLEQN
                     VKSGKVIEITVAEKLLEFRSKRKNFISESFGSIVGYADHGAIVHYSATPQTSYTIKPE
                     GFLLVDSGGQYLNGTTDITRTIHLGNPTPQEKRDFTLVLQGMVRLSMAKFPKGTRGSQ
                     LDTYARMALWSQGLNYGHGTGHGVGYFLNVHEGPQQIRPENHLPIEPGMVMSNEPGLY
                     RPNAYGIRIENLIVCVEDETNDFGSFLRFDTLTLCPIDIKAIDLDMLTVQEREWLNHY
                     HQLVYFKLSPHLAAEHEKWLKEITKPL"
     gene            complement(18640..21123)
                     /locus_tag="PLW22_03985"
     CDS             complement(18640..21123)
                     /locus_tag="PLW22_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU73898.1"
                     /translation="MLQNHNNNLGLGYPFNVRHFLSNLNRYYKAGFWFWDSNTNRIHF
                     GKKFVNALGFKESDNDNIPLDEFFKLVHRDDKKSLEKSFQRLRHNSALHQEMDFRLKI
                     REEWQHKRIFAFAVKDNPDASSYYIIGQFRNVDPVKSDTNTHDESITFERFANLLHKH
                     QLALLEIDLDGTVVNWNKSTTRLFQFDSSNTQSVSLREGFADDTWKKFQEWLLSGSGK
                     PFTSIYRNISGNEFNLSWSKLADIPQDKKRFIVAARDISQLVEFQNQIKRHDFQNEVI
                     SSFFSQIKGQTTPDEIFLLLGQTIEKAFPKSISIVFSYSADDSFVTLESIFGVKTKAW
                     DTFIDELGWNPVGRRLFIEKEKLATLISANVQENIIPFNELLDGIVSATTFKVFERTF
                     KIGKNYISGIVKDDKLYGGIILFQTSDSEPIDTQFLNRISIVASTVIDMSKANLDSCQ
                     MADGLKKQLAEKYELLTYINHSIRTPLNSIIGFSSMLDFTELDKSLQTEYLRIIQNQS
                     KSLLDLTNELQDFVRISTGALTVIKTKQNVNDFFHELKANIDFRLGLLELYNIKVNYT
                     IPENTDFLELYIDSGRLNQALSIYFIQFIKFIQTGSLNIGYSIEDDKIKIFIHEGDSS
                     IDSKIRTLFTDDLKALLNGTYSHSSHFNILLANRLLTLLEGEVEILNDGKLKLEVKFG
                     LIANEAQPQADELNEEQAIKRVEFKNSVVLIVEDEEVNYLILNELLTAWGVSTIWAKN
                     GREAVDLVSSLNQGINLILMDIRMPVMDGYAATMEIKQVNSAIPVVAQTAFSAPQERL
                     KAQAAGCNGYITKPIDPNILHHVLEMYLA"
     gene            21361..21564
                     /locus_tag="PLW22_03990"
     CDS             21361..21564
                     /locus_tag="PLW22_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73899.1"
                     /translation="MNSRRRGFNTEKLKRVHRKEILFNTSELEAINHYCKRYKVRNKS
                     KFLREAIISKILNKFDQDYPRLF"
     gene            complement(21573..24029)
                     /locus_tag="PLW22_03995"
     CDS             complement(21573..24029)
                     /locus_tag="PLW22_03995"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU73900.1"
                     /translation="MSVKPPYYIFQCVLVIFITGFASLHAQERRVKISGIVKDTLNMP
                     IPSASVIIVGSSLGVSTDDEGRFSITVTDIDTISIRFSCLGYQTQEMNVKAERGKFLE
                     VILKPSEEHIEGVYVRSQQRKVGNIERIGIDGLKTSPNVSGGLESLVKLLPGVSSSNE
                     LSSQYSVRGGNFDENLVYVNDVEVYRPFLIRSAQQEGLSFINPDMVGAVEFSSGGFTA
                     EFGDKMSSVLNVRYREPKANALRVSAGLLGASATLEGVAIGSSLTYMSGVRYKTSRYI
                     LNSLETKGDYTPSFFDWQGIADYKLSERITIGLLGNVAINRFDFQPRVRETRFGVFNN
                     TLQLKIYYDGQEKDRYLSSMAALIAAIRVTPNSNVKVIGSSYYSNERETYDLLGQYFL
                     NELDNSMGSKTYGDSLINVGIGGFLDHARNHINLKVQSLNLLGDMWFGKSLIKLGVQL
                     QREVLSDELLEWGLVDSSGYAIPYTGSQVGLDRFFGAASTIESYRALGFVQADLHFNV
                     GNWQYRTNIGFRVSHWSFNKEILFSPRLALSFNHSNYRSLQYHLSFGYYYQPPFYREI
                     RLASGELNRNIKAQQSIHALAGAEYYFTAWSRPFRMSLEFYNKWLNRLIPYSVDNVRL
                     RYSGENLARGYARGADLKINGELVSGAESWLSLSILKTKEDNLYDSYTDEGGNTVYPG
                     YYFRPTDQTFSVSLFLQDYLPGDKSFRVNLTGVYGSGLPMLDSSVPDQKSFRMPSYKR
                     IDVGFTKSFIDNGKRLVGNLKIANLSVGVDIFNLFNFNNTVSYLWVQTVANQEGNSYR
                     FAVPNYLTSRRVNVRFQMNF"
     gene            24151..24576
                     /locus_tag="PLW22_04000"
     CDS             24151..24576
                     /locus_tag="PLW22_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73901.1"
                     /translation="MKEILIVSQNNACRSAMAEGWFKYYARGAANVTSAGIEPTQLDL
                     NAAQSMMDAVIDITKHQPKSLNSLTDKEFDIVLVLDNKVAKTASSNLQYKNLYVFHFD
                     SPKAAKSDDSLTRKAYDNLRDDIENWAFDFIHQHIKRLM"
     gene            24578..24985
                     /locus_tag="PLW22_04005"
     CDS             24578..24985
                     /locus_tag="PLW22_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOU73902.1"
                     /translation="MIKQIEIVLRPYPQGFHLVTAEIVNQLPELPLVGLLHLFIKHTS
                     AALSINENADPSVRTDLNAFFNRHYPMEPYFKHDLEGDDDMPAHIKTTVVGTSISIPI
                     TNGKLNLGTWQGIYLCEFRKHGGSRKIIATVIE"
     gene            25259..26875
                     /locus_tag="PLW22_04010"
     CDS             25259..26875
                     /locus_tag="PLW22_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU73903.1"
                     /translation="MNNNKLLLLGDEAIAQGAIDGGLSGIYAYPGTPSTEIMEYVQAS
                     KQAAELNIHRQWSANEKTAMEAAIGMSYAGKRAMVCFKHVGLNVAADAFINASITGVN
                     GGLIVVSADDPSMHSSQNEQDSRFYGKFSMIPILEPSNQQEAYDMAYFGFELSERLNV
                     PVLMRITTRMAHSRAGVQRRESRPQNEIRLPSDPRQFVLLPSIARKNYKKLLVSQTAM
                     ELESEKSAYNQMIEGTDKSLGIITTGIAYNYLIENFPDHKCPYPVLKICQYPIPRKQV
                     TELYNSCSKLLILEEGYPLVEEMIKGFLNIGKTIHGRLDGTLPRDGELNPNIVAKALG
                     LTVTEGEPVPEVVKNRPPSLCQGCGHRDVYEDLNEALKEYGTGRVFSDIGCYTLGALP
                     PFNAINSCVDMGASITMAKGAADAGLVPAVAVIGDSTFTHSGMTGLLDAVVENSPITV
                     IISDNSTTGMTGGQHSSATGRIVEICKGIGVHPDHIRVVTPLKKNHEENVKIIKEELA
                     YNGVSVIIPQRECIQTFARRKKAEKKETNE"
     gene            26894..27475
                     /locus_tag="PLW22_04015"
     CDS             26894..27475
                     /locus_tag="PLW22_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOU73904.1"
                     /translation="MKNDIILAGVGGQGILSIAAAIGLAAVNSNLYIKQSEVHGMSQR
                     GGDVQSHLRISDKPIASDLIPAGKADMIISVEPMEGLRYLPFLAQTGWLVTNVTPFNN
                     IPDYPNINDILSKIKAVKNHIAIDADAIAKEAGSTKASNIVMLGAASLFLDIPFEKIE
                     EGIRQLFGRKGDEVVQLNLKALRLGREFSEKNK"
     gene            27566..>28730
                     /locus_tag="PLW22_04020"
     CDS             27566..>28730
                     /locus_tag="PLW22_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOU73905.1"
                     /translation="MENLGFHLPISEPITVFGITLLVIFIVPLVFSKLKLPGIIGLII
                     AGMILGTSGLNILDKERSIELLGNVGLIYLMFLAGLELEVGSFLKNQRKNITFGLFTF
                     TIPFLFGFTICKYFLGFGYLPSLLIASMFSTQTMISFPIVSRLRLTKTLPVEISIGGT
                     IITDTLVLLIFVIITSLSANSGLNGFLLLIPKILAFVLFIILLLPRFTHWFLRNLSSD
                     QMAQYVFVLATVFTTSAIARFLGIEPIIGAFLAGIVLNRQIPPSAMLMNRIEFIGNAL
                     FIPIFLFYVGMLIDLKAFVSGYESLELSAILISFAIISKWLAAYLTQKIFKLKTLDRQ
                     LIFGLSTAHAAATIAIILVGYKIGIVDINVLNATIVVIFASCVVSAMVTERAAR"
BASE COUNT         8589 a         6192 c         5760 g         8189 t
ORIGIN      
        1 tgattcaagc aacacctatg taatttatat ttaaacaaat ataataaaat tttaattgat
       61 aaatgcaaga aagtacgcac gatttttaaa caaaaaaagc cgtatttaaa tacggcttag
      121 ctctgctttg attttaaact tcccttatac attgaaaatc gctggtactt acactccaaa
      181 gctccattga gtaagttgat tttttttgag ggacgaagtc caaaacttct gagcgcttcc
      241 tggtttgagc ttataatcca aacatcgtac cctgcatact tattcttaaa aacatcggag
      301 agctgcttat agaaattttc aatctgctcc ttctttagcc tttcgccgta aggcgggttg
      361 gttattacaa tgccattatc tacttttgga ggattgtaat cgaaaattga ggccaccccc
      421 aaatcaatct ttttattcaa ccccgactcc ttgatatttt ccatggctga ggcaatagct
      481 tgctttgata tatcgcggcc tagtatcagg tactcaaaat cacgttccct actatcatcg
      541 ttgtaaatgg attgcagtag ttgctcatca aaatcgagcc agttctcaaa cccaaagtgt
      601 tggcggaaaa tacccggagc aataccatag gcaattagtg ctgcttcaat cagaatggtg
      661 cctgaaccac acatggggtc aataaacacg gaatcgccat tccaccccga tagcttgata
      721 agccctgcag caagcacctc gttaattgga gctacatcct gtgctaccct ataccctcgc
      781 ctgtgtaacg actcgcccga tgaatcgagc gatacggtac aaacatcgtt attcacatgg
      841 atatgaaagg tgatgtgagg ttttttgggg tcgacaaaag gacgcttgcc aaacctatcg
      901 cggaactggt cggcaattgc atctttcagt tttaaagcaa tgtaccgtga atgtgggaat
      961 aactccgagt tcacaacgct ctccacggca aatttgtggg aaagctccat tactgaagtc
     1021 caatcgaact tgcgggcatt cccatagaac tcatcgattg tacgggcgtt gaaaacataa
     1081 atgggtttta gaactcgcag agcggttctt agccaaaaat ttgatttata gagagtctcc
     1141 ttggttccgt aataggaaac cgaacggcaa ccaacctcaa tgttttcagc tccaatttcc
     1201 tttagctctt cggccaatac ttcttcaaaa ccctgaaggg ttttggctac catttgaaat
     1261 ttctcgctca tgattactaa gctatctact tcttgggttt tgcttttgtt accaaaggtt
     1321 taccatcctg ggttctgttt gagaatgctg agagaatttt ctcggcatca gtaaacggta
     1381 ctgtgatgaa cgaaaagtca tcgcgaacca gtatatcgtc gatattccta tcgggtattt
     1441 tcacctcttt acgaataaac tctgaaatct tcttgggagt cataccatcc tttttgccaa
     1501 gtccaatgaa caagcgtgtt gttcccttgg tatcgacacg gaaggttcta atttcagtgt
     1561 aactcgatac atcgagctgg cttttgaagg caagctttaa cagggctgcc agggctacct
     1621 ctggactgtt ggtctcaata agatcatcag ccattccaag gtaatccata tagttgccct
     1681 ggtttataat ctcaactaac tcctctttaa tcttttcgcg cttaacagca acaatatcct
     1741 cagccgttgg aagcaactct ttacgaatat taacctttgt ggctttctgg aaatagttca
     1801 gcttacgata ttcctcgggc gaaacaaatg taatggctgt tccctgcttt ccggcacgtc
     1861 cggtacgtcc tatgcggtga atgtacgatt cgggatcctg tggaaggctg tagttaatca
     1921 catgggttaa atcaacaatg tcgatacccc gtgcagccac atcggtagca ataagaatat
     1981 ttatatgctt tttacggaaa cggctcaagg ttttttcacg ctgtgcctgt gaaatttcac
     2041 catgtaatcc ctcagcagca taaccccgtt caattagctt tccggtaact acatccacat
     2101 cgttgcgggt acgacagaac accagtccgt aaaattcggg ctcaatatca ataattcggg
     2161 taagagcatc aaacttatcg ccttcgcgca cctcaaagta aatctgatcc gttaagccag
     2221 tggtgggttt tgccttgtca acctcaagcc tcttgggctc cttcatgtag tttttagcta
     2281 gcttcacaat acgatcgggc attgtagccg agaaaagtag tacccttttc tccttgggag
     2341 tgtgacgcat aatctcctca atatcctcaa tgaaacccat gttaagcatt tcatcggcct
     2401 catcgagaac aaatatctgg agctgactaa agtcgagatc gccacggttg attaggtcga
     2461 gaatgcgacc gggtgtacca accaccacat ctacaccttt ttttatacgg cgttgttgct
     2521 ccgagattga ttgccccccg taaacagcgg taataataat tcgcttatcg gccagcgata
     2581 gcatctcgtc gcaaacctga agtgcgagct cgcgggttgg tacaaggact aatgccttaa
     2641 ttcccttggg ttgaccatcg agtaaatcga ttattggtaa gccaaaggct gctgttttac
     2701 cagtacctgt ttgagcttgg gctattatat cgcagctttc gttcagcaat accggaatgg
     2761 ttattttctg gataggcgat ggttgctcaa aacccttttt cttaattgct agaagggctt
     2821 ttaccgataa acccaactgc ctgaattcat tatttatctc cataacataa ttaattggtc
     2881 aaaaaacgac ctgtgaaaag tattaaaaca ctaatttaaa aaaaaactga aaaattattg
     2941 tactaaatgc ttatatcgag tccaattgtt aatcacctca aggcttttaa tgaacgattc
     3001 gttgtgttca ttggtaacct gatagtaaaa acctgaacca aaaagcgatt gggcaaacaa
     3061 acctttcagc tggagtttta gttcagcttt ggaagtttga ataccagtgc tatcgcgagc
     3121 tatttgccgg gattcggcat aggaatagag ttcatccaat atgttatcgg taacctcaaa
     3181 gtttttgttg tagacttcaa acttcgggta tttcttctta agcaattcac ggttagcatc
     3241 aacccaggtt atagcaaact ggttaattaa acctgatcga acaagtttac cgtagtattc
     3301 agagtagtaa gttgtatcga gtggaacaaa aacatcgggc attatgccac cgccaccata
     3361 aaccaccttc tttttaacca gagtggtgta cttaagcgaa tcgggaaaat ggacactatc
     3421 ctgattgaaa aactcacctc tactataccg attatccaaa tcggaatagt aatcctttac
     3481 atcacccatt ttgtatggtt tctggatggc tcggccggta ggtgtaaagt atttggccac
     3541 ggttaaccta atcatggaac catcgggcaa gggaagttga ttctggacaa gccctttgcc
     3601 aaaagttctt cgaccaacaa ctataccacg atcccagtcc tgaattgcac cagcaagaat
     3661 ctcgcttgcg gaggctgaac cctcgtcggt tattatcaca agccggccct taaatggctt
     3721 acggccacgg aaatgggaga agtagtcggt tcgggggcta tttcggccct gggtataaac
     3781 cactagctta ccgggttcaa gaaggtagct tgccagctcg tatgccgagt taaggaagcc
     3841 tcctccattg ttccttaaat cgattattaa gtcggccatt ttctccttct caagtttctc
     3901 aagagcctca acaaactcct gctcggttgt ggcagcaaat cgatttattt tgatataccc
     3961 aatacccgga cgagccatgt atgaagcatc aatactgtaa atgggtattt tatcgcggat
     4021 tatggtaaaa tctagtagct gggaaacacc cttacgtaaa attttcacat caaccctggt
     4081 acctttgggc ccacgcagta gcttatggac atcggggatt tttaacccaa tattggtaat
     4141 tggcttcccg tcgatttcaa ctatcctgtc accggccata ataccaacac gttccgatgg
     4201 acctccggaa atgggattaa ctactattaa ggtatcgtta aggatgttaa actcaacacc
     4261 aattccctca aagtttccct caaggggttc gttcatggcc tgaacatctt cctttgagat
     4321 atagattgaa tggggatcga gctgtcccag aatttcaata atggcatcgt caaccagctg
     4381 atttacgttg atggtatcaa cgtagttacc tgataggtac tgaataaagc gaacaaattt
     4441 atatgcgcta aagctagaat tgttttgagc gcctgccgag aaaaacaggg caaaaactaa
     4501 aagaaaaatt gacaataatt ttttcataca cagtaataaa attttactcc cactctattg
     4561 tggcgggggg ttttgagctg atatcgtaaa ccactctatt aatgcctttt accctattaa
     4621 taatctcgtt tgagatacgg gccaggaatt cgtgagggaa atggaaccaa tcagcggtca
     4681 ttccatcagt agaggtaaca gcacgtaagg ctactacaaa ttcatatgtg cgttcatcgc
     4741 ccattacacc cactgactgg acgggaagta gaatggctcc ggcctgccaa atcttatcgt
     4801 acaaacctgc ttcgcgtaat ccttgtatgt aaatgctatc ggcctcctgc aagagtcgta
     4861 ctttttctgg tgtaacttcg ccaagtattc taatccccag accaggcccg ggaaaaggat
     4921 ggcgattaag gatacccccg ggtaaaccca actccttgcc aaccctgcga acctcatcct
     4981 taaaaagtag gcgtaaaggc tcaattacct ttaggttcat tttttcgggt agcccgccca
     5041 cgttatggtg cgattttatg gtggctgatg ggccattaac cgataccgat tcaattacat
     5101 cggggtagat agttccctga ccaagccatt ttacatcctt aatcttatgc gcttcctcgt
     5161 caaaaacctc aatgaagatt cgcccaataa ttttccgttt ctgttccggg tcggaaacac
     5221 cctttagctc actatagaat cgttgactgg cgtcaacgcc aattacattg agacccaaag
     5281 ttttgtagga ctccaatacc tgcgaaaact cgtttttacg aagtagtcca ttatcaacaa
     5341 atatacacgt caaatttggg cctacagctt tatgcaacag catagcggca accgatgagt
     5401 ccaccccacc agaaaggccc agaacaacct tatcattacc taactggttt ctgagattcg
     5461 atactgtttc atcgataaac gatgcgggtg tccaatcctg gttgcagcca caaatatcaa
     5521 ctacaaaatt tttgagtagc tttgatccct caacgctgtg atacacctcc gggtgaaact
     5581 gaataccgta ggtttgctcg cccaatacgc taaatgcgcc tacttttaca tcggcagtgc
     5641 tggcaacaac tttaaagtta tcaggaattt ttgctatggt atcgccatgc gacatccaaa
     5701 cctgcgattt tggcgatagg ctcgtgaaaa gcgggcagtt actatcaaca acctccaaag
     5761 tagcccggcc gtactccctt atggccgatg gtaaaacctc acccccattg gtatgggata
     5821 gtaactgtgc tccgtagcaa attccaagca aagggtatct acccctaata tcagtcaaat
     5881 cgggcattgg agcattatta tccctcacgg aaaaagggct tccggatagt ataaccccct
     5941 taacggtatt gtcgagcttt gggattcggt taaatgggtg aatctcgcag taaacattga
     6001 actctctaat tctacgtgct attagctggg tatactggga gccaaaatcg agtatcaaaa
     6061 ttttttgatg catggtaaca caaacacttt gaattgcaaa ggtaagaaaa tagaacaaat
     6121 tattggccga tttgcttatc ggttttaata tttatgatta aatagctcaa ataattgatt
     6181 aataggttga ttttggttca accgaaaagg tgaggccgtc gtttacacct gacttgtacc
     6241 cataaaattt agtgcggttt taaatatttg aatatcagat agtatgtata gaaaaagtgg
     6301 taattttacc gcactaaggc actaatttaa aaatgagaat acgggttgta aaaacaaatt
     6361 ctaatgcaaa agctgttcaa gtggttagct atcaaaataa taaaagagtt attcttaagc
     6421 atattggctc tgcccattcc gatgatacgc taaaggagct aatgatactg gccaatgagt
     6481 gggtaaagga ttacacaggt cagctttcta ttttttccga ttcaaatccc aatagtgttt
     6541 tacataccaa tcacagctca tttataggcg taaaatatca tttgttttac aggcagataa
     6601 gggctatcca gagacaaatc gggtttgacg gtttaccagc gttgctgaat gatttggtag
     6661 caataaggat attcgaaccc gcttctaaac tccgctccct tgctttactt gaacaatact
     6721 ttggtatcat acatagccgt aagaactttt acaaaatagc ccccgaatgc gttaatctaa
     6781 aggggatagc tgaagaaaag gtggttcatt ttgccagaga gcattactcc tttgattatg
     6841 acctgctttt ttatgacgtt accactcttt actttgagac ctttgaggag gatgagttgc
     6901 gcaggaatgg cttttcaaag gacaataaat tccaacaacc acaaatacta gtagccctga
     6961 tggtaaccaa agaaggtttc cctattgcat atgaagtctt cccgggaaac acatttgagg
     7021 gacacaccat tgtccctgtt attaaagatt ttatcgccag gaatagggta aaaaacctca
     7081 ctgtcattgc agatgctgcg atgattagca cggcaaacat aaagcaatta acccaaaaca
     7141 atatcaacta catcgttggc gctaggcttg gaaatgtagc cgatacaatg gtagagacca
     7201 ttgacaagaa aatctgtcgt gaagatggca aaagcattcg aattaagacc agtaatgggt
     7261 atctggtatg tagctattca tcggtcagat accgtaagga taagtatgaa atggaaaaac
     7321 aaatcgagaa agccaaagaa gcagttacaa ggccatcaaa gaacaaaaaa ctcaagttta
     7381 ttaaaaccaa agacgagcat gcggagctta acgaagcatt gattgaaaaa acgaaaaaat
     7441 tactgggaat aaagggatac tacaccaacc tagaggaaaa ggttgtaagc aataaaatca
     7501 taatagaacg gtatcacgaa ctttatagaa tagagcaaac ttttagaatt tcaaagagtg
     7561 atttacaaac ccgacccatt taccatttta aggaacaacc cataaggctg cacattctag
     7621 tatgttttat ggccttagtt gtatcaaagc atattgaaat cagaacaggt acgtctatta
     7681 atagatttat tcaagaagca aaaaagaatg cagatggtga aataatcaat catatcactc
     7741 aaaaaatcat aactattaaa gctcatccta cccaaaaaat gaatgaactt atcgaaaaat
     7801 tattttcacc gcactaaaag ggacaagtca ggaaaataga acaaattatt ggccgatttg
     7861 cttatcggtt ttaatattta tgattaaata gctcaaataa ttgattaata ggttgatttt
     7921 ggttcaaccg aaaaggtgag gccgtcgttt acaacttctg ttgctgggaa tatttccatt
     7981 gcttcccttt gcaaatcctt ttcattcttg taccgcgatg agaagtgccc aattagcaat
     8041 ttccctacgc ttgcctttaa ggcaaccgtt gctgcctgct gtgcggtact atgaccggtt
     8101 aaccgagcta atcgtaaatc cgagtgtaaa aaggtggctt cgtggtaaag taggtcaacg
     8161 tttttcacta gtccaacaag cctttcgctg taaagcgtat cggagcagta agcataggac
     8221 cggggtagcg atgaaatata ggtcaactca gcatttggaa taacagttcc atcgtttaaa
     8281 gtatagtccg aaccattttt tattttaaca atgtcagtaa gcgaaaggtt gtactttgct
     8341 attagctcct tacgtatatt caagggtcgt ggggcctcgc ggaacaaata ccccaccgtt
     8401 ggaaccctat gccgcaaggg caaggttgtt acaactacat gcttatcgga ataaattgtt
     8461 tcaaccttaa aaggatcaac aacgtggtaa atgaccttaa atgacatttg gtcagtaaag
     8521 tagtcgatat tcttatagaa tatctcctca aaatcggcta cgccgtaaac atgtaaatcg
     8581 tacttgcgcc ctaaaagatt gaaagttgat attaacccga aaatgcccag cacatggtcg
     8641 ccatgtgaat gggtaagaaa gatatggttg atgcggttca ttgggagctt aaacctgcga
     8701 agttgtattt gggttccctc gccgcaatca attaaaaaca accgctcatg tacattgagc
     8761 acatgagcgg atgggaaacg tttagatgta ggtaatgcag aactactacc cagtatggta
     8821 actgaaaacg tcacttttta gcttcgtttt cggctttctc aattatttgc actgcctcat
     8881 cctttgaata ggcaatatta agcactgtat cgagttgcga aatggtaatt aaacgttcaa
     8941 cggcatcggt aagtccggta agcacaaaag ttccaccggc atttttacag agcctgtttg
     9001 caaccagaat agcacttaac cctgaagaat cgcagtatcg acaactgctc agatcgagta
     9061 caatgttacg ctcaccattg cctgagatta aaaccaactc agattttaaa ctgggtgcaa
     9121 tattggtatc aagtttttct tcaagcacct caatctgggt gaacttctcg aatttctgaa
     9181 ttttgaattc catttctttc aggattaaat ggttaaactt tcgcaactca attattactc
     9241 ttcgctcttg gaatcagatt ttttctgatt tgcctcgagt tccgacttca gagcttgcag
     9301 ctcagaaata tcccctaagg tagtcttttc tatgttagaa ttcaactttt ttacaacttt
     9361 ttgtgcggaa gaggccttag attttttttc tgctttctct tcaaccttct tctcatcctc
     9421 aaaagtacgg ctatgcgaaa cgattatgcg tttagcgctc ttgttgaatt ccagcacctt
     9481 aaaggctaat ttctcgtcaa ccttagcagt tgaaccatct tcctttacca aatgtttagg
     9541 agtagcaaaa ccttcaacac cgtatggtaa ggcaattaca gcgcctttgt cgaaaatttc
     9601 aactattgta ccttcatgaa cggagtcaac ggtgaaaatg gtctcaaaaa catcccatgg
     9661 gttttcctca agctgcttgt gaccaagact taagcgacgg ttttccttgt cgatttcaag
     9721 aacaaccacc tcaatttcat cgccaatgtt ggtgaattca gcaggatgct taatcttttt
     9781 ggtccagcta aggtcagaaa tgtggattaa gccatcaaca ccttcctcta actcaacaaa
     9841 cactccaaaa gtagtgaaat tgcgaacttt tgccttgtgc ttggagttaa cagggtactt
     9901 ctcttcaata cttgtccagg gatctggttt gagctgttta ataccaagcg acatcttgcg
     9961 ctcttcacga tcaagagtta ggataacagc ttcaacctca tcgcctactt tcaggaactc
    10021 ctgagcactg cgtaggtgct gactccacga catttcagaa acgtgaatta ggccctcaac
    10081 gccaggggca atctcaacga atgcaccata gtcggcaata acaacaacct tacctttaac
    10141 cttgtcgcca accttaaggt ttggatcgag cgaatcccaa ggatgagggg tgagctgctt
    10201 gagaccaagt gcaatacgtt tcttatcgtc gtcaaaatcg agaattacca cgttaagctt
    10261 ctggtcaagc tgcacaacct cttcggggtg gttaacacgg ccccaggaaa ggtcagtgat
    10321 gtgaataagt ccgtctacgc cgccaaggtc gatgaataca ccataactgg taatattttt
    10381 aacggttcct tcaagaacct gacccttttc gagcttagca ataatatctt tcttttgctg
    10441 ttcgagttct tcctcgataa gcgctttatg cgatacaaca acgttcttgt actcgtggtt
    10501 gatttttaca actttgaact ccattacctt tcctacaaat acgtcgtagt cgcggatagg
    10561 tttaacatca atttgagaac cgggaaggaa tgcctcaata ccgaatacat ccacaatcat
    10621 accgccctta gtacggcact tgatgtaacc cttaataatt tcatttttgt caaaggcctc
    10681 attaacacgt tcccatgaac gcattacacg agcttttttg tgcgaaagca caagctgacc
    10741 ttttttatcc tcggagcttt ctacgtaaac ttcaaccttg tcgccaacct tcaaatcggg
    10801 gttgtaacgg aattcgttga tgcttattac gccttcggat ttgtagccga tgtttacaaa
    10861 aacttcacgt tttgtaatag caactacagc accttcaacc acctcattct cggctacggt
    10921 tgatagggtt tgatcgtaaa ctttttcaag ttctttgcgc tggttttgat cgtaggcatg
    10981 atcatctgcg taagatgtcc aatcaaaatc aggtattcca gcattttgat tctttaaagc
    11041 ctctttggcc atttcgtcat tcattgtcat taactttcag attgttaatt tgttagacat
    11101 tatttgtaga taaaattcaa cttgcaaaaa tacataactt tctaaaataa aatcaaattt
    11161 acccaattat tttgaaatgt atatcaacaa gtttggttga tttgtatcaa atatggccga
    11221 aattacctac ttttggtaaa gtaaatgcta ttgaacatgc gaaaacgagt attggttaca
    11281 gggggagcag gctttattgg ttcccacctt tgtgaacggt tgctttccga gggtaacgaa
    11341 gtaatttgcc tcgacaacta cttcagcgga aacaagcaaa atattgtgca tttgctggct
    11401 aacccctact ttgagctggt taggcacgat gttaccgttc cctactatgc cgaggttgat
    11461 gaaatttaca acctagcctg ccctgcctcg ccaatatact accagcaaaa ccccattaaa
    11521 accattaaga catcggtaat gggtgcaatt aacatgctgg gactggcaaa aagggtaaaa
    11581 gccaaaatat tacaggcatc aaccagcgag gtttacggcg atcctgagat tcatcctcaa
    11641 cctgagcatt actggggtaa tgtaaacccc attgggccaa gggcctgtta cgatgagggc
    11701 aaacgctgcg ccgaaaccct gtttgtaaat tatcatatgc aaaatggcgt taaaatcaag
    11761 attgctcgta ttttcaacac ctacggccca aggatgagtt tatccgacgg cagagttata
    11821 tccaatttta ccgttcaggc cctaaccaac caaaacataa ccatttacgg cgatggctct
    11881 caaacccgta gctttatgta cattgatgat ttggttgatg ctctgattat gttgatgaac
    11941 acccctgaaa gtgttaccgg cccaataaac ataggtaacc cagaggaata tacaataatt
    12001 gacctggcta aaaagatagt tgcattaaca ggaagcaaat cggaaataat ctataaagct
    12061 gccactgttg acgacccaaa acagcgaaag cccgatatgg aagttgcccg aaaaatcctt
    12121 ccaaagtgga caccaaaggt gaatattgat gatggattaa caaaaaccat tgaatatttc
    12181 aagcaacagc tgtaattaag attttgtaaa actgatgcca aagcctattt ttgtgtaatt
    12241 ttttaaaagt tgattagtaa aaaaaacacc atgaaaggaa taattttagc ggggggagct
    12301 ggaacacggt tatacccaat aaccaaaagc atttcaaagc aaatcattcc catttacgat
    12361 aaaccaatga tttactaccc cttatcggtg ctaatgctgg cacagattaa ggagatactc
    12421 attatttcaa caccaaaaga tatacaccta taccaagatc tttttggcga tggttcacaa
    12481 ttgggtttaa aaattcagta tgccattcaa ccatcgcccg atggtttggc tcaagccttt
    12541 attattggtg aggactttat tggtaccgat agcgtttgcc ttgtattggg cgataatatt
    12601 ttttacggcg gaaatcttag cgagctgctt aaggaaagcg ccaagctgaa cgatggcgct
    12661 atagtttttg gctactacgt taaagatccg catcggtatg gtgttgctga gtttgatgaa
    12721 acagggaaag tattaagcat agaggaaaag cccgaaaatc ccaaatcgaa ctttgctgtt
    12781 acggggcttt acttctacag caacgatgtg gtagaaaagg caaaatcgct taaaccttcc
    12841 aaaaggggtg aactagaaat taccgatctg aacagactct acctaaatga gggtaggcta
    12901 aaccttaaaa taatgggacg aggtatggcc tggctcgaca ccggaaccca tgaaagcttg
    12961 cttcaggcat caaacttcat tcaaaccatt gaggacagac agggtcttaa ggttgcttgc
    13021 ctcgaagaaa ttgcttaccg aaatggattt atctcaaagg aacaactgct aaaactagcc
    13081 gaagccctgg caaacaacca gtacgggcaa tacctttaca gaattgcaaa cgaaaaagtt
    13141 tacctttaaa atatttcagt atgactgtcg agccaaccca aattcccgat gtgctgatta
    13201 ttaaacctaa ggtttttact gaccacaggg gttatttttt cgaaagttac aatgtagatg
    13261 ccttcaaaaa aatggggata gaaatcaatt ttgttcagga taacgaatcg tactcaagaa
    13321 aaaatgtaat ccgtggtctg cattatcagc tttcgcctta ctcacaaacc aaactggtta
    13381 gggttgttgc cggtagagta tttgatgtgg cggttgatat cagaaagaat tcccccacat
    13441 ttgggcaata tgttggtgtt gaactctccg atgaaaataa atatcaactg ttgattccaa
    13501 agggttttgc acacggattt gccgttctaa gcaattttgc tatcgttagc tacaagtgcg
    13561 atcaatacta taaccctgag gctgataggg gtattcggta caacgatcct gaaattggga
    13621 ttgattggaa aattcctatg gagcaggcca ttgtatcggc taaggataat gttcacccgg
    13681 caatgaagga tgccgagcta aactttgttt ttggttcaac ctactaacta ccatgaaaat
    13741 tcttgtaacc ggagcaaacg gtcagctagg aactgccatt aaaaaggaat caagtacttt
    13801 tccagattta actatttttt ataccgatat agaagaaacc gatatctgta gcaaggaatc
    13861 ggttcagctg gcacttaaaa agtatgcacc taatgttgtt gtgaactgtg cagcctatac
    13921 ggcagtggac aaggctgaag atgaagaaga caaggcattt gccctgaatg cccatgctcc
    13981 cgcaaacctc gccgaacaat gcaaattaat gggtgttaag ctgatacata tttcaaccga
    14041 ctatgttttt gatggtaagt ccaatgtccc ctacgttgaa accgataggg ttaacccaca
    14101 atcggtttac ggaaaaagta agcttgaggg ggaacaaagg tctatggcac ttggcaatac
    14161 aatggtaatc agaacatcgt ggttatactc gtcttacggt aacaatttca tgaaaaccat
    14221 gctaaagtac gggaaggaac gccctgagct tagagtggtt tttgaccaaa caggaacccc
    14281 tacccttgcc aacgatcttg catatgcaat tcttcgaatc gctgagtcac cagagaggtt
    14341 attcataccg gaaatcttcc atttttccaa ccagggagtt tgcagttggt acgattttgc
    14401 ttacgaaatc atcaaacagg ctgggcttac cccaaaaata acgcctatta caaccagtga
    14461 ctaccccact aaagcaattc gacctcagta tagcgtgttg aataagcaaa aaattactaa
    14521 cttgctggaa atggaaatcc cacactggag gcaaagctta gtgaaatgct tacaggaata
    14581 cttaaggtaa aacactaaaa aatactacaa tatggttgaa aaggaaataa tggaaaaagc
    14641 caagcaatgg cttaatccac cttttgatga ggaaaccagg gcaagggttg cccagatgat
    14701 tgagcaagac cccaaggagc taaccgaatc gttttactgc gatttggagt ttggtacagg
    14761 cggtttacgt ggaattatgg gtgttggcac aaatagaatg aacatatata ctgttggcat
    14821 ggccacccag ggcttatgta actacctgaa aaacaacttt aaacatctac ctgaaatacg
    14881 ggccgccatt gcacacgact cccggaacaa cagcacccta ttcgccaatg tggcagcaca
    14941 gattctaagt gccaatggca taaaggttta cctgttcgac gcccttagac caacaccaga
    15001 acttagcttc gcaattagaa cgttcaagtg tcagtgtggt attgttgtaa ctgcatcgca
    15061 caatccaaag gaatacaacg gctataaggt ttactgggaa gatggtggtc aaattgttcc
    15121 accccacgac aagggaataa ttagtgaagt taaggctatt tcaagcattg agcaggttaa
    15181 ctttaaaggg aacgataacc taattgtcaa agttggtagg gagttagatg agatttacct
    15241 gaatacgctt tcaacacttt cactttcgcc cgatatcatt gaaaagtatc gcgatatgcc
    15301 tattgtttac accccaattc acggaacagg tgtaaacctt gtacccatgg cacttcgcaa
    15361 atttggcttt aaaaatatta tcaccgttga tgagcaatgc gtggttgatg gtaattttcc
    15421 aacagtagca tcgccaaacc ccgaggaacg tgccactatg caaatggcaa ttgacaaagc
    15481 aattaaaaca aatgctgaaa ttgccatggc taccgacccc gatgccgacc gtgttggaat
    15541 tgggattcga aagccaaacg gcgagtttac cctgcttaac ggcaaccaag cggcttcaat
    15601 tcttatctac tatattctca aaaagtggca tgaacataac cgcattaccg gtaatgagtt
    15661 tgtggttaaa acaattgtaa ccactgacct aattagttcc atcgctcatg gatttggggt
    15721 tgattgctac gaggtgctca ccggatttaa atatatagcc gaaaagattc gcgaatttga
    15781 aggggtaaag aaattcatcg ccggtggcga ggagagctac ggttacctgt atggcgattt
    15841 tgtccgcgat aaagatgcgg taatcagctg cgccatgttc gccgagattg cagcttgggc
    15901 taaaagccag agcaaaaccc tgtacgatat tctgagcgat atttacaggg aatatggact
    15961 gtacaaagag catttgatct cacttactcg taagggaata gatggcttag cagagattaa
    16021 agcgatgatg gatgcttttc gaagtaaacc cccacaaacc attggcggtt caaaggtaac
    16081 ttccattatc gactaccaaa atcaagtttt tgtagatgtt cataccggaa aggaaaagca
    16141 tacaaaccag cccaaatccg atgtaatgca gttcatcact gccgatggca ccaaagtatc
    16201 ggttcgccca tcggggactg aacccaaaat aaaattctac ataggtgtta aaggcactat
    16261 ggctgcaaac gatgatattg acacggcgct ggcaaaactc gacaaaaaaa ttgaaactat
    16321 tgtagcagag cttaacctta gatagcaatg aaatcgtatc gcaaagagct atggtttgag
    16381 acacggaatc ggcgtgagta tatcaatatt acacgcgatg ttgaagtttg cctagccgag
    16441 agcggaatta aggaaggact atgcctggta aacgccatgc acattacatc gagcgtgttc
    16501 atcaacgacg acgaaagtgg attgcaccac gattttgaag tatggctcga aaagcttgca
    16561 cccgaaaaac cttactcaca gtacaaacac aacgggtttg aagataatgc cgatgcacac
    16621 ctaaaacgaa ccattatggg acgcgaagtg gttgttgctg ttaccaatgg caaactcgat
    16681 tttggtccct gggaacaaat tttctacggt gaattcgatg gcaaacgccg aaaaagggtt
    16741 ctggttaaga ttattggtga atagttaaaa ggctgtcagt aaatgacagc cttctttatt
    16801 ttcctataag ggttttgtaa tttcctttag ccatttttca tgctcagctg ccaggtgtgg
    16861 cgacagctta aagtaaacca actgatggta atggtttaac cactctcgct cctgaacggt
    16921 tagcatatca aggtcaatgg ctttgatatc aattgggcaa agggtaaggg tatcgaaacg
    16981 taggaatgaa ccaaagtcgt ttgtttcatc ctcaacgcaa acaatcaggt tttcaattct
    17041 aatgccatag gcattgggtc ggtataatcc gggttcattg gacataacca taccgggctc
    17101 aatgggaaga tgattttccg gtctaatctg ttgcggcccc tcatgcacat tcaaaaagta
    17161 tccaacacca tgccctgttc cgtgcccata gtttagcccc tgactccata aagccattcg
    17221 ggcataagta tcgagttgtg aaccacgcgt acccttaggg aactttgcca tagaaagcct
    17281 taccataccc tgcaaaacaa gcgtgaaatc acgcttctcc tgaggggttg ggttaccaag
    17341 gtgaatagtg cgggtaatat cggtagtgcc atttaggtat tgtccaccgg aatcaaccaa
    17401 taggaaacct tcgggtttga tggtgtagct agtttgaggg gttgcgctat aatgcactat
    17461 agccccatga tcggcgtatc ctacaatact tccaaaactc tcactgataa aattcttgcg
    17521 tttgctccta aattccagta acttttcggc aacagtaatc tcaatgacct tacccgattt
    17581 aacattctgc tccaaccagt ataggaactc aaccatggca attccatcct gcaccattgc
    17641 atcgttaata ccctcaacct caacaggttg ttttcgggcc ttaatttggg ttgccaaacc
    17701 agttgtttca ataactttag tgttacctgg aattgctgaa taaattgaga agttaacctt
    17761 ggcagggtct aataccagta catccttctt tttaagcttt gctaacttgt cgaatatttt
    17821 actgtaaggt tttaccgata cctgctcatt ggcaaggaat tgtataacct catcggttag
    17881 cttgttttca tcaacatata gcgttgcttt atcatcgtca acaattgcat atgcaagaaa
    17941 aacaggattg tacgaaatat cggcacccct aatattaaaa gtccagcata tctcatcgag
    18001 tgcgcacatc aggtatgctg tagcatttcg taccagtagc tctttcctta ttcttcccag
    18061 tttctcctgt acggaaacct tggcgaattc agcagtatga acaaaaactt tttgctgagg
    18121 tatgggaggt ctatcgtccc aaattttact cactaaatcg gtatcgaacc ttaccttgaa
    18181 cttttttgct tttaagttct cgcttagggt tctttgattc gagagtgaga taagcagtcc
    18241 attgcaagca actattgagt tttgcttaag gttaagagcc aaccacgatt gaatgtcagg
    18301 cgtttcgggc ataccaattt tacacagctc tatgccagta ccacttagct gcatttcggc
    18361 ttggataaaa taccttgagt cagtccacag ggcagcgttt ttctgggtaa ccactaaggt
    18421 gccagcagaa cctgtaaacc ccgacagcca ttcgcgttcc ttccagtagt ctggaatata
    18481 ctcactaaga tgtggatcgc tatggtggac aatgtaggca tctacatcag cctttttcat
    18541 ttgctttcgg agtagactta atctctcctg aatggttaaa gtcatatctt tttaatgtta
    18601 aactcggaat gggttacaaa aatagcttat tgcaatgatt caggctaaat acatttcaag
    18661 aacatggtgt aaaatgttgg ggtcgatggg ttttgtgatg taaccattac atccagctgc
    18721 ctgagctttt aaacgctcct gtggcgccga gaaggctgtt tgggccacaa caggaatagc
    18781 agaattaact tgttttattt ccatagttgc tgcataacca tccataactg gcatcctaat
    18841 atccataagg atgaggttaa tgccttgatt aagcgaactg actaaatcca ctgcttctct
    18901 accattttta gcccaaatgg ttgacacccc ccatgccgtc agaagttcgt ttaggattaa
    18961 atagttaacc tcttcatctt caacaattaa gactacacta ttcttgaatt caactctctt
    19021 tatggcctgt tcttcgttta gttcatcggc ttggggctga gcctcgttag caattaaacc
    19081 aaatttcacc tcaagcttta acttgccgtc gttgagtatc tccacctcac cctcaagtaa
    19141 ggttaaaagc ctgttggcca acaatatgtt gaaatgggaa gaatggctat aggtgccgtt
    19201 tagcaaggct tttaagtcat cagtaaataa tgttcgtatt tttgagtcaa ttgatgaatc
    19261 cccttcatga ataaaaatct taatcttatc atcctcaatt gaatatccaa tatttaggct
    19321 tccggtttga atgaatttta tgaattgaat gaaatagatg cttagtgcct gatttaaacg
    19381 cccggaatca atgtagagtt caagaaaatc agtattttca ggaattgtat agttaacctt
    19441 tatgttgtag agttccagca accccaatcg aaaatcgata tttgctttta gctcatgaaa
    19501 aaagtcattt acattttgct tggttttaat aactgtgagc gccccagtac taattctgac
    19561 aaaatcctgt aattcattgg tgaggtccaa cagcgatttg ctttggtttt gaataattct
    19621 aaggtattcg gtttgcagcg acttatctag ctcagtaaaa tcgagcatac tgctaaaccc
    19681 aattatagag ttaagcgggg ttcttatgct gtgattaatg tatgttaata gttcatactt
    19741 ctcggcaagc tgttttttta acccatcagc catttggcag gaatccagat tcgctttact
    19801 catatcgata accgttgagg ctactatcga tattctattc aagaattgtg tatcaatcgg
    19861 ttcagaatcg gatgtttgaa atagtatgat tccaccatac agtttgtcgt ccttaacaat
    19921 cccggaaatg tagttcttgc ctatcttaaa ggttcgctca aatactttaa aggttgttgc
    19981 cgatactatg ccatcaagca actcattgaa gggaataatg ttctcctgta cgttggccga
    20041 aattaaggtg gcaagtttct ccttttcaat aaaaagccta cggccaaccg gattccaacc
    20101 cagttcatca ataaaagtat cccacgcttt ggtctttacc ccaaaaattg attccagcgt
    20161 aacaaagcta tcgtcggcac tgtaggaaaa aactatggat attgatttag ggaaagcctt
    20221 ttcaatggtt tggccaagca gtaggaaaat ctcatcgggt gttgtttgcc ctttaatctg
    20281 agagaagaac gatgatatta cctcattctg aaaatcatgt cttttgattt ggttttgaaa
    20341 ctcaacaagc tgactaatat cgcgtgctgc tactataaat ctttttttat cttgaggaat
    20401 atcggcaagt tttgaccagc ttagattaaa ctcgtttcca ctgatatttc tatagattga
    20461 ggtaaaaggc ttccctgaac cactaagcaa ccactcctga aattttttcc aggtatcatc
    20521 ggcaaagcct tccctaagcg aaactgattg tgtattcgat gaatcgaact gaaaaagcct
    20581 tgtggtactt ttgttccagt tcacaacggt gccatccaaa tcaatctcaa gcaaagcaag
    20641 ctgatgctta tgtagtaggt tggcaaaacg ttcgaatgta atggactcat catgggtatt
    20701 tgtatcactc ttgaccgggt caacatttct gaactgtcca ataatgtagt aactgcttgc
    20761 atcggggtta tccttaactg caaatgcaaa aatcctctta tgctgccact cctcacgaat
    20821 ctttaatcta aaatccatct cctggtgtaa ggcagaatta tgtcttaacc tctggaacga
    20881 tttttccagg gactttttat catctctatg aactaatttg aaaaattcat caaggggaat
    20941 gttatcatta tcggattcct taaagcccaa agcattaaca aactttttcc caaaatgaat
    21001 tctattggtg tttgaatccc aaaaccaaaa tcctgcttta tagtatcggt ttaggttcga
    21061 cagaaaatgc ctaacgttaa aaggatagcc taagccaagg ttattgttat ggttctgcag
    21121 cataacgtat ggttataatg actaaggtta tgttaataaa tcctaaattt aaaacgtttt
    21181 gggtaataat caaaatagat cagtgtaagt taaatggcaa aaaggaacta atttgctaca
    21241 agctaaagta ttaaagaggt ttacatatag cagttgttaa aaactttttg ataactgtat
    21301 gaaccaataa tgaaaaccga tgaaaaaagt tggtttattt gcaaaaaagt catttaaccg
    21361 atgaatagcc gacgtagagg gttcaatacc gaaaagctaa aacgggttca tagaaaagag
    21421 atactgttta acaccagcga gttagaagcc attaaccact actgcaagcg gtataaggta
    21481 aggaacaagt caaagttcct gcgtgaggca atcatctcaa aaattttaaa caaattcgat
    21541 caggattacc cccgtttatt ctagaggtat tgctaaaaat tcatttgaaa cctaacatta
    21601 actctgcgcg aagttagata gttgggcacg gcaaaccgat aggagttacc ctcctggttg
    21661 gctacagtct gcacccataa ataggaaacc gtattgttaa agttgaaaag attaaaaata
    21721 tcgaccccta ctgagaggtt agcaattttt aaattaccca ccagtcgttt cccattgtca
    21781 ataaatgatt ttgtgaatcc aacatcaatc ctcttgtacg atggcatcct aaagcttttt
    21841 tgatcaggaa cggagctatc caacattggc aggccactgc cgtatacccc ggtaaggtta
    21901 accctaaagc ttttatcgcc aggcaggtaa tcctgcaaaa ataaactaac ggaaaaggtt
    21961 tgatcggtag ggcggaaata gtatccgggg taaaccgtat tcccaccttc atcggtatat
    22021 gagtcgtaaa gattatcctc ctttgttttg agaatcgaaa ggctgagcca cgattcggct
    22081 ccggaaacca gctcaccatt tatctttagg tcggctcctc gtgcgtatcc ccttgccagg
    22141 ttttcgccgg agtagcgtaa acgaacgtta tcaactgagt atggaattaa acggtttagc
    22201 cacttattat agaactctag gctcattcgg aatggtcggc tccaagccgt aaagtaatac
    22261 tcggctcctg caagtgcatg aatggattgt tgcgctttga tgttcctatt tagctcaccg
    22321 ctagccaaac gaatctccct ataaaaggga ggttgatagt aatacccaaa cgaaaggtga
    22381 tactgtaaac tcctatagtt tgaatgatta aagctgagtg ctaagcgggg gctaaaaaga
    22441 atttccttgt taaaactcca gtggcttacc ctgaatccta tattagttct atactgccaa
    22501 tttccaacgt taaaatgtaa atcggcctga acaaacccta atgcgcggta cgactcaatt
    22561 gttgaggctg ccccaaaaaa tctatcaagc ccaacctgtg agcctgtgta gggtatagcg
    22621 tatcccgatg aatcaaccaa tccccactcc aggagctcat cacttaaaac ttcgcgctgc
    22681 aactgcaccc ccaatttaat taggcttttc ccgaaccaca tatcgccaag caggtttaaa
    22741 ctttgaactt taaggttgat atggttacgg gcatgatcca gaaatcctcc aatcccaaca
    22801 tttatcaggc tatcgccata agtttttgat cccatggagt tatcgagctc attcaggaaa
    22861 tactgtccaa gcaaatcgta tgtttcccgt tcgtttgagt aatatgatga cccaattacc
    22921 ttgacattag aattgggggt taccctaatt gcagcaatta aggcagccat tgacgataaa
    22981 tacctgtctt tctcttggcc atcgtagtat atttttagct gaagggtgtt gttaaaaaca
    23041 ccaaaacggg tttcacgtac ccttggctga aagtcgaaac ggtttatggc cacattgccc
    23101 aacagcccaa tggtaatcct ctcggagagt ttatagtcag ctatgccttg ccaatcgaag
    23161 aacgaaggag tataatcgcc tttggtttcc agtgagttga gtatataacg cgatgtctta
    23221 tagcgcaccc ctgacatgta ggtaagcgag ctgccaatgg caactccttc cagggttgcc
    23281 gaagcaccca gcaaaccggc actgaccctt aacgcattcg cctttggctc acggtaacgc
    23341 acattaagca ccgacgacat cttatcgcca aattcagccg taaacccacc cgatgagaac
    23401 tcaaccgccc caaccatatc ggggtttatg aaacttaaac cctcctgctg agctgagcga
    23461 atcaggaaag gcctgtaaac ctcaacatcg ttaacgtaaa ccaaattttc atcaaaattc
    23521 cctcctctta cactatactg tgagctcagc tcattggacg atgaaactcc gggcaaaagc
    23581 tttaccagtg actccaaccc gcccgacaca ttcggggacg tttttaatcc atcaattcca
    23641 atgcgctcaa tgtttcccac cttccgttgc tgcgaacgaa cgtaaacccc ttcaatatgc
    23701 tcttccgatg gttttaaaat tacctcaagg aacttacccc tttcagcttt aacattcatt
    23761 tcctgtgttt ggtatcccag gcacgaaaac ctaatagata ttgtatcaat atcggttact
    23821 gtgattgaga agcgcccctc atcgtcggtt gaaacaccta aagaacttcc tacaataata
    23881 acgcttgccg agggtatagg catattaagc gtatccttaa caatgccaga tatcttcacc
    23941 cttcgctcct gagcatgtag cgacgcaaaa ccggtgataa atatcaccaa aacacattga
    24001 aagatgtaat atggcggttt tacgctcatt tagccattgg aaatttaacc ccaaaggtag
    24061 taatcgaacc aaacatcgta aaccataaac tcaaaatctt tacctttatt gtgttttttg
    24121 ttattctcgg aaaatcataa ctgaaaaagg atgaaggaaa tacttatagt ttcgcagaac
    24181 aacgcctgcc gttcggccat ggccgaaggt tggtttaaat actatgctcg tggtgcagca
    24241 aatgtaacca gcgcaggaat tgaacctaca caacttgacc ttaacgcagc tcaaagcatg
    24301 atggatgctg taattgatat aaccaagcat caacccaaaa gccttaactc gcttaccgac
    24361 aaggaattcg acattgtttt ggttttggat aataaggtgg caaaaactgc aagcagtaat
    24421 ctgcaatata aaaacctata tgtatttcat ttcgacagcc ctaaagctgc taagagtgac
    24481 gattcgctta cccgtaaagc ctacgataac ctgagagatg acattgagaa ctgggctttc
    24541 gatttcattc accaacacat taaaaggttg atgtagtatg ataaaacaga tagaaatagt
    24601 gcttagacct tacccccaag gttttcatct ggtaactgca gaaatagtaa accagctacc
    24661 tgaactacca ctggtaggtt tacttcacct attcattaag catacatcag cggcactgag
    24721 catcaatgag aatgcagacc ccagcgttag aactgacctg aatgctttct ttaacaggca
    24781 ctaccccatg gagccatact ttaagcacga cctggagggc gatgacgata tgccagctca
    24841 cataaaaacc acagttgtag gaacatcgat atctataccc ataactaacg gaaaattaaa
    24901 cctgggaacc tggcaaggaa tatacctttg tgagttccga aaacacggcg ggagtcgaaa
    24961 aattattgca actgttattg aataaccact caattacaat attttatatt tttcagcatt
    25021 ttactttatt gcgattattg ttttttgtat ttttgctaac aacattaaat tgattaacca
    25081 gcatcaatta caaattgtaa gtttaatgga tgtaagtttt atttactacc tgcataatgt
    25141 agcattgaat gttggctttt taaagcgttt tggcggtcta taagtttatt atttatcact
    25201 taaggcttta ctactcaaaa ttcaacccaa gtaaaacaac aagaaacata ataacaatat
    25261 gaataacaac aagttactac tgttaggcga cgaagcaata gcgcaaggcg caattgatgg
    25321 aggcctttca gggatatacg cttatcctgg cactccatca accgaaataa tggaatacgt
    25381 gcaagcctca aagcaagcag ccgaattgaa tattcacagg caatggtcag ctaatgagaa
    25441 aactgccatg gaggccgcca tcggtatgtc gtatgccggt aaaagggcca tggtatgttt
    25501 taaacatgtt gggcttaacg tagccgccga tgcttttatt aatgcctcca ttaccggggt
    25561 taatggtggg ctaattgtgg tttcagccga tgacccatcg atgcactcct cacagaatga
    25621 acaggattca cgcttttacg gtaagttctc aatgattccc attctggaac catcgaacca
    25681 acaggaggct tacgacatgg catatttcgg atttgaacta tccgaaaggc ttaacgttcc
    25741 tgtattaatg cgtattacta cacgcatggc tcactcaaga gccggggtgc agcgtcgcga
    25801 gtcaagaccc caaaatgaaa tccgactgcc atcggatcca cgccagtttg ttttgcttcc
    25861 ctccattgct cgcaagaact acaaaaaact gctggtttcc caaactgcaa tggaacttga
    25921 gtctgaaaaa tcggcctata accagatgat tgagggaacc gataaaagtt taggtataat
    25981 aacaactggc atagcttaca attaccttat cgagaatttc cctgaccata aatgtccata
    26041 tccggttctt aaaatttgcc agtacccaat accacgcaaa caggttactg aactctataa
    26101 cagctgtagt aagcttttga tacttgagga aggatacccc cttgttgagg aaatgataaa
    26161 aggtttccta aacattggca aaaccattca cggacgactt gatggaacat taccccgcga
    26221 tggcgaactt aaccccaaca ttgttgccaa agcccttggg ctaacggtta ctgaggggga
    26281 gcctgtgccc gaggttgtta aaaaccgtcc cccatcactc tgtcagggtt gtgggcatag
    26341 ggatgtttac gaggatttga atgaggcact aaaggaatac ggaacgggaa gggttttctc
    26401 cgatattggt tgctacaccc tgggtgcgct gcctccattt aatgctatta actcctgtgt
    26461 tgatatgggt gcctccatta ccatggctaa aggtgctgcc gatgcaggtc ttgttccagc
    26521 ggttgctgtt attggcgatt caacctttac acatagcgga atgaccggcc ttttagacgc
    26581 tgttgtagag aactcgccca tcacagttat tatttctgac aactcaacaa ccggaatgac
    26641 aggaggccag cactcttcgg ctaccggacg aatagttgaa atatgtaaag gaattggcgt
    26701 tcaccctgac cacataaggg ttgtaacccc cttaaagaaa aatcacgaag agaacgtgaa
    26761 aatcattaag gaagagctgg cttacaatgg cgtttcggtt attattcctc agcgtgaatg
    26821 tatccagacc tttgcccgac gcaaaaaggc tgagaagaaa gagactaatg agtaacataa
    26881 aacgacttca atcatgaaaa acgacataat acttgcaggc gtaggcggac agggaatact
    26941 ttccattgca gcagccattg gacttgccgc cgtaaacagc aatctctaca tcaaacaatc
    27001 cgaggtgcac ggtatgagcc aacgtggcgg cgacgtgcag tcgcaccttc gaatatccga
    27061 taagccaatt gcctccgacc tcattccggc tggcaaagcc gatatgatta tttctgttga
    27121 acccatggag ggcctcaggt atttaccctt tttggcccaa acaggctggt tagtaaccaa
    27181 tgttaccccc tttaacaaca tccccgatta ccccaatatc aacgatatac tatctaaaat
    27241 taaagctgta aagaaccaca ttgctattga tgccgatgcc attgctaagg aagcaggctc
    27301 aacaaaggct tcaaacatag taatgcttgg cgctgcctcg ctattcctcg atattccttt
    27361 cgaaaaaatc gaagaaggaa tacgccagct ttttggtaga aaaggcgacg aggtggttca
    27421 gctaaacctt aaggcactcc ggttagggcg tgagttcagc gaaaaaaaca aataatttga
    27481 gtgattgatt taaaagccac cctccaggtg gctttttttt tagattaacc tttagtattt
    27541 tacgctaaat ttaatccaac ttcaaatgga aaatttaggg tttcatctcc caattagtga
    27601 acccattaca gtatttggaa ttaccctttt ggtgattttt attgtccctt tagttttttc
    27661 aaagctaaaa cttcctggca tcattggtct tattattgca ggcatgatac tgggaacatc
    27721 tggactaaac attcttgata aggagagaag tattgaacta cttggaaacg taggactaat
    27781 atatttaatg tttttggccg gactagaatt agaggttgga agttttttaa aaaatcaacg
    27841 caagaacatc acttttggtc tttttacctt tacaattcct ttcctatttg gttttactat
    27901 ctgcaagtat tttctggggt ttggttattt gccatcgctc ctgattgcaa gcatgttctc
    27961 aactcaaacc atgatttcat tcccaatagt ttcgcgcctt cgactcacaa aaacattgcc
    28021 ggttgaaata tcaattggtg gaacaataat aaccgatacc ctagttcttc tcatttttgt
    28081 tatcataaca tcgttatcag caaatagcgg tttaaatgga ttccttctgc ttatacccaa
    28141 gatactggca tttgtccttt ttatcattct attacttcct cgatttaccc actggtttct
    28201 gagaaacctt tcatccgacc aaatggctca atacgttttt gttcttgcaa cagtatttac
    28261 taccagtgca attgcacggt ttttaggaat tgaacccata ataggggctt tcctggcagg
    28321 aatagtactt aacaggcaaa ttccaccctc agccatgctc atgaaccgta tcgagtttat
    28381 tggaaacgcc ctatttatcc ctatctttct tttttacgtt ggaatgctaa ttgacctaaa
    28441 agcttttgta agcggatacg aatcgcttga actatctgca attctcatat cattcgccat
    28501 aatctcaaaa tggttggcgg catacctcac tcaaaaaata tttaagctaa aaacactcga
    28561 tcggcaacta atttttggtt tgagcaccgc tcacgcggcc gctaccatag caattattct
    28621 ggttggttac aagattggta ttgtggatat taatgtgttg aacgccacta ttgtggtgat
    28681 ttttgcctcg tgtgttgtga gtgcaatggt aaccgaaagg gctgcaagga
//
LOCUS       DAOOWJ010000024        28642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1654_length_28642_cov_7.414594, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000024 DAOOWJ010000000
VERSION     DAOOWJ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 28642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28642
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1654_length_28642_cov_7.414594"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLW22_04025"
     CDS             complement(<1..537)
                     /locus_tag="PLW22_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU73906.1"
                     /translation="MVPNYTIYFYNRKFIIASNLNEELFAAPSFFCWGKTEPDDIPKL
                     IDFFQSHIEVSTLFACTSDTNTAMKHFTNNFTHIEAAGGVVCNGKNEILLIKRFGRWD
                     LPKGKVEPNEPIPIAALREVTEETGVKNISLGEQITTTYHTYSLHGKQMLKSTHWFKM
                     YTQTNDALVPQTEEGIEQV"
     gene            536..1165
                     /gene="pyrE"
                     /locus_tag="PLW22_04030"
     CDS             536..1165
                     /gene="pyrE"
                     /locus_tag="PLW22_04030"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOU73907.1"
                     /translation="MDSKVIANKLLQINAIKLNPANPFTWASGWKSPIYCDNRKTLSF
                     PEVRNAICDGFVKAIKERYPQVEVIAGVATGAIAHGMLVADRLNLPFIYVRSAPKSHG
                     LANQIEGDYKPGQRVVVIEDLISTGMSSLAAVDALRDAHCEVLGLLAIFSYQFDQAVS
                     SFAKAGVEFATLSNYSVLIEAVLEQGSITPTELELLKSWRQNPEEWGRR"
     gene            complement(1184..1885)
                     /locus_tag="PLW22_04035"
     CDS             complement(1184..1885)
                     /locus_tag="PLW22_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73908.1"
                     /translation="MKQNIFSILKSVTLLVVITYSNQGALYAQNQDCKVLLPEISAAY
                     TGKCKNGLAHGKGKAWGTDSYEGKFKNGLPHGFGIYTWANGDKYEGNFYNGQMDGKGI
                     FKGKINGKDSVYTGFWQKGVLHHKILPPKYQIINARNVQRYTITKTGSENRILIAFTQ
                     NGTTNNLVTNLQIVCDTGSPFKLGEKSGFENILFPVNCKITYQTPNALRTVWYDVTFE
                     FTINEAGEWAINLFN"
     gene            1974..2046
                     /locus_tag="PLW22_04040"
     tRNA            1974..2046
                     /locus_tag="PLW22_04040"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2007..2009,aa:Phe,seq:gaa)
     gene            complement(2072..2575)
                     /locus_tag="PLW22_04045"
     CDS             complement(2072..2575)
                     /locus_tag="PLW22_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73909.1"
                     /translation="MEKLKMIFALLMGLAVIVSCKQETKKQAATTDEIVKQSIKSWME
                     NRKDEYPNYVPLEFGELTPRYRFNSRTHSIEIALNTEKSKDNPNQNTLDSLKRLLNEN
                     STDFLGYTITHQFTTKGIDGEVKKDEKLFFIDPQNRVITVLNSDAWDLIMDKELFFRP
                     ESPDSVK"
     gene            complement(2720..4084)
                     /locus_tag="PLW22_04050"
     CDS             complement(2720..4084)
                     /locus_tag="PLW22_04050"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOU73910.1"
                     /translation="MDTIKAYIEENKQRFLDELFGLLRIPSISSISEHKPDMVRGAEY
                     WRETLLKAGADRAEVMPTPGNPVVYGEKIIDPKLPTVLVYAHYDVMPVDPIELWKSQP
                     FEPEIRDGKIYARGADDDKGQGFMHAKAFELMVKTNTLPCNVKFMIEGEEEIGSPNLG
                     KWCQDNKEMLKADVILVSDTSMIGKDVPSITTGLRGLAYVEVEVTGPNRDLHSGLFGG
                     AVANPANILAKMIASLHDENNRVTIPGFYDDVLEVSPEERADMARAPFNLDDYKKALD
                     IDDIHGEAGYTTMERTGIRPTLDVNGIWGGYIGEGAKTVLPSKAYAKISMRLVPNQNH
                     EKIAQLFKKHFESIAPKSVKVKVTPHHGGQGYVAPTNTPAYRAASRAIEDVYGIKPVP
                     FRSGGSIPIISTFEQVLGIKSILLGFGLESDAIHSPNENYPLEQFFKGIEVIPRFYKY
                     FSEK"
     gene            complement(4106..4681)
                     /locus_tag="PLW22_04055"
     CDS             complement(4106..4681)
                     /locus_tag="PLW22_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOU73911.1"
                     /translation="MIEIDNKVVSSEILEQRFCCNLPRCLGKCCVHGDSGAPLTPNEA
                     LTLEQILDKVKPYMTPQGIATVQEQGVALTDSDGDLVTPLIDGKECAFTVFENGIATC
                     AIEKAWNNGIVDFRKPISCHLYPIRVKEYSTFTAINYHQWDVCAPARELGEQIDLPVY
                     RFLKDAIIRAYGKEFYGQLEDAAKLLSLRTE"
     gene            complement(4697..6220)
                     /gene="gpmI"
                     /locus_tag="PLW22_04060"
     CDS             complement(4697..6220)
                     /gene="gpmI"
                     /locus_tag="PLW22_04060"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOU73912.1"
                     /translation="MIKSKVLLMILDGWGIGNKDKSDVIYSTPTPNIDRLTSKYPNAQ
                     LLTSGENVGLPDGQMGNSEVGHLNIGAGRIVYQDLVRINKDIKEGKLAQMPVLVNALQ
                     YAKQNNKAVHFIGLVSDGGVHSLDKHLYKLCDITKEYGLSKVYIHALTDGRDTDPQSG
                     LGFVKSLEDHLKSSNGVIASLVGRYYTMDRDKRWERVKEGYDLMVHGKGKPTTDIVRA
                     IEESYAEGVTDEFIKPIVRVDAEGKPVGLIREGDVVICFNFRTDRLREITIVLTQKDM
                     PEFGMQTMPLHYVTMTRYDDTFKNVNVIYEKDNLLNTLGEVVSNQNLKQLRIAETEKY
                     AHVTFFFSGGREQEFPGESRILIPSPKVATYDLQPEMSAPLVKDAIVTELHKKTHDFI
                     CLNFANGDMVGHTGVYTAIQKAVQTVDACVGEVVDAATANGYEVVIIADHGNADYALN
                     PDGSPNTAHSLNPVPIILVSDRFKKVDNGILADVAPTILTMMGLPIPQEMTGTVLCQ"
     gene            6498..9383
                     /locus_tag="PLW22_04065"
     CDS             6498..9383
                     /locus_tag="PLW22_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOU73913.1"
                     /translation="MRKLIFIAFAIFATNAFSQINTAGLPIIKHYPKSVYGAGTQNWS
                     ICQDSRGFLYFANNNGLLRYDGNSWDLFPLPNNQLVRSVYCSGDTIFVGAFEEIGYFV
                     YNRQADMVYHSLISLVPKTDVNFDEAWRIFKIGPKYIFQTFSHILEYSKGEMRVFRAP
                     ELLQFSFVMRGNYYVQGKEGNIYRYQNGSFDRFNDNGIFNGKQIWACFEISGGKTLLA
                     TINHGVWIFDGITFTPWKGQVNDLLKAYQVFSASRVSGGYIAFGTIQNGLIITDDQGN
                     LVKFINKSTGLQNNTILSTFVDNANNLWLGLDNGIDYIELNSPLSFIGEGLGLEGAVY
                     ATKIFDGKIFVGTNQGLYVASWPIGVFVDGTKPFRMVPLTKGQVWSLFESDGKLYCGH
                     NFGTFIIDKGLNVRLISPEEGSWMFLPIRSVPDLMIVGKYKGLHLYSKANGEWRYLKK
                     IAGFNESSRLLVEDDNESIWMAHGYKGIFKLMLNQSKDSIRFSTLYDNTRGFPGKFAI
                     NVEKIGNQVVFLTSQGVYRYNSLTDNMEPYQELNQIIGDATGVRRMVEDEKGNIWILK
                     SNGLLKLVREADGAKRVYQTPVSRFGANLVASFENIYVYNHSNIFIGTENGLVYYNSL
                     YNPELQKNELYCAVKSISSTGKHESVIFNDYAVTGNGGLYIPFNQNSIRVTAAAPVYN
                     AGEITFSFLLKGYDNDWSPWQRDNAKDFSQIPYGEYNLMVRVRDAMGRISEPTVVHIQ
                     VGLPWYFTWYAFILYLVLLVAAFYTLRILVRRKVQRATARVKEQKNLELKVKEEEHKR
                     QVLEAEREIIRLKTENLQVQIEHKNRELAATAMQIAHKNDFLNRLRLRLEVIAKSINP
                     VSQKEVLDLIRNIDNDLKMDKEWQRFEHHFDEVHSGFIKKLKEMYPELTPSELRLCAY
                     LRLNMTTKDIAQILNISVRGVEISRYRLRKKLKIESDTNLVDFMLNL"
     gene            complement(9386..11206)
                     /locus_tag="PLW22_04070"
     CDS             complement(9386..11206)
                     /locus_tag="PLW22_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOU73914.1"
                     /translation="MSRKFINSIIALLLVVPFTSLAQVKVLVWNDEFNYTGLPATDKW
                     GYDVGGGGWGNNELQYYTNARTENARVENGNLVIEARKEQYEGSEYTSARLVTRYKGD
                     WLYGRVETYAKLPSGTGTWPAIWMLPTNWVYGSWPKSGEIDIMEYVGYDPGVVHGSIH
                     TEAYNHVLGTQKTATISVPDAETAFHLYALEWTPEKIDIYVDNNKYFTFDNEHKDYKT
                     WPFDQVFHLILNIAVGGNWGGAQGVDPNIWPQKMYVDYVRVYQYIDVSALKVSGPEYV
                     SPNQTGVTFSIQNVADATFSWSVPAGATIVSGQGTNSIVVNWGDVQGDVSVEITHPQA
                     GGTYTKTVKTAVIPTGDRFSLLNVKENGLIGWAGLNTSPNTITLSAVDTLLKVKYNIT
                     KPEDFPYFTYTFQNPVDMSNLTVLNVEMMTYNQSKSVVMRADLFDTDGRLTDVSPVFR
                     FYPVEPHGVFHYYSFDFNNNWGSNTPEYGKQVNYHQIAGVRFYVNYGVYGKAASDSLW
                     ISDVVLSNTLLTIFSPSENKISRFDIYPNPASTYIKINSSALKGNVKIQVINIAGVLV
                     KEIIENSDGQINISDLPNGFYSVRIITHTNLLQTGSFIKL"
     gene            complement(11322..13568)
                     /locus_tag="PLW22_04075"
     CDS             complement(11322..13568)
                     /locus_tag="PLW22_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU73915.1"
                     /translation="MMRLKLIIAVVTLAAWSCSGNKATMDHSGGKLSIDPAIERKVDS
                     VLKLMTIDEKIGQLNQLTGNGETTGPITFATEYQDAIRKGEVGSMLNVNGAAYTYKVQ
                     KIAVEESRLGIPLIFGYDVIHGYKTIFPIPLGEAACWDLDAIEKSARIAAIEAAAAGQ
                     HWTFAPMVDIARDPRWGRVMEGAGEDPFYGSLVAKARVKGFQGSSLADPSTIVACAKH
                     FVAYGAAMGGRDYNTVDISNRTLHEVYLPPFKAAIDAGVGTFMSSFNELNGVPVTGNK
                     EMVKDILKDEWQFNGFIVSDWGSIREMLVHGIAADDYQAADLAMNAGIDMDMEGHIYI
                     HQLKKLLADGKITEKQIDDAVRRILRIKFMLGLFDDPYRYCDTIREKELILNPRHLEI
                     AREVARKSMVLLKNDGVLPLSKKLNTVAVIGPLADNTEDIIGTWSARGEGKDAVSVLT
                     GIRQKLPNTKVIHAKGCDITGDSKAGLTEAIATARKADAVVLVVGEAAMMSGEALSRA
                     FLDLPGVQKDLVLAINELKKPMVVVLMNGRPLTIEWMDNAVPAILEAWFPGTMGGPAV
                     ADVLFGDYNPSGKLPITFPRNVGQIPLFYYHKNTGRPRVETERYTSKYIDSPNTPLYP
                     FGYGLSYTSFDYSNFGVSTNEIGMTDTLKVWVDVTNTGNYDGEEVVQLYIRDMVGSVT
                     RPVKELKGFEKVFIKKGETVKVMFKLTASDLAFYTRTMEFKAEPGDFKVFVGGNSRDL
                     QELSFRLK"
     gene            complement(13630..15390)
                     /locus_tag="PLW22_04080"
     CDS             complement(13630..15390)
                     /locus_tag="PLW22_04080"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOU73916.1"
                     /translation="MNKRHIIFSALVLVGLTWFGCEPMELDKPNIGNAPTAEQLDFTI
                     TPGDDAFHFVITNTSTVKGIANWDLGNGIKAVGNSVTAYYPLAKTYNITLTLYASGGS
                     TSKTKEFEQTETDWSYLESPIITALTGGVDAPNGKTWVIDSLSEAHLGVGPADSYSPV
                     WWAAAPLAKSGHYLYDDEFTFKLVGFTYTIDTHGKTHVNHDGNADEDGLSAGYYVSSF
                     WNDAYDIDMNTNDAARGELTWSISEENGKYYIQISSQSGNISYDDGNPRKYEVLEWNE
                     NFFHLRSVGGAARYHKIIPKGYVRPTISYTLNVTATANPNEYTFALEDLTVPSSFTIS
                     NITYDFGDGSSYQTTNGAEVVTHTYMRKGNYNTKVTVTANGQDFVKTSTVAVAANHPT
                     YQEYLLDAMVMYNDFGETTLVPMQVDQSDGGATLEIVTNPNNTLYPNRSAHCALFTKY
                     NSQWANAFTRLPDGYRFDLTKQTVFKVLVYGKAGDQVLLKLENTDRGGNAWQTGTHDL
                     IYTIQNDNKWEIAEFNFAGVGAGWDWTGDIFTGDVTTDSRFNKDFYNVVRIMINPGNN
                     GGTFSVHLDDLAGPHIEGLK"
     gene            complement(15407..16990)
                     /locus_tag="PLW22_04085"
     CDS             complement(15407..16990)
                     /locus_tag="PLW22_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOU73917.1"
                     /translation="MRKIKNISIILFMALGIVACTKDFLELQPLTDRVEDNFYKTEKD
                     AFEAVVSIYDVLQWQTGGGYHPWDLVSNILSDDAFAGGSGPGDRPGLVRMGKLSNFVT
                     DEEPLGIWKDRFSGIYRANLFLSKVDGVDFKTEGLKTRLTAEARFLRGYFYFELVQFY
                     GNVPLILKPLAPSEYQQSAADPADVYNQIASDLYYAYQNLPATITAAEKGRASKWAAG
                     ALLARVYLFHKGYGKGVLNITTDLKADDKTFTETDIETIIDDIVENSGHGLVANYANL
                     WGVDNENNNESIFEIQHTQKADWGDWGWRNGTEGNWAVVMTGFRGVEDPIYESGWSFQ
                     PASQSLADEFEPGDSRYSVSILDAAAESLVYKPENCYQHTGYAFKKFYPRKADRPATN
                     DALNWPYNRPVIRFADVLLMGAEPYLDNNLGKAQDYYSRVRKRAFGDAHVAPTLTNDQ
                     AGLDLIYHERRVEFAGEGLRYWDLLRRGLTYAETKINAAAGASPLDETFSSATKGLLP
                     IPQTEITLSGNKLKQNAGY"
     gene            complement(17011..18474)
                     /locus_tag="PLW22_04090"
     CDS             complement(17011..18474)
                     /locus_tag="PLW22_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU73918.1"
                     /translation="MFNDFEHAWINNGTDEDSYRSWGGADEHALLSYFGRVNYDFDGK
                     YVFEGVLRADGSSNFGSNNRFGYFPAFSAGWVMSKEEFLQGFTPLSFLKLRFGWGRNG
                     NEAIGAFKYTSLIGSGSKYTFGTDEVITLGSNPVGISNPDLKWETSEQTNVGVDARFL
                     SDKLTFTLDLYNKKTKDLLVVAPIPGYIGNAAPTVNGGSVSNKGIEFELGYKTLINDL
                     GINANLSGAYNKNEVLSINNSEGKIYGAGLAVGMYNICVMETGYPIAYFWGYKTAGVF
                     QNEQQIQNYKSSDGTVIQPNAKPGDLIWVDKNDDGTIDDKDRTMIGNPYPDFTLGINL
                     GLTYKDFDFNMFWYGAFGQEIFKGTRRYDLPMSNWDASVLERWTGEGTSNSHPRVTIN
                     DPNQNYFRVSDFYIEDGSFLRLKNITLGYTLPQKFSQKVKISKLRIYATAVNLLTFTK
                     YSGFDPEIGAKWALDVGIDRNIYPQPRTFMFGVNLSF"
     gene            complement(18474..20048)
                     /locus_tag="PLW22_04095"
     CDS             complement(18474..20048)
                     /locus_tag="PLW22_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU73919.1"
                     /translation="MKKIFGVISLILWCGSLLAQEINVSGTVTSSDGATLPGVTIVVK
                     ENPTKGAITDANGKYTISVLPNQTLEFSFVGMQTQTVPVGGRTQIDVVLQESTQMIEE
                     LIVVGYGVQKKSLVSSSVSKVSSEDIEKAAPTRVEQVLQGRTAGVQVIANSGQPGEGF
                     TVRIRGVGTNGTSDPLYIVDGMPVGGIDYLNPNDIQSVEILKDASASAIYGARGANGV
                     VLITTKQGQKGKFSVNYDYSMGYQNAWRKVSLLNAKEYAIIMNESYANDNRPIPFPDV
                     NAVVQAVGNGTDWQDELFYKNAPTYNHQFSVSGGNENSTFLSSLSYTYQDGIVAKGKS
                     NYERYTYRLNSTHKIGKFSMGNNLAYTNKVTRGIDPNAEFGGPLAKAVNMDPITPVKN
                     PDGTWGISNYATQEVVNPVAYLSVLNSNYSENKLVGNVWGELEIIKGLKAKTNFSIDY
                     ANGSSRTFVPVYFLSDIVKATTSEASGTVNKWFTWQSENTLTYNNTIGQHNFAAMVGM
                     TANNTTMSILEVRKTT"
     gene            20369..20971
                     /locus_tag="PLW22_04100"
     CDS             20369..20971
                     /locus_tag="PLW22_04100"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOU73920.1"
                     /translation="MKRFSGTVLVAFLVAAAVKDVGAQAPSADSLILYPTIILDGEYV
                     PHIKIEDVVKVGKRRFKSRREMSQYYRMIYNLKKTYPYAQIAKYKLLEINENLKTLKT
                     DREKKEYIDKAEKELRNQFEKDLTKLTISQGKMLIKLIDRETGRTSYELVKELKGGFS
                     ATFWQGIARLFGSNLKTKFDPQGEDKILNELIFLYEQGLI"
     gene            complement(20976..21437)
                     /locus_tag="PLW22_04105"
     CDS             complement(20976..21437)
                     /locus_tag="PLW22_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73921.1"
                     /translation="MKKLALLATIIATMFSYSCLNRCDVDCVNPPSPLWLKVVDKTTL
                     QNLIAEGFYHRDTIKIYYFDGVTKKYANIYYVGPVGQNDIIQTNIGILQPFEQLNTFY
                     LYLNQYDTDTILVRYVKSSDGCCTAYPLDSIAINGKYVDVDNVDYSFLIKK"
     gene            complement(21440..22096)
                     /gene="elbB"
                     /locus_tag="PLW22_04110"
     CDS             complement(21440..22096)
                     /gene="elbB"
                     /locus_tag="PLW22_04110"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOU73922.1"
                     /translation="MGNNKKFAVILSGSGVFDGSEIHEAVMTLYAIAKHGATYEIFAP
                     NINQYHVINHIDGSVMNEKRNVLVESARIARGKIKPLNEFNPELFNALIIPGGFGVAK
                     NLSTWAFDGPKCSIHPQVEAAIKGMHKLKKPIGALCIAPVLLAKIIGNATLTIGKDTS
                     TAQAIESLGAKHQPTGHAQVVVDEKNMLFTTPCYMLDANIVDISNGAKAVVSEILKWI
                     "
     gene            22172..22738
                     /gene="thpR"
                     /locus_tag="PLW22_04115"
     CDS             22172..22738
                     /gene="thpR"
                     /locus_tag="PLW22_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOU73923.1"
                     /translation="METKRTFLAIDVNPQDNIINLLQELKRNHKLDSIKWVDPAIMHL
                     TIFFLGDTPSAQIPSICEMLKATIKKFEPFSIILKGIGYFGRPSPRVIWVGVDFPKLL
                     NELKLSVDKVLSEFDFSDDKEKFNPHLTLGRVKFMHNSDKLNAAIQQYRNKSFQVQEV
                     KELVLYESILKPQGPEYRAIQKFELKGC"
     gene            complement(22735..24141)
                     /locus_tag="PLW22_04120"
     CDS             complement(22735..24141)
                     /locus_tag="PLW22_04120"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU73924.1"
                     /translation="MNKKKQAFRYLLFDVAGSALAWTLFNLYRKRVIESNLFGIDIRF
                     NPDYKFYLGLVFFTLFWVSMFGLVGLYHDPYRKSRLREFGASLTITIIGTIVLFFVLL
                     IDDYVVAYQNYYKILLAMFLLVFALTYTPRLVITTQTIHRIHNRKIGYPTIIVGSGKK
                     AMQTYNDINSEKISQGYRIIGFIDLPNGVRVDQSLILGSVNNIPEVVARYGIEEVILA
                     IDNESPQTLTTVLNLLVPLNVNIKAIPSMYDILLGKVKLNRIIGTTLVDVSFEPMPAW
                     QANLKEMLDYFIAIVGLLLSAPVALILMAVIKTTSKGPVIFKQERIGQHGKPFHIYKF
                     RSMYVDAEANGPALSNQTDPRVTPVGRFMRKTRLDEIPNFINVLKGEMSIVGPRPERQ
                     FYIDQLVKVAPHYLHLQKVKPGITSWGQVKYGYAENIEQMVERLKYDLLYLENMSLYI
                     DFKIMIYTILTIFKGKGV"
     gene            complement(24188..24991)
                     /locus_tag="PLW22_04125"
     CDS             complement(24188..24991)
                     /locus_tag="PLW22_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73925.1"
                     /translation="MTTCLVTDSNKLIQYQGENFFNNTIEANNQLIDKILLGICESNS
                     QIARLFNYPELLVVDTNPKTSYTIIANALRAGINVLTPSLPHFSSEKIQELITIAQEI
                     GVEFGFLPKYSLELPSTHSPIIIDSVKETINLTSTENLLQQIANDLIFLLSPIKSELR
                     KVRVYWLPLYSHPVSTLKLIVDFNDNSLITYQIKNKKETSMLKVVLTTEEYDKTFNIF
                     EAQIKPESTKETVMQNVDYFINQRKMLYPAAMALKSKQIIEIIIKKIYS"
     gene            25127..25792
                     /locus_tag="PLW22_04130"
     CDS             25127..25792
                     /locus_tag="PLW22_04130"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOU73926.1"
                     /translation="MILKNMLQDDSIRHKGLRRKLVEEVAAKGISDKRVLEAMLKVPR
                     HFFMDSGFLSFAYKDQAFPIGSGQTISQPFTVAFQTQLLQVSPMQKVLEVGTGSGYQA
                     AILMEMGARLYTIERHRELFLAAKALLEEMGYRPSCHYGDGYQGLPPYSPFDRIIVTA
                     GATEVPQALVDQLAPGGRLVIPVGNDYGQTMMLIEKLDDGTIKESAHGNFIFVPLLKG
                     KAK"
     gene            25800..26435
                     /locus_tag="PLW22_04135"
     CDS             25800..26435
                     /locus_tag="PLW22_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU73927.1"
                     /translation="MIAIKQFVFNPFQENTYVLYQPQGEAIVVDAGCSTESEFQELLD
                     FVHSQNLTVKCLINTHCHIDHILGIDKLKKQFGVKAFAHIDDFPLLQMAPAHAMMFWL
                     YLETVPAIDETLSHGDMIELNGDSIKVIHTPGHSRGGLCFYAEQERFILTGDTLFNLS
                     IGRTDLAGGNYETLIKSIREQLMVLPDDVIVYPGHGNSTTIGIERASNPFL"
     gene            26537..>28642
                     /gene="gcvP"
                     /locus_tag="PLW22_04140"
     CDS             26537..>28642
                     /gene="gcvP"
                     /locus_tag="PLW22_04140"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOU73928.1"
                     /translation="MYTDKFVSRHNGPSDHEVGEMLKVIGVSSVDELINQTIPANIRL
                     KKPLNLPAPMSEYEFLTHMHKVASKNKLYKSFIGMGYYGTVTPSVILRNIFENPGWYT
                     SYTPYQAEISQGRLEALLNFQTMVVELTGMQIANASLLDEATAAAEAMLMMLNLRSRE
                     AVKAGKNVIFVDQDIFPQSLDVIKTRSNPQGIEVVTGCYSEYNFTGREFGVIVQYPSA
                     KGNIRDYAAFAAKAHEAGALVTAVSDILSLALITPPGEWGADIACGSTQRFGIPMGFG
                     GPHAAYFACKDEFKRSMPGRIIGVSIDRHGNKALRMALQTREQHIKRERATSNICTAQ
                     ALLATMAGMYAVYHGPEGIRRIASYVHSTAVQVAKELVGMGYKQNVKNIFDTLEVELP
                     SGVTIDDIRKRALAREINLNYKPNGTVGISFDETTSVTDVNNLLSVFAESAGKKFNSI
                     AEIKETIEIPENLRRKTPFLTDKVFNSFHSETEMMRYIKKLERRDIALNHSMISLGSC
                     TMKLNPATTMLPLSWPEWNSLHPFVPADQAEGYMQVIRELEQYLAEITGFHAVTLQPN
                     SGAAGEYTGLMVIRQYHINRGEGHRNVVLIPSSAHGTNPASAAMAGMQVVVVACDEKG
                     NINVDDLRAKAEQHKQNLSAFMVTYPSTHGVFESKIREMMDIVHQNGGLVYMDGANMN
                     AQVGLTSPGEIGADVCHLNL"
BASE COUNT         7739 a         6032 c         6383 g         8488 t
ORIGIN      
        1 aacctgttca attccctctt cggtttgagg tactaaagca tcgttggttt gagtgtacat
       61 cttaaaccaa tgggtggatt ttagcatttg cttgccatgc aaagagtagg tgtggtaagt
      121 ggttgtgatt tgttcgccaa gcgagatgtt tttaacccct gtttcttcag taacttcgcg
      181 taaagcggca atgggaatgg gttcattagg ttctactttg cctttaggta aatcccagcg
      241 gccaaaacgc ttaatgagta gaatctcatt ctttccattg catacaactc cacctgctgc
      301 ctcaatgtgg gtaaagttgt tagtgaaatg tttcattgct gtattggtat cggaggtgca
      361 ggcaaaaagt gtggagacct cgatatggct ttggaaaaaa tcgattaact ttgggatgtc
      421 gtcaggctcg gtcttccccc agcagaaaaa cgatggggcg gcaaaaagct cttcgtttag
      481 gtttgaagcg attataaact ttcgattgta aaagtaaatt gtatagttag gaaccatgga
      541 cagcaaagtt attgccaata aactactgca aataaacgca ataaagctca atcctgcaaa
      601 tccgtttaca tgggcatcag gatggaaatc acccatttat tgtgataacc gaaaaacatt
      661 atcgtttcca gaggttcgca atgccatttg cgatggcttt gttaaggcaa taaaggagag
      721 gtatcctcag gttgaggtta ttgctggggt tgccaccgga gccattgcgc atggaatgct
      781 tgtagccgac aggctaaatc taccttttat ttacgtccgt tcagcgccca aatcgcatgg
      841 tttggcaaat caaatcgaag gcgattataa acccggccaa agggtagttg taattgaaga
      901 cttaatatca accggaatga gcagccttgc ggctgttgat gctcttcgcg atgcccattg
      961 tgaggttctt ggcttgctgg ccattttttc gtatcagttc gatcaggcag taagtagctt
     1021 tgctaaagct ggtgttgagt ttgcaacact atcgaactac tcagtgttaa ttgaggcagt
     1081 attagaacag ggtagcatta ccccaacaga acttgagctg cttaaaagtt ggcgacaaaa
     1141 ccctgaggag tggggcaggc gttgagccag ttggcgatat cgcctaatta aatagattta
     1201 ttgcccattc accggcttcg tttattgtaa attcaaaggt tacatcgtac caaacggttc
     1261 tgagtgcatt gggtgtttga tatgttattt tacagtttac tgggaaaagg atgttttcaa
     1321 agccggattt ctcgcctaat ttaaatgggg agcctgtatc gcaaacaatt tgaaggttgg
     1381 tcacaaggtt gtttgtagtt ccgttctggg taaatgcaat tagaatacgg ttttccgaac
     1441 cggttttagt tatggtataa cgctgcacat tccttgcatt tataatttgg tacttaggag
     1501 gtaagatttt atggtgtagt actccttttt gccaaaagcc tgtatatact gagtctttcc
     1561 cgttgatttt gcccttaaag ataccctttc cgtccatttg gccattataa aagttaccct
     1621 cgtacttatc gccatttgcc caggtgtata taccaaaacc atgaggtagt ccattcttga
     1681 actttccctc gtagctatca gtaccccatg ccttgccttt tccgtgagcc aaaccatttt
     1741 tacactttcc tgtgtaagct gccgatattt cagggagaag cactttgcaa tcctgatttt
     1801 gagcataaag agccccttga ttactgtagg tgataacaac aagtagtgtt acacttttta
     1861 ggatggaaaa aatattctgt ttcatagcca tttagggtgt ttttgatttt tttttagttg
     1921 atgttttgca cgaataaaaa attgaatata tatttgcacc gcaattgagc gctggtgcca
     1981 tagctcagtt ggtagagcaa aggactgaaa atccttgtgt ccgtggttcg attccacgtg
     2041 gcaccacagc ccccggtaaa tgggggcttt tttatttcac actatcggga ctttcgggcc
     2101 taaagaatag ttccttatcc attatcaaat cccatgcatc ggagtttaga actgttatta
     2161 cacggttttg cgggtcaata aagaaaagtt tttcatcctt tttcacctca ccatcaattc
     2221 cctttgtggt gaattggtgt gttatggtat aacccaaaaa gtcggtgcta ttttcattta
     2281 gtagcctttt taggctatcg agagtattct ggtttgggtt atcctttgat ttctcagtat
     2341 ttagagcaat ctctatgcta tgggttcggc tgttgaaccg gtagcgtggg gttagctcgc
     2401 caaactccaa tggcacatag ttggggtact cgtcttttcg gttttccatc caactcttta
     2461 tggattgctt aacaatttca tcggtagtgg cagcctgctt tttagtttcc tgcttgcagg
     2521 aaacaattac tgctaatccc ataagcagtg caaatatcat tttcaatttt tccatgggta
     2581 gtatagttgt tataattttt actgaattaa aaaaaaactt actgaaaaag agtaaaagct
     2641 gtcagtaaat ttacaataat tttaaaggaa acggaatgga tcaaaataaa aaaaggggct
     2701 cagccccttt ttcaattttt tacttttcgc taaaatattt gtagaatcgg ggtataactt
     2761 caattccttt gaaaaattgt tcaagtggat agttctcgtt tggtgagtgg atggcatcgg
     2821 actcaagccc aaaaccaaga aggattgatt tgattccaag cacttgctca aaagtgctta
     2881 taatgggaat gctgcccccg ctgcggaaag gaaccggttt aatgccgtaa acatcctcaa
     2941 tagccctact ggcagcccta taggctggag tgtttgtggg agctacatac ccctgtccgc
     3001 catggtgtgg ggtaaccttt acttttactg attttggagc aattgactca aaatgctttt
     3061 tgaaaagttg agcaattttc tcgtggttct ggttgggtac cagtcgcatc gatattttag
     3121 cgtaagcctt ggagggaaga acggttttag ctccctcgcc aatatatcct ccccaaatgc
     3181 catttacatc aagcgtgggg cgaattcctg tgcgctccat ggtagtatat ccggcttcgc
     3241 catggatatc atcaatatcc agagctttct tgtaatcatc aagattaaaa ggagcacgag
     3301 ccatatcggc gcgttcttca gggcttacct ccaacacatc gtcgtaaaaa ccgggaatgg
     3361 taacgcgatt attctcgtcg tggagcgagg ctatcatttt ggcaaggatg tttgcggggt
     3421 tggcaacggc tccgccaaag agccccgagt gaaggtcgcg gtttggtccg gtaacctcaa
     3481 cctcaacata cgctaatccc ctaagcccgg ttgtaattga tgggacgtct ttccctatca
     3541 ttgatgtatc ggaaaccaga ataacatcgg cttttagcat ctccttgtta tcctggcacc
     3601 acttaccaag gtttggtgaa ccaatctctt cctcaccctc aatcatgaat ttaacgttac
     3661 agggcagggt gttggtttta accatcagct caaaggcttt agcatgcata aagccttgtc
     3721 ccttatcgtc atcggcacca cgggcatata ttttgccatc acgaatttcg ggttcaaagg
     3781 gttgcgattt ccaaagctct attgggtcaa ccggcattac atcgtaatgg gcataaacca
     3841 aaacggttgg gagttttgga tcgataattt tttcgccgta caccaccgga ttgcccggag
     3901 ttggcattac ctcagccctg tcggctccgg ctttgagtag cgtttcgcgc cagtattctg
     3961 ctcccctaac catgtcgggt ttgtgttccg agattgagct gatggatgga attcggagta
     4021 gcccgaaaag ctcgtcgaga aaacgttgtt tgttctcctc gatgtaggct ttgattgtat
     4081 ccataagatt tgttttagta tttaactatt ctgttcgtaa agataacaat tttgcagcgt
     4141 cctcaagctg gccataaaac tctttgccgt aagcgcggat tatagcatcc ttaaggaacc
     4201 tgtaaacagg caggtctatt tgctcaccca actcacgtgc gggagcgcaa acatcccact
     4261 gatggtagtt gattgcagta aaggttgagt attctttaac cctaattggg taaaggtggc
     4321 acgatatagg cttacggaaa tcaactatac cattgttcca tgctttttca atggcacagg
     4381 ttgctatacc attttcaaat acagtaaaag cacattcctt gccgtcaatg agcggggtaa
     4441 ccaaatcgcc atcgctatcg gtaagggcaa ctccttgctc ctgaacggta gcaataccct
     4501 ggggtgtcat gtagggttta accttatcga ggatttgctc aagggtaaga gcctcgttgg
     4561 gtgttaaggg tgctcctgag tcgccgtgaa cacagcattt ccccaaacaa cgcggcaggt
     4621 tgcagcaaaa ccgttgttcc aggatctcgc tgcttaccac cttgttgtct atttctatca
     4681 tcatttacct aactttttac tggcagagaa ccgttccggt catttcctgg ggtattggca
     4741 ggcccatcat ggtaaggata gttggagcta cgtcggccag aataccatta tctacctttt
     4801 tgaacctgtc ggaaaccagg atgatgggaa cagggttaag tgagtgtgct gtattaggtg
     4861 agccatcggg gtttaaggca taatcggcat tgccatggtc tgcaatgatt accacctcgt
     4921 aaccgttagc ggttgcggca tcaaccacct caccaacaca tgcatcaact gtttgaactg
     4981 ccttttgaat ggcggtgtaa acaccggtgt gccctaccat gtcgccattg gcaaagttaa
     5041 ggcagataaa gtcgtgtgtt ttcttgtgga gttcggtaac aatggcatct tttaccaggg
     5101 gcgcgctcat ctcgggttgt aggtcgtatg tggctacctt gggcgagggg ataagaatgc
     5161 gactttcgcc agggaactct tgctccctac cacccgaaaa gaaaaaggtt acatgggcat
     5221 acttttcggt ttcggcaata cgcagttgct tcaggttttg attgctaacc acctcgccaa
     5281 gggtgttcag taggttatcc ttttcgtaaa taacattaac attcttgaac gtgtcgtcgt
     5341 agcgggtcat ggtaacgtaa tgcagtggca tggtttgcat accaaactcg ggcatatcct
     5401 tttgggtaag cacaatggtt atttcacgga gtcggtcggt gcggaaattg aagcagatta
     5461 ccacatcgcc ttcacggata aggccaacgg gcttaccttc agcatcaacc cgcactatgg
     5521 gcttgatgaa ctcatccgtt acaccctcgg cgtaggactc ttcaattgcc ctaactatat
     5581 cggtggtggg tttaccctta ccatgaacca tcaggtcgta accctccttt acgcgttccc
     5641 aacgcttatc acggtccatg gtgtagtacc taccaaccaa cgatgcaata acgccattac
     5701 ttgattttag gtgatcttca aggcttttca caaagccaag accgctttgt ggatcggtat
     5761 cgcgaccatc ggtaagggca tgtatgtaaa ccttacttaa tccgtattcc ttggtaatgt
     5821 cgcatagctt atacagatgc ttgtccagcg agtgaacgcc accatcggaa accaatccta
     5881 taaagtgaac agccttgtta ttctgcttgg catattgtag cgcattaacc agaactggca
     5941 tttgggccaa tttaccctcc ttgatatctt tattgatgcg aaccaaatcc tggtaaacaa
     6001 ttcgtccggc gcctatgttg aggtgtccca cctcggaatt tcccatttga ccatcgggca
     6061 atccaacatt ctccccgctg gttagtagct gagcgttagg gtatttactg gtgagcctat
     6121 cgatgttagg tgttggagtg ctataaatta catccgattt gtctttgttc ccaattcccc
     6181 atccatcgag aatcattaaa aggactttac ttttaatcat actatagtgt gctgttaaaa
     6241 ggttaaaaac tttctgaaaa ttccttattc aaaaaacgtt tacaagttca agtttgttta
     6301 ctgagtggag cacaaattta ctcttttttg gcgatattga cccgtggtga aaggttaaca
     6361 agtgttgtaa ttccgaaatc gatttctgaa ttcaagtaac taataaacaa atgtttatat
     6421 attgatactt gtttgcgatt tttaatattt agatttaatt tcataacttt ctgtgctaat
     6481 tcattaatga aggatagatg agaaagctga tattcatcgc ttttgcgatt tttgcaacaa
     6541 atgctttttc tcagatcaat actgcagggt tacccatcat aaagcattac cccaaatcgg
     6601 tatatggcgc aggaacccaa aactggtcta tttgccagga tagtcgtgga tttttatatt
     6661 ttgccaacaa caacgggttg ctccgttacg atggcaatag ctgggattta ttcccattgc
     6721 ctaataacca gctagtccga tctgtttact gtagcggcga tacaattttt gtgggggcct
     6781 ttgaggagat aggctacttt gtatataacc gtcaggccga tatggtttat cactcattaa
     6841 tcagcctagt ccctaaaacc gatgtaaatt ttgatgaggc ttggcggata tttaagatcg
     6901 gtccaaaata catttttcaa acttttagcc acattctaga atacagcaaa ggggaaatgc
     6961 gcgtctttag agcccctgaa ctgcttcagt tctcgtttgt gatgcggggc aactactacg
     7021 tgcagggtaa ggagggaaat atttaccgtt accagaatgg tagctttgat agatttaacg
     7081 ataacggtat tttcaacgga aagcaaattt gggcatgctt cgaaatatcg ggcggtaaaa
     7141 ccttgcttgc taccataaac cacggcgttt ggattttcga tggaattacc tttacaccct
     7201 ggaaagggca ggttaacgat ttgcttaaag cgtaccaggt gttttcggca tcgagggtaa
     7261 gtggtggtta catagccttt ggtaccattc agaacgggtt aatcattacc gacgatcagg
     7321 gaaatctggt taagtttatt aataaatcaa ccgggttaca gaataatacc attttaagca
     7381 cttttgttga taatgccaat aatctttggt tgggtttaga taacgggatc gactacattg
     7441 agcttaactc acctctcagc tttattggtg aggggttagg gcttgaaggg gctgtgtatg
     7501 ccacaaaaat ttttgatggg aaaatatttg taggtaccaa ccaggggctt tacgtggcct
     7561 catggcctat tggcgttttt gtagatggca ccaagccttt caggatggtt cctttaacca
     7621 aaggacaggt atggtcgctt tttgagagcg atggcaagct ctactgtggc cataactttg
     7681 gtacttttat tattgataag gggttgaatg ttaggctgat ttcacccgag gagggctcgt
     7741 ggatgttttt acccattcgt agcgttcctg accttatgat tgttggtaag tataagggcc
     7801 ttcatctgta ctcaaaagct aatggtgaat ggaggtacct taaaaagatt gcaggattca
     7861 acgagtcgtc aaggttgttg gttgaggatg ataacgagag tatttggatg gcccacggct
     7921 ataaaggtat ttttaagttg atgcttaacc aaagcaagga tagcattagg ttttcaaccc
     7981 tttacgataa tacaaggggg tttccgggta agtttgccat aaatgttgaa aagattggaa
     8041 atcaggttgt attccttacc tcacaaggtg tgtataggta taactccctt accgataata
     8101 tggagccata ccaggagctg aatcagatta ttggtgatgc tacaggcgtt cggcgtatgg
     8161 ttgaggatga aaaaggaaat atatggatat taaagtcaaa tggtcttcta aagttagtcc
     8221 gcgaagcaga tggagctaaa agggtttatc aaacccccgt tagccgattt ggcgcgaacc
     8281 tcgtagcttc gtttgaaaat atctatgttt acaatcatag taatatattc attggtactg
     8341 aaaatgggtt ggtgtattac aacagccttt ataatcctga actgcaaaaa aacgagttgt
     8401 attgtgctgt aaaaagtatc agctcaactg gtaagcatga aagtgtgatt ttcaacgatt
     8461 atgctgtaac aggcaatgga gggttgtata ttccattcaa ccaaaattca attcgggtta
     8521 cagcggctgc ccctgtttac aatgcaggcg aaattacatt tagctttttg cttaagggct
     8581 acgataacga ttggagtccg tggcagcgtg ataacgctaa ggattttagc caaattccct
     8641 atggtgagta caacctaatg gttcgtgtac gcgatgcaat gggcagaata tctgaaccca
     8701 ctgttgtgca tattcaggtt ggtctgccct ggtactttac ctggtatgct tttattcttt
     8761 acttggtact tttggtagct gccttttata cgcttcgcat tcttgttcgg aggaaggttc
     8821 aacgtgccac tgcaagggta aaagagcaaa agaatttgga gctaaaagta aaggaggagg
     8881 agcataagcg tcaagtgctt gaggcggaac gggagatcat aaggcttaaa acagagaatc
     8941 ttcaggtaca gattgaacac aaaaatcgtg aactagccgc aactgcaatg caaattgcgc
     9001 acaagaacga cttccttaac aggctaaggt tgaggcttga agtgattgct aaatcaatta
     9061 accctgtttc tcagaaagaa gtgctcgacc tcatccgaaa tattgacaac gacttgaaga
     9121 tggacaagga atggcagcgc tttgagcacc atttcgatga ggttcatagc gggtttatta
     9181 agaagcttaa ggagatgtat ccggagctaa cacccagcga acttaggttg tgtgcttacc
     9241 ttcgccttaa catgaccaca aaggatattg cccagatact taatattagc gttcgcgggg
     9301 tggaaataag ccgatacagg ctacggaaaa agctcaaaat tgaaagcgat acaaacctgg
     9361 ttgattttat gctgaacctg taaggctaaa gcttaataaa actgccagtt tgtagcaagt
     9421 ttgtatgtgt aattattctt acgctgtaaa acccatttgg aaggtctgag atattaattt
     9481 gaccatcact attttcaatg atttctttta caagaacacc tgcaatgtta attacttgaa
     9541 tttttacatt tccctttaaa gcgcttgagt tgattttaat ataggttgat gcaggatttg
     9601 ggtaaatgtc aaatctgctt attttattct cacttggtga gaagatggtt agcagtgtat
     9661 tgctcaaaac aacatctgaa atccacaatg aatcagatgc ggctttgccg taaacgccat
     9721 aatttacata aaatcgaaca cctgcaattt gatggtagtt tacttgcttt ccatattcag
     9781 gagtgttcga cccccagttg ttgttaaagt cgaatgagta gtagtggaat acaccatggg
     9841 gttcaacggg gtaaaagcgg aaaacaggcg atacatcggt tagccggcca tcggtgtcga
     9901 aaaggtcggc gcgcattact accgatttgc tttggttgta ggtcatcatc tccacattga
     9961 ggactgtaag gttgctcatg tccacagggt tttgaaatgt ataggtgaaa tacggaaagt
    10021 cttcaggttt agtgatattg tacttaacct tcaaaagcgt atcgacagct gaaagcgtga
    10081 tggtgttggg gctggtattt aaaccagccc aaccaataag tccattttct tttacgttta
    10141 acaggctaaa cctatcgccg gttggaatga ccgctgtttt tactgtcttg gtgtatgttc
    10201 caccagcttg cgggtgtgta atttctacag atacatcacc ctgcacatct ccccagttta
    10261 caacaattga gttagttccc tgaccgctaa caatggttgc acctgcaggg acactccagc
    10321 tgaacgttgc atcggcaaca ttctgaatgc tgaatgtaac gcccgtttgg tttggagaaa
    10381 cgtattcggg acccgaaacc ttcaaggccg atacatcgat gtattggtaa acccgaacat
    10441 aatcaacata cattttttgt ggccaaatgt tggggtcaac tccttgagcg ccaccccagt
    10501 tgccgcctac ggcaatgttt agaataagat ggaaaacctg atcgaatggc caggttttgt
    10561 aatccttgtg ctcattatcg aaggtgaagt acttattatt atcaacatag atgtcgattt
    10621 tttcgggggt ccattcaaga gcgtaaagat ggaaagcggt ttcggcatcg ggtaccgaga
    10681 ttgttgcggt tttttgagtg ccaagaacat ggttgtaggc ttcggtgtga atgcttccgt
    10741 gcactacccc ggggtcgtag ccaacatatt ccatgatatc aatttcacca ctcttaggcc
    10801 aactgccata aacccagttg gtgggtagca tccaaatggc aggccatgtc ccagtacccg
    10861 aaggcagttt ggcgtaagtt tcaaccctac cgtaaagcca atcgccttta tagcgggtaa
    10921 ctaatcgggc cgaggtgtac tcgctgccct cgtactgttc ctttctggcc tcgataacta
    10981 ggttcccatt ctcaacgcga gcattttcag ttcgtgcatt agtgtagtac tgtagctcat
    11041 tgtttcccca acctcctcca cctacatcgt aaccccattt atcagtggca ggcaatcctg
    11101 tgtagttaaa ctcatcgttc cataccagga ccttaacctg agcaaggctt gtaaagggaa
    11161 ctactagtag tagtgctatg atgctattga taaactttct gctcattctt tttttttgtt
    11221 tttaagaaaa aggctttcgg gaattgtcca tctcacctaa ccatacttat acgaaagaaa
    11281 actttttttg gacaattaat ccgaaagcct tttacccaaa gttacttcaa cctaaagctc
    11341 aattcttgca agtcgcggct gttgccacct acaaacacct tgaaatcgcc gggttcagct
    11401 ttaaattcca tggtgcgtgt ataaaaagcc aggtcgcttg cggtaagttt aaacattacc
    11461 tttacggttt ctcctttttt gatgaacacc ttctcaaaac cctttagctc cttaactggt
    11521 cgggtaacag atcccaccat gtcgcgaatg tagagctgta caacctcttc accatcgtaa
    11581 tttccggtat tggtcacatc tacccaaact ttaagggtat cggtcatgcc aatttcattg
    11641 gtgctaacac caaaattact gtaatcgaaa gaggtgtagc taagtccgta gccaaatggg
    11701 taaagcggtg tatttggact gtcaatatac tttgaggtgt acctttcggt ttctaccctt
    11761 ggtctgccgg tgtttttatg gtaatagaat aatggaattt gtccaacatt acgggggaag
    11821 gttattggca gtttgcccga tgggttgtag tcgccaaaaa gtacatcggc aacggcagga
    11881 cctcccattg tgcccgggaa ccatgcttcc agaatagcag gaactgcatt atccatccac
    11941 tcaatggtta acggacgacc attcatgaga acaaccacca tgggtttttt aagttcattg
    12001 attgccaaaa ccagatcctt ttgaactcca ggtaaatcga ggaaagccct gctgagcgcc
    12061 tcgcccgaca tcatggccgc ctcgcctact accaaaacaa cagcatcggc tttgcgagct
    12121 gtggctattg cttcggtaag tccagctttt gaatcgcctg taatatcgca accctttgcg
    12181 tgaattacct ttgtgttggg taatttttga cgaataccgg tcaaaaccga tacagcatcc
    12241 ttgccttcac cccttgctga ccaggttcca atgatatcct cggtattatc ggctaatggg
    12301 ccaataactg caacggtgtt aagttttttg ctaagtggca acaccccatc gttttttagc
    12361 agaaccatcg atttacgtgc aacctcacgt gcaatctcca ggtggcgagg gttaagtatt
    12421 aactcttttt cccggattgt atcgcagtag cggtaagggt catcaaacag gccaagcatg
    12481 aatttaatgc gaagaatacg ccttacggca tcatcaatct gcttttcggt aattttgcca
    12541 tcggcaagta acttcttaag ctgatgtatg tagatatgac cctccatatc catgtctatt
    12601 ccggcattca tggctaggtc ggctgcttgg tagtcgtcag cggcaatccc atgaaccagc
    12661 atctcgcgta ttgaacccca atccgaaaca ataaatccat taaactgcca ttcgtccttt
    12721 aggatatcct taaccatttc cttgttacca gtaacgggta ccccgtttag ctcgttgaac
    12781 gacgacataa atgttccaac accagcatca atggcagcct tgaatggggg taggtaaact
    12841 tcgtgcagtg ttctgtttga aatatctacc gtattgtaat cgcggcctcc cattgctgct
    12901 ccataggcta caaaatgttt ggcgcaagca acaatagttg atgggtcagc caacgatgag
    12961 ccctgaaaac cttttacccg tgctttcgcc acaagcgaac cgtagaaagg atcctctccg
    13021 gctccttcca ttacacgccc ccagcgtggg tcacgggcaa tatctaccat tggtgcaaaa
    13081 gtccagtgct gccctgcagc agcagcctct atggctgcaa tacgtgccga tttttcgatg
    13141 gcatctaaat cccaacaggc tgcctcgccc agcggaatgg ggaatattgt tttatagcca
    13201 tgtattacat cgtagccaaa aatgagcggg atacccaaac ggctttcctc tacagcaatt
    13261 ttttgcacct tataggtgta ggctgcgcca ttaacgttga gcatgcttcc cacttcacct
    13321 ttccgaatag catcctggta ttcagtggca aaggtgatag ggccagtggt ctctccatta
    13381 ccggttagct ggtttagctg gcctatcttc tcgtcaatgg tcataagctt tagaaccgag
    13441 tcaactttcc gttcaattgc tggatcgata gatagtttgc cccccgaatg gtccattgtg
    13501 gctttattgc cactacacga ccatgctgcc aacgttacaa ctgcaattat taatttcaat
    13561 ctcatcattt tccgtttttc gggtttaaac agggtaaccc gttaagggtt accctgtgaa
    13621 cagaatagtt tacttaagcc cttcgatgtg aggcccagct aaatcgtcga ggtgaacgct
    13681 aaaggttcct ccgttgttac cggggttgat cattatgcga accacgttgt agaagtcctt
    13741 gttaaacctt gagtcagtgg ttacatcgcc ggtgaaaatg tcacctgtcc aatcccaacc
    13801 tgcaccaact ccggcaaagt taaactcggc aatttcccat ttattatcat tctggatggt
    13861 gtagatcaaa tcatgggttc cagtttgcca agcattaccg cctcggtcag tattttcgag
    13921 tttcaaaagt acctggtcgc cagccttacc ataaaccaaa accttaaata cggtttgttt
    13981 ggtaaggtca aagcggtagc catcgggtag gcgagtaaat gcgtttgccc actgggaatt
    14041 gtattttgtg aatagagcgc aatgtgcact acggttcggg tagagggtat tgttggggtt
    14101 ggttactatt tcaagtgtgg ctcccccatc ggactgatca acctgcatgg gaacaagggt
    14161 ggtttcgcca aagtcgttgt acattaccat agcatcgagc aggtactcct ggtaggttgg
    14221 gtgattagcg gcaacggcaa ccgttgaggt tttaacaaaa tcctgcccgt tagctgttac
    14281 tgtaaccttg gtgttgtaat ttcccttacg catgtaggtg tgagttacca cctcggctcc
    14341 attggtggtt tggtagctgg agccatcgcc aaagtcgtaa gtaatattcg aaatggtaaa
    14401 tgatgagggt actgttaaat cctcaagagc aaatgtgtac tcgttggggt tagcggttgc
    14461 tgtaacattt agtgtatatg atatagtagg tcttacatac cctttaggaa ttatcttatg
    14521 gtaacgggca gcaccaccaa cagaacgtaa gtggaagaag ttttcgttcc attcaagaac
    14581 ttcgtatttg cgggggttgc catcgtcgta agagatattg cctgactgac tcgagatctg
    14641 tatatagtat ttaccgttct cctcgctgat tgaccatgta agttcaccac gggcggcatc
    14701 gttggtattc atgtcgatgt cgtaggcgtc gttccagaac gatgatacat aataaccagc
    14761 ggataagcca tcctcatcgg cattaccatc gtggtttaca tgggttttgc catgagtgtc
    14821 aatggtatag gtaaatccca caagcttaaa ggtaaattca tcgtcgtaga ggtagtgacc
    14881 cgatttagca aggggagcag cagcccacca tactggtgaa tatgaatcgg cagggccaac
    14941 accaagatgt gcttcggaaa ggctatcgat tacccatgtt ttcccattag gtgcatcaac
    15001 tccacctgtt agggcagtaa tgataggact ttcaaggtaa gaccaatccg tttcagtttg
    15061 ttcaaactct tttgtttttg aggtactacc acccgaggca tatagggtta gagtgatatt
    15121 gtaggtttta gcaagagggt agtatgcagt aactgaattc ccaacggctt taataccgtt
    15181 acccaaatcc cagtttgcaa taccttttac tgtagatgtg ttagtaatta caaagtgaaa
    15241 tgcatcatcg ccaggggtaa tggtaaaatc tagctgctca gctgtagggg cattcccaat
    15301 atttggttta tctagttcca tgggctcgca accaaaccag gtaagcccaa caagaaccag
    15361 agcggaaaat atgatgtgtc gtttattcat agctcttctt aatttattag taaccagcat
    15421 tttgttttag tttattcccc gaaagtgtaa tttctgtctg gggtattggc aacaagccct
    15481 tagttgccga gctgaatgtt tcatccagag gacttgcacc tgctgctgcg ttaatctttg
    15541 tttcagcata ggttaaaccc ctacggagca aatcccagta acggagccct tcgccagcaa
    15601 attcaaccct gcgttcatgg taaatgaggt caagaccagc ctgatcgttt gttagagtag
    15661 gtgcaacgtg tgcatcgcca aaagcccgtt tacgaacacg gctatagtag tcctgtgctt
    15721 taccaagatt gttgtcgagg tatggctcag cacccataag cagtacatcg gcaaagcgga
    15781 ttacgggacg gttataaggc cagttcaagg catcgttagt ggcgggcctg tcagccttac
    15841 gagggtagaa ctttttgaat gcatacccgg tatgctggta gcagttttca ggcttgtaaa
    15901 ccaaactttc ggctgcagca tcaagtattg atactgagta acgcgaatca cctggttcga
    15961 actcatcagc aagtgattgg ctggcaggct gaaaactcca accggactcg taaatgggat
    16021 cttcaacacc gcggaaacca gtcattacaa cagcccagtt gccttcggta ccgttacgcc
    16081 atccccagtc gccccagtca gccttttggg tgtgctgaat ctcaaaaatc gattcattgt
    16141 tattctcgtt atctacaccc caaaggttag catagtttgc tactagtcca tgtccgctat
    16201 tctcaacaat atcatcaata atggtttcaa tgtctgtttc tgtaaaggtt ttatcatcgg
    16261 cttttaggtc ggttgtgata tttagaacac ctttaccata acctttatgg aacaggtaaa
    16321 cccttgccag caatgcaccc gcagcccact ttgatgctct gcctttttcg gcagcagtaa
    16381 tagtagcagg taggttttgg taagcataat acaaatcgct ggcaatttga ttgtaaacat
    16441 cagcaggatc ggcagcagat tgttgatact ccgatggtgc aaggggctta aggattagag
    16501 gaacatttcc gtagaactga acaagttcaa aatagaaata accccttaga aaacgagcct
    16561 cagcagtaag gcgggttttt aaaccctcgg ttttgaaatc aacaccgtct acctttgaaa
    16621 ggaacagatt tgctctgtaa atgcctgaaa atctgtcttt ccaaatgcct agaggttcct
    16681 cgtcagtaac aaaatttgag agtttaccca tacgaactaa gccaggacgg tcgcctgggc
    16741 ctgatccacc tgcaaaagca tcgtccgaaa gaatattact tacaagatcc catgggtggt
    16801 agccaccacc tgtttgccat tgcaaaacat cgtagattga aacaactgct tcaaaggcgt
    16861 ctttctcggt tttatagaag ttatcctcaa ccctgtcagt tagaggttgt aactccagaa
    16921 aatcctttgt acaggcaaca attccaagtg ccatgaaaag gattattgat atatttttaa
    16981 tttttctcat actcattgta ccggtttagg ttagaaactc aggtttactc caaacatgaa
    17041 agttcttggc tgtgggtaga tatttcggtc gattccaacg tcaagagccc actttgcgcc
    17101 aatttcggga tcaaaacctg aatacttggt gaatgttaat aggttaaccg cagtggcata
    17161 aatacgcaac tttgagattt ttaccttttg gctgaacttt tgaggaagtg tgtagcccag
    17221 ggttatgttc ttcaggcgga ggaacgaacc gtcctcaata tagaaatcgg aaactctgaa
    17281 gtagttttgg ttggggtcat taatggttac gcgtgggtgt gaattgcttg tgccttcgcc
    17341 ggtccagcgt tcaagaactg aggcatccca gttcgacatg ggcaggtcgt aacggcgtgt
    17401 tcctttgaaa atctcctgtc caaatgcacc ataccagaac atattaaaat cgaaatcctt
    17461 gtaggttaac cctaaattga taccaagcgt gaagtcaggg taggggttac ctatcatggt
    17521 acggtcttta tcgtcaatag taccatcatc gtttttatca acccaaatta ggtcgccagg
    17581 tttagcgttg ggctgaatta ctgtaccatc cgatgactta tagttttgaa tttgttgctc
    17641 gttttggaat actcccgcag ttttataacc ccagaaatag gcaattgggt atccagtttc
    17701 cattacgcaa atattataca tacctacagc aagtcctgca ccataaattt taccctcgct
    17761 gttgttaatg gaaagcactt cgtttttatt gtaagcaccc gataggttag cattaattcc
    17821 taaatcattg attagggttt tgtaacccag ttcaaactct atacccttgt tacttactga
    17881 accaccattt actgtgggtg cagcgttacc aatatatccg ggtattgggg ctacaaccaa
    17941 caggtcttta gttttcttgt tgtaaaggtc gagagtaaaa gttagcttat cggaaaggaa
    18001 acgggcatca acacccacgt tggtttgctc tgaggtctcc cattttaggt caggattcga
    18061 tatacctaca ggatttgaac caagggtgat tacctcatcg gtaccaaaag tgtacttgct
    18121 gcctgaacct attagcgaag tgtacttgaa agcaccaata gcttcgttac cattacggcc
    18181 ccagccaaag cgtagcttta ggaacgataa aggtgtaaaa ccttgcagaa attcttcctt
    18241 ggacataacc cagccagctg agaatgcagg gaagtatcca aatcggttat ttgaaccaaa
    18301 gtttgaggaa ccgtcggccc ttaatactcc ctcgaataca tatttaccgt caaagtcgta
    18361 gtttacacgg ccaaagtatg aaagcagggc gtgctcatcg gcaccacccc atgagcggta
    18421 gctgtcttcg tcggtaccgt tattaatcca agcatgctca aaatcattaa aaattaggtc
    18481 gtttttctta cctccaatat actcatggta gtattatttg ctgtcatacc taccatggca
    18541 gcaaagttgt gttggccaat ggtgttgttg taggtaagag tgttctcact ctgccaggtg
    18601 aaccatttat taacagtacc ggaggcttcc gatgtagtgg cttttacaat atcgctaagg
    18661 aagtataccg gaacaaatgt acggcttgaa ccgttagcgt aatcaatgct aaaattggtc
    18721 tttgccttta atcccttaat aatttccagt tcaccccaaa cgtttcctac cagtttgttt
    18781 tcagagtagt ttgagttaag aacgctaagg taagcaacgg ggttaacaac ttcctgtgtg
    18841 gcatagtttg aaatgcccca tgttccatcg gggtttttta ctggggttat gggatccata
    18901 tttacagcct tagctagcgg gccaccaaat tcggcgttgg ggtcaatacc acgagttacc
    18961 ttattggtat atgccaggtt gttgcccatg ctgaacttgc caatcttatg ggttgagttt
    19021 agacgatagg tgtagcgttc gtagttcgat ttacccttgg caactatacc atcctggtaa
    19081 gtgtaggaga gcgatgaaag gaaggttgaa ttttcgtttc caccgctaac cgagaactgg
    19141 tgattgtatg ttggagcatt cttgtaaaaa agttcatcct gccaatcggt gccattacct
    19201 acagcctgaa caactgcatt aacatcgggg aaaggaatgg ggcggttgtc gtttgcgtag
    19261 ctctcattca tgataattgc atactccttg gcattaagta gcgacacctt acgccatgcg
    19321 ttctggtagc ccatggagta atcgtagtta acggagaact tgcctttctg accctgtttg
    19381 gttgtgataa gaacaacacc gtttgcacca cgtgcaccat aaatagccga tgctgacgca
    19441 tccttaagta tttcaaccga ttggatatcg tttgggttta agtagtcaat tccaccaact
    19501 ggcataccat ctacaatata taaggggtcg gatgtgccgt tggtgcctac accacggatg
    19561 cgaaccgtga acccttcacc cggttgccct gagttggcta ttacctgtac accagcagtt
    19621 ctaccctgaa gaacttgctc aacacgtgta ggtgcagcct tctcaatatc ctcgctcgat
    19681 acttttgata ccgacgacga cactaagctt ttcttttgta caccgtaacc aaccacaatg
    19741 agctcctcaa tcatttgggt tgattcctga agtacaacat caatttgagt tctaccgcct
    19801 acgggaacag tttgggtttg cattcccaca aacgagaact ctaacgtttg gtttggcagt
    19861 acacttatgg tgtacttacc atttgcgtct gttatagcac cctttgtagg gttttccttt
    19921 actacgatgg taacccctgg tagagttgcg ccatcgctcg atgtgacagt gccgctcacg
    19981 ttaatttcct gtgcgagcag gctaccgcac caaaggatta aactaataac tccgaagatt
    20041 tttttcatac tcaattaggt ttagaaattg gttttcggtg tgcaagctaa attcttttaa
    20101 aagccatttc aacttttttg gggcttcatg gattgcacaa cccaatttca acactttctc
    20161 atcattcgaa aacgttttcg ataagctaca acaaaagtac atcaaaatat tgccttgaat
    20221 gtgttaatat attcacatac agtgtgttaa ataaattact tattttgatt tttgagtaca
    20281 attttgaaac aatttgaagg aaatttgcat aatagttgta taagggttga tgtggtatta
    20341 attttggcat aagaatcgag ggggtggaat gaaaagattt tcaggtactg tcttggtagc
    20401 ttttctggtt gctgctgcag ttaaggatgt tggcgcacag gccccatccg ctgatagcct
    20461 tatcttatac cctacaatca ttctcgatgg cgagtatgta ccccatatca agattgaaga
    20521 tgttgttaag gtaggtaaac gccgtttcaa gagccgaaga gaaatgagcc agtactacag
    20581 aatgatatac aaccttaaga aaacatatcc ctacgctcaa attgcaaagt ataagttact
    20641 agaaataaat gagaatttga aaacccttaa aactgaccga gagaaaaagg aatacattga
    20701 caaggccgaa aaggaactcc gaaatcagtt tgagaaggat ttgacaaagc ttaccatatc
    20761 gcaggggaaa atgttaatta aactcatcga tagggaaaca gggcgtactt catatgagct
    20821 tgtaaaggag ttaaagggag gtttttctgc cacgttctgg cagggcattg cccgcctttt
    20881 tgggtcgaat cttaaaacca agttcgaccc gcagggcgag gataagattc tcaatgagct
    20941 aatcttcctt tacgagcagg ggcttatatg aaagtttact ttttgatcag gaaggagtag
    21001 tcaacattgt caacatcaac atactttccg ttaatggcaa ttgaatcgag tgggtaggct
    21061 gtgcaacagc catcgctact ctttacgtat cgtaccagta tggtatcggt atcgtattgg
    21121 ttaaggtata ggtaaaacgt gttgagctgc tcaaagggtt gtagaatacc aatattagtt
    21181 tgtattatat cgttttggcc aactggtcca acatagtaaa tgttggcgta tttttttgtt
    21241 acaccatcaa aatagtagat tttaatggta tccctgtggt aaaacccttc agctattaaa
    21301 ttttggagcg ttgttttatc aacaacctta agccatagtg gcgatggtgg attaacacaa
    21361 tcaacatcgc atcggttaag gcagctgtag ctaaacattg tagctataat ggttgccagg
    21421 agagcaagtt ttttcatggc taaatccatt tgagtatttc tgaaacaaca gccttggctc
    21481 cattcgatat gtcaacaata ttggcatcca gcatatagca aggtgtagta aacagcatgt
    21541 ttttctcatc cacaacaacc tgtgcgtggc cggttggttg gtgcttggcg cccaacgatt
    21601 caattgcttg agctgttgat gtatctttcc caatggttag ggtggcattc ccaataattt
    21661 ttgcaagtag cacgggtgct atgcacagcg ctccaatggg ttttttaagc ttatgcatcc
    21721 cttttatggc tgcttccacc tgagggtgta tgctgcattt aggcccatca aaggcccagg
    21781 tggaaaggtt tttggcaacc ccaaaaccac caggaatgat gagggcatta aaaagttcag
    21841 ggttaaactc atttaacggt tttatcttgc cgcgggcaat ccgggccgat tcaaccagca
    21901 cgtttctttt ttcgttcatc acgcttccgt ctatatggtt tatcacgtga tactggttta
    21961 tgtttggtgc aaaaatttcg taagttgcac catgttttgc tatggcgtaa agggtcatga
    22021 ctgcctcatg gatttcagag ccatcaaaaa cgccactacc tgaaagtatt acagcaaatt
    22081 ttttattgtt tcccattagt gcaaacgttt taaatttgct aaacaagata gtttgttttt
    22141 tatttatacg caatacctaa atgggaacat catggaaact aaacgtactt ttttggcaat
    22201 tgatgttaat cctcaggata atatcattaa cctgcttcaa gagttgaagc gcaatcataa
    22261 actcgatagc atcaaatggg ttgacccggc aataatgcat cttaccattt tctttttagg
    22321 cgatacccct tccgctcaaa tcccaagtat atgtgaaatg ttaaaagcaa ctataaaaaa
    22381 atttgaaccg tttagcataa tccttaaagg tataggttat tttgggcgcc catcgccaag
    22441 ggttatttgg gtgggagttg atttccccaa gttgcttaat gaacttaaac tttcagttga
    22501 taaagttttg agcgagtttg actttagcga tgataaggaa aaattcaatc cgcatcttac
    22561 cctgggcaga gtaaaattta tgcacaattc cgataagttg aatgcagcaa ttcagcaata
    22621 ccgaaataaa tcattccagg tacaagaggt taaggaactg gtgctctacg aaagcatttt
    22681 aaaaccgcaa gggccagagt atagggctat ccagaaattt gagctaaagg gatgctaaac
    22741 tcccttcccc ttaaaaatgg ttagtatggt gtatatcatt atcttaaaat cgatgtaaag
    22801 cgacatattc tctaggtata aaaggtcgta cttgagccgc tctaccattt gttcgatatt
    22861 ttcggcatag ccatacttta cttgtcccca cgatgtaata ccaggcttta ccttttgtag
    22921 atgaaggtaa tggggggcaa cttttactag ttggtctata tagaattgcc tttcggggcg
    22981 tggcccaaca attgacatct cgcctttaag tacgttgatg aaatttggaa tctcatcgag
    23041 ccgggttttg cgcataaatc gacctacagg agttactctt gggtcggttt ggtttgatag
    23101 agcgggtccg tttgcctcgg catccacata catactcctg aacttgtata tatgaaaagg
    23161 tttaccatgc tgtccaatcc tttcctgttt gaaaataaca gggccttttg atgttgtttt
    23221 aattacagcc atgagtataa gcgcaactgg ggctgagagc aaaagaccaa caatggctat
    23281 aaagtagtcg agcatttcct ttaggtttgc ctgccaggca ggcatgggct caaacgagac
    23341 atctaccagg gtagtcccaa taatacgatt cagctttact ttccccagca aaatatcgta
    23401 catgctaggt attgctttga tgtttacatt tagcggaaca agtaggttta aaacggttgt
    23461 tagcgtttgt gggctttcat tgtcaatggc aagaataacc tcttcaattc cgtatcttgc
    23521 tacaacttcc ggtatgttgt ttacacttcc caaaataagc gattggtcaa ccctgacgcc
    23581 attgggtaaa tcgataaaac caattattct gtatccttgc gatatttttt ccgagttaat
    23641 atcgttatag gtttgcatgg cttttttccc actgcctact attatggttg ggtagccaat
    23701 ttttctgtta tggatccggt gaatggtttg ggttgtaatt acaagtcggg gtgtataggt
    23761 gagcgcaaat acaagcagaa acatggccaa caaaatcttg tagtagttct ggtaagctac
    23821 cacgtaatcg tcaatcagca gaacaaagaa aagcacaatg gttccaataa tggtaatagt
    23881 tagcgaggct ccaaattctc tgagcctcga ctttcggtat gggtcgtggt aaagcccaac
    23941 caagccaaac atcgaaaccc aaaataatgt gaagaataca agccctaggt aaaacttata
    24001 gtcagggttg aaacgaatgt caatgccaaa aaggtttgat tcaattaccc ttttacggta
    24061 tagattgaat aacgtccatg ccagggcgct accagctaca tcgaaaagca aataccggaa
    24121 tgcctgcttt tttttgttca aagtcgcaac ttttattcaa tataactacg aaacatttaa
    24181 aatattgcta gctgtagatc ttttttatga ttatttctat gatttgcttc gattttaggg
    24241 ccatggcagc cgggtacaac atctttcgtt ggtttatgaa gtaatccacg ttttgcataa
    24301 ccgtttcttt tgttgattct ggttttattt gggcttcaaa gatattgaaa gttttatcat
    24361 actcctcggt tgttagcaca acctttaaca tgctcgtttc tttcttgttt ttaatttgat
    24421 aggtaatcaa agagttgtcg ttgaaatcaa ctatcagttt cagggttgaa actggatggc
    24481 tgtataaggg caaccagtaa accctaacct tgcggagttc cgattttatt ggtgataaaa
    24541 ggaatattaa gtcatttgca atttgttgca ataaattttc ggttgaggtt aggttgattg
    24601 tctcttttac gctatcaatt atgattgggc tgtgggttga tggaagttca aggctatatt
    24661 ttggtaaaaa gccaaactca accccaatct cttgggctat ggtaataagt tcctgaattt
    24721 tttcgctgct gaaatgggga agcgatggcg ttaatacgtt aatgccggct cgtagtgcat
    24781 tggcaattat tgtgtacgag gtttttgggt tagtgtcaac taccagcaat tcagggtagt
    24841 taaacagcct tgctatttgt gagttgcttt cacatatccc aagcaaaatc ttatcaatca
    24901 gttggttgtt ggcttcaatg gtgttattga aaaaattctc cccctgatac tgaattaact
    24961 tattgctatc ggttacaagg caggttgtca tagttaaagg ctaatgtttt gcaataatac
    25021 attgttctgt aatcatttca gtggctcact cctcataatt tattaacctt ttagtaacag
    25081 ttggaatttt atgctttata aaaaactaaa tttgcttgcc atttaaatga tattaaaaaa
    25141 tatgctccag gacgatagta tacgtcacaa aggtttacgt cgcaagctag tggaagaggt
    25201 tgctgccaag gggataagcg ataagcgagt actcgaagca atgcttaagg tgcctcgtca
    25261 ttttttcatg gatagtggtt ttttaagctt tgcctacaag gaccaagcat tcccaattgg
    25321 ttccgggcaa accatttcgc agccatttac tgtagccttt caaacgcagt tgctacaggt
    25381 tagtcccatg caaaaagttt tggaggttgg aactggctcg gggtatcagg ctgccattct
    25441 tatggaaatg ggtgctaggc tctataccat tgaacgccac cgggaattgt ttcttgcggc
    25501 aaaggctcta cttgaggaga tgggctatcg gcctagctgc cattacggcg atggctacca
    25561 gggattacct ccatatagtc cattcgatcg tataatagta acggctggcg ctacagaagt
    25621 ccctcaggct cttgtcgatc agctcgcacc aggggggcgc ttggtaattc cggtagggaa
    25681 tgattatggc caaaccatga tgctcattga gaaattagat gatggaacca taaaagaatc
    25741 ggctcacgga aactttattt ttgttccgtt gttaaaaggt aaagcaaaat agtaagctaa
    25801 tgattgcaat taaacagttt gttttcaatc ctttccagga gaacacctat gttctgtacc
    25861 aaccacaggg cgaggccatt gtggttgatg caggctgctc aaccgaaagt gagtttcagg
    25921 aactcttgga ttttgttcat agccaaaacc ttacggtaaa atgtttgata aacactcact
    25981 gccatatcga ccacatacta ggaattgata agctaaagaa gcaatttggc gttaaggcct
    26041 ttgcccatat cgatgatttt cctttgcttc aaatggcccc agcacatgcc atgatgtttt
    26101 ggttatacct tgaaacagta ccagcaatcg atgaaacact cagccatgga gatatgatag
    26161 aacttaacgg tgatagtata aaggttattc acactcccgg acactccaga ggcggtttgt
    26221 gcttttatgc ggagcaggaa agatttattt taaccggtga caccctattt aacctatcca
    26281 ttggccgtac cgatttggct ggtggtaatt acgaaacgct gattaagagc attagggaac
    26341 agctcatggt gttacctgac gatgtgatag tttaccctgg ccatggtaat agcacaacca
    26401 ttggaatcga aagggcatcg aatccctttt tgtagtttag gtgttaaaaa acataagcaa
    26461 aaaacttttg tttagttaaa atcaataacc caaattcata attttgctga taatctttaa
    26521 ataaaaaacc taaaacatgt ataccgataa atttgtgtca cgtcataatg gtcctagcga
    26581 ccatgaagtg ggtgaaatgc taaaggtaat tggcgtttca tcggttgatg agctaatcaa
    26641 ccaaaccatt ccggccaata tcagacttaa aaaaccgcta aacctacctg ctcccatgtc
    26701 ggaatatgag ttcctgaccc atatgcataa ggttgcatcc aaaaataaac tctacaagag
    26761 ttttattggg atgggttact atggaaccgt taccccttcg gttattttgc gtaacatatt
    26821 tgaaaacccc ggttggtaca cttcgtacac tccataccag gccgagatta gccagggaag
    26881 gcttgaggca ttgctcaatt tccaaaccat ggtggttgag cttactggaa tgcaaattgc
    26941 taatgcttca ttacttgatg aagcaaccgc tgctgctgag gcaatgctca tgatgctcaa
    27001 cctccgctca cgcgaggctg ttaaggcagg caagaatgta atttttgttg atcaggatat
    27061 tttcccacaa tccctcgatg taattaaaac tcgttcaaat ccgcaaggga ttgaagttgt
    27121 tacagggtgt tatagcgagt ataattttac tggacgagaa tttggcgtaa tagttcagta
    27181 cccatcggca aagggaaaca ttcgcgacta tgccgctttt gctgcaaaag cacacgaggc
    27241 tggtgcactg gtaacagccg tttccgatat tttaagcctg gctctaatta cacctccggg
    27301 cgagtggggg gccgatattg cttgtggttc tactcaacgt tttggaatac ccatgggatt
    27361 tggtggccca catgcagcat actttgcctg caaggatgag ttcaagcgta gcatgccagg
    27421 ccgaataata ggtgtttcca ttgacaggca tggcaacaaa gctctacgta tggcactgca
    27481 aacccgcgag caacacatta agcgtgaacg tgccacatca aatatctgta ccgcacaagc
    27541 cctcttggct accatggcag gtatgtatgc tgtttaccac ggccccgaag gaatacgccg
    27601 tattgcctcg tatgttcaca gcactgctgt tcaggttgcc aaggaacttg ttggtatggg
    27661 ttacaagcaa aacgttaaga atatttttga cacccttgag gtagaattgc cttcaggtgt
    27721 aaccatcgat gatatccgca agcgcgctct agctcgcgaa atcaacctga attacaagcc
    27781 aaacggaacc gtaggtatta gctttgacga aaccacttcc gtaaccgatg tcaacaacct
    27841 tctttcagtt tttgccgaat ctgctggaaa gaagttcaat tctatagccg agattaagga
    27901 aaccattgaa atccctgaaa accttcgtcg caaaacgcct ttcttaaccg ataaggtatt
    27961 caacagtttc cattccgaaa cagaaatgat gcgctacatc aagaaacttg agcgtcgcga
    28021 tatagcccta aaccatagca tgatttcgct gggtagctgc accatgaagc taaacccagc
    28081 aaccaccatg ttgccgctaa gttggccgga atggaatagc ctacacccgt ttgtccctgc
    28141 tgaccaggcc gaaggctaca tgcaggtcat ccgtgaactg gaacaatatc tggcagagat
    28201 taccgggttc catgctgtga ccttacagcc aaactccggc gcagctggtg agtatacagg
    28261 gttaatggta atccgccagt accacatcaa tcgtggcgag ggccacagga atgttgtact
    28321 gattccttcg tcggcacatg gtactaaccc cgcaagtgct gctatggcag gcatgcaggt
    28381 ggtggttgtt gcttgcgatg agaaggggaa tatcaacgtt gacgatttgc gcgctaaagc
    28441 agagcagcac aagcagaacc tttcagcatt tatggttacc tatccgtcaa cccatggagt
    28501 atttgaaagc aagattaggg agatgatgga tattgttcac cagaatggtg gtttggtata
    28561 catggatggt gccaatatga atgcccaggt tgggcttacc agtcccggtg agattggtgc
    28621 ggatgtatgc catcttaacc tg
//
LOCUS       DAOOWJ010000025        28067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1699_length_28067_cov_7.935349, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000025 DAOOWJ010000000
VERSION     DAOOWJ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 28067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28067
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1699_length_28067_cov_7.935349"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLW22_04145"
     CDS             <1..545
                     /locus_tag="PLW22_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HOU73929.1"
                     /translation="DGYHPKDDTSCVTIYFRNPRKLLICTGPPYEKENDTKLAKIFDT
                     FEGKKAICGATTVDIIARELKRSVEDSLEFTDPDLPPISQMEGADLVTEGILTLSKVN
                     TILESYNENTNLGKGPADELVKLILESDSIEFVIGTCINVAHQDPNLPVELEIRRTVV
                     KRIANILQDKFLKEVKLQFL"
     gene            679..2958
                     /locus_tag="PLW22_04150"
     CDS             679..2958
                     /locus_tag="PLW22_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU73930.1"
                     /translation="MRSFRFFLLNMLIATYFSAVSYGQDWNSIFTLETDAEYYMAEGN
                     FSKAADTYVKALKKFPESANLKFKIGYCLLKTPDRKSEAISYLEEAVMKISDKYDAKS
                     LKEPNAPPEALYQLGMSYMYQNDFDNALKSFREYQGYIKPKDEHAKLMAEHAIASCEN
                     AKKFQKSPIGIKYNRLGPDFNNENDNFNAVVSGDGSTLAYTTRTSSGNKIFVAKKGED
                     GLWAKPVDITRNLGSKNLTTSYLSYEGTELYLIENDPRNSEIVVSFLQKRKWSSPVKA
                     AKPINSKFNETHVCVTRDGNTVYFTSDRKGGQGGYDIWASTINNGRWSDPINLGPNVN
                     TPFDEATPFLSPDEKYLFFSSQGHNTMGGFDVFFTNLQGSPNVKNAGYPLNTTDDEIF
                     YFPTGLSSGYIAKFSPDGFGKLDIAEVEIIPMVDVKLNLMLADNAPLDKYYNVNVTNN
                     STGEIVEKQSVKGKTQLSLKVKPGDYTVTAQGEGFEETKTPLSIPAKPDKSEYQASLM
                     LNPRVVQQPVAQLEPPKEEPKPEVKPEVKPEPKPEVKPEARATDKPKAVEPEPKKEPV
                     VKQAPKKEPKPEVEKPKPEPKPAKALPPTPPPTAAPTPRMDFNKTSSTRTLAKPTYSV
                     QLMASLYPVDYNYFKLDSISVTISPDGYYRYSVGNTDNIKEIEELQQKVRGHGYSNVW
                     VRINREHPGYTIQFLALQKPKSIKQFTELSDVMVYRGNDGVYRYCVGKYSTPEEASSD
                     LAKITGLGYSGAFVRELGK"
     gene            complement(3011..5245)
                     /locus_tag="PLW22_04155"
     CDS             complement(3011..5245)
                     /locus_tag="PLW22_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU73931.1"
                     /translation="MRLIKLFIITVFVLGNNVLAQNIIRGIVYNENKIPLEGANVFIP
                     MLVKGTSTNSSGEFELTGLPNGKFDIQISHIGYASQIVPVTLGKTQFELDVVLIPASV
                     EAERVVVSTGYSTTQHQNAVKIEVLRLNEHHLIKSPNFMELLTTVPGIDMISKGNGVA
                     KPVIRGLSMNDVLVLNNGVRFENYQYSNHHPLGIDEFGIETVEVIKGPASLLFGSDAI
                     GGVLNFIKERPATVGKLEGDYNLQLFSNSLGMVNNLGFKVSDNQFHGGIRVGQKTHSD
                     YLQGGGEFVPNSRFYEHSVKLNGGFNGKMLSTNIYYDYNMQKLGLVEEEAINLIASRG
                     RKPSVYYQQFGTHLVSQQSKIFLGKTKVDFNTSLQSTELAHLGNPNEYELQMRLTTLI
                     YEAKVLFPSNPGNDYILGAQGVNQWNININNRETILLPNAFSSGYSIFGLAQRSFNRF
                     RFQAGLRFDAKQIKSNEVGIPSEPEYRASVDRKYNNITGSVGATYRLNEYFLIRANGA
                     AAYRNPNLAELTSNGPHEGRYEVGNISLKPEKSVEYDVSAHYHNDNFLADVAGFVNSI
                     GNYIYIAPTGALNGGLPEYRYSQDDAYLYGGELGLHFHPKSVQWLHFKTNYSLVIGKK
                     RNGEYLPFIPANKLTFETGFMGKKFALGSKPYLLIAINYAFAQNNIDPGETVTPDYIL
                     VNLSMGSSFRLGNQPLEVNLTVQNLLDKKYVDHLSTLKEVDLYNPGRNISLSLRIPFR
                     ASIR"
     gene            complement(5449..5772)
                     /locus_tag="PLW22_04160"
     CDS             complement(5449..5772)
                     /locus_tag="PLW22_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73932.1"
                     /translation="MKKGKTKHSLILLTLFLLPIVSKIGDSLFHHHNHKNEHHNSSIN
                     ITHYHDKCLVFQYELIVFDPKVVLPKVEIYLNIISEISDLITLNVGIDSFELIKIRAP
                     PFNIF"
     gene            complement(5824..6939)
                     /locus_tag="PLW22_04165"
     CDS             complement(5824..6939)
                     /locus_tag="PLW22_04165"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU73933.1"
                     /translation="MMKINILLIITFGVLLMSCSGKNNKSDENQPTDSLVVISVEQFK
                     AMNMAIGNPTMVKFASEIAVQGVIEPSPNAKAYITSPIGGVIKSVKVVPSSVVKKGQA
                     LIAIDGPEILNTQTLFVETYNQHRIAKANFERISQLSKEGIASQKELLQAESEYRILD
                     AKLHSHRILLSKLGLNPDEIIKGEFSNEAYLVSPIEGTVARVETSIGKPVTTYEPLAE
                     VINTQSLLLKFFIFLNQVNSIKPGQKVEISLPGGGKVYGSVISVGLDANTENKAVECF
                     ASIILPANLVLISGTRVSAKVFTNFVEGWALPRTALHEIETGHMVYTLNGSDSLSYFF
                     KKVPIQVGLIQDTIVQVFDSTLSNVLVKGGYELTVEE"
     gene            complement(6980..10126)
                     /locus_tag="PLW22_04170"
     CDS             complement(6980..10126)
                     /locus_tag="PLW22_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOU73934.1"
                     /translation="MIERIIRFSINSKLIIILLTLTIALFGVYALTQIPVGAVPDITN
                     NQVQVITTSRNLSTQDVEQFITYPVELEMSNLPGVKEIRSISKFGLSVVTIVFEENLG
                     TYLPRQLIAEKLKAAAANIPEGFGEPDMGPISTGLGEIYQYVLDVKPGYETRYTTTDL
                     RTIQDWFVKRQLSGIPGVVEVNTWGGYLKQYEVAVDPQKLAQYGVSIIDVLNALEQNN
                     GVAGGAYIERDNQSFFVRGEGLLKSLADIEQIPISTINGISVRIADVAKVQEGHANRF
                     GAITANGQGEKVMGQIMMLKNANSNKVIEAVKQRVAEVQKHLPEGVYINPIVERSELI
                     SKTTNTILENLIFGCLIVFLVVLLILGNLRAALVISSLIPLALLFTISAMYIFGIDAN
                     LMSLGALDFGIIIDGAVIIVEFIVMKLTLRSNEITGIENTERKKIFDTISFQGASKMM
                     NSAIFGQIIILIVFIPILSLAGVEGKMFRPMALSFSFALLGAMFFGFTWLPVAASLFL
                     RPQKSNWTVTRWVMWLLHSSYKPVIEWAYNNKRWVLGLAFAMLLFSGWVFTRLGAEFV
                     PTLDEGDFVIQPVLKTGTSLTRTTELTTMMEKILIEKFPEVDKIVSRIGAAEVPTDPM
                     SMEEIDMIIKLKPKKYWVSAKSKEELANRFKEALSVIPNVEYEFTQPIEMRFNELITG
                     VRADLAIKIFGEDLGVLNAKAIEVTKLIDGVPGAADIIMDKTAGLPQISIRYNRDKLS
                     VYGISVTELNRVVSAAFAGATAGNIFEGEKRFDLVVRLSDSYRHDIDNVKNLLVSVPG
                     GGLVPLGELAQVEYSKGPAKISRDNTHRRVVVSVNVRNRDLESVVKDIQKILDSKLRL
                     PAGYYVEYGGQFENLRNARKRLLVAVPVALALIFIFLHFAFHSLKDAILVYTAVPLSV
                     IGGILMLWFRGMPFSISAAVGFIALFGVAVLNGIVLIEHLKELKDEGVENLRERIMRA
                     TRDRLRPVLLTASAAAMGFLPMAISTSAGAEVQRPLASVVIGGLITSTLLTMIALPLL
                     YAVFERFNGLKFWNLFRK"
     gene            complement(10194..13367)
                     /locus_tag="PLW22_04175"
     CDS             complement(10194..13367)
                     /locus_tag="PLW22_04175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOU73935.1"
                     /translation="MRFTIVLSLLSLSVVSFAQRYNIEIIGLKNGLLHSLVTGIDQDT
                     QGKVWLSTGGGLCNYNGFEFKYLTTRDGLNYTRLTDVAVDAEGNIWASSSLGLNMVRG
                     NRILTIPKDTIGEVASLGKSSNGVWVLSNRGVFRVAFSKEKFDIIQYSLPVNLNVALS
                     PIFQDRPLTDFVFQSTLNKVYVGHKGTLYKLSNESFSPLTFEPSVYVNACSENANGDI
                     FVATSNGLYRLVDDKPVKVNHPYASRIDILKFVCIGSKIWCNGKLKGSDEVSIHAFDL
                     EDESFNRTLGVKNGLPDIPSKLYVDHEHNIWILTNNGIAILKGEAFTCLTTNDGLIGN
                     KIWGICRVSDGTLWAGTIGEGLSVISPTRIYQYSTKNGLPDNYVGKIFESSSRKIYVG
                     TSNAGLNLATYINSTLGYSFNRLPLLPNDRVRIDDIAEDRQKRIWVATSKGLFYATDG
                     IHFNHFPLFDSDTGGVFVQKLQIDTLRKRMWVGTRFNGIFFIENDKPFKFTGIDKNAE
                     ISTIVQDFKGDIWFGTRNSGVFHYDGSNIKQIKETDGVSSNLIYVLYADSDYLWIGTN
                     LGLDRLSLAEYSKGKIDLRHYGSSEGLPDLEINLNGVLADGYDGFWIATNGGLAHYQK
                     SLDKINNIPPKVSITNLLLRSQPTPWADYASDIDELTGLPSQLKLSYWQNHLTFEFAG
                     VSFKNPQGVKYAWFLEGLDNSWVESKNRQAVYSNLQPGKTYRLHLKAANSDGIWSGEV
                     ISMPIYIAPPFWATWWFRLLSIIAIGLLIYWYVNRRIQYLKERQEELEAMVEQRTVEL
                     REQLDIVDDKNRQIMDSIMYAKFLQTSMLPSVDDFKKYFNDAFIFYRPKDFVSGDFYW
                     FCHHKNVSVFAVADCTGHGVPGAIVSVICENALRNAVKECDYQNPAQILTITNAHVVE
                     FFTQSQKSIHDGMEIALAIFNHHTLELDFSGAKLPLYYISGGEVVKLKPDIYRIGWDK
                     EKVLYNNQRVKLSKNDLIFAFTDGFCDQFGAETGQKFSSARLKSLLVENNQQPHTYIE
                     QLLSITFDQWRGFSEQTDDVLVMGIKV"
     gene            13491..>14303
                     /gene="mnmA"
                     /locus_tag="PLW22_04180"
     CDS             13491..>14303
                     /gene="mnmA"
                     /locus_tag="PLW22_04180"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOU73936.1"
                     /translation="MERVVVGLSGGVDSSVAAYLLKQQGYEVIGMFMKNWHDTTGVLK
                     GDCPFDDDKQFAEMVARKLNIPFKFVDLSDQYRQRVVDYMFSEYSKGRTPNPDVLCNR
                     EIKFDAFLEAAIKLGAKYVATGHYCRKETITKDNTTLYRLLAGVDPNKDQSYFLCQLN
                     QQQLSKAIFPIGHLHKPEVRKIAAELGLATAERKDSQGICFVGKVDLPVFLQQKLKAK
                     KGKIVEIPADFYTQLSSINSNDLASLSKPYTYKLSNGKVVGEHQGAHFFTIGQ"
     assembly_gap    14304..14403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14413..14508
                     /locus_tag="PLW22_04185"
     CDS             14413..14508
                     /locus_tag="PLW22_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73937.1"
                     /translation="MFDTPQRGVTAGQFAAWYIDDELIGSGVIAK"
     gene            14719..17409
                     /locus_tag="PLW22_04190"
     CDS             14719..17409
                     /locus_tag="PLW22_04190"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOU73938.1"
                     /translation="MQNHILFGNNNGLLCFDGASWKIKPHPNNTTIRSVLTAKNRIYA
                     GSFEDFGYYEILNNSVGNYFSLAKPFRSSIHNEEFWRIVSIKDIIFFQSFGTIFYLSK
                     DRIGKIPLDFSVLLLMQAGENIFTQAINGPIYRVDTLGLKQIKGSEIFKNTEVKSVVV
                     LNSDTLFLTSTMGIFRFDGNAFTPWKTSIDIQLIKSNPNIAQSQGSKLAIGTILEGVF
                     IFDNKGKLLHHIDSKLIHNNTILSMQFDSIGNLWLGKDKGISYVAFNSPISTYVNEKE
                     SFACYSGALSQNKLFLATNQGVYTYSLNPRGEFENQGVIDGTQGQCWFTKQIGNKLYF
                     GLNNGTYSYSNGNLNKVCYISGGYNLERFGIEPSLFIQSTYRNLVTYSYENGSLRVNK
                     IIPTYSSPTRFLQIDFQNYLWLGHTVTGIYRAQISNDLSEIVKIDTIGIKNNLNINTN
                     RIYKIENRIVVPTQKNILRWDDISEQFIPFNEINYQLQGFEKCKAIISLPANHYWFIK
                     DDEWGLFEIRYGEAKLKYRILPQAYNIEMVDGYENIVSLNDSLQIICLDNGFSILNLK
                     MFDRIPIDIHPPIITEVVIWNGEQNVKSHPRLNRNNELTIPWRKNNVSFSFTSKDIIG
                     VKHFYQYMLDGVDREWSEWTPNTSIDYKRLSPGTYRFKVRTLDSKGHITPNATVTIKV
                     LPPLYASWKAIGFYILLGFAITAYTRARYKKKVKKHYEEKRKLELEKINKEKEEKEKI
                     IIKLQNEKLQADIENKNSQLALSTMAIVRKNQLLQDIAQELEGLKNELGYRIPNKYYS
                     KISRLITQDIESDHDWEIFEKLFDQAHQNFFRRLKENYPSLTPSDLRLCAYLRMNLSS
                     KEIAPLLNISIRGVEERRYRLRKRLGLDTNDNLNDFILKF"
     gene            17842..20868
                     /locus_tag="PLW22_04195"
     CDS             17842..20868
                     /locus_tag="PLW22_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU73939.1"
                     /translation="MHSLTQTKPKTLIRIAGTLLLVLLFSSSFAQQIIKGTVKAGDTG
                     EKLPGANVTVKGTTRGTITDLEGNYQIEVKPSDKILVFSFVGFEDTEVEIANQKVIDV
                     TLKLKSTQLQEVVVVGYGTQKAKDLTAPIVTVKGNELSKQVASNPMNALQGKVSGVQI
                     INSGEPGSGAAVKIRGVGSIGDYAKPLYVVDGVFVDNIDFLSSSDVEDLTVLKDASAA
                     AIYGVRAANGVIIVTTRKGKTGIPTVSYDSYVGFQVPVNILKMASKDQYITLLNEANE
                     NVPGYVPKNPADYPTSTDWYQELVRPAGMNNHNLDISGSTDKTSYSIGGNYIYQNGIM
                     DAKNNYQRYNIRGRLDQQVNKNLKIGINTIISNYSRFNPNTYAFFGAYVNPPVYSVYD
                     PTNSDAYPVKFGAPQKYGFGNEYGNPVASAYYTDNFEKGHKEVFSIYAELDIIKDKLT
                     YRAAYNQDQDNWNQRSYTPQFYVGGSQGVTKSSLTKTFGGASKQIIDNLLTYKNSAGK
                     TSYSILLGHSIRMEKWETLSGFANSVPGYDEQSKYLINGSFRDRNAYDGAARYNGLSF
                     FTRGTLNHNDKYLATLTFRADASSKYQQKWGYFPSLGIAWNITQENFSRINEKFANLK
                     LRLSWGMLGNDNVPANSSMILGQTGVGSSGVFGDNLVDGMGAQTVLQNYLKWETVTEF
                     DMGFDFTLKNQKVSGELDYYRRVTNNVVFYAPIATGGGIAELLGNNGKVLNTGVEFSL
                     NYKNEISKDLNYNISFNATFNHNEVLELNGREYIPSGYVRGNYTTRTAVGHPIGSFYG
                     YEIDGVYKSELEALRDPVSQAIKDKGYFKYKDQNGDKVVDANDKVYLGSAIPWLITGF
                     DFGLNYKKFDVSLSITGQYGNKILNAKRMNRDVFSDGNYDEDFYNNRWTPSNKSTDYP
                     SAEAYNSSFIQQANDFFVENGFYIRIQNVQIGYTTDKIPYVPKFRVFFTAQRPYTFFT
                     YKGFTPEVGGSPISSGIDFNVYPMQAIYTLGIKLIF"
     gene            20886..22373
                     /locus_tag="PLW22_04200"
     CDS             20886..22373
                     /locus_tag="PLW22_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOU73940.1"
                     /translation="MKTLKYIIITLASVLLLWNCEDFLDFRQEGTLPTTGIDYTKSEN
                     IFLSVSAAYAKLRNWGAHVFPYVGAFEIASDNADKGSTPEDNPEMRDIDNLNFQPNNG
                     LINALWTGYYDIVSGANYAIHQMPLFVQNLQNKSDRDYAIQCQGEAKAIRAYAYFNLT
                     RLFGRVPIVDTVLSSEQLASLSQASTSELYAFIEKDLEEAIAVLPDGYTKEWGGRISK
                     YTAMAIKAKVHLYQSEWDSVASLTDKIIASGRFRLLDNFREVFSMDGENSKESLFEIQ
                     SSTLGKSSGDAAYLEYGYVQGPRGNSPANMQGWGFCTPSQNLIDFYTSRGEIIRPATT
                     LLYRGTKTPEGDSIKMSCANPVYNGKVYTPSSQNRWVYNGYGFDHNIRVIRYSDILLM
                     YAEALVQGATVPLTCGLSADNAINMVRNRAGLPSISGATLQDIWDERRAELAMEEDRY
                     FDLVRTNQAATALASKGFVVGKHEHYPIPAAQMQLNPNLTQNNGY"
     gene            22420..23295
                     /locus_tag="PLW22_04205"
     CDS             22420..23295
                     /locus_tag="PLW22_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73941.1"
                     /translation="MKNKFRIFGALLFGAVVMGGLLFTACSKDDDDNDNGKVDPSTIA
                     TANLVAYMPFDDNALETIGNITPSQQPNVTYVVGRRGKAYQGASGAYLRYNLPDNSKL
                     KTLKSYTVAMWIKSPKVDGTPVPKVIEIGKSDDLFWGNLTIMIERLTDPGADSLFFKL
                     HFKKVGVPWEGQWIEAKKSIFQVNKWMHLVFQYDATTSKFMIYKDGAKVEFPEAVENR
                     YANDQQEPLGDLNFVNADVLNIGAWRPKTDSQATDEWMGWFMGNLDELRVYDKALSAT
                     EVKALYDAEVSQITE"
     gene            23347..24996
                     /locus_tag="PLW22_04210"
     CDS             23347..24996
                     /locus_tag="PLW22_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOU73942.1"
                     /translation="MKYVFSIIALTGILITVTFCKEEETVKPIGNIMVDSITIDGIKV
                     YDNGQINHIGFKPNIKVYFDKPVDTTKIDTYNIYFSGDTYNNYTHQFNTDSKVLSIIP
                     NENLKPLTLYRFTVSQGKNLGGNIINGYTAKFFTKLDSTDKFQQISDDSLLTLIQHRT
                     FKYFWDFAHPTSGLARERNTSGDIVTIGGSGFGIMAIIVGIERGFITRAEGISRFSTI
                     VNFLKDADRFHGAWGHWYNGVTGKVYPFSTNDNGGDLVETAFLVQGLITLRQYLNPNE
                     SSENNLINQINELINGVEWTWYTKDGEDVLYWHWSPTVGWAINMQIRGYNEALIVYVL
                     AASSTTYPINPSVYHSGWARNGAITNGKTFYGISLPVGFDYGGPLFFAHYSFMGLDPR
                     NLSDNYANYWQQNVNHTLINRQHCIVNPGRYPLYSADCWGLTASDDNTGYGVHEPTRD
                     IGVISPTAALSSMPYTPEESKKVARFLYYKLGNKVWGEYGFYDAFNVTANWWGTSYLA
                     IDQGPIVVMIENYRSGLCWNLFMSAPEVKQGLSRLGFNTSR"
     gene            25015..26418
                     /locus_tag="PLW22_04215"
     CDS             25015..26418
                     /locus_tag="PLW22_04215"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOU73943.1"
                     /translation="MAINGKIGMFWKVSMVLTFLMLSCSKTNNISFPSKGKINYSLTA
                     EEEKMLDSIQYKTFQYFMNEHHPHWGAVKDRAAKWSPISIAATGFAIPCFAIGAERKW
                     ISRDEAAQITLNILKFFKNSEQSPDPLSTGYKGFYYHFLNMETGKREWKCELSSVDTG
                     LLLMGIIFARNYYNANNSVERQIRQIADFLIKRVDWDFMQMPPQGQYAYTISMGWYPE
                     SSIHNMGWKGYNEALFLYILAAGSGMKNIEKAYNSWLESYNWYTPYKDFSHVAFPPLF
                     GHQFSHAFIDFRGIQDSYMSKKGIDYFINSRIATYAQREYCIDNPHGWVGYDSLCWGI
                     TACDGPTDKYNFDNKVFLGYAGRGASGRELNYFDDGTIAPYGHLSSLPFAPEIVLPTT
                     KAMLKRYGDKIWGKYGFYDSFNPTAKWVDNDFIGIDQGPMLIMIENFRTGMVWNYIMP
                     DPIIQSGLKKLGFQNIN"
     gene            26473..>28067
                     /locus_tag="PLW22_04220"
     CDS             26473..>28067
                     /locus_tag="PLW22_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOU73944.1"
                     /translation="MNHHLKATITAALLSVLTFHIGVKAQTVTLDQFESTEGWNYIKS
                     DGVNITAQTDNGLSGKSICITYNFVKGTGYCGIQKLFPINLPENYEITFYIKAESPSN
                     NFEIKFIDSTGNNVWWVNNRNYNFPTSWQKVRVKPRHISFAWGPTTDTRFRRIDRIEF
                     TVASFVGGSGKIWIDSLTFKALPPISNEYPAPVAYEISGTQSKPIAEILDQQPHSVWI
                     AKNKSIQEILIDFKALREIGGLKIDWMQGKQAQKFDVLLSYDSINWEKTFTVNLNINS
                     TSYIRLPEFETRFLKLKLSQSNNSKGLGIKDIEIIKVEESNTLNNFFRYIAKNSPKGY
                     YPRYFLDQASYWTISGVSGDAKEALINEDGMVEVEKASFSIEPVVKVGNKIYDWSNVK
                     TSQSLTYFIDGKTYDYTPTVNWHTDDIDLQVGITSYGNANNSSKLLIGYTLKNNTNKN
                     QTIDFYLLIRPFQVNPYYQTLNLAGGVGTIKKISLDASKDRVEVDNTSLYFTQKFESF
                     FAGSFYSANPTELIRSNHKEVVMA"
BASE COUNT         8829 a         5860 c         5374 g         7904 t
ORIGIN      
        1 acgatggata tcatcctaag gatgatacct cgtgtgtaac aatatacttc cgtaacccca
       61 gaaaactcct tatttgcact ggccctccct acgaaaagga gaacgacacg aagcttgcca
      121 agatatttga cacctttgag gggaaaaagg caatttgcgg agctactaca gttgatatca
      181 ttgcccggga gctaaaacga agtgttgagg atagcttaga atttaccgat cctgatttgc
      241 ctccaattag ccaaatggag ggagccgatc ttgttaccga aggtatttta acccttagca
      301 aggttaacac cattcttgaa tcgtataacg agaacacaaa ccttggtaaa ggccctgccg
      361 atgaacttgt taaacttatt ttggaaagcg atagcattga atttgtgatt ggaacctgta
      421 taaacgttgc acaccaggac ccaaacttac ctgttgaact ggaaatacga aggactgtgg
      481 taaagcgaat tgccaatata cttcaggata agttcttaaa ggaggttaaa ctccagttcc
      541 tatagaaggc ttaggctgct tttaccattt ttggaatatc aatattttaa cctaacaagt
      601 aaataattag ttcaactgtt gacttttacc aacattttta caatatttac gttaaaatca
      661 ttgttaatcc ttaaaactat gcgcagcttt aggtttttcc ttttgaacat gcttattgct
      721 acttactttt cagctgtaag ttacggccag gattggaatt caattttcac tctggaaacc
      781 gacgctgaat actacatggc tgagggaaac ttttctaagg cagccgacac ctatgtaaaa
      841 gccctaaaaa aatttcctga aagtgccaac cttaaattta aaattggata ctgcttgctc
      901 aaaacccccg acagaaaatc ggaagccatt agctaccttg aagaggctgt aatgaaaatt
      961 tcggataaat atgatgcaaa atcattaaag gagcccaacg ccccccccga agccctttat
     1021 cagcttggta tgtcatacat gtatcaaaat gattttgaca atgcgctaaa atcgttcaga
     1081 gagtatcagg gttacatcaa acctaaagat gaacatgcta aactcatggc cgagcatgca
     1141 atagcgagtt gtgaaaatgc aaagaaattt caaaaaagcc caataggcat taaatacaac
     1201 cggttaggtc ctgattttaa taatgaaaac gacaacttta atgctgtggt atcgggcgat
     1261 ggaagtacgc ttgcttacac tacccgcacc tcctcaggca ataaaatatt tgttgccaaa
     1321 aaaggggagg atggcttgtg ggctaaacca gttgatataa cccgcaacct gggtagtaaa
     1381 aacctgacca catcatacct atcgtatgag ggaactgaac tttacctcat agagaatgac
     1441 cctagaaatt cggaaattgt agtaagtttt ttacagaaac gaaaatggtc atcgcccgta
     1501 aaggcagcca aacccattaa ctcaaaattc aacgaaaccc atgtatgcgt tacccgcgat
     1561 gggaataccg tttacttcac tagcgatcgc aagggcggtc agggcggata cgacatatgg
     1621 gcttctacaa tcaataacgg tagatggagc gatcccataa accttggccc caacgttaac
     1681 acccctttcg atgaggcaac cccattcctt tcacccgatg aaaagtatct ttttttcagc
     1741 tcacaggggc acaataccat gggcggattc gatgtgtttt ttaccaacct acaaggaagt
     1801 cctaatgtta agaatgcagg gtatccttta aatactaccg acgacgaaat cttttacttt
     1861 ccaaccggct tatcatcggg ttatattgca aaattcagtc ccgatggttt tgggaaactt
     1921 gacattgccg aggttgaaat aatccccatg gttgatgtta agcttaacct catgcttgcc
     1981 gacaatgctc ctctggataa atactacaac gttaacgtta caaataattc tacaggtgag
     2041 atagtagaaa aacagtcagt aaaaggtaaa acacagctta gcctaaaggt aaaacccggc
     2101 gattatactg taactgctca gggcgaaggc tttgaggaaa ctaaaacccc acttagcatt
     2161 ccagccaagc ccgataaatc ggaataccag gcaagcctaa tgttaaaccc gagagtagtt
     2221 cagcagcccg ttgcccaact tgaacccccc aaggaagaac ctaagcccga agttaaacct
     2281 gaggtaaaac ctgaacccaa gcctgaagta aaacctgagg ctagagctac cgataaacct
     2341 aaggctgttg aacccgaacc caaaaaagaa ccggttgtaa aacaagcccc aaaaaaggaa
     2401 ccaaaacctg aagttgaaaa gccaaagcct gaaccaaagc ctgctaaggc attgccccca
     2461 acccctcccc caacagcagc accaacaccc agaatggact tcaataaaac ctcatcaaca
     2521 agaactcttg ctaaaccaac atactctgtg cagctaatgg cctcactata tcctgttgac
     2581 tataattact ttaagctcga cagcattagc gtaaccattt cgcccgatgg ttactatagg
     2641 tatagtgtag gaaatactga taatatcaaa gagattgaag agctacagca gaaagttcgt
     2701 ggtcacgggt atagcaacgt atgggtaaga ataaatcggg aacacccggg atataccatt
     2761 caattcctag cattacagaa gcctaaaagc ataaagcagt ttactgaact ttccgatgta
     2821 atggtttacc gtggaaacga tggagtgtat cgttactgtg ttggaaagta ttccacaccc
     2881 gaagaagcct cttcggacct agccaagatt actggattag gctattcggg ggcatttgtc
     2941 cgtgaactag ggaaataaag tttagaaaga aagtataaag gctgcctaaa tattaggcag
     3001 ccttaaatat ttaacgaatt gaggcccgaa atggaattct caatgataat gaaatatttc
     3061 gaccaggatt ataaagatca acctccttta atgttgacaa gtgatcaaca tactttttat
     3121 cgagtaaatt ttgaactgtt aaatttactt caagaggttg attgcccaac ctaaaactac
     3181 tccccattga taagttaact aatatataat ctggcgttac tgtttcacct ggatctatat
     3241 tattttgggc aaacgcatag ttaatcgcta tcagcaagta tggtttactt cctaaagcaa
     3301 acttctttcc cataaaaccg gtctcaaaag ttagcttatt agctggaata aaagggaggt
     3361 attctccatt acgtttcttg ccaatcacca aagaataatt agttttaaaa tggagccact
     3421 gaaccgattt ggggtgaaaa tgaagaccta attcacctcc atacaagtaa gcgtcatcct
     3481 gcgaataccg atactctggt aagcctccgt tcaatgcacc agtgggagca atgtaaatgt
     3541 aatttcctat tgaattaaca aaaccggcaa catcggctaa aaagttatca ttgtgataat
     3601 gagcactgac atcgtactca acagatttct caggtttaag ggaaatgttg ccaacttcgt
     3661 agcgaccctc atgggggcca tttgatgtaa gttctgctag gtttggattt cggtaagcag
     3721 cagcgccatt tgctcgtatt aaaaagtatt catttaatct ataggttgcg ccaacagaac
     3781 cagtaatatt attatacttc ctgtctacac ttgctctata ctcaggttca cttggaatac
     3841 caacctcatt ggatttaatt tgctttgcat caaacctcaa tccagcttga aatcgaaacc
     3901 tattaaagga acgttgtgct aatccaaaga ttgagtatcc tgaactaaat gcattgggaa
     3961 gaagtatggt ttcgcggtta ttgatgttta tattccattg gttcacaccc tgtgcaccta
     4021 aaatgtaatc gttgcctgga tttgaaggga acaatacctt agcctcgtaa attaaagttg
     4081 ttagacgcat ttgtaattcg tactcatttg ggttacccaa atgggcaagt tcagttgatt
     4141 gcaacgaagt attaaaatcg acctttgttt tacccaaaaa gattttggat tgctgggaaa
     4201 ccagatgggt gccaaactgc tggtaataaa ctgagggttt tcgtccccga ctagcaatta
     4261 aattaatggc ttcctcttcc accagtccta gtttttgcat gttgtagtca taataaatgt
     4321 ttgttgacaa catttttcca ttaaaacccc cattgagttt aaccgaatgc tcataaaatc
     4381 tggagtttgg aacaaactct cccccaccct gcaggtaatc ggaatgagtt ttttgaccta
     4441 cccgtattcc tccatgaaat tgattatctg aaactttgaa tcctaaatta ttgaccatac
     4501 ccaaagaatt tgagaatagt tgcaagttgt agtccccctc cagcttacca acagtagccg
     4561 gtctctcttt aataaagtta agtaccccac ctatggcatc ggaaccaaaa agtagcgagg
     4621 caggtccttt tatgacctca accgtctcaa taccaaactc atcgattcct aatgggtgat
     4681 ggtttgaata ctgataattt tcgaatcgaa cgccattgtt gagcaccaac acatcgttca
     4741 tggatagacc cctgataacg ggtttagcaa caccattccc tttacttatc atgtcgattc
     4801 caggcaccgt agtaagcagc tccataaaat tcggcgattt tatcaagtga tgctcattta
     4861 acctcagaac ttcaatcttt actgcattct ggtgctgtgt tgtactatat ccagtggata
     4921 caaccaccct ttcggcctca acacttgcag gaataagaac aacatctaat tcgaattgcg
     4981 tcttccctaa agtaaccggt acaatttgac tagcgtatcc aatatgcgaa atctgaatat
     5041 caaacttacc gtttggtaaa cccgtaagtt caaattctcc gcttgaattt gttgaggtac
     5101 cttttaccaa cattggaata aaaacattag ctccctcaag gggaatcttg ttctcattgt
     5161 atactattcc tcttataata ttttgcgcca aaacattatt tccaaggaca aatacagtta
     5221 ttatgaataa ctttattaat ctcatgtcaa aatattttta ctagtttatt tactttttaa
     5281 ctaaacaact tttaaactgg catgatacca gcatatcaat tagattaaaa aaaaacttca
     5341 tatgcgatta caacataaca atcaatgtta tatcataata catccaatac agttaatcat
     5401 tgattacttg agtaaattat aaccccaata tgttaatatg aaagattact agaatatatt
     5461 aaatggtggc gcacgtattt ttattaattc aaaactatca ataccaacat tgagagttat
     5521 taaatccgat atttctgaaa taatatttaa ataaatctca accttaggta aaacaacttt
     5581 agggtcaaaa actattaact catactgaaa aactaaacat ttatcgtggt aatgagtaat
     5641 atttattgat gaattatggt gctcattttt atggttatga tgatgaaata gtgaatcgcc
     5701 tatctttgaa acaataggta gcagaaaaag tgtcagaaga ataagggaat gttttgtttt
     5761 gccctttttc attaaataga tatatttatc ttaaaaccta ccaaaaatcg atttgttcaa
     5821 ttgctattct tcaacagtaa gctcataacc cccttttaca agcacatttg aaagggttga
     5881 atcaaatact tgaacaatgg tgtcctgaat taacccaacc tgtataggaa cttttttaaa
     5941 aaagtagctc agtgaatcgc ttccattcag ggtgtaaacc atatgcccag tctcaatttc
     6001 atgcaatgct gttcttggca gtgcccaacc ttcaacaaaa ttggtgaaaa ccttagcaga
     6061 aacccttgtg ccacttatca aaactaaatt tgcgggtaaa attatagatg caaaacattc
     6121 aacagcctta ttttcagtgt tggcatcgag cccaacagaa ataacgcttc cataaacttt
     6181 accacctcca ggcaaactaa tttcaacttt ttgtccgggt tttattgagt tcacttggtt
     6241 cagaaaaata aaaaacttta atagtaggct ttgggtatta attacttcgg ccaagggctc
     6301 ataggttgtt acgggcttac caattgatgt ttctacccta gcaactgtgc cctcaatagg
     6361 ggaaaccagg tatgcctcgt ttgaaaattc tcctttaatt atttcatcgg gatttagccc
     6421 taacttacta agcaaaattc ggtgggagtg tagcttggca tcaagaattc ggtattcgct
     6481 ttcggcttgt aaaagttctt tttgcgaggc tattccctcc tttgatagtt gtgatatcct
     6541 ttcaaagtta gcctttgcta ttctatgctg attgtaggtt tcaacaaaca aggtttgagt
     6601 atttaaaatc tcgggtccat ctattgcaat aagtgcttgc cccttcttta ctactgacga
     6661 tgggactact ttcacagact taataactcc accaatgggt gaggtaatgt aagctttggc
     6721 attggggctt ggttcaataa cgccttgtac ggcaatttcg ctggcaaatt ttaccattgt
     6781 aggattgcca attgccatat tcattgcttt aaactgctca acagaaatta caaccaagct
     6841 atcggttggc tgattttcat cgcttttatt attttttcca gagcaagaca ttagcaatac
     6901 tccaaaagtt ataattaata aaatattaat cttcatcata cctcaactta aaaatattta
     6961 acttttaaac cttacatagt tacttgcgaa aaagattcca aaactttaac ccattaaaac
     7021 gttcaaatac tgcataaagc aacggaagcg ctatcatggt aagtaaagta gaagtaataa
     7081 gcccacctat tacaaccgat gcaagcgggc gttgcacctc ggcgccagcc gatgtagata
     7141 tagccatggg taaaaagccc atggcagcag ccgaagcggt aagtaaaacg ggtcttaacc
     7201 tatcgcgggt tgctctcatt atgcgttccc tcaaattttc tactccctca tcctttagct
     7261 ccttaagatg ttcaataagc actattccgt taagaactgc aaccccaaag agagcaataa
     7321 aaccaaccgc cgccgaaatg ctaaagggca ttcccctaaa ccatagcatc aatattccgc
     7381 ctattacaga aaggggcacc gcggtatata ccaggatagc atccttaagc gaatgaaaag
     7441 caaagtgaag gaaaataaaa attaaagcca aggctaccgg aacagccact aacaatcgct
     7501 tgcgagcatt tcgaagattc tcaaactgac cgccatactc aacataataa ccagcaggca
     7561 acctaagctt actgtcaaga attttctgta tgtccttaac aaccgattcc aggtcgcgat
     7621 tgcgaacatt aacacttact acaaccctgc gatgagtatt atcgcgggaa atttttgcgg
     7681 ggcctttgga atattcaact tgagccagtt cacccaaagg tactaatccc ccaccaggaa
     7741 ccgatactaa aagatttttt acattgtcaa tatcatgtct atagctatcg gataatcgga
     7801 ctaccaaatc aaaacgcttt tcgccctcaa aaatattacc agctgttgcc cctgcaaatg
     7861 ctgccgagac cactctgtta agctctgtaa ccgatatacc ataaaccgaa agtttatcgc
     7921 ggttatacct gatgctaatt tggggtaatc ctgccgtttt atccattatg atatcagcag
     7981 ccccgggtac accatcaata agtttagtaa cctcaattgc tttggcattg agcacaccca
     8041 aatcctcgcc aaaaattttt atagccaagt cagctctaac ccctgttatt aactcattaa
     8101 agcgcatctc aatgggttgg gtaaactcat actctacatt tgggattacc gagagtgcct
     8161 ccttaaaccg gttagcaagt tcctccttgc ttttagcgct aacccaatac tttttgggtt
     8221 taagtttgat tatcatatca atttcttcca tcgacatggg gtcggtgggc acctcggctg
     8281 caccaattct actcacaatc ttatcgacct caggaaattt ctcaatcaga atcttttcca
     8341 tcatggtggt tagctctgtg gttcgggtta atgaggttcc cgtttttagc acaggctgaa
     8401 taacaaaatc gccctcatca agtgttggaa caaactctgc acctaggcgg gtgaaaaccc
     8461 aaccggaaaa aagcaacatg gcaaaggcta aacccaacac ccatcgttta ttattatatg
     8521 cccattcaat tacaggttta taggaggaat gcagcaacca cataacccat cgggtaacag
     8581 tccaattact tttctgcgga cgcagaaaaa gcgatgctgc cacgggtaac caagtaaacc
     8641 caaaaaacat agctcctagc aacgcaaagc taaacgacaa ggccattggc ctgaacatct
     8701 ttccttcaac tcctgcaagc gaaaggattg ggataaatac tataagtatg atgatttgac
     8761 caaatattgc cgagttcatc atttttgatg cgccctggaa cgagatggta tcaaaaattt
     8821 tcttacgctc agtgttttca ataccagtaa tttcattgga cctcagcgtg agtttcatta
     8881 caatgaattc aacaataatt acagccccat ctataataat tccaaagtca agcgcaccaa
     8941 ggctcatcag gttggcatcg ataccaaaga tatacatagc tgaaatggtg aatagcaatg
     9001 ccagtggaat aagcgatgaa atcaccagtg cggcccttaa attcccgagt atcagtaaaa
     9061 ccaccaggaa aactatcaag cagccaaata tcaggttctc aagtatagtg ttagtagttt
     9121 ttgagattaa ctcgctgcgt tccacaatgg ggttaatata aaccccctcg ggtaaatgct
     9181 tttgtacctc ggcaacacgc tgcttaaccg cttcaatcac tttattagag ttagcgtttt
     9241 taagcatcat tatttgcccc ataacctttt cgccctgacc atttgcagta atggcaccaa
     9301 acctgttggc atgcccctcc tgcaccttag ccacatcggc aatacgaact gaaataccat
     9361 taattgtgga aataggtatt tgctcaatat cagcaagtga cttcaacaac ccctcgccgc
     9421 gaacaaagaa actttgatta tcgcgctcaa tgtaagcacc accggctacg ccgttgtttt
     9481 gttccaaagc gttaagaaca tcaatgatag acactccata ctgggctagt ttttgcggat
     9541 cgacagccac ctcatactgt ttgaggtatc caccccaggt attcacctca acaacaccag
     9601 gaatgccgga tagctgacgt tttacaaacc aatcctgaat ggttcttagg tcggtggtgg
     9661 tgtacctggt ctcatatcct ggctttacgt cgagcacata ctggtatatc tcccccaaac
     9721 cggttgagat aggccccatg tcaggttcac caaaaccttc cggaatattg gctgctgccg
     9781 ctttaagctt ttcagctatt agctgccggg gcaggtaagt tcccagattc tcctcgaaca
     9841 caatggttac aacagataag ccaaattttg aaatcgatcg tatctctttc actcccggaa
     9901 gatttgacat ctcgagctca accgggtagg tgatgaactg ctcaacgtcc tgggtcgata
     9961 ggttgcgact ggtggttata acctgaacct ggttattggt aatatctgga accgcaccaa
    10021 cagggatttg ggttaaagca taaacaccaa atagcgctat ggttagtgtt agtaagatta
    10081 taattagctt gctgttaata ctaaatctta taatcctttc aatcatctca atgcatattt
    10141 taacatatct atattaaaca atatatcttt ttgaatggtt caaaaaggca ttattaaacc
    10201 ttgattccca taactaaaac atcgtcagtt tgctcactaa aacctcgcca ttgatcaaac
    10261 gtaatggata gcaactgctc aatgtatgta tgcggttgct ggttgttctc aaccagtaaa
    10321 ctcttaagac gtgccgacga gaatttttgt cccgtttcag ccccaaactg atcgcaaaaa
    10381 ccatcggtga atgcaaaaat cagatcgttc tttgagagtt ttacccgttg attattgtaa
    10441 agtacctttt ccttatccca cccaatgcgg tagatatcag gttttaattt taccacctcc
    10501 ccaccgctga tgtagtaaag gggtagctta gctccgctaa aatctaactc caatgtatga
    10561 tggttgaaaa tagccaaggc tatctccatg ccatcgtgaa tgcttttttg ggattgggta
    10621 aagaactcaa caacgtgagc gttggtaatg gtaagaattt gtgcaggatt ttgataatca
    10681 cactccttta ctgcattcct aagagcattc tcgcaaataa cgctgacaat ggcaccggga
    10741 acaccatgcc cagtacaatc ggccacagca aaaaccgata cgtttttatg gtgacaaaac
    10801 cagtaaaaat cgccactaac aaaatccttg ggacggtaaa agataaaggc atcgttaaaa
    10861 tatttcttaa aatcatctac actaggcaac attgatgttt gcaaaaactt tgcatacata
    10921 atactatcca ttatctgcct gttcttatca tcaacaatat cgagttgttc cctgagctcc
    10981 acggtgcgct gttcaaccat agcttcaagt tcctcttgcc tttcctttaa atattgtatc
    11041 ctacgattca cataccaata aattaacaac cctatggcaa tgatggataa aagcctaaac
    11101 caccatgttg cccagaacgg gggagcaata tatattggca tcgatataac ctcgcccgac
    11161 caaatgccat cgctattggc tgctttcagg tgtaaacgat aagttttccc gggctgtagg
    11221 ttactataaa ccgcctgccg gttcttgctc tcaacccaac tgttatcaag tccttccaaa
    11281 aaccaggcat actttacacc ctgtgggttt ttaaaactaa cacccgcaaa ttcaaaggtt
    11341 aaatggtttt gccagtaact aagttttagc tgggaaggga gtccggtaag ctcatcaatg
    11401 tcagaggcat aatcggccca aggggtgggt tgcgagcgta ggagtagatt ggtaatactc
    11461 acctttgggg gaatgttatt aattttatcg aggctcttct ggtaatgggc aagtccgccg
    11521 tttgtggcta tccaaaagcc atcatatcca tcggctaaca cgccatttag gttgatttct
    11581 aaatcgggta aaccctcgct ggagccataa tgcctcaaat ctattttgcc cttgctatac
    11641 tcagcgaggc taagcctatc caatcccagg ttggttccaa tccaaaggta atcgctatcg
    11701 gcatagagta cataaatcag atttgatgaa acaccatcgg tctccttaat ctgctttatg
    11761 tttgaaccat catagtgaaa aaccccgcta ttccgtgttc caaaccatat gtcaccttta
    11821 aaatcttgta ctattgttga tatttcagcg tttttatcga taccagtaaa cttaaagggt
    11881 ttgtcgtttt caataaagaa aataccgttg aaccgggttc ctacccacat tcgctttctt
    11941 aaagtatcta tctgtaactt ttggacaaaa acgcctcctg tatcgctatc aaaaaggggg
    12001 aaatggttga aatgaattcc atcagtggca taaaacaagc ccttagatgt ggcaacccaa
    12061 atcctttttt gccggtcctc ggctatatca tcaattctaa ccctatcatt tggaagcaat
    12121 ggaagcctat taaaggaata gcctaaagtg gaatttatat aagttgcaag gtttaacccg
    12181 gcattgcttg taccaacata aattttcctg ctggacgatt cgaatatctt gcctacatag
    12241 ttatcaggaa gtccattttt agtggaatac tggtaaatcc tggtagggct aataactgat
    12301 aaaccctcac cgatggttcc tgcccataaa gtaccatcac ttaccctgca aatcccccat
    12361 attttattgc caattaaacc atcgttggta gtaaggcagg taaatgcttc tccttttagg
    12421 attgctatgc cattattagt taaaatccaa atgttatgtt catggtcaac atatagcttt
    12481 gaggggatat cgggcaagcc attcttaaca cctaacgttc ggttaaaact ttcatcttca
    12541 aggtcaaaag catggattga aacctcatca gaccctttaa gctttccgtt acaccaaatc
    12601 ttactgccaa tacaaacaaa ctttaatata tcgatcctgc tggcatacgg gtgatttact
    12661 tttacaggtt tatcatcaac tagcctatat agcccattac tggtagcaac aaaaatatcg
    12721 ccatttgcat tttcactaca tgcattaaca taaaccgagg gttcaaacgt taaagggctg
    12781 aaggattcgt tactcaactt gtaaagagtt cccttatgcc ctacatatac cttgtttaaa
    12841 gtggattgaa aaacaaaatc ggttaaaggc ctgtcctgaa agataggact taatgcaacg
    12901 tttaagttga ccggtagtga gtactgaata atatcaaatt tttccttgct aaaagcaacc
    12961 ctgaacacac ccctattgct taaaacccaa accccattgc ttgatttacc aagtgaggca
    13021 acttcgccaa ttgtatcttt gggaattgtt aaaattctat tacccctaac catgtttaat
    13081 cccaacgatg agcttgccca gatattgcct tcggcatcaa cagcaacatc ggttaagcgg
    13141 gtatagttga gcccgtccct tgtagtcagg tatttaaact caaatccatt gtagttacaa
    13201 agacctcctc cggttgacaa ccatactttc ccctgggtat cctgatcaat accagtaacc
    13261 aaggaatgca gcaagccatt ttttagacca atgatttcaa tattgtatcg ctgtgcaaac
    13321 gacacaaccg acaggctgag tagagataaa actattgtaa aacgcattaa atgataggct
    13381 taaaatttaa tccattaaat ttataaaatt gaagaattta ttcctattac ttacataaaa
    13441 atctgactaa aaaatagtaa ttgctaaatt tgcagcacta aactaaggaa atggaaagag
    13501 ttgtagtagg cctttcgggt ggtgtagatt caagcgttgc tgcttatcta ttaaagcagc
    13561 aaggctacga ggttattggg atgttcatga aaaactggca cgataccacg ggtgtactta
    13621 agggcgattg cccttttgac gatgataaac aatttgctga gatggttgcc cgtaaactta
    13681 atatcccttt taagtttgtt gacctcagcg accagtaccg ccagcgcgta gtagactaca
    13741 tgttcagcga gtattccaag ggtcgcacac caaaccccga tgtactttgc aacagggaaa
    13801 taaagtttga tgcttttttg gaagcagcca taaaacttgg tgcaaagtat gtggcaaccg
    13861 ggcattactg cagaaaagaa actatcacaa aagataacac tactctgtac cgcttacttg
    13921 ccggagttga tcccaataag gatcaaagct actttctgtg ccagctaaac cagcaacagc
    13981 tgagtaaagc gatctttccg attggtcatt tgcataagcc cgaggttagg aaaattgcag
    14041 ctgaacttgg cctagcaacg gcagagcgga aggattcgca gggaatatgc tttgtaggga
    14101 aggttgattt accagtgttc ctgcaacaaa agcttaaagc taaaaagggg aaaatagttg
    14161 aaatacctgc cgatttttac acccagttgt cgagtattaa tagtaacgat ttagcatcgc
    14221 tttcaaaacc atacacctat aagctttcca acggaaaggt agttggcgag catcagggcg
    14281 ctcacttttt tactattggt cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnntactacc tgatttttga tacccctcaa cgaggagtaa cagcagggca atttgcagca
    14461 tggtacattg acgatgagct gattggttct ggtgtaatag cgaaatagtg gttgaatatt
    14521 tgacgatatt tgatatattt gttcaaggat taccacaacc taatagtaaa agccttgaac
    14581 cgtcagttta gtttcctgat agtaaatatt ttgctgacat taagtagtta tccttactgt
    14641 caaagttcgc ttcttataaa taactttgag aaaaaggact atcgggcagg aaatcagaac
    14701 tggtctatct gccttggcat gcaaaatcat attctgtttg gtaacaataa tggactgctt
    14761 tgctttgatg gtgctagttg gaagattaaa cctcatccga acaatacaac aatacgctca
    14821 gttttgacag ctaaaaatag aatttacgcg ggaagctttg aggactttgg ctactatgaa
    14881 atattgaaca atagtgttgg caattacttt tcgcttgcta agccctttag atcgagtata
    14941 cacaatgagg agttctggcg tattgtatca atcaaggata ttattttttt tcaaagcttt
    15001 ggaacaattt tttacctgtc aaaggataga attggtaaaa tacccctgga tttctcagtt
    15061 cttctgctaa tgcaagccgg tgaaaatatt tttactcaag ccattaacgg accaatttac
    15121 cgtgttgata cactaggcct caaacagatt aaagggagtg aaattttcaa aaacaccgag
    15181 gttaaatcgg ttgtagtgct taatagcgat acgctttttc tgacttcaac aatgggaatt
    15241 tttaggtttg atggaaacgc tttcactcca tggaaaacct ccattgatat tcaacttata
    15301 aaatcaaatc caaacattgc ccaaagccaa ggaagtaaac ttgccatagg tacaatactt
    15361 gaaggtgttt ttatttttga taacaagggg aaactattac atcacatcga ctcgaagcta
    15421 attcataaca atacaatcct ctccatgcaa ttcgactcaa ttggaaatct ttggttagga
    15481 aaagacaagg gtataagcta cgtggccttt aactcaccaa ttagcacata tgttaatgaa
    15541 aaggaaagct ttgcgtgcta ctctggagcc ctttcacaga ataaactatt ccttgcaaca
    15601 aaccaaggag tatataccta ctctctcaac ccaagaggcg aatttgagaa ccagggtgtg
    15661 attgacggaa cgcaaggtca atgttggttt accaagcaaa ttggtaacaa gctttacttt
    15721 ggtttaaata atggtaccta ctcatattcc aatggcaatc tgaacaaagt atgctatatt
    15781 tcaggcggtt ataatcttga acgatttggc atagaaccta gtttattcat tcaaagcacc
    15841 tacagaaatt tagttactta ctcatacgag aatggttcgc tcagggtaaa taaaataata
    15901 cccacatact catcgcctac acgctttttg cagatcgatt ttcaaaacta cttgtggcta
    15961 ggacacacag ttacaggaat ttatcgtgct caaatcagta acgatctttc agaaattgtg
    16021 aaaattgata ctataggaat aaaaaacaat ctgaatatca acacaaacag aatatataaa
    16081 attgaaaaca gaattgttgt accaactcaa aaaaatatct taagatggga cgatatttcc
    16141 gagcagttca taccctttaa cgaaatcaat taccagctac agggttttga aaaatgtaaa
    16201 gcaataattt cgctccctgc aaaccattac tggtttataa aagatgatga atggggttta
    16261 tttgaaatac gctacggtga agctaaactt aagtatagaa tattgcccca agcctacaat
    16321 attgaaatgg ttgatggata cgaaaatatt gttagcctta atgatagcct tcaaattatt
    16381 tgcttagata atggtttctc cattcttaac ttaaaaatgt tcgatagaat tcctatcgac
    16441 attcaccctc caattattac agaggttgta atttggaacg gcgaacaaaa tgtaaaaagc
    16501 cacccccgct taaatagaaa taatgaactc acaatcccat ggcgaaaaaa taacgtttca
    16561 ttctcattta cttcaaagga tattattggt gttaaacact tctaccagta tatgcttgat
    16621 ggtgttgacc gtgagtggag cgagtggacc ccaaatacat caattgacta caaacgactt
    16681 tcgcccggta cgtacaggtt caaggttaga acgctagatt caaagggtca tattacccca
    16741 aacgccaccg ttacgataaa ggttttacct cccctttacg catcatggaa ggcaattggt
    16801 ttttacatac ttcttggttt tgcaattaca gcttacacaa gagcaaggta taaaaagaaa
    16861 gtgaaaaagc attacgaaga gaaacgtaaa cttgaacttg agaaaattaa caaggaaaag
    16921 gaagaaaaag aaaagattat cattaaactt cagaacgaaa agttgcaagc cgatattgaa
    16981 aataaaaact cgcaattagc gttgagcaca atggcaattg tccgcaaaaa ccaactctta
    17041 caggatattg cccaagaact tgaaggccta aagaacgaac tggggtaccg aatcccaaat
    17101 aagtactaca gcaaaataag caggttaatc acccaagaca ttgaaagcga tcacgactgg
    17161 gaaatctttg aaaaactatt cgatcaggcc catcaaaact tctttagacg attaaaagag
    17221 aattacccat cgcttacacc aagcgacttg cggctttgcg cataccttcg catgaatcta
    17281 tcgtcaaaag agattgcacc cctgcttaac attagtatcc gtggggtaga ggaacgaagg
    17341 taccgattaa gaaaacgatt aggactcgac actaacgata atcttaacga cttcatactt
    17401 aagttttagt agggccaaac tatatttcat ttcaatcttt caatagttct aacaaaatat
    17461 ttagggctaa ctattttctt tttctttaac ttctctaacc ctatcatatt ccatctattc
    17521 cgactcattt atttatgctt caggctttcc gttttattag tcctgtttaa tttgttaaat
    17581 catggcactt gggtgattat tagattaatt tcttcaaatc atgctttgta aaattttaca
    17641 aaacattctg ttgtatttat ttgtttacca attatttaac attcctttaa tttaaagtga
    17701 tgtagtattg agcaacggaa tccaggatag attttcgacc cccgtaatta atgatgatgt
    17761 taaatagtgg actttagcta acccaaacta taattttggt tgcgcaaacg ttttaaacta
    17821 attgttcaaa aaacaaaagc tatgcacagc ctaactcaaa ccaaaccaaa aactttaata
    17881 cgcatagcgg gaactttatt gcttgttcta cttttcagtt catcatttgc acagcaaata
    17941 attaaaggaa ctgtaaaagc gggcgataca ggcgaaaaac ttcccggtgc aaacgttact
    18001 gttaagggta caacccgagg tactattacc gatttggaag ggaactacca aattgaagtg
    18061 aaacccagcg ataagatctt agtgttctca tttgtaggat ttgaggatac agaggttgaa
    18121 attgcaaatc agaaggtaat agatgttaca ctaaaattga agtcaaccca acttcaggaa
    18181 gttgttgtgg ttggctatgg aacccagaaa gccaaggacc ttactgcccc aattgtgaca
    18241 gttaaaggta acgagctatc aaaacaggtt gcatcgaacc caatgaatgc cttgcaaggc
    18301 aaagtctccg gtgttcaaat tattaacagc ggtgaaccgg gtagcggagc agcagtaaaa
    18361 ataaggggag ttggctcaat tggcgattat gcaaaacccc tttacgtggt ggatggagtt
    18421 tttgttgaca acatcgactt tttaagctcg agcgatgtgg aagatcttac agtgctcaaa
    18481 gatgcctcgg ccgctgctat ttacggtgtt agagccgcaa acggtgtaat tattgttacc
    18541 accaggaaag gcaaaacagg tatacctact gtaagctacg atagctacgt tggattccaa
    18601 gttccagtaa acatccttaa aatggcttca aaggatcaat atattactct ccttaacgag
    18661 gcaaatgaga atgtcccggg ttatgttccc aaaaatcctg ccgattaccc tactagcacc
    18721 gattggtatc aggaacttgt tcgcccagct ggtatgaata atcataacct ggacatttct
    18781 ggatcaaccg ataagacttc atactcaatt gggggcaact atatatacca gaatggtatt
    18841 atggatgcca agaacaatta ccagcgttat aacatcagag gcagacttga ccagcaagta
    18901 aataaaaacc ttaaaatagg cattaatact attatatcca attactccag gttcaaccca
    18961 aatacgtatg ctttttttgg tgcatatgtt aacccacctg tttactcagt ttatgaccca
    19021 acaaacagcg atgcctaccc tgtaaagttt ggagctccgc aaaaatacgg atttggtaac
    19081 gagtacggga atcctgttgc gtctgcctac tataccgata actttgaaaa aggacataaa
    19141 gaggttttca gtatatatgc tgaactcgat attattaaag ataaactaac ctaccgtgca
    19201 gcctacaatc aggatcagga taattggaac caacgcagct atacccctca gttctatgtt
    19261 ggtggctcgc agggagtaac taagtcaagc ctgacaaaaa catttggagg tgcctccaaa
    19321 caaattattg ataacctgtt aacctataag aatagtgctg gaaaaacatc atactccata
    19381 ctcctagggc attcgatcag aatggagaaa tgggaaaccc tttcaggctt tgccaatagc
    19441 gttcccggat atgatgaaca atcgaaatac cttataaatg gctcattccg cgaccgtaat
    19501 gcatacgacg gggcagcaag gtacaacgga ctgtcatttt ttactcgagg tacactgaac
    19561 cacaatgata aatatcttgc aacccttaca ttccgtgccg atgcaagttc aaaatatcaa
    19621 caaaaatggg gttattttcc ctcgttgggt attgcatgga atatcaccca ggaaaacttt
    19681 agtagaatta atgaaaagtt tgccaacctg aagttacgct taagctgggg aatgcttggt
    19741 aatgacaatg tacccgccaa ctcctcgatg attcttggtc aaacaggtgt tggaagttca
    19801 ggagtgtttg gcgataattt ggtggatggt atgggtgctc aaactgttct gcagaattac
    19861 ctgaagtggg aaacagttac agagtttgat atgggttttg actttacact taaaaatcag
    19921 aaagtatcgg gtgaactcga ttattaccga cgtgttacca acaatgtagt gttttatgct
    19981 cccattgcca ccggaggtgg tattgctgag cttttaggta ataatggaaa agttttgaac
    20041 accggtgtgg agttctcgct gaactacaaa aacgagatat caaaggacct taattacaac
    20101 atttcgttca acgctacatt caaccataac gaggttcttg aacttaatgg tagggagtac
    20161 atccccagtg gttatgtaag ggggaactat acaacccgaa ctgctgttgg gcaccccata
    20221 ggctcattct acgggtatga gattgacggt gtatacaaga gtgaacttga agctttacgc
    20281 gacccagtaa gtcaagccat taaggataaa ggatatttca agtataagga tcaaaacggc
    20341 gataaggttg tggatgcaaa tgataaagtt tacctgggta gtgcaatacc ttggctaatt
    20401 acggggtttg attttggttt aaactacaaa aaatttgatg taagcctttc cattaccgga
    20461 cagtacggca acaaaatttt gaatgccaaa aggatgaaca gggatgtatt ttccgatgga
    20521 aactacgatg aagattttta caataaccga tggactccct caaataaatc gaccgattac
    20581 ccatcggctg aagcctataa ctcttcattt attcagcaag ctaacgattt ctttgttgaa
    20641 aatggttttt acattcggat tcagaatgtg caaataggct acacaaccga taaaattcct
    20701 tacgtaccca aatttcgggt attcttcaca gcccaacgcc catacacatt ctttacctac
    20761 aaaggtttta cccctgaagt tggaggttcg cctatcagta gcggcatcga cttcaatgtt
    20821 taccccatgc aagccattta tacactgggt attaagctga tattttaact caaaatctaa
    20881 gcgccatgaa aacattaaaa tacatcataa taactcttgc atcggttctg ttactttgga
    20941 actgtgaaga ttttctcgac tttcggcagg agggaacctt acccacaacc ggaattgatt
    21001 acaccaagtc cgaaaatata tttctttcag taagcgctgc ctacgccaag ttgagaaact
    21061 ggggagcaca tgtttttccg tacgttgggg cttttgaaat tgcctcggac aatgccgata
    21121 aaggtagtac acctgaagat aacccagaaa tgcgcgacat tgacaacctg aactttcagc
    21181 ctaacaacgg tcttataaat gccttgtgga ctggatacta cgatatagtt agcggtgcca
    21241 actacgccat tcatcaaatg ccccttttcg ttcaaaactt acagaataaa tcggatcgcg
    21301 attatgccat tcaatgccaa ggtgaggcta aagccattag ggcttacgca tactttaacc
    21361 tgacccgttt atttgggcga gtacctatag tagatacggt tctttcctct gaacagttag
    21421 cctcacttag tcaggcgagt acaagtgaat tatacgcttt cattgagaaa gatctggaag
    21481 aggccattgc tgttctaccc gatggttaca ccaaggaatg gggtggccga atatccaaat
    21541 acactgcaat ggcaataaag gctaaagttc acctatacca atcggaatgg gactccgtag
    21601 cgtcacttac cgataaaatt attgcatcgg gcaggttcag acttctcgat aacttccgcg
    21661 aagtatttag catggatgga gagaattcaa aggaatcgct ttttgaaatt caaagttcta
    21721 cacttggaaa gtcatcgggc gatgctgctt atcttgagta tgggtatgta caggggccgc
    21781 gcggaaactc acctgccaat atgcaaggct ggggtttctg tacaccaagt caaaacctaa
    21841 tcgattttta tacttcgcgt ggtgaaataa taagacccgc aactacactg ctttacaggg
    21901 gaactaaaac accggaaggc gacagcatta aaatgagttg cgccaaccct gtttacaacg
    21961 gaaaggtata tacaccctca tcgcaaaata gatgggtgta caatgggtat ggttttgacc
    22021 ataacatcag ggtaatcagg tattccgata tccttttgat gtatgccgag gcattggtgc
    22081 aaggcgctac cgttccctta acctgtggct taagtgccga taatgctatt aacatggttc
    22141 gaaacagagc tggtttaccc agtatcagcg gagctactct acaggacatt tgggacgaac
    22201 gccgtgccga actggccatg gaggaggaca ggtattttga tctggtgcgg acaaatcagg
    22261 cagcaaccgc acttgcatcg aagggatttg tggtgggaaa gcatgagcat tacccaatac
    22321 cggctgccca aatgcaactg aatccaaatt taactcagaa taacggatat taatcaaatt
    22381 aatcaattta aacctatgtc taacttaaat ttaagtccta tgaagaacaa attcagaatt
    22441 tttggtgcgc ttctttttgg cgctgtagta atgggtggat tactattcac cgcatgcagt
    22501 aaagatgatg atgacaatga caatgggaag gttgatccaa gtaccattgc aacagctaac
    22561 ctggttgctt acatgccatt tgacgacaat gcccttgaaa ccattggtaa tattacccca
    22621 tcgcaacagc ctaatgttac ttacgttgta ggtcgtaggg gtaaggctta ccagggtgcc
    22681 agtggcgcat atctacgcta caatctacct gacaacagca aacttaaaac cctaaagtct
    22741 tacactgttg ccatgtggat taaatcccct aaagtagatg gtacgcctgt acctaaagtt
    22801 atcgaaatcg gaaaatccga tgatttattc tggggaaacc tcacaatcat gattgagagg
    22861 cttactgatc ctggtgccga ttcactattc tttaagctac actttaaaaa agtaggtgtt
    22921 ccttgggaag gtcaatggat tgaagcaaag aaatcaattt tccaggtaaa taaatggatg
    22981 caccttgtgt tccagtacga cgcaactacc tccaagttta tgatttacaa ggatggtgca
    23041 aaagttgaat tccctgaagc agttgaaaat cgctacgcca acgatcagca ggaacccctt
    23101 ggcgatctga actttgttaa cgccgacgtg cttaacattg gcgcatggag accaaaaacc
    23161 gattctcaag ccactgatga atggatgggt tggttcatgg gcaacctgga tgaactccgt
    23221 gtttacgata aagcactatc agctacagaa gttaaggctt tgtacgatgc cgaagtatca
    23281 caaattactg aataaccgaa tttttagggt gggggaaaca ccccacccta ttttttatta
    23341 aaccctatga agtacgtttt ttcgataatc gcactaactg gaattttaat taccgtaacc
    23401 ttttgcaaag aagaggaaac ggtaaaaccc attggaaata taatggtcga ctcaattacc
    23461 attgacggga ttaaagttta tgacaatggt caaatcaacc atattggttt taaaccaaac
    23521 ataaaagtct atttcgataa acctgttgat acaacaaaaa tcgacaccta caatatttac
    23581 ttttctggag atacatacaa taattacacc catcaattca atactgattc aaaagtttta
    23641 agtattatac ccaacgaaaa tctgaaacct ctcacgcttt atcggttcac ggtatcgcag
    23701 ggcaaaaact tgggtggcaa tatcataaat gggtatacag ccaagttttt taccaagctc
    23761 gattcaaccg ataagtttca gcaaatttcg gacgatagcc tattaacgtt aatacaacat
    23821 cgaaccttta agtacttttg ggattttgcc cacccaacct caggtttagc aagggaaagg
    23881 aatacatcgg gcgatatagt tacaattgga ggtagtggtt ttggcataat ggcaatcatt
    23941 gttggcattg aacgaggttt cataaccaga gccgaaggca taagccgttt ctcaaccatt
    24001 gtaaatttcc ttaaagatgc cgataggttt catggcgcat ggggccattg gtataacggt
    24061 gttacaggta aagtatatcc tttcagtaca aacgataatg gaggcgactt ggttgaaacc
    24121 gcgtttctgg ttcaggggct tatcacactc cggcagtacc ttaaccctaa tgagagttct
    24181 gaaaacaact taataaacca aataaatgag ttgattaatg gagtagaatg gacatggtac
    24241 actaaggatg gcgaggatgt tctttactgg cactggtcgc ctaccgttgg ctgggcaata
    24301 aatatgcaaa ttagaggcta caatgaggca ttaatcgttt atgtattagc cgcttcttca
    24361 actacctacc ccattaaccc ttcggtttac cattccggtt gggcaaggaa tggagctatt
    24421 accaacggga agacttttta tggaatatcc ctaccggttg gttttgacta cggggggccc
    24481 cttttctttg cccattactc atttatgggc cttgacccca gaaacttaag tgataactat
    24541 gcaaactact ggcagcaaaa cgtaaaccac acccttatta accgtcaaca ttgcattgtt
    24601 aatccaggac gatacccctt gtacagcgct gattgctggg ggctaacagc atcggacgac
    24661 aatacaggct atggtgtgca tgagcccacc cgcgatattg gagttattag cccaactgct
    24721 gctctatcgt ccatgcctta tacccctgag gaatcgaaaa aagtggcacg cttcctctac
    24781 tataaacttg gaaacaaggt ttggggtgag tacggctttt acgatgcatt taatgtaact
    24841 gcaaattggt ggggtacaag ctatttggca attgaccagg gaccaatagt agtaatgatt
    24901 gagaactatc gtagcggttt atgctggaat cttttcatgt cggcacccga ggttaagcaa
    24961 ggactttccc gtcttggttt caatactagc aggtaaccta aaaaacgcat ggctatggct
    25021 atcaatggta aaattggaat gttttggaag gtatcaatgg tgcttacctt cctaatgctc
    25081 tcatgctcaa aaactaataa tatcagcttt ccatcaaagg gcaaaataaa ttactcctta
    25141 actgccgagg aagaaaaaat gctcgattca attcagtata agacttttca gtactttatg
    25201 aatgagcatc acccacattg gggtgctgta aaagacagag cagccaaatg gtcgccaatt
    25261 agcattgccg caacaggttt tgctattcct tgctttgcaa taggtgctga gcgtaaatgg
    25321 attagtcgcg atgaggcagc tcaaataacc ctaaatatcc ttaaattctt taaaaattct
    25381 gagcaaagtc ctgatcctct ttctacgggc tataaaggct tttactacca tttcctaaac
    25441 atggaaactg gaaaaaggga atggaagtgt gagttatcat cggtagatac aggcctactg
    25501 ctcatgggga taatttttgc ccgcaactac tataatgcaa acaatagcgt ggaacgtcag
    25561 atccgccaaa ttgctgactt tttaataaaa agggttgact gggatttcat gcaaatgcca
    25621 cctcaagggc aatacgctta caccatttca atgggatggt atccggaatc gagtatacac
    25681 aatatgggct ggaaagggta taacgaagcg ctattcctat acatactggc ggcaggatca
    25741 ggaatgaaaa atattgaaaa ggcatacaat tcatggctcg aatcctacaa ctggtacact
    25801 ccttacaagg atttttcaca tgttgcattc ccgccacttt ttggccatca attttcacat
    25861 gcttttatcg attttcgtgg tatacaagac tcatacatga gcaaaaaagg aatcgattac
    25921 ttcattaact cccgtattgc cacttacgct cagcgagagt attgcatcga taatccccat
    25981 gggtgggttg gttacgattc actttgctgg ggaataactg catgcgatgg gccaaccgat
    26041 aagtataatt ttgacaataa agtttttctc ggatacgccg gcaggggggc cagcggaagg
    26101 gaacttaact actttgatga tggtactata gccccctatg ggcatctatc atcgctacct
    26161 tttgctcctg aaattgtgct cccaacaact aaagcaatgt tgaagcgcta tggcgataaa
    26221 atatggggta aatatggatt ttacgactcc tttaatccaa ctgctaaatg ggttgataac
    26281 gactttattg gaattgatca aggcccaatg cttattatga ttgaaaactt cagaacaggc
    26341 atggtttgga actatataat gcccgatcca ataattcagt cagggttaaa gaaacttggt
    26401 tttcagaata taaattgatt gttttctgaa attacgaatc gtcagttttt ttcttattaa
    26461 aacactcttg gtatgaacca tcacttaaag gcaacaatta ctgctgcttt gctatcagtt
    26521 ttaacatttc acatcggagt aaaggctcaa acagtaactc tcgatcagtt cgaatcaact
    26581 gaaggctgga actatattaa gtcggatggg gtaaatatta ccgctcaaac cgataatggt
    26641 ctttccggca aatccatttg cataacatat aactttgtta agggtacagg ttactgtggt
    26701 attcaaaagc tttttcccat taatttgcca gagaactatg agataacctt ttacataaaa
    26761 gccgaatcgc cctcaaataa ttttgaaatt aagtttattg acagtactgg taacaatgtt
    26821 tggtgggtaa acaaccgcaa ttacaatttc cctacaagct ggcaaaaggt tagagtaaaa
    26881 cctcgccata tcagttttgc ttgggggcca accaccgata cacgttttag aagaattgat
    26941 agaatagagt ttaccgtggc atcgttcgtt ggaggaagtg gaaaaatatg gattgacagt
    27001 ctaaccttta aagccctacc cccaatttcc aatgagtacc ctgccccagt tgcctatgaa
    27061 atttcgggta cacaatcaaa accaattgcc gaaatacttg accagcagcc ccacagcgtt
    27121 tggattgcca aaaacaaaag tattcaggag atactgattg attttaaagc gctgcgtgag
    27181 attggcgggt taaaaattga ttggatgcaa ggcaaacaag cccaaaaatt cgatgtattg
    27241 ttatcctatg attccataaa ttgggaaaaa acttttactg tcaatttaaa tattaacagc
    27301 acaagctaca tccgcctgcc tgagtttgaa acccgattct taaaactaaa actaagccaa
    27361 agtaataatt ccaagggttt aggaattaag gatattgaaa tcataaaggt tgaggaatcg
    27421 aacacactta acaacttttt taggtacata gcaaagaatt cgccaaaggg ctattaccct
    27481 cggtactttc ttgatcaggc ttcctactgg acaatttcag gtgtaagtgg cgatgcaaag
    27541 gaggcactga ttaacgaaga tggcatggtt gaagttgaaa aagcaagttt ttccattgag
    27601 cccgttgtaa aggttggcaa taaaatttat gattggtcga atgtaaaaac ctctcaatcg
    27661 cttacctatt ttattgatgg taaaacctat gattacacac ctacggttaa ttggcatacc
    27721 gatgatattg acttacaggt aggcattacc tcctacggaa atgcaaataa ctcatcaaaa
    27781 ctactgattg gttacaccct aaaaaacaat accaacaaaa accaaacaat agatttttac
    27841 ctgctaataa gaccctttca ggttaaccca tactaccaga ccttaaacct tgccggtgga
    27901 gttggaacaa tcaaaaaaat atcattagac gcatcaaaag atagggttga agttgataac
    27961 acctcactat actttaccca aaagtttgag tcgttctttg ccggttcatt ctacagcgct
    28021 aatcccaccg aattaatacg gagtaaccac aaagaagtag taatggc
//
LOCUS       DAOOWJ010000026        26417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1840_length_26417_cov_9.057355, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000026 DAOOWJ010000000
VERSION     DAOOWJ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 26417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26417
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1840_length_26417_cov_9.057355"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..1291
                     /locus_tag="PLW22_04225"
     CDS             410..1291
                     /locus_tag="PLW22_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73945.1"
                     /translation="MKAQGIARVAIVITFAYLLVSCDGSSKKKIELLTKENEALRNEA
                     RIKDSTINQFFAFMGEIEQNLSVIKQKEQTISKNAIAGSELKPDVRQQIDEDIQTINM
                     LMDKNRQTIAALRKKLKEANLKVAEFERMIANVTQQLEEKEHEIEQLKENLTQLNFTV
                     TQLNARIDTLMEEKVEMSVKIQSQTEQLNTAWYAIGTRRELIDNKVIDKTGGFLGIGK
                     SSKMKAEFNQNYFVRIDITKTVSIPLSAKKAIVVTTHPKESYTLTTNEKGIIEELKIT
                     DTQKFWSVSKYLVIEVE"
     gene            1412..4090
                     /locus_tag="PLW22_04230"
     CDS             1412..4090
                     /locus_tag="PLW22_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73946.1"
                     /translation="MIKSSYTRLLLLLGIVLLLGVWSCSTQKNTFVSRNYHNLTSFYN
                     YYYNAYDSYKSGTYKAEKGINYNYTLPLPVLLIGESQVPGIVSSDMERTLNKCTMLLA
                     RHSITVKPARTKNTLSPKEKEFMSLNEYVKWARESWLLVGKAHIWKQDLTKARSTFEF
                     VIRQFPGLPIWFEAQVLLARVAILEGDFVDAQDRLKSVESNRKRPRNKEFNHLLSSTW
                     AYYYQKQQQPDAMLPHLRKAIELAPKRNDKIRYTYILAQTLQQVGKNGEALVAYQKVL
                     RLNPAYDMAFSARIQMIALSQKQGDELKRELIKLSKDAKNADYLDQLYFTLGNLEKKD
                     GNIDKAIEYYTLSAKYSKSNSNQKGMSYLVLADYYFSKNDYTRAQAYYDSSYNSLDNT
                     YPEFERIEQKAKYLNSLVENITTIKTEDSLLRVAAMPKEERDALIKAIIANIEVEEQR
                     ARAMEQEERNQSLLYQQTQRYRTENTQEGGKWYFYNQASLSYGQSEFQMKWGKRKLED
                     NWRRKNKRVLSSDEVTVAGTGNASGQEPQKQLSPKTPEYYLVNLPFSDSLKQLSVEKI
                     KNAMLRVAEIYEVNLSDYNEAKAAYLKLAQRFAEQDIAANAYYRLYRMASQYNRQDDA
                     ALFRSTLITNYPKSPYAILLSNPAYFEQLSKQQNEGEALYQKAYEFYTKGQLSEALSI
                     VQGGLAKTKNTPLEPKFMLLEALCQAKTAELKVFKTSLSNLAANFPKTEEGQLAEVML
                     KQVEQRELQLISGQTDLDIAPVTPQTEIQASIYSATDGEHMFVMLVPKRSNINQTKFN
                     IISFNVDEFLDADLNVANQPFTDYIEMITCTGLKDKQTAIKYFKMISKEPKVFAGLKP
                     TDYQMFVISIDNFARFLSDKSIPDYLNFFKANYKTE"
     gene            4095..5645
                     /locus_tag="PLW22_04235"
     CDS             4095..5645
                     /locus_tag="PLW22_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOU73947.1"
                     /translation="MQPTILWVDDEIDLLRPHIIFLEQKGYHVLTASNGHDALELVSQ
                     NPIDLVFLDENMPGIGGLETIPKIKAIKSALPVVMVTKSEEENIMDMAVGAQISDYLI
                     KPVNPKQVLLVVKKFIHGKELVTEKQLADYRNEFRLISDLISAAKTHLDWTEIYRKLA
                     IWQVKLSGNPDSNLKQMLEMQFEEANQVFGKFVKANYLQWFQTQGDKPLLSPSVLKSK
                     VFPIISSGSKVLLLVIDNLRYDQWKTIEPIIAESWQVSSEDTYFSILPTATQYARNAL
                     FSGLMPLEIHKTYPDLWVFDEEDEGKNLFEKQLLTAQLQRLGLNTNFTYIKTDTLKGI
                     AGGLNIKEFLGSNLAVLVYNFVDVMSHSRTDSEMMRGLAADESAYLSLTRSWFLHSDL
                     KGFLDQAASQGVQVVLTTDHGAIRVKNPVKVVGDRATSTNLRYKLGRSLNYNPKEVFE
                     IRKPEEAHLPKPNISSSFIFALGNDFLAYPNNFNYYASYYRDTFQHGGISLEEMIVPL
                     VTLNPIKR"
     gene            5647..6072
                     /gene="tsaE"
                     /locus_tag="PLW22_04240"
     CDS             5647..6072
                     /gene="tsaE"
                     /locus_tag="PLW22_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOU73948.1"
                     /translation="MTTIEIKSIQQIDEAAQRVLNAIHGKNIVCFYGTMGAGKTTLIR
                     AICKALGVADVVTSPSFALVNEYTDRNGKPVYHFDFYRIKRIDEVYDFGYEEYFYNPE
                     ALCLIEWPELIEQILPTQGVVNIQIAVNADGSRTLQISD"
     gene            6187..7401
                     /locus_tag="PLW22_04245"
     CDS             6187..7401
                     /locus_tag="PLW22_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOU73949.1"
                     /translation="MNSPKFSEFPHAKGLLVQEERIEYKKKYKQLIIGIPKEVDPTES
                     RIPLTPEAVEILVQNGHQIIIEKDAGRQANYSDMEYSEKGARIVATASEVYQSDIVLK
                     VAPLTPKEYEYLRENQSVLSSLHLNNHTGDYLKSLIQKRVTAIAFESISDADGEFPFV
                     RAMSSIAGSTSVLVAAEYLSNVNKGKGVMLGGLTGISPTEVVILGAGTAGEYAARAAL
                     GLGATVKIFDNSIKKLMDIQNILGQRLFTSIYHKQVLDKVLKTADVVIGTLAPDETNL
                     SYLVSEDQVRLMKRGAVIIDLGIDRGGCFETSEIRDHSNPSYVKHGVIHYCVPNITAR
                     VARTASIAMSNVFVPLMLQIAQSGGLLSQLKEDSGLRHGVYLFNGILTNEYLGKLYDI
                     PSRDINLLMAAF"
     gene            complement(7407..8183)
                     /locus_tag="PLW22_04250"
     CDS             complement(7407..8183)
                     /locus_tag="PLW22_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOU73950.1"
                     /translation="MPFAQKIEDAIERFYSAITVGVNHHKVGILSTIVVHLLLIVVFL
                     FLKIETRKEYYGSTIEMEFEEPKEEAIVQQKLEPTLPPDVLKPEYETEAIRNFAVDAS
                     KTDLNAGLSDEKNTDADELYREANQVYERMQQNRELYEQSQKDIEANIPNTPEKTVPK
                     SKEGQYKGPTVVSYYLLGRKALHLPVPSYKCELGGQVVVNIEVKPDGRVADAVIDRAN
                     SVNDDCINQAAIQAAMASIFTSVSGTARQRGSITYLFVPQ"
     gene            9059..9535
                     /locus_tag="PLW22_04255"
     CDS             9059..9535
                     /locus_tag="PLW22_04255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU73951.1"
                     /translation="MDCFTAIDFETAQGYRWSICQVGLVRVEHGKITNEVNILVQPPN
                     NYYWDRFIDIHGITPDDTAKAPTFDKVWHKIEPFIKEQTVVAHNGLSFDFPVLNKTLE
                     YYGMQAPDYEIYCTYRIFKDNLASLCNFYKFHLDHHDALSDARACAELFLIHLQNK"
     gene            9540..11234
                     /locus_tag="PLW22_04260"
     CDS             9540..11234
                     /locus_tag="PLW22_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOU73952.1"
                     /translation="MSCLTEKTIGEILENKFFIPSYQRGYRWTERQVEDLLNDVWDFI
                     TKTGKKDNEWYCLQPVVVKKTNNHYEVLDGQQRLTTIFLILKHLERFIESEKKSFEIE
                     YETRNTESSNSKLFLQSIDLKSEKESKENIDYFHIFQAYQKIKEWFQTKANQGYNSIS
                     SKFVTPFLESTKVIWYEVSSEKDAIEIFTRINMGKIPLTNAELIKALFLNSSNFPDSD
                     TEEIRLRQLEIASEWDRMEYSLQNDEFWYFINNSDNELPTRIEFLFNIMKNIDGVKNG
                     NDQYSTFRFFSEKFKSKSKDEIDTNWKEIKRIFQTIEEWFTDRELYHKIGFLISANSD
                     ITELLKEVKNKQKQEFRKLLDIKIAEKVKCENLEELEYGKHSDLIRKILLLHNIQTML
                     NNKNETIRFPFERYKKEKWDIEHIHAIATKVKVKFEDQNTWLFYNFVKTEQHTNLELN
                     SQIENAIKGNQIAEDDFQSIVEYVLGDDDNTIRNLCLLDRGTNRSYKNDSFKNKRNKI
                     IENEKNGTFIPVCTRNVFMKYYSKELKDLEIWNENDRADYLDDLKRVVYNFKSQVL"
     gene            11231..13399
                     /locus_tag="PLW22_04265"
     CDS             11231..13399
                     /locus_tag="PLW22_04265"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOU73953.1"
                     /translation="MKIERIEKIANNMKDIDGKVLANELVERLNSSSFWELLTEMTKI
                     QIPIIQRDFAQGRENEKTNKIRSLFLESIIEAVESEKGSLELDFVYGNVEKDVFQPLD
                     GQQRLTTLFLLHWYIALKTGELPNTKKHFIKFTYETRISSREFCNELVDNGEFLGIGE
                     KISEKIYDSKWFFLSWKKDPTIKAMLVMLDAIEERLLSKNDEELKIFWQKLTSENPPI
                     LFHFKQLNDIGLTDDLYIKMNARGKGLTDFENFKAKFEKHIKKNVFEKELELSENNKE
                     KWKELTEKTFSHRIDTVWTDLFWKHRGEDNIIDNEMIKFIAGIAINNYAQNLEILENS
                     EDELRTRKILEEKKEKNITDEAVKRERIERRITALFNNPADVSPYDFPTKESFEYLKK
                     CFDVYSKNQNDELLPANVTLWDFCKTKKVKINSDTEIDNNLFIEFIKKNETTYSQRVL
                     FYAQTQYLVNLENFDSESFSNWLRVVRNIVQNATIDSAYAFIGAIGLINELSSGCENI
                     YEFLSSTTLKSGFATKQIEEEKLKAALIKSDNNWKSQIFKAEDDIFLKGEIKFLLDFS
                     KVGEEYKIQEFTSISDKFLLLFESKDDLLRKALLTQDDYPVWDGRTSSLGWAHRYSLL
                     NTENEWKDAFRKKSDKFINAVYKLISNSFNLSDDRITILKNVIQNATPLNDYRSHLIK
                     NDSLLRDCYHKRICLNEKKTILYVLEKTRVVGENYKEIKV"
     gene            13459..15129
                     /locus_tag="PLW22_04270"
     CDS             13459..15129
                     /locus_tag="PLW22_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73954.1"
                     /translation="MEQNIKFRLLESIGKVYEQSKDCKLDSAFFEKVDVELKHLSDYF
                     KVSRNQSLIVAMVFALNYKGDTVDLKDLIEYFDCNPMKLLEFSDDFDVLYSKGIFKKQ
                     KSSHRVRLALSNDQFTINEKITEAILNNKPMPVIEREGFKDVIELLEKFHKLGEQRDS
                     EELSTFELFAQAKELISSNLHFPLIKKINDYQFSIEDTYLLLYLIWETITGNETTDIG
                     RATESIFDNPSERVKYVQKIMSNENKLISQNHLEIVEANFFSYTEMKLSESSVKMLQE
                     LGLKLFANIKKRDNIIEPAKINSKTLYFNEFERKQLDLLKTLLQEDNFKKTQNRLQSK
                     GLPKGITALLHGSPGTGKTETVFQVAKDTNREIVKVDISQSKSMWFGESEKIIKRIFT
                     DYKAYAKDCERMPILLFNEADAIITKRKENSTSNVSQTENAIQNIILEELENFEGIFF
                     ATTNLAKNLDSAFERRFLFKIEFQKPDISVKAKIWNSKLPSLSESECEILANRFDFSG
                     GQIDNIVRKVEIHEIIHGITVDFNSILDFCKTELLSKNRGVRIGFTKA"
     gene            15135..16064
                     /locus_tag="PLW22_04275"
     CDS             15135..16064
                     /locus_tag="PLW22_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOU73955.1"
                     /translation="MSKRESIARYNLIIKKLRKQPSSFSEISDYLAFESDLQEYNFNV
                     SKRTFQRDIEDIRSIYNIDIQYDFSKKVYYIDFDEQPEVNERILEAFDTFNALNISDR
                     LSNYIHFEKRRPQGTGNLYSLLHAIKNKVQISFSYQKFWENTSTQRVVEPYALKEFKS
                     RWYLLARDLKDKKVKSFALDRLTQLVITKKQFVYPIDFDVNEYYRHCFGIIGPNAKEP
                     QEVILTFSRFQGNYIKSLPLHETQQIEFENENELRIKLKIFITHDFIMELLSFGENVK
                     VIKPESLVNSIKEVYNKALNLYKSNQNEKGKSD"
     gene            16671..17945
                     /locus_tag="PLW22_04280"
     CDS             16671..17945
                     /locus_tag="PLW22_04280"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOU73956.1"
                     /translation="MYELENESFRGRSKNGLKGAFTRDRKLTINNLIILVINFKSSIQ
                     RELDSFFKAIAKSDFKIREVTKGAFSQARAKLNPWAFQRLNEVAVNAFYEKANYDVWG
                     GLRVVAVDGTRLVLPNHPSVIEEFGQHQFGPKADSPRSLAMGSMLYDVLNQITIDAQL
                     APYASSERDLLMKHLGHVEEGDLLLLDRGYPCFWLLFLLKARKIEFCVRLKDDWWLQV
                     KDFTRSDEKERLVTFSLPKKDVKKLSEYPEMLGQTITCRLVKVELENGDIEILCTSLL
                     DDQYYTHKDIEELYHYRWSEEEAYKLLKSRVEVEAFSGKTATAVKQDFYAKVFLMTLC
                     AAYAHPIEERVTAEYKADKERKHDQKINRTNAISMTQDILIGVFIRKQFKAALEAFDD
                     IVQNTREIIRPNRKVPRNKKPKRLYYMNYKRL"
     gene            complement(18608..19855)
                     /locus_tag="PLW22_04285"
     CDS             complement(18608..19855)
                     /locus_tag="PLW22_04285"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOU73957.1"
                     /translation="MFNTNNEQRTTNNLLSFRADFPILSREVYGRPLVYFDNGATTQK
                     PLCVLQTIERFYREINANIHRGVHKLSEESTREYENAREVVRKFLNARKTSEIIFTSG
                     TTASINLVAYSFGEVFVRQGDEVLVTEMEHHSNIVPWQLLCERKGATLKVLPFDSNGE
                     LMLNKLPELLTPKTRIVAVTQVSNTLGTVNPIAEIVRIAHSQNIPVLVDGAQGVKHGK
                     IDVQQLDADFYAFSGHKIYGPTGIGVLYGKEEWLERMVPWQGGGDMIATVSFAKTTFN
                     ELPFKFEAGTANYIGAAGLATALNYYSSIGPDIATAYEDELLKYATTKLKEIDGLTIY
                     GNAPEKAAIISFLLNGIHPYDTGMILDKMGIAVRTGNHCTQPVIDHFGIDGTVRASLA
                     FYNTFEEVDRLVEALHKVKEMFG"
     gene            complement(19860..20213)
                     /locus_tag="PLW22_04290"
     CDS             complement(19860..20213)
                     /locus_tag="PLW22_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU73958.1"
                     /translation="MDYKELDVWKSSIELAKLVYHFTRQLPSNEEYGLSHQMRRAAVS
                     IPSNIAEGIGRNTSKETIQFLFIARGSIFELETQLILCKEIFKVDTHDYDALFQKLQN
                     CKMLINGFINYYQKK"
     gene            complement(20310..21677)
                     /gene="sufD"
                     /locus_tag="PLW22_04295"
     CDS             complement(20310..21677)
                     /gene="sufD"
                     /locus_tag="PLW22_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOU73959.1"
                     /translation="MSDKTIATPILEELVSLYQNNLKLITEGSADILNRHREQAMQHF
                     RELGIPSQKNEKYKYSRIEPLFAHEYQKHFAPKRIAFNVDDIFRCDIPELDTHLALVL
                     NGFYLPKGEKLTTLPNGVIFGSLAEAAIKFPNLVNGRYNSLAENKTDGLVALNTAFAQ
                     DGVFIYIPRNVVESKPIQIINLLMSDVNQLVQYRNLIVVEEGAQANVLICDHTLSPKR
                     FLSNVVTEIFVGNNAQLEFVKMQNEHNDAAQLTHTYAHQQADSRLVTNVLSLHGGFIR
                     NNLAVTLAGEGADAQLYGLHLTDRTQHTDNFTFIDHAVPNCTSNELYKGILDDQATGA
                     FNGKIMVRRDAQKTQAYQSNNNLLLTADARMNTKPQLEIYADDVKCSHGATVGQLDNE
                     ALFYMRARGIGEREAKLLLMFGFAHEVVKRISIDTLRERIDDMVNKRLRGELSRCHNC
                     PMHCC"
     gene            complement(21705..22457)
                     /gene="sufC"
                     /locus_tag="PLW22_04300"
     CDS             complement(21705..22457)
                     /gene="sufC"
                     /locus_tag="PLW22_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOU73960.1"
                     /translation="MLTIKDLHAKINDKEILKGINLEVKAGEVHAIMGPNGSGKSTLA
                     SVLTGRELVEVTHGSVTFNGKDLLEMSPEVRAREGIFLSFQYPVEIPGVSMVNFMKAA
                     VNEHRKYRGLEPLSASDFLKMMRERKELVEIDSALTNRSVNEGFSGGEKKKNEIFQMA
                     MLEPKLAILDETDSGLDIDALRVVANGVNKLKRPDNAVIVITHYQRLLDYIVPDYVHI
                     LYNGRIVKSAGKELALELEEKGYDWIKNGENA"
     gene            complement(22528..22938)
                     /locus_tag="PLW22_04305"
     CDS             complement(22528..22938)
                     /locus_tag="PLW22_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU73961.1"
                     /translation="MNSSYKSSVFTDLIVWQKAHKLTLLVYSITQKFPHEEIYSLTSQ
                     FRRSVISIPANIAEGYKRKGRLDKLKFLNYAQGSLEETRYYTILSKDLGYIDLSTFEL
                     LDESINEVSFLLNSYSSTIAKNDDKSSTTSSNFL"
     gene            complement(23034..24482)
                     /gene="sufB"
                     /locus_tag="PLW22_04310"
     CDS             complement(23034..24482)
                     /gene="sufB"
                     /locus_tag="PLW22_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOU73962.1"
                     /translation="MAEEQDKILEEVTGSEYKYGFVTNVETETIPKGLSEDIIRMISE
                     KKGEPDWMLEFRLKAFRHWQTMEMPKWAHLEIPEINYQDIIYYAAPKQNAKLNSLDDV
                     DPEIKATFDKLGIPLDEQKVLAGVAVDAVMDSTSVKTTFRETLAEQGIIFCSMSEAIR
                     EHPDLVRKYLASVVPVTDNYFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINAANT
                     GQFERTLIVADDDSYVSYLEGCTAPMRDENQLHAAVVEIVALENAEVKYSTVQNWYPG
                     DKDGKGGIYNFVTKRGICKGRNSKISWTQVETGSAITWKYPSCILLGDNSYGEFYSVA
                     VTNNRQQADTGTKMIHIGKNTRSRIVSKGISAGHSNNSYRGLVKVLKGAENARNFSQC
                     DSLLLGDKCGAHTFPYLEVDNPTAVVEHEATTSKIGEDQIFYCNQRGLSTEDAVGLII
                     NGYAKEVLNKLPMEFAVEAQKLLQISLEGSVG"
     gene            complement(24713..24961)
                     /locus_tag="PLW22_04315"
     CDS             complement(24713..24961)
                     /locus_tag="PLW22_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOU73963.1"
                     /translation="MSKEQVVAKVEKAIEVIRPFLQNDGGDISLVEVTDDYIVKVKLH
                     GACGSCPYSIMTLKNGVEQAIIRDVPEIKEVVAVNLNF"
     gene            complement(24982..26088)
                     /locus_tag="PLW22_04320"
     CDS             complement(24982..26088)
                     /locus_tag="PLW22_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOU73964.1"
                     /translation="MVLSLDSVNEVLSTVLHPETSKDIVSMGMVQGLNVEGSKVSFTL
                     KLTKSNDPFASSLKKAATKAIQQKFGENVEVDILVVADVAPKVSPLKQKASISKVKNI
                     IAIASGKGGVGKSTIAANLAVAVAKTGARVGLIDADIYGPSIPKMFNMEHTQPEIVKE
                     EGMELIVPVENYGVKLLSIGFFVDPNSALVWRGPMATSALKQLIHQGAWGELDYLFID
                     MPPGTSDIHLTLVQEVPVTGAVIVSTPQDVALADAVKGISMFTSPSINVPVLGLVENM
                     AWFTPEELPNNKYYIFGKEGCKKLADRMKIPLLGQIPIVQSIREGGDYGEPVAIHASI
                     TGKAFDELAKNLIERVEWRNRELPPTQIVNVQHK"
BASE COUNT         8380 a         5253 c         5400 g         7384 t
ORIGIN      
        1 gcccctcctt ttgtcatgtt gtgcaaagtg aaggatataa ttcttagtgt acagtatgca
       61 gtgtacagtt gggtggtgtt ttgtgtttcg tattttgttt ttcgtgttta gataatcata
      121 tactgttaaa cctgccaaat ctgcgtaatc tgagttccat tccatctcac gaacaactaa
      181 tcctatcatt ccgagctaac cgaggaattt caattaataa agagttgtag gttcaatgag
      241 atgcttcgac tgcgctcagc atgacaaata ccaaaaaact ttagcagaac agtagtgatt
      301 cataaaaatt ttatgctata cagtttattc ccaaaattcc tgctccctaa aactcaacta
      361 aacaaagttt tacctaaatt gcacacttca aaccaattat tttacacata tgaaagcaca
      421 gggtatagca agagtagcta ttgtaattac gtttgcctat ttgctggtat cgtgcgatgg
      481 ttcaagcaaa aaaaagattg aactactaac caaggaaaac gaagccttac gcaacgaggc
      541 aagaattaaa gattctacca ttaatcagtt tttcgccttt atgggtgaga ttgagcaaaa
      601 cctgagcgtt attaagcaga aggagcaaac cattagcaag aatgctattg ctggtagtga
      661 gctaaagccc gatgtgcgcc aacaaattga tgaggatatc caaaccatca acatgctgat
      721 ggataagaac cgccaaacca ttgcggcact acgaaaaaag ctgaaagaag ctaaccttaa
      781 ggtggctgag tttgagcgaa tgattgccaa tgttacccaa cagcttgagg aaaaggaaca
      841 tgaaattgaa cagcttaagg aaaatcttac ccaacttaac tttacggtaa cccagctaaa
      901 tgcgcgcatc gatacgctaa tggaggaaaa ggttgagatg agtgtaaaaa ttcaaagcca
      961 aaccgaacag ctcaacacag cctggtacgc cattggaaca aggcgggaac tgattgataa
     1021 taaagttatc gacaaaaccg gaggtttcct agggatcggt aaatcgagca aaatgaaagc
     1081 tgaatttaac cagaactatt ttgtacgtat cgatattact aaaaccgtga gcatccccct
     1141 ctcggctaaa aaagcgattg ttgttacaac ccatcccaag gagtcgtata cccttacaac
     1201 caacgagaag ggaataattg aagagcttaa aattaccgat acccaaaaat tctggtctgt
     1261 ttcaaagtat ttggtaattg aggtagagta gcgtttttcc gttgaagaag gtaaaggaat
     1321 taaacccaaa gtccacaatt atttgtaata ttgtgcccct gtatactttt tggggctatt
     1381 atccgttttt gtaatgcaaa attgttttga gttgataaaa tctagttaca cccgattgct
     1441 gctacttctg ggtattgttt tactattagg ggtatggtcg tgctcaacac aaaaaaatac
     1501 gtttgtgagc cgaaactacc ataatctaac ctcattttac aattactact acaacgccta
     1561 cgatagctac aaaagcggta cctataaagc cgaaaaaggg ataaactaca attacaccct
     1621 gccattacct gttttgctga taggcgaaag ccaagtgccc ggaattgtat caagcgatat
     1681 ggagcgcact ctcaacaagt gtaccatgct actggcgcgg cactcaataa ccgtaaaacc
     1741 ggcacgaacc aagaacaccc taagccctaa agaaaaggaa ttcatgagcc ttaacgagta
     1801 tgttaaatgg gcaagggaat cgtggctgct ggttggtaaa gctcatatat ggaaacagga
     1861 cttgactaaa gcccgctcaa catttgagtt tgtaatcagg cagtttccgg gattacccat
     1921 atggtttgag gcacaggttt tgttggcacg agttgcaatt ctggagggcg attttgttga
     1981 tgcgcaggat aggctaaaat cggttgaaag caaccgtaag cgccctcgta acaaggagtt
     2041 taaccatctt ctttcatcaa catgggctta ctactaccaa aagcagcagc aacccgatgc
     2101 aatgctccca caccttagga aagctattga gctcgccccc aaaaggaacg ataaaattag
     2161 gtacacctac attcttgccc aaaccctaca gcaggttggg aaaaacggcg aagccctggt
     2221 tgcataccaa aaagttctga ggttgaaccc cgcctacgat atggccttta gcgcacgcat
     2281 ccaaatgatt gcacttagcc aaaaacaggg cgacgagctc aagcgtgagc ttatcaagct
     2341 ttccaaggac gctaaaaacg ccgattacct tgaccagctc tatttcactc ttgggaatct
     2401 ggagaaaaaa gatggcaata tcgataaagc aattgagtac tataccctat cagcaaagta
     2461 tagcaaaagc aatagcaacc aaaaggggat gtcgtacctg gtgctggccg actactactt
     2521 ttcaaagaac gattacaccc gcgcccaggc ctactacgat agctcataca actcgttgga
     2581 taatacctac cccgagtttg aacgaattga gcagaaagca aaatacctca actcactagt
     2641 tgaaaacatt accaccatta aaaccgagga tagcctatta agggttgcag ctatgcccaa
     2701 ggaggagcgc gatgccctta tcaaagccat tatagccaac attgaagttg aggaacagcg
     2761 agctcgcgca atggagcagg aagaaaggaa ccaatccctg ctttaccagc aaacccagcg
     2821 ataccgaacc gagaacaccc aggagggtgg caaatggtat ttctacaacc aggcatcgct
     2881 aagctatgga caatcggagt ttcagatgaa gtggggcaag cgtaagcttg aggacaactg
     2941 gcgccgaaaa aacaaacggg tgctcagcag cgacgaggtt acagtggctg gaaccggcaa
     3001 cgcctcagga caagagcccc aaaagcagct atcgcctaaa acacccgagt actacctggt
     3061 taacctacct ttcagcgact cgctgaaaca gctctccgtt gagaaaatta aaaacgccat
     3121 gctcagggtt gctgaaattt acgaggttaa cctttccgat tacaacgagg ctaaagcagc
     3181 atacctaaaa cttgcgcaac gatttgctga acaggatatt gctgcaaatg catactacag
     3241 gctataccgt atggcatcgc agtataaccg gcaggacgat gcagcccttt tccggagtac
     3301 gcttattaca aattacccca aaagccctta tgcaatactc ctctcaaacc cggcctattt
     3361 tgagcagctg agcaagcagc agaatgaggg cgaagccctt taccaaaaag cgtatgagtt
     3421 ttacaccaag ggccaactct ccgaggccct aagcatcgta caagggggct tagcaaaaac
     3481 taagaacact ccccttgagc caaagtttat gctgcttgaa gcgctttgcc aggcaaaaac
     3541 tgcggaactt aaggtgttca aaacctctct ttccaatctg gctgcaaact tccccaaaac
     3601 agaggagggt cagttagctg aagtgatgct gaaacaggtt gagcagcgcg agctgcagct
     3661 catttccggg caaaccgatt tggatattgc tccggtaacc ccgcaaaccg aaatacaagc
     3721 atcaatttat tcagctaccg atggcgagca catgtttgtg atgctggttc ccaaacgctc
     3781 caacatcaac caaaccaagt tcaacatcat ctcattcaat gttgatgaat tccttgatgc
     3841 cgacctcaat gtagccaatc aaccctttac cgattacatt gagatgatta cgtgtacggg
     3901 tctgaaggat aaacaaacgg ccataaagta ctttaagatg ataagcaagg agccaaaagt
     3961 gtttgcaggc cttaaaccca ccgattacca aatgtttgtg ataagtatcg ataattttgc
     4021 acgctttttg agcgataaat cgatacccga ctacctgaac ttctttaagg ctaactataa
     4081 aacagagtag aacaatgcag cccaccattc tatgggtaga tgatgagatt gatttgctcc
     4141 gtccgcatat catttttctg gagcaaaagg gctaccatgt tcttaccgcc agcaacggac
     4201 acgatgcact tgaactggta agccagaacc ctatcgattt ggtgttcctg gacgagaata
     4261 tgcccggtat tggagggctt gaaaccatcc caaaaattaa ggcgataaaa tcggcgctgc
     4321 ctgtggttat ggttaccaaa agcgaggagg agaatatcat ggacatggcg gtgggtgctc
     4381 aaatatccga ctaccttatt aaacccgtta accccaagca ggtactgctg gttgtaaaaa
     4441 agtttattca tggcaaggag ctggtaaccg aaaagcagct tgccgactac cgaaatgagt
     4501 ttaggttgat atcagactta atttcagccg ctaaaaccca cttggactgg acagaaatat
     4561 accgaaagct tgccatttgg caggtaaagc tatcgggcaa tccggactca aacctcaagc
     4621 agatgctcga gatgcagttc gaggaagcca atcaggtttt tggtaaattt gttaaggcca
     4681 actacctgca atggttccaa acccaaggag acaaaccctt gctctcacca tcggttctga
     4741 aaagcaaagt ttttcccatt atcagctctg gtagcaaggt tctgctactg gtaattgata
     4801 acctccgcta cgaccagtgg aaaaccattg aacccataat tgccgaaagc tggcaggtta
     4861 gcagtgagga tacctatttc agcatactcc ctactgccac gcagtacgcc cgcaatgcgc
     4921 ttttttcagg cttgatgcca ctcgaaatcc ataaaaccta ccccgattta tgggttttcg
     4981 acgaggagga tgaaggtaaa aacctttttg agaaacagct acttactgca cagcttcaga
     5041 ggcttggcct aaacacaaac ttcacgtaca ttaaaaccga taccctgaag ggtattgccg
     5101 gaggcctaaa catcaaggag tttttaggca gcaaccttgc ggtgctggtt tacaactttg
     5161 tggacgtaat gtcgcactcc cgaaccgatt ccgagatgat gcgcgggttg gccgccgatg
     5221 aatcggctta cctatcgcta acccgctcgt ggttcctgca ctccgaccta aaggggtttt
     5281 tggaccaggc cgcaagtcaa ggcgtacagg tagtactcac aactgaccat ggagcaattc
     5341 gcgtaaaaaa tccggtgaag gtagttggcg atagagccac atcaaccaac ctccgctata
     5401 agctgggacg aagcttaaac tacaacccca aagaagtttt tgagattcgc aagcccgagg
     5461 aggcgcatct gcccaaaccc aacattagct cctcgtttat ttttgctctg ggaaacgatt
     5521 ttttggccta ccccaataac tttaactact acgcatcgta ctaccgcgac acatttcagc
     5581 atggaggcat atcgcttgag gagatgatag ttccgcttgt aacccttaac cccataaaga
     5641 ggtaacatga ccaccattga aattaaaagc atacagcaga ttgacgaggc agcccaaagg
     5701 gtattaaacg ctattcatgg taaaaacata gtttgctttt acggcacaat gggagccggc
     5761 aaaactaccc ttataagggc aatctgtaag gcgcttggcg ttgccgatgt ggttacaagc
     5821 ccatcgtttg cgctggttaa tgagtatacc gaccgaaacg gcaagcccgt ttaccatttc
     5881 gacttttacc gcattaaacg tattgatgag gtttacgatt ttggttacga ggagtacttc
     5941 tacaaccccg aagccctatg tctgattgaa tggccggaac tcattgagca gattctccca
     6001 acccaagggg ttgtaaacat ccaaattgct gtgaatgccg acggaagccg cacattacaa
     6061 atatcggatt agctataacc ttaaaccttt gtaaatcgta aaaatcttac atattgcttt
     6121 tataccctaa caggtttgtg cataagctaa tatgtgctaa cttgcatttt taaaaaacta
     6181 acgggcatga acagtccaaa attttccgag tttccccatg caaagggatt gctagttcag
     6241 gaggaacgca ttgaatataa gaagaagtac aagcaactta tcattggaat acccaaagag
     6301 gttgacccca ccgagagtcg aattccgtta acgcccgaag ccgttgagat acttgtacag
     6361 aacgggcatc aaatcatcat tgaaaaggat gccggtaggc aggctaacta ctccgacatg
     6421 gagtacagcg agaaaggtgc acgcatagta gccacggcat cggaggttta ccaaagcgat
     6481 attgtactca aggttgcacc ccttacccct aaggagtatg aatacctgcg cgaaaaccaa
     6541 tcggtactct catcgctaca cctgaacaac cacaccggcg actacctcaa aagccttatc
     6601 cagaagcgtg ttacggccat tgcctttgag agcatatccg atgctgacgg cgaattcccc
     6661 tttgttcggg ccatgagctc cattgccggg agtacatcgg tgctggttgc tgccgagtac
     6721 cttagcaacg tgaataaagg gaaaggtgtt atgctaggtg ggctaaccgg aatatcgccc
     6781 actgaggtgg ttattctggg agcaggcact gccggggagt atgctgcacg tgcagcgctt
     6841 ggactaggag ccactgtaaa aatattcgac aactccatca aaaaactgat ggatattcaa
     6901 aatatactag gacaacgcct tttcacctca atatatcaca agcaggtgct tgacaaggtt
     6961 ctgaaaaccg ccgatgttgt aattggaacc ttagcccccg atgaaaccaa cctaagctac
     7021 ctggtatcgg aggaccaggt taggttaatg aaacgtggtg ctgtaattat tgacctagga
     7081 attgaccgtg ggggatgctt tgaaacctcg gagatacgcg accatagcaa cccctcgtat
     7141 gttaagcatg gcgtaatcca ctactgtgtg ccaaacatca ctgcccgtgt ggcccgaacc
     7201 gcctcaattg ccatgagtaa cgtttttgtt cccctaatgc ttcaaattgc acagagtggc
     7261 ggtttgcttt cgcagctcaa ggaggatagc ggcctacgcc atggagttta cctgtttaac
     7321 ggtattctta caaacgagta tctgggcaag ctttacgata tcccctcgcg cgatatcaac
     7381 ctgcttatgg ctgcctttta ggaacattac tggggaacaa aaaggtaggt aatactaccc
     7441 cgctggcgcg cagttcccga aacggaagta aaaatacttg ccatggctgc ctgaatagcc
     7501 gcctggttaa tgcagtcgtc gtttaccgag ttggccctgt cgataaccgc atcggctacc
     7561 cgtccgtcgg gctttacctc aatgttaacc acaacctgcc cgccaagctc gcatttatac
     7621 gaagggacag gcaggtgcaa ggccttacgg cctaagaggt agtacgaaac cacggttggc
     7681 cccttatact ggccctcctt cgattttggg actgtctttt cgggtgtatt gggtatgttt
     7741 gcctcaatat ccttttgcga ctgctcgtaa agctcacggt tctgctgcat gcgctcgtac
     7801 acctgattgg cctcgcggta aagctcatcg gcatcggtat tcttttcgtc gctcaggcca
     7861 gcgttcaaat cggtttttga ggcatcaaca gcaaagtttc gtatagcctc agtctcatac
     7921 tccggtttta gcacatcggg cggaagcgta ggctcaagct tttgctgaac aatagcctcc
     7981 tccttaggct cctcaaactc catctcgatg gttgagccgt agtactcctt gcgggtttca
     8041 atttttagaa acaaaaaaac aacaatcaaa agcaggtgca caacaatagt gctcagtatc
     8101 ccaaccttat ggtggttaac ccccaccgtt atggccgagt agaaccgttc aatggcatcc
     8161 tctattttct gggcaaaggg cattatggag gcagctatac tatttacctc acaaatatag
     8221 tgtcttagcg ggaaaattgg ttagcgataa tgaaatttag atagtatgtt tgcgtttagc
     8281 gatagtttaa ggttctagta agggaatatg ctatttatta aattgtttgt gatttgccat
     8341 gttgagcagc ctaacccgat aaagtttcac tgaatgcaca gtatggcaaa ccactattgc
     8401 ttgagcaatg gtagtaaatc aactgaaata catacatata cctggtaata gtatgggaaa
     8461 attaatgtcc ttttgataat ttgaaatgat tctttaattt agcaattagt atgattctta
     8521 tcatacatat aaacgagtta gcaacaagcc gaaaaaaaac gacacagcag taattttgct
     8581 gcaaaaagac aagacaatgg aaacagccga cgcacaaaac agcacatttg caaatcgcac
     8641 aagcagactc acgaccaaaa tttgcaaaag agttgttttt tccaccgcac gaagaaaatt
     8701 gcaacagtac gacatggatt ggctgacttg aaatttaatt ttgaccctaa ataaggaatg
     8761 ccgaaaactt ttagagtagg cgtaaattaa aactaaaaaa cttatggcta atttttattg
     8821 cgagtattgc gtaactaaat catcaagcgt ttcaagctta acagcaagtt catgtccacg
     8881 acacccatgg accaggtaaa ggaaaacaca aactttatga aggtggagaa aaatcacaat
     8941 atgtttgtaa gtattgtggc acaaaatcat caagtatttc cagcttaaca gctagtagtt
     9001 gttctcgtca tccaaatgga gcaggaaaag gaaaacatgc accagcatta taaataatat
     9061 ggattgtttt actgctattg attttgaaac tgcacaaggt taccgttgga gtatttgtca
     9121 agttggactt gttagagttg agcatgggaa aataacaaat gaagtaaaca tattggtaca
     9181 accacccaac aattactatt gggataggtt tattgatatt catggaatta cacctgatga
     9241 taccgccaaa gcaccgactt ttgataaagt ttggcataaa atagaaccat ttattaaaga
     9301 gcaaaccgtt gttgcccata acggtttgtc ttttgatttt ccagtattaa ataaaactct
     9361 tgaatattac ggaatgcaag cgcctgatta tgaaatatat tgcacatacc gtatttttaa
     9421 agataatctt gcatctttat gcaattttta taaatttcat ttagaccatc atgatgcttt
     9481 gagtgatgca agagcttgtg cggaattgtt tttaatccat ttacaaaata aatagatata
     9541 tgagttgttt aacagaaaag acaatcggag aaattttaga aaataaattc tttattcctt
     9601 catatcaaag aggttatcga tggactgaaa gacaggttga agatttatta aatgatgtat
     9661 gggattttat tacaaaaaca ggaaaaaagg ataacgaatg gtattgcttg caacctgtgg
     9721 ttgtgaaaaa aacaaataat cattacgaag ttttagacgg tcaacaaaga ttgacaacaa
     9781 tatttttgat tttaaagcac ttggaacgtt tcatagaaag tgaaaagaaa agttttgaaa
     9841 tagaatatga aacaaggaat acagaaagct ctaacagcaa actatttcta caaagcattg
     9901 atttaaagag cgaaaaggaa tctaaagaaa atattgatta ctttcatatc tttcaagcat
     9961 atcaaaaaat taaagaatgg tttcaaacaa aggcaaatca aggttataat tcaataagtt
    10021 ctaagtttgt tactccattt ctagaaagca cgaaagttat ttggtatgaa gtttcgtccg
    10081 aaaaagatgc aattgaaatc tttacccgta ttaatatggg gaaaatccca ttaacaaacg
    10141 cagaattaat aaaagccctt tttcttaata gttcaaactt tcccgattcc gatactgaag
    10201 aaataagatt aagacaatta gaaattgcat ctgagtggga cagaatggaa tactcattgc
    10261 aaaatgatga gttttggtat tttataaata attcagataa tgagctacca actcgtattg
    10321 agttcctttt caacattatg aaaaatattg atggtgtaaa aaacggcaac gaccaatatt
    10381 caacatttcg tttcttttca gaaaaattca aatcaaaaag caaagatgag attgatacaa
    10441 actggaaaga aattaaaagg atatttcaaa ctattgaaga atggtttaca gaccgagaac
    10501 tatatcataa aatagggttt ttaatttcag caaattcaga tataacagaa ttgctaaaag
    10561 aggtgaaaaa caaacaaaaa caagaattta gaaaactgct tgatatcaaa attgcagaaa
    10621 aagttaaatg tgagaatttg gaagaactgg aatatggaaa gcattcggat ttgattcgaa
    10681 aaattttatt actgcataat atacaaacaa tgttgaataa taaaaatgaa actattcggt
    10741 ttccttttga acgatataaa aaagaaaaat gggatattga acatatccat gcaattgcaa
    10801 ctaaggttaa agtaaaattt gaagaccaaa atacgtggtt attttacaac tttgttaaaa
    10861 cagaacaaca tactaattta gagcttaatt cacagataga gaacgcaatt aaaggaaatc
    10921 aaattgcaga agatgatttt cagtcaattg tcgaatatgt tttaggtgat gatgataata
    10981 ccattcgcaa cctatgtttg cttgatagag gtacaaaccg ttcttataaa aatgattctt
    11041 tcaaaaataa aaggaataaa ataattgaga atgaaaaaaa cggaacgttt attcctgttt
    11101 gtacaagaaa tgtgtttatg aaatattatt caaaagaact gaaggatttg gaaatatgga
    11161 atgaaaacga ccgagctgat tatcttgatg atttaaaaag agttgtttac aattttaaaa
    11221 gccaagttct atgaaaattg aaagaatcga aaaaattgca aataatatga aagatattga
    11281 cggtaaggtt ttagccaatg aattggtaga acgtttaaat tcctcatcgt tttgggaatt
    11341 acttaccgaa atgaccaaga ttcagattcc gataattcaa cgtgattttg cacaaggtag
    11401 ggaaaatgag aaaaccaata aaataaggtc tttattctta gaaagcataa ttgaagcagt
    11461 tgaatctgaa aaaggttcgc ttgaattgga ttttgtttat gggaatgttg aaaaagatgt
    11521 ttttcagcca ttagacgggc aacaacgttt aacaacttta ttcctgcttc actggtatat
    11581 tgctctaaaa actggtgaat tgccgaacac gaaaaagcat tttataaagt ttacttacga
    11641 gacaagaata tcatctagag agttttgtaa tgagcttgtt gataatggag agtttttggg
    11701 catcggggaa aaaataagtg aaaaaattta cgattcaaaa tggttctttt tgtcgtggaa
    11761 aaaagaccct acaataaagg caatgttagt aatgcttgat gcaattgaag aacgattact
    11821 aagcaaaaat gatgaggagt taaagatttt ttggcaaaaa ctgacatcag aaaacccacc
    11881 aattttattt catttcaagc aacttaatga tataggctta actgatgacc tttatataaa
    11941 aatgaatgcg agaggcaaag gattaactga ttttgagaac tttaaagcca aatttgaaaa
    12001 acatataaag aagaatgtct ttgaaaagga gcttgaatta agtgagaata ataaagaaaa
    12061 atggaaggaa cttacagaaa aaaccttttc acatagaatt gatacagttt ggacagactt
    12121 attttggaag catagaggtg aagataatat tattgataac gagatgataa aatttattgc
    12181 tggtatagca ataaataact atgctcaaaa tcttgaaatc ttggaaaatt cagaagatga
    12241 attaaggaca agaaaaattc tcgaagaaaa gaaagaaaaa aatattactg acgaagctgt
    12301 taaaagagaa agaatagaac gcagaattac agcccttttt aataatcctg ccgatgttag
    12361 tccttatgat tttcctacga aagaatcttt tgaatattta aaaaaatgct ttgatgttta
    12421 ctcaaaaaat cagaatgatg aattacttcc agccaatgta actttatggg atttttgtaa
    12481 aaccaaaaaa gtaaaaatca acagtgatac agaaattgat aataacttgt tcattgaatt
    12541 tattaaaaaa aatgaaacta cttattccca aagagtattg ttctatgcac aaacacaata
    12601 cttggtaaat ttagaaaact ttgattccga atcattttca aattggctaa gagtagtgag
    12661 gaacatcgtc caaaatgcta caattgatag tgcctatgct tttattggtg caattggttt
    12721 aatcaatgaa ctttcaagtg gatgcgaaaa tatttatgaa tttctttcca gtacaacgct
    12781 taaatccgga tttgcgacaa aacaaatcga ggaggaaaaa ttaaaagcgg cattgataaa
    12841 atctgataat aattggaaat cacaaatatt caaagcagaa gacgacattt tccttaaagg
    12901 tgaaattaaa tttctattag atttttctaa agttggcgag gaatataaga ttcaagaatt
    12961 taccagtatt tctgacaaat ttttattgtt atttgaaagt aaggatgatt tattacgaaa
    13021 agccttacta acacaagacg actatccagt ttgggacggt aggacatctt ctcttggttg
    13081 ggctcataga tactcattat tgaatactga aaatgaatgg aaagatgctt tcagaaaaaa
    13141 atcggataag tttataaatg cagtttataa gttaatttcc aattcgttta atttgagtga
    13201 tgacagaatc acaattttaa aaaatgtgat tcagaacgct acacctttaa atgattatcg
    13261 aagccattta attaaaaatg actctctttt gagagactgc tatcataaaa gaatatgttt
    13321 aaacgaaaaa aaaaccatac tatatgtact tgagaaaacg agggttgtag gtgaaaatta
    13381 caaagaaatt aaagtataaa acaactgcga catatattgt cacactctgc ctgtatcttt
    13441 gttcaaaata ttacagatat ggagcagaat ataaaattta gattattgga aagtataggc
    13501 aaagtatatg agcaatcaaa agattgcaag cttgattcag cattttttga gaaagttgat
    13561 gttgaattaa aacatctttc cgattatttt aaggtttcta gaaatcagtc attaattgtc
    13621 gcaatggttt tcgccctcaa ttacaaaggt gatactgttg atttgaaaga cctgattgag
    13681 tattttgact gtaatccaat gaaattattg gaattcagtg atgatttcga tgtgttatat
    13741 tcaaagggta tttttaaaaa gcaaaaatct tcacacagag ttagattagc tctttcaaac
    13801 gaccagttta caataaatga gaaaattaca gaagcaatct tgaacaataa accgatgcct
    13861 gtaattgagc gtgaaggatt taaagatgtg atagaacttc ttgaaaaatt tcataagtta
    13921 ggcgaacaac gagatagcga agaactatct acttttgagc tattcgccca agctaaagag
    13981 cttatttctt caaatttaca tttccctctt atcaagaaaa taaatgatta ccagtttagt
    14041 atcgaagata catatctatt actttacttg atttgggaaa cgataacagg gaatgaaact
    14101 actgatattg gacgtgcgac agagagtata tttgataatc cttcagaaag agttaaatat
    14161 gttcaaaaga ttatgtcgaa tgaaaataag ttaatcagtc aaaatcattt agaaatagtc
    14221 gaagctaatt tctttagtta taccgaaatg aagctatctg aatcttcggt taagatgcta
    14281 caagaattag ggttaaaact tttcgctaat ataaagaaac gtgataatat tattgaacca
    14341 gcaaaaataa actctaaaac tctttatttt aatgaatttg agcgtaaaca gctcgattta
    14401 ttaaagacat tgttacagga agataatttc aagaaaacgc aaaatagatt acaaagtaaa
    14461 ggtttgccga aaggtattac tgctttattg catggttcgc ctggcacagg aaaaacagaa
    14521 actgtttttc aagttgctaa ggacactaat agggaaattg tgaaagttga tattagtcaa
    14581 tcaaaatcaa tgtggtttgg tgaaagtgag aaaattataa aacgtatttt cactgattac
    14641 aaggcttatg caaaagactg tgagcgaatg cccattttat tgttcaacga agccgatgca
    14701 attattacga aacggaaaga gaatagcacc tcgaatgttt cacaaaccga aaatgcaatt
    14761 caaaacatta tacttgaaga acttgaaaat tttgaaggta tattttttgc cacaaccaac
    14821 cttgcaaaaa atcttgattc ggcatttgaa aggcgttttt tattcaaaat tgaatttcaa
    14881 aaacctgata tttcggtaaa agccaaaata tggaattcaa aattaccaag tctttctgaa
    14941 tcagaatgtg aaatacttgc aaaccgcttt gatttctctg gaggccaaat tgataatatt
    15001 gttagaaagg ttgagataca tgaaataatt cacggtatta cagtagattt taatagtatt
    15061 cttgatttct gcaaaacgga attattatca aaaaacagag gagttcgaat aggtttcaca
    15121 aaagcataaa aatcatgtcg aagagagaat caattgcacg atataacctg ataattaaaa
    15181 agctcagaaa acagccgtct tcattttcgg aaatttctga ctacctcgct tttgaatcag
    15241 atttgcagga atacaatttc aatgtatcaa aacgcacatt tcaaagggat atcgaagata
    15301 ttcgttcgat ttataatatt gatattcaat atgatttttc gaaaaaggtt tattatattg
    15361 actttgacga gcaaccagaa gtaaacgaga gaattttaga ggcttttgat acttttaacg
    15421 ctttaaatat tagcgacagg ctttcaaatt atattcactt tgaaaaacgg cggcctcaag
    15481 gcaccggaaa tctatatagt ttgctacatg ctataaaaaa taaggttcaa atatcatttt
    15541 cttatcaaaa attctgggag aatacgtcaa cccaacgagt tgtagaaccc tatgctctta
    15601 aggaatttaa aagtcgatgg tatttacttg ctagggattt aaaggataaa aaagttaaaa
    15661 gctttgctct agaccgttta acacaattag taatcacaaa aaaacaattc gtatatccta
    15721 ttgattttga tgtgaatgaa tattataggc attgctttgg aataattggg ccgaatgcga
    15781 aagagcctca agaagttatt ttgaccttta gtcgctttca ggggaattat attaaatctt
    15841 taccacttca tgaaactcaa caaatagagt ttgaaaatga aaatgaactg agaataaagt
    15901 taaaaatatt catcacccat gattttatta tggaattact gtcatttggg gagaatgtta
    15961 aggttatcaa accagaaagt ttagttaata gcattaaaga agtgtataat aaagcgttaa
    16021 acttgtataa atcaaatcaa aatgaaaaag ggaagagtga ttaatgttac ttcaacattg
    16081 gaagcagcag atttccatct gctttctgtt gatttccaac aagaaccaaa tcaatcacat
    16141 ttctgtaagg aaggaataca ttagatcttg catttagtgg gacttggagg aggaggttcc
    16201 ctgacattag agcattacta caatcagggg ttgcaagcta aattgacatg tatagcatca
    16261 aaagaacttc caaagtttcc ttcaaaaata aagtctattc actgcaacgt tcccaaaaaa
    16321 tatgacagca tagatgattg gtcgaattac tacatagctt tgcccgatga agtgaaatct
    16381 gtgtttgact caaatgagca ttttataatt ttagtcgggc ttggaggttt aacaggaaca
    16441 cttctaggaa aatctatttt caaacttctt aataacagaa atgcaaattc aatgtcgttt
    16501 agttaagcaa aaggacagga atagctcata cagaatagga tataggggaa aatagttctt
    16561 tgaaaagttt gtaatttagg atgagtacgc ttactttgta ggggtataaa gggggtatcc
    16621 ctagtattaa aagcaatttc gattaccgtg agtatttatc aaaacacata gtgtatgaac
    16681 ttgaaaatga aagttttaga ggccgttcta aaaatggcct caaaggggca ttcacacgcg
    16741 ataggaaact cacaatcaat aacttaatca tattggtaat caattttaaa agttccatac
    16801 aaagggagct tgactcattt ttcaaagcca tagcgaaaag cgactttaaa atacgggaag
    16861 taaccaaagg tgcattttcg caggcaaggg ctaaactaaa cccatgggca tttcagcgat
    16921 taaatgaggt tgctgtcaat gcgttttatg aaaaggcaaa ctatgatgta tggggtggtt
    16981 tacgggtagt ggctgttgat ggcacaaggt tggtacttcc aaaccatccg agcgtgattg
    17041 aagagttcgg gcaacaccag tttggcccca aagcagatag tccaaggtca ctggccatgg
    17101 ggtcgatgct atatgatgtg ttgaaccaaa taaccattga tgcccaattg gcaccttacg
    17161 catcgagcga aagggatttg ttgatgaaac accttggcca tgtagaagaa ggggacttac
    17221 tcttattgga ccgtggctat ccttgttttt ggttgctttt cctgctaaag gcaaggaaaa
    17281 ttgagttttg tgttaggtta aaagatgatt ggtggttaca ggtaaaagac tttacccgga
    17341 gtgacgaaaa agaacgtttg gtgacctttt cactacccaa aaaagatgtt aaaaagctat
    17401 cggagtaccc tgaaatgctt gggcaaacta taacttgccg attagtgaaa gttgagcttg
    17461 agaatggcga tatagaaata ttgtgcacct cgttactgga cgaccagtat tacacccata
    17521 aagacattga ggagctttac cattatcgct ggtcggaaga agaggcctac aagttgctaa
    17581 aaagccgcgt ggaagttgaa gccttctccg gcaaaacagc tacagcagtt aaacaggact
    17641 tttatgccaa agtattcctt atgactttat gtgcggcata tgctcatccc atagaagaac
    17701 gggttactgc cgaatataaa gcagataaag aaagaaagca cgatcaaaaa atcaaccgca
    17761 ctaatgcgat ttcaatgacc caggatattt taatcggagt ttttatccga aagcagttta
    17821 aagctgcgtt agaagctttt gatgatattg tacaaaacac gcgtgaaata atccgaccaa
    17881 atagaaaagt acctcgaaat aaaaaaccga agcgacttta ctatatgaat tacaaacgat
    17941 tatgaatatt ccttaactaa acgacattga aatataatta aaaacaaaga aagatgctcc
    18001 actttaaagc atctttcttt attaagtttc tttcaaatta actcattata aatcaacgtc
    18061 gttcagttag ggttatttgt catgttgaat cccgacatat cgggataggc cctgccgaat
    18121 catctcaaaa ctatatataa atagagatcc ttcgctgcgc tcaggatgac aaataaagtt
    18181 gtttgtgtat tgttactcgt tgttagtgaa actaatgcca tgttgagcgc aagcgaatca
    18241 tcactctgtc atgctaagct cgccgaagca tctcttattt ctttttcact tttagcattg
    18301 actggagatc cttcgctaca ctcaggatga caaaagaagt tactgttttg ttatctaaac
    18361 gacattgata tacctatagt aactaatgtt ggagttttta aggtttggcg gggtttttac
    18421 tttgttctgg ttaattggaa aatttgataa tttttccctt agaacaccct aacatccatc
    18481 gatctcgcgg aaatttaaac cgtgcggtta gaaaatattt ttccttaata taaatcccca
    18541 aaagaaggtt cgcggacttt tttccatgcc tttatctgaa acttacgctc acataccact
    18601 gtggaaccta gccaaacatc tcctttacct tatggagggc ttccaccagt cggtcaactt
    18661 cctcaaaggt gttatagaac gccagcgagg ctcgcacagt gccatcgatg ccgaagtggt
    18721 caataacggg ttgggtgcag tggttgcctg tacgaacggc tatgcccatc ttatcgagta
    18781 tcatgccggt atcgtacgga tgaattccgt tgagcaagaa cgagatgatg gctgcctttt
    18841 ccggtgcatt tccgtaaatg gttagcccat ctatctcttt tagctttgta gtagcgtatt
    18901 ttagcagctc atcctcgtat gcggtggcaa tatcggggcc aatgcttgaa tagtagttaa
    18961 gcgcggtagc caatccggct gcaccgatat agttggctgt tcccgcctca aacttgaacg
    19021 gcagctcgtt gaaggtggtt ttggcaaatg atacggttgc aatcatatcg ccaccaccct
    19081 gccagggcac catacgctca agccattcct ccttaccgta aagcacacct attccggttg
    19141 ggccataaat cttatgcccc gagaaggcat aaaagtcggc atcgagctgc tgaacatcaa
    19201 ttttaccatg ctttaccccc tgagcgccat caaccagcac ggggatgttt tggctgtgcg
    19261 ctatgcgcac aatctcggct ataggattaa ccgttcccag ggtgttcgac acctgcgtta
    19321 cagcaactat gcgggttttt ggggtaagta attcgggtag cttattgagc attagctcgc
    19381 cattgctatc aaagggaagc acctttaggg ttgcgccctt acgctcacag agcagctgcc
    19441 agggcacaat gttggagtgg tgctccattt cggttaccaa cacctcatcg ccctggcgga
    19501 caaacacctc gccaaacgag tatgccacaa ggttgattga tgcggtagtc ccagaggtaa
    19561 atattatctc ggatgttttt cgggcattaa ggaatttgcg aacaacctcg cgagcattct
    19621 catactcacg tgtcgattcc tcgctaagct tatgaacccc acggtgaata ttggcgttta
    19681 tctccctgta gaatcgctca atggtttgca gcacgcaaag gggtttctgg gttgttgccc
    19741 cattgtcaaa gtaaaccaaa ggccgcccgt aaacctcgcg ggataggatt gggaagtcag
    19801 cacgaaagct tagtaggttg ttcgttgttc gttgttcgtt gttcgtgtta aacatcactt
    19861 tacttttttt gatagtaatt aatgaagccg tttattaaca ttttgcagtt ttgtagcttt
    19921 tggaagagag cgtcataatc atgagtatca actttaaaaa tctccttgca caaaataagt
    19981 tgagtttcaa gttcaaaaat tgaacctctt gcaataaaaa ggaactggat tgtttctttt
    20041 gaagtgtttc gcccaatacc ctcggcaata tttgaaggaa ttgaaacagc tgcgcgtctc
    20101 atttgatggg atagaccata ttcttcattt gatggtaatt gcctagtgaa atggtaaact
    20161 aattttgcca attcaattga agacttccac acatcaagtt ccttatagtc cataatatta
    20221 aggtgttatt tcgaacaacc atcaactatc aacgaacaac gaacaactat caacgaacct
    20281 tcccctcttc aacccctttc agtccctccc taacagcaat gcattggaca gttgtggcag
    20341 cgcgatagtt caccgcgaag gcgtttgttg accatatcgt cgatgcgttc cctgagggtg
    20401 tcaatggaaa ttcgcttaac tacctcatgg gcaaaaccga acatcagcag cagttttgcc
    20461 tcgcgctcgc ctattccgcg tgcccgcatg tagaaaaggg cttcgttgtc gagctgacca
    20521 accgtggctc catgggagca tttaacatca tcggcgtata tctcaagctg tggcttggta
    20581 ttcatgcggg catcggctgt gagcagcagg ttgttgttcg actggtaggc ctgagttttt
    20641 tgggcatcgc gcctaaccat gatcttgccg ttaaaggcac cggtagcctg atcgtcaagt
    20701 attcccttgt agagctcatt gctggtgcaa ttgggaacag catggtcaat gaaagtaaag
    20761 ttatcggtat gctgggtgcg gtcagtaagg tgaagaccgt agagctgtgc atcggctccc
    20821 tcacctgcaa gtgtaacagc aagattattg cgaataaaac caccgtgaag cgaaagaacg
    20881 ttggtaacca gtctggaatc ggcctgctgg tgtgcatagg tgtgggtaag ctgagctgca
    20941 tcgttatgct cattctgcat cttcacaaac tcgagctggg cattgttgcc cacaaaaatc
    21001 tctgtaacca cgtttgatag gaaccgcttg ggtgagaggg tgtggtcgca aattaggaca
    21061 tttgcctgag cgccttcctc aaccacaata aggttgcgat actgcaccag ctgattcaca
    21121 tcggacatga gcaggttgat aatctgaatg ggcttgctct ccaccacatt ccgggggatg
    21181 taaataaaaa caccatcctg ggcaaatgct gtattcagag ctaccaaacc atcggtttta
    21241 ttctcagcca gtgagttata ccttccgttt accaaattgg gaaacttaat agcagcttcg
    21301 gccagactcc cgaaaataac accattgggc agagtggtta gcttttcgcc ctttggcagg
    21361 taaaatccgt taagcaccaa tgccaagtgg gtatcgagtt caggaatatc gcagcggaat
    21421 atatcatcaa cgttaaaagc tatgcgttta ggggcaaagt gcttttgata ctcatgtgcg
    21481 aatagcggtt caatgcggga atacttatac ttctcatttt tctgcgatgg aattccaagt
    21541 tccctaaagt gctgcatggc ctgttcccta tgcctattca ggatatcggc gctaccctcg
    21601 gtaattaact tgaggttatt ctggtaaagg ctaaccagct cctcgagtat gggtgttgct
    21661 attgttttat cggacatatc gtttacattt tatcaccagg gtgattaggc attttcacca
    21721 ttcttaatcc aatcgtaacc cttttcttcc agctcaaggg caagttcctt gccggcgctt
    21781 ttcacaatgc gaccattata aaggatatgc acataatcgg gaactatgta gtcgagcaag
    21841 cgctggtagt gagtaatgac aataactgca ttatcgggtc gtttgagctt gtttacacca
    21901 tttgccacca cgcgtagtgc atcgatatcg aggcctgagt cggtttcgtc gagtatggcg
    21961 agcttgggtt ccagcatggc catctgaaaa atttcatttt tcttcttttc gccaccggaa
    22021 aagccttcgt tcaccgagcg gttggtaaga gccgaatcta tttcgaccag ttccttacgc
    22081 tcacgcatca ttttcaggaa atcagaagcc gataggggct ctaatcccct gtacttgcgg
    22141 tgctcgttaa cggcagcttt catgaagtta accatgctca caccgggaat ctcaaccgga
    22201 tactggaagc ttaggaagat tccttcgcgt gcacgcacct cgggcgacat ctccaacaaa
    22261 tcctttccat tgaaggtaac ggagccgtga gtaacctcaa ccagctcacg accggttaaa
    22321 accgatgcca atgtgctttt tcccgagcca ttgggcccca taatagcatg aacctccccg
    22381 gcttttacct caaggttgat gcccttgaga atctctttat cgttgatttt ggcgtgtaaa
    22441 tccttgattg ttagcatatg ttttactttt gattatttac tacaattgtt gtttaaaatt
    22501 aaatgaattt gaggaagtta gaggaagtta aaggaagtta gaagaagttg ttgaagattt
    22561 atcatcgttt ttggcaattg ttgaactata agagtttaac agaaaactta cttcattaat
    22621 actttcatcc aatagttcaa atgtgcttaa atctatatat cccaaatcct tactcaaaat
    22681 agtgtaatat cgggtttctt ctaaactacc ttgagcgtaa tttagaaatt taagcttatc
    22741 caatctacct tttcttttat atccttcagc aatatttgct ggaattgaaa ttacagatcg
    22801 tctaaactgg gatgttaatg aataaatctc ttcgtgaggg aacttttggg taatggaata
    22861 aaccaaaagc gtaagtttat gggctttttg ccatactatc aggtcagtaa atacgctcga
    22921 tttataactt gaattcatga tctattagcc attgattcct ctaaccccat caactccctc
    22981 taacttcctc taacttcctc taacttcctt taacttcctc tcatataacc atcctaccca
    23041 acacttccct ccaaacttat ctgtagcagt ttttgtgctt caacggcaaa ctccatgggt
    23101 agcttgttga gaacttcctt ggcgtagcca ttaattatca gcccaacagc atcctcggtt
    23161 gacagtccgc gctggttgca gtagaatatt tgatcctcac ctatctttga ggttgtagcc
    23221 tcatgttcca ccacagccgt tggattatct acctcaaggt acggaaaagt atgcgctccg
    23281 cacttatcgc caagcagtag cgagtcgcac tgcgagaaat tgcgggcgtt ttctgcacct
    23341 ttgagtacct taaccagtcc gcggtagctg ttattgctat gccctgccga aatacccttt
    23401 gagactatgc ggctacgggt attcttgcca atatgaatca tcttggttcc cgtgtcggct
    23461 tgctgacggt tgttagtaac ggccacggag taaaattcgc cgtatgagtt atcgccaagc
    23521 agaatacagc tggggtactt ccaggttatg gccgagccgg tttcaacctg tgtccacgat
    23581 attttactgt ttctgccctt gcatatgccg cgtttggtaa caaagttgta aataccgccc
    23641 ttaccatcct tatcgccggg gtaccagttc tgtacggttg agtatttaac ctcggcattt
    23701 tccagtgcaa caatttcaac aaccgcagcg tgtagctggt tctcatcgcg catgggtgct
    23761 gtacatccct caaggtacga tacgtagcta tcatcgtcgg caacaatgag cgtacgctca
    23821 aactggcctg tattagctgc gtttatacga aaataggtgc taagctccat ggggcagcgc
    23881 acaccctttg gaatgtaaca aaacgaccca tcgctgaata cagccgagtt gagagccgca
    23941 aagtagttat cggtcaccgg aactaccgac gccaagtact tgcgtactaa atcgggatgc
    24001 tcacgaatgg cctcactcat tgagcagaaa atgattccct gctcagccag ggtctcacgg
    24061 aaagtagttt taaccgaggt gctatccatt actgcatcaa cagcaactcc ggcaagcacc
    24121 ttttgctcgt cgagtgggat cccaagctta tcaaatgttg ccttaatttc aggatcaaca
    24181 tcatccaggc tgtttagctt tgcattttgc ttaggcgcag cgtagtaaat aatatcctgg
    24241 tagttaattt caggtatctc aaggtgtgcc catttgggca tttccatggt ttgccaatgc
    24301 ctaaatgcct taagcctgaa ttcaagcatc caatcgggtt cccctttctt ttccgaaatc
    24361 attcggatta tatcctcact aagtccttta ggtatggttt cggtttcaac gttggttaca
    24421 aaaccgtatt tatactcgga accagtaacc tcttcaagta tcttatcctg ttcttctgcc
    24481 atatcggttt aacactttaa caaaatagca acaaactaca aaggctaatg tttagcgatt
    24541 cacagttaaa ttagatttaa tctaaattag gtgcaaagat atttgcttta agttgaatat
    24601 gaaaaggaaa tgaactaaaa taatggtatg ttttatatca acttgtatat ttcagaatgt
    24661 cagtaaaata aaaagcccca actggggctt taatcaaata actggaatac cattaaaagt
    24721 taaggttcac ggcaaccact tccttaattt caggaacatc gcgaataata gcttgttcca
    24781 caccattctt aagtgtcata atgctatatg ggcaactacc gcaggcacca tgaagtttaa
    24841 ctttaacaat gtaatcgtcc gttacctcaa caagtgagat atcgccacca tcgttctgga
    24901 ggaatggtct gattacttca attgcctttt ctactttggc tacaacttgt tctttggaca
    24961 tgacttgtat aattgatttg gttatttatg ttgaacatta actatctgag tgggtggtaa
    25021 ctccctgttg cgccactcaa cgcgctcaat caggtttttg gcaagctcat cgaaggcctt
    25081 accggtgatt gaggcatgaa ttgctacggg ttcaccgtaa tcgccaccct cgcggatgct
    25141 ttgaactatg ggaatttgac caagtagggg tattttcatt ctatcggcca gcttcttgca
    25201 accctccttg ccaaaaatgt aatatttatt gtttggtagc tcctcagggg tgaaccatgc
    25261 catgttctca actaaaccaa gcacgggtac attgatgctt gggctggtaa acatgcttat
    25321 tcctttcacc gcatcggcca aagcgacatc ctgcggggtg ctaactataa ccgcaccagt
    25381 taccggaact tcctgaacta aagtaaggtg aatatcgctg gttcccggag gcatatcaat
    25441 gaaaaggtaa tcgagctcac cccaggcccc ctgatggatt agctgcttaa gcgcactggt
    25501 agccattggc ccgcgccaaa caagagcgct attgggatca acaaaaaaac caatggaaag
    25561 taatttaaca ccgtaatttt caacaggaac aattagttcc attccctctt ccttaacaat
    25621 ctcgggctgg gtgtgctcca tgttgaacat cttggggatt gatgggccat aaatatcggc
    25681 atcaatcaaa ccaaccctgg ctcctgtttt tgcaactgca acagcaaggt ttgccgcaat
    25741 ggtcgattta cccactccac ccttaccaga ggcaatggct atgatattct tcaccttact
    25801 gattgatgcc ttttgcttca agggcgatac cttgggtgcc acatcggcaa ctaccaaaat
    25861 atctacctct acattctcgc caaacttctg ctggatggct ttagtagcag cttttttcaa
    25921 cgatgatgcg aatgggtcgt tactctttgt tagcttaagg gtaaacgata ccttactgcc
    25981 ttcaacgttt aacccctgca ccattcccat gcttacaatg tccttactgg tttcggggtg
    26041 caaaacagta gaaagcacct cattaaccga atccaatgat aaaaccatag tttttattta
    26101 gtttaaatta accgcaaagt tagcttttaa attgaaacat cgaaagttaa acagctaaag
    26161 ttgtaaaagg ttgatgaagg ggaaaaggag ttttaagatt tagtccgtta aacgtgaacc
    26221 gtgaaccgtg aaccgttaaa agttaaatgt gtgactttag gggggaatga gtcagaatag
    26281 aaagaatgtg aaggagttaa aggaagttag aggaagtttt attccgtgaa acgtgaaccg
    26341 ttaaacgtta aacgttaaat gtgtgacccg tcctgaaaaa tgtctacaca aacttatgtg
    26401 tatggacaaa aataatt
//
LOCUS       DAOOWJ010000027        26415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1841_length_26415_cov_7.769120, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000027 DAOOWJ010000000
VERSION     DAOOWJ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 26415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26415
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1841_length_26415_cov_7.769120"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLW22_04325"
     CDS             complement(<1..911)
                     /locus_tag="PLW22_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73965.1"
                     /translation="MKKIVAILLGVSLLWACDPMEDTYQKLDDAKQPYKDVITYTLVS
                     ADYANASNLALNDAQTYDDSTLAKAIKTQLAFNERFAGKDYIPDILTKNFPALSKGSS
                     ANIQYDVQYNTPANILDLYSNNIISEQEYKDSVWKSSTLFVKSFTPSTIGRMPQVLLK
                     KFEGASTNAYRLVEYNYSEVEPTIDNAEFKYFEEDFETHTYTPSPSYTPIGENGWTQK
                     DTVTGSVKGIYYCRLYSNNHYAQITSNNTGEKNDVFLITKEIDLTDANSPILTFDVNV
                     GYWNATCLSVLISSNYTGDLNQIGTAT"
     gene            complement(930..>1249)
                     /locus_tag="PLW22_04330"
     CDS             complement(930..>1249)
                     /locus_tag="PLW22_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU73966.1"
                     /translation="LPKLKIGADVNWYAKNYADFDPTTRTTPDSKQDAWRMPNYYTID
                     LDANYRFKIGNLNATLYGNVNNLLNTEYIADATDGANHDEFTSYVFFGFGRTWSTGLR
                     INF"
     assembly_gap    1250..1349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1350..3325)
                     /locus_tag="PLW22_04335"
     CDS             complement(<1350..3325)
                     /locus_tag="PLW22_04335"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU73967.1"
                     /translation="MKRTIWCTLLMLLFLAPMLTLGQGVIKGIIVDSNNGNPLIGAAV
                     VVEGTTIGTSTSVDGSFNFKVPIGKHVIKFSYVGYLDFEKNVEVTGDVDLGTIKLSPS
                     LVDIEEVLVTASFVRDRTTPVAVSTIEPKVIVEKLGNKEFPEILKITPSVYATRTGGG
                     FGDSRIYLRGFDSNNIGVLINGIPVNDMENGKVYWSNWAGLSDVTANQQVQRGLGASK
                     LALSSVGGTINIITKSTDAQKGGSYYYGFGNDGFRKQNFTVSTGLMENGWAVTVSGGY
                     TTGNGYVQATNFEGYSYFANISKKINEKHTITFNAFGAPQWHNQRNNKHTIQEFRDNP
                     NGIKWNSDFGYRNGQIYTTGYAYNFYHKPQMSLNHYWKLNDNSMLTTQIYASLATGGG
                     RRVYGQNSTWLSRQFPSGLPYDVTALTQEGYYDYDWVIQQNAAAMNGSTCIIANAVND
                     HQWYGALSTFTTNIESLKLTAGFDGRFYNGVHAYKIDDLLGGKYFLDNKNINRPASQP
                     LYKGDYVNYHYLGQVLWLGLFAQGEYIKDNYSGFLSMAIASNNYRRVDYFQYEPGNRA
                     SAWHDFLPWNVKAGFNYKVYGEHNVFVNGGYIKRAPIFSNSFLNNTNTFNDGVKYERI
                     ITAELGYNYASRIFNVKVNLFHTQWLDKGLVKSL"
     gene            complement(3456..4685)
                     /locus_tag="PLW22_04340"
     CDS             complement(3456..4685)
                     /locus_tag="PLW22_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOU73968.1"
                     /translation="MVFLYNLSIRFYQLLVFVASAFNTKARHFYRGRVGIWEKLKSQI
                     EPNSEIIWVHCSSLGEFEQGRPVIEAIEQKKPNAKILLTFFSPSGYEVRKNYSGADYI
                     FYLPLDTYSNASRFIKMVNPAMAIFIKYEFWYHYLNELRKKIIPTYLVSGIFRPDQVF
                     FKPYGAWYRKFLKAFTYFFVQNQQSKDLLASIGYTNVMVTGDTRFDRVSRIASDAKSI
                     PIVENFASNHKVLVAGSTWPKDEDIILPLVTKFPDLKLVVVPHEVTEAHIKDIMGKSS
                     VPAVRFTKSNPEEVKQAKILIVDTIGLLSSIYRYGHVAYIGGGFGVGIHNTLEAAVYG
                     IPVIFGPNFQKFQEAKDLIACKAGFSIKTYDELVDLLNLLLFDKNFYQLSASEAKQMV
                     NSGVGATQKILEVITAP"
     gene            complement(4774..5583)
                     /gene="lpxA"
                     /locus_tag="PLW22_04345"
     CDS             complement(4774..5583)
                     /gene="lpxA"
                     /locus_tag="PLW22_04345"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOU73969.1"
                     /translation="MTQHSNAFIHPDAKIGKDVTIGPFTYIAGDVVISDGTWIGPNVT
                     IMDGARIGKNCRIFPGAVISAIPQDLKFKGEITTAEIGDNTTIRECATVNRGTASRGK
                     TVVGSNCLLMAYSHIAHDCILKNNIIIGNGTQIAGEVEIDDHAILSAHVLVHQFERIG
                     AHVMVQGGSKVNKDIPPFIIAAREPLTFAGINLVGLRRRGFTAQQIGTIQELYRNIYN
                     KGMNVSQALAYIEENMEATPEREIVTSFIKDSKRGIVREYTGSNPDEGVEL"
     gene            complement(5583..6980)
                     /locus_tag="PLW22_04350"
     CDS             complement(5583..6980)
                     /locus_tag="PLW22_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOU73970.1"
                     /translation="MADKQRTIAQAVTLTGKGLHTGAEVEVSILPAEVNTGIIFQRTD
                     MDGQPKIEAVADNVIDTSRGTTIAVKNAKVSTIEHMMAALYSMGIDNALVQVNGPEMP
                     IMDGSSKYFVEAIAKVGTVEQGAERNYLCIKEKMVYTDEKNNIEIIIYPDDKLSIDVM
                     VDYNSRVLGHQYAKMNSIDEFPEQISPCRTFVFFHELEVLLKHNLIKGGDLENAIVVM
                     EHKVPQEELDRIADLFNKPHIAVKPEGILNNLDLHFDNEPARHKLLDMVGDFALVGAR
                     IKGKIIAMRPGHKANTEFAKILRKHYKQERLKPVAPDYDPNKTPLMDVNQIMKILPHR
                     PPFLLIDKILYMDGTTVVGMKNVTMNEAFFVGHFPDEPVMPGVLQVEAMAQTGGILVL
                     NTVPDPENYLTYFLKIDQVRFKRKVVPGDTILFKCFLNEPIRRGIANMTGQAFVGDQL
                     VMEGILMAQITKVKE"
     gene            complement(7039..8085)
                     /gene="lpxD"
                     /locus_tag="PLW22_04355"
     CDS             complement(7039..8085)
                     /gene="lpxD"
                     /locus_tag="PLW22_04355"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOU73971.1"
                     /translation="MEFTAKLIADFLGGSVDGDPNVKVHTVAKIEEGFPGSLAFLANP
                     KYIPYIYTTKASIVLVNNDFVPDQPVEATLVRVNNAYEAFASLLNLYAQSKQPKSGIE
                     EPSFIHKSAQIGEDPYIGAFSYISEGAVLGNNVKVYPQVYIGPKVKVGDNTILYPGVV
                     IYEECVIGSNCIIHSGAIIGADGFGFASSEETNYKKIPQIGNVIIEDYVEIGANATVD
                     RATMGSTILRKGVKIDDHVHIAHNVEVGENTVMAAQTGVAGSTKIGKNCMFGGQVGVS
                     PHVKIADGVKAGAQAGISGDIRKPGVIVLGSPANEIGKQRRSMAVYKNLPELMNKIHE
                     LEQVVEELRSKLNK"
     gene            complement(8128..9387)
                     /locus_tag="PLW22_04360"
     CDS             complement(8128..9387)
                     /locus_tag="PLW22_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU73972.1"
                     /translation="MNGTNKKKIVNDPVHGFISISHPLIFDLIEHPYFQRLRNIKQLG
                     LSYLVYPGAVHSRFQHCIGAMHLLDLAIETLRSKGLDISDNEAEAAAVAILLHDIGHG
                     PFSHALEHVIANDISHEHLSRCFLYQLNKEFDGKLSLAIEIFEDKYPRHFLHQLISGQ
                     LDMDRMDYLKRDSFYTGVSEGIIGTERIIKMLNVVDDTLVVEAKGIYSIEKFLIARRL
                     MYWQVYLHKTVVAAEQVLIKLLERAKELVLQGHDIYCNEPLLFLLNSKLTKADFFPNP
                     QMPEAPLPLQHFAYIDDNDVMVAAKQWIHHGDKTLSQLANMIVNRRLPSVELNSTGFD
                     SQRLSRLMAEFRKQYIDLADFSDYFVFTDTISINAYKPGEEAIQILYNNGKLVDIAIA
                     SDMLNAKALSDETKKFFLCYPKQLRGI"
     gene            complement(9441..9827)
                     /locus_tag="PLW22_04365"
     CDS             complement(9441..9827)
                     /locus_tag="PLW22_04365"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU73973.1"
                     /translation="MQKYELRETATTPYVNFDPDTGVIRMEGRSIPENVIDFYQPILR
                     WIDEYATNPQPETIVHLKFEYFNTSSSKRIFDIMKKFEKLAVDTGKKVTINWYFEEDD
                     EDIYFAGNDYKALINKVDFNLVEIKG"
     gene            complement(9877..10386)
                     /locus_tag="PLW22_04370"
     CDS             complement(9877..10386)
                     /locus_tag="PLW22_04370"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU73974.1"
                     /translation="MGILIEKTQDCPYVNFSDEGILEIEGRSITEDPFTFWQPLLEWV
                     EGYCQKPSPNTQVIIFLEYSNSSSNKYISEIFRKLEEIHGVKTQVSVKWRHEIEDDAI
                     LQLGHDFASIFELPFEFIGVDVEKERFKKVKIRSKKTGTEAIISYRYWDAIVRNGHGD
                     EYQILQEFS"
     gene            complement(10488..11927)
                     /locus_tag="PLW22_04375"
     CDS             complement(10488..11927)
                     /locus_tag="PLW22_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU73975.1"
                     /translation="MRKLFATNLIFLLLLNVVVKPLWIFAIDRNVQIVLGAQEYGFYF
                     ALFNFSLLFNFLLDLGITNFNNREISYNPQLISQQFANVLVIKLVLALVYFMVTLGIG
                     VSLGFGKRHIVLLAVLTLNQFLASLLLFIRSNLNGLHLFKADSVLSVMDKAIVIVLVS
                     LLLWGNLNIQLTVEVFALSQTLAYVVAIFIAIWLLQGKYSIAIYREFDRITIVTLIKK
                     GLPFAALVLLMTLYSRLDTVMVERLCPEGAVSAGIYAQAFRLFDAFNMYSYLFAALML
                     PIFSRMMSNQEDITELFNFSFNLILIPVVAIAVPTMIYNRPVMSVLYHEHIDVSSKAL
                     VLLMGSFVFVALSYLFGTALTAKGKIWLLCRISLGAVIVTLFSMLILIPRFGVIGAAM
                     ANLVANAIASLLQAFFFIRSQGSAISKTSVLKISLYILIYSAFGLMIQYSVGNNLTGF
                     IATLASSLLVVFGLRLFSFRAIINFARGI"
     gene            12073..12145
                     /locus_tag="PLW22_04380"
     tRNA            12073..12145
                     /locus_tag="PLW22_04380"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12106..12108,aa:Met,seq:cat)
     assembly_gap    12361..12460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12869..13993
                     /locus_tag="PLW22_04385"
     CDS             12869..13993
                     /locus_tag="PLW22_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73976.1"
                     /translation="MKKILFALILLASQTVLAQETVTIKFIRPSKVQGSAAKIRIIIN
                     DNEYVLKNGTETTVIIPHDYSKPLLVEASAGGTRQSYRLRAIPSEKYVFETGFEFKGI
                     YLKLLEGKELAVGEVVHKVDTAKAGGETTLKVDKDGRAVGFTIERIDESEAIRQEWLS
                     RGGRIKNESVLLTGTYFGMNLKDFDTRVDGYGGGISAYQNWINLKIPEFKSGISTWNS
                     FNYGWGIDMLLYRYKLNMDLSPSGSMDMSVLTLSYPISANLGWTFGIGKFLDQGNWKG
                     VALTLKYKPSLTLNMVSTTSTIKMPAPLNTTQTDYSSDTNASINLGGFGVDFEFSNFT
                     ATMNRLAPTPKTKFSFFVLPPVGDTPLFVSISLGISWYSR"
     gene            14120..16642
                     /locus_tag="PLW22_04390"
     CDS             14120..16642
                     /locus_tag="PLW22_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU73977.1"
                     /translation="MLQKIQNRNYWIKPKVIGGFLLIIIFSVTAIVMTYQAVSDLKTT
                     RQGLGLPGQKITVLNRLLASIYTEDSDFRMYVLTSNDDYLKTYNNNHQKVTAAIKSLQ
                     KLAYSNQSQLNSIKKIEKLLQNKREIEGEILQMRQSINTSIFYDRAMQRIARAGSETQ
                     RYHEVYKQKTVTQVRRDTVIYKKVDNESMAARIKRFFLGPERIDTIATDTLVEFTYDT
                     IPHITYISDSIIQRLLLILDNIRNDQQNQLRLIDEKEKELLNRNRAILNQVQLIVTSI
                     EKQEQAEAVSQALSVQEVLTKNLLIIFLLGTITFIVLVILIAIIMRDISRNSTYNAQL
                     IEAKQYAENLLRLKEQFLANMSHEIRSPLSAIVGVSRQLSKTELNGKQKEYVDVLNSS
                     SNHLMAVINDILDYSKLAAGKLRLESREFSPREVVNDTVKLFEARANEKGLQILSNID
                     QSLPHIVVGDDFRLRQVLINLLSNAIKFTEQGSIIVSGAVLRKTDDSLKIQFTVADTG
                     IGISPEMQSQVFEEFTQADGGISRKFGGTGLGLTIVKKLVELQGGEISLTSEPNEGTI
                     IRVVIPYSIKETRTANVDQETHYRIKAGTSILIIDDDEVNRLIVTEIAKNVGLEVDAI
                     SDAKNLIDQLKAKKYDAVITDIQMPEVSGYEVVQLIENAGYSIPVFAITANDMIDNPE
                     HFSSLGFSGYLIKPFTEADLIRLLGPVIGATGVVTGDKRERADTYNKFDIEEIYRFTG
                     GNKESVKLILESLLENTRKNVEELNQLIKSKNLTKASAVAHKMKSAFNQFRVYDIASI
                     LQRIETLPPDKHKAATLYMDRLNRRVKSFTVELQEQIDNLLN"
     gene            complement(16639..19953)
                     /locus_tag="PLW22_04395"
     CDS             complement(16639..19953)
                     /locus_tag="PLW22_04395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU73978.1"
                     /translation="MKKLLFLGILFISVLQASGQQFNKYGNPLITNYTPEVYGGNEQV
                     WCIAQDDRGVMYFGTNDNGILEYDGKTWRSIPMPENKSVRSLCKGADGVIYVGSTGEF
                     GYLQPTANGRLQYFSLIKNLPDSIQSNLSYIYKTYWYEGKTYFCSLSNIFIFDGNTIK
                     PISLGKQSEYANFFTLKANDKFYVNSYLQGLRTFTSDSTLEVIPGGQAFIHKNVFSIV
                     AIDSSKIFIFTDNGFYTYSNGKVESVDTPTLLAKRMATESAIPYSATKLNDGNIALGV
                     VQSDFFGVVTLAPDLKTINLVNKQTGMHAGQVSEVFQNGDAPLWATLYDGGIAKVELN
                     SAIGRFGQECGLSEIIVDIVRFNNTIYLATFNGVYYLAYDSNGVPKFIPVDGINGNVW
                     ALTIFSPPNAPKMLLAGSYTDGVFEIKGSKAISISNPLNANLELSKQIQHSCFALYPS
                     KSIPGRLYIGLNSGVAYIDWANGEWKHTDNLFRDSINFEIRSIVEDSKGNLWLATGYS
                     GVYMVNKSFTKLSKFGKESIKGLENLQYIILFPVNDSLYILTSNGIYHFNYENNLFEP
                     GGLVGSAFSKRFGIYKATKFSNGYAFLCYDSNNKNWIERVVKDSAGNWVTDNRDLKRL
                     PNKWADAIYADPDGTLWIGMSKELYVYNPHVKRSFNAPFRAVIREVVSKDSLLFGGAF
                     YTQGDSSVRKLSLEQQPYQMPRLGYHYNAMVFTFAAPYFEREEDIVYSHYLEGSDETT
                     WNQWDKRTDATYTNLREGKYTFHVKAKNIYGDESIEATYSFEIRPPWYRTILAFILYF
                     ILAVCLVWGIVKWNTRRLIAEKERLEQIVRERTAEVVAQKEEIEQQKEKIAAQNEEIT
                     SSIQYASRIQTALLTPTDQINRIFPENFILYLPRDIVSGDFYYITQAGKLKVSVVADC
                     TGHGVPGGFMSMLGISFLTQIIGTKTDLKANEVLNELRNLVITALHQTGEIGGSKDGM
                     DIAIYIIDEETQTLQFAGANNPLILIRNNELTHIKGDKMPIGIHLRGELSFTNNVIQI
                     QKGDVIYTFSDGYVDQFGGEDGRKFMIKHFKELLLEIHHKPMSEQRQILDETLKNWHG
                     NTPRIDDVVVMGVRIV"
     gene            complement(20380..22782)
                     /locus_tag="PLW22_04400"
     CDS             complement(20380..22782)
                     /locus_tag="PLW22_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73979.1"
                     /translation="MNKLKVLLLFLVVAITTAAQQPVGFSYQAVLRNSSGQVLANQNV
                     TLRLSLTDASGTTTYYSESHALQTTAQGVVSLTVGGGTVVSGTYSAVPWQGQEVRLRV
                     EVSTDGVNYSDMGSKAIQPVPYTIFSDSARVSYRSAESTISLFADSAARAGYADSINL
                     RFSRNLVVVGNDPANPDDPIFEVKNSKGEVLFGVYQEGVRVNIPDSAITKGAKGGFAV
                     GGLTGAKGEPVEYLRITPDSARVYVKQSTGIKGAKGGFAVGGLTNQTKAVIAQDLLFV
                     NPDSARFYINENLSKGAKGGFAVGGLTNGKTSAQLIQLTKDNYLIGYEAGASITTGQF
                     NSFLGFQAGKANSTGNWNTFLGYQTGLNNTGSDNTFIGYQAGMLHQFGGGNVYLGSKA
                     GGNALNGVQNIAIGEQSGFSTSTGFKNIFIGYMAGYSNTSGYSNIFIGNESGKSNTDG
                     NRNMFFGNGSGFSNTSGFGNTFFGLESGYYNVNGFRNLFLGYRSGYKNVDGSDNSFLG
                     DMAGSSNTSGNENTFVGQASGASNDTGRDNTFLGSNVGRGNTNGSGNVMVGRFTGYNI
                     NGNNNTFIGTQSAANFNTGPFIASSNVCIGYASGTGLTNGFYNIFIGQQTGTNNGFAN
                     VIIGHQAGLNCSGNSNVFIGYQAGISEAKSNRLHISNSGGDSTQALIYGRFDSKEIAL
                     NARVGIGTTVPDKQLHVVGDARITGNIYCNDVIKPDFVFKPDYSKRLDPLSVERFIAR
                     NGHLPWLTKASEERDGINLTRMQFETVETVENLQLQIIQQQKEIENLKAELETLKKLI
                     KQ"
     gene            complement(22910..>26415)
                     /locus_tag="PLW22_04405"
     CDS             complement(22910..>26415)
                     /locus_tag="PLW22_04405"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOU73980.1"
                     /translation="YNTTSKISYIWDGDTWEILAQDGLPGAQGPQGPEGPQGPAGTGL
                     NNRGDWVSGTTYDPGDYVFALNTGGTANSMWILEGTSPYLSTLQPRLDPDHWVEFEAP
                     AGPAGQNGISIQWLGTLPAPPSSPVTNQAYYNSVAKKAYIWDGDSWEILAQDGEQGPP
                     GPLVAGTTGQTLRHDGTSWAASSLIYNNATNIGINTTTPTQRLDVNGNMRLRGLLVDY
                     GNSSGTSGQYLSRGTSGVVWQTPSWVTGSGVSGQMAVWNGASSITNLPNLTFAQGLVV
                     VGNPTANPDDPIFEVKNSNGEVIFGVYQEGVRINIGDEAIKGAKGGFAVGGLTNQTKA
                     GPVEYLRITPDSARIYIKQDSGVKGAKGGFAVGGLTNQTKTVVAQDLLFINPDSARIY
                     LNENPTKGAKGGFAVGGLTNQTKASSPQLIQLTKDNYLIGYEAGMSITTGLYNSFLGY
                     QTGKLNNAGNWNTFLGYQAGLNNTGSDNTFIGYQAGMAHTTKGGNVYIGSKAGSNATN
                     GERNVFIGESAGYSIVSGFKNVFIGFESGYSTSTGNYNVFLGTTTGRANTSGSSNVFV
                     GIGSGFNNTTGSNNVFLGTSSGLSNISGNFNTFLGYDAGYSNTASYNLFVGYQAGRAN
                     TSGTYNTFLGFQSGYANVDGSNNIFIGLMAGMSNTSAGNNVFIGNESGRYNTTGTNNS
                     FLGNYTGRANTTGNSNVFIGNQSGRSNTTGNSNIAIGDYAGYSNQTGVSNIFLGKAAG
                     YTGTNLQYNVFIGDSTGYSTSSTYATTNVFIGYAAGRFNNSYNNVYIGNKAGYSCITG
                     QNNIAIGDLAGFKNTSSQNIFIGTSAGENNTEGYFNIMIGRWTGKNNTTGHQQILIGG
                     GAGSSLTTGNENVIIGTNAGSINSTGSGNIYLGTSAGWSNTGSKNVFIGYKIGYGTFQ
                     NVSNALVIGTDVNPTTPLIYGEFDNKKLKFNANVGVNVDPTGSKLFSYDDRTIAVDDP
                     AVLGEHKLNTTGWGIGVKGDGGYMGVYGVASVAGTGTRYGVYGYGANGTTNYAGYFSG
                     NVYVSGTFTNPSDERLKKNITPLKGSLAKLSKITGVTFEWKSNEEIKGIVSDKESNKG
                     IEKFNFPVGTQIGVIAQDVEKVFPELVQTDENGVKSVDYVKLVPVLIESIKEQQQQIN
                     NLQEQINELQRLLIEVVKQNK"
BASE COUNT         7774 a         5724 c         5063 g         7654 t
ORIGIN      
        1 catgttgcag taccaatttg attgaggtct ccagtgtaat tagaagaaat taaaacactt
       61 aggcatgttg cattccagta acctacattt acatcaaagg taagaatagg gctattagcg
      121 tcggttaaat caatttcttt ggtaatcaaa aatacatcat ttttctcacc ggtattattt
      181 gaggtaattt gggcataatg attattacta tataatctgc agtagtatat ccccttaacg
      241 cttccagtaa cagtatcctt ttgagtccat ccattttcac ctattggtgt gtatgatggt
      301 gatggtgtat aagtatgagt ctcgaaatct tcttcaaaat acttaaattc agcattatca
      361 atagtaggtt cgacttcgga gtaattatac tcaacaagcc tgtatgcatt ggtcgaggct
      421 ccctcaaatt tttttagtaa aacttgaggc attcttccaa ttgtagatgg ggtgaaagat
      481 ttaacaaata aggtggaact tttccaaaca gaatccttat actcctgttc gctaataata
      541 ttgtttgaat acaaatcaag tatgtttgca ggagtattgt attgaacatc atattgaatg
      601 tttgctgaag accccttgct tagagcaggg aaattttttg tcaatatgtc aggtatataa
      661 tccttacctg caaagcgctc attaaatgct agttgtgttt taattgcttt agctaaagtg
      721 ctatcgtcat aggtttgtgc atcatttaat gccaaattac ttgcattagc ataatctgca
      781 ctaacaagtg tgtaggtaat tacatctttg tatggttgct tagcatcgtc tagtttctga
      841 taggtatcct ccataggatc acaagcccac agcaacgaga caccaagtaa aattgctact
      901 atctttttca ttataaatcc tcccaatttt taaaagttaa ttcttaaacc agttgaccag
      961 gttctcccaa aaccaaagaa tacatatgaa gtaaattcat cgtgattagc gccatctgta
     1021 gcatcggcaa tatactcagt attaagcaaa ttattaacat tgccataaag tgtagcattc
     1081 agatttccaa ttttgaagcg atagttagca tcaaggtcta tggtgtaata atttggcatg
     1141 cgccaagcat cttgctttga atcgggagtt gttcttgtag tagggtcaaa atcggcgtag
     1201 ttcttagcat accaatttac atcagctcca atctttaact ttggaagaan nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc taagactttt tactaaccct ttatcgagcc
     1381 actgggtatg gaatagattt acttttacat tgaaaattct tgaagcatag ttatacccca
     1441 actcggcagt aataatacgt tcatacttta caccatcgtt aaatgtattg gtattattca
     1501 agaaggaatt gctaaaaata ggtgctctct tgatataacc accgtttaca aatacattgt
     1561 gctccccata taccttgtaa ttgaaaccag ctttaacatt ccaaggcaag aagtcatgcc
     1621 atgccgatgc gcgattacca ggttcatact ggaaataatc tactcgccta tagttgttac
     1681 ttgcaatggc catcgatagg aaaccagagt agttatcttt gatatactca ccttgagcaa
     1741 atagccctaa ccaaagtacc tgtcctaggt aatggtagtt tacataatct cctttgtaaa
     1801 gcggttgact tgcaggacga ttaatattct tattatccag gaaatatttg cccccaagca
     1861 aatcatcaat cttataggca tgtacaccat tataaaaacg tccatcgaaa ccagcagtta
     1921 acttaaggct ttcaatgtta gtagtaaagg ttgataaagc tccataccac tgatggtcat
     1981 ttacagcgtt agctataata catgttgaac cattcattgc tgcagcattt tgctgtatta
     2041 cccaatcata gtcataatat ccttcttggg ttagagcggt tacatcatat ggcaatccac
     2101 tggggaactg acgacttagc catgttgaat tttgcccata aaccctacga ccaccaccag
     2161 tagctaacga tgcataaatt tgtgttgtaa gcattgagtt atcgtttagc ttccagtagt
     2221 gatttaagga catttgaggt ttatggtaaa aattgtaagc atatcctgta gtatagattt
     2281 gcccgttgcg atatccaaag tcagaattcc acttaattcc atttggattg tcacggaact
     2341 cttggatagt atgcttatta tttcgttggt tgtgccactg aggggctcca aatgcattaa
     2401 aggttattgt atgtttctca ttaatttttt tcgaaatgtt tgcaaaatat gagtaaccct
     2461 caaaattagt agcttgtaca tatccatttc cagtagtata accacccgat acggtaacag
     2521 cccaaccatt ttccattaaa ccagttgaaa cggtgaaatt ctgttttctg aagccatcgt
     2581 taccaaagcc atagtaatat gagcctcctt tttgagcatc agttgacttt gtaataatat
     2641 taatggtacc cccaactgat gacaaagcca atttggaagc acctaaacca cgttgaacct
     2701 gctgatttgc ggttacatcg gatagtcctg cccagttgct ccagtaaacc ttaccatttt
     2761 ccatgtcatt tactggaatc ccatttatta aaactccgat gttatttgag tcaaatccac
     2821 gaaggtaaat tcttgaatct ccaaaaccac ctcctgttct tgtagcatat actgatggtg
     2881 ttattttgag tatctcagga aattccttgt ttcctaactt ttcaacaatt acttttggtt
     2941 caattgttga aacagcaacg ggggttgtcc tatcccgaac aaacgatgct gtaacaagaa
     3001 cttcctcaat atcaaccaaa ctagggctta acttaatggt tccaaggtca acgtcgccag
     3061 ttacctcaac atttttttca aagtctaggt agcccacata agaaaactta atcacatgct
     3121 ttccaattgg aactttaaag ttaaatgagc cgtcaacgct tgttgaagtt ccaatagttg
     3181 tgccctcaac taccacagca gcaccaatta aagggtttcc attgtttgag tcaacaatta
     3241 tcccttttat tactccctgc cctaaagtca acattggagc aaggaataaa agcattaaca
     3301 atgtacacca aatcgttctt ttcataggtt tttgatttta gagttaatta aggctaatat
     3361 tcatttatgc aaagttaatt tcatataata ccgaaaaatc acaaagaaac attaacaaat
     3421 tgttaaaccc ccgcaaacgt ttgaaacaaa ttgtttcatg gagcagtaat aacctcaaga
     3481 attttttgag tggccccaac gcctgaattt accatttgtt ttgcttcact tgccgatagc
     3541 tggtagaaat ttttgtcgaa gagcagtaag tttagtaaat ccactagctc atcgtaagtt
     3601 ttgatagaaa atcctgcttt acatgcaatc aaatcttttg cctcttggaa tttttgaaag
     3661 ttgggcccga atattacggg aataccgtaa acagcagcct caagggtatt gtggataccc
     3721 actccaaacc ctcctcctat gtaggcaaca tgaccatacc tataaattga cgatagcaag
     3781 ccgattgtat caacaatgag tatctttgct tgcttaacct cttcggggtt ggatttggta
     3841 aatcgaacag ctggaactga cgacttaccc attatatcct ttatatgcgc ctccgttacc
     3901 tcatgtggaa caacaactaa cttcaaatcg gggaactttg ttactaaagg taaaataata
     3961 tcctcatctt tgggccatgt gctgccggct acaagcactt tatggtttga tgcaaagttt
     4021 tcaacaatag ggatactctt tgcatcagag gctattctgg aaaccctatc gaatcgggta
     4081 tcgccagtaa ccataacatt ggtataccct atgctggcta gcagatcctt tgattgttgg
     4141 ttttgtacaa aaaagtatgt gaatgctttg agaaacttgc gataccatgc gccatagggt
     4201 ttaaagaaca cctggtcggg tcggaaaatc cctgacacaa ggtaggttgg gataatcttt
     4261 ttcctaagct cattgaggta gtgataccaa aactcatact taatgaaaat ggccattgcc
     4321 ggatttacca tttttatgaa cctgcttgca ttggagtagg tatcgagtgg taagtagaag
     4381 atataatcgg ctccggaata gtttttccga acctcgtacc ccgatgggga aaagaaagta
     4441 agcaatattt tagcattagg tttcttctgc tctatggcct caataacagg tcttccctgc
     4501 tcaaactcgc ctaacgacga acagtgtacc caaataattt cggaattagg ctctatttga
     4561 cttttaagtt tttcccatat cccaactctg cccctataaa aatgccttgc cttggtgttg
     4621 aatgccgatg cgacaaagac cagcaattga taaaatcgaa ttgacaggtt gtaaagaaat
     4681 accatataag attggttcta taagttcagt caaatatagc taaaggttaa acccgtacca
     4741 aaaaaaatgc cggcaaaagc cggcatgata ttcctaaagt tctacaccct catcgggatt
     4801 ggaaccagta tattccctca caattccccg ttttgagtcc ttaataaagg atgttactat
     4861 ttcgcgctca ggagtggctt ccatattctc ctcaatgtaa gctaatgcct ggcttacgtt
     4921 cataccttta ttatagatat ttcggtatag ttcctgtatt gttccaattt gctgagcggt
     4981 aaaccctcta cggcgaagac ctaccagatt aataccagca aaagttaaag gttcacgtgc
     5041 tgcaataata aaaggaggaa tgtccttatt aacctttgat ccaccctgaa ccataacatg
     5101 ggcgccaata cgctcaaact gatgcactag cacgtgtgcg cttaaaatgg catggtcgtc
     5161 aatttcaacc tcaccggcaa tctgggtgcc attgccaatt atgatattat tctttaggat
     5221 acaatcatgt gcaatatggg agtatgccat taggagacag ttactaccca ccactgtttt
     5281 cccccttgat gctgtacccc ggtttacagt tgcgcattca cggatggtgg tgttatcgcc
     5341 aatttcggca gtggtaatct cacctttgaa ttttaaatcc tgtggtattg cagagattac
     5401 tgctcccgga aatattcggc agttcttgcc aatgcgtgca ccatccatga tggtaacatt
     5461 tggtccaatc catgtaccat cgctaattac aacgtcgcct gcaatgtatg tgaatggtcc
     5521 aatggttaca tccttgccaa ttttggcatc gggatgaata aatgcgttgc tatgctgtgt
     5581 cattattctt taactttagt tatttgagcc ataagaattc cttccataac caattgatcg
     5641 cccacaaaag cctgtccggt catgttggca attcccctgc gaatgggttc gttgaggaag
     5701 catttaaaaa gaattgtatc gcccggaaca acttttcgtt tgaaacgaac ctggtcaatc
     5761 tttaagaagt aagttaaata gttctcagga tcgggaacag tattaagaac aagaatgcca
     5821 ccagtttgtg ccattgcttc aacctgaagt acacctggca ttaccggttc atcggggaaa
     5881 tgtcccacaa aaaaggcctc gttcatggta acattcttca tgcccaccac ggtggtacca
     5941 tccatataaa gaatcttgtc aattaatagg aatggaggac gatgcggaag aattttcatg
     6001 atctgattca catccatcaa gggagtcttg ttgggatcgt aatcgggggc aacaggtttt
     6061 aggcgctcct gcttgtagtg cttccgaaga atcttagcaa attcggtgtt tgctttatga
     6121 cctggacgca tggcaataat ttttccctta atgcgagcac caacaagggc aaagtcgcca
     6181 accatatcga gcagcttatg gcgtgccggt tcattatcga aatgtaggtc aaggttatta
     6241 aggatacctt cgggtttaac ggcaatgtgt ggcttgttaa atagatcggc aatccggtca
     6301 agctcctcct gtggaacctt gtgctccata acaacaatgg cattctccag gtcaccccct
     6361 ttaataagat tgtgttttag caacacctct aactcatgga agaatacaaa ggtacggcag
     6421 ggtgatattt gttccgggaa ctcatcaatt gagttcatct tggcatactg gtgtccaagc
     6481 actcttgagt tgtaatcgac catcacatcg atgctcagct tatcgtcggg gtaaatgatt
     6541 atttcgatgt tgtttttttc atcggtgtaa accattttct ccttaatgca aaggtagttc
     6601 cgctcagcac cctgttcaac tgttcctacc ttggcaatag cctccacaaa atacttggag
     6661 ctaccatcca ttatcggcat ttcaggtccg ttaacctgca ccaatgcgtt atcaatcccc
     6721 atgctgtaaa gggcagccat catgtgttca atggtgctaa ccttggcatt tttaaccgca
     6781 atggtggttc cccttgaggt gtcaataaca ttatcggcaa ccgcttcaat tttaggctga
     6841 ccatccatat cggttcgctg gaatatgata ccggtgttaa cctcggccgg tagtattgat
     6901 acttctacct cggcgccagt atgtaacccc ttacctgtca aggtaaccgc ctgtgctata
     6961 gttctttgtt tatcggccat tttgattaaa atttcagtta aacgacaaaa aggcgcattt
     7021 gagcgccttt gcatactgct atttattaag ttttgatcgc aactcttcca ctacctgctc
     7081 cagctcatga attttattca tcagctcagg caaattctta tacacagcca ttgatcggcg
     7141 ttgtttgcca atttcattgg ctgggcttcc taaaactatt accccgggtt ttcggatatc
     7201 gcccgagatt ccagcctgtg cgccagcttt aaccccatcg gcaattttaa catgggggct
     7261 cactccaacc tgtccgccaa acatacaatt tttgccaatc ttggttgatc ctgcaacacc
     7321 agtttgggca gccataaccg tattttcgcc aacctcaaca ttgtgtgcaa tgtgaacatg
     7381 atcgtcaatc ttaacccctt ttcgaagtat tgtcgacccc atggttgccc tatcaaccgt
     7441 ggcattagcg cctatctcca cgtaatcttc aattatcaca ttcccaatct gtggaatttt
     7501 cttgtagttg gtttcctcgc tcgatgcaaa tccgaaacca tcggcaccaa taatagcacc
     7561 actgtggatg atacagttgc taccaataac acactcctcg tagatcacca cacccgggta
     7621 tagaatagta ttatcgccaa cttttacttt tgggccaatg taaacctgag ggtatacctt
     7681 cacattgtta cccaaaacag ccccttccga aatatagctg aaagctccta tgtaggggtc
     7741 ttccccaatt tgggccgact tgtgaatgaa tgatggttcc tctattccgc tcttgggttg
     7801 ctttgactga gcatacaagt tcagaagcga agcaaaagcc tcgtaggcat tgtttaccct
     7861 gacaagcgtt gcctcaaccg gttgatcagg gacaaaatcg ttatttacca gaactattga
     7921 tgccttagta gtgtatatgt aaggaatata ttttgggttg gccaggaatg caagcgaccc
     7981 aggaaatccc tcctcaattt ttgccactgt atgcaccttc acgttgggat cgccatcaac
     8041 agagcctccc aaaaagtcag ctataagctt tgccgtaaat tccatattaa gtaattgtaa
     8101 acaatttgca aaactaatta atttaatcta aatgccccta agctgtttag gataacaaag
     8161 aaaaaatttt ttggtttcgt cggaaagagc tttagcgttg agcatgtccg aggctatggc
     8221 aatatcaact agttttccgt tgttgtaaag tatttgaatt gcttcctcgc caggtttata
     8281 ggcgttaatg ctaatggtgt cagtaaaaac gaaataatca gagaaatcag ccagatcaat
     8341 atactgcttt ctaaattctg ccatcaaccg gcttagtctt tggctatcaa agcctgtgct
     8401 attcaactca acactaggca aacggcgatt aacaatcatg ttggcaagct gtgaaagggt
     8461 cttatcgcca tggtgtatcc actgctttgc agcaaccata acatcattgt catcaatgta
     8521 ggcaaagtgc tgaagtggaa gcggagcctc cggcatttgg gggtttggga aaaaatcggc
     8581 tttggttagc ttgctattga gcaagaatag taaaggctcg ttacagtaaa tatcgtgacc
     8641 ctgaagcact agttctttag ccctttccag caacttgatt agcacctgct cagcagcaac
     8701 aaccgtttta tgaaggtata cctgccagta cataagccta cgggcaatca ggaatttttc
     8761 aattgaatat ataccctttg cttccaccac caaggtgtcg tcaaccacat ttaacatctt
     8821 aatgatacgt tccgtaccaa ttatcccctc ggatacacca gtgtaaaagc tatcgcgctt
     8881 aaggtaatcc atacgatcca tatccagctg tcctgaaata agctggtgaa ggaagtggcg
     8941 tgggtactta tcttcgaata tttcaatggc aagtgaaagt ttgccatcaa actccttgtt
     9001 gagctggtac aaaaagcatc ggcttaagtg ttcatggctg atatcgttag caattacatg
     9061 ctccagggca tgtgagaaag gcccgtgacc aatgtcgtgg agcagaattg ctacggcagc
     9121 agcttcggcc tcgttatcgg aaatgtcgag ccccttgctt cggagtgttt caatagcaag
     9181 gtcgagtagg tgcattgcac caatgcagtg ctgaaatcgg gaatgaacgg ctccgggata
     9241 taccaggtaa ctcagtccga gctgcttgat gtttcgcaaa cgctggaagt agggatgttc
     9301 aataaggtca aaaattaatg ggtggcttat gctgataaag ccatgaaccg gatcgtttac
     9361 aatttttttc ttattagtcc cattcatata aaactatcac cccagagggg tgatatattg
     9421 ctataccatc tcaaaaagga ttagccttta atttcaacca aattaaaatc caccttgttt
     9481 attaaagctt tatagtcatt cccagcaaag taaatatcct catcatcctc ctcaaaatac
     9541 cagttaatgg taaccttttt cccagtgtca actgcaagct tctcaaactt tttcatgata
     9601 tcaaaaatac gttttgagga gctggtgtta aagtactcaa acttcaggtg tacaattgtt
     9661 tcgggttgag gattagttgc atattcatca atccatcgca gaatgggttg gtaaaagtcg
     9721 attacatttt caggaatcga tcgtccctcc atgcgaataa caccggtatc gggatcgaag
     9781 ttcacgtatg gtgttgttgc cgtttctctc aactcgtact tttgcataac caaaagtttt
     9841 atcactaaaa aaacaaatat gcaaaaaaat tagatactat gaaaattcct gtaaaatttg
     9901 gtactcatcg ccatggccat ttctaactat tgcatcccag tacctatacg aaattatggc
     9961 ctcagtgccc gttttcttac tccggatttt gacctttttg aagcgttcct tttccacatc
    10021 aaccccaata aactcaaaag gcagttcaaa tattgacgca aaatcgtgac ctaactgaag
    10081 aatggcatcg tcttcaattt cgtgccgcca ttttactgat acctgtgttt tgacgccatg
    10141 aatctcttcg agtttacgaa aaatctcact tatgtactta tttgagctac tgttagaata
    10201 ctctaaaaag ataatcactt gggtatttgg cgaaggtttt tggcaataac cttcaaccca
    10261 ctccaaaagg ggttgccaaa aggtaaaagg atcttcggta attgaacgac cttctatttc
    10321 gagaattccc tcatcggaaa aatttacgta cggacaatcc tgtgtttttt caataagtat
    10381 tcccattcct atggtatttt accatgccaa ttaaggacta ttgtcaaaag tattaaaaaa
    10441 aaagatctaa aacaaatgca tttttatgaa catcaacttt tctcaggtta aataccccta
    10501 gcaaaattaa taatagccct aaaggaaaat aaccttagtc caaaaactac aagtaaactt
    10561 gatgcaagag ttgctatgaa tcccgttaag ttattaccaa cggagtattg aatcatcaaa
    10621 ccaaaagccg agtaaattaa aatgtatagt gatattttta gcaccgatgt tttgctaatt
    10681 gcactccctt gactacgaat aaagaaaaaa gcctgcagta aagatgctat tgcattagca
    10741 acaaggttag ccatggctgc accaatcacc ccaaaacgcg gtataaggat tagcatggaa
    10801 aaaagggtta caattacagc accaagcgat attctacaaa gcaaccaaat tttccccttg
    10861 gctgttagag cagttccgaa aagatatgat aatgccacaa aaacaaaaga gcccattagt
    10921 aaaaccaatg ctttagatga aacatcgata tgctcatggt ataaaacgct cattaccggg
    10981 cggttataaa tcatagttgg aactgcaatg gcaaccacag gaattagaat caggttaaac
    11041 gaaaagttaa ataattccgt aatatcttcc tggttactca tcatgcgcga gaaaattgga
    11101 agcataagcg cagcaaacag gtatgagtac atgttaaatg catcaaataa cctaaatgcc
    11161 tgagcataaa tgcctgccga aactgctccc tcagggcata aacgttccac cataactgta
    11221 tcgagcctac tgtagagtgt cataagaaga accagcgcgg caaatggcaa gcccttttta
    11281 atcagcgtta ctatagtaat cctgtcaaac tccctgtaaa ttgcaatgct atatttccct
    11341 tgaagcaacc aaattgcaat aaatattgca actacataag ctagggtttg tgatagtgca
    11401 aatacctcaa ctgttaattg tatattaagg tttccccata aaagtaacga taccaaaact
    11461 ataacaattg ctttatccat aacacttaac acgctatcgg ccttaaaaag atgtaagcca
    11521 ttaaggtttg atcggataaa aagtaatagc gatgctaaaa attggttaag tgtaagcacg
    11581 gctaaaagga ctatatgcct tttcccaaaa cccaatgaaa ccccaatacc caatgtaacc
    11641 ataaaataca ccaatgccaa caccaacttt attaccaaca catttgcaaa ctgttgcgat
    11701 atcagctgtg ggttatagct gatttcgcgg ttattgaaat tggttatacc aagatcgagt
    11761 aaaaaattga aaagaagtga gaaattaaag agggcaaagt aaaaaccata ctcctgagca
    11821 ccaagtacaa tttgcacatt gcggtcaatg gcaaatatcc aaagcggctt cactaccaca
    11881 tttaaaagca aaaggaaaat taaattggtg gcaaataatt ttctcattta tagcaagtat
    11941 agtagcgcaa gatattttaa atgtagcaaa atgcgatatg caaagctcaa aaacatctgt
    12001 ttttgttaaa aaaaattaat tgtgttttgt tgtttacgtg aactttacta tatttgcact
    12061 cgcaaactga attgcggggt ggagcagttg gtagctcgtt gggctcataa cccaaaggtc
    12121 gcaggttcga gtcctgcccc cgctacaaag aagtaaaccc cgccaatggc ggggttttat
    12181 gttttaattc cctacttaac cgataattcc aatttttacc gcataaactc ctgttaattt
    12241 attcactcgc aggttcgagt tcccgcttgg cgggactggc tctgcccccg ctacaaagaa
    12301 gtaagccccg ccaatggcgg ggttttatgt tttaattccc tacttaaccg ataattccaa
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggactggct ctgcccccgc
    12481 tacaaagaag taagccccgc caatggcggg gttttgtatt tttaatcctc acttaactgc
    12541 taaatccaat tttttaacgc ataatctcct gttgattttt tcactcgcag gttcgagttc
    12601 tcgcttggcg ggactagctc tgcacccagc tgttaatagg atgaaactaa acaaaagggg
    12661 aactcaattt gatttaatgc tatggcttag ttcaccaata aaagagtttc ttaattaaag
    12721 cctttgtcta taaatattag ctgttgatat tcatttattt ctgtactatc aaaccaaaaa
    12781 gtgatcttat caaattccag acccaacaga ttgacaaaac atagaatatg cattaacttg
    12841 accaaccaaa caaaaccaac cacaggccat gaaaaaaatt ctatttgcac ttattttgct
    12901 cgcaagccaa acagtacttg cacaggaaac tgtaaccata aaatttatta gaccatcaaa
    12961 ggttcaaggg tcagctgcaa aaatccgaat tatcattaat gataacgaat atgttcttaa
    13021 aaatggaacc gaaaccacgg taattattcc tcacgattac agcaaacctc tactagttga
    13081 agcatcggct ggtggtactc gtcaatcgta cagattgaga gctattccta gcgagaagta
    13141 tgtgtttgaa acaggttttg agttcaaagg gatttacctg aaactccttg aggggaaaga
    13201 gcttgcagta ggagaagtgg tacataaagt agatactgca aaagcagggg gtgaaaccac
    13261 tctaaaagtt gataaagacg ggcgagcagt gggatttacc attgagcgga ttgatgagtc
    13321 ggaggccata aggcaggagt ggctttcacg gggtggaaga attaaaaacg agtcagtcct
    13381 tttaactgga acttacttcg gaatgaacct gaaggatttt gacacccgcg ttgatggtta
    13441 cggtgggggt atatcggcct accagaactg gattaacctt aaaattccag agttcaaatc
    13501 gggaatttca acctggaaca gtttcaacta cggatggggc attgacatgc tgctgtatag
    13561 gtacaaatta aatatggacc tttccccatc gggaagtatg gatatgtcgg ttcttaccct
    13621 atcgtatccc atctctgcaa accttggttg gacttttggg ataggcaagt ttcttgacca
    13681 aggaaactgg aaaggggtag cactaaccct taagtataaa ccttcattaa cgctaaacat
    13741 ggtaagcact acctcaacca taaaaatgcc agcaccacta aacaccaccc aaaccgatta
    13801 cagcagcgat acaaatgcaa gcatcaacct aggcggtttt ggcgttgact ttgaattttc
    13861 aaacttcacc gcaacaatga ataggttagc ccccacccca aaaactaagt tctcattctt
    13921 tgtattacca ccagttggcg acacaccgct atttgtatca ataagtttag gtatctcgtg
    13981 gtactcaagg tagtttgagt aaaaagtata ataaagaccg aacaaatgtt cggtcttttt
    14041 ttattacacc ctaaaagtca aaggtatagt caaaaaaggg aataattttc tttaactttg
    14101 gttgctaatt acaacctcaa tgcttcaaaa aatacaaaac agaaattact ggattaagcc
    14161 aaaggtaatt ggtggtttct tactgataat tattttttcc gttacagcaa ttgttatgac
    14221 ctaccaggcg gttagcgatc taaaaactac ccgccaaggg cttgggcttc ccggacaaaa
    14281 aattactgtt ctgaacaggt tgcttgcatc aatttatact gaggatagcg attttaggat
    14341 gtatgtactg acctcaaacg acgattacct taaaacctac aacaacaatc accaaaaagt
    14401 tactgctgca ataaaatcgc ttcaaaaact ggcatacagt aaccaatcgc agctaaactc
    14461 aataaaaaag attgaaaagc tacttcagaa caaaagggaa attgagggcg aaatactaca
    14521 aatgcgacaa agtattaata ccagcatctt ttacgacagg gcaatgcaac gaattgcccg
    14581 agcagggtcg gaaacccagc gttaccatga ggtttacaag caaaaaacag taacccaggt
    14641 aagacgcgat accgtaattt acaaaaaggt agacaatgaa tcgatggccg ccaggataaa
    14701 acgattcttc ctgggaccag aaagaattga cactattgct accgataccc ttgttgaatt
    14761 tacgtacgat accatacctc acatcaccta catatcggac tcaatcattc aacggctttt
    14821 actaattctg gacaatataa ggaatgatca gcagaaccaa cttaggctta tcgacgaaaa
    14881 ggagaaagaa ctgcttaaca ggaatagagc catcctgaat caggttcagc ttattgtaac
    14941 cagtattgag aaacaggaac aggctgaagc tgttagccaa gcattatcgg tacaagaagt
    15001 cttgaccaaa aatctcctaa taatattcct gcttgggacc attacgttta ttgtgctggt
    15061 tatacttata gcaattataa tgagggatat atcgcgcaac agtacataca atgctcaact
    15121 tattgaggca aaacagtatg ctgaaaatct tttgcgtttg aaagagcaat ttctggcaaa
    15181 tatgagtcac gagattcgtt ccccattaag tgcaattgta ggagtttcgc gtcaactatc
    15241 aaaaactgaa ctgaatggca agcaaaagga gtacgttgat gtgcttaata gctcgtcgaa
    15301 ccacctgatg gctgtaatta acgatatact ggactactca aaactagccg ctggcaaact
    15361 acgattagaa agcagagagt tctcgccacg cgaagtggta aacgatacgg ttaaactttt
    15421 tgaggcaagg gcaaatgaaa aagggttaca aattctttcg aatatcgatc aatcgttacc
    15481 tcacattgtt gttggcgacg actttaggtt aagacaggta cttataaacc tgcttagtaa
    15541 tgccattaag ttcactgaac agggcagcat tatagtatcg ggagcagtac tccgcaaaac
    15601 cgacgattcg ttaaaaattc aatttactgt tgcagatacc ggtattggca tctctcctga
    15661 gatgcagtcg caagtattcg aggaattcac ccaggccgat ggcggaatat cacgcaagtt
    15721 tggtggaaca gggctagggt taaccattgt aaagaaacta gttgagctac agggtggtga
    15781 aatatcgctt accagcgaac ccaatgaagg taccattata agggtggtaa taccttacag
    15841 tatcaaggaa accagaactg ccaatgttga ccaagaaacc cattacagaa taaaagctgg
    15901 cacttcaatc ttaatcattg acgacgatga ggttaaccgg ttaatagtta ccgaaattgc
    15961 caaaaacgtt ggcctagagg ttgatgccat aagcgatgcc aaaaatttaa ttgatcagct
    16021 taaagcaaag aagtacgatg cagttataac cgatattcaa atgcccgaag tttcaggcta
    16081 cgaggtggtt caactaattg aaaatgctgg ttatagcatt ccagtttttg caattacggc
    16141 aaacgatatg attgacaatc ctgaacattt tagcagtttg ggattctccg ggtacctcat
    16201 aaaaccattt actgaagcag accttattcg actgcttggc cctgtaattg gagcaactgg
    16261 ggttgttacg ggcgataaaa gagagagggc tgatacttac aataaattcg atatcgagga
    16321 gatataccga tttaccgggg gaaataaaga aagcgttaag ctaattttag agtcgttgct
    16381 tgaaaacacc cgaaaaaatg ttgaagagct aaatcagctc attaaaagca agaatttaac
    16441 taaggcttca gcggtagccc ataagatgaa atcggctttt aaccagttta gggtttacga
    16501 catagcaagt atacttcaac gaattgaaac gctccccccc gacaaacaca aggcagccac
    16561 actatacatg gataggctaa accgaagagt aaagagtttt acagttgagc tgcaggaaca
    16621 aattgacaac ttattgaact agacaatccg cactcccata acaacaacat cgtcgattct
    16681 tggggtatta ccatgccagt tcttaagagt ttcgtcaagg atttggcgct gttcactcat
    16741 gggtttatga tggatttcaa ggagaagttc tttaaagtgt tttatcataa acttacggcc
    16801 atcttcgcct ccaaactggt ctacatatcc gtcggagaaa gtataaatta catccccctt
    16861 ctggatttga ataacattat tggtaaacga aagttcaccg cgcaggtgaa tgccaatagg
    16921 cattttatcg cccttaatat gggtcaactc gttgttgcgg atcagaataa gcggattgtt
    16981 tgccccggca aactgaaggg tttgggtttc ctcatcaata atgtaaatgg caatatccat
    17041 cccatccttg cttccgccta tttcaccggt ttggtgaagt gcagttataa ccaagtttcg
    17101 taactcatta agcacttcat ttgctttcag atcggttttt gtaccaatga tctgggttag
    17161 gaaggatatg cccaacatgc tcatgaagcc accgggaact ccatgtccgg tgcaatcggc
    17221 aaccactgaa acctttaact tgcctgcctg agtgatgtag tagaaatcgc cgctaactat
    17281 gtcacggggt aagtaaagta taaagttttc aggaaagatt cggttaatct gatcggtggg
    17341 tgtaagtaat gctgtctgaa tgcggcttgc atactgaata ctactggtta tctcctcgtt
    17401 ctgggcagca attttttcct tttgctgttc tatttcttcc ttttgagcaa caacctcggc
    17461 cgttcgttcg cgaacaattt gttcaagacg ttctttttca gcaatgagtc ggcgggtatt
    17521 ccatttaact ataccccaaa ccaaacaaac tgcaagtata aaatagagta tgaatgccaa
    17581 aatagtacgg taccagggcg ggcgaatttc aaaagagtag gttgcctcta tgctttcatc
    17641 cccataaata ttctttgcct taacatggaa tgtatattta ccctccctaa gattggtata
    17701 agtagcatcg gttcgtttat cccactggtt ccaagtggtt tcgtcagaac cttcaaggta
    17761 atggctatac actatatcct cctcacgttc gaaatatgga gctgcaaaag taaacaccat
    17821 ggcattatag tggtaaccca atcgcggcat ttggtaaggt tgttgctcta acgataattt
    17881 tcgaaccgag gaatcccctt gagtataaaa ggctccaccg aacagtaatg aatcctttga
    17941 taccacttcg cgaattacgg cacggaatgg agcgttaaaa cttcgtttta catgaggatt
    18001 gtaaacgtaa agctcttttg acatgccaat ccaaagggtt ccgtcaggat cagcatatat
    18061 cgcatcggcc cacttattgg gaagtcgctt tagatcgcgg ttgtcagtta cccagttccc
    18121 agcagaatcc ttaacaactc gttcaatcca gttcttattg ttactatcgt agcataggaa
    18181 ggcataacca ttggagaact ttgtggcttt ataaatccca aatcgctttg aaaaagcgct
    18241 acctactaaa cctcccggct caaacaggtt gttttcatag ttaaagtggt atattccatt
    18301 tgaagtaagg atatatagtg aatcattaac agggaaaaga attatatact gaaggttttc
    18361 tagccccttt atgctctcct tgccaaactt agataatttg gtaaaactct tgttaaccat
    18421 atacacacca gagtaacctg tggcaagcca aaggttccct ttactgtctt caacaattga
    18481 gcgtatttca aaattaatgg aatcgcgaaa taagttatca gtgtgtttcc attcgccgtt
    18541 tgcccagtct atatatgcaa cacctgaatt taacccaata tacaatctac caggtatgct
    18601 tttagatggg tataaagcaa aacaactatg ttgaatttgt tttgataatt caaggtttgc
    18661 atttaaagga ttgctaatac ttatggcttt acttcccttt atttcaaaaa caccatcggt
    18721 atagctgcct gcaagaagca ttttgggtgc atttggtggt gaaaaaatag ttaacgccca
    18781 tacatttcca ttaataccat ctacaggtat aaactttgga actccattgc tgtcgtaagc
    18841 caaataataa accccgttga aagtagcaag ataaatagta ttattaaatc taacaatgtc
    18901 aacaataatt tccgataagc cacactcctg accaaatctt cctatggcag agttcaactc
    18961 aaccttggca ataccaccat cgtatagtgt tgcccaaagg ggtgcatcgc cattttgaaa
    19021 cacttcggac acttggcctg cgtgcattcc cgtttgtttg ttaaccagat ttatggtttt
    19081 taagtccggg gctagcgtaa caactccaaa aaagtcggat tgaacaaccc ccaaagcaat
    19141 attgccatca ttaagtttag ttgcgctgta tggtattgct gactcggtgg ccattctttt
    19201 ggcaaggagc gttggagtgt caacgctttc gaccttaccg tttgaatagg tataaaaccc
    19261 attatcagta aaaataaaaa ttttgctaga gtcaattgct acaattgaga atacattttt
    19321 atgaataaaa gcctgaccac cggggataac ttcaagggta ctgtcgcttg taaaagtcct
    19381 taaaccttga agataactgt taacataaaa cttatcgtta gcctttaatg taaaaaagtt
    19441 agcatattca ctttgctttc ccaatgaaat aggttttata gtattaccat cgaaaataaa
    19501 aatatttgat agagagcaaa agtaagtctt accttcatac cagtatgttt tgtaaatgta
    19561 agaaaggtta gactgaatac tatcgggtag gtttttgata agtgaaaaat attgtaacct
    19621 cccgtttgca gtaggctgaa ggtagccaaa ctcacctgtt gaaccaacat atataacccc
    19681 atcggcaccc ttacaaagcg aacgaaccga cttgttttca ggcataggga tgctacgcca
    19741 ggttttgcca tcgtattcca gaatgccatt atcgtttgta ccaaagtaca tcacacccct
    19801 atcgtcctgt gcgatacacc aaacctgctc gtttccaccg taaacctcag gggtatagtt
    19861 tgtaataagc ggattgccat acttattgaa ttgctggcca ctcgcttgta aaacacttat
    19921 gaacaaaata ccaagaaata gaagcttttt catggtaaaa cgctatacta agatttgata
    19981 aatatattaa aaaaacgttt aatcagtcaa tatttttaac taattgctcc aattagtttc
    20041 ttaaaaaaga aaaatcatat ttcaaagttg taaagataag gtttttaata atatcgaata
    20101 gaaatctacc aatttcacta ctgtccgatt taaaaaatct ttatccacta agcgggaata
    20161 ggataacgtt acgccgttgg cgatgaagta ttgctagaaa aaccaacctt aacatttcct
    20221 attcccaatg gaggatatag aataagttag gtataagcta actcttaaaa tttttttagc
    20281 caaaaagtag tgattatcta tacagaatca atcgccatta attgggtaaa agcaaaaata
    20341 atgccggtcg ttgaccggca ttcacaaaaa agtaaacgat tactgcttta ttaacttttt
    20401 taaagtttcc agttcagcct ttaagttctc aatttccttt tgctgctgaa taatttgcaa
    20461 ctgaaggttt tcaacggttt caaccgtttc aaactgcatg cgggttaggt taatgccatc
    20521 cctttcctcc gatgcttttg ttagccaggg taagtggcca ttacgagcaa tgaaccgttc
    20581 aacgctcagg ggatcgagcc gcttggaata atcgggtttg aatacaaagt ctggtttaat
    20641 aacgtcatta caataaatat tgcctgtaat tcgtgcatca cccaccacgt gtagctgttt
    20701 atcgggcacg gttgttccta tcccaactcg ggcatttaaa gcaatttctt tgctatcgaa
    20761 tctgccataa attagtgctt gcgttgaatc gcctccagaa ttggaaatat gcagtctatt
    20821 acttttagcc tcggaaatac cagcctgata tccaataaag acgttgctgt tccctgaaca
    20881 gtttagtcca gcctgatgcc caatgataac attagcaaat ccgttatttg ttccagtttg
    20941 ctgtccaata aaaatattgt aaaaaccatt ggttaatccg gttccacttg catacccgat
    21001 acatacatta ctactagcaa taaaaggacc agtgttgaaa tttgcagcgc tttgagtacc
    21061 aataaaggtg ttgttgttgc cattaatatt gtaccctgta aaacgcccaa ccattacatt
    21121 gcccgatcca ttagtattac cccgaccaac attggagccc aaaaaggtat tatctcttcc
    21181 tgtatcgttg gaggctccgg atgcctgacc cacaaaagta ttttcatttc ctgatgtatt
    21241 gctagaacct gccatatcac caagaaacga gttgtcagaa ccatcaacat tcttgtagcc
    21301 tgaccgataa cctagaaata ggtttctgaa accatttaca ttatagtagc cactctccaa
    21361 accaaaaaat gtattgccaa aaccagaagt gtttgaaaag cctgaaccgt ttccgaaaaa
    21421 catatttcgg tttccatcgg tattactttt accagactca ttaccgataa aaatattact
    21481 gtagccactg gtgttgctat atccggccat atagcctata aaaatattct taaatcctgt
    21541 ggaagtacta aaccctgatt gttcgccaat tgcaatattt tgcacaccgt tcaatgcatt
    21601 tcctcccgcc ttactcccta agtaaacatt accacctcca aattggtgaa gcattcctgc
    21661 ctggtaacca atgaatgtat tatcgcttcc ggtattattt aagccagtct gatacccaag
    21721 gaaggtattc caattcccgg tgctgtttgc tttcccggcc tgaaatccta aaaaggagtt
    21781 aaactgtccg gtagtaatgc ttgccccagc ttcgtatcca atcaggtagt tatccttggt
    21841 tagctggatg agttgagcac tagttttccc gttggtgagt ccacccacgg caaagccacc
    21901 cttggcgccc ttactcagat tctcgttaat gtaaaaccta gcagaatcgg ggttgacaaa
    21961 cagcaggtct tgggctataa ccgctttggt ttgattggtt agtccaccca cggcaaatcc
    22021 gcccttggca cccttgatgc cggtgctctg cttaacatat accctggcgc tatcgggggt
    22081 tatacgcagg tactctaccg gctctccctt tgcgccggtc aggccgccca ctgcaaaccc
    22141 acccttggct cctttggtta tggcgctgtc ggggatattt acccttaccc cctcctggta
    22201 cacaccaaaa agaacctcgc ccttgctgtt ctttacctca aatatggggt cgtcggggtt
    22261 ggcggggtcg ttgcccacta ccactaagtt gcgcgaaaag cgcaggttaa tactatccgc
    22321 ataaccagcc cgagccgcag aatcggcgaa aagggagata gtgctctcgg ccgatcgata
    22381 cgacacccta gcgctgtccg agaagatagt atacggtaca ggttgtattg ctttagatcc
    22441 catatcacta tagttaaccc catcggtgct cacctccacc cgcagccgga cttcctgccc
    22501 ctgccacggt accgcgctgt aggttccgga tactacagtg cccccgccca cggtgaggct
    22561 caccacaccc tgggcggtgg tctggagcgc atggctctcg gagtagtacg tggttgtgcc
    22621 gctggcatcg gtcaggctca gcctgagggt cacgttctgg ttggccagca cctgcccgct
    22681 gctgttgcgg agcacggcct ggtagctgaa cccaacgggt tgctgcgctg cagtagttat
    22741 tgcaaccacc aagaaaagca gcaaaacctt aagtttattc attatctttt caatttaggg
    22801 ttataactaa aaggcagggg aaagtttaat ctcacaaaag ggaaagctta aaatatctta
    22861 taaaaaagat tcaggggtga gcctctgcat tgctcacccc tttgaaaaat catttattct
    22921 gttttactac ttcaatcaaa agcctttgta attcattaat ctgctcctga aggttgttta
    22981 tttgctgttg ctgttcttta atcgattcta ttaaaaccgg aactaactta acataatcga
    23041 ccgacttaac cccattttca tcggtttgaa ctagctcagg aaaaactttc tcaacatctt
    23101 gagctatcac gcctatctgg gttccaacgg ggaaattgaa tttttcaatc cctttattag
    23161 actctttgtc ggaaacaatt ccctttattt cctcattact cttccattca aaggtaacgc
    23221 ctgtaatttt actaagcttt gccaatgaac ctttcaatgg agtaatattt tttttcaatc
    23281 tctcatccga tggatttgta aatgttccag acacataaac atttccggaa aaataaccag
    23341 catagttggt agttccattt gcaccatagc cataaacacc gtatcgtgtt cctgtgccgg
    23401 ctacacttgc tacaccgtat actcccatat atccaccatc gccttttacg cctatacccc
    23461 aaccagtagt gttaagttta tgttcaccta gtacagcagg atcgtcaact gcaatagttc
    23521 tatcgtcata agaaaaaagt ttggaacctg tgggatcaac attaacccca acatttgcat
    23581 taaactttaa ctttttattg tcaaattcac catagattaa gggagtggta gggttaacat
    23641 cggtgccaat aacaagagca ttagatacgt tctggaatgt cccatagcca attttgtatc
    23701 caataaagac attctttgac ccggtgttac tccaaccggc agaggttcct aagtaaatat
    23761 tccctgaacc agttgaattt attgaaccag cattagtacc aattattaca ttctcattac
    23821 ctgttgttaa cgaactacct gctccaccgc caatcaatat ctgctgatgt cctgtggtgt
    23881 tgtttttccc tgtccaccta cctatcataa tattgaagta accttcagta ttattctcac
    23941 cagccgatgt accaatgaat atattttggc ttgaggtatt cttaaatcca gctaaatcgc
    24001 caatagcaat gttattttgg ccagttatac aagagtaacc tgctttatta ccaatgtaaa
    24061 cattattata gctgttgttg aagcgacctg ctgcatagcc aatgaacaca ttagtggttg
    24121 cataagttga tgatgttgag tacccagtgc tatcgccaat aaaaacattg tactgaagat
    24181 tagtacctgt gtaaccagca gctttgccca aaaaaatgtt actaacacca gtttgatttg
    24241 aataacctgc ataatcacct atagctatat tagaatttcc agtagtatta ctccgcccac
    24301 tctgattccc tataaatacg ttgctattac ctgtagtatt ggctctacca gtataattgc
    24361 caagaaagga gttgttagtg ccagtggtat tatatctgcc gctttcgtta cctataaata
    24421 cattattacc agctgatgtg tttgacatcc cggccattaa gccaataaaa atgttattgg
    24481 aaccatcaac atttgcatac cccgactgga agcccaaaaa ggtattataa gtacctgaag
    24541 tgtttgctcg accagcttga tagcctacaa ataggttgta gcttgcagta tttgagtaac
    24601 ccgcatcgta accaagaaag gtgttgaaat ttccggaaat attgcttaag ccgctgctag
    24661 tacccaaaaa aacattattg gaaccagttg tattattaaa tccacttcct ataccaacaa
    24721 atacgttgct ggagcctgaa gtattggctc tacccgttgt tgttcctaga aaaacattgt
    24781 agtttccggt tgaagttgag tatcccgact caaacccaat aaatacgttc ttaaaaccac
    24841 taactatgga gtagccagct gattcaccaa tgaaaacgtt tcgttcaccg tttgtggcat
    24901 tggaacctgc cttgctccca atgtaaacat tcccaccctt ggtggtatgc gccatacccg
    24961 cctggtagcc aataaatgta ttatcgcttc cagtattatt caaccctgcc tggtaaccta
    25021 ggaaagtgtt ccagtttcct gcattgttca gcttaccagt ctggtagcct aggaaagagt
    25081 tgtatagccc cgtggtaatt gacatccccg cttcatatcc aataaggtaa ttatccttgg
    25141 ttagttgaat caactgcggt gacgatgcct ttgtctgatt ggttagtcca cccaccgcaa
    25201 aaccgccctt agctcctttg gtgggattct cgttcaagta tatcctggcc gagtcggggt
    25261 ttataaatag taaatcctga gcaactaccg ttttagtttg gttggtcaag ccacccacag
    25321 caaaaccacc tttggctcct ttgaccccag aatcctgctt aatgtagatg cgcgcgctat
    25381 ctggggtgat tcttaagtac tccactgggc cagcttttgt ttggttggta agtccaccta
    25441 cagcaaagcc acccttggct cctttaatgg cttcatcccc aatgtttatg cgtacccctt
    25501 cctggtaaac cccaaaaatt acctcgccgt tgctgttctt cacctcaaat attggatcat
    25561 cggggtttgc tgttgggtta cctaccacta ccaggccttg ggcaaatgta aggtttggca
    25621 ggtttgtgat gctgcttgca ccattccaca ccgccatctg gccgcttacg ccgcttcctg
    25681 tcacccagct aggggtttgc cataccaccc cacttgtccc tctgctaagg tactgccccg
    25741 atgtaccgga actattaccg taatcaacaa gcaatccccg caacctcata tttccattaa
    25801 cgtcgaggcg ctgggttggt gtagtggtat ttataccaat atttgtggcg ttgttatata
    25861 ttagactact ggccgcccag gacgtcccgt cgtggcgtag ggtttgacct gtagttccgg
    25921 caaccagggg tcccgggggg ccctgttctc catcctgtgc cagaatttcc cacgaatcgc
    25981 catcccaaat atatgctttc ttagcaacag agttgtagta ggcctggttg gttactggcg
    26041 aggagggggg ggcaggaagc gttcctagcc actgtatcga aatgccattc tgtccggcag
    26101 gaccggcagg ggcttcaaac tctacccagt ggtcgggatc aagacgcggt tgcagggttg
    26161 aaagataggg agaagtgcct tccaatatcc acatgctgtt agccgttcct cccgtgttca
    26221 atgcaaacac atagtcaccc ggatcgtatg tggtcccgct tacccaatcg cctcgattat
    26281 ttaaacccgt acccgcaggc ccctggggtc cttctgggcc ctgaggtccc tgagctcccg
    26341 gtaatccgtc ctgagcaaga atctcccacg tatctccgtc ccagatgtac gatatcttgc
    26401 tggtggtatt atagt
//
LOCUS       DAOOWJ010000028        25114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_1986_length_25114_cov_8.463347, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000028 DAOOWJ010000000
VERSION     DAOOWJ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 25114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25114
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1986_length_25114_cov_8.463347"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..909)
                     /locus_tag="PLW22_04410"
     CDS             complement(403..909)
                     /locus_tag="PLW22_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOU73981.1"
                     /translation="MEYRGDPLVYQTLNELSIPFEYYEHPEAPTVEIASKYWEGINSS
                     HCKNLFFRNHKGNRHYLVILEYRKMLNIHQLEKLLKQGKLTFASSERMKRYLGLTPGS
                     VSPFGLLHDTSRHVHLFIDSELKNTQRLSFHPNLNTASLVIPTIDFFRYLNWVGNTYE
                     FIDYVNEL"
     gene            complement(914..1420)
                     /locus_tag="PLW22_04415"
     CDS             complement(914..1420)
                     /locus_tag="PLW22_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOU73982.1"
                     /translation="MIDVKLSIVNSKPEIFRIIRVDENINLQLFHEVIQAAFEWSNMH
                     SHFYNADKSILKNEEEILLGNALNKWESLIYVYDLADFWTISISLLATNSGELSKKPV
                     CLQGQGKSPPEDSGGIINYCEVLELLAKHNKGDRQYPLIADWLGENYNPNEFNCEQVN
                     LNFKNLVL"
     gene            1653..3245
                     /locus_tag="PLW22_04420"
     CDS             1653..3245
                     /locus_tag="PLW22_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73983.1"
                     /translation="MIRFLKDINLRLKLLLVLIVLTFLYGFYIDFFEGPKPIHEWRKA
                     DCLSITYNYMKGEPFFEPKTNLILENGNRNAVSEFPIIYFLMGKLWSVIGQNEFLYRL
                     LSFVLLLLGIILFATVVQYYLKSQKYTIIYSVFMFSSPILIGYAHNFIPNPISFALNL
                     IAGFFVFQFLQNNKLYGLIGFFVFSTLAILIKITSILVFMSFIGAFIFYELFDNNYFW
                     RKYKIKISLLSGSFILTLALVAAWYYYAIYYNTKYDSHLFSTTIRPIWEIPAEKRNEI
                     FWHIINTMINYAYHRVIVFALIIFGVVALFSRSINAFLKWLIVMSIGALFSYLILWYW
                     VFDVHDYYLIEILFVFNVLFFIFLYKINSFFSFLDLKKINLALYFFIIFVVLHAYTCS
                     WVRFSANPKLIVNNIFLKKRDIDTWRWFNYDYGINLKTLQSKSDEIKKIIQPSDTILC
                     LRDYSPNVHLYTLGRIGYSKFLYNGFSDREAIVDAYKRGARYVVFLGSEEERAKIPYS
                     DYVAYQYERIYIFDLKPYKKIQ"
     gene            3242..4162
                     /locus_tag="PLW22_04425"
     CDS             3242..4162
                     /locus_tag="PLW22_04425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU73984.1"
                     /translation="MKISVVLPCYNEEGNIKLLYSELRSVLTDKYEYEIIFVDDGSVD
                     GTLSTIKEIAMDDRKVKGISLSRNFGHQSAIIAGMNSASGDYIITMDADMQHPPTLIP
                     TLINKANEGYDIVLTIRKSTVDAKFIKRITSHLFYRFFNYLSDIPLKEGSADFRLMTR
                     NAANVFLGLPEKVRFNRGLINWMGFKTAFVEYHSPRRISGKSKYTFIRMIKFAIAGIL
                     SFSSKPLKIPFILGLLSFIFGLGYSIYVLVQFINGMTIPGWASTIILILMLGGAQMLS
                     LGIVGLYIARIFEETKQRPIYIIKERINIE"
     gene            complement(4228..5379)
                     /gene="galK"
                     /locus_tag="PLW22_04430"
     CDS             complement(4228..5379)
                     /gene="galK"
                     /locus_tag="PLW22_04430"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOU73985.1"
                     /translation="MVERIVSTFKDRFGSDPFVFRAPGRVNLIGEHTDYNNGFVMPAA
                     INRYAYFAIKPNGLGLYRFHAADFRRDFTTPVYKIEPSNAHWTNYLLGVIAQMVAEGK
                     DIPGFDCVFGGDVPIGAGMSSSAAIESGLAFALNHIYGLGFTRMQLAQIAQRAEHEYA
                     GVKCGIMDQFASLHGKQNHVLLLDCRSLEFEPFELNMDNHSIILVNTGVKHSLASSEY
                     NKRRQECQAGVDILKKYNNSIQSLRDVTINDLNNHKREFSSKVWERCLYVVEENQRVV
                     NAGKALTQKDFESFGKLMYQSHYGLRDMYEVSCPELDKLVELTEKLDYVVGSRMMGGG
                     FGGCTINVVKNAHIDEFTKCVKENYKTPDGKIPQIITCVISNGAELLVY"
     gene            complement(5384..7348)
                     /locus_tag="PLW22_04435"
     CDS             complement(5384..7348)
                     /locus_tag="PLW22_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOU73986.1"
                     /translation="MKRISILTALIFTTDLLFGQSYTLKSPNGILVANIDLKDRLTYS
                     LSCNGNAIVEASGIAMRTSAFAESDFLGKHTATFNSKNQAVKLLWGTTKEIDENFNQL
                     KLNYSNGLSVEFRCYNEGFAWRVVSAFDREVIVYNETSEFNIPNSATVYFPEVKGFLT
                     PFEVNYLPKSMQTIADGTLGLTPFMYKTPSGELVVISESNLFEYPGMFIRKDAGNSVK
                     AVFPLYPKKEKQELPGRLHLVKWPQISRMVVKKTENYIAKSNGNRSYPWRVVMVAQND
                     AEILQNSLVYLLADEPKMDYGWVKPGKVVWDWYHNWNLQGVEFKPGINTQTYKYMVDF
                     AANNGIEYINIDDGWAKLWDFKKVNPNLNLKEVIDYANSKGVKVFIWAMWNTIDKDFT
                     ANLDMFSKLGVAGIKVDFFDRADQRIVDFVNKLADECAKRNLLLNLHGMYKPTGINRT
                     FPNIVNIEGVLGLEYNKFSDKCTPVHNLTIPFVRNVVGPMDYTPGSMRHVTPEQFVKS
                     WENPHSMTTRAQQMAMYIVYHGGVQMLADSPTLYQKDSIAQNFLSGVPVTWDKTIALE
                     GKVGESIAVARKHGDKWYIGCMTANQEHDFNFNFNLNLNLLDDREYKMTIIAEGNSDD
                     ELVLSSKRIGKNDRQNFKVKPRSGLVIILE"
     gene            complement(7372..10485)
                     /locus_tag="PLW22_04440"
     CDS             complement(7372..10485)
                     /locus_tag="PLW22_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU73987.1"
                     /translation="MLQAQNWQDPKVFAINRLPSHTLLVPFPEKSSSISYIFNSEFVQ
                     SLNGDWKFMLINNPLNTPENFSNVEFTDSEWSTIPVPSNWQTKGYGTPIYSNQIHPFP
                     ANPPFVPNEGNETGLYRHTFRIPENWNSSRVIIHFAGVQSAIDLWINGHYVGYSQGSM
                     TPAEFDITPYITAGQNLLAARVINWSDGSYLEDQDFWRLGGIFRDVFVYAQPQNALWD
                     IEVNTSFSKSYTEATLSVSGVLTNTTSQLPNISLSLFDSNGIAVFEKTVQPKLSSGIA
                     TFKVDEIVNNPQLWSAETPNLYQLIVEIEGRYYQQLVGFRDVKVENGQLLVNGQPIKI
                     KGVNRHEFDPYNARTVSYELMEKDVVLMKQNNFNAVRTAHYPHHPYFYELCDRYGLYV
                     MDEANVESHYLWQYRNQSPVLYPEWKDAIVDRGVSMVMRDRNHPSVIIWSLGNESGDG
                     PNMVAMADTIRKIDAAKRPIHYESKALKRPLSFDGVGAFEKLRRMLSALQWSKALTEY
                     DFNAGMYPTLDRLKEMAKLDKKERPILICEYSHAMGNSNGHFKEYWDLFESHPRMVGG
                     YIWDWTDQGLIKHTSNGKPFYAYGGDFGDKPNDKDFCLNGVTFPDRSLKPAMAEIKKV
                     QQYIKFTGIDFDRKTVNLKNTYAFLNLKSFNLTWSISEDGITVQKGELPLPDVKPNGE
                     GSVTIPYTLPQINPGKRYHLNLSVQLANDEPWAKKGFEVATYQYEMPWLVQKPTTIAA
                     TSGNLNVTENADTWIVSGQAFSIEFNKTTGTISKWKSNGEMVAQNGPRVNLWRAPTSN
                     DIGTGFNPDPRFSFHGKLWQKYGLDNLIIKKSKVTISTTGKGVVVLTHQTLEGNKSRF
                     NAVITHVINPDGVVDINYKLTCSKKFNLPRVGLMLELPKEFSQVEWLGRGPQENYRDR
                     SYAAHWGLYRKTVDSMVTPYIKPQENGNRYDVDRVSIFNPNGTSGIEVTGKSFCFSIH
                     PYSLETLTKATHTPDLVESPVNHLYIDLAQNALGSESFFYNYLQEYVLKGKKFEFQVS
                     LKPVTKN"
     gene            complement(10554..11876)
                     /locus_tag="PLW22_04445"
     CDS             complement(10554..11876)
                     /locus_tag="PLW22_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU73988.1"
                     /translation="MRKVIFALLICMVAMPSFAQENQEKKTNEEVKTGWTVGVLPALG
                     YDSNLGFLYGAIVNSFDFGDGSRYPDFNHNLYLQLSAYTKGSMDAIAYFDSYTLIPNK
                     HFIGRLSFNRNRTYPFYGFNGTQTVYNADFENINATDDFISQVFYKYDRKLAKADFLL
                     EDRIADSKFKWIAGFDLGWYDVATVDIDHLNKGRDDDELIPDVPILYDQLVSWGIISA
                     DEKDGGFDNSVKFGLVFDTRDRLTNPMKGVWTELLFRASPKFIGNNDNYGRVSLIHRQ
                     YLTLVKEKLSFAYRLWYEGNLGNVPFYARQHLTTSNYYEGFGGAQTVRGVLMNRVVGQ
                     HTAIGNFELRWKAYRFHAIGQNFYLGFNLFADAGKVLKGYDMDLTNVPVAQRNQLFSN
                     SYKDLYTTFGAGLKIVMNENFVVSADYGVAMDKNYGSSGLYVLVGYLF"
     gene            complement(12039..13145)
                     /locus_tag="PLW22_04450"
     CDS             complement(12039..13145)
                     /locus_tag="PLW22_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOU73989.1"
                     /translation="MTNVIPIYHIGLIQAWFAALLVYRKPNKGAADRLLVIWLIVIGI
                     EMLYSLLNAKYLTWLPDLIIIPLSYGPFLFFYTKLLTSKKPTTLRNIHRHLIPFYLFI
                     LIAAVWGKPIDIDSVNFYSKGPITWLAIFNYALFLIVILYYWRASLILIRKHNRTLGD
                     HFSYRSETIMLNWVKNIAIWILSGFFASGITFIIFSTQNIFPFNPIELFHLGLLVFTF
                     SISYYGIHQPVLYQYQKPKQIEVTEDLQTKTSQKLSDLLYEHMVKNKPYLNNELTIQD
                     LADQLKVSSQEISSYLNKELGVNFFNYINGFRIEEAKQRLTNPNFDNETLLGIAYDSG
                     FNSKTSFNTLFKKETGMTPSEYKKAFRSSTKKNT"
     gene            complement(13212..13284)
                     /locus_tag="PLW22_04455"
     tRNA            complement(13212..13284)
                     /locus_tag="PLW22_04455"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13249..13251),aa:Gln,seq:ctg)
     gene            complement(13386..15080)
                     /locus_tag="PLW22_04460"
     CDS             complement(13386..15080)
                     /locus_tag="PLW22_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOU73990.1"
                     /translation="MTEHELTLGDSGERPKNFLEEIIEEDIRTGKHGGRVLTRFPPEP
                     NGYLHIGHAKSICLNFGLAQKYGGKTNLRFDDTNPVKEDVEYVNSIKEDIRWLGFEWA
                     NEFYASDYFEQLYEWAELLIKKGLAYVDDQSQEEIRINRGTVTQPGKESPWRNRSVEE
                     NLDLFRRMRAGEFKDGEKVLRAKIDMAHPNMLLRDPIMYRIIHAEHHRTGDKWCIYPM
                     YDYAHGQSDSIEQITHSICTLEFDVHRPLYDWFIEKLEIFPSKQYEFARLNLSYTVMS
                     KRKLLQLVQEKVVMGWDDPRMPTICGLRRRGYTPEAIRYFADKVGVAKRDNVIDLSLL
                     EFCIREDLNKRAERRMAVLNPLKVVITNYPEGQVEELEAVNNPEDETAGKRLIPFSRE
                     IYIERDDFMENPPKKFFRLAPGQEVRLRYAYFIKCEKVIKDSQGNVIELHCTYDPASR
                     GGNSPDGRKVQGTIHWVSIPHAVKAEIRLFDRLFSKPDPDDVPEGQDWKSSLNPESLT
                     VIEGYLEPSLKHAKQLDKFQFERLGYFCVDYDSTPEHLVFNRTVTLKDTWAKLAGK"
     gene            complement(15296..15697)
                     /locus_tag="PLW22_04465"
     CDS             complement(15296..15697)
                     /locus_tag="PLW22_04465"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU73991.1"
                     /translation="MNPLVLQASNTTPYIDFNPSENRLTISGYSRPENVRDFYMPILD
                     WLESFSNELKSKMLKEEQIQPIEFQFKLVYFNSSSAKFLYDIIIILNQIQKAKVPLNI
                     NWYYDKDDSELREAGEDLSELAQVPFNFVTY"
     gene            complement(15708..16118)
                     /locus_tag="PLW22_04470"
     CDS             complement(15708..16118)
                     /locus_tag="PLW22_04470"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU73992.1"
                     /translation="MEPLYIEPSEFTPRVFFDPENSVFEISGFSRPENTVGFYRPLIK
                     WLEDYEDNVLAKNTNFNKSNLVLNLKLTYFNSASSKFLMDILMVFMKFHSKGNAVEIN
                     WYYDEDDDEIFESGEEISEMINFPFNFIPITPNS"
     gene            complement(16124..16642)
                     /locus_tag="PLW22_04475"
     CDS             complement(16124..16642)
                     /locus_tag="PLW22_04475"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOU73993.1"
                     /translation="MEKGEVLINHYGPLSYEEIGFLLNKMTSLLERYNFGITIRKKVY
                     SAMVESLENIYKHQDVIKGSNNYQPKFSLILEKDKVDLTCSNSLLKVKIGPLKERLEK
                     VNQLDKAGLKEFYKEVILSGNVTQKGGAGLGIINIAKVTENKLEFTFDDIDEQYTYFT
                     LRIKVSLQNQNN"
     gene            complement(17016..19166)
                     /locus_tag="PLW22_04480"
     CDS             complement(17016..19166)
                     /locus_tag="PLW22_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOU73994.1"
                     /translation="MIKRLTTLLLGFLLLVSSAIKADEGMWLLPLLQKYNIQAMQQKG
                     FKLTADDIYNLNQASIKDAVIIFGRGCTGEVVSDQGLVLTNHHCGYGAIQQHSTPEHD
                     YLEDGFWAKSFEEEIPTPGLTATFLIRIEDVTDQVNAAFKPEMSEADRDKAAMEIGRK
                     IAKQATDGTHYTASVRSFFGGNQYFLLVYEVFNDVRLVGAPPSSIGKFGHDTDNWMWP
                     RHTGDFSVFRIYASKDNKPADYSRENVPYKPKHFLPISLKGVNNGDFTMIMGYPGSTQ
                     RYMTSVEVQERINILNANRIYVRGIKQEIWLKDMQADKAVNIKYASKYAGSSNYWKNS
                     IGMNKALKRLKVYDFKKQQEEEFTKWVLASPERQQKYGEALSLINEGVTGRKEYLHAS
                     QYISEALFSGTEIIGMAAMTRSLVDALKGNKTEEVTKISKSLKARSENFYKNYNTPTD
                     QKTAKAMFRIFMENVPLTYQPDIFNTIRTKYKGDVDKFVDQMFAKTMFTDPAKFNAFL
                     DKPSLKVLEKDPVYVAAASISNKYEEISKVMAPFNEKLQRGQRLYIAGVLEMNEGKAM
                     YPDANFTMRLTYGEIKDYYPMDAVHYDYLTTLDGVMEKEDPDNWEFVVPAKLKELYNS
                     KDYGRYGKDGKMPVAFISTNDITGGNSGSPVMNGNGELIGIAFDGNWEAMSGDIVFEN
                     KLQRTISVDIRYVLFVIDKYAGATRLIDEMKIIE"
     gene            complement(19242..19820)
                     /locus_tag="PLW22_04485"
     CDS             complement(19242..19820)
                     /locus_tag="PLW22_04485"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOU73995.1"
                     /translation="MLKQYLNVNVIEAGCDEAGRGCLAGPVFAAAVILPPDFNDPLIN
                     DSKQLNHKQRMQLRKVIEHYALDWAVASVSNQEIDKINILNAAIHAMHIAIGKLSKLK
                     PEHLLIDGNRFKPYPGIPHTCIIKGDSKFLSIAAASILAKTYRDEFMVNISKEYPLYN
                     WDINKGYPTKKHRESIAKHGTTPYHRLSFRLL"
     gene            complement(19823..20944)
                     /locus_tag="PLW22_04490"
     CDS             complement(19823..20944)
                     /locus_tag="PLW22_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOU73996.1"
                     /translation="MRIAVNTRLLLKDKLEGIGWFTYETLIRITRNHPEHQFFFIFDR
                     PYHNSFIFSNNITPLVVPPPARHPFLWYYWFEFKIPKTLKAINADVLVSPDGYISLKS
                     DTPQVAVIHDINFYHAPQGLPFFTSHYMNYFFPRFAQKAKRVVTVSQFSRTDIANNFN
                     IDPNKIDVAYNGASNSYKPLSESEIQRVRESISEGKPYFLFVGAFNPRKNVARLIEAY
                     DIFRKRTGSAINLLLVGEPMFKTNDIGIAYKNCNHKNDIKFLGRKGLDELTKITGAAH
                     AVIYPSTFEGFGIPLLEAMKCGIPIAASNVTAIPEVTGNSAIYFDPYSVDEMADAMQR
                     VAYDTELRETLSANALNRQQQFSWDNTAQVLYSSIEKCF"
     gene            21146..21613
                     /gene="rimP"
                     /locus_tag="PLW22_04495"
     CDS             21146..21613
                     /gene="rimP"
                     /locus_tag="PLW22_04495"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOU73997.1"
                     /translation="MISKEAILEIVTPLLEKENLFLVDVSVGADNKIIVTVDGDKGVS
                     IDSCVSISRGIENSLDRDREDFELEVTSAGLGQPLKIPRQYIKNIGQELDVVLKTGEK
                     IKGVLNSADNTGFVLGVSKKVNIPGSKKKQEVVEPIQLTYQQVKSAKVVLKFK"
     gene            21640..22878
                     /gene="nusA"
                     /locus_tag="PLW22_04500"
     CDS             21640..22878
                     /gene="nusA"
                     /locus_tag="PLW22_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOU73998.1"
                     /translation="MENLNLIDSFLEFKELKNIDRPTMMSVLEDVFRSMLVKLYGSDD
                     NFDIIINIDKGDFVIYRNREVVEDGAVEDPNRQISLSDARKIDEDYEVGEEVTSEVSL
                     SEFGRRAILALRQNLTSRIMELERNNLYSKYKDRIGEIITGEVYQVWKKEILVLDDEG
                     NELILPKSEQIPSDFYRKGDSIRAVVYKVEMRNNTPQIILSRTANAFLERLFELEVPE
                     IFDGLITIKKIVRIPGERAKVAVESYDERVDPVGACVGMKGSRIHGIVRELRNESIDV
                     INYTTNTQLFIQRALSPAKITSIKIDEENKRAEVYLKPSEVSLAIGKGGYNIKLASQL
                     TGYEIDVYRESAAEDEEDVSIDEFVDEIDEWIIDVLKGIGCDTAKSVLEIPFDELVKR
                     TDLEEETVKEIIRILKSEFE"
     gene            22935..>25114
                     /gene="infB"
                     /locus_tag="PLW22_04505"
     CDS             22935..>25114
                     /gene="infB"
                     /locus_tag="PLW22_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU73999.1"
                     /translation="MTSDNKIRLSKLAKEFNVGVGTLVEFLHKRGYDVENNPNAKVDQ
                     DVVTILEKEFGSELDLKKKTEQQKENLKALRDKKKTISIDDIEKDEDDFSDEPSDDEI
                     IIKTNTIETVTPTIEVEKPKIDLKVKGKIDLDTLGKKPKEPKPEPKPEEKPTPVKEQP
                     STPKEEKEAKPKQEEVIKPNVKVEAPKVVGKIDIEKAGKTHTKEAHRPHQKDEKPQPT
                     KEVIKKPAVEAEKEIPAEPEVTARTQEAEPEVYRAEVEKLSGPTIKGKIDLSRFEVKK
                     KPVASSSDFDKEGKKKKRKRIKKGDKVGIEQPQEVKVDREANKDDLKAKKLKKKNKKK
                     IIRPEVDEEEVQRQIKETLARLTSKGKTKSSKYRREKREILSARQREELERQEQEKQV
                     LKVSEFVSANELSAMMNVPVTDVIDVCMNLGLMVSINQRLDAETMALVADEFGYKVEF
                     VSVDVQEVIKEEEEDKPEDLKPRPPIVTVMGHVDHGKTSLLDYIRHTNVIAGEAGGIT
                     QHIGAYSVELEDGRKITFLDTPGHEAFTAMRARGAKLTDVAIIVVAADDAVMPQTVEA
                     INHASAAGVPIVFAINKIDKPTADPERIKGELANMNYLVEDWGGKYQCQEISAKQGLN
                     VDKLLEKVLLEAELKDLKANPNRRASGTVIESALDKGRGYVATVLVQNGTLHQGDIML
                     AGIYYGHVKAMFNERGQRIQEAGPATPVQVIGLNGAPQAGDYFN"
BASE COUNT         7016 a         4998 c         5540 g         7560 t
ORIGIN      
        1 aagcccaccg ccacgttcca cccccgaccc attgccgggt caggctttta tgccactgcc
       61 tgcttcatcc gttccattcc ctctcaggga aaagggttgg ggtgaggtcg tatcccacga
      121 ataacgaaca acttatacga atagtttcat ttgtcatcct gagtgcagcg aaggatcttt
      181 tgaaaagagt gcagtgtaca gtgtgcagtt taaagggaaa attgagagta gtgcttgttt
      241 tttttttcac tatcaactat caaccatcaa ctgtcaactc tgcttgtcat tccgagctag
      301 tcgaggaatc acattaaaaa gtgatatagg tacaaagaga tgcttgggca gcgcacagca
      361 tgccaaagaa ttgtctatcc tggtatattt gggcaaatat agctacagct catttacgta
      421 atcaataaac tcatacgtat ttccaaccca gttgagatac ctgaaaaaat cgatggttgg
      481 aattaccagc gatgcagtat ttaggtttgg gtgaaagcta agtcgttgag tgttttttag
      541 ttcactgtcg ataaacagat gcacatggcg agaggtgtcg tgaagtaatc caaaaggcga
      601 taccgaacct ggcgttaacc ccaggtagcg tttcatacgc tcggacgagg caaatgttag
      661 cttgccttgt tttagtagtt tttcgagttg atgaatgttc agcatcttac ggtattccag
      721 aattaccagg tagtgcctgt ttcctttgtg gttcctgaaa aagagatttt tgcagtgcga
      781 tgaatttatc ccttcccagt atttagaggc aatttcaaca gtgggagcct caggatgctc
      841 gtagtattca aatggaatac ttaattcgtt tagcgtttgg taaaccaagg ggtcgccacg
      901 gtactccata tggctaaagt actagatttt taaagttaag gtttacctgt tcgcaattga
      961 actcgttagg gttgtaattt tctccaagcc agtcggcaat tagagggtat tgtctatcgc
     1021 ccttattatg ttttgcaagg agttcaagta cttcacagta attaattata cccccagaat
     1081 cctcgggtgg cgatttgccc tgcccttgaa ggcaaacggg ttttttgctt agctcacctg
     1141 aatttgtagc aagaagcgaa attgatattg tccaaaaatc ggccagatca taaacataga
     1201 taaggctctc ccatttgttc aatgcattac ccaataaaat ttcttcctca ttcttgagta
     1261 ttgacttatc ggcattgtaa aaatgtgagt gcatattact ccactcaaag gctgcttgta
     1321 ttacctcatg aaatagttgt aggttgatat tttcatcaac cctaataatt ctgaatatct
     1381 cgggctttga gtttactatg cttaacttta cgtcaatcat tttgaggagt ttggcttatt
     1441 gtcaccaaat ataaacattt tttgcaagcg taaggattac tatggaagat ttaagttata
     1501 aacaaagcgt tttttgggtt aagcatatca taggtttaat ttccagttag aatgaaataa
     1561 attagcgaaa tataaaaaat aacccaaaag gtctttcttt ttgataaagt ttaataattt
     1621 tggataaaac gagaacgctt taatagtaat atatgattag attcctaaag gatattaatt
     1681 tgaggttaaa attattgtta gtattgatag ttttaacttt tttatatggt ttctatattg
     1741 atttttttga agggcctaag cctatacatg agtggcgcaa agcagattgt ttgtcaatta
     1801 cctataatta catgaagggt gagccttttt ttgaaccgaa aacaaatctt attttagaaa
     1861 acggtaacag gaatgcggtg tctgaatttc caattatata ttttttgatg gggaaactat
     1921 ggtctgtaat agggcaaaat gagtttttgt atcgtttatt aagttttgtt cttcttttgt
     1981 taggaattat tttatttgcg acagtagtac agtattatct aaaatcacaa aaatatacaa
     2041 ttatatatag cgtttttatg ttttcatcgc caattcttat tggatatgcc cacaatttta
     2101 tccctaatcc aatttcattt gctttaaatt tgattgctgg cttttttgta tttcaatttt
     2161 tacaaaataa taaattatat ggattaatag gcttctttgt ttttagtact ttagcaatac
     2221 ttatcaagat aacaagcata ttggttttta tgtcctttat tggagcattt attttttatg
     2281 aactttttga taataactac ttttggagaa aatataaaat aaaaatatca ttactttcag
     2341 gctcattcat tcttacttta gcattagtag ctgcatggta ttattatgca atttattata
     2401 atactaagta tgattctcac ttattttcaa caacaattcg tccaatatgg gaaatacctg
     2461 ctgaaaagcg aaatgaaatt ttttggcata taataaatac aatgataaat tatgcgtatc
     2521 acagagttat tgtttttgcg cttataattt ttggtgttgt tgcactattt tctaggtcaa
     2581 ttaacgcttt tcttaaatgg ttaatagtta tgtctatagg tgccttattt tcatacctca
     2641 ttctttggta ttgggttttt gatgttcatg actactattt aatagagata ctttttgtat
     2701 ttaatgtttt gttttttatc tttctttata aaattaattc gttttttagt ttcctagatt
     2761 taaaaaagat taatctagca ttatattttt ttataatttt tgtagttctc catgcataca
     2821 catgcagttg ggttcgcttt tcagcaaatc caaaattgat tgtaaataat atttttctta
     2881 aaaaaagaga tattgataca tggcgctggt ttaattatga ctatgggatt aatttaaaaa
     2941 cccttcaaag caaatctgat gagataaaaa aaattattca accatctgat accattttat
     3001 gcctacgtga ttattctcct aatgtacatt tatataccct tggaagaatt ggatattcaa
     3061 aatttctata taatggattt tccgataggg aagctattgt tgatgcatat aaaaggggtg
     3121 cacgatatgt tgtgttttta ggtagtgagg aggagcgggc taaaatacct tattcagatt
     3181 atgttgccta ccaatacgaa agaatttata tttttgatct taaaccctat aagaaaatac
     3241 aatgaaaata agtgtagtac tgccttgtta taacgaggaa ggtaatataa aattactata
     3301 tagtgaattg aggagtgttc ttacagataa gtatgagtat gaaatcattt ttgttgatga
     3361 tgggagtgtg gatggcactc tctcaacaat taaagaaatt gcaatggatg atagaaaggt
     3421 aaaaggtata tctctttcaa gaaactttgg gcatcaatct gcaattattg ctgggatgaa
     3481 tagtgcttcg ggcgactata taattactat ggatgcagat atgcagcacc cgccaacttt
     3541 aatcccaaca ttaattaaca aagcaaatga aggttatgat attgtactta caattagaaa
     3601 aagtactgtt gatgcaaaat ttattaaaag aataacatcg catttattct atcgtttttt
     3661 taattatctc tctgatattc cattaaaaga agggagtgcc gattttcgct tgatgacacg
     3721 aaatgccgct aatgttttct taggtttgcc tgaaaaagta cgttttaatc ggggtttaat
     3781 caattggatg ggttttaaaa cagcttttgt tgagtatcat tctcccaggc gcatttctgg
     3841 aaaatctaaa tatacattta ttcggatgat aaagtttgca attgctggta tactttcttt
     3901 ctcatctaaa cctttaaaaa ttccctttat attagggctt ttatcattca tttttggatt
     3961 gggttactca atatatgttt tagttcaatt tattaatggc atgactatcc caggatgggc
     4021 ttctactata attcttatac tcatgctagg tggcgctcag atgttaagtc ttggaatagt
     4081 tgggctttac atagcaagaa tatttgaaga aaccaagcaa cgtcctattt atatcattaa
     4141 agagcgaatt aacattgaat aattaaaaaa caaaaaggct atccgaaaaa ggatagcctt
     4201 taagtgggag taaagctgac ctaaccttta gtataccaaa agctctgctc cgttactaat
     4261 cacacaggta ataatttgag gaattttacc atcgggtgtt ttgtagtttt cttttacaca
     4321 ttttgtaaac tcatcaatat gggcgttttt aaccacattt attgtgcaac ccccaaaacc
     4381 cccgcccatc attcgtgagc caaccacata atcgagtttt tcagttaact caacgagctt
     4441 gtcaagctct ggacagctaa cctcatacat gtcacggagt ccgtaatgcg actggtacat
     4501 taactttccg aacgattcaa agtccttttg ggttagtgct ttaccagcat taaccacgcg
     4561 ctgattttcc tcaaccacat acaaacacct ttcccacacc ttactgctaa actctctttt
     4621 atggttgttt aaatcattga ttgttacatc gcgtagcgat tgtattgaat tattgtactt
     4681 tttaaggata tcaactccgg cttggcactc ctgcctgcgt ttattgtact ccgacgaagc
     4741 tagcgagtgc tttaccccgg tattaaccaa aatgatggaa tggttatcca tattgagttc
     4801 aaagggctca aactcaaggc tacggcagtc gagcagcagt acatggtttt gcttgccatg
     4861 cagcgatgca aactgatcca ttataccgca ttttaccccg gcatactcat gctcagccct
     4921 ttgggctatt tgggcaagtt gcatgcgcgt aaaaccaagc ccatagatat ggttcagcgc
     4981 aaaggccaat ccgctctcaa ttgctgccga cgatgacatt cccgctccaa ttggaacatc
     5041 gccaccaaaa acacaatcaa aacctgggat atctttaccc tcggctacca tttgggcaat
     5101 tacccctaat aggtagtttg tccagtgtgc atttgatggc tcaatcttgt aaacgggggt
     5161 tgtgaagtcg cgcctaaaat cggcagcgtg gaaccggtat aagccaagtc cgtttggttt
     5221 gatggcaaag tatgcgtaac ggtttatggc cgcaggcatc acaaaaccat tattgtagtc
     5281 ggtgtgttcg cctatcagat tcaccctgcc gggagctcta aaaacgaagg gatcggagcc
     5341 aaaacgatcc ttaaaagttg aaacaatcct ttctaccatt ggtttactct agaattatta
     5401 ccaatccact acggggtttt accttaaagt tttgtctatc gtttttacca atacgttttg
     5461 agcttagtac taattcgtca tcggaattac cttcagcaat aattgtcatt ttatactccc
     5521 tgtcatcgag gaggttgagg tttaagttaa agttaaagtt aaagtcatgc tcttggtttg
     5581 cagtcataca tccaatatac cacttatcac catgtttacg ggcaactgca atgctttcgc
     5641 caactttgcc ttcaagggca atggttttat cccatgttac gggaactcct gataaaaagt
     5701 tctgggcaat gctatctttt tggtagagcg ttggcgagtc ggctagcatt tgaaccccgc
     5761 cgtggtaaac tatatacatt gccatttgct gggccctggt agtcatggag tgggggttct
     5821 cccatgattt tacgaattgt tcaggggtta catgtcgcat tgagccggga gtgtaatcca
     5881 tggggccaac cacattcctt acgaagggaa tggtgaggtt atgaaccggt gtgcacttgt
     5941 cggaaaattt attgtactca agcccaagca ctccctcaat attaacaatg tttgggaaag
     6001 tgcgatttat tccggtaggc ttgtacatgc cgtgtaggtt tagtaaaagg ttgcgcttgg
     6061 cacattcatc agctaactta ttcacaaaat caactatacg ctggtcagcg cggtcgaaaa
     6121 agtctacctt gattcctgct actcccaatt tgctaaacat gtcgaggtta gctgtaaagt
     6181 ctttatcaat agtgttccac attgcccaaa taaatacctt aacacccttg ctgttggcat
     6241 agtcaatgac ctccttgagg ttcaggtttg gattaacctt tttaaaatcc caaagcttag
     6301 cccatccgtc gtcaatattg atgtactcaa tgccgttatt ggcggcaaag tcaaccatat
     6361 acttataggt ctgggtattt atccccggtt taaattcaac gccttgtagg ttccagttat
     6421 ggtaccagtc ccaaaccacc ttacctggtt ttacccaacc atagtccatc ttgggctcat
     6481 cggcaagcag gtaaaccaag ctattttgta gaatttcggc atcgttttgt gccaccatta
     6541 ctacacgcca ggggtatgaa cgattaccat tacttttggc aatatagttt tcggttttct
     6601 ttaccaccat ccttgaaatt tgaggccact tgaccagatg aagtctgcct ggcaattcct
     6661 gtttttcctt cttaggataa agagggaata ccgcttttac tgagttacct gcatccttgc
     6721 ggatgaacat gcccggatat tcgaagagat tcgattccga tatcacaacc agttcgccgc
     6781 ttggtgtttt atacatgaag ggggttaaac ctagagtgcc atcggctatg gtttgcatcg
     6841 attttggtaa gtaattaacc tcaaatggag taaggaaacc ctttacctcg gggaagtaaa
     6901 cagttgcact gttgggaatg ttgaattcac ttgtttcgtt ataaactata acctccctgt
     6961 caaatgctga aacaacacgc caagcaaaac cctcgttata gcaacggaac tcaaccgaaa
     7021 ggccattact ataattcagc tttagctggt tgaaattttc atcaatctcc tttgtagttc
     7081 cccaaagcaa tttcacagct tggtttttgc tattgaatgt tgcggtatgc ttccccaaaa
     7141 aatcgctctc tgcaaaagca ctcgttctca ttgctatacc cgaggcttca acaattgcat
     7201 tgccattaca ggatagcgag taggttaacc tatcctttaa atcgatattt gccactaaaa
     7261 tgccattggg cgattttaag gtgtaacttt gcccaaacag caaatctgta gtaaatatca
     7321 gagctgttag tatcgatatt cttttcattg tgttgccatt atgcatttta actagttttt
     7381 ggtaaccggt ttgaggctta cctgaaactc aaacttcttg ccctttaaaa catattcttg
     7441 caggtagttg tagaagaaac tctcgctacc aagtgcattt tgcgccaggt cgatataaag
     7501 atggttgacg ggactttcaa cgaggtcggg tgtatgtgtt gccttggtga gtgtttctaa
     7561 cgagtaagga tgtatgctaa agcagaacga ttttccggtt acctcaatcc cgcttgtacc
     7621 gttggggtta aatatgctta ctctgtccac atcgtagcgg ttaccgtttt cctgaggctt
     7681 tatgtatggt gtaaccatac tatcaacggt tttgcggtat aatccccaat gtgcagcgta
     7741 gctgcggtcg cggtagtttt cctgaggccc gcggccaagc cactcaacct gcgagaactc
     7801 tttaggcaac tcaagcataa ggccaacacg gggcaggttg aattttttgc tacaggtcaa
     7861 tttatagttg atatctacaa ctccgtcagg gttaataaca tgggtaatca cagcattaaa
     7921 tctcgattta ttaccttcaa gggtctggtg ggttagcact acaactccct tgccggttgt
     7981 ggaaattgta accttcgatt tcttaataat caaattgtct aacccatact tttgccaaag
     8041 ttttccgtgg aacgagaaac gagggtcggg gttgaatccc gtgccaatat cgttacttgt
     8101 aggggcgcgc caaaggttaa cccttggccc gttctgggct accatttcgc cattcgattt
     8161 ccattttgaa attgttccgg tagttttatt gaattcaatg ctaaaggcct ggccactaac
     8221 aatccacgta tctgcatttt cggttacatt caaattaccg ctagtggcag caatggtggt
     8281 gggtttttgt accagccagg gcatttcgta ctggtatgtt gccacctcaa agcccttttt
     8341 agcccatggc tcatcgtttg ccagttgaac cgataggtta agatggtaac gtttgcctgg
     8401 attgatttga gggagcgtgt atggaattgt taccgagccc tctccatttg gcttaacgtc
     8461 gggtaacggc agttcccctt tttgaacggt aatgccgtct tcagaaatgc tccatgttag
     8521 gttaaagctt tttaggttca ggaaggcata tgtattctta aggttaacgg ttttgcgatc
     8581 aaaatcaatc cctgtaaatt taatgtactg ttgtaccttt tttatttcgg ccatagcagg
     8641 tttaagggaa cggtcgggaa aagtaacgcc gttcaggcaa aagtccttat cgttgggttt
     8701 gtcgccaaaa tcgcctccgt aggcgtagaa tggtttgccg ttagatgtat gtttaatcag
     8761 accttggtca gtccagtccc atatatagcc cccaaccatt cgtgggtggc tctcgaataa
     8821 gtcccagtac tccttgaaat ggccattgct gttgcccatg gcgtgcgagt actcacatat
     8881 aagtataggg cgttcctttt tgtcgagttt agccatctcc ttcagcctat ccagggtagg
     8941 gtacatacca gcattaaagt cgtactctgt aagggctttt gaccattgta aggccgataa
     9001 cattcgcctt aacttttcaa aggcacccac accatcgaag ctaagggggc gtttaagagc
     9061 tttgctttca taatgaatag gcctttttgc agcatcaatt tttctgatgg tatcggccat
     9121 ggcaaccatg tttggtccgt cgcccgactc gttacctagc gaccatatta ttaccgatgg
     9181 atggttgcgg tcacgcatta ccatgctaac cccccggtca acaatagcat ccttccattc
     9241 ggggtaaagc actggcgatt gattacggta ctgccatagg tagtgcgatt caacattggc
     9301 ctcgtccatt acgtagagcc catacctgtc gcatagctca taaaaatatg gatgatgcgg
     9361 atagtgggct gtacgaactg cattgaagtt gttctgtttc atcaggacaa catccttctc
     9421 catgagctca tacgaaaccg tacgagcgtt gtaggggtcg aactcatgtc ggtttactcc
     9481 ctttattttt attggttgtc cgttaactag tagctgtccg ttttctactt taacatccct
     9541 gaacccaacc agctgctggt agtatctacc ctcaatctca acaataagtt gataaagatt
     9601 aggcgtctcg gcactccaaa gctgtgggtt gttcactatc tcatctactt taaaggtagc
     9661 tatcccactg ctcagtttgg gttgaaccgt tttttcaaac actgcaatgc cattactatc
     9721 aaaaagtgaa aggctgatgt tgggtagctg gctcgttgta tttgttagta ccccgctaac
     9781 tgaaagggtg gcctcagtat agcttttact gaatgaagtg ttaacctcaa tatcccagag
     9841 tgcattttga ggctgagcgt aaacaaatac atcgcgaaaa attccgccta atcgccagaa
     9901 atcctgatct tccaggtaac ttccatcgga ccagttgatt acccttgctg ccaaaagatt
     9961 ctgtccagcg gtaatgtatg gtgtaatgtc gaactcggcg ggggtcatgc ttccttgtga
    10021 gtagccaacg taatggccat ttatccaaag gtcaattgcc gattgtaccc cagcaaagtg
    10081 aattattacc ctactgctat tccaattctc agggatccgg aaggtgtggc ggtaaagccc
    10141 ggtctcgttt ccctcgtttg gaacaaaggg tggattagct gggaatgggt gtatttggtt
    10201 tgagtaaatt ggagtaccat accccttagt ttgccagttt gatggaacag gaatggtgct
    10261 ccattcgcta tcggtaaatt ctacatttga aaagttttcg ggagtgttca acgggttgtt
    10321 tatcagcata aatttccaat ccccatttaa ggattgcaca aattcagaat tgaaaatgta
    10381 ggaaatactg cttgactttt caggaaatgg gaccagtaga gtgtgtgagg gcagcctgtt
    10441 tatggcaaaa acctttgggt cctgccagtt ctgcgcttgc agcatagtgc tgcaaacgca
    10501 gagtaatatg aaaagtgctt ttcgcatcat catttagcaa ttttttcttg aatctagaac
    10561 agataaccaa ccaaaacgta caatcccgat gaaccgtaat ttttgtccat ggccacgcca
    10621 taatcggccg ataccacaaa gttttcattc attacgattt tcaaaccagc gccaaatgtg
    10681 gtatataaat ctttatagct gtttgaaaac agctggttgc gttgggcaac aggaacattt
    10741 gttaggtcca tatcgtaacc tttcaatacc ttgccagcat cggcaaaaag gttaaagcct
    10801 aggtaaaagt tttgcccaat ggcatggaag cggtatgcct tccaacgaag ctcaaaatta
    10861 ccgatagctg tatgctgacc aacaaccctg ttcatcaaaa cacccctaac ggtttgagcg
    10921 ccaccaaaac cttcgtagta gttgctggtg gttagatgct gacgggcgta aaaaggcacg
    10981 ttacctaggt tcccttcgta ccagagtcta tatgcaaacg atagtttttc ctttacaagc
    11041 gttaggtatt ggcgatggat taacgatact cgtccgtagt tatcgttgtt gccaatgaac
    11101 ttgggcgatg ccctaaaaag tagttctgtc catacccctt tcattgggtt agttaagcgg
    11161 tcgcgggtat caaaaaccag cccaaactta acactattat caaatccacc atctttttcg
    11221 tcggccgaga tgattcccca gctaacaagc tgatcgtata gaatgggaac atcggggata
    11281 agctcatcgt cgtcgcgacc tttgtttagg tggtcaatat ctactgttgc aacatcgtac
    11341 cagccaaggt caaaacctgc aatccactta aatttgctgt cggctattct gtcttcaagt
    11401 aggaaatcgg cttttgcaag cttacggtcg tatttgtaaa atacttgaga aataaaatcg
    11461 tcggtggcat tgatattttc aaaatcggcg ttgtaaacgg tttgtgtgcc gttaaaaccg
    11521 tagaatgggt atgtcctgtt ccggttaaag cttaggcgac caataaagtg tttgtttgga
    11581 ataagtgtgt aggaatcgaa gtaggcaatg gcatccatgc taccctttgt ataggccgat
    11641 agctgaaggt aaaggttgtg gttgaagtcg ggatacctgg agccgtcgcc aaaatcgaac
    11701 gagttaacaa tggctccata aaggaagccc aggtttgagt cgtagcctaa tgccggtaaa
    11761 acaccaactg tccagccggt ttttacttct tcgttggtct tcttttcctg attttcctgt
    11821 gcgaatgatg gcattgcaac catgcaaatc agtaatgcaa aaataacttt tctcatagct
    11881 ttaaattttg atttttggtt aaggttttgg atagctattt agaaaagtga tgtaacctgg
    11941 tcgttcaaat aaaaagtttg aacaatttta tcaaaagtta cacgttctaa ctaattgata
    12001 tacagatttt ataacacagg atgattgagg agattcttct aagtattttt tttagttgaa
    12061 ctcctaaagg cctttttata ttcggatggt gtcattccgg tttccttttt aaaaagggta
    12121 ttgaacgatg ttttggagtt aaaccctgaa tcgtaggcaa tacccaggag ggtctcgttg
    12181 tcgaagttag gatttgtaag cctttgcttg gcctcttcaa tcctgaaacc gttgatatag
    12241 ttgaaaaagt ttacgccaag ttccttgtta aggtaactgg agatttcctg ggaacttact
    12301 ttgagctggt cggctaaatc ctgaatggtt aactcattat tcaggtaggg cttgttctta
    12361 accatgtgct cataaagtaa atcgctcaat ttttgtgatg ttttggtctg taaatcttca
    12421 gttacctcta tttgtttagg tttttggtat tggtagagta ccggctggtg gatcccgtaa
    12481 tagcttatgg aaaaggtgaa aaccagtaaa cccaaatgga aaagctcaat gggattaaaa
    12541 gggaaaatat tttgggttga aaaaattata aaggtaatgc ccgaagcaaa aaagccagag
    12601 aggatccaaa tggctatatt tttgacccag tttagcatga tagtttccga gcggtacgag
    12661 aaatggtcgc ccagtgtgcg attatgtttt cggattagaa tgagcgatgc tcgccagtag
    12721 taaagaataa ctatcaggaa aagagcatag ttgaaaattg ccagccaagt gattggccct
    12781 ttgctgtaaa agttaacgga gtcaatatca attggtttgc cccaaacggc tgcaataagt
    12841 atgaaaaggt aaaacggaat gaggtgccta tgaatattcc ggagggttgt aggctttttt
    12901 gatgttaaaa gtttagtata gaagaacagg aaaggcccat agcttagcgg gataataatt
    12961 aaatcgggca gccaggttag atattttgca ttaagtaggg aataaagcat ctcgatacca
    13021 atcacaatta gccaaataac cagtaaccgg tcggctgctc ccttgtttgg ttttcggtaa
    13081 accagtaagg ctgcaaacca ggcctgaata aggccaatat ggtaaatggg tattacgttg
    13141 gtcatcgcaa taattttgca taaatgtaga aaaaagaaaa taaaaaaacc gctcagctga
    13201 gcggtttttt gttgtcctgc caggggtcga acctggactc ttctgatcca gagtcagacg
    13261 tgttgccaat tacaccacag gacaatgctg ttacaacgct gcaaagataa tgtttttaaa
    13321 aattgatttg caaaatattt ttgcaaattt ttatggtaag gctttttgtt aagggcaata
    13381 gccggctatt tgccggctaa ttttgcccaa gtgtctttca gagttacagt tctgttgaac
    13441 accagatgtt caggcgttga atcgtaatca acacagaagt aaccaaggcg ctcaaactgg
    13501 aacttatcta attgttttgc gtgttttagt gatggttcga gatacccctc aataactgtg
    13561 agcgattcgg gattgagtga cgatttccaa tcctgccctt cgggaacatc gtcggggtcg
    13621 ggtttggaga atagcctatc gaatagcctg atttcggctt ttacggcatg cggaatggaa
    13681 acccaatgta ttgtgccttg aaccttacgt ccatcgggcg agttaccgcc gcgtgaggct
    13741 gggtcgtagg tgcaatgtaa ctcaataaca ttgccttggg aatccttaat taccttctcg
    13801 cactttatga agtaggcata gcgcaagcgc acttcctgac cgggagccag acggaaaaac
    13861 ttcttgggtg ggttctccat gaagtcgtcg cgctcaatgt atatttcacg gctaaatggt
    13921 ataaggcgct taccagctgt ttcgtcctcg gggttattga cggcttcaag ctcttcaacc
    13981 tgtccttcgg ggtagttggt aataaccacc ttaagggggt tgagtacagc catgcggcgc
    14041 tcagcacgct tatttaaatc ctcgcggata caaaattcca gtaacgataa atcgattacg
    14101 ttatcgcgtt tggccacacc taccttatcg gcaaagtaac gtatggcttc tggggtataa
    14161 cccctgcgac gtaaaccgca aatggtaggc atacggggat cgtcccagcc cattactacc
    14221 ttttcctgta ccagctgtaa aagcttacgc ttgctcatta ctgtatagga gaggttcagg
    14281 cgggcaaact cgtactgttt tgaagggaaa atctccagct tctcaataaa ccaatcgtat
    14341 agggggcgat gcacatcgaa ctctagcgta caaatggagt gggtaatttg ttcaatggaa
    14401 tcggattggc catgtgcgta atcgtacatg gggtaaatgc accacttgtc gccagttcgg
    14461 tgatgctcag catgtataat gcggtacata atggggtcgc gtagtagcat attggggtgc
    14521 gccatgtcaa ttttagcgcg tagcaccttt tcgccatcct taaactcacc ggcgcgcatc
    14581 ctacggaaaa ggtcgaggtt ctcttcaacc gatcggttgc gccaaggact ttcctttccg
    14641 ggttgggtta cagtaccacg gtttatgcgt atctcttcct gcgactggtc atcaacgtag
    14701 gctaacccct ttttgattag cagctcggcc cactcgtata gctgctcaaa gtagtcggag
    14761 gcgtaaaact catttgccca ttcaaaacca agccaacgta tatcctcctt tatggagttt
    14821 acatactcta cgtcctcctt tacagggttg gtatcgtcga aacgcaggtt ggttttgcca
    14881 ccatactttt gagccaaacc aaagttaagg catatcgatt ttgcatgacc aatgtgcaga
    14941 tatccgttag gttcgggcgg gaaacgggta agcactcggc ctccatgttt gcctgttcga
    15001 atatcttcct caataatctc ctctaaaaaa ttttttggcc tttcgccgct gtcccctagg
    15061 gttaattcgt gttcggtcat tgcttttagt gttttatcag aaccaacaaa aatagaattt
    15121 tgttaacaaa tagatttttt aaagcacact ttttttaaag ggtatttcac tttccttcag
    15181 caaacccgct aaaggtattg atttataaca ggtttcatga aagttcacca aaaaagttga
    15241 atggaattgg aaagatccaa tttaacacct tggcaattat ggaaatgtgg aattactagt
    15301 aggtaacgaa gttgaatgga acctgagcca gttccgataa atcctcaccg gcctcacgaa
    15361 gttcggaatc atccttgtcg taataccagt tgatgtttaa cggtacctta gctttttgta
    15421 tctggttaag tatgatgata atgtcgtata ggaattttgc ggatgatgag ttaaagtaaa
    15481 ccagtttaaa ctgaaattca atgggttgga tttgttcttc ctttaacatc ttgctcttta
    15541 gctcgttgct aaacgattcc aaccaatcca aaatgggcat ataaaagtct ctaacgttct
    15601 cagggcgtga gtagcctgaa atggtcagtc tattctcgct tgggttaaaa tcgatatatg
    15661 gtgttgtgtt gctagcctgt aaaactaatg ggttcattct aatcaattta cgagttaggt
    15721 gtgataggaa taaagttgaa tgggaaatta atcatctctg aaatttcctc gcctgactca
    15781 aaaatttcgt cgtcatcctc atcgtagtac cagttaattt caacggcatt gcctttggag
    15841 tgaaacttca tgaaaaccat aagtatatcc ataaggaatt tggatgaggc tgagttgaag
    15901 taggtaagct tgagattcag tacaaggttg cttttgttaa agttagtgtt ttttgccaaa
    15961 acattatcct cgtagtcttc cagccatttg atgagtggcc tgtagaatcc aacggtattt
    16021 tcgggccttg aaaaacctga gatctcgaaa accgaatttt ccggatcgaa aaacacgcgg
    16081 ggtgtaaact cgctgggctc tatgtataat ggttccatgg tcactagttg ttttggtttt
    16141 gaagcgacac cttaattctc agagtaaagt atgtgtactg ttcatcaata tcgtcaaaag
    16201 taaactccag cttgttttca gtaaccttgg cgatattaat aatgcccaga ccagcaccac
    16261 ctttctgggt gacatttcca ctgaggataa cctccttgta aaactccttt aagccagctt
    16321 tatcgagttg attaactttt tccaaacgtt cttttaacgg tccaattttt acttttagta
    16381 atgaatttga gcaggttaag tctaccttgt ctttttccag tattagcgaa aatttaggtt
    16441 gatagttatt gctccccttt ataacatcct gatgcttata tatgttttcc aggctctcga
    16501 ccattgccga gtaaaccttt ttgcgaatgg taattccaaa gttgtaccgc tccagcaacg
    16561 atgtcatctt gttgagcaaa aaacctatct cctcatagga gagtggaccg taatgattta
    16621 ttaaaacttc gcctttttcc acgatagttt gaggtgtttg tataattaat tactatgata
    16681 tttaacgcaa agtttcacta attagccgac taaaggtaat acattttgac gaatagtata
    16741 tattgaattg atgaaaaaaa actgagtcgt ggtaaaaata agaaaataga ggatatacta
    16801 cgtttcattt taacccaggt accatggatt ttgaggaatg agtcggaatg aattggaatg
    16861 aatcggaatg ggtcggaatg agtcggaatg gtcagaatat gatggagtta gaggaagtta
    16921 gagggaaata gaaattagtg tttctattga caaatctggt ttaatataaa aaaagccgag
    16981 ttaatactcg gcttttttta tgtttgttag gtacactact caataatttt catttcgtca
    17041 atcagtcggg ttgcacctgc atacttatca attacaaata gcacgtagcg gatatcaacc
    17101 gatatggtgc gttgcagctt gttttcaaaa acaatgtcgc cgctcatagc ttcccagttt
    17161 ccatcgaaag caatgccaat aagttctcca ttgccgttca ttactgggct tcctgagtta
    17221 cctccagtga tatcattggt tgatataaat gccactggca ttttaccatc tttcccgtaa
    17281 cggccgtaat cctttgagtt atatagttcc ttgagtttgg caggaacaac aaattcccag
    17341 ttgtccggat cttccttctc cataactcca tcaagggtgg ttaggtagtc gtagtgaact
    17401 gcatccatgg ggtaatagtc ttttatttca ccataggtga ggcgcatggt gaaattggca
    17461 tcggggtaca tggctttgcc ttcgttcatt tcaagcaccc cagctatgta aaggcgctgt
    17521 ccgcgttgca acttttcgtt gaatggtgcc attactttgc taatttcttc gtatttgttt
    17581 gagatggaag ccgctgcaac gtacacaggg tctttttcaa gaacctttag gcttggcttg
    17641 tcaaggaaag cgttgaactt agctggatca gtaaacatgg ttttggcaaa catctggtca
    17701 acgaacttgt caacatcgcc cttgtacttt gttctgatgg tgttgaagat atcgggttga
    17761 taggttagcg gaacattttc catgaaaatg cggaacatgg ccttggccgt tttttggtcg
    17821 gttggtgtgt tgtagttctt gtagaagttc tcggagcgag cctttagcga cttactgatt
    17881 ttggtaacct cttcggtttt gttgcccttt agagcatcaa ccaatgaacg ggtcatggct
    17941 gccattccaa ttatttcagt tccgctgaat agtgcttcgc taatgtactg gctggcgtgt
    18001 aggtactcct tccggcctgt tacaccctca ttaatcaagc taagtgcttc gccatacttc
    18061 tgttgacgtt ctggcgaggc aagaacccac ttggtaaact cctcttcctg ctgtttcttg
    18121 aaatcgtaaa ccttaaggcg ctttagcgct ttgttcatgc caatggagtt tttccagtag
    18181 ttgctggatc cggcgtactt tgaggcgtac ttaatgttaa cggccttgtc ggcttgcata
    18241 tcctttagcc agatttcctg cttaatacct ctaacgtata tcctgttggc gttgagtata
    18301 ttaatacgtt cctgaacctc tacagaggtc atgtagcgtt gtgtgctgcc cgggtatccc
    18361 attatcatgg tgaaatcgcc attgtttacc cccttcagcg atattggtag gaaatgcttg
    18421 ggtttgtagg gaacgttttc gcgcgagtag tcggcaggtt tgttatcctt actggcataa
    18481 atgcggaata ccgagaaatc gccggtgtgg cggggccaca tccagttgtc ggtgtcgtga
    18541 ccaaacttac ctattgatga tgggggtgct ccaaccagtc gaacatcgtt gaatacctca
    18601 taaaccagaa ggaaatactg gtttccccca aagaaactgc gtacacttgc ggtgtagtgg
    18661 gtgccatcgg ttgcttgctt tgcaatttta cggccaatct ccatggcagc cttgtcgcga
    18721 tcagcttcgg acatttcagg tttaaaggca gcgttaacct ggtcggtaac atcctcaata
    18781 cgaataagga aagttgctgt taagccgggt gtaggtattt cctcctcaaa cgacttagcc
    18841 cagaaaccat cctcaaggta atcgtgttcc ggggtgctgt gctgttgaat ggcgccgtaa
    18901 ccacagtggt ggttagtgag aaccagaccc tggtcggaaa ccacctcgcc ggtgcatccg
    18961 cgcccaaaaa taataacagc atcctttata cttgcctggt tgaggttata gatatcgtcg
    19021 gcagtaagct taaagccttt ctgctgcatg gcttggatgt tatacttttg cagaagaggg
    19081 agaagccaca tcccctcgtc ggctttaatg gctgaactaa ccaggagcaa aaatcccagt
    19141 aaaagtgttg ttaatcgttt aatcatggca tttatttttt aggtttgttt ttttggacaa
    19201 ctaaatatgg tattagttta attcaacttc acacaatata gctacaatag cctaaaactt
    19261 agccggtggt aaggagtagt cccatgtttg gcaatactct ccctatgctt ttttgtagga
    19321 tagcccttgt tgatatccca gttataaagc gggtactcct tgctaatgtt caccatgaac
    19381 tcatcgcggt aagtttttgc aagtatcgat gctgcggcta tggaaagaaa tttagagtcg
    19441 cccttgataa tgcatgtatg gggaattccg ggataaggtt taaaacggtt accgtcaatc
    19501 agcaaatgtt ctggtttcag tttgctaagt tttccaatag cgatgtgcat tgcatgtatg
    19561 gctgcgttca gtatgtttat tttgtcaatc tcttggttcg atacacttgc tactgcccaa
    19621 tccaatgcgt agtgctcaat aaccttgcgg agctgcatgc gctgtttgtg gtttagctgc
    19681 ttggagtcgt taatgagcgg gtcgttgaag tcgggtggaa gaataacagc ggctgcaaaa
    19741 acagggcctg ccaggcatcc ccttccggct tcatcgcatc cggcttcaat cacattgaca
    19801 tttaggtact gcttaagcat tgctaaaaac atttttcgat gctactgtac aatacctgag
    19861 cagtattatc ccagctgaat tgttgttgtc ggttcagcgc gttggcgcta agtgtttctc
    19921 gcagttcagt atcgtaggct acacgctgca tggcatcagc catctcatcc acggaatagg
    19981 ggtcgaagta aatagctgaa ttacccgtaa cctcaggaat agccgtgacg tttgaagctg
    20041 ctatgggtat gccacatttc atggcctcca gcaagggaat cccaaagccc tcaaaggtcg
    20101 aagggtaaat tacagcatgt gcagccccag taattttagt gagttcatca aggcctttcc
    20161 tgccaaggaa tttgatatcg tttttgtggt tacagttttt ataggctatg ccgatatcgt
    20221 tggttttaaa catgggttca cctacaagca ggaggtttat tgctgaacca gtccttttgc
    20281 ggaatatgtc gtaggcttca attaaacggg caacattctt gcgcggatta aatgctccca
    20341 caaaaagaaa gtagggttta ccctcggaga tactctcccg tacccgctga atttcacttt
    20401 cggaaagagg cttgtaactg ttgcttgcac cgttgtatgc tacatcaatt ttatttgggt
    20461 cgatattaaa attattggca atatcggtac gtgaaaattg gcttacggta acaacccgct
    20521 ttgccttttg ggcaaaacgt gggaaaaagt agttcatgta gtgcgatgtg aagaaaggta
    20581 aaccctgggg cgcatggtaa aaattaatgt cgtgtataac tgctacctgg ggagtatcgg
    20641 atttcaacga aatgtagccg tcgggcgata ccagaacatc agcattaatg gctttgagtg
    20701 tttttggaat tttaaactca aaccagtagt accataagaa cggatgacgt gcagggggtg
    20761 gcactaccag tggtgttatg ttgttgctaa aaataaacga gttgtgatag ggcctgtcaa
    20821 atatgaaaaa aaactggtgt tccgggtggt tccgtgtaat ccgtatgagt gtttcatagg
    20881 taaaccagcc tatcccttcc agcttatcct taagcaaaag ccttgtgtta actgcaattc
    20941 tcaaagcaaa ataatttgag ctaattacaa cattttttta aatattagca acccaaaagc
    21001 gtgttttcag ctaccattat gatgacagct actggttttc aaacaaataa tttgcaattt
    21061 gttgaaaata ttgctatatt tgcaaaagtt ttgctggaag tggcagggga ctttgtcccc
    21121 tgttttgctt aaaaaatgcg taaggatgat tagtaaggaa gccatacttg aaatagtaac
    21181 ccctttgctg gaaaaggaaa accttttcct tgtcgatgtt tcggttggtg ccgataataa
    21241 gattattgta accgtagatg gcgacaaggg tgtttcaatt gactcctgtg ttagtattag
    21301 cagggggatt gaaaactccc tcgacaggga tagggaggat tttgagctgg aggtaacctc
    21361 agcaggatta ggccaaccct taaaaattcc tcgccagtac ataaaaaata ttggccagga
    21421 actggatgtt gtgcttaaaa cgggtgaaaa gataaagggt gtacttaaca gtgccgataa
    21481 caccggattt gtactcggtg taagcaaaaa ggtaaacatt ccgggttcta agaaaaaaca
    21541 ggaagtggta gagcccatcc agctgactta ccagcaagtt aaaagcgcga aagtagtact
    21601 taagtttaag tgatataata gggaaaaata tttaaagcaa tggagaatct gaacttaatt
    21661 gattcgtttc tggagtttaa ggaactcaag aatattgaca ggccaaccat gatgagtgtg
    21721 cttgaggatg ttttccgcag catgttggtt aagctttacg gttccgacga taactttgat
    21781 ataattatca acattgataa gggcgatttt gtgatttacc gtaaccgcga agtggttgag
    21841 gatggtgcgg tagaagaccc aaaccgtcag atttcacttt ccgacgcccg caagattgac
    21901 gaggattacg aggttggcga ggaggttact tcggaggttt cgctatcgga gtttggacgc
    21961 agggccattc tggcgcttcg ccaaaacctt acatcgcgaa tcatggagct ggagcgcaac
    22021 aacctttaca gtaagtacaa ggatagaatt ggcgaaataa ttaccggcga ggtttaccag
    22081 gtttggaaaa aggaaatcct agtacttgac gatgagggca acgagctaat tctacctaaa
    22141 agtgagcaga tcccttccga tttctaccgc aaaggcgata gcatccgtgc agtggtttac
    22201 aaggtagaga tgcgtaataa cacccctcaa atcatacttt caagaacggc taatgctttt
    22261 ctggaacgac tatttgagct tgaggtgccc gagattttcg atggcttaat caccattaaa
    22321 aagattgttc gtataccggg cgaaagggca aaggttgccg tagaatcgta cgatgagcgt
    22381 gtcgaccccg ttggtgcctg cgtgggtatg aagggtagcc gcattcacgg aattgttcgt
    22441 gagctaagga atgaaagcat cgatgtaata aactacacca caaacaccca gctgtttata
    22501 cagcgtgcct taagccctgc taaaattacg tccattaaga ttgatgagga aaacaaacgt
    22561 gccgaggttt acctaaaacc atcggaagta tcgctggcca ttggtaaggg tggatacaac
    22621 atcaagctgg caagccagct taccggttac gagattgatg tttaccgcga atcggctgct
    22681 gaggacgagg aagatgttag catcgatgaa tttgttgacg agattgacga gtggataatt
    22741 gatgttctga aaggtattgg ttgcgataca gccaagagcg tacttgagat tccattcgat
    22801 gagcttgtaa aacgtactga cctcgaggag gaaaccgtta aggaaatcat aagaattttg
    22861 aagtcagaat ttgaatagtt ttgccattag tttagttttg caaagtaata aataaatcag
    22921 gggaagaagc ctttatgaca agcgataaca aaatcaggct cagtaagctg gcaaaagagt
    22981 ttaacgttgg cgtgggaaca ctggtagagt ttctccacaa gcgtggttac gatgtggaaa
    23041 acaatcccaa cgctaaggtt gaccaggatg ttgtcaccat tcttgagaaa gagtttggca
    23101 gcgagctaga ccttaagaaa aaaactgagc aacaaaagga aaaccttaag gctttaaggg
    23161 ataaaaagaa aacaatatct atcgacgata ttgaaaagga cgaggatgat ttctccgatg
    23221 agccatcgga cgatgagata atcattaaaa caaacacaat tgaaacggtt accccaacca
    23281 ttgaggtcga aaaacctaaa atcgacctta aggtaaaggg taagattgac ctcgatactt
    23341 taggcaaaaa gcctaaagag cctaaacccg agcctaagcc tgaggaaaag cctacgccag
    23401 taaaggagca accttcgacc ccaaaagagg aaaaagaagc taagccaaag caggaagagg
    23461 ttattaaacc caatgttaag gttgaagccc ctaaggtggt tggcaaaatt gatatcgaaa
    23521 aggctggaaa aacccacaca aaggaggcgc atcgtcctca tcaaaaggac gaaaagccac
    23581 aacccaccaa ggaggtgatt aagaaacctg ccgttgaggc tgaaaaggaa atcccagctg
    23641 aacccgaggt tactgccaga acgcaggaag cagagcctga ggtttacagg gccgaggttg
    23701 aaaaactctc tggaccaacc atcaagggta aaattgacct cagccgcttt gaggtaaaga
    23761 aaaaacctgt tgcctcatcg tccgatttcg ataaggaagg aaagaagaag aaacggaaac
    23821 gtatcaaaaa gggcgataag gtaggcattg agcaaccaca ggaggtaaag gttgataggg
    23881 aagccaacaa ggacgacctt aaagccaaaa agctcaaaaa gaagaataaa aagaaaataa
    23941 ttagacctga ggttgacgag gaagaggttc agcgtcaaat taaggaaacc cttgcaaggt
    24001 taaccagcaa agggaagacc aaaagttcca agtatcgccg tgagaaacga gagatactta
    24061 gcgcccgtca gcgcgaggag cttgaacgcc aggagcagga aaagcaggtg ctcaaggttt
    24121 cagagtttgt ttcggccaat gagctatctg ccatgatgaa cgttcccgta accgatgtta
    24181 ttgatgtgtg catgaacctt ggtctgatgg tttccattaa ccaacgcctc gatgccgaga
    24241 ccatggctct ggttgccgat gaatttggct ataaggttga gtttgtgagc gttgatgttc
    24301 aggaggttat caaggaggag gaagaggaca aacctgaaga tttgaaacca cgcccaccta
    24361 ttgtaaccgt aatgggtcac gttgaccatg gtaaaacatc gctgctcgac tatataagac
    24421 ataccaacgt aattgcaggc gaggctggtg gtattaccca gcatattggt gcatacagcg
    24481 ttgagcttga ggatggacgt aagattacat tcctcgatac tcccggtcac gaggcattca
    24541 ccgccatgcg cgcccgtgga gcaaagctta ccgacgtggc tattattgtg gttgccgccg
    24601 acgatgcggt gatgccccaa accgttgagg caatcaacca cgccagtgct gccggtgttc
    24661 ccattgtttt tgccatcaac aagattgata agccaactgc cgaccccgag cgtattaaag
    24721 gggaactggc aaacatgaac tacctggttg aggactgggg aggtaaatac cagtgccagg
    24781 aaatttcggc aaaacaaggc ttaaatgtcg ataaactgct ggaaaaggtg ctgcttgaag
    24841 cggagcttaa ggacctgaag gctaacccta acaggcgtgc ttccggaacc gttattgagt
    24901 cagctctcga taagggtagg gggtatgttg ctaccgttct tgttcagaac ggaaccctac
    24961 accagggcga tatcatgctg gccggaatat actatggtca cgtaaaagct atgttcaatg
    25021 aacgcggtca gcgtatacag gaagcaggcc cagctactcc cgttcaggtg attggcttaa
    25081 acggcgcccc acaggctggc gattacttta acat
//
LOCUS       DAOOWJ010000029        24789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2024_length_24789_cov_7.754993, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000029 DAOOWJ010000000
VERSION     DAOOWJ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 24789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24789
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2024_length_24789_cov_7.754993"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1275)
                     /locus_tag="PLW22_04510"
     CDS             complement(10..1275)
                     /locus_tag="PLW22_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU74000.1"
                     /translation="MNSIRPFIILIKLARESMQFAYQSVKANKLRTFLSLFGITIGIF
                     AIISVFTMIDSLEKNVRDSLQALGKKVVYVQKWPWGGNEEYPWWKYLNRPEPTLSDLE
                     EIRRKSLLTDAACFNVAYNKQLKYNGNNIPDATIYGISTDFDKIRTFDLSEGRFFNQF
                     EINSGRPYAIIGSDIAKDLFQGANPLDKTIKIGGQKVTVIGVFAKEGKSAIGENSLDN
                     MVIIPLNLARTMVDLRNSGPWIMVKAKDDTSVEDLIDELRGILRAHHRLKPLDEDDFA
                     LNQIDLLKDSLDSIFASINFAGGFIAFFSIIVGGFGIANIMFVSVKERTSQIGVQKAL
                     GAKRYFILIQFLYEAVLLAVAGGVIGLLLIFVGTLAVTYGLDFNVTLTLPNIIRGLLI
                     SAIIGIVSGFVPAWMASRLSPVEAINTKA"
     gene            1587..3113
                     /gene="purH"
                     /locus_tag="PLW22_04515"
     CDS             1587..3113
                     /gene="purH"
                     /locus_tag="PLW22_04515"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOU74001.1"
                     /translation="MDGLKTIKTALISVYHKDNLDKIVKYLGEKNVTIYSTGGTMDFI
                     KGLGIPVVAVEELTGYPSILGGRVKTLHPKVFGGILARRENENDLFQLQEYSIPEIDL
                     VIVDLYPFEKTVESGASEPEVIEKIDIGGISLIRAAAKNFRDVLIVSNREQYERVYEM
                     LTEQDCATSLEQRRIFAAEAFATSSHYDTAIYDYFSPEFPQSFFRKSLKGVRGLRYGE
                     NPHQKGVFYGNFDEMFDQYCGKEISYNNLLDIDAAVNLIREFEEPTIAILKHNNACGV
                     ASRPSILQAWADALAGDPVSAFGGIIISNRSIDETTAKEINKIFFEVLIAPNFDNAAL
                     EVLQAKKNRIILRMKDFNLPQQVYRTALNGVLMQERDSVTESDSMLQLVTQKSATPNE
                     VNDLLFANKIVKHTKSNAIVLVKNLQLVGSGVGQTSRVDAVKQAIAKAQSFGFDLNGA
                     VMASDAFFPFPDSVQLAYEAGVRAVIQPGGSIKDQESINFCNANGIAMIFTGIRHFKH
                     "
     gene            3135..4154
                     /locus_tag="PLW22_04520"
     CDS             3135..4154
                     /locus_tag="PLW22_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU74002.1"
                     /translation="MGLFSFLTQELAIDLGTANTIILFNDKIVVDQPSIVAIDHSTGK
                     MIAIGEQARAMHGKTHENIRTIRPLRDGVIADFNAAEQMIRGMISMINTKNPIFTPSL
                     RMVIGIPSGSTEVEIRAVRDSAEHAGGRDVYLIYEPMATALGIGLDVMAPEGSMVVDI
                     GGGTTEIAVIALGGIVCNKSVRIAGDGFTADIQAYMRQQHNVKIGERTAEDIKINVGS
                     ALPDLDNPPADFIVRGPNLMTALPIEVPVSYQEIAYCLDKSLSKIESAIFNVLEQTPP
                     ELYADIVQKGIYLAGGGALLRGLDKRLTEKVNIPFHVAEDPLHAVARGTSIALKNLSF
                     PFLMR"
     gene            4174..4995
                     /gene="mreC"
                     /locus_tag="PLW22_04525"
     CDS             4174..4995
                     /gene="mreC"
                     /locus_tag="PLW22_04525"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOU74003.1"
                     /translation="MNNLYEILKRLYIHLIFLILLGISLSLYFNTTYYQKSQVGAVTK
                     SISGLWHKQASQVTGLFQLQRSNVLLLLENVRLRNEIEGYRLKLQMLQDSFMRIEDTS
                     GLFRYIPARVIDNSINSRHNYITLDAGSKDGAYKEMGVICKNGVVGIVSATTKHFASV
                     ISLLNTDVRVSAMHKKSGAFGAVSWDGMNYRRVVLDEIPLHVNVAVGDTIVTSGFSTI
                     FPRDIPIGRVVDFYNKDGSFLQITVELFADFKTLDYVYLVDSKVKSEVDRLQTMK"
     gene            4995..5513
                     /locus_tag="PLW22_04530"
     CDS             4995..5513
                     /locus_tag="PLW22_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74004.1"
                     /translation="MYSDILKYLGLTVILLLLQFLVLNNINFMGYVNPYIYILIILLL
                     PYRITRWMLLIIGFALGFAVDYFSNTLGLHTTASLVLAYFRPVILNRWSFKSVQDIRG
                     VPDVTNTSLEWFVVYALIAVLVHHFTLFVLEVFTFKHFWLTLVRIILSTAVSTFFIVI
                     IELLRAPNKMSR"
     gene            5516..7363
                     /gene="mrdA"
                     /locus_tag="PLW22_04535"
     CDS             5516..7363
                     /gene="mrdA"
                     /locus_tag="PLW22_04535"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU74005.1"
                     /translation="MLRAESSRRVVVSVIIMLVFAAIIGKLFYIQVIDSSYKFSAANN
                     VLRYITQYPARGLVLDRHGVPMVSNKPVFDILMVKRQVKPFDTLEFATMLGLTVEQVR
                     DAFIQATKQKGYSPRKAVVLLKQISAENFARFQEVMYKYQGFEIQPRTVRSYNPPIAA
                     HLLGYVGEVDDRDIKNNPYYQQGDYIGISGIERSYEHILRGKKGVKIFMVDVHNRIKG
                     SYEQGKFDTLAIPGKNITSTIDARLQEYGERLMQNKIGSIVAIEPKTGEVLAMISSPT
                     YDPNLLVGRQRTANFKVLQQDSLKPIFNRSIMALYPPGSTFKVVNALIGLQEGVVTPE
                     TRYSCYGGYTVGRGVGCHNHPSPTNLIQSIQVSCNTYYCHVFRNIIDKREFGSVENGF
                     SVWKRHVESFGYGNKLNIDLPHELKGIVPSVSFYDKYFRKGGWNSLTIISLAIGQGEL
                     SVTPLQMANLVATIANKGYYIAPHVVKSIEGDTLNSLYREKHITSIEPKYFDYIIEGM
                     DLAVNGAPGSGSTARIAALPDIRICGKTGTAQNPHGKDHSVFFAFAPKDDPKIAISVY
                     IENAGFGATWAAPIASLMIEKYLKDSISRPDLERYILDANLLDRRAKAK"
     gene            7347..8768
                     /gene="rodA"
                     /locus_tag="PLW22_04540"
     CDS             7347..8768
                     /gene="rodA"
                     /locus_tag="PLW22_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOU74006.1"
                     /translation="MQRRNNILRNLDWVATILYVVLVLMGWLNIYAAVYSDEHRNILD
                     FSQRYGKQLVWIFFAFLMAIVILYSNDRIYPAFAYIFYGIILITLISTLLFGRVVNAS
                     KSWIPIGSFGLQPAEFAKIATALALAKLMSSYGFKLSGFWSYVKVAVIIGLPVGIILL
                     QNDTGSALVFGALILVLYREGLPAWILSYLVFLVIVFILTLVYSTPVLITFIFVVAVT
                     LFVVFTRQHRDAFRISIGAAGIGLLLYISSELFHLRLSNALIFLIPVLISAVLGFLYA
                     YKNRLRQVYAISFFLIATIGFSMSADYVFERVLDVHHQKRINDLLGIEDDPLGWGYNV
                     NQSKIAIGSGGFWGKGYLSGTQTKFNFVPEQSTDFIFCTVGEEWGFLGSTVVVLLIAL
                     FLLRLLKIAERQTEHFARIYGYGVVSILFFHAAVNIGMTIGLVPVIGIPLPFFSYGGS
                     SLWAFTILVFILLRFDASRYGAY"
     gene            complement(8725..9351)
                     /locus_tag="PLW22_04545"
     CDS             complement(8725..9351)
                     /locus_tag="PLW22_04545"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOU74007.1"
                     /translation="MNFLAHLYLSGSNIDIRLGNFIGDHVKGKQILAYPQGVQTGIKL
                     HRTIDQVTDRHEAWKSCREKLRPSYGRWSGVATDVMFDYILAKEWSTYSNRPLKGFTR
                     TFYFQMLQRYHMLPPAVRGFLPFMIQSNRLYSYSTDEGVTRALEIMGNYTTMKGNPSL
                     ALQIVKENYIAFSAGLQELLYALIQVAEKEYGIPISMPHSVKHRTEGE"
     gene            complement(9363..10148)
                     /locus_tag="PLW22_04550"
     CDS             complement(9363..10148)
                     /locus_tag="PLW22_04550"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOU74008.1"
                     /translation="MSQGKKIYFASDLHLGLPNLEQSLPREKLFNTWLDTISHDAEEV
                     YLLGDIFDFWFEYRRVVPKGYTRFLGKIAQLTDSGIPVHFFTGNHDVWMFDYFEKELG
                     VQIHREPIVRELYGAKFLLAHGDGLGPGDPGYKLLKRIFTSPTLQWMFAKLHPNFSLW
                     FGNQWSVSSRYSKEITHVFRGEDEQITKFSRHKLQDEHFDFFIYGHWHSPIIYPLSEK
                     SNLVVLGDWLVSNTYSVWDGKELKLIRYQPPKADEVLATTTFL"
     gene            complement(10157..11311)
                     /locus_tag="PLW22_04555"
     CDS             complement(10157..11311)
                     /locus_tag="PLW22_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU74009.1"
                     /translation="MRFRYFVSIVLIVFLAGCSSKDAVVISVKVKSPEKTTLFLSKID
                     FNKTIKLDSVRISAGESVKKFKVKQGEEPEFYSLSTSKGLAITLLASKGEKINVILDL
                     NNAIDYQVSGSEDSEKVRWVTRTFAKSKTAVDELSKRYLNAESNSERELINNQFEQER
                     KRVKDELTKFVWENPMNKASIMALYQKYNDNLYIFDSSDDLLLIKMVATAWKALYPES
                     SYTKGMLEDIKNIENRIANAKMQQLIQNAEMPIPDLSIPDRNGRSIKLSSLKGKVILL
                     DFFTSENTASLLDNRELLEIYKKYKSRGFEVYQISFDATREGWINYIENAGLPWISVR
                     EDDPSASNAALFYNVRQLPANYLIGKDFNIIGKNLYGTNLQKALDSYLNK"
     gene            11432..12553
                     /locus_tag="PLW22_04560"
     CDS             11432..12553
                     /locus_tag="PLW22_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU74010.1"
                     /translation="MRAKPDGSYISYFSNLVKVNGGINLAQGIPGFQPPTELINILKD
                     IANSQHHQYAPGIGNLQLRALIENSYGVEPDELLVVQGATEGLSLVYTYLFRMLGKNL
                     VTLSFDPAYESYSRLPQIFNQKFVPFSLINEEFVDFDELKRCIEENKVKLIFISSPGN
                     PYGKVWTKAETCKLLELANELNFFVVFDSVYEELYFSQKPYIPLDNISEKLFIVSSFS
                     KSLCITGWRVGYVIHHPSHTKAIRAIHDYIGLCAPSVMQQALAVYLAQFQYGKAFMDE
                     LRQRVKSSFDSLSGMLNRLNFTVPKIDGGCFIWAKLPQPFTDGFEFASDLYDATGVAV
                     IPGEHFSSTKVNWLRFNVARPLEEIDLAGNRIADFMSKQ"
     gene            12550..13662
                     /locus_tag="PLW22_04565"
     CDS             12550..13662
                     /locus_tag="PLW22_04565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU74011.1"
                     /translation="MIIISTILIAIYTIAILWLLKGLLTNNWLESNGAPSQTVNGISV
                     IVAFRNERENLPKLIASLEAQDLPHEFWEAILVDDGSTDSGFDLVMSCKSQLPLKPLV
                     LPDGQAGKKQALLYGLSAASFDTVVFTDADCTHTPQWLSSIRESMGSTDFLQGEVKPQ
                     FEKGSIISMFEALDYLSLMAVSAASFAVGKPVIASSANLAFRRSKVNVDTKVLRTDIA
                     SGDDMFLLHHAKTITDLRFRFLASPRVAAYTSFSGGIKGFVLRRSRWASKATAYTDSL
                     TLLLSFLVFTLNFWIILLAILTVLGVITFNLPISLLAAKAIVDLPFLAYYLFKTKQQN
                     LLAVFIPLQILYPFYITFAAILGLIKGTTWKGRINS"
     gene            13638..14879
                     /locus_tag="PLW22_04570"
     CDS             13638..14879
                     /locus_tag="PLW22_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU74012.1"
                     /translation="MERKDKQLAELARSGSPMRIAIRRLLADRMAVASLLLIASIAII
                     AFLGYLIIPDSSPYANGQHLQLATLKPGSKVKFLRVAKQHEVKKRNILGRMFYGQEQD
                     YNEIPVDSIYFDGIRVYASEYNPIPEGDAIVAELTLPEILFRLNPEKKYLLENDTITA
                     FLYNGQHAIMAVADAQQRVLSQNLVTRRFLLGTDRFGRDLLSRLVIGARISLSVGFIA
                     VGIALIIGIVLGAIAGFARGIIDDAIMWMVNVVWSVPTLLMVIALTMVIGKGFWQIFV
                     AVGLTMWVDMARVVRGQVLSVREKEYVEAAKVLGYGPWRIIFRHVLPNIMAPVIIVSA
                     SNFSTAILLEAGLSFLGIGVQPPAPSWGTMIRDHYGYIIMDKAYLALIPGFAMMLLVL
                     AFNLLGNSLRDALDPREIEKA"
     gene            complement(14918..15265)
                     /locus_tag="PLW22_04575"
     CDS             complement(14918..15265)
                     /locus_tag="PLW22_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74013.1"
                     /translation="MKTKVIHIREYLPSIISTRKAIKEIRESISFDNNCKYIFDFSGI
                     FFISRSFADEFYKFVTYSHIDYSILNANKNIEEMLNAVSRISKKDIKTDTDEITFTYF
                     KNINELHSFLSSF"
     gene            complement(15360..16259)
                     /locus_tag="PLW22_04580"
     CDS             complement(15360..16259)
                     /locus_tag="PLW22_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU74014.1"
                     /translation="MQIEIIVKKPANYNLGASLEVLIDFLSQIQKWDKGSKLIINLQN
                     IDFTYPLTLLPIASYLKYLSSLYYDIDFVYNKNTQKYLNVIKFPYGLSYDYNELTIEL
                     KKYKNKTYLPICSIPSGSNNSKIRDQILSLFETILVSQTNIKGQLKIAVSYIINETID
                     NIVEHANVSNGWIMAQNYPKKGFLDVCIADTGDGILESYKKTKIFNISTDAQAINKAI
                     NGLSTKEQGNTRGYGLKTSRKMLVEGLGGMFLLFSGNSFYIWNTNIETIFSDIPYKWK
                     GTIVAVRIPHDIPSNFNYTNYLE"
     gene            complement(16383..18107)
                     /gene="lysS"
                     /locus_tag="PLW22_04585"
     CDS             complement(16383..18107)
                     /gene="lysS"
                     /locus_tag="PLW22_04585"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOU74015.1"
                     /translation="MALYEELSEQEIFRRQALDELRSLGIEPYPAAMYPVTHSTADIL
                     SDFENMSQNRAEVYIAGRIMARRIMGSASFFELQDEKGKIQVYVKRDDICPGEDKTMY
                     NTVFKKLLDIGDIVGVKGFVFRTNMGEESVHALELTLLAKSIRPLPIVKEKDGKVFDA
                     VTDPELRYRQRYVDLIVNPGVRDVFRKRATIINTMREFFNSKGYLEVETPILQPIPGG
                     AAARPFVTHHNALDIPLYLRIANELYLKRLIVGGFNGVYEFAKDFRNEGMDRTHNPEF
                     TVMEIYVAYKDYFWMMEFTEEMLERVAVAIHGTTKVTFGDKEIDFKRPFRRMTMSDAI
                     KQYAGVDITGKSEDELRAICKQMEIEIDDTMGKGKLIDAIFGEKCEEHLVQPTFIMDY
                     PIEMSPLCKRHRNNPELTERFELFVAGKELCNAYSELNDPIDQLERFQEQLRLSERGD
                     DEAMYIDMDFVRALEYGMPTCSGMGIGIDRLTMFMTNQPSIQDVLFFPQMRPEKKPQK
                     DTAEAYAQKGIPADWVPVLQKMGFNTVASLNGVAAGKLFNDLCGYNKKNKLGLPNPTM
                     DDVKRWVE"
     gene            18282..18908
                     /locus_tag="PLW22_04590"
     CDS             18282..18908
                     /locus_tag="PLW22_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU74016.1"
                     /translation="MAVKEQIVEGALELFKRMGIRGITMDMLAEHLGISKRTIYENFQ
                     NKDEIVEACVLLENESMQAMVQSILNKSEHYIETYIMFMWSHINKLRNVSPLFMYDLK
                     KNYPKTICKQAGEFDHYLKERMVEFIEKGKADNLFRAEVNPQIVASVVFEFTKLLRSG
                     FEPGSNSLNDFPLAEVFEHMAVNFIRGLATSKGIELVDYYYEKHKQIR"
     gene            18930..20258
                     /locus_tag="PLW22_04595"
     CDS             18930..20258
                     /locus_tag="PLW22_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU74017.1"
                     /translation="MRKLITIFSLAGFVLSTSLALGQQKPDTLHLTVKQAVDLAIQQN
                     LMHKVTELELQKKQEKVKEYMAALYPNIKASASYTRNLKLPVIFMPEGSPFGSTLTIG
                     SDNSYSASISASMPLFVYNVFESIRLGQKDVEISQEKLRESKINLAANIKTTYYNVLL
                     LRRTRDVINQSYQNAVANFNNIQKLNANGMVSDYDFIRIKVQVDNIYPNLVQTENAYT
                     NLLNIFKVLLNIDINTPVELDEKELTNYNFSEMALADSSWLSSNSTLRQLTLTRQMLS
                     IQEKMVKGANYPSLVAIGNYQYQTQANDYKFSNYNWVPTSMVGLQLNVPIFSGFSVRK
                     QLSQVRISQHQVDMQAQFTQNNLTVQVQNAVNAVNAAVKKVNSAKGNVDLAEKGYTIA
                     QTRYNTGQATLLELNDAENAMLQAKLNLIQAQLEYLVAKTDYEKIIGESF"
     gene            20294..21340
                     /locus_tag="PLW22_04600"
     CDS             20294..21340
                     /locus_tag="PLW22_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU74018.1"
                     /translation="MRTINTFKAITLASAVVLLASCQGNRADEKQLATTATEVKVVTQ
                     VVQPVSINRQFRYPATIQAYKENHLAPATAGRVEKVLVDVGDRVKAGDVVAMLDRTTY
                     QQAKIQFEKLKIDLQRVDSLLKVGAIPQQQYDAVKMQYDIAKTNLEFLDDNTSLKSPI
                     NGIITGKYINDGELFTMSPAPGVGKPAVVSIMQLDKLKLLVGVSSTYLNAVKPGMKVT
                     VKTELYPDKEFTGTVEKVYPTIDNMTKTFTIEVVVPNPDKMLRPGMFATAYINVGVGD
                     ALLVPAFVVLKQSGTNERYVFVYDNGKAIRRNIEQGDVFDDKVEIIKGLSAGEEIIVE
                     GQTIVFDGQPVVKK"
     gene            21359..24502
                     /locus_tag="PLW22_04605"
     CDS             21359..24502
                     /locus_tag="PLW22_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU74019.1"
                     /translation="MSLYSSAVKNPISTLMIFVGVIVMGIYSIFYIPIDLLPEMEIPS
                     ISVITFYPGASAEDIEVNVTKPIENQLNALQNLKKVTSSSKDNISFVTCEFEYGINLD
                     EASNDLRDAVSIAENSLPDGCEKPSIFKFSSSMIPVVIYGIQADRSYAALEKLIDEKI
                     IEKLSRIDGVGSVTIIGKPIRIIDVTVDPRKLDAYNITLEQVGAVIGAENISLPAGRI
                     EVGRMEFPVRTNGEFRNSDVVKSLVVANNGGKLVYMKDIATVKDTLKSVLVDERIDGS
                     LGVRLMVQKQSGANTVKIARLVRESMDELQKDLPSDVKVVKIIDSSEFIQNSINNLSE
                     TLIYAFVFITLVVILFLGRWRSTFIIMLTIPISLIGAFIYLFISGNSINIVSLSALSI
                     AIGMVVDDAIVVLENITTHIERGSSPMEAAVFATREVGLAVIATTLVIVAVFMPLTLI
                     GGLIGVFFRQLGWIVTITVSLSTLAALSLTPMLSSRILKGRSYYKHHRSRFLESVNRF
                     WGFFDTIYVKSLAWTLKHKALTLIIAGLVFIGSLGLAGVVGTEFLPSQDNGRINVNME
                     IPQGIRIDEAKALARDFEQKVKKKYPEVTLISTSIGADEGGSLKSVFQKSASYIINYS
                     IMLTSADDRTRSIFEIADSLRKDITGYTQVTSSYVDPGGNRNSRITLGMGGGSNLEVK
                     ILGYDINQTNYIAEQIAGTLEAEIGFRDISISRDKDRMELQVIPDREKLAQLGLNTSM
                     LASAVRNRIYGLTATKYREEGTEYDVILKYNDANLTSVSDIENITFKGPMGNMVRVGD
                     VAQIKQVYTTPNLEHEDKIRVVKVVSALSGIDLGTASSIINAKLATLTIPDNVIVEIG
                     GAAKDMADTFQDLMLLFVLIMVLTYLVMAAQFESLREPFIIMFSVPFALTGVFISLYV
                     TGTTLNMISGIAIVMLVGIAVKNSIVLVDFINLLVSRGRTISQAILEGGKSRLRPILM
                     TSFTTLLGMLPMALSKGEGSEIWRPLGISIVGGLVFSMLISLVIVPIIYALFANARIK
                     RERKKLQASDSEISFDD"
     gene            24521..>24789
                     /locus_tag="PLW22_04610"
     CDS             24521..>24789
                     /locus_tag="PLW22_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74020.1"
                     /translation="MKAIFIAYNQALSDKVDSALQIAGVKGFSKWADMLGAGSQKGEP
                     HLGTHTWPSMNSGMLTIVDDDKVDKLLSYINQINKVAEEQGIHAFV"
BASE COUNT         6873 a         5003 c         5398 g         7515 t
ORIGIN      
        1 tttgcttggc taagcctttg tatttattgc ttcaacagga cttagacgtg aagccatcca
       61 tgcaggcaca aatcccgata ctattccaat aatcgccgaa ataagtaacc cccggataat
      121 gtttggcagg gtaagcgtta cgttaaaatc caacccgtag gttactgcca gtgttccaac
      181 aaaaataagc aacaggccaa taacccctcc cgctacagcc aatagaaccg cctcgtagag
      241 gaactggatt agtatgaagt atcgttttgc ccctagtgct ttttgaacac caatttggct
      301 tgtgcgctcc ttaaccgaaa caaacataat attggctatt ccaaatcctc caacaattat
      361 ggagaaaaat gcaatgaatc ctccggcaaa attgattgat gcaaaaatgg agtcgaggct
      421 atccttcaac aggtcaattt ggttaagcgc aaagtcatcc tcatcaaggg gtttaagccg
      481 gtggtgggcg cggagtatgc cacgtaactc atcaattaaa tcctcaacgc tagtatcatc
      541 ttttgcttta accattatcc atgggccgga attccttagg tcgaccatgg ttcgagcaag
      601 gttaagcgga ataattacca tgttatcgag gctgttttcc ccaatggccg atttcccttc
      661 cttggcaaat actcctatca ctgtaacttt ttgcccgcca attttgatgg ttttatccaa
      721 tggatttgca ccttgaaaaa ggtctttggc aatatcgctg ccaattatag catatggtct
      781 tcctgagtta atctcaaact ggttaaagaa tcggccttcg cttaagtcga aagttcgaat
      841 tttatcaaaa tcggtgctaa tgccgtaaat ggtagcatcg gggatattgt tgccattata
      901 ctttagctgc ttattgtatg ctacattaaa gcatgcagca tcggttagta agcttttcct
      961 gcgtatctcc tcaaggtcac ttagggttgg ttctggtcta ttgaggtatt tccaccatgg
     1021 gtattcctca ttgcctcccc aaggccactt ttgaacgtat acaacctttt tccctagcgc
     1081 ctgtaggctg tcacgcacat tcttctctaa gctgtcaatc atggtaaata ccgatataat
     1141 ggcaaaaatg ccaatagtga tgccaaacag cgacagaaag gttctaagct tatttgcttt
     1201 tactgattgg taagcaaact gcatgctttc gcgagcaagt tttattagta ttataaacgg
     1261 acgaatagaa ttcattctct ataagtatta tgcttgagat ttttttcaga tattaccaaa
     1321 tgctcattca acaaatatag ccaaaggttt acttttcaat ggccttagat tgttaaatag
     1381 tgtaacattt tttagaatgg ctgacacacg gaaggtttaa tgcgattaag ttaaacttgg
     1441 caaaatgtca cattttatag tgtcaattat tttaaatgat gttgaaaaaa tttaattgct
     1501 aagtattggt ttttcaaagg atttaaccaa ttttaggggc aggttacagg attcatattt
     1561 tttacaagta attgaatact agcaagatgg atggcttgaa aactataaag actgcactaa
     1621 tatcggttta ccacaaggat aatctcgata aaattgtaaa gtatttaggc gaaaaaaatg
     1681 ttaccattta ctcaaccgga ggaacaatgg atttcatcaa ggggttgggg attccggttg
     1741 ttgctgtgga ggagctcacg ggctatccct caatacttgg aggcagggtg aaaacattgc
     1801 acccaaaggt ttttggtgga atacttgccc ggcgcgaaaa cgagaacgat ctttttcagc
     1861 tccaggagta tagcattccc gagattgatt tggttattgt tgacctttac ccttttgaaa
     1921 agactgttga gtcaggcgcg tcggaacctg aggtaattga aaagattgat attggcggaa
     1981 tctcacttat cagggcggct gctaagaatt tcagggatgt tttaattgta tcgaacaggg
     2041 agcaatacga aagggtatac gaaatgctca cggagcagga ttgtgccaca tcgctggagc
     2101 aacgtaggat attcgcagcc gaggcgtttg ccacatcttc gcattacgat acggccattt
     2161 acgactattt tagccctgag ttccctcaga gcttcttccg taaaagctta aagggtgtta
     2221 ggggattgag gtatggtgag aatccccatc agaaaggggt tttttacggc aatttcgatg
     2281 aaatgttcga tcagtattgc ggtaaagaaa tctcctacaa taacctattg gatatcgatg
     2341 cggcggttaa ccttatcagg gagtttgaag agccaaccat tgccatttta aaacataata
     2401 acgcctgtgg cgttgcttca cgccccagta tccttcaggc ttgggccgat gcacttgctg
     2461 gtgatcctgt atcggccttt ggtggcatta tcatatcgaa ccggtcaatt gatgagacta
     2521 cggctaagga gattaataaa atcttctttg aagttctcat tgcccccaac tttgataatg
     2581 ctgctctaga agtgctacaa gccaagaaga acaggataat tctgaggatg aaggatttta
     2641 acctgccgca acaggtttac cgaactgcct taaatggagt tttaatgcag gagcgcgatt
     2701 cggttaccga atccgattcg atgctgcaac tggtaaccca aaaatcggcc acgccaaacg
     2761 aggttaacga tttgcttttt gctaataaaa ttgtaaagca taccaaatca aacgccattg
     2821 ttttagttaa gaacctccag ctagtaggga gtggcgtggg gcaaacatca agggttgatg
     2881 ctgtaaagca agctattgca aaagcccaga gctttggatt tgacctgaat ggggctgtaa
     2941 tggcctccga tgcctttttc ccattcccgg atagcgttca gcttgcctac gaggcagggg
     3001 tgagggctgt tattcaacct ggcgggtcaa taaaggatca ggagagcatc aatttctgca
     3061 atgccaacgg cattgccatg atttttactg gaatacgaca ctttaaacat tagaaaaata
     3121 aatattaatt cgatatggga cttttttcgt ttttaactca ggagctggca atcgatttag
     3181 gaacggctaa cactatcatt ctttttaacg ataaaatagt tgtcgatcag ccttccatag
     3241 tggcaattga tcactcaacc gggaaaatga tagccatagg agaacaggcc cgggctatgc
     3301 atggtaaaac ccacgagaac atcagaacca ttcgcccgct cagggatgga gtaattgccg
     3361 attttaatgc ggctgaacaa atgattcgtg ggatgattag catgattaat accaaaaacc
     3421 caatttttac tccatcgctc cgcatggtta taggtattcc gagtggcagt actgaagttg
     3481 aaattcgtgc tgtacgcgac tcagctgaac atgctggtgg tcgtgatgtt tacctgattt
     3541 acgaacccat ggcaaccgcc cttggtatcg ggctcgatgt tatggctcct gagggtagta
     3601 tggtggtgga cataggtggt ggtaccaccg aaattgcggt tattgccttg ggaggtattg
     3661 tatgcaacaa gtcggttcgt attgccggcg atggctttac agccgacatt caggcataca
     3721 tgcgtcagca gcacaacgta aaaattggtg agcgtaccgc tgaggatatc aagataaatg
     3781 tgggttcagc tcttcctgac ctggacaacc ctccggctga ctttattgtt cgtgggccaa
     3841 acctaatgac agcattaccc attgaggtac ctgtttctta tcaggaaata gcttactgtt
     3901 tggataaatc gctttcaaaa atagaatcgg ctatttttaa cgtgctagaa caaaccccac
     3961 ctgaacttta tgccgatatt gttcaaaagg gtatatactt ggctggtggt ggagcattac
     4021 tccgtggcct cgataaacgg ttaaccgaga aagtgaacat tcctttccac gttgctgagg
     4081 atcctttaca tgcggttgca aggggtacca gtatagcatt gaaaaatctg tcgttcccat
     4141 tcctgatgag gtaactcaag cgatgctttt gcgatgaata acctgtacga gattctaaag
     4201 cgcctttaca ttcatctgat attcctaatt ctattaggga tatcgctttc gctatacttt
     4261 aacactacgt actaccagaa atcgcaggtt ggggcagtaa ctaagtcaat ttccggactc
     4321 tggcataagc aagcatcaca ggtaacaggc cttttccaac tgcagcgttc aaacgtacta
     4381 ctcctactgg aaaacgtcag gctccgaaac gaaatagagg ggtacaggct taagctgcaa
     4441 atgcttcagg attcattcat gagaattgag gacacttccg gtttattccg gtatattcca
     4501 gccagggtta ttgataactc aattaacagc cgccataact acatcaccct cgatgcggga
     4561 tcaaaagacg gtgcttacaa ggagatgggc gttatatgta agaacggcgt tgttggcatt
     4621 gtatcggcta ccactaaaca ttttgcctca gtcatttcac tgttaaatac cgatgtaagg
     4681 gtttcggcta tgcataagaa gtcgggcgct tttggagcgg tttcgtggga tggcatgaat
     4741 tacaggcgtg ttgtacttga tgaaattccc cttcatgtta atgtggcagt tggcgatacc
     4801 attgtaacca gtggcttttc aaccatcttc cccagggata tcccaatagg tcgtgttgtt
     4861 gatttttaca ataaggatgg tagtttccta caaattacgg ttgaactctt cgccgatttt
     4921 aaaaccctcg actatgtgta cttggtcgat tcaaaggtga aaagcgaggt tgatcgtttg
     4981 caaacaatga agtgatgtac agcgatattc taaaatattt aggtttaacg gtaatactgc
     5041 ttttactgca gtttctggtg cttaataaca tcaatttcat gggttatgta aacccgtata
     5101 tctacatact tattatttta ttgcttccct accgaattac ccgctggatg ttgcttatta
     5161 ttggttttgc actaggattt gctgtggatt atttttccaa cacacttgga ctccatacca
     5221 ccgcttcgct ggttctagcc tattttaggc cagtaatttt aaaccgatgg agttttaaat
     5281 cggttcagga tatcaggggt gtccccgatg ttacgaacac ctcactggag tggtttgttg
     5341 tatatgccct aattgctgtg ctagttcatc actttactct attcgttctg gaagtattta
     5401 cgtttaaaca cttctggtta acgctggttc gaattattct cagtactgct gtttcaacgt
     5461 tttttatagt gatcattgag cttttacggg ctcccaataa aatgtcaagg tagtggtgtt
     5521 acgagctgaa agtagcagac gtgttgtagt gtcggtaatc ataatgcttg tttttgctgc
     5581 cataattggc aagttatttt atattcaggt aattgatagt tcctacaagt tctcggcagc
     5641 aaataacgtg ctccgctaca tcacacagta tcctgcccga ggactagttc tcgatcggca
     5701 tggagtgccc atggtatcca ataagccggt ttttgatatt ttaatggtaa agaggcaggt
     5761 aaaaccattt gatacacttg agtttgccac tatgcttggg cttacggttg aacaggttcg
     5821 cgatgcattt attcaggcaa caaaacaaaa aggctattcc ccccgtaaag ccgtggtttt
     5881 gcttaagcaa atctctgccg aaaattttgc ccgtttccag gaggtgatgt ataagtatca
     5941 gggttttgaa attcaacccc gtacggtgcg ttcatataat ccacccattg cggcgcatct
     6001 gctagggtat gtaggtgagg ttgatgatcg cgatattaag aataacccat actaccaaca
     6061 gggcgattac attggtataa gcggtattga gcgctcctat gaacatattc tacgtgggaa
     6121 aaagggtgta aaaatcttca tggtcgatgt tcataaccgt atcaagggtt catacgagca
     6181 ggggaaattt gatacgttag caatacccgg taagaacatt accagtacta ttgatgcaag
     6241 gcttcaggag tatggcgaac gcttaatgca aaataaaata ggcagcattg ttgccattga
     6301 acccaaaacc ggcgaggtgc tggctatgat atcgagccca acatacgatc ccaacctgtt
     6361 ggttggaagg caaaggactg ctaacttcaa ggttctccaa caggattcgt taaagccaat
     6421 attcaaccgt tcaatcatgg cgctataccc gcccggttca acattcaagg tggtaaatgc
     6481 gttaatcgga ttacaggaag gggttgttac gcctgaaacc cgatatagct gctatggagg
     6541 atacaccgtg ggacgtggtg ttggttgcca taaccatcca tcgcctacca acctgataca
     6601 atctatacag gtttcgtgta atacctatta ctgccatgtt ttccggaaca ttatcgataa
     6661 aagggaattt ggttcggttg aaaatggatt ctcggtatgg aaaaggcatg tggaatcgtt
     6721 tggttacggc aacaagctga atatcgattt accgcatgag ctaaagggta tagtaccttc
     6781 ggtttcattt tacgataagt actttagaaa gggaggctgg aattcgctta ccatcatttc
     6841 gcttgctatt gggcagggtg agctttccgt tactccctta caaatggcaa accttgtggc
     6901 aacaattgcc aacaagggat actacattgc accccatgtg gtaaaatcaa tagagggcga
     6961 taccttaaac tccctgtacc gggaaaagca tatcacctca attgagccaa agtactttga
     7021 ttacattatt gagggaatgg atttggcagt aaacggagcg ccgggtagcg gaagcactgc
     7081 gcgaatagct gctttgcccg atattcgtat ttgcggtaaa accggaacag cccaaaaccc
     7141 tcatggtaag gatcactcag ttttctttgc atttgcacct aaggacgatc ccaaaattgc
     7201 aatttcggtt tatattgaga atgctggctt tggcgctaca tgggctgcac ctattgcaag
     7261 tttgatgatt gagaagtacc tgaaggattc tatttcccgt cccgatttgg aacgatatat
     7321 acttgacgca aacctattag atagacgtgc aaaggcgaaa taacatacta cgaaacctcg
     7381 attgggttgc aaccatcctg tacgttgttc tcgtattgat gggatggctg aatatttatg
     7441 ccgctgtcta tagcgatgag cacagaaata tcctcgattt cagccagcgc tatggcaaac
     7501 agctggtttg gatattcttt gcattcctca tggcaattgt tattctttac tccaacgata
     7561 gaatctatcc ggcatttgct tatatctttt atgggattat tctgataacg ctgatttcaa
     7621 cgttactttt tggccgtgtg gttaatgcgt caaagtcatg gatacccata ggttcattcg
     7681 gattgcaacc tgctgagttt gcaaagattg ccactgccct tgctcttgca aagcttatga
     7741 gtagctatgg ttttaaactt tcagggtttt ggtcgtacgt taaggtggca gttattattg
     7801 ggttgcctgt tggcattata cttttgcaga acgatactgg ctcggcctta gtttttggag
     7861 ctttgatttt ggttttatac cgtgagggct tgcctgcatg gatattatcg tacctggttt
     7921 ttctggtaat tgttttcatt ttaactctcg tttatagcac tcctgtttta attactttca
     7981 tttttgttgt agcagtaaca ctttttgtag tatttactcg ccagcaccgc gatgcgttca
     8041 gaatatccat tggcgcagca ggaataggtc tgttgctcta catttcgtcg gaactattcc
     8101 atttaaggct atctaatgca ttgatctttc ttatcccagt acttattagt gctgttttag
     8161 gctttttgta tgcttacaag aacaggctaa ggcaggttta tgcaatttca ttctttctta
     8221 ttgcaaccat aggtttttca atgtcggccg attacgtttt tgagcgtgtg ctagatgtcc
     8281 atcaccaaaa gcgtattaac gatttacttg gtattgaaga tgatccgtta gggtgggggt
     8341 acaatgttaa ccaatcaaag attgccattg gctccggagg tttctggggt aaaggttacc
     8401 tttcgggcac tcaaaccaaa ttcaactttg tgcccgagca aagcaccgat tttatttttt
     8461 gcacggtggg tgaggagtgg ggctttttgg gttctactgt tgtggttttg cttatagcgc
     8521 ttttcctgtt aaggctacta aaaattgccg agcgtcaaac cgaacatttt gcccgtatat
     8581 acggatatgg agtagtttcc atcctgtttt tccacgctgc ggttaatatt ggcatgacca
     8641 ttgggttggt tcctgttatt gggattcccc tgccgttctt tagctatggg ggctcgtcgc
     8701 tttgggcgtt tacaatcctg gtatttattc tccttcggtt cgatgcttca cgctatgggg
     8761 catactaata ggtataccgt attctttttc tgcaacctgt attaatgcgt aaagcaattc
     8821 ctgaagccca gcactgaacg caatatagtt ttccttcact atttgcagag caaggcttgg
     8881 gttccctttc attgtggtgt agttacccat tatctcaagg gctctggtta caccctcatc
     8941 ggtgctgtag gagtagagtc tgttgctttg aatcataaat ggtaagaatc cccttactgc
     9001 tgggggtagc atgtggtagc gctgaagcat ctgaaagtag aaagttcttg taaagccttt
     9061 aagaggcctg tttgagtatg tactccattc tttagctagg atgtaatcga acatcacatc
     9121 tgtagcaaca cccgaccatc gcccatagct ggggcgcaat ttctccctac agcttttcca
     9181 tgcctcgtgc ctatcagtaa cctgatcgat tgtgcggtga agctttatgc cagtttgaac
     9241 accctgcggg taggcaagga tttgtttccc ttttacatga tcgcctataa aattgcccaa
     9301 ccgaatatcg atgttgctcc ccgaaaggta taaatgagcc aaaaaattca ttaagtagct
     9361 tgttataaga aagttgtagt agccaaaacc tcatcggctt tgggtggttg gtagcgtata
     9421 agctttagct ccttgccatc ccaaacactg taggtgttgc ttactaacca atcgccaagc
     9481 actacaaggt tcgatttttc tgaaagcgga tatattatgg gtgaatgcca gtgcccataa
     9541 ataaaaaaat caaaatgttc gtcctggagt ttatgccttg agaacttggt tatttgctca
     9601 tcctcgcccc tgaaaacatg ggttatttcc ttggagtatc tactgcttac actccactgg
     9661 ttaccaaacc ataacgagaa gttagggtga agtttggcaa acatccattg cagggtaggg
     9721 ctagtaaaga ttcgtttaag cagtttgtag ccgggatcgc cagggccaag gccatcgcca
     9781 tgggcaagca ggaactttgc accataaagt tcccgaacaa ttggttcgcg gtgaatttga
     9841 acccctagtt ccttttcaaa atagtcgaac atccatacat cgtggttgcc agtaaaaaag
     9901 tgaacgggaa tgccgctatc ggtaagctgg gcaatttttc ccaggaatcg cgtatatcct
     9961 ttgggaacaa ccctcctgta ctcaaaccag aagtcaaaaa tgtcgcctaa aaggtatact
    10021 tcctcagcat cgtggctaat ggtatcgagc caggtattga aaagcttctc acggggtagg
    10081 ctctgctcca ggttgggcaa gcccaaatgc agatccgatg caaagtatat ctttttaccc
    10141 tggctcataa tgttttttac ttgttcaggt agctatccaa ggccttttgc aggtttgtgc
    10201 cgtatagatt ttttccaatg atattgaaat ctttaccaat aagataattg gcaggtagtt
    10261 gtctaacgtt gtagaataaa gcggcatttg aagccgatgg atcatcctcc cttacgctaa
    10321 tccacggtaa tccggcgttc tcaatgtagt taatccaacc ctcacgggta gcatcgaacg
    10381 atatctggta aacctcaaac cctctgcttt tatacttctt ataaatttcc aatagctcac
    10441 gattatccag caaacttgca gtgttctcgc tggtaaaaaa gtcgagaagt ataaccttac
    10501 cctttagcga tgatagttta atgctacgcc cattcctatc aggaatgctt aggtcaggaa
    10561 taggcatctc agcattctgg ataagttgct gcattttagc attggcaatg cgattttcaa
    10621 tattcttgat atcctcaagc atccctttgg tgtagctcga ttccgggtaa agcgctttcc
    10681 acgcggtagc aaccattttt attagcagta aatcgtcgga gctgtcaaaa atgtacaggt
    10741 tgtcgttata tttttggtat agtgccatta tgctggcttt gttcatgggg ttctcccaaa
    10801 caaatttggt gagttcgtcc tttacccgct tgcgttcctg ctcaaattgg ttgtttatta
    10861 attcacgttc cgagttactc tcggcgttca ggtatcgttt tgatagctcg tcaactgcgg
    10921 tttttgactt ggcaaatgtt cttgtaaccc aacgaacctt ttcagaatcc tcagaacccg
    10981 aaacctgata gtcgatggca ttgttaaggt ctaatattac gttgattttt tcaccttttg
    11041 aagctaatag ggtaatggca agccctttgc tagtgctaag ggagtaaaac tccggttctt
    11101 cgccctgttt taccttaaac ttttttacac tttcgccagc cgaaatccta accgagtcaa
    11161 gctttatggt tttattgaaa tcaattttac ttaagaaaag tgtagttttt tcaggactct
    11221 ttacttttac gctaattaca accgcatctt tgctgctgca tcccgccaaa aacactatta
    11281 aaactattga aacaaaatac ctaaaacgca tgctgtttaa attttggtta catgcaaatt
    11341 tagccaaact ttacaatttt ggcttaccca atgtctatct aatgcttttt taattgcatt
    11401 tttgtagata ttttgcttaa actcttggtt tatgagagca aaacccgatg gttcatatat
    11461 aagctatttc agtaacctgg taaaagtaaa tggaggtatc aaccttgcac agggcattcc
    11521 aggctttcag cctcccactg aactcattaa tattttaaaa gatattgcta attcgcagca
    11581 tcaccagtat gcgcctggaa ttggtaatct gcagcttaga gcgctaattg agaatagcta
    11641 tggagttgaa cccgatgagc ttctggtagt tcagggagca accgaaggcc tttcgttggt
    11701 ttacacctat ctttttcgaa tgcttggaaa aaatctggta accctttcct ttgaccctgc
    11761 ttacgaaagc tacagtcggc tgcctcaaat cttcaaccaa aaatttgttc ccttttcttt
    11821 gattaatgaa gagtttgttg acttcgacga acttaaaaga tgcattgagg aaaataaggt
    11881 aaagttgata ttcattagtt cacccggcaa cccctacggt aaagtttgga caaaagcaga
    11941 aacctgtaaa cttctcgaac ttgctaacga gctaaatttt tttgttgttt tcgattcggt
    12001 ttatgaggag ctatactttt cacaaaaacc ctatattcct ttggataata tctccgaaaa
    12061 gttattcatt gttagcagtt tttcaaaatc gttgtgtata acaggatggc gtgtgggtta
    12121 tgtaatccat catcccagtc acaccaaggc cattagggcc attcacgact atatcgggct
    12181 gtgtgcacca agcgttatgc aacaagcgct tgcggtttac cttgcacagt tccaatacgg
    12241 taaagctttt atggatgagc taaggcaaag ggttaagagt tccttcgaca gcttaagtgg
    12301 tatgcttaac cgtttaaact ttacggttcc caaaattgat ggcggttgtt ttatctgggc
    12361 taagttgcct caacccttta ccgatggctt tgagtttgct tctgaccttt acgatgctac
    12421 tggcgtggct gttattccgg gcgagcattt tagcagcaca aaggtaaact ggttgcgctt
    12481 taatgtggct cgacctttag aggagataga tttagccggt aatagaattg ctgattttat
    12541 gagcaagcaa tgattataat ctcaaccata ctgattgcta tttacactat agccattcta
    12601 tggttactaa aaggtttgtt gaccaataac tggttggaat ccaatggagc ccccagtcaa
    12661 actgttaatg gcatttcggt gatagtggca ttcaggaatg agcgcgaaaa tctacctaaa
    12721 cttattgctt cgcttgaggc tcaggattta ccgcatgagt tttgggaagc tattttagtt
    12781 gatgatggct caaccgatag cggttttgac ctagtaatgt catgcaaaag ccaattacct
    12841 ttaaaacctt tggttttacc tgatgggcag gcaggtaaaa aacaggcatt actttatgga
    12901 ctatcagcag ccagttttga tactgttgta ttcaccgatg cggattgcac ccacactcct
    12961 cagtggctaa gctctattcg tgaaagcatg gggagtactg attttttaca gggtgaggta
    13021 aaaccccagt ttgaaaaggg cagtattatt tccatgttcg aggcactcga ttacctatcg
    13081 cttatggccg tttcagcggc aagttttgct gttggcaaac cggttatagc ctcatcggca
    13141 aatttggctt tcaggcgaag caaggttaat gtagatacta aggtattgcg caccgatata
    13201 gcctcaggcg acgatatgtt tctactgcat catgccaaaa ccattactga tttacggttt
    13261 aggttcttag catcgcctag ggtggctgcc tacacaagtt ttagcggcgg gataaagggt
    13321 tttgttttgc gccgaagtcg ttgggcatca aaggcaaccg catataccga ttccctaacg
    13381 ctactattat cgttcctggt ttttaccctg aatttttgga ttattctttt agcaatacta
    13441 acagttttgg gtgtaattac ttttaacctt ccaatttcac tccttgctgc caaagcaata
    13501 gtagatttac ctttcttggc atattacctt tttaaaacca aacagcaaaa cctattggcg
    13561 gtttttatac ctttacaaat cctttatccc ttttatataa catttgcagc cattctagga
    13621 ttaattaaag gaacaacttg gaaaggaagg ataaacagct agcggagtta gcccgaagcg
    13681 gttccccaat gcgaatagcc atacgtaggt tacttgccga ccgaatggct gtagcatcac
    13741 tattgcttat agcctctatt gccattatag cgtttcttgg atacctaatc atacccgata
    13801 gttcgccata tgcaaatggt cagcatttac agctggcaac gcttaagcct ggcagtaaag
    13861 tcaaattcct gcgtgttgct aagcagcacg aggtaaaaaa gcgaaatatt ctgggccgaa
    13921 tgttttatgg gcaggaacag gattacaacg agatacctgt tgattcaatc tactttgatg
    13981 gtattagggt atatgccagt gagtacaacc cgattcccga aggcgatgcc attgttgccg
    14041 aattaactct acccgaaatc ctcttcaggt taaaccccga aaaaaaatac ttacttgaga
    14101 acgatacaat cactgcattt ctgtacaatg ggcagcatgc aattatggct gtagccgatg
    14161 cccaacaaag ggtattaagt caaaaccttg taacgcgtag gtttcttctt ggaaccgatc
    14221 gttttggtcg cgacctttta agccgcttgg ttattggtgc gcgcatttcg ctttctgtag
    14281 ggtttattgc cgtgggtatt gctttaataa ttggcattgt actgggagcc attgccggtt
    14341 ttgcccgtgg aattattgac gatgctataa tgtggatggt gaatgtggtt tggtcagttc
    14401 ctaccctgtt aatggtaatt gccttaacca tggtaattgg aaagggtttc tggcaaatat
    14461 tcgttgcagt tggcttaacc atgtgggtgg atatggctcg cgttgttcgc gggcaggtgc
    14521 tcagcgttag ggaaaaggag tatgttgagg cggctaaggt tctaggttac ggcccgtggc
    14581 gtatcatttt caggcatgta cttcccaata tcatggcacc ggtaatcata gtttcggcaa
    14641 gtaacttttc aacggccata ctgcttgaag ccggattaag ctttttaggg attggcgtgc
    14701 aacctccagc tccctcgtgg ggtactatga ttcgtgacca ttacggctac atcattatgg
    14761 ataaggctta cttggcactt atacctggct ttgccatgat gcttttggta ctggccttta
    14821 acctgcttgg caatagcctc cgcgatgccc ttgacccccg tgaaatagaa aaggcctagt
    14881 tggctaggcc ttaaatctat attgtaaatg gaagtattta aaacgatgat aagaaactat
    14941 gtaattcgtt aatattttta aaatatgtaa aagtaatttc atcagtatca gtttttatat
    15001 cttttttaga aatccgtgat actgcgttaa gcatttcttc aatgttttta ttagcattca
    15061 gtattgaata atctatatgg gaataagtaa caaatttata aaactcatcg gcaaaagaac
    15121 gagaaataaa gaaaatacca gaaaagtcaa aaatatattt acagttatta tcaaaggaaa
    15181 ttgattcacg gatttccttt atagccttcc ttgttgatat aatcgaaggc aaatattctc
    15241 taatatgaat gactttggtt ttcatattac aaaattaaaa aaattatcat ctttttacaa
    15301 agaaaaagtg ttaaaaatgt ttatgaatag cgctttttat ttgaattttt gtaatttatt
    15361 tattcaaggt aattcgtgta attgaagttt gagggtatgt catggggtat tctaacagca
    15421 acaattgttc ctttccattt atatggaatg tctgagaaaa ttgtttcaat attagtgttc
    15481 caaatgtaga aagaatttcc tgaaaagagc aagaacatac ctccaagtcc ttccactaac
    15541 attttccttg aagttttaag accataccct cttgtattac cttgttcttt tgtgcttaag
    15601 ccattgatag ccttatttat ggcttgtgca tctgttgaaa tattaaagat ttttgttttt
    15661 ttataagatt caagtatacc atcgccagta tctgcgatgc atacatccaa aaaacctttt
    15721 tttggatagt tttgagccat aatccaacca tttgacacgt tggcgtgctc aacgatattg
    15781 tcaatcgttt cattaattat atagctaaca gcaattttaa gttgaccctt gatgttagtc
    15841 tgacttacaa gtatagtttc aaagagggaa aggatttggt ctctaatttt tgaattattt
    15901 gacccagaag gtattgaaca aataggtaaa taagttttgt ttttatattt tttcaattca
    15961 atagttaatt cattgtaatc gtatgataga ccatatggaa acttaattac atttaagtat
    16021 ttctgagtgt ttttgttgta aacaaaatca atgtcataat aaagggatga gagatatttt
    16081 aaatatgatg caattggaag taatgtcaat ggataagtaa agtcaatatt ttgcaaatta
    16141 attataagtt tcgatccctt atcccatttt tgaatttgac ttaaaaagtc aattaaaacc
    16201 tccagagatg cacccaaatt gtaattagca ggttttttga caataatttc aatttgcaca
    16261 actaaattgt ttagggtttt atatgaatta aaaggagaat atagaagata aatttagtta
    16321 atttaaaaga taaagtaaaa taaaatgagg tttggatttt ccaaacctca ttgtctaata
    16381 acctactcca cccaccgttt tacatcgtcc atggtggggt tgggcagacc taacttattc
    16441 tttttgttgt agccgcaaag gtcgttgaaa agtttcccgg ctgcaactcc gttcagagag
    16501 gccacggtgt taaaccccat tttttgcaga actggaaccc agtcggcggg aatgcctttt
    16561 tgggcgtagg cttctgcagt gtccttctgc ggtttctttt cggggcgcat ttgtgggaag
    16621 aatagtacat cctgtatgga tggctggttg gtcatgaaca tcgttaggcg gtcgatacca
    16681 atacccattc ccgagcaggt tggcatacca tactccaggg ctctaacaaa gtccatatcg
    16741 atatacatgg cctcatcgtc gccgcgttcg cttaggcgga gctgctcctg gaaacgctcc
    16801 agctgatcga tggggtcgtt cagctcgctg taggcattgc aaagctcctt gccagcaaca
    16861 aatagttcaa agcgctcggt tagctcgggg ttgttgcggt ggcgcttgca cagcggcgac
    16921 atctcaatgg ggtaatccat tataaaggtt ggctgaacaa gatgctcctc gcacttttcg
    16981 ccaaaaatgg catcaatcag ttttcccttc cccatggtat cgtcaatctc aatctccatc
    17041 tgcttgcaaa tggcacggag ttcatcttcg gacttgccag taatatcaac gccagcatac
    17101 tgcttaatgg catcggacat ggtcatacgg cggaatgggc gcttaaagtc aatctcctta
    17161 tcgccaaagg ttaccttggt cgtgccatga atggcaacgg ctactcgctc aagcatctcc
    17221 tcggtgaatt ccatcatcca aaagtagtcc ttgtaggcca cgtaaatttc cataacggta
    17281 aactcagggt tatgggtcct gtccattccc tcgtttcgga aatcttttgc aaactcatat
    17341 actccattaa agccgcctac aatcaatctt ttaagataaa gttcattggc tatgcggagg
    17401 tataagggga tatcaagtgc attgtgatgc gtaacaaacg gacgggctgc tgcaccaccc
    17461 ggtatgggtt gaagaatggg tgtttctacc tcaaggtatc ccttggaatt aaaaaactcg
    17521 cgcatggtgt ttattatggt agcccgcttg cggaaaacat cgcgcacacc cggattgaca
    17581 attaggtcga cgtagcgttg gcggtaccgg agttcggggt cggtaacggc gtcaaacacc
    17641 ttgccgtcct tttctttaac tattgggagc ggccgtattg atttggcgag cagtgtaagc
    17701 tccagggcat gtaccgattc ctcacccatg ttggttcgga atacaaatcc cttaacgcct
    17761 acaatatcac ctatatcgag tagctttttg aaaacagtgt tgtacatggt tttatcctcg
    17821 ccggggcaaa tatcgtcgcg tttcacgtaa acctgtattt tacccttctc gtcctgtaat
    17881 tcaaaaaagg atgcgcttcc catgatacgg cgagccataa ttctacccgc aatataaacc
    17941 tctgcccggt tttggctcat gttctcaaaa tccgatagga tatcggcagt ggaatgggtt
    18001 acgggataca tggcggcagg gtagggttca atccctagac ttcgtaattc atcaagcgct
    18061 tgtcggcgaa atatttcctg ttcgctaagt tcttcgtata aagccattgt tttatctgat
    18121 tttttgcaac ttgcaaaaat acaagaattt tgctaatcga cactttaact ctttgaatta
    18181 tcaaaaacat gttttcaggt aaattaacaa aactattctc ctttatttag ttttctgttt
    18241 tacttttgcc atcagaaaac taaaatagtt taaatagttt tatggcggtt aaggagcaga
    18301 tagtagaagg ggcactggaa ctattcaagc gaatgggaat tcgtgggatt accatggaca
    18361 tgctggcaga gcacctagga atttccaagc gaaccatcta tgaaaatttc cagaacaagg
    18421 atgaaatagt tgaggcctgt gttttactcg agaatgaaag tatgcaggcc atggtccaaa
    18481 gcattttgaa caaaagtgag cattacattg agacctatat catgttcatg tggtcgcata
    18541 tcaataagtt acgcaatgtt agcccacttt tcatgtacga tttaaaaaaa aattacccca
    18601 aaaccatttg taagcaagca ggagagtttg accattactt gaaggagaga atggttgagt
    18661 ttattgaaaa gggtaaggcc gataatcttt ttagagctga agtaaatcca caaatcgtgg
    18721 ccagcgtggt ttttgaattt acaaaactcc ttcgtagcgg atttgaaccg ggctccaact
    18781 ccttaaacga ttttcctttg gccgaagttt ttgaacacat ggccgtaaat tttattcgcg
    18841 ggcttgccac ttcaaaagga attgaactgg ttgattatta ctacgaaaaa cacaaacaaa
    18901 ttcgataatg aactttaaac attaacagta tgcgtaagct aataacaatt ttttctcttg
    18961 caggctttgt cctttcaaca agcctagccc ttgggcagca aaagcccgac acattgcatt
    19021 taactgttaa gcaggcggtt gaccttgcta ttcagcaaaa cctaatgcat aaggtaactg
    19081 aacttgaact gcaaaagaaa caggaaaagg taaaagagta tatggctgcc ctttatccaa
    19141 acattaaggc atcggctagt tacacgcgaa atctcaaact ccctgtaata tttatgcctg
    19201 aaggttcgcc ctttgggtcc accttaacca ttggctccga taatagttat tctgcttcaa
    19261 ttagcgcatc aatgccattg tttgtttaca atgtatttga gtcgattcgg ttaggacaaa
    19321 aggatgttga aataagtcag gaaaagttgc gcgagagcaa aattaacctg gccgctaaca
    19381 tcaaaacaac gtattacaat gttctgttgc ttcgccgaac acgcgatgtg attaaccaaa
    19441 gttaccagaa tgccgttgca aactttaata atatccaaaa gcttaatgcc aatggcatgg
    19501 tttccgacta cgactttatc cgcattaagg ttcaggttga taatatttat cccaatctgg
    19561 ttcaaaccga gaatgcctac accaacttac ttaatatatt caaggtgtta ctcaatatcg
    19621 acataaatac cccagttgaa ctcgacgaaa aggaacttac caactacaat ttcagtgaga
    19681 tggcacttgc cgattccagc tggttgagca gtaactcaac cctaaggcaa cttaccctta
    19741 cccgacaaat gctatccatt caggagaaaa tggtaaaggg tgcaaattac ccaagcctgg
    19801 tggcaattgg taattaccag tatcaaaccc aggcaaacga ttacaaattc agcaactaca
    19861 attgggtgcc aacatcgatg gttggtttgc aactcaatgt gccaattttt agtggttttt
    19921 cggttcgtaa acagctcagt caagtacgca taagccagca tcaagttgat atgcaagcgc
    19981 agtttacgca aaacaacctg acggttcagg ttcaaaatgc tgtgaatgct gtgaatgctg
    20041 cagtaaaaaa ggtgaactcg gcaaaaggta atgttgacct ggcggaaaaa ggatatacta
    20101 ttgctcaaac ccgctacaat actggtcaag caacgctttt ggagttgaac gatgccgaaa
    20161 atgcaatgct tcaggctaag ttaaatctaa tccaggcaca gttggagtac cttgtggcta
    20221 aaaccgatta cgaaaaaatt atcggtgaat cattttaaaa gtaaaaaaat ataacgcaaa
    20281 atacactatt actatgagaa cgattaatac ctttaaagca attacgcttg cctctgcagt
    20341 agttttactt gcctcgtgtc agggaaatag ggcagatgaa aaacaacttg ccaccactgc
    20401 taccgaagta aaagtggtta ctcaggttgt acaacccgtt agcatcaatc gccagtttag
    20461 gtatcctgca accattcagg cttacaagga aaatcacctg gcgcctgcaa ctgccggaag
    20521 ggtagaaaag gttttggttg atgtgggcga tagggtaaaa gccggcgatg ttgtagccat
    20581 gctcgatcgc acaacctatc agcaagctaa aatccagttt gaaaaactta aaattgacct
    20641 gcaacgtgta gattcgttgc taaaagtagg agcaatacca caacagcagt acgatgctgt
    20701 aaaaatgcag tacgatattg ccaaaacgaa ccttgagttt cttgacgata atacctcgtt
    20761 aaaatcgcct attaacggaa ttattactgg taaatacatt aacgatgggg agctatttac
    20821 catgtcgcct gctccgggtg tagggaagcc tgcagtggta tcaatcatgc agctcgataa
    20881 gcttaaactt ctggttgggg tatcatcaac ctatttaaat gcagtaaagc cgggtatgaa
    20941 ggttaccgtt aaaaccgaac tttaccccga taaggagttt acaggtacag tagagaaagt
    21001 ttaccctacc atcgataata tgaccaaaac ctttaccatt gaggtggttg taccaaaccc
    21061 cgataagatg ctgaggccag gaatgtttgc tactgcctac ataaatgtgg gtgtgggtga
    21121 tgcactgctt gtgccagcgt ttgtggttct aaaacaatcg ggaaccaatg aacgatatgt
    21181 gtttgtttac gataacggga aagccatccg taggaacata gagcaaggtg atgtgttcga
    21241 cgataaagta gaaattatta aaggcctatc tgccggtgag gagattattg ttgagggaca
    21301 aaccattgtt ttcgatggtc aacccgtggt taaaaagtaa ttcttaattc gacttagtat
    21361 gagtctttac agttcagccg taaaaaatcc gatatctacc ctgatgattt tcgttggggt
    21421 aatcgtaatg ggcatttatt caatatttta catacctatt gacttattgc ccgagatgga
    21481 aatcccttca attagtgtaa ttacattcta tcctggagct agtgctgagg atattgaagt
    21541 aaatgtaacc aaaccaattg aaaatcagct taacgctttg caaaacctca aaaaggtaac
    21601 ctcatcgagc aaggataaca tatcgtttgt tacctgcgag tttgagtatg gtattaacct
    21661 cgatgaggca agtaacgact tacgcgatgc ggtaagcatt gccgaaaact cactccccga
    21721 tggatgtgag aaaccatcaa ttttcaagtt cagctcatcg atgatacctg ttgttatata
    21781 cggaatacag gccgatagga gttatgcagc tctcgaaaaa ttgattgatg agaaaatcat
    21841 tgaaaagctg agtcgtattg atggtgtggg atcagttacc attataggta aacctattcg
    21901 aattattgat gtaactgttg acccccgtaa gcttgatgcc tacaatatta cccttgaaca
    21961 ggtaggagct gttattgggg ctgagaatat ctcgctacct gccggtcgta tcgaagttgg
    22021 tagaatggag tttccggttc gtacaaacgg ggagtttaga aacagcgatg tggttaagag
    22081 ccttgttgtg gccaacaatg gtggcaagct ggtttacatg aaggacattg ctactgttaa
    22141 ggatacccta aaaagtgtgc tggttgatga aagaatagat gggagtttgg gtgttaggct
    22201 tatggttcag aagcaaagcg gagccaacac cgttaaaatt gcccgattgg ttagggaaag
    22261 tatggatgaa ttacaaaaag atctcccttc ggatgtgaag gtggttaaaa ttatcgattc
    22321 atcggaattt attcaaaatt caatcaataa cctttccgaa acgcttatat acgcctttgt
    22381 atttattacc ctggttgtaa ttcttttcct cggtcgttgg cgttccactt tcattattat
    22441 gctcaccatt cctatttcgc ttattggggc tttcatctac ctgtttattt caggcaattc
    22501 cataaacatt gtatcgttat cggctttgtc tattgcaata ggtatggttg ttgacgatgc
    22561 cattgtggtg ctggagaata tcaccactca cattgaacgg ggtagtagcc ccatggaggc
    22621 agcggttttt gccacccgcg aagtaggttt ggcagttatt gctacaaccc ttgttattgt
    22681 tgcggtattc atgccgttaa cccttatagg tggcttaatt ggtgttttct tccgccagct
    22741 gggatggatt gttaccataa ctgtttcgct ttccacatta gctgcactct cgttaacccc
    22801 aatgctttcg tctcgaattc ttaaggggcg ttcgtactat aagcatcatc gcagcaggtt
    22861 cttggaatcc gttaaccgtt tctggggctt ttttgatacc atttatgtaa aatcattggc
    22921 ttggacttta aaacacaagg ctctgacact aattattgcc gggttggttt ttattggaag
    22981 tttaggattg gccggtgtag ttggtacaga gttccttcct tcgcaggata acggtcgcat
    23041 caacgtaaat atggagatac cccagggtat tcggattgat gaggccaaag cgcttgcacg
    23101 tgattttgaa caaaaggtta agaaaaaata tcccgaagtc acattgatat caacaagtat
    23161 tggtgccgat gaagggggat cgttaaagag tgtattccaa aagtcagctt catatatcat
    23221 taattacagc ataatgctca ccagtgctga tgatcgtact agaagcattt tcgaaatagc
    23281 cgatagcctg cgtaaagata taacgggtta cacccaggta acatcgtcgt atgttgaccc
    23341 aggagggaac cgtaattcta ggataacttt aggaatggga ggaggtagta atcttgaggt
    23401 taaaatactt ggttacgata tcaaccaaac caattacatt gctgagcaaa tagccggcac
    23461 tctggaagcc gaaattggat tccgtgacat atcgattagt cgcgataagg atcgtatgga
    23521 acttcaggtt atccccgatc gggagaagct ggctcagctt gggcttaaca cttccatgct
    23581 agcctcagca gtacgtaaca ggatatatgg gctaacagca accaagtata gggaagaggg
    23641 taccgagtac gatgtaattc tcaagtacaa cgatgccaac ctcacctcgg tgtccgatat
    23701 tgagaatatt acctttaaag gtcccatggg taatatggtg cgggtaggtg atgtggccca
    23761 aattaaacag gtttacacaa cccctaacct tgaacatgaa gataagatcc gtgtagtaaa
    23821 ggtggttagt gcactttcgg gtatcgattt gggaaccgct tcaagtatta tcaatgctaa
    23881 gctggctacc ttgaccatac ccgacaacgt gattgttgaa attggcggtg ctgctaagga
    23941 catggccgat acctttcaag atcttatgct gctttttgtg ctgataatgg tgttaaccta
    24001 cttggttatg gcagcacagt ttgaatcgct gcgtgaacca ttcatcatca tgttttcggt
    24061 tccctttgct cttacagggg tgttcatttc cctttatgta accggtacta ccctgaacat
    24121 gatatcgggt attgccattg tgatgttagt gggtattgcc gttaaaaatt ccattgtgct
    24181 ggttgatttc atcaacctgt tagttagtag aggtagaaca atttcccaag ctatactgga
    24241 gggtggaaaa tcgcgtttac gtcctattct gatgacgtcg tttaccacct tgctgggtat
    24301 gcttcctatg gctctttcaa aaggtgaggg ttctgaaata tggcgacctc tggggatatc
    24361 aattgttggt ggacttgtct tttccatgtt gatatcgctg gttattgtgc ccataattta
    24421 tgcccttttt gccaatgctc gtattaaacg cgaacggaag aaactccaag cttctgatag
    24481 tgaaatatct tttgacgatt aacttctcaa tactaacgat atgaaagcga ttttcattgc
    24541 atacaatcag gctctatccg ataaggttga tagtgcttta cagattgctg gagtaaaagg
    24601 attcagcaaa tgggccgaca tgcttggtgc tggtagccaa aaaggtgaac cacatttagg
    24661 tacacacact tggccatcca tgaactccgg catgctaacc atagttgacg atgataaagt
    24721 ggataaactc ctatcgtata taaatcaaat aaacaaagtt gctgaggagc aaggtatcca
    24781 tgcctttgt
//
LOCUS       DAOOWJ010000030        24449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2056_length_24449_cov_8.656760, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000030 DAOOWJ010000000
VERSION     DAOOWJ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 24449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24449
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2056_length_24449_cov_8.656760"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLW22_04615"
     CDS             complement(<1..885)
                     /locus_tag="PLW22_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU74021.1"
                     /translation="MKILRLVSFFGAWLIGIGYVSGQQVLTLEQCRQMALEHNQKVLI
                     ADEHVEAAGALKRSAQTQFLPSISANGLYTRTNKKFSLLDKDLMLPVIPYTAIDPVTG
                     GLNSAILSPTLPDGTPNPNFNPSVFGSTFAIDPSTGQPYTDSDGNPLFQRYTWLPKDK
                     AEFGNKNVYVAGVNLTQPIFTGGKIRETYRIAKYGEKLARAQSEAERTEVLYKTEESY
                     WRVAAVAEKVKLVQAYIALLDKLNTDLENYYAEGIIIKNDLLKVKVKKNEAELNLLKA
                     QNGLTLSKMALCQQVGLLL"
     gene            complement(898..1494)
                     /locus_tag="PLW22_04620"
     CDS             complement(898..1494)
                     /locus_tag="PLW22_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU74022.1"
                     /translation="MNQLEDSHKGMRENIVSAATEVFSRYGFKKTSMEDIARALRMGK
                     SSIYYYFKGKEEIFQAVVDREANLLRVKVKEILESGLPVTEKLRSYVKMRMDLIKQLS
                     NYMEILKNDDLMNLELTEKFRKKYDDEEITIVKQMLEEGINRNELKVKDLNLSALAIV
                     TAMKGLEIPLVTSTMGVESLNMVIDDMLDILFYGIVKR"
     gene            1821..3974
                     /locus_tag="PLW22_04625"
     CDS             1821..3974
                     /locus_tag="PLW22_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOU74023.1"
                     /translation="MDVKEMAKQHKMLNQWKYEWKPLYRGYTDKTLYINVGTNEVKEK
                     PVPPIMKEKFIGGKGYDLRLLWDATKPNTKWNDPENEIVISSGPIGGITQYSGTGKSI
                     VCTISPQTDIVIDSNVGGFFGPFLKFSGFDALEIQGKAKNDVIIYIDGVEHKVEIFEA
                     PAEPLDSHILGEVLSEMFANDEADKKNIGIVSTGAAADHSLIGMLNFTFYDPKRKCIR
                     LKQAGRGGIGTVFRDKKIKAIVCKIPGVKGNLNNVADLEAIAERGRRFNREIRELDDK
                     QCQMRSKGTAHLVEIMNDYDLLPTHNFQYGSHKDAHKIHSDVWKSYFTQGVPDGCWIG
                     CNMSCAKGVDKFEIKTGPYKGQKVIVDGPEYETAAGLGSNCGIFDPEYVIEANFYCDT
                     YGICTITWGTTMAFIMECYERGILNKERTGGLELRFGNKDAALELLHQVARGEGFGKL
                     AGMGVKKLKEHFVKEYGADEKFLNDIAMVNKGLEYSQYVSKESLAQQGGYAMTNKGPQ
                     HDEAWLIFMDMVNNHIPTFEDKAEALHYFPMFRTWFGLQGLCKLPWNDVEPENNAETD
                     EPAKVPEHVDNYVTIYNAVTGSNIDKHELIRQSERVYNFQRIFNIRRGYGTRKYDAQP
                     YRAAGPVTKEEYLSREERYDKQLKEQVGVDPTKMTLEEKMAALRKYREDRYEKLLDAV
                     YERRGWNKNGVPTIEHLKKIGMDLPELIEVVKPLQ"
     gene            complement(4413..5492)
                     /locus_tag="PLW22_04630"
     CDS             complement(4413..5492)
                     /locus_tag="PLW22_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74024.1"
                     /translation="MRKNITKILSALGFITISAACSYLPRTWIDALSGASITLSQNGN
                     SLFHQTDAKKLNISEISIQGEVKKTGVVDLRKFYKREVFVKEAILDESNDPKFIGAFR
                     YIGYSLFDLLNSYNLDKKNEKFFRPSIDVYVVISNDKGEHVSFSWSEIFHTINPHQII
                     IATEVAQIKPHKKEVNYLTGNCWKVVAANDLFSYRTIENPTSITIKSFDKKEYPINRE
                     IKPMYSPAVNVVFDNGFDFSIPTISDTSRIIKYTSIFVGMGMGYHPNPEFKGIPLKSL
                     MKKPNNLFDREWIMNGLVCFSSIDGYRAIYSYSELFNRSDQVIPILSIPENVEDGGYY
                     RIYLPTDFFADRSVKSLKEIYFFKD"
     gene            5685..7994
                     /locus_tag="PLW22_04635"
     CDS             5685..7994
                     /locus_tag="PLW22_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU74025.1"
                     /translation="MKKTFLFFAMAFFSLNTFSQVSLSGKVIDKETGNGIPFAIVEVK
                     DSDIKTVSQSNGAFLLQITGNNTIEITAKSLGYSSKSLVISPDSFNKEITIMLDAEIY
                     ELKTVVVTATRSERKILDVPQRVEVIGNNKIQGIPALSADNYLYSIPGVSISRGASIF
                     GAADISLRGMGNEAGRTLVMIDGAPINKNDGGTVNWNSINTANLKQIEVLKGSGSTIH
                     GGNAMGGVINLITPTPSEPLQGDVSLSYGSFSTLQTQANVSGIKDRLYWSINGSHRKS
                     DGYITSQADEVDEFSIPSFLTEYNVGAKVGFQLSAKHKIECSGGYYSGQRGTGSNFTG
                     YGFTNENLASSKGAYNWYTDINSRVSYQGIFNDNSNLKVNFYTQREYYKNIRESLKNN
                     EIKRYDVLSTRDDIGLLSGYSFSLGKYNKLTSGIDMRYGAVDGADTYITSTDKVLNLG
                     KMNLVGVYLLDEIKIGETPLSILAGIRYDYAMFYDGKFLVENPTSETAFLQNFSGDLN
                     NANFSAFSPRVSFQYYQPQKFRTYLGYSRGYRAPVLDDLCRTGRISGGMKIANPLLKP
                     EYIDNYEIGADILYFKKVSFSTSFFYSIGQDYHAYIATGDSIILNNKLRPIMTKSNIG
                     KVEIYGAEFNVNFTLSNSFIWSLAYSHIVTNILDYKRLNSEIEDNLVGKELVYQPSDI
                     FNTTLIWKNSIINSSVVFNYKGAQWINDINTEKINSYYFIDLQLWRPIYKGLQASLMI
                     HNLLNNHYIDSRNMISPGRMLSLQIKYEF"
     gene            complement(9178..9642)
                     /locus_tag="PLW22_04640"
     CDS             complement(9178..9642)
                     /locus_tag="PLW22_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74026.1"
                     /translation="MERIQIYKKQKGLEIYVKIFGLLFILFGMTFLIKSLMNGDKTDF
                     NQGDWNYLFYIIQVIQGAALFALGYSNKKNEKLFIEWDEKEMRYLLSKNKTIEKIKFE
                     DIRKVTIQLYEIKIELPSTEKVLNLKNIQYNELKKIKEKFEELKLDLEKRNG"
     gene            9984..11318
                     /locus_tag="PLW22_04645"
     CDS             9984..11318
                     /locus_tag="PLW22_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU74027.1"
                     /translation="MAKILVVDDDSTFCLMLKTFLVRNGHKVTEAFSFDDARQAIQTT
                     TFDVVLTDFRLPEKSGMDVLTEAKRKNRDTIVIVMTGYGDIRMAVKAIKQGAYDYVTK
                     PVNPEEILATINNALPISVGKTPETDTDAFTFIEGKSRYSQEVMRYINIVAPTNLSVI
                     IQGDSGTGKEFVARQIHRASKRNSKPFVAIDCGALPRELAASEFFGHIKGSFTGAIAD
                     KIGQFEVANGGTLFLDEIGNLPYDVQVNLLRAIQERKIKRIGSSKEIPVDVRIIVATN
                     EDLLEMVNRGTFREDLYHRLNEFTIRVAPLAERKDDLMVYAQWFLNNANREMGKSIHD
                     FSPEAIDAMLQYSWPGNLRELKNAIRRAVLLEQSSSITVESLPTEIVSPSQKSSSTKT
                     DNLKNTKEKAEQELIIATLEKVKYNKTKAAKLLNIDRKTLYNKLKQYGIDTD"
     gene            11407..12522
                     /locus_tag="PLW22_04650"
     CDS             11407..12522
                     /locus_tag="PLW22_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOU74028.1"
                     /translation="MNENAFDFNKLIADSKNTLLNPKGYFESMSTAGGMVEPLLKALI
                     YGVVAAIINAIWFFALGSLVTLGAFGGTFGIVAFIFTIIAAIIGLFIAAVIILLLSSI
                     SSGNTDFEANLRVSAALMVLMPINSLLAVFGFISGLLGTIVSIAIGLYGLYLLYLALT
                     KTLKGKEETARIIIYVLIALLVIGQIISYFSRRALQNFGKDLSRYEQAYGEKEEDQNA
                     DYNYYSNEKPDEFPSKALEVVKVHLSTGSGSITKEKIERLVKLTEELDKYESDNAKMM
                     ELVKEFGYVDISEYTNDYLVVISGITAVSSLNAMEQLMKASEKEKKAAEGFMVDEMLK
                     NAAIQSILSGKLTESDLVTVFNNWDTVKDLENKTVTE"
     gene            complement(12512..15586)
                     /locus_tag="PLW22_04655"
     CDS             complement(12512..15586)
                     /locus_tag="PLW22_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU74029.1"
                     /translation="MDKNIAIGLIDTLADLTRKDWDIEQCRSIIQSFFSSPDFDVNGE
                     ELLKQFDSIISSESFVSGNLKHDAGLPCETMTTEQRIELAIAFYHVLSSYPESGHQKL
                     FTAWNVIGIQPEDAICLYHFLSFGPSANQFQLPGNSFISQDVLEGWEQNNSNRMIFLY
                     DENSPTQELDKALCEKRTGLGGNLLFAYFHSIGCYIVKYHGDRQVLIEDEPLIAGRSY
                     CFKPGSSITLSNGEQIRYDDITRIRLLGSGVQPVKLVVQDLEFTFPGSNQGIKPFSTV
                     EESGRVVGILGGSGVGKSTLLNLLSGKLKPHRGKVLINGYDVHNESHKLMGVVGFVPQ
                     DDLLIDNLSVYQNMLFNARLCFGNYSRKQVRELVDSTLKSLDLWEIRNLKVGNALDKV
                     ISGGQRKRLNIGLELLREPAVLFLDEPTSGLSSNDSVMVLNLLRNLADAGKLVVVNIH
                     QPSERLFRMFDRLWVLDKGGYPVYVGNPTEAVGYFREKSARVGNLVTSAVQRKRVNPE
                     DILDIIEQRQVDRSGSFTVNRKVSPEEWYRLYRESIQPKVIEIEGKTALPQGKFRLPD
                     VIKQLNVFTKRNLLSKLANRQYMLLNILEPVVLGLILSFFIKYSPGNDYIFAANRNIP
                     AFIFMSVIVSLFLGLSVSAEEIIGDRRILERESFLNLSRFSYINSKVLYLFGLSAIQM
                     LLFVGVSLTIIGVKGLTLKYWLVLFSSACFANMLGLIISSVMKSVVAIYITIPLLLVP
                     QILLSGTVVDFDNLNSSLTRRVYVPVIGDVMTSRWAYEALAVTQYVDNRYEKNFFDAE
                     MQLSRAAFRASFLIPRLQVKLEECVRLDDLDEDRSADISRHLRFIANEIEHLAQSDDV
                     PQFELLVDLKKGQLYDQLAFELNGYLLYIKKLFQEQQRAATAKRDSVYLVLKGKLGDD
                     GVYKLKQGNHNTALAEWVLRSNEVTKYLETDNRLIQKTEPIYMLPDHPFGRAQLYAPY
                     KYFNGQYIKTIWFNIIAIWLFTIALYTLLNLINNKKTGRVPVLWRVIGKFTR"
     gene            complement(15706..16518)
                     /locus_tag="PLW22_04660"
     CDS             complement(15706..16518)
                     /locus_tag="PLW22_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74030.1"
                     /translation="MKNYTNTLRVLTLAMLATVFFSCGNKQAKQAEELQAESKEQAFQ
                     EVTSYPIPTSFEVIQMINKAGASFIIGICNPVDNVGKYMTEKAKAVNLGIYGADLAYS
                     TTYQMKQETMNFLKVCKQLVEELNINAGFTPELALNVEKNIDNKDSLIVIFTNSYYET
                     YRTLVQNQKESLSLLVVAGSWIEGVYITSQIALTSRDNTEFIKILANQKAPLGKLMEL
                     MTPRVDIPEVKELMDMLAPIQAIYNKVEGEKLTNDQFQKLMEEITKVRNALV"
     gene            complement(16758..18698)
                     /gene="dnaG"
                     /locus_tag="PLW22_04665"
     CDS             complement(16758..18698)
                     /gene="dnaG"
                     /locus_tag="PLW22_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOU74031.1"
                     /translation="MIDQHTVDKILAAADIVEVVKEYVTLRKRGVNYLGLCPFHNEKT
                     PSFTVSQAKGIFKCFGCGKGGNVVNFIMEHERLNYVEALKFLAKKYHIEIEEKEVTPE
                     EIEKRNERESLMVINSYAQRYFSDMLHKHSDGKTIAMAYFRERGFKDHIIERFQLGYC
                     LDQRDAFTQSALRDGFKLDYLVKTGLTIKRDDGSTFDRFAGRVMFPIHSISGRVIGFG
                     GRILKTDKKTAKYLNSPESEVYHKSEVLYGIFHAKKAITQENKCYLVEGYTDVISLHQ
                     TGIENVVASSGTSLTQDQIKLIKRFTPNVTILYDGDAAGIKASLRGIDMILEEGLNVK
                     VVLMPEGEDPDSFARSHNASEVLEYINQNETDFIKFKTRLLLDDAKSDPIKRANLITD
                     IVRSISAIPDQVIRAVYIKECAKLMDIGEDVLYSEVGKLRRSRMEQLLQRERTEAVRE
                     HETPKIPAFVQGISCEEQEKEIVRFLLNSGNQVLFSQDVKETNELITITAAQYIIDEI
                     LNDDLEFQNLIYKKVFDEYVRLLETNVEVDNRFFINHTDPEISQMAVDLLTEKYVLSR
                     IWEKHKAEVTESPDFLQTAIPKAIMVYKSKVLKLAISKLTDELSKLKPDQVEETNQLL
                     LRLRELYALMNKMSKDLDRVIL"
     gene            complement(19218..20606)
                     /gene="lpdA"
                     /locus_tag="PLW22_04670"
     CDS             complement(19218..20606)
                     /gene="lpdA"
                     /locus_tag="PLW22_04670"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOU74032.1"
                     /translation="MNFDLIVIGSGPGGYVAAIRASQLGMKVAVVEKENLGGICLNWG
                     CIPTKALLKSAQVFEYASHAADYGVLAADVKPDFEKMIARSRGVADAMSKGIQFLFKK
                     NNITVINGFGKLKDNHTVTVTASDSTQADYTAKHIILATGARSRELPNLKQDGVKVIG
                     YRQALTLPKQPASMVVVGSGAIGSEFAYFYNAIGTKVTLVEYMPNIVPLEDEEVSKTL
                     ERAFKKAGITVKTEASVEAVDTSGELCKVTIQTKKGPEVVEAEVVLSAVGITPNLEGI
                     GIEELGIILDKGKVKVDEFYRTNVEGIYAIGDIVPGPALAHVASAEGIVCVEKIAGLN
                     PHPINYSNIPGCTYTTPEVSSVGMSEKAARDAGYEIKVGKFPFTASGKATAAGNRDGF
                     VKLIFDAKYGELLGAHMVGGNVTEMIAEMVVARNLETTGHELIKSIHPHPTMSEAIME
                     AAAAAYGEAIHI"
     gene            20839..21285
                     /locus_tag="PLW22_04675"
     CDS             20839..21285
                     /locus_tag="PLW22_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOU74033.1"
                     /translation="MRDKISIALSSFQGSANCAQSVLLAFATDEGLTTEQCLKLGAGL
                     GGGIGHKQHVCGAINAGAMIIGLRFGNSNLNDSDSKQKATTLVAEYMDKCAKRLGSTD
                     CCRILGIDLNNPEAKQKARDAGLFDMVCGNAIKVVCQLLEEDYRKV"
     gene            complement(21290..22126)
                     /locus_tag="PLW22_04680"
     CDS             complement(21290..22126)
                     /locus_tag="PLW22_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOU74034.1"
                     /translation="MIEAVKQFFQYAFIQNAIISAFLLSVITGFIGSYIVVRRLVFLS
                     GGITHASFGGIGIAWYFGLNPILGATVFSVFSALGLEYLSQGRTLREDSAIGMLWSFG
                     MAVGILFVFLTPGYAPNLMSFLFGSILTISQTDILLLLILATLTLVFFVAFYYPILFV
                     AFDSDFAKTQNVPVKFISYSLAVLIALSIVFAIRAVGIILIISLLTIPANIANIVTHR
                     FRRIIVLSALISLFSIVAGIAISFRLNVPSGATIIITMMAFYHIAKGIQLVRQRIFTL
                     KV"
     gene            complement(22138..22920)
                     /locus_tag="PLW22_04685"
     CDS             complement(22138..22920)
                     /locus_tag="PLW22_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU74035.1"
                     /translation="MTNQTDKLIELVNVTAGYGKNIVLRNVNLAINTNDFIGIIGPNG
                     GGKTTLIKLLIGQLKPFTGEVIYHTKGNGSLIGYLPQASQIDKNFPISVREVVLSGLL
                     EKKGIFGGYSRQQKGVALDLMKQVGIANLQNKSIGQLSGGQLQRALLCRALISAPQVL
                     ILDEPNTYVDSNFEGELYEMLRKLNERMAIVMVSHDLGTITSYVKTIACVNGTLFYHQ
                     SNQITNEQLAAYNCPIQIIAHGPVPHTVLLDHGKENQHNHNH"
     gene            complement(22917..23804)
                     /locus_tag="PLW22_04690"
     CDS             complement(22917..23804)
                     /locus_tag="PLW22_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOU74036.1"
                     /translation="MVSRYITIATLSVLTLLTGCDIQTTDTSKPTVVVSILPLKYFVE
                     KIADSSLQVKVLVPPGSSPEMFEPTPQQMAELTQAKGYFSIGLLDFEKGMEQKLKDNA
                     EFRYIDLSQGLELIKGECEHNHNHESHQGHNHAVDPHTWMSAANARVMAKSIAQSLTA
                     LFPERESEFNANLNRLVAHIDSVDNEVRGILAGSGRKTFVIYHPALGYFSRDYGLTQL
                     SVEHEGKSPSAKGVMELVERCRAEGITTILSQSQFDTHNSQTIANELGGTVVKVDPLQ
                     ENWAESMIFIANAIAGNVK"
BASE COUNT         7109 a         5239 c         5005 g         7096 t
ORIGIN      
        1 tagtagtaat cctacctgtt ggcaaagagc cattttggat agggtaagcc cgttctgcgc
       61 tttcagcagg tttagctcag cctcgttttt ctttaccttt accttaagca ggtcattctt
      121 tatgataatg ccctcagcat agtagttctc caggtcggta ttgagtttat cgagcagggc
      181 aatgtaagcc tgaaccagct taaccttttc agccactgct gcaactcgcc agtacgattc
      241 ctctgttttg tatagtacct cggtacgttc agcttcgctt tgcgcccggg ctaacttttc
      301 gccatacttt gcaatgcgat aggtttccct gatttttcca cctgtaaaga tgggctgggt
      361 gagattaaca cctgccacgt aaacattctt attgccaaac tcagccttat cctttgggag
      421 ccaggtatat cgctgaaaga gcgggttccc atcgctatcg gtatagggtt gtccggtact
      481 ggggtcaatg gcaaaggtgc tgccaaaaac cgatgggtta aagtttgggt ttggggttcc
      541 atcggggagg gttgggctaa ggattgcgct gttaagcccg ccggttaccg ggtcaatggc
      601 cgtgtagggt ataaccggaa gcatcaaatc cttatcgagc aggctgaact ttttgttggt
      661 tcgggtgtaa agcccgtttg cgctgatact tggcaggaat tgtgtttgtg cagaacgctt
      721 caatgcacct gccgcttcaa catgctcatc ggcaattaaa accttttggt tatgctccag
      781 cgccatttgc cggcactgtt ccagagttag aacttgctga cccgaaacat agccaatacc
      841 aattagccat gccccgaaaa aagaaaccaa tcttaaaatc ttcatagctg ttgtatgtta
      901 acgttttaca attccgtaaa aaagaatatc gagcatatcg tcaataacca tattaaggct
      961 ctccacaccc atggttgagg taaccagtgg aatctccaat cctttcatgg ccgtaactat
     1021 ggccaatgcc gataggttta aatcctttac cttgagctca ttcctattta taccctcctc
     1081 aagcatctgt tttacaatgg ttatctcctc gtcgtcgtac tttttcctga acttttcggt
     1141 tagctccagg ttcattaggt cgtcgttctt aaggatttcc atgtagtttg atagctgctt
     1201 gattaaatcc attcgcatct taacgtagga gcgaagcttt tcggtaacgg gcagcccact
     1261 ctcaagtatc tccttaacct taaccctaag caggttggct tcccggtcaa ccactgcctg
     1321 aaaaatctcc tccttaccct taaagtagta gtaaatggag cttttaccca ttcgtagtgc
     1381 ccgggcaata tcctccatcg atgttttctt aaagccatac cggctgaaaa cttcggtagc
     1441 cgcgctaaca atattctcgc gcattccctt gtggctatct tctaactggt tcatatttag
     1501 agtatttaaa ctttcgaaca ttttagttta acgttccaaa aatacaacat aggcttaaac
     1561 ggtgaataaa atattcgact aatttgtgtt aaatttcgaa taatttttta agtgatagaa
     1621 tttcagagga ataacaattc gaataaaaaa ttgaaaaatc gctataaaaa tgatagtttg
     1681 cacatggctt tcgcggcaat attccaatat gcaaacgatt tcatattgat agaagtcatt
     1741 ttttattgat aactttacgg gtacttttat agctgcgaat actataacag aactaaaaat
     1801 taaaccaaaa cataaacact atggacgtta aggaaatggc taagcagcac aagatgctga
     1861 accagtggaa gtacgagtgg aaaccgcttt accgcggcta caccgataaa accttgtaca
     1921 taaacgttgg aaccaacgag gttaaggaaa aacccgtacc cccaatcatg aaggagaaat
     1981 tcattggcgg taagggttac gacctgcgcc ttttgtggga tgcaaccaaa cccaatacca
     2041 aatggaacga ccccgagaac gagattgtta tctcttcggg tcccattggc ggtatcaccc
     2101 agtactctgg tactggtaaa tcgatagttt gtaccatctc gccccaaacc gatattgtta
     2161 tcgactcaaa cgtaggtggt ttctttggtc ctttcctcaa gttctcaggt tttgatgccc
     2221 ttgagattca gggaaaggct aaaaacgatg tgatcattta catcgacggc gttgagcata
     2281 aggttgagat ttttgaagct cccgccgagc cgctcgacag ccatattctt ggcgaggtgc
     2341 taagcgagat gtttgccaac gacgaagccg ataagaagaa tatcggtata gtatcaactg
     2401 gcgctgctgc cgaccatagc cttattggca tgctaaactt caccttctac gatcccaaac
     2461 gtaagtgcat ccgcctaaag caagccggcc gtggtggtat tggtaccgtt ttccgcgata
     2521 agaagattaa ggctattgtt tgcaagattc ccggcgttaa gggtaacctt aacaacgtgg
     2581 ccgaccttga agccattgct gagcgtggcc gccgctttaa ccgcgaaata cgtgagctcg
     2641 acgataagca gtgccagatg cgcagcaagg gtaccgccca cctggttgaa atcatgaacg
     2701 attacgacct gctcccaacc cacaacttcc agtacggctc gcataaggat gctcacaaga
     2761 ttcattccga tgtatggaag agctacttca cccagggtgt tcccgatggt tgctggattg
     2821 gttgcaacat gagctgcgcc aagggtgtgg ataagttcga gattaaaacc ggcccctaca
     2881 agggacagaa agtgattgtt gatggtcccg agtacgaaac cgctgccgga ttaggctcca
     2941 actgcggaat ttttgatcct gaatacgtta ttgaggccaa cttctactgc gatacctacg
     3001 gcatttgcac catcacctgg ggcaccacca tggcattcat catggagtgc tatgagcgcg
     3061 gtattcttaa taaggagcgt accggtggtc tggaactcag gtttggcaac aaggatgctg
     3121 cccttgagct gctgcaccag gttgcccgag gcgaaggctt tggtaaactt gccgggatgg
     3181 gcgttaaaaa gcttaaggaa cactttgtta aggagtacgg tgccgatgag aaattcctga
     3241 acgacattgc catggttaac aaaggcctgg agtacagcca gtacgtttca aaggaatcgt
     3301 tggcacagca gggtggctac gccatgacca acaagggccc acaacacgac gaggcttggc
     3361 tcattttcat ggatatggtt aacaaccaca taccaacctt tgaggataag gccgaggcgc
     3421 tgcactactt ccccatgttc cgtacatggt ttgggcttca gggtctttgc aagctcccct
     3481 ggaacgatgt tgagcccgaa aacaacgccg agaccgatga gccagctaag gttcccgagc
     3541 acgttgacaa ctacgttacc atatacaacg ccgtaactgg cagcaatatc gataagcatg
     3601 agcttatccg ccagagcgaa cgcgtttaca acttccagcg catcttcaac attcgccggg
     3661 gctatggtac ccgcaagtac gacgctcaac cctaccgcgc tgctggtcct gttaccaagg
     3721 aggagtacct cagccgcgag gagcgctacg ataagcagct taaggagcag gtgggagttg
     3781 atcctaccaa gatgaccctt gaggagaaga tggccgccct acgcaagtac cgcgaggatc
     3841 gctacgagaa actgctcgat gccgtttacg agcgccgtgg ctggaataag aatggcgtgc
     3901 ccaccattga gcacctcaag aaaattggca tggatttacc cgagctcatc gaagtagtaa
     3961 aaccattgca gtaatacact cacctaacca aacctaacca aatcccccgg tacctaaaca
     4021 gtccggggga ttttatttag tgtgttttaa cgcagagcta tgaagaaaaa gtcaaggagt
     4081 aagggaggga tttaacggct ggggggggtg accagtttgg aggtgcgggg cttaagatat
     4141 atcctacgtt aagaagctcc tgcgtgccaa cccgcttgcc taatccattt aacccaaaat
     4201 ggttacatcc cttgttgaac cctagcgttt tttcttaaag tttaaattta acctgtttac
     4261 tttatcacaa attatttcaa gtgtcgttta gttaaggata tttatcaagt tgagcgatgc
     4321 gaagcatctc aaaacaataa aaatagaaaa ccctcgattc actaagaata ataaaaggag
     4381 tatccggttt gttaactata ctatattgat tattaatcct taaagaaata gatttctttc
     4441 aaggatttaa cactcctatc tgcaaaaaag tcagttggca agtatattct ataatatcca
     4501 ccatcttcaa cattctctgg aatagaaagg ataggaatta cttggtcgct tctattaaac
     4561 aactcactat agctataaat tgctctgtat ccgtcaattg atgaaaagca aactaatcca
     4621 ttcattatcc attccctatc aaataaattg ttaggttttt tcataagcga cttcaaaggt
     4681 attcccttaa attcgggatt ggggtgatag cccatcccca taccaacaaa tattgaagtg
     4741 tatttaataa ttcttgaagt atctgaaatt gttggaatag aaaaatcgaa accattgtca
     4801 aatacaacat ttacagctgg gctatacata ggtttaattt ctctgttgat tggatattct
     4861 tttttgtcga aagatttgat tgtaatagaa gttgggtttt ctattgttct ataagaaaaa
     4921 agatcatttg ctgctacaac cttccaacaa ttgcccgtga ggtagtttac ttctttttta
     4981 tgtggcttta tttgagccac ttcagtggca ataataatct gatgtgggtt aatagtatga
     5041 aaaatttctg accaactgaa cgaaacgtgc tcccctttat catttgaaat aacaacatac
     5101 acatcaatcg aaggtctaaa aaacttttca ttctttttgt caagattata agagttcaaa
     5161 aggtcaaaaa gagaataccc aatgtaacga aaagcaccaa taaatttagg atcattgctt
     5221 tcgtcaagaa tagcttcttt gacaaaaact tcccgcttgt agaattttct taaatcaacc
     5281 actcctgttt ttttcacctc cccctgaatg ctaatctctg aaatattcag tttttttgca
     5341 tcagtttggt ggaataaact attaccattc tgggataatg taatggatgc acccgataat
     5401 gcatcaatcc aagttcttgg caaatagcta caagcagcag aaatcgtaat aaacccgagt
     5461 gcacttaaaa tttttgtaat attttttctc atataccaat cttttgttat ttttgcgtta
     5521 tgcaaatata tgcatatcgc aaaagtgcaa aaaaaattcc agaagcaaat gatacatact
     5581 gaaaaaattt ttttcgctta gggttgccta ttaacattag tgttgcttca attttaagtc
     5641 agatattcag ttgtttaaat ttcacctttt aaaaacaatt ttaaatgaaa aagacctttt
     5701 tattttttgc aatggcgttt tttagcctaa acaccttttc gcaggttagc ctcagcggaa
     5761 aagtaattga taaggaaacg ggaaatggaa tcccatttgc aattgttgaa gtaaaggatt
     5821 ctgatatcaa aacagtatca cagagtaacg gggcattttt actccaaatt acaggtaaca
     5881 atacaattga aattacagct aaaagtttag gatactcatc caagtcactt gttataagcc
     5941 ccgattcatt taataaagaa attacaataa tgcttgatgc tgaaatatat gagttaaaaa
     6001 ccgtagttgt aacagcaacc cgaagcgaga gaaaaatttt agatgtaccc caaagggtcg
     6061 aggttattgg taacaacaag attcagggaa tccctgctct ttcggctgat aattacttgt
     6121 actccatccc aggcgtttcc ataagtagag gtgcatcaat ttttggagca gctgatattt
     6181 cgttgcgtgg tatgggcaac gaagcaggtc gaacattggt aatgattgat ggtgcaccca
     6241 taaataagaa tgatggggga acagttaact ggaattctat taatacggct aatttaaaac
     6301 aaatagaagt tttaaaaggc tctgggtcaa caatacatgg tggaaatgca atgggcggag
     6361 ttataaattt aattactcct acgccaagcg aaccgctaca gggtgacgtt tcactaagtt
     6421 atggtagctt ttcaacactt caaacacaag ctaacgtttc aggtataaaa gatagattat
     6481 actggtcaat aaatggaagt cacaggaaaa gcgatggata tattactagc caagcggatg
     6541 aggttgatga gttttcaatt ccttcattct taactgagta caatgttgga gcaaaagttg
     6601 gatttcaact ttctgcaaaa cataaaattg aatgttcagg tgggtactat tcagggcaac
     6661 gtggtactgg ttcaaacttc acaggttatg gttttacaaa tgaaaattta gcctctagca
     6721 aaggtgctta taactggtac actgacataa acagcagggt ttcttatcag ggtatattta
     6781 atgacaacag caacctaaag gttaattttt atactcaacg tgagtactat aagaacataa
     6841 gagaaagttt aaagaataat gaaataaaaa gatatgacgt gctttcaact cgtgatgaca
     6901 ttggcttact ttctggttac agtttttcat tgggcaaata caacaaatta acttcaggta
     6961 ttgatatgcg ttatggtgct gttgatggtg ccgacactta cattacatct accgataaag
     7021 ttttaaactt agggaaaatg aatttagtag gtgtttacct gcttgatgag ataaaaatcg
     7081 gagaaacacc gcttagcata ttagctggca ttagatatga ttacgcaatg ttctacgatg
     7141 gtaaattttt agttgaaaat ccaacaagtg aaactgcttt cttacagaat ttctcgggag
     7201 atttgaacaa tgcaaatttt tctgctttta gtccaagggt ctcatttcag tattaccaac
     7261 cccaaaaatt cagaacctac cttggctata gcagaggtta cagagctcct gtacttgatg
     7321 atttgtgccg tacaggtcgt atttcaggcg gaatgaaaat tgctaatcct ttgctaaaac
     7381 ctgaatatat agataactac gaaattggtg cagatatttt gtattttaaa aaggtaagtt
     7441 tttcaacatc atttttctac tctattgggc aagattatca cgcatatatt gcaactggcg
     7501 attcaattat tttgaataat aagcttcgcc caattatgac taaaagcaat atcggaaaag
     7561 ttgaaattta tggagcagaa tttaatgtaa atttcacgtt aagcaatagt tttatttggt
     7621 ctttagcata cagtcatatt gttactaata ttctagatta taagagactt aactctgaaa
     7681 ttgaagataa cttagttggg aaagaactag tttatcaacc aagtgatata ttcaatacga
     7741 ctttaatttg gaaaaattct ataatcaatt catcagttgt tttcaactat aaaggagcgc
     7801 aatggattaa tgatattaat acagaaaaaa ttaatagtta ttatttcatt gacttacagc
     7861 tatggaggcc tatatataaa ggcttgcaag cttcactaat gattcataat ttacttaata
     7921 atcattatat tgattcacga aatatgattt cacctggtcg tatgctgtca ttgcaaataa
     7981 aatatgaatt ctaggtgcgt taaaatgaat gataaagtca agtttttctt gcttttaatc
     8041 aaataggttt aagtattaac gtttacaaga cgatttattc gtcgtttaat actcttgtgt
     8101 ccaacggttg gggtgtgacc agtttggggg tacggggctt gagatatatt ccactttaag
     8161 gagttcctgc gtgccaaccc gcttgcctat ccattcaacc caaaatggtt acatcccttg
     8221 ttgggctcca ttattatttt ctattattaa ttcaatctgt tcttctatta gccattattc
     8281 gtatccaatc catttttgaa taataatacc ttttttgtta ataaggcaaa atgtaggtat
     8341 tccctttatc ccgtaattct cccaagtatt actatacatt cctcttccgt tacataatgt
     8401 tagccaagga attttttcct tttcaacatg ctttaaccag tcatatacac ttacgtcaga
     8461 gtaaaatcaa ctatttcaag tgatttatga tatttttcat atacccttct gagttcaggt
     8521 atagatttta cgcaagcacc acaaccagat cctgtaaatt tcaatagaat atatttccct
     8581 ttaatatctg aaatcttcat aaattcgcca ttactattta ctccactaaa atcatgatat
     8641 ttatcgccaa tttcaggggg tagcaaattt tgtaattgtt ctacagaatt gtgctgcttt
     8701 gctatttctt tttcaattat taatgatttt tcaattattc tattatttat actaatctat
     8761 tttatgcttt ttttaatcct ttatcattac ctgaaatagt attcgtgttg catctatatt
     8821 catggttttt acatgataaa atgatataat tataaacaat agggtgaagt aaatatgctt
     8881 attcatttta ataaaatcgt tttatagtcg aaatatgaac taaagtaatt tttatattct
     8941 aaaatcctgt taaccttcag agcaatcttc tgaaaacttt caaaaaattt atcgatatct
     9001 cttactataa acctaaattc attgcatttc tttaattaca acggtcggtg gtttgggctg
     9061 ctggggagtt aagagccagc ggcgtcatcc cacgttaaaa cgaaactaaa ttactaaaaa
     9121 ctttcgtatc tgcatcatca gccctagtga aaggaactgc ctgttagcgc aagtaattta
     9181 accatttctc ttttctaaat ccaatttcaa ttcttcaaat ttttctttaa tttttttaag
     9241 ttcgttgtat tggatgtttt ttaaatttag tactttctca gtcgatggta attctatttt
     9301 tatttcgtaa agttggatag ttacttttct aatatcttca aattttattt tttctatcgt
     9361 tttatttttt gatagcaagt atctcatttc tttttcatcc cactcgatga ataatttttc
     9421 attcttttta ttagaatacc caagagcaaa taaagctgca ccttgtataa cttgtataat
     9481 ataaaataaa tagttccagt ctccttgatt aaaatctgtt ttgtccccat tcattagcga
     9541 ctttatcaaa aaagtcattc caaaaagaat aaatagaagg ccaaaaatct taacataaat
     9601 ttctaagcct ttttgttttt tgtaaatctg aattctttcc atatcgtttc ttattatttg
     9661 ctctaactcg tttataaccg taccaaaagt aattttataa atctattttg ggtgtataac
     9721 cgtacttttt gtagggttta tttattcaaa aataaaataa tttcataaat catcaaaagg
     9781 actttttaat tttttttaga ccattttacc tggaaatgaa tttgttagta gatgattgaa
     9841 tgagtggggc tcaaattaaa ctgctttgct tgatttacat atttttttta ttaactaagc
     9901 actaaacacc atactccaaa cacttaaatt tctatttctc caaaatagtt tctaccttag
     9961 cgttggtgaa tgggataaac taaatggcta agattcttgt agttgacgac gattctacct
    10021 tttgcctgat gcttaaaacc tttctggtaa ggaacgggca taaggttact gaggccttct
    10081 cgtttgatga tgcaaggcag gctattcaga ccacaacgtt cgatgtggta ctaaccgatt
    10141 tccgcctgcc tgagaagtcg ggaatggatg tgcttaccga ggcaaaacgt aaaaaccgcg
    10201 ataccattgt aattgtgatg accggctatg gcgatatacg catggcggtt aaggccatta
    10261 agcagggtgc ctacgattac gttaccaagc ctgtaaaccc ggaggaaatc ctggcaacca
    10321 taaacaacgc tctgcccatc tcggtgggga aaacccccga aaccgatacc gatgctttca
    10381 cctttattga gggcaaaagc cgttacagcc aggaggtgat gcgttacatc aacattgttg
    10441 cacccaccaa cctatcggtt attatacagg gcgatagcgg aaccggcaag gagtttgttg
    10501 cccgtcaaat acaccgcgcc agcaaacgga acagcaaacc ctttgtagct atcgactgtg
    10561 gcgcgctacc gcgcgagctg gccgccagcg agttctttgg tcatatcaag ggttcgttta
    10621 ccggggccat tgccgacaag attggccagt ttgaggtggc caatggcggt actctcttcc
    10681 tcgacgaaat tggaaaccta ccctacgatg ttcaggtcaa cctgctacgt gccattcagg
    10741 aacgaaaaat aaagcgcatt ggcagcagca aggagatacc cgttgacgtg cgaataattg
    10801 ttgccaccaa cgaggacctg cttgagatgg tgaaccgcgg aacattccgc gaggaccttt
    10861 accacaggct caacgagttt accatcaggg tagcgccgct agccgagcgc aaggacgatt
    10921 tgatggttta cgcccagtgg ttcctgaaca atgccaacag ggaaatggga aagagtatcc
    10981 atgatttttc gcccgaggct attgatgcca tgctgcagta cagctggccc ggtaacctca
    11041 gggagctaaa aaatgccatt cgcagggcag tgcttcttga gcagtcaagc agtattaccg
    11101 ttgaatcgct acccactgaa attgtatcgc ccagccaaaa aagcagctcc acaaaaaccg
    11161 ataaccttaa aaacaccaag gagaaggccg agcaggagct gattatcgct actttagaaa
    11221 aggttaaata caacaaaacc aaggccgcca agctgcttaa tatcgataga aagacgctct
    11281 acaataagct taagcagtac ggtattgata ccgattaaag ccctagcttg ataaatattt
    11341 gcctaaagtt gactttctcg tttttttaac agaactttcg ttcttgaacc caaaaaacta
    11401 ataactatga acgagaatgc tttcgacttc aataagctca ttgccgattc aaagaatacc
    11461 ctgcttaacc ccaaagggta ttttgagtcg atgagtaccg ctggcggtat ggtagaaccc
    11521 ctcctcaagg ctctcattta cggagtagtt gctgcaataa taaacgccat ttggttcttt
    11581 gccctgggaa gcctagtaac acttggggca tttggtggta catttggtat tgtagcgttt
    11641 atttttacaa taattgcagc cataattggg ctatttattg cagccgtaat tattcttctg
    11701 ctatcgagta taagtagtgg caataccgat tttgaggcaa acctaagggt aagcgcagca
    11761 ctaatggtgc ttatgcccat caactcttta ctcgcagtct tcgggtttat aagcggctta
    11821 ctgggaacaa tagtatcgat agccattggc ttatacggat tatatctgct ttaccttgct
    11881 cttaccaaaa cactaaaagg gaaagaggaa acagcccgca ttattattta tgttttaata
    11941 gcccttttag taattggaca aatcattagt tactttagca gaagggctct tcaaaacttt
    12001 ggtaaggatt tatcgcgtta tgaacaggct tatggcgaaa aagaggagga ccaaaacgcc
    12061 gactataact actacagcaa cgagaaaccc gatgagttcc caagcaaagc gctggaagtg
    12121 gttaaggttc acctttcaac gggtagtggt tcaataacaa aggagaagat tgaaagatta
    12181 gtaaagctta ccgaagagct cgataagtac gaaagcgata atgccaaaat gatggagctg
    12241 gtaaaggagt ttggctatgt cgatatttcc gaatacacca acgattacct tgttgtaata
    12301 tcgggcataa cagcagtatc gagcttaaat gccatggagc agctaatgaa agcctcggaa
    12361 aaggaaaaga aagcagccga aggctttatg gttgacgaga tgcttaaaaa cgcagccata
    12421 caatcaattc tgagcggtaa gctaaccgaa tcggatttgg ttacagtatt caacaactgg
    12481 gatacggtta aagacctcga gaataagact gttaccgagt aaactttcca ataactctcc
    12541 acaaaaccgg caccctgcca gtttttttat tgttaataag gttcagcagc gtgtataggg
    12601 caatggtaaa aagccaaatg gcaattatat tgaaccatat agttttgata tactgcccgt
    12661 tgaagtactt atagggtgcg tacagctgtg cgcggccaaa gggatggtca ggtaacatgt
    12721 aaatgggctc ggttttctga atcaacctat tgtcggtttc gaggtatttt gtaacctcgt
    12781 tgctccttag cacccactcg gctaaagcag tgttatggtt tccttgtttc agcttgtaaa
    12841 cgccatcgtc gccaagcttg cccttaagaa ccaggtaaac gctatcgcgt ttggcagtgg
    12901 cggcacgctg ctgctcctga aaaagctttt tgatgtaaag taggtaacca ttcagctcaa
    12961 aagcaagctg gtcgtaaagc tgaccctttt ttaggtctac cagtaactca aactgaggta
    13021 catcgtcgga ttgggcaagg tgttcaattt cattggcaat aaagcgcaaa tggcgcgata
    13081 tatcggcact cctatcttca tcaaggtcat cgagcctcac gcattcctca agtttaacct
    13141 gcaggcgggg aataagaaac gacgccctaa aagcagcacg gctaagctgc atctcggcat
    13201 caaagaaatt cttttcgtaa cggttgtcca cgtactgcgt taccgcaagc gcctcgtagg
    13261 cccatcgcga tgtcataaca tcgccaataa ccggaacgta aactctgcga gtgagcgatg
    13321 agtttaggtt atcgaaatca accaccgttc cgcttaaaag gatttgcggc acaagcagca
    13381 acggtatggt aatatatatg gcaacaaccg atttcatcac tgagctgatt atcaacccga
    13441 gcatattggc aaagcatgcc gagctgaaaa gcaccaacca gtacttgagc gttaaacctt
    13501 taactccaat aatggtaaga ctcaccccta caaacagtag catttgaatg gccgaaagac
    13561 caaacaggta aagaaccttt gagttgatgt agctgaagcg gctcaagtta agaaacgact
    13621 cacgctccag aatgcggcgg tcgccaatta tctcctcggc gctaacacta aggcccagaa
    13681 agagcgaaac tatcacgctc ataaatataa aagcgggtat gttgcggttt gccgcaaaaa
    13741 tgtaatcgtt gcctggcgag tacttaataa agaaggaaag gataagcccc agaacaacgg
    13801 gttccaggat attgagcaac atgtattgcc tgttggccag ctttgatagc aggttacgct
    13861 tagtaaatac gtttagctgc tttattacgt cgggtaaccg aaacttaccc tggggtaatg
    13921 ccgttttccc ctcaatctca atcactttgg gctgaatcga ttcacggtag agcctatacc
    13981 actcctcggg cgatacctta cggtttacag taaacgaacc gctcctatca acctgacgct
    14041 gctcaataat atcgagtata tcctcagggt taaccctttt cctttgcact gctgatgtca
    14101 ccaggttgcc aaccctagcc gatttctccc taaagtaacc aaccgcctcg gttgggttgc
    14161 ccacataaac cggatacccg cctttatcca aaacccatag cctatcgaac attctgaaaa
    14221 gcctttcgga gggttgatgt atatttacca caaccagctt gccggcatcg gcaaggttgc
    14281 ggagcaggtt aagtaccata accgaatcgt tggacgatag gccgctggtg ggctcatcga
    14341 ggaaaagcac cgcaggctcg cgtagcagct caagcccaat gtttagcctc ttgcgctgcc
    14401 ctccgcttat aaccttatcc agagcattcc ctacctttaa attcctaatt tcccataaat
    14461 ccaagctttt taaagtagaa tccacaagct ccctaacctg cttacggcta tagttgccaa
    14521 agcaaagcct tgcattaaaa agcatgttct ggtaaacgga gaggttatca ataagcaagt
    14581 cgtcctgggg tacaaagcca acaacaccca tcagcttatg cgactcgtta tgaacgtcgt
    14641 agccgttaat cagcactttg cccctatggg gcttaagctt gcccgacagg aggttcagta
    14701 gggttgattt acccaccccg ctaccgccaa gtattcccac taccctcccc gactcctcaa
    14761 cggtactaaa gggcttaatg ccctggttgc ttccgggaaa agtaaactca agatcctgaa
    14821 caaccaattt aaccggctga acgccgctac ccagcaagcg tatacgtgtt atatcgtcgt
    14881 atcggatttg ctcaccattg ctaagcgtaa tgcttgaacc cggcttaaaa cagtacgaac
    14941 gtccagctat aagtggttca tcttcaatca aaacctgtct atcgccatgg tacttaacta
    15001 tgtaacagcc tatgctatgg aagtaggcaa aaagtagatt gcctccaagt ccagtacgtt
    15061 tctcgcataa tgctttatca agttcttggg taggggaatt ttcatcgtat aggaaaatca
    15121 tcctattgct attgttttgc tcccaaccct caagaacatc ttgtgaaata aaagaattgc
    15181 ctggaagctg aaattgatta gcggatgggc caaagcttag gaaatggtac aggcaaattg
    15241 catcctctgg ctgaatgcca attacattcc atgcggtaaa aagcttttga tgcccagatt
    15301 caggatacga agagagtaca tggtaaaatg ctatggccaa ttcaatacgc tgttcggtgg
    15361 tcatcgtttc gcagggcaaa ccggcatcgt gtttaagatt accgctaacg aaactctctg
    15421 agctaatgat actatcgaac tgcttaagaa gctcctctcc attaacatca aaatcgggtg
    15481 atgaaaagaa gctttgaata atgctccggc attgctcaat atcccagtcc ttgcgagtta
    15541 aatcggctag cgtatcgata agtcctatgg caatattctt atccaaactc tttttgcaat
    15601 ttctttctaa ctaattagat agtttaaaaa gttaaaagtt acacgaaaaa aataaaaaaa
    15661 cgcacctttt tggggtgcgt tttaatgatg gtttataaaa atgaattata ccaaggcatt
    15721 acgaaccttt gtaatttctt ccattagctt ttgaaactga tcgttggtca atttttcacc
    15781 ctcaacctta ttgtaaatag cctgaatggg ggcaagcata tccatcagtt ccttaacctc
    15841 aggaatatct acacggggag tcatgagctc catcagcttg ccaagtgggg ctttttggtt
    15901 tgcaaggatt ttgataaact cagtgttgtc gcgacttgtc aaagcaatct gcgatgtgat
    15961 ataaacaccc tcaatccagc taccagcaac taccaaaagc gagaggcttt ccttttgatt
    16021 ttgaaccagg gtgcggtagg tttcatagta ggaattggta aaaataacta tcaatgaatc
    16081 cttattgtcg atattctttt caacatttaa tgccagttca ggggtaaatc cggcatttat
    16141 gttaagctcc tctacaagct gtttacaaac cttaaggaaa ttcatggttt cctgtttcat
    16201 ctggtaggtt gttgagtagg caaggtcagc accgtaaata cccaggttaa cggccttagc
    16261 cttttcggtc atgtatttcc caacattatc aaccgggtta caaatgccaa taataaaact
    16321 tgctccggcc ttattaatca tttgaattac ttcaaaagag gttggaattg gatagctggt
    16381 tacttcctga aaagcttgct cctttgactc ggcttgtagt tcttcggctt gctttgcctg
    16441 cttattgcca cagctgaaaa aaactgttgc cagcatggct aacgtaagaa ctcgtagggt
    16501 gttagtgtag tttttcatat ttataaatgt ttaaaggtta aactatttag gttcagcaaa
    16561 aaacatgaca taaacaataa ccacaattgc tttaatatgt ttgaaccagt ataaccggca
    16621 aaggtttaaa gcaaccgaaa ccctaatttg gtttttgtaa atttactaaa aacaataata
    16681 acaaaagaaa aacacttaaa aaagttagca gaatttaagg gaaggtgtca ttatgcttat
    16741 gtaaactgct aaaattgcta cagaattacc ctatctaaat ccttcgacat cttattcatt
    16801 agggcataaa gctccctgag ccgtaggagt aactgattgg tttcctcaac ctgatcgggt
    16861 ttgagcttac tcagctcatc ggttagcttg ctaatggcca gcttcagcac tttgcttttg
    16921 taaaccatta tagccttggg tatggctgtt tgtagaaaat cggggctttc agtaacctcg
    16981 gccttgtgtt tttcccaaat ccggctaagc acgtattttt cggtaagtaa atccacggcc
    17041 atttggctaa tttcaggatc ggtgtggtta ataaaaaatc ggttatcgac ctcaacgttt
    17101 gtttccagca aacgcacgta ttcatcaaaa acctttttgt aaatcaaatt ctgaaactct
    17161 aaatcatcgt taagaatctc atcaatgata tactgagcag cagtaatagt gataagttca
    17221 ttggtttcct tcacatcctg gctaaacagt acctggtttc cgctattcag caggaaccgt
    17281 acaatctcct tttcctgctc ctcgcagcta atgccttgaa caaaagcagg aattttgggt
    17341 gtttcgtgtt ccctgaccgc ctcggtgcgt tcacgctgga gcagctgctc cattctgctc
    17401 ctacgcagct tgccaacctc ggagtaaagc acatcctcgc caatatccat taacttggcg
    17461 cactccttga tgtaaaccgc ccgaatcacc tggtcgggta tggcacttat tgagcgtaca
    17521 atatcggtaa taagattagc gcgtttaatg ggatcgctct tggcatcatc gagcagaagg
    17581 cgggtcttga atttgatgaa atcggtttcg ttttggttga tgtactccag cacctccgag
    17641 gcgttatggg aacgggcaaa gctgtcgggg tcttcgccct cgggcataag caccacttta
    17701 acgttaagcc cctcctccag tatcatatcg atacccctga gcgaggcctt aatgccggcg
    17761 gcatcgccgt cgtaaagaat ggtaacgttt ggggtaaagc gctttatcag cttaatctga
    17821 tcctgcgtaa gcgatgttcc ggaactggcc accacattct caatgccagt ttggtgcaac
    17881 gatattacat cggtgtaacc ctctaccagg taacacttgt tctcctgggt gatggctttt
    17941 ttggcatgga aaatgccata aagcacctcg cttttatggt aaacctcgct ctcggggctg
    18001 ttaaggtact tagccgtttt tttatcggtt ttaagtattc gccccccaaa accaataacc
    18061 ctgccactta tgctgtggat tggaaacatc accctaccgg caaagcgatc aaaggtgctg
    18121 ccgtcgtcgc gctttatggt aagtccggtt ttaacaaggt agtcgagctt gaaaccatcg
    18181 cgaagtgcgc tttgggtaaa ggcatcgcgc tggtccaaac agtaacccag ctgaaacctc
    18241 tcaatgatat ggtccttaaa tcccctctcg cggaagtaag ccatggcaat ggttttgcca
    18301 tcggaatgct tatgcagcat atcggaaaag tagcgctggg catacgagtt gataaccatt
    18361 aggctctcgc gctcgttccg cttctctatc tcctcggggg taacctcttt ctcttcaatc
    18421 tcaatgtgat acttcttggc caggaactta agagcctcca cgtagttaag ccgctcatgc
    18481 tccataatga aattgaccac attcccaccc ttgccacagc caaagcactt gaaaataccc
    18541 tttgcctgac tcacggtgaa cgagggcgtt ttctcattat ggaatggaca aaggcctagg
    18601 taattaaccc cgcgtttccg aagggtaacg tattccttta ccacctcaac aatatcggct
    18661 gctgccagta ttttatcaac tgtatgctgg tcaatcattt agcaatacat ttttggcctt
    18721 tttcttttgt aaagatagtg taagttgatt ttatggctcg atattttcag ggtatgttta
    18781 tgaacagtta cagcaaagtg attaactaaa ggtgttaata agtgggtttt cgttgttagt
    18841 tgatttttac tcgtttttta ggtacattac ttctcacact tatttatcga tgagttaaca
    18901 accatattga ttatctttgt ggttaaatgc aataagtatg tggaatataa gtattgaaac
    18961 ggtagcatac attctaatca ttgggttaca tctgtttact ttttttgatg tgctgaaaaa
    19021 cctgaaaaag cagaggactc ccataattgt tgttttggca tttttcccgc caatactagg
    19081 ccctgtaatc tattttttga ttaagagttg gaaacccagg cagccacgga cattcatgga
    19141 gaaaaagcgt agattttcat aaaaacaaaa aaccgccacg ggaaacgtag cggttttgtg
    19201 gtaatatcaa agtaaattta gatatgtatt gcttcgccgt aagcagcagc agcggcttcc
    19261 ataattgcct cgctcatggt tgggtgcgga tgtatgctct tgattagctc atgaccagtg
    19321 gtttcgaggt tgcgtgccac aaccatttcg gctatcattt cggttacatt gccacccacc
    19381 atgtgggcac caagcaactc gccatacttg gcatcaaata tgagcttcac aaagccatcg
    19441 cggttaccgg ctgcagttgc cttacccgag gctgtaaaag gaaacttgcc caccttaatc
    19501 tcgtaaccag catcgcgggc tgctttctcg ctcataccta cgctcgaaac ctcaggcgtg
    19561 gtgtaggtgc aaccgggaat gttgctgtag tttattggat gggggttaag cccggcaatc
    19621 ttttcaacgc aaacaatacc ctcggcggaa gcaacgtggg ctaatgcagg acccggcact
    19681 atatcgccaa tggcgtaaat gccctcaacg ttggtgcggt agaactcgtc aaccttaacc
    19741 ttgcccttgt ccagaataat acccaactcc tcaataccta ttccctcaag gttgggcgta
    19801 atgcctacgg ccgaaagcac aacctcggcc tcaaccacct cagggccctt tttggtttgg
    19861 atggtaacct tacatagttc gcccgatgta tcgaccgcct caaccgatgc ttcggtttta
    19921 actgttatgc cagccttttt gaaggcgcgc tcaagggttt tacttacctc ctcgtcctcc
    19981 aggggaacaa tattgggcat atactcaacc aaagttacct tggtgccaat tgcgttgtaa
    20041 aagtaagcaa actcactgcc aatagcacct gaaccaacaa ccaccatgga ggccggttgt
    20101 ttgggtaaag tgagagcctg acggtagcca attaccttca ccccatcctg ttttaggttt
    20161 ggtagctcgc gcgaacgcgc acccgtggca agtatgatgt gttttgcagt gtagtcggct
    20221 tgtgtactat cggaagcggt tactgttacg gtgtggttat cctttagctt accaaaacca
    20281 ttaataacag tgatgttatt ctttttgaaa aggaattgta ttcccttgct catggcatcg
    20341 gccacaccgc ggctacgagc aatcattttt tcaaaatcgg gttttacatc ggctgccaac
    20401 actccataat cggcagcatg cgatgcatac tcaaaaacct gtgcgctctt aagtaacgct
    20461 ttggtgggga tacagcccca gttcaggcat atccctccaa ggttttcttt ttccaccaca
    20521 gccactttca tgcctagctg gcttgctcgt attgcggcca cataacctcc ggggccgctt
    20581 ccaattacta taaggtcgaa gttcatagag taaaattttt gtttgtcaaa attacaataa
    20641 caaatggaat gggaaaatgt tagatgtcat acatcgcatt aatagttcac taatatcatt
    20701 gttcatataa tatttgggca ttatcagatt gatttcgatt aatacaagtg ctgctaatca
    20761 atataaacat gatgagttat aaaaaaacag aaacaatttg ggaaacctat tcgtaaatga
    20821 actaaaactg cagattttat gcgagataaa atatccattg ccctatcgag ttttcagggg
    20881 agcgccaatt gcgcacagtc tgtattgctt gcatttgcca ctgacgaggg gttaactacc
    20941 gagcaatgct tgaagctagg tgctggactg ggtgggggta ttggtcataa gcaacatgtg
    21001 tgcggagcaa ttaacgcagg ggcaatgatt atcggattaa ggtttggcaa tagtaacctg
    21061 aacgattccg actccaagca aaaagcaacc acacttgtgg ccgagtacat ggataaatgt
    21121 gccaaacgat tagggtcaac cgattgctgc agaatactag gaatagattt gaacaacccc
    21181 gaagcaaaac aaaaggcccg cgatgcaggc ctgttcgata tggtgtgtgg caatgccatt
    21241 aaggttgttt gccagctgct ggaggaggat taccgtaagg tttaacaatt tacactttca
    21301 gtgtaaaaat ccgctgcctt accaattgaa tcccttttgc aatgtggtag aaagccatca
    21361 tggtaataat gattgttgca cccgagggaa cattcaacct gaacgagata gctatacccg
    21421 caacaatcga aaaaaggctg attaaggccg ataacacaat tatccttcta aaccgatggg
    21481 ttactatatt ggctatgttt gcaggtatgg ttagcagcga tatgattaga atgatgccca
    21541 cagctcttat ggcaaaaact attgagagag caatgagaac agccaacgag tagctaatga
    21601 acttaaccgg gacattctgt gttttggcaa aatcgctatc gaatgccaca aaaagaattg
    21661 ggtagtaaaa agccacaaaa aatactagcg taagcgtggc aagtatcagc agcagtaata
    21721 tatcggtttg ggaaatggtt agtatgcttc cgaacaggaa cgacattagg tttggagcat
    21781 aacccggcgt taggaaaaca aaaagaatac ctacagccat gccaaacgac caaagcatcc
    21841 ctattgccga atcctcacgc agcgttcgcc cctgcgataa gtactcaagc cccagtgccg
    21901 aaaatactga aaaaacagta gcccctagga tggggtttag cccaaaatac caagctatac
    21961 caataccacc aaaggaggca tgggtaatgc caccgctcag aaaaaccaat cgcctaacca
    22021 caatgtacga accaatgaac ccggttatta ccgaaagcag gaatgctgaa attattgcat
    22081 tctgaataaa tgcatactga aagaactgtt taacggcctc aatcataaca gggataatta
    22141 gtgattgtga ttatgttgat tttctttacc atgatcgagc agaacggtat ggggaaccgg
    22201 accatgagca ataatttgga ttgggcaatt ataggctgca agctgctcgt tggtaatctg
    22261 gttcgattgg tggtaaaaga gtgtaccgtt aacgcaagca atggttttaa catacgaggt
    22321 tatagtaccc aaatcatgcg ataccattac aatagccatt cgctcgttta gcttgcgaag
    22381 catctcgtaa agctccccct caaagttgct atccacgtat gtgttgggct catccaggat
    22441 aagcacctgt ggggccgata tcaatgccct gcaaagcaat gccctttgca gctgaccacc
    22501 cgagagctgc cctatacttt tattctgaag gttcgcaatt cctacctgtt tcatcaggtc
    22561 aagggcaaca cccttttgtt ggcggctgta gccgccaaaa atcccctttt tctccaaaag
    22621 ccccgatagc actacttccc ttacggatat gggaaaattt ttatcaatct ggcttgcctg
    22681 tggcaggtag ccaattagtg aaccgtttcc ctttgtgtgg taaataacct ctccggtaaa
    22741 gggttttagc tgacctatta gtagttttat tagggttgtt ttccctccac cattcgggcc
    22801 aattatacca ataaaatcgt tagtgttgat ggcgaggtta acattacgca aaacaatgtt
    22861 tttaccgtat ccagctgtaa cgttaaccag ctctatcagc ttgtcggttt ggtttgtcat
    22921 tttacattac ctgcaattgc gttagcaata aaaatcatac tctcagccca gttttcctga
    22981 agcggatcaa ccttaaccac tgtaccgcca agctcattgg caatggtttg agagttgtgc
    23041 gtatcgaact ggctctgcga tagaatggtt gtgataccct cggcacggca gcgctcaacc
    23101 agctccatca cccctttggc cgatgggctt ttcccttcgt gctcaacgct tagctgggtt
    23161 aacccgtaat cgcgggaaaa gtaacctaaa gctggatggt atattacaaa ggttttacgc
    23221 ccggaacctg ccagaatacc cctaacctca ttatcaaccg aatcgatatg ggcaaccaag
    23281 cggttgaggt tggcgttgaa ctcgctttcc ctctcgggga aaagtgcggt taggctttgg
    23341 gctatggatt tagccattac ccttgcattg gcagccgaca tccaggtatg ggggtcaacg
    23401 gcatggttat gcccctgatg gctttcgtga ttgtggttgt gctcacattc acccttaatc
    23461 agctcaaggc cctgcgacag gtcgatatac ctgaattctg cattatcctt tagtttttgc
    23521 tccattccct tctcaaaatc gagtagccct atggaaaagt agcctttagc ctgtgtaagc
    23581 tcagccattt gctgaggggt tggctcaaac atctcagggc tgctgccagg aggaacaagt
    23641 actttaacct gaagggagga atcggcaatc ttttcaacaa agtattttag tggcagtatg
    23701 cttaccacta ctgttggctt actggtgtct gtagtttgga tatcacaacc agtaagtaat
    23761 gtcagtactg aaagcgttgc aatagttatg tatcgagaaa ccatagcaaa tattagtttg
    23821 atgataaagt tacacaatta tagtagcatt aagtaaccac aatcattgga atttgtttaa
    23881 aatgtttttc taattagcga atctgctcta aatcaattaa attaaaagat accatgttaa
    23941 atattgccaa ttttaaatgt taaacaatcg ctactaataa actgaatagt aaaaagcaaa
    24001 aaccaacaca aaactttact ccaaattcgt catctgatgc aagttttaag aacatttttt
    24061 actctattat tttttgtcta ttccaacaaa ctcagaatta ttacaaatca tacccgctaa
    24121 aatcacgcgc ttaacggctc gcgtttaacg atttaactcg taatagagat ttctcgacga
    24181 gctcggaatg acaataggta ataattagat tccgtttaac ggctcacgat taacgattca
    24241 tgcacttctt ttaacttcct ctaacttcct gacattctct aaaaaactcg tttaacggct
    24301 cacggctcac gtttaacgtt ttagctcaat tgagattcct cgacatagtc tatcccgata
    24361 tatcgggact tggaatgaca ataggtaata atttttgacc cgtcctgaaa aatgtctaca
    24421 caaacttatg tgtatggaca aaaataatt
//
LOCUS       DAOOWJ010000031        24058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2115_length_24058_cov_8.310545, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000031 DAOOWJ010000000
VERSION     DAOOWJ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 24058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24058
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2115_length_24058_cov_8.310545"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..2872)
                     /locus_tag="PLW22_04695"
     CDS             complement(83..2872)
                     /locus_tag="PLW22_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOU74037.1"
                     /translation="MKKILSITLKVLAALVTLFIAAAIVLPIIFKPQLMQVAKKEANS
                     MLNAKVDFTDFQVSLIKGFPNLYVGMKGLTVVGIDSFANDTLVAFKEFGVKVNLWSVI
                     DMTDIEVKSILLDEARVNAHVLPDGRVNWDIMKPSETPETEMEDTAAATTVSTKVSLK
                     RFEIRNANIAYIDDSSNMKATIQNFNLTLSGNMGMSQTELKLKTSIESLNFWMDGIRY
                     LKNSAVGFDAIIAANLDSSYYTFKENAFSINQLKLLFDGSVRMPGNDIDINLNFKTPS
                     TDFKALLSMVPAVYMTDFEGLQASGKFGIEGYVRGTYNDSQMPSAFVAINVDNGMFKY
                     PDLPKSVTDVNISTKIWFDGVNMDNTSVNVDRFSLNLGGNPFSMRMRVATPISDMQVD
                     GNITGQIDFATLSDVIPMDSTYMEGLLESNLDFGGKLSFIEKEQYERFKADGLLKLSR
                     FKFKSPDIPAEVKLNEVTLNFTPQYVNLANFDMSIASSDIRMNGRLENFIPYVFKDET
                     LSGNLNVNSNLLDVNELLTGESAPEEEVTDTTALSVIELPHNINFSTRVDMKKIVYDK
                     LNIENLAGNVALSGGVADLKNLSMNMLQGDVRMSGTYDPRDVKAPVVNFDFDMRNIDI
                     PTAFKSFTSLQKIAPTVKDMTGNVSTQFKFTSMLDTTMMPVLNSINAYGKLQSKSIGV
                     SNSKAFAKLADFLKKEEFRNPSLKDVNVSFSIKNGRIYIEPFDTKIAEAKMNFGGDMG
                     IDQSLNFRAKVSVPSSYLGGVANLANDLLGKYGAKLPQTIDVNLKILGTSSKPEVQLD
                     SGGGSAESSSSVKETAKEAVKEKVDEAKVEARQKAKAEADKLIADAEREAEQIRAEAA
                     KRAEQIRAEAEAQAKKTEAEGQKKGPIAERAAKEAAKKIRQNGEAAAQKVIAEADEKA
                     NAVVTKAKAEAAKLE"
     gene            3065..3280
                     /locus_tag="PLW22_04700"
     CDS             3065..3280
                     /locus_tag="PLW22_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOU74038.1"
                     /translation="MNLIKRIFRFYYEGFKSMTIGKTLWLIILIKLFVMFAVLKVFFF
                     PNILKRNFKTDEERSKYVIEQLTTKPK"
     gene            3289..4851
                     /locus_tag="PLW22_04705"
     CDS             3289..4851
                     /locus_tag="PLW22_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOU74039.1"
                     /translation="MEHFDLSLVNWSRAQFALTAMYHWVFVPLTLGLAFIMAFMETLY
                     YKTGDEEWKRITKFWMTLFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLAI
                     EGIMAFFMESTFIAVMFFGWNKVSKKFHLLSTWLVALGANLSALWILVANAWMQYPAG
                     MFFNPDTARNEMHNFWEVLLSPVSVNKFLHTISSGYVLAALFVVGISAWYLLKGRNVL
                     LAKRSIIVASVFGLITSVFLALTGDGSAYQVAQKQPMKLAAMEGLYEGKEGAPLIVIG
                     ALTPGKQYNDNKDPYLFRIQIPKLLSFLGYRSGDAFVPGVKDIVEGGYTYTDKDGNQK
                     VALSAEQKIERGRIAIQALADYKNAKELGDTTAMAYHRKLLDANFEYFGYGYLNDPKS
                     IIPNVPLTFYSFHIMVGLGFLFIAFFILILYLVFFKEFKINKWLLRTAILMIPLAYIA
                     SQAGWIVAEVGRQPWVIQDILPTVAAVSRIDASAVQVTFFLFFIIFTTLLIAELRIMF
                     TQIKKGPKDGGK"
     gene            4854..6005
                     /locus_tag="PLW22_04710"
     CDS             4854..6005
                     /locus_tag="PLW22_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOU74040.1"
                     /translation="MVDTTYIFLQHYWWFIISLLGALLVFLLFVQGGQTLIYTLGKTD
                     MERTMIVNALGRKWEFTFTTLVTFGGAFFASFPLFYSTSFGGAYWVWMAILFCFVVQA
                     VSYEYRSKPNNFLGPKTFEVFLFINGLFGTVLLGTAVGTFFTGSQFSVAKINLTNISN
                     PVISSWETPWHGLEAALNLHNVALGLAVFFLARVLAILYFMNHINHPEIFARSRKHLL
                     YNAIPFLVFFLTFVIWLFIREGFAVNPTTGDVFMQKHKYLLNLIQMPVVLVLFLGGVV
                     LVLWGIAKSLAKPYTKGIWFTGIGTVLTVLSLFLIAGYNNTAYYPSTFNLQHSLTIFN
                     SSSSKFTLTVMSFVSLLIPFVAAYIWYAWKSINVKKIDEEEMKNETHVY"
     gene            6018..6140
                     /locus_tag="PLW22_04715"
     CDS             6018..6140
                     /locus_tag="PLW22_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74041.1"
                     /translation="MRFLNEILWFASWPVLIYLSYLLSVWAVKLWEKKNPDQVK"
     gene            6171..7172
                     /locus_tag="PLW22_04720"
     CDS             6171..7172
                     /locus_tag="PLW22_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU74042.1"
                     /translation="MNIYKLLKLNRAIRSNRLKLLLIFMVQLLNRRYYSVRIDPIFAC
                     NLRCLMCYFSKSRKAQPKTFSKVELELLAKNLFGNALQVMVGCGAEPTIHPDFIYLVE
                     LAKRHNVPHVGIVTNGQLLSKNDLLTLSSAGLNELTLSVHATQKETYENFMVGAKWEK
                     LIGLLENIKILRDDGKIAFSIRMNFTANPDNIQELEQFFDVFGDYPINTLQVRPIMEI
                     GGAYFKPFSCESISLYNSVIESLFTTCKKHGITLLANTQNPSSQQKSNPVIAQTYKYV
                     SPQVVVDNNFRWQTETYRQFLKRTGWHKAMLKSIFSIRLSNSSTDLAERYGGQYDVL"
     gene            7224..8333
                     /locus_tag="PLW22_04725"
     CDS             7224..8333
                     /locus_tag="PLW22_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOU74043.1"
                     /translation="MEPRIEISPLTEWGLNLNYPLIIAGPCSAESEEQVLTAAHALAR
                     NGNVRVFRAGIWKPRTRPGGFEGAGEKGLQWLKRVKDETGLAVATEVAMPQHLELALK
                     YGIDMVWVGARTTTNPFLVEQLASVLAGTSIPVLVKNPITPDLELWIGTLERLWRSGI
                     TRLAAIHRGFHPYERSRLRNIPKWELAIDLKSRIPSLPIICDPSHIAGAANLVPEIAQ
                     HALDLSMDGLMVEVHPNPAIALSDARQQLTPDAFTNMLSELTFHQNTSVNNEFANFLE
                     QVRNKIDSIDQQIIELLANRMRLVEQIGEYKRTNNVTIFQLRRWEKILESRIECGKRL
                     GLDEEYIKSLLQLVHKESIKKQAEILRGNRLTNGE"
     gene            complement(8337..8594)
                     /gene="purS"
                     /locus_tag="PLW22_04730"
     CDS             complement(8337..8594)
                     /gene="purS"
                     /locus_tag="PLW22_04730"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOU74044.1"
                     /translation="MKFLAEINVMPLKALLDPQGKAVNATLHGIGFKEVSNVRIGKHI
                     NLEIEAANREVAMEKVTEICSKVLSNPVMEGYDFKLTEITE"
     gene            complement(8642..9400)
                     /gene="pssA"
                     /locus_tag="PLW22_04735"
     CDS             complement(8642..9400)
                     /gene="pssA"
                     /locus_tag="PLW22_04735"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOU74045.1"
                     /translation="MNRITRVIRHIPNGITLLNLFSGSLSIVAAFEGRLELAGLLIII
                     SGVLDFLDGFTARLIGAYTQLGKELDSLSDVVSFGVAPAVILYHMLRQSIGISDCHGI
                     FEGHFVLVIPFLVAVFSSLRLGLFNIDERQTSSFIGLPTPANAFLIIGLVFGANSSWS
                     GLFNPIVGSQVVLIVLAIISSFLLVCELPMFSLKIKSLKPSVAYKQLTFLAGAVVFIA
                     IFQKAALSIIIVWYIVLSTIYYLWDKLINKDFQS"
     gene            complement(9425..9988)
                     /locus_tag="PLW22_04740"
     CDS             complement(9425..9988)
                     /locus_tag="PLW22_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU74046.1"
                     /translation="MIDYLVKLDTDLFLFLNGLHSPFWDSVMLFASGKLTWLPFYLLL
                     IYFIARKHKWKTLWWLLAIAVVVLVADQLSVHLFKNVFQRLRPCHNPDLSGVIHLVGR
                     CGGKFGFVSSHAANTFGVAVFLSMLYRNRWAGVGLLIWAAFVSYSRIYLGVHYPADVI
                     VGALLGSVVGFALWKPATWLMERYTKS"
     gene            complement(9991..10983)
                     /gene="obgE"
                     /locus_tag="PLW22_04745"
     CDS             complement(9991..10983)
                     /gene="obgE"
                     /locus_tag="PLW22_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU74047.1"
                     /translation="MSNSNFVDYVKIFCRSGKGGAGSVHFRREKFVPKGGPDGGDGGR
                     GGHIYLKGNSQLWTLLHLKYQRHIFAGDGESGSGARCTGKDGEDVYIEVPIGTIARDA
                     ETNEVIAEITEHGQVEMILKGGRGGLGNWNFRTATLQTPRFAQPGEPAQEGYVILELK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPKIADYPFTTLVPNLGIVRYRDDRSFVMADIP
                     GIIEGAHEGKGLGLRFLRHIERNSMLLFMVPADSDDIYGQYQILVNELKMYNPELLDK
                     KRALAITKCDLVDEQAKKKISKHLPDIPAVFISSVTGEGIERLKDVLWDNLNQQ"
     gene            complement(11244..11639)
                     /locus_tag="PLW22_04750"
     CDS             complement(11244..11639)
                     /locus_tag="PLW22_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74048.1"
                     /translation="MKPKLTEILLVFGLVLAGILKGITSSDKLLLVIIFLSILLSGYY
                     LFFYRNNISGDSMLLNIATRWALSLFPIAFVITFFIPNTGRILSLLYFAFCIIITILK
                     SRNKTSDRYEQIRMYLLLTILSCIGLSFP"
     gene            complement(11995..12597)
                     /locus_tag="PLW22_04755"
     CDS             complement(11995..12597)
                     /locus_tag="PLW22_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74049.1"
                     /translation="MNNQINLNSNSNRIPDSHEQNNSIDIDTLLIKLKNEDARNLQKM
                     KGLKRLFFTMIIIYTLFMIVNPDPDLQLHHRISGLFYVLSFTYFALLFRKYHNEFSKI
                     DYSISSLEMFKKAAERHNLTFSRYLLVLPSLIFIDIAITISEYYRWTSVEPLNRILIV
                     QAILLPIFIMSGFIGYLLWRKRQKPLRDRALQIIKELNKN"
     gene            complement(12590..13102)
                     /locus_tag="PLW22_04760"
     CDS             complement(12590..13102)
                     /locus_tag="PLW22_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU74050.1"
                     /translation="MSREERFKRTVDENKEKIMRICRYYAPSAEDQKDMYQEILINIW
                     KSLDSFRGDSKVSTWVYRIAVNTSLSYAGKQYKLMKLNVDIENTNIKNFLSTEFNDNM
                     LKDDQFQRLQTHLNQLSVIDKAIMGLVLDELTSKEIADIIGITEPNVRIKIHRIKENL
                     RNQMKGENYE"
     gene            complement(13352..13927)
                     /locus_tag="PLW22_04765"
     CDS             complement(13352..13927)
                     /locus_tag="PLW22_04765"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOU74051.1"
                     /translation="MLNIVLFGPPGAGKGTQSQKIIEKYKLQHLATGDMLRAAIREQS
                     PIGVEAQKYIDKGQLVPDEMVINLIAGELDNHHDVPGFVFDGFPRTTVQAKKLDEMLD
                     AKKAPITIMLALEVEYDELLARLLNRGKMSDRSDDQDEGIIRGRLDIYNKTTLPVMDY
                     YKAQGKYRGVNGMGTIDEIFERICQVIDSSR"
     gene            complement(14270..14698)
                     /locus_tag="PLW22_04770"
     CDS             complement(14270..14698)
                     /locus_tag="PLW22_04770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU74052.1"
                     /translation="MFTIRKATLADIEPVYQLICDLEGESLNYSYFAENYRRNLEDST
                     VHYWVACHGEKVIGFLSMHVQRILHHVRLTCELQELNVLPEYRSQGVGSMLMEQAEKL
                     AKELGLEEVELTTRNYRVRTQEFYKRLGYEQTHLKFVKKL"
     gene            complement(14704..15450)
                     /gene="mtnP"
                     /locus_tag="PLW22_04775"
     CDS             complement(14704..15450)
                     /gene="mtnP"
                     /locus_tag="PLW22_04775"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOU74053.1"
                     /translation="MPTIGIIGGSGLDNPDIIKDATTLRVDTPYGNPSSLLRVGTIGS
                     NRVIILARHGHSHEYSPTEVNYRANIYALKYQGADCIIATTACGSLRAEIERGDFVLL
                     DQFIDFTRKRINTYHESFASGAVHTPMADPFDSALRAHILDCAGRVGVKCHPRGTVVT
                     IEGPRFSTRAESMMFRSWGADVINMSVATEVALANELAIPYAAIAMSTDYDCWKTDEP
                     PVSWEEIVRIFEQNAQRMIALLRMVVETLK"
     gene            15862..>16415
                     /locus_tag="PLW22_04780"
     CDS             15862..>16415
                     /locus_tag="PLW22_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74054.1"
                     /translation="MRVADQYFRTIWVDEGQLPDVQIIDQRYLPHEFHIETISSYKQM
                     AIAIKEMQLRGAGLIGVAGAFGVYLAYRDAFQGNQPMVFVDGAIAEILNTRPTAVNLK
                     WAIDIVCSRVASGLTLEEKAHIALDAAKQLADHDAESCRLIGLNGLPLIEEISDRKKG
                     GVVNILTHCNAGWLAFTDYGSALSP"
     assembly_gap    16416..16515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16516..17082
                     /locus_tag="PLW22_04785"
     CDS             <16516..17082
                     /locus_tag="PLW22_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOU74055.1"
                     /translation="WTKTDPFESPLIDDLIYWRQTLHQKGFVGIDSKGIGYGNLSCRT
                     SGNEFLITGSGTGGFAEIGKQHFALVTSFNIHRNTIDSIGPVIASSESLTHAVLYLCD
                     PKIGAVIHVHNLNMWRSLLRRVPKTGKQAEYGTPEMAKEVIRLYSQTDLKSKGILVMA
                     GHEEGIVSFGPDIASAAQTLLYWKEQSL"
     gene            complement(17079..17702)
                     /locus_tag="PLW22_04790"
     CDS             complement(17079..17702)
                     /locus_tag="PLW22_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74056.1"
                     /translation="MILFKSLRELLYVFSVTAYLFFAATNVFSQDSTWVWRVEGSSSL
                     KFFGLIAYQEGRGEQGFGIPLGIGFQKHYLNGRWRVNTSIQAASYRTFAITDVPDSYY
                     RVTTLGIYSDLDILRYYSFSVVLGAGAGLSYSRGITLSWWNENFGQTIQDEPFNRWYP
                     SVYLAYGIRIAPRKSKYAIEIFLPPGGHANKNFYLLHIVRVGVDYKF"
     gene            complement(17699..18700)
                     /locus_tag="PLW22_04795"
     CDS             complement(17699..18700)
                     /locus_tag="PLW22_04795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOU74057.1"
                     /translation="MSSSKIDFRTEVTVNNFPFRISYQTPVLFCGSCFAENIGGIMHS
                     YKMPVQVNPFGVVYNPFSVARVLRNILQNKEYTSNDLHSRNGLWFSFDHYTRFSSESK
                     DECLLKINEACSSAHEFIKKANLIGVTFGTARVYRLVSTGKVVANCHKIPAKEFTHTL
                     LSVDEIVSEWTKLIDEVLAVIPNIRFLFTVSPIRHWKDGPIGNQLSKSTLILAVHRLV
                     EIYSDKAFYFPGYEIMMDDLRDYRFYDSDMLHPSQLAIDYIWQKFSDALIEHQAMQVT
                     EKVHRIQQALSHRPVNPKSQEFKRFVDIILNEVSSIQKQYPFLNFNEEVNKLKSYIV"
     gene            complement(18687..19514)
                     /locus_tag="PLW22_04800"
     CDS             complement(18687..19514)
                     /locus_tag="PLW22_04800"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOU74058.1"
                     /translation="MEPEKQFWQSSSGKKLFAWHIKPAATPKAIILIVHGHGEHSLRY
                     VPWAKRFAYHGFIVQSWDHIGHGQSEGRRGHIRYFEQLLFEIDLAITKITHDYPKLPI
                     ILYGHSMGGNIALNYAINRPTKLDLLVVTSPWLKLAVPPSPVMDVLSKFMNLVAPFIQ
                     IKSTVKPDQISQIPEEVEKYATDPLNHGLITPRLYTSITSSTKYIFGNAQKISIPTLL
                     MHGSGDTITSHKATDELSHIIPKATYIEWSGCYHELHHEQERDEVFNSILNWINEQL"
     gene            complement(19526..19987)
                     /locus_tag="PLW22_04805"
     CDS             complement(19526..19987)
                     /locus_tag="PLW22_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74059.1"
                     /translation="MKNRLNYFPIYIGITYTITILSCGRALPTNDDLKSEILDVESQF
                     CDYVMRHGIAEGFAFFADESATILCGRDSLIKWKNAIYHYYSDTQYKGAKVEWKPEFV
                     QVSKCGDIAYTYTTHGRFQKQMPIHWLCKEYFIRFGSAIPMEPGSTFRINL"
     gene            20135..>20916
                     /locus_tag="PLW22_04810"
     CDS             20135..>20916
                     /locus_tag="PLW22_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74060.1"
                     /translation="MKPLNLRRLQSKMLILLVGISAIVYLIVFVFIIKSIQHDNLKDA
                     HSYVNTLVKETARSFEDQLNQELSITRTLAEAFRAFNNTSQKDWIDLQNRMVSDVLKE
                     HHNIKSLWVNWDLSKIDKTGKSKGRLRCTFYWENDVLKYRQDTVTFDNLSSKSLWVTK
                     NIKGNTILEPYYWKYSNSDKVKIPMTSLVSPISYGNQLVGTVGCDITLTEIKDRVDKL
                     KLFENSYAFLLSNGSVYVSHPDTTVLGKTMKEINAEEDSIHH"
     assembly_gap    20917..21016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21017..21517
                     /locus_tag="PLW22_04815"
     CDS             <21017..21517
                     /locus_tag="PLW22_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOU74061.1"
                     /translation="QGSQTSAKALDAMKMVANKIQVVNDIAFQTNILALNAAVEAARA
                     GEHGSGFAVVAGEVRKLAERSKQAAQDIVNLANSSLATVEEVRNVMEKLAHEVNRIAD
                     HAKQLSFEFDDQQQETNRIGDSMQKLSAVSKLTSDIANDLRTYSEHLLKLAANLKDAS
                     SFYKID"
     gene            21528..22415
                     /locus_tag="PLW22_04820"
     CDS             21528..22415
                     /locus_tag="PLW22_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU74062.1"
                     /translation="MKTDRISLSPLVWGHWRLTEWNLSQRELLYLTQQCIDLGITSID
                     SADIYGNYSCEKLFGNALALEPSVRNHIQIITKCGIRLISGKFPEVTVKHYDYSYNHI
                     VSAVETSLKNFRTDHIDLLLLHRPSPLLNPGEVALAFNHLYSTGKVLHFGVSNFLPHQ
                     VEMLQSFTPIHLVANQIEISPLQVEHLQNGNLNYLMQKHIIPMAWSPLGGGRLVSPKN
                     KTDKRVSKVIHKIANETSLLPEQVAISWLTTHPSGIIPVIGSGKFERIKNAYNALNTK
                     LSIQQWFEVYSASTGEDVP"
     assembly_gap    22532..22631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23040..23585
                     /locus_tag="PLW22_04825"
     CDS             23040..23585
                     /locus_tag="PLW22_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74063.1"
                     /translation="MRNFISFIAIVTILTLSINPSNAQGITVKIVTPNNKTDFCANQT
                     ITLVAQTDMGTDDGINLKWEGDLSIVEKNLGNILILKPNSKGIYTFTVFLKDNEGNEA
                     SSSVTISVNPIFKPILKLKNNKVEIETKGNFSGCSLKYYIGNQMVNEDAFKNAKQPGK
                     YYVLTTSPDGCSASSNIIEIQ"
     gene            23644..>24058
                     /locus_tag="PLW22_04830"
     CDS             23644..>24058
                     /locus_tag="PLW22_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74064.1"
                     /translation="MKAFNTFRKILQSATLLLVVSAFEITPVFGQLTVTIDPSAGQTQ
                     VCQGTAFTLTVNANGVGDPPNFTYTWSGDIHLITIVPPYTWVAYLNTNSPAGIYNFTC
                     EVEDEASNKASASISIEILQSPTASISTTDPTTFCQ"
BASE COUNT         6844 a         5222 c         4779 g         6913 t
ORIGIN      
        1 ataaactaaa tcatctgcgt catttgggct ccatttcaaa cgctaaacac caaataccaa
       61 aagtaatcaa ccaaaattca agctactcca gcttagctgc ttcggcctta gctttggtta
      121 caacagcatt ggctttttca tcggcttcag caataacctt ttgggcagca gcctcgccgt
      181 tctgccgtat cttttttgca gcctcctttg ctgcccgctc ggcaattggg cccttctttt
      241 gaccctcagc ctcagttttt ttggcttgag cctcagcttc agcccttatc tgctctgcac
      301 gctttgctgc ttcggccctt atttgttctg cttccctctc ggcatcggca attaacttat
      361 cggcttcagc cttggctttc tgacgtgcct caaccttggc ttcatcaacc ttttccttaa
      421 cagcctcctt ggctgtttcc tttaccgaag atgagctttc tgcagaacca ccaccggaat
      481 cgagctgtac ctcgggttta gatgaagttc ctaaaatctt aagatttaca tcgatagttt
      541 ggggcagctt tgcaccatac tttccgagca aatcgttggc aagattagcc accccgccta
      601 agtacgacga gggaaccgaa acttttgccc tgaagttaag ggattggtca atacccatat
      661 caccaccaaa gttcatcttg gcctcggcaa ttttggtgtc gaatggttca atgtaaatcc
      721 ttccgttctt tatactgaag ctaacattca catccttaag gcttggattg cggaactcct
      781 cctttttcag gaaatcggct agcttggcaa atgccttaga gttactaacc ccaatgcttt
      841 ttgattgcaa cttaccgtaa gcatttatac tattcaaaac cggcatcata gtggtatcga
      901 gcatggaagt aaacttgaat tgggtcgata catttcctgt catatcctta accgtaggtg
      961 ctattttctg gagcgatgta aacgatttga aagccgtggg tatatcaatg ttgcgcatgt
     1021 caaaatcaaa attaaccaca ggggccttta catcccttgg gtcataggtt cctgacatac
     1081 gaacatcacc ctgtagcata ttcatcgata gattttttag gtcggccacg ccaccagaga
     1141 gcgctacatt acctgcaaga ttttcgatat ttagcttatc gtaaactatc tttttcatat
     1201 caacccgggt agaaaagttg atgttatggg gcaattcaat aacgctaagg gctgtggtat
     1261 cggtaacttc ctcctcggga gcactttcac cagtaagcag ctcattaaca tcgagcaagt
     1321 tcgagttaac gttaagattt ccgctaaggg tttcatcctt aaaaacgtaa ggaataaaat
     1381 tttccagtcg accgttcatg cgtatatcac ttgaagcaat ggacatatcg aagtttgcaa
     1441 ggttaacgta ctggggtgta aagttgagcg taacctcatt taattttacc tctgcaggta
     1501 tatcgggcga cttgaatttg aaacgcgaca atttaagcag gccatcggcc ttaaagcgct
     1561 catactgctc cttctcgata aacgagagtt ttccaccaaa atcgaggttc gactccagca
     1621 atccttccat atatgtgcta tccatcggaa ttacgtccga aagggttgca aaatcaattt
     1681 gaccggtgat gttaccatca acctgcatat cgctgattgg ggttgccaca cgcatgcgca
     1741 tgctgaatgg gtttccgcct aggttcaagg aaaacctatc aacattcacc gatgtattat
     1801 ccatgttcac gccatcgaac caaatcttag tgctaatatt tacatcggtt accgatttgg
     1861 gcaagtcggg atatttgaac attccgttat caacgtttat ggcaacaaat gcgcttggca
     1921 tttggctatc gttataggtt ccgcgaacat acccctcaat gccaaactta ccagaggcct
     1981 gaagtccttc aaaatcggtc atgtacactg ctggcaccat tgataaaagc gccttgaaat
     2041 cggttgaagg agttttaaag tttaggttga tatcgatatc gttaccgggc attcgaactg
     2101 aaccgtcgaa tagcagtttt agctgattga tgctaaacgc attctcctta aacgtgtagt
     2161 agctgctatc gagatttgct gcaataatgg catcgaagcc aactgccgaa tttttcagat
     2221 atctgattcc atccatccag aagttgagcg attcaatact cgtcttcagc ttgagctcgg
     2281 tttggctcat gcccatgtta cctgaaaggg ttaggttaaa gttttgaata gttgccttca
     2341 tgttcgacga atcgtcgatg tatgcaatgt tggcattgcg aatctcaaac cttttaagtg
     2401 atacctttgt gctaacggtt gttgctgcag cagtgtcttc catctcggtt tcgggcgttt
     2461 ccgatggctt catgatatcc cagttaacac ggccatcggg caacacgtgc gcatttaccc
     2521 ttgcctcatc gagcaaaatt gattttacct caatatcggt catatcaatt acactccaga
     2581 ggttaacctt aacgccaaac tccttgaatg ctacgagagt gtcgttagca aagctatcga
     2641 ttcccactac ggttaagcct ttcatgccca cgtaaaggtt agggaaaccc ttaattagcg
     2701 atacctgaaa atcggtaaag tcaacctttg cattcagcat gctgttagcc tctttcttgg
     2761 caacttgcat tagctgtggc ttaaatatta ttggcaatac aattgctgca gctataaata
     2821 gcgtaaccaa cgcagccaaa acctttagag tgatactgag aatctttttc atggctgtat
     2881 aattatttta gcagtataat caatgtttga actatttata caaatttagt gaatcaaact
     2941 gaaaggtggc acttttttta tcattcttaa cagacacatg gcttgaacaa acaccaaata
     3001 caaatgtttc tgacagtgtt gtgccatact aaaaaaagtt taaatttgct taatgcagaa
     3061 tgccatgaac ctcatcaaaa gaatttttcg attctactac gaaggtttta aaagcatgac
     3121 cattgggaaa acgctttggt taataatcct tataaagctc tttgtgatgt ttgccgtact
     3181 aaaggttttc tttttcccaa atattcttaa acgcaacttt aaaaccgatg aggagcggag
     3241 caagtatgta attgaacagc taacaactaa acctaaatag caatacgcat ggaacatttc
     3301 gacctatcgt tagtcaactg gtcgagggca cagtttgccc ttacggccat gtaccattgg
     3361 gtatttgtcc ctctcacgct ggggttggct ttcataatgg cgttcatgga aacgctgtac
     3421 tacaaaaccg gcgacgagga gtggaaacgg ataaccaagt tctggatgac actctttggc
     3481 atcaactttg ccattggcgt ggcaaccggc attattctgg aatttgaatt tggaaccaac
     3541 tggagcaact actcctggtt tgtaggcgac atttttggtg caccccttgc cattgagggt
     3601 atcatggcgt tcttcatgga gtcgaccttt attgccgtaa tgttctttgg gtggaacaag
     3661 gttagtaaaa agtttcactt actctcaaca tggcttgtgg ctctaggagc caacctatcg
     3721 gcactatgga tacttgtggc taatgcctgg atgcaatacc ctgccgggat gttctttaac
     3781 cccgatacgg ctcgtaatga gatgcataat ttctgggagg tattgctttc gcctgtgtct
     3841 gtaaacaaat tcctccatac catatcgtcg gggtatgtgc tggctgccct ttttgttgtt
     3901 ggaattagtg catggtacct tctgaaaggt cgaaatgtat tgctggccaa acgaagtatt
     3961 attgttgcct cagtgtttgg attgataaca tctgttttcc ttgcccttac tggcgatggc
     4021 tcagcgtatc aggtagcaca aaagcagccc atgaaattgg cagccatgga aggtctttac
     4081 gagggcaaag agggtgcccc actaattgta attggcgcac ttacccccgg taagcagtat
     4141 aacgacaata aagacccata cctattccgg attcaaattc caaaactcct atcgttcttg
     4201 ggttaccgtt caggcgatgc ctttgttccg ggtgtgaaag atattgtgga aggtggatat
     4261 acttacaccg ataaggacgg caaccagaag gttgcccttt cggctgaaca gaaaattgaa
     4321 cgtggacgaa ttgctataca ggcgcttgcc gactataaaa atgcaaagga acttggcgat
     4381 accacagcca tggcttacca ccgtaagtta ctggatgcga actttgagta ctttggctat
     4441 ggttacctga acgaccccaa gagcattata ccaaatgttc cgcttacctt ttatagcttc
     4501 cacatcatgg ttgggttggg ttttctgttt atcgccttct ttattctaat actttaccta
     4561 gtctttttca aggaatttaa aattaacaag tggttgcttc gcacggcaat tctcatgatt
     4621 ccccttgcct acattgccag ccaggctggc tggattgtgg ctgaggttgg ccgccaaccc
     4681 tgggtaattc aggatattct gccaacggtt gcagcagtat cacgtatcga tgcgagtgcc
     4741 gttcaggtta cattcttcct gttcttcata atcttcacta cactccttat agcggaacta
     4801 cgaattatgt tcacccaaat taaaaaagga cctaaagatg gagggaaata gccatggttg
     4861 acacaacata cattttctta cagcactact ggtggtttat catatcgcta ctgggtgcgc
     4921 tgctggtttt cctacttttt gttcaaggcg gacaaaccct gatttatacc cttgggaaaa
     4981 cagacatgga gcgaacaatg atagttaacg ctcttggccg gaagtgggag ttcacgttca
     5041 ctacactggt tacatttggt ggtgcttttt ttgcttcgtt tcccctattc tactctacca
     5101 gttttggtgg agcatactgg gtttggatgg ccatactgtt ctgttttgtt gttcaggcag
     5161 tttcctacga ataccgcagt aagcccaaca acttcttggg ccctaaaacc tttgaggtat
     5221 ttcttttcat aaacggactt tttggaaccg tgctacttgg aactgccgtg ggaacattct
     5281 tcacgggttc gcaattctct gttgctaaaa tcaacctcac aaacatcagc aatccagtta
     5341 tttcatcgtg ggaaacgcct tggcatggac ttgaggcagc cctaaacttg cataatgttg
     5401 cccttggtct tgcagtattc ttcctggcaa gggtgttggc aattctttac ttcatgaacc
     5461 atatcaatca ccctgaaatt tttgcccgct cccgcaaaca cttgctgtat aatgccattc
     5521 cgttccttgt tttcttccta acctttgtaa tctggttgtt tatacgcgaa ggttttgcag
     5581 taaaccccac cacaggcgat gtttttatgc aaaagcataa gtacttgctc aacctaattc
     5641 aaatgcctgt tgttctggtg cttttccttg gcggagtggt tcttgtgctt tggggtatag
     5701 caaaatcgct ggctaagcca tacaccaagg ggatttggtt taccggtatt ggtactgtgc
     5761 taactgtact ctcgctcttc ctgatagctg gttataacaa cacagcctac tacccatcta
     5821 cattcaacct gcagcattca cttactattt tcaatagttc atccagcaag ttcactttaa
     5881 ccgtgatgag ctttgtttcg ttgcttattc catttgttgc agcttatatc tggtacgcat
     5941 ggaagagcat caatgttaaa aagatagatg aggaagagat gaaaaacgaa acccatgtat
     6001 actaaaacct actgactatg agatttttaa acgaaatact ttggtttgcc agctggccgg
     6061 tactcattta cttgagctac ttgttatcgg tttgggctgt aaagttatgg gaaaagaaga
     6121 accccgatca ggtaaaatga tgtagatttg cctgcatcaa caatggttta atgaacattt
     6181 ataagctact taaacttaac cgcgctatta ggagtaaccg tttaaagtta ctcctaatct
     6241 ttatggttca gctgctgaac aggcgatact actcggttag gattgacccc atatttgcct
     6301 gcaacctacg ctgcctgatg tgctacttca gtaaaagtag gaaagcgcaa cccaaaacat
     6361 tttcaaaagt agagcttgag ctactggcaa aaaatctgtt tggtaatgcc ctacaggtaa
     6421 tggttggatg cggtgccgag cctaccattc atcctgattt tatctattta gttgagttgg
     6481 ccaaaaggca taatgttccc cacgttggaa ttgttaccaa cgggcaactg ctttcaaaaa
     6541 acgacctgct aacccttagc agtgctgggc taaatgagct tactctctcg gttcatgcta
     6601 cgcaaaagga aacctatgaa aactttatgg tgggcgccaa atgggaaaaa cttataggcc
     6661 ttcttgaaaa tattaaaatc cttagggatg atggcaaaat tgctttttca atacgtatga
     6721 actttacggc taatcctgat aacattcaag agctggagca attttttgat gtgtttggtg
     6781 actacccaat taataccctt caggttagac caataatgga aattggaggt gcctatttta
     6841 agccgtttag ctgcgagagc atttcattgt ataacagtgt aattgaatcg ctgttcacta
     6901 cgtgcaaaaa acatggcatc acattacttg ccaatacaca aaatcctagt agtcaacaaa
     6961 aaagtaatcc agtaattgca caaacataca agtatgtatc gccacaggtt gtggttgata
     7021 acaatttccg atggcaaacc gaaacctaca ggcaattctt aaaacgaaca ggttggcata
     7081 aagctatgct taaatccata ttctcaatcc ggctgagtaa ttcatctacc gaccttgcag
     7141 aacgttacgg tgggcaatac gatgttttgt aaacaaaaca taaattttgt ggttaacttt
     7201 gctgcttgaa tacaaaaact gaattggaac ccagaattga aatatcgccc ttaaccgagt
     7261 ggggattaaa cctaaactac cccctaatta ttgccgggcc atgcagtgcc gagagcgagg
     7321 agcaggtact tacagcggcc catgcgcttg cccgcaatgg gaacgttagg gttttccgtg
     7381 ctggtatatg gaaaccacga acccgcccgg gaggctttga gggtgccggc gaaaagggtt
     7441 tgcagtggct taaacgtgta aaagatgaaa ctggcttggc cgttgccaca gaggtggcca
     7501 tgcctcaaca cttggaactg gcgcttaagt atggaattga tatggtttgg gtgggagcac
     7561 gcacaaccac aaacccattt ctggttgagc agctggcctc ggtacttgcc ggaaccagca
     7621 tcccggtact ggtaaaaaat ccaattactc ctgaccttga gctatggatt ggaacccttg
     7681 aacgcctttg gcgttcaggc atcacacgcc tagctgccat acacaggggt tttcacccat
     7741 acgaacgttc acgcttacgc aacatcccca agtgggagct tgccatcgac cttaaaagtc
     7801 gtatccctag cctacccatt atttgcgatc ccagccatat tgcaggagcg gctaaccttg
     7861 tacccgaaat tgctcagcat gcactcgacc tgtccatgga tgggctaatg gtcgaggttc
     7921 atcccaaccc agcaattgcc cttagcgatg cccgtcagca gcttaccccc gatgccttta
     7981 caaatatgct ttcggaatta actttccacc agaacacatc ggttaataac gagtttgcta
     8041 acttccttga acaggttcgc aacaagatag actccattga ccagcaaatt attgagttgc
     8101 tagcaaaccg tatgcggttg gttgagcaga ttggtgagta taaacggact aataacgtta
     8161 caatttttca gctacgcagg tgggaaaaaa ttcttgagtc gcgcattgag tgcgggaagc
     8221 gtttggggct cgatgaagag tatataaaaa gcctgcttca gctggttcac aaggaatcca
     8281 taaagaaaca ggccgaaata ttacgtggca accgtttaac caacggcgag taagccctat
     8341 tcggttatct cggttagctt aaaatcgtac ccttccatta ctgggttcga gagcaccttt
     8401 gagcatattt cggttacctt ttccatggcc acctcacggt ttgctgcttc aatttcaagg
     8461 ttgatgtgct ttccaattcg aacattggat acttccttga acccaatccc atgtagggtt
     8521 gcattaactg ccttcccctg ggggtcgagc agagccttaa gtggcatcac attgatttct
     8581 gctaaaaact tcattttata tcgatttttc ttcggacaaa attagccaaa atttgcatta
     8641 atcagctctg aaaatcttta tttatcaact tatcccataa atagtagata gttgatagaa
     8701 cgatatacca aacaatgata attgaaagcg ctgccttttg aaaaatggca ataaatacaa
     8761 cagctccagc tagaaaggta agctgtttgt aggccactga tggttttaac gacttgattt
     8821 taagcgagaa catgggtaat tcacaaacca gtaaaaacga ggatataatt gctagaacaa
     8881 tcaggactac ttgcgaacca acaattgggt tgaacaaacc actccagcta ctgtttgcgc
     8941 cgaaaaccaa tccaattatc agaaaggcat ttgctggtgt tggcaatcca ataaacgaac
     9001 ttgtttgtcg ctcatcaata ttgaataaac caagacgaag cgaagaaaaa acagccacaa
     9061 gaaaaggaat tactaataca aagtgcccct caaaaatgcc atgacaatca ctaattccaa
     9121 tgctctggcg aagcatgtga taaagaatta cggctggcgc aaccccaaag ctaaccacat
     9181 cggaaagcga gtcaagttcc ttgcctagct gggtataagc accaattaac cttgccgtaa
     9241 aaccatccag gaaatcaaga acaccggaaa taatgataag caaaccagca agttccagtc
     9301 gaccttcgaa tgctgccaca attgaaaggc tacccgaaaa taggtttagc agagtaattc
     9361 cattgggaat atgtcttata acgcgggtta ttctgttcat atacaaaatt aattcattca
     9421 aattttagct tttggtatat cgctccatca accaggttgc tggtttccat aatgcaaaac
     9481 ccaccacgct gccaagtaat gcacctacaa ttacatcggc agggtaatgc accccaaggt
     9541 atatacggct atacgaaaca aacgctgccc aaatgagcaa accaactcct gcccaacggt
     9601 tcctgtatag catactcaaa aagactgcaa caccaaatgt attggctgcg tgcgacgaaa
     9661 caaaaccaaa cttaccccca catctaccca caagatggat tacaccactt aaatcggggt
     9721 tgtggcatgg ccttaacctt tgaaaaacat ttttaaagag atgtactgag agctggtcgg
     9781 ctacaagaac aacaactgca attgccagca gccaccaaag ggttttccac ttatgctttc
     9841 gggcaatgaa gtagattagc aacaggtaaa acggtaacca tgtgagcttt cccgatgcaa
     9901 aaagcattac actatcccag aaagggctgt gcaacccgtt aaggaaaaga aataggtcgg
     9961 tgtcgagttt taccaggtaa tcaatcataa ttactgttgg tttaggttat cccaaagcac
    10021 atcctttaac cgctcaatac cctcgccggt taccgacgag ataaacacag caggtatatc
    10081 gggcagatgc tttgatattt tctttttagc ctgttcgtcc accaggtcgc atttagtgat
    10141 ggcaagagcc cttttcttat cgagcagttc cgggttgtac atttttagtt cgttcaccag
    10201 tatttggtac tgcccgtaaa tatcgtcgga atcggctggc accatgaaga gtagcatgga
    10261 gttgcgctca atgtggcgta aaaagcgaag tcctaaccct ttcccctcat gggcaccttc
    10321 aataatacca gggatatcgg ccattacaaa cgacctatcg tcgcggtatc gaacaattcc
    10381 aaggtttggt accaaggtag taaaagggta gtcggcaatt ttgggtttgg ctgcactcac
    10441 caccgacagc aatgtcgatt taccggcatt tgggaagcca accagtccca catcggccag
    10501 tatttttaat tccaggataa cgtagccctc ctgtgcgggt tcgccgggtt gagcaaacct
    10561 gggggtttgc agggttgccg tccgaaagtt ccagttaccc aaacctccgc gtccaccctt
    10621 taaaatcatc tctacctggc cgtgctcggt aatttcagca atcacctcat tggtttcggc
    10681 atcgcgggca atggtgccaa tgggcacctc aatgtaaaca tcctcgccat ccttacccgt
    10741 acaacgggca ccactccccg attcaccatc gcctgcaaaa atatgacgtt gatatttcag
    10801 atgaagtaaa gtccacagct ggctgttccc cttcaggtaa atatggcctc cgcggccacc
    10861 atcgccacca tcggggccgc ctttgggtac aaacttctcc ctgcggaagt gtacgctccc
    10921 cgcaccaccc ttgcccgaac ggcaaaatat cttaacgtaa tctacaaagt tcgagtttga
    10981 catgtcctgc aataaactac ccgcaaagtt actttaaaaa ggtggaatgg gaaagcagaa
    11041 caaaaaaatg atgctggcta gcagtaaaac aacaatcgca tggaattcta ttccgcacga
    11101 acgggtagtc atagcataaa tgtaaccgta ttggaaagag cacacgcatc cccccatgcg
    11161 cacgaacatt agaataccga acacttattt aacaaaaatc gggttgttta attatcccaa
    11221 tttagttgaa attgaccata aggttaaggg aacgataggc ctatacacga taaaatggtt
    11281 aatagcaagt acattctaat ctgctcatat ctgtcggatg ttttgtttcg ggattttaaa
    11341 atggttatta taatgcaaaa ggcaaagtaa agcaaactca aaatcctgcc tgtattaggg
    11401 ataaaaaatg ttattacaaa cgcaatgggg aataatgaaa gcgcccagcg ggttgctatg
    11461 tttaatagca tactatcgcc gcttatgtta ttcctgtaga agaataaata gtaaccagac
    11521 agtagaattg aaagaaagat aattactagt agtaatttat cactgctggt aataccttta
    11581 agaattcctg ctaaaactaa accaaacact agcagaattt cagtaagctt tggcttcata
    11641 tttcgtataa attagattgg ttaaatgcat taacattata atcaatttta caaaattcat
    11701 acccacgttg gtgcgagcct caatatgcgt acgatatata caaaaccaac ccaaaaattt
    11761 cagcaaatct agtaaaaaaa atttctcgct cgtgctttcg agcagttttt catctatacc
    11821 ggccaaggcg caatgttagg ttacgctcaa acttgccttt ggaggggcaa ccaacaaaaa
    11881 tggacggacg actttgaaaa aactcacacg gaatacaata acacaggggg gcacatcata
    11941 aatgatacag cattaaaaag agcgcacgcg tcgggggacg cgtgcgaaca agtatcaatt
    12001 tttatttaac tcttttatga tttgtaacgc tctatcgcgc aatggctttt gtctttttcg
    12061 ccagagtaaa tatccaataa atccagacat tatgaaaatt ggtaaaagaa tggcttgtac
    12121 aattaatatg cgatttaatg gttctaccga tgtccatcga taatattctg aaatcgtaat
    12181 cgcaatatct ataaaaataa gcgaaggtaa aacaagtaag tatctggaaa aagttaaatt
    12241 gtgtcgttct gctgctttct tgaacatttc aagggatgat atggaataat caatcttgct
    12301 gaactcatta tgatactttc tgaataataa cgcaaaatat gtaaatgata gcacatagaa
    12361 caaacctgat atcctatgat gaagttgcaa atcaggatcg ggatttacta tcataaataa
    12421 tgtgtaaata attatcattg tgaaaaataa tctttttaga cccttcattt tttggaggtt
    12481 tcgagcatct tcatttttca atttaatcaa taaagtatcg atgtcaatag aattattttg
    12541 ttcatggcta tcgggaattc tattgctatt gctgtttagg ttgatctgat tattcatagt
    12601 tttctccttt catttggttt cgtaaatttt cttttatcct gtgaattttg atacggacat
    12661 taggctcggt gataccaata atatcggcta tttccttgct tgttaattca tcgagtacaa
    12721 gccccattat cgccttatca attacactga gttggttcag atgggtttgc aatctctgaa
    12781 attgatcatc ttttagcata ttatcattaa attcagtaga taaaaagttt ttaatgtttg
    12841 tgttttcaat atcaacattt agtttcataa gcttatactg tttgcctgca taactcaagg
    12901 aagtgttaac agcaattcta taaacccaag ttgaaacctt tgagtcgcca cggaatgagt
    12961 ccagactctt ccatatattt attaatattt cctggtacat atctttttga tcttcagcag
    13021 acggtgcata atacctgcat attcgcatta ttttttcttt gttttcgtca actgttcttt
    13081 taaatctttc ttctctactc atatagttga attatttttt tactttttca tttagacatt
    13141 catcagagta tttcgttaca aaattttttg taaacattta cattagcttc ctcttgttct
    13201 cgtaaatagc atacacgttt gcccagatgg ctatgccaaa ctcaattcgg ggcattaaaa
    13261 tacgcacaag cgtataatga ttaatgatat tcattgaaat aaaataggcc atcgtggttg
    13321 tcgatggcct tgatgtatta attagggttt gttacctgct actgtcaatc acctggcaaa
    13381 ttcgctcaaa tatttcatcg atagtaccca ttccgttaac gccacggtac ttgccctgcg
    13441 ccttgtagta atccattacc ggcagggttg ttttgttgta gatgtcgagc cggccacgga
    13501 taataccttc gtcctggtca tcgctgcggt cactcatttt tccccggttt agcaggcgag
    13561 ctaagagttc atcgtactca acctccaaag ctaacattat ggtaatggga gcctttttgg
    13621 catcgagcat ttcgtcgagt ttcttggcct gtacggtagt tcggggaaat ccatcgaaaa
    13681 caaagcccgg aacatcgtgg tggttatcga gttccccggc aatcaaattg attaccatct
    13741 catcgggaac cagctgccct ttatcgatat acttttgagc ctctactcca attggcgatt
    13801 gttcacgtat tgcagcccta agcatatcgc cagtagccag atgctgcagc ttgtatttct
    13861 caattatttt ttgggattga gtgcccttgc ctgcgcccgg ggggccaaat aaaacaatgt
    13921 taagcatttt acgatatatt gtgttttggt ttattacttt ttagtttcca acctcttacc
    13981 tgctaaaatg cttgaacaaa tataggtaaa caattgtcac ggccaaaaaa ttattggaca
    14041 atttgcgggt tgacttaaag cattaccaca gaaaaatatt ccacgctttt taaagttcat
    14101 taagttttat caaatttgat ttgcagcata tagattattt taccatgcaa tttttaatat
    14161 atagaatttt agtaacagtt cagagatagt aacaggttaa ttgaatgtaa ctaaaacggc
    14221 ctaacaagat gttgtgcata agcaatgtat ccataactaa tattgctctc taaagctttt
    14281 ttacaaactt gagatgtgtt tgctcgtatc ccaaccgttt gtaaaattct tgtgttcgca
    14341 ccctgtaatt tcgggtggta agttcaacct cttcaagacc gagctctttg gcaagtttct
    14401 ccgcttgctc catcagcatg ctgccaaccc cctgcgagcg atactcgggc aaaacgttta
    14461 gctcctggag ctcacaggtt aacctcacgt gatgcagaat acgctgaaca tgcatgctca
    14521 agaaacctat aaccttttcg ccatggcagg ctacccagta atgcaccgtg ctatcttcaa
    14581 gattccttcg gtaattctca gcaaagtatg agtaattgag gctctcaccc tcaaggtcgc
    14641 aaattagctg gtaaaccggt tctatatcgg caagtgttgc ctttcgaatt gtaaacatgg
    14701 gctttacttt aaggtttcaa ctaccatgcg tagtagcgcg atcatgcgtt gagcattttg
    14761 ttcaaaaatg cgaactatct cctcccagct cacgggtggt tcatcggttt tccagcaatc
    14821 gtaatcggtg ctcatggcaa tggcagcata gggaatggcc agctcgttgg ccagggccac
    14881 ctcggtggca accgacatgt taattacatc ggctccccag ctgcgaaaca tcattgattc
    14941 ggcccgggtg gaaaagcgtg gcccctcaat ggtaaccacg gttccccgtg gatgacactt
    15001 aacccctacc ctgcccgcac aatcaaggat atgggcgcgc aatgccgagt cgaagggatc
    15061 ggccatggga gtatgaaccg caccactggc aaaggattcg tggtaggtat taatacgctt
    15121 tcgggtaaaa tcaatgaact ggtcaagtaa aacaaagtcg ccccgttcaa tctctgctcg
    15181 taaacttccg caagcagtag tggctataat gcaatcggca ccctgatact tgagcgcgta
    15241 aatatttgcc cggtaattta cctcagttgg gctatactca tggctatggc catggcgagc
    15301 aagaataatc accctgttac tgccaatagt cccaaccctc agcagcgaag atggattccc
    15361 gtagggagta tctaccctaa gcgtggtggc atctttaata atatcagggt tatcgagccc
    15421 ggagccgcca ataattccaa ttgtaggcat atacattagt attaaacatc attgccgtgt
    15481 agttagatta tttgtcatgc tgagctcgcc gaagcatctc tttactcttt ttctcttcag
    15541 tcctttgatg agatccctcg actttgctcg ggatgacaaa aacccttact tcccttctgc
    15601 tccattgtca tgctgagtcc cgatttatcg ggatagactt caacgaagca tctccttgca
    15661 cttttttctc ttttagcatt gactgagatc cttcgtttca ctcaggatga caaaaggcat
    15721 gtttattttt attaactgaa cgacttttta atttaacata gtaaatttaa tgcaaatcag
    15781 tcaaaaaaag catttccgac tacaattaca ttgatttttt caaacagctt tttacctttg
    15841 acacaacgtt gattaaaaac aatgcgtgta gcagaccaat acttcagaac catttgggtt
    15901 gatgagggcc aactgcccga tgttcaaata attgaccaac gctatctacc acatgagttc
    15961 catattgaaa ccatatcgtc ctataagcag atggccatag ccataaagga gatgcaactg
    16021 cgtggagccg gcctaattgg tgtggctgga gcatttggtg tgtacttggc ttaccgcgat
    16081 gcatttcagg gcaaccaacc gatggttttt gttgatggag ccattgccga aattctgaac
    16141 acccgaccca ccgcggttaa cctgaaatgg gctatcgata tagtttgttc cagggttgcg
    16201 agcggtttaa ctttagaaga aaaggcacac atagcccttg atgcggcaaa gcagcttgcc
    16261 gaccatgatg ctgagagctg ccggttgatt ggcctaaatg ggttgccgtt aattgaagag
    16321 attagtgata gaaaaaaggg cggtgttgtc aatatcctca cccattgcaa tgccggttgg
    16381 cttgccttta ccgattacgg ttcagcccta tcgccnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnntggac caaaaccgat ccctttgaat cgcccttaat tgatgacctc
    16561 atatactggc gtcaaacttt acaccaaaag ggatttgtgg ggattgatag caagggaatt
    16621 ggttacggga acctaagctg ccgaaccagt ggtaacgagt ttctgattac aggaagcgga
    16681 acgggtgggt ttgccgagat tggcaagcag cactttgccc tggttacctc gttcaatatc
    16741 catagaaaca ctatcgattc cattgggccc gtgatcgctt cctcggaatc gttgacacat
    16801 gcggtactgt acctctgcga tcccaaaatt ggagcagtta tacatgttca taacctaaat
    16861 atgtggcgct cactgctgcg ccgtgttcct aagacaggga aacaagcaga gtatggaacc
    16921 cctgaaatgg ctaaggaggt aattaggctc tacagccaaa ccgacctcaa aagcaagggc
    16981 atacttgtta tggctgggca cgaagaaggt atagtatcct ttgggcccga cattgccagt
    17041 gctgctcaaa ccctactgta ctggaaagaa caatcgctct agaatttata gtcaacccct
    17101 accctgacga tatgtaacag gtagaaattt ttgttggcgt gccctccggg tggcaggaat
    17161 atctcaatgg catacttgct tttacgagga gctatgcgaa taccgtatgc taaataaact
    17221 gaagggtacc acctattgaa cggttcatct tggatggttt gaccaaaatt ctcattccac
    17281 cagcttaaag taatacctcg ggaatacgat agtccggccc cggcgccaag gacaaccgaa
    17341 aaggaataat acctgagtat gtctaaatct gaatatatgc ctaaagtagt taccctgtag
    17401 tagctatcgg ggacatcggt aatagcaaag gtcctgtatg atgctgcctg tatggaggta
    17461 tttaccctcc agcgtccgtt taggtaatgc ttttggaatc ctatgcctaa agggatgcca
    17521 aacccctgct caccccgccc ttcctgatac gctattaaac caaaaaactt aaggctactg
    17581 ctaccctcca cgcgccaaac ccatgtggaa tcctgcgaaa agacattagt agcagcaaaa
    17641 aacaaatagg cagttacaga aaaaacatat agcaactctc ttaaactctt gaataaaatc
    17701 atactatata actttttaat ttgtttacct cttcgttaaa gttaagaaag gggtattgct
    17761 tttgaatgct acttacctca ttcaaaatta tatctacaaa ccttttaaat tcttgggatt
    17821 tgggattaac cggtctatgg cttaaagcct gttgtatcct atgaactttt tcagttactt
    17881 gcatagcctg gtgctcaata agtgcatcag agaatttttg ccagatgtaa tcgatagcca
    17941 gttgcgatgg gtgaagcata tcggaatcgt aaaaacggta atcccttaaa tcgtccatca
    18001 ttatttcgta tccgggaaaa tagaatgctt tgtccgagta tatttctacc agcctgtgaa
    18061 cagccagaat aagagttgac ttgctgagct ggttgccaat tgggccatcc ttccagtgcc
    18121 tgatggggct aactgtaaaa agaaaccgaa tgtttgggat taccgccaaa acctcgtcta
    18181 tcagtttcgt ccactctgaa acaatttcgt caaccgaaag cagtgtatgg gtaaattcct
    18241 ttgcaggaat tttgtggcag tttgccacaa ccttacccgt agaaacaagt ctgtaaacac
    18301 gtgctgttcc aaatgttact cctatcaagt ttgccttctt aatgaattca tgggcactac
    18361 tgcaagcctc attaatttta agcaagcatt catctttact ctctgacgag aatcgggtgt
    18421 aatgatcaaa gctgaaccat aagccattcc tgctatgtaa atcgttactg gtgtactcct
    18481 tgttttgcag aatgttcctc aacacccttg caaccgaaaa aggattgtaa accacaccga
    18541 acgggttaac ctgcacaggc atcttgtagc tatgcataat tccacctata ttctcggcaa
    18601 agcacgaacc gcagaacagc acaggtgttt ggtagctaat acggaaaggg aaattgttta
    18661 cggtaacctc ggtacggaaa tcaattttag agctgctcat taatccagtt cagtattgaa
    18721 ttaaaaactt catccctttc ctgctcatga tgcaattcat gatagcatcc gctccattca
    18781 atgtaggttg ccttaggaat tatatgcgat agctcatcgg tagctttgtg cgaggtaata
    18841 gtatcgccgc ttccatgcat gagcagggta ggaatactta tcttttgggc attgccaaaa
    18901 atgtacttgg tagaacttgt gattgatgtg taaagcctag gagtaataag cccgtgattg
    18961 agtggatcgg tagcatactt ctcaacctcc tcagggattt gagagatttg atcaggtttt
    19021 acagttgact taatttgaat aaagggtgca accaaattca taaacttgct aagtacatcc
    19081 attaccggtg atggtggaac tgccaatttc aaccagggcg aggttacaac cagaaggtca
    19141 agtttggttg gtctatttat ggcatagttc agggcaatgt ttcctcccat gctatgcccg
    19201 tagagtatta ttggtagctt tgggtaatcg tgagtaattt tggtaatagc caaatcaatt
    19261 tcaaaaagca actgctcaaa gtacctgata tgccctcttc tgccttcgga ttggccatga
    19321 ccaatatgat cccacgactg gactataaag ccatggtaag caaaccgttt tgcccaggga
    19381 acatatcgca atgaatgttc tccatgtcca tgaacaatca ggataattgc tttgggtgtt
    19441 gctgcgggtt ttatatgcca ggcaaatagc tttttcccgc tcgacgattg ccaaaattgc
    19501 ttttcaggtt ccatatatcg tgcttttata agttaattct aaacgtactt ccaggttcca
    19561 tcggaatggc acttccaaac cgtatgaaat attccttgca caaccaatgt atcggcattt
    19621 gtttttggaa ccttccatgt gtagtatagg tatatgctat atcgccacac tttgaaactt
    19681 gaacaaactc gggtttccat tcgactttag ctcccttgta ttgagtatct gaatagtaat
    19741 ggtaaatagc gttcttccat ttaattaagc tatctctacc acaaagaatt gttgcagatt
    19801 catcggcaaa aaatgcaaaa ccttcagcaa ttccatgccg cataacataa tcacaaaact
    19861 gtgattctac atctaatatt tcagacttta aatcatcgtt agtgggcaat gctctaccgc
    19921 acgatagtat cgtaatagta taggttatac caatgtaaat gggaaaataa tttaatctgt
    19981 ttttcattaa atattaaact ttagagtttt cttcacctgt caaaaaatgc attttacaaa
    20041 ttacaaaaaa aatcgaatta tttgcttaag ttatgttaaa aatttatttt ggcaaggata
    20101 tttccctatc tcacagagtg ttaaatcatt gaccatgaag ccattaaatt taaggcgttt
    20161 acagagtaag atgctaatcc tcctagttgg catctcggcc attgtatatc tgattgtttt
    20221 cgtattcatc attaagagca tacagcacga taaccttaag gatgctcata gctatgtaaa
    20281 cacgctggtt aaggaaactg cccgcagttt tgaggatcag ctcaaccagg aattgagtat
    20341 aacaagaaca ctagctgagg cttttcgtgc attcaataat acaagccaaa aggattggat
    20401 tgacctgcaa aacagaatgg tttccgatgt tcttaaagag catcacaaca taaaatcgct
    20461 ctgggtgaac tgggatttat caaagattga taaaacaggc aaaagcaaag gacgattgcg
    20521 gtgtaccttt tactgggaaa atgatgtgct aaaatatcgg caggatactg taacttttga
    20581 taatctctcg agcaaaagcc tttgggttac aaaaaatata aaaggcaaca ccatacttga
    20641 accttactac tggaagtact ccaactccga taaggtaaaa atcccaatga caagcctagt
    20701 aagccctatt tcgtacggta accaattggt aggtactgtt ggctgcgaca tcaccctgac
    20761 agaaatcaaa gatcgagttg ataagctaaa actttttgaa aacagctatg catttctgct
    20821 atcgaacggt agtgtttacg tttcacatcc cgatacaaca gttctgggaa aaacaatgaa
    20881 ggaaataaat gccgaagagg atagtattca ccatatnnnn nnnnnnnnnn nnnnnnnnnn
    20941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnncaag gcagccaaac atcggccaaa gcactcgatg ccatgaaaat
    21061 ggttgctaat aagattcagg ttgttaacga tatagccttt caaaccaata ttcttgccct
    21121 taacgcagcg gttgaggctg ctcgcgcggg tgaacacggc agtggttttg ccgtggttgc
    21181 cggcgaagtg cgtaaacttg ctgaacggtc gaaacaggct gctcaggata tagtaaatct
    21241 ggcaaactca agccttgcca cagtggaaga ggttcgaaac gttatggaaa agctagccca
    21301 tgaggttaat cgaattgccg accatgccaa gcagctctcg tttgaatttg atgaccaaca
    21361 gcaggaaaca aaccggatag gcgactccat gcaaaaactc tcagctgtta gtaagctcac
    21421 cagcgatata gctaacgacc ttagaaccta ctccgagcac ctgctaaaac ttgctgctaa
    21481 cctgaaagat gcttcatcgt tctataaaat tgattaacct cgggtaaatg aaaactgaca
    21541 ggatatcgct ttcgcctttg gtatggggtc actggcgact aaccgaatgg aatttgagcc
    21601 aacgggagct cctttatctc acccaacaat gtatcgactt aggtatcacc tcgatagata
    21661 gtgccgatat ttatggcaac tactcctgcg aaaaactttt cggtaatgcc ctagccctag
    21721 agccttcggt tcgtaatcac atacaaatta tcaccaaatg cggaatccgt ctcatctccg
    21781 ggaaattccc tgaagtaaca gtaaaacatt acgattatag ctataatcat attgtaagcg
    21841 cagtagagac atccctaaaa aatttcagaa ccgatcatat cgatcttttg ctacttcacc
    21901 gtccatcgcc actactaaac ccgggagagg tggcattggc attcaatcat ctttatagta
    21961 ccggcaaggt tttgcatttt ggggtttcaa actttttgcc tcaccaggtt gagatgctgc
    22021 aatcctttac acccattcac ctggtggcaa accaaattga aatatcacca ttacaggttg
    22081 aacacctgca aaatgggaat ctaaactatt taatgcaaaa gcatatcatt cccatggctt
    22141 ggtcaccact aggtggtggc aggttggtat cgcccaaaaa caaaaccgac aagcgggttt
    22201 ccaaagtcat ccataaaatt gcaaatgaaa cctcattgct acctgaacag gttgccatta
    22261 gctggcttac cactcaccca tctggaatta ttccagtaat tggaagcgga aaatttgagc
    22321 gaataaaaaa tgcctataac gctctgaaca cgaaactttc aattcaacaa tggtttgaag
    22381 tgtactcggc atctactgga gaggatgtac cctgattttt cggaaatttt actcattgcc
    22441 ttttagcaat gattttttca gatacaaaaa cctgttttgc attgagcaaa tttcagattt
    22501 cagatttcag aacctgtccc gcattggcgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22621 nnnnnnnnnn nacctgctcc gctatgtgga gttcaaaatg atactaacac ctgcttttct
    22681 tactttaact tccataccat cagctcctac ctttcaaaat tccacagaaa acaacctttt
    22741 acctgttcat tatcggtcaa agcaaaattc tatttttgga tatttatttg tttatcttat
    22801 tgttatgatg tttggcaaat tctttgttca attatttttg agttgatgta ttttgacgaa
    22861 atacttaggt tttaatgatt atttatatat acctgaatat caatgttata actatttttt
    22921 atcattaaaa cactaatttt attgacttta cgtgttgatt taacaaaaaa atactttaca
    22981 ttcgaattaa atttgggtta ggtgtttttt attgtttaca tttataaatc aattctacca
    23041 tgcgtaactt tattagtttc atagctatag taacaatact aactttgagt attaatcctt
    23101 ccaatgctca gggcataact gtaaaaattg ttaccccaaa taataaaacc gatttttgtg
    23161 ccaatcaaac cataaccttg gtggcccaaa ccgatatggg aaccgatgat ggaataaatt
    23221 taaaatggga aggcgattta tcgattgttg agaaaaacct tggcaatatt cttattctta
    23281 agcccaatag caaaggcatt tacactttta cagttttcct aaaggataat gagggtaatg
    23341 aggcttcaag ctcggttaca ataagtgtta atcctatttt taaaccaatt ttaaagctta
    23401 aaaacaacaa ggttgaaatt gaaactaaag ggaacttttc gggttgcagc cttaaatact
    23461 acatcgggaa ccaaatggtt aacgaggatg catttaaaaa tgcaaaacaa cctggtaaat
    23521 actacgtatt aaccacaagt cccgatggat gttccgcctc aagcaacata attgaaattc
    23581 agtaatattt tgacaaatac aagccaataa tatcaaactc gaatgagtga aaaatataag
    23641 cagatgaaag cattcaatac tttccggaag atattacagt cagcgacctt gctacttgta
    23701 gtatcggcat ttgaaattac accagttttt ggacagctca ctgtaaccat agatccctcg
    23761 gctgggcaaa cgcaggtttg tcaaggtact gcatttaccc taactgttaa tgctaatggt
    23821 gttggcgacc cacctaattt cacctacaca tggtccggcg atatacactt aataacaatc
    23881 gtcccaccat atacttgggt agcctatctt aataccaatt ctcctgcagg catctataat
    23941 tttacatgcg aagttgagga tgaagcttca aataaggcat ctgctagcat tagcatcgaa
    24001 atccttcaat cgcccactgc ctcaatttct acaaccgatc ctacaacatt ttgccaag
//
LOCUS       DAOOWJ010000032        23519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2179_length_23519_cov_8.441570, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000032 DAOOWJ010000000
VERSION     DAOOWJ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 23519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23519
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2179_length_23519_cov_8.441570"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..240
                     /locus_tag="PLW22_04835"
     CDS             25..240
                     /locus_tag="PLW22_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOU74065.1"
                     /translation="MNLKTKVNHFYQWFKAKKRITYPVLAVAIGIVAGYLYYYYIGCN
                     SGSCPITSSPWSSMGIGVLMGWLLVSK"
     gene            complement(271..360)
                     /locus_tag="PLW22_04840"
     tRNA            complement(271..360)
                     /locus_tag="PLW22_04840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Ser,seq:tga)
     gene            615..887
                     /locus_tag="PLW22_04845"
     CDS             615..887
                     /locus_tag="PLW22_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU74066.1"
                     /translation="MNKAQLIDAIAAEAKITKADAKKALDAFIKTTGTALKKGDRVAL
                     VGFGSFSVAKRNARTGRNPQTGKPINIKAKKVVKFKAGSELSGKVN"
     gene            949..>1394
                     /locus_tag="PLW22_04850"
     CDS             949..>1394
                     /locus_tag="PLW22_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU74067.1"
                     /translation="MKLNIDPTDKELRKRLIDYLSQFIYDRRYQLFRSIISNRTRYIT
                     VVLEDIYQAQNASATLRTCDCLGVQDIHIIENSNKFSVDSEVDMGASKWLTLHRYNKL
                     ENNTLEAIKTLREKGYRIVATTPHDNDCTISELDLSKGKVALLFGT"
     assembly_gap    1395..1494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1537..2250
                     /locus_tag="PLW22_04855"
     CDS             1537..2250
                     /locus_tag="PLW22_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU74068.1"
                     /translation="MNCILIDDDEMSRRLIEDYASRVDFINLVRSFSNPIEALSFLQN
                     QNDDVHLVFLDIEMPEMSGIDFLETSSAKPQVVIISSKEKYALKAFDYSVTDYLLKPI
                     TFPRFFKAVSRSYERYNQLRSPFDDDREIFIKKNNSLVRVRYGDILWIEAMENYVIIV
                     TSHERFTIHLTMKSIENQLPSVRFKRVHRSFIVNVGKIETIEDNVISIRSNEGRKTIP
                     IGKSYRESLINELNLIANK"
     gene            2257..3447
                     /locus_tag="PLW22_04860"
     CDS             2257..3447
                     /locus_tag="PLW22_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOU74069.1"
                     /translation="MNVLRQLFLEHLAQTSESPLMLEINKADGVFLFGPDGKKYFDLI
                     SGISVSNLGHGNPMVVKAVQSQAADFMHLMVYGEYIQKPQVELTAQLTRLLPENLNSV
                     YLVNSGSEAVEGALKLAKRFTGRSEIVAFKNAYHGSTHGALSVMGCEEQKRNYRPLLP
                     DVRLLNFNSIADLDHITTRTACVIIEPIQGEGGIRLPNVDYLRKLKQRCSETGALLIF
                     DEIQTGMGRTGSMFAFQRFEVIPDILLLAKAFGGGMPLGAFISSKEIMRVLTHNPILG
                     HITTFGGHPVSCAAAIASLNFLMHEKVVEQVEHKGSILAEALAKHPKVREVRRAGLMM
                     AVELGTFDRVQALIKYGLDHGFILDWFLFCNTAVRIAPPLIITEDECRIVANLIVEGL
                     NAIQ"
     gene            3458..4366
                     /locus_tag="PLW22_04865"
     CDS             3458..4366
                     /locus_tag="PLW22_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74070.1"
                     /translation="MRSVVFLGLFFLPLSLFSQPAFDFVKTEDSLKLVFNSISAETSY
                     SRRDSLNTIFLGMLRSTLEVQSSYTYPFDSILIGKLKSPDGRFRIFTWNIPQPGGYQK
                     YFGLLQVKKADGNVALIELKDDRKSITDVKNEKLPAERWMAALYYQLAQFPTSNGNAY
                     VMLGYDFNNLFTSKKIIDVLTFDSQGTPIFGSPVFNVDGKYRLSRIVFEFNARVNMVL
                     RYNDDSKTIVFDHLSPSSPEFAGDFQYYGPDGSFDGFKLEGTEWVYVRDLDLRNPRRS
                     KPKPVNAPVDIKEPGFLYKPDSSPKK"
     gene            4449..6470
                     /locus_tag="PLW22_04870"
     CDS             4449..6470
                     /locus_tag="PLW22_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOU74071.1"
                     /translation="MTLICFATAGFAISSCDSGKKNDDFKWQIDQFADIRIMRYQIPE
                     FEKLTPQQRVLVYYLSEAAKCGRDIIWDQNYKHNLAVRSILEALYKGYPGERNGEQWN
                     QFMVYLKRVWFSNGIHHHYSNDKIMPGFSAGYFTQLVNQTPDSLFPSELGGKVQIIEK
                     YLPIIFDPNVAPKRVNQEAGVDMVAQSACNYYEGVTQAEAEKFYASLANPADSTPISY
                     GLNSKLVKENGKIVEKVWHLGGMYGKAIERIVYWLEKAATVAENDHQRQVIEKLIDYY
                     KTGDLKKFDEYNVLWVNDLQSRVDFVNGFIENYGDPLGYKASWESVVNFRNDEATRRT
                     QTISGNAQWFEDHSPIDDQFKKKEVKGVTAKVITVAQLGGDCYPSTPIGINLPNADWI
                     RKVHGSKSVTMDNITYAYDQAALGNGFLEEFAWSAQEVELNKKYGYLASSLHTDLHEC
                     LGHGSGQLADGVKGDELKNYGSPLEEARADLFALYYIMDPKMVELGLIPNLDVAKVQY
                     SNYIRNGLMTQLTRVELGKNIEQAHMRNRQLISKWCYEQGKADNVIERKERDGKVYFV
                     VNDFEKLRQLFGQLLKEIQRIKSTGDFEAGKALVENYAVKVDFDLHKQVLDRFAKLNI
                     APYGGFINPRIIPVTQGDSVVDALVEYPDSYVEQMLEYSKRYSYLPLYN"
     gene            7155..7685
                     /locus_tag="PLW22_04875"
     CDS             7155..7685
                     /locus_tag="PLW22_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74072.1"
                     /translation="MDTLDKIRRKIAIYIVDYSIEWSDILFNGLECSEPFYIKKFANG
                     EEFINYLKTEKLSKRITHVALIGYTFYDEKNQTLMNGIEILESLKKIKPFVNAIMLAN
                     EDEQEYGGYVKKLGAVAVINKDDSALLRINNHIALLFSKQALAQRKKDFKIALYIWIG
                     TIIIMASYLLIGYYLI"
     gene            7875..9929
                     /gene="htpG"
                     /locus_tag="PLW22_04880"
     CDS             7875..9929
                     /gene="htpG"
                     /locus_tag="PLW22_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOU74073.1"
                     /translation="MQTGKIGVTTENIFPIIKKFLYSDHEIFLRELISNAVDATQKLK
                     TLASVGDFKGELGDLTIRVKVDRKKGTLTISDSGVGMTADEIDRYINQIAFSGAEEFL
                     EKYKAQANGIIGHFGLGFYSSFMVSDKVEIITRSWQDGAKPVRWSCDGTPEYKIEDAD
                     REARGTDIVLHISDDSKEFLEDHRIEELLRKYCRFLPIPIAFGTEKEWKDGKEVDTGK
                     DRIINETNPLWTRKPAELKDEDYQKFYRDLYPAQEEPLFYIHLNVDYPFNLTGILYFP
                     RIKTNFDIQKNKIQLYCNQVYVTDSVEGIVPEFLTLLHGVIDSPDIPLNVSRSYLQSD
                     SNVKKISSHITKKVADRLQEIFKNDREQFEKKWDDLKLFITFGMITDEKFYERAEKFA
                     LLKNVDGKYFTFEEYEKLVKENQTDKNKTIIYLYASDKDDQHSYIEEAKARGYDVLLM
                     DGQLDPHFINTLEAKLKDTRFARVDSDVIDKLIVKEDRKEVSLPADEQADLRLVFEGA
                     LPAQDHYWVSFEELGEGANPVVITQSEFMRRMKDMSAMSPTSSFYGSMPDSRNLVVNV
                     SHPLVKSIIEAKSKQLSERLNEVNEKIKPVKAKVEELEKAQKGKKDEEIPQADKDALA
                     DAEKQLDKLEEERSTVLKDFGKNYKLVKQLIDLALLANNMLKGEDLSRFVKRSIELIK
                     GE"
     gene            10062..11468
                     /locus_tag="PLW22_04885"
     CDS             10062..11468
                     /locus_tag="PLW22_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74074.1"
                     /translation="MERDILTTKRKALAINLDPTIYGTIAEIGGGQEVARAFFQAGGA
                     SGTIAKTISAYDKVFSDYLYSDGDSGRYVCLKRLQQMLDAEYKQLIDVLSSERCPDKR
                     FFVFANTVETLNFRKDNQGHGWLGVRFELTKQGAANEVVVHVRLNENDAILQQYTLGT
                     LGVNLIYACFYYPKSPTLFLQSLMDNLSTDRVEITMVRMGGPDLDYVDNRLLGVQLVK
                     NGMTPAIFFDRYGNVQEPDDMLYKKNALVLRGRFRPITYVGFDILKTSYAHFKKNALF
                     KPDNTLQICEITLNNLLEEGELKERDFLDRVDLLNGMGQNVLISNFREFYRLVDYFGR
                     FKHKNLRIVMGIPTFVKVLDKSYYANLPGGLLEAVGKMFPARTKFYIYPALDRNTGNI
                     RYITDITIPDDVMHIFHYLKQNDLIVSLTGVNEQWLKITSDEVLSLIQSNDKRWESMV
                     PKYVVKTIKQKKLFGYTG"
     gene            11554..14352
                     /locus_tag="PLW22_04890"
     CDS             11554..14352
                     /locus_tag="PLW22_04890"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOU74075.1"
                     /translation="MENILGNIDPKTIDELYRTWKSNPNSVDEGWQKFFLGFDLAISG
                     SYSAQDCIADSEFKVIKLIEAYRMRGHLFTKTNPVRTRRSYSPTLDIENFGLGTKDLD
                     IRFKAGELIGLPNATLSEIIERLNRIYCRSIGVEYMYLREPKLVDWIQSRVEGTLNHT
                     QFSPDQQKHILYHLTAAVGFEQFIHRKFVGQKRFSLEGLEALIPALDTTIEHGAEHGV
                     KEFVIGMAHRGRLNVITNIMRKPYEEIFAEFIGEAYDDESTLGDVKYHLGYSNTIETD
                     KGKLVRLHLVPNPSHLETVGPVAEGIARARIDDAYQGDVNSLIPIIIHGDAAVAAQGV
                     VYETIQMSRLSGYSTGGTIHIVLNNQVGFTTNYIDARSSTYSTDVAKVTLSPVFHVNA
                     DDPEALLHVIRLAVEFRQTFHRDVFIDLLGYRKYGHNEGDEPRFTQPVLYDLIAKHPN
                     VRDIYTSKLVETKVISVEDAATIRKDYDTMLEERFVLAQANPKIRIGRFIPEKSSGYR
                     YSSSNEFEKSPDTGVSKEMLNMVSNALVELPKGIDFFKKLVKIIDERRENYTLQKVDW
                     AMAELLAYGTLIAEGHPVRLSGQDSERGTFSHRHSVYSIQGSEEKYYPLQNIPNASAK
                     FSVYNSLLSEYGVLGFEYGYSVALPNGLTIWEAQFGDFHNVAQVIIDQYISSAEDKWG
                     LQSGLVLLLPHGFEGQGPEHSSARIERFLTLSARNNMQIVNATTPANFFHVLRRQLKR
                     DFRTPLVVFTPKSILRHPKNISTLQELESGSFLEVIDDSNVDEKAVSRVVFCSGKIYY
                     DLLQRKEELDVKDIALVRIEQLYPFPSRQVEKILDRYPNAKKWLWVQEEPQNMGAWNF
                     VKDRITGVPLEVISRAPSGSPAVGLSKIHNLEQKEIITKVFRPCTCELKNKYCGLQCE
                     EGSRRFNRLNQYEYFERK"
     gene            14375..15643
                     /gene="odhB"
                     /locus_tag="PLW22_04895"
     CDS             14375..15643
                     /gene="odhB"
                     /locus_tag="PLW22_04895"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOU74076.1"
                     /translation="MIVDVRIPSPGESISEVELTRWLVSDGDIVEKDQEIAEVESEKA
                     TLPIIAPEAGKIALVVSAGMIKVNEVCCRIDTSFAGKSPKPMETANSASQSKVEVVEK
                     PKVTTEGKTDFPTHEDKITDKVKVTPLARELMHEYNMNIDDVLNGLRRITAGDVQMVK
                     DTLNNFVVTSDKRNEAPTLVRSEERIRMSQLRKKVSERLVTAKNTTAMLTTFNEVDMS
                     RVQEIRARYQTDFQQKFGVKLGLMSFFVRAAVEALRLHPMTNSMIDGEDIVTPSYIDM
                     GIAVQTPKGLMVPVLRNAQTMSFAEIEKEILRLATKAREGRITLDELNGGTFTITNGG
                     IFGSMLSTPILNYPQSAILGMHNIVDRPVAVNGKVEIRPIMYVALSYDHRIIDGKDSA
                     AFLMDIKRMIENPEKLLFGGDDPIMKLIGL"
     gene            complement(15675..17978)
                     /locus_tag="PLW22_04900"
     CDS             complement(15675..17978)
                     /locus_tag="PLW22_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU74077.1"
                     /translation="MKRKLKIRQKILLYILTVTAILYVASVGYILWQSRANIYSDAIE
                     KTQLTAKVSAKEVGAIFEKELSLVRTLSQAFTVYKNMPHEQWKKLFLDMYIPVLRENP
                     EIYSIWDSWEYKSFVPGYDKDYGRFVITTWREGNEIKWLYDERSINGDPPIYGKYKKL
                     GQEAIWEPYVDQVTKGKADIKLMITYYSPIYINNVFSGIVASDVSLESLQEMVSKIKP
                     VKGSFAYLISNEGIIAAHPSKDFINKKIDEAFKIDNEKEKIAERIAKGEEFYYTKTDE
                     TGKQLLICHSPIIAGKTKTPWSLALSVPVDVIMANAKETLYVSLFVSIAGLLVMVLVL
                     LFVSDNLTKPIKRITDILKRMARGEVSDELKVDLNTGDEIEEMALALNTTIDGIGHKT
                     TFANEIGKGNYTTYLELLSDKDVLGKSLIHMRDSLKKAAEDEEKRKVDDQKRSWANEG
                     YAKFGEILRQNNNDLQKLCDSVIAALVKYVGANQGGIFLWNDEDKTNQYFELVSAFAW
                     DRKKYLTKQIEKGEGLVGACAMEKETIFMTDVPDDYVNITSGLGDANPRCVILVPLKH
                     EDEVLGVIEMASFKVLEKHEIEFIEKIAESIASTILSVKINARTKMLLEQSQMQAEEM
                     KAQEEEMRQNMEELQATQEEVERKSAEIEEFIKSIQSSAYVIEYDLNGNILNVNDSFI
                     QFVGVPREQLIGSHHSDNLELSDVQKKEYGTFWDNLKAGISKKVKSTITWKGKTYHFL
                     ETYIPVKDSEGRVYKIMKLAFEDGEFK"
     gene            18108..21020
                     /locus_tag="PLW22_04905"
     CDS             18108..21020
                     /locus_tag="PLW22_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU74078.1"
                     /translation="MKQILKKIQLLLFAVTVFTANALAQQYKYETVPNDPMGVRIYTL
                     DNGLKVLMSVTKDEPRIQTFVAVRVGSKNDPKETTGLAHYFEHMMFKGTPDFGTLNWE
                     KEKEMINTIEQLFEVYRNETDSLKRAAIYHKIDSISFEASKLAIPNEYDKLMSAIGSR
                     GTNAATSNDYTIYIENIPSNQLENWAMIQANRFSHPVLRLFHTELETVYEEKNMSLTN
                     DARKAGEALLRGLFPNHPYGQQTTLGEAEHLKNPSMKNIREFFDKYYVPNNMAVILAG
                     DFNPDEAIKVVDKYFGTLKAKPLPEFSIVPEEPIIQPKEYEVVGLEAENVSIGFRFAG
                     ATSADADMLSLINSILYNTKAGLIDLNINKKQLALRAYASARVMTDYSILQLGGRNKK
                     GQSLEEVKDLLLKQVELLKKGEFPDWMLEAAINNYKLNRMNALESASSRARLMMNAFL
                     NGIEWENVVNSIERLQKISKDDVVKFANDYLGNNYVVVYKRQGKPQDVAKVAKPPITP
                     IHINRDVESDFLKKVKETKVTPVEPVFIDYNKDFTKIKINKNIEVLYKQNTENGTFNL
                     VYYYPFGSLSNPALTHAADYIQLLGTSKLSAEEVSQEFYRLACTFNINVNDEDTYITL
                     SGLSENAAKALNLMESLLNDCQPDESALKAYIANQIKARNDAKRNQSAVFSALVSYAT
                     YGDNNPQKFILSEKELNELKANDLIGIIKSLNSYRHTVLYYGPLASKDLKVMLSKEHR
                     LPKKFIPTPENKRFEPLETTSDRVLFAHYDAKQSYLQTLSRGQKYDDNLYPIITLYNE
                     YFGGGMNAIVFQEMREKRGLAYTARSRYTIPSRPDDYFINMSYIATQNDKVVDAFNAF
                     NELFNAMPENTIGFNLSKEALISDIRNNRIRNMRVLYSYLNAKQMGWNEDIRKKLYSQ
                     IPTYTFDDVKKFQEQFVKGKPKTYIILGKEADMNFQELEKLYGPVTKVTLEQIFGY"
     gene            21073..22767
                     /gene="ggt"
                     /locus_tag="PLW22_04910"
     CDS             21073..22767
                     /gene="ggt"
                     /locus_tag="PLW22_04910"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOU74079.1"
                     /translation="MGKKILSSILLLTGITLTLFSQDRMTGLNFATRSEVIARNGMAC
                     TSQPLATQVAIDILKQGGTAVDAAIAANAMLGLVEPTGSGIGGDLFAIVWDAKTKKLY
                     GLNASGRSPMSLTLEYFKKNGIAHIPALGPLPVSVPGCVDGWFELHKRFGRLPMQKIL
                     SPAISYAREGFPVSELIAYYWQRNAVRLKQYEGFADVFMPNGKAPSKGEIFRNPYLAN
                     TYELIALKGRDEFYKGSIAKAIDEYMKRNGGFLSYEDLATHKSEWVEPVSSSYRGYDV
                     WELPPNGQGIATLQILNILEQFDIKSMGFGSAEYMHLFIEAKKLAFEDRARYYADPDF
                     VKIPYEILISKEYARERAKLIDLNRAARTYDAGMLQTSNTVYLTVADREGNMVSLIQS
                     NYRGMGSGMTPTGLGFVLQDRGELFSLEEGHPNVYAPGKRPFHTIIPGFITKDGKPWV
                     SFGVMGGGMQPQGHAQIIVNLIDFGMNLQEAGDAPRIQHEGSSEPTGERMTDGGMVYL
                     ESGFDYQEIRKLLAKGHRIGFSLDGYGGYQAIMFDEKNGVYIGASESRKDGQAAGY"
     gene            22915..>23519
                     /locus_tag="PLW22_04915"
     CDS             22915..>23519
                     /locus_tag="PLW22_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU74080.1"
                     /translation="MKKLFLITFVLIGFEAFSTNILTQTVRGVVIDKNTKMTLPGANV
                     ILLDSDPLRGVMTDEQGRFKFESVEIGRVSLKVSFLGYNDVVLSNLNLQVGKELVLNI
                     EMEELAVNVEAVVVTAKSDKSTPMNKLATVSARTFTVEETERYAGSRNDVARMAANYA
                     GVMGVDDSRNDIIIRGNSPMGLLWRLEGVDIPNPNHWGATGT"
BASE COUNT         6891 a         4570 c         5138 g         6820 t
ORIGIN      
        1 acataaatat atatatagat gaatatgaat ttaaagacca aagtgaatca cttttaccaa
       61 tggtttaagg caaagaaaag gattacctat ccagttcttg ccgttgcaat tggaatagta
      121 gcgggttacc tgtactatta ctatataggg tgtaacagtg gtagttgccc aataacctca
      181 tcgccgtgga gtagtatggg gataggtgtg cttatggggt ggttgttggt atcaaaataa
      241 aaaacgaagg gtgttaccct tcgttttttt tggcggagaa agagggattc gaacccccgg
      301 tccgcgtaaa cggacaacgg ttttcaagac cgccgcattc gaccactctg ccatttctcc
      361 gctgcaaaaa tagttgcttt ttcctttttg acaaatagct tgttgaaaaa atgttgaaaa
      421 aagtatactt ttacttgcat tgtatcaaaa aaacgctata aattagcatc atataaagag
      481 aatggttttt aaaaatcaac aaaaatggct atcggggcaa aaaaaaattt caaaaaactt
      541 gcatttatta ataaatgtaa taattttgtc tttgatagtc aactaactat tattaaccct
      601 aaattctatt tgttatgaac aaagcacaat taatcgatgc aattgctgct gaagccaaaa
      661 ttaccaaggc tgatgctaag aaagcacttg acgctttcat caagactacc ggtaccgctc
      721 ttaagaaagg tgaccgcgtt gctctagtag gttttggctc tttctcagtt gctaagagaa
      781 atgctagaac tggccgcaac cctcaaaccg gaaagcccat caacattaag gctaaaaagg
      841 ttgttaagtt caaagctggt agcgaactta gcggaaaagt taactaatta tttgcctttt
      901 tttcaaaaag gagagcctgc attggttctc cttttttatt tttgttcaat gaagctgaat
      961 attgacccca ccgataaaga actgcgcaaa aggctgattg attacctgtc gcaatttatt
     1021 tacgatagaa ggtatcagct attcaggagc atcattagta atagaacacg ctacatcacc
     1081 gttgttcttg aagatatcta ccaggctcag aatgcaagcg ctacccttag aacttgcgat
     1141 tgtttaggcg ttcaggacat acacataatt gaaaatagca acaaattctc agttgatagc
     1201 gaggttgata tgggggcttc caaatggctt actctacacc ggtataacaa actggagaat
     1261 aacacccttg aggcgattaa aaccctaaga gaaaagggat atagaattgt tgctactact
     1321 ccgcacgata atgattgtac cattagtgaa ctcgatttat caaagggaaa ggtcgctttg
     1381 ctttttggta ctgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttttg
     1501 taaattcgta aaaaaatcac agcaaaacaa ttggttatga actgtatact tatagatgac
     1561 gatgagatgt cgagaaggtt aattgaggat tatgcttcaa gggttgactt tatcaatctg
     1621 gttcgctctt tctccaatcc tattgaggca cttagtttcc ttcaaaacca aaacgacgat
     1681 gttcatttgg tttttcttga tatcgaaatg cccgagatgt cgggaatcga ttttctcgag
     1741 accagttcag ctaaacccca agttgttatt atatcgtcaa aggaaaagta tgccctaaag
     1801 gcatttgact acagtgtaac cgattacctt ttaaaaccca ttactttccc tcgatttttt
     1861 aaagcagtat cccgatccta tgagcgttac aaccagttac gttcgccatt cgacgatgat
     1921 agggaaattt ttatcaagaa aaataactcg ctggtacggg ttaggtatgg cgacatcctt
     1981 tggattgagg ctatggagaa ctacgtgatt attgtgacca gccatgaacg gtttaccatt
     2041 catttaacca tgaaatctat tgaaaatcag ctacccagcg tgaggtttaa gcgtgttcat
     2101 cgctccttta tagttaatgt tggtaaaatt gaaaccattg aggataacgt aatatcgata
     2161 cggagcaacg aaggtcgtaa aaccattccc attgggaaat cgtatcgcga gtcattgata
     2221 aacgagctaa atctaattgc aaataagtag gacgaaatga atgtgttgag gcagctcttt
     2281 ctggagcatt tagcccaaac atcggaatcg cccctaatgc ttgagataaa taaagctgac
     2341 ggggtttttc tatttgggcc agatggtaaa aagtatttcg atttaatttc cggtatctca
     2401 gtaagtaacc ttggtcatgg aaaccccatg gtggttaaag ctgttcagag tcaagcagcc
     2461 gatttcatgc accttatggt ttacggggag tacatccaaa aacctcaggt tgagctcacc
     2521 gctcagctta ccaggttact gcctgagaat ttaaattcgg tttaccttgt taactctggt
     2581 agcgaggccg ttgagggtgc gcttaagcta gcaaaacgat ttaccggtag gagtgagatt
     2641 gtagcattca aaaatgcata ccatggaagc acacatggcg ctttaagcgt aatgggttgc
     2701 gaggagcaga agcgaaacta ccgcccctta ctccccgatg tacgtttatt aaactttaat
     2761 agtatagccg atttagatca tataacaacc cgaacagcct gcgtgatcat tgagcccata
     2821 cagggcgaag gaggtattcg attacctaac gttgattacc ttcgtaagtt gaaacaacga
     2881 tgcagtgaaa cgggtgcatt acttatattt gatgagatac agactggaat ggggcgtact
     2941 ggaagcatgt ttgcttttca aagatttgag gttatccctg acattctgtt actggcaaaa
     3001 gcctttggtg gtggaatgcc attgggggcc tttatttcat caaaagaaat tatgagggta
     3061 ttaacccaca acccaattct cggacatata actacttttg gggggcatcc ggtttcgtgc
     3121 gctgcagcca ttgcatcgct caactttttg atgcatgaga aagttgttga acaggttgaa
     3181 cataaaggct caatccttgc cgaggctttg gcaaagcatc caaaggttag ggaggtgcgt
     3241 cgagctggac ttatgatggc tgttgagctg ggaacgtttg acagggttca ggcgttgatt
     3301 aagtatggcc tcgaccatgg attcattttg gactggtttt tgttctgtaa tacagcagtt
     3361 cgcattgctc cacctctaat aattacagag gatgaatgcc gaatcgttgc aaatctgata
     3421 gttgagggtt taaatgctat tcaataaagt ttctgttatg cgcagtgtag tttttttggg
     3481 attgtttttt ttgccgcttt cacttttttc tcagccagca ttcgattttg ttaaaactga
     3541 ggattcatta aagttagttt ttaacagtat ttcagcagaa acctcctact ctcgtcgcga
     3601 ttcgttgaat acaatatttt taggtatgct caggagtacc ttggaggttc aaagctcgta
     3661 tacttatcct tttgattcaa tcctaatagg gaaattaaaa tcgcccgatg gcagatttcg
     3721 tatttttacc tggaatatcc cacagccggg tggttaccaa aaatattttg ggttattgca
     3781 ggttaaaaag gccgatggaa atgtggcttt gattgagctt aaggatgatc gaaaaagtat
     3841 taccgatgta aaaaacgaaa agctacctgc tgaacgatgg atggccgcgc tttactatca
     3901 attggctcag ttcccaacgt ctaacggaaa tgcttacgtt atgttgggat acgatttcaa
     3961 taatcttttt acatcaaaaa agattattga tgttctaact ttcgatagcc aaggtactcc
     4021 aatttttggc tctccggttt tcaatgttga tggtaagtat aggctatcgc ggattgtatt
     4081 tgaattcaat gcccgagtaa acatggtgtt acgatacaac gatgatagca aaaccattgt
     4141 tttcgatcat ctttcacctt caagccctga atttgcaggc gactttcagt attacggccc
     4201 cgatggctca ttcgatggtt ttaagcttga gggtactgaa tgggtgtatg taagagattt
     4261 ggatttacga aatccacgtc gatcaaaacc taagccggta aacgctccgg ttgacattaa
     4321 agagccggga ttcctttaca agcctgactc atcacccaaa aaatgattat ggtttacttt
     4381 tttgctaatt atctttagta taatagctaa aaacaatcaa actaaccgtt aaataaatga
     4441 aaaggctaat gactttaatt tgttttgcaa ctgccggatt tgcaatttcc agttgcgact
     4501 caggaaaaaa gaacgatgat ttcaaatggc aaatcgatca gtttgccgat atacgaataa
     4561 tgcgttacca aattcctgag tttgaaaaac tcacacccca acagcgcgtg ttggtttact
     4621 acttaagcga ggctgccaag tgtgggcgcg atattatttg ggatcagaat tataagcaca
     4681 atctagccgt gcgctcaata ctggaggcat tatataaagg gtatcctggc gaacggaatg
     4741 gtgagcagtg gaaccagttt atggtttatt tgaaacgggt ttggttctca aacggaatcc
     4801 atcaccacta ctcgaacgat aaaattatgc ctggttttag cgctgggtac tttacacagc
     4861 ttgtaaatca aacccctgat tcactctttc cttcggagtt gggaggtaag gttcaaatca
     4921 tcgaaaagta tttgccaatc atttttgacc ctaatgttgc tcccaaaagg gttaaccagg
     4981 aagctggtgt tgatatggtg gctcagtctg cctgtaatta ctatgaggga gttacccaag
     5041 ctgaagccga aaagttttat gccagcttgg caaaccctgc cgattcaact ccaatatcct
     5101 atgggttgaa ctcaaaattg gtaaaggaga acggaaagat tgttgaaaag gtttggcatt
     5161 taggcggtat gtatggtaaa gcaattgaac gaattgttta ctggctggag aaagccgcta
     5221 cggttgctga aaacgaccac cagcgccagg tgatagagaa actgatagat tactacaaaa
     5281 ccggcgacct taaaaagttt gatgagtaca atgtactttg ggttaacgat ttacaatcca
     5341 gggttgattt tgtgaatgga tttattgaga actacggcga tcccttaggt tacaaggcaa
     5401 gctgggagtc ggttgtaaat ttcagaaacg atgaggcaac ccgccgcact caaacaatta
     5461 gcggaaacgc tcaatggttt gaggatcact cgccaattga tgatcagttc aagaagaaag
     5521 aggtaaaggg agtaacggct aaggtaatta ccgttgctca gctgggaggc gattgttacc
     5581 cttcaacccc tataggtatt aatttgccca atgccgattg gatccgtaaa gttcatggtt
     5641 caaaatcggt taccatggat aacattacat acgcctacga tcaggcggct ctgggaaatg
     5701 gtttcctgga ggagtttgcc tggtcggcac aggaggttga gctaaacaaa aagtatggat
     5761 acctggcaag cagcctacat accgatttgc atgagtgcct tggccacgga tcaggccagc
     5821 tagccgatgg agttaaaggc gatgagctta aaaattacgg ttcgccgttg gaggaggccc
     5881 gtgccgatct gtttgctctt tactacataa tggaccccaa aatggttgaa cttggcctaa
     5941 ttcccaatct ggatgtggct aaggttcagt attctaatta tatacgaaat ggtcttatga
     6001 ctcagcttac ccgtgttgag ctgggtaaga acatagaaca ggcccatatg cgtaatcgtc
     6061 agttaatttc aaagtggtgc tatgagcagg gaaaagccga taatgtaatt gagcgaaaag
     6121 agcgcgatgg caaggtttac tttgtggtta acgattttga gaagctccgt caactttttg
     6181 ggcagcttct taaagaaatc cagcgaatta aatcaactgg cgattttgag gccggtaaag
     6241 ctttggttga gaactacgcc gttaaggttg atttcgattt acataagcag gttctcgata
     6301 ggtttgcaaa gcttaacatt gcaccctatg gtggttttat taacccccgg attatcccgg
     6361 ttactcaggg cgacagtgtg gtagatgcct tggtggaata ccccgatagc tatgttgagc
     6421 agatgttaga atattccaag agatattcat atctacccct gtataactaa cataacttta
     6481 atttttaaag ccgggtaatc ccggcttttt ttatcaaatt taagtttgga atttccttag
     6541 ataagtcaat agaaacaagt tatttattgt tttttagggg tgtttctatt gactaaattc
     6601 ctctaaaaat gtttctgtat tcacattttt attagcccgg ttgtatttgt taattcatgg
     6661 caatttggtt tagccgttca ctttttacta ttcctctaac ttcctctgac ttcatttaac
     6721 ttcctttaac ttcctttaac ttcctttaac ttcctttaac ttcctctaac ttcctataac
     6781 ttcctcaaac ttcttctaac ttcttctaac ttcttctaac tccataatat tctatctatt
     6841 ccgactcatt ctgactcatt ccgattcatt ccgccttaaa tcttgcgttt cacggttcac
     6901 ggctcacgtt aacggactca aacttcctct aacttcttct aactccataa tattctatct
     6961 attccgactc attccgactc attcctctaa aattagcccg gttgaatttg ttaattcatg
     7021 gcacttcggt ttaaaatttt ataaatttgt attacaatgt agtttagcct ttactcatat
     7081 tgaaatgaat aatactttta ccgcatcgat ttagtatata taaccaaaat aactgtggtc
     7141 aacacatggc aggaatggat acccttgata aaataagaag gaaaatagca atctacattg
     7201 ttgactacag cattgagtgg tccgacatcc tttttaacgg cttggaatgc tctgagcctt
     7261 tttatatcaa aaaatttgca aatggcgagg agtttattaa ctatttaaaa accgagaagc
     7321 ttagcaagcg cataactcat gttgccctta ttggctatac attttacgat gaaaagaatc
     7381 aaaccctgat gaatggtata gagattcttg aatcgctcaa aaagattaaa ccctttgtaa
     7441 atgctattat gcttgctaat gaggacgaac aggagtatgg tggttacgtt aaaaaacttg
     7501 gtgctgtggc tgttatcaat aaggacgata gcgcactact ccgaataaac aaccatatcg
     7561 cattgctatt cagcaaacaa gccctggcac aacgtaagaa agattttaaa atagcgttgt
     7621 atatctggat tggaacaatc attatcatgg cgtcatatct acttattggt tactatttaa
     7681 tctgacaaaa ttgcatacgc ttgtctaatt gacattttct tgtcaactcc attgcgcttt
     7741 gtcattatat tgactatcag gttgatactc aaaaattcat tttgatatct ttatcaacgc
     7801 ccttgttggc attggaattg aaaagttggg taaggtatgt atcgataatg tttaacctaa
     7861 aatattgatg tacaatgcaa acaggaaaaa ttggcgtaac cactgagaac attttcccaa
     7921 taataaaaaa gtttttgtat tctgaccatg agatattttt aagggagtta atttccaacg
     7981 ccgttgatgc cacccaaaag ctaaaaactt tagcatcggt tggcgatttt aagggagagc
     8041 ttggcgacct taccatcagg gtgaaggttg ataggaaaaa gggaacactc acaattagcg
     8101 atagtggtgt gggcatgacc gccgatgaaa ttgaccgtta tatcaatcaa attgcttttt
     8161 caggtgccga ggagtttttg gaaaagtata aagctcaggc taacggtatt attggccatt
     8221 tcgggcttgg attctactca tcgttcatgg ttagcgataa ggtggagatt attacccgtt
     8281 catggcagga tggcgccaaa ccagttcgct ggagttgcga cggtacacct gagtataaaa
     8341 ttgaagacgc tgaccgtgaa gctcgcggaa ccgatattgt tttacatata agcgacgatt
     8401 caaaggaatt ccttgaagat cataggattg aggaattgct tcgcaagtat tgtcggttcc
     8461 tgcccattcc catagccttt ggaaccgaaa aggaatggaa ggatggcaag gaggttgata
     8521 ctgggaagga tagaataatt aacgaaacca atccgctctg gactcgcaaa cccgccgagt
     8581 tgaaggatga ggactaccaa aagttttacc gcgatttata tcccgcccag gaggagcccc
     8641 ttttttacat tcacctgaat gtggattacc ctttcaatct aacaggtatt ctttacttcc
     8701 caagaataaa aactaacttc gacattcaaa agaataaaat tcagctatac tgcaatcagg
     8761 tttacgtaac cgattcggtt gaaggtattg tgcctgaatt tttgacattg cttcatggcg
     8821 ttatcgattc gcccgatatt ccattgaacg tttcccgtag ctacctgcaa agcgattcga
     8881 atgtaaagaa aatatcatcg catattacta aaaaggttgc cgataggctt caggaaatat
     8941 tcaagaatga ccgtgaacag tttgagaaga aatgggacga ccttaagctg ttcatcacat
     9001 ttggcatgat taccgacgaa aagttctatg aacgtgccga gaagtttgct ttactcaaga
     9061 atgttgatgg taaatatttc acctttgagg agtatgaaaa gctggtaaag gaaaaccaaa
     9121 ccgataagaa caaaaccatt atttacctgt atgccagcga caaggacgat cagcacagtt
     9181 acattgagga ggcaaaggct cgtggttacg atgtgctgct gatggacgga caacttgatc
     9241 ctcatttcat aaacactttg gaggccaagc tgaaggatac ccgatttgcc cgtgtcgatt
     9301 ccgatgtgat tgacaagctc attgttaagg aggaccgaaa ggaggttagc ttacccgccg
     9361 acgaacaagc cgatctaagg ttagtttttg agggtgcgct acccgctcag gaccactact
     9421 gggtatcgtt tgaggaactt ggcgaggggg caaatccggt ggttatcacc cagtccgagt
     9481 tcatgcgccg catgaaggat atgtcggcca tgagcccaac ttcaagcttt tatggtagca
     9541 tgcccgatag ccgtaacctt gtggttaacg tgtcgcaccc cttggttaaa tccattattg
     9601 aggctaaaag caagcagctt agcgaaaggc tcaacgaggt taacgaaaaa ataaagcctg
     9661 ttaaggctaa ggttgaggag ctggaaaaag ctcagaaagg taaaaaggac gaggagatac
     9721 cccaagccga taaggatgcc cttgccgatg ccgaaaaaca gctcgataag ctggaggagg
     9781 aacgtagcac agtcctaaag gactttggta aaaattacaa gttggtaaag cagcttatcg
     9841 atctggcatt actggctaac aacatgctta agggtgagga cttaagccgg tttgtgaagc
     9901 ggagcattga actgataaaa ggagaatagt aatcacataa attatttggt tttggctcta
     9961 caattcgtag ggcctttttt ttattgatgt acaaaatgtg ttagccacaa aaagtttgaa
    10021 caaactacct ttttttttgt aattttatac aaaaccatca catggaacgc gatatactta
    10081 ccaccaagcg taaggcttta gccataaacc ttgatccaac aatttatggt acgattgctg
    10141 aaattggcgg agggcaggag gtggcacgtg cattcttcca agcgggtggg gcatcgggaa
    10201 ctattgctaa aaccatctcc gcatacgata aggtgtttag cgattacctg tatagcgatg
    10261 gcgatagcgg acggtacgtg tgcctaaaac gcttacagca aatgcttgat gctgagtaca
    10321 aacagctaat tgatgtgctt agctctgaaa gatgtcccga taaacgtttc tttgtttttg
    10381 caaatacggt ggaaacgctt aacttccgaa aggataacca gggacatggt tggcttggag
    10441 tacgctttga gcttaccaag caaggcgctg ctaacgaggt ggttgttcac gttcgcctga
    10501 atgagaacga tgctattttg cagcagtata ccttaggaac tcttggcgta aaccttattt
    10561 atgcctgttt ttactaccct aaaagcccaa ctctattcct acagtcgctt atggataatc
    10621 tttcaaccga tagggttgag attaccatgg ttcgaatggg tgggcctgat ctggattacg
    10681 tggacaatag gctcttgggg gttcaactgg taaagaatgg tatgacccct gcgatttttt
    10741 tcgatcggta tggcaatgtt caggagcccg acgatatgtt atataagaag aacgcattgg
    10801 tacttagggg acgcttcaga ccaattacct acgtgggctt cgatatatta aagacttctt
    10861 atgctcattt taagaaaaat gcattgttta aacccgataa caccctccag atctgcgaaa
    10921 tcacacttaa taatctgctt gaagagggcg aactcaaaga acgcgatttc ctggatcggg
    10981 ttgatttgct aaatgggatg gggcaaaacg ttttaatatc aaattttagg gagttttata
    11041 gattggttga ttactttgga aggtttaagc ataaaaacct tagaatagtt atgggtatac
    11101 caacttttgt gaaggtgctc gataaatcct actatgcaaa tttacccgga ggcttacttg
    11161 aggctgtagg caaaatgttt cctgcccgta caaagttcta tatttatcct gcacttgacc
    11221 gaaacactgg caatattagg tacatcaccg atattacaat acccgatgat gtgatgcaca
    11281 ttttccatta cctgaagcag aacgatttga ttgttagcct aacaggcgta aatgagcagt
    11341 ggcttaaaat aacctcggat gaggttttaa gcctcattca aagcaacgat aagcgatggg
    11401 aaagcatggt gcccaagtat gtggttaaaa ctataaagca gaaaaagctg tttggttaca
    11461 ccggttgact ttgcacaaat ctttttactc aaaattgagg cttattttga actttactat
    11521 ctgcttagtg ttggttattg aaaatttttg agtatggaaa acattcttgg taatatcgat
    11581 cctaagacta ttgatgaact ttaccggacg tggaagagta atccaaattc agtagatgag
    11641 ggctggcaaa agttcttttt gggtttcgac ctggctattt ctggctctta cagcgcacag
    11701 gattgtattg ctgactcaga attcaaggtg ataaagctaa ttgaagcata ccgtatgcgg
    11761 gggcacctgt tcacaaagac caacccggta cggactcgcc gtagctactc tccaaccctt
    11821 gatatcgaaa actttggttt aggtacaaaa gatttagaca tccgatttaa agctggtgaa
    11881 ctaataggtt tgcctaacgc tactctttct gaaatcattg aaaggctaaa caggatatac
    11941 tgccgtagta ttggtgttga atacatgtac ttgcgggaac caaaactggt tgattggata
    12001 cagagtaggg tagagggaac cctaaaccat acccagttta gccctgacca acagaagcat
    12061 attctttatc acctaaccgc agctgtagga tttgagcagt ttatacatag gaagtttgtt
    12121 ggacaaaagc gattctcgct ggaagggctt gaggcactta tacctgcact tgatactacc
    12181 attgaacatg gcgctgagca tggggtaaag gaatttgtaa ttggtatggc tcacagagga
    12241 cgtttaaacg tgataaccaa cattatgcgt aaaccctatg aggagatttt tgccgagttt
    12301 attggtgagg catacgacga tgaatcaacc cttggcgatg ttaagtacca cttaggctat
    12361 agcaatacca ttgaaaccga taagggtaaa ttggtaaggt tacaccttgt tccaaaccct
    12421 tcgcaccttg aaactgttgg cccggttgcc gaaggtattg ctcgcgcccg aattgacgat
    12481 gcctaccagg gcgatgttaa tagtttgatt ccaataatca ttcatggtga tgcggctgtt
    12541 gctgcccagg gtgtggtata cgaaaccatt cagatgtcgc ggctaagtgg ttactccact
    12601 ggtggaacta tacatattgt gctaaataac caggttggct ttactacaaa ctatattgat
    12661 gcccgtagca gtacctactc aaccgatgtg gcaaaggtaa ccctttcgcc cgttttccac
    12721 gtcaatgccg atgaccctga ggcattattg cacgtaattc gtttagctgt tgagtttcgt
    12781 caaacttttc accgcgatgt ttttattgat ttgctgggct accggaagta tgggcacaat
    12841 gagggcgatg agccccgctt tacccagcct gtactttacg atttaattgc aaagcatcca
    12901 aatgtgcggg atatttatac ttccaagttg gttgaaacta aggtgatatc ggttgaggat
    12961 gccgctacca tcaggaagga ttacgatacc atgctcgagg aacgttttgt gttagctcag
    13021 gcaaatccaa aaatcaggat agggaggttt attcctgaaa aatcaagtgg ttataggtac
    13081 tcatccagta atgaattcga aaaatcgccc gatacaggtg taagcaagga aatgcttaat
    13141 atggtctcca acgctttagt tgagttaccc aaaggaattg atttttttaa aaagttagtt
    13201 aagataattg atgagcgacg ggagaactat accctgcaaa aggttgattg ggcaatggct
    13261 gaacttttag cttacgggac gcttatagcc gaggggcatc cggtgcgctt aagtgggcaa
    13321 gattctgaga ggggaacttt ttcacatcgt cactcagtgt actcaattca gggaagcgag
    13381 gaaaagtatt acccgcttca aaatattcca aatgctagtg caaagttttc agtttacaat
    13441 agtctcctct cggagtatgg ggttttaggt tttgagtatg gctattctgt ggctttacct
    13501 aatgggttaa ccatatggga ggcacagttc ggcgattttc ataatgttgc tcaggttatt
    13561 attgatcagt atattagttc agccgaagat aagtgggggt tgcaatcagg gcttgtgctt
    13621 cttttacccc atggttttga agggcaaggg cctgagcatt ccagcgctcg aatagagcgg
    13681 tttcttacac tttctgcgcg caacaacatg caaattgtaa atgctacaac acctgccaac
    13741 tttttccatg tattacggag gcagctaaag cgcgatttca gaaccccatt ggttgtattc
    13801 acacccaaaa gtatacttcg tcaccctaaa aatatttcaa cccttcagga actcgagagt
    13861 ggcagtttcc tagaagtgat tgacgacagt aatgttgacg aaaaagctgt atcgcgagtt
    13921 gtattctgca gtggaaaaat ttactatgat ctactgcagc gtaaggaaga acttgatgta
    13981 aaagatattg cattggttcg gattgagcag ctatatcctt tcccatctcg tcaagttgaa
    14041 aaaatactcg atagatatcc aaacgcaaag aaatggctct gggttcagga agagccccaa
    14101 aatatggggg catggaactt tgtaaaggat aggataactg gtgtacctct tgaggttatc
    14161 tcaagggcgc caagcggaag tcctgctgtt ggtttaagta agattcataa cctggagcaa
    14221 aaggagataa tcactaaggt atttagaccc tgcacctgtg agctaaagaa caagtattgc
    14281 ggtttgcagt gcgaggaagg gtcgcgtcga ttcaataggc tcaatcagta cgaatatttc
    14341 gaaagaaagt agttaactat aaaatattgc atttatgatc gtagatgtaa gaattccctc
    14401 gcccggcgag tcgatatcgg aggttgaact cacgcgctgg cttgtatccg atggtgatat
    14461 tgttgaaaaa gatcaggaaa tagccgaagt tgaatcggag aaagcaacac taccaatcat
    14521 tgctccagaa gcaggaaaaa tcgccctagt tgtttcagct ggcatgataa aggtcaatga
    14581 ggtttgctgc agaatagata catcttttgc agggaaatct cccaaaccca tggaaactgc
    14641 aaacagtgcg agccaatcaa aagtagaagt agttgaaaag cctaaagtaa caaccgaggg
    14701 aaaaacagat tttcccacac atgaagataa gattactgat aaagtaaagg ttactccgct
    14761 ggcacgagaa ctcatgcatg agtataatat gaatattgat gatgttctta atggcctcag
    14821 gaggataacg gctggtgatg tacaaatggt taaggataca ttaaataact ttgtagttac
    14881 ctctgacaag cgcaatgaag caccaacctt ggtacgttcc gaggagcgca ttagaatgtc
    14941 gcagcttagg aaaaaggtta gcgagcgttt ggtaaccgct aaaaacacca cggcaatgct
    15001 tacaaccttt aacgaggttg atatgagccg tgtgcaggag attagagcaa ggtaccaaac
    15061 cgattttcag caaaagtttg gtgttaagtt agggttgatg tcgttctttg tgcgggctgc
    15121 agttgaagcg ctgaggttac accccatgac caactctatg attgatggcg aagatatagt
    15181 aaccccaagt tacattgaca tggggattgc agttcaaacc cctaaggggc taatggttcc
    15241 tgtgcttcgt aatgcacaaa ccatgagttt tgctgaaatt gaaaaagaaa ttctcaggct
    15301 tgccaccaag gcaagggaag ggcgaattac cctcgatgag cttaatggag gaacatttac
    15361 cattaccaat ggtggtattt ttgggtcaat gctttccacg cccattctta actacccaca
    15421 atcggcaata ctgggcatgc ataacattgt tgataggccg gtagccgtaa acggcaaagt
    15481 ggaaattagg cctattatgt atgttgccct atcgtatgac catagaatta ttgatggtaa
    15541 ggattcagca gcattcctga tggatatcaa gcggatgatt gagaaccctg aaaagctgtt
    15601 gtttggtggc gacgatccaa ttatgaagct aataggctta taaaaaaaag cggcctttgt
    15661 ggccgctttt ttttctactt gaactcacca tcctcaaagg caagtttcat aatcttgtaa
    15721 acccttcctt cgctatcctt tacagggata taggtttcaa ggaagtggta tgttttgcct
    15781 ttccaggtaa ttgtcgattt aacctttttg ctaataccgg ctttaaggtt atcccagaat
    15841 gttccatact ccttcttttg tacatcggaa agctcaaggt tatcgctatg gtggctacca
    15901 attagctgtt cacggggaac cccaacaaac tggataaagg aatcgtttac attcagaata
    15961 ttgccgttaa ggtcgtactc aataacgtat gcagagcttt gaattgattt gataaactcc
    16021 tcaatttcag ccgatttgcg ttcaacttcc tcctgggtgg cctgtagctc ttccatattc
    16081 tggcgcattt cctcttcctg agctttcatc tcttcggctt gcatttgcga ttgctccagt
    16141 aacatcttag tacgtgcatt tattttaacc gaaaggatgg ttgaggcaat gctttctgca
    16201 atcttttcaa taaattcaat ttcgtgcttt tctagcacct tgaacgaggc catttcaatc
    16261 acgcctagaa cttcatcttc atgtttaagg ggtacaagaa tcacgcatcg gggatttgcg
    16321 tcgcccaaac ctgatgtgat gttaacgtaa tcgtcaggta catctgtcat gaagatggtt
    16381 tccttttcca tggcgcaggc gccaacaagc ccttcgcctt tctcaatttg tttggttaaa
    16441 tactttttcc tatcccatgc aaaagctgaa accagctcaa agtattggtt ggttttgtcc
    16501 tcatcgttcc aaaggaaaat tcccccctgg ttagctccaa catactttac caatgcggca
    16561 attacgctat cgcaaagttt ttgcaggtcg ttgttatttt ggcggagtat ctcaccaaat
    16621 tttgcgtaac cctcatttgc ccaggatcgt ttttgatcgt caaccttacg cttctcttca
    16681 tcctcagctg cctttttaag gctgtcgcgc atgtgtatta acgattttcc aagcacatcc
    16741 ttatcgctga gtagctctag gtaggttgtg tagtttcctt tacctatctc gttggcaaag
    16801 gttgttttat gaccaatacc atcaattgtg gtgttaagcg ctaacgccat ctcttcaatt
    16861 tcatcaccag tattgaggtc aacctttagc tcatccgata cttcgcccct ggccattcgt
    16921 ttcaggatat cggtaattcg ctttatgggt ttagttaggt tgtcggacac aaaaagcaat
    16981 accagcacca ttacaagtaa gcctgcaatg ctaacaaata gcgaaacgta aagggtttcc
    17041 ttggcattcg ccattattac atcaacaggt accgataggg ctaatgacca tggagttttt
    17101 gttttgccag caattattgg tgaatggcaa ataagtagtt gtttgcctgt ttcgtcggtt
    17161 tttgtgtagt agaattcttc gcccttggct attctttctg caatcttttc tttttcgtta
    17221 tcaattttga atgcttcatc aattttttta ttgataaagt ccttgctggg atgtgcggca
    17281 attattcctt cgtttgaaat taggtaagca aagcttcctt ttaccggctt aattttcgat
    17341 accatctcct ggagactttc caggctaaca tcactggcaa ctatcccgct gaaaacgttg
    17401 ttgatgtaaa tgggggagta gtaggtaatc atcagtttaa tatcggcttt tcccttagta
    17461 acctggtcaa cgtagggttc ccaaattgct tcctgcccca gctttttgta tttaccataa
    17521 ataggaggat cgccatttat gctacgctca tcgtatagcc attttatctc gtttccttca
    17581 cgccaagtgg tgataacaaa ccttccgtaa tccttatcgt aacccggcac aaacgatttg
    17641 tactcccagc tatcccatat tgagtaaatt tcagggtttt cccttaaaac agggatgtac
    17701 atgtcaagaa aaagcttttt ccactgttca tgtggcatat ttttatacac tgtaaaggcc
    17761 tgagaaaggg ttctgacaag cgataactcc ttttcaaata ttgctcctac ctcttttgcc
    17821 gatacttttg cggttagttg ggttttttca atggcatcgc tataaatatt tgccctcgac
    17881 tgccaaagga tgtaacctac ggaggcaacg taaagaattg cagttaccgt taggatgtag
    17941 agtaaaattt tctgccttat ctttagctta cgtttcatgg gacttaaatt tttttgataa
    18001 acatatatct tttttgataa aaatacaatt tctaagttta aaaaacaggg gtgttaagct
    18061 ttgaatttta ttattagtca aaatcaaaac ctaaatcaac aaaagttatg aaacaaatac
    18121 tgaaaaaaat tcagttactg ctctttgcag ttacggtttt tactgctaac gcccttgcac
    18181 agcagtataa gtacgaaact gttccaaacg acccaatggg tgtaagaatt tatacccttg
    18241 ataacgggtt aaaggtgtta atgtcagtaa ccaaggatga gccccgcatc caaacattcg
    18301 ttgccgttag agtggggagt aagaacgatc ccaaggaaac cacgggttta gctcattact
    18361 ttgagcatat gatgttcaag ggaactcccg attttggtac gctcaactgg gaaaaagaga
    18421 aagagatgat caataccatt gagcaattgt ttgaggttta ccgtaacgag accgattcgc
    18481 tcaagcgtgc tgcgatttac cataaaattg atagtatctc ctttgaagca tcaaagcttg
    18541 ccattccaaa tgagtacgac aagcttatga gtgccattgg ctcaaggggg acaaatgctg
    18601 caacatcaaa cgactacacc atttatattg agaatatccc aagcaatcag cttgaaaatt
    18661 gggcaatgat tcaggctaac cgtttcagcc atccggtgct caggcttttc cacactgaac
    18721 ttgaaactgt ttacgaggaa aaaaacatgt cgttaaccaa cgatgcccgt aaagctggtg
    18781 aggctctgtt gcgtggtcta ttccccaatc acccctatgg gcagcaaact acccttggtg
    18841 aggctgagca cctcaagaat ccctcaatga aaaatatccg tgagttcttt gacaagtatt
    18901 atgttcccaa taacatggca gttatccttg ccggcgattt taaccccgac gaggcaataa
    18961 aggtagtgga taagtacttt ggaaccctta aggcaaaacc cttgcctgag tttagtattg
    19021 ttcccgagga gccaattatt caacccaagg aatatgaggt tgttggcttg gaggctgaga
    19081 acgtatccat tggattccgt tttgccggtg caacctcagc cgatgccgat atgctatcgc
    19141 ttattaactc cattctttat aacactaaag cgggtcttat cgatttaaat atcaataaaa
    19201 aacagcttgc acttagggct tatgcatcag caagagtgat gactgattat tcaattctac
    19261 agcttggtgg ccgaaataag aagggacagt cactggaaga ggtaaaggat ctactattaa
    19321 agcaggttga gttattaaaa aagggagaat ttcctgactg gatgcttgag gcagcaatca
    19381 acaactataa gttgaacagg atgaacgctc ttgaatcagc atcgagccgt gcccggttga
    19441 tgatgaatgc tttccttaat ggtattgaat gggaaaatgt tgttaatagc attgaacggt
    19501 tacaaaaaat tagcaaggat gatgttgtaa aatttgccaa cgattaccta gggaataact
    19561 atgttgtggt ttataagcgt cagggtaaac cccaggatgt ggcaaaggtt gctaagcctc
    19621 caattactcc aattcatatt aaccgcgatg ttgaatccga ttttctaaag aaagtaaagg
    19681 aaactaaggt aacacctgtt gaaccagtgt ttattgacta taataaggat tttaccaaaa
    19741 ttaagattaa taaaaatatc gaagtccttt acaaacagaa cactgagaat gggacattta
    19801 acctggttta ctactatccg tttggtagtt taagtaatcc ggcactgacc catgcagctg
    19861 attacataca gctcctggga acctcaaagt tgagtgcgga ggaggttagc caggagtttt
    19921 atcgtttggc atgcaccttc aacattaatg ttaacgatga agatacttac attactcttt
    19981 ctggtttaag cgaaaatgcc gccaaggcct taaacctaat ggaatcacta cttaacgatt
    20041 gtcaacccga cgagtcagcc ctaaaggctt atattgccaa ccagataaaa gcccgtaacg
    20101 atgctaaacg taaccaatcg gcagtattta gtgcattggt tagctacgct acctatggag
    20161 ataacaatcc ccaaaaattt atactgagcg agaaagaact taatgagcta aaagccaacg
    20221 atctaattgg tataattaag agtttaaatt cctaccgcca tacagtgctt tactatggcc
    20281 cattagcatc gaaagattta aaggttatgc ttagcaagga gcaccgttta cccaaaaaat
    20341 tcattccaac accggaaaat aaaagatttg aaccccttga aacaacatcc gatagagtgc
    20401 tttttgccca ttacgatgca aagcaatcgt acctacaaac tttaagcagg ggtcaaaagt
    20461 acgacgataa cctttatccc attattactt tgtacaacga gtattttggt ggaggcatga
    20521 atgccattgt tttccaagaa atgcgcgaaa agcgtggcct agcctacacg gcccgctcaa
    20581 ggtacaccat tccctcaaga cctgacgatt acttcataaa catgagttac attgcaactc
    20641 aaaacgacaa ggtagttgat gccttcaacg cctttaacga actgttcaac gctatgccgg
    20701 aaaatacaat aggcttcaat ctttccaagg aggctttgat ttccgatatc cgaaacaacc
    20761 ggataagaaa catgcgagta ctttacagct acctgaatgc caaacaaatg ggttggaacg
    20821 aagatattcg taagaaactg tattcacaaa tacctactta tacctttgac gatgttaaga
    20881 aattccagga acaattcgta aaagggaaac caaaaactta cattatttta ggcaaagagg
    20941 ccgacatgaa tttccaggaa ctggaaaaac tttatgggcc tgtaactaag gtaacattgg
    21001 agcaaatatt cggttactaa tatttaaagg ccaatcgaaa aaaatggttg gcctttttta
    21061 aatcattttg atatgggaaa aaaaattctt agctcaattt tattgctcac gggtataacc
    21121 ttaaccctgt tttctcagga tagaatgact gggttaaact ttgcaacacg ttccgaggtt
    21181 atagcccgta atggtatggc ttgtacaagc cagcccttgg caacacaggt tgccattgat
    21241 attctgaagc aggggggcac tgctgttgat gctgccattg cagccaatgc tatgctagga
    21301 ttggtggaac ctactggcag tggaattggt ggcgatttat ttgccattgt atgggatgca
    21361 aaaacaaaaa aactttatgg ccttaatgcc agtggccgtt caccaatgtc gctaaccctt
    21421 gaatacttta aaaagaatgg aatcgctcac attccagctt taggaccgct tccggtatcg
    21481 gttcccggct gtgtggatgg ctggtttgag ctacataaga ggtttggccg gttgcctatg
    21541 caaaaaattc tatctcctgc catttcatac gcccgcgagg gttttccggt ttccgaactt
    21601 attgcatact actggcagcg caatgcagtg cgcttaaaac aatacgaggg ttttgctgat
    21661 gtttttatgc ccaacggcaa agccccttcc aaaggtgaaa tatttcgaaa cccttaccta
    21721 gcaaatactt atgaactgat tgcactaaag ggacgagatg agttttacaa aggctcaata
    21781 gcaaaagcca ttgatgaata catgaaacga aatggtggtt ttctgagtta cgaggatttg
    21841 gcaactcata aatcggagtg ggttgagcct gtgtcatcgt cgtatagggg ttacgatgtg
    21901 tgggaattac ctcccaatgg ccagggtata gctactctac aaattctaaa catactggaa
    21961 cagttcgata taaaatcgat ggggtttggc agcgcagaat acatgcatct gttcattgag
    22021 gctaaaaaac ttgcctttga ggatagggct cgatactatg ctgaccctga ttttgttaaa
    22081 atcccttatg aaatattgat ttcaaaagag tatgcaaggg aaagggcaaa gctaatcgat
    22141 ttgaaccgtg ctgccagaac ctacgatgcg ggcatgttac aaacaagcaa tacagtttat
    22201 ttgactgttg ccgataggga gggaaatatg gtttcgctca ttcagagtaa ttaccgtgga
    22261 atgggttccg gaatgacacc aactggttta ggttttgtac ttcaggatag gggagaactt
    22321 ttcagtttgg aggagggaca cccaaatgtt tatgctcccg gcaaaaggcc gtttcatacc
    22381 attatacctg gcttcattac caaggatggt aaaccatggg ttagttttgg cgtgatgggt
    22441 ggcggaatgc aaccccaagg gcatgcccag attattgtaa atttaataga ctttggaatg
    22501 aatttacagg aagcaggaga cgccccacga atacagcacg agggcagttc agagcccact
    22561 ggcgaacgaa tgaccgatgg aggaatggtt taccttgaat ccgggttcga ttaccaggaa
    22621 attagaaaat tattggccaa gggacacaga attgggttta gcttggatgg ttacggggga
    22681 taccaggcaa ttatgttcga tgaaaaaaat ggggtttata ttggagcctc tgaatcccga
    22741 aaggatgggc aagcggcagg gtactaaaaa tcgatattga taaaagatta ggggttgaca
    22801 tttgtgtcaa ccctttctgt ttcatccccg ttttatccca gttcatccta tcttatttgt
    22861 aatggggtat tccaaatatc aactttgcat cgtaatgtta atactaaata aaagatgaaa
    22921 aagttatttt taattacttt tgtgctaatt ggtttcgagg ctttctcaac taatatctta
    22981 acacaaacag ttagaggtgt tgtaatagat aagaacacca aaatgacatt gcctggtgca
    23041 aatgtaattc tactagattc tgatccttta agaggagtta tgaccgatga gcaaggacgc
    23101 tttaagttcg aatctgttga aattggtcgt gtgagcctaa aagtttcttt tttagggtat
    23161 aatgatgttg tgctatcaaa ccttaacctg caggttggta aggagttggt tttaaatatc
    23221 gagatggagg agttggctgt taatgttgag gcggtggttg taactgcaaa gtccgataaa
    23281 tcaactccaa tgaataaact tgccacagta agcgcaagaa cctttactgt tgaggaaacc
    23341 gagcgttatg ctggtagtcg aaacgatgtt gccagaatgg ctgcaaacta tgcaggtgtt
    23401 atgggtgttg acgattcccg gaacgatatt attattcgtg gaaatagccc aatgggttta
    23461 ctttggcgct tagagggtgt tgatattccc aacccaaatc attggggagc aaccggaac
//
LOCUS       DAOOWJ010000033        23228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2222_length_23228_cov_7.597463, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000033 DAOOWJ010000000
VERSION     DAOOWJ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 23228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23228
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2222_length_23228_cov_7.597463"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(511..1485)
                     /locus_tag="PLW22_04920"
     CDS             complement(511..1485)
                     /locus_tag="PLW22_04920"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOU74081.1"
                     /translation="MQNWVEVISRYNYPDHLKSWWQVINSVLPYFALWVAMYHSLKVS
                     YWLTLALSLIAAGFLVRIFIIFHDCGHGSFFRAERLNRLVGIPLGLLVFTPYHRWHRD
                     HFIHHSTIGNLDKRGIGDVETLTVREYLELTKWGKFKYRIYRHPLFLFAIAPILLFLI
                     QYRIPKSYMNFKQQMYIHLSNIAIVGTIVLLMWWIGWREFILIQLPIIYFASIAGVWL
                     FYVQHQFERVVWKRSHEWDYQSVALEGSSFLKLPKVLQWFSGNIGYHHIHHLSPRIPN
                     YKLQKCHDENEMFSHIKPLSFREILRSFRLRLWDEDRKRLIPFDELQC"
     gene            complement(1687..3147)
                     /locus_tag="PLW22_04925"
     CDS             complement(1687..3147)
                     /locus_tag="PLW22_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOU74082.1"
                     /translation="MNKEIFKLEPQRVWKHFHSLTQIPRPSKSEKAVIDFVVKFGNDL
                     GLETIIDEVGNVIIRKPATPGMEDRKGVILQGHLDMVPQKNNDKVHDFEKDPIEAYVD
                     GEWVTANGTTLGADNGIGVAAAMAVLEATDLVHGPVEALFTIDEETGMTGAFGLKPGL
                     LKGDILINMDSEDEGELYVGCAGGLDANIEFNTKLESVPQGTTAYKLAITGLKGGHSG
                     MDINLGRGNSIKLLFRFLYIAEKEFGVRIASIDGGSLRNAIPREAFAVVVVPADKAAA
                     FEKAVKEYESIYKSEFALSEPTLSFTSEKTGNPSTVIDNQTQSKVIRAFFGCPNGVVR
                     MSDSIPGLVETSNNLARVFTDGEKVKGQCLLRSSVDSSKDALAQKMAAIFELAGANIN
                     FTGGYPGWKPNPNSAILKVMQDVYNKKFGKVPEIKAIHAGLECGLLGGVYPNWDMISF
                     GPTIRHPHSPDEKVNIATVQKFWDFLVETLKNIPKK"
     gene            3307..4050
                     /locus_tag="PLW22_04930"
     CDS             3307..4050
                     /locus_tag="PLW22_04930"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOU74083.1"
                     /translation="MSVVFQVEWYKRVSSTNDVCQQYATKGADEGIVVAAEFQEQGRG
                     QRGNAWESQEGQNLTFSVLLRPNFLPVAEQFYLSKAISLAVSDWLVAYVTNKPVKIKW
                     PNDIYIGDSKVCGILIENSFSSQYLESSVVGVGINLNQVSFPDDLPNPTSLLLETGSR
                     VDPRKALAELVSCIYGRYQQLTEGYRENIDNDYFNAIYRKNQLCKYYSNGEEFYATII
                     GIQPTGELVLRTAEGAIRTFAFKEVTFII"
     gene            complement(4057..4464)
                     /locus_tag="PLW22_04935"
     CDS             complement(4057..4464)
                     /locus_tag="PLW22_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HOU74084.1"
                     /translation="MTTYESKIVAISKRAEDVFRILSDFRNFSPMAKDKLDDWQATED
                     SCSFKYKGMGPLGVRIIERDEFKTIKFSGDEKSPMEFFMWIQLKEVAPYDTRMKITVK
                     AELNMMMRMMVGNKLKEGIDALADGIAAAFNAV"
     gene            4574..5518
                     /locus_tag="PLW22_04940"
     CDS             4574..5518
                     /locus_tag="PLW22_04940"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOU74085.1"
                     /translation="MRIILQPTHDPYFNIATEEYLLKHSNDDYIILYRNTPSVIVGKH
                     QNTMAELNHSFINSSKIPVIRRLSGGGTVFHDLGNVNFCFMVNGREGNLVDFKRFTTP
                     IIEFLKELGLNAYRGEKNDIRIGNYKVSGNAERVHKNRVMHHGTLLYSTNMFWLEQAI
                     KSNENSYTDRAIRSNRSRTINIAELLPHPMTIETFEIALSEFLTKYHSATPLQLDDSA
                     KRTINTLATTKYTDWNWNYGYNASYDFRVKIDSNILVLKVTSGIIEKVEIIQNCSSWI
                     HKLAKLLEGKRHDYAYLKNELGNTEYSEKIQEIIENLF"
     assembly_gap    5689..5788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5789..6236)
                     /locus_tag="PLW22_04945"
     CDS             complement(<5789..6236)
                     /locus_tag="PLW22_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74086.1"
                     /translation="MKITGMRFIALLLITFLPIFSIAQETLDTIILVSNRKLICNVKS
                     VSSSKVTILKKDAAAPEELSRKQIHKILYANGRVEKFNDLAFKMIDETNFQSVVITTN
                     PADVDGLYIYGKVESVSGKNSKNAKSAEKNARIRLQRKAAALGANYV"
     gene            complement(6303..6638)
                     /locus_tag="PLW22_04950"
     CDS             complement(6303..6638)
                     /locus_tag="PLW22_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74087.1"
                     /translation="MKPLKFLDKIAIWMLRLTFAGYLILANIGYFRSIAISDFQFYIA
                     LAVVVLAVLFIVGGFTSNQGLTVISSIGIFLLLLYKALTPWPPTLTDHFLVQIVLASA
                     ALVFASRGN"
     gene            6945..7730
                     /locus_tag="PLW22_04955"
     CDS             6945..7730
                     /locus_tag="PLW22_04955"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HOU74088.1"
                     /translation="MKGAKHILMLIVSIFLWLFVAITAFLLFLIDFVVWLLTFWWDRR
                     LKLLHKYSSVWAMFYIWINPGWRIKVSGLEKIDKSKSYVIVCNHQSAFDIVLLYRLFT
                     HFKWVAKKELAKVPVIGWNLILNNSILIDRASAVSTKKMISQGLKHLKGGSSVLIFPE
                     GTRTKDGQVKRFKEGAFLLAKQANSPILPVVIEGSKDIIPKPGIVKLRQNLTLKVLDP
                     IEVEEVLSTSVPDLTKRLNQLIMEEHKKLAPEKYMNSDNKQVK"
     gene            7829..8140
                     /gene="rplU"
                     /locus_tag="PLW22_04960"
     CDS             7829..8140
                     /gene="rplU"
                     /locus_tag="PLW22_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOU74089.1"
                     /translation="MYAIVDIAGQQFKVEKDQKIYVNLLEGEAGTEVKFNKVLLVDND
                     GKVAVGTPELKNASVTAKILGHVKGEKVIVFKKKRRKGYRVKNGHRQNYTQIQIAEIV
                     A"
     gene            8161..8421
                     /gene="rpmA"
                     /locus_tag="PLW22_04965"
     CDS             8161..8421
                     /gene="rpmA"
                     /locus_tag="PLW22_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOU74090.1"
                     /translation="MAHKKGVGSSRNGRESHSKRLGVKLFGGQVAKAGNIIVRQRGTK
                     HYPGMNVGMGKDHTLYALIDGVVTFRKKADDKSYVSVLPQES"
     gene            8510..9787
                     /gene="serS"
                     /locus_tag="PLW22_04970"
     CDS             8510..9787
                     /gene="serS"
                     /locus_tag="PLW22_04970"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOU74091.1"
                     /translation="MLTLKYIRENTEEVIRRLAVKHFDGSVIIAKIIELDDKRKAIQL
                     QLDNNLAEQNRIAKEIGKLFSEGKSAEANVAKAKSAELKELSKNLSQELKEVEEEQTK
                     LLLQVPNLPHESVPQGVSASDNVVVRSGGSIPKLHEEALPHWDLASKYNIIDFELGVK
                     ITGSGFPVYRGKGAKLQRALINFFLDYNTAAGYEEVEPPHMVNEDSAYATGQLPDKDG
                     QMYHVTADGFYMIPTAEVPVTNIYRDVILHAAMLPIKLTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPDRSYETLEEMVNHVEGLLMKLELPYRIVKLCGGDMSFTSA
                     LTYDFEVFSAAQHKWLEVSSVSNFESFQANRMMLRFKEEGDKKAQLAHTLNGSSLALP
                     RIVAALLENNQTPHGIKIPKVLQPYCGFDKIDL"
     gene            10001..11566
                     /locus_tag="PLW22_04975"
     CDS             10001..11566
                     /locus_tag="PLW22_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74092.1"
                     /translation="MKAFQKILVSIMLTFSFVAAYAQMEELPKSDTAGVTGGAVIGAV
                     TIDGKTYQQFALRTDIPIGKFGFGIDIQLLFDENGQIRKDEWDEWQDYIDKIYYIRYG
                     KKDDPFYIRLGGLDYTYLGYANIVNGYSNMIQYPSRRRYGGELSFWIPAERYKGMPVA
                     GGEFFFNDMKELFVESPRKSAMLVGSRIFYRPLRFLEFGVTGVADLNEYNAFEDRDGD
                     GVPNLIDLYPRDKNLATDMDKISLNDWNYLLNDTYWGPTREERYQKLLSLGLVPADTL
                     RMENLFNLNNQTSKMFAYSFDIGIPIARKENFKFDIYSHITQLRGENRTYGWGVAIPG
                     IRMILGTSNVQNFLTFKAEYRRSSKEFLFGYFNNTYELERAQFIGRDSVITKQQKLIA
                     IQQELNGIYASAELNMFGYIVGYAGYQDLTGSDKTHMRSLHGEVRMGETLKKMLHFAD
                     LKGYYIQNNVQDFRQWKTPSSLVGFNIGYNYKGAIVGIDYRWTFQDLDGNGIIKGSAE
                     TIKTISFRTAVTF"
     gene            complement(11608..13053)
                     /locus_tag="PLW22_04980"
     CDS             complement(11608..13053)
                     /locus_tag="PLW22_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOU74093.1"
                     /translation="MSASIQLALVTIGYLLFIIGWGIYQGRKVKNNADYSIAGRNLPG
                     WVAALSERATGESSWALLGLPGAAYAMGIAELWTAIGCVVGIITAWWLLAWRLRDEAE
                     KYSATTFSDYLAARHGEVAKWIRLVSSSTIVFFFFLYVGAQFLGGGKTLDTMFGIKPQ
                     WGMLITALIIIPYTIYGGFQSVVYTDVVQALVMIFTLTVGPIVGFFYLANHPEVYSSS
                     ITIALESASPTHASLFNGLKGAAAGMFLVGGFSWFFGYLGGMPQLTMRFMAIKDARQA
                     KLARNIGITWTVVAYIGAMLLGMIGLAIFGPNALTDREYVMPATIMKIFPPALAALLI
                     TGAIAAMISTADSLLVLSSTELSENIVKPFRRINDERLVLRQSRIITASLAIIALAIA
                     YVSPQKVIFTLVSYVWAGIGCTFSVVILLTLFWKPFHGRAAITAMILGLVFTIVWISS
                     GMDKVVTAKLVNFFFTLIVAVLSTYLIPSKK"
     gene            13176..14186
                     /gene="amrS"
                     /locus_tag="PLW22_04985"
     CDS             13176..14186
                     /gene="amrS"
                     /locus_tag="PLW22_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU74094.1"
                     /translation="MEALYYMHLENKAVQCNLCPHECKLTPGKRGICKTRYNDNGEMR
                     LLISGVIASSGLDPIEKKPLFHFYPGSKIYSIGGYGCNLRCSFCQNYEISQYVPDSNS
                     RLRENLPENIVDKALFLPQNIGIAYTYNEPTVFFELMLATARLAKGKDLKNVMISNGF
                     INHKPLLELLDVIDAFNIDLKAFTNEFYKTQAGGKLAPVLNNLKTIKKNGNHLEIAFL
                     VIPELNDSTNEAVEMFRWINENLGNDTPLHINRYYPAYKLSIPPTPPEKLVELRDVAM
                     EYLKFVYIGNVHNSDIGTKTTCPSCNTTVIERHGYSTTLKMIDTRGYCKICGYGPIAV
                     MG"
     gene            complement(14183..14755)
                     /locus_tag="PLW22_04990"
     CDS             complement(14183..14755)
                     /locus_tag="PLW22_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU74095.1"
                     /translation="MLRDLVVQNRSYRRFDQSHNIDLGLLEQLVELCRFCPSGRNLQP
                     LKFMIINTPEWCNNLFPNLAWAGYLKDWDGPVEGERPSAYIVVLEDQRLSTASQWDQG
                     IVAQTILLGAVEKGLGGCIIASVKKENLHKLLNLPEYLKVALVIALGKPVEKVVIDDI
                     DDSGSVIYWRDSEQVHHVPKRKLSELIWRG"
     gene            complement(14758..15345)
                     /locus_tag="PLW22_04995"
     CDS             complement(14758..15345)
                     /locus_tag="PLW22_04995"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOU74096.1"
                     /translation="MKHELPKLQYSLNALAEVISEKTMEFHYGKHHQAYVNNLNNLIP
                     GTKFENASLEQIVMEAEGGVFNNGAQVWNHTFYFEAFSSVPKHKPEGDLLKAIEASFG
                     SFEEFLSKFSNAAATLFGSGWAWLVVDTDGKLNITQESNAGNPLRKGLKPLLTCDVWE
                     HAYYLDYQNRRPDYIQAFWKIVDWSVVEKRFEKVR"
     gene            complement(15357..17795)
                     /locus_tag="PLW22_05000"
     CDS             complement(15357..17795)
                     /locus_tag="PLW22_05000"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74097.1"
                     /translation="MFFNRIHIQNLILSCFITVIFPVLAHGQKMMVDTAQVNSQLATV
                     RSLIDVREFSAAKKIAQKVLANTSASEYNEARAWCEYYLGIIYFEITPIDSAFYWLNR
                     AKADFSLINNQIALIKTYNRLGIFYRRTDLNDSSLHYYNLALNKATAIADSTGLAEAN
                     YGLGMVNAQIGNYTKALEHYSNSLAIREKLNDKAGIASVYNSMGILFWEQGAYSTALD
                     FFFKALPLRVETKDRQGEAFLLNNIGLIFRDVAQYEKSLDYLRKSWKIKIEINDKRGI
                     SNSLMNIGSVYLLLGQVDSALLYFEDALALKQILLDRGGVANINRFLGEAYMKIKMYD
                     KAQLYLTKAIEGYTKLEEPRGVVESKYDYALLLFQMGNSPKAFALVDEVLEQAKKHGM
                     MDMVAQSYNALYQFNLAKNNCQGALQNYEKYIAVKDSIAGTEKMKQILAAQLQVEYEN
                     IVRQHFERFNEQLSLVDSQKQSRTRLLILVAIAFVLLLGLFLGLLYTMYKRKQALVEV
                     ESQRISVDIKQRELQEQRDELERQKNLVVYQRDRIINILTDLGESIEYARKIQQAVFP
                     TDALLSQYFRDFFVLYLPKETVGGDFYWVGNCSGKIGFAVADCTGHGVPGGFMSMLGI
                     SMLNDLVANTPTSSPGLLLGKLRDNIISALRQGGHEDDSHDGMDVAFCTFDRSTHTLN
                     YSGANMPIIISTNSNIPNDNRIVQVGEGLIEFRPDRMPVAYYERMERFNELSIKLEPN
                     DTVYLFSDGFTDQFGGKPSKKFGYHSFRNLILSIKHLPLYEQKMTLYRTLEKWKGEEE
                     AQTDDILILAVKPI"
     gene            complement(17897..18508)
                     /locus_tag="PLW22_05005"
     CDS             complement(17897..18508)
                     /locus_tag="PLW22_05005"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOU74098.1"
                     /translation="MAFQLPKLKYDYKALEPYIDEMTMDIHYNKHHGAYTNNLNNAIA
                     GTSIEALPIEEILRGVSKHSPAIRNNGGGFFNHNLFWEIMSPNGGGKPNGELLKGIEK
                     FFGSFDEFKEKFSNAAATRFGSGWAWLVWTGKDLQVFSSPNQDNPLMDVSEVKGTPIL
                     GLDVWEHAYYLKYQNRRPEYIQNFWNVIDWKAVEEKYLVATGK"
     gene            complement(18586..18771)
                     /locus_tag="PLW22_05010"
     CDS             complement(18586..18771)
                     /locus_tag="PLW22_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74099.1"
                     /translation="MKRPFIGKSKHLQDTILSLDHKWYVEFWVIRYYFLGIEVYRTKK
                     EKVRYVNGKNGNSVNSQ"
     gene            complement(18917..19741)
                     /locus_tag="PLW22_05015"
     CDS             complement(18917..19741)
                     /locus_tag="PLW22_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOU74100.1"
                     /translation="MNLSLNFSTCPNDTFMFYALVHGLLSDKYTFDVYMADIEDLNLL
                     ALEAKPDITKVSYGAYPLLAQHYQLLDSGSALGKGVGPLLISKETIPFEQVKHKRIAL
                     PGEHTSAHLLFNLVFPDVHDKRFYLFSQIEDAILNNEVDAGVIIHESRFTYQFKGLTK
                     LMDLGDEWEKISGLPTPLGGIAIRRSLPDDVKHDINRLLRESVEYAFQYPENTMGFVS
                     QFAQELSTEVMRNHISLYVNDFSITLGQVGRKAIIQLISSSKPTHNFDERSVFVYE"
     gene            complement(19743..20399)
                     /locus_tag="PLW22_05020"
     CDS             complement(19743..20399)
                     /locus_tag="PLW22_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74101.1"
                     /translation="MKKILIVVASQVEILPQYRQPGNDFYSLQQYGIHADILISGPGA
                     VPTVFALSRAADAYDIIINGGIAGSFTEQLPLGNVVLIDSDSFADYGVDDNGTFRHID
                     NILPPLQKALPLRELVNPYTIKPNPKLFGVRGITVSTVSGSNERIAMIKRSWDAQIET
                     MESAAVFYACLKLGKPFFCLRAVSNYVEPRNRQNWKIDIAVQNLWNELVLLVSYLNTL
                     "
     gene            20527..20946
                     /locus_tag="PLW22_05025"
     CDS             20527..20946
                     /locus_tag="PLW22_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOU74102.1"
                     /translation="MPIAYLTRRERFSAAHRLYKQELTDEENFRLFGQCSHPNWHGHN
                     YELFVTIKGEVNQVLGYVVNLKVVSQIIKERIISKLDHKNVNIDVDFMKGVLATTENL
                     AIAIWKELEKPLSDVGAQLHCIKIKETENNFVEYYGD"
     gene            20951..21589
                     /gene="folE"
                     /locus_tag="PLW22_05030"
     CDS             20951..21589
                     /gene="folE"
                     /locus_tag="PLW22_05030"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOU74103.1"
                     /translation="MEIVNQNASFNEDEGYSKIERWNPETTEKLAYHYKEILKLIGED
                     VGREGLLQTPVRVAKAIQFLTHGYNIDPHVLVRSAKFHEEYKQMVLVKDIELYSMCEH
                     HMIPFYGKAHVAYIPNGYITGLSKVARVVDAYARRLQVQERLTVQIRDCLQETLNPLG
                     VAVVIEAAHMCMQMRGIQKQNSVTTTSAFTGAFLTHNETREEFIHLIGVKLH"
     gene            complement(21665..22408)
                     /locus_tag="PLW22_05035"
     CDS             complement(21665..22408)
                     /locus_tag="PLW22_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU74104.1"
                     /translation="MKKILVILSLVAITFNAANAQLFKVFGHDVGFIYVGPKVGMNFS
                     KFSQWSYPTHEVKSRIGYQFGIVGDFGFTNRFSFQTELNFISKGTKGIYGTVEETKRM
                     PAIQIPLLAKYTFSLLGLKRVYANGGVYSTTRIGSGKVIYEDGAVYDDYHWTRFDWGF
                     SVGCGAEYPTKDGIWGLDLRYDLGMVDIYRQMDENTKSRFRSFQFALTYKFDMVDLYF
                     KLRKKNNDEGGDRETGNKGPKGLKVEQRN"
     gene            22539..23135
                     /locus_tag="PLW22_05040"
     CDS             22539..23135
                     /locus_tag="PLW22_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74105.1"
                     /translation="MGILEIEKDIHARIVRSVNAQLGNYITFNKNCVIEPIEQTFFPN
                     STLLSIIDFDCIPASKLMVIDNGVQVYPLSGLGQWNSILMNERLNLNIKNVLDYLKLY
                     YSTHVFPYQYQKIVETIHDLDFIEFPEIKLFEVINQRIQAPVVTLSNKQFTIYCNITS
                     NRTLYNAAFDVYPDGKVLLLDKKIVMQDLPIGEMVIDL"
BASE COUNT         7322 a         4827 c         4599 g         6380 t
ORIGIN      
        1 gcgctcccct ccgaaagcgg gtgcaaaagt agaaaagcgt ttttaattac gcaagaggat
       61 tatcaaaaaa aacaagattt ttttaaaaaa agttacatat tgttgactat caatgtcaat
      121 ttatacaaga aaaattaaga acgtgatgtt aaatcaaccc aagtgcccat ggattaagaa
      181 attcagcaag gctaatttct agaggaatga gtcggaatga gtcggaatag acggaatatg
      241 atggagttag aggaagttag aggaagttca attccgtgaa acgtgaaccg tgaaacgtga
      301 accggaagaa ttaacaatat agctgtttta gaggatgtta gaggatattc aaatccgtta
      361 aatgtgaacc gtgaaccgga agaattaaga ctatgacaga gctagaggaa gttagaggga
      421 aaaaaaagtt aaatcaaaaa ccttagatta acaaatttaa ccgcccaaaa ataggaatac
      481 agagacattt ttagagaaat ttcgaactac tcaacattga agttcgtcaa atgggataag
      541 tcttttcctg tcctcatccc ataacctcaa cctgaaagat cttaatatct ccctaaatga
      601 gagaggctta atatgtgaaa acatctcgtt ctcatcgtgg catttctgaa gcttatagtt
      661 aggaatcctt gggcttaggt gatgaatatg atgataccca atgttaccgc taaaccattg
      721 taaaaccttg gggagcttaa ggaatgaact tccctctaga gcaaccgatt gatagtccca
      781 ttcatgggag cgcttccata ctaccctttc aaattgatgt tgaacgtaaa aaagccaaac
      841 acctgcaatt gatgcaaaat agattatggg tagctgtatc aaaataaatt cacgccaacc
      901 tatccaccac atcagtaata caattgtacc aacgatggcg atattggaaa ggtgaatata
      961 catctgttgc ttaaagttca tgtacgactt tggtattctg tactgaataa gaaatagaag
     1021 aattggggca attgcaaaaa ggaaaagcgg atgcctataa attctgtact taaatttacc
     1081 ccactttgta agttcaaggt actccctaac cgttagggtt tcaacatcgc caataccacg
     1141 tttatcgagg ttgccaatgg tagagtgatg aataaagtga tcgcgatgcc agcgatggta
     1201 aggagtaaat accaataacc caaggggtat tcccactaat cgatttaacc tttcagctct
     1261 aaaaaaggaa ccgtgcccac aatcgtgaaa aatgatgaaa attcgaacaa gaaagcctgc
     1321 tgcaatgagg gagagagcta gggtcaacca gtatgatact ttcaaactat ggtacatagc
     1381 cacccaaagc gcaaaatagg gcagcacgga atttattacc tgccaccagc tttttagatg
     1441 gtcgggatag ttgtaccttg aaataacttc aacccagttt tgcattttcc agaatagtaa
     1501 ttcagtataa taatttcaat aaaactaact cctaaatata caagggttat gaaagaaatt
     1561 caagacttac gatacaaaag agcaaggatt tgtaacaaat attaacagtg gataaatatt
     1621 ctcagaaccg atagattaac taataaaaaa ggggctttta gccccttttt attgaaacca
     1681 tactggttat tttttgggga tattcttgag tgtttcaacc aaaaagtccc aaaacttttg
     1741 aacggttgca atgtttacct tctcatcggg tgagtggggg tgacggatgg ttggcccaaa
     1801 cgaaatcata tcccagttag ggtaaacacc acctagcaag ccgcattcta acccggcatg
     1861 gattgcctta atctcgggca ctttgccaaa ttttttattg taaacatcct gcattacctt
     1921 gagtatggct gagtttggat taggtttcca tccagggtaa cccccggtaa aattaatgtt
     1981 agcaccggcg agttcaaaaa tagcagccat tttttgagct aatgcatcct ttgaggaatc
     2041 aactgagctg cgcagcaagc attggccttt tactttttcg ccatcggtga aaacgcgtgc
     2101 aaggttgttg gaggtttcaa ccaaacctgg gattgagtcg ctcattcgaa caacgccatt
     2161 ggggcagcca aaaaaggctc tgattacctt tgattgtgtt tggttgtcaa taactgttga
     2221 aggattccct gttttttccg aggtgaacga aagcgttggt tcacttaaag caaactctga
     2281 tttataaata ctttcatact ctttaacggc tttttcaaat gcagcagctt tatcagcagg
     2341 aaccacaaca acggcaaatg cttcgcgtgg gattgcatta cgcaagctcc cgccatcgat
     2401 tgaggcaatg cgaactccga attccttctc ggcaatgtat aggaagcgga aaaggagttt
     2461 aatggagtta ccacgaccaa ggttaatatc cataccggag tgtccaccct ttagtcctgt
     2521 aatggcaagc ttataggcag tggtaccttg gggtacagac tcaagtttgg tattaaactc
     2581 aatgttggca tccagtccac cagcacaacc cacatagagt tcaccttcgt cctcggagtc
     2641 catgttgata aggatatcac ccttaagcag gccaggtttt aacccaaaag ccccggtcat
     2701 tccagtctcc tcgtcaatgg tgaaaagggc ttcaacaggg ccatgtacta agtcagttgc
     2761 ttccaggaca gccatagcag ccgccacacc tataccgttg tcggctccaa gagttgtacc
     2821 gttggctgtt acccattccc catcaacata agcttcaatt gggtcctttt caaaatcgtg
     2881 aactttatcg ttattctttt ggggtaccat atcgaggtgg ccctgaagaa taacaccttt
     2941 acgatcctcc atgcccggag tagccggctt acggataata acattcccta cttcatcaat
     3001 aatggtttcg agcccaagat cattcccaaa ttttactaca aaatcaatta cagccttctc
     3061 gctttttgat gggcgaggaa tttgcgttaa gctatggaaa tgtttccaaa cacgctgtgg
     3121 ttcaagtttg aagatttctt tattcatatc aacctaattg tttaaatttt taatttgatt
     3181 ggccttaaag ataccaatta ttaaccaaca taacaaactg ctgttttaaa acatcaatct
     3241 atgcccccaa acatgcaact ataccaaaaa atcaacgtaa taaaacgggc ataaaaaata
     3301 attgacgtgt cagtagtttt tcaagttgaa tggtataaac gcgtttcgtc cacaaacgat
     3361 gtttgtcaac agtatgctac caaaggtgca gatgaaggaa tagttgtagc ggctgaattt
     3421 caggagcaag ggcgaggaca gcgaggtaac gcatgggaaa gccaagaggg gcaaaacctc
     3481 acctttagcg ttttacttcg cccaaatttc ctacccgttg ctgaacaatt ctacctttca
     3541 aaagcaatat cgcttgcagt ttccgattgg cttgtagcct atgtgacaaa caaaccagta
     3601 aaaattaaat ggccaaacga tatatatata ggtgatagta aagtttgcgg aatactcatt
     3661 gagaatagct ttagtagtca gtacctggaa agttcagttg ttggtgtggg tataaattta
     3721 aaccaggttt ctttccccga cgatttgcca aatccaactt ctttactact tgaaaccgga
     3781 agcagggttg atccccgtaa agcattagcc gaattggtta gctgcattta tggacggtac
     3841 caacagctta ctgaaggcta cagagaaaac atcgataatg actactttaa tgccatttac
     3901 agaaaaaatc agctttgcaa gtattactcc aatggcgagg agttttatgc tacaataata
     3961 ggcattcagc ccactgggga gttagtccta aggactgcgg aaggtgcaat taggacattt
     4021 gcatttaagg aagtaacctt tataatatag gttaagctat accgcattaa aagcggcagc
     4081 aatgccatcg gcaagggcat cgattccttc cttaagttta ttgccaacca tcatgcgcat
     4141 catcatgtta agttctgcct taacagtaat tttcatacgg gtatcgtaag gtgctacctc
     4201 cttaagctga atccacatga agaattccat aggtgatttt tcatcaccag aaaattttat
     4261 agttttaaac tcatcgcgct caatgattct gacacccaaa ggccccatcc ccttgtactt
     4321 gaagctacag ctatcctcag tggcttgcca gtcatcaagc ttatccttgg ccattggcga
     4381 gaaattcctg aaatcagaaa gaatcctaaa aacatcctcg gcccttttac taattgctac
     4441 aatcttactc tcgtaggtag tcattttgtt aatttttggt ttgcaaaaat agtaaactgc
     4501 cgtttaaaaa gaaccaattc aaatttttgt agtttatatt tatattgaat aatttacagg
     4561 ttttaagttt aaaatgagaa taatactaca acctacacac gacccatact ttaacattgc
     4621 tacagaggaa tacctcttaa agcatagcaa tgacgattat attattttat accgtaatac
     4681 cccgtcggtt attgttggaa aacaccagaa tacaatggcc gaacttaacc acagttttat
     4741 caacagtagt aaaattcccg taattcggag gttgtcgggt ggagggacag tatttcacga
     4801 tttagggaat gttaacttct gcttcatggt aaacggccga gagggtaatc tggttgattt
     4861 taaacgcttt acaacaccga ttattgaatt tctgaaagag cttgggttaa atgcctaccg
     4921 cggcgaaaag aatgatatta ggattggcaa ctataaggtt tccggcaatg ccgaacgtgt
     4981 gcataaaaac agggtaatgc accatggaac cctcctgtat agtaccaaca tgttttggct
     5041 ggagcaagcc ataaagtcca atgagaacag ctacaccgac agggcaatac gatcgaaccg
     5101 aagccgaaca ataaacatag cggagcttct tccccatcct atgactattg aaacttttga
     5161 aatcgcccta tcagaatttc ttactaaata ccactctgcc actcccttac aactggacga
     5221 cagcgcaaag agaactataa acacactcgc caccacaaag tataccgatt ggaactggaa
     5281 ctacggctac aatgcaagct atgatttcag ggtaaaaatc gactcaaata ttcttgtttt
     5341 aaaggtaact agtgggatta ttgagaaagt tgaaataatt caaaattgca gtagttggat
     5401 acataagctg gcaaaactac tggaagggaa acgacacgat tacgcttacc tcaagaatga
     5461 attaggtaat actgaatact ctgaaaagat tcaagaaata attgagaatc tattctagcg
     5521 ctagtggggt gtttggtttt cgttcttagg tggctcaaag ccgtaggcaa ccccctcgta
     5581 atagtgagtt ggaacctctt tataaccgcc tctactctcg cttttggtaa ccagaacata
     5641 gtttgcccca agggcagccg cttttcgctg caagcggata cgggcattnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga acatagtttg ccccaagggc agccgctttt
     5821 cgctgcaagc ggatacgggc attcttttca gccgatttgg catttttgct atttttcccc
     5881 gatacagatt ctactttccc atatatatag agaccatcta cgtcggcagg attagtagtt
     5941 atcactaccg attgaaaatt ggtctcatct atcatcttaa aggccaaatc gttaaatttt
     6001 tcgactctgc cattggcata aagtattttg tggatttgtt tgcgtgaaag ctcttcaggt
     6061 gcagcagcat cttttttaag gatcgttact ttacttgaac taaccgattt tacattgcaa
     6121 ataagttttc ggttacttac cagtataatt gtatccaagg tttcctgtgc tatggagaag
     6181 attggaagaa aagttatgag gagtaatgct ataaatcgca tgcccgtaat tttcatttta
     6241 atgaattgac tgcaaagtta atgccatttg atgttataac aaaaacaaac cattaaaata
     6301 tatcagtttc cacggctggc aaatactagt gccgccgaag ccagtactat ctggactaaa
     6361 aagtgatcgg tgagggttgg aggccaagga gttagtgctt tatacagtag tagtaaaaag
     6421 ataccgatag atgatatcac tgttaaacct tgatttgagg tgaaacctcc tacaatgaac
     6481 aacacagcta aaaccacaac tgctaatgca atatagaatt ggaaatcgga aatagctatg
     6541 cttctgaaat atccgatgtt tgccaaaata agataccctg caaaggttag ccttaacatc
     6601 caaatggcta tcttgtcaag aaattttaag ggtttcatat accagtttat taatctgtta
     6661 gtaataacgt tttacaaatg tattaataat taacatacta aataccatgc aaaaagaatt
     6721 attcagttca aactccatgt aggagtaaca aaaccatttg catattgaac aatgcttaac
     6781 ctctcaatgt ggaaattctt aactcacaca ctgaaacgta tggcataggt tctaaaataa
     6841 cttaaacggc atagatctca aattagtata tcaacaaata ttgtaagaaa accccacgat
     6901 caattcctgc aaacaattgc tatttttgca ggaaaattat tgctatgaaa ggagccaaac
     6961 atattctgat gctaattgta tccatttttt tatggttatt tgtggccatt acggcattcc
     7021 tactctttct aattgatttt gtagtatggc tcttaacttt ctggtgggat aggcgtttaa
     7081 aactactgca taaatattcg tcggtttggg cgatgtttta tatttggatt aacccgggtt
     7141 ggagaatcaa agtttcaggg ttggagaaaa ttgacaaaag caaatcctat gttatcgtgt
     7201 gcaaccacca aagcgccttc gatattgtgc tgctttacag acttttcacc catttcaagt
     7261 gggtggccaa aaaggagttg gccaaggttc cagttattgg gtggaacctt attcttaaca
     7321 atagcatact aattgacagg gccagtgcag tgagcactaa aaagatgatt tctcaggggc
     7381 taaaacacct aaaaggaggg agttccgtac taatatttcc tgaaggtaca agaacaaagg
     7441 atggtcaggt taagcgattt aaggaagggg catttctgtt ggctaagcaa gcaaactcgc
     7501 ccattctacc tgtggttatt gaaggttcaa aggatattat cccaaaacca ggaattgtta
     7561 agctacgcca gaacttaaca ttaaaggttc ttgaccctat tgaagttgaa gaggttttat
     7621 caacttcagt acctgatttg acaaagcgcc tgaaccagtt aattatggaa gagcataaaa
     7681 agttagcccc cgaaaagtac atgaattccg ataacaaaca agttaaatga taaaaacaca
     7741 aaaaagtttt actgattatt tgacaattaa ttaaactttt atacctttgc aagcccaaaa
     7801 tattgtcaaa caattcatac taaatacgat gtacgctatt gtagatattg caggacagca
     7861 atttaaagta gaaaaagacc aaaagatcta cgtgaacctg cttgagggag aggcaggaac
     7921 agaggttaag ttcaacaagg tgctgcttgt tgacaacgat ggtaaagtag ccgtgggaac
     7981 tcctgaacta aaaaacgctt cggttactgc taaaatcctt ggccatgtaa aaggcgaaaa
     8041 ggttattgta ttcaagaaga aacgtcgtaa agggtatagg gtgaaaaatg gacaccgcca
     8101 aaactatact cagattcaga ttgcagaaat agtagcataa cacgaaaata ttgattagtc
     8161 atggcacaca aaaaaggagt tggtagttcc cgtaacggac gcgaatcgca cagcaaacgc
     8221 ttaggcgtta agctttttgg tggtcaggtt gccaaagctg gcaacattat tgtacgccag
     8281 agaggaacta agcactatcc cggtatgaat gttggcatgg gcaaggatca caccctttac
     8341 gccttaattg acggtgtggt aactttccgc aaaaaagccg acgacaaatc gtacgtatca
     8401 gttttaccac aagaatcgta gttttactaa aattttcata actttggtct cctgtgcaat
     8461 gccttcaagt attgaacagg agattttgtt ttattaaaaa cccaacacta tgcttaccct
     8521 aaagtatatc agagaaaata ccgaggaagt tatccgtagg ttagcagtaa agcattttga
     8581 tggttctgta attattgcca aaattataga acttgacgac aagcgtaagg ctattcagct
     8641 tcagcttgac aataaccttg ctgagcaaaa ccggattgca aaggaaattg gaaaactttt
     8701 ttccgagggg aagtcagctg aggcaaacgt agccaaggca aaatcggctg agcttaagga
     8761 actgtccaaa aacctctcgc aggaacttaa agaggttgag gaagagcaaa ctaaacttct
     8821 gctccaggtg cctaacctgc cccatgaatc agttccgcag ggtgtaagcg caagcgacaa
     8881 cgttgtagtt cgatctggag ggagtattcc aaaactacac gaggaggcct tgccacactg
     8941 ggacctggct tcaaagtaca atatcatcga ttttgaactt ggcgtcaaaa taaccggtag
     9001 tggtttccca gtatatagag gtaagggtgc aaagctccaa agagccttga ttaacttttt
     9061 ccttgactac aatactgcag ccggatacga ggaggtagaa ccaccgcaca tggtgaacga
     9121 ggactctgct tacgcaaccg gacagctccc cgataaggat gggcaaatgt accatgtaac
     9181 cgctgacgga ttttacatga tacctactgc ggaggttcca gtaacaaaca tttaccgcga
     9241 tgtaatttta catgcagcga tgctccccat taagctaacc gcttacactc catgcttccg
     9301 ccgcgaggca ggttcgtacg gaaaggatgt tcgtggttta aacaggttac accagttcga
     9361 taaggtagaa attgttcaga tacagcaccc cgaccgttca tacgaaaccc ttgaagaaat
     9421 ggttaaccac gttgaggggc tacttatgaa actggaactc ccctaccgaa ttgttaagct
     9481 ttgcggtggc gatatgagtt tcacttcagc gctaacctat gactttgagg ttttttcggc
     9541 agcccagcac aagtggcttg aggttagctc agtatcgaac tttgaatcgt ttcaggcaaa
     9601 tcgcatgatg cttcgcttta aggaggaggg cgacaagaaa gcacagctgg ctcataccct
     9661 taacggaagt tcgcttgcat tgccccgtat tgtggcagcg ctacttgaga acaatcaaac
     9721 cccacatggt attaagattc ccaaagttct tcaaccctac tgcggatttg acaagattga
     9781 tttgtaaagt acgaattgca ataaaatcag tccccttgag aactttttca aggggatttt
     9841 tgttagtggg aataaaatga agactggcaa aggagaaatt gcattcccat gaaaaaaagt
     9901 ttggcctgtt atttgtgtta aatggtttta aaataaattt gaaacaatta atattaccct
     9961 tttggcactg aaaaaaatgt ttaaccaaac actattagca atgaaagcat tccaaaaaat
    10021 cctggtaagc attatgctta cgttttcatt tgtagcggct tacgcacaaa tggaggaact
    10081 ccccaagagc gacacagcag gtgttactgg tggagccgtt ataggagcgg ttaccattga
    10141 tggtaaaacc tatcagcaat ttgcgctccg taccgatatc ccaataggta agttcggttt
    10201 cggcatcgat atccagctac tttttgacga aaacggacaa atcaggaaag atgaatggga
    10261 tgaatggcag gactatatcg ataaaattta ctacatccga tatggtaaaa aggacgatcc
    10321 gttttacatc cgtttgggcg gattggatta cacctacttg ggttacgcca acattgtgaa
    10381 tggctactcc aacatgattc agtatccatc gcgcaggcgt tatggtggcg aactgtcgtt
    10441 ctggattccg gctgagcgtt acaagggaat gccggttgca ggtggtgaat ttttctttaa
    10501 cgacatgaaa gaactttttg ttgaatcgcc ccgcaagtcg gcaatgctgg tgggtagccg
    10561 tattttctac cgcccgctac gcttccttga gtttggggtt actggcgttg cagaccttaa
    10621 cgagtacaac gcatttgaag atagagacgg cgatggtgtt ccaaacctga ttgaccttta
    10681 ccccagggat aaaaacctgg caacggatat ggataaaata tccctaaacg attggaatta
    10741 cctgcttaac gacacatact ggggaccaac ccgggaagag agataccaaa aacttctgag
    10801 tcttggcctg gtacccgccg ataccctgag gatggaaaac cttttcaacc tgaataacca
    10861 aacttcaaaa atgttcgcct acagcttcga tattggaatt cctattgccc gcaaggaaaa
    10921 ctttaagttt gatatttact cgcatataac ccagctacga ggggagaaca gaacctacgg
    10981 gtggggcgta gctatacccg gtattaggat gattcttgga accagcaatg ttcagaactt
    11041 cttaaccttc aaggctgaat accgtcgttc atccaaagag ttcctgtttg gatacttcaa
    11101 taacacctat gagctggaaa gagcacagtt cattggaagg gatagcgtaa taaccaagca
    11161 acagaaatta atagctatcc agcaggaact taatggtatt tatgccagtg ctgaactgaa
    11221 catgtttgga tatattgtag gttatgcagg ctaccaggat cttactggat ctgacaaaac
    11281 ccacatgcgt tcacttcacg gtgaggttcg tatgggcgaa accctcaaga aaatgcttca
    11341 ctttgctgac ctaaaaggat actatataca gaataacgtt caagatttca ggcaatggaa
    11401 aaccccaagt tcacttgtgg gctttaatat tggctacaac tacaaaggag ccattgttgg
    11461 aattgactat cgatggacat tccaagacct tgacggaaat ggaatcatta aggggagcgc
    11521 tgaaaccatc aaaacaatca gctttagaac tgctgtaacg ttctaatcaa taaaacccca
    11581 aaaaagggag ctgttcagct ccctttttta tttctttgat ggaattaaat aggttgataa
    11641 aacagccact atcaatgtaa agaagaaatt aacaagtttt gctgtaacca ccttatccat
    11701 tccagagcta atccatacaa tagtaaaaac taaacccaaa atcatagccg ttattgctgc
    11761 cctcccatga aagggtttcc agaaaagggt aagaagtatt actacagaaa aggtacaacc
    11821 aatacccgcc cacacgtagc ttaccagggt gaatatcact ttctggggcg atacataggc
    11881 tattgccaaa gcaattatgg caagcgaagc ggtaataatt cgtgattggc gtaaaacgag
    11941 cctttcgtca ttaatacgcc tgaatggttt aacaatattc tcgcttagct cagttgatga
    12001 tagcacaagc aatgaatcgg cagtagaaat cattgcagca atagcccctg ttataagtaa
    12061 ggctgcaagt gccggaggga atattttcat gattgtagca ggcatcacat actccctgtc
    12121 ggtaagggcg ttggggccaa aaatggctaa accaatcatt ccgagcaaca tagcgccaat
    12181 gtaggcaaca actgtccagg taatgccaat attacgcgca agcttagcct gcctggcgtc
    12241 cttaatggcc atgaacctca tggttagctg gggcattcca cctaggtaac caaagaacca
    12301 gctaaagcca cccacaagaa acatacctgc ggctgctccc tttaaaccat tgaataacga
    12361 agcatgggtt ggactcgcgc tctccagagc aatggtaatg gagcttgagt atacctcagg
    12421 atggtttgcc aggtagaaaa acccgacaat tgggccaacc gttaaggtga aaatcattac
    12481 taaagcttga accacatcgg tgtatacaac gctctgaaat cctccgtaaa tggtataagg
    12541 gataatgatt agtgcggtaa tgagcattcc ccattgaggc ttaatgccaa acatggtatc
    12601 taaggtttta ccgcctccca ggaattgagc gcctacataa aggaagaaaa agaaaacaat
    12661 ggttgaacta ctcaccagcc gtatccattt ggcaacctca ccatgacgtg cagcaagata
    12721 atcggaaaaa gtggtagccg aatacttttc ggcttcatcg cgcaggcgcc aagccaaaag
    12781 ccaccaagcg gtaattattc ccacaacaca accaattgca gtccacaact cagctattcc
    12841 cattgcgtag gcagcacctg gtagtcccaa cagcgcccac gacgattcgc cagttgccct
    12901 ttcggaaagg gcagcaaccc aacccggtag gtttcggccg gcaatggagt aatcggcatt
    12961 attcttaacc tttcgtcctt gataaatccc ccacccaatg ataaaaagta agtaaccaat
    13021 ggttacaagt gcaagttgaa tgctggctga catagtaaaa aggttttaac attatcaccc
    13081 taaatttatt aaatagccga tataaaaacc aatgcttgct gttgattttc acctataaaa
    13141 ataactaact tgtgaactgg caaaacaagt gaggtatgga agcattatat tacatgcacc
    13201 tagagaataa ggcggtacaa tgcaatttat gccctcatga gtgtaaactc acccccggta
    13261 agagaggtat ctgcaaaacc cgttataacg ataatggcga aatgagactc ctgatctcag
    13321 gagtaatagc atcgtcagga ttagatccca ttgagaagaa gccccttttc catttctacc
    13381 ccggtagcaa aatttactca attggtggct atggctgcaa cctaaggtgt tcattctgcc
    13441 agaactatga aatatcgcaa tatgttcctg actcaaatag ccgattaaga gaaaatttgc
    13501 cggaaaatat tgttgataag gctctgtttt taccacagaa cattggcatc gcctacacct
    13561 acaatgaacc aaccgtgttc tttgaactta tgcttgcaac ggcacgattg gctaaaggca
    13621 aagatttaaa aaatgtaatg atatcgaacg gatttattaa ccataaaccc ctacttgaac
    13681 ttcttgatgt tattgatgca tttaatatcg atttaaaagc ttttaccaat gagttttata
    13741 aaactcaagc aggcggaaag ttagccccag tgctaaacaa tttaaagacc ataaaaaaga
    13801 atggcaacca tttagagatt gccttcctgg ttattccgga acttaacgac tcaaccaatg
    13861 aagcagtaga gatgtttagg tggattaatg aaaatttggg taacgacaca cccttacaca
    13921 tcaacaggta ttatcccgct tacaagttaa gcatccctcc aacccctcct gaaaaattgg
    13981 tagaacttcg agatgttgcc atggaatacc taaaatttgt atatattggc aacgtccaca
    14041 actccgatat cggcacaaaa acaacatgcc cctcgtgtaa caccactgtt attgagaggc
    14101 atggatatag tactacattg aaaatgattg acactagagg atactgcaag atttgtggat
    14161 acggccctat agcagtaatg ggctaaccac gccaaatcag ttcacttaac tttcgcttgg
    14221 gaacatggtg tacctgttct gaatcgcgcc agtaaattac acttcccgaa tcatcaatat
    14281 catcaataac aactttctca actggtttgc caagggcaat caccagtgcg acctttagat
    14341 attcaggtag attgagtaat ttgtgtaaat tttctttttt aaccgatgca ataatgcacc
    14401 ccccaagccc cttttcaacc gcacccaaaa gaatggtttg cgccactata ccctgatccc
    14461 actgcgatgc tgttgacaac ctctgatcct caagaactac aatataagcc gatggacgtt
    14521 ccccctcaac agggccatcc caatccttca agtaacctgc ccatgccaga ttaggaaaca
    14581 aattgttgca ccattcagga gtattgataa tcataaactt aagtggctgt aggttacggc
    14641 ccgaaggaca aaacctacac agctcaacca gctgttcaag cagccctaaa tcgatattat
    14701 gggattggtc aaaccttcga tagctgcgat tttgaactac aagatccctt agcatgttta
    14761 cctaaccttt tcaaagcgtt tttcaaccac actccagtca acaattttcc agaaagcttg
    14821 aatgtagtcg ggtcgacgat tctggtagtc gaggtagtag gcatgctccc acacatcgca
    14881 ggttagtaag ggctttagcc cttttcgtaa cggattaccg gcatttgatt cctgagtaat
    14941 gttaagtttc ccatcggtat caaccacaag ccaagcccaa cctgaaccaa aaagggtggc
    15001 agcggcattg gagaacttgc ttaaaaattc ttcaaatgaa ccaaatgaag cctcaatagc
    15061 ttttagtaga tcaccctcgg gcttatgctt tggaaccgaa ctaaaagcct caaagtagaa
    15121 cgtgtgattc caaacctgtg cgccattgtt aaatacacca ccctcagcct ccataactat
    15181 ttgctcaagg ctagcattct cgaactttgt tccgggtatc aggttattca ggttgttaac
    15241 gtaagcctga tggtgcttcc cgtaatggaa ctccattgtt ttctccgata ttacctcagc
    15301 taaagcattc agtgagtact gcagctttgg aagttcatgt ttcatagcta tagtttttaa
    15361 attggtttaa ctgccaaaat gagtatgtcg tcggtttgcg cctcctcctc acccttccat
    15421 ttttctagtg ttctatacag tgtcatcttt tgctcgtaga gaggaagatg cttaatgctt
    15481 aaaataagat tacggaatga atggtaacca aattttttac tgggtttacc accaaactga
    15541 tcggtaaatc cgtcggagaa taggtaaacc gtatcgttag gctctaactt tattgaaagt
    15601 tcgttgaatc gctccatgcg ttcatagtaa gcaacgggca ttctatcggg acgaaactca
    15661 ataagccctt cgcctacctg aacaatccga ttatcatttg ggatattaga atttgtgctg
    15721 ataataattg gcatgttggc tcctgaatag ttaagggtat gtgtactcct atcgaaagta
    15781 cagaaagcta catccatgcc atcatggctg tcgtcctcgt gtccaccttg ccgtaaggca
    15841 gaaattatgt tatcgcggag tttaccgagt agcaaacccg gcgatgatgt gggagtgttt
    15901 gcaacaaggt cgttgagcat tgaaatccca agcatactca tgaagccacc gggaacgcca
    15961 tggccagtac agtcagcaac cgcaaatcca attttaccgg agcagttccc gacccagtaa
    16021 aaatcgccac caacggtctc cttaggaaga taaagcacaa aaaagtccct aaagtactgg
    16081 cttagcagtg catcggttgg gaaaactgcc tgctgtattt tgcgggcata ctcaatggat
    16141 tctcccaaat ccgttagtat gttaatgatt cgatcgcgtt ggtaaactac aaggtttttc
    16201 tgcctttcaa gttcatcgcg ttgttcctga agctcacgct gtttgatatc tactgaaatt
    16261 cgctggctct caacctcaac caaggcctgc ttgcgtttat acatagtata tagcagtccc
    16321 aggaacaacc cgaggagtaa aacaaaagca atggcaacca gtatcaacag gcgggttcta
    16381 ctctgctttt ggctatcaac caagctaagc tgctcattga acctctcaaa atgctgacga
    16441 acaatatttt cgtactcaac ctggagctga gctgcgagaa tctgcttcat cttttcagtg
    16501 cctgcaattg agtccttaac cgcaatgtac ttttcgtagt tctgtaaagc cccctgacaa
    16561 ttatttttgg ctaggttgaa ctggtaaagc gcattatacg attgggccac catatccatc
    16621 atcccgtgtt ttttagcctg ttcaaggacc tcatcgacta gtgcgaaagc cttagggcta
    16681 ttgcccattt ggaaaagtag aagagcgtaa tcgtactttg actcaaccac accacggggt
    16741 tcttcgagtt tagtgtatcc ttcaattgct tttgtaagat atagctgagc cttatcgtac
    16801 attttgattt tcatgtaagc ttcgccaaga aaacggttga tattggcaac cccaccccta
    16861 tcgagcagaa tctgcttaag cgcaagagca tcttcaaagt aaagcaaggc gctatcaact
    16921 tgccctaaaa gcaggtaaac cgatccaata ttcatcaatg aattggaaat tcctcgctta
    16981 tcgtttatct caatttttat tttccacgac ttcctgagat aatcaagaga cttttcatac
    17041 tgagccacat cgcggaatat tagacctatg ttgtttagta ggaatgcttc gccctgtctg
    17101 tccttggttt caacccttag gggtaatgct ttgaaaaaga aatcgagggc agtagaatac
    17161 gcaccctgct cccagaaaag gattcccatg ctattatata cggaggcaat accagcttta
    17221 tcgttcaatt tctcgcgtat tgccaaagag ttagagtaat gttcaagggc ttttgtatag
    17281 ttaccaattt gagcattaac catacccaaa ccatagttag cttcagccaa accggtagaa
    17341 tcggcaatag ccgttgcctt attaagtgca agattgtaat aatgcaatga gctatcgttc
    17401 aaatcggttc ttctataaaa tattcctaag cgattgtatg tttttattaa agctatttga
    17461 ttgttgatga ggctaaaatc agcttttgct ctatttagcc agtaaaaagc ggaatcaatt
    17521 ggtgttattt caaagtaaat gattcccaag tagtactcac accaagcacg cgcctcatta
    17581 tattctgaag cgcttgtatt ggctaatact ttttgagcaa tttttttggc agcgctgaat
    17641 tccctgacat caattaacga ccgtacagta gcaagctgag aattaacctg cgctgtatcg
    17701 accatcattt tttgcccatg ggctaaaaca gggaaaataa ccgttataaa acaagacaga
    17761 ataagatttt gaatatgaat tctattgaaa aacatagcat aaatattaac ccaataaaaa
    17821 tacaaaaaaa aagcgaccgg taaaaccggc cgccaaacca aaacataaac caaaccaaac
    17881 ttaaaccaat aaaaaactat ttacctgtag caacaagata tttttcttcc accgccttcc
    17941 agtcaatgac attccaaaag ttctgtatat actcagggcg gcggttttga tactttaagt
    18001 agtatgcatg ctcccaaaca tcgagtccga gaatgggagt gcctttaacc tctgaaacat
    18061 ccattaaggg gttatcctga ttgggggatg aaaagacctg aaggtcctta cctgtccaaa
    18121 ccagccaagc ccaacccgaa ccaaaacggg tagctgcagc gtttgaaaat ttttccttga
    18181 actcatcaaa agagccaaaa aacttttcaa tcccctttag tagctcgcca ttaggctttc
    18241 cacctccatt tggcgacata atctcccaaa aaagattatg attgaagaaa ccccctccgt
    18301 tgtttcggat tgctggtgaa tgcttggaaa ccccccgaag tatctcctca atcggaagcg
    18361 cttcaattga tgtgccagca atggcattgt tcaaattatt agtatatgca ccatggtgtt
    18421 tattgtaatg aatatccatg gtcatttcgt cgatataagg ctcaagcgcc ttgtaatcgt
    18481 atttcaattt aggtaactga aaagccatat gtttactatt taaattgttt ctaaatagta
    18541 aaacgcagct tgataaattt tgttcaaagg attattaaga aatgattact gagagttgac
    18601 agaatttcca tttttcccat taacatatct taccttttcc tttttggttc ggtaaacctc
    18661 aattcctaaa aagtagtagc gaattaccca aaactcaaca taccacttat gatcgagtga
    18721 aagtattgta tcttggaggt gttttgattt tccaataaag ggtcgtttca ttaaatctaa
    18781 agtttcagct tgtcgtatgt aagtacccac aataaattta atttaaaatt aacatctttt
    18841 aagttaatct ccaaacagca attggtttct aacagtaaaa acaaacgatt atctgatttg
    18901 tttacaaggg tttatgttat tcgtaaacaa aaacgctcct ttcgtcaaaa ttatgtgtgg
    18961 gtttgctgct acttatgagc tgtattatgg ccttacgtcc tacttggccg agggttatgg
    19021 agaagtcatt aacatataaa ctgatatggt tgcgcattac ctcagtgctt agctcctggg
    19081 caaactgtga aacaaatccc atagtatttt ccggatattg aaatgcatat tcaacactct
    19141 cccttaacaa ccggttaata tcgtgtttta catcatcagg taaagaccga cgaattgcaa
    19201 tacccccaag aggcgtaggt aatccagaaa ttttctccca ctcatcgcct aaatccatta
    19261 gcttggttaa tcccttaaat tgataggtaa atcgtgattc atgaattatg acacccgcat
    19321 caacctcatt gtttagaatt gcgtcttcaa tctgtgagaa taggtaaaac ctcttatcgt
    19381 gcacatcagg gaatactaag ttaaacaaaa ggtgagcaga ggtatgttca ccgggcaggg
    19441 caatacgttt gtgcttaacc tgttcaaatg gtatggtttc cttgctaata agcaatgggc
    19501 caaccccttt gcccagtgca cttccggaat cgagcaattg gtaatgctgg gctaataaag
    19561 ggtatgcacc atagctaacc ttggtaatat cgggtttagc ctcgagggct aaaagattca
    19621 aatcctcaat gtcggccatg taaacgtcaa aggtgtattt atcagaaagc aacccatgca
    19681 ccagtgcata aaacataaaa gtgtcgtttg gacaagttga aaagttcagc gataggttca
    19741 ttctacagtg tatttaagta actcacaagt aagactagct cattccaaag gttttgaaca
    19801 gcaatgtcga ttttccagtt ttggcgattc cggggttcaa cataattgga tacagccctt
    19861 aggcaaaaaa atggtttacc aagttttaag caagcataga aaaccgcagc cgactccatg
    19921 gtttcaatct gagcatccca tgaacgttta atcatagcga ttcgctcatt agaacccgaa
    19981 acggttgaaa cagttattcc cctaacccca aataacttcg ggtttggctt aatagtgtat
    20041 gggttaacca actctcttaa gggtaaagcc ttttgcaacg ggggtaaaat attatcgata
    20101 tgtcgaaaag tgccattatc atctacacca taatcggcaa aagaatccga atcaataaga
    20161 actacattgc ctaatggtaa ttgctcagta aaacttccag caattccccc gttaataatg
    20221 atatcataag catcggctgc tctggaaagg gcaaatactg ttggaactgc tcctggtccg
    20281 ctaatcagaa tatcggcatg aataccatac tgttgaagcg agtaaaaatc attacccggt
    20341 tgcctgtact ggggcaaaat ttctacttgg ctggcaacaa ctatcaaaat ttttttcaaa
    20401 aactgattac tttttaaaat aaatagttaa acaataaagt aaaagtgttg ttaaaaaata
    20461 atggttaaaa ataaataatg ataggtattt taacaaaaac aaaattgttt gtaactttaa
    20521 aagaaaatgc ccattgcgta cctgacacga agagaacgat ttagcgctgc tcacaggtta
    20581 tacaagcaag aattgaccga tgaagaaaat tttaggctat ttgggcagtg ctcacacccc
    20641 aactggcacg gacataacta tgagcttttt gttacgatta aaggtgaagt gaaccaagtg
    20701 ctgggttatg tggtaaacct caaagtggta agtcaaatca taaaagagcg gataattagc
    20761 aagctggatc acaaaaatgt aaatattgat gtagacttta tgaaaggagt gcttgccact
    20821 accgaaaacc tagccatagc aatatggaaa gaacttgaaa aaccattgag tgacgtagga
    20881 gcacaacttc attgcataaa aataaaagaa accgagaata actttgtaga atattacgga
    20941 gattaaattt atggagatag taaatcaaaa cgcatcgttc aacgaagatg aaggctactc
    21001 aaagattgag aggtggaacc ctgagacaac cgaaaagcta gcctaccact acaaggaaat
    21061 acttaagcta attggggagg atgttggtcg tgagggatta ttgcaaactc ctgtaagagt
    21121 ggcaaaagcc attcagttcc tgacccatgg ctataatatt gatccccatg tgcttgtaag
    21181 aagtgcaaag ttccacgagg agtacaaaca gatggtgctg gttaaggata ttgaactata
    21241 ctccatgtgc gaacaccaca tgataccatt ctacggaaaa gctcacgtgg cctacattcc
    21301 aaatggttac attacgggtc ttagcaaggt agcaagggtt gttgatgcat acgcgcgcag
    21361 gttacaggta caggaaaggt taacagttca aatacgcgat tgcttgcagg aaaccctcaa
    21421 tccgcttgga gttgccgtag ttatagaagc agcccatatg tgtatgcaga tgcgtggaat
    21481 tcaaaagcaa aactctgtta ccacaacatc agcatttacg ggtgccttcc ttacccacaa
    21541 cgaaacacgc gaggagttca ttcatcttat tggagttaag ctccattagc gtgatgaacc
    21601 agaataaaac caaagggtgc ctcaaaattt gaagcaccct ttactttatg tgaatggtta
    21661 atgactagtt gcgttgctct accttaagtc cttttggacc cttgttgccg gtttctctat
    21721 caccaccctc gtcgttattc tttttacgca acttgaagta taggtcaacc atatcaaatt
    21781 tataggttag agcaaactga aatgaacgaa agcgcgattt agtattctca tccatttgtc
    21841 ggtaaatatc aaccataccc aagtcgtaac gtaaatctaa cccccaaatg ccatccttgg
    21901 ttgggtattc ggctccgcaa cccactgaaa atccccagtc gaaacgggtc caatgataat
    21961 cgtcgtaaac tgcaccatcc tcgtaaataa ctttaccgct accaattctt gtagtagagt
    22021 aaacaccacc atttgcatat actctcttta accccagcaa gctaaaagtg tacttggcaa
    22081 gtaagggaat ctgaattgca ggcatacgct ttgtttcctc aactgtccca tagattcctt
    22141 ttgttccttt ggaaataaag ttcaactccg tttgaaagga aaacctattc gtaaacccaa
    22201 aatcaccaac aataccgaac tgataaccaa ttctgctttt cacttcatgg gtgggatatg
    22261 accactggct aaattttgaa aagttcatac ccacctttgg gccgacgtag ataaacccaa
    22321 catcatgccc aaaaacctta aatagttgag cattagcagc attaaaagta attgctacaa
    22381 gtgaaagaat aactaaaatt tttttcatgg tgcaaggggt taaatttcta caaggttaat
    22441 gtaaaaaaaa ttaccatcaa acttttaaca aatttttttc atcaaactct tgactcaaat
    22501 cataattacc aataacttgc gggctataaa tcagaaatat gggcatttta gaaattgaaa
    22561 aggatataca tgcccgcata gtgcgcagtg tgaatgccca gctaggcaac tacataacct
    22621 ttaataagaa ttgcgtcatt gaaccaattg agcaaacatt cttccccaac tcaacactcc
    22681 tatccataat tgatttcgat tgtattccag catcaaaact aatggtaatt gacaatggag
    22741 ttcaggttta cccattaagc ggattagggc aatggaactc aatactgatg aatgaaaggt
    22801 taaacctaaa cattaagaat gtactcgatt accttaagct atactactca actcacgttt
    22861 tcccatacca gtatcaaaaa atagttgaaa ctattcacga tcttgatttc atagaatttc
    22921 ctgaaataaa acttttcgag gtgataaacc aacgaattca agccccagtg gtaaccctct
    22981 caaacaagca attcacaata tactgtaata ttacttccaa tcgaacatta tacaatgcag
    23041 catttgatgt ttatccggat ggtaaagttt tactgctcga taaaaagatt gttatgcaag
    23101 accttcccat aggcgaaatg gtaattgatc tttaggccta cgagtgagta tctcctaaag
    23161 ccattgtacc attacaattt caaatttctt ggtcacagaa attaaaaaat gccttacatt
    23221 tgcccttt
//
LOCUS       DAOOWJ010000034        23001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2258_length_23001_cov_7.057614, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000034 DAOOWJ010000000
VERSION     DAOOWJ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 23001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23001
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2258_length_23001_cov_7.057614"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLW22_05045"
     CDS             complement(<1..429)
                     /locus_tag="PLW22_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOU74106.1"
                     /translation="MIVLVLNCGSSSIKYQLLNMANDGQLLAKGIVERIGFTDAILTH
                     KPEGKDRYEVVTSIPDHTTGINLILEALVNEKHGVIKSLNEITAVGHRVAHGGEFFTK
                     SSLIDDFTKSEIEKCIELAPLHNPANLKGILSMEKLLPGVP"
     gene            765..3686
                     /locus_tag="PLW22_05050"
     CDS             765..3686
                     /locus_tag="PLW22_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOU74107.1"
                     /translation="MKDLEIKINELSDLLSCEVKWDESTLIQYSTDASAYKEKPYAVV
                     WPQDVNDIQQVIRFAKKHKIPVIPRAAGTSLAGQVVGKGIIIDISKHFRRFINLDIEK
                     RRVTVEPGVILDELNLFLKPHGLFFGPETSTSNRCTLSGMVGNNSCGSHSLVYGSTRD
                     HLYSVKMVLDDGNEYLFGEVNKTEYDEKLRLSNREGDIYRYIHNLIGNEQNQKLITSN
                     FPDPSVKRRNTGYALDTFIGTVAAGGELPLNLAKLIAGSEGTFGIITEITLNLVPLPP
                     KEKAVVAVHLKNRTDAFKANLIALKHKPWAVELMDNIILECTKGNIEQQKNRFFVQGD
                     PGAILIVEFFADSKGEIESAAQKMESDMREAGYGYHFPIIWGAETSKVWNLRKAGLGV
                     LSNVVGDAKPVSLIEDTAVPVERLPEYMDDFEKIMQRYGLECVYHAHIGTGELHLRPV
                     LNLKDPKDVELFHTIGYEVAMLVKKYKGSLSGEHGDGRLRGEFIPIFYGEEVYQLMRE
                     LKFAFDPDNIFNPGKIIDTPPMNSSLRYIPGQKERVIDTIFDFSSSLGIIRATEKCNG
                     SGDCRKTHLSGGTLCPSYMATLNEANTTRARANLLREFLSYSPKRNPFNQRELYEILD
                     LCLSCKGCKNECPSSVDMAKLKAEFLQHWYDANGIPIRSRMVAYIAHINRMGSYFPRL
                     TNWFLSNKVTSKILMQAIGFEPKRKMPLLGESTLKKWFKRNRCHSAKHNGKVYLLPDE
                     FTNYNDVHIGIKAVELLNRLGYEVELVDIFESGRTYISKGLVRTAKRIANSNIEKLSG
                     IINPDTPLIGIEPSAILSFRDEYPDLVSQENKQKANSLAENSLLFEEFFIREAAKGRI
                     NPEMFTTRAQHIKLHGHCQQKAIASTTPTKAMLSFPVNYTVEEIPSGCCGMAGAFGYE
                     KEHYDLSMKVGELVLFPEIRKTPLDTIISAPGTSCRHQIKDGTGRKALHPIEVMWEAL
                     V"
     gene            4149..5243
                     /locus_tag="PLW22_05055"
     CDS             4149..5243
                     /locus_tag="PLW22_05055"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU74108.1"
                     /translation="MKRVLVATGGGDCPGLNAVIRAIVKRASQEPDWEVIGSIQSFDG
                     ILREPTEIKVLDERAVAGIHVQGGTIIGTTNKGGPFAWPVKNKDGSWGAVDRSDEMIR
                     KLQYLGIDAVISIGGDGSQRISQQLFEKGLNIIGVPKTIDNDLSATDFTFGFQTAVQI
                     ATEAVDKLVTTAASHNRVLILEVMGRYAGWIALHAAIAGGAEVCLIPEIPYDIHKVAE
                     RLQSRYNKGKGHAIVVVAEGALPKGGTLVSAESDEVGYHNLRLGGVAHKITRDLKSIG
                     FEADIRETVLGHLQRGGIPVAYDRVLATQFGVKAFEMVLEGKFGEMVAYRHPDIISVP
                     LIDAINRPNFVDPDCDLVRTARGVGISFGE"
     gene            complement(5321..5734)
                     /locus_tag="PLW22_05060"
     CDS             complement(5321..5734)
                     /locus_tag="PLW22_05060"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU74109.1"
                     /translation="MEPLRIKARKDSPDITFDPQSGNFTIIGVSHPENVTSFYEPVVK
                     WLTQFKIEIQKNGAKSVKPINFRLFFKYINSASYKYMITLFQHMQEIFEMGVPIQIVW
                     NYEPGDEDMAESGWELIEYSGIKAPSVCEVCNDPI"
     gene            complement(5747..6130)
                     /locus_tag="PLW22_05065"
     CDS             complement(5747..6130)
                     /locus_tag="PLW22_05065"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU74110.1"
                     /translation="MERLHLEPTLTTPKVIFDRDSGEFTIQGKSLPEDVKSFYTPLIA
                     WLDDYMKNPNKTSLFEFDFEYFNTASSKMILIIMNRIKELFKKGYEVQIVWKYPQQDA
                     ELEEAGEELAELISVPFKLVPKPEM"
     gene            complement(6143..6532)
                     /locus_tag="PLW22_05070"
     CDS             complement(6143..6532)
                     /locus_tag="PLW22_05070"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU74111.1"
                     /translation="MDALIIKGTNETPNITFDKDANKFEITGKSLPEDVKEFYNPVLR
                     WLKAYAEDPNPKTVFKVKMEYFNTASSKMLLEVLELLDGMYSAGKDVEVEWHYMEDDE
                     DMHDAGNDYADMLELPFKMISYQHISK"
     gene            complement(6673..7974)
                     /locus_tag="PLW22_05075"
     CDS             complement(6673..7974)
                     /locus_tag="PLW22_05075"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HOU74112.1"
                     /translation="MSDRGFIFNYSRCVGCHACIVACYNQNHTEPPMAWRMVVNGNPL
                     KIPLKGFINLSLACNHCIDAPCMTNCPAIAYSRDDETGAIIHSPLKCIGCKYCTWACP
                     YEAPKYNPGKGVVEKCNFCNDLLKVGGIPACAAACPTGALTFGEIKVEANLSKPGFPE
                     VATSPRINVANESINDSLPEMAIEATGLQQSDFAETYSPRIHPTKEIPLFVFTSLSAV
                     LVGWFITFYRLEKISYFSKISFILLLAFAGFASLFHLGKPLRAIRALLHVKSSWLSRE
                     IALFGLFAFSGLLYVLTEKPPLFWFSVVVGTVFLISLEMVYHVVRKNYSTPIHSANTL
                     LTASTLFSLITLSKAFVLLATIKLLLYLVRHAYIQKFNPKKVIFSFIRFLGILIPLVG
                     LLFNLIPANIAPFLTLFLIGEFIDRYEYYASIDTHNPFQSI"
     gene            complement(7971..10019)
                     /locus_tag="PLW22_05080"
     CDS             complement(7971..10019)
                     /locus_tag="PLW22_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU74113.1"
                     /translation="MKEFITACPRNCYSTCSLVVQVDKGKVVGINHLPLNKATPFGGC
                     LKGLSYVERANSPDRIIYPLKKVNGSFKRISWNEALETIAEKLIHFRDVYGQQSVLFY
                     AASGMAGLLNDFSGKFWRYVYGGATTVYGNLCWPAGLEATRLTLGENKHNVPWDLENA
                     KLIVLWGKNPAESNVHEMIPLNNALDKGAELVVIDPRRTQSTQRATLLLQPYPSTDAP
                     IALAVANIIIQNGWIDSDFIKLHVHGFDQFAEHVKPFTAQWASNISGVPVSLIEKLAW
                     YIGNVKPMTLIPGYGMQRYTNGGQTVRAILALSVITGNIGKSGACWHYANLQSYVFDD
                     VLEPLCYYPPDKPDGVIRRSISTARLGEDMLATKNPELKMIWVERGNPLTQNPNTGKV
                     LEAFRKLDFRVVVEQFMTDTALEADIILPAKNMFEQSDIVGSYWNPYIQLKQKVVEPA
                     GEVKPETEIYWWLAQKLGFNEAELSNILLPPGDEHVDRYLDSKLREFNLSLEELRKGP
                     ILAPGLQEIAFSDYRFPTPSGKIELYSEQVQTLWGENPLPTYKPAVELFQNQTPYPLF
                     LMSPNTKNRIHSQFNNLKVISMISGAPVLQINPADAIQRGIGDGDLVEIFNSRGSFRV
                     KAQLTYEVRVGCVVIPNGWWRTQGAPVNVLSEGRETDMGHGSAFHNNRVEVKLVKRKP
                     "
     gene            complement(10026..11726)
                     /locus_tag="PLW22_05085"
     CDS             complement(10026..11726)
                     /locus_tag="PLW22_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74114.1"
                     /translation="MKSIISKAFFTVSVIFLLCLSAIAQNGLQVSARLIVKKGDLQGS
                     KVTVTQNGNVVQTISVSNKGVFDFILEYNFEYIIVFEKPDYITKKVLIDTRVPETFRK
                     DIQQMTGFDVVLEKQSEDVIKEYANPVAKIMYARDIRDFTYDTDYSLKVLKEIENENK
                     KLAEEKKKKEEEEKARKAEEEKQRILAEAQKRKEEEEAKRKAAEEARKKEEERLRKEA
                     EERRIAEEKKKLEEQKRLEELKKKAEEEARLKAEEEARKKEELRKQEEERLRKEQERI
                     RQEEEAKRKAAEEARLKEEERLRKEAEERRLAEEKAKLEEQKRLEEQRKKAEEEARLK
                     AEEEARKKEELRKQEEERLKQEQERVRQEEEAKRKAAEEARLKEEERIRKEAEAQRLA
                     EEAKRKAEEEAKLQEAERLKKEAEERAIAEAKLREERLKAEQEKARQEAERLKREEEE
                     RRRAEEQKKLLLEEQRKRDAELRLKLVAEKMSAQIGKEDADQVVTLLAAEDIAKRYTR
                     ERTDEVIRYPRIILTRTVFNRNNTITFYTRVDHEYGAVYYLKDGFFISKEQYLAETQK
                     "
     gene            complement(11825..12814)
                     /locus_tag="PLW22_05090"
     CDS             complement(11825..12814)
                     /locus_tag="PLW22_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74115.1"
                     /translation="MVLLLFSNLADGQPVFADTSLLDVKLEFGIQQVFADIGEDPSYH
                     KGYLSYENENGATISIPVEIRTRGIFRRKASNCSQPPLLIKFKPKETSNTIFEGIEKL
                     KLVVPCQKSSKYEDLVLKEYLVYKLYQIISPYSYRVRLLRLNMVDRYYGNQSICYAFV
                     IEPVEILSRRLGGVVREAKNTHPNACNSYYYNRMAIFQYMIGHTDWSIKALHNITLIE
                     PEPFAPSIPVPFDFDFSGFVDAPYALPAEHLPIKSVKERHFNGYCKPEQQFADAFSYF
                     LNLKDTINHTITTFKYLPQAQRNELIRYTNEFFDIIASDTKRKTRIITKCRTD"
     gene            complement(12894..13997)
                     /locus_tag="PLW22_05095"
     CDS             complement(12894..13997)
                     /locus_tag="PLW22_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU74116.1"
                     /translation="MRRILILLQKEFIQIFRNKTILPIIFVLPIVQLLILANAATQEM
                     KNIRLTIVDFDNSSESRRLINAFDQSKFYTLIPVSGNVDRAVELIDLGKTDAALVIPK
                     RFGESLVEGAPVNIQLMLNAINITAAALTQSYTTGIIQNFASSISIQNINHIKPKQSV
                     RYWYNPELNYKLYMVPGILVILVTIVGMFLTAINIVREKELGTIEQVNVTPIKPYQFV
                     IGKLLPFWIIALFELSFGLLIARLVFNLTIAGSIPLLFAFAAIYLLVALGLGLILSTS
                     ASNQQQVMFMAFFFMIVFLMLSGLFTPTESMPKWANWFNTINPLYYFMKVNRMLILKG
                     SDFTNLKDSFLSISLMAVAVLSIAILRYRKKSA"
     gene            complement(14009..15121)
                     /locus_tag="PLW22_05100"
     CDS             complement(14009..15121)
                     /locus_tag="PLW22_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU74117.1"
                     /translation="MAKGRFSNFITKEFKHIFRDTRTLLIVLVMPVLQLLIFGFVISS
                     EIKNANYAVLDYTNDYNSKKLVQRISSSGYFNFKGYLKSENEINEVFKNGDAKLVLII
                     GNNFTQKLQNENFANYQVVTDASDPNTASILASYAQGIVNSFTAEYFGSSQQKERVNV
                     QSRMLYNEEMNSAFMFVPGTMVLILMLVTAMMTSISITREKEMGTMEILLVSPLKPWQ
                     IVVGKVIPYIGIAFTNVLVIILMGYTVFHLPLRGNFLLLMGVSSLFIVLALSLGILIS
                     TLSNSQAVAMFMSMFALMLPTILLSGFIFPIENMPKILQWLSLIMPPRWFLSALKTIM
                     IKGQGLQYVISEVLVMLTMTAVFIALSIKKFKVRLE"
     gene            complement(15124..15864)
                     /locus_tag="PLW22_05105"
     CDS             complement(15124..15864)
                     /locus_tag="PLW22_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU74118.1"
                     /translation="MTNAIEVENLVKRFGSFIAVNHLTFSVGKGEIFGLLGANGAGKT
                     TTIRMLCGLLSPTSGKISVAGYDAYTQPEQIRASIGYMSQKFSLYSDLTVWENIRFFG
                     GIYGLWGKELRIRALELVEEFKMHDFKDTKVESLPVGWRQRISLAVSLVHKPPIIFLD
                     EPTSGVDPVGRRMFWETIHQISSMGTTVLVTTHYMDEAEYCHTVLVMVDGRIEAQGAP
                     AQLKSTHGVESMNDLFLLLSRNGKLNIE"
     gene            complement(15861..16781)
                     /locus_tag="PLW22_05110"
     CDS             complement(15861..16781)
                     /locus_tag="PLW22_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU74119.1"
                     /translation="MPAIQVTNISKSYGKVKALTDVSLSVEEGEMFGLIGPDGAGKST
                     LIRIITTLLVPDSGNVTVFGLDTVSQYKLIRGEIGYMPAVFSLYSDLTVEENLNFFAG
                     VFGTTVKENYELVKGVYHMLEPFRNRPAGKLSGGMKQKLALSCALIHRPRVLILDEPT
                     TGVDAVSRKELWDLLHSIKKQGITVFVSTAYMDEAKQCDRVALIQKGRILGTNTPEGF
                     AEMFPYKLFSIQANNAWSSLDAIRHLSTCETAYMFGDSIHFATNYNEESPESLHDRLM
                     SVGVEANVAQIQASIEDYFIYQLSEKNLVK"
     gene            complement(16912..17805)
                     /locus_tag="PLW22_05115"
     CDS             complement(16912..17805)
                     /locus_tag="PLW22_05115"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU74120.1"
                     /translation="MNWKNLINVILPTAIVISCSRDNGKADAYGNIESNDVTISAEVG
                     GKLLELKIDEGYKVEKNQLIGIVDTLPQVLKLKQLNAQLKAAKAKLQGIDAQIAVQDE
                     QLNVLKKELDRVKKLLADSAATLRQLDDIEGKFKIAEKQKATFDAQRSGVLAEIDAVD
                     AQIAQALDLLWRCHMVSPVSGTVISRYVSQGELVITGKPVCKIGTLDTVYARVYIDET
                     QLPDFSIGRKVTILTDTSDGKLKEDKGEIAWVSSEAEFTPKIIQTRNERVNLVYAVKV
                     RVPNPNGYFKIGMPVEVRIED"
     gene            complement(17818..19098)
                     /locus_tag="PLW22_05120"
     CDS             complement(17818..19098)
                     /locus_tag="PLW22_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU74121.1"
                     /translation="MRKARIISLTITLLASTYMLKSQNTVTLPGLLKSMEQWNKLYRV
                     SAATDSVYWLRKSNIKVNYLPKVELNAEATWQSDVTKVNIPMPGISIPTPDNDNYKLT
                     IDVSQLIWDGGATKSMHQLEDESRKLEQNKVESELYTQKERVASLYFGILSTDMAIRQ
                     LKAMAVELDRRIEELESGVNAGVVLESSANALKAERLRLEQSIDANLSQRYSLTESIY
                     SLTGVKIDESAQGIIPSLAIPNQDVCSRPDYKLFDLQNGYLSTAAGALTSRRMPKLSA
                     FARVGYGKPGLNMLSNEFDSFALVGARLSWSIWDWNSTSRERQTLKIQQNILQYRRNA
                     YDEGCSAQVSSALAQIQSLQRQIQTDEKIVELLEKSVKASASQLKNGTITSSVYLSDF
                     NSLLRAKIDMDLHKIKLSQEVVRLYFTLGMSIND"
     gene            complement(19107..>19481)
                     /locus_tag="PLW22_05125"
     CDS             complement(19107..>19481)
                     /locus_tag="PLW22_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74122.1"
                     /translation="QKIRHFVEEYIELFLRNPFLPAFVLREANRNPEMIAQFITESGI
                     NAEKLKDVMKVLSHELGLSVNEVLHLMVTTVSSCIFPFAGKPIIEQVLFNGNEGDYQK
                     FLSERKRYVADFMIYFLKGRLK"
     assembly_gap    19482..19581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19749..20792
                     /locus_tag="PLW22_05130"
     CDS             19749..20792
                     /locus_tag="PLW22_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOU74123.1"
                     /translation="MDIHTPIVINGINIKNRVALPPIVNFGWSDSDGKVSSKHIAHYE
                     RIARGGTGLIIVEATCVNPTGRIFSYQLGIWDDNHIEGLARIAEAVHKHDAKVLIQLH
                     HAGLTTRKAVAEKAFGPSVDPENDRSVALTKEQVSELVENFVSAAKRAHLAGFDGVEL
                     HGAHGYLLNQFASSAINHRTDEYGGNSENRLRFARQIIEGIKEVTPSTFIIGYRMAGC
                     SPTLNDGIEIAKLLQAYGVHYLHVSHGGEKGINPTVPSNFDYNHVVYMGTEVKKFVDV
                     PVMVVNQIKSPERANKLVTHGLADMVAIGRDMLTDPDWVTKAKTGEAINLCIDCQPRC
                     KRYTKPESCPLAE"
     gene            complement(21155..22570)
                     /locus_tag="PLW22_05135"
     CDS             complement(21155..22570)
                     /locus_tag="PLW22_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOU74124.1"
                     /translation="MNTFLESFDYVIQLYNEYVGGYLILALLVPTGIYYSIRLKFLQV
                     SHFKHAIDLVRGKYSKHDDVGDVSHYKALTTALSATVGTGNIVGVTLAIYFGGPGAVF
                     WLWVTGFFGMMLKMVECTLAQKFRKINADGTVSGGPMYYIEHGLKDKLGVWAKRLAVF
                     FAFAGILCSLGTGNMAQANSISDVLKTSYNIPVLYTGIAISALVFLIVVGGLRRIANV
                     TSKLVPLMAVIYFGSAILVIGVHYADIPSALWLIVKSAFTGQAAVGGFVGSTFFMTMI
                     WGVRRGLFSNEAGQGSAPMAHAAAKTPYPMREGFVASLEPFIDTIVICTLTALVIVLT
                     GVWRLDGAVKGVGMTVAAFQEGLGKIGIGFLARHMVAISLLLFAFSTIISWSYYGTRT
                     AQYIFGDKAIKPYYYVFTLFVFFGSVWGIDLVWHFVDMVITFMSIPNLIALLLLFPVM
                     KAEAEGYLKRLKAGEFNLNRD"
BASE COUNT         6639 a         4960 c         4567 g         6735 t
ORIGIN      
        1 tggaacgccc gggagtagct tttccatgga aagaatccct ttaaggttgg caggattgtg
       61 aaggggggca agctctatac acttttcaat ttcactcttg gtgaaatcgt caattaagct
      121 actcttggtg aaaaattcac caccatgagc cacacggtgt ccaacagcag taatctcatt
      181 taaactctta attacgccat gtttttcgtt aacaagcgcc tcaagaataa gattaatacc
      241 ggttgtgtga tcggggatgc tggtaaccac ctcgtaccta tccttaccct caggcttgtg
      301 agtaagaata gcatcggtaa aaccaatacg ttcaacaata cccttagcta gtaactgacc
      361 atcattggcc atgtttagca gctgatactt aatggatgaa cttccgcagt tcagaacgag
      421 aacaatcatt gttttattat tttgatagtc aaacaattag ttaattcaat ttacatacgc
      481 aaaattagga ataaatacct tcaccttcaa agcatttaag gctttttaaa gcattaactg
      541 gttgatttat agtaaaacag gcgtttaaaa cacctctaac actctgaatt acattgcaat
      601 atgtagcata tgtttttcaa catggtattt ttttttcttg aattgcttac gatgttatac
      661 ccagttttat attttttcaa aatcaaatca ttagaatcaa aattacggga agaaaatcat
      721 agaaaaataa attgctttaa gtttgtatga atttttaaag aggtatgaaa gatttggaaa
      781 tcaaaataaa tgagctgagc gatctgctaa gctgcgaggt aaaatgggac gaatccacgc
      841 ttattcagta ttcaaccgat gcatcggcat acaaggagaa accctatgct gtagtttggc
      901 cacaagatgt aaatgatatc caacaggtta ttcgctttgc caaaaagcat aaaataccag
      961 ttattccacg tgcagcagga acctcgttgg cgggacaggt agttggcaaa ggcattatta
     1021 ttgatatctc aaagcatttc cgcagattca tcaaccttga tattgaaaag agaagggtta
     1081 ccgttgaacc cggtgttata ctcgatgagc ttaacctctt tcttaaacca catggtttat
     1141 tctttggccc tgaaacatca acatcaaatc ggtgtaccct ctcgggtatg gtgggcaata
     1201 acagctgcgg ctcacattct ttggtatacg ggagcacccg cgatcatctt tactcagtaa
     1261 aaatggtact cgacgacgga aatgaatacc tttttggaga ggtaaataaa accgaatacg
     1321 atgaaaaact aaggctttca aatcgcgaag gggatattta ccgttacata cataacctta
     1381 taggtaatga gcaaaatcaa aagttaatta cttcgaattt tcctgatccc agtgtaaaac
     1441 gtcggaatac gggctatgcc ctggacacgt ttattggaac agtggctgct ggtggtgaat
     1501 taccgcttaa ccttgcaaaa ttgattgccg ggagtgaagg aacttttggc attatcaccg
     1561 aaataaccct aaacctggta cctcttcccc ctaaggaaaa agctgtagtc gctgtacatt
     1621 taaaaaatcg caccgatgcc ttcaaggcaa accttatagc cctgaagcac aaaccctggg
     1681 ccgtggaact aatggacaac attatcctgg agtgcactaa gggcaatatt gagcagcaaa
     1741 agaaccgctt ttttgttcag ggcgacccgg gagccatact tattgttgaa ttctttgccg
     1801 atagtaaagg cgaaattgaa agcgctgccc agaaaatgga aagtgatatg cgtgaggcag
     1861 gctacggtta tcatttccca attatttggg gtgctgaaac ctcaaaggtt tggaatctgc
     1921 gtaaggctgg acttggtgta ttatcgaatg ttgtgggcga tgcaaaaccc gtttcgctta
     1981 ttgaagatac tgcggttcct gttgaaaggc tacccgaata catggacgat ttcgaaaaaa
     2041 taatgcaacg atatgggctt gaatgcgttt accatgcaca cataggaacg ggagaactcc
     2101 atttaaggcc cgtgcttaat cttaaggatc ctaaggatgt tgagctattc cataccattg
     2161 gctacgaagt ggccatgctc gttaaaaagt acaagggttc tttaagcggc gaacacggcg
     2221 atggaagatt gcgaggcgaa tttataccca tcttttacgg cgaagaggtt taccagctca
     2281 tgcgagagct gaagttcgct tttgaccctg ataatatttt taatccggga aaaatcatcg
     2341 ataccccacc catgaacagc tctttaaggt atattcccgg tcaaaaggaa agggttattg
     2401 ataccatttt cgatttttcg tcctcgcttg gaataatacg cgctacagaa aaatgtaatg
     2461 gttcaggcga ctgccgaaaa acacatttgt cgggcggaac actttgccca agctacatgg
     2521 caacacttaa cgaagctaac acaacacgcg cacgagctaa cctactcagg gaatttttat
     2581 catacagccc taaaagaaac ccatttaatc aaagggagct atacgaaata ctggatttat
     2641 gcctctcatg caagggatgt aagaacgaat gcccctcgag cgttgatatg gcaaagctta
     2701 aagccgaatt cttacagcac tggtacgatg caaacggcat ccccatacga agtaggatgg
     2761 tagcctacat tgcccatata aaccgaatgg gtagttactt cccccgatta acaaattggt
     2821 ttttaagcaa taaggtaaca tcaaaaattc tgatgcaggc aataggattt gaacctaaac
     2881 gaaaaatgcc cctgctaggt gaatctaccc ttaagaaatg gtttaagcgg aacagatgcc
     2941 attcagctaa acacaacgga aaggtttacc tcctacctga cgagtttaca aactacaacg
     3001 acgtacatat aggcattaag gctgttgaat tgcttaatcg gcttggttat gaggtggaac
     3061 tggttgatat ttttgaaagt ggtagaacct atatttcaaa gggtttggta agaaccgcca
     3121 agcgtattgc caacagcaac attgaaaagc tatcgggtat cataaatcca gatacacccc
     3181 ttattggaat agaaccttcg gcaatactta gttttagaga tgagtatccc gatttggtat
     3241 cccaagaaaa caagcaaaag gcaaatagtt tagcagagaa ctctcttctt tttgaagaat
     3301 tttttattag ggaggctgct aaaggcagaa tcaacccaga gatgtttact acaagagccc
     3361 agcacattaa gttacatgga cactgccagc aaaaagcaat tgcctcaaca acacctacta
     3421 aagcaatgct atcgtttcct gtaaactata cagtggagga aatcccgagt ggttgttgcg
     3481 gcatggcagg ggcatttggt tatgaaaagg aacattacga tttatcgatg aaagtgggtg
     3541 agttagttct gttccccgaa atccgtaaaa caccccttga cacaataatt tctgcacccg
     3601 gaacaagctg taggcaccag attaaagatg gtactggccg aaaagccctt caccccattg
     3661 aggtgatgtg ggaggcgttg gtttaaagga taaaggataa agggtaaagg ataaagggta
     3721 aaggataaag gttaaagatt tgaggttcaa agtttaaggt taaaggggtt aaaacaatcc
     3781 actttgcaat atttcagaac tatctcagca tttctgcttt ctgattttta gtttcatact
     3841 aattattcgg attcttcccc ttcccgtttt cacacgtttg cggtgcataa ttatttgtaa
     3901 catcaagagt tttgatactt tttagcattg tacgtttaaa aaaattcata cctttgcgaa
     3961 cgtggaaaaa atttggcttt ttacaatttg taatatacca aataatgagg taaacatgaa
     4021 caaccagttt taattattga ggtatatcga aaaaatgatt acaatttgta actttttcgg
     4081 cagttttact tcagttttta aaacaaagac tttagcgtaa aaaaccttaa caaacgaaat
     4141 taaaaccaat gaaaagagtt ttagtagcaa caggaggcgg cgattgtcct ggattaaatg
     4201 cggtgatccg ggcaattgtt aaacgtgcct ctcaggaacc cgactgggaa gttattggta
     4261 gtattcagtc gttcgatgga attctacgtg agcctactga aatcaaggtg ctcgacgagc
     4321 gtgctgtggc aggcattcac gtacagggtg gtacaataat tggaacaacc aataagggag
     4381 gcccctttgc ttggcctgtt aaaaataagg atggctcatg gggtgcggtt gaccgttccg
     4441 atgagatgat ccgcaaacta caatatctgg ggattgatgc tgttatcagt attggtggcg
     4501 atggttctca acgcatttca caacagctat tcgaaaaagg gttgaatatt attggtgttc
     4561 ccaaaaccat cgataacgac ctttcagcaa ctgactttac tttcggtttc caaactgcag
     4621 ttcaaattgc aacagaagcc gttgataagc tggttaccac ggccgctagc cataaccgag
     4681 ttctgattct agaggtaatg ggacgttatg ccgggtggat tgcactgcat gctgctattg
     4741 ccggcggtgc tgaggtttgc ttaattcctg aaatcccata cgatatccat aaggttgctg
     4801 aacgactcca aagtcgttac aataaaggga aaggacatgc cattgtagta gtagctgagg
     4861 gtgctctacc caagggtggc acactggtat cggctgaaag cgatgaggtt ggttaccata
     4921 acctccgact tggtggagtt gcgcacaaaa ttacacgaga ccttaagagc attggttttg
     4981 aggccgacat ccgcgaaacc gttcttggac acttgcagcg tggtggcatt cccgtagctt
     5041 acgatagagt tttggctact cagtttggcg taaaggcctt tgaaatggtg cttgagggta
     5101 aatttggcga aatggttgca taccgtcacc ccgatatcat ttccgtgccc cttattgatg
     5161 ccattaaccg tcccaacttt gttgaccctg attgcgacct ggtgcgcaca gctcgaggtg
     5221 ttggtataag ctttggtgag taaaaagtat ttatcaataa caggaaaggg gaagtttaaa
     5281 cttccccttt tttttcaagt ttcagcgtac tttcagactt ctaaatagga tcgttgcaaa
     5341 cctcgcaaac cgagggggct ttaattccag aatactcaat taattcccac cctgactcag
     5401 ccatatcctc gtctccgggt tcatagttcc aaacgatttg gattggtacc cccatctcaa
     5461 atatctcctg catgtgctgg aaaagggtaa tcatatactt atacgaagcg gagttaatgt
     5521 atttgaagaa aagcctaaag tttattggct taaccgattt ggctccattt ttttgaattt
     5581 caattttaaa ctgggtgagc cactttacta caggttcgta aaaactggtt acattttccg
     5641 gatgactaac cccaattata gtgaaattgc cactttgggg atcaaatgta atatctgggg
     5701 aatcctttct ggctttaatg cgtaatggtt ccattatggt atgtttttac atttcaggct
     5761 ttggtacaag tttaaaaggt actgatatca actcggcaag ttcctcgcct gcctcctcaa
     5821 gttcagcatc ttgctgagga tatttccata ctatctgaac ctcatacccc tttttaaaaa
     5881 gctctttgat gcggttcatt ataatcaata tcatttttga ggaagcagta ttaaaatact
     5941 caaaatcgaa ttcaaagagt gaggttttgt tggggttttt catgtaatcg tccaaccaag
     6001 caataagtgg agtgtaaaac gatttaacgt cctccggaag ggatttccct tgaatagtaa
     6061 actcaccgct atcccggtca aaaataactt tgggtgtggt aagtgttggt tcgaggtgaa
     6121 gcctttccat ggacgttaga agttatttgg aaatatgctg gtagctaatc atcttaaaag
     6181 gcaactcaag catatcggca tagtcgttac cagcatcgtg catatcctca tcatcctcca
     6241 tatagtgcca ctcaacttca acgtccttac cagccgaata cattccatca agcaattcca
     6301 aaacctcaag cagcattttt gatgatgcag tattaaaata ctccattttt accttaaaaa
     6361 cggtttttgg gtttggatct tcggcataag cttttaacca cctaagtaca gggttataaa
     6421 actccttaac atcctcaggt agcgatttcc ctgtgatttc gaacttattg gcatccttgt
     6481 cgaatgtaat atttggggtt tcgttagtcc ctttgatgat taatgcatcc atactgctat
     6541 gtttataaaa atcacttttc aacttaataa aacaaatata aacattttac ccaaaatatc
     6601 aatagcaaaa agttattgtt tgaaaataat tttgacaaaa cgccggggaa attccttaaa
     6661 acaacctaag gtttaaatgc tctgaaaagg gttatgggta tcgatgctag cgtaatactc
     6721 gtaccggtca ataaactccc caataagaaa gagggtgaga aatggggcta tgtttgcagg
     6781 aatcaaatta aagagtaatc ctacaagtgg tatgagaata cctaaaaatc ttataaatga
     6841 aaaaattacc tttttagggt tgaatttttg aatgtatgca tggcgtacca ggtaaagcaa
     6901 tagtttgatg gttgcaagaa gaacaaaggc ttttgacaaa gttattaacg aaaatagcgt
     6961 agaggcggtt agcaaagtat ttgcactgtg tataggggtg ctatagtttt tacgaaccac
     7021 gtggtaaacc atttcaaggg agatgagaaa aaccgttcct acaaccaccg aaaaccaaaa
     7081 taatggaggt ttttcagtta aaacgtaaag cagacctgaa aaggcaaaca aaccaaaaag
     7141 agcaatttca cgactcaacc agctcgattt aacatgaagc agggctctta tcgcccttag
     7201 aggcttgcct aaatggaata aacttgcaaa acccgcaaaa gcaagtaata aaatgaagga
     7261 aatcttagaa aagtatgaga ttttctcaag gcgataaaag gtgataaacc atcccactaa
     7321 tacagctgaa agggaagtaa aaacaaacaa agggatttcc tttgtgggat gaatacgggg
     7381 gctatatgtc tcagcaaaat cggattgttg aaggcccgtg gcctcaatag ccatttcggg
     7441 caaactatcg ttgatacttt cattagctac attaatgcga ggcgaagtag cgacttcagg
     7501 gaacccgggt tttgatagat tagcctctac ctttatttca ccaaaggtga gagcgcctgt
     7561 tggacatgct gcagcacaag caggtatgcc tccaaccttt agcagatcgt tacagaaatt
     7621 acatttttcc accacaccct tacccgggtt atatttaggc gcttcataag ggcaggccca
     7681 ggtacagtac ttgcagccaa tgcattttag agggctgtgt attattgctc cagtttcatc
     7741 gtcccttgaa tatgcaattg cagggcaatt agtcatgcaa ggtgcatcaa tacagtggtt
     7801 acatgctaat gaaaggttga tgaaaccctt taatggaatt tttaaggggt tgccattaac
     7861 caccattcgc catgccattg ggggttcggt atggttttgg ttatagcatg ccactatgca
     7921 tgcatgacac cccacgcatc gggaataatt gaatatgaac cccctgtcgc tcatggttta
     7981 cgttttacga gcttaacctc taccctattg ttatgaaatg ctgatccgtg tcccatatcg
     8041 gtttctctac cttccgaaag gacattcacc ggtgcaccct gtgttctcca ccatccgttt
     8101 gggataacca cacagcccac ccttacctca taggttagct gtgccttaac cctgaacgaa
     8161 cccctactgt taaaaatttc aaccagatcg ccatcgccta ttcccctttg aatggcatca
     8221 gccgggttaa tttgtaatac cggtgcgcca ctaatcattg atatcacttt taaattattg
     8281 aattggctat gtattcggtt tttggtgttt ggcgacatta aaaaaagcgg atatggcgtt
     8341 tggttctgga atagctctac ggctggttta taggttggaa gtggattttc accccataaa
     8401 gtttgaacct gctcagagta caactcaatt ttccccgagg gagttggaaa tcgataatcg
     8461 gagaatgcaa tttcctgaag accgggagca agtattgggc ctttcctgag ctcttcgagc
     8521 gataagttaa actcgcgcag cttactatca aggtacctat caacatgttc atcgcccggg
     8581 ggaagtaaaa tattggataa ctcggcctca ttaaacccta gtttttgtgc aagccaccag
     8641 taaatttcgg tttcgggctt aacctctccg gctggttcaa ccactttttg cttgagctga
     8701 atgtaaggat tccagtacga accaactata tcggattgtt caaacatgtt tttagccggt
     8761 aggataatat cggcttccag agccgtatcg gtcatgaatt gctccaccac cactctgaaa
     8821 tcgagtttcc ggaaggcctc aagaactttg cccgtatttg ggttttgggt tagcggattt
     8881 ccgcgttcta cccaaatcat ttttagttca ggatttttag tggcaagcat gtcctctcct
     8941 aaccgcgcag ttgaaatgga acgacgtata acgccatcgg gtttatcggg cggatagtaa
     9001 cagagtggct ccagtacatc gtcaaaaaca taactttgaa ggttagcgta gtgccagcat
     9061 gcgccggatt tcccaatatt acctgtaatt acactaaggg caagtattgc ccttacagtc
     9121 tgtcccccgt ttgtatatcg ctgcatgcca taacctggta ttaatgtcat tggttttaca
     9181 ttgccaatgt accatgccag cttctcaatt aagctaactg gcacccccga tatattggat
     9241 gcccattgag ccgtaaaggg tttaacatgt tcagcaaact gatcaaaacc atgaacatgg
     9301 agtttgataa aatcactatc aatccatccg ttttgaataa ttatgttggc aacagccaat
     9361 gcaataggtg catcggttga tgggtatggc tgtagcagca aagtagctct ttgggttgat
     9421 tgggttctac gtggatcgat aaccacaagt tccgccccct tatcaagcgc attgttcagg
     9481 ggtatcattt cgtgaacgtt gctctcagct gggtttttcc cccagagaac aattagctta
     9541 gcattctcta aatcccaagg aacattgtgc ttattttcac caagcgttaa gcgcgtagcc
     9601 tccaaacctg caggccagca aagattaccg taaacggttg tagcccctcc gtaaacataa
     9661 cgccagaact ttccgctgaa atcgtttaac aagccggcca tgccgctggc agcgtaaaac
     9721 aaaaccgatt gttgaccgta aacatcgcgg aaatgtatca gcttttcagc aatagtttca
     9781 agagcctcgt tccatgaaat gcgtttaaag gaaccattta cctttttgag tggataaata
     9841 atacgatcgg gtgaatttgc tcgttccaca tagcttaaac ctttaaggca tcccccaaaa
     9901 ggagttgcct tatttaaggg taaatggttg atacctacaa cttttccctt atcaacctgc
     9961 acaacaaggc tacatgtgct gtagcagttt cgtgggcaag cagtgatgaa ctccttcact
    10021 taactttact tttgggtttc ggcaaggtat tgctccttgg aaataaagaa accatccttt
    10081 aggtagtaaa ctgcaccata ctcatggtcg acacgagtgt agaaggtgat tgtgttgttc
    10141 cggttaaaca ctgttcgggt aagtattatt cgtggataac ggataacctc atcggtacgt
    10201 tcacgggtat atcgtttggc aatatcctcg gcagcaagca gggttacaac ctgatcggca
    10261 tcctccttgc caatttgggc gctcattttc tcggccacaa gcttaaggcg taactcagca
    10321 tcgcgcttac gctgctcctc aaggagcaac tttttttgct cctctgctct acggcgttcc
    10381 tcctcctcac gttttagccg ttcggcttcc tgtctagctt tttcctgttc tgccttcaga
    10441 cgttcctcac gtagtttagc ttcggcaata gccctttctt cagcctcttt ctttaacctt
    10501 tcagcctctt gcagttttgc ctcttcctcg gctttacgtt tggcttcctc ggccaatcgt
    10561 tgagcctcag cctccttgcg aatgcgttct tcttccttta aacgagcctc ctcggcagcc
    10621 ttacgtttag cttcttcttc ctgtcgaaca cgctcttgct cctgtttaag cctttcctcc
    10681 tcctgctttc tcaattcttc tttcttacgg gcttcctcct ctgcttttaa cctcgcctct
    10741 tcctcagctt ttttgcgttg ctcttccaat cttttttgtt cctcaagttt tgctttttcc
    10801 tctgccagcc ttcgctcctc ggcttccttt ctcagccttt cctcttcttt aagtctggcc
    10861 tcctcggcag ctttacgttt tgcttcctcc tcctgacgga tacgttcctg ttcttttctc
    10921 agcctttcct cctcctgttt tctgagttcc tctttcttac gagcttcctc ctcagctttt
    10981 aaccgtgctt cctcctcggc ctttttctta agttcctcca ggcgcttttg ctcttccaac
    11041 tttttctttt cctcagcaat gcgtcgctct tcagcctctt tccttagcct ttcctcctcc
    11101 ttttttcttg cctcctcagc agctttgcgc ttagcctcct cttcctcttt acgtttttgg
    11161 gcttcagcta aaatgcgttg tttttcctcc tctgcctttc gggctttttc ttcctcctcc
    11221 ttcttctttt tctcctcagc taactttttg ttttcatttt caatttcttt aagcacttta
    11281 agggagtagt cggtatcgta agtaaagtct cttatatccc ttgcatacat tatctttgca
    11341 acaggattgg catactcctt aataacgtcc tcactctgtt tttcaagaac cacatcaaat
    11401 ccggtcattt gctgaatatc cttacggaat gtttcaggaa cccgggtatc aattaaaacc
    11461 tttttggtga tatagtcagg tttctcaaaa actatgatat actcgaagtt atactcaaga
    11521 ataaaatcaa aaactccttt attgctaacc gatatggttt gaactacatt tccgttttgg
    11581 gttactgtta cctttgaacc ctgtaaatcg ccctttttaa ctattagcct agccgatacc
    11641 tgtaatccat tctgagcaat ggccgatagg caaagtaaaa aaataaccga tactgtaaaa
    11701 aaagcctttg atattattga tttcatgagc gacatattct gcattttcag tccaagcaat
    11761 tgcaaaataa caaaagttct aacaaattaa gtaaaaatat ttgaaagtga tacgaataaa
    11821 ttgactaatc ggttcgacat tttgtaataa tccttgtttt acgttttgta tccgatgcta
    11881 ttatatcgaa gaattcattg gtatacctaa ttagctcatt tctttgcgcc tggggtaaat
    11941 atttaaacgt ggtaatagta tggtttattg tatcctttag gttaaggaaa tagctgaagg
    12001 catcagcaaa ctgctgttcc ggcttacagt agccattgaa gtgtcgctct tttaccgatt
    12061 ttatgggtag gtgctcagcg ggaagcgcat aaggagcatc aacaaagcca gaaaagtcga
    12121 aatcaaaagg aacgggaatg gatggagcaa agggttcggg ttcaattagg gtgatattgt
    12181 gcaatgcctt tattgaccaa tcggtatggc caatcatgta ctgaaaaatg gccatcctat
    12241 tgtagtagta gctattgcag gcgtttgggt gagtgttttt agcttcccta accactcctc
    12301 ctaatcttct tgatagtatt tcaaccggtt caattacaaa ggcatagcaa attgactggt
    12361 tgccatagta tctatctacc atattcaacc ttaaaagcct tactctgtat gaatagggtg
    12421 aaataatctg gtaaagcttg taaacaaggt actcctttag gactaaatct tcgtatttgc
    12481 tagatttttg gcatggtacc accaatttta gtttttcaat accctcaaaa atggtatttg
    12541 aggtctcttt tggtttaaat tttatgagca gcggtggttg ggaacaattt gatgcttttc
    12601 gtcggaaaat tccacgagtt cttatctcaa cgggaatact aatagtagcg ccattttcgt
    12661 tctcatagct taaatatcct ttatggtacg atgggtcttc gcctatatcg gcaaaaactt
    12721 gctgaatgcc aaattcaagt ttaacatcaa gcaatgaagt atcggcaaat acaggttgcc
    12781 catctgctaa attgctaaaa agtagcaata ccaaaaaaat agtatttgat gcaattctgt
    12841 ttagcaccac tcattgaaat ggttctcctg taaaaataca attatttcgg ctactatgct
    12901 gatttttttc tataccgcag tatggctatg cttagaactg caactgccat tagactgatt
    12961 gatagaaaac tatctttcag gtttgtaaaa tcggagcctt ttagaatgag catcctgttt
    13021 accttcataa agtagtatag cgggttgatg gtattaaacc agtttgccca ttttggcatg
    13081 ctctcagttg gggtgaacaa cccacttagc atcaggaaaa caatcatgaa aaagaaagcc
    13141 ataaacatta cctgctgctg attgctagcc gatgttgaga gtatcaaacc taatcccagt
    13201 gctaccaaaa ggtatattgc agcaaaagca aacagaagtg gaatgctgcc tgcaatggtt
    13261 aggttaaata caagcctggc aattagtaga ccaaacgaca gttcaaataa cgcaattatc
    13321 cagaatggaa gtaacttgcc aattacaaac tgatagggct taataggggt aacgttaacc
    13381 tgctcaatgg tgccaagctc tttctcccta acaatattga ttgccgttaa gaacatgcca
    13441 acaatggtaa ctaaaattac aagaatacct ggaaccatgt aaagcttata gttcagttct
    13501 ggattatacc aataccttac gctttgttta ggtttgatat gatttatatt ttgtattgaa
    13561 atgctggatg cgaagttctg aataattccg gtggtataac tctgtgttaa tgctgccgct
    13621 gtaatgttaa tggcattcag cataagctga atatttacgg gtgccccctc aactaggctt
    13681 tccccaaaac gctttgggat taccagtgca gcatcggttt tacctaaatc gattagttca
    13741 acagccctgt caacattgcc gctaacaggt atcaaggtat aaaattttga ttggtcaaaa
    13801 gcgtttatta gcctgcgcga ttccgatgaa ttatcaaaat caactatggt caacctgatg
    13861 tttttcatct cctgtgtagc tgcgttagcc aggatgagca gctgaacgat gggcaatacg
    13921 aatattattg gtagtatagt tttattcctg aaaatctgaa taaactcttt ctgtaaaagt
    13981 attagtatcc ttctcatagc aagctgtatt actccaaacg aactttaaac tttttgatac
    14041 ttagggctat aaaaacagct gtcatggtta gcattaccag cacttcgctt attacgtatt
    14101 gtaagccttg tccttttatc attattgttt ttaatgccga aagaaaccat cgtggtggca
    14161 taatcaggct taaccattgc agaatttttg gcatgttttc aatggggaaa atgaatcccg
    14221 agaggagaat ggtgggcaac atcaatgcaa acattgacat gaacatggca acagcctgac
    14281 tattgctcaa agtggatatt aaaattccta atgatagggc taacactatg aacaacgagc
    14341 taacccccat cagcaaaagg aagtttcccc ttaagggcag atgaaaaact gtgtagccca
    14401 ttaaaatgat taccagaaca ttggtaaatg caatgcctat ataaggaatt actttaccta
    14461 ccacaatctg ccaaggtttt aatggtgaaa ccaacagtat ttccatggta cccatctctt
    14521 tctcacgagt aattgaaata gaagtcatca ttgcagtaac taacatcagt ataagcacca
    14581 tggttcctgg cacaaacatg aatgccgagt tcatttcctc attatacagc attcggcttt
    14641 gaacgtttac cctctccttt tgctggcttg aaccaaaata ctccgcagta aacgaattta
    14701 ctattccttg tgcatacgat gccagaatac ttgctgtatt ggggtctgag gcatcggtta
    14761 caacttgata attggcaaag ttttcatttt gaagcttttg agtgaaattg ttaccaataa
    14821 taagaaccag cttggcatcg ccattcttga aaacttcatt aatttcattt tcgcttttca
    14881 gatatccctt aaagttaaag taccccgatg atgaaatacg ctgcactagc ttttttgagt
    14941 tataatcgtt ggtgtagtca agcacggcgt aattggcatt cttgatttca ctggatataa
    15001 caaagccaaa aattagcagt tgcaggacag gcattacaag cacaataagc agcgttcgcg
    15061 tatcgcgaaa tatgtgctta aattccttgg taataaaatt cgaaaaccta ccctttgcca
    15121 tagctactca atgtttaatt taccatttcg ggatagtagt aagaacaagt cgttcattga
    15181 ttcaactcca tgtgtcgatt ttagttgcgc gggtgcaccc tgagcttcaa ttcttccatc
    15241 aaccatgaca agaacagtat ggcagtactc tgcttcgtcc atgtaatgag tggttaccag
    15301 cacggttgtt cccatagagg agatttgatg aatggtttcc cagaacattc gtcgtccaac
    15361 cgggtcaaca ccgcttgtgg gttcatcgag gaagatgatt gggggcttat gcactaacga
    15421 taccgctagc gaaattcttt gtcgccaccc aaccggcaac gattcaacct tagtgtcttt
    15481 aaaatcgtgc attttgaact cctccacaag ttcaagtgca cgtattctta gctcttttcc
    15541 ccataaccca taaataccac caaaaaatcg tatgttctcc caaaccgtta aatcgctgta
    15601 aagcgaaaac ttttggctca tatagccaat gctggcccta atttgttcag gctgagtgta
    15661 agcatcataa cccgcaacgc taattttccc cgatgtgggt gaaagcagcc cgcagagcat
    15721 cctgattgtg gtagtcttac ctgcaccatt ggctccaaga agtccaaata tttccccttt
    15781 accaacacta aaggttaggt gatttacagc aataaaactt ccaaagcgtt ttacgaggtt
    15841 ttcaacctca atggcatttg tcatttcaca agattttttt ccgagagttg gtaaatgaaa
    15901 taatcctcaa tgcttgcctg tatttgagct acattagctt caactcctac cgacatcaat
    15961 ctatcgtgaa ggctttcagg actttcctca ttgtaattag ttgcgaagtg aatactatcg
    16021 ccaaacatgt aggcggtttc gcatgtactt aaatgcctaa tggcatctaa actactccat
    16081 gcattgttag cctgaatgct aaagagttta tagggaaaca tttcagcaaa cccttcggga
    16141 gtgttagttc ctaaaattcg ccctttctga atcaatgcaa ccctatcgca ctgctttgcc
    16201 tcatccatgt acgctgttga aacaaaaaca gtaataccct gtttcttaat agaatgtaac
    16261 aaatcccata gttcctttct tgaaacagcg tcaactccgg ttgttggctc atcgagtatc
    16321 agcacccttg ggcgatgaat caatgcgcac gaaagggcaa gtttttgttt cataccaccc
    16381 gatagctttc cggcagggcg atttctaaat ggttcaagca tgtgatatac accctttacc
    16441 agttcgtaat tttcctttac tgttgttcca aaaaccccgg caaaaaagtt taagttctct
    16501 tccacggtta aatcggaata aagtgagaaa acagcaggca tatacccaat ttcgcccctt
    16561 atcagtttat actggcttac ggtatccaaa ccaaaaaccg ttacattccc gctatcggga
    16621 actaacagtg ttgtaataat acggattaac gttgattttc ctgctccatc ggggccaatt
    16681 aaaccaaaca tctcaccctc ctcaacgctc agacttacat cggtaagcgc tttcaccttg
    16741 ccgtatgatt ttgatatgtt agttacttgt attgctggca tttagataga gtttagaatt
    16801 aaacgtcagt gttcagcaaa aaataatgtc aggaagaagt agataaaaaa gtaaacccaa
    16861 aaaacataac tgtctatcat tcactacaca tagaacacaa aacaatgcac actaatcttc
    16921 aatcctaacc tccactggca ttcctatctt aaaatagcca tttgggttgg gaactcgaac
    16981 ttttacagca tacacaaggt taaccctttc gttccttgtt tgaataattt ttggagtgaa
    17041 ttcagcttcc gatgaaaccc atgcaatttc gcctttatcc tccttgagtt taccatctga
    17101 ggtatcggtt aaaattgtca ccttacggcc aattgaaaaa tcgggaagtt gagtttcatc
    17161 aatataaacc ctcgcgtaaa ccgtatcgag cgtaccaatt ttgcaaaccg gttttccggt
    17221 aataaccagc tctccctggc tcacatagcg cgatattact gtccctgaaa caggtgaaac
    17281 catgtggcac cgccacaata aatccagggc ctgggcaatt tgagcatcta cagcgtcaat
    17341 ttctgccaac acccctgagc gttgcgcatc aaacgttgct ttttgctttt cggcaatttt
    17401 gaactttccc tcaatatcat cgagctgacg cagtgttgct gcgctatcgg ctaataattt
    17461 ttttacccta tcaagttcct ttttcaggac gttcagctgc tcatcctgaa cggcaatttg
    17521 agcatcaata ccttgcagct ttgccttggc agcctttagc tgggcattaa gctgctttag
    17581 tttaaggact tgcggtaaag tatcaacaat tccaataagt tgatttttct ctactttata
    17641 tccctcgtcg attttaagtt ccagaagttt accgcccact tctgcggaaa tggttacatc
    17701 gttcgattca atgttcccgt aagcatcagc ttttccatta tccctactac atgaaataac
    17761 aattgcagtg ggaagaatta cgttgataag atttttccaa ttcattggta taatttttta
    17821 atcgttaata ctcatgccaa gtgtgaaata tagcctcaca acctcctgtg agagctttat
    17881 tttatgtaag tccatatcaa tttttgctct aagcaagctg ttaaagtcgg acaagtaaac
    17941 cgatgatgtt atggttccgt tttttagctg cgatgctgat gcctttacag acttttctag
    18001 cagctcaact attttttcat cggtttgtat ttgcctttgt aacgattgta tctgcgctaa
    18061 cgctgatgaa acctgcgcac tacatccttc atcgtaagca ttgcgcctat attgcaaaat
    18121 gttctgttgg attttcaggg tctgcctctc gcgcgatgtg ctattccaat cccatatact
    18181 ccatgataat cgggctccaa ccaaggcaaa ggaatcaaac tcattggaaa gcatgttaag
    18241 tcccggtttg ccatagccaa cccgtgcaaa tgcacttagc ttaggcattc gcctgctagt
    18301 caaggcacct gctgctgttg acaggtagcc gttctgcagg tcaaaaagtt tataatcggg
    18361 gcgtgaacaa acatcctggt ttggtatagc taatgagggt ataatccctt gggcactctc
    18421 atcaattttt actcccgtaa gtgagtaaat tgattctgtc aaactatacc gttgcgaaag
    18481 gtttgcatca atgctttgct ccaacctaag cctctcagcc tttaaagcgt ttgctgatga
    18541 ttccagtacc accccagcat ttacaccgga ctcaagctcc tctattcgcc tatcgagctc
    18601 cacggccata gctttaagct gcctgattgc catatcagta gaaagtattc caaagtaaag
    18661 cgaagccacc ctttcctttt gggtgtaaag ttcgctttca accttgttct gctcaagttt
    18721 acgcgattca tcttcaagct gatgcatcga ttttgttgca cctccatccc aaatcagctg
    18781 gctaacatcg atagtcaatt tatagttgtc gttatcgggt gtagggattg aaatgcccgg
    18841 catgggaatg ttaactttgg ttacatccga ttgccaggtg gcctcggcat tcaattctac
    18901 ttttggtaag tagtttacct tgatattcga cttcctaagc cagtaaaccg agtcggttgc
    18961 cgcactaacc ctgtaaagct tattccactg ctccatgctt ttaagtaagc ccggcagagt
    19021 aaccgtgttc tggctcttta gcatataggt tgaagccagt aaagtaatgg taagcgaaat
    19081 aattcttgcc tttctcattg ttaaaatcat tttaatcttc cttttagaaa gtaaatcata
    19141 aagtcggcaa catacctttt acgttcactc aaaaactttt ggtaatcgcc ctcgttgcca
    19201 ttaaaaagca cctgttcaat gattggttta ccggcaaacg ggaaaataca tgagctaaca
    19261 gtagttacca taaggtgaag cacctcgttc acgcttaatc ccaattcatg ggataaaacc
    19321 ttcatcacat cttttagttt ttcggcattg atgcccgatt cagtaatgaa ctgtgcaatc
    19381 atttcgggat ttcgattagc ctcgcgcagc acaaaagcag gtagaaaggg atttctaagg
    19441 aaaagttcga tatactcttc cacaaaatgc cgaatttttt gnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn natttttttt ggtgtcatac aatattaacc atttggttaa
    19621 accatacggc aaatatctga cacatttttt tatgctccaa attttttagt ctctttttta
    19681 accatttggt taattttttt tgtatgcggc taattatcta tatttgtgat tagctaataa
    19741 aggttattat ggatattcat acgccaattg taattaacgg aataaatatt aaaaaccggg
    19801 ttgccttgcc acccattgtt aactttggat ggtccgactc cgatggaaag gtaagcagca
    19861 agcatattgc acactacgaa agaattgcta ggggtggcac cggtctaatt attgttgaag
    19921 ccacctgtgt gaatcccact ggtagaattt tcagctacca gttaggcata tgggacgaca
    19981 accacattga ggggcttgcc agaattgctg aggctgtcca caagcacgac gccaaggttc
    20041 ttattcagct tcaccatgca ggacttacca cacggaaagc tgttgccgaa aaggcctttg
    20101 gtccctccgt tgacccggaa aacgaccgtt ccgtagcctt aaccaaagaa caagtaagtg
    20161 aactggtaga aaattttgta agcgcagcca aaagggcaca cctagcgggc tttgatggtg
    20221 ttgaactaca cggtgcccat ggttacctgc ttaaccagtt cgcctcttct gccattaacc
    20281 accgaaccga tgagtatggt ggaaactctg agaacaggtt acgatttgca aggcaaatca
    20341 ttgaaggtat caaggaagtt acaccctcaa catttattat tggttaccgc atggctggtt
    20401 gttcccccac cctgaacgat ggaattgaaa ttgccaaact gctccaggca tacggcgttc
    20461 attaccttca tgtatcgcac ggtggcgaaa agggtattaa ccccacagtt ccctcaaatt
    20521 ttgactacaa ccatgtggtt tacatgggaa cagaggtaaa aaagtttgta gatgttcctg
    20581 taatggtggt taaccaaata aaatcacctg aaagggctaa taagttggtc acacacggct
    20641 tagctgatat ggtagccatt ggacgtgaca tgttaaccga ccccgattgg gtaaccaagg
    20701 caaaaacagg cgaggctata aacctttgta ttgattgtca gccgcgctgt aaacgctaca
    20761 ccaaacctga atcgtgccca ctggcggagt aggtagaggg aaggataaag gataaaggat
    20821 aaaggataaa ggataaagga taaaggataa aggttaaagg ttaaaggttg acccgtccta
    20881 tgttagcggg aatagaagta aaaggtgttt cgtgttttgt gttttatgtt tggtgttatg
    20941 aaaaatccaa atatttagta atcagtcata tataagcatt tcgcattgtt ttttgtcatt
    21001 atcaatcttc aactatcaac catcaactat caactatcaa ctatcaacta tcaactatca
    21061 accgtcaacc atcaactaat gtgcgccatc cgtgttccat ttcattttgc gaacaacgga
    21121 cagcgcactg cagatatttt cctagccctt taagctaatc tctgttcaag ttaaattccc
    21181 ctgctttaag tcgctttaaa tagccttcgg cctcggcttt cattactggg aataaaagca
    21241 gtagcgctat caggtttgga atgctcataa aggtgataac catatccaca aagtgccaaa
    21301 ccaaatcgat accccaaact gaaccaaaga atacaaatag ggtaaaaacg tagtagtaag
    21361 gtttaatggc cttatcgcca aaaatgtact gtgcagttct ggttccgtag tatgaccagc
    21421 taatgatggt agagaacgca aaaagtagca ggctaatggc caccatgtga cgcgctaaaa
    21481 agccaatgcc aattttaccc aagccctcct gaaaagcagc aactgtcatc cctacccctt
    21541 taacggcacc atctaatcgc catacgccgg ttaaaacaat aaccaatgca gtaagggtac
    21601 aaattacaat ggtatcgatg aaaggttcaa gcgaagccac aaacccctca cgcatggggt
    21661 atggagtttt tgcagcagca tgcgccatag gcgctgagcc ctgtccggcc tcattggaaa
    21721 acaaaccccg gcgaacaccc catatcatag tcatgaaaaa ggttgaaccc acaaaaccac
    21781 caactgctgc ttggcccgta aaagcacttt ttacaatcaa ccataaagct gaaggtatat
    21841 cggcatagtg aacacctatg acaagaattg cagaaccaaa gtagataaca gccattaaag
    21901 gcacaagctt agaggtaaca ttggcaatgc ggcgcaatcc gccaactaca attaaaaaca
    21961 ctaacgctga aatggcaata ccggtgtaaa gaacagggat attatagcta gtttttaaaa
    22021 catcggagat tgaattggct tgagccatat tgccagttcc taatgaacaa agaatcccag
    22081 caaaggcaaa gaaaacagcc aaacgcttag cccaaacacc cagcttatcc ttaagaccat
    22141 gttcaatgta gtacatagga ccgcccgaaa cagtgccatc ggcattaatt ttacggaact
    22201 tttgcgccaa tgtacattca accattttaa gcatcatgcc aaagaagccg gtaacccaaa
    22261 gccagaacac agccccaggc ccgccaaaat agattgcaag ggtaactcca acaatatttc
    22321 cggttccaac agtggctgaa agcgccgtgg taagagcctt gtaatggctt acatcgccta
    22381 catcatcgtg cttgctatat ttccctcgaa ccaagtcgat agcatgctta aaatggctta
    22441 cctgtaaaaa tttaagtctg attgagtagt aaatgcctgt gggaaccagc agggctaata
    22501 tcaagtaacc cccaacatac tcgttgtaaa gctgaataac gtaatcgaaa ctttccagaa
    22561 aagtgttcat ggttttggtt tggtttaggt tatcgcacaa aaatagaaaa aacaaaaacc
    22621 tgtcaaagga tagacaggtt tattgttaat aagtttaaaa atttttgtca tttagatttg
    22681 atttggtaga tttaaattca atgtcgttta gttaacaaaa atatacaagc ctgtcatcct
    22741 gagcgcagcg aaggatcact aatcgacccc atgagacgtt tcgcttagct caacatggca
    22801 atagcttcac gaacaacaaa caacgcttcc agccccgtag gggcgcaata tttgtaacac
    22861 aatagttact ccctcatgca tagcgatgca cgccatggca ctccaaagag caagggtgaa
    22921 acattgcatc gcaaaggaaa aacattgcaa agagtgattt tcacgttctt tcttgtcttg
    22981 atacaagaaa gaaccaaaga a
//
LOCUS       DAOOWJ010000035        22009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2366_length_22009_cov_7.947800, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000035 DAOOWJ010000000
VERSION     DAOOWJ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 22009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22009
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2366_length_22009_cov_7.947800"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLW22_05140"
     CDS             <1..719
                     /locus_tag="PLW22_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74125.1"
                     /translation="DENGNDISSSKIDFSYSFQNDYQNPNMLIIGIIINKMKEGKWFI
                     YNSKEKIGFDLYKNDSLIRSKINKSYIKINDKLINNTLFIPYSLYACDKIIIKSDVSI
                     EDYPFLSVFMKNVWRPIESAMGILEDSSSVVPDNKPMYIDGIEGINKTISQNLIVNKK
                     YVTECKNFGWLYYEIIIDENGNVIDQNILKSPDSIITEIALESLKSLNTFKPAYFQGK
                     PIKSKFSSRILFREPRIVKP"
     gene            964..1323
                     /locus_tag="PLW22_05145"
     CDS             964..1323
                     /locus_tag="PLW22_05145"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOU74126.1"
                     /translation="MRLSIKILSILFVLLGFSGISAAQKFYEAEDVKDAQVKFFIVDS
                     PEQADLHFCIIYEASEITKPGVMMEVETPKEADITLIFVDNEKDADIKVWLVDTPAEV
                     KWINESKKKLLSVGGLK"
     gene            1393..2562
                     /locus_tag="PLW22_05150"
     CDS             1393..2562
                     /locus_tag="PLW22_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOU74127.1"
                     /translation="MNKNSRYDQRGVSASKDDVHKAISNLDKGLFPKAFCKVVPDYLT
                     GDPDYCLVMHADGAGTKSSLAYVYWRETGDMSVWKGIAQDAIVMNIDDLLCVGITDNI
                     LLSSTIGRNKNLIPGEVVSAIIDGTEEFCQTMRGMGINIVLTGGETADVGDLVRTVIV
                     DSTVTARIKRSQVISNSNIQDGDVIIGLASFGQASYEKEYNGGMGSNGLTSARHDVFG
                     HYLAPKYPESYDPQVPESLVYSGNLKLEQPVEGTGLTAGKLVLSPTRTYAPIIKAILD
                     NYRPVIHGMVHCSGGAQTKVMNFVNNLHVVKDNLFPVPPLFRIIQEQSNTPWQEMYKV
                     FNMGHRFEIYVYPEFADDIIAIAKEFKVDAQIVGYCTEATSNKLTIKSPYGEFHY"
     gene            2610..3701
                     /gene="prfA"
                     /locus_tag="PLW22_05155"
     CDS             2610..3701
                     /gene="prfA"
                     /locus_tag="PLW22_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOU74128.1"
                     /translation="MADNTILRKLEGIRHKFEEIGQQITDPAVMADMKKFVALNKEYR
                     QLEPIVEAYEKYKNVLSNIESSRQILANEKDEELREMAKMELETLTQEQDQLEEEIKI
                     LLLPADPQDSKNAVMEIRAGTGGDEASIFAGDLFRMYTKFFEKRGWKYEVTSFSEGTV
                     GGYKEIIMNVTGNGVYGVLKYESGVHRVQRVPQTETQGRVHTSAATVAVLPEADEFDI
                     DLKMEDIRKDTFCSSGPGGQSVNTTYSAIRLTHIPTGIVVQCQDQKSQIKNFEKALTE
                     LRTRLYNMEYQKYLDEIASKRKTMVSTGDRSAKIRTYNYPQGRVTDHRINLTLYNLSA
                     IMDGDLQEVIDKLQLAENAERLKAAAAEE"
     gene            3714..4547
                     /gene="pyrF"
                     /locus_tag="PLW22_05160"
     CDS             3714..4547
                     /gene="pyrF"
                     /locus_tag="PLW22_05160"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOU74129.1"
                     /translation="MTAQELFEQIKKKRSFLCVGLDTELTKIPEHLLTKDDPMFEFNR
                     KIIEATHDLAIAYKLNLAFYEVHGAAGWNSLVKTVKYLKTHHPDVLVIADAKRGDIAN
                     TARMYAKAYFHSLGCDAVTVSPYLGQDSVVPYLQYDGKWTILLAITSNESFGDFQLVH
                     NKQTGNLLFEDVVLKSMHWGSDANMMFVVGATHPEYFAAVRKHAPHHFLLVPGVGEQG
                     GSLQKVAENGMNDRCGLIVNSSRGIIYADITGNFTSEARKRALKLQQEMEVLLKQNGL
                     I"
     gene            complement(4554..5669)
                     /locus_tag="PLW22_05165"
     CDS             complement(4554..5669)
                     /locus_tag="PLW22_05165"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOU74130.1"
                     /translation="MKNRWKVLLITTGVLLGILSCSRDSGNIVIGIDDIGKHDRVLIL
                     VHPTENNIKTFNYLIDSKILPIPSVTKVIGVYHTSEKYDYSKSVEYIKKNKLENFKLL
                     PVKEPITETAIYRVNECTDVFARLTKVSDGVIFTGGPDIPPSIYGEKTSLLTQITDPA
                     RHYFEVSFMFHLIGGSKDTLFQPLLDAKPLMPVLGICLGMQTMNVAAGGTLVQDIPSE
                     IYGIESVEDALIQQTNSLHRNYNANYALDDKLLWGNFHQISILDEPLKSICQGQPWVL
                     SSHHQGVERLGRNLKIIATSIDGNVVEAIVHTKYPNVIGLQFHPEPTLLYNNDEFLRF
                     LPNQPSRLTYLNMYSGEAGEKFHLNFWSWFGKKVIEQ"
     gene            complement(5688..7145)
                     /locus_tag="PLW22_05170"
     CDS             complement(5688..7145)
                     /locus_tag="PLW22_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOU74131.1"
                     /translation="MNRITIIASALATFYCSVLSFAQQVDSNMLKDHVYYLASDSLMG
                     RGFGTEGGRMAAKYITDKFKEVGLTPWQGQYLHSFISSSMMLKTEGANIIGWVEGNDP
                     VLKNEYIVLGAHYDHVAYKIVNGEKVVYNGADDNASGVASIIEIGRWLVLNRDKLKRS
                     VIIIAFDGEEAGLIGSSHLVNNKVIPTDRVKFMFSLDMVGMLQKYGGIDLVGNKTLSD
                     GDEFFVQLASKHGIQVKKSGRSVEMQTDTQPFGKIGIPAVHVFTSTVSPYHKPEDDAD
                     KLDYVGMAKIASFVADATLQLSTTDSVKPDARFAAKTLGSDKFSAGYQVGIGNTYHDY
                     RDEFYNSKEGFSFNTGLSFTIGLSRYIDLQVGALYQTTGTEHRYGHFRTHEVMAPASI
                     LLYMGGKSKQMLSPNLYFIGGGYYSYRFAGKVGNGSFDMDNNIQKDNYGFQVGVGFSI
                     MNVNFQFVRQVAVLSLEKDRNVFPSTLMFNMGFRF"
     gene            7426..8022
                     /locus_tag="PLW22_05175"
     CDS             7426..8022
                     /locus_tag="PLW22_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74132.1"
                     /translation="MKKTLLLGFAALTMVAMLSSCGKVPQEKIDAANAAIDSAKAAEA
                     DVYLAADFAAVQDSMSAVMADIEVQKSKLFKKFGPASEKLDRILGMAQQLKVNAVAKK
                     EEVKKEVEGLMTEVATMMEENGKLMKKAPRGKEGAAVLEQMKAEMATIEATVNEAKGL
                     YDAGKYMDASNKMKAAKESATKINTELNEAIAKVKGRK"
     gene            8092..9234
                     /locus_tag="PLW22_05180"
     CDS             8092..9234
                     /locus_tag="PLW22_05180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOU74133.1"
                     /translation="MKPILKYSLITLATLSVIGGGFALFYYSIPEPPKGDIDYALASL
                     HQASRSNAKRYSKQKYLKAKAYFDSAMKHWNIQNERFILSRDYQKVSYFAKKSAEMAK
                     SATSNSKELSKNLVISVKDKIDNLTALVSNMNNLFQRLPLPDEFRKKISKGKMLIHEA
                     EVAFKKGDYLESSEKIKLAEDLIMDSYKKTLDDLEDYFNSQPYWNRLVKSAISNTAKN
                     NSMAIIVDKFARKLYIYQNGTKKYEFDAEFGKNWMGRKRLKGDKATPEGIYLVKSKIP
                     ANKTKYFRALLLNYPNSEDIENFNKEKASGRIPKHAQIGGLIEIHGDGGKGTDWTDGC
                     IALDNNDMLQVYKIVEIGTPVVIVGSIADFNTIAMSLNLKRNQIKG"
     gene            9237..9953
                     /locus_tag="PLW22_05185"
     CDS             9237..9953
                     /locus_tag="PLW22_05185"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOU74134.1"
                     /translation="MNSNEDKIDFNPEINPKLKRLGFVMLLINIILSGFIILYFYLTY
                     LSQPMAELVVKNRKLTFVSDSIQDDKVDVKKLQKEVASLKQQFQNLTPAQPYIVVNTT
                     YNRFRLYKNRKQVREGFCSTGSYTLLKSHDKRQWIFHTPKGMYKVLGKVTNPVWRRPD
                     WSYVEEGLPIPSANDPSRWEAGVLGDYALSIGDGYLIHGTIYKRFLGMPVTHGCIRMN
                     DEDLEAVYKTLDLGSKVYIY"
     gene            9965..10750
                     /locus_tag="PLW22_05190"
     CDS             9965..10750
                     /locus_tag="PLW22_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74135.1"
                     /translation="MRKPIKITLYTTAILVVILAVAYTILKVIAFQKAINNIEQHIAK
                     ELNKRKKTVEWVSEPDSTLKKLLVDKAYYNALITASKTDSIFLAINIPDSTVSLMIKG
                     VVAHKAKAEHIKVSPLFSAINPPLLIQGLSVPLTVSKHTSTIPKVPLMIKIAPRDTSE
                     YTPDVIPDTTDLDPVNFIIELKPNILLYFYESNPSQSSDGFNQFKFDLRERTWTVVQD
                     VKRMLKLKTPVYKPYIRFKLGKKDAKVIYRAIPNQGQVVLRLE"
     gene            complement(10782..10855)
                     /locus_tag="PLW22_05195"
     tRNA            complement(10782..10855)
                     /locus_tag="PLW22_05195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10819..10821),aa:Pro,seq:cgg)
     gene            10961..12232
                     /locus_tag="PLW22_05200"
     CDS             10961..12232
                     /locus_tag="PLW22_05200"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOU74136.1"
                     /translation="MDESLLHFQWRYQHYTLNGLSTTKGDTVEVIDPGLYNTDSGPDF
                     SNAKVRIGDTLWVGNVEMHIKSSDWFTHNHQTDNAYNNVILHVVVEHDRTAVTSKGTE
                     VQTLVIPYPNKLVHSHENLIKSTEWIPCASKIGVVDSLLINSTIDRMQVERLENKSKR
                     VAELVSQCNGSWEEAFYRDLCRSFGLKSNALPFQLLAQSLPQKVLAKHKNSAFQLEAL
                     LFGQSGLLDKAKNDSYVISLKQEYTYLKNKFSLTPIEPSLWKFMRLRPASFPTIRIAL
                     LAMLAHKSSGLFSQLMEVKNIDNVIKFFAVEASDYWTNHYVFGEESISKKKRLGEETI
                     RLITINTIIPFMYSYGRSRAKADLEANALTILQQMPPENNTIVRNFGALGIKALSAFD
                     SQALVHLKTFYCETRKCIYCPIGVSILRKQL"
     gene            12259..13263
                     /locus_tag="PLW22_05205"
     CDS             12259..13263
                     /locus_tag="PLW22_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HOU74137.1"
                     /translation="MEQKGGRTLALAITILLSIITIGTVGYIHIEDFTFTEAIYMTVI
                     TIGTVGFKEVRPLSPEGMWFTITLIIISIGFFAYAATFLAKYFSEQILSRYSNKKIME
                     KMIAKMQNHVIVVGYGRNGSQAVEELLAHNIPVVVIENRPKVIEYIQQNPKIAYVDGD
                     ATLDITLQKAGVDKAKALITALANDADNLFVVITAREMNPKLKIISRASAVTNDRKLK
                     LAGADNVILPDKVGGQKMAKLVIQPDILEFVENIMLKRTGNISLFEISCRKILEVGAK
                     SLDELQIREKTGVNVIGVRNPIGEYILNPEPNHILTHDSLLFVLGTTEQVEKLKDLIS
                     "
     gene            13275..14162
                     /locus_tag="PLW22_05210"
     CDS             13275..14162
                     /locus_tag="PLW22_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU74138.1"
                     /translation="MKILVTGANGLVGKNIAKALHATNQVEVFATSLKKFQLEGVTAF
                     TSNLLNADINHMVDDIKPEIVVHCAALSSPDACEVDRYACHRMNVELTKRIVSACVDY
                     NVHLVFLSTDFIFDGSKGNYTEMDAPNPVNYYGESKLEAEKLIQASGVVNTIVRTSLV
                     YGVESKLSRPNIITRVFDNLSKNKQYKVPSDQIRTPTYAPDLASAISQICLNRVTGIY
                     NISGDQIISVADFAILAAKAFDLNHELLLQVNSKDLAEAAPRPKNTSLVIGKAKNDLN
                     FKPTPIEMALGKIKTTLNS"
     gene            14325..14597
                     /gene="rpsO"
                     /locus_tag="PLW22_05215"
     CDS             14325..14597
                     /gene="rpsO"
                     /locus_tag="PLW22_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOU74139.1"
                     /translation="MNYLTAEQKKEIFEKYGKSNTDTGSPESQIALFSYRINHLTEHL
                     KVHRKDYGTQRALLKLVGKRRRLLDYLKENDIERYRTIVKELNLRK"
     gene            14737..16863
                     /gene="pnp"
                     /locus_tag="PLW22_05220"
     CDS             14737..16863
                     /gene="pnp"
                     /locus_tag="PLW22_05220"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOU74140.1"
                     /translation="MQKVVKKTIDLGDGRTITIETGKLAKQAHGSVVLTMGKTMLLAT
                     VVSAQEAKEDVDFMPLSVEYKEKFAASGRFPGGFLKRESKPSDYEILVARLVDRALRP
                     LFPDDYHAETFVTVNLISAEKEIMPDALAGLAASAALAVSDVPFHGPISEVRVARING
                     KLKINPTFKEVEEADIDMIVGATYDNILMVEGELKEVSEADMLEAIKFAHEAIKPQCL
                     AQMELAKELGVVKRTYCHETNDEELKAKVHEFCYDKVYQVAKSMLPKQERTAAFEAIK
                     QAFIETIPEEEREAKQFMVNRYYHAVEKEAMRNMILNEGVRLDGRKTHEIRPIWCEVD
                     YLPMAHGSAIFTRGETQSLTTVTLGTKLDEKIIDEVLMQGTEKFTLHYNFPPFSTGEA
                     KPSRGVSRREIGHGNLAHRALKNMVPDAPENPYAIRVVSDILESNGSSSMATVCAGTL
                     ALMDAGVQIKKPVSGIAMGLITEKGSDRYAVLSDILGDEDHLGDMDFKVAGTRDGITA
                     TQMDIKVDGLSYEVLAKALKQAHDGRMHILDIMMQTISKPRPDFKPHAPRIEQFNIPR
                     ETIGAVIGPGGKVIQEIQRETQTTITIEEINDHGVVSVFAESAESRDAAVKWIKGIVA
                     VPEVGEIYKGKVKSIMPFGAFVEILPGKDGLLHISEIEWRRLEKVEEVLKEGDMVDVK
                     LIEIDKKTGKLKLSRKVLLPKPEKNK"
     gene            16994..19108
                     /locus_tag="PLW22_05225"
     CDS             16994..19108
                     /locus_tag="PLW22_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU74141.1"
                     /translation="MKKIFILMLLATPILITMSCNTAKVKYPKTKQVDVVDEYFGVKV
                     ADPYRWLEDDKAPEVAEWVKQQNEVTFNYLNAIPFRNKIKERLTTIWNYTSMGAPFKA
                     GGRYFFFKKEGLQNQSVLYMMENLEAEPKVILDPNTFSEDGTVSLSNVSVSRDGKYLA
                     YSINDGGSDWQKIKVIKLPGGEQLPDEISWVKFSSIAWLNDGFFYSRYDEPKGGSELS
                     NINQNHKVFYHKVGTPVETDKLIFSNSQYPLRNYSAQTTDDEKYLIIYESESTYGNSI
                     YVKNLKDPRANFSKITTGFEFEYSVLDHVDDNLIVLTNYKAPKYKLVKINVNSMDIGN
                     WRDILPERKDVLKECALINKKIIANYIVDAKSQAEVYNLDGSKITDIELPTLGTISGI
                     SGDINDSIAFYSFSSFTVPPTVVKYNVNTNTSTQFFSPNIDFKFDDYETKQVFYTSKD
                     GTLVPMFIVHKKGIKLDGNNPTLLYGYGGFNVSLLPYFSTSRLFWLEQGGIFAMANLR
                     GGGEYGENWYRAGTKLNKQNVFDDFIAAAEYLIKEKYTSPKKLAIQGGSNGGLLIGAV
                     TNQRPDLFKVAIPQVGVMDMLRYHNFTIGWAWANDYGTSNDSIQFLNLYSYSPLHNIK
                     PKTNYPAILVTTADHDDRVVPAHSFKYIATMQEKNPNPKNPALIRIQTRAGHGAGMPT
                     SMVIDEYADIYAFIMYNMGMQY"
     gene            19212..19841
                     /locus_tag="PLW22_05230"
     CDS             19212..19841
                     /locus_tag="PLW22_05230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU74142.1"
                     /translation="MHYLVIEGNIGAGKTTLATMLAKELNAKLILEQFADNPFLPKFY
                     KNPERYSFPLELSFLAERYSQLNNELRSASLFHSLTIADYYFMKSLIFAQNTLSGDEL
                     QLYKQLFSIIYSTLPKPDLYVYLHLPTNQLLKNIRKRGRDYEQTITAEYLNGITEGYF
                     DFFKQHPEYTFLVIDTSKIDFVANRDDFLKIKSTIFDQHYKKGVNLVSL"
     gene            19958..21658
                     /locus_tag="PLW22_05235"
     CDS             19958..21658
                     /locus_tag="PLW22_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74143.1"
                     /translation="MKMKGLKLSIIALAGLLLAGCGGVEKMKELPAGFTLKVTPNPVE
                     VHANKVKATFEGTIPPKYFNKKATVEITPVVVYKGGELALPSKVLQGEDVQANNQVIN
                     YEAGGSWKHDVEFDYKDEMFSSEIELRFVITYKDKKVPFDAPQKVGIGTMATYKLVDI
                     DVKPVMLNDKFVRVTPEEKEAQINFVIQKADIRKSELTKDEIKMLNDFLLAAAQAKNK
                     EIKELKIAAYASPDGPEKLNDKLSQDRGKNTDKYLADVFKKAKVEGVTPTVEFTAEDW
                     DGFQKAVQASDIEDKELIIRVLSMYSDPVVREKEIKNLSKVYKVLAEKILPQLRRSRM
                     VAKVDVIGYSDDTLKYMVENNAYDSLNVEEMLYAATLVNDVNKKIEVYMKAGEKFNCA
                     RGYNNAAYYYILNKDVANAKAALDKIPADQKIAAVINNRGCVAMLEGNFDEAEKLFAD
                     AADAGSAVKYNLGIIAILKGQYPAAAEYLAGSDSFNQGLALLLAGKVDAAKNTFNKVE
                     TAKGYYGRAIVGARLAEEATVLDNLRTAFGKDASLKDRAKEDVEFRTFFQNEAFKALF
                     "
     gene            complement(21767..>22009)
                     /locus_tag="PLW22_05240"
     CDS             complement(21767..>22009)
                     /locus_tag="PLW22_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU74144.1"
                     /translation="YVIISVEQVEHSVLLNQFYPKKGQKYALVFGNEVKGVGQDVVDI
                     SDVCIEIPQLGTKHSFNISVSAGIVLWDFFVKVMVD"
BASE COUNT         6906 a         4665 c         4487 g         5951 t
ORIGIN      
        1 atgatgaaaa tgggaatgat atctcttcga gtaaaataga cttttcatat tcattccaaa
       61 acgattatca aaatccaaat atgttaatca ttggaattat tattaataaa atgaaagaag
      121 gtaaatggtt tatatataat agtaaagaaa aaataggttt tgatctttac aagaatgaca
      181 gtttaattag atcaaaaata aataaatctt atataaagat aaatgataaa ttgataaata
      241 atactctttt tatcccatac tccttatatg cttgcgataa aataattatt aaaagcgacg
      301 taagtataga ggactatcct tttttaagcg tttttatgaa aaatgtttgg cggccaattg
      361 aatcagcaat gggtattctc gaagattctt caagtgttgt acctgataac aagccaatgt
      421 atatagatgg tattgaagga ataaataaaa ctatttcaca gaatttaatt gttaataaaa
      481 aatatgtaac tgaatgcaaa aattttggat ggttatacta tgaaataatt attgatgaaa
      541 atggcaacgt tatagaccaa aatatactaa aaagccctga cagtataatt acagaaatag
      601 cattagaatc gctcaaatca ttaaatactt ttaaacccgc atattttcaa gggaaaccta
      661 taaaatcgaa atttagttca aggatcctat ttcgtgaacc aaggattgtt aaaccctaga
      721 gaattgaata aaaaaaacac cttgtcggct tataaattca tcacttgatc gtttagttaa
      781 cattagcaaa tagttcattg atgtcaattt taattatttg tttcatttta ctttggttta
      841 ataaactggc actaaatttg tcatcacttt attacttttc aaacattttg ttagttttgc
      901 attgaaccaa ctattcaatg taacctaatg aaagcattat tagtataatg attttgttat
      961 acaatgcgac tttcaataaa aatattatcc atactatttg ttctgcttgg cttcagtggc
     1021 atatcagcag ctcaaaagtt ttatgaagct gaagatgtca aggatgccca ggtgaaattc
     1081 tttattgttg atagtccaga gcaggcagac ctgcatttct gcatcatata cgaggcttcc
     1141 gaaataacta aaccgggtgt aatgatggag gttgaaaccc ccaaggaggc cgatatcact
     1201 ctgatatttg ttgataatga gaaagatgcc gatattaagg tttggcttgt agacactcca
     1261 gccgaggtga aatggattaa tgagtcgaaa aagaaattgc tttctgttgg gggattaaag
     1321 taattcccct ctttcatctt caagttggtt gtaacgtaaa tataggttac ctttgcagtt
     1381 aaaacgcacc ccatgaacaa aaattcccgt tacgaccaac gcggcgtttc cgcatcgaaa
     1441 gatgatgtcc ataaggcaat tagcaacctg gacaaggggc tcttcccaaa agcattctgc
     1501 aaggttgttc ccgattacct tacaggcgac cccgattact gcttggtgat gcatgctgac
     1561 ggggccggta ctaaatcttc gcttgcctat gtatattggc gcgaaaccgg cgatatgtcc
     1621 gtttggaaag gcattgcaca ggacgctatt gtaatgaaca tcgacgattt gctttgcgtg
     1681 gggattaccg ataatatttt gctatcatca accattggcc gtaacaagaa cctgattccg
     1741 ggcgaagtgg tttcggccat cattgatggc acggaggagt tctgccaaac catgcgcggt
     1801 atgggaatta acattgtgct taccggtggc gaaacagccg atgtggggga tttagtgcga
     1861 acagttattg tagattctac tgttactgcc cgcataaaaa ggagccaggt tatttccaat
     1921 agcaatattc aggatggcga tgtaatcatt ggtctggcat cgttcggcca agcctcatac
     1981 gaaaaggaat ataacggagg gatgggtagc aatggactaa cctcggcacg ccacgatgta
     2041 ttcgggcact accttgcccc aaagtatccg gaaagctacg accctcaggt acctgaaagt
     2101 ttggtttaca gcggcaacct taaacttgag caacccgttg agggaactgg ccttacggca
     2161 ggcaaactgg ttctatcccc tacccgaacc tatgcaccta tcattaaagc catactagat
     2221 aattaccggc ctgtaataca tggaatggtt cactgttcgg gaggcgcaca aactaaggtg
     2281 atgaattttg tgaacaacct tcacgtggtt aaggataacc tcttccctgt accaccgcta
     2341 ttccgtataa ttcaagaaca gtcaaatact ccctggcagg aaatgtataa agtattcaac
     2401 atggggcacc gattcgaaat ttatgtttac cctgagtttg ccgatgacat tattgcaatt
     2461 gctaaagagt ttaaggtaga tgcccaaatt gttggatact gtactgaggc tacttcaaat
     2521 aaattaacca ttaagtcgcc ttacggtgag tttcattact aactcaaaaa gagatttttt
     2581 gcttaccttt gcgccaataa atttttgata tggcagataa tactatactg cgtaagcttg
     2641 aaggaataag gcataagttc gaggagattg gccagcagat aaccgatcct gctgtaatgg
     2701 ccgacatgaa aaagtttgtc gcacttaaca aggaatacag gcagttggag cccattgttg
     2761 aggcttacga aaaatacaaa aacgttctga gcaacataga atcgtccagg caaatactgg
     2821 ctaacgagaa ggacgaggaa ctccgtgaga tggctaagat ggagctagaa acccttaccc
     2881 aggaacaaga tcagctcgag gaggaaatca aaattctact tctccccgcc gatcctcaag
     2941 actccaaaaa tgctgtaatg gaaattcgtg ctggcaccgg tggcgacgag gcaagtattt
     3001 ttgccggtga cctattccgc atgtacacaa aattctttga aaaaagagga tggaagtacg
     3061 aggttaccag tttctccgag ggtacggttg gaggttataa ggaaattatc atgaatgtta
     3121 ccggtaatgg cgtttacggt gtgcttaaat atgagtcggg ggttcatcgg gttcagcgtg
     3181 tacctcaaac cgagactcag ggtagagtac atacttccgc tgctaccgtt gccgtacttc
     3241 ctgaggccga tgagtttgat atcgacctta aaatggaaga tattcgtaag gataccttct
     3301 gctccagtgg gcctggcggg caaagcgtta acaccaccta ctcagccatt agattaaccc
     3361 atatacccac cggcattgtg gttcaatgcc aggatcaaaa atcacagata aagaattttg
     3421 aaaaagccct tacggaactc cgtacccgcc tttacaacat ggaataccaa aaatacctcg
     3481 atgaaattgc atcgaagcgt aaaacgatgg tttccactgg cgaccgttcc gcaaaaatca
     3541 gaacatacaa ctacccgcaa gggcgtgtaa ccgatcatcg aataaatctc accctgtata
     3601 acctgtcggc cataatggat ggcgatttac aggaagtaat agataaacta cagcttgccg
     3661 aaaatgctga aaggttaaaa gcagctgctg ctgaagaata aaaaacaatg cctatgacag
     3721 cccaagaact atttgaacag ataaaaaaga aacgaagttt cctgtgtgtt gggctcgata
     3781 ctgaactcac taaaatccct gagcatttgc tcaccaagga tgatccaatg tttgagttca
     3841 accgtaaaat tattgaggcc acccacgatt tagccatcgc atacaagtta aatcttgctt
     3901 tctatgaggt tcatggtgca gcgggctgga atagcttggt gaaaaccgta aaatacctta
     3961 aaacgcatca ccccgatgtt ctggtaattg ccgatgctaa gcggggcgac attgcaaaca
     4021 cagccagaat gtatgctaaa gcgtactttc atagcttagg ctgcgatgcc gttacagtat
     4081 cgccctatct tggccaggac tcggttgtac cctacctaca gtacgacggc aaatggacaa
     4141 tccttttggc aatcacatca aacgagagct ttggcgattt tcagctggta cacaataagc
     4201 aaaccggcaa cctcctcttt gaggatgtag tgcttaaatc gatgcactgg ggcagcgatg
     4261 ccaatatgat gtttgttgtt ggagcaactc accctgaata ctttgctgct gttcgtaagc
     4321 atgcacctca ccacttcctg ctagttcctg gggttggcga gcaaggcgga agccttcaaa
     4381 aagtagcaga aaacggcatg aacgaccgtt gcggcctgat tgtaaattca tcgcggggaa
     4441 tcatttacgc cgatattacc gggaatttta cctctgaggc tcgtaagcgc gcccttaaac
     4501 tccagcagga aatggaagtg ttgctcaaac agaatggatt aatatagttc cacttactgc
     4561 tctataacct ttttaccaaa ccacgaccaa aaattaagat gaaatttctc gccagcttca
     4621 ccggagtaca tatttaagta tgtaagcctc gatggctggt tgggcaaaaa gcgtaagaac
     4681 tcatcgttat tatataggag tgtaggctct ggatggaact gcagtccaat tacgtttggg
     4741 tattttgtat gaacaatagc ctctaccaca ttaccatcaa ttgaggtggc aataattttt
     4801 aaattccttc ccagtcgttc caccccctga tggtgactgc ttaataccca gggctgtccc
     4861 tggcaaatgg attttagagg ttcatcaagg atactgatct ggtggaaatt cccccaaagg
     4921 agtttatcgt ccaaagcata attggcattg taattcctgt gcaagctgtt ggtttgctgt
     4981 atgagggcat cctcaacact ttcaattccg taaatctcgg aagggatatc ctgcactaaa
     5041 gtgccacctg ctgcaacgtt catggtttgc attccaaggc aaatgcctag gacaggcatt
     5101 aaaggcttgg catcgagcag gggctgaaat aaagtatcct ttgagccccc aattaaatga
     5161 aacatgaacg acacctcaaa gtaatggcga gccggatcgg ttatttgggt taatagcgat
     5221 gttttctctc cataaattga tggggggata tcggggccac ctgtaaagat tacaccatcg
     5281 ctcaccttgg ttaaccgagc aaaaacatca gtacattcgt ttacccggta aattgcggtt
     5341 tcggttattg gttccttaac gggcaacagc ttaaaattct ccagcttatt cttcttaatg
     5401 tactcaaccg attttgagta atcgtatttt tcagaggtgt gatacacacc aataactttt
     5461 gtcaccgaag gtattggcaa tatcttgcta tcaattaaat agttaaatgt tttgatattg
     5521 ttctcggttg ggtgtactag aattagtacc ctatcgtgtt tcccaatgtc atcaatccct
     5581 ataacaatat taccagaatc tctactacag gataggatgc ctagcaatac tcctgtagta
     5641 atcagtaaaa ctttccatct gttcttcata actatagtat taaggtacta aaacctaaaa
     5701 cccatgttaa acataagtgt actgggaaaa acatttctat ccttttctaa tgacaacact
     5761 gcaacctgac gaacaaactg gaaattaaca ttcataatgc tgaaccccac gccaacctga
     5821 aagccataat tatccttttg aatgttattg tccatgtcaa aacttccatt accaaccttt
     5881 ccggcaaatc ggtaggagta gtatcctcct cctataaagt acagatttgg cgataacatt
     5941 tgcttacttt tcccacccat gtaaagaagg atactggctg gagccataac ctcatgggtt
     6001 ctgaaatgac cataccgatg ttctgttcca gttgtttggt aaagtgcacc aacctgtaaa
     6061 tcgatatacc tacttaatcc aattgtgaag gataaccctg tgttaaaaga aaaaccttcc
     6121 tttgagttgt aaaactcatc cctgtaatcg tgataagtat taccaatgcc tacttggtat
     6181 ccagccgaaa atttatcgct acccaaagtt tttgcagcaa accttgcatc gggtttaacg
     6241 ctatcagtag ttgaaagctg tagggttgca tcggctacaa aacttgcaat tttcgccatt
     6301 cccacatagt caagcttatc ggcatcgtct tcgggtttat ggtaaggcga aaccgtagag
     6361 gtaaacacat gcacggcagg tattcctatt ttcccaaacg gttgagtgtc ggtttgcatt
     6421 tcaacacttc gtcccgattt tttcacttga ataccatgct ttgaagccaa ctgaacgaaa
     6481 aattcatcgc catcgctaag cgttttattt ccaaccaggt cgatacctcc atacttttgc
     6541 agcattccaa ccatgtcgag gctaaacata aactttaccc tgtcggtagg gataacctta
     6601 ttgtttacaa ggtgacttga acctattaat ccggcctcct ccccatcgaa agcaatgatt
     6661 ataacagagc gtttaagctt atcgcggtta agtacaagcc agcgcccaat ttcgataatt
     6721 gaagcaaccc ccgaggcgtt atcgtcagct ccattgtaaa ccactttttc gccgtttact
     6781 attttatagg ccacatggtc gtaatgcgct ccaagtacta tgtattcatt ttttagtacc
     6841 ggatcattcc cctcaaccca accaataata tttgcccctt cagtcttgag catcatactg
     6901 gaactgataa atgagtgtag gtactgccct tgccaaggag ttagtccaac ctctttgaac
     6961 ttatcagtga tgtattttgc agccatgcgc cccccttcag tcccaaaacc cctacccata
     7021 agtgaatcgg acgcaagata gtaaacatga tcctttaaca tattcgaatc aacttgctga
     7081 gcaaatgata aaacgctgca ataaaaggta gcaagtgcgc ttgcaataat tgtaatccta
     7141 ttcattttac aagacctttg aaaatttatt gtaatgttac aaaatatttt aaaatgctga
     7201 ccctataaag cagtattatt aacctaaaag tttactctag tcaatagata tgcttttaac
     7261 atttttttat atttttttta acttgattat caatacttta caatattttt tatagtttag
     7321 ataaaaaaaa atacgcgaat acgaaacaat gaattgaaca accccataca tttgcattga
     7381 aaattaaact ttaatgttta actaacaaac ccaaacattt cgattatgaa aaagacactt
     7441 ttacttggtt ttgcagcctt aactatggtt gctatgcttt caagctgtgg caaagtgccc
     7501 caggaaaaga ttgatgctgc taacgccgct attgattcgg ctaaagctgc tgaagctgat
     7561 gtttatcttg ctgctgattt cgctgctgtt caggattcaa tgagcgctgt tatggctgac
     7621 attgaagttc aaaagtcaaa attattcaag aaatttggcc cagctagcga aaaactcgat
     7681 cgtattcttg gcatggctca gcagctaaag gttaacgctg ttgctaagaa agaagaagtt
     7741 aagaaagagg tagaaggcct tatgaccgaa gttgccacca tgatggaaga aaatggcaag
     7801 ctcatgaaga aggctcctcg tggtaaggaa ggtgctgctg ttcttgagca aatgaaagct
     7861 gaaatggcta ccatcgaagc tactgttaac gaagctaagg gtctttacga tgctggcaag
     7921 tacatggatg cttccaacaa gatgaaagct gctaaagaaa gcgctaccaa gattaacacc
     7981 gaacttaacg aggctattgc taaggttaag ggcagaaagt aatttattta catgctataa
     8041 aatccccggc cgttttttta ccggggattt ttttttgata tttgtacccg tatgaaaccg
     8101 attttaaagt acagcttaat tacccttgca acgctatcag ttataggtgg cggatttgca
     8161 ctattttact attccatacc cgaaccgcct aaaggcgata tcgattatgc cttagcctcg
     8221 ctacaccagg catcgcggtc aaatgccaaa cgttactcaa aacaaaagta cctaaaggca
     8281 aaggcatact ttgattcggc catgaagcat tggaatatcc aaaatgaacg cttcatttta
     8341 agccgcgact accagaaggt atcgtacttt gccaagaaat cggccgaaat ggctaaaagc
     8401 gcaacaagta actccaagga actttcaaaa aacttggtta tatcggttaa ggataaaatt
     8461 gacaacctaa ccgcccttgt ctcgaacatg aacaatcttt ttcagaggtt acccctgcct
     8521 gatgagtttc gcaaaaaaat ctcaaaaggt aaaatgttaa tccatgaggc agaggtagca
     8581 tttaagaaag gcgattacct tgagtcgtcg gaaaaaatca agttggctga ggatttaatt
     8641 atggattcct acaaaaaaac cctcgacgac cttgaagatt actttaactc acaaccctac
     8701 tggaaccgac ttgtaaaaag cgctattagc aatacagcca aaaacaactc catggctatt
     8761 attgtggata aatttgcccg aaaactctat atctaccaaa acggtacaaa aaagtatgaa
     8821 tttgatgccg agtttggcaa gaattggatg gggcgtaaac ggctgaaagg cgacaaggct
     8881 actcccgagg gaatttatct ggttaaaagc aaaatacctg caaataaaac caagtatttt
     8941 agggcgttgc tactcaacta ccctaattca gaggatattg aaaatttcaa caaggaaaaa
     9001 gcttcaggcc gaatccccaa gcacgctcaa attggcggac taattgagat ccatggcgat
     9061 ggcggcaaag gaaccgattg gactgatgga tgtattgctc ttgacaacaa cgatatgcta
     9121 caggtgtata agattgttga aattggcact ccggtagtta ttgttggatc aattgccgac
     9181 ttcaacacca tagccatgag cctgaacctt aaacgtaatc aaataaaggg ttaactatga
     9241 atagcaatga agataaaata gatttcaacc ccgaaataaa ccctaagcta aaacggttgg
     9301 gctttgtaat gctgcttatc aatatcatcc tttcaggctt catcattctt tatttttatc
     9361 tcacctatct ttcgcaaccc atggcggaat tagtggttaa gaacagaaag ttaacctttg
     9421 tatccgactc catacaggac gataaggttg atgtaaagaa acttcaaaag gaagtggcct
     9481 cattaaagca acagttccaa aaccttacgc ccgcacaacc atacattgtg gttaacacaa
     9541 cgtacaatcg cttcaggcta tacaaaaacc gcaaacaggt tcgtgaagga ttttgtagta
     9601 ctggcagcta tacactacta aagtcgcacg ataaacgtca atggattttt cataccccaa
     9661 agggtatgta taaggtgtta gggaaggtaa caaatcctgt atggcgtcgc ccggactggt
     9721 catacgttga ggaagggtta cccattccat cggccaacga cccatcgcgt tgggaagctg
     9781 gcgttttggg cgactatgcc ctgagcattg gcgatgggta cctgatccat ggaacaatat
     9841 acaagcgatt cctggggatg cctgtaacgc acggatgtat cagaatgaat gacgaggatc
     9901 ttgaagcagt ttacaaaacc ctagatctgg gatcgaaggt ttacatttac taatgatcgt
     9961 ttttatgcga aaacctataa aaatcaccct ctacaccaca gccatactag ttgtaatact
    10021 ggctgttgca tataccattc ttaaagtaat agcattccag aaagccataa acaacattga
    10081 gcaacacatt gcaaaagagc tcaataaacg aaagaaaaca gttgaatggg ttagtgaacc
    10141 cgactcaact ttaaaaaaat tattggtgga taaggcttac tacaacgcat tgattaccgc
    10201 ttccaaaact gactcaatat ttttagccat taatattccg gacagtaccg tttcgttaat
    10261 gattaaaggg gttgttgctc ataaagcaaa agcagagcat attaaagtaa gtcctttgtt
    10321 ttctgctatt aatccaccct tgcttattca aggattgtcg gttccattaa cagtatcgaa
    10381 gcatacctca accatcccaa aagtaccatt aatgatcaaa attgccccac gcgacacctc
    10441 agagtacacg cccgatgtta tacctgatac aaccgacctt gaccctgtta actttattat
    10501 tgagctaaaa ccaaacattt tactttactt ctacgagtca aacccttcgc aatcgtcgga
    10561 tgggtttaat cagttcaagt tcgatcttag agagcgcacg tggactgttg ttcaggatgt
    10621 taaacgaatg ctaaaactta aaacaccggt ttacaagccc tacatcagat ttaagcttgg
    10681 caagaaagat gcaaaggtga tataccgggc aattcccaac cagggacaag tagttttacg
    10741 attagaataa aaaaagagct gacattaaat caactctaaa gtcggggtgg ggagactcga
    10801 actcccggcc tctacgtccc gaacgtagcg cgctagccaa ctgcgccaca ccccgtttgc
    10861 gcttgcaaaa ttactacaat tttactttcg ccataatagt attgcccagt tttttggatt
    10921 tttttaactt gtacatccaa atacaattca ccctttttaa atggacgaat cgcttttaca
    10981 cttccagtgg cgctaccagc actataccct aaatgggctc agcactacaa aaggcgatac
    11041 ggttgaggtt atcgatcccg gattgtacaa taccgactca ggccccgatt tttccaacgc
    11101 aaaagttcga attggtgata ctctttgggt aggtaatgtt gaaatgcaca tcaaatcgtc
    11161 cgactggttt acccataacc atcaaaccga caacgcttac aacaatgtaa ttctacacgt
    11221 agttgttgag cacgatagaa ccgcagtaac atcgaaagga acagaagttc agacactagt
    11281 cattccttac cccaacaaac tggtgcatag ccatgaaaac ttgatcaaat cgaccgaatg
    11341 gatcccatgc gccagtaaaa ttggggttgt tgattcattg ctaattaact caaccattga
    11401 tcgaatgcaa gttgaaaggt tagaaaacaa gagtaaacgt gtagctgagc tggtaagcca
    11461 atgcaatgga agttgggagg aggctttcta tcgcgattta tgccgttcat ttgggctaaa
    11521 aagtaacgca cttccctttc agctactggc tcaaagctta ccgcaaaaag tattggcaaa
    11581 acataaaaac agtgcatttc agcttgaggc cctactattc ggtcaatcgg gcttgctcga
    11641 taaagcaaaa aacgatagct atgtaatttc tttgaaacag gagtatacct atctgaaaaa
    11701 caaattctca cttacaccca tagagccaag cctctggaag tttatgaggc tcaggccagc
    11761 atcgttccca accattagaa ttgctctttt ggcaatgctt gcacacaaat cgtcgggtct
    11821 cttctctcag cttatggagg tgaaaaatat tgataatgta attaaattct ttgctgttga
    11881 agcatcggat tactggacta atcactacgt ttttggtgag gaatcaattt ccaagaaaaa
    11941 acgtttgggc gaggaaacca ttaggttaat aaccataaac acaattatcc ctttcatgta
    12001 tagctatggt cggagcaggg caaaagctga ccttgaggcc aacgcactaa ctatactcca
    12061 acaaatgcca cccgagaata atacaattgt cagaaacttt ggtgccttgg gcatcaaagc
    12121 attgtcggct tttgactctc aggcattggt tcacctaaaa accttttact gcgagacccg
    12181 aaaatgtatt tactgcccta ttggtgttag tattttacga aaacaattat aattttaaag
    12241 cgtcgtaaaa attgctgaat ggagcaaaaa ggaggtagaa cattagcgct tgcaatcaca
    12301 attctcctaa gtataataac cataggaaca gttggttata tacacattga agactttaca
    12361 ttcactgaag caatctacat gaccgtaatt acaatcggta cagtaggatt taaggaggtt
    12421 cggccgcttt caccagaggg aatgtggttt accataacac taatcatcat aagcattgga
    12481 ttctttgcct atgcagctac cttcttagcc aaatactttt cggaacaaat actttcccga
    12541 tactcaaata agaagatcat ggagaagatg attgccaaaa tgcaaaacca tgttatagtg
    12601 gtgggatacg gacgtaatgg ttcacaggca gttgaagaac tgcttgcgca taacatccct
    12661 gttgttgtta ttgagaacag gcccaaagta attgagtata tacagcaaaa ccccaaaata
    12721 gcatatgttg atggcgatgc cacccttgat atcaccttgc agaaagcagg agtggataaa
    12781 gctaaagcgc taattacggc tctggcgaac gatgccgaca acctttttgt agtaataacc
    12841 gccagggaaa tgaatccaaa gcttaaaatc attagcagag catcagccgt tacaaacgat
    12901 agaaaattaa agcttgctgg cgccgacaat gtgatactgc ccgacaaggt tggaggacaa
    12961 aaaatggcaa aactggttat tcaaccggat attcttgagt ttgttgagaa tatcatgcta
    13021 aaacgaaccg ggaacattag cctttttgaa atatcatgta ggaaaattct cgaggttggg
    13081 gcaaaatcgt tggatgaact acaaataagg gaaaaaacag gcgttaatgt aattggcgtt
    13141 aggaatccca ttggcgaata cattttaaac cctgaaccta atcacatact tacccacgac
    13201 tctttactgt ttgttctggg aactacagag caagttgaaa agctaaaaga tctaatctcc
    13261 taaaacattt taaaatgaaa atccttgtta ctggggcaaa tggccttgta gggaaaaaca
    13321 tagcaaaagc tttacatgcc actaaccagg ttgaagtttt tgccacatcg ctcaaaaagt
    13381 ttcagctcga gggggtaacc gcttttacat cgaacctgtt gaatgctgac atcaaccaca
    13441 tggttgatga tattaaaccc gagattgttg ttcactgcgc tgccctttcc agcccggatg
    13501 cctgtgaagt tgacaggtac gcctgtcaca ggatgaacgt tgagctaacc aaacgcattg
    13561 tatcggcctg cgttgactat aatgttcacc ttgtgttcct ctcaaccgat ttcatttttg
    13621 acggctctaa aggcaactac acagagatgg atgcacctaa cccagtaaat tactatgggg
    13681 aatcgaaact tgaagcagaa aagttaatac aggcctcggg tgtggtcaat acaatagtaa
    13741 gaacctcttt ggtctacggg gttgagtcca aactttcgcg tccaaacatt attacccgtg
    13801 tattcgataa cctcagcaaa aacaagcagt acaaagtacc aagcgatcaa atccgcaccc
    13861 caacctatgc tccggattta gcttctgcca tctcccagat ctgtttgaac agggttacag
    13921 gcatttataa tatttctggc gaccaaataa tttcagttgc tgattttgcc atacttgctg
    13981 caaaagcctt tgacctgaac catgaattgc tgctgcaagt aaattcaaaa gacttagccg
    14041 aggctgctcc cagaccaaaa aacacctcgc tggttatagg caaggcaaaa aacgatttaa
    14101 acttcaaacc cactcccatt gaaatggcac tgggaaaaat taaaactacg ctaaatagtt
    14161 aaaatatact ataaacatgt taatattctg tatgtcataa tattaagcaa ttaaaaatta
    14221 atagctgttc gaaaaaaaag tatcaattgt aacgatattg aaaaaaaggt tttatctttg
    14281 cacctcaaat taagtagtaa atctacccac taattatcag tgttatgaac tatttgacgg
    14341 ccgaacagaa aaaagagatt tttgagaagt acgggaagtc caataccgat accggctcac
    14401 ctgagagcca gatcgcgtta ttctcctacc gtatcaacca tttaactgag cacctaaagg
    14461 ttcaccgcaa ggattatggt acccagcgcg ctctgttaaa gttggtgggt aagcgcagga
    14521 ggcttttgga ttacttaaaa gaaaacgata ttgaaagata tcgaacaata gtaaaggaat
    14581 taaatctcag aaagtaagaa atcaaaaaag gcaatttcta aaattgcctt tttttaatca
    14641 ctatcataac ctccagagga tgggaatcct ttggggttta attgtttaaa caaaggcagt
    14701 attttattgt tgattttatc tatataaatt ttaattatgc agaaagttgt aaaaaagaca
    14761 atcgatttgg gcgatggccg aaccattacc atcgaaaccg gtaaactggc caaacaagcc
    14821 catggctcgg ttgtgctaac aatgggtaaa accatgcttc tcgctacagt tgtaagcgct
    14881 caggaagcaa aagaagatgt tgatttcatg ccgcttagcg tggagtacaa ggagaaattt
    14941 gctgcaagcg gccgtttccc aggaggtttc ttaaaacgcg aatcaaaacc atcggactat
    15001 gaaattctgg tggcccgttt ggttgaccgt gcattacgtc cccttttccc tgacgattac
    15061 catgccgaaa cctttgttac cgttaacctg atttccgctg agaaggaaat tatgcccgac
    15121 gcacttgccg gattggcagc atcggctgcg cttgctgttt ccgacgttcc tttccatggc
    15181 ccaatctcag aggttagagt ggctcgcatt aacggtaagc taaaaattaa ccctaccttt
    15241 aaggaagttg aggaggccga tatcgatatg attgttggcg ccacctacga taatattctg
    15301 atggttgaag gcgaactgaa agaggtgtcg gaagccgata tgctggaagc aattaagttt
    15361 gcccatgagg ccattaaacc ccagtgtttg gctcaaatgg agctcgccaa ggaacttggc
    15421 gtagttaaac gcacctactg ccatgaaacc aacgatgagg aactcaaggc aaaggttcat
    15481 gagttttgct acgacaaggt ttaccaggta gccaaatcga tgctgcccaa gcaagaacgc
    15541 acggcagctt ttgaagccat taagcaagcc tttattgaaa ccattccgga agaggagcgc
    15601 gaagcaaagc agttcatggt gaaccgctac taccatgcgg tagaaaaaga agccatgcgc
    15661 aacatgatac ttaacgaggg tgttcgcctc gatggtcgca aaacccacga aatacgccct
    15721 atctggtgcg aggttgatta tttgcccatg gcacatggat cagccatttt cacccgtggc
    15781 gaaacacaat cgcttaccac tgtaaccctt ggtaccaagc tcgacgaaaa aattattgat
    15841 gaggttctga tgcagggaac tgagaagttt acactgcact acaacttccc acctttctcc
    15901 actggcgaag ccaaaccctc aagaggtgtg agccgcaggg aaattggaca cggaaatctt
    15961 gcccacaggg cgttaaagaa catggtgccc gatgctccag aaaacccata cgcaattcgt
    16021 gttgtgagcg atatacttga atcgaatggt tcatcatcaa tggccactgt ctgcgcaggc
    16081 accctggcgc tgatggatgc aggagttcag attaagaaac cagtatcggg tatagcaatg
    16141 ggtcttatca ctgagaaagg atcggatagg tatgccgtac tttccgacat tcttggcgat
    16201 gaggaccacc ttggtgatat ggactttaag gtggcaggaa cccgcgatgg tattacagcc
    16261 acccaaatgg atataaaggt tgatggccta tcgtacgaag tacttgctaa agctctaaag
    16321 caagcccacg atggacgtat gcacatcctt gatatcatga tgcaaaccat ttccaaaccc
    16381 cgtcccgact ttaaacccca cgccccccgt attgagcagt tcaacatacc tcgcgaaacc
    16441 attggtgccg ttattggccc tggtggtaag gtaattcagg aaattcagcg cgaaacccaa
    16501 accactatca ccattgagga aattaatgat cacggtgtag ttagtgtatt tgccgagagt
    16561 gctgaaagcc gcgatgctgc cgttaaatgg attaaaggaa ttgttgctgt tcccgaggta
    16621 ggcgagattt acaagggtaa ggttaagtcc attatgccct ttggcgcatt tgttgaaata
    16681 cttcccggca aggatgggct gcttcacata tcggaaattg agtggcgtag gctagagaag
    16741 gttgaagagg tgcttaagga aggcgatatg gttgatgtta agcttattga aatcgacaaa
    16801 aaaaccggaa agctgaaact ctcacgaaaa gttttgctgc caaaaccgga gaaaaacaaa
    16861 tagtaaaagc agggtaaccc ctgctttttt tatactttca aatattgtga ttgctttttt
    16921 tcagaaatta gtgcaaggtt tgcataattt tattgtattt ttgttagtat atcaaaacaa
    16981 aattttgaaa gcgatgaaaa agatattcat attgatgttg ttagccacac caattttgat
    17041 tacaatgtcg tgtaacaccg caaaagttaa atatcccaaa accaagcagg tagatgtagt
    17101 tgacgagtat tttggagtaa aagtggccga tccctaccgt tggcttgagg atgacaaagc
    17161 acccgaggtg gctgaatggg ttaagcagca gaatgaggtg actttcaact accttaatgc
    17221 aattcctttc cgcaacaaaa ttaaagaaag gcttaccacc atttggaact atacctcaat
    17281 gggggcccct tttaaagcag gcggcaggta tttctttttc aagaaagagg gacttcagaa
    17341 ccaaagcgtc ctttacatga tggaaaacct tgaggctgag cctaaggtaa ttcttgatcc
    17401 aaatacgttt agtgaggatg gaaccgtatc gctatcgaat gttagcgttt cgcgcgatgg
    17461 caagtacctt gcctattcca ttaacgatgg cggttcggac tggcaaaaga taaaagtgat
    17521 taaactgccc ggcggggagc aacttcctga cgaaatcagt tgggtgaaat tttcatccat
    17581 tgcatggcta aacgatggct tcttctatag ccgctacgac gaacccaagg gtggctcgga
    17641 actttccaat atcaaccaaa accataaagt tttttaccac aaggtaggaa caccagtgga
    17701 aaccgacaag ctaattttct caaatagcca atacccctta cgaaactact ccgcacaaac
    17761 caccgatgac gaaaaatatc tcatcattta tgaatcggaa tcaacctacg gtaattccat
    17821 ctatgtaaaa aacctaaaag accccagggc aaacttttca aagattacca caggctttga
    17881 gtttgagtac agcgttcttg accatgttga cgacaacctc attgttttaa ccaactacaa
    17941 ggctcccaag tataagctgg taaaaatcaa tgtgaacagc atggatattg gcaactggcg
    18001 cgacattcta cctgaacgga aagatgtact gaaggaatgt gccctaatca acaaaaaaat
    18061 aattgccaac tacattgttg atgctaaaag ccaggccgag gtatacaacc tggacggaag
    18121 taaaattacg gacattgaac ttccaaccct tggcacaata tcagggataa gcggcgatat
    18181 aaatgattct attgcctttt attcattctc atcgtttaca gttcccccaa ccgttgtaaa
    18241 gtacaacgta aacaccaata cttcgacaca attctttagc cctaacatcg actttaagtt
    18301 tgatgactac gaaaccaagc aggtatttta cactagcaag gatggcactc ttgttccaat
    18361 gtttattgtc cacaaaaagg gcattaagct cgacgggaat aaccctaccc tgctttacgg
    18421 ctacggtggc ttcaatgtaa gcctactacc ctactttagc acatcgcgct tattctggct
    18481 agagcagggt ggcatatttg ccatggcaaa cctaaggggc ggtggcgaat acggtgagaa
    18541 ctggtatcgt gcgggcacaa agctaaacaa gcaaaacgtt ttcgacgact ttattgcagc
    18601 agccgagtac ttaatcaaag aaaagtacac ctcaccaaaa aaacttgcca tacaaggggg
    18661 gtcaaacggt ggcttgctga ttggtgcagt aaccaatcaa cgtcccgatt tattcaaggt
    18721 agccatccct caggttggcg taatggatat gcttaggtac cataacttta ccataggctg
    18781 ggcttgggct aacgattacg gcactagtaa cgactctatt cagttcctta acctttacag
    18841 ctactccccg ctccataata tcaaacctaa aaccaattac ccggccatac tcgtaaccac
    18901 tgccgaccac gacgataggg ttgtgcctgc gcattcgttc aagtacattg ctaccatgca
    18961 ggagaaaaac cctaacccca aaaatcctgc cctgatacgc attcaaactc gtgccggaca
    19021 cggagcaggt atgccaacct ctatggttat agatgagtac gcagatattt acgcgtttat
    19081 aatgtataac atgggaatgc aatactagag ttgataaatt tttaaagggg ttggggtatt
    19141 acgctccaac ccttttgttt ttatacacca aaaaatataa cttagccagg aacaaacgcc
    19201 tttgaaaaac tatgcattac cttgtaattg aaggaaatat cggtgcaggc aaaacaaccc
    19261 ttgcaaccat gctggcaaaa gaacttaatg ccaaactaat cctggaacaa tttgccgata
    19321 acccctttct tcccaaattc tataaaaacc ctgaacggta tagtttcccg cttgaactct
    19381 cattcctggc agagcgatac tcacagctca ataacgagtt acgttccgct tcccttttcc
    19441 actcattaac aattgcggat tactacttca tgaaatcgct aatttttgct cagaacacac
    19501 taagcggcga tgaacttcag ctttacaagc aactattttc cattatttac agcactttgc
    19561 caaaacccga cctatatgta tacctacatt tacctacaaa ccagttgctt aaaaacattc
    19621 gaaaaagagg acgcgattac gaacaaacca taaccgctga atacctgaac ggaataaccg
    19681 aaggatattt cgattttttc aaacaacatc ctgaatacac tttcttagta atcgatactt
    19741 caaaaattga ctttgtggca aaccgcgatg attttttaaa aatcaaatcg acgatttttg
    19801 accaacatta caaaaaaggt gttaacttgg tgtcgctgta agacaaatgt ttatttttgt
    19861 tccatgaatt tgataaattg gaatttaagt agtatgtttg tatttactaa attgagtgct
    19921 tacaattaag cagaattaat aactaaccaa cttttatatg aaaatgaaag gtttaaaact
    19981 atcgattatt gccttggccg gtctgctttt ggccggttgc ggcggggttg agaaaatgaa
    20041 agaattacct gccggtttta ctttaaaggt aaccccaaac cccgtagaag tacatgctaa
    20101 caaagtaaag gctacttttg aaggtactat tcctccaaag tacttcaaca agaaggccac
    20161 tgttgaaatt acccccgtgg tggtttacaa gggtggcgag ttagctcttc cctcaaaggt
    20221 tttacaaggt gaagacgttc aggccaacaa ccaggttatt aactacgaag ctggtggtag
    20281 ctggaaacat gatgtagagt ttgattacaa ggacgagatg ttcagctctg aaattgaact
    20341 ccgttttgtt attacctaca aggacaaaaa agttcctttc gatgctcctc aaaaggttgg
    20401 cattggtaca atggctacct acaagctggt tgacattgat gttaagcctg taatgctaaa
    20461 cgacaagttt gtacgcgtta ctcctgagga aaaggaagca caaattaatt tcgtaatcca
    20521 aaaggctgac atccgcaagt cggagttaac caaggatgaa atcaagatgt taaacgattt
    20581 cctacttgct gctgctcagg ctaagaacaa ggagattaaa gagcttaaga ttgctgctta
    20641 cgcttcaccc gatggccctg aaaaactaaa cgacaagctg tcgcaggatc gcggaaagaa
    20701 caccgacaag taccttgctg atgtgttcaa gaaagccaaa gtagaaggcg ttaccccaac
    20761 tgttgaattt acagctgaag actgggatgg tttccagaaa gctgttcagg cttccgatat
    20821 cgaagacaaa gaacttatca ttcgcgttct ttccatgtac tccgatccag tagtacgtga
    20881 aaaggaaatc aagaaccttt caaaggttta caaggtattg gctgaaaaga ttctcccaca
    20941 gctccgtcgt tcaagaatgg ttgccaaggt tgatgttatt ggctactccg acgataccct
    21001 aaagtacatg gttgaaaaca acgcatacga cagccttaac gttgaggaaa tgctttacgc
    21061 tgctacactt gtaaacgatg ttaacaagaa gattgaagtt tacatgaaag ccggcgaaaa
    21121 gttcaactgc gctcgcggtt acaacaatgc tgcttactac tacattctga acaaggatgt
    21181 tgctaatgcc aaggctgctc tggataagat tcctgccgat cagaagattg ctgccgttat
    21241 caacaaccgt ggttgcgtag ctatgcttga aggcaacttt gatgaggctg aaaagctatt
    21301 tgccgatgca gctgatgctg gtagcgctgt taagtacaac ctcggtatta ttgcaattct
    21361 taaaggacag tatcctgcag ctgctgaata ccttgctggt agcgattcat tcaaccaggg
    21421 tcttgctctt ctattagcag gaaaggttga tgctgccaag aacaccttta acaaggtaga
    21481 aacagctaag ggttactatg gccgtgcaat tgttggcgct cgccttgctg aagaagcaac
    21541 tgtactcgat aacctacgca ctgcttttgg taaggacgct tcactaaaag atcgcgctaa
    21601 ggaagatgtt gaattccgta cattcttcca gaacgaagct tttaaagcat tattctaaga
    21661 taaatacatt actaagaaac ggggctgcac atgcagcccc gtttttttat tgcaacaaat
    21721 aatacctgaa gttcagcaga cactaaacta gtggaaaagt taaatatcag tcgaccatta
    21781 ctttaacaaa aaaatcccaa agcactatac cagccgagac cgaaatgtta aaggagtgtt
    21841 tggtgcctag ctgtggaatc tcaatgcaaa catctgaaat atctaccaca tcctgtccta
    21901 cacctttaac ttcgtttccg aataccaatg cgtacttctg cccttttttc ggataaaact
    21961 ggttaagcag caccgaatgc tccacctgtt ctactgaaat aatcacata
//
LOCUS       DAOOWJ010000036        21783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2390_length_21783_cov_8.302099, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000036 DAOOWJ010000000
VERSION     DAOOWJ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 21783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21783
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_21783_cov_8.302099"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PLW22_05245"
     CDS             <1..1120
                     /locus_tag="PLW22_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU74145.1"
                     /translation="PYCYVVPHEAVSFYGKDFGHNPVGTGPFYLKLWRDGEKLVLRKN
                     PNYFEVDELGNRLPYLEAVSISFIPDKQSEFLEFVNGKIDFLSGVNAASKDELLTRSG
                     KLNPKYSAKVKMITGPYLNTEYIGILVDSTKLPSNQRFLLDERVRKAIALAFDKEKMM
                     LYLRSNLGYPAYGGFVPSGMPGFTDVNYGLAYNPLQAMQLLEQAGYSSKNALPEITVS
                     TTDDYLDLFEYIQNQLSLVGIKLNIDVLPGAAYREMLANGKLPVFRGSWIADYPDPEN
                     YLACFLTDNFAPNGPNYSHFSSVQFEMLYRKAMSLTSYDQRLPYYHSLDSIVTSKAIV
                     IPLYYDKVVRFTSARVDGIGVNPMNFLVLKKVKVKSIK"
     gene            1117..1974
                     /locus_tag="PLW22_05250"
     CDS             1117..1974
                     /locus_tag="PLW22_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74146.1"
                     /translation="MIIIADSGATKTDWRIIEHGKPIASFETIGLSPYFNRQEDFLSA
                     LNQNYPKQINPEQVKEIFFYGSGCGLHERGQDVARYLGIFFSNAKIHAVTDALGAARA
                     LFKRQTGIVVILGTGSNIAYYDGERLNNRTPSLGFVLGDEGSGAYMGRLLLRAYHYKT
                     LDVGLAQKLEQQYNVELSHVLNMVYSAPRPSAYLASFVPFILENIHNQHIYSIVKESF
                     VALYRYHFAVFENLSDLSIGVTGSVGCLFSQVLDSVGEEYGFKISQYLRYPIVELINY
                     HTQNAQNTP"
     gene            complement(1937..4417)
                     /locus_tag="PLW22_05255"
     CDS             complement(1937..4417)
                     /locus_tag="PLW22_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74147.1"
                     /translation="MDLHKQLIEAVEKRFGKKISYQRDCKTLSEKVFEHTHEYISPAT
                     LRRLFGFLKTNSKPTRVTLDILARYCGYSHWQDFVENQIKASVTSKTNIPELWSLITT
                     NTLEVNHHSIRSIQIKSGIPFEKTVRRTYLESLLNHFVSSNYTALAIVAPGGYGKSTF
                     IAHWYLNNPKVKKQQIIPIIISASKLENYAFSELNIDEWLWGQIGINPFSIPNLKDEL
                     KKLPGSVVFIIDALDEVTLTGTKLERTLKGIHELAKEFSESANTKLIITSRYATWKSL
                     QGYLINPEVWLNIDQSNFNSDNANIPPLSYDEMQTVLDNTVNEGKKERLLIFELPPLL
                     RQTISYPFYLQLFINSYGKATYQTLSDPLNILNEFIKQKVFDSIYPEEKADILNAIAK
                     EWLENSGAITKQQLKKLYPINLKTAGNYFDAYHDLLSFGIISEEKVYSDSLGYSTLVS
                     ISNNNILTSLIAQKLIKEYGFNEKLINATKNLFSLNEIYPKLIGYIFSISFNERKVAF
                     LKDLFQLFLPDYITELTNTLTLCLKRDESLFKDLLPHLLKNRELERELLLISPDLNGT
                     SSTYRKILEQVQPSSPYYKDSKIAMDYCNLIGLEISQINNSNEWINNFILTEQSSARI
                     AGMWFCLNIFFSSSSKSELMVKKALEFYKSINSANAKLEFRESVLPLVFIDKRFFPLQ
                     DIFIEPLNHSAHQKALDSIIYEFTDITKNRTPFTPEKHHQLMQNYSQLNPSRNHFAIV
                     LGELARSISYFYESNLNDAHTCIRNSIELCGISGYRLQEMVLMNILGQTLIRLGETNY
                     GKSFVSYAETLSEKSGVKVYSEHFVCGS"
     gene            4693..5544
                     /locus_tag="PLW22_05260"
     CDS             4693..5544
                     /locus_tag="PLW22_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOU74148.1"
                     /translation="MYSQVWEKCSASVCCLNFYNEHGVLIDTLSGFKINKSLVTCEQA
                     FYVKGAQKVEIRFVEADANTPKASMRLDYKEFIDDLRIGFTHNQADYAVFNIDFAEFQ
                     DIPSLSLCERWNYPIGMPVAIIGFNGVGQNLSIKTGIVSSAFSNKQGVRYLQLDGLTC
                     YGNSGAPVIDPQTMQVIAIVSRRNNPATKSYNDLMGYISTNLDELRKIEGKFKLGELD
                     PIQVLIANQNQIKLLATNIYKHTATGSSQAVMLDHIITYFNEQAIIENHLLKSTEVEF
                     DVTVNKE"
     gene            5713..6045
                     /locus_tag="PLW22_05265"
                     /pseudo
     CDS             5713..6045
                     /locus_tag="PLW22_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            6139..7572
                     /gene="aspA"
                     /locus_tag="PLW22_05270"
     CDS             6139..7572
                     /gene="aspA"
                     /locus_tag="PLW22_05270"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOU74149.1"
                     /translation="MVNPASQYESGATRLEHDLLGEREVPAEYYYGVQTLRALENFNI
                     SGVTLNFYPDLIDALAMVKQAAAEANHELGLLEPTIKDAIIKACTEITNGKFHNQFVV
                     DMIQGGAGTSTNMNANEVIANRALEHLGYSKGEYQYCHPLNHVNMSQSTNDAYPTAVK
                     IALINSNKKLVRVLESLIESFRAKAKEFAHVIKMGRTQLQDAVPMTLGQEFEAYAVTL
                     SEEVERLNQNVRLFLEVNMGATAIGTGINSDPDYPEKVIKHLRKISGLDLVLASNLVE
                     ATQDTGAFVMYSSAVKRLAIKLSKISNDLRLLSSGPRTGLNEINLPPMQPGSSIMPGK
                     VNPVIPEVVNQIAFKVIGNDLTVTLAAEGGQLELNVFEPVIVQSLFESIEMLKNGMNT
                     LKYRCIDGITANEERCRSMVHNSIGLVTALNPYIGYEASTQLAKEALVTNKSVYDLVL
                     QKGLLSKQQLDTILAPENMVKPRKLNL"
     gene            7648..8127
                     /gene="cdd"
                     /locus_tag="PLW22_05275"
     CDS             7648..8127
                     /gene="cdd"
                     /locus_tag="PLW22_05275"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOU74150.1"
                     /translation="MLTIDYTEFHDTNQLKDWEKDLIEQAKAALAGSYSPYSHFRVGA
                     AVLLDNGVIITGSNQENGAYPSGLCAERVALFYAGAQYPNVPIKALAVVASNEKGITV
                     SPVSPCGACRQVMLEYQMISNKPFTIYMVGAGKIIKIDDVKQLLPFSFTNVSEMDQE"
     gene            complement(8132..9736)
                     /locus_tag="PLW22_05280"
     CDS             complement(8132..9736)
                     /locus_tag="PLW22_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU74151.1"
                     /translation="MPYKVLTACGNIQLLEDIQRIFSLADDDFTVSNILDSERIISTI
                     TNNNPDIILYDIHFANKAAADVLSIIKADPATSNIPILAIADFLQPEHIQSLFHAGAD
                     DFIGLPINTPELIQRTMVGIQRGRMLKKLKTLSDQFDSITTAISQAGNSVIIISNTGE
                     ITWVNEGFKRLYECSVEEFKATFGQNLFSETINKTTAEAIRKCIVGEYVVYESKWVTP
                     MGKEKYIQTSLTPVFDDLQNLSYIVAIEMDITDLKLIEKDLAEKNEHLQSIMENLENA
                     NKILEEQQNQIQKQSELLAEEKQKTEALLLNILPLEVANALQKKGIYKPKKFKEVSVM
                     FADFVGFSKISVAYENIDEFLNVLSSYFEAFDEIVTQRYIEKIKTIGDAYMCVGGLPR
                     TNHSHPFDTVLAALELQRYTKARNIEEAQANKPTWSIRIGIHSGSVIAGVIGKHKFAY
                     DIWGDTVNVASRMETACEPGKINISETTYSYIKDYFICTPRGKIPAKNIGEIEMFFVE
                     RIKPEFSEDADGFIPNNALRKIVSSL"
     gene            complement(9837..10046)
                     /gene="xseB"
                     /locus_tag="PLW22_05285"
     CDS             complement(9837..10046)
                     /gene="xseB"
                     /locus_tag="PLW22_05285"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOU74152.1"
                     /translation="MTKKPVKYSEAIEEVERIINQIESNELDIDELTEKIRRASELLK
                     FCKQKLHFTEEEIQKIIDQMQKDDE"
     gene            complement(10027..12912)
                     /locus_tag="PLW22_05290"
     CDS             complement(10027..12912)
                     /locus_tag="PLW22_05290"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74153.1"
                     /translation="MLQKIKVPLFLALLAFNSFSQGSKVNIDSLIATIKKYSIENPEV
                     SAISCNRLFLQVKQNPSDSLRALYHLAMSEYKSGLGLTGDALGHVDSAITITQSKRTQ
                     SLYVDALLKKSALLQRSGNYSEAIKVNEKARLTALKGKNNRLLIQSLLLRGDIYKEIG
                     QYNDALSKKGEALAIAKKIGVGIAECHQSIGSTQWNFGNYQEALKSYFEGLIAAEKDN
                     DTLRIIQLLKNIGLTYRETGNFDKSTSHLSKALQIAEKSKLNDELADILNIMGGVYLK
                     FSQPAKAIEYYKQSLNIRGTLGYKQSSINTLNNLAKAYLLLKNSSESITSLNKALELS
                     KEIGDPLTEANIHNELGNTYLTIEDYPEALRRYLLALKIRQSYGKDEDIARTMVNIAL
                     VYRRLGLFRNAQKYMSEAKGLVENLDAKPDLKIYIYQNMGNVYYDQKQYTNAINAYQK
                     ALELSEKKGDDLQVTRVLRNIAQTYLDLNKPSKARNTITQAIKIALRSNQNQLLADLY
                     NTLGNIERQDNNFDLALTHYNTAKEKYKQNLNADGEALCLRKIGEIYSLQGRYRDAKV
                     NINQSIRIGKSVGNPYLVLLGTLALSDVEKAQGKFQNALTLYQKYVYLKDSIEDAKRS
                     EANIDAHINLELDKTKTEIKLIEAEVEALRTKVELDRERINKQKAQRNLLVTAVIFLL
                     FIAFGSFYLFNQKKNLARLQREKYEEEERINQKLKQYQAELEQNIKTKDKLFSIVAHD
                     LRSPFTALVGLSEILATKADELPPSEVKEFSNHIHQSASNVLALTENLLSWARSQTGK
                     LVLNQAYINICELISKAVEVASLPAGEKEIKIEVICNETVTAFADYDTLLTIIRNLLS
                     NAIKFTPGGGKISLKASSDNQKVTIQVTDTGVGIAPENLSNLFRLDGFTTKGTNSESG
                     TGLGLMLCKEFAEANGGTISVSSTLGKGTTFTITLPAKNVMYDQETC"
     gene            complement(12984..15176)
                     /locus_tag="PLW22_05295"
     CDS             complement(12984..15176)
                     /locus_tag="PLW22_05295"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HOU74154.1"
                     /translation="MRSNNIKILLNLIVIFYLPIAGFTQQPSSSIDSLRLKLNNAKGI
                     EKVNILIDISKYYWDVNPDSSVIFAEKSLKEAKADGSDDAIGDAYNALGNAYSSKYQN
                     ETALNYYLKSLEFRKLNGNPLKIGNSYHNLGLVYRELKQFNLAVSSHTKAADYYEKAK
                     RYDLQSISYNRIASLYNQINDKNKALEYAFKALNIVNENNIDELKANLYNLIGSLNRD
                     LKNLELAEKYYTLAFKEWEKSKNISGLSTAYNNLGIVLDEKGDLQKALEYYTKSLEFA
                     TQQRDSSGIATAYNNIGFLYARIKQFDKALMSYQKSYEISVKTNGVDAAHNTLNNIAS
                     TYLKQNNLEKASEALNKALRNIPNVVDQAYKQETYQLLSDLYAKKGDYRNAYHYKCVE
                     LAYADTLYTQQRLSSIAEMQARYETETKEKEIELLKKDVELKELQFQKQRSVQQVLIS
                     LTLLLLAIFFGTSLILTIIRKKNKQLAEKNAELFEINKKLKESEKNLSELNATKDKLF
                     SIIAHDLKNPFNALIGFSDILERNFNHLTDNEKKEYISVISESAQNLYKLLDNLLQWT
                     RAQTGSINYIPEKFKLEPLIKQEVLNLNANAEKKRINVSVNASSAISVYADKNSIATV
                     IRNLLSNAIKFTDIGGTIEIIASESKDFPKMAEVVVKDSGIGIDQDDLERIFMIDGSY
                     STKGTANETGTGLGLLLCHEFVTKNNGKIWVNSSKGSGSEFHFTIPLA"
     gene            complement(15207..16580)
                     /gene="xseA"
                     /locus_tag="PLW22_05300"
     CDS             complement(15207..16580)
                     /gene="xseA"
                     /locus_tag="PLW22_05300"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOU74155.1"
                     /translation="MVEKQKTYSLKELNLLVKETLSQYLSQAYWIVGEISEIKEHYSG
                     HCYLELIQKDEESDNIVARARATIWSYSYRMLKPYFESTTGRKLSPGLKVMVLAQVSF
                     HEVYGYSLNIIDIEPTFTIGDLELKRRETINRLIADGVFDMNREQTLDLLPNRVAIIS
                     SPQAAGYEDFKKQLLGNKARYRFSIKLFEAVMQGEDAENSIIQALSNIYNEIDRFDLV
                     AIVRGGGASADLSCFDSYSIASHIAQFPLPVITGIGHEKDTTVADLVAFKAVKTPTAL
                     AEYLIDLASEAEQLYLTLLDSLVELSTSYTESSKSQLIVYADQLKSASQLTVFHHKNR
                     LTKTWSSLPVLAKFYMFKSLSQVQGLKEQVKSAAENYVNTPKIAIEKYQKDLKKYTLQ
                     TLSANKQKVDFFGRMIETMNPINTLKKGFTITMIDGKTIKSASEIGEGSLLTTKFWDG
                     EVKSVVK"
     gene            complement(16580..18217)
                     /locus_tag="PLW22_05305"
     CDS             complement(16580..18217)
                     /locus_tag="PLW22_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU74156.1"
                     /translation="MKNYQNHLRIILLLFIISGLVPLRAFAQAKYFIAFTDKQNSTYS
                     VDNPNEFLSARAIERRAKQGIAISTDDLPVTPAYVSQVAQSATMVHYSLRWFNGVVAT
                     LTPEQLTSVQGLPFVKNITKIYEPAKSETDDKTEWQPLGTNEKREFAGLNYGASYTQI
                     AMMNGHLLHANGYLGQEKVIAVLDAGFLNVNTHNAFDSLWQRGRILGARDFVNPQSDI
                     YKEHYHGTMVLSAMGGYIDGQLIGTAPRANYWLIRTEDAATEQIIEEYNWAAGAEFAD
                     SVGADIINSSLGYTTFDVASQNHTYQDLNGNTAPVTLAANMAASKGMVVVVSAGNDGN
                     APWHYISVPADSPNVLTVGAVDNKRVKADFSSFGPTADGRIKPDVCAMGKGTVLAIAT
                     GTTGTSNGTSFSSPLMAGMVACLWQAKPSLTAQQIVELVKSSSSNNASPNNSTGYGIP
                     DFAQASTSSITDARNSIELWPNPFTSKINILLGKSFIPANISIFTTNGQLILTMNAFS
                     DGTGHVEINISEELPKGIYLLSIDISGSRYYVTGIKQ"
     gene            complement(18232..19032)
                     /locus_tag="PLW22_05310"
     CDS             complement(18232..19032)
                     /locus_tag="PLW22_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOU74157.1"
                     /translation="MKRILAFLVLLIFSGLAIGQTDGAKNQLDSKNRKQGYWCKYYPN
                     GKKAYEGWFKDDKPVGEFKRYHQNGELKVIMVYSESSPYVITTFFNEDGKKIVSGFYK
                     NQQKDSLWQYYSMEGKLIFEERYKNGLKNGKTRSFYPSGKVLESASYTNGNLNGLVVQ
                     FFENGLNKSIIPYTNGEIDGTMKVFNPSGTTRIEGSYIKGLKHGEWKFYDTDGKITQT
                     IKYFNGVAENENELIEKESKYIESLLKNAGKLKEPSIDDVMNSMGTRY"
     gene            complement(19223..19828)
                     /locus_tag="PLW22_05315"
     CDS             complement(19223..19828)
                     /locus_tag="PLW22_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOU74158.1"
                     /translation="MSNSYKYIDPDYSYIDPKTGILRNLQDISDPEVLLFVESSAVTK
                     RLLELYENPIKIKGIDSLFEIHRHLFQDIYIWAGKKRVVEISKDGKQFFPTTYFDNAF
                     RYIDQLIDEYRKIPKNNKRKLAEKLAEILDNVNYLHPFREGNGRAQREFIRLLALEKG
                     LILNLNPPDNISVYERYMKGTVESDVTTLTELIFELITADK"
     gene            complement(19821..20042)
                     /locus_tag="PLW22_05320"
     CDS             complement(19821..20042)
                     /locus_tag="PLW22_05320"
                     /inference="COORDINATES: protein motif:HMM:NF037437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HOU74159.1"
                     /translation="MFNAIEVDRKNLTIMGVKFSDLKTLELTARALGSNMFEGFTPTQ
                     KGIEIIRDYVTGKISLTELVAFAKQKAYV"
     gene            complement(20186..>21783)
                     /gene="dnaK"
                     /locus_tag="PLW22_05325"
     CDS             complement(20186..>21783)
                     /gene="dnaK"
                     /locus_tag="PLW22_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOU74160.1"
                     /translation="RLYTPQEISSMILQKMKKTAEDYLGHEVTEAVITVPAYFSDSQR
                     QATKEAGEIAGLKVRRIINEPTAAALAYGLDKKNKDIKVAVYDLGGGTFDISILELGD
                     GVFEVKSTNGDTHLGGDDFDQVIIDWLAEEFLKDEGVDLRKDPMALQRLKEAAEKAKI
                     ELSSTTTTEINLPYIMPVNGIPKHLVKTLTRAQFEKLADHLIQRTVEPCRKALADAGL
                     QPSDIDEVILVGGSTRIPKVQEVVEKFFNRKPNKSVNPDEVVAIGAAIQGGVLTGEVK
                     DVLLLDVTPLSLGIETLGGVFTKLIEANTTIPTRKSEVFTTAADNQPSVEIHVLQGER
                     PLAKDNKTIGRFHLDGIPPAPRGVPQIEVTFDIDANGILHVTAKDRGTGKEQKIRIEA
                     SSGLTEEEIKRMREEAKRNEEADRREKERIEKINAADSLIFQTEKQLKEFGDKLPADK
                     KASIEAALGKLKDAHKSQNLAAIENATNELNAAWQAASEELYKAQAQANAGAGQQANA
                     NAGSSSSSGNDQEVTDVDFEEVK"
BASE COUNT         5769 a         4362 c         4605 g         7047 t
ORIGIN      
        1 gccctactgc tatgttgtcc ctcacgaagc ggtttcgttt tatggtaagg attttggcca
       61 caacccggtt ggcaccggtc ccttttacct gaagctttgg cgcgatgggg aaaagcttgt
      121 gttacgcaaa aacccaaatt actttgaggt tgatgagcta ggtaaccgcc taccttacct
      181 cgaggctgta tccatttcat ttattcccga taagcaatcg gaatttctgg aatttgtaaa
      241 tggtaaaatc gactttcttt caggggtaaa cgcagcatca aaggatgaac ttcttacccg
      301 ttcgggtaag ctaaatccca agtattccgc taaggttaaa atgattacag gcccatacct
      361 aaatactgaa tacattggga tacttgttga ttctacaaaa cttcccagta accaacgatt
      421 tttgctcgac gagcgggtac gtaaagctat tgctttggct tttgataagg agaaaatgat
      481 gctttacctt cggagcaacc ttggctatcc ggcttatgga ggatttgttc cttccggcat
      541 gcctggtttt actgatgtaa actacggttt ggcttacaac ccattacagg caatgcaatt
      601 gctagagcaa gcaggttaca gcagtaaaaa cgctttacct gaaataacag tttcaaccac
      661 cgacgattat ctcgatttgt ttgagtacat tcaaaaccag ctcagtttgg taggcattaa
      721 actcaatatt gatgtgctac ccggtgcagc ataccgcgaa atgcttgcaa atgggaaact
      781 acccgttttc agagggtctt ggatagctga ctatcccgat ccggaaaact acctagcttg
      841 tttccttact gataattttg ctcctaatgg accaaactat agccatttca gcagtgtgca
      901 gtttgaaatg ctttatcgga aagcaatgtc acttaccagt tacgaccaac gattaccata
      961 ctaccatagc ctcgattcta ttgttacatc aaaagccata gttatacctt tgtactacga
     1021 taaagtggtt aggtttacct cggccagagt tgatggtatt ggtgtaaatc cgatgaattt
     1081 tttagttttg aaaaaagtaa aagtaaaaag cataaaatga taattattgc cgatagcgga
     1141 gctacaaaaa ccgattggag aatcatagaa cacggcaagc ccattgcaag tttcgaaacc
     1201 attgggttaa gtccatactt taacaggcag gaagattttt taagcgcatt aaatcaaaac
     1261 taccccaagc agatcaaccc agaacaggta aaggagattt tcttttacgg atctggatgc
     1321 ggattgcatg aaagggggca ggatgtagca aggtatcttg gtattttttt tagcaacgcc
     1381 aaaatccatg cagttaccga tgctttaggg gcagcccggg cgctatttaa gcgccaaacc
     1441 ggtattgttg taatccttgg aactggctca aatattgcct actacgatgg cgaacggctt
     1501 aataaccgaa ccccctcgct tggatttgtt ctgggtgacg agggtagtgg agcttacatg
     1561 ggacggctgt tgcttcgtgc atatcattac aaaacacttg atgttggttt agcccaaaaa
     1621 cttgagcaac agtacaatgt tgagctaagc catgtgctca atatggttta tagtgcaccc
     1681 cgcccatcgg cataccttgc atcgtttgta ccttttattc ttgagaatat acataaccag
     1741 catatatact ctattgtaaa ggaatcgttt gtagcccttt acaggtacca ctttgcggtt
     1801 tttgagaatc tatccgactt atcaattggt gtaaccggtt ccgtaggatg tctttttagt
     1861 caggttcttg attcggttgg tgaggagtat gggtttaaaa tatcgcaata cttgcgctac
     1921 ccaatagttg agcttattaa ctaccacaca caaaatgctc agaatacacc ttaacacccg
     1981 atttttcaga aagagtttcg gcatatgaaa caaacgattt tccatagttt gtttcgccta
     2041 gtcgtattag ggtttgacca agtatgttca tcaataccat ttcctgtaag cggtaacccg
     2101 atattccaca aagttcaata gagttcctta tacaggtatg tgcatcgttc aggttgcttt
     2161 cgtaaaagta cgatattgag cgtgctagtt ctccgagtac tattgcaaaa tggttcctac
     2221 tgggattgag ctggctgtag ttttgcataa gttgatgatg cttttccgga gtaaatgggg
     2281 tacgattttt agtgatatcg gtaaactcat aaatgataga gtccaacgct ttttggtgtg
     2341 ccgagtgatt taagggctct atgaaaatat cttgaagtgg gaaaaagcgc ttatcaatga
     2401 aaactaacgg taaaaccgac tccctaaatt caagcttagc attggcggaa ttgatacttt
     2461 tatagaattc taaagctttc tttaccatta gttcggattt tgaactagat gaaaagaaaa
     2521 tattaaggca gaaccacata cctgcaattc tggctgaact ttgctcagtc agaataaaat
     2581 tattaatcca ctcattgcta ttgttaattt gcgagatttc caaacctata aggttgcagt
     2641 aatccattgc tatttttgaa tccttgtagt aagggcttga gggttgcact tgttctagta
     2701 ttttcctata ggttgatgag gtgccattca aatcgggact tatgagcaat aactccctct
     2761 ctaattctct atttttgaga aggtgaggaa gcaaatcctt gaatagtgat tcatcgcgtt
     2821 ttaaacatag cgtgagggta ttggtgagct ccgtgatgta atcgggcaaa aatagttgga
     2881 acaaatcctt aagaaaagca accttacgtt cattaaagct aatgctaaat atgtatccaa
     2941 tcaattttgg gtaaatctcg tttagtgaaa agagattttt tgttgcattg attaatttct
     3001 cgttaaagcc gtactcctta attagttttt gtgcaataag cgatgttaat atattattat
     3061 tgcttatgga aacaagagtt gagtagccta gtgaatcgga atacaccttc tcttcagaaa
     3121 taattccgaa cgaaagtaaa tcatggtaag cgtcaaagta gttgcctgca gtttttaggt
     3181 ttatggggta gagcttttta agctgctgct tggtgattgc accagagttt tccaaccatt
     3241 ctttggcaat ggcattcaaa atatcggctt tctcttcagg gtaaattgaa tcaaatactt
     3301 tttgtttgat aaactcgttg aggatattca gtgggtcgga aagggtttgg taggttgcct
     3361 ttccgtagct gttaatgaaa agttgtaggt aaaatgggta cgaaatggtt tggcgtagca
     3421 agggcggtaa ctcgaatatg agcaagcgtt cctttttccc ttcgttcaca gtgttatcaa
     3481 gaactgtttg catttcatcg tacgaaaggg gagggatatt ggcgttatcg gagttgaagt
     3541 tggattgatc aatgttcagc caaacctctg ggtttataag ataaccctgc agcgatttcc
     3601 aagtggcata ccttgatgta ataatcagct tggtgttggc cgattctgaa aactctttgg
     3661 ccagttcgtg tattccttta agtgttcttt caagtttagt gcctgtaagg gtaacctcat
     3721 caagggcatc aattatgaat acaaccgagc caggcagttt tttgagctca tcttttaaat
     3781 ttgggataga aaatgggtta atgccaattt gaccccacaa ccattcatcg atatttagct
     3841 ctgaaaacgc atagttttca agttttgatg ccgaaatgat gatgggtatt atttgctgtt
     3901 tttttacttt agggttattt aagtaccagt gggcaatgaa agtagatttt ccataaccgc
     3961 ctggcgccac aattgccaat gctgtgtagt ttgaacttac aaagtgatta agcaacgatt
     4021 ccagatatgt tcgccttacg gttttttcaa atgggattcc ggattttatt tgtattgaac
     4081 gaatgctatg gtggtttacc tccagtgtat ttgtggtaat taaactccaa agctccggta
     4141 tgtttgtttt agaggtaaca ctcgccttaa tctgattttc aacaaagtct tgccagtggc
     4201 tatacccgca atagcgtgca agaatatcga gtgtgacacg tgttggttta gaattcgttt
     4261 tcaggaaccc aaaaagccgg cggagggttg ccgggcttat atactcgtgt gtatgctcaa
     4321 atactttttc cgacaaggtt ttacagtcgc gttggtacga tatcttttta ccaaaacgct
     4381 tctcaacggc ttcaataagc tgtttatgta aatccattgc cgaactgctt ttgatgtctc
     4441 aaatgtaagg attttaaaga tatctataaa tcgggttgta aagggttttc ttaaaaattt
     4501 attgacacgt ttgacacgtt actctccgag gataactacc gtatatttgg caaggagcaa
     4561 aacaataatg ctatgttcaa gcttgtaaaa aagaataaaa ttgacattgg ggtgttcatc
     4621 atttttttca tcatcaccct aatttttgta gtaacgctag taaaaacttt aacattataa
     4681 atttaaaaaa ccatgtacag tcaggtttgg gaaaaatgtt cagcatcggt ttgttgtctg
     4741 aacttttaca atgagcacgg tgtgctcatt gacacgttga gtggatttaa gatcaataaa
     4801 tcgcttgtaa cctgtgagca agccttttac gtaaaaggcg cccaaaaggt agaaatccgt
     4861 tttgttgagg ctgacgcaaa taccccaaag gcatccatgc gtttggatta caaagagttc
     4921 attgatgacc ttaggattgg gtttacccat aaccaagccg attacgctgt atttaatatt
     4981 gattttgctg aatttcagga tattccaagc ctatcgctgt gcgaacgatg gaattaccca
     5041 attggcatgc ctgtggcaat tattggtttt aatggagtag ggcaaaacct ttccataaaa
     5101 acaggaatag tttcgtccgc tttctccaat aagcagggag ttaggtacct acagctcgat
     5161 ggcctaactt gctatgggaa tagcggagct cctgtaattg accctcaaac catgcaggta
     5221 attgcaatag tttcgcgtag aaataacccg gcaaccaaat cgtacaacga tttaatgggc
     5281 tacatcagca caaaccttga tgagctgcgc aagattgaag gtaaatttaa acttggtgaa
     5341 ctcgacccca ttcaggtact tattgccaac cagaaccaga ttaagctttt ggccacaaat
     5401 atctacaagc acactgcaac aggttcatcg caggcagtta tgctcgatca tattatcact
     5461 tactttaacg aacaagcaat tattgaaaat catttattaa aaagtaccga agtggaattt
     5521 gatgtaacag ttaataaaga gtagtgtttt tgttttcatg ggttggtagg gtactgctat
     5581 catgcagtgc cctttctttt ttatgttaaa caattataaa aagtaaaatg tttgtaattt
     5641 gcatgaacaa taagaatcaa gcatacttag ttttgtgtta gttttttatg gtgaaactta
     5701 aatgataagg aaatgagtcg aaagagttat gttgattttc tgagtaatat agaacttttc
     5761 aggggattga gtgagcagga actgaataca cttgtcaaag ggattgttga aagggaattc
     5821 cctaccggaa gcatgttgtt cattgagaat aataaacgcg agggcattct ggtgatatat
     5881 gaggggcagg ttgaactttt taagagcaat ccttatggag ttgagcttaa gctaacccaa
     5941 ttctcaaagg gcgatttcct tggcgaaggg gttctagcct cagaatctac tcattctaca
     6001 tcggcaaggg ctatagctcc aacaaaggct ttactcctga gggttgagga tttgaatgca
     6061 aatcctgcaa ttgcactcaa ggtacttacc aatattaccc gggttatgca gcggcgaatg
     6121 aggtatgcca acaaccgtat ggtgaaccct gctagccagt acgaatcagg tgcgacccgc
     6181 cttgagcacg acttgcttgg agagagggaa gtgcctgccg agtactacta tggagtgcaa
     6241 accctaaggg cattggagaa ttttaacatc tccggggtaa ccctgaactt ttaccccgat
     6301 ttaattgatg ctcttgcaat ggtgaaacag gctgctgccg aggcaaacca tgaactaggt
     6361 ttacttgaac ccaccattaa agacgccata attaaagctt gcactgaaat aaccaatggg
     6421 aagttccaca atcagtttgt ggttgatatg attcagggtg gggcgggtac ctccacaaat
     6481 atgaatgcca acgaggttat tgctaaccgc gcactggaac atttggggta ttcaaagggg
     6541 gagtatcagt actgccatcc actgaaccat gtgaatatgt cgcagtcaac caacgatgct
     6601 taccctactg ctgttaaaat tgcgcttatc aactcaaaca aaaaactggt aagagttctt
     6661 gaatccctga ttgaatcgtt tagagcaaag gcaaaagagt ttgcccatgt aataaaaatg
     6721 ggaagaacac agcttcagga tgctgttccc atgactcttg gtcaggagtt tgaggcttat
     6781 gcggttaccc tttccgaaga ggtggagcgc ttgaatcaga atgtacggct attcctagag
     6841 gttaacatgg gggctaccgc aattggaacg ggtataaact cggaccccga ttacccggag
     6901 aaagtaataa agcatttacg taaaatatcc ggcttagatc tggtacttgc ctccaacttg
     6961 gttgaggcaa cccaggatac cggtgcattt gtaatgtact catcggcagt aaagaggcta
     7021 gcaattaagc tttccaagat atccaacgat ttacgcctgc tgtcctccgg cccgcgaacg
     7081 ggtttaaatg aaattaattt accgcccatg cagcccggct catcaattat gcctggcaaa
     7141 gtgaatcctg taattcctga ggtggtaaac caaattgctt tcaaggtaat tggaaacgac
     7201 ttaaccgtaa cccttgccgc cgagggaggt cagcttgaac ttaatgtttt tgaacccgtt
     7261 attgttcaga gcctttttga atcaattgag atgcttaaga atggcatgaa tacgttaaaa
     7321 tatcgttgta ttgatggaat tactgcaaac gaggagcgct gtaggagtat ggtgcacaac
     7381 tcaattggtc tggtaacagc cctaaatcct tacattggtt atgaagcatc aacccagctt
     7441 gccaaggagg ctttggtaac caataaaagt gtgtatgacc ttgttttgca aaagggttta
     7501 ctatccaaac agcagctcga taccattctt gcccctgaaa atatggttaa accaagaaaa
     7561 ttaaatctgt aagtacgata ttatctaaaa atccttacat ttgatgcact cccttttttg
     7621 ttgggagtgc attaaaaata tgtggcaatg ctaacaattg attatactga gtttcacgac
     7681 accaatcaat taaaggattg ggagaaagat ttgatagaac aagctaaagc agctttggct
     7741 ggcagctatt caccttactc ccattttagg gttggagcag cagttttact ggacaacggg
     7801 gtaataatta ccgggagtaa tcaggaaaat ggtgcatacc cctcaggtct gtgcgcagag
     7861 cgagtagccc ttttctacgc gggtgcgcaa taccctaacg ttccaattaa agcattagct
     7921 gttgttgcat cgaacgagaa agggattacc gtaagtccgg tatcgccatg cggtgcgtgt
     7981 aggcaagtaa tgctggaata tcagatgata tccaataaac cctttaccat ttatatggta
     8041 ggagcgggaa agattatcaa aattgatgat gtaaagcaac tacttccctt tagctttacc
     8101 aatgtaagcg aaatggatca ggagtaaagg tttatagcga ggaaactatc tttcgtaaag
     8161 cattattggg gataaaacca tcggcatcct cggagaattc cggttttatt ctttccacaa
     8221 agaacatctc aatttcaccg atattcttgg cgggaatttt acctcgaggc gtacaaataa
     8281 agtaatcctt gatgtacgag tatgtagtct ctgaaatatt aattttccct ggttcgcatg
     8341 ctgtttccat tcggctagcc acattaaccg tatcgcccca gatgtcgtaa gcaaatttgt
     8401 gtttaccaat aaccccagct atcacactgc cagagtgaat accaatacgt attgaccagg
     8461 taggcttatt ggcctgcgcc tcctcaatgt tccttgcttt agtatagcgc tggagttcaa
     8521 gtgctgcaag cacagtgtcg aacgggtggc tatggttggt tcttggcaat cctcctacgc
     8581 acatgtatgc atcgcctatc gttttaatct tctcaatata gcgctgggta actatttcgt
     8641 cgaatgcttc aaagtagctg ctcaggacat taaggaactc atcaatattc tcgtatgcca
     8701 cacttatttt tgagaatcca acaaagtcgg caaacataac cgaaacttcc ttgaactttt
     8761 taggcttata tatccctttt ttctggaggg cgttggcaac ctcaagtggt aaaatattta
     8821 gcagcaaggc ctcggttttt tgtttttcct ctgcaagcaa ttcggactgt ttctgaattt
     8881 ggttttgttg ttcctctagg attttgttgg cattctcaag gttctccatt atgctttgga
     8941 gatgttcatt cttttcggct aggtctttct ctattaactt aaggtcggta atatccattt
     9001 caatggctac aatgtagctc aggttctgca agtcatcaaa aacaggtgtt agactggttt
     9061 gaatatattt ctccttgccc atgggagtga cccatttact ttcgtaaacc acatattccc
     9121 caactataca ttttctaata gcttcagcag ttgttttatt tatggtttcg gagaaaaggt
     9181 tttggccaaa ggttgcttta aactcctcaa cactacattc gtaaaggcgt ttaaagccct
     9241 catttaccca tgtaatttcg cctgtattgc taataataat tactgagtta cccgcctggc
     9301 tgatggcagt tgtgatgctg tcgaactgat ccgatagggt tttgagtttt ttcagcatac
     9361 gtccgcgctg aatccctacc attgttcgct ggataagttc aggggtgttt attggcaagc
     9421 caataaagtc gtcggcacca gcgtggaata gcgattgtat atgttccggt tgtaagaaat
     9481 cggcaatggc cagtatgggg atgttagatg tagccgggtc ggcctttatg attgatagca
     9541 catcggcggc agccttgttg gcaaagtgga tatcgtaaag gataatgtcc gggttgttgt
     9601 tggtgatagt tgaaataata cgctcggaat caagaatatt acttaccgta aaatcatcgt
     9661 cggcaaggct gaatattcgt tgaatatctt ctaaaagttg aatgtttcca caagctgtta
     9721 aaactttgta aggcattttt caattccgat cttaaagcaa aaaaacgaaa aattatgtat
     9781 taatggaaac gtttgcggaa attatttttc gcccgcatcc aagtaaaaag cattttttac
     9841 tcatcatctt tctgcatctg gtcaataatt ttttgaattt cctcctcggt aaagtgaagt
     9901 ttttgtttgc agaattttag tagttccgag gcgcgtcgga ttttctccgt taactcatca
     9961 atatcaagtt cgttcgattc tatttggttg ataattctct caacctcctc aatagcctcg
    10021 ctatacttaa caggtttctt ggtcatacat tacatttttt gcgggtaaag ttattgtaaa
    10081 tgttgttccc tttcccaggg tactacttac gcttatggtg ccgccattag cctcggcaaa
    10141 ttccttacaa agcattaatc ctagtcctgt tccgctctcg gaattggttc ccttggtagt
    10201 aaaaccatcg agccgaaata ggttactcaa attttcaggg gctattccaa cacccgtatc
    10261 ggttacttga attgtcactt tttggttatc ggacgaagcc tttaatgaaa ttttgcctcc
    10321 acctggggta aactttatgg cattgctaag cagattccgg attatggtta gtagagtgtc
    10381 gtagtcggca aaagcggtca ctgtttcatt gcatattacc tcgattttta tttctttttc
    10441 acctgcaggc aacgatgcca cttcgacagc tttgctaatt agttcgcaaa tatttatgta
    10501 tgcttgattt aacactaact tgccagtttg actccgtgcc cagctaagaa ggttctcagt
    10561 gagtgcaagt acatttgagg ctgactggtg tatgtggttg ctaaactctt tcacctcgga
    10621 gggcgggagt tcatcggctt tagtggccaa aatctccgaa agacctacaa gagcggtgaa
    10681 ggggcttcgt aaatcatgag ctacaatgga aaacagttta tccttggttt taatgttttg
    10741 ttcaagctcc gcctggtatt gctttaattt ttggtttatc ctttcctcct cctcatattt
    10801 ctcccgttgg agtcgggcta aattcttttt ttggttgaaa aggtagaaac tcccaaatgc
    10861 aataaaaagc agaaaaatta ctgctgttac caggagattg cgctgtgctt tttgcttgtt
    10921 tatgcgttcc ctatcgagtt caaccttggt tcgcaatgct tctacctcgg cttcaatcag
    10981 ttttatttca gtcttggttt tatccagttc caggttgata tgtgcatcaa tgtttgcctc
    11041 gctgcgtttg gcatcttcaa tgctatcctt aaggtaaaca tatttttggt aaagggttaa
    11101 ggcgttctgg aacttaccct gtgccttttc aacatcgctt agtgctaggg ttccgagcaa
    11161 aacaaggtat gggttgccaa cgctttttcc aatacgtatt gattggttta tatttacttt
    11221 tgcgtccctg tatcttcctt ggagtgagta aatctcacct atttttctaa ggcaaagcgc
    11281 ttcgccatcg gcatttaagt tttgcttata tttttccttt gcggtgttat aatgggttag
    11341 cgccaggtca aagttattat cctggcgttc aatgttgcca agggtgttat ataagtccgc
    11401 aagtaactga ttttggttac ttctcagtgc aatttttatt gcttgggtta tggtatttct
    11461 ggctttactt ggtttattta aatcgaggta ggtttgggca atgtttctta gcacccgggt
    11521 aacctgtagg tcatcacctt tcttctcgct cagttctaat gctttctggt aagcgttaat
    11581 ggcattggta tattgtttct ggtcatagta tacattaccc atattttggt atatgtatat
    11641 ctttagatcc ggcttagcgt caaggttttc aactaagcct tttgcttcac tcatatactt
    11701 ttgggcattg cggaataagc ctaggcgccg ataaaccagg gcaatgttta ccatggtgcg
    11761 agcaatatcc tcatctttcc catacgactg gcggattttt agagccaaca ggtacctgcg
    11821 gagcgcctca gggtaatcct caatggttaa ataggtattc cctagctcat tgtggatatt
    11881 ggcttcagtg agcgggtctc caatttcttt tgatagttcc agtgccttgt tgagcgatgt
    11941 tatagattcg ctgctatttt ttagcagcaa gtaggctttg gccaaattgt tcagggtgtt
    12001 aatgctcgac tgtttatagc caagtgttcc cctgatgttt aggctttgtt tgtagtattc
    12061 aatggccttt gcaggctgag aaaacttaag gtaaactccg cccataatat ttaaaatatc
    12121 ggccagctca tcgtttagct tgcttttttc tgctatttga agggctttgc taagatgact
    12181 tgtcgatttg tcgaaattgc ctgtttcgcg gtaggttagt ccgatattct ttaaaagttg
    12241 aataattcta agggtgtcgt tatccttttc agctgcaata agcccttcaa agtacgattt
    12301 aagtgcctcc tggtaattac caaagttcca ttgagtgctg ccaatgcttt gatggcattc
    12361 agcaatgcct acaccaattt tttttgcaat ggctaatgct tcaccctttt tgctcaaggc
    12421 gtcgttatac tggccaattt ccttgtaaat atctccccgt agtagcaagc tttgaataag
    12481 taagcggtta tttttccctt tcaaagctgt aagccttgcc ttttcattaa ccttgatagc
    12541 ttcgctgtaa ttacccgatc gttgcagtaa tgctgatttt tttaaaagag catcaacgta
    12601 taaggattgg gttcttttgg attgtgtgat ggtaattgct gaatcaacat ggcctaaggc
    12661 atcgccagtt aagcccagtc cactcttgta ttcgctcatg gcaaggtggt atagcgctct
    12721 aagtgaatcg ctggggtttt gtttcacctg taagaaaagt cgattgcaac ttatggctga
    12781 aacttccgga ttctcaatgg agtacttttt aatggtggca attaagctgt caatattgac
    12841 ctttgaaccc tgtgaaaagg agttaaaggc caacagggct aaaaaaaggg gaactttaat
    12901 tttttgcaac attttaatta ctataagtga tgctcatagc aaagtaaggg aaaaaaatga
    12961 ttatgacaaa caaatgggat gggttatgca agtggtatag taaagtgaaa ctcgctacca
    13021 ctccctttag agctgtttac ccaaatttta ccattgttct ttgttacaaa ctcgtggcaa
    13081 agcagtagcc ccagtccggt acccgtttca ttggctgtgc cctttgtgct gtaggaaccg
    13141 tcaatcatga agattctttc aaggtcatcc tgatctatgc ctatacctga atccttaaca
    13201 accacttctg ccattttagg aaaatctttc gattcacttg caattatctc gatagttcct
    13261 ccaatatcag taaatttgat tgcattactc aataggtttc ggattacggt tgctatgcta
    13321 tttttatcag cgtaaacact aattgctgaa cttgcgttga cggaaacatt tatccttttt
    13381 ttctcggcat ttgcgttaag gtttaacact tcctgcttta tcaaaggctc aagtttgaat
    13441 ttctcgggaa tgtagttaat gctaccggtt tgggctcgtg tccactgtaa aaggttatcg
    13501 agcagcttgt aaagattctg agccgactca gagattaccg agatgtattc tttcttttcg
    13561 ttatcggtca ggtggttaaa gttgcgttca agaatatcgc taaacccgat taaggcgtta
    13621 aatggatttt ttaaatcgtg ggcaataatg gagaatagtt tatccttggt ggcattcagc
    13681 tcgctaaggt ttttctctga ttctttcagc tttttgttta tttcaaataa ttcagcgttt
    13741 ttctcagcca gttgcttatt ctttttccga attatggtta aaattaatga tgtgccaaag
    13801 aatatagcca gcaataaaag agttagggaa attaatactt gctgtacgct tcgttgtttt
    13861 tggaactgta actcctttag ctcaacatct ttcttcagga gttctatttc tttttctttg
    13921 gtttcggttt cgtaccgtgc ctgcatttcg gcaatgctgg aaaggcgttg ctgggtatag
    13981 agagtatcgg cgtatgcaag ttcaacacac ttgtaatggt aagcatttct ataatcgcct
    14041 ttttttgcat agaggtcgct cagtagctgg taggtttcct gtttgtaggc ttgatctaca
    14101 acattgggaa tgtttcggag cgctttatta agcgcttcac ttgccttttc aagattattt
    14161 tgcttgaggt atgttgatgc aatattgttt agtgtattat gggcggcgtc aactccattt
    14221 gttttcactg aaatttcata agatttttgg tagctcatca aggctttgtc aaattgttta
    14281 attctggcgt ataggaaacc aatgttgttg taagcggttg ctatgcccga tgaatccctt
    14341 tgttgggtag caaattccag cgattttgta taatactcca aagccttctg caaatcgccc
    14401 ttttcgtcga gtactattcc taagttgttg taggcagtgc taagtccact aatgtttttg
    14461 cttttttccc actccttaaa agcaagggta tagtacttct cagccagttc caaatttttc
    14521 aaatccctat tcagcgatcc tatcagattg tacaggttag cctttaattc gtcaatattg
    14581 ttctcgttta caatatttaa tgctttaaag gcgtattcta acgctttatt tttatcgttt
    14641 atttggttgt aaaggcttgc aatacggtta tatgagattg attgtaaatc gtatcgtttg
    14701 gctttttcgt aatagtctgc tgccttagta tgactgctaa ctgctagatt gaattgctta
    14761 agctctctgt aaaccagccc aagattatga tatgaatttc caatttttaa agggttgcca
    14821 tttagtttcc tgaactccaa cgatttcagg tagtaattta aggctgtttc gttttggtac
    14881 tttgaggagt aggcattccc cagggcgtta taggcatcgc ctatagcatc gtcgctgcca
    14941 tcggcttttg cctctttgag cgatttttca gcaaaaatga cggacgagtc agggtttaca
    15001 tcccagtagt attttgatat atcaataaga atgttgactt tctctatgcc cttggcatta
    15061 ttaagcttta accttaagct atcaattgaa cttgatggtt gctgggtaaa gccggcaata
    15121 ggcaggtaaa atataactat taggtttaat aatattttaa tattgttact tctcacacct
    15181 tttttttatc aaattacgaa taaaatctat ttcacaacac ttttaacttc gccatcccaa
    15241 aatttagttg ttaacaagct accttcccca atttcactag cgcttttaat ggttttcccg
    15301 tcaatcatgg ttattgtaaa ccctttcttt aacgtattta ttgggttcat tgtttctatc
    15361 attctgccaa aaaaatctac tttttgtttg tttgccgata gggtttgaag cgtatacttt
    15421 tttaagtcct tttgatattt ttctatggcg atttttggag tattaacata gttttcggct
    15481 gcagacttta cctgttcttt caaaccttgt acttgtgaca agctcttaaa catgtaaaac
    15541 tttgcaagta ctggtagcga tgaccaggtt tttgtcagcc tgtttttatg gtgaaacacc
    15601 gtaagctgac ttgccgattt gagctggtcg gcataaacaa tcagttgcga ttttgaactt
    15661 tcggtgtagc ttgttgatag ttccactagg ctgtcaagta gtgtaaggta aagctgctca
    15721 gcctcgctag ctaaatcgat aagatactcc gcaagggcag taggtgtttt tactgctttg
    15781 aaagcaacca aatcggcaac tgttgtgtcc ttttcatggc caattccggt gataacgggc
    15841 agtggaaact gtgcaatatg gcttgcaata ctgtatgaat caaagcacga taggtcggca
    15901 gaggcgccac ccccacgaac aattgccacc aggtcaaacc tatctatctc attgtagata
    15961 tttgaaagtg cttgaattat ggagttttca gcgtcttcgc cttgcataac ggcttcaaaa
    16021 agcttgatgc tgaaacggta tcgggctttg ttgcctagca gttgcttctt gaagtcttcg
    16081 tatccagcag cctgcggcga cgatattata gcaactctgt tagggagtaa atccagagtt
    16141 tgttcacgat tcatgtcaaa tacaccgtcg gctataaggc ggttaatggt ttcgcgccgt
    16201 tttagctcca aatcgccgat ggtgaatgtg ggttcaatgt caataatatt taggctgtag
    16261 ccgtaaacct catgaaaact tacctgtgcc agcaccataa ccttaaggcc gggagaaagc
    16321 ttacggccag ttgtggattc aaagtagggt ttaagcatgc ggtagctgta cgaccatatt
    16381 gtggcccttg cacgtgccac aatgttgtcc gattcttcgt ccttttggat gagttcaagg
    16441 tagcaatgtc ccgagtaatg ctcctttatt tcgctaattt cacccacaat ccagtatgcc
    16501 tgcgacaagt attggcttag ggtttccttt acaagcaggt taagctcctt gagcgagtat
    16561 gttttttgtt tttctaccat cactgtttaa tacccgtaac gtagtatcta ctgcctgaga
    16621 tatctattga gagcaggtaa atccctttgg gcaattcctc agaaatgttg atttcaacat
    16681 gaccagtacc atcgctaaaa gcattcatag ttaaaatcag ctgaccgttg gttgtgaaaa
    16741 ttgatatgtt tgctggtatg aacgattttc ctaaaaggat attaattttt gaagtaaagg
    16801 ggtttggcca aagttctata ctgtttctag catcagtaat gcttgaagtt gatgcctgcg
    16861 caaaatcagg aattccgtaa cccgtgctgt tgttgggtga tgcattgttc gacgatgaac
    16921 ttttaactag ctcaacaatt tgttgtgcgg taagcgatgg tttggcctgc caaaggcaag
    16981 ccaccatgcc agccatcaag ggtgatgaga aagatgtacc attggaagtt ccagttgttc
    17041 cggtagcaat ggccaaaaca gtccctttac ccatggcgca aacatcgggt tttattcttc
    17101 catcggccgt ggggccaaat gagctgaaat cggcctttac tcttttgtta tctactgcac
    17161 ctactgttag aacgtttggc gaatcggcag gaaccgaaat gtagtgccag ggggcgttcc
    17221 catcgtttcc ggcactcaca accacaacca ttcctttgct tgctgccatg ttggcagcaa
    17281 gtgtgacagg ggctgtgttg ccattaagat cctgataggt gtgattttgg ctggcaacat
    17341 caaaagtggt ataacccaac gaggagttaa tgatgtcagc tccaacacta tcggcaaact
    17401 ccgcacctgc agcccaattg tattcctcaa ttatttgctc ggtggccgca tcttcggttc
    17461 taattagcca ataattagcc cttggggctg tgccaattag ttgcccgtca atatatcccc
    17521 ccatggccga tagtaccatt gtgccgtggt aatgctcctt ataaatatcc gattgtgggt
    17581 tcacaaaatc acgagcaccc agtatgcgtc ctctttgcca aaggctatcg aaagcattat
    17641 gggtattaac attcaggaat cctgcatcga gcactgcaat aactttttcc tgaccaaggt
    17701 aaccattggc gtgaagcaag tggccattca tcatagcaat ttgagtgtac gatgcgccgt
    17761 agtttaaccc tgcaaattcc cttttttcat ttgttccaag gggctgccac tccgttttat
    17821 catcggtttc tgactttgct ggttcataaa tcttggttat gttttttaca aagggtaacc
    17881 cttgcacgct tgttagctgc tcgggggtta aagtggcaac aacgccatta aaccagcgta
    17941 gggagtaatg aaccattgtg gcgctctgag caacctggct tacataagct ggcgttacgg
    18001 gtaaatcatc ggtggatatg gcaatgccct gttttgcccg tcgttcaata gcccttgccg
    18061 ataaaaattc atttgggtta tcaacggaat atgttgaatt ctgtttgtca gtaaatgcta
    18121 taaaatattt tgcctgggcg aaagccctta ggggcactaa acccgaaatt ataaaaagta
    18181 aaagaatgat tcttaaatgg ttttggtaat ttttcataac aggcattggt tttagtatcg
    18241 ggttcccatt gagttcatta catcgtcaat tgatggctcc tttaattttc ctgcattctt
    18301 taaaagcgat tctatgtatt tcgattcctt ttcgatgagt tcattttcat tctcggctac
    18361 tccattgaaa tattttatgg tttgcgttat ttttccatcg gtatcgtaaa atttccattc
    18421 gccatgcttt agccctttaa tataagaccc ctctattctt gttgtaccac tggggttaaa
    18481 cactttcata gtaccatcaa tctcaccatt tgtatatggg atgatgcttt tgtttaagcc
    18541 attctcaaaa aactgaacaa ccaaaccatt caggtttcca ttggtgtaac ttgcactttc
    18601 cagaacttta cccgaagggt aaaaacttct agtttttccg tttttcaaac cgttcttgta
    18661 tcgttcttca aaaatcaatt ttccctccat ggagtaatac tgccaaaggc tatccttttg
    18721 ctggtttttg taaaaaccgc taacaatttt tttcccgtct tcgttgaaga aagtagtgat
    18781 tacgtagggt gaagactcgg agtaaaccat tatcaccttt agctctccat tttggtggta
    18841 acgtttaaat tctccaactg gtttgtcgtc cttgaaccaa ccctcatagg ctttcttgcc
    18901 gtttgggtaa tacttgcacc aataaccctg ctttcggttt ttactatcaa gttggttttt
    18961 tgcaccatcg gtttgcccaa tggctaatcc agaaaaaata agtagaacta gaaaagccaa
    19021 tatacgtttc atgcttactg ttttttatta agttctattg caaattgaat gcctttggtt
    19081 agtataacaa attggtttat ggtaaagtgc aagatttata agttaaaatt gtcaacaaat
    19141 caaaaagttg atgcaatata aatttttagt taaactcgta tattgccatt aaacaaaaca
    19201 cttgatatta accttctttt tctcatttat cagctgtaat aagttcaaag ataagctcag
    19261 tcagcgttgt aacatcgctt tcaacagttc ctttcatata acgttcgtaa acacttatat
    19321 tgtctggtgg gttgagattt aatatcaagc ccttttccaa tgccaacagc cgaataaatt
    19381 ctctttgtgc tcgtccattt ccttctctga agggatgcaa atagttaaca ttgtctaata
    19441 tttctgccaa tttttctgct aatttccttt tgttgttttt cgggattttc ctgtactcat
    19501 caattagttg gtcaatatac ctaaaagcgt tgtcaaagta agtagttggg aagaattgtt
    19561 ttccgtcttt gctgatttca acaacccgtt ttttcccagc ccaaatgtaa atatcttgaa
    19621 acaaatgtct gtgaatttca aaaaggctgt caattccctt aatttttatc gggttctcgt
    19681 aaagttccaa aagtcgttta gtcaccgcac tactctcaac aaagagtaat acttcggggt
    19741 ctgatatatc ctgtaaattt cttaaaattc ctgtcttggg gtcaatgtag ctgtagtctg
    19801 ggtctatgta cttgtaagaa ttagacatag gctttttgtt ttgcaaatgc cacaagttct
    19861 gttagggata tttttccagt cacataatcc ctaataattt caattccttt ttgagttggt
    19921 gtaaaacctt caaacatgtt actccccaaa gcccttgctg tgagttctaa agttttcagg
    19981 tccgaaaatt ttacccccat tattgtcaga ttctttctgt ctacttcaat tgcattgaac
    20041 atctcttttc agttcttaaa tttcctcaaa tttacaaaaa atcttcgtca aatctttaat
    20101 tttatagtat tagaagggtc tataggaaac agtataaaat gaaaaaagcc gccaattagg
    20161 cggctttggt tagtatccat tcgggttatt tcacctcttc aaaatcaaca tcagttactt
    20221 cctggtcgtt gcccgatgat gacgagctac ctgcgttggc attggcctgt tggccagcac
    20281 cagcgttagc ttgtgcttgc gctttgtaaa gttcctccga tgcagcctgc catgcagcat
    20341 tgagttcgtt ggttgcgttc tcaatggcag ctaaattctg gctcttatga gcgtccttaa
    20401 gtttaccaag tgctgcttca atggaagctt tcttgtcggc agggagttta tcgccaaact
    20461 ctttgagttg cttttcggtt tggaaaatca agctgtcggc agcgttaatc ttctcgatac
    20521 gttctttctc gcggcggtcg gcttcttcgt tgcgtttagc ctcctcgcgc atgcgcttga
    20581 tttcctcttc ggtaagcccc gatgaggcct caatacgaat tttctgctct tttccggtac
    20641 ctctatcctt agcggttaca tgaagaatac cgttagcgtc gatatcaaaa gtaacctcaa
    20701 tttggggtac tccacggggg gcgggtggta ttccgtcgag gtggaaacgt ccgattgtct
    20761 tattatcctt tgctaatgga cgttcgcctt gcagcacgtg aatctcaacc gaaggctgat
    20821 tgtcggcagc agtggtaaac acctcgcttt tgcgggtagg aatggtagtg ttagcttcaa
    20881 tcagtttggt gaacacaccg cctaaggttt cgatacctaa cgaaagcggg gtaacgtcca
    20941 agaggagcac gtccttaacc tcgccggtta aaacaccgcc ttgaattgca gcaccaatag
    21001 caacaacctc gtcggggtta acgctcttat tgggcttgcg gttaaagaat ttttcaacaa
    21061 cctcctgaac tttagggata cgggttgagc cacctacaag tatcacctcg tcgatatccg
    21121 atggttgtaa acccgcatcg gccaaagctt tacggcaggg ttcaacggta cgctgaatca
    21181 gatggtcggc taacttttcg aactgagccc gggttaatgt ttttaccagg tgtttaggta
    21241 tgccattaac gggcataatg tacggtaggt tgatttcggt tgtggtagtg ctggagagtt
    21301 caatctttgc cttttcggca gcttctttta aacgttgcag cgccatggga tccttgcgga
    21361 ggtcaacccc ttcgtcttta aggaactcct cggcaagcca atcgatgata acctggtcaa
    21421 aatcgtcgcc tccaaggtgg gtatcgccat tggtcgattt tacttcgaaa acgccatcgc
    21481 caagttcaag gatagagata tcaaatgtac ctccaccaag gtcatacacg gcaaccttga
    21541 tatccttatt ctttttatca aggccgtagg ccagtgcagc agcggtaggt tcgttaatga
    21601 tgcggcgaac ctttaagccg gctatctcgc cagcctcttt ggtagcctgg cgctgcgagt
    21661 cgctgaagta tgccggaacg gtaataacgg cttcggttac ttcgtgtccc aggtaatcct
    21721 cggcggtctt cttcattttc tgaagaatca tcgatgaaat ttcctgaggg gtgtacaggc
    21781 ggc
//
LOCUS       DAOOWJ010000037        21217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2487_length_21217_cov_8.123618, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000037 DAOOWJ010000000
VERSION     DAOOWJ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 21217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21217
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_21217_cov_8.123618"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1129)
                     /locus_tag="PLW22_05330"
     CDS             complement(38..1129)
                     /locus_tag="PLW22_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOU74161.1"
                     /translation="MKKILTISVLAAFVFSVAAQNAVPKINFKTSTYDYGKIKEDDGV
                     ANYSFEFTNTGNAPLIIQRVATSCGCTTPEWPKEPIAPGKGGKITVGYNPAGRPGPFA
                     KTVTIYSNAETPTVILQIKGEVIPHVKTIDEIYVTPIGDELKFEKIHFALGRIFLGST
                     YNDTIRFINKSSAPVTVKANTQGMNFMQVKVVPEVVKPNQFGAIVLTYNPDKREEWGF
                     VVDRFTISVNDKAMNTSPITITASIEEDFSKLTQKDLENAPKAEFNTLQYDFGTVKSG
                     DPVNYDFVLKNTGKTDLIIRKIKASCGCTTATPSKSVLKPGESTEISASFRTAGYSGR
                     QGKTITVITNDPRNSTLILRLTGNVDKIE"
     gene            1268..1636
                     /locus_tag="PLW22_05335"
     CDS             1268..1636
                     /locus_tag="PLW22_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOU74162.1"
                     /translation="MSIAHLVLLLLIGLTAGFVGGALGLGGGIIIVPALIYLLNFSQH
                     EAQGTSLMVLLFPVGILAVVNYYRAGFIDWKLALILMVGVVIGSYFGSKVSINIPAET
                     LRKIFGFFIVLVGIKMILGR"
     gene            1633..2793
                     /locus_tag="PLW22_05340"
     CDS             1633..2793
                     /locus_tag="PLW22_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOU74163.1"
                     /translation="MISKSDTENILVEYRRHLHKHPELSFQEVKTSSYIKQIFATEGV
                     RVEDFGTHHSFAAIVEGKLPGKTYGLRAELDALPITETSGVAFASVNPNVMHACGHDM
                     HMAIAIGVTLSLYRQRDKLKGRFVAIFESGEEVLPGGAQAIIQTKLYNELKPDAMFGV
                     HVLPELEVGKIGVCPGKYMASGDEVYITVKGKGGHAALPHLLIDPITCAAEIVLGLQT
                     LVSRKAPTLIPTVLSFGRIEANGATNIIPDNVSISGTFRTMDEQWREEAHKLIQQTAT
                     GIAASHGAKCEVEIRKGYPSIFNNPELTQLVKQTATELLGNDKVVDLEPRMTTDDFAY
                     FSTLTPSVFIRLGAGFPGSDNPQLHRPNFNPSEGAIGIAHSLLTRILLTLMK"
     gene            2798..7033
                     /locus_tag="PLW22_05345"
     CDS             2798..7033
                     /locus_tag="PLW22_05345"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOU74164.1"
                     /translation="MTLGRNFLLALFFTLFQFKTLYSQSEFSKVETDYYTSFINYYQS
                     DGICSYEFTDIIQDGMGLMWFATQDGLMRFDGNNFKTYRGGKTKGQLPHSVVSSLAVD
                     KKKNLWVGTKGGLCVYHPESDTFERVDSLFGEPLQGHRWVRDIYVDSKGRLWVDRHNG
                     TLNCIDFDAKTVETFQHEYVYNEIYYFHAITELGEGVMLGGGSAHLTWLTRNPDKWNT
                     YPSVETIINNEIITIAGTANFYDDGSDYIWVANYVDNAYRINKKTMERTPLPLPSVYT
                     ICPNGDGKVWMGGYHFGVVLYNPSTNTATRYIRMEGNPQSLAGNMILVIYRDNQGNTW
                     FGTNNGLSLLTTFARKSTHLRKITDVNSLPSNQITCMFPDYPKGLWLGTQNKGLVFID
                     FNTKNVTRFGYFNHPDSLASNWVTSIARTTDNSMLVTLWNGYGGALNRFWPEKKRFKR
                     YNPDGYYWYSNVITTSDGKALFSPWGGRVNEFDPRTENVTSRIFGCFENQCSNVSGYI
                     NKITTSNNGWILFEFATLLNVLNSDTLNIVPSNFTNLDIGIPKQKQVTFNIQAKKMLL
                     FNSNLMILGNDNKIYPFSLPTRKIEPPLSTNINFTNIFADKYLYALTDSVLFRWNQER
                     GRFDQLTSIKQFPLIKSIADGNDTIFLGTSEGVYVMTEHTKTLTKLFDADVQALTFYK
                     GFAFGASNKGLLMFNGKGLVKRYLSEQSITNFTIDSSGNVWATNSEELFRLSLKTNSI
                     ETIGAYPQSKDSLPSVQVKSIATDALGNVWILSDVCISKYLPKQKRYESYYVPGKSAL
                     QSTLVYRMYEDSKGYVWVGYTRNGVGVDRIDRRTHTVDHFPYLPYDSTSYPATSMTHC
                     IFEDANGNMLFGTDAGLAILQPESKAFQMIDTRHGLPSSVVTAIYQDRLGNYWIGTDK
                     GIARFSKKLKRVDITGKNLNFSEGLVTAIEQWGFDSLVVSGNFGLYIFNPYVQGNEVP
                     MQDIKILSCETDYDTLARFPANNSKIFVKANHKWLKIHFTASDYTSPGALEYTYWLQG
                     FEDSTRAKKTHSPLAEYTNLPYGKYTFKVYYTRPDGTKSKNVFAINVVVGMPLYLKPW
                     FFLIFLLLLALSVYIHEILRVRRVKRKKDELEQAVMEKTKAILAQNEELKAQADLIKQ
                     QSNNLKLKTYQINESLEFSKLLQSNLMPSPEILSTILGEHLIFYKPKDVVSGDFYWLK
                     GNSDEFIIAIADCTGHGVHGGFVSMMDITLLNEVYTHYKINNPVELIIETHNHFNQTI
                     GAQLQLNASSAPVEMDIAVCYVNRKNKTALFASTHTPLYHIQSNFENPELKIYKSGGK
                     AVGNRWFTPNIPLVELKLESGDILVLTTDGLFDQLSKSEKKRFGRKQFESMLLENANG
                     NLDTINKKIEQCFDDWRGDSQQTDDITIIGFRI"
     gene            complement(7086..7589)
                     /locus_tag="PLW22_05350"
     CDS             complement(7086..7589)
                     /locus_tag="PLW22_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOU74165.1"
                     /translation="MANAQDHPIKKVMIICAKANLEDVYAALVMANGAVMEGIEAKVF
                     FTFFGLDAITKKQMKRLHTATVGNPGLRMPGGLPFPTLLGMLPGVEAGVSAMMKSQME
                     KLDIPPVDEFLELITAGGGEIYACKLAVEMFGLKKDDFSEHVKDIITIGQFYELAAGE
                     GTQIIFV"
     gene            complement(7608..7919)
                     /locus_tag="PLW22_05355"
     CDS             complement(7608..7919)
                     /locus_tag="PLW22_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOU74166.1"
                     /translation="MAEKVYAGKSVSVTDDGYLTDPNQWTKEVAIEIAKEEGIELTDK
                     HFAVIEFIREKTLKGEALTIRTIGKSGLVDTKGFYELFPGAPLKKASRIAGIAKPSSC
                     V"
     gene            complement(7941..8615)
                     /locus_tag="PLW22_05360"
     CDS             complement(7941..8615)
                     /locus_tag="PLW22_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74167.1"
                     /translation="MENNLQQQIDDLNRKMDLVLELLAKQQQRSEVVEDLVEDLNIVV
                     KDAFQTAVDELAVQNIKIDGEDVRYLVFKLLRNVKTFGELMDMLESLMDFLHDIGPVV
                     HDAGIAFTKALDDLDRKGYFDYFRQLGLLAAEFKEHVTEEDIIRLRESMPAVGLMLRS
                     LTQPKVMNALAEVTQTLSSMEMDEKIDNKSLIGIARQLSKPEVRRTLSFMLRVMGELG
                     KTKKIN"
     gene            complement(8628..9857)
                     /locus_tag="PLW22_05365"
     CDS             complement(8628..9857)
                     /locus_tag="PLW22_05365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOU74168.1"
                     /translation="MKKIVILGAGTGGTIMANKLRKVLERDEWSITVVDKDPNHYYQP
                     GFLFIPFGIYSKHDVVKPKASFIPAGVKFIVNDIDRVEPDKNTVYLTNGQILEYDFLI
                     VATGTHINPAETPGLLGREWRKSIFDFYTHEGAVALSTFLKTWEGGDMVINIAELPFK
                     CPVAPLEFAFLADEFFVKKGIRDKVNIYYTTPLSGAFTKPKSSKMLGELLKEKNIQII
                     PDFYLERVDDENKKIISYDGREVKFDLLTIIPVNMGADWVARSGMGDDMNYIATDKYT
                     LRSEKWENIFVLGDASNIPTSKAGSVVHFASDVLFENLMAAIEGRPLIAKFDGHSNCY
                     IETGFGKGSLIDFNYETEPLPGTYPLPGIGPFGLLKNTRINHYGKVMFRWIYWHLLLK
                     GKELPLESQMTMAGKEL"
     gene            9986..10684
                     /locus_tag="PLW22_05370"
     CDS             9986..10684
                     /locus_tag="PLW22_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU74169.1"
                     /translation="MSQNFCPGNICAECGFRSSVFSKLTDEQLNQLTANKTFHFAKKG
                     ETIAKQDSAIKGFIFLRVGLAKLTRVNPDGREQIIGIATPNDFVGLLSIFSSKNYQYN
                     IIAIEDCEYCCVDYNLVINLISTNGDFAKTLLEKISQVADLMMGTRLDLELRQLRGRV
                     AFIICYFGNEVYKSHKFSLPISRREIAELIDMRVENVVRILSEFRRDNIIRIEGTTIE
                     IVDPEKLRWIKMHG"
     gene            complement(10686..11435)
                     /locus_tag="PLW22_05375"
     CDS             complement(10686..11435)
                     /locus_tag="PLW22_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU74170.1"
                     /translation="MITVKNLNKSFDGRLVLEGINATFEKGKTNLIIGQSGSGKTVLL
                     KCIVGLHEVDSGEIYYNDTRFDNLNFRDRKKIRQKIGMLFQGSALFDSATVEENVMFP
                     LDMFTTMSHEEKLDRVNFCLNRVNIINSNHLYPAEISGGMKKRVAIARAIALNPEFLF
                     CDEPNSGLDPKTAVVIDQLIKEITEEFNITTIINTHDMNSVMGIGDKVIFIHNGKKWW
                     EGSKENILDSDNKELNDFVFSTELTRKIKGL"
     gene            complement(11432..12181)
                     /locus_tag="PLW22_05380"
     CDS             complement(11432..12181)
                     /locus_tag="PLW22_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU74171.1"
                     /translation="MKLLFAIDMFGEYILLLKKVFSKPEKRRVYLQQTIKEIDKLGLQ
                     SIGIVTIISVFMGAVITLQTAYNMENPFIPNYLIGLGTRDSMLLEFSSTIIGLILAGK
                     VGSNIASEIGTMKVTEQIDALEIMGINSASFLILPKIIATILFNPFLTVLSMIVGIAG
                     GWLVGVFTGVVSTQEYIDGVQYAFIPYYITYSLIKTVVFAFLITTISAFYGYRVQGGS
                     LEVGRASTQAVVMSSIFILLFNLILTQLLLS"
     gene            12467..13555
                     /locus_tag="PLW22_05385"
     CDS             12467..13555
                     /locus_tag="PLW22_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOU74172.1"
                     /translation="MIERKNLYCVIMAGGIGSRFWPLSRVDKPKQFIDILGTGKSLLQ
                     QTFDRMSRVCPRENILIVTSSAYSSLVNEQIPNLSPDQVLLEPLRRNTAPCIAYATYK
                     IKQQNPNAIAIVAPSDHLILNEDIFLDTILKAAEFASNSNALITLGIRPSRPETGYGY
                     IQIGKPVKEVPSLFKVKTFTEKPNLELAQKFLESGEFYWNSGLFIWSIASISAALESH
                     LPEVNSLFKELLPIYGTEKETETIATAYSECRNISIDYGVMEKADNVYVFCSEFGWSD
                     LGTWGSLYTHLPHDPNGNAAIAQQLMLYQTSNSIFNIPKNKLAVIQGLDDYIVVDTKD
                     VLLICKKQDEQQIRNFVNDVLMNNKDFA"
     gene            complement(13627..>14028)
                     /gene="gldN"
                     /locus_tag="PLW22_05390"
     CDS             complement(13627..>14028)
                     /gene="gldN"
                     /locus_tag="PLW22_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOU74173.1"
                     /translation="VKEDWFFDKQRSVMEVRIIGICPILHRTDYRKTGSDEEQEGTLS
                     RIPTFWIYFPEARSVLANTDAYNNFNDAQRITYDDLFWKRNFDSYIIRISNVWDNRAI
                     DEYTFTGLQTLLESEKIKTELFNFEHDLWEY"
     assembly_gap    14029..14128
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14129..15354)
                     /gene="gldM"
                     /locus_tag="PLW22_05395"
     CDS             complement(<14129..15354)
                     /gene="gldM"
                     /locus_tag="PLW22_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOU74174.1"
                     /translation="MMYLVLTAMLALNVSSEVLEAFVLVDNGLSRTTESYRLKNKKLY
                     DEFAAAYSINPVKVGPYKQKADEIQKKATEILQYVQDLKIEVVKASEGEETPAIQNGE
                     IIAELIQGKSDLDKAATILIGFEGNGKAKVLKQKLEEFREYIVSIIDKDKAPELVESI
                     KKILNTDDPPVKEDGTHNTWESARFEHIPLIAVIPQLTKVQVDILNVEAEIINYLMGS
                     IGAAEFKFNKLSATVIPNSNIIFQGSEFRAEIFLAASDTTQVPKVYLCRYDSVFNSAI
                     NGYDYRPIGNTEEVPVNKTGRAVLTRKASSTGVVNWQGLIEMKAPDGTIVRRPFKHSY
                     TVMPPNVVIAPTKMNVLYLGVENPIDISVPGFAPDKVSATISRGSIKPVGMGSYIAQP
                     GAGAPTVEITVFAETEG"
     gene            complement(15429..16670)
                     /gene="gldL"
                     /locus_tag="PLW22_05400"
     CDS             complement(15429..16670)
                     /gene="gldL"
                     /locus_tag="PLW22_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOU74175.1"
                     /translation="MKISIEEIVTSKKWKTFMKYLYGWGASVVILGALFKILHLKGAG
                     TMLFLGMGTESIIFFFSAFEPIHEEVDWTLVYPELTGMSDEEEISGYRRSRNQGLSAE
                     DVQQIITSVLASLPAGSAGVAAAAPASAAPASQPAAVGVPGALIFTEKFNKMLENAEI
                     SPALFDKVSAGLNKLGDASSKIADITNSATVVKEFNEKLTKASESVGTFTQNYSQSGQ
                     ALNESINVLNQNIQQAAGVMSESGKNFMDGVNQSVKNLEAELTNAGQKVSERIVGSAE
                     SVADNLKVSADGLANTYKQTASQVATTYQQLAEAMQLNGNVITNGSTSYKEQLERLNK
                     NMAALNAAHELHLQETSQRLKEAEKVYQGVDGMVKKLNFTIEETEKFRLAVEKLNSNV
                     AALNNVYGNMLTAINALSNGK"
     gene            complement(16707..18035)
                     /locus_tag="PLW22_05405"
     CDS             complement(16707..18035)
                     /locus_tag="PLW22_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU74176.1"
                     /translation="MKKFLSIGILASLLYGCGPGNTGELTGVPGRRSFSEPTPLGMVF
                     IPMGSFTLGSNDQDVAWAINAPTKTVSVDAFWMDQTEITNNQYRQFVYYVRDSIARRL
                     LGEINDEFLITEDQFGNPLDPPVLNWEVPIDMEDEEQREALNALYYSKEDEFFGRKEV
                     DTRKLFFEYYWIDIKQAAQKRNRYNYETLSYDQGEVINSLGEKVKVDNRQSFIMRDKV
                     NVYPDTLCWVADFTYAFNEPYTNSYFWHPAFDNYPVVGVTWKQATAFCIWRTQTLNKF
                     LAPEGITIQDYRLPTEAEWEYAARGYLNHSMYPWGGPYTRNMEGCFLANFKPLRGNYV
                     DDGGLIPIPVGTYEPNDYGLYDMAGNVAEWTANAYDESAYSFMHDMNPDYKYNSKPSD
                     LPALKRKVVRGGSWKDISFFLQVGSRTYEYQDSAKSYIGFRCVRSYLGAK"
     gene            complement(18093..19040)
                     /locus_tag="PLW22_05410"
     CDS             complement(18093..19040)
                     /locus_tag="PLW22_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOU74177.1"
                     /translation="MRVFGNLVFATTLSVLLSSHAFGQQDPQFNQILFNPLTINPGYA
                     GSQNNMIGVGAISRIQWAGFGDGAPLSNTLGINAPISPFGFKSGVGLSILNDSYGFNN
                     DLGINIAYAARFKFKLINGDLGFGVGAGFVNNKLDPKWNYPVGGGDEAIPDQKESSIN
                     FDLSAGLYYSTEYMFFGFSALHLNETKTNKAALPARYTRQYYLTGGYIVNFPNPTWQF
                     EPSVIVHSDLKSSKLSLNTIVRYNKKVWGGVSYRVGEAITGLVGFELFNGIKFGYAYE
                     FSTSKISKYNNGSHEFYMGYNFQLNKEKPPQQYKSLRFL"
     gene            complement(19266..20423)
                     /locus_tag="PLW22_05415"
     CDS             complement(19266..20423)
                     /locus_tag="PLW22_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOU74178.1"
                     /translation="MLANELIAYLAPSDVTGIYPFAEKKYCFGNQVINLHDQPDSPIR
                     PGDIFIVGIESIDSDSVTNNIRQMLWGLSSIHYSKGKVFDAGNISATLTLAQQYQVVE
                     SISAIIAESKAHLILLGGSQEFLSKVYAGWKQIQSFIRMAIIDSKIDWDGNNNDFHQD
                     NFVNTLIDEPYEKLLDLSFIGIQGYFTPQHTLQKVLRRKHDYIRLGNLRGNIREAEPT
                     LSDANLVSIDMAAIRASDAPGKAFDNPNGLYAEEICMLSRYAGIGQSSKFLGICGLKT
                     NAPEQTIMLAAQVLWHYIEGVSHRRVENPIKNLPHNKKYVVSTEVQNVEMIFFRSELS
                     GNWWFELPVNSKKKLLRGKLLVRCNASDYSQASNGEIPQRWLRWYQKAQKI"
     gene            complement(20432..>21217)
                     /locus_tag="PLW22_05420"
     CDS             complement(20432..>21217)
                     /locus_tag="PLW22_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HOU74179.1"
                     /translation="PSDIGIVVNDFLVEYFPNIVNYNFTAQVEEKFDEIAMGKMEWTK
                     MIDEFYKPFHDTVKKTEKESDYTKGERVLGTDPATGKTVSVRIGRYGPVVQLGDNDPN
                     SKEKPQYASLLKGQLVSTITLEEALSLFKLPRTLGSFEGKEVVVAVGRFGPYVRHAGK
                     FYSLKKDDNPLTIDLNRAIEIIEEGRLKEKQRVIKTFTEDPELMVLNGRWGPYISKAK
                     SNYKIPKGTNAAELTYNDCLKIIEESEKKETTGKKGTKTTKKK"
BASE COUNT         6102 a         4597 c         4258 g         6160 t
ORIGIN      
        1 cccccaaaaa aaagccggct taacaccggc tttattgcta ttctatctta tcaacatttc
       61 ctgttaggcg gagtattaat gttgagttac ggggatcgtt ggtaataact gtaatggttt
      121 taccctgtcg gccggaatat ccagctgttc ggaacgaagc tgatatctca gtggattcac
      181 caggcttaag aacactcttg gatggggttg cagttgtaca accacagctt gccttaatct
      241 tgcggataat taaatcggtt ttacctgtat tcttgagaac aaaatcgtag tttacggggt
      301 cgcccgattt aaccgttcca aaatcgtact gtagggtatt gaactcagcc ttgggcgcat
      361 tttccaaatc cttctgcgta agctttgaaa aatcctcctc aatgcttgcg gtaatggtta
      421 ttgggctggt gttcatagct ttatcgttaa cagaaatggt gaacctatca acaacaaagc
      481 cccactcttc gcgcttatcg gggttatagg tgagcacaat agcgccaaac tggtttggtt
      541 taaccacctc gggtaccact ttcacctgca taaagttcat tccctgtgtg ttggccttaa
      601 ctgttacagg tgcacttgat ttattaatga acctaatggt atcgttatag gtactgccta
      661 agaatattct acccaaagca aaatgaattt tttcgaactt aagttcgtcg ccaatagggg
      721 ttacatagat ttcgtcaatg gttttaacat gcggaataac ctcgccctta atctggagaa
      781 ttacggttgg cgtttcggca ttggagtata tggttacggt ttttgcaaat ggccctgggc
      841 gtccggctgg attgtatcct acagttattt tgcctccctt cccgggtgct atgggttcct
      901 taggccattc aggggttgtg catccacacg atgtagccac acgctgaatg attagtgggg
      961 cattacctgt attggtaaac tcaaaggaat agttggctac cccatcatct tcctttattt
     1021 taccgtaatc gtaggtactt gttttaaagt tgatttttgg aactgcattc tgcgctgcaa
     1081 ctgaaaatac aaaagcagct aaaactgaaa ttgtcaatat ctttttcatg atcaatatgt
     1141 ttgaaacgtt tacaattttc tacgtacaaa gttataaaac gggtttctaa aatcgcctta
     1201 aatgtaaatg ttttatcatc aacatatatt ccaaaaacat tactaaattt gttaaaaatg
     1261 ttttaaaatg agtattgcac accttgtttt gcttttgctt attgggctaa ccgccggttt
     1321 tgtgggtggt gctttaggcc ttggcggagg gataattatt gttccagctt taatctattt
     1381 gctaaatttt tcccagcacg aggcgcaggg caccagcctt atggtattac tctttcccgt
     1441 gggaattttg gctgttgtaa actactaccg tgctggattt atcgattgga aacttgcact
     1501 gattctaatg gtaggtgttg taattggcag ctactttggc tcaaaggtat ccattaacat
     1561 cccggccgaa acactccgga agattttcgg gtttttcatt gttttagttg gtattaagat
     1621 gattttaggg cgatgatttc aaaaagcgat actgaaaaca tactggtgga ataccgccgc
     1681 caccttcaca aacaccccga actctcgttt caggaggtaa aaacatcaag ctacatcaaa
     1741 cagatttttg caaccgaagg agtgagggtt gaggattttg gtacacatca cagctttgca
     1801 gcaatagttg aaggcaaatt gcctgggaaa acatacgggc taagggcgga actcgatgcg
     1861 ctaccaatta cagagaccag cggtgttgct tttgcttcgg ttaaccccaa cgtaatgcat
     1921 gcttgcggcc acgatatgca tatggctatt gccataggtg ttacgttaag cctttatagg
     1981 caaagggata aattaaaagg acgttttgtt gcaatatttg aatcgggcga ggaggttttg
     2041 cccggtggag cgcaagccat aattcaaact aagctataca atgaacttaa acccgatgcc
     2101 atgttcggcg ttcatgtact tccagaactt gaagttggga aaattggtgt ttgccccgga
     2161 aaatacatgg cctcgggcga cgaggtgtac attactgtaa aagggaaggg agggcatgct
     2221 gccctacccc acctgcttat cgacccaatt acatgtgctg cagaaattgt ccttggcttg
     2281 caaacactgg ttagccgaaa agctccaacg ctaataccca ccgtgctatc gtttggccgt
     2341 attgaggcca atggcgctac aaatattata cccgacaatg ttagcatttc cggaaccttc
     2401 cgcacaatgg atgagcaatg gcgcgaggag gctcataaac ttatacaaca aaccgcaacc
     2461 ggaatagcag catcacatgg tgcaaagtgc gaggttgaaa tccgtaaggg ctatccatca
     2521 atattcaaca accccgaact aacacaactt gttaagcaga ctgctactga actgcttgga
     2581 aatgataaag tggttgacct tgagccccgc atgaccaccg acgattttgc atacttcagc
     2641 acccttacac catcggtatt cattaggctt ggtgctggct ttccaggttc tgataatcca
     2701 cagcttcata gacccaactt taatccctcc gagggggcca ttggcatagc ccatagcttg
     2761 ctcacaagaa ttttactaac cctaatgaag tagatctatg actttaggta ggaatttttt
     2821 gttagcctta ttttttactc tctttcaatt taaaacgctt tactcacaat cggaattcag
     2881 caaggtagag acagactact acaccagttt tatcaactac taccagtccg atggaatatg
     2941 ctcgtacgaa tttactgata ttatccagga cggaatgggg ctgatgtggt ttgccacaca
     3001 ggatggacta atgcgatttg atggtaacaa ttttaaaacc taccggggtg gaaaaacaaa
     3061 aggtcaatta ccgcacagcg ttgtaagttc acttgctgtg gataagaaga aaaacctttg
     3121 ggttggcact aaaggcggac tatgtgttta ccatccagaa tccgacacct ttgaacgggt
     3181 tgattcccta tttggggaac cgctgcaggg acaccgttgg gtaagagata tctacgtgga
     3241 tagtaaagga aggttatggg tagataggca caatggaacg ctcaattgca ttgactttga
     3301 cgctaaaact gttgaaacct ttcagcacga atatgtctac aatgaaatat actacttcca
     3361 cgccataacc gaacttggcg agggtgtaat gctgggaggt ggttctgctc atcttacctg
     3421 gttaacccga aatcccgata aatggaatac ttaccctagt gttgaaacca ttataaataa
     3481 cgaaataatt actattgcag gaactgcaaa tttttacgac gatggctccg attatatctg
     3541 ggtagcaaac tatgttgata atgcataccg aatcaacaag aaaaccatgg agcgaacccc
     3601 acttccccta ccatcggttt ataccatttg cccgaacggc gatggaaagg tatggatggg
     3661 tggctaccac tttggggttg tactttacaa cccatcgaca aacacggcta cccgctacat
     3721 taggatggag ggtaatccgc aatcgctggc aggtaacatg atcctggtaa tttaccgcga
     3781 taatcagggg aacacatggt ttggcaccaa caatggttta tcgctgctaa caacctttgc
     3841 ccgaaaatcg actcatctcc gaaaaattac cgacgttaac agcctgccat caaaccaaat
     3901 tacctgtatg tttccagact acccaaaagg gttatggttg ggtacccaaa acaagggact
     3961 tgtattcatt gattttaaca ccaaaaatgt tacgcgattc gggtatttta accaccccga
     4021 tagccttgca tcaaactggg ttacatccat tgccaggaca actgacaact caatgcttgt
     4081 taccctttgg aatggatatg gtggggcgtt aaatcggttt tggcccgaaa aaaaacggtt
     4141 taaacgatac aatccagatg gttactactg gtactccaac gtaataacaa catccgatgg
     4201 caaagcgctt ttttcaccat ggggaggaag agttaatgaa tttgacccaa gaaccgaaaa
     4261 cgttacttct agaatttttg gttgttttga aaaccaatgc tctaacgttt caggttatat
     4321 caataaaata acaacttcta acaacggatg gattttattt gagtttgcaa cactattaaa
     4381 tgtcttaaac tccgatacac tcaacatagt tcccagtaac tttactaatc tagatattgg
     4441 tattcctaag caaaagcaag ttacgttcaa tattcaggca aagaaaatgc tattattcaa
     4501 tagcaattta atgattttag gtaacgataa taaaatatac ccttttagct tacctacccg
     4561 taaaattgag ccccctcttt ccactaatat caactttaca aatatttttg ccgataagta
     4621 tttatatgcc ctaaccgata gtgtattatt ccggtggaac caagagcgag ggagatttga
     4681 ccagttaacc tctatcaagc aatttccgct aataaaatcg attgccgatg gcaacgatac
     4741 aatttttctt ggcacatcgg aaggtgtata tgtaatgaca gaacatacta aaactcttac
     4801 caaactattc gatgccgatg ttcaggcgct tactttctat aaaggatttg cttttggagc
     4861 aagtaacaag gggcttttga tgtttaatgg caaaggatta gtaaaaagat acctgagcga
     4921 acagagtata accaacttca caattgatag tagcggaaat gtttgggcaa ccaactcgga
     4981 ggagttgttc agactatcgc ttaaaaccaa tagcattgaa accattgggg cgtatccaca
     5041 aagtaaggat agcttgccat cggttcaagt gaaatcaatt gctaccgacg cattgggcaa
     5101 tgtttggatt ctatcggatg tttgtatatc aaaatatcta cctaaacaga agcgatacga
     5161 gagctactat gttccgggca aaagcgcact tcagtcgacc cttgtttaca ggatgtatga
     5221 ggactccaaa ggttacgttt gggttggata cacaaggaat ggtgtaggag tcgataggat
     5281 tgaccgaaga actcataccg ttgatcattt cccataccta ccctacgatt caacatcgta
     5341 cccagccaca agcatgaccc actgtatatt tgaagatgcc aatggaaata tgctctttgg
     5401 aaccgatgcc ggattagcca ttcttcaacc ggaaagcaag gcttttcaga tgattgacac
     5461 ccgacatggg ctcccatcat cagttgttac agccatttac caggatagac tcgggaatta
     5521 ctggattgga accgataagg gcattgcccg ttttagcaag aaactcaaac gggtcgacat
     5581 aacaggtaaa aaccttaatt tcagtgaggg tttagttact gccattgaac aatgggggtt
     5641 cgactcgtta gtagtgagcg gtaactttgg cctgtatatt tttaatcctt atgtacaagg
     5701 aaacgaagta cccatgcagg atattaaaat tttatcgtgc gaaactgact acgatacatt
     5761 agctcgcttt cctgctaaca attcaaaaat ttttgtaaaa gcaaaccaca aatggcttaa
     5821 aatccatttt actgcatcgg actacacatc acccggagca ttagagtaca catactggct
     5881 tcagggcttt gaggattcaa caagagccaa gaaaactcat tcgcctttgg ctgaatacac
     5941 aaatctcccc tatggtaaat acaccttcaa ggtttactat acccggcccg acggcactaa
     6001 atcaaaaaac gtttttgcaa tcaatgtggt tgttggaatg cctttatacc tcaagccatg
     6061 gttcttccta atctttctat tactcttagc cttatcggtt tacattcatg aaatactccg
     6121 tgttcgaaga gtgaaaagaa aaaaggacga acttgagcaa gccgtaatgg agaaaacaaa
     6181 ggcgatttta gcacaaaacg aagagctaaa agcacaagcg gatctgatta aacagcaaag
     6241 caacaattta aaactaaaaa cataccagat aaacgagagc cttgagtttt caaagttact
     6301 gcaatccaac ttaatgccaa gccccgagat tttgtcaacc attttaggtg aacacctaat
     6361 cttttataaa cctaaggatg tggtaagcgg cgatttttac tggctaaaag gaaactccga
     6421 tgaattcatt atagctattg ccgattgtac tggtcatgga gtacatggtg ggtttgtaag
     6481 catgatggat ataacacttt taaacgaggt ttatacccac tacaaaatta ataatccggt
     6541 tgaactgatt attgaaaccc acaaccactt taaccagacc attggtgccc agctgcagct
     6601 aaacgcttcc agcgctcccg ttgaaatgga tatagctgtg tgctatgtaa atagaaaaaa
     6661 taaaaccgca ctcttcgcaa gcactcatac ccccctgtat cacattcaga gtaatttcga
     6721 aaatcctgaa ctgaaaattt acaagtcggg cggcaaagca gtaggaaacc gatggttcac
     6781 cccaaatatt ccacttgttg aattaaaatt agaaagtggc gatattttag tacttaccac
     6841 cgatggcctt ttcgatcagc tatcgaaatc cgaaaagaag cgatttggtc gtaaacagtt
     6901 tgaaagcatg ctcctggaaa atgccaatgg caacctggat actatcaaca aaaagattga
     6961 acaatgcttt gatgactggc gaggcgatag ccagcaaacc gatgatatta caataattgg
     7021 ttttaggata tagcaaaaga gcccccttat tggaggctct taacttttga tggaatcaga
     7081 tattcttaca caaaaattat ttgcgttcct tcgccagctg ccaactcgta gaattggcca
     7141 atggtaatta tatcctttac atgctcgctg aagtcgtcct tcttaaggcc aaacatttca
     7201 acagcaagct tgcaggcata tatctcgcca ccgccagcgg taatgagttc aagaaactca
     7261 tccactggag gaatatccag tttctccatt tggctcttca tcatagccga aacaccagct
     7321 tcaactccgg gaagcattcc cagtaaagtt gggaagggta aaccgccggg cattcgtaaa
     7381 cccggattac ccacagtagc ggtgtgcaac ctcttcatct gctttttggt gatagcatcg
     7441 aggccgaaaa aggtgaaaaa caccttagcc tcaataccct ccataactgc gccgtttgcc
     7501 ataaccaagg ctgcataaac atcctccagg tttgccttgg cgcatattat catcaccttc
     7561 ttaatggggt gatcctgtgc atttgccata tttaactgtt tttagggtta tacacaacta
     7621 gatggttttg caattcctgc aatacggctg gcctttttca ggggtgcacc tggaaagagt
     7681 tcataaaaac cttttgtatc aaccaacccc gatttgccaa tcgtgcgaat ggtgagagct
     7741 tcgcccttga gggttttctc cctaatgaac tcaattacag caaagtgctt atcggtaagt
     7801 tctatacctt cctcctttgc aatttcaata gcaacctctt ttgtccattg atttggatcg
     7861 gtcaggtaac catcgtcggt tacgctaacg gatttgcctg cataaacttt ttctgccata
     7921 gctttatttg tttttggtga ttaattaatt tttttagttt tcccaagttc gcccattacc
     7981 cttaacataa acgagagagt acgacgaact tcaggtttgc ttagttgacg ggcaatgcca
     8041 atcaacgatt tattgtcaat cttttcgtcc atttccatgc tggaaagggt ttgggttacc
     8101 tcagccaagg cattcattac cttgggttga gttaaactac gtaacatcaa ccctactgct
     8161 ggcatgctct cacgaaggcg aataatgtcc tcctcggtaa catgctcctt gaactcggca
     8221 gcgagtaggc ctaactgtct aaagtagtcg aagtatccct tcctgtctag gtcatccagc
     8281 gcctttgtaa aggcaatacc ggcatcgtga accacaggcc caatatcgtg gaggaaatcc
     8341 attaagctct caagcatatc catgagctcg ccaaaggtct tcacattgcg aagcaatttg
     8401 aaaaccaaat accgaacatc ttccccatca atcttaatat tctgaacagc cagctcatcc
     8461 acagcggtct ggaaagcgtc ctttactaca atgttcagat cctccaccaa atcctcaacc
     8521 acctcgctgc gctgctgctg cttggccagc aactccagca caagatccat cttgcggtta
     8581 aggtcatcaa tttgttgttg caaattattc tccatggcca aacagtttta aagttcctta
     8641 ccagccatag tcatttgcga ttcaagcggt agctctttac ctttcaagag cagatgccag
     8701 tatatccaac ggaacataac ctttccgtaa tgattaatcc gggtgttttt aagtaagcca
     8761 aacgggccaa ttccgggtaa ggggtatgtt ccgggaagcg gctctgtttc gtagttaaag
     8821 tcgatcagcg aacccttacc gaatccagtt tcgatgtaac agttggagtg accatcgaac
     8881 ttggcaatta ggggacgacc ctcaatggca gccataagat tctcgaatag cacatccgaa
     8941 gcaaagtgaa ccactgatcc ggcctttgat gttgggatgt tgctggcatc gccaagcaca
     9001 aaaatattct cccatttctc ggaacgtagg gtgtacttgt cggtggcaat gtagttcatg
     9061 tcatcgccca taccgctgcg agccacccaa tcggcaccca tgttaactgg gattatggtt
     9121 agcaggtcaa atttaacctc acggccatcg tatgaaataa tctttttgtt ttcatcatca
     9181 accctttcaa ggtaaaagtc gggtatgatt tgaatgtttt tctccttaag gagttcccca
     9241 agcatttttg aagatttagg cttggtaaaa gcacctgaaa ggggtgtggt atagtaaatg
     9301 tttaccttat cgcgaattcc tttcttcaca aagaactcat cggcaaggaa tgcaaactca
     9361 agcggtgcca ccggacactt aaagggcaat tcggcaatat tgattaccat atcgccacct
     9421 tcccatgttt ttaggaatgt gctcaaggca actgctccct cgtgggtata aaagtcgaaa
     9481 atactcttac gccattccct accaagcaaa ccgggtgtct ctgcgggatt aatatgcgta
     9541 cctgtggcaa ctattaaaaa atcgtactcc agaatttgcc cgttggttag gtaaactgtg
     9601 ttcttgtcgg gttcaacacg gtcaatatcg tttacaataa actttacgcc ggcaggaata
     9661 aagctagcct taggtttaac cacgtcgtgc ttgctgtata tcccaaaagg tataaacagg
     9721 aaaccgggct ggtagtaatg attcgggtcc ttatccacca cagtaatact ccattcatcg
     9781 cgttccaaaa ccttacggag cttattggcc ataatggttc cacctgtacc tgctcctaga
     9841 atcacaatct tcttcatatg ttaattatta aattttgctt aattgttaaa attttatcaa
     9901 ttcaaagtac gttaatagta acaaaagttt tagatttaca gctgttgata aaaatcaatt
     9961 ctgatatata tcaatatatg tagatatgag ccaaaatttt tgtccgggca atatatgtgc
    10021 tgaatgtggt ttccgttctt cggttttttc aaaactgacc gatgagcagc tcaatcagct
    10081 taccgcaaat aaaacctttc actttgccaa aaagggcgaa acaatagcca agcaggatag
    10141 cgcgataaaa ggattcattt tcttgcgtgt agggctagca aaactaaccc gtgtaaaccc
    10201 cgatggacgt gagcaaatca taggtattgc cactccaaac gactttgtgg gcttacttag
    10261 cattttttca tcaaaaaact accagtacaa cataatagcc attgaggatt gcgagtattg
    10321 ctgtgttgac tacaacctgg tcattaactt aattagcacc aatggcgatt ttgcaaaaac
    10381 acttctggaa aagatatcgc aggtggccga tctgatgatg ggaacccgat tggatttgga
    10441 gcttcgtcag ctccgtggca gggttgcctt tattatttgc tactttggga atgaggttta
    10501 caaatcgcat aaatttagcc ttcccatctc ccgacgcgag attgctgaac taattgatat
    10561 gcgggttgag aatgttgtga ggattctgtc ggagtttagg cgcgataaca ttattcgtat
    10621 tgagggaacc accattgaaa ttgttgaccc cgaaaagtta cgctggataa aaatgcacgg
    10681 ttaagctata accctttgat ttttcgggtc agctctgtgg aaaagacaaa atcgttcagc
    10741 tccttattat cgctatcaag aatattttcc ttgctaccct cccaccactt tttcccattg
    10801 tggataaaaa ttactttatc gccaataccc ataaccgagt tcatatcgtg ggtgtttatg
    10861 atggtagtaa tattaaactc ctcagtaatt tcctttatga gctgatcgat aaccacggct
    10921 gttttaggat ctaaacctga gttaggctcg tcgcaaaaaa gaaattcagg gttgagagca
    10981 atggctcgag caatagcaac tcgtttcttc attcctccgc taatctcagc agggtaaagg
    11041 tgatttgaat tgataatatt aacacggtta aggcaaaagt ttaccctatc gagcttttcc
    11101 tcgtggctca tggttgtgaa catgtccaag gggaacataa cattttcctc cacagtagcg
    11161 gaatcgaaaa gggctgaacc ctgaaaaagc ataccaattt tttggcgtat ctttttcctg
    11221 tctctaaaat tcagattgtc gaatcgggtg tcgttatagt agatttcgcc agaatcaacc
    11281 tcatgcagcc caacaataca ttttagcaaa acggttttac ccgatccgct ctgaccaata
    11341 atcaggttgg ttttcccttt ctcaaaggtg gcgtttatac cttctaaaac caaacgccca
    11401 tcgaacgatt tattaagatt tttaacggtt atcacgagag taagagttgg gttagaatta
    11461 ggttaaacag aagaataaaa atactactca ttactacagc ctgggtactt gccctaccta
    11521 cctccagcga gccgccttga accctatagc cataaaaggc agagatcgtg gtaatcagaa
    11581 aagcaaatac tactgtttta ataagcgaat aggtgatgta atacggaata aaggcgtact
    11641 gaaccccatc gatatattcc tgggttgaaa caacgcccgt aaatacccct accaaccatc
    11701 caccggcaat cccaactatc atacttaaaa cggtaaggaa ggggttgaat agaattgtgg
    11761 caataatctt aggaagtata agaaagcttg ctgagttgat acccataatc tccagggcat
    11821 caatctgctc tgtaaccttc attgttccta tttccgaagc aatattagaa ccaacctttc
    11881 cggctagtat caacccaata attgttgatg agaactcaag caacatgcta tcgcgggtac
    11941 caagtcctat aaggtaatta ggaataaaag ggttctccat gttgtaggcg gtttgaaggg
    12001 taattacagc acccataaaa accgaaataa ttgtaactat accaattgac tgtagcccta
    12061 gcttatcaat ttccttaata gtttgctgaa ggtaaactct acgtttttcg ggttttgaaa
    12121 aaaccttttt cagaaggagt atatactccc caaacatatc aattgcaaaa agcaatttca
    12181 taggtggaaa ttttatttaa acaaactgaa actcctccaa caaagttaat ggtatatcca
    12241 aaaataagta gcatttagta actaatattt caggcaataa ctcaaaatat tgtgatttga
    12301 accatttaac aaatgatttt cttaaatatt aagcaacgca gaaaatattt gttaaacttt
    12361 acggattacg ttaatgactc tctgccatat cgggttgcat tcaaattctt agcagttttt
    12421 ttgtgccaaa aaatgaacta cttttgcaat ttaatttgta aaaacaatga ttgaaaggaa
    12481 aaacctatac tgtgttatca tggcaggtgg cattggaagt cgcttttggc cgcttagccg
    12541 agttgataaa ccaaaacagt ttatcgatat actaggcact gggaaatcgc tcctacagca
    12601 gacttttgat aggatgagcc gggtttgccc ccgcgaaaat atactgattg ttaccagtag
    12661 tgcatacagt tcattggtta acgagcaaat acccaatcta tcacctgatc aggttttgct
    12721 tgaacccctt agaagaaaca cagcaccctg cattgcatat gccacataca agattaagca
    12781 acaaaaccca aatgccatag ccattgtagc tcctagcgat catctaattc tcaatgaaga
    12841 tatttttctc gataccattt taaaagccgc tgaatttgcc tcgaacagta atgccctgat
    12901 tactctgggt attaggccaa gtaggcctga aaccggttac ggatatattc agataggtaa
    12961 acctgtaaag gaagttccct cgctattcaa ggtcaaaaca tttaccgaga aaccaaacct
    13021 ggagcttgca caaaagtttt tggaaagtgg tgagttttac tggaattcag gcttgtttat
    13081 ttggagcatt gcctctatct cagcggcact cgaaagccac ctacctgagg taaacagctt
    13141 atttaaggag ttactaccca tttatggtac cgaaaaagaa accgaaacaa ttgctacagc
    13201 atactccgag tgcaggaata tttccattga ctatggagtt atggaaaaag ctgataatgt
    13261 ttacgttttt tgttccgagt tcggatggtc cgatttggga acctggggct ctctatacac
    13321 tcacctaccc catgacccaa atggaaatgc agccattgcc cagcagctta tgctttacca
    13381 aaccagcaat tccattttca atatccccaa aaataagctt gcagttattc agggtttgga
    13441 cgactatatt gtggttgata ccaaggacgt tctgcttata tgcaaaaagc aggatgagca
    13501 gcagatacga aattttgtga acgatgtttt aatgaacaat aaggattttg cttaaaccaa
    13561 tacttgtttg catacgaaaa aggccgtagg gtaaacccac ggccttaagt attttagata
    13621 atatccctag tactcccaca aatcgtgctc aaagttgaag agctctgttt taatcttttc
    13681 ggactcaagt aatgtttgca aaccagtgaa agtgtactca tcaattgccc tgttatccca
    13741 tacgttcgat attctgataa tgtatgaatc aaaattacgt ttccagaaaa ggtcgtcgta
    13801 ggtaattcgc tgagcatcgt tgaagttgtt ataggcatcg gtattggcca atacacttct
    13861 ggcttcaggg aaataaatcc aaaacgttgg aattctgctt agggttcctt cttgttcctc
    13921 atcacttcct gttttacggt aatccgtacg atgaagaatg ggacaaattc caataatccg
    13981 aacctccata accgagcgct gcttatcaaa gaaccagtct tcctttacnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ttcagtttca gcaaaaactg taatttcaac
    14161 agtaggtgca cctgctcctg gttgggctat gtaacttccc attccaacag gcttgatgct
    14221 accacggcta atagtggccg acaccttatc gggggcaaat cctggaactg aaatatctat
    14281 ggggttttca acacccaggt aaagcacatt catcttggtt ggagcaataa ccacgttggg
    14341 tggcattact gtgtaggaat gcttgaatgg acggcgaaca attgttccat ctggtgcttt
    14401 catctcaatt aaaccttgcc agtttacaac accagttgat gaagctttac gggttaatac
    14461 ggctctacca gttttgttta ctggtacttc ctcagtgttt ccaataggac ggtaatcata
    14521 tccattgata gcgctattga aaacagagtc gtaacgacaa aggtaaactt tgggtacctg
    14581 agtggtatcg gaagcggcaa ggaaaatctc tgcacggaac tcgctaccct ggaaaataat
    14641 gttagagtta gggattactg tggctgaaag cttattaaat ttaaactctg ccgcaccaat
    14701 gctacccatc agatagttaa taatttcagc ttcaacgtta agaatatcaa cctgaacctt
    14761 tgtaagctgg ggtattacag caattaaagg aatatgctca aatcgtgcgc tctcccaggt
    14821 gttgtgtgtg ccatcttcct ttactggggg atcgtcagta ttaagaattt tcttgattga
    14881 ttcaacaagt tccggagcct tatccttgtc aattatgcta actatatact ccctgaattc
    14941 ttctaatttt tgttttaaga cctttgcctt gccattaccc tcaaacccta tcaggatggt
    15001 agcagctttg tcgaggtcgc ttttaccctg aatgagttca gcaataattt caccattctg
    15061 gattgctggg gtttcctcac cctccgatgc ttttaccacc tctattttaa ggtcctgaac
    15121 gtactgtaga atttcagtgg ctttcttttg aatttcgtcg gctttttgtt tatagggccc
    15181 aacctttaca ggatttatgc tgtaagcagc agcaaactca tcgtaaagtt tcttgttttt
    15241 aagccggtaa ctttctgtag ttcttgatag gccattgtca actaaaacaa aagcttcaag
    15301 aacctcagac gatacattaa gcgcaagcat agctgtcagc acgaggtaca tcatgccgat
    15361 catcttttgc ctgggagtct cttttccgtg tgccataaag ctatcttaag ctgtaatatg
    15421 caaaactgct actttccgtt tgatagggca ttgattgccg taagcatgtt gccgtaaaca
    15481 ttattgagag cagccacatt gctattcaac ttctcaactg ccaacctgaa cttctcggtt
    15541 tcttcaatag tgaagttaag tttcttaacc attccatcaa ccccctggta aaccttttcg
    15601 gcttccttaa gcctttggct ggtttcctgc agatgaagtt catgtgctgc attcagtgca
    15661 gccatattct tgttcaaccg ctcaagctgt tccttatagc ttgtacttcc gttggtaata
    15721 acatttccgt taagctgcat agcttcagct aactgctggt aggttgtagc aacctgcgac
    15781 gctgtttgct tgtaggtgtt tgcaagtcca tcggctgaga cttttagatt atcggcaacc
    15841 gattcggcag aaccaactat tcgttcgctt accttttgtc cagcattggt aagttcggcc
    15901 tccaggttct taactgattg gttaacacca tccatgaagt ttttgccact ttcggacatt
    15961 acacctgcag cctgctgtat gttttggttt aaaacattta ttgactcatt gagtgcttgt
    16021 ccgctctgag agtaattttg ggtaaaagta ccaacactct ccgatgcttt tgtcaatttc
    16081 tcgttgaact cctttacaac tgttgctgaa ttggtaatgt cggcaatttt gctcgatgca
    16141 tcacccagct tgttaagccc agcgctgacc ttatcgaaaa gggcaggact aatctctgcg
    16201 ttttcaagca tcttgttgaa cttctcggta aagatcaggg caccgggaac tcctacagca
    16261 gcaggttgtg aagccggtgc tgcggaagca ggagcggctg cagcaactcc agcagaccct
    16321 gcgggcaagg atgccagcac agaagtaata atttgttgaa catcctcagc agaaagacct
    16381 tggttcctgg agcggcggta tcctgaaatt tcctcttcat cggacattcc ggttaattca
    16441 ggatatacta gagtccagtc tacctcttcg tgaattggct caaaagcgga gaagaagaat
    16501 attattgatt ccgtacccat acctaggaaa agcatggtac cagcaccttt caggtgaaga
    16561 attttgaaaa gtgcaccaag aattaccact gaagcacccc atccgtaaag gtacttcatg
    16621 aaggttttcc atttcttgga tgttacaatt tcttctatgc tgatcttcat aactgtaatg
    16681 ttttcaatta gtaaaattca tcacttctac ttagctccga ggtatgaacg aacgcaacgg
    16741 aaaccaatat aagacttggc tgagtcctga tactcgtagg tacgtgaacc aacctgaagg
    16801 aagaagctaa tatctttcca cgaaccgcca cgaaccacct tacgctttag cgctggtaag
    16861 tcggatggtt ttgaattgta cttataatcg ggattcatat cgtgcataaa agagtatgca
    16921 ctttcatcgt aggcgttggc tgtccactca gctacgtttc cagccatatc gtatagtcca
    16981 taatcatttg gttcataagt gccaacgggg attggtatta aaccaccgtc gtcaacatag
    17041 ttaccacgga gaggtttgaa gttggccagg aagcatccct ccatgttacg ggtgtaaggg
    17101 ccgccccaag gatacatgct gtggttcaaa taaccacggg cagcatactc ccactcagct
    17161 tcggtaggta agcggtaatc ttgaatggta ataccttcag gggcaagaaa cttattgagt
    17221 gtttgggttc tccaaataca gaaggctgtt gcctgtttcc atgtaacgcc aacaacaggg
    17281 taattgtcga atgcaggatg ccagaagtaa ctattggtgt aaggttcatt gaacgcatag
    17341 gtaaaatcgg ctacccaaca aagagtatcg gggtaaacgt ttaccttatc gcgcatgata
    17401 aagctctggc ggttatcaac cttaaccttc tctcctaggc tgtttataac ctcgccctgg
    17461 tcgtaactga gggtttcgta gttatagcgg ttgcgttttt gagctgcttg cttaatatca
    17521 atccagtaat actcaaagaa tagtttacgg gtatcaacct ctttccgtcc aaagaattca
    17581 tcctccttgg aatagtatag ggcattgagg gcttcgcgtt gctcctcatc ttccatatca
    17641 attggaactt cccagttgag aacgggagga tccagagggt taccgaactg gtcctctgtg
    17701 attaggaact catcgtttat ttcaccaagt aggcgacgtg caatggagtc gcgaacatag
    17761 taaacaaact gccggtactg gttattagta atttcagttt gatccatcca gaaagcatca
    17821 accgaaaccg ttttagtggg agcattaatt gcccaggcaa catcctgatc attgctaccc
    17881 agagtaaatg aacccatggg aatgaacacc attcccagcg gtgttggctc gctgaaactt
    17941 ctacgtcctg gaacacccgt taactcgcca gtattaccag gtccacaacc ataaagtagg
    18001 gacgccaaaa tacctatgga gagaaatttt ttcataaggc atcgtttatt aagcaaataa
    18061 cgtaattttt ttcgttcaat tgtaaaaata tgttacaaaa atctcaaact tttatactgt
    18121 tggggtggct tttccttatt tagctggaag ttatacccca tgtaaaactc gtgagaaccg
    18181 ttgttgtact tactaatttt actggtacta aattcataag catacccaaa cttaatacca
    18241 ttaaaaagtt caaaaccaac caagccagta atcgcctcgc ccacacggta agaaacgcca
    18301 ccccatacct ttttattgta ccgaacaatt gtatttagcg ataatttact tgattttaaa
    18361 tcgctgtgaa caattactga aggctcaaac tgccatgtgg ggtttggaaa attaacaata
    18421 tatccaccag ttaggtagta ctgacgggta taccgtgcag gtaatgctgc cttattagtt
    18481 ttggtttcgt tcaggtgtag cgctgagaac ccaaaaaaca tgtattccgt actgtagtat
    18541 aaccctgcgc taaggtcaaa gttgattgaa ctttcctttt gatcaggaat tgcctcatcg
    18601 ccaccaccaa cggggtaatt ccattttggg tcgagcttat tatttacaaa cccagcacca
    18661 accccaaatc ctaaatctcc attaatcaat ttaaatttaa aacgggcagc ataggcaatg
    18721 ttaatgccaa ggtcattatt aaatccatag ctatcgttca ggatactcaa cccaactccg
    18781 ctcttgaatc caaagggcga tattggagca tttatgccta acgtattgct taagggtgca
    18841 ccatcgccaa aacctgccca ctggatgcgg ctgatggccc caacccctat catgttgttc
    18901 tgacttccag cgtatcctgg atttatagtt aacgggttaa agagtatttg attgaactga
    18961 gggtcttgct gtccgaaagc atgggacgac agcaaaacgc ttagtgtagt ggcaaataca
    19021 aggtttccaa atactctcat tggtagcttt taaaaattag tttgcctata tgaaccgaaa
    19081 agtaaagtaa gtaaaaattt taaattcata caaatttatt aacaaaatat gttaacaaaa
    19141 gtaacagtaa tgctttatac ctgcttttgc ctgtaggggt taccttttaa gcagattttt
    19201 ttttgaacgt tccaaaatat tggatttagt gttgaaaaat gttaaccaat gagttcctgg
    19261 ttaaattaga ttttttgagc tttttgatac caacgcaacc aacgttgagg gatctcgcca
    19321 ttacttgcct gtgaataatc cgatgcattg caccgtacta ataattttcc tcgcaaaagt
    19381 ttctttttac tatttacagg taactcaaac caccaattcc cggaaagttc acttctaaaa
    19441 aagatcattt caacattttg aacctcggta cttactacat actttttatt atggggtaag
    19501 tttttaatgg gattttcaac cctgcgatgg gaaacaccct caatatagtg ccaaagtacc
    19561 tgtgcggcaa gcataatggt ttgttcgggt gcattagttt ttaatccgca aattccaaga
    19621 aatttactgc tctgtccaat gccggcataa cgggaaagca tacaaatctc ctcagcatag
    19681 agtccattgg gattatcaaa tgcttttcca ggggcatcgg aagcgcgaat ggctgccata
    19741 tcaatgctaa ccagatttgc atcggacagt gtgggttctg cctcacggat atttccgcgc
    19801 aggttaccta acctgatata atcatgcttg cggcgcaaaa ccttttgtag ggtatgctgt
    19861 ggagtaaagt atccctgaat gccaataaaa cttaaatcga gcaatttctc gtagggctca
    19921 tcaattaaag tgttaacaaa gttatcctga tgaaaatcgt tgttgtttcc atcccaatca
    19981 attttactat caataatggc cattcggatg aagctctgga tttgcttcca accagcataa
    20041 acctttgata ggaattcctg tgagccacct aaaagaatga gatgcgcctt tgattcggca
    20101 ataattgccg aaattgactc cacaacttga tactgctggg ctaacgtaag agttgcagaa
    20161 atattaccag catcaaacac tttccccttt gagtaatgaa tggagctaag cccccaaagc
    20221 atttgacgta tattgtttgt taccgaatcc gaatctatcg attcaatacc tacaataaat
    20281 atatcacccg ggcgaatagg ggagtcgggt tggtcgtgta ggtttatcac ttggttgccg
    20341 aaacagtact ttttttctgc aaaagggtaa attcccgtta catcggaagg agcaaggtat
    20401 gctataagct catttgccag catggggtaa actatttctt tttagtagtt tttgtacctt
    20461 tcttacctgt agtttccttc ttttcggatt cttcaattat tttaagacag tcgttatagg
    20521 ttaattccgc tgcgttagtt cctttaggta ttttatagtt gcttttagct tttgaaatat
    20581 atggccccca cctaccatta agcaccataa gttcaggatc ttcagtaaaa gttttaatta
    20641 ccctttgttt ctctttcaat ctaccctctt caataatctc aatggctctg tttaaatcga
    20701 tagttaaagg gttatcatcc tttttaagtg agtaaaattt acccgcgtgc ctaacgtaag
    20761 ggccaaaccg gccaactgca actaccacct ctttaccctc aaagctacca agtgtacgcg
    20821 ggagtttaaa taaggacaaa gcctcctcaa gggttattgt tgaaaccaat tgccccttta
    20881 gcaagcttgc atactgaggt ttttccttac tgttggggtc gttgtcgcca agctgaacaa
    20941 cagggccata ccggccaatc cgaacgctaa cagtttttcc agttgccggg tcggttccta
    21001 aaaccctctc ccctttggtg taatccgact ccttttcggt ttttttaacg gtatcgtgga
    21061 acggcttata gaactcatct atcattttgg tccattccat cttccccatc gcaatctcat
    21121 cgaacttctc ctcaacctgg gcagtgaagt tatagttaac aatattggga aagtactcca
    21181 ccagaaaatc gttaaccaca attccaatat cgctggg
//
LOCUS       DAOOWJ010000038        21106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2512_length_21106_cov_7.516650, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000038 DAOOWJ010000000
VERSION     DAOOWJ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 21106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21106
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2512_length_21106_cov_7.516650"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..893)
                     /locus_tag="PLW22_05425"
     CDS             complement(171..893)
                     /locus_tag="PLW22_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU74180.1"
                     /translation="MRAMILAAGLGTRLLPLTADKPKALIEVNGKTLLEICINNLIGF
                     GFNHIVVNVHHHAQQIIDFLRNSSFNAQIDISDETERLLDTGGALVHARSFLDQGEPF
                     LVHNVDIISNIDLHYLYQYHLNSSALASLAVSKREASRVFLFNEQMVLSGWRNMVTGK
                     VIITNPSKALTAYAFSGIHVISPEIFKQLPPKGEFSIVDAYLKLSKEFLIQGADLSHS
                     DVIDVGKPDTLELASQFVTRKV"
     gene            complement(900..1025)
                     /locus_tag="PLW22_05430"
     CDS             complement(900..1025)
                     /locus_tag="PLW22_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74181.1"
                     /translation="MSKKKCAKGKAGVKVEGKDYTCIKCGASSNSKDKLCKPIKK"
     gene            complement(1030..2457)
                     /locus_tag="PLW22_05435"
     CDS             complement(1030..2457)
                     /locus_tag="PLW22_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOU74182.1"
                     /translation="MHSISHIENLSKLFSEVYGHSALNIVQLPGSGSARIYFRIESQQ
                     LSCIGTFNDDVKENETFYYLTEHLHHRNISVPKVLGVSSCRRYYLQSDLGSLSLYDKI
                     TDANSDTLEVQNLIGQAISNLARFNILGFDGIDESKLYPISHFDIKSVMWDLYYFKYC
                     FLKPSGVTYSEAELENVFGYLAEKVLNGDNQFLQFRDFQSRNIMILANQPYFIDYQSA
                     RVGSGLYDIASFLYQAKANFSDSTRQQFLVHYLDELSKYRSIDRSEMVAQFPYMAIFR
                     ILQTLGAYGFRGLFERKTHFIQSVPLALHNLSNLLAKVDDRRLRYLSQLNKELLSKYQ
                     VEQSEDFDGLTVQVYSFSIKSGYPPLNPEHGGGFVFDCRYLPNPGQLDLYKNLTGLDK
                     PVIDYLLSLDEVNVFCDNAFSLVKSAIDRYLSRNFKHLSVGFGCTGGQHRSVFCAENL
                     VKQLKSTFGDSGLRILVKHIELEKR"
     gene            complement(2450..3136)
                     /locus_tag="PLW22_05440"
     CDS             complement(2450..3136)
                     /locus_tag="PLW22_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOU74183.1"
                     /translation="MTGTLVNVIAVLAGGSIGLLIGSRMPEKYSKVFFQVVGLFTLVL
                     GLSMAIKTNEPLLVVFSLILGVLSGTLLDLQSRLDNLSNRLKNRFKISGNRFSEGLVV
                     AFLLYCMGSLTVLGAIEEGLGGTPKLFYLKSLMDGFSSIVLASTLGVGVLFSALPLLL
                     YQGSLTLIASFAASSLSGSMVNELSAVGGILLVTLGFNILEIKRFHVINMLPALIFIV
                     ILTSLFANHA"
     gene            3257..3739
                     /locus_tag="PLW22_05445"
     CDS             3257..3739
                     /locus_tag="PLW22_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74184.1"
                     /translation="MPLPTSSKMRISLLKKDEIIDFVEFNITGDAENYIRCDDESYYL
                     QTNVFNIFAGCFERSHHLFDYISPTMYNARRFIPLRNELLVNLEKLLVIKNVSQFQQY
                     LSQVFLGKDLLEVLKQIDPEYERHWKGYIRRLIQVNRHIMRLIDQCIDEERVLWVIPY
                     "
     gene            complement(3749..4837)
                     /locus_tag="PLW22_05450"
     CDS             complement(3749..4837)
                     /locus_tag="PLW22_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOU74185.1"
                     /translation="MKLVADSHIPYLKGVFEPFFDVEYLPGHLINREVVKDADVLIIR
                     TRTICNQDLLQGSRVKFIASATIGYDHIDTQYCDSNGIRWVTAPGCNAAAVQQWVGAA
                     LVKWISSKHLNPKTLTIGIVGIGNVGSKVVALGNALGMNVVCCDPPRAERENIKSFVD
                     LHELLKVSDIITFHVPLTRADNYPTSHMLSSKNLALCKPTAYFLNSSRGGVVDEVALL
                     SYLEQNPNVAVALDVWENEPDLNLELAKRCLVATPHIAGYSLEGKVMATKMVVDAVSR
                     YFNLGLTPWWPTPNPLTEKQVIGSAENILDLIGKTYRIERDDIRIIAKDFEDYRNTYN
                     YRRDFTGFKVLKIEEDAEKVGMLGFELG"
     gene            4931..5380
                     /locus_tag="PLW22_05455"
     CDS             4931..5380
                     /locus_tag="PLW22_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOU74186.1"
                     /translation="MIVAPLSEFGTYLNLHPLFERVEQALITLDFSKPGEKIYVDGEN
                     LIAIPSFNKARSAGEAPLESHDKYIDIQICLQGEETMGWRDRQTCSAIKEPYNSDNDI
                     TFYSDHPTCFITLKPNQFAIFFPSDCHAPLIGEGFIKKVVFKVKLAM"
     gene            5430..6725
                     /locus_tag="PLW22_05460"
     CDS             5430..6725
                     /locus_tag="PLW22_05460"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU74187.1"
                     /translation="MKRNLLRIAAVPAMLTMGACATILNTTKQKVNLTSNPIGAEVYI
                     NGKSTNKTTPCAVWVKRKVKKGPANGRNQYIYEFRKDGFVPYTYTDNAEVSSKIWLNL
                     FLNVAALPAFGVDFISGGAYYYNNDVLATLQKESAGYVVIRDTVVKQVYVTSPEAKGY
                     MFEKLSDVDVDIPNNNLEYPLRFALIIGNEDYSSKQVDLNREVNVDFARNDASAFKEY
                     AIKVLGIPESNIIFLLDATAGQMNQAIAKTNLIIKNAKGKAEVFVYYAGHGLPDEVTR
                     EPYLIPVDISGQNARDGIKLADLYAKLTEYPTKRVIVFLDACFSGGARNKELVAARGV
                     KVRPTESVLQGNIASLSAVSGEQSALPFKDKHHGFFTYYLLKKLKETKGNITFKELST
                     YLDEIIPLQSVLLNSKEQTPVLRMGINPTEDLENWVLTK"
     gene            6733..9114
                     /locus_tag="PLW22_05465"
     CDS             6733..9114
                     /locus_tag="PLW22_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU74188.1"
                     /translation="MALYLLKLGAKPSFFYSMIKKIIGILFITFLCFQGIKGQVKYNV
                     SGYVKDSQTGEVLIGATVIVKGINIGTATNSYGFFTITVPKGEHTISFGYLGYKTQEM
                     TLQVNKDNRIDIEISPNAQEIQEVVVSREKGNSNIQRAEMSLVKLDMKSVKQIPALMG
                     EVDVIKAIQLLPGVQSTAEGGSGFSVRGGASDQNLIILDEATVYNASHLMGFFSVFNN
                     DAIKDIKLYKGDMPASFGGRLSSVLDIKMKEGNSKDFNITGGIGSISSRLTVEGPILK
                     DRSSFMVSGRRTYVDVFFPLFNNDDLKQSTLYFYDLNAKLNLTLNPNNRIFISGYFGR
                     DMFGRDINGFGFGNQTLTARWNHIFKHNLFMNTTLISSNYTYFLESNTNDANAIRWES
                     NMDEVSAKIDFNYAIGPKYQLRFGLSSAYLTFMPGLIKGTNSQSFIQRWEIPKQHTLE
                     HGIYLSNEFDLGRISFKAGLRISAFQNIGKGQSLVLSPDYKVTDRISHPKGEIYNTYI
                     SPEPRVGLVYRLSDSSSIKVSYSHTTQYVQLASNSQGGNPLDVWFPASPNIKPQQADQ
                     WALGYFRNFSNNQIEASAEVYYKNVKNFVDFKDFADVLLNDELEADIRQGKAYSYGLE
                     LFTRIDLGKWEGWVSYTYGRTFRKTQGVNMGRIYSATYDKPHNATVVLSYRLNRQIIL
                     SANWIYSTGQTYTQPTGRYEIDNITIPIYSERNAKRYPDYHRLDFAVNIKTKKSLNRR
                     WKSEWNISVYNLYNRHNAWTINFVQDEKNPNITYAEKTYLFPIIPSVTYNFKF"
     gene            9117..9998
                     /locus_tag="PLW22_05470"
     CDS             9117..9998
                     /locus_tag="PLW22_05470"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOU74189.1"
                     /translation="MRKISTIIGILTLALAACTERIDIELDSSDSKLIVYGSITTDTM
                     SHYVRLIKSVPFYSPTPPPPVSNATVYIMEGNTRYDLTEDANNPGYYYTTPYVFGKSG
                     TTYTLHIEDVNIGGVTSFQAQTQMPTLLEGYQTNYLDSINVSYNRNWDGWVVNGYAKE
                     PQNMRNYYMFRVKINDILYSDSLSNLVLVDDKFFNGNNTEGAAFYFISEKDTLKVGDK
                     VTLELCAITQDYYQYLNEALKASSPQFPLFSPPPANPITNLNNGALGYFSAYAIIRAS
                     YTMKEEDFEGQKMKIYR"
     gene            complement(10078..13185)
                     /locus_tag="PLW22_05475"
     CDS             complement(10078..13185)
                     /locus_tag="PLW22_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU74190.1"
                     /translation="MSESLLDALIKLFAILVNIESKADSGFALDVLEDYLHHDFSDEQ
                     AKVFIDRFTNFGVSYRKELENVSLDRYTESPMVHAIIDSINHEFEQHQKVWLVLQLLE
                     FLADSRLTTPDRLEFARRVANQFNISDFEFSNGRDFILSESPGQIPWNQQVLLISPER
                     DNPIPEIKHLVNDRLQGQLYVLRIASTNTLLVKYFGELELFMNSRMLKPGRTYLFGPG
                     AVIRGPRMSPVYYSKVVSVFIQTPGKPFVSIIAKDIEYKHKGSRNGVYPFSFSAYSGQ
                     LVGIMGGSGVGKSTLINLLNGNLKPVSGKVYINGYDVHNSKTELEGVIGYVPQDDLLV
                     EELTVFQNLYYNALFCFSHLSKNEVVELVDKTLVDFDLVEARDLKVGNPLNKYISGGQ
                     RKRLNIAMELMREPSVLFVDEPTSGLSSLDAERIMLILRKQTFKGKLVITNIHQPSSD
                     VFKIFDKIIVLDQGGRPIFQGNPMDAVVYFKRMANFLKPEESECITCGNVNTDIILRI
                     VEARVVNEYGRLTRKRKRSAKEWYELYLQNIQSKIIVAEPACSSPIPPNNFKIPSKLQ
                     QMWIYFRRNLRSKLANSQFMNISILASPVLAVVISFFSKYIHGTLTNPNAYIFSENDN
                     IPAFIFMSVVAMIFLGLTISAEEIINDRKVLFREKFLNLSYFSYINSKLVVLSFFSAF
                     QSLLFVLISTYVLEIQGMFWSYWAILFSTSFCASMIGLNLSAALNSVVAIYVMIPLII
                     VPQLMFSGVVISYNKLHKSLSHSEYVPIIGDLTLSRWPYEALCVQQFSNNEYNKHFFF
                     INKDISNNNYNATLLIPKLVLLLQEAQTDLAVGKQTPRTRQRLSIVQNELERLKHNFP
                     IINFTYPDTGLLGISRVTPSYIDITLAKLDTLKSHFNSKYRVGVERRDALVKSLEKEL
                     VGKTTLFELSRMHHNNALEALVTNRNDFTKIVEVDNKLVRRYEPIYAYPTTRIGRAHF
                     YSYYKILGTLLVPTFWFNLVVIWLMSVIFYLTLLSDMFRVVNRFVERFRFRKLTARIA
                     RYLPT"
     gene            complement(13378..14850)
                     /locus_tag="PLW22_05480"
     CDS             complement(13378..14850)
                     /locus_tag="PLW22_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HOU74191.1"
                     /translation="MKAIQLFALTAFTYVVLSSCQKVGTIRVDSNAGISFAGKSYEVF
                     TTANDSVTRLALTSKGEFDSARQPFETEVAVFVNPSKQFQEIIGIGGAITDAAAETFA
                     RLTPENQQKLLEAYFHPVKGIGYSFIRTSIHSCDFSSGSYTYIADGDSTLATFSIAHD
                     MQYRIPLIKKALETSGSTTKLLASPWSPPAFMKDNNNMLYGGKLMQRYYQSWANYFVR
                     FVQEYEKAGIPVWGVTVQNEPMARQTWESCIFTAEEERDFLKHYLGPTFWNNGLKDKK
                     IIVWDHNRDLMPYRADVIFSDPEASKYAWGIGYHWYEDWWGGKKMYDNVRNVHESYPE
                     KHIIFTEGTVERFVPDHYNYWPNAEKYATSMIYDFNSGTSGWIDWNILLDEQGGPNHA
                     GNFCFAPMHGDTRTGELFITPAYYYLGHFSKFIRPGARRISTSPSRSNLLAASFNNTD
                     GSIVTVVLNTTDLPINYYLICNNAMAQLQIPAHAIQSIRY"
     gene            complement(15158..16345)
                     /locus_tag="PLW22_05485"
     CDS             complement(15158..16345)
                     /locus_tag="PLW22_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU74192.1"
                     /translation="MITRNKVSLVFVALAFAGITNAQMAFDRVLQQVDSNNLMLKALR
                     QKLEAERIATRIGLSPGNPQAEVIYQWGSPIDIGNKTTISITQQFDFPTAYIYRAKLA
                     KGISSALENEYRSKRTEILQKAGLLYADLVYHNALLVELNRQLKNALVIAQSLKQKFE
                     AGEIGVIPYTQAELVYHELNSRITQVKLEQKSLADELRALNGSVAIAIADSVIIVPEL
                     PDSFDSWFGLAEPQNPELAYLKSEIENSRLKLKLSKAEVLPTFNAGYASEKVGGERFQ
                     GIVAGISIPLWEKSNTVRYARANERYMQLLTDDYLLSFRQSSQRLYSKVKTLQEITNN
                     LSQNISSFDIAGKLYLALQKGEISIVDYYTELNSYNSIKERLLEVQHECLKTYIELTR
                     FAY"
     gene            complement(16329..19436)
                     /locus_tag="PLW22_05490"
     CDS             complement(16329..19436)
                     /locus_tag="PLW22_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU74193.1"
                     /translation="MLDRIIGFSLRNKYLILISTLVLTVIGLRTVSNMDIDVFPDLTA
                     PTVVVMTDAHGMAAEEVERLVTFPIETAVNGATDVRRVRSVSAQGFSFVWVEFDWGVD
                     VLKARQVVSEKLIAVAQQMPLGVSQPMLAPQSSVMGEIFFIGLQADSTSMMELRTIAE
                     WTVKPLILATGGVSQVTIIGGDYKQYQVVANPTRMLHLGVNMHELANACRFISMNSNG
                     VNIREFGNEYVVRGIARTSDIEQLGQTLVATRNGMPVKISDIAEIRIAPAPKMGYASS
                     NTSPAIILSISKQPNINTLEVTRKIEANLENLRKSLPPDVKLNTKIFRQSDFIERSVG
                     NVQQALIEGAIFVTIILLLFLGSFRTTIISLLAIPISLLATFVVMKLLGININTMSLG
                     GMAIAIGSLVDDAIIDVENVYKRLRQNREKPEHERQSSFTVVYEASREIRASILNATL
                     IIIVAFVPLFFLSGMEGRMLKPLGISFIVSLFMSLLVAMTLTPVLARMLLTGENYLKQ
                     NENEKWLAAKLRKGYEKSLQWVLRRKRLVLGSTLTLLVFSVILLFGMGRSFLPEFNEG
                     SLTLSVVTPPGTSLDETNKVGNYIEKMLLAVPEVTSTARRTGRGELDEHSQATNSSEI
                     DVNFQLKERSNDEFLADVRASLARIPGIAVTVGQPLGHRIDHMLTGTRASIAIKLFGD
                     DLTKLYTLANQIKTEIVGINGLVDVNVEQQVEVPQIQIRANRALLAEYGISLEEFNEF
                     VDIVLGGEKLADIYEGQQRFDLVVKLDSTYTSTIEGIKSILIDSPTHGKVPLYLVADV
                     ISTTGPNTISRENVQRKLVISANVAGRDLRGVVNDIRSRVDESITFPEGYRVEYGGQF
                     ESEARASRTLLIASLISILIIFMLLFVEFRNFSLAGIILLNLPLALIGGVFAIWLSSG
                     VLSIPAIIGFISLFGIATRNGILLISNYIRFRKMGFDVQEVVVKGSSDRLNAILMTAL
                     TAALALIPLAFKGHLSGNEIQSPMAKVILGGLLTSTFLNIYIIPIVYTMFDKNEIVSD
                     YDNQE"
     gene            complement(19449..20975)
                     /locus_tag="PLW22_05495"
     CDS             complement(19449..20975)
                     /locus_tag="PLW22_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU74194.1"
                     /translation="MVKNYLLIALATFIFTACNDTQRQQSQNHEGHSHEQAEQAEEEV
                     AHFTAYTDSLEIYIVGNPIIAGKEVELTAHVTGMKSFKPVKVDSLMLNVTAEGKSYSF
                     TALNTGNDGIYRLKVQFDVNGKADASIEMVINGIKGFYRLGQFTIYHCEHDWADSPEH
                     SHASNTIKFTKEQAWAVDFATEIVTPQPIGQVVKTTGMVMSAKADEVVLVSGISGVVS
                     YTEKSVLPGMQVRKSEVLLNLISKGMADDNFALRYAEAKSRYELAKSAYERQKALAAE
                     QVISTAEFQKAKSDYEVALAAFKAFENGYSAEGQQIVSPANAEVLKVFVSNGDYVNAG
                     QPLVKLSKLSVSSIQCYVQPRFLNLLSSISDANIRVSSSGMVVNLKSSGGRLAAVGRT
                     ISSENHLIPVVLELPNVEVLPVGEVVDVYLLCQPKGNSLTVPVSALLEEQGNHFVMVQ
                     LTPETFIKREVKLGVTDGSRVEVLSGLQPGNRIVIRGAVYVKMAQSSGALDAHSGHVH
                     "
BASE COUNT         6444 a         4602 c         4257 g         5803 t
ORIGIN      
        1 cgcgctcccc tccgaaagcg ggtgcaaaag tagaaaagcg tttttaatta cgcaaatcaa
       61 ttttcgaact tttttaagaa aaatgacaaa aattcaggca acgttctgtt agacagtaag
      121 aaaaatttta aaaaaatttc atttgaaaaa gacgcaggga gtattgcagg ttaaaccttc
      181 ctagttacaa attgcgatgc aagttccaga gtatcaggct tccccacgtc aattacatcg
      241 gaatgggata aatcggcgcc ctgaatcaaa aattcctttg atagtttaag ataagcatca
      301 acaattgaga actcaccctt tggtggcagt tgcttgaata tttcgggtga aataacatga
      361 atgccagaaa atgcatatgc agtaagggct ttggaagggt tagtaataat aaccttacca
      421 gtaaccatgt tcctccaacc agatagtacc atttgctcat tgaaaaggaa taccctggaa
      481 gcctctcttt tacttacggc tagcgaggca agcgcagatg agttaagatg atactgatat
      541 aggtaatgta aatcgatgtt tgagattata tcgacattgt gaaccagaaa aggctcgccc
      601 tgatcgagaa aactacgggc atgaacaaga gcaccaccgg tgtcgagtaa tctttcagtt
      661 tcatcggaaa tatcgatttg agcattaaaa ctgctattac gtaaaaaatc aataatctgc
      721 tgtgcgtggt gatgaacatt aaccactatg tgattaaaac cgaaaccaat taggttatta
      781 atgcatattt caagcagggt ttttccgtta acctcaataa gggctttggg tttatcggct
      841 gttaagggaa gtaatcgggt gcctaggcca gcggccagaa tcattgctct catataaact
      901 tactttttaa taggtttaca aagtttatcc ttgctatttg agctagcacc acactttatg
      961 caggtgtagt ccttaccctc aaccttaacc cctgctttac ctttcgcaca tttctttttt
     1021 gacattctgc taacgctttt caagttcgat atgcttaacc aaaattctta atccactatc
     1081 gccaaaagta cttttaagtt gcttaacaag gttctctgca caaaatactg atcgatgttg
     1141 gccaccagta caaccaaaac caaccgataa atgtttaaag tttcgagaca aatacctatc
     1201 tattgccgac ttaactaatg aaaaagcgtt atcgcaaaaa acatttactt catccaaaga
     1261 aagtaaatag tctattacag gcttatcgag tccggtcaga ttcttgtaca aatcgagttg
     1321 gccaggattg ggtaaatatc ggcaatcaaa tacaaaccct ccaccatgct caggatttag
     1381 tggtggatag ccactcttaa tagaaaaact atatacttga acggtaagac catcaaaatc
     1441 ttcactttgc tcaacttgat atttacttaa taactctttg ttaagctgac tgagatatct
     1501 taaacggcga tcgtcaacct tagcaagcaa attgctgagg ttatgcaacg caagaggaac
     1561 gctttgaatg aaatgggttt tacgctcaaa aagacctctg aaaccataag ccccaagtgt
     1621 ttgcaaaatt ctaaagatag ccatataagg aaattgggcc accatttcac tcctgtcaat
     1681 tgaacgatat ttggacagct catctaggta atgcactaga aactgctgcc gagtagaatc
     1741 gctgaaatta gcctttgcct ggtatagaaa agaagctata tcgtaaaggc ctgagccaac
     1801 tctggcgctc tgataatcaa tgaaatatgg ctggttagcc agaatcataa tattacggga
     1861 ttgaaaatcg cggaattgca ggaattgatt atcgccattg agcacctttt ctgctaaata
     1921 gccaaagaca ttttccaact cggcctcact ataagtaacc cctgaaggct taaggaagca
     1981 gtacttgaaa tagtacaaat cccacatcac cgatttgata tcgaaatgcg aaattgggta
     2041 aagctttgat tcatcaatac catcaaaacc caaaatattg aaccgggcaa ggttggatat
     2101 tgcttgccca atcaaattct gaacttcaag agtatcgctg ttagcatcag ttattttatc
     2161 gtacaacgac aatgaaccca aatcggattg taagtagtac cttctacaac ttgaaacacc
     2221 caataccttt ggaaccgaaa tattgcgatg gtgtagatgt tccgtgaggt agtaaaaggt
     2281 ttcgttttcc tttacatcat cgttaaatgt tccaatgcac gacagttgct gtgattcaat
     2341 cctaaagtag atgcgtgcag aaccagaacc gggcagctga acaatgttaa gggccgaatg
     2401 cccatatacc tcggaaaaga gcttggaaag attctcgata tgggaaatgc tatgcatggt
     2461 ttgcgaaaag tgaagttaga ataacgatga atattagtgc agggagcata ttgatcacgt
     2521 ggaaacgttt tatctctagt atattaaacc ctaaagtcac taaaagaata ccgcctacag
     2581 ccgatagttc atttaccatg cttccactta ggctgctagc cgcaaaagag gcgatcaatg
     2641 tcaatgaacc ctgatatagc agcaagggta aggcggaaaa cagaacccct accccaaggg
     2701 ttgatgctag cacaatggat gagaacccat ccataaggct tttgaggtaa aaaagtttgg
     2761 gtgttccacc caaaccctcc tcaatggcac ccagaacagt taaagaaccc atgcagtaaa
     2821 gcaggaaagc cacaaccagt ccttccgaaa agcgattccc tgatatttta aatcgatttt
     2881 ttaaccggtt cgataggtta tcgagcctgc tttgcaaatc gagtaaagtc cccgatagca
     2941 cacccagaat taagctgaat acaaccagta agggttcatt ggttttaatt gccattgata
     3001 agcccagtac cagcgtaaag agcccaacca cttgaaaaaa aactttactg tacttttcgg
     3061 gcatacggga tccaataagc aaacctattg agcccccagc caatacagct attacgttaa
     3121 ctaaagtccc agtcaccttt tagcattttt aatatactgc taaaatagaa tgtttacgtc
     3181 agaataaaca attgttaaaa aaaaatttaa gagtaaatga ataaagttag tatatttgaa
     3241 ttgaaaaata tgatttatgc ccttacccac tagtagtaaa atgcgtatta gcctgttgaa
     3301 aaaggacgaa atcattgatt tcgtggagtt taatattaca ggcgacgctg aaaactatat
     3361 ccgttgcgat gatgagtcgt attaccttca aacaaacgtg ttcaatatct ttgcaggatg
     3421 ctttgaacgg agccatcatt tattcgatta catctcgcca accatgtata acgcccgacg
     3481 tttcattcct ctccgtaatg aacttcttgt aaaccttgaa aaacttttgg ttattaagaa
     3541 tgtatcgcaa tttcagcagt acttaagcca agttttttta gggaaggatt tgctggaggt
     3601 tttaaaacaa attgatcctg aatacgaaag gcactggaag ggatatataa gaaggctcat
     3661 tcaggttaac cggcacatca tgaggctcat tgatcaatgc attgacgaag agcgtgttct
     3721 gtgggtaatt ccttactaag ccgattggtt aacccaactc aaacccaagc ataccaacct
     3781 tttcagcatc ttcttcaatc tttaatactt tgaaaccggt aaaatcgcgc cggtagttat
     3841 atgtattcct gtaatcctca aaatctttag ctataattct aatatcatct ctctcgatac
     3901 gatacgtttt gcctattaaa tctaaaatat tttcggctga gccgataact tgcttttcag
     3961 taagtggatt tggcgtaggc caccaagggg ttaggccaag gttaaagtaa cggctaacag
     4021 catcaactac cattttagtt gccataacct taccctccag cgagtaacca gcaatatgcg
     4081 gtgttgccac aaggcatcgc ttagcaagtt ccaaattcaa atcaggttca ttctcccaca
     4141 catctaaagc aacagctaca ttagggtttt gctccaaata gctaagcagc gccacctcgt
     4201 caactacccc accccgcgat gaatttaaaa agtatgctgt tggcttgcac aaagccaaat
     4261 tcttactgct taacatatgg ctggtagggt aattatctgc tcgcgttaaa ggaacatgaa
     4321 aggtgataat atcggacacc tttagtaatt catgtagatc aacaaaagac tttatattct
     4381 ccctctcagc cctaggcgga tcgcagcaaa ccacattcat gcctaaggca ttgccaagag
     4441 caaccacttt actgcccaca tttccaatgc ctactatacc aatggttagg gttttgggat
     4501 tgagatgttt tgaactgatc catttcacaa gtgcagcacc cacccactgc tggacagcag
     4561 cagcgttgca accgggagcg gttacccacc taataccatt tgaatcacag tactgagtat
     4621 cgatatgatc atagccaata gttgccgaag caatgaactt aacccttgaa ccttgcagta
     4681 aatcttggtt acagatagtg cgtgttcgaa taattagcac atcggcatct tttaccacct
     4741 ctcggtttat aagatgcccg ggtaaatact caacatcaaa aaagggttca aaaacacctt
     4801 taaggtaggg gatgtgacta tcggcaacta acttcatagg taaattttgc acaaagatag
     4861 caagtaaaaa ctgttatacc cagcattgtc aaactgtcaa aataaactaa ttttacaaaa
     4921 aatatgcgta atgattgtag caccattatc ggaatttggt acctacctaa accttcatcc
     4981 cctttttgag cgggttgaac aggcgctaat tactttggat ttcagcaaac caggagaaaa
     5041 aatttacgtc gacggggaaa acctcatagc catcccctcg tttaataaag cacgcagtgc
     5101 aggagaagcc ccattagaat cgcacgataa gtatatcgat attcaaatat gcctgcaggg
     5161 cgaagaaaca atgggctggc gcgacaggca aacctgtagt gctattaagg agccctataa
     5221 tagcgataac gacataacct tttactccga ccaccctaca tgttttatca ctctaaaacc
     5281 taatcaattt gcaatatttt tcccatccga ttgccatgca ccgttgatag gagagggttt
     5341 tataaaaaaa gtggtgttta aggttaaatt ggccatgtaa ttgcataaaa tttgtagtaa
     5401 aaatattacc aaaccaaact gaccaatcta tgaaacgcaa tttattgaga attgcagctg
     5461 taccggcaat gctaaccatg ggtgcctgtg caaccatact taacacaact aagcaaaagg
     5521 taaacttaac ctcaaatcca attggagccg aagtttatat taatggtaaa agcaccaata
     5581 aaactacacc ctgtgcggtt tgggttaagc gcaaggtaaa gaaagggcct gcaaacgggc
     5641 gcaaccagta tatttacgag ttccgtaagg atgggtttgt tccttacacc tataccgata
     5701 acgcagaggt tagcagtaaa atatggctaa acctgtttct aaacgtagca gccctacctg
     5761 cttttggcgt cgattttatc tcaggtggtg catactacta caataacgat gttttggcaa
     5821 ccctgcaaaa ggagtccgcc ggttatgtgg taatcaggga cacagttgtt aaacaggttt
     5881 acgtaacttc ccctgaagcc aagggttata tgtttgaaaa attatcggat gttgatgttg
     5941 atatcccaaa caataacctg gagtatccct tgcgctttgc gctgataatt ggaaacgagg
     6001 actacagtag caaacaggtt gatttgaata gagaggttaa tgttgatttt gcccgaaacg
     6061 atgccagcgc atttaaggaa tatgccataa aggtattagg gattcccgaa agcaacataa
     6121 tctttctgct cgatgccact gctgggcaaa tgaatcaggc cattgcaaaa acaaatctga
     6181 taataaaaaa tgccaagggc aaagccgagg tttttgttta ctatgcaggg catggcttac
     6241 ccgatgaggt tacccgcgaa ccatacctta taccggttga cattagcgga caaaatgccc
     6301 gcgatggaat taaactagcc gacttatatg ccaagcttac agaatacccc acaaaaaggg
     6361 tgatcgtatt tctggatgca tgcttctcgg gcggagcccg caataaggag ttagttgcag
     6421 cccgcggggt taaagttcgc cccactgaaa gcgtgcttca gggcaatatt gcttcactat
     6481 cggccgtatc gggcgaacaa agcgctctgc cattcaagga taagcaccat ggtttcttta
     6541 cctattattt actcaaaaaa ctaaaagaga ctaagggcaa cattaccttt aaggaactta
     6601 gcacctacct cgatgagata atacccctac agtcagtgct actgaacagt aaggagcaaa
     6661 cccctgtgct tagaatgggt attaacccaa ccgaggattt agaaaattgg gttcttacaa
     6721 aatagaacta atatggcatt gtatttgtta aagctaggtg ctaaacctag ctttttttat
     6781 tccatgatta aaaagattat tggcatactt tttattacat tcctttgctt ccaaggcata
     6841 aagggacagg taaagtataa cgtaagcggt tacgttaagg attcccaaac gggcgaggtt
     6901 ctcattggag caacagtaat agtaaaggga atcaatatag gtactgcaac aaatagctac
     6961 gggttcttta caattacagt tccaaaaggt gagcatacaa tctcatttgg atatctggga
     7021 tataaaaccc aagaaatgac tttacaagtc aataaggata ataggattga tattgagata
     7081 tcgcccaacg cacaggaaat ccaggaggtg gttgttagcc gggagaaagg gaatagcaat
     7141 attcaacgag ccgaaatgag ccttgtaaag cttgacatga aaagtgtaaa gcaaatacct
     7201 gccttaatgg gtgaggttga tgtaattaaa gctattcaat tactgccggg ggtccaaagt
     7261 actgccgaag ggggatcagg atttagcgtt cggggtggtg cttcggatca gaacctgata
     7321 atactcgatg aggcaaccgt ttacaatgct tcgcatctaa tggggttttt ctccgttttc
     7381 aataacgacg caattaagga tatcaagctc tataagggcg acatgccagc ctcgtttgga
     7441 ggcagattat cgtcagtgct cgatataaag atgaaggaag gcaacagcaa ggattttaac
     7501 ataaccggag ggataggtag catatcgagc cggttaacgg ttgaaggtcc aatactaaag
     7561 gataggagtt cgttcatggt atcgggacgc agaacctatg ttgatgtgtt ttttccacta
     7621 tttaataatg acgatttgaa acaaagcaca ctctactttt acgacctgaa cgcaaaactg
     7681 aacttaactc ttaatcccaa caaccgaatt tttatatccg gatactttgg acgcgacatg
     7741 tttggccgcg atatcaacgg ctttggcttt ggtaaccaaa cgcttacggc aaggtggaac
     7801 catattttca agcataacct tttcatgaat actactttga tatcaagtaa ttacacatac
     7861 tttttggaat cgaataccaa cgatgcgaac gccattaggt gggaatcgaa catggatgag
     7921 gttagcgcaa aaattgattt taactacgca attggtccaa aatatcagct taggtttggc
     7981 ctatcgagcg catacctaac atttatgcct ggtttgataa aaggtactaa ctcccaatcg
     8041 tttattcagc gttgggaaat tcccaaacag catacgctgg agcatggtat atacttatcg
     8101 aatgagtttg acctaggaag aatttcattt aaagcaggtt tacgcatatc ggcctttcag
     8161 aacattggga aaggccaaag ccttgtcctt tcgcccgact acaaggttac cgacagaata
     8221 tcccatccaa agggtgagat ttataataca tatattagtc ctgagccccg agtgggctta
     8281 gtttacaggc ttagcgatag cagctccata aaagtcagct actcgcatac cacgcagtat
     8341 gttcagctgg catcaaactc acaaggtgga aatccgcttg atgtttggtt tccggcatcg
     8401 cccaatatta agcctcagca agctgaccag tgggcccttg gctactttag gaacttttcg
     8461 aacaaccaaa tagaggcatc ggccgaggtt tactataaaa acgtaaaaaa ttttgtggac
     8521 tttaaggatt ttgccgatgt tctgctcaac gatgagcttg aagctgacat caggcagggc
     8581 aaagcatact cttacggatt agaattattc acacgtatcg acctagggaa gtgggaagga
     8641 tgggttagct atacttacgg cagaaccttt cgcaaaacac agggagttaa tatgggaagg
     8701 atttatagcg caacctacga taagccccat aatgcaaccg ttgtgcttag ctatcgactt
     8761 aataggcaaa ttatcctttc agccaactgg atttactcca cgggtcaaac ctacacccag
     8821 ccaaccggac gatacgaaat tgataatatt accataccca tatactctga aaggaatgcc
     8881 aaacgctatc cggattacca caggctcgac tttgccgtaa acattaaaac taagaaaagt
     8941 cttaaccggc gatggaaaag cgaatggaat atctctgttt acaaccttta caacagacat
     9001 aatgcatgga ccataaattt tgttcaggac gaaaagaatc cgaacataac ctatgccgaa
     9061 aaaacatacc tattcccaat tataccctca gtaacctata acttcaagtt ttagctatga
     9121 gaaaaatatc gaccattata gggattttga ccttagcact agctgcatgc acggagcgga
     9181 tagacattga actagattca tcggattcca agcttatagt ttacggcagc ataaccaccg
     9241 ataccatgag ccactacgta aggctaatta aatcggtacc attctatagt cctactccac
     9301 ccccacccgt ttccaatgcc acggtttaca tcatggaagg caatacccgc tacgacctca
     9361 ccgaggatgc caataacccc ggctactact acacaacccc atatgttttt ggtaaatcgg
     9421 ggacaacata cacgcttcat attgaagatg tgaatattgg gggtgttact tcgtttcagg
     9481 ctcaaacaca aatgccaaca ctgctcgagg gatatcaaac caattacctg gattcaataa
     9541 acgtaagcta caacaggaac tgggatggat gggtagttaa tggttatgct aaagagcccc
     9601 aaaacatgag aaactattac atgttccggg taaaaataaa cgacatacta tacagcgaca
     9661 gcctcagtaa tctggttctg gttgatgata agttctttaa cggcaataac actgagggtg
     9721 ctgccttcta ctttatttct gagaaagata ctctcaaagt aggcgataaa gtaaccctag
     9781 agctttgcgc cataacccag gattactacc agtacctaaa cgaagccctg aaggcctcaa
     9841 gcccacaatt cccattattt agccctcctc cagccaatcc gattactaat ctaaataatg
     9901 gagcgttggg atattttagc gcttacgcaa ttattcgggc ttcgtacacc atgaaagaag
     9961 aggattttga gggacaaaaa atgaagattt acagataata tttgctccta aatattagaa
    10021 accacttcaa gaaacagctc aagactcaat taaataccag cattgcgaaa aacaatgtta
    10081 ggttggcaaa taccttgcaa tacgagccgt aagcttgcgg aaacggaagc gctcaacaaa
    10141 acggttaacc acgcggaaca tgtcgcttag taatgttagg tagaatatta ctgacattag
    10201 ccagataacc accagattaa accagaatgt agggactagc aaagtgccta gaattttata
    10261 gtaggaataa aaatgtgctc ttccaattcg ggtagttggg tatgcgtata tgggttcgta
    10321 gcggcgaaca agcttgttat ctacttcaac aatcttggta aaatcgtttc tattagttac
    10381 caaagcctcc aaagcattat tgtgatgcat gcggcttaac tcaaaaagtg tggttttacc
    10441 aaccaattcc ttttcaagcg attttaccag cgcatcgcgc cgttcaaccc caactctata
    10501 ctttgagttg aagtgagatt ttagggtatc gagcttagca agtgttatgt cgatatagct
    10561 aggcgtaact ctactaatgc ccaggagtcc tgtgtcaggg taagtaaaat taataattgg
    10621 gaaattgtgc tttaaccttt cgagttcatt ctgcacaatg cttaacctct gcctggttcg
    10681 aggcgtttgc ttgccaacag ccaaatcggt ttgtgcttct tgcagcaata acaccagctt
    10741 aggaatcagc aacgtggcat tgtagttgtt attgctgata tctttattga taaaaaagaa
    10801 atgtttatta tactcattgt tactaaactg ctgtacacaa agagcctcgt agggccaacg
    10861 gctcagcgtt aaatctccaa taattggaac atactccgaa tgggaaaggc tcttgtgtaa
    10921 cttgttatag ctgattacca caccgctgaa catcagctgc ggaacaatga ttaagggaat
    10981 cattacgtaa attgctacaa ccgagtttaa tgcggctgaa aggttcagac ctatcatgct
    11041 tgcacaaaat gatgtggaga acagaatggc ccagtaggac cagaacattc cttgaatctc
    11101 aagcacatag gttgatatta gaacaaatag taacgattga aatgctgaaa aaaatgaaag
    11161 tactacaagt tttgagttga tatagctgaa atagctgagg ttaagaaact tttcccggaa
    11221 aagaactttt cggtcgttaa ttatctcctc agcactaata gtcagaccta aaaaaatcat
    11281 ggccaccacg ctcatgaaaa tgaacgcggg aatattatcg ttttcgctaa aaatgtaagc
    11341 atttgggtta gtaagggttc catgaatata cttggagaag aaactgataa caaccgctaa
    11401 tactggcgaa gccagaatgc taatgttcat gaactgggag ttagcaagct tacttcgtaa
    11461 atttctccta aagtaaatcc acatctgctg tagtttcgat gggattttaa agttattggg
    11521 cggtattggc gaagagcagg ctggttcagc aacaataatc ttactttgga tattttgtag
    11581 gtaaagttca taccattcct tagctgaacg ttttcgcttt cgggttaagc ggccatactc
    11641 gttaacaacc cgggcttcaa caatcctaag aataatgtcg gtattaacat ttccacaggt
    11701 aatacactca ctctcctcgg gcttaagaaa attggccata cgcttgaaat aaaccacggc
    11761 atccattgga ttaccctgga atattggcct ccccccctgg tcaagcacaa ttatcttatc
    11821 gaatatctta aagacatcgg atgatggctg atggatattg gtaattacaa gtttcccctt
    11881 aaatgtttgc tttcgtaaaa ttagcattat gcgctcggca tcgagcgatg agagccctga
    11941 ggtaggctca tcgacaaata gaaccgatgg ctcacgcatt agctccatgg caatgttaag
    12001 gcgcttgcgc tgccctccgc taatgtactt attgagcggg ttgccaacct taaggtcgcg
    12061 tgcctcaacc aggtcgaaat ctacgagggt tttatctacc agctctacaa cttcattttt
    12121 actgagatgg ctgaaacaga aaagggcgtt gtagtagaga ttttgaaaga ccgtaagctc
    12181 ttcaaccagt aaatcgtcct ggggaacata gcctataacc ccttcaagtt cggttttgct
    12241 attatgaaca tcgtacccgt tgatataaac cttacctgaa acgggcttta aattaccatt
    12301 gagcaggttg ataagggttg acttgcccac gccacttccg cccataattc ccaccagctg
    12361 accggagtag gctgagaatg aaaaggggta aactccattc cgactcccct tgtgcttata
    12421 ctcaatatcc ttagctataa tggaaacgaa aggcttaccg ggggtttgaa taaaaaccga
    12481 tacaaccttg ctgtagtata ctggcgacat acgtggccca cgaattaccg caccaggacc
    12541 gaaaaggtat gttctccccg gctttagcat tcggctattc atgaaaagtt ccagctcacc
    12601 aaagtacttc accagcaagg tgttggtgct ggcaatccta agtacgtaaa gttgcccctg
    12661 aagtcggtcg tttaccagat gtttaatttc cggtataggg ttatcgcgtt cagggcttat
    12721 gagaagaacc tgttggttcc atggtatttg gccgggcgac tcgcttagta taaaatcgcg
    12781 gccgttggaa aactcaaaat cactaatatt gaactggttg gctaccctac gggcaaactc
    12841 aagcctatcg ggtgtggtaa gcctgctatc ggccaggaat tccaacaact gtaaaaccag
    12901 ccaaaccttt tgatgctgct caaactcatg gttgatagaa tcaattatgg catgaaccat
    12961 gggcgactcg gtgtagcggt caaggcttac attctctaac tccttacggt agcttacccc
    13021 aaagttggta aatctatcaa taaacacctt agcctgctca tcgctaaagt cgtgatgcag
    13081 ataatcttcc aagacatcga gagcaaaacc actgtcggct ttactctcaa tgttcacgag
    13141 tatggcaaaa agctttataa gtgcatcgag cagcgactcg ctcatggttt aaggtttgga
    13201 taagcaaaga tagaattttt caattgtatt gaaccgtccg cttataaatt ggattgtaat
    13261 tttataatcg cattacccaa aaattgatga caaccacctt tacctagcga gtgcttttaa
    13321 taaccattaa accatcgtaa aattaattca atactcaacg ctttactctt ccttaattta
    13381 atacctgata ctttgaattg cgtgtgctgg tatttgcaat tgagccatag cgttattgca
    13441 aatcagatag tagtttatgg gtaaatcggt ggtgtttagt acaaccgtta ctatacttcc
    13501 gtcggtatta ttgaacgatg cggcaagtaa attgctgcgc gatggtgagg tgcttattcg
    13561 ccgtgctcca ggcctaataa acttggaaaa atggccaagg tagtagtatg caggggtaat
    13621 aaaaagttca cctgttcgtg tgtcgccatg catgggagca aagcaaaaat ttccagcatg
    13681 gttaggtcca ccctgctcgt cgagtaggat attccaatca atccatcctg atgtgccact
    13741 attaaagtcg taaatcattg atgtagcata cttttcggca tttggccagt aattgtaatg
    13801 gtcaggcaca aaacgttcaa ccgttccctc agtgaaaatg atatgttttt cggggtaact
    13861 ttcgtgaaca ttccttacgt tatcgtacat ctttttcccg ccccaccaat cctcatacca
    13921 gtgatagcca ataccccaag catatttcga tgcttcaggg tcagaaaata taacatcggc
    13981 acggtaaggc atcaaatcgc ggttgtggtc ccaaacaatt atctttttat cctttagtcc
    14041 attgttccaa aaggtggggc caaggtagtg cttcaggaaa tcgcgctcct cttcggcagt
    14101 aaatatgcac gactcccacg tttgacgagc cataggctca ttctgaacag taacacccca
    14161 aactgggatt ccggctttct cgtattcctg tacgaaccgg acaaaataat tagcccacga
    14221 ctggtagtaa cgctgcataa gcttaccacc gtaaagcata ttattgttgt ccttcatgaa
    14281 agccggtggg ctccagggac ttgcaaggag tttagtggta ctgcccgatg tttccagcgc
    14341 ttttttaatg agaggtatcc tgtactgcat gtcgtgcgct atgctaaagg tagccagtgt
    14401 gctatcgcca tcggcaatgt aagtgtagct accgctgctg aaatcgcagc tatgaatact
    14461 ggtgcgaata aatgagtaac ctatcccctt taccgggtga aagtaagctt cgagtagctt
    14521 ctgctgattc tcaggtgtta aacgggcaaa ggtttcggca gcagcatcgg taatggctcc
    14581 accaatacca attatctcct gaaactgttt acttggattt acaaaaacag ccacttcggt
    14641 ttcaaaaggt tgacgagccg aatcgaattc cccttttgaa gttagtgcta gtcgggtaac
    14701 cgaatcgttt gctgttgtga aaacctcata gctctttccg gcaaatgata tgcctgcatt
    14761 gctatcaacc ctaatcgttc ctaccttttg gcaagatgat aatacaacat aagtaaacgc
    14821 ggtaagggca aacaattgta tcgctttcat tccaattcct tttttgagtt cgtttaacaa
    14881 agatacatcg gtggagtgaa gacttattgg aatgattcac aataattttt aatgagcaag
    14941 atttgagaaa atattgcaaa aaaagatttg cttataccat caatacttac caatcattag
    15001 taagtttacc accttcagta ttaaatgtga tttgtgtaga tttgagaagc taatagatag
    15061 ttaactccta ctacatcatc ctcgggttaa tgaaaaaaac gcgcatcaaa taatgcgcgc
    15121 tctttaacta taaaaaaatc catgcaaata cgaaacatca ataggcaaac ctagtaagtt
    15181 ctatgtaagt tttaaggcac tcatgttgca cttcaagcaa tcgttccttg atactattgt
    15241 agctatttag ctctgtgtag taatcaacaa ttgatatttc acccttttga agcgcaaggt
    15301 ataacttacc agcaatatcg aaactactga tgttttggct aaggttattt gtgatttctt
    15361 gcaatgtttt aaccttactg taaagtcgtt gtgaactttg cctaaatgat aataggtaat
    15421 cgtcagtaag caattgcata tacctctcat tagcccttgc atacctcact gtatttgatt
    15481 tttcccataa tggaatggaa atacctgcaa caataccctg aaagcgttca cctccaacct
    15541 tttcgctggc ataaccagcg ttaaaagtag gcaaaacctc tgcctttgaa agtttaagtt
    15601 ttaaacgact attttcaatt tcgcttttta ggtaggcaag ctccggattt tgcggttcag
    15661 caagtccaaa ccaggaatca aaactatcgg gcaattccgg aacaataatt actgaatcag
    15721 caatagctat agcaaccgaa ccattcaaag ctctaagctc atcggctaag cttttctgct
    15781 caagttttac ttgagtgatt cttgaattta actcatggta aacaagctct gcctgtgtgt
    15841 atggaattac acctatttca ccagcctcaa acttttgctt aagcgattgg gcaataacca
    15901 aagcattttt cagctgccta tttagctcaa ctaaaagagc gttgtggtaa accaaatcgg
    15961 cataaagcaa accagctttt tgaagtatct cagtgcgctt tgacctatac tcattctcta
    16021 aagcacttga tatgcctttg gcaagcttag ccctgtaaat atatgcagtt ggaaaatcga
    16081 actgctgagt aatactgatt gtggttttgt ttccaatatc tataggacta ccccactggt
    16141 aaataacctc ggcttggggg ttaccgggcg ataatcctat acgggtagca atcctttcgg
    16201 cttcaagctt ttgccttagt gcttttaaca taaggttgtt agaatcaacc tgctgcaaaa
    16261 ccctatcaaa tgccatttgg gcatttgtaa tgccagcaaa agcaagcgca acaaaaacta
    16321 agctaacttt attcctggtt atcataatca cttacaattt cgtttttatc aaacatggtg
    16381 taaacaattg gtataatgta tatgttcaga aatgttgatg ttaaaagtcc acccagaata
    16441 accttagcca ttggactttg tatctcgttt ccggaaaggt gtcccttaaa ggcaagtggt
    16501 attaaagcaa gggcagctgt aagcgcagtc ataagaattg cgtttagccg atccgacgaa
    16561 cccttaacta ccacctcctg aacatcaaat cccatttttc taaaacgaat gtagttagat
    16621 attaacagta ttccatttcg tgtagcaata ccaaaaagcg agataaatcc gataattgcc
    16681 ggaatgctta aaactcccga agaaagccat atggcaaaaa ccccaccaat aagcgccaag
    16741 ggaaggttca atagtattat tccagccaag ctgaagttac gaaattccac gaaaaggagc
    16801 ataaaaatta ttaaaattga aatgagagaa gctataagga gtgtccgcga tgcacgggcc
    16861 tcgctctcga attgtccacc atactcaact ctatatcctt cagggaaagt gatactctca
    16921 tcaaccctgc tacgaatatc gttaacaaca cctcgcaggt cgcgccctgc tacgttggcc
    16981 gatataacca gcttacgttg aacattctca cgactaattg tgttggggcc ggtagttgaa
    17041 atcacatcgg caaccaggta aagaggaacc ttgccatgcg taggcgagtc tattagaatc
    17101 gatttaatcc cttcaatggt agatgtgtag gttgaatcaa gcttaaccac taaatcgaaa
    17161 cgttgctgcc cctcgtatat atcggcaagt ttttcgcccc caagcacaat atcaacaaac
    17221 tcattgaact cctcaaggct gatgccgtat tctgccagta gtgcccggtt ggctctaatc
    17281 tggatttgtg gcacctcaac ctgctgctca acgtttacat caaccaaacc gttaattccc
    17341 actatttcag tttttatctg gttggctagg gtgtaaagtt tagtcaggtc gtcgccaaag
    17401 agcttaatgg caatgcttgc ccgcgtacca gtaagcatat ggtcgatacg gtgccctaaa
    17461 ggctgcccaa cagtaactgc gatgcccgga atgcgggcaa gcgatgctcg aacatcggcg
    17521 aggaactcat cgttgctgcg ctccttaagc tgaaagttca cgtctatctc cgagctgttg
    17581 gtagcctgtg aatgctcatc gagctcgcct ctacctgttc ggcgggctgt tgatgttacc
    17641 tcgggtactg ccagaagcat tttttcgatg tagtttccca ccttattggt ttcatcgagc
    17701 gaggtgcccg gtggtgttac caccgatagc gttagcgaac cctcattaaa ctccggaaga
    17761 aaactacgcc ccattccaaa taagagtata accgaaaaaa caagcagtgt cagggtgcta
    17821 cctaacacta atctttttct tctaagaacc cattgcaacg acttttcata gcctttcctg
    17881 agcttagcag ccaaccattt ctcgttctcg ttttgtttga ggtagttctc gcccgttagc
    17941 aacatgcgcg ccagcacagg ggtaagagtc atagcaacca gcaacgacat aaaaagcgat
    18001 acaatgaatg aaataccaag tggtttaagc attcgccctt ccatgccgct caagaaaaac
    18061 aagggaacaa aggctacaat aataatcaaa gttgcattca ggattgaagc cctaatctca
    18121 cgcgaagctt cataaacaac cgtaaagctc gattgccttt catgctcagg tttttctcta
    18181 ttttgcctca atcgcttgta aacattttca acatcaatta tggcatcgtc aaccagcgaa
    18241 ccaatggcaa ttgccattcc gccaagactc attgtattta tgttgatacc gagcaatttc
    18301 atcaccacaa aggtggcaag taacgaaatg ggtatggcaa gcagcgatat tattgttgtc
    18361 cgaaaactac ccagaaaaag tagaagaatt atggttacaa agattgcacc ctctattaat
    18421 gcctgctgaa cgttacccac tgagcgctca ataaaatcgg attgacggaa tattttggtg
    18481 ttaagtttga catcgggggg taacgatttt ctcagatttt ccagattagc ctctatttta
    18541 cgagttacct caagcgtatt aatattaggt tgctttgaga ttgatagaat aattgccggt
    18601 gaggtattgc tagatgcata acccatttta ggagcaggtg caatacggat ttcagctatg
    18661 tcgctaatct ttacaggcat tccgttacgg gtagcaacca gtgtttgacc aagttgctca
    18721 atatcggaag tgcgcgctat tcctcggact acatactcgt tgccaaactc ccttatgtta
    18781 accccattgg agttcatgct gataaatcgg catgcgttgg ccaactcatg catattaacg
    18841 cccaaatgaa gcatgcgtgt gggatttgcc accacctggt actgcttgta atcgccacca
    18901 ataatggtaa cctgcgaaac tccaccagtg gcaagaataa gcggcttaac cgtccattct
    18961 gcaatagtcc gaagttccat catacttgta ctatcggcct gcaggccaat gaaaaagatt
    19021 tcgcccatca cactgctctg tggtgcaagc ataggttggc ttacgcctaa gggcatttgt
    19081 tgggcaaccg caataagctt ttcgctaact acctggcgtg cctttaaaac atctacaccc
    19141 caatcgaact ctacccaaac aaacgagaaa ccctgagccg atactgacct aacccgccta
    19201 acatcggtag cgccattcac agcggtctca attggaaatg ttactaacct ctcaacctcc
    19261 tccgctgcca tgccgtgagc atcggtcatt acaacaacgg taggcgctgt aaggtcagga
    19321 aaaacgtcaa tgtccatatt ggatacagtt cggagtccaa taaccgtaag cactagggtg
    19381 cttataagta tgagatattt atttctcagc gagaaaccga ttattctgtc gagcatagcc
    19441 agttagtttt agtgaacatg acccgagtga gcatcgagcg cacccgaact ttgtgccatc
    19501 tttacataca cggcaccccg aattacaatc cgattgccag gttgcaagcc cgataaaacc
    19561 tcaacccttg aaccatcggt aacacctagc ttcacctcgc gtttaatgaa agtttccggg
    19621 gttagctgca ccatcacaaa atggttaccc tgctcctcca ggagcgctga aaccggaaca
    19681 gtaagggagt tgcccttggg ttggcaaagc aggtaaacat caaccacttc gcctactggt
    19741 aatacctcaa cattgggtaa ttccagaaca accggaatta aatggttttc cgatgatatt
    19801 gttctgccta ctgcagctaa cctgcctcct gatgatttaa ggtttacgac cattccggaa
    19861 gagcttaccc taatatttgc atcggaaatt gagcttagta aattcaggaa acgaggctga
    19921 acgtaacact gtatacttga aacagatagt ttactcagct ttactaaagg ctgacctgcg
    19981 tttacgtaat cgccatttga tacaaacacc ttcagaacct cagcatttgc agggcttaca
    20041 atttgttgac cctcggctga atatccgttt tcaaaggcct taaacgcagc aagggctacc
    20101 tcgtagtcgc tttttgcctt ttggaactca gcagtggaaa taacctgctc agctgccaaa
    20161 gctttttgac gctcgtaagc ggacttggca agctcatacc tacttttagc ctcggcatag
    20221 cgtaatgcaa agttatcgtc ggccatgccc ttggaaatta agttaagcaa aacctccgac
    20281 ttacgaacct gcattccggg taaaacagat ttttcggtat agctcaccac tcccgaaata
    20341 cctgacacca aaaccacctc gtcagccttt gccgacataa ccatgccggt agtttttaca
    20401 acctgcccaa ttggctgtgg agtaactatt tcagtagcaa aatcaacagc ccaagcctgc
    20461 tcctttgtaa atttgatagt gtttgatgca tggctatgtt cgggcgagtc tgcccaatcg
    20521 tgttcgcaat gataaatggt aaactgcccc aaccggtaaa atcctttaat gccatttatt
    20581 accatttcaa ttgaagcatc ggccttaccg tttacatcaa attgtacttt tagcctgtaa
    20641 attccatcgt taccagtatt taaagctgta aaggaatacg attttccttc ggctgttacg
    20701 ttaagcatta atgaatcaac ctttacaggt ttgaagctct tcatccctgt tacgtgagcg
    20761 gtaagctcaa cctccttgcc ggcgataatt gggttgccaa caatgtatat ctcaaggcta
    20821 tcagtataag ctgtaaagtg cgcaacttcc tcttcggctt gttcagcttg ttcatgcgaa
    20881 tgcccttcat ggttttgact ttgttgcctt tgagtatcat tacaagcagt aaaaataaag
    20941 gtagcaaggg ctattagtaa ataattttta accattccaa atcgaattaa ttattaacga
    21001 aaaatgaata gtaaacttta gttagtgata ctaaccatga atacaaacca actaaatcga
    21061 tttagaaagg gggagccctt aatgtgtggc agctaataat tggaat
//
LOCUS       DAOOWJ010000039        21088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2515_length_21088_cov_7.938050, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000039 DAOOWJ010000000
VERSION     DAOOWJ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 21088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21088
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2515_length_21088_cov_7.938050"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..735
                     /locus_tag="PLW22_05500"
     CDS             193..735
                     /locus_tag="PLW22_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOU74195.1"
                     /translation="MKILGMIILLATTLSTTNAQNVSFHSFVVTDIDGNEFPLSKLKG
                     KKVMVVNTASKCGFTPQYEDLQALYDKYKDKNFVIIGFPANNFMGQEPGTNQEIKEFC
                     TTRFSVSFPMMAKISVKGNDTHPLYQWLTQKSMNGVMDSEVKWNFQKYLIDENGKLVD
                     MIPPKEKPNSDKVIKWIEGN"
     gene            complement(751..2238)
                     /locus_tag="PLW22_05505"
     CDS             complement(751..2238)
                     /locus_tag="PLW22_05505"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU74196.1"
                     /translation="MWKYLVFLVGILVTHDLAISQTKIRQSPIDSKDLMNTVKFLSSE
                     RFKGRLPGSVEYEQASRYVATRFKNAGLNPLLGNTMLQEFDEEVNYILDARVFLLDQN
                     NRPTQPMYLGEDFVCRGFTGSGEVRGETVFAGFGIKNDEYNDYRSIDVRNKIVIVFKS
                     TPPWQASSGSWGDVSPRGKARIAKELGAKAIIFIGEPHMTPNTMVYGSIACGPKPHLA
                     DFPMIHAGSRFTDSLFSKLPYKPADFYEMIKRDKAPHSVETDKSIYINVRAVYQEAKK
                     TFNVVGYIEGSDPKLKHEYIILGAHLDHVGYQGDKLYFPGANDNASGVAGLIAIAEAI
                     NKSTTKPLRSVVFVAFSSEESGLKGSKYFVANMPINADKVVAMLNFDCIGQGDSIAIG
                     GRLSYPKLWKKAKKIDKRNTKLLSPKTFGGGGADAQPFYEAGIPTLYFNTSGGYKYLH
                     QATDKPETLNPEVFEKVVHLGYLTTMELANKNYKREKDRQKKKAS"
     gene            2327..2914
                     /locus_tag="PLW22_05510"
     CDS             2327..2914
                     /locus_tag="PLW22_05510"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOU74197.1"
                     /translation="MAPDFLQSDLYLYGVLPLLIFFARICDVTIGTLRIIYVSKGQRR
                     IAPILGFFEVFIWIVAISQIMKNLNNFACYFGYAAGFATGNYIGMLVEERLAFGKVLV
                     RVFSTDNGADLVQILNASGFGATSFQAQGAQGEVSIIYSVINRKNLPEVQSLLLNFNP
                     NLFYVVEDIRKVNKGIFQATAQPKISIFERWRKGK"
     gene            complement(2916..3260)
                     /locus_tag="PLW22_05515"
     CDS             complement(2916..3260)
                     /locus_tag="PLW22_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU74198.1"
                     /translation="MNTDLLSWPNGVDFAITICDTAGTILYMNEKSQKVFERWGGKEL
                     IGKNLFDCHSDNSVRIIKELMATGKSNTYTIEKNGVKKLIHQTPWYSNGRVAGMVEIS
                     IETPEGMPHRVR"
     gene            3793..4962
                     /locus_tag="PLW22_05520"
     CDS             3793..4962
                     /locus_tag="PLW22_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU74199.1"
                     /translation="MKKVNSLRTPIYRDSGFWLKDIPTMKKAFADEVNHPADPDLYIY
                     SRYRNPTVVQTEQELAKIEGSAWALLAQSGLAAIDIALSIFQVNAQPVKWLFFSEIYG
                     GTNTFIDKVLVARRGFNIVRFTPTNDSYSLSQFERLMEEQKPDVVFLEAISNPMLIVA
                     DVPEIVKISKRFGAKVIVDNTFATPYILKPLDFGADLVIHSATKYLAGHGNLSAGVVC
                     GNDIDLLKQAIEYRKLVGHMISPDDAYRLSDYLKSFHLRISQHFSNAQRIAEFLSKHP
                     AVEKVLYPSMPYHPTSNIASKIFGNGYGGIVTFDIKGNSFTHKQENCNTFIQLVSHNI
                     HLIPTLGDADTILMPVEPVWGDKYPFPGMVRLSIGIELPESLINVLKNALDKISC"
     gene            5222..5839
                     /locus_tag="PLW22_05525"
     CDS             5222..5839
                     /locus_tag="PLW22_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74200.1"
                     /translation="MQNKLQELTEKIYREGLEKGQAEANQLVTKAKDEAARIVANAKA
                     EAERIIAAARKEADDYRKNIDTEVALSAKQVISGVKQQIVSLIETKLYEAPLVDALKD
                     ADFVKKVIETAINRWEPNSAESVSLRVLVPEEMAKTITDHFTTKTHALLNKEVEIQVD
                     RAIKYGFRIGPKDGSYVVSFTETDFENLFKDFMRPKIVAMLFGGK"
     gene            5826..6704
                     /locus_tag="PLW22_05530"
     CDS             5826..6704
                     /locus_tag="PLW22_05530"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOU74201.1"
                     /translation="MEVSRMMRNYYYLVAGLPELIMDQDRKDFSVNALKQEVKEQVSS
                     SDYRLVEFLHLPYDNDNFLNKLLNRGLEFNDLGSYEKFVFDELDENINQLPQYMQDFY
                     YRFTGKQRSADDAVDQDEQPKDIFEVEKLPEIRFLESFYDLALSQNNDFIRYWFSFTR
                     DFNNVLTALNCRRLGVDPATQLVGNNWLTEVLVKSQAADFGLKREVDYIDRLIQATDI
                     SDVLERERKLDLIKWDMSVEFTTWDYFNINFILGFFVRAGIVHRWLKLDAKTGEELFR
                     KLFDDLKSSYNLSEKF"
     gene            6732..>7129
                     /locus_tag="PLW22_05535"
     CDS             6732..>7129
                     /locus_tag="PLW22_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU74202.1"
                     /translation="MKTQGKVTGIISNLVVVEVDGPVAQNEICYINLAGTKLMAEVIK
                     VIGKLAYVQVFESTRGLRVGTPVEFQGHMLEVTLGPGLLSKNYDGLQHDLDKMKGIFL
                     QRGDYTDALDFDSEWDFTPLVEPGAQVSAA"
     assembly_gap    7130..7229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7230..8257
                     /locus_tag="PLW22_05540"
     CDS             <7230..8257
                     /locus_tag="PLW22_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU74203.1"
                     /translation="QHALAKQANADMIIMAACGERANEVVEIFTEFPHLDDPRTGRKL
                     MERTTIICNTSNMPVAAREASVYTAMTIAEYYRSMGLKVLLLADSTSRWAQALREMSN
                     RLEELPGPDAFPMDLPAVIANFYARAGFVYLNNGSTGSVTFIGTVSPAGGNLKEPVTE
                     STKKAARCFYALSQQRADSKRYPAIDPMDSYSKYLEYPEIQEFLNKNVNETWVDMVMK
                     TKNIIQRGREAFEQINILGDDGVPLDYHDRYWKAEIIDFVVLQQDAFDKIDCNTPLKR
                     QRYMLEKVMQICDTNFHFDGFEQCSSYYKRIINALKQMNYSEFQSEEFYNYEKELETI
                     LNERRVQ"
     gene            8257..9579
                     /locus_tag="PLW22_05545"
     CDS             8257..9579
                     /locus_tag="PLW22_05545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU74204.1"
                     /translation="METKAFQRIYTQLTGITKATVSLRAQGVANEELAMVHGRLAQVV
                     KIMGDMVTLQVFSGTEGIPTNAEVVFFGEPPKLKVGDDLAGRFFDAYGRPMFGDSEIE
                     GEPREIGGPSVNPLRRKQPSELIATGIAGIDLNNTLVTGQKIPFFADPDQPFNQVMAM
                     VALRAQADKIILGGMGLSNDDFLFFKNVFENAGALDRIISFVNTTEQPPVERLLVPDM
                     ALTAAEYFAVEKKEKVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPDGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRTDSDTGKVIVDPFR
                     SLSRLKQLVIGKKTREDHPQVMNAAVRLYADAANAKTKLENGFDLTEYDERALKFAHD
                     YSEKLLAIDVNVEVDTMLNTAWQLFAKYFSKTEVAIKNELVEKYWPAN"
     gene            9595..10197
                     /locus_tag="PLW22_05550"
     CDS             9595..10197
                     /locus_tag="PLW22_05550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU74205.1"
                     /translation="MAIKFQFNKTSLNELNKQLKIRQRALPTLKNKESALRMEVKRAK
                     NRMQELEEHLAKRIKDYEYMVSLWGEFERNLIQIDDVRLGTIKIAGVKIPVLQEVIFS
                     VKEYSVFARPYWYTDGVKILKELAEIGIERDFYHEKMRLLDHARKKTTQKVNLYEKVQ
                     IPGYENAIRKIKRFLEDEENLSKSAMKIVKKRYENVEVES"
     gene            10194..12005
                     /locus_tag="PLW22_05555"
     CDS             10194..12005
                     /locus_tag="PLW22_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOU74206.1"
                     /translation="MITPMYKYNFLVYHRDFDDFLLKLQDLGVVDISMENRNVDEDEK
                     EILARINRIATAIKFLEGRKAPDEKITLSLSADEIVSRVELLQREREQFENAIKKAQK
                     ELAEVEPWGNFDLKLLNSLAEKGISIRFFIAPEKVFNEDWVNEYPIEIVNHTNGLYYF
                     VMVNYTGEAISPDVQEVRTPQFSVKQKDEEIHRYHKRIDEINTELDFLALYIDLLKKE
                     QIELTNQLDFKMVKSSATAEAGNTIMVLEGWVPEDLNEKVKEVLDAESIIYISSKADK
                     NDNAPILLRNSKFAKLFEPISKLFAFPTYAELDLTPFFAPFFMLFFGFCFGDAGYGLI
                     FVLGATIYKFKAKPELKPLLSLIQILGISTVLFGALTGTVFGASLVELDIPALESFKS
                     YFFDQDKMFKLSLILGLVQIVFGMCVKAANLWVQFSWKHAISTISWVVLILSMAVFYL
                     LGQNPESSLKLFSPIHMVIVGVAGVGIMFFNSPGKNPLFNFGLGLWDTYNMATGLLGD
                     TLSYIRLFALGLSSGILGSVFNSLAFGMAPDTPVLKHIVIILILLIGHSINIFMSALG
                     SLVHPMRLTFVEFYKNAGFTGGGRGYNPFRKNTAEAQ"
     gene            12052..12498
                     /locus_tag="PLW22_05560"
     CDS             12052..12498
                     /locus_tag="PLW22_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOU74207.1"
                     /translation="MEPIIFAYLGIGIMIILSGVGSAYGVTIAGNASVGALKKEPGAF
                     GNFLVLSALAGTQGLYGFTGYFMLKKFLIPEITWAQAAAIFGAGLAMGFVGLFSAIRQ
                     GQVCANGITAIGNGHKVFVNTLILAVFPELYAIISLAAVFLISTSL"
     gene            complement(12513..14294)
                     /locus_tag="PLW22_05565"
     CDS             complement(12513..14294)
                     /locus_tag="PLW22_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOU74208.1"
                     /translation="MGFLPTSVKEIKALGWEQPDIIIFTGDAYVDHPAFGAAVIGRVL
                     ESEGFKVAIVPQPNWRDDLRDFKKLGEPRLFFAVTAGNMDSMVNHYTAAKRLRSDDAY
                     TPDGKAGFRPDYATVVYSRIIKKLYASTPLIIGGVEASLRRLTHYDYWSDTLKPSILV
                     DSQADILVYGMGEKAIIEIAKKLDQGMAIESLRGIPQVAYIDHNIDKYKRGEDILILS
                     SYEECLADKLKFANNFKIIEQESNRINSKILIEPIEDRCVVVNPPFNPATTHEIDRWY
                     DLPYTRLPHPRYKGKTIPAWEMIKFSVNIHRGCFGGCSFCTISAHQGRFISSRSEQSI
                     LKEIEEIKKIPGFAGYLSDLGGPSANMYGMGPKDLSICAKCKRPSCIYPNICKNLNSS
                     HEPINNLYNQVRKVEGIKKAFVGSGVRYDLFITRKDSKAEREYFENLFLHHVSGRLKV
                     APEHTSEKVLKIMRKPSFELFKQLKQWFDALNEKFGQKQQLIPYFISSHPGSTEQDMK
                     QLAEETRKLNFKLEQVQDFTPTPMTLASTIYYTGINPYTGEKVYVARSVEEKRKQQSY
                     FFWYKNESKSLFTQSKKRPPKRKPPIR"
     gene            complement(14296..15510)
                     /locus_tag="PLW22_05570"
     CDS             complement(14296..15510)
                     /locus_tag="PLW22_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOU74209.1"
                     /translation="MKVKKISITDYDYELPDSRIAKHPLAERDCSKFLIYQNGEITND
                     TFRNINQYITPKSLLILNNTKVVRARLRFKKESGATIEVFLIEPIEPSDYALSFSSKK
                     KVVWKCIVGNLKRWKQGELQMEIEGTGGRLVAKQLKRLSEGVEVEFLWNNSSMSFADI
                     VEKCGTMPIPPYLNRESEDIDGYRYQTIYAEPEGSVAAPTAGLHFTPKVFESLSKIHV
                     KPHYITLHVGAGTFKPVTSDTIEGHEMHTEHFFVSLELVKRLYQNSEPVVCVGTTTLR
                     TLESLYWLGVKLKLGKLNGNFNISQWEPYEFDFQIDVGEAYKALYEHMKNNGIETLSA
                     STQIIIAPGYEIKTCDALITNFHQPRSTLLLLVAAAVGNDWKKIYQFALDNNFRFLSY
                     GDSSLLHINKST"
     gene            15594..16655
                     /locus_tag="PLW22_05575"
     CDS             15594..16655
                     /locus_tag="PLW22_05575"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOU74210.1"
                     /translation="MNVAVGCKVRFLNDVGGGEVIRIIDKRTALVKTFDGFEMPVLLS
                     ELVVVEPAADELLQKKTVQEKETADANRNKKPKSEVINLTDDTPEVADSSGDELSLDI
                     AFVPSNISNLQQSTFDMYLVNDSTYRVFYVLSNVIDDNHTPISSGILQPDTKVLVKRL
                     TPEDVSKLILMNLQAIYYKNIPYKPFQPEFYDIELDPIKLYKRGNFKRNDFFDIDAYI
                     ISIASTKREAFIRHLTDKAIVEAIKQKEKPVQVKEVKKPNPDTEEVDLHIHELIDNWK
                     DLTPGEILKIQLARFETALEGGIKSTTTKRMVFIHGVGNGKLKYEITKLLSSKYPKLR
                     YQDASFKEYGFGATMVFLK"
     gene            16697..17076
                     /gene="rnpB"
                     /locus_tag="PLW22_05580"
     ncRNA           16697..17076
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW22_05580"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            18236..18661
                     /locus_tag="PLW22_05585"
     CDS             18236..18661
                     /locus_tag="PLW22_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU74211.1"
                     /translation="MVNERLLKLMEQEKVTAAKLADILEVQPSAISHILSGRNKPSFD
                     FIQKLATNFPKLNIDWLITGKGNMYKVAVQQSLFDPPSPLIPKTDNPSSKTNDKSEDL
                     QKSSKVTDVNISVSHKAVKQIVFVYSDNTFEIYSPSKSD"
     assembly_gap    18703..18802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18803..19892
                     /locus_tag="PLW22_05590"
     CDS             <18803..19892
                     /locus_tag="PLW22_05590"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOU74212.1"
                     /translation="KVRLTIFVSFAKKMLYKKLIRPIFFLLNPEFAHKLVVATLKVAF
                     TIPGVRFLCLKMFTVSHPSLQREVFGYRFSNPVGLAAGFDKNADYFPELACFGFSFIE
                     VGTVTPKPQPGNPKPRLFRLIADNALVNRMGFNNKGIDYVKLNLSRKRRPKNLIIGGN
                     IGKNTLTPNQNAIDDYLTVFHGLYPHVDYIVVNVSCPNVANLRDLQDDDSLKSLLKAI
                     NAERMNYDFYKPILLKVSPDLTNEHLTATIKIAEEEGIDGYIATNTTTSRDNLQTPEA
                     KVKTIGNGGLSGKPLAKRSTEVIKLIHTLTQGAKPIIGVGGIHSPNDAIEKLKAGATL
                     IQIYTGFIYEGPAIVKRINKALINIDIA"
     gene            19908..20525
                     /gene="yedF"
                     /locus_tag="PLW22_05595"
     CDS             19908..20525
                     /gene="yedF"
                     /locus_tag="PLW22_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOU74213.1"
                     /translation="MTKIIDCRGQLCPQPLINTKKALKESVKDETIQVLIDNATSCQN
                     VSRFLADNAIPFTVDEQNGLFTLTINPTGSEFNPKKEAEEYCEISFPPKGKKYIVVLT
                     SHLMGNGDNDLGKILMKGFLNTLPSLEGLPEEIICYNSAVTLAVKGSDTAQSLVKLME
                     MGVKITLCGTCVDFFSIKDKIEVGTISNMLYIAERLSSGIHTVKP"
     gene            20528..>21088
                     /locus_tag="PLW22_05600"
     CDS             20528..>21088
                     /locus_tag="PLW22_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU74214.1"
                     /translation="MANYYFDNSATSHPKPPEVAQYINEYLKLGGTYGRSAYDRVINV
                     SRIVEHTRILLARLLGTSLTEHIVFTYNATHALNIAIKGFSYRKKRVLISPLEHNAVT
                     RPVFHLQNTIGLEFDIMPYNPDGSINLEGLRQLDLKSYDLCIVNHVSNVNGVVQPIAE
                     LKALLGDVPLLVDASQSAGHIPIEVDT"
BASE COUNT         5995 a         4221 c         4278 g         6394 t
ORIGIN      
        1 taattattag cgcgctcttt ggggtgctct gaaaaaagtt ttgcaaaacc ttaaaattta
       61 ttatcaacct tattataagg gttgatggtt ctaaaatcgc ttggaatttt acatttcttt
      121 tcctttttcg gttttcatta aaacattttg tcccattttg tgtttacctt ttacaactaa
      181 aaagtaaaca tcatgaaaat tttaggtatg ataattctgt tggctacaac acttagcacc
      241 acaaatgcac aaaatgtaag tttccatagt tttgtggtaa ccgatatcga tggcaacgag
      301 tttcctctct caaagctgaa gggaaaaaag gtaatggttg ttaatactgc atcaaaatgt
      361 gggtttacac cacagtacga ggatttacaa gcgctttatg ataagtacaa ggataaaaac
      421 tttgttatta ttggatttcc ggccaataat tttatggggc aggaacctgg aaccaatcag
      481 gaaattaagg agttttgcac tactcgtttt agtgtatcct tccctatgat ggcaaaaata
      541 tcggttaagg ggaatgacac ccatcccctg taccagtggc ttacccaaaa aagtatgaat
      601 ggtgtaatgg atagcgaggt taaatggaat ttccagaagt acctgataga cgaaaatgga
      661 aaacttgtcg atatgatacc accaaaggaa aaaccaaaca gcgataaggt gattaaatgg
      721 attgaaggga attaagtaaa aaggcctcac ttacgaggcc tttttctttt gtctgtcctt
      781 ctctcgttta taatttttat tggcaagctc catagtggtt aggtacccta aatgtacaac
      841 cttttcaaaa acttcagggt taagggtttc tggcttatca gtagcttgat gcagatactt
      901 gtatccacca ctggtgttga agtaaagcgt aggaatacct gcttcgtaaa aaggttgggc
      961 atcggcacca ccgccaccaa atgtttttgg gcttagtagt ttggtgtttc ttttatcaat
     1021 ctttttggct tttttccaaa gcttcggata gctcaacctg ccaccaatgg caatgctatc
     1081 gccttgccca atacagtcaa agttgagcat ggctaccacc ttatcggcat taatgggcat
     1141 atttgcaaca aaatatttcg atccttttaa gccggattct tctgacgaaa aggccacaaa
     1201 aacaactgaa cggaggggct tcgtagttga tttgtttatt gcttcggcaa ttgcaattag
     1261 cccggcaacg cccgatgcat tatcatttgc cccaggaaag taaagcttat cgccctggta
     1321 gccaacatgg tcaaggtgcg ctcccaggat aatatattca tgcttaagct tagggtcaga
     1381 gccttcaata tagcctacaa cattaaaagt cttttttgct tcctggtaaa cggctcgtac
     1441 gttgatatag atcgatttat cggtttcaac ggagtggggg gctttgtcgc gttttatcat
     1501 ttcatagaaa tcggcgggtt tgtagggtaa cttgctaaaa aggctatcgg taaacctact
     1561 gccggcatgt atcattggga aatcagctag atgtggctta gggccacagg ctatgctccc
     1621 atataccatg gtgtttggtg tcatgtgggg ttcaccgata aagattatgg ctttagcacc
     1681 taattctttt gctattctag cctttccccg tggcgataca tcgccccaac taccggaact
     1741 ggcttgccag ggtggggtcg atttgaatac tataacaatt ttattgcgga catcaatact
     1801 tcggtaatcg ttgtattcat cattttttat accaaatccg gcaaaaacag tttcacctcg
     1861 tacttcccct gaaccggtaa atcctctaca tacaaaatcc tctcctaaat acatgggttg
     1921 tgtagggcga ttgttctgat ctaatagaaa tacccttgca tccagtatgt agttaacttc
     1981 ttcatcaaac tcctgcaaca tggtgttccc caacaatgga tttaaaccgg catttttaaa
     2041 ccttgtagca acgtaacgtg aggcctgctc atattctacc gaaccaggca gccgcccttt
     2101 aaatcgctct gacgataaga atttaacggt gttcatcaaa tccttgctgt cgataggact
     2161 ttgccgaatt tttgtttggc ttatagccaa atcatgggtt actagtattc ctaccagaaa
     2221 aacaagatat ttccacatcg cattcatggt tttagagcct aaatttagaa attttatggt
     2281 aatattttat ttgcgtaaat ttgcagagca atagtaatat tttcaaatgg ctcccgactt
     2341 tcttcaatcc gatttatatc tgtatggtgt tttaccattg ctcatctttt ttgctcgaat
     2401 atgcgatgta accataggga cattgcgtat tatttacgtt tcaaagggtc aacgccgcat
     2461 tgctcccatt ttaggatttt ttgaggtgtt tatctggatt gttgccataa gccaaatcat
     2521 gaagaacctc aacaattttg cctgttactt tggctatgcc gctggttttg ctacaggcaa
     2581 ctatattggc atgctggtgg aggagcgatt ggcttttgga aaggtattgg tcagggtgtt
     2641 ttcaaccgat aatggagccg atcttgttca gatcctcaat gccagcggat ttggtgcaac
     2701 atcctttcag gctcagggcg cacagggtga ggtaagtatt atttactccg ttattaaccg
     2761 gaagaatctc cctgaggttc agagtcttct tttaaacttt aacccaaacc ttttctatgt
     2821 ggtagaggat ataagaaagg taaataaggg aatttttcag gcaacagctc agccaaaaat
     2881 atccattttt gagcgttggc gcaagggaaa gtagattacc ttaccctatg aggcatccct
     2941 tccggtgttt caattgaaat ttctaccatt cctgccactc taccgttgct gtaccaagga
     3001 gtttggtgta ttagtttttt aactccattc ttttcaatgg tatatgtgtt tgattttcct
     3061 gtggccataa gctctttgat tattcttact gaattgtcgg aatggcagtc aaaaagattt
     3121 tttccaataa gttcctttcc tccccagcgt tcaaaaacct tttgcgattt ttcgttcatg
     3181 taaagaatag ttcctgctgt atcgcaaatt gttatggcaa agtctacacc atttggccag
     3241 cttaataaat ctgtattcat tgaagtgctt tttttacaag gcaaaggtaa tcttaaaccg
     3301 agattagtca atagtaacac aataaaacat ttaaacagct tgtttctact ttacttatag
     3361 gggtaatccc gacttatcaa aacctttcat tccgagatct cggaatgatt agattttgtt
     3421 agtagattcc tcaattgaaa attaaattgt gaatattctc taaaaatgtt tacttatccc
     3481 catttttttt caatccattt taatttttta tttcatggta cttaggtttt gctgttcact
     3541 tctcactttt catttttcac atttcgcttt ttactattcc tctaacttcc tctaacttcc
     3601 tctaacttcc tctaacttcc tctaactcca tcatattctg tctattccga ctcattcccc
     3661 taaaattagc ctggttgaat ttgtttattc atggtacttg ggttaaattc taatggtgat
     3721 tgagtttaat gatagaaaca aacgagcggc tcaaattttt tactatcttg catggagtaa
     3781 aaaaaatgat agttgaaaaa ggtaaattct ttacgtacac ccatttacag ggattcagga
     3841 ttctggctta aggacatccc aactatgaaa aaggcgtttg ccgatgaggt aaaccaccct
     3901 gctgacccag acctatatat ctactcccgc taccgtaatc ccaccgttgt tcaaactgaa
     3961 caggaacttg ccaaaatcga aggttctgct tgggctctcc tagctcaatc aggattagca
     4021 gcaattgata ttgccctttc catttttcag gtaaatgctc aaccggtgaa gtggctgttc
     4081 ttctctgaaa tttacggggg tactaatacc tttatcgata aggttttagt tgcgcgtcgc
     4141 ggattcaata ttgttaggtt tacaccaacc aacgattcat attcgctcag ccagtttgaa
     4201 aggttgatgg aagagcaaaa gcctgatgtg gtgttccttg aagctatttc aaaccccatg
     4261 ctcattgttg ccgatgtgcc tgaaatagtt aagatttcaa agcgatttgg ggcaaaggtt
     4321 attgtcgata acacatttgc aacaccatac atcctaaaac cgttagattt tggtgccgat
     4381 ttggttatcc atagtgcaac caaatacctg gcagggcacg gcaacctctc agccggagtg
     4441 gtttgtggta acgatatcga tttgcttaaa caagcaattg agtaccgcaa gctggttggc
     4501 catatgattt cccctgacga tgcctatcgg cttagcgatt acctgaaatc gtttcacttg
     4561 cgtatttcac agcatttttc gaatgcacag cgtatcgcag agtttctttc aaagcaccct
     4621 gcggtggaaa aagttctcta tccctcaatg ccctatcatc caacaagcaa cattgctagc
     4681 aaaatatttg gcaatgggta tggtggaatt gttacctttg acattaaagg caattccttt
     4741 acccataaac aagagaattg caacactttt atccagttgg tttcgcacaa tattcatctc
     4801 attccaaccc ttggagatgc tgacacaata ctgatgcccg tggaaccagt gtggggcgac
     4861 aagtatcctt tccctggaat ggttaggttg tcaattggca ttgaactccc cgagagctta
     4921 atcaatgtgt taaaaaatgc actggacaaa ataagttgtt aacatgctaa cgattgcaaa
     4981 aagttaaaat attttaatgt tatattgata acaagtattt tgcttatgat tattttattg
     5041 attattaggt caatgcgcta taaaaacatt gatttgattg ctaattttta ggtaaaatca
     5101 taacaattgt catatttttg cctataacct aaacattatt tttgccgtaa caggttgcag
     5161 taatttgaac caacataatc accgaaaacc ctaaaaacac ataatacagg aggaataagc
     5221 tatgcagaat aagcttcaag agttaaccga gaagatatat cgcgaaggtc tggaaaaggg
     5281 acaggctgaa gctaatcagc ttgtgaccaa ggctaaggat gaggcagcac gtatagtggc
     5341 caatgcaaaa gctgaagccg aaagaattat agccgctgcc cgcaaggaag ccgacgatta
     5401 ccgcaaaaat atcgataccg aggttgctct ttccgccaag caggttatca gcggggtcaa
     5461 acagcaaata gtttccttaa ttgaaaccaa actttacgaa gccccactgg ttgacgccct
     5521 aaaggatgcc gattttgtga aaaaggtaat tgaaactgct attaaccgtt gggaacctaa
     5581 ctcagctgag agcgttagcc tgcgtgtact tgttcccgaa gaaatggcta aaaccattac
     5641 cgaccatttc accaccaaaa cccatgcatt gctcaacaaa gaggtcgaaa ttcaggttga
     5701 tagggctata aagtatggtt tccgcattgg tccaaaggat ggtagctacg tggttagctt
     5761 taccgaaacc gattttgaga acctctttaa ggacttcatg cgtccaaaaa tagttgcaat
     5821 gctttttgga ggtaagtaga atgatgcgaa actactacta cctggtggct ggcctcccgg
     5881 aactgattat ggatcaggac cgaaaggact tcagcgtaaa cgctttgaaa caggaagtta
     5941 aggaacaggt ttcctcttcc gattaccgcc tggtagagtt cctgcacttg ccctacgata
     6001 acgacaactt tctcaataag ctgctcaacc gtggacttga gttcaacgac ttgggctcct
     6061 acgagaaatt tgtttttgat gagctcgatg agaatattaa tcagctccca caatacatgc
     6121 aggattttta ctaccgcttt acaggcaaac agcgctctgc cgatgatgcg gttgaccagg
     6181 atgagcagcc aaaggatatt tttgaggttg aaaaacttcc tgagattagg ttcctggaat
     6241 ctttttacga ccttgccctt agccaaaaca acgattttat aaggtactgg ttcagcttca
     6301 ctcgcgattt taacaatgtg cttaccgcgc taaactgtcg caggcttggg gtcgatcctg
     6361 ccacccaact ggttggtaat aactggctta ctgaggttct ggttaaaagc caggccgctg
     6421 attttggcct aaagcgcgaa gtcgactata tcgataggct gatccaggct accgatatct
     6481 ctgatgtttt ggagcgtgaa cgtaagcttg acctaattaa gtgggacatg tctgttgagt
     6541 ttactacttg ggactacttt aatatcaact ttatacttgg tttctttgtt cgtgccggta
     6601 ttgtccatcg ttggctaaag ctcgatgcca agaccggtga ggaacttttc aggaaacttt
     6661 tcgatgatct taagtcatcc tacaatctct ccgagaaatt ttaaagcgat tattaattaa
     6721 caattaacat aatgaaaact caaggaaaag taaccggaat aatttcgaac cttgtggttg
     6781 ttgaagtcga tggcccggtt gcccagaacg agatttgcta tatcaatctc gctggcacaa
     6841 agcttatggc cgaggttatc aaggttattg ggaaactggc ttacgtgcag gtgttcgaga
     6901 gcactcgcgg ccttagagta ggaactcctg ttgagttcca ggggcacatg ctcgaagtta
     6961 ctcttggccc tggcctcctc tccaaaaact acgatgggct tcagcacgac ttagataaaa
     7021 tgaagggcat tttccttcaa cgaggcgatt atactgacgc cctcgatttt gactcggaat
     7081 gggattttac tccacttgtt gagcccggtg ctcaggtaag cgccgccgan nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt acagcacgcc ttggctaaac aggctaatgc
     7261 cgatatgatt attatggcag cctgcggtga gcgtgccaat gaggtggttg aaatttttac
     7321 tgagtttcct cacctcgatg acccacgtac aggtcgtaag ctaatggaac gtactaccat
     7381 aatttgtaat acatctaaca tgccggtagc tgcccgtgag gcatcggttt acaccgcaat
     7441 gactattgct gagtactacc gctccatggg cttaaaggtt ctgcttttgg ccgactctac
     7501 atcccgctgg gctcaggctc tgcgtgaaat gagtaaccgt cttgaggaac ttcctggtcc
     7561 cgatgctttc ccaatggacc ttccagctgt aattgccaac ttctacgccc gtgctggttt
     7621 cgtttacctc aataatggca gcactggttc agttactttc atcggtaccg tttcgcccgc
     7681 tggtggtaac cttaaggagc ctgttaccga atcaaccaaa aaggctgccc gctgtttcta
     7741 tgcgctctcg cagcaacgcg ccgatagtaa acgttatcca gccatcgacc caatggatag
     7801 ttactctaag taccttgagt accctgaaat tcaggaattc ctgaataaga atgtcaatga
     7861 aacctgggtt gatatggtaa tgaagacaaa gaatatcata cagcgaggta gggaagcctt
     7921 tgaacaaatc aacatacttg gtgacgatgg cgttcctctc gactatcacg atcgttactg
     7981 gaaggccgag atcattgact ttgttgtatt gcaacaggat gctttcgata aaattgattg
     8041 taatacgcca ttgaagcgcc agcgatatat gcttgaaaag gttatgcaga tatgtgatac
     8101 taacttccat ttcgatggtt ttgagcaatg ctcatcgtat tacaaacgca ttatcaacgc
     8161 gcttaagcaa atgaactact ctgagttcca gtccgaagag ttctataact acgagaagga
     8221 acttgaaaca attcttaacg aaagaagggt acagtaatgg aaactaaagc atttcagaga
     8281 atatacaccc agctaacggg tatcacaaag gcaaccgtat cgcttcgggc ccaaggtgtg
     8341 gctaacgagg aattagctat ggttcacggt cggttagccc aggttgtaaa aattatgggt
     8401 gatatggtta ccctacaggt gtttagcggc actgagggta tccctaccaa tgccgaggtt
     8461 gttttctttg gcgaaccccc aaaacttaaa gtaggcgacg atttggcagg gcgctttttt
     8521 gacgcctatg gccgtcccat gtttggcgat tcggagatcg agggtgaacc ccgtgagatt
     8581 ggtggccctt cggttaaccc ccttcgccgt aaacaaccct cggagctcat tgctaccggt
     8641 attgcaggta tcgacctaaa caatactctt gttactggtc aaaagatacc tttctttgct
     8701 gaccccgacc agccttttaa ccaggttatg gctatggtag cactccgggc tcaggccgat
     8761 aaaatcattc ttggcggcat gggactctca aacgacgact tccttttctt caagaatgtg
     8821 ttcgaaaatg ctggtgcgct cgaccgtatc attagctttg ttaatactac tgagcagccc
     8881 cctgttgaac gcttgcttgt gcccgacatg gcattaaccg cagccgagta ctttgcggtt
     8941 gagaaaaagg aaaaggtgct tgttcttcta accgatatga ccctctacgc cgatgcgttg
     9001 agtattgtat cgaaccgtat ggatcaaatc ccttcaaagg actccatgcc aggatcgctt
     9061 tattccgacc tagcaaagat atacgagaaa gccgttcaat tccccgatgg tggttcaatc
     9121 accattattg cagtaactac actatcgggt ggtgatatta cacatgctat ccccgataac
     9181 actggttaca ttaccgaggg acagctcttc ctccgtaccg atagcgatac aggtaaggtt
     9241 atcgttgacc cgttccgaag cctctcgcgt ttgaaacagc ttgtaattgg taagaaaact
     9301 cgcgaggatc atcctcaggt tatgaacgct gcagttcgtc tgtatgccga tgctgcaaat
     9361 gcaaaaacca agctggaaaa cggcttcgac cttactgagt acgatgagcg tgcccttaag
     9421 tttgcccacg actattccga aaagttgctg gcaattgatg ttaacgtaga ggttgatacc
     9481 atgctcaata ctgcatggca gctctttgct aagtatttct ctaaaaccga agtggccatt
     9541 aagaacgagc tggttgagaa atactggcct gcaaactaat aatgtgctaa aaacatggca
     9601 attaagtttc agttcaataa aacatcgctt aacgagctca ataagcagct taaaatccgc
     9661 caaagagctt tgcctaccct aaagaataag gagtcggctt tgcgtatgga ggttaagcgc
     9721 gctaagaata ggatgcagga actcgaggaa catctggcta aacgcatcaa ggattatgag
     9781 tatatggtta gcctttgggg agagtttgag cgtaacctga ttcagattga cgatgttagg
     9841 cttggaacta tcaaaattgc cggggtaaaa atacctgttc tacaagaggt aatttttagt
     9901 gttaaagagt atagcgtttt tgcgcgcccc tactggtaca ccgatggagt aaaaattcta
     9961 aaggagctag ccgaaattgg tattgaacgc gatttctacc acgaaaaaat gcggctcctt
    10021 gaccatgcgc gtaagaaaac tacacaaaag gtaaaccttt acgaaaaggt tcaaattccc
    10081 ggctatgaga acgccattcg gaagattaag cgattcctcg aagatgagga aaacctttcg
    10141 aagtcggcta tgaagatagt gaagaaacga tacgaaaacg tggaggttga gtcatgataa
    10201 caccaatgta taaatacaac ttcctggttt atcaccgcga tttcgacgat ttcctgctta
    10261 aacttcagga tttgggcgtg gttgatattt ccatggaaaa ccgaaacgtt gacgaggatg
    10321 aaaaggagat tctggcccga atcaatagga tagctactgc tatcaagttt cttgaaggcc
    10381 gaaaagcccc tgacgaaaaa atcacactca gcctttcagc cgatgaaatt gtttcgcgtg
    10441 ttgagttgct tcaacgtgaa cgggagcaat ttgaaaatgc tattaaaaaa gcccaaaagg
    10501 aattggcaga ggttgaaccg tggggtaatt ttgacttgaa attgcttaac agccttgccg
    10561 aaaaaggtat ttcaattcga tttttcattg cacccgagaa agtttttaat gaggactggg
    10621 taaatgagta tcctattgaa atagttaacc acaccaatgg actatactac tttgtaatgg
    10681 ttaattacac aggcgaggcc atttcgcccg atgttcagga ggttcgaaca cctcagttct
    10741 cagttaagca aaaagatgaa gaaatacatc gctaccataa acgtatcgat gaaattaaca
    10801 ccgaactaga ttttctggct ctttacatcg atttgcttaa aaaagagcag attgaactta
    10861 ccaatcagct cgattttaaa atggttaagt caagcgcaac ggccgaagca ggaaatacca
    10921 taatggtact tgagggatgg gtgcctgagg atttgaatga aaaggtaaaa gaagttcttg
    10981 atgccgaaag tattatctac atttcgtcta aggccgataa gaacgataac gcgcctattc
    11041 tacttcgaaa cagtaaattc gcaaagctgt ttgagcccat tagtaaactt tttgccttcc
    11101 caacctatgc agaattggac ttaaccccat tctttgcacc attcttcatg ctcttctttg
    11161 ggttctgctt tggcgatgcg ggttacggtt taatctttgt cttaggtgcc acaatctata
    11221 agtttaaggc aaaaccagaa cttaaacctc ttctttcttt aattcagatt cttggaatat
    11281 ctactgtgtt gtttggagca ctaaccggta ctgtgtttgg cgctagcctg gttgagttag
    11341 atattcctgc tttggagagc tttaaatcct acttctttga ccaggataag atgtttaagt
    11401 tatcccttat acttgggctt gtgcagattg tttttggcat gtgcgttaaa gccgctaacc
    11461 tttgggtaca gttttcatgg aagcatgcca tttctactat tagttgggtt gtattaatcc
    11521 tcagcatggc tgttttctac cttttaggcc aaaaccccga aagttcattg aaacttttca
    11581 gcccaatcca tatggttatt gttggtgttg cgggcgttgg gataatgttt tttaactcac
    11641 ccggtaaaaa tcccctattc aactttggat taggcctttg ggatacttac aacatggcta
    11701 caggcctttt aggcgatacc ttgtcataca ttcgtctctt tgcccttggt ctgagtagcg
    11761 gtatcctagg tagtgtgttt aatagccttg cctttggtat ggcccccgat actccggtgc
    11821 tcaagcatat tgttattatt ctgatattgc ttattggcca ttccattaat attttcatgt
    11881 ctgcattagg ttcacttgta catcctatgc gtttgacatt tgttgagttt tacaagaatg
    11941 ctggctttac aggcggtggt agagggtaca atcctttccg taagaatacg gctgaagccc
    12001 aataaatttg aaatcgattt ataatttaaa taacctaata aattttaaac tatggaacca
    12061 atcatttttg cttacttagg aattgggata atgattatcc tttctggtgt tggaagtgct
    12121 tatggtgtta ccattgctgg taacgcatcg gttggcgctt tgaaaaagga acctggtgca
    12181 tttggtaact tccttgttct tagcgcgctt gcaggaacac agggtcttta tggctttacc
    12241 ggttacttta tgctcaagaa attccttatc cctgaaatca catgggctca agctgctgca
    12301 atttttggtg caggtttggc catgggattt gtagggcttt tctcagcaat tcgccaggga
    12361 caggtttgtg ctaacggtat tactgccatt ggtaatgggc acaaggtgtt cgtaaatacc
    12421 cttatccttg ctgtattccc agaactttat gcaattatct ctttggctgc tgtatttcta
    12481 atcagcacct cattgtagtt gaaattcttc acttatctaa taggcggctt ccttttgggt
    12541 ggccgctttt tactttgggt aaaaagggat ttactttcgt ttttatacca aaagaaatag
    12601 ctttgctgtt tgcgtttttc ttcaacgctt cgggctacgt aaaccttttc gccagtatag
    12661 ggatttatac ctgtatagta aattgttgat gctagggtca tgggagtggg cgtgaagtcc
    12721 tgaacctgtt caagcttgaa gtttagctta cgggtttctt cggctaactg cttcatgtct
    12781 tgttctgttg accctggatg gctcgatata aagtatggaa ttaattgctg tttttggcca
    12841 aatttttcgt tcaaagcatc aaaccactgc ttaagctgtt taaacagttc aaacgatggt
    12901 tttcgcatta tttttagtac tttttccgat gtatgttcgg gtgccacctt taaccgcccc
    12961 gatacatgat gtagaaatag gttttcaaaa tattcccgct cggctttact atccttccga
    13021 gttataaata aatcgtagcg taccccgctg cccacaaacg ctttttttat tccttctact
    13081 ttacggactt gattgtatag gttgtttata ggctcatggc tcgaatttag atttttacag
    13141 atattcgggt agatacacga aggccgtttg catttggcac aaatgcttaa atcctttgga
    13201 cccatcccat acatattagc cgatggccca ccaaggtcac tgaggtagcc tgcaaaacca
    13261 gggatttttt ttatttcctc aatctctttc aggatagatt gctctgaacg cgacgaaata
    13321 aaacgtccct gatgggccga tattgtgcag aaactacacc ctccaaaaca cccccgatgt
    13381 atgtttactg agaatttaat catctcccac gcaggaatgg ttttcccttt gtatcgcgga
    13441 tgtggtaatc gggtataagg taaatcatac catctatcaa tttcatgggt tgtggcagga
    13501 ttaaagggcg gatttacaac tacacaccta tcctctatgg gttctataag aattttactg
    13561 tttatcctgt tcgattcctg ctcaattatc ttaaagttat tggcaaattt gagtttgtcg
    13621 gctaaacact cctcgtatga acttagtatt agaatatcct ccccacgttt atacttatca
    13681 atattgtggt caatgtaagc aacttggggt atcccgcgta gcgattcaat tgccataccc
    13741 tggtcaagct ttttagcaat ttcaataatg gctttttcac ccattccata aaccagtata
    13801 tcggcttggc tatcaactaa aatacttggt ttgagggtat ctgaccagta atcataatga
    13861 gttaatctac gcagcgaagc ctctacacca ccaataatca aaggggtgct tgcatatagt
    13921 tttttgatta ttcgggagta tacaactgtg gcataatcgg gtctaaaacc tgctttacca
    13981 tctggggtat aggcatcatc gctccttaat cttttagcgg ccgtatagtg gtttaccatg
    14041 gagtccatat taccggcagt aacggcaaag aaaagccttg gctctcctaa ttttttaaag
    14101 tcacgcaggt cgtcgcgcca gttgggttgg ggtacaatag caaccttaaa tccctcgctt
    14161 tccagtactc gtccaataac agcagcgcca aatgcaggat gatcaacata ggcatcacct
    14221 gtaaatatta tgatatcggg ctgttcccag cccaatgctt ttatttcttt taccgatgtg
    14281 ggcaaaaatc ccattttatg ttgatttgtt tatatgaagt aaactactat cgccatagct
    14341 taagaaccta aaattgttgt ctaaggcaaa ttggtatatt tttttccaat cgttgccaac
    14401 tgcagcagca acaagtaata gaagggtaga gcgtggttgg tgaaaattgg ttattagggc
    14461 atcgcaggtt tttatttcat aaccgggagc aataataatt tgtgttgaag ccgataaagt
    14521 ctcaatgcca ttgtttttca tgtgttcgta aagagcctta taggcttctc caacgtctat
    14581 ttgaaaatca aactcatatg gttcccattg cgaaatgtta aagttcccat ttaacttgcc
    14641 aagttttaat ttaaccccaa gccaatacaa cgattccaag gttcttaagg tagttgttcc
    14701 tacgcataca accggctcgc tattttgata tagtcttttt actagttcta gactaacaaa
    14761 aaagtgttcg gtatgcatct cgtgaccctc aatggtatcg gatgttacag gtttaaaagt
    14821 gcctgcacct acgtgtaggg ttatgtagtg gggtttaaca tgaattttac ttaaactttc
    14881 aaaaaccttt ggtgtaaaat gaagaccggc agttggcgct gctaccgatc cttcgggctc
    14941 tgcatatatg gtctgataac ggtagccgtc aatatcctcg ctttcacggt ttaggtaagg
    15001 gggaatgggc atagtaccgc atttttccac aatatcggca aatgacatag aactgttgtt
    15061 ccacaagaac tcaacttcta cgccttcgct aagccttttt agctgttttg ccacaagcct
    15121 tcctccagta ccttctattt ccatttgaag ttcgccttgc ttccaacgct taaggttgcc
    15181 cacaatgcat ttccatacca cctttttctt gctcgagaac gaaagagcgt agtcacttgg
    15241 ctcaatgggt tcaattagaa atacttcaat tgttgcccca ctttcctttt taaaccttaa
    15301 ccttgctcta accaccttag tattgttcag tataagtaag ctttttgggg taatatattg
    15361 gttaatatta cggaaggtat catttgttat ttcaccgttc tggtaaataa gaaatttaga
    15421 gcagtccctc tcagcaagag gatgtttggc tatccttgaa tcgggcaact cataatcata
    15481 gtctgtaata cttattttct taactttcat atcccttttt tgactctaag gccacaaaat
    15541 tagttatttt tgtgggttaa aaagatttgt gagtaattct gaggagtttt attatgaatg
    15601 tagctgtagg atgtaaggtt cgttttttaa acgatgttgg agggggtgag gttattcgga
    15661 tcatcgataa gcgaacggct ttggtcaaaa ctttcgatgg ttttgagatg ccagttcttt
    15721 tatctgagct ggtagtggtt gaaccggctg ccgatgaact cctgcagaaa aaaacagttc
    15781 aggagaagga aacggctgat gcaaatcgca acaaaaagcc aaagtctgaa gttatcaatc
    15841 taaccgatga cacaccagag gttgctgata gttcaggtga tgaactctcg ttggatatag
    15901 cttttgttcc tagtaatata tccaacctac agcaatccac tttcgatatg tatttggtta
    15961 acgatagcac ctatagggta ttctatgtac tttcaaatgt gattgacgat aatcataccc
    16021 caattagctc aggcatactt cagcccgata caaaggtatt ggttaaaagg ctaacccccg
    16081 aagatgtaag taaactgatt ttaatgaacc tacaagcaat atactataag aacatcccat
    16141 ataagccatt tcagccagaa ttctacgata ttgagcttga ccctataaaa ctatacaaaa
    16201 ggggcaattt taaacgcaat gatttttttg atatcgacgc atacataatt tccattgcaa
    16261 gcactaagcg cgaggctttt attcgtcact taaccgataa ggcaatagta gaggcaataa
    16321 agcaaaagga aaaaccggtt caagtaaaag aggttaagaa accaaatcct gatactgaag
    16381 aggtagatct tcacattcat gagcttatag acaattggaa ggatttaaca ccaggtgaaa
    16441 tcttgaaaat tcagcttgct cgtttcgaaa cggctcttga gggaggcatt aaaagcacaa
    16501 ctactaaacg tatggttttt attcacggag taggcaatgg gaagttaaag tacgaaataa
    16561 ccaagctgtt aagcagcaaa tacccaaagt taaggtatca ggatgcctcg tttaaagaat
    16621 acggctttgg tgcaaccatg gtattcctta agtagtataa agggaaaaga gttttttgaa
    16681 accataaaat aggcaagcag atagccgtcc tatcgcgccg tgcaatatcg gtgaggaaag
    16741 tccgggcagc gcagggcacc atacttccta accggaagat gtccgcgagg gcatagcagt
    16801 gcaacagaaa ataaccgcca tgcccttggc ttggtaaggg tgaaaaggtg aggtaagagc
    16861 tcaccggatt ccatagcgat atggaatgcc gtgcacctta tgggctgcaa gaccatgtat
    16921 actccgactt cttacgaaac agggctgccc gttctgttcc cgcttgtcgg gtgtcggagg
    16981 gggtaggtcg cttgaggttg taggtgacta caatcccaga taaatgatag gagcgttttt
    17041 aacgcacaga acccggctta caggttatct gcttgccttt attattttaa tttgctatta
    17101 attatgttaa gttttagcaa gttcttttta gataagtaaa atgaagaagg gaatatcttg
    17161 tatatacttt ttactatcat cttaaaatcc cccaaaaaaa aggataaata ctaaaaatta
    17221 acagccccca cacctagatt tacaaatgta aacatgggtg ctttcagaaa aagatcattt
    17281 ttaaaaacaa tagaaattaa tggttgatac atcaaatata tattaatata ttatttatat
    17341 aactgtatat caataattaa tatgttttat attatttaat tatttaaaat atattggtct
    17401 atacacatat tatagaataa atattagtta aaatattgaa atatactgtt caatatcaat
    17461 tatatacaat atttatttca agtaaatatt tttaaccata atgttagctg gtttagtacc
    17521 agtaattttt tcataaacgt aatagcaaga ggagtgttgc cccatacgtt aatcagcaat
    17581 acctgtaaaa tcaactatta ctaaacaaaa caagccaaag cattaaaccg tagtggggta
    17641 gtagttatga cttttactta tgatggttac ttaattacgt atataagagc cttaggaaat
    17701 ctttttgagt ttgttgatta cagcgatagt aaataatgaa gggtttttgt gccttaacat
    17761 ctatatgaaa taccgataat tatataccgt tcagttctgt tggttaaggt taattatcct
    17821 gttgatagca aaattttagt tcacaggatt aatcttgttt caacttttat acgaatgcct
    17881 aatggtatgg cctgtatgtc gtttaatgct aaatcatttc tttgttgatg tcttaaattt
    17941 ggcgtttaat ttaaaggtat acttaaccta tgggtgaaac acctctaagg ggattacttc
    18001 attgaaaata tagccacgtt aagaagttga taatattcat tcacctcctt ttaaaattaa
    18061 tttgtttcgc aatcggattg ctttaccaaa aagcaaatac acaacgctgc gtaaccttgg
    18121 gttacatgtg tgaatgtgct tatttttaca atatttacat ttgtattttg acaatacttt
    18181 acgaataatt acatttgtga ataattcaag tggtacaatc aaaaacattt caattatggt
    18241 taacgaaaga ttgcttaagc ttatggagca ggaaaaggta actgctgcca agctagccga
    18301 tatattagag gttcaaccca gcgctatttc ccatatactt tcagggcgta ataagccaag
    18361 ttttgacttt attcaaaagc tagccacaaa tttcccaaag ttgaatattg attggcttat
    18421 aaccggcaag ggtaatatgt acaaggttgc agttcaacag tcacttttcg atccgccatc
    18481 acctttaatt cctaaaaccg ataatcccag ctctaaaacc aacgataaaa gtgaagattt
    18541 gcaaaaatca tcgaaagtta cagatgtaaa tatttcggtt tcacacaaag ctgtaaagca
    18601 aattgttttt gtttattcag ataatacttt tgaaatatat tccccctcaa agtccgatta
    18661 actatttttg ttagttttgc aaaaaaaatg ctttacaaga agnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nncaaagtcc gattaactat ttttgttagt tttgcaaaaa
    18841 aaatgcttta caagaagtta atcagaccaa tatttttcct tttaaatcct gaatttgctc
    18901 ataagttagt ggtagcgacg cttaaagttg catttaccat tcccggggtg cgttttcttt
    18961 gcttaaaaat gttcacggta tctcaccctt cgcttcagcg cgaggtcttt ggctatcgtt
    19021 tttccaatcc cgtagggctt gccgctggat tcgataagaa tgctgattac tttcccgagt
    19081 tagcctgttt tggtttttca tttattgagg ttggtactgt tacaccaaaa cctcaacctg
    19141 gaaatcccaa accacgttta ttccgtttaa ttgccgacaa tgctttggta aaccgaatgg
    19201 gattcaataa taaaggtatt gattatgtta agttgaacct gtcaagaaag cgtcgtccaa
    19261 agaatttaat aataggtggt aacattggaa agaatacgtt aacgccaaac caaaatgcaa
    19321 tcgatgacta tcttactgtt ttccatggtt tgtaccccca tgtggactac attgtggtta
    19381 atgttagttg tcctaacgtt gcaaatctgc gcgatttgca ggacgatgat agtctgaaaa
    19441 gcttacttaa ggcaattaat gccgaacgaa tgaactacga tttctataaa ccaatacttc
    19501 ttaaggtttc accggatctc accaatgaac atctgactgc cacaattaaa attgctgaag
    19561 aagaaggaat tgatgggtat attgcaacca atactacaac atcacgcgat aaccttcaaa
    19621 cccctgaagc aaaagttaaa accattggga atggaggttt aagcggtaag ccattggcta
    19681 aacgttctac agaagtaatt aagcttattc atacccttac ccagggggca aaacccatta
    19741 ttggggttgg tggcattcat tctcctaacg atgcaataga gaaactgaaa gccggtgcta
    19801 cactcattca gatttacaca gggttcatct acgagggacc ggctattgtt aagcgcatta
    19861 ataaagcatt gataaatatc gatatagctt aaacttaaga aaccaatatg acaaaaatta
    19921 ttgattgtcg tggacagcta tgccctcagc cacttatcaa tactaaaaag gctctgaaag
    19981 aatcggttaa ggatgaaacg attcaggttt taatagacaa cgcaacatcg tgccagaatg
    20041 tttcccgttt tttagccgat aacgcaatcc catttaccgt tgatgaacag aatggacttt
    20101 ttaccctaac aatcaatcca actggttcgg aatttaatcc caagaaagag gcagaggaat
    20161 actgcgagat cagttttcca cccaaaggta aaaaatacat tgttgttctt accagccatt
    20221 taatgggtaa tggagacaat gatctgggaa aaattctcat gaaaggtttt ttaaacactc
    20281 tacccagtct cgaagggctt ccggaggaga tcatttgtta caacagcgca gttacacttg
    20341 ctgtaaaggg tagcgatact gctcagagcc tagtaaaact aatggaaatg ggtgttaaaa
    20401 tcacactttg cggaacatgt gtcgatttct ttagtataaa ggataaaatt gaggtgggaa
    20461 caatctcaaa tatgctctat attgctgaaa ggcttagctc aggaatccat actgttaaac
    20521 cgtagtaatg gcgaactatt acttcgataa ttcggctacc agtcacccta agccacctga
    20581 agtcgcccaa tacattaatg agtacctgaa gcttggtggc acctatggac gtagtgcata
    20641 cgatagggtt attaatgttt ctcggatagt tgaacatacc cgaatcttac tcgccaggct
    20701 attaggcaca agccttaccg aacatatagt gtttacttat aacgcaaccc atgcactcaa
    20761 tattgccata aagggttttt cctaccggaa aaaacgagta cttatttccc ccttggagca
    20821 taatgcggta acacgccctg tgtttcacct gcagaatact attggacttg agttcgatat
    20881 catgccttac aaccccgatg gttcaataaa tcttgaaggc ctcaggcaat tggatctaaa
    20941 atcatacgat ctgtgtattg ttaaccatgt tagtaatgtg aatggggttg tacagcctat
    21001 tgctgagtta aaagctctgc tgggtgatgt tccgttgctt gtcgatgcaa gtcagtcggc
    21061 agggcatatt cctattgagg tggatacc
//
LOCUS       DAOOWJ010000040        20565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2593_length_20565_cov_7.086494, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000040 DAOOWJ010000000
VERSION     DAOOWJ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 20565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20565
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2593_length_20565_cov_7.086494"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..470)
                     /locus_tag="PLW22_05605"
     tRNA            complement(399..470)
                     /locus_tag="PLW22_05605"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(436..438),aa:Thr,seq:ggt)
     gene            complement(476..558)
                     /locus_tag="PLW22_05610"
     tRNA            complement(476..558)
                     /locus_tag="PLW22_05610"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Tyr,seq:gta)
     gene            811..4083
                     /locus_tag="PLW22_05615"
     CDS             811..4083
                     /locus_tag="PLW22_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOU74215.1"
                     /translation="MKKLLLWVLAIAFTITAVQGQEARLMRFPAVHNNQVVFTYAGDL
                     YTVDISGGVARKLTTHVGYEMFPHFSPDGRTIAFTGQYDGNTEVFSIPAEGGTPMRLT
                     HTATLNRDDISDRMGPNNIVMAWTPDGEKIVYRSRKQSFNDFKGQLFMVPVNGGMSEE
                     LPLSTGGFCSFSPDGKKLAFNRVFREFRTWKYYKGGMADDIWIFDFNTKEVTNITSNP
                     AQDIFPMWIGDEIFFLSDRDRTMNLFVYNTKTKNIEKVTNYNDYDIKFPSFDHNSIVF
                     EKGGFLFAFDVKTRTTRKINITITDDQNYSRPSLVDASKRITNADLSPNGERVVFSAR
                     GDVYTLPSEKGITYNLTASSDVHEREATWSPDGKWIAYLSDKTGEYEVYIQPQDGSMP
                     ATQVTSGINNYIFGIEWSPDSKKILFSDRLGRLQYVDIETKAVTLVTKGKYGLVNSYS
                     WSPDSKWIAYSTSDANRFSVVYIYGIDSKTTNSVTNGWFSSYGPVFSNDGKYLLYVSD
                     REFNPIYSNTEWNHAYVDMSNIYIVMLSKDTPSPFALENNEVKPETKTEQPKDKDSAK
                     KPAKTEEKPKPEVQPVKIDFDGIENRTVELPGKAGRYYNIYCIDGKVYYNYSSSKDRQ
                     PVAMMYDLKKQKETELGESIRFTISSNGKKMLVSKNRTYAVIDLPTGKINMEKTIDLS
                     DMKVWVDYHKEWKQIFDESWRQMRDFFYVENMHGVDWKAIHDKYAVLVPYVNHRNDLT
                     YIIGEMIGELNVGHAYVNSGDKPEPERIKTGLLGAKLSKHSSGYFRVDKILEGANWSD
                     ALRSPLAEIGVNVKEGDYIISVDGVDLKNIPDIYSTLINKADKTVALVVNSSPQANGG
                     RKVLVKPIADESELYYYTWVQNNIRKVNEATNGEVGYIHIPDMGVAGLNEFVKYFYPQ
                     LTKKALIIDDRGNGGGNVSPMILERLSRVVYRMTMRRGFDQPSTIPSETHYGPKVVLI
                     DRYSASDGDLFPYGFKKLGLGPLIGVRSWGGVVGISGSLPFVDGGDLRKPEFTSFSSD
                     TGEWIIEGHGVDPDIEIDNDPYREYMGQDDQLNKAIEQIKVMLKDYKPLPQIPQAPDR
                     SK"
     gene            complement(4128..4307)
                     /locus_tag="PLW22_05620"
     CDS             complement(4128..4307)
                     /locus_tag="PLW22_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU74216.1"
                     /translation="MDANEKVIQTLKSAGKPMKAGEIAEAAGVDKKEVEKAIKKLVAD
                     GKLHSPQRCFYDIKK"
     gene            complement(4332..5228)
                     /gene="xerD"
                     /locus_tag="PLW22_05625"
     CDS             complement(4332..5228)
                     /gene="xerD"
                     /locus_tag="PLW22_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOU74217.1"
                     /translation="MGWSNAIDDYINFLRLEKSLSENSIAAYRTDMDKLWKFAEGKGV
                     EPESIRRNDLEEFLAQLHDLGLNKRSQSRILSGVRGFFKYLLLEEVIDTDPTELIDSP
                     KIGRKLPEVLSVAEIDALEAAIDLSKPDGHRNKAIIETLYSCGLRVSELVSLRLTDLF
                     FTDGFIRVIGKGDKERLVPIGTKAINDINAYLAQRNSIDSRIDPDSRNIVFLNRWGRK
                     LTREMVFTIIKKYASLAGIKKNISPHTLRHSFATHLIEGGADLRAVQEMLGHESIQTT
                     EIYTHLDNQYLRETIMMFHPHK"
     gene            5364..5939
                     /locus_tag="PLW22_05630"
     CDS             5364..5939
                     /locus_tag="PLW22_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74218.1"
                     /translation="MVNSDLVATGLIAAYTLYSLVAIGLFGWFAYRITKPTKTPVIKP
                     VLFYSFTTLLVIIGVSLHLTTMHTIPWVKLEMDASKINPDRLIKMEVDSFKFYILTDS
                     IRQELPVAEPIKIKEGETVMFDVYSKDLTYGFGLFRPNNTMVLQMQVLPLYSNKIIWE
                     FNEKGTFTLRSTEFAGPKSTRMIIPNFITVE"
     gene            5955..7592
                     /locus_tag="PLW22_05635"
     CDS             5955..7592
                     /locus_tag="PLW22_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOU74219.1"
                     /translation="MKKFIDTLINGNEVNGRLAGLTPLQKLTLRFAVVSLVYYGFAVI
                     EGMIMRLILASPNSLTELIPEHQYFAILTAHPLVGIFGATYTLVFGAFYFALPFLLKK
                     PLWGFKAANWSFWLITIGVFVFWFAGFLSHYGPLYTLYWPLPADFNQFGPWGGTFFIL
                     GIALVMVASIIFVVIVFKTITYTPKGWEKQPGGSLLASALGVSGLANLFRKKENRKHH
                     QVPLPVAAIARGSVDVFLNAGIITFTGVLILVYLLGHILGFNLQNSWIDALLYKNMFW
                     WGLDLIADGLVLIFVAGTWYLLAMLITGVKNVYMENVARALLLLELVVSWTVWSHHLL
                     SDQAQPLMLKLVSGQFVTAFELITQGLALFISLVTLWGARPLKWTPELKFFLGGLLGF
                     ALAVAAGIIQADMGMNRILHNTQWIVGPHVHVAVLIGLTMTLYAVVYKLLPVLTNGLN
                     IFSVKLTSWHFWLHLLGGIGMGAFMGMAGLKGMLRRTIYYNGEFDIFMILAALAGAAL
                     LLAYLSYFFNLVLTIGVKGIIEIFKPSKLPKEQILPE"
     gene            7699..9114
                     /gene="pyk"
                     /locus_tag="PLW22_05640"
     CDS             7699..9114
                     /gene="pyk"
                     /locus_tag="PLW22_05640"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOU74220.1"
                     /translation="MLKRTKIVATISDKRCDVDFIKSLYQAGMNVARLNTAHMNPESA
                     KNLISNIRSVSENIAILVDTKGPEIRTSSNGEEIKVETGMKIKIIGNPDGASGNDTVY
                     VSYPNIAAEVPVGNTILIDDGEIELKVVDKKENALYCEVTNCGTIKLRKSVNIPNVHI
                     NLPSLTEKDKAFVQFAIENNIDFIAHSFVRNKHDVLEIKEILHKHNSPIKIIAKIENQ
                     QGVDNIDEILDHAYGVMVARGDLGIEIPAEKLPIIQRRIVRKCIESKKPVIIATQMLH
                     TMIEHPRPTRAEISDIANAIYQRTDAIMLSGETAYGQYPVEAVEVMTRVAREVEEAQE
                     ADLSVNLVRINNEVTATLAKAAVRACSSLPIKAIIVDTLTGRTGRYLAAFRGRIPIFA
                     ICYKQHVMRELALSYGVEAVYMEPRTSRDHFLTDAIGTMLERRKIASEDMVLIIGGSF
                     GPSNGASFMEISKVKNLVSKK"
     gene            9118..10347
                     /locus_tag="PLW22_05645"
     CDS             9118..10347
                     /locus_tag="PLW22_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74221.1"
                     /translation="MPDSLGKSVKLHIAILAIQLICQLGYSQKMPNAYYRAEAALELN
                     DTKNALSWADSCFNAKPYRYQYYLVKGKVLLQANRYNEAIEAFLQAEQQRSGIASYQL
                     ARTYCLMGDTINCIEWTRQNLQSIYREPESSFLLSNDFSFAHSLKEWQKIWDKEWYSA
                     IEKDIHYARYLIGNQNYDEAIELLNKRIKSKKSKAELFELRGRAHLYAGNFSLALRDF
                     ENAYHRSKRNYDYLALQANALFNLNQNGKALTQIEQAIDKSGGKPEYLLLKAKILSKL
                     NRYSEAYETLKEYLVYFPNNTEANLNLARYAYESGYYTDALLAIAKMLKFTPDNIELI
                     LLRGKIYLKTDQPRQAIFDFEKAISQNYCTAESYLLKGLALLQVGEREEACKCFSISG
                     GLGNIDAQGLHFNTCRK"
     gene            10442..10999
                     /locus_tag="PLW22_05650"
     CDS             10442..10999
                     /locus_tag="PLW22_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74222.1"
                     /translation="MEIKGSAVKATPDFVKANFINRYSEWLKSLPSSSREIIEKPIYA
                     TTWYPLIESVIIPTQKVADLFYNGDYNKAAREIGRFSADIALKGIYKIFVRISSPQFV
                     LSRASSIFSTYYQPADIKVVESSDKKAVLQLEKFHVDEKLIMERIAGWIEHTLEITLK
                     SPLKVDVEQIVNGKNLTARITAMWQ"
     gene            11059..11826
                     /locus_tag="PLW22_05655"
     CDS             11059..11826
                     /locus_tag="PLW22_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOU74223.1"
                     /translation="MLGLAAKSLVPVRKEPNETTEMVNQLLFGEKVTILENYKQWYRI
                     ESTLDSYPGWVDSRLIDIVADTSSEKELTNFSVLSSLAEAIKVDDNSRYLLSPGSILY
                     GYSGKKFHLNGSNFELTANIPSLPTNPLDHIIKTAQAFINTPYLWGGKSIFGIDCSGL
                     VQVCFRTAGVFLPRDAWQQVQNGQTVEFVDHAQPCDIAFFDNEEGRIIHVGILINPNE
                     IVHSSGYVRIDKFDHQGIYNPQLGIYTHKLRIIKRVL"
     gene            12121..12573
                     /locus_tag="PLW22_05660"
     CDS             12121..12573
                     /locus_tag="PLW22_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU74224.1"
                     /translation="MYTYEYPRMLVTVDAVIFKVDINYKPTHLLLIERGNEPYKGHFA
                     LPGGFPEIKELLVDAAARELFEETGIAGVELKQIGAFDDIDRDPRDRNIAIAFWGVVS
                     DDKISPTAGDDAAKAQWFPLDNLPPLAFDHSKIVKAALGCLKRGFPEQ"
     gene            complement(12579..13856)
                     /locus_tag="PLW22_05665"
     CDS             complement(12579..13856)
                     /locus_tag="PLW22_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU74225.1"
                     /translation="MEDKVIERSYLDMELLRFTTAGSVDDGKSTLIGRLLYDSKAIFQ
                     DQLEAIERASIKKGEEYINLALLTDGLRAEREQGITIDVAYRYFHTPKRKFIIADTPG
                     HVQYTRNMVTGASTANAAVILVDARHGVIEQTIRHAYIASLLRIPHVIVCVNKMDLVD
                     FKQDVFDNIQQKFHEFATHLDLHDVRFIPISALLGDNVVDRSKNMDWYQGPTLMYLLE
                     NLYISNDYNQLDARFPVQYVIRPQSIEFHDYRGYAGRMASGTFRVGDRVKVLPSGFTT
                     TIKGIDFWTDHLDSITAPQSGTIILEDDLDVSRGCMIIPEENGIKQGQDIELMICWLG
                     DKPMVPGGKYALKHTTNDLRCMVKEVKYKININTLEKDYNDKSVGMNDIACITIRTTK
                     PLFYDSYRQNRVTGSLILIDEATNVTVGAGMIV"
     gene            complement(13875..14783)
                     /gene="cysD"
                     /locus_tag="PLW22_05670"
     CDS             complement(13875..14783)
                     /gene="cysD"
                     /locus_tag="PLW22_05670"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOU74226.1"
                     /translation="MSNKVPINHLRELESEGVFVIREVVSQFENPVMLFSGGKDSIVM
                     FHLARKAFYPAKVPFPLMHIDTGHNFQETLDFRDWLVQETGVQLIVRYVQDSIDKGRV
                     VEETGYNASRNKLQTVTLLDAIEELKIDCAMGGGRRDEEKARAKERFFSHRDEFGQWD
                     PKNQRPELWNLFNGRKNMGEHFRVFPISNWTEMDVWQYILMENIAIPSLYFSHEREVF
                     DRDGVLYAKTPFLKMKDTEKAEKRIVRFRTIGDATCTGAIESVASSVEDIIREVAATR
                     VTERGGRADDKRSEAAMEDRKIEGYF"
     gene            complement(14969..15505)
                     /gene="cysC"
                     /locus_tag="PLW22_05675"
     CDS             complement(14969..15505)
                     /gene="cysC"
                     /locus_tag="PLW22_05675"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOU74227.1"
                     /translation="MSYKHPITVWITGLPASGKTTLGKALQGRINQLKIPVVLLDGDE
                     VRKGLCSDLGFSPADRTENIRRVANMAALLNSQGVVTICCFVSPLISMREMARSIVGS
                     KNFFEVFTDAPVEVCQSRDIKGNYSKAINGSLPNFTGISDSYEAPMNPSLRLNTAELS
                     EGEAADILYDHFLRWVDK"
     gene            complement(15511..16152)
                     /locus_tag="PLW22_05680"
     CDS             complement(15511..16152)
                     /locus_tag="PLW22_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOU74228.1"
                     /translation="MKQILSLLFISSSLTLFSQEVNPDGIVRSFSNKMQEIKSLSATF
                     SFTLENLQERITDTHQGKILAKGKMFYLDLMGMEVFSDGQTKWQYIKEAKEVTISKMN
                     IKENSFLDDPMKLFKDYDKNFKYKYIGQQKSKNRVLHEVDFFPRDLNLPYSSVKLQFD
                     ANTLEPYLIRYQGKDGNNYIIQIKNFKSNVPLRDDRFKFDVEKHKGVEVIDMR"
     gene            complement(16194..>17753)
                     /locus_tag="PLW22_05685"
     CDS             complement(16194..>17753)
                     /locus_tag="PLW22_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOU74229.1"
                     /translation="ERNEEKELAISDINEQELYDPTLELSSYQKPLIELLEDYKNTES
                     PVTNEMLVENKNKIVETLSHYKIQIDKIKATIGPTVTLYEIVPAPGVRISKIKSLEDD
                     IALSLSALGIRIIAPIPGKGTIGIEVPNQNPEVVSMHSIIKSQKFQECKYDLAIALGK
                     TISNEIFMLDLTKLPHLLVAGATGQGKSVGLNAIITSLLYKKHPAEIKFVLVDPKMVE
                     LTLYNKLERHFLAKLPDSPEAIITDTQKVIYTLNSLSVEMEDRYKLLNLAKVRTIKEY
                     NQKFVARRLNPNNGHRYLPYIVVIIDEFADLIMTAGREIEMPLGRLAQKARAIGIHLI
                     IATQRPSTNIITGVIKANFPARIAFRVSSMVDSRTILDTPGANNLIGRGDMLVSAGSD
                     LVRVQCAFVDIPEIERITDFIGNQPGYPHAYELPEYVPEGQDGPVDVDLRKRDELFED
                     AARLVVQTQSGSTSLIQRKFSIGYNRAGRIMDQLEAAGIVGPADGGKPRQVLIVDEVS
                     LERILNTLSVD"
     assembly_gap    17754..17853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17854..18478)
                     /locus_tag="PLW22_05690"
     CDS             complement(<17854..18478)
                     /locus_tag="PLW22_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOU74230.1"
                     /translation="MARKDKGTDVEDSDSKDVSNDSLFKDERIRFTVGLFLILLGVYI
                     FLSFLSFLFTWKVDQSIEWVSIFSSTEQKIGNWGGKLGASLSNQFINHWFGITALAFP
                     LIFMVLGLKLLKFIRGRVTKFIFKVLLGILVGSLTLGFLFKELGGYLGSGLGGAHGLF
                     VSEWLSTVIGKVGAAFLVLIALIAYIIYVMPKSLYHFQSGTFFLFSSL"
     gene            complement(18538..19233)
                     /locus_tag="PLW22_05695"
     CDS             complement(18538..19233)
                     /locus_tag="PLW22_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74231.1"
                     /translation="MNRSVYQLVGVAVILFSLSSCISKSPKEISEGRIDYDIEYDTLT
                     LSRIDKKILPSSLVVRFCNNNTINTIDGLSGAITISIISQPSKKEFTTLLKVFNKKLY
                     HSEPYTNGHYPALYARIPKVNIDTLVRDCDYIGYKCKSVMGYFADNPDNKFEIIYTKQ
                     IAIDNPNLNTPFENIDGVMLGFNLRFNNLEMRLRARNVAKENINNDAFKIPKDYKKVD
                     FQTMTDLIYLLQQ"
     gene            19463..20422
                     /gene="arcC"
                     /locus_tag="PLW22_05700"
     CDS             19463..20422
                     /gene="arcC"
                     /locus_tag="PLW22_05700"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOU74232.1"
                     /translation="MSKLAVVALGGNALLRGNQVGTIDEQEQNTTDTLENLVYLIKEG
                     YNLVISHGNGPQVGNILMRNDAGEQLYGIPQMPLDICVADSQGVIGYMIERMLRNVLR
                     KHGIDREVCCLVTPVIVDHDDPAFTNPTKRVGRIYDKAQADALAAEKGWIFKEEVKDT
                     GSGWRRVVPSPKPKGILNIKVIDQLVRQGIIVIASGGGGVPVYIDEEKNVRPAEAVID
                     KDLASALMAAEIKADEFYILTDVPFVYLNYKKPNEQKLEFLNYADTMKYLEQGMFGEG
                     NMAPKIKACLDFIKRGGKMSVITEATKLEDMSYGTKITMEYED"
     gene            20450..>20565
                     /locus_tag="PLW22_05705"
     CDS             20450..>20565
                     /locus_tag="PLW22_05705"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HOU74233.1"
                     /translation="MSFNLRNRSFLKLLDFTPKEIQFLLDLSADLKKAKYAGT"
BASE COUNT         6078 a         4513 c         4233 g         5641 t
ORIGIN      
        1 tcaggataac cgatgagaaa gagcttggca gggtaaagcg gtagctttta gcgcatgtaa
       61 ccgatgcgtt tgatgaattt aataaacatg tttaaaaggg gaggaaaatg gataaatcca
      121 ttaatacgat accacgaatc caaccatagc ccggggttta aaccccgggc tattgaacgg
      181 attaaataat aaaaaccctt ttaacgtaaa aaaatgggga aaatggataa atctattcaa
      241 ataatatctc gaaattccac tagcccacca attaattcgt gggctattgg aaatgatttt
      301 ttttaaacaa attttttatc ctcaactagc cgagtatttc cataaacaaa aaaccgccca
      361 tagaggcggt tctttaattt gctaactttt tggctttgag ccgatgacga gaattgaact
      421 cgtgacccct tccttaccaa ggaagtgctc tacccctgag ctacatcggc aagtgtgggg
      481 agagcaggat tcgaacctac gaaggcataa gccagcagat ttacagtctg ccccagttgg
      541 ccactttggt atctcccctt ccctctcaaa aatcgcgttg caaaagtagt aattttagca
      601 aaactcgcaa gcgccctttg ctattggcct tgaaaaaatt tacgtcgaaa ggcgaagggg
      661 ttgaaaatca acactaacat caacaaacct agtaatccat tacatggttt tataggtgta
      721 ttcttattat ttaactgagg ggataaactt tttttcatga aataaatcta acttacacta
      781 aaatcaactt tacaactaaa acctataacc atgaaaaaac tgttactttg ggtattggct
      841 atagccttta ccatcacagc agttcagggg caggaggcac gcttaatgcg tttcccggct
      901 gtgcataaca atcaggtggt gtttacctat gccggcgacc tatacactgt tgacataagc
      961 ggaggcgttg cccgcaagct aaccacccat gttgggtatg agatgtttcc tcacttttcg
     1021 cccgatggta gaaccattgc ctttaccggg caatacgatg gaaacactga ggttttcagc
     1081 attcctgctg agggaggaac tcccatgcgt ttaacccata cggctaccct aaaccgcgat
     1141 gatatctcgg accgaatggg gccaaacaat atagtaatgg cctggacgcc cgatggcgaa
     1201 aagattgttt accgttcgcg caagcagagc tttaacgatt ttaaggggca actattcatg
     1261 gttcccgtca acggcggcat gagcgaggaa ctaccccttt ccaccggtgg cttttgctct
     1321 ttctcgcccg atgggaaaaa gcttgccttc aacagggttt tccgtgagtt ccgtacatgg
     1381 aaatactaca agggtggcat ggcagatgat atctggattt ttgactttaa caccaaagag
     1441 gttaccaaca tcaccagtaa ccccgcacag gacattttcc ccatgtggat tggcgatgaa
     1501 atcttcttcc tatcggaccg cgacagaacc atgaaccttt ttgtgtacaa cacaaaaacc
     1561 aagaacatcg aaaaggttac caattacaac gattacgata tcaagttccc ctcgttcgac
     1621 cataattcca ttgtttttga gaagggcgga ttcctatttg cctttgatgt aaaaacgcgt
     1681 acaacccgga aaataaacat caccataacc gacgaccaga actactcacg cccctcgctg
     1741 gttgatgcaa gcaagcgcat caccaatgcc gacctctcgc ccaatggcga acgggtagtt
     1801 tttagtgcac gtggcgatgt ttacactcta ccatcggaaa agggtattac ttacaaccta
     1861 actgcttcat ccgatgtcca tgagcgcgaa gccacttggt cgcccgatgg taaatggatt
     1921 gcctacctct ccgataaaac aggagagtat gaggtttaca tccaacccca ggacggtagc
     1981 atgcccgcca ctcaggtaac ctcgggcatt aacaactaca tttttggcat agagtggtcg
     2041 ccggatagta aaaaaatact ttttagcgat cgcttgggta ggctacagta tgttgatata
     2101 gaaactaaag ccgttacctt ggttactaag ggtaagtatg gcttggtaaa cagctatagc
     2161 tggtcgcccg atagcaaatg gattgcctac tctacctccg atgccaaccg attctctgtg
     2221 gtttacatct acggcattga cagtaagacc accaattccg ttaccaatgg ctggttctca
     2281 tcgtatgggc cggtattttc aaacgatgga aagtacctgc tttacgtatc ggatagggag
     2341 tttaatccca tatacagcaa cacggagtgg aatcacgcct atgttgacat gagcaacata
     2401 tacatagtga tgctctccaa ggatacaccc tcaccctttg cccttgaaaa taacgaggta
     2461 aaacccgaaa ccaaaacaga gcagcccaag gacaaggatt cagccaaaaa accagctaaa
     2521 accgaggaga aaccaaagcc tgaagttcag cctgttaaaa tcgattttga cggcattgag
     2581 aatcgcaccg ttgagttacc gggcaaagca ggccgatact acaacatcta ctgtattgat
     2641 ggcaaggttt actacaacta ctccagctcc aaggatcgcc aacctgtggc catgatgtac
     2701 gacctgaaga aacaaaagga gactgaactg ggcgaatcca tcaggttcac catttcatcg
     2761 aacgggaaaa agatgctggt atcgaaaaac cgcacctatg cagtaatcga cctgccaacc
     2821 gggaaaatca acatggaaaa gaccattgat ttaagtgaca tgaaggtttg ggttgactac
     2881 cacaaggagt ggaaacaaat attcgacgag agctggcgtc agatgcgcga tttcttctat
     2941 gtagagaaca tgcatggtgt tgattggaaa gccattcacg ataaatacgc tgtactggta
     3001 ccctacgtta accaccgcaa cgacctaacc tatatcattg gcgaaatgat tggcgagctg
     3061 aatgttggtc acgcttacgt gaacagtggc gataagccag aacccgaacg cattaaaacc
     3121 gggctgttag gagctaagct gagcaagcac agcagcggct acttcagggt tgacaaaata
     3181 cttgaaggcg ccaactggag cgatgcgctc cgttcaccac tggccgagat tggtgtgaat
     3241 gttaaggagg gcgactatat aatttcagtg gatggggttg acctcaaaaa catccccgat
     3301 atctactcta cccttattaa taaggccgat aaaacagttg ctcttgttgt taacagctca
     3361 ccacaggcta acggaggccg caaggtgctt gtaaaaccca ttgccgacga atcggaactt
     3421 tactactata cctgggttca gaacaatatt cgcaaggtaa acgaagccac caatggcgaa
     3481 gtgggctaca tccatattcc cgatatgggt gttgccgggc tcaatgagtt tgtgaaatac
     3541 ttctaccctc agctcaccaa aaaggcttta ataattgacg acagaggtaa tggtggtggt
     3601 aatgtatcgc caatgatact tgaacgccta agccgtgtgg tttaccgtat gaccatgcgc
     3661 cgtggattcg atcaaccatc aacaatccct tcggaaacgc attacgggcc aaaggttgtg
     3721 ctcattgacc gttattccgc ctccgacggc gaccttttcc cttacggttt caaaaagtta
     3781 ggccttgggc cacttattgg cgttaggagc tggggcggcg ttgttggtat ttcaggctca
     3841 ctaccatttg ttgatggtgg cgacctgcgt aaaccagaat ttaccagctt tagctccgat
     3901 acgggtgagt ggataattga aggacatggc gttgatcccg atattgaaat tgacaacgac
     3961 ccttacaggg agtacatggg gcaggatgat cagctgaata aggctattga gcagattaag
     4021 gttatgctta aggattacaa gcctctaccg caaatacccc aagcacctga caggtcgaag
     4081 taatgaaata aaaaaacggg ttgaagtttt caacccgttt tttttattta ctttttgata
     4141 tcgtaaaaac agcgttgggg agagtgaagc ttcccatcag ccaccagttt tttgattgcc
     4201 ttttcaacct ctttcttatc aacacctgca gcttcggcta tctcacctgc tttcatcggc
     4261 ttaccagccg attttagggt ttgaattact ttctcatttg cgtccatagt aaactcattt
     4321 tagtttgaca tttatttatg gggatgaaac atcattattg tttcccttag gtactggtta
     4381 tcgagatgtg tataaatctc ggtggtttga atcgattcat gcccaagcat ctcctgaacg
     4441 gcgcgcaaat cggctcctcc ctcaattaaa tgggtggcaa aggaatgcct gagcgtatgg
     4501 gggcttatgt tctttttaat cccggccagt gaagcatact ttttaataat ggtgaacacc
     4561 atctcgcggg ttagctttcg gccccatcgg tttaggaaaa cgatattgcg cgaatcagga
     4621 tcaatacgtg aatcgatgct gttacgctgc gcaaggtatg cattgatgtc gttaatggct
     4681 tttgtgccaa tgggcacaag cctttcctta tcgcctttac caataaccct aataaagcca
     4741 tcggtaaaga acaaatcggt aagcctaagc gacactaact ccgacacacg aagcccacag
     4801 ctgtaaagcg tttcaataat ggctttgttt ctgtgcccat cgggtttgct aaggtcgatg
     4861 gccgcctcta aagcatcgat ttcagctacg cttagaactt cgggcaattt tcggcctatt
     4921 ttgggagaat cgatcagttc ggtaggatcg gtgtcaatca cctcctcaag tagcaggtac
     4981 ttaaaaaagc ctctaactcc tgaaagaatc ctggattgcg aacgcttatt caagcctaaa
     5041 tcgtgaagct gagccaggaa ctcctccaaa tcgttacgcc gtatcgattc aggctcaacg
     5101 cctttcccct cggcaaactt ccagagctta tccatatcgg tgcggtaagc tgcaatggag
     5161 ttctcggaaa gcgatttctc aagtcgcaga aagttaatgt aatcgtctat tgcgttgctc
     5221 catcccattt taactcgtta ggtttataca aatatatgca ataacccata aatgattaga
     5281 atcagcctat ggttgtttta tatcaacttt aacctacgtt aaaatttcgt aaattttgca
     5341 tgtaaaccaa aacaaacaaa actatggtaa actccgattt agttgcaaca ggactaattg
     5401 ctgcttacac cctttatagt ttggtagcaa ttggtctatt tggttggttt gcctaccgaa
     5461 taaccaaacc aactaaaacg cctgtaataa agcctgtact attttactct tttactacgt
     5521 tgcttgtaat aattggcgta tcgctccacc tcactaccat gcacaccatt ccttgggtaa
     5581 aactggaaat ggatgcatca aaaatcaacc ctgacaggtt gattaaaatg gaggtcgata
     5641 gttttaagtt ttacatcctt accgattcaa ttaggcagga gttgcccgtt gctgagccta
     5701 ttaaaatcaa ggagggtgaa acagttatgt tcgatgtgta ttccaaagac ctaacctatg
     5761 gatttggatt attcaggcct aacaatacta tggtattaca aatgcaggta ctaccacttt
     5821 acagtaacaa aatcatatgg gagttcaatg aaaaggggac tttcaccttg cgttcaaccg
     5881 aatttgcagg cccaaaaagc acccgtatga taatacctaa ctttattact gtcgaataat
     5941 aaaattgttg gtctatgaaa aagtttatag atactttaat aaatggcaat gaggtaaacg
     6001 gacgcttagc aggccttacc ccattacaaa aattaacctt aaggtttgct gtagtatcac
     6061 ttgtttacta tggctttgct gtaattgaag ggatgataat gcgcttaatc cttgcatcac
     6121 caaactcact cactgaacta attcctgaac atcaatactt tgcaatccta actgcacatc
     6181 cacttgttgg tatctttggt gccacctaca cccttgtgtt tggcgccttc tattttgcgt
     6241 tacctttcct tcttaaaaaa ccattatggg gatttaaagc tgcaaattgg tcgttttggc
     6301 tgattactat tggggttttc gttttctggt ttgcaggatt cctttcgcat tatgggcctc
     6361 tatataccct atactggcca ctaccagctg actttaatca atttgggcca tggggtggca
     6421 cattctttat tcttggaatt gccttggtaa tggtggcctc cataattttt gttgttattg
     6481 tttttaaaac aattacttac acccctaaag ggtgggaaaa gcaaccgggt ggttcacttt
     6541 tggcttcggc actgggagta tcggggctag caaacctttt ccgaaaaaaa gaaaatcgta
     6601 aacatcacca agtaccatta ccagttgctg ccattgctag gggatcggtt gatgtattcc
     6661 tgaatgctgg tataataacc tttacgggag tgcttattct ggtttactta ttaggacata
     6721 ttttgggatt taacctacaa aactcatgga ttgatgcgct cctctacaag aacatgttct
     6781 ggtgggggct cgacctaata gctgatggtt tagttctcat ttttgttgcc ggtacttggt
     6841 acctacttgc catgctaata actggcgtta aaaatgtata catggagaac gtagctaggg
     6901 cactactttt gctcgaacta gtagtttcct ggacagtttg gtcgcaccac ctcctttccg
     6961 atcaggcgca gcccttaatg ctaaaacttg tgtctggtca atttgtaaca gcgtttgagc
     7021 ttataaccca aggcttagct ctatttataa gtttagtaac tctttggggt gcacgccctc
     7081 taaaatggac accagaacta aaatttttcc ttggaggttt acttggtttt gccctagctg
     7141 ttgcagcagg aattatccaa gccgatatgg ggatgaatcg tatactacac aacacacaat
     7201 ggattgttgg gcctcacgtc cacgttgctg ttctgattgg acttaccatg accctatatg
     7261 cagtagttta taaattacta cccgtactaa ctaacggttt gaacattttt agcgtaaagc
     7321 ttacatcatg gcatttctgg ctacatctac ttggaggtat aggtatgggt gcgttcatgg
     7381 gcatggctgg gcttaaaggc atgctccgca gaaccatcta ctacaatggc gagtttgata
     7441 ttttcatgat tcttgcagca ttagctggag cagcattact tttagcctac ttgtcatact
     7501 ttttcaacct tgtcctcaca atcggagtaa aaggaattat tgaaatcttc aaaccatcaa
     7561 aactacctaa ggagcaaata cttcccgaat aatttactta acgtaatacg caaaagggct
     7621 ttcgaatttt cgattgccct tttctttttt tagaaagctt aacaaaaact tgagtatttt
     7681 tgcaaaaaaa atccacctat gctcaaacgt acaaaaatag ttgccaccat atccgataag
     7741 cgttgcgatg tagattttat aaaatccctt taccaggctg gaatgaacgt tgcacgtttg
     7801 aacaccgccc acatgaaccc agaatcagct aaaaatctga tttcaaatat ccggagtgtt
     7861 tctgaaaaca tcgctatact tgtcgatact aaaggccccg agattagaac ctcatcaaat
     7921 ggtgaggaaa ttaaggtgga aaccggaatg aaaataaaga ttattggcaa ccccgatgga
     7981 gcctcaggaa acgacaccgt gtacgttagc tacccgaaca ttgcggccga agtaccagtt
     8041 ggtaacacca ttcttataga tgatggcgaa attgagctaa aagtggttga taaaaaggaa
     8101 aatgccctct actgcgaggt aacaaactgt ggtaccataa aactgcggaa aagtgtaaat
     8161 ataccgaatg tgcatatcaa ccttccctcg ctaaccgaaa aggataaggc gtttgttcag
     8221 tttgccattg aaaacaatat agatttcatt gctcacagct ttgttcgtaa caaacacgat
     8281 gtacttgaaa tcaaggagat actccataag cacaatagcc caattaaaat tattgccaaa
     8341 attgagaacc aacaaggtgt tgataatatt gacgaaatcc tcgaccacgc ctacggagta
     8401 atggtggctc gtggtgattt aggtatagag attcctgccg agaaactgcc aattatacaa
     8461 cgccgaattg ttaggaaatg tatagagagt aaaaagccgg taataattgc aacccagatg
     8521 ctgcatacca tgattgagca tccgcgtccc acgcgtgctg aaatcagcga tattgccaat
     8581 gccatatacc aacgtaccga tgccattatg ctaagcggcg aaacggcata cggtcagtac
     8641 cccgttgaag cggttgaggt gatgacccgc gttgcacgcg aggttgagga agcccaagag
     8701 gctgaccttt cagtaaacct tgttcgaatt aacaacgagg taacagcaac actggctaaa
     8761 gctgccgtta gggcatgttc atcgcttccc atcaaagcta ttattgttga tacacttaca
     8821 ggtcgcaccg gccgatacct tgctgcattc cgtggtcgaa ttcccatatt tgccatttgc
     8881 tacaagcaac atgtaatgcg cgagttagca ctatcgtatg gcgtagaagc agtttacatg
     8941 gagccacgca cctcacgcga tcatttccta accgatgcca ttggcaccat gctcgaacgc
     9001 cgaaaaatag cctccgagga catggtactc atcattggag gtagctttgg gcccagcaat
     9061 ggtgcttcgt tcatggaaat tagcaaggta aaaaatctgg tatccaaaaa gtaaaaaatg
     9121 cccgattctt taggcaaatc agtaaaactg cacatagcaa ttctagctat tcaacttatt
     9181 tgccaactgg gctactccca gaaaatgcca aatgcttact acagagcaga ggcagcgctt
     9241 gaacttaacg acacaaagaa tgccctcagc tgggctgatt cgtgcttcaa tgccaaacca
     9301 taccgctacc agtattacct tgtaaaaggg aaagtccttt tacaggccaa tcggtataat
     9361 gaggccattg aggccttttt acaggctgaa caacaacgct caggcattgc atcgtatcaa
     9421 ctggctagaa catattgcct catgggcgat acaataaact gtatagaatg gactcgtcag
     9481 aatttacagt caatatacag agaacctgaa agtagcttcc tactcagtaa cgacttttca
     9541 tttgcgcatt ccttaaagga atggcaaaaa atctgggaca aagaatggta ttccgcaatt
     9601 gaaaaggata ttcactatgc ccgttacctg atcggtaacc aaaactacga cgaggctatt
     9661 gaactcctta acaaaaggat aaaaagtaaa aaatcaaaag cagaactatt tgagttacgg
     9721 ggacgtgcgc acctttatgc cgggaacttt tcacttgcac taagagattt tgagaacgca
     9781 taccatcgca gcaaaaggaa ttatgattac ttggcactgc aggccaatgc actatttaac
     9841 cttaatcaaa acggaaaagc gttaacgcaa atcgagcaag ctattgacaa atcagggggt
     9901 aaacccgaat acttgctgtt gaaggcaaaa atcctcagta aattaaaccg ctatagcgag
     9961 gcgtatgaaa ccctaaagga ataccttgtg tatttcccca acaacaccga agcaaattta
    10021 aacctagcaa ggtatgctta tgagtctgga tactataccg atgccctact ggcaatagcc
    10081 aaaatgctca agtttactcc cgataacata gaacttattc tgcttagggg aaaaatctac
    10141 ctaaaaaccg accaaccccg acaagcaatt ttcgactttg aaaaagcaat cagccagaat
    10201 tattgcactg cagagtcgta cctgctaaaa ggtttagctc tgctgcaagt aggcgaaagg
    10261 gaagaagctt gtaaatgctt ctcaatatct ggcggtttgg gtaacatcga tgctcagggt
    10321 ttgcatttca acacctgcag aaaataataa ctataaacat tcatcatcaa tcaaagaatt
    10381 aacgtcaaaa aaaattgata ataatcaaaa gtttgtttaa aattgtgtaa aatgaaaaaa
    10441 catggaaata aaaggttcag cagtaaaagc aacccctgac tttgtaaaag ccaactttat
    10501 taaccgctac tcggaatggc taaagtcact accaagctca tcgcgtgaga ttattgaaaa
    10561 acctatttat gcaacaacat ggtacccgtt aattgaatcg gtaatcatac ctacgcaaaa
    10621 agtagccgat ttgttttaca atggcgatta caataaggca gcacgtgaaa ttggaaggtt
    10681 tagcgccgat atagccctca aaggcatata taaaatattt gttaggataa gttcaccaca
    10741 gtttgtatta agtagagcat caagtatatt ttccacttac taccaacctg ccgacattaa
    10801 agtggtggaa agttccgata aaaaagcagt acttcagctt gaaaaattcc atgtcgacga
    10861 aaaactcata atggagcgaa ttgcaggctg gattgagcat acccttgaaa tcacacttaa
    10921 atcacccttg aaggtcgatg tggaacaaat tgtaaacggg aagaacctta ccgctcggat
    10981 aactgccatg tggcaatagc tacaatagtt attgaatgaa acctgataat tgctatattt
    11041 acaaaaaaaa tagcaattat gctgggattg gcagctaaaa gtttggttcc ggttcgcaag
    11101 gaacccaatg aaaccactga gatggttaac caactacttt ttggcgaaaa ggttacaatt
    11161 cttgagaatt ataagcaatg gtatagaatt gaaagcaccc ttgacagcta cccgggatgg
    11221 gtggactcca ggctaattga tattgtagca gacactagca gcgaaaagga acttaccaac
    11281 ttttcggttc tctcatcact ggccgaggcc ataaaggtag atgataactc aaggtattta
    11341 cttagtccgg gatcgattct ttacggatat tctggaaaaa aattccactt aaacggctca
    11401 aattttgaac ttactgccaa tattccctct ctgcccacta atcctttgga tcatataatc
    11461 aaaaccgccc aagcatttat aaacaccccg tacctatggg gcggcaaatc gattttcggc
    11521 atcgattgtt ctggcctggt tcaggtttgc ttcagaactg ccggggtatt cctcccacgc
    11581 gatgcgtggc aacaggttca aaacggccaa actgttgagt ttgttgacca tgcccaaccc
    11641 tgcgatattg ctttcttcga taacgaagag ggacgtataa tccatgttgg tatactaatc
    11701 aatcccaatg aaatagtaca tagctccggt tacgttcgaa ttgataaatt tgaccatcaa
    11761 ggaatttaca acccgcaact gggcatatac acccacaagc ttaggattat taagcgggta
    11821 ctataagcca taatggaacg gaagacaatg gcttggtggg ccaaacgaaa gaaatctaaa
    11881 agaatgtaat ccaattgatt ccaatgcatt ctctatgcaa gtctgcataa ttatagtttt
    11941 gcttgacatt aaaacatatt tttggtatct ttcaaaatcc gttctaaact caaccgtatg
    12001 ctaaaacacc aacttttgat agccattgcc attattgcat ttgcaatttg ggttgctctt
    12061 agaaaacgcg atcaaaaaaa atcggacaac gaagaggacc ctgataatga atagtttagc
    12121 atgtatacct acgagtaccc ccgaatgctt gtcacggtcg atgccgtaat tttcaaggtc
    12181 gatataaact ataagccaac ccaccttcta ctcattgaac gtggcaacga accctacaag
    12241 ggacatttcg ctttacctgg aggcttcccc gaaattaagg agctactagt tgatgccgcc
    12301 gctcgtgaac tatttgagga aaccgggatt gcaggagttg aactaaaaca gattggtgca
    12361 ttcgatgata ttgatagaga cccacgcgat agaaacattg ccattgcctt ttggggagtg
    12421 gtaagcgatg ataaaattag tccgactgct ggcgatgacg ctgcaaaagc ccaatggttt
    12481 ccgcttgata atcttccccc gctggcattc gaccattcaa aaatagttaa ggcagctctt
    12541 ggctgcctta agagaggttt tccagaacag taaactgcct aaactatcat tccggcacct
    12601 actgttacat tagtggcttc atcaataagt atcaaacttc ctgtaacacg gttctggcgg
    12661 tacgagtcgt aaaacaatgg tttggttgtg cgaatggtaa tgcaagcaat atcattcatc
    12721 cctaccgatt tgtcgttgta atccttttcc agggtgttga tgtttatctt gtacttcacc
    12781 tccttgacca tgcagcgcaa atcgttagtg gtgtgcttca gggcatactt accaccggga
    12841 accatgggtt tatcgcctaa ccaacatatc attagctcaa tgtcctgccc ctgcttaatg
    12901 ccattctcct caggaataat catacaccct ctggatacgt ccaaatcgtc ctccaaaata
    12961 attgttccgg attgcggagc ggtaattgaa tcaaggtgat cagtccaaaa atcgataccc
    13021 ttgatggtag tggtaaagcc cgagggtaaa actttaaccc tatcaccaac cctaaatgta
    13081 ccacttgcca tccgtccggc ataacccctg taatcgtgga actcaatgga ctgtggacga
    13141 ataacatact gaacagggaa tcgagcatcg agctgattat aatcatttga aatatacagg
    13201 ttctcaagca ggtacatcag tgtagggcct tgataccaat ccatattctt ggaacggtca
    13261 accacattat cgcctagaag agcgctaatg ggaatgaatc ggacatcgtg caaatcgaga
    13321 tgcgttgcaa attcatggaa cttctgctga atgttatcaa aaacatcctg cttaaaatca
    13381 actaaatcca ttttattcac gcatactatc acgtgtggaa tacggagaag cgaggcaatg
    13441 taagcatgac ggatggtttg ttcaatcacg ccatggcggg catcaaccag gataacggct
    13501 gcattggcag ttgaagcacc ggttaccata ttgcgggtat actgtacgtg acctggagta
    13561 tcggcaataa tgaacttgcg cttgggggta tgaaaataac ggtatgccac atcaatggtt
    13621 ataccctgct cgcgctcggc gcgcagacca tcggttagta aggcaagatt gatatactct
    13681 tcgcctttct ttatacttgc ccgttcaatt gcttccagct gatcctgaaa gattgccttt
    13741 gaatcgtata gtagtcgtcc aatcagagtg cttttgccat cgtcaacact gccagcagta
    13801 gtaaaccgca aaagctccat atctaggtat gaacgttcaa ttactttatc ttccattgga
    13861 aaatgattta aaaactaaaa ataaccttca attttgcgat cctccatggc tgcctcggag
    13921 cgtttatcgt cagcacgacc gccacgttca gttactcgcg ttgcagccac ctcacgaata
    13981 atatcctcaa cgctacttgc aaccgactca atagcacctg tgcaagtggc atcgccaatg
    14041 gtacggaaac gaacaatccg tttttctgct ttttcggtat ccttcatctt taagaaggga
    14101 gttttggcat aaagcacacc atcgcgatca aaaacctcac gttcgtgcga gaagtatagc
    14161 gagggtatag caatattttc cataagtata tattgccata catccatctc ggtccagttg
    14221 ctaattggga ataccctaaa atgctcaccc atattcttgc gaccattaaa aaggttccag
    14281 agttcagggc gttggttttt gggatcccac tggccaaact catcgcgatg cgagaagaaa
    14341 cgctcctttg cccttgcctt ttcctcatcg cgacgcccac ctcccatggc acaatctatt
    14401 tttaattcct caatggcatc gagcagggta acggtttgca acttgtttcg gctggcattg
    14461 taaccggttt cctccactac cctaccctta tcaattgagt cctgcacgta tcttacaatt
    14521 agctgcactc cggtttcctg caccaaccaa tcccgaaaat ctagtgtttc ctgaaagtta
    14581 tggccggtat cgatgtgcat tagggggaag ggaacctttg cgggataaaa agccttgcgg
    14641 gctagatgaa acatcactat ggaatccttt ccaccgctga aaagcatcac agggttttca
    14701 aactgtgaaa ccacctcgcg aataacaaac actccttccg attcaagttc gcgtaagtgg
    14761 ttaattggca ctttattact catatcaaaa tccttttaat tacttcaatt ttagcaactt
    14821 gagagattac atcaggtaac ccttaacatg cttgcctact tggtgttttt ccctaaacaa
    14881 tattcctata gcttttcatt tgcctctatc gctttcaaca ccctataagc atggcaaaga
    14941 tacaccatag ggtagtatga aatgattgtt atttatcaac ccatcgcagg aaatggtcgt
    15001 agagaatgtc agcagcttca ccctcactta attcggcagt atttaagcgc aagctgggat
    15061 tcataggtgc ctcgtagctg tcggatatcc cggtgaagtt tggaagcgaa ccgttaattg
    15121 ctttgctgta gtttcccttg atatcacgcg actgacaaac ctctactggg gcatcggtaa
    15181 atacctcaaa gaagtttttt gagccaacaa ttgaccttgc catttcgcgc attgatatca
    15241 gtggtgaaac gaaacaacag atggttacaa ctccctgtga attaagtaat gctgccatat
    15301 tggccaccct acgtatgttc tccgttctat cggctgggct gaatcccaaa tcgctacata
    15361 aaccctttcg aacctcatcg ccatcgagca gcaccacagg aattttaagc tgattaatac
    15421 ggccttgtag tgctttccct agtgttgttt ttcctgaagc gggtaaccct gttatccaga
    15481 ctgtgatagg atgcttataa ctcatgaaaa ctacctcata tcaataactt caaccccttt
    15541 gtgcttttcc acatcaaatt tgaaccgatc gtcacgtaaa ggaacgtttg atttgaagtt
    15601 tttaatttga atgatgtagt tattgccatc cttaccctgg taacgaataa ggtatggttc
    15661 aagggtgttt gcatcgaact gcaacttaac cgatgagtaa ggtaaattca ggtcgcgtgg
    15721 gaaaaaatca acctcatgca aaaccctgtt ctttgatttc tgctgcccga tatacttata
    15781 cttgaagttc ttgtcgtaat ccttaaaaag cttcattgga tcgtccaaaa agctattctc
    15841 cttaatattc atctttgaaa tggtaacctc ttttgcctcc ttaatgtatt gccatttggt
    15901 ttgaccatcg gaaaatactt ccatgcccat taggtcgagg taaaacattt tacccttagc
    15961 cagtatctta ccctgatggg tatcggtaat gcgttcctgg aggttttcaa gtgtaaagct
    16021 aaatgttgcg gaaagcgatt taatttcctg cattttgtta ctaaaactcc taacaattcc
    16081 atcggggttt acctcttgac taaagagagt aagcgacgat gaaataaaca gcaaactcag
    16141 aatttgtttc attgataaaa gaatttattc gtgcaaaaat acaaaattag aaactaatct
    16201 acggataaag tgttcaaaat acgttccaaa gacacctcgt caacaattaa aacctgtcgg
    16261 ggtttgccac cgtcggcagg gccaacaatt cctgcagctt ccagctgatc cataatgcgt
    16321 ccggcacggt tatacccaat ggaaaatttg cgttggataa gggatgttga acccgactgg
    16381 gtttgcacca caaggcgagc ggcatcctca aacagttcat cacgtttccg caggtctaca
    16441 tcaactgggc catcctgacc ctcaggaaca tactcgggta gctcataggc atgaggatat
    16501 ccgggctggt tacctataaa gtcggtaatc ctctcaattt cagggatatc aacaaaggca
    16561 cattgcaccc taaccaaatc gcttccggca gacactaaca tatcgccgcg gcctattagg
    16621 ttatttgcac ctggcgtatc gagaatggtt cgtgaatcga ccattgacga caccctgaaa
    16681 gcaatacgtg ccgggaagtt tgccttaata acacccgtaa tgatgttggt tgatggacgt
    16741 tgcgtagcaa tgattaggtg aataccaatg gcgcgtgctt tttgggcaag gcgcccaagg
    16801 ggcatctcaa tttcacgccc cgctgtcata attaaatcgg caaactcatc aataataacc
    16861 actatgtaag gcaaatacct atggccgttg ttaggattga gcctgcgagc cacaaacttc
    16921 tgattatact cctttattgt tcgaacctta gccaggttta gaagcttata ccgatcctcc
    16981 atctcaacgc ttagcgagtt cagagtgtaa ataacctttt gggtatcggt aataatggcc
    17041 tcaggagaat cgggtagctt ggccaggaaa tggcgttcga gcttgttgta aagggttaac
    17101 tctaccatct tggggtcaac aagaacgaac tttatttcag ctggatgctt cttgtaaagt
    17161 aacgacgtga taattgcgtt aagacctacc gattttccct gcccggttgc acctgcaaca
    17221 agcaggtggg gcagtttggt aagatccagc ataaaaatct cgttcgaaat ggttttaccc
    17281 aatgctattg ccaaatcgta tttacactcc tggaactttt gcgacttgat aattgagtgc
    17341 attgacacca cctcggggtt ttggttgggc acctcaatgc caattgttcc ctttccggga
    17401 attggggcaa ttattcgtat tcccagtgcc gaaaggctaa gcgcaatatc atcctctaaa
    17461 ctcttaatct tgctgatcct aacacctggg gcaggaacaa tctcgtaaag agtaacagtt
    17521 gggccaatgg tagccttaat cttatctatc tgaattttat agtgcgaaag ggtttctaca
    17581 atcttgttct tgttttcaac cagcatttca ttggttactg gcgattcagt attcttgtaa
    17641 tcctccagta gttcaattaa tggtttttga tagcttgaaa gttcaagcgt gggatcgtaa
    17701 agttcctgtt cgttaatatc ggatatggct agctctttct cctcgtttcg ctcnnnnnnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcaaaga actgaacagg aagaaggtgc
    17881 cactctgaaa gtgataaagc gatttaggca ttacgtatat aatataagca ataagtgcta
    17941 ttagaaccag aaaggccgca ccaaccttac ctatgactgt cgataaccat tccgagacaa
    18001 ataaaccatg cgccccgccg agtccacttc ctaggtatcc gcctaattcc ttgaataaaa
    18061 aacctaaagt aagcgagcca accagtattc ccagcaaaac cttaaaaata aattttgtaa
    18121 cacgtcccct aatgaactta agcagcttaa ggcccaaaac catgaaaatt aaagggaatg
    18181 ccaaggctgt tatgccaaac caatgattta taaactggtt tgaaagtgaa gcgccaagtt
    18241 ttccacccca gttgccaatc ttttgttcag ttgatgagaa tattgaaacc cactcaatgc
    18301 tttgatcaac cttccaggtg aacagaaatg ataagaacga aagaaaaata taaacgccca
    18361 aaaggattag gaaaagacct acggtaaacc taatcctttc atccttgaaa agcgaatcgt
    18421 tgctaacatc cttactgtcc gagtcttcaa catcggtacc cttatccttc ctagccataa
    18481 tgcataatca atataaaacg atgtaaaatt aacctgtatt tgtaaaaaat agaaacctta
    18541 ctgctgaagt aaatagatta aatccgtcat ggtttgaaaa tcaacctttt tgtaatcctt
    18601 tgggatttta aacgcatcat tattgatatt ttccttggct acattacgtg ccctgagcct
    18661 catttcaaga ttattgaacc tcaagttaaa accaagcata accccatcaa tgttttcgaa
    18721 aggagtattc aggtttggat tgtcaattgc aatttgtttg gtgtaaataa tctcaaactt
    18781 attatcaggg ttatcggcaa agtatcccat tacgctcttg catttatagc caatataatc
    18841 gcaatctcta accaaagtat caatattaac ctttggaatt cgagcataaa gcgcggggta
    18901 atgcccgttg gtgtatggtt cactgtggta aagtttctta ttaaaaacct ttagcaaagt
    18961 agtaaactcc ttttttgatg gttggctaat aattgaaatt gttatagccc ctgagagccc
    19021 atcgatggtg ttaatggtgt tgttattgca aaaacgaaca accagtgagc tgggcaatat
    19081 ttttttgtcg attctactga gagttaaagt atcgtattca atgtcgtaat caattcgtcc
    19141 ttcacttatt tcctttgggc tttttgatat acagctgctt aatgagaata aaatcacagc
    19201 cacgccaaca agttggtata cacttctgtt catcaacacc ttagttcaaa ttattaaatc
    19261 ataaaaatag aaaatgtttt tgtggtatgc aaaagtttga ggggcgtaat agttatggta
    19321 aaaccctgat cagccatcaa acatgttatt aaacatacat ataaaccatt gagtgatagt
    19381 tttacatcac gtgtattttt attaggattg caaaaaatag ttagttttgt aacttcaaag
    19441 taaaaaattt aatttactgt ttatgagcaa attagcagtt gtagcattag gcggaaacgc
    19501 cctcttgcgt ggaaatcagg tgggtaccat cgatgagcaa gaacagaata ccaccgatac
    19561 ccttgagaat ctagtttact taataaagga agggtataac cttgtaatca gccatgggaa
    19621 cggtccacag gttggaaata tcctcatgcg aaacgatgct ggcgagcagc tctatggcat
    19681 tcctcagatg ccacttgata tttgcgttgc cgattcgcag ggggtaattg gttacatgat
    19741 tgagcgcatg cttaggaatg tattgcgcaa acatggtatt gaccgtgaag tatgttgcct
    19801 ggtaacgccg gtaatcgttg atcacgacga ccctgccttt accaacccca caaagcgtgt
    19861 gggtcgtatt tacgataagg ctcaagccga cgctctggct gctgaaaagg gttggatatt
    19921 taaggaagag gttaaggata caggaagcgg ttggcgcagg gtagttcctt cgcctaagcc
    19981 caaagggatt ctaaacatca aggtaatcga tcaacttgtt cgtcagggta ttattgttat
    20041 tgcctcaggc ggaggcggtg taccggttta tattgacgag gagaagaatg ttagaccagc
    20101 cgaagcggta attgataagg atctggcatc ggcgcttatg gcagccgaaa ttaaagccga
    20161 tgaattttac atcctaaccg atgttccatt cgtgtatctt aactacaaaa agccgaacga
    20221 gcagaaactt gagttcctca actatgccga taccatgaaa taccttgaac agggaatgtt
    20281 tggtgagggt aacatggctc caaaaattaa agcatgtttg gactttataa agcgcggtgg
    20341 caaaatgagc gtgattaccg aagccaccaa gcttgaggat atgtcatatg gtaccaaaat
    20401 tacaatggag tacgaagatt aatcaataaa ccataaaaaa ctaaaagcta tgtcgttcaa
    20461 cttacgtaac cgcagctttt taaagctgct cgatttcaca ccaaaggaaa tccaattctt
    20521 gctcgacctt tcagctgatc taaaaaaggc aaagtatgcc ggaac
//
LOCUS       DAOOWJ010000041        20244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2648_length_20244_cov_7.940512, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000041 DAOOWJ010000000
VERSION     DAOOWJ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 20244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20244
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2648_length_20244_cov_7.940512"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1514)
                     /locus_tag="PLW22_05710"
     CDS             complement(54..1514)
                     /locus_tag="PLW22_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOU74234.1"
                     /translation="MSKVNIKLERLGEMFPENWTEEQRAKGKTLFLKYLSEKAHKHYG
                     GKIQTAPKAPVLGFNWFNVWYTPGVSKISTTIRDNNDASFELSNRGNLVAVVSDSTRV
                     LGDGDCTPPGGLGVMEGKAFLMKYLGGVDAVALCIDSKGKDGKNDPEKIIDFVKMCQH
                     SFGAVNLEDISQPNCYRVLDVLREECVIPVWHDDAQGTACVTLAGLLNALKLVNKKLK
                     DVRIVFFGAGASNTTIARLMIADGGDPKKMIMFDSHGALHRKRDDIKADSRFYRKWEI
                     CEQTNPDCINTMEEAMKGADVLISLSTPGPDTIKQDWIRLMADKAIVFACANPVPEIY
                     PYAAKAAGAYIVATGRGDFPNQVNNSLGFPGILKGALLVRASKITDNMAIAAAHSLAN
                     YAEKRGINPDNIVPTMDEADVFPVEAADVAVQAVKDGVARRPMSWDEAYSIAKKDIEY
                     SRSMVKSLHDNGFITDPPTEMLEEALEWTIKQIEGK"
     gene            1713..2585
                     /gene="sucD"
                     /locus_tag="PLW22_05715"
     CDS             1713..2585
                     /gene="sucD"
                     /locus_tag="PLW22_05715"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOU74235.1"
                     /translation="MSVLVNKSSRVIVQGFTGGEGTFHATQMIEYGTNVVGGVTPGKG
                     GQTHLDRPVFNTVSEAVKETGADVSVIFVPPTFAADGIMEAAAAGIKVIVCITEGIPT
                     NDMVKVKHFLADYPETRLVGPNCPGVITPEEAKVGIMPGFIHKKGHVGVVSRSGTLTY
                     EAVDQLTKLGLGQSTCIGIGGDPVIGTTTLEAVKLLMADPDTHGIVMIGEIGGSMETD
                     AARWIKEHGTKPVVGFIAGQTAPKGRRMGHAGAIIGGADDTAAAKMKIMRECGIHVVE
                     SPANIGQTMKDALK"
     gene            complement(2649..3065)
                     /locus_tag="PLW22_05720"
     CDS             complement(2649..3065)
                     /locus_tag="PLW22_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74236.1"
                     /translation="MNSIVSRPKSEKYYSENKIKILIDGQLRGKIGQGESLTVELPDG
                     EHTFRATTNFKMGSSEITVNTQKETNIEVSVNPNFNFTPTMAFTAIPFFLIVFFNYDN
                     LWVRITLGALMAATIFLFIRMLIKGKTEAINLKVKD"
     gene            complement(3143..3742)
                     /locus_tag="PLW22_05725"
     CDS             complement(3143..3742)
                     /locus_tag="PLW22_05725"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HOU74237.1"
                     /translation="MKLKVQHQERYSRLELLLRTFFGWLYILLPHGFLLFFVFIWALI
                     LQIVAFWAILFTGKYPKSLFDFQVGLFRWKLRVNARVLNISDGYPSFGINGTDEYTSL
                     EVEYPERVSRALMLVRFFFGWLYVYIPHLFILFFRQIFVGVIIILAWLIVLITGKYPR
                     EFHSWVVGQLRWMTRVGLYMGYMTDVYPAFTGDELDSEK"
     gene            3923..5314
                     /locus_tag="PLW22_05730"
     CDS             3923..5314
                     /locus_tag="PLW22_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU74238.1"
                     /translation="MRQKNLFSLRNILFAALIAATMVFASCKQQPKEAEPFRVEHANW
                     VYNATIYEVNVRQYTPEGTFKAFEQHLPRLKDLGVDILWFMPIHPIGVENRKGTLGSY
                     YSVKDYYGINPEFGTLDDFKSVVAKAHELGMKVIIDIVANHTSHDAVLLTEHPDWYVR
                     DSSGRVVSPFDWTDVAKLDYSKPELRRYMIDMLKYWIKDIDLDGFRCDVAAEVPTDFW
                     NEARAELNQLGKPIFMLAEAETPELQKYAFDADYAWEFHHIMNGIAQGKKSVVELEQY
                     LLKKAATYPKNTIKMNFITNHDENSWNGTEFERMGDAVKTMAALTFVIDGMPLIYTGQ
                     EVGLNHRLQFFEKDQVDWTDSKGLTDFYKKLTALKKSAKVLDAAEQGADIFRITTTAD
                     SSIFAFTRENETQKLFAVFNLSAKEQTFNFTGDAHLNTSYVEYFSNSSKEFTKDERVT
                     LKPWEFLIYLNNK"
     gene            5531..6718
                     /locus_tag="PLW22_05735"
     CDS             5531..6718
                     /locus_tag="PLW22_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOU74239.1"
                     /translation="MYYNIPVVGKVHWIGVNDRRKRLFENIWPLDRGVSYNSYLIVDE
                     KTALIDTIEDRAAGNYIERIKALLNGRTLDYLIINHMEPDHSGEIKDIFEQFKGVKLV
                     GNAKTFKMVEAYWGINENLIQVDDGDTLDLGFHKLKFVMTPWVHWPETMMTFDQTEQI
                     LFSGDAFGSFGALDGGIFDDEINFDFFEDEMRRYYSNIVGKYSNMVQKAFAKLKDVPV
                     KVIAPTHGPVWRSNPNKVIELYDRWSRYEAEEGVVIIFASMYGNTEAIADYIGRKVAE
                     NGIKNIRIHDISRTHISHLINEIWKYRGLVLGSCAYNSEMFPLMEQLTRELTHMRIKN
                     KKLALFGSYSWNGGGVKNLLKFAEEAKLELVAEPAEIYGKPNAEKLKQFDLLAQKIAE
                     SVK"
     gene            complement(6816..7085)
                     /locus_tag="PLW22_05740"
     CDS             complement(6816..7085)
                     /locus_tag="PLW22_05740"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU74240.1"
                     /translation="MSKVIIEKLTDEQINKMGIKNWPIWTKEISTFDWYYDTDEECLI
                     LDGEVDVKTDEGTFTVRTGDFVTFKKGLKCVWTVKKPIRKHYNFK"
     gene            7181..7570
                     /locus_tag="PLW22_05745"
     CDS             7181..7570
                     /locus_tag="PLW22_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU74241.1"
                     /translation="MEFNIPLGIEFTATEMVTKDNTASKYGSGLVDVFATPAMVALME
                     KAALNAVLPHLPHGFNTVGTEICVKHTKATPLGWEVHSKATLKEVDGKKLVFDVVAWD
                     SEGEIGRGTHTRYIIDSKRFMEKFSSK"
     gene            7580..8338
                     /locus_tag="PLW22_05750"
     CDS             7580..8338
                     /locus_tag="PLW22_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOU74242.1"
                     /translation="MNFDTYQFPKYHRHKLKVHSYEVDFNQRLTVTALFNYLQEIAWE
                     HAHLLGYGWEHLLQKGWFWALSRVEVEIYRLPAWTDEVVLLTWPRGVDGIFALRDFEV
                     YDHLGNKIIAATSSWLVLNIQTRRPVRAEWYSDFDYATRSSLGRNATKLVEQSVSPEF
                     SEKFDVRIGDIDINQHVNNVRYIDWAYNTFSIEHFKSYFPKRVVVNFNAEGKANDSII
                     ASRFQNSQDESIVEIANASNGKNLCRLMFEWGKI"
     gene            8411..9952
                     /locus_tag="PLW22_05755"
     CDS             8411..9952
                     /locus_tag="PLW22_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOU74243.1"
                     /translation="MYNQQDIEQMQRKGITPEMVEQQIQSFKKGFPYMKLVKPATVGN
                     GIIRLDGEELDSLIEMYRDFEGSRVKFVPASGAATRMFKHLYEFQSDYPVKGLECFND
                     KGFNTVFTFFERIKEFPFFDKLYNELWMQGYKMDELIDSKDYMPIVNTLLGSEGLNYG
                     NLPKALILFHKYGDIARTAMEEHLVEGAQYARDQHGNVNIHLTVSPEHIAGFEQLIER
                     VKSYYENHYEVTLNITYSVQKPSTDTIAVDMDNNPFRNDDGTLLFRPGGHGALIENLN
                     DLGHEMVFIKNIDNVAPDHLKPLTVRYKKALAGMLLSYRGIIYKYLQELDSQNLSVDE
                     LLAILDFTRDELCVIPPASLNTSDPAELNKYLFSVLNRPIRVCGMVKNEGEPGGGPFW
                     AVNPDGTTSLQIVESSQIDMNDPTSKAILNASTHFNPVDIVCSFIDFKGNKFNLLNYR
                     DPQTGFISVKSKDGKQLKALELPGLWNGAMSNWNTIFVEVPLATFSPVKTVNDLLREE
                     HQPCR"
     gene            complement(9958..10713)
                     /gene="truA"
                     /locus_tag="PLW22_05760"
     CDS             complement(9958..10713)
                     /gene="truA"
                     /locus_tag="PLW22_05760"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOU74244.1"
                     /translation="MAINRYFIYLSYRGTNYHGWQIQENATSVQNLVNNALSILLKEN
                     IQTIGAGRTDTGVHARFYVAHFDSVASNLESDGKFLHSLNRILPNDIAIHQIKKVNPS
                     ANARFDATLRGYEYVICRNKDPFWIGLSWFYDAQLNIDNMQLAAQQLMNYTDFTSFSK
                     VGSETKTNNCQIYKAEWNESGNLLIFTIEANRFLRNMVRAIVGTLIDVGRGKITPEEF
                     AQVIEKRDRKLAGTSAPAEGLYLTRIEYPDNLI"
     gene            11426..12427
                     /locus_tag="PLW22_05765"
     CDS             11426..12427
                     /locus_tag="PLW22_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU74245.1"
                     /translation="MSVQSTLDIKELNERIRLESSFVDIINIEMGKVIVGQKHLVESL
                     LVGLLADGHILLEGVPGLAKTLAINTLANIIDVKFNRIQFTPDLLPADLIGTLIYSPK
                     SEQFTVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGDETYKLEEPFLVLA
                     TQNPIEQEGTYPLPEAQVDRFMLKVKIGYPKLEEERLIMRENLAMQFPKPEKVLKPTD
                     IVKARQVVREVYMDEKIEKYILDVVFATRYPAQYNLQRFEPMISYGASPRASISLGLA
                     SKAYAFIKRRGYVIPEDVRAVCFDVLRHRIGLTYEAEAENITSEDIITEILNTIEVP"
     gene            12433..12852
                     /locus_tag="PLW22_05770"
     CDS             12433..12852
                     /locus_tag="PLW22_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74246.1"
                     /translation="MKPLAIIFLSVAIAFLSLSASAQNYIGLHKDEIAKRMPQENKGF
                     YFEKEVNVDDRGFLKYVNTLDEQTILFMIDTDGFCTAVSRMYNTWLYDQVVNELNSKY
                     QQVSTNKWLEIKEGKSYDLTLIKGKWFLTLTIRPHSK"
     gene            12861..13751
                     /locus_tag="PLW22_05775"
     CDS             12861..13751
                     /locus_tag="PLW22_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU74247.1"
                     /translation="METAELLKKVRRIEIKTRGLTRQIFAGQYHSAFKGRGMAFSEVR
                     EYRVGDDIRSIDWNVTARYNQPYVKVFEEERELTVMLLIDVSGSQNFGSTQHFKRHVI
                     AEVAAVLAFSAIQNNDKIGVILFSDKVEKFIPPQKGSKHILRIISELLNFEPSGKETN
                     IAEALRFLTNAIKKRSTAFVLSDFIDFNEQNYQPRFTDAISIANNKHDVVAIRIYDDR
                     ETQLPPVGLVRFTDAETGNDIWIDTANKLVQNYYSQWWNITTENLKQTFTKAQVDWVS
                     VRTDEDYVKPLMLLFKRRSK"
     gene            13748..14752
                     /locus_tag="PLW22_05780"
     CDS             13748..14752
                     /locus_tag="PLW22_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74248.1"
                     /translation="MKLIRFVFIVFLLLTLNVDAQIEQLEIKAVLKADTILIGDRVEF
                     AIIANGKGNFSVSLPVLADTLPGGIYIIGKPIIDSTIKDNTRSYSFKLPVTAFSPGNV
                     SVSKFPVLVKHGGSVDTTWITTDSLFVKLVPRDTTLKDIKDIKPPIKEPIRFSEVAPW
                     AGLGLLFAAIIVLLVMYLRSRKQNKPFLNIFKPKEPAHVVALRELMLLEENREWASDN
                     PKLFYTKLIDILRNYLEGRFHINAPEQTTSEILSEIARLDFDFFPYIETLRELLFTSD
                     LVKFAKHVTTIDENRRFLSFAINFVHSTKPIESSNDGENPTDEIKSADNTDLSTSYSN
                     "
     gene            14759..15748
                     /locus_tag="PLW22_05785"
     CDS             14759..15748
                     /locus_tag="PLW22_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU74249.1"
                     /translation="MLLVVYANPKFLYLLLLIPSLVGWYIYRLHKSRAALVISTAKHF
                     DNTGKSVKVYLRHLPFALRMLALALAIIALARPQSTNIQRNVTTEGIDIVLSLDVSGS
                     MLARDFKPDRLEAAKSLGINFISGRANDRIGLVIFAGESFTLCPLTTDRATLINQFKA
                     VDIGVLEDGTAIGSGLATAISRLKDSDAKSKVIILLTDGVNNRGEIAPMTAAEMAKTF
                     GIRVYTIGVGTYGTAPYPVQTPFGTRYQDVQVEIDEDLLKSIAQMTGGEYFRAINNKA
                     LEQVYQKIDQLEKSKIEVNEYAKREEKYRGWLLAAFILLLLEFVLRRTYLLGI"
     gene            15762..16805
                     /locus_tag="PLW22_05790"
     CDS             15762..16805
                     /locus_tag="PLW22_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU74250.1"
                     /translation="MFRFSNPEYLYLLLLIPVIIGLFWYSVRKQKQWITSFGIYKTVL
                     KLMPGHSFKRYWIKFIIATAAYIFIIIALAGPQVGAKLTEVKRKGMEMIIALDVSNSM
                     LAQDIKPNRIERAKQAISQLIDRFNNDRIGLIVFAGDAYIQLPVTNDYASAKLFLSSI
                     NPGMVPKQGTAIGRAIELAVNSFSPNANTGKVIVLISDGENHTDDPIEAAAMAAENGI
                     SIYTIGIGSPEGAPIPLGNNQEFLKDADGKVVITRLDEETLTKIAVTANGKYVRASNT
                     QFGLLPIYESLKKVDRRAIKDKVYSEYNEQYQYILAIAIALLFAELIILDRKTSLLSK
                     VNLFGADKTREDI"
     gene            16802..17530
                     /locus_tag="PLW22_05795"
     CDS             16802..17530
                     /locus_tag="PLW22_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74251.1"
                     /translation="MRKDIVLIILLAGFTVNTFAQSEHKLIRRGNRAYSKNDYLESEV
                     QYREALERNKHSFKANFNLGDALYKQNKFSDSDSIFNGINTSALSDADKSMIYYNKGN
                     SLFKQNKFKESADAYKMALKYNPNDIDAKYNLSEALRMMQNQQNQNNNQQQNNSNDSK
                     DNKDQKDDKKQDKKEDKKDNNNKQDNQQNNQNKNNQQQQQPKISKEDAERMLQALQQR
                     EKDIQDKLNKKQAKPVSGASIKNW"
     gene            17536..19362
                     /locus_tag="PLW22_05800"
     CDS             17536..19362
                     /locus_tag="PLW22_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOU74252.1"
                     /translation="MKRYLSGILLLFISGYLQSQEVTLQAYAPNLVEVGEQFSLSFTL
                     NAKPSEFKAPSIVDFDILAGPSTSSSTSIEVINGKVTQSQSYTYTYILVASKEGKYTI
                     DPAEAVVGKERIRSNPITIEVVKTTSGNAASRASQPASRNVRETQTDDLPGDELFVTI
                     ELTRKSAYVGEPIEAVIKIYTRVSIMAFEDAKFPAFDGFWSQEIKSSPNVNFERANVN
                     GKIYNAGVIRRYLLFPQKADKIEIDPFELVVVYQGHRSGPRSIFDEFFGGGFETYRKR
                     LVSKPVSINIKKLPLPEPQGFTGAVGSFKIDASVDKNTLKANDAFTYRLKISGKGNLK
                     LIGNPKLNFPSTFEVFDPKISENISVDEGNAMGSKTYEYVCIPRAGGNFSIEPAVFSY
                     FDPDKESYITLKTSAISVAVSADSSSTGNVMVASFGKEDIKFIGKDIRFIKTSSSAFK
                     SKSAFWVTSGYYQLLYILLIALFITFSYLFRRYRQRMQDVALVRTRRASNVAQKRLKM
                     ASQLLEQNNNAQFFEEIHKAIWGYVSDKLNIPLASLSLDNASEKLTQQGVDADRISTF
                     RSIVETCEYARFAPMAEHSQMTDIYEKTFSLIEDLEDTLKRK"
     gene            19388..20152
                     /locus_tag="PLW22_05805"
     CDS             19388..20152
                     /locus_tag="PLW22_05805"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74253.1"
                     /translation="MKRILYLALMIVPVSLFGKVTMQNPDSLFALANKYYTEGKYSEA
                     VTHYEAIVANGKVSAELYFNLGNAYFKLNDIAHAILNFERAYLLNPGDDDISFNLELA
                     RAYTTDKIEAIPDFFVVKWVKSISNLFSSNTWAFLAISSFIIALILFMIFQFSGKYLI
                     KKYAFIISWFSAFIFIVSLAFSVSQKNRIVNSNHAIVTSSAVSVKSSPSESSNDLFIL
                     HAGTKVETLRNVGEWCEIRIADGNKGWLPKSTFERI"
BASE COUNT         5936 a         4107 c         4252 g         5949 t
ORIGIN      
        1 accctttatc ctttatcctt tatcctttat cctttatcct ttatccttta tccttacttc
       61 ccctcaatct gtttaatagt ccattcaagg gcttcctcca gcatttcggt tggtgggtcg
      121 gttatgaagc cattatcgtg taacgacttt accatggaac gagagtattc aatatccttt
      181 ttggcaatgc tgtacgcctc atcccagctc attggcctac gggcaacgcc atctttaaca
      241 gcctgtacag ccacatcggc tgcttcaacg gggaatacgt cggcttcatc catggttggg
      301 acaatattat cgggattaat tccacgtttc tcggcgtagt ttgccagtga gtgagcagcg
      361 gcaatggcca tgttatcggt tatttttgaa gctctaacca gcaaggctcc tttaagtatt
      421 ccagggaatc ctaacgagtt atttacctgg tttgggaaat cgccacggcc cgtagccaca
      481 atgtatgccc ctgctgcttt tgctgcatat gggtaaattt caggtacggg gttggcgcag
      541 gcaaaaacaa tggctttatc ggccataagc ctaatccaat cctgcttaat ggtgtcaggg
      601 cctggggtgg aaagcgatat tagcacatcg gcgcctttca tggcctcctc catggtgtta
      661 atgcaatcgg gattggtttg ctcgcaaatc tcccatttgc ggtaaaaacg gctatcggct
      721 ttaatgtcgt cacgtttgcg atgaagtgca ccatgtgagt cgaacataat cattttttta
      781 ggatcaccac catcagctat catcaggcgg gcaatggtgg tattggaggc accagctcca
      841 aaaaatacaa tgcgaacatc ttttagcttt ttatttacca gcttcaatgc attgagtaac
      901 ccagcaaggg taacgcatgc agttccctgg gcatcatcgt gccaaaccgg aatcacacat
      961 tcctccctca gtacatcgag aactctatag cagttaggtt gtgagatatc ctcaaggttt
     1021 acagcgccaa agctgtgctg gcacatcttt acaaaatcga ttatcttttc tgggtcgttt
     1081 ttgccatctt tccccttgct gtcaatacaa agagcaactg catccacgcc accaaggtac
     1141 ttcatcagga atgctttccc ttccatcact cccagaccac ccggaggggt gcaatcgcca
     1201 tcgccaagta cacgggtcga gtcgctaaca acggcaacta aattgcccct gttcgaaagt
     1261 tcaaatgacg catcattatt atcgcgtata gttgttgata ttttggagac tcctggggtg
     1321 taccaaacat tgaaccagtt aaagccaagc acaggtgctt tgggtgctgt ttggatttta
     1381 cctccgtaat gcttatgggc cttctcgctg aggtatttta agaataaagt tttacccttt
     1441 gcgcgttgct cctcagtcca attttctggg aacatttcgc ctaggcgttc taatttgatg
     1501 tttactttac tcatatgttt aatttttaat tgattagatt tcgatttgct gatataattt
     1561 ttacccaaga tacaaaattc aaactaaaca atctagtttt ttcaatatgt tatacagtat
     1621 gttttagttg ctttttttga caaaataatg taggtttgag gtcgcaatac gtgttgtatt
     1681 tttacgatta acgattcaac aaaatccatg atatgagtgt attagtcaat aaaagttcac
     1741 gtgtaattgt tcagggcttt acaggaggtg aaggcacttt tcatgctacc cagatgattg
     1801 agtatggaac caacgttgtg ggtggagtaa ctccgggcaa gggaggacaa acacatctcg
     1861 ataggcctgt gttcaatact gtttccgaag cagtgaaaga aaccggtgcc gatgtatcgg
     1921 taatatttgt acccccgact tttgctgccg atggcattat ggaagctgcc gctgcaggaa
     1981 taaaggtaat cgtttgtatt actgaaggta ttcccacaaa cgatatggtt aaggttaagc
     2041 acttccttgc tgattacccc gagacccgct tagttggacc taactgtcct ggagtaataa
     2101 ctcccgaaga ggctaaggtt gggataatgc cagggtttat ccataaaaag ggacatgttg
     2161 gcgttgtatc gcgttctggc acattaacct acgaggccgt tgatcagctt accaagttgg
     2221 ggttgggtca aagcacttgc attggcattg gtggcgatcc ggtaattgga acaaccacgc
     2281 tcgaagctgt taagctacta atggccgatc ccgatacaca tggtattgtg atgataggtg
     2341 aaataggcgg tagcatggaa accgatgctg ctcgatggat taaggagcat ggaactaagc
     2401 ctgttgtggg ctttattgct ggacaaactg ctcccaaagg tcgccgtatg ggtcatgctg
     2461 gggctattat tggtggtgcc gacgataccg ctgctgctaa aatgaagatc atgcgtgagt
     2521 gtggcattca tgtagtggaa tcgcctgcta acattggtca aaccatgaag gatgcgctga
     2581 aataatattc taccaaaaac aataaaggcc ggcaaatatt gagccggcct tttttattcc
     2641 cgtattggtt aatcctttac ttttaagttt attgcctcag ttttcccttt tattagcatc
     2701 cgtatgaaca agaatattgt agcagccatt agagccccta atgtaattct tacccataaa
     2761 ttatcataat tgaagaaaac tattagaaaa aacggaattg cagtaaaagc cattgttggg
     2821 gtgaagttga agtttgggtt tacactaacc tcaatgttgg tttctttctg ggtattaaca
     2881 gtaatttcgc tacttcccat tttaaaatta gttgtggccc tgaaagtgtg ttccccatca
     2941 ggaagttcaa cggtaagcga ttcgccttgt ccaattttgc cccttagctg accatcaatt
     3001 agtattttga ttttgttttc ggaataatac ttttcactct ttgggcgcga tactattgaa
     3061 ttcatattca taactatttt aaaaaaaaag gcatcgtatt aaaccgatgt cttggcaaaa
     3121 atacaaaaaa gatgtttgta agctacttct ctgaatctaa ttcgtcgccg gtaaaggcgg
     3181 ggtaaacatc tgtcatgtaa cccatgtata atccaacacg tgtcatccat ctaagttgtc
     3241 ccacaaccca actgtgaaac tctctggggt atttacctgt aattagtact atcaaccatg
     3301 ccaaaatgat aataacgcct acaaatattt gcctaaagaa caaaataaag aggtgtggaa
     3361 tgtaaacata aagccaacca aaaaagaaac gaaccagcat taatgcacgg cttacccttt
     3421 cagggtattc aacctctaat gaggtatact catcggttcc attaatgcca aacgaagggt
     3481 atccatcgga tatgtttaat accctggcgt tcacacgtaa tttccatcgg aatagtccta
     3541 cctgaaagtc aaataaactt tttgggtatt taccagtaaa tagtattgcc caaaatgcta
     3601 caatctgcag gattagagcc caaataaata caaaaaatag gaggaaaccg tgtggcagta
     3661 gaatgtacag ccagccgaaa aatgttctca ggagcaattc taacctagag tatcgttcct
     3721 gatgttgaac ttttagtttc attttattta agttttaggt tagggagatt ttgaactcaa
     3781 acaagttaca gattttttta tttcaaatag tagaaatcat ttaaaattat tcctaaatta
     3841 gcaaaggcct gtaattacgg gcatttccta ttcgcaaacg tgtgaaagct gataaatcaa
     3901 tttcaaataa accaatagca atatgagaca aaagaatcta tttagcttga gaaacatttt
     3961 gtttgcagcc ctaattgctg caacaatggt atttgcctcg tgtaagcagc aacctaagga
     4021 ggctgagcca ttccgcgtag agcatgccaa ttgggtttac aatgctacca tttacgaggt
     4081 aaatgttagg caatacacac cggaaggaac attcaaagcc tttgagcaac acctgcctcg
     4141 cctaaaagat ttaggggttg atatactttg gttcatgcca attcatccca ttggagttga
     4201 gaaccgtaaa ggaacgctag gaagctacta ctccgtaaag gattactacg gtattaaccc
     4261 cgagttcgga acactcgatg actttaagtc agttgttgcc aaggctcatg agttgggtat
     4321 gaaggtgata attgacattg tggcaaacca cacctcgcac gatgctgttc tcttaactga
     4381 acatcccgat tggtacgttc gtgattcctc gggtagggtt gtttcaccat tcgattggac
     4441 cgacgtggct aagttggact actcaaaacc tgaactacgc cgttacatga ttgacatgct
     4501 taagtattgg attaaggata ttgacctcga tgggtttagg tgcgatgtgg ccgctgaggt
     4561 tccaaccgat ttctggaacg aggctcgtgc tgagctcaac cagcttggca aacccatttt
     4621 catgcttgcc gaggccgaaa cccctgaact acagaaatat gcgtttgatg ctgattatgc
     4681 ctgggagttc catcatatta tgaatggtat tgctcagggc aagaaatctg ttgttgagct
     4741 ggagcaatac ctgctcaaaa aagcagccac ttatcctaag aataccatta aaatgaattt
     4801 tatcaccaac cacgatgaga attcatggaa tggaactgag tttgagcgta tgggcgatgc
     4861 ggttaaaacc atggctgcac tcacatttgt tattgatggg atgcctctta tttataccgg
     4921 ccaggaggtt ggtctaaacc ataggctaca gttttttgaa aaggaccagg ttgactggac
     4981 tgatagtaaa ggtttgactg atttttacaa aaaactcact gcgttgaaaa agtccgcaaa
     5041 ggttcttgat gccgcagagc agggagccga cattttccga ataacaacca ctgccgatag
     5101 ctcaattttc gcatttacac gcgaaaatga aactcaaaag ttatttgctg ttttcaacct
     5161 atcggccaag gaacagacct ttaactttac cggcgatgcg catttgaata ccagctatgt
     5221 tgagtatttc agcaatagct ctaaagagtt cacaaaggat gagagagtaa ctctaaaacc
     5281 gtgggagttc ttaatctact taaacaataa gtaaagtttt caagagtaaa aaagaggctg
     5341 tcctcatagg gcagcctctt ttgattttat agaatttaaa aggttttacc tattacttga
     5401 tgatgctaac ttattcttaa gatactattt ttatttagat tgatttttaa caactggtta
     5461 gttgtttaac aaatcacttt aactttctac ttttgccata agattaaaaa caaaaaaata
     5521 cttaagcatt atgtattaca atatccctgt tgttggaaag gttcactgga taggggtgaa
     5581 cgatcgacgt aagaggcttt ttgagaacat ctggcctttg gataggggcg tttcctacaa
     5641 ttcatacctg attgttgatg aaaaaaccgc tctgatcgat actatagagg atcgggcggc
     5701 cggtaattac attgagcgta taaaggcttt acttaatgga cgtacgctcg actacctgat
     5761 tattaaccat atggagcccg accattccgg ggagattaaa gacatttttg agcaatttaa
     5821 aggggttaag ctggttggaa atgccaaaac ttttaagatg gttgaagctt actggggtat
     5881 taatgaaaac ctaatacagg tagatgatgg cgataccctt gacctcggtt tccacaagct
     5941 gaaatttgtg atgacaccat gggtgcattg gcccgaaacc atgatgacct tcgatcaaac
     6001 cgagcaaatc cttttctccg gcgatgcctt tggcagtttt ggcgcgttag atggcggtat
     6061 ttttgatgat gaaatcaact ttgatttctt tgaggatgag atgcgccgct attactcaaa
     6121 tatagtaggg aaatacagca atatggtgca aaaagctttt gcaaagctta aggatgtacc
     6181 tgtaaaggtg attgcaccta ctcatggccc cgtttggcgg tcaaatccca ataaagtaat
     6241 tgaactgtat gaccggtgga gccgttacga ggctgaggaa ggcgttgtta tcatttttgc
     6301 atcaatgtac ggtaatacgg aagctattgc cgattacata ggccgtaaag tggcagagaa
     6361 tggtattaag aacattcgca tacacgatat ttctcgcacc cacattagcc atttgattaa
     6421 cgaaatttgg aaatatcgtg gacttgtttt ggggtcatgt gcatataact cggaaatgtt
     6481 tcctcttatg gagcagctta cccgtgagct aacacatatg agaataaaaa ataaaaagtt
     6541 ggctcttttc ggcagctaca gctggaacgg cggtggagtg aaaaacctgc ttaagtttgc
     6601 tgaggaagcc aaacttgaac tggttgctga acctgccgag atatatggca aacctaatgc
     6661 cgaaaagctt aagcagttcg atttgttagc tcagaagatt gcagagagcg ttaagtagga
     6721 aaatacctga tgaaaaaatg aaaaagagac gtttaaacgt ctctttttta tttaaaaacc
     6781 attgcaagta ataagctttc ctgaactgga aggcattact taaagttgta atgtttccga
     6841 ataggctttt taaccgtcca aacacatttt aaccctttct taaaggtaac aaagtcgcct
     6901 gtcctaactg taaaggtacc ctcgtcggtt tttacatcta cctcaccatc aagaatcagg
     6961 cattcctcgt cggtatcgta ataccaatca aaagtagata tttccttagt ccaaataggc
     7021 caattcttta tgcccatttt gtttatttgt tcatccgtta gcttttcaat tatcaccttg
     7081 ctcatggttg cttaacttgg tttacatgca aaaatagaaa taaatgggct aagttgaaaa
     7141 ttgttcatat ctttgtaaaa gcactaaaaa cactaatggt atggagttta acattcccct
     7201 tggcattgaa tttactgcaa ccgaaatggt tacaaaggat aacactgcat caaaatacgg
     7261 ctcaggcctt gttgatgttt ttgccacacc cgctatggtg gcgctcatgg aaaaggcagc
     7321 tcttaatgct gttttaccac atctgcccca tgggttcaat accgttggta ctgagatttg
     7381 tgttaaacac accaaggcta cacctttagg ttgggaagtt cacagcaagg ccacccttaa
     7441 agaggtagat ggcaaaaagc ttgtttttga tgtggtggca tgggatagtg aaggcgaaat
     7501 tggcaggggt acccataccc gctacattat cgacagtaaa cgttttatgg aaaagttctc
     7561 atccaaatag tctagtttga tgaattttga tacctatcag tttccaaaat accacaggca
     7621 taagctaaag gttcattcgt acgaggtgga ttttaaccaa aggttaacag ttacggccct
     7681 gtttaattac ttgcaggaga ttgcctggga gcatgctcat ttactgggtt atggctggga
     7741 acatctgttg cagaagggtt ggttttgggc cctgtcgcgt gttgaagttg aaatataccg
     7801 gttacctgca tggaccgatg aggtagttct gctaacctgg cctaggggag ttgatggaat
     7861 ttttgcctta cgcgattttg aggtatatga ccatttagga aataaaataa ttgctgcaac
     7921 gtcgagctgg ttggttctta acattcaaac ccgtcggcca gtaagggctg agtggtactc
     7981 cgatttcgat tatgcaacta gaagttcatt agggcgcaat gcaaccaaac tggttgaaca
     8041 aagcgttagc cctgaatttt cggaaaaatt cgatgttcgc attggcgata tagatattaa
     8101 ccaacacgtt aataatgtta ggtatatcga ttgggcctac aataccttta gcattgagca
     8161 ttttaaaagc tattttccca aaagggtagt ggttaacttt aatgctgaag gtaaagcaaa
     8221 cgatagcata attgcatcac gttttcaaaa ttcgcaagat gagagcattg ttgaaattgc
     8281 gaacgccagc aacggaaaaa acttgtgccg gttgatgttt gaatggggaa aaatttaata
     8341 aatttaaaat taattttctt tcccaatcat tatgaaagtt ttaataaaat agaacccaaa
     8401 aaataacact atgtataacc agcaggatat tgagcagatg cagcgaaagg gaataactcc
     8461 cgaaatggtt gagcagcaaa ttcaatcatt taaaaagggc ttcccttaca tgaagttggt
     8521 taaacccgct acagttggca acgggataat tcggttggat ggagaggaac ttgatagcct
     8581 cattgaaatg taccgcgatt ttgagggtag tagggttaag tttgttcctg catcgggtgc
     8641 ggcaacccgt atgtttaaac atctctatga gtttcagtcc gattacccgg taaaagggtt
     8701 ggagtgtttt aacgataaag gttttaatac cgttttcacc tttttcgaga gaataaaaga
     8761 gttcccattt ttcgacaagc tttacaatga actctggatg cagggttata agatggatga
     8821 acttattgat agtaaggatt atatgcccat agtaaatact ctgctaggtt ccgaggggct
     8881 aaattacgga aatctgccaa aagcgcttat cctattccac aagtatggcg atattgctcg
     8941 tactgccatg gaagagcatc tggttgaggg ggcacagtat gctcgcgacc agcatggtaa
     9001 tgtaaatatt caccttacgg tgtcgcccga acacattgct ggctttgagc agctgattga
     9061 acgcgtaaag tcatactacg aaaaccacta tgaagtaacc cttaacatta cctactcggt
     9121 tcaaaagcca tcaaccgaca ccattgcggt tgatatggac aataatcctt tccgtaacga
     9181 tgatggcact ctactttttc gccccggcgg gcatggtgca ttaattgaga atctcaacga
     9241 tttaggccac gaaatggtgt tcattaagaa tatcgacaac gttgctccag accatcttaa
     9301 accgctaacc gttcgttata aaaaagcact tgcgggaatg ctgcttagct accgaggtat
     9361 tatttataaa tacctgcaag agctcgacag ccaaaacctt tcggttgacg aacttttggc
     9421 aattcttgac tttacccgcg atgagctctg tgttatacca ccggcatcgt taaatacctc
     9481 agaccctgcg gagctgaaca agtatctgtt tagcgtgctt aataggccaa ttagggtttg
     9541 cggtatggtt aaaaatgagg gagagcccgg tggtggccca ttctgggcag ttaatcccga
     9601 cggcacaaca tcgcttcaga ttgttgaaag ttcacagata gatatgaacg accctacttc
     9661 taaagctatc ctgaatgcat caacccattt caaccctgtt gatatagtat gttcattcat
     9721 tgattttaaa gggaataagt ttaacctgct taactaccgc gatccgcaaa ccggctttat
     9781 ttcagtaaag agcaaggatg gtaaacagct caaggcgtta gagcttccgg ggctttggaa
     9841 tggggccatg agcaactgga ataccatttt tgttgaagta cccttggcaa cattctcgcc
     9901 agttaaaact gtaaacgacc ttttgcggga ggaacatcaa ccctgcaggt aagcaagcta
     9961 tattagatta tcaggatact ctatccttgt aaggtataac ccctcggccg gagcagaggt
    10021 tccggccaat tttctatcac gcttctcaat tacttgggca aactcttctg gcgttatttt
    10081 tcctcttccc acatcaatta gagtgcctac aatggcacgt accatgttgc gtaaaaaccg
    10141 gttagcctca atggtaaata tgagcaggtt accactctcg ttccattccg ctttatatat
    10201 ctggcagtta ttggtttttg tttcggagcc aaccttgcta aagctggtaa aatcggtata
    10261 gttcattaac tgttgtgctg ccagctgcat attatcaatg tttagctggg catcgtaaaa
    10321 ccacgatagg ccaatccaga agggatcttt gttacggcaa atcacatact cataacccct
    10381 taaggtggca tcgaaacggg cattcgcgct cgggtttacc tttttaattt gatgaatggc
    10441 aatatcgttg ggtaaaattc tgtttaagct gtgcaaaaac ttaccgtctg attccaaatt
    10501 gctggctaca gaatcgaaat gcgccacata gaacctggca tgaacgcccg tatcggttct
    10561 acctgcacct atggtttgaa tgttttcctt tagcagtata ctcaaagcat tgtttactaa
    10621 gttctggaca ctggtggcgt tttcctgaat ctgccagcca tgatagtttg tgcccctgta
    10681 cgataggtaa atgaaatatc tatttattgc cattatactt gcttttgatc ttcctttatg
    10741 acatcgtttg ctcactatgt atttaaaaga aacatgaaaa aaaaaaggtc ccctgattcc
    10801 tgttcaataa tccattttgc attaaattac acaacaaata tactcgtata taaagctatt
    10861 ttgaaatatt tccgagattc gtccatagaa tttttctatt gacgaatttc ctctaaaaat
    10921 gtttctgtat ccacattttt attagcccgg ttgaacttgt taattcatgg tcgtttggtt
    10981 tagctgttca ctttttacta ttcctctaac ttcctctaac tccatcatat tctttctatt
    11041 ccgattcatt ccgtctcatt cccgcctcaa atcttgcgtt tcacggctca cggttcacgt
    11101 ttcacggact caaacttcct ctaacttctt ctaacttcct ctagcttcct ctaacttcct
    11161 ctaacttcct ctaactccat ctaactccat catattctct ctattccgac tcattccgtt
    11221 cattccgaat cattcctcaa aaatttaatt catggtactt ggatttattt aaaaatcaat
    11281 tataaaagtg tctgaaagcc tttaactacc tttattatag ctaaaatttc atttaacgaa
    11341 aattaacatc gcaggcatct atttattatc atatttgcaa ttgaggtttc atataaggtt
    11401 aaacaaattt taaaaagata tttttatgag tgttcaatcg acccttgaca ttaaggagct
    11461 aaatgagagg ataagacttg aaagttcctt tgttgacatt atcaacattg aaatgggcaa
    11521 ggtaattgtt ggccaaaaac accttgttga gtcgttactg gttggattac ttgccgatgg
    11581 acacattcta cttgaaggtg ttcctggctt agccaaaacg cttgccataa atactttggc
    11641 aaacatcatc gatgttaagt ttaaccgtat tcagttcacc cccgatttgc ttcctgccga
    11701 ccttattgga acacttattt acagcccaaa atccgaacaa tttaccgtta aaaaagggcc
    11761 aatttttgca aactttattc ttgccgatga aattaaccgt gctccagcta aggttcagag
    11821 cgctttgctt gaggccatgc aggaacgtca ggtaaccatt ggcgacgaaa cctacaagct
    11881 cgaagaacct ttcctggttt tggcaactca gaatccaatt gaacaggaag gaacctatcc
    11941 gcttcctgag gctcaggtcg accgtttcat gcttaaggtc aaaattggtt atccaaagct
    12001 ggaggaggaa cgccttatca tgcgtgaaaa tctggccatg cagttcccaa aaccagaaaa
    12061 agtcctaaaa cccaccgata ttgtgaaggc gcgtcaggtt gttcgtgagg tttacatgga
    12121 tgagaaaatt gaaaagtata ttctggatgt agtgttcgca actcgctatc cagcacagta
    12181 taacctgcag cgatttgaac ctatgatttc ctatggagcc tcacctcgtg catccattag
    12241 ccttgggttg gcttcaaagg cttacgcctt tattaaacgt cgtggatacg ttattcctga
    12301 ggatgttcgc gctgtatgtt ttgatgtttt gcgtcatcgt ataggattaa cctacgaggc
    12361 cgaggccgaa aacattacat cggaggatat aattactgaa atacttaaca cgatagaggt
    12421 tccatagcaa ccatgaaacc attggctatc atattccttt cagtagcaat cgctttcttg
    12481 agcctatcag ctagcgctca gaattacatt ggcttgcaca aggatgaaat agccaaacgg
    12541 atgccgcagg agaataaagg tttctacttt gaaaaggagg tgaatgttga cgaccggggc
    12601 tttttgaagt acgttaacac cctcgatgag cagaccatac ttttcatgat tgatactgat
    12661 ggcttttgca ctgcggtatc tagaatgtat aacacgtggc tttacgacca ggttgtgaat
    12721 gaactgaata gcaaatatca acaggtatca accaataagt ggcttgaaat aaaggagggc
    12781 aagagttacg atttaaccct gataaaaggg aaatggtttc ttacccttac catcaggcct
    12841 cactctaaat aggtatagcc gtggaaacag cagaacttct gaaaaaagtt cgacgtattg
    12901 aaattaaaac aaggggatta acccgccaaa tatttgccgg tcaataccat agcgctttta
    12961 aggggcgcgg tatggctttt agcgaggttc gcgagtatag ggtaggtgac gatattcgca
    13021 gtatcgactg gaacgttact gctcgttaca accaacccta cgttaaggtt tttgaggagg
    13081 agcgtgaact tactgtaatg cttcttatcg atgttagcgg ttcgcagaac tttggctcca
    13141 cccaacactt taaaaggcat gtaatagctg aggttgctgc tgttcttgcc ttttcggcca
    13201 ttcaaaataa cgataaaatt ggggttattc tcttttccga taaggtggaa aaatttattc
    13261 caccccagaa gggtagtaaa cacatactca gaataataag cgaattgctg aattttgagc
    13321 catcgggcaa ggaaacaaat attgctgagg cgcttaggtt cttgacaaac gccataaaaa
    13381 agcgcagcac tgcttttgta ctgagcgatt tcattgattt taatgaacaa aattaccaac
    13441 caaggtttac cgatgccatt agcattgcta acaataagca cgatgttgtg gctatacgga
    13501 tttacgatga tcgcgaaact caacttccac ccgttggcct tgtccgtttt accgatgccg
    13561 aaacaggtaa tgacatttgg attgacacag caaacaaact ggtgcaaaac tattacagcc
    13621 aatggtggaa tatcactact gaaaatctga aacagacttt taccaaagct caggtggatt
    13681 gggtatcggt gcgaaccgac gaggattacg taaaacccct aatgttgctt ttcaaaagga
    13741 ggtcgaaatg aagttaataa ggttcgtttt tatagttttt ttgctactaa cccttaacgt
    13801 cgatgctcag attgagcaac ttgaaataaa ggctgtacta aaagccgata caatactgat
    13861 aggtgatagg gtggagtttg caattatagc taatgggaaa ggaaattttt cggtatcctt
    13921 accagtacta gccgatactt tacccggggg gatatacatt attggtaaac ctattatcga
    13981 ttctactatt aaagataata cccgtagcta cagctttaaa cttcctgtta cggcattttc
    14041 tcccggaaat gttagtgttt ctaagtttcc ggttttggtc aagcatggtg gtagtgttga
    14101 tactacgtgg ataaccaccg atagcttatt tgtaaagctt gttccgcgcg acaccaccct
    14161 taaggatatc aaggatatta agcccccaat aaaggaacca atccgatttt cggaggtggc
    14221 tccttgggcc ggattaggct tactctttgc tgcaattatt gttctgcttg ttatgtactt
    14281 gcgtagtcgc aagcaaaata agccattcct caatatcttt aagccaaaag aacccgctca
    14341 tgttgttgct cttagggagt tgatgctgct tgaggagaat agggagtggg cttcggataa
    14401 tcccaaactg ttctatacca agctgatcga tattcttcgg aattacctgg aaggacgttt
    14461 tcacattaat gctcctgagc aaactacaag cgagattctt tctgaaatag ctaggcttga
    14521 cttcgacttt tttccctaca ttgaaaccct tcgcgagttg ctctttactt ccgacctggt
    14581 aaaatttgct aagcatgtaa ctaccatcga tgagaaccgt cgctttttga gctttgcaat
    14641 caattttgta cacagcacta aacctattga aagttcaaac gatggtgaaa acccaactga
    14701 tgagattaag agtgccgata ataccgattt atcaaccagc tattcaaact aaccggttat
    14761 gcttttagta gtttatgcaa acccaaagtt tttatacctg ttactgctaa ttccatcttt
    14821 agtggggtgg tatatttaca ggttacataa atcgagggct gcgcttgtaa tttcaacagc
    14881 taaacacttt gataatacgg gcaagtcagt aaaggtatat ttgcggcatt tgccctttgc
    14941 attaagaatg ttagcattgg cattggccat tattgcattg gccaggccac aatcaactaa
    15001 tatccaacgg aatgttacca ccgagggtat tgatattgtg ttatcgctcg atgtatcggg
    15061 tagtatgctc gcccgtgatt ttaagcccga taggcttgag gctgcaaaga gtttgggtat
    15121 taattttata agtggccgag ctaacgaccg cattggactc gtaatttttg caggtgaaag
    15181 ttttaccctt tgcccattaa ctaccgacag agcaactctg attaatcagt ttaaagctgt
    15241 agatattggt gtgcttgagg atggcacagc tataggctcc ggattagcca cagctatttc
    15301 ccgtttaaag gatagcgatg ctaaaagtaa ggtgataatc ctcctcaccg atggggttaa
    15361 taaccgtggt gagatagcgc caatgaccgc agccgaaatg gcaaaaacct ttggtattag
    15421 ggtttacacc atcggggtag gaacatatgg aacggctcct tatcccgttc aaaccccctt
    15481 tggaacccgt taccaggacg tgcaggttga aattgatgaa gatttgctga aaagtattgc
    15541 ccagatgact ggaggtgaat acttcagagc aattaacaat aaggcacttg aacaggttta
    15601 ccagaaaatt gaccagcttg aaaaatcaaa aatagaggtc aatgagtatg ctaagcgtga
    15661 agaaaagtat cgtggctggt tattggcagc ctttattttg cttttactgg aatttgttct
    15721 acgtagaacc tatttattag gaatttaatt ttaggattgc catgtttcgt ttttctaacc
    15781 cagaatatct ttatctctta ctgcttatac cagtaattat tggattattc tggtattccg
    15841 ttcgtaagca aaagcagtgg attactagtt ttggcatata caaaactgtc ctaaaattaa
    15901 tgccaggaca ttcgttcaag cgctactgga ttaagtttat cattgccact gctgcataca
    15961 tttttatcat tattgcactt gctggcccac aggtaggtgc caaacttact gaggtaaaac
    16021 gtaagggtat ggagatgatt attgcccttg atgtttccaa cagcatgcta gcacaggata
    16081 tcaaaccaaa tcgtattgag cgcgccaagc aagccatttc gcagcttatc gataggttta
    16141 acaacgaccg tattggattg atagtttttg ctggcgatgc ctacattcag ctccccgtta
    16201 ctaacgacta tgcatcagct aaactctttt tgtcatcaat aaatccagga atggttccta
    16261 agcagggaac tgccataggc agggctatcg agctggctgt aaattccttt tcgcccaatg
    16321 caaataccgg aaaggtgata gttttaattt ccgatggcga aaaccatacc gacgatccta
    16381 ttgaagctgc agcaatggct gccgaaaacg gtatttctat ctacaccatt ggcattggtt
    16441 cccctgaagg ggcgcctatt cccttaggga ataatcagga gttccttaaa gatgccgatg
    16501 gaaaggtagt tattacccga ttggatgagg aaaccctaac aaaaattgct gttaccgcta
    16561 acgggaagta tgttagggct tcaaataccc agtttggttt attgccaatt tatgagtcgc
    16621 ttaaaaaggt tgaccgtaga gcaattaagg ataaggttta ctccgagtac aatgagcagt
    16681 atcagtatat attggctatc gccattgcgc ttttatttgc tgaattaatt atccttgacc
    16741 gtaaaacttc gcttttatca aaggtaaacc tttttggtgc cgataaaaca agggaggata
    16801 tatgaggaaa gatattgtac ttatcattct tttagctgga ttcacggtta acacttttgc
    16861 tcaaagcgag cataagctaa tacgacgtgg taatagggca tatagtaaaa acgattattt
    16921 ggagtccgaa gtgcaatacc gtgaagcact tgagagaaac aagcattcct tcaaggcaaa
    16981 cttcaacctt ggcgatgccc tctataagca aaacaaattc tctgattccg attcaatctt
    17041 taatggtata aacacatctg ctctttccga tgctgataag tcgatgattt attacaataa
    17101 agggaattcg ctcttcaagc aaaataagtt taaggaaagt gccgatgcct ataaaatggc
    17161 cctcaagtat aatcctaacg atattgacgc taagtataac ctttccgagg cgcttagaat
    17221 gatgcagaac cagcagaacc agaacaacaa tcagcagcaa aacaatagca acgatagtaa
    17281 ggataacaag gatcagaaag acgataaaaa gcaagacaaa aaggaagata aaaaggataa
    17341 taacaataaa caggacaatc aacagaataa ccagaataaa aataaccaac agcaacaaca
    17401 acccaagatt tccaaagagg atgcagagcg aatgctacag gcattacagc aacgcgaaaa
    17461 ggacattcag gataaactaa ataaaaaaca agccaagccg gtttcggggg cttcaatcaa
    17521 aaactggtaa accctatgaa acgataccta agcggaatac tattgctttt catcagtggt
    17581 tacttacagt cccaggaagt aaccttacag gcatacgcac caaatctggt tgaagtgggt
    17641 gagcagttca gcttatcgtt tactttaaat gctaaaccat cagagtttaa ggctccctct
    17701 atagttgatt tcgatatttt agctggccct agcacatcca gcagcacaag tattgaggtg
    17761 attaacggta aagttaccca aagtcaatcc tatacctaca cctatatact tgttgcaagc
    17821 aaggagggaa aatatacgat tgatcctgca gaggctgttg tgggcaagga gcgtattcgc
    17881 tcaaatccca taacaattga ggtggtaaaa acaacatcag gaaatgctgc aagcagagcc
    17941 agccaacccg catctcgaaa cgttagggaa actcaaaccg acgatttgcc gggcgatgaa
    18001 ctatttgtaa ccattgagct cacccggaaa tcagcctacg taggtgaacc cattgaagca
    18061 gtgataaaga tatacacccg ggtaagcatt atggcttttg aagatgctaa atttcctgct
    18121 ttcgatggat tctggagcca ggagattaaa agttccccaa atgtaaactt tgagcgcgcc
    18181 aatgtgaatg gcaaaatata taatgccggc gtaattcgcc gttacctact tttccctcag
    18241 aaagctgata aaattgaaat tgatcccttt gaactggtag tggtttatca ggggcacaga
    18301 tctggcccac gctccatttt cgacgagttt tttggaggag gtttcgaaac ataccgcaag
    18361 cgtctagtga gtaagccggt tagcataaat atcaaaaaac ttccgttgcc tgaaccgcaa
    18421 gggtttacag gtgcagttgg tagcttcaaa attgatgctt cggttgataa gaacaccctt
    18481 aaagctaacg atgcttttac ctacaggctt aaaatatcag ggaaaggaaa ccttaagcta
    18541 attgggaacc caaaacttaa tttcccctca acatttgaag tttttgaccc aaaaatttcc
    18601 gagaacattt cagttgatga gggtaatgca atgggtagca aaacttacga gtatgtttgc
    18661 ataccaagag ctggcggtaa tttcagcata gaaccggctg tgttttctta ttttgaccct
    18721 gataaggaat cttatattac ccttaaaaca tcagctatct ctgttgccgt aagtgccgat
    18781 tcgtcaagca ctggcaatgt tatggttgca agttttggca aggaagatat caagtttatc
    18841 gggaaagata ttcgttttat taaaacatca agctctgctt tcaaatcaaa atctgcattt
    18901 tgggttacgt cgggttatta tcaacttcta tacatattac taattgcatt attcattaca
    18961 ttttcgtatc tattccgtcg ctatcgccag cgaatgcaag atgtagcctt ggttagaacc
    19021 cggagggcaa gcaatgttgc ccagaaacga ctcaaaatgg cgtcgcaact acttgagcag
    19081 aacaataatg cccagttctt tgaggagatc cataaagcta tttgggggta tgtgtccgat
    19141 aaactcaata ttcctttggc atcgctaagc ctcgataatg catcggagaa gctaacccaa
    19201 cagggtgttg atgccgacag aatttctact ttccgttcca tagttgagac ttgcgaatat
    19261 gcacgttttg ccccaatggc agagcattcg caaatgactg atatctacga gaagacattt
    19321 tcgctaattg aggatctgga agatactcta aaacgtaagt aaatgtgaca ttaaaaactt
    19381 tcaggaaatg aaacgcattc tatacttagc attaatgata gttcctgtta gcctgtttgg
    19441 aaaggtaaca atgcaaaatc ccgattcact tttcgcattg gctaataaat actacactga
    19501 ggggaaatat tccgaagctg ttacccatta tgaagctatt gtggcaaacg gaaaggtgag
    19561 cgctgagctt tacttcaacc ttgggaatgc ctatttcaag ctaaacgata tagcacacgc
    19621 cattctcaat ttcgagaggg cttacctgct aaaccctggc gatgacgata tctcttttaa
    19681 cctcgaacta gcccgtgcct acactactga caagattgag gctatccctg attttttcgt
    19741 ggttaagtgg gtaaagtcca tttccaacct atttagctct aacacttggg cttttcttgc
    19801 tatttcatca tttataattg cactgatttt atttatgatt tttcagttct ctggtaaata
    19861 tttaataaag aaatacgctt ttatcatttc atggttttct gcttttattt tcattgtttc
    19921 acttgctttt agtgtttcgc aaaaaaatag aattgtaaat tccaaccacg ctattgtaac
    19981 ttcctccgcc gtttcggtaa aaagttctcc ttcggagagt tcaaatgacc ttttcattct
    20041 ccacgcagga acaaaggtcg agaccttacg caatgttggc gagtggtgcg agattcgaat
    20101 tgccgatggc aataagggat ggttacccaa atcaacattt gaaagaatat aattctggca
    20161 ataggtaagg aaaaggtgga tgcctgtttt aattattagc gcgctctttg gggtgctctg
    20221 aaaaaagttt tgcaaaacct taaa
//
LOCUS       DAOOWJ010000042        20211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2659_length_20211_cov_7.272276, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000042 DAOOWJ010000000
VERSION     DAOOWJ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2659_length_20211_cov_7.272276"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLW22_05810"
     CDS             <1..655
                     /locus_tag="PLW22_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU74254.1"
                     /translation="PIAELKALLGEIPLLVDASQSAGHIPIEVDTWNIDMLALTGHKG
                     LMGPTGTGCLFVRTPEIINPLIHGGTGSRSDSFLMPDFLPDRFEAGTPNVLGIFGLYG
                     ALSANVTNSYERKHFMDLLERVEHLRSFKVFRSLDVKHQASLFSITSNKYSPSELSDQ
                     LFKRYSIETRSGLHCAPLAHQTLRTFPMGTVRFSLSKYHNSTDLNYLYSALKELDHG"
     gene            881..1921
                     /gene="selD"
                     /locus_tag="PLW22_05815"
     CDS             881..1921
                     /gene="selD"
                     /locus_tag="PLW22_05815"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOU74255.1"
                     /translation="MSTNFDLLTTVEYGGCSAKIPASVLESILLNLPKVTDPNVLVDI
                     DTHDDAGVFKINEDLALIFTTDFFPPVCSDPYEYGQIAATNSLSDVYAMGGEPFLALN
                     IAMFPSTKIPLDAYRQILEGGASVAQKANTIIIGGHTIDDYPPKYGLAVVGRVHPDKV
                     ITNAGLQPGDALILTKPLGTGVAVAAKRLGIVSEAAYSEALFWMKQLNNTAAQVMQTV
                     GVKGATDITGFGLIGHVYKMAKASRVGVKLYTSKIPLLPEVYQLVDEGCIPGAAFRNL
                     EFVGSHLTGDPNVDYNIKMLMCDAQTSGGILMGVKPERVNEALSLLKEAGLTYSAIVG
                     EVFEGDNILITS"
     gene            2033..2926
                     /locus_tag="PLW22_05820"
     CDS             2033..2926
                     /locus_tag="PLW22_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74256.1"
                     /translation="MIFREGEWYNFKITKVIDVPGGGKQFVLSHESGKKLLLPAQYYD
                     KYNIAENSTIQCRIDKINCTGQIFIEPAHPVYKTGGTYTFKIKHIGDTAIEEVQSIIV
                     CDIFGNEIEVLAPKGTIDDRQEEVKLKVLSVKKGVPILSAIAKWDICPDTLSNGQEIL
                     LFLSKVVVIQNEEYYLLKSKSNCIALLKVKHYKHYGFKEGIPIVARYRGTDNRGFLMI
                     DPEHPYYKVGGVYQFEIAAIEEDYSNELGSSRVAVVYDKFGMKCGLQIENASSFSVGK
                     SVSCKVIGYKKGRPLLEIVRM"
     gene            complement(2942..3271)
                     /locus_tag="PLW22_05825"
     CDS             complement(2942..3271)
                     /locus_tag="PLW22_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74257.1"
                     /translation="MSKKRAAVSLSNLSPELLKIFKERYPHGYQDFVFKVEKPNGDFF
                     HAVTLETEDAIYLVKVPVKIDTKIKDEDEEKEFFGSLTDDTIGTEGDEFPDDISDEEP
                     AEEIADE"
     gene            3450..3914
                     /locus_tag="PLW22_05830"
     CDS             3450..3914
                     /locus_tag="PLW22_05830"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU74258.1"
                     /translation="MKRLLLLNLLILLGLMANAQVNIYNPNADAKAEIAAAINKAKQE
                     NKHVFIQIGGNWCPWCVKLHNFMNADSTISEILTKNYVVVKVNYSKENKNLDVLEKLE
                     KPQRFGFPVLVILNSNGNRIHTQDSGLLESGDGYDRKKVLNFLKNWTPEALK"
     gene            complement(3995..5302)
                     /locus_tag="PLW22_05835"
     CDS             complement(3995..5302)
                     /locus_tag="PLW22_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74259.1"
                     /translation="MKRLTILISMLFVAVMVTAQTSKEINFDQYAKKVEKSNAEIQDP
                     KKNTKDATWIKRGELMIDLFDAQILRAYVGMDPNTFMLVVGKPIQQEKVEVDGVQLDK
                     YVMERVNFYFNNGQLERWEVTKPVIDKPLDLALESFLKAIEIDTKGKSTKKLQENLVK
                     LKGLYVNEGSNQYTLKDYKGAFESFKKVIEIGTLPILNQKDTAIYYYAGLSAQLAGLL
                     EESIPYYQKSIELGFTNDGAVYFNIFDAYKNLNKGDEGLKYLEEGFIKYPKNTNVLFS
                     LISYYIDKQEDPTKILVYIDKAIETEPNNSSLYFAKGTLYDRLGDGENALEAYKKAIE
                     IDPNFFDAYYNIGALYFNKGVKYIEEANKVPARELDKYDALMEKANAEFKNSMPYMEQ
                     AYKVNPNSKEAVEALKNIYFRFRNESQEMLDKYNEFNEKAKQM"
     gene            complement(5323..7821)
                     /gene="gyrA"
                     /locus_tag="PLW22_05840"
     CDS             complement(5323..7821)
                     /gene="gyrA"
                     /locus_tag="PLW22_05840"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOU74260.1"
                     /translation="MSEGERIIQINIEEEMKSSYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMADLGLNAGKPFKKSARIVGEVLGKYHPHGDSSVYEAMVRMAQDWAMRYPLV
                     DGQGNFGSVDGDSPAAMRYTEARLTRIAEETLADIDKNTVDFKLNFDDTLEEPTVLPT
                     KIPLLLVNGTSGIAVGMATNMPPHNLCEIVDAICATVDNPEITIDELLKYVKGPDFPT
                     GGIIYGYQGVKEAYLSGKGRVVIRSRTEIEQTESGRSKIIVTEIPYMVNKAEMIKKIA
                     DLINDKKIEGISYINDESDRQGTRVVIILKKEAVANVVLNNLFKHTQLQSSFAINNIA
                     LVDGRPKQLNLKDLIVQFIKHRHSIILRRTQYELDEAQKRAHILEGLLIAIDHIDEVI
                     NLIRAASSPNEAKEKLMERFGLSEVQAQAIIDMRLRSLTGLEREKVKQEYEELLKQIA
                     YLKQVLEDENLQKKIIKDEMIEMKEKYGDNRKTEIVPDAEEFNPEDFYADEEVVITIS
                     HMGYIKRTPLYEYKLQSRGGVGSKGSTTRDEDFIEHIYVASMHNTMLFFTEKGKCYWL
                     KVYEIPEGNKASKGRAIQNLINIENDDTIRAYINVKTLTDPEYLNNNYIVLCTRNGII
                     KKTSLEAYSRPRQSGVMAITIKKGDQLIEACLTNGNKQIILAAREGKAIRFHEKLVRP
                     IGRTGSGVRGMNLAQGDSIIGMICVEENENKDILVVAENGYGKRSKLDDYRVTGRGGK
                     GVKTITITPKTGKLISIKAVDDNNDIMIINKSGLTIRLSAKELRVIGRTAQGVKLINL
                     RKNDSIAAIANVEASDEENIDQQETIDNDESVNE"
     gene            8010..10553
                     /locus_tag="PLW22_05845"
     CDS             8010..10553
                     /locus_tag="PLW22_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOU74261.1"
                     /translation="MEQKFSQKIKDVIAYSREEAIRLGNTAITPDHLFLGILRDGEGE
                     AINILLSEGLNLNDLKKSIDSRLLQPADNNSGIDESEDISIYKSTERVLKIVILEARS
                     LKSDTINTAHLLLAILKDETSYITQLLLEHNVDYETVRKHLIVEDSGDKDDFSGGGSE
                     DDEGEGGIFGNMGRSGQSSSKSSDTPVLDNFGIDLTKAAEEGRLDPIIGREREIERIA
                     QILSRRKKNNPILIGEPGVGKSAIAEGLALRIVKKKVSRVLFNKRVIALDLASIVAGT
                     KYRGQFEERMKAILNELTRSPNIILFIDEIHTIVGAGGATGSLDAANMLKPALARGEL
                     QCIGATTLDEYREHIEKDGALERRFQKVMIEPTTPEETIEILNNIKERYEEHHNVTYT
                     PDAIEACVKLTMRYISDRHLPDKAIDALDEAGSRVHISNIKVPESILKLEKLIEETKK
                     EKIKAVKNQMFELAAKYRDNEKKLIEQLEAEQARWEKELTKHRETVDAEKVAEVVAMM
                     TGVPVQRIAQAEGARLLKMADELKRSVIGQDEAIDKIVKAIQRNRAGLKDPNRPIGSF
                     IFLGPTGVGKTQLAKVLAKYLFDTTDNLIRIDMSEYMEKFSVSRLVGAPPGYIGYEEG
                     GQLTEKVRRKPYSVVLLDEVEKAHPDVFHILLQVLDEGQLTDSLGRRVDFKNTIIIMT
                     SNIGTRDLKEFGQGVGFATKAKSEAKSDYEKSIIQKALKKAFAPEFLNRIDDIVMFNS
                     LTKSDIHKIIDIELKGLFDRVAGLGYTIKISTAAKDFITEKGYDVQYGARPLKRAIQK
                     YLEDPLAEALIKSDAKPGAVFLVGYSKKNDEITIRTAKAEEEVTNADEE"
     gene            10664..11170
                     /gene="tpx"
                     /locus_tag="PLW22_05850"
     CDS             10664..11170
                     /gene="tpx"
                     /locus_tag="PLW22_05850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOU74262.1"
                     /translation="MKTVTFGGNPVTLTGEQVKVGDKALEFTALDNSLKPVKMSDFKG
                     KVKLISIFPSIDTSVCSVQNHKFNKTASEFGDKVVFIAISNDLPFALKRFCGAEGITN
                     LVTLSDHRDLEFGTNYGFLIKELRLLARGVVIIDKDDTIKYVEYVPEIAQEPNYDAAI
                     KVLKELVK"
     gene            complement(11220..12515)
                     /gene="rimO"
                     /locus_tag="PLW22_05855"
     CDS             complement(11220..12515)
                     /gene="rimO"
                     /locus_tag="PLW22_05855"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOU74263.1"
                     /translation="MTKKINVVTLGCSKNVVDSEVLMHQLDRGGWKIVHNSNDPSARV
                     VVINTCGFIGDAKEESVETILGFVDAKNRGLIDKVYVMGCLSQRYRNELETEIPEVDG
                     YFGVSDLPQIVAELNVKYNEAELNKRWLSTPRHYAYLKISEGCNWGCSFCAIPLIRGK
                     HVSRPIESLVNEAKELAKQGVKELIVIAQDTTYYGFDIYGERRIAQLLNSLSQIDGFE
                     WIRLHYAYPSGFPDDLIDVLKSNPKICKYIDIPLQHINTQVLKMMRRGINREQTIDFI
                     EKIRKNIPEIVIRTTFLVGHPGETPEAFNELIDFVKDYRFDRVGVFAYSEEEGTYAAQ
                     NFTDSIPDEEKTRRVEKLMEVQQRVSFELNQLRVGHTYKVVIDAIEDHYAVCRTEFDS
                     PEVDQEVLVKLNAINVKPGEFHTVRIVEAQEYDLIGEFV"
     gene            complement(12522..13490)
                     /gene="ftsY"
                     /locus_tag="PLW22_05860"
     CDS             complement(12522..13490)
                     /gene="ftsY"
                     /locus_tag="PLW22_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019035538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOU74264.1"
                     /translation="MGILNIFNPDSKDNNNSSSLDQGLAKTKENLFKRISRAVAGKSR
                     VDDEVLDELEEGLISADVGVETTLRIIERIQKRAAAEKYLNTAELNTILKTEILSLLA
                     ENSENSILNNSGEIPKPYVIMVVGVNGVGKTTTIGKLAYMFKNQGKRVLLGAADTFRA
                     AAVEQLTIWANRVDVPIVKQQMGADPAAVAFDTLNSAKSNDTDVVIIDTAGRLHNKVN
                     LMNELSKIRRVMQKVIPDAPHEVLLVLDGSTGQNAFAQAREFTSATDVTAIALTKLDG
                     TAKGGVVLGISDQFKIPVKYIGVGEKMTDLQIFDRVAFVESLFRNV"
     gene            complement(13580..13732)
                     /locus_tag="PLW22_05865"
     CDS             complement(13580..13732)
                     /locus_tag="PLW22_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HOU74265.1"
                     /translation="MAKKVVASLQKATKDRVKCIKMVRSPKTGAYMFKEETMDKSESA
                     DFFKQK"
     gene            complement(13744..13926)
                     /gene="rpmG"
                     /locus_tag="PLW22_05870"
     CDS             complement(13744..13926)
                     /gene="rpmG"
                     /locus_tag="PLW22_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOU74266.1"
                     /translation="MAKKGNRVQVILECTEHKESGLPGTSRYITTKNKKNTPDRLERR
                     KYNPILKKVTLHREIK"
     gene            complement(13946..14179)
                     /gene="rpmB"
                     /locus_tag="PLW22_05875"
     CDS             complement(13946..14179)
                     /gene="rpmB"
                     /locus_tag="PLW22_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOU74267.1"
                     /translation="MSRVCQLTGKRVMVGNNVSHSKRRTKRRFLPNLKTKRLFLESEN
                     RWVTLRLSTSAMRTIDKVGLDNALKKFAKKGHI"
     gene            complement(14379..15623)
                     /locus_tag="PLW22_05880"
     CDS             complement(14379..15623)
                     /locus_tag="PLW22_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOU74268.1"
                     /translation="MRASILTIGDELLIGQVIDTNSAWLADSLSELGFEVTKILSISD
                     SSQAIAEAVNELMGQSDLVISTGGLGPTSDDRTKPTLCELFGGQLMLHAPSLSLIEQI
                     FHKRSLPLTETNKNQALIPSSCTPILNYHGTAPGMIFRKGNCLLISLPGVPFEMKNMF
                     TEGVRPFIEQNFTLPAIVRVTVNTFGIPESFLSDKLKDFEIYLADRSIALAYLPSPSG
                     IRLRLTATGENKGRLSNLLDMAVNELKSLIGENIFGFNDETLPSVVSKRLREKNATLA
                     VAESCTGGYISHLITQLPGSSDIYKGGIVAYSNEIKQNVLGVSHDLLISHGAVSQPVV
                     EAMLDGTLRVFQSEYAIAVSGIAGPTGATAGKPVGTTWIGVASRETRLVELYTFGNLR
                     EVNIQRAAYTALHQLLKLMDKD"
     gene            complement(15673..15746)
                     /locus_tag="PLW22_05885"
     tRNA            complement(15673..15746)
                     /locus_tag="PLW22_05885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15710..15712),aa:Thr,seq:cgt)
     gene            complement(15886..16869)
                     /locus_tag="PLW22_05890"
     CDS             complement(15886..16869)
                     /locus_tag="PLW22_05890"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOU74269.1"
                     /translation="MWIKKFKWLVLLFSLTSCISYRYIDIEFYDSPQKSIVVPGENLL
                     VVENLHLRNTDTPIRELDWALDSVASNEATDALISLLKTSPWYSGITLFKHIYYRNDS
                     SRVVLPLSWQVIDSLSNVYNSKMVVSLEYLKVRPYYDTYPLWRGDFKEYYGFIQVLSY
                     TYWRIYNVETRKINLGQLLTDTLLWEKNDWIEVTPGNQLPGIFEACAFAGADAGEQFA
                     QLIAPQWQKDSRAIYVPNTNKEMSRAYQLAMHGNWLDAAAVWQRLSSSTSKELAAQAA
                     FNMALANEMLGKFQLSIEWLDFAKSKYPHLKFLKEYRVILQHRIENNKPQK"
     gene            complement(16975..17721)
                     /gene="fabG"
                     /locus_tag="PLW22_05895"
     CDS             complement(16975..17721)
                     /gene="fabG"
                     /locus_tag="PLW22_05895"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOU74270.1"
                     /translation="MKLLEGKTGLVTGAARGIGRAIAIAFAQHGANVAFTDIAYDDNM
                     KSLEQELTALGVKAKGYASDASDYDNSQKIVDEIAKDFGQIDILVNNAGITRDTLLMR
                     MTEEQWDLVIKVNLKSVFNLTKAVQKYMLKQKNGSIINMSSVVGVSGNAGQANYAASK
                     AGIIGFTKAVARELGSRNIRCNAIAPGFIITEMTHKLPDEVREDWIKKIPLQRGGTPD
                     DVANVCVFLGSDLSSYVSGQVINVCGAMNT"
     gene            17891..>20211
                     /locus_tag="PLW22_05900"
     CDS             17891..>20211
                     /locus_tag="PLW22_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOU74271.1"
                     /translation="MKVKIHIRVKLIIAVLTVTAVVFWASIAFLNKRVDRYTRTNIYE
                     YIDATSKTYTTQIKGEFDKQFALARTITYSFYDLEQRNPEDVKQNTLRLLKGIAENTP
                     DLLSVWASWELWALDRKWDKPYGRDRYTYLRENNELKQIFERLNETGDNPSSLYYKLK
                     TEKKEALVDPYWYIYAGSQKRVLETSVCVPLVVNNRFAALFGFDIELSVYQEMIKNLK
                     PYEGSYAILISNNGTIVAHPDSTLLGALADTVLTNGKQIINSVITNGKYSETVLEGKS
                     KYYLTFNSFTAGQAAERWSLGIFVPEKVINEQISTITSQVRIVMVIGLIVLAIVLWII
                     AYSITSPLVRATKVLGELSLGKIDPAKKIEIKTGDEIEDIASSINILIDSLFKTSQFA
                     REIGKGNLQAKYSKLSDDDVLGDALLEMRKSLEYAKKIDEERKAEENKNRWYNEGMAK
                     FAEILRHNNSNLNDFAYETIRNLTKYVDATIGAVFLINNDNPNDIFLELMATYAYERR
                     KYEEKVIRMGEGLVGRCAQEAETIYMTEIPKGYIKIASGLGQDEPTELLLVPMKINDE
                     VHGVIEIASFEPIEDYKIKFIEKIGESFAATISSVKINIRTAKLLEESRIKSEELASQ
                     EEEMRQNMEELQATQEESARKSAEMESLINALHSSSYVIEYDLNGNIITVNEAYLKLT
                     GQTEKDIVGTHHADNLEMTSEQKNAYQKFWQDLRNGMIKKETSKVKIGGKTYTFIETY
                     SPILDENRRVVKILKIAHNITDFIDENPEGKTK"
BASE COUNT         5933 a         4113 c         4173 g         5992 t
ORIGIN      
        1 gcctattgct gagctaaaag ctctactggg tgaaattcca ttgcttgtcg atgcaagtca
       61 gtcggcaggg catattccta ttgaggtgga tacctggaac atcgatatgc tggcattaac
      121 tgggcataag gggttgatgg gaccaaccgg aacaggttgc ctgtttgttc gcacccccga
      181 aataatcaac ccgttaatac atggaggtac cggttcccgt tccgatagct ttctgatgcc
      241 cgacttttta cccgataggt ttgaggccgg aaccccaaat gttttgggta ttttcggttt
      301 atatggagcc ctatcggcaa atgtaaccaa tagctacgag cgaaaacatt ttatggacct
      361 actcgaaagg gttgagcatt taagatcatt taaagtgttc cgatcccttg atgttaagca
      421 tcaggcatcg ttattctcaa taacttccaa taaatacagc ccttcagaac tgtcggatca
      481 gcttttcaag cgctactcca ttgaaacccg aagtggtctt cactgtgcac cgcttgcgca
      541 ccaaacccta aggacgttcc ctatgggtac tgtcaggttt tcgctttcca aataccataa
      601 ttcaaccgat ttgaattacc tttactctgc cctgaaagag ctcgatcatg gatgaacgga
      661 aaggtaaatg tttactcctt tttcagagta ttcataaggt aatgcaagct gagcatatta
      721 ttgtaggctt aggcgtgcag tatcaaataa ttccagtacc caaggagtac agctctgaat
      781 gtggtatgtg tattgaaatt gattgttctg atagtaataa tctcttagct gaattacaac
      841 taaatagatt atcgtttaac atagtaatag aagtagattc atgagtacca atttcgactt
      901 gttaaccact gtggagtacg gtggctgttc ggctaagatt ccagcttcgg tgctcgagtc
      961 cattctgctt aacctgccca aagtgaccga tcccaatgta ttggtagata ttgataccca
     1021 cgacgatgct ggggttttca agataaatga ggatttagcc ctaatattca ccaccgattt
     1081 tttcccacct gtatgtagcg atccgtatga gtatggacaa atagccgcaa ccaattcatt
     1141 gagcgatgtt tacgccatgg ggggcgaacc tttccttgcg ctaaacatag ccatgttccc
     1201 ttctactaaa attcctttag atgcctacag gcaaatcctt gagggtgggg caagtgtagc
     1261 tcaaaaagca aatacaataa ttattggtgg tcacaccatc gacgactatc ccccaaagta
     1321 tggtttagcc gtggtgggta gagttcatcc tgataaggtg attaccaatg ccggcttaca
     1381 acccggcgat gctttgatcc tgaccaaacc tttgggcact ggtgtagcgg tagccgctaa
     1441 gcgcttagga attgtgtccg aagcggctta cagcgaggcc cttttctgga tgaaacagct
     1501 taacaatact gccgcgcagg tcatgcaaac cgttggtgtt aaaggtgcta ccgatattac
     1561 cgggtttggg cttattggcc acgtatacaa aatggctaag gcaagccgcg ttggtgttaa
     1621 gctttacaca agcaaaatac ccctgcttcc tgaggtatat cagcttgtgg atgagggctg
     1681 cattcccggt gctgcattcc gtaatctcga gtttgtaggc agccatctga ctggtgaccc
     1741 aaatgttgac tacaatatta aaatgcttat gtgcgatgct caaacgtcgg gtggaatact
     1801 aatgggtgtt aagcctgaaa gggttaatga ggccctaagt cttttaaaag aggctggttt
     1861 aacttattcg gcaatagtag gagaggtttt tgaaggcgat aatattttga ttactagtta
     1921 gatattgttg tttctggaat tgatatgtta atatttttga atcaaacttt aattttatca
     1981 ttcttatcaa ttattttacg ataattcatt gtaaaacaac atactgtttt ttatgatttt
     2041 tagggaaggg gagtggtata attttaagat tactaaagta attgatgtgc ctggaggcgg
     2101 caagcaattc gttctttccc atgaatcggg caaaaaattg ctattgccgg ctcaatatta
     2161 tgataagtac aatatagcag aaaactctac cattcaatgc aggatagata agataaactg
     2221 cacgggtcaa atttttattg agccagcgca tccggtttac aaaaccgggg gtacatatac
     2281 ttttaagatt aaacatattg gcgatactgc aattgaggag gtgcagagta taattgtatg
     2341 cgatattttt ggtaatgaaa tagaagtttt agctcctaaa gggaccattg atgatagaca
     2401 agaggaggta aagcttaaag tgcttagtgt taaaaagggt gttcctatcc tttcagcaat
     2461 tgctaaatgg gacatttgcc ccgatacctt aagtaatgga caggagatat tgctttttct
     2521 aagtaaggta gtagttattc agaacgagga gtattacctg ctaaaatcaa aatcgaattg
     2581 tatagccttg ctaaaggtaa agcactataa acactatggt ttcaaagaag ggattcctat
     2641 tgttgccaga tatagaggta ctgataacag aggatttttg atgattgacc cagaacatcc
     2701 atactataag gtgggtggag tatatcaatt tgagattgca gcaattgaag aggattatag
     2761 taatgaacta ggtagtagta gggttgcggt agtgtatgat aaatttggca tgaagtgtgg
     2821 cttacaaatt gagaatgcct catcgttcag tgtaggaaaa agcgttagtt gcaaagttat
     2881 agggtacaaa aaaggacgac ctctactcga aatcgtccgc atgtagctta aattaagaaa
     2941 attactcgtc ggctatctcc tcagcaggct cctcatcgct aatatcatca gggaattcat
     3001 cgccctcggt tccgatggta tcatcggtca agctgccgaa aaattccttc tcctcatcct
     3061 catcctttat ctttgtgtca atttttactg gtacctttac caggtaaatg gcatcctcgg
     3121 tttcaagggt aacggcatgg aaaaaatcgc catttggctt ctcaaccttg aaaacaaagt
     3181 cctgataccc atgggggtat ctttccttga atatttttaa aagctcagga gacaaattgc
     3241 taagacttac tgctgctctt ttcttactca taaatcgctt aaaattttcc gcaattatag
     3301 tgcaaaaaaa gaaattgcaa ggggagttgt gaatattttt ttcaaaaaat attatataac
     3361 acaatatcag agtgttaaaa taggattttt tgcttttgaa tatccgattc caatatctta
     3421 atatctttga gtgttctaaa ttaaatacta tgaaacgatt gttgctacta aatctattaa
     3481 ttttattggg attgatggct aacgcccagg taaacatata taatcccaat gccgacgcta
     3541 aggctgaaat tgcagccgca attaacaaag caaagcagga aaataagcat gttttcattc
     3601 aaattggagg caattggtgt ccatggtgcg ttaagctcca taattttatg aacgcagatt
     3661 caactatatc cgaaatccta accaaaaact acgttgtggt taaggttaac tacagcaagg
     3721 aaaataaaaa tttagatgtt cttgaaaagc ttgaaaagcc acagcgtttt ggttttcctg
     3781 ttcttgtgat tttaaattcc aatggaaaca gaatacacac tcaggattcg ggtttgctag
     3841 agtccggcga tggttacgac cgaaagaaag ttctgaattt ccttaagaac tggactccag
     3901 aagcacttaa ataaaagtca tataattaat attatgttaa gtaatatgct ttaaataaaa
     3961 gccccacttc ggggctttta ttaatttatc agaattacat ctgttttgcc ttttcgttga
     4021 attcattgta cttatccaac atttcctggc tctcgttacg gaaacggaaa tagatatttt
     4081 tcaaagcttc aacagcttcc ttgctattgg gatttacttt atatgcttgt tccatgtaag
     4141 gcattgagtt cttaaattct gcattagcct tttccataag agcatcgtat ttatcaagtt
     4201 cacgagcagg aaccttattg gcttcctcta tgtactttac acctttattg aagtaaagcg
     4261 caccaatatt gtagtaagca tcaaagaaat taggatcgat ctcaatagct tttttgtagg
     4321 cttccagagc attttcgcca tctcccagcc tatcgtacaa tgtacccttt gcaaagtaaa
     4381 gcgatgagtt gtttggctcg gtttcaattg ccttatcaat gtatacaaga atcttagtag
     4441 gatcttcctg tttatcgata tagtaggaaa ttagagagaa taatacgttt gtgtttttgg
     4501 gatactttat gaaaccttcc tctaggtatt ttagaccttc atcgccttta ttcaggttct
     4561 tgtaggcatc aaatatatta aagtaaacag caccatcatt ggtaaaacca agttcaatgg
     4621 atttctggta gtatggaatt gattcctcaa gtaatccggc aagttgagct gataaacctg
     4681 catagtaata aattgcagtg tctttctggt tgagaatagg aagtgtacca atctctataa
     4741 cctttttgaa ggattcaaag gcacccttgt aatcttttag agtatactgg tttgaacctt
     4801 cgttaacgta tagacctttt agttttacaa ggttttcttg caatttcttg gtgctcttac
     4861 ccttggtgtc gatctcaatt gctttcagga aactttcaag ggccaaatcc aaaggtttat
     4921 caataacggg cttagtaacc tcccagcgtt caagttgacc gttgttaaag tagaagttaa
     4981 ccctttccat aacatactta tcgagttgaa ccccatcaac ttctactttt tcctgctgta
     5041 tgggttttcc tacaacaagc ataaatgtat ttggatccat tccaacgtat gccctgagga
     5101 tttgagcatc aaaaaggtct atcataagct cacccctttt aatccatgtg gcatccttgg
     5161 tattcttttt gggatcctgg atttcggcgt tgcttttttc aacctttttg gcatactgat
     5221 cgaaattgat ttccttgctg gtttgcgctg ttaccattac agcaacaaag agcattgaaa
     5281 ttaaaattgt tagtcgtttc ataagataat tgttaaatta agttactcgt ttactgattc
     5341 gtcgttatca atggtttctt gttgatcaat attttcttca tcgcttgcct ccacattggc
     5401 tatggccgca atggaatcgt ttttcctgag gttaattagt ttaacaccct gtgctgttct
     5461 gccaataact cgaagttctt tggcactcaa tcggatggtt aaaccgctct tgttaataat
     5521 cattatatcg ttattgtcgt caacggcttt aatgcttata agtttacctg tttttggtgt
     5581 aatagttatt gttttaacac cttttccacc tctaccggta actcggtaat cgtcaagttt
     5641 agaacgttta ccataaccgt tttctgctac aaccagtata tccttgtttt cattttcttc
     5701 aacacaaatc atgccaataa tggaatcgcc ctgtgctagg ttcattcccc taacgccact
     5761 tccggttcta cctattggac gtacaagttt ttcgtggaaa cgaatggctt ttccttcgcg
     5821 agcagctagg atgatttgct tgttaccatt ggttaggcat gcctctataa gctgatcgcc
     5881 ctttttgatg gtaattgcca ttactccact ctgtcgtgga cggctatacg cctctagcga
     5941 tgtcttttta attatgccat tccttgtgca aagaacaata tagttattat tcaggtactc
     6001 agggtcggtt aatgttttaa cattaatgta tgcacgtatt gtgtcgtcgt tctcaatgtt
     6061 aataaggttc tggatagccc tgccctttga agccttgtta ccttcaggta tttcgtacac
     6121 ttttaaccag taacacttgc ctttttcagt gaagaaaagc atggtgttat gcatggatgc
     6181 tacatagata tgctcaatga aatcctcatc gcgggttgtg ctaccttttg agcctactcc
     6241 ccccctggat tgaagcttgt attcatacag tggagtacgc ttgatataac ccatgtggga
     6301 aatggtgata accacttcct cgtcggcata aaaatcttcg gggttgaact cctcagcatc
     6361 aggcacaatc tcagttttac gattatcgcc atacttttcc ttcatctcga tcatctcatc
     6421 cttgatgatc tttttctgaa ggttttcatc ctctagaacc tgtttgagat aagcaatttg
     6481 ctttagtagt tcctcatatt cctgcttaac cttttcacgc tcaagcccgg taaggctcct
     6541 taagcgcata tcgatgatag cttgggcttg aacttcgcta agaccaaacc tttccataag
     6601 cttttccttg gcctcgttag gcgaagaagc agcacgtata aggttaatca cctcatcgat
     6661 atggtcaata gctattagta atccttccag gatatgggcc cgtttttgag cttcatctag
     6721 ctcatattga gtacggcgca ggataatgct atgcctgtgc ttaatgaact gtactattaa
     6781 atccttcagg ttaagctgct tggggcgtcc atccaccaaa gcaatattgt tgatagcaaa
     6841 agaggattgc agctgtgtgt gcttaaacaa attgtttaac acaacattgg caacagcctc
     6901 ttttttaagg attattacaa ctcgagttcc ttgcctgtcg gattcatcgt taatatagct
     6961 gataccctca attttcttat cgttaataag gtcggcaatc tttttaatca tttcggcctt
     7021 attcaccata tagggtattt ccgtaacaat aatcttggag cgcccggatt cggtttgttc
     7081 aatttccgtt ctggagcgga tcaccaccct accctttccg gaaaggtatg cttccttaac
     7141 tccctgataa ccatatatta taccacctgt tgggaaatcg ggtcctttaa catatttaag
     7201 cagttcatct atggtgatct cggggttatc cacagtggca caaatggcgt caacaatctc
     7261 gcagaggttg tggggaggca tgttggtggc catccctacg gctatacctg atgtgccgtt
     7321 gaccagaaga aggggtattt tggtaggaag caccgtgggt tcttccaggg tatcgtcgaa
     7381 gtttagctta aagtcaacgg tattcttgtc gatatcggca agtgtctcct ctgcaattct
     7441 ggttagccta gcctccgtgt aacgcatagc tgcgggacta tcgccgtcaa ctgatccaaa
     7501 gttaccctgt ccatcaacca gcgggtagcg cattgcccaa tcctgcgcca tgcgaaccat
     7561 tgcctcataa accgaggagt cgccatgagg gtggtactta cccaggacct cacccacgat
     7621 acgtgccgat ttcttgaatg gtttacctgc attgagcccc agatcggcca ttccgaaaag
     7681 aacccttctg tgaacgggtt ttaaaccgtc acgcacatcg ggtaatgctc tcgatactat
     7741 caccgacatt gaatagtcaa tgtacgatga tttcatttcc tcctcaatgt tgatctgtat
     7801 aatcctttca ccctcagaca tataataaaa gcttaaaaag tcatttttta cttcagaaat
     7861 aacgtgctaa gatagctatt taagcgtaaa aaaaagaatg gttaatgatg ttgttcatta
     7921 tttattaaaa aaatactaaa tttattaaca aaagcattgt tgttacgttt atgtctatta
     7981 ctttagtaaa agtttttgga gaagtattta tggaacaaaa gttttcacaa aaaatcaaag
     8041 atgttatagc ttacagtcgc gaggaggcta tccggttggg taatacagcc attacccccg
     8101 atcatctctt tttaggtatt cttcgtgatg gtgagggcga agcaatcaat attctgcttt
     8161 cggagggttt aaatttaaac gatctaaaaa agtcgattga cagccggttg ttacaacccg
     8221 ctgataataa ttcgggtatt gatgagtctg aggacattag tatttacaaa tcaactgaaa
     8281 gggttttaaa aatcgtaata cttgaagccc gttcgcttaa aagcgatact atcaatacag
     8341 cccatctttt actcgcaatt ctaaaggatg aaaccagcta tatcacccag ttgctactag
     8401 agcataatgt tgattacgaa acggttcgta aacatttgat tgtggaggat tcgggtgata
     8461 aagatgactt ttccggcggt ggcagcgagg atgatgaagg cgaaggtggt atttttggca
     8521 atatggggcg ttcaggtcaa tcatcctcta agtcgtccga taccccagta ttggataact
     8581 ttgggattga tcttaccaaa gccgcagagg agggtcgttt agacccaata atcggacgag
     8641 aaagggaaat agagcgaata gcccaaatac tgagccgccg aaaaaagaat aatccaatcc
     8701 tcattggtga gcctggcgtt ggtaaatcgg ctattgccga agggttagcc ctacgaattg
     8761 ttaaaaagaa agtgtcgcgt gtgctattca ataaacgggt aatcgcactc gatttagcat
     8821 caatagttgc cggcacaaag tatcggggac agtttgagga gcgcatgaaa gccatactaa
     8881 atgagcttac ccgtagccca aacattattc ttttcattga cgagattcat actattgtag
     8941 gagcaggtgg agccaccggc tcgctcgatg ccgccaacat gctcaaacct gcacttgccc
     9001 gtggcgaact ccaatgcatt ggggctacta cccttgacga gtaccgcgag cacattgaga
     9061 aggatggagc gttggagcga cgcttccaaa aggtaatgat tgaacccaca acgcctgagg
     9121 agacgattga aatactcaac aacattaagg agagatacga ggaacaccat aacgtaacct
     9181 atacccccga cgccattgag gcatgcgtga aacttaccat gcgatacatc agcgacaggc
     9241 atttgcccga taaggcaata gatgccctgg atgaggctgg ttcgcgcgtt catatctcaa
     9301 acattaaggt tcccgagagt attctcaagc tggaaaaact tattgaggag accaaaaagg
     9361 aaaagatcaa agcggttaag aaccagatgt ttgagctagc ggcaaagtat cgtgataacg
     9421 aaaaaaaact tattgaacag ttagaagcag agcaagcgcg gtgggaaaaa gagctaacta
     9481 agcatcgcga aactgtagat gccgaaaagg tagccgaagt tgttgccatg atgactggag
     9541 ttcccgttca gcgtatagct caggctgaag gcgcacgtct actcaaaatg gccgatgagc
     9601 tgaagcgctc agttattggt caggacgagg caattgataa gattgttaag gctattcaac
     9661 gtaaccgcgc cgggctaaag gatccaaatc gtcctattgg ttcgtttatt tttcttgggc
     9721 caaccggtgt tggaaaaaca cagcttgcaa aggttctggc aaaatacctt ttcgatacta
     9781 ccgataacct tattcgtatc gatatgagcg agtacatgga gaaattctcc gtgtcgcgac
     9841 ttgttggtgc gcctccggga tacataggtt acgaagaggg agggcagctc accgagaaag
     9901 tacgtcgaaa accctactcg gttgttttgc tcgatgaagt tgaaaaagca caccccgatg
     9961 tttttcacat tctactccag gttttggacg aaggccagct aaccgacagc ttaggacgta
    10021 gggttgattt taagaatacc ataataataa tgacctccaa cattggaacc cgtgatttga
    10081 aggagtttgg acaaggtgta ggttttgcca caaaagccaa gtcggaggca aaaagcgatt
    10141 acgagaaaag tataatccaa aaggcgctga aaaaagcctt tgcccccgaa ttcttaaacc
    10201 gtattgatga cattgttatg ttcaactcgc ttaccaagag cgatatacac aaaatcatcg
    10261 atattgagct taaaggatta ttcgacaggg ttgctggttt aggctatacc attaagattt
    10321 cgactgcagc caaggatttc atcacggaaa agggatatga tgttcagtat ggcgcgcgtc
    10381 cactaaaacg agccattcaa aagtatctgg aagatcctct agctgaagcc ttaataaaga
    10441 gcgatgcaaa acctggtgca gtattcctgg ttggctatag taaaaagaat gacgaaatta
    10501 ccattcgaac agcaaaagct gaagaggagg ttaccaacgc cgatgaagag taataagttg
    10561 taatacaatg agaaagagac gcacaacaaa aggtgtgtct cttttttttg aacattcccc
    10621 tagcaaagtt gttatattag tagcaaacta accaaataga attatgaaaa cagtaacatt
    10681 tggcggaaat cccgtaaccc tgacaggcga acaggttaag gtaggcgata aagctttaga
    10741 atttacagct cttgataact ctctaaagcc tgtaaaaatg agcgatttta agggaaaagt
    10801 aaaactaata tccattttcc catccattga caccagcgta tgttcggttc agaaccacaa
    10861 gttcaacaag accgcatcgg aatttggcga taaggttgta tttatagcga tttcgaacga
    10921 tttgcctttt gccctaaagc gtttttgtgg agctgaggga attaccaact tagttactct
    10981 ttcggatcat agagacctag agtttggtac aaactatggt tttctgataa aggaattaag
    11041 gctacttgcc cgtggtgttg tgataataga taaggatgat accattaaat acgtggagta
    11101 tgtacctgaa attgcccaag aaccaaacta cgatgctgca attaaagtat taaaggaatt
    11161 agttaagtag catgatgcat taaattgaaa taaaggtcgt taacaacgac ctttattttt
    11221 tatacaaact caccgattaa atcgtattcc tgggcctcta caatacgtac tgtatgaaac
    11281 tcgccgggtt ttacattaat agcgttaagc tttaccagaa cctcctgatc cacctcgggt
    11341 gaatcgaact ccgttcggca aacagcgtaa tggtcttcaa ttgcatcaat tacaacctta
    11401 tacgtatgac caactctcag ctggtttaac tcaaaagata ccctttgttg aacctccatc
    11461 agcttttcaa ctctacgtgt tttttcttca tcgggtatgg agtcggtaaa gttttgtgca
    11521 gcataggttc cttcctcctc agagtatgca aaaacaccta ccctgtcgaa ccggtaatcc
    11581 ttaacaaaat caatcagttc attgaaagcc tcaggggttt cccctgggtg gccaaccaga
    11641 aaagtggttc ggataacaat ttcggggata ttctttctta tcttctcaat gaaatcaatg
    11701 gtttgttctc ggtttatccc cctacgcatc attttaagta cctgagtatt tatatgctgt
    11761 aatggaatat cgatatactt gcaaattttc gggttcgact ttaggacatc aattaggtca
    11821 tcgggaaaac cagatgggta tgcatagtga agcctaatcc attcaaaacc gtcaatctgg
    11881 ctgaggctat tgagtagctg agcaattcgg cgttcaccat atatatcaaa tccgtaatag
    11941 gtagtgtcct gtgcaattac aattagttcc ttaacgcctt gtttggcgag ttcttttgcc
    12001 tcgttaacca atgattcaat ggggcgggaa acatgcttac ctctaataag gggtattgca
    12061 cagaatgaac atccccagtt gcaaccctcg gatattttca ggtaagcgta atgccttggg
    12121 gttgaaagcc agcgtttatt cagctcggct tcgttatact tcacattgag ctcagcaaca
    12181 atttgtggta aatcggaaac cccaaaatat ccatctactt cggggatttc agtttccagt
    12241 tcattgcggt agcgttgcga aaggcatccc ataacataaa ccttgtcgat cagcccccta
    12301 tttttggcat cgacaaagcc aagtatagtt tcaaccgatt cttccttagc atcgccaata
    12361 aaaccgcaag tgttaatcac aacaaccctt gccgatggat cgtttgaatt gtgaacaatt
    12421 ttccatcccc ctctatcgag ctgatgcata agcacctcgg agtcaacaac attttttgag
    12481 caccccaagg taacaacatt gatttttttc gtcatgtttg gctaaacgtt acgaaatagc
    12541 gattcaacaa acgcaaccct gtcaaatatc tgcaaatcag tcattttttc acccactccg
    12601 atatacttca cgggtatctt gaattgatcg gaaatgccaa gcacaacacc cccttttgcc
    12661 gtaccgtcga gcttggtgag agcaatggct gtaacatcgg ttgccgaagt gaactcgcgt
    12721 gcctgtgcaa aagcattttg acctgttgaa ccatccagaa cgagcaatac ctcatgtggt
    12781 gcatcgggaa taaccttttg cattacccta cgtattttgg aaagctcgtt cattaaattg
    12841 actttattgt gaagacggcc agcggtatcg ataattacta cgtcggtgtc gttagatttg
    12901 gcagagttta gggtgtcgaa agcaacggca gctggatcgg ctcccatttg ctgcttaaca
    12961 atgggaacat ctaccctatt agcccaaatg gtaagctgct caacggctgc cgctctgaag
    13021 gtatcggcag cacccaacaa tacccttttc ccctggttct taaacatgta tgccagtttg
    13081 ccaatagtag tagtttttcc aaccccgtta acgccaacta ccataattac atagggttta
    13141 ggaatttcac cactattatt aaggatagaa ttttcggaat tctcggcaag taacgatagt
    13201 atttcggttt taagtatagt atttaattct gctgtattca ggtacttttc ggcagcagca
    13261 cgcttttgga tgcgttcaat aatgcggaga gtggtttcaa cgccaacatc ggcggagata
    13321 agtccctcct caagctcgtc caagacctcg tcgtcaaccc tggacttacc ggctacagcc
    13381 cttgaaattc ttttgaaaag gttctccttt gttttggcca aaccctggtc aagggaactg
    13441 gagttattat tgtccttact atcgggatta aaaatgttaa gaatacccat atacaatatt
    13501 attttcgggc gctaaaataa caataaaagc ttttcgaata aaccgaaaag cttttatttg
    13561 taagatataa tgcagaaaac tatttctgtt tgaagaaatc ggccgattcg gatttatcca
    13621 ttgtttcttc cttgaacatg tatgcgcccg ttttgggtga acgcaccatt ttaatgcact
    13681 tcacacgatc tttcgtggct ttttgaagcg aagcaactac tttctttgcc atatctaaaa
    13741 gtgttattta atttcacgat gaagtgtaac cttcttgaga atcgggttgt acttacgacg
    13801 ctcaagccta tcgggtgtat tcttcttatt cttagtggta atataccttg aagtaccagg
    13861 taaaccactt tccttatgtt cggtacattc cagaataacc tgaactctat tacctttctt
    13921 tgccattgct atactttttt aaaaattaaa tgtgaccttt cttagcaaat ttcttcaggg
    13981 cattatctaa accaacctta tcgatggttc tcatagctga agtgctaagc ctaagggtaa
    14041 cccaacggtt ttcactttca aggaacaacc ttttggtttt aaggttaggc aggaatctgc
    14101 gtttggttct tctttttgaa tgcgaaacgt tgtttcccac cattactctt tttccggtta
    14161 actgacaaac tcttgacatc gctgtatatt ttaccgtttt tacttttttc gaaacaggac
    14221 gcaaattacg tcattattta tgaattattc aaatattttg ataaaaaaaa ttgctgaaaa
    14281 ttagcttaat ttagatacct tctcatttaa tgatgaaatt acaattacca tatttatatt
    14341 atatacattt atttacgaag aatatttaaa ctgatacact aatccttatc cataagtttt
    14401 agcaattggt gcaaagccgt atatgctgcg cgttgaatat taacctccct gaggtttcca
    14461 aaagtataga gttctaccag tcgcgtttcc cttgatgcaa caccaatcca ggtggtgcct
    14521 acaggcttac cggcggttgc tcctgttggt ccggcgatgc cggacactgc aatggcatac
    14581 tcactctgga aaactcttag tgtaccatcg agcatggcct caactactgg ttggctaact
    14641 gcgccatgac ttatgagcaa atcatggctt acccctagca cgttttgttt aatctcgttt
    14701 gagtatgcta caatacctcc cttatatata tcggagctac cgggcagctg agttattaaa
    14761 tgcgaaatat agccacctgt acagctttcg gccactgcta gggttgcatt tttttcccta
    14821 agtcttttag acactaccga gggaagtgtt tcgtcattga aaccaaaaat attttcacca
    14881 atcaaactct taagttcatt tactgccata tccaacaagt tgcttagcct acccttattt
    14941 tcaccagttg cagtaagacg taacctgatt cccgaagggc ttggcaggta ggctagcgca
    15001 atacttctat cagctaaata tatttcaaag tcttttaact tatcggaaag gaacgattca
    15061 gggattccaa aggtgttgac ggttacccga actattgcgg gaagggtaaa attttgctca
    15121 ataaatggcc gaaccccctc ggtaaacata ttcttcattt caaatggaac tcccggaagc
    15181 gaaatcagaa ggcaattgcc tttcctgaat atcattccag gagccgttcc atgatagttg
    15241 agaattggtg tgcacgaaga gggaattaat gcttggttct tattggtctc agttaaagga
    15301 agactgcgtt tgtgaaaaat ctgttcaatt aggcttaggg agggtgcatg gagcataagc
    15361 tgaccaccaa atagctcaca gagcgttggc tttgtccggt cgtcgctagt agggcctaac
    15421 ccgcctgtgg aaataacaag atccgactgg cccattagtt cgttaacagc ttcggcaatt
    15481 gcttgtgagc tatcggaaat gctaaggatt ttggttacct caaacccaag ctcgctaagg
    15541 ctatcggcca accaggccga attggtatcg ataacctgac cgataagtaa ctcatcgcca
    15601 atggttagga ttgatgctct catttgcttg caccactaaa tttaaaaagg acaggttgcc
    15661 ctgtcctacc agagcctctc acgggacttg aacccgcgac ctgcggttta cgaaaccgcc
    15721 gctctaacca actgagctaa agaggcatta ttaccgctgc aaaaatatca attagataac
    15781 aattcgcaaa acaggctata ggatagaaat aaaaataatt tataattaat tgatataatg
    15841 aagtttgcca ctttctactt tacgagtaat tatagaatat taagcttact tttggggttt
    15901 attgttctct attcggtgct gcaaaataac cctatactcc tttagaaact taaggtgagg
    15961 gtatttagat tttgcaaagt cgagccattc aatggatagc tggaatttgc ccagcatctc
    16021 gttggctagg gccatattga atgctgcttg tgcagccaac tccttactgg ttgatgaaga
    16081 taacctttgc caaaccgcgg cggcatctaa ccagtttcca tgcatggcaa gttggtatgc
    16141 tctgctcatc tccttgttag tgttgggaac atatattgct ctgctgtcct tttgccattg
    16201 cggagcaata agctgtgcga attgctcgcc ggcatcggct ccagcaaatg cacaagcctc
    16261 gaatataccc ggaagctggt tgccaggtgt aacctcaatc caatcgtttt tttcccataa
    16321 aagggtatcg gtaagtaact ggcctaaatt aatctttctt gtttcaacat tgtaaatgcg
    16381 ccaataggta tagcttaata cctgaatgaa tccgtaatat tccttaaaat cgccacgcca
    16441 caaagggtat gtatcatagt atggacgcac ttttaagtac tcaagcgaga caaccatttt
    16501 actattgtaa acattactga ggctatcgat tacttgccag gaaaggggaa gaacaaccct
    16561 tgaagaatcg ttacggtaat aaatatgttt aaaaagggtt ataccggagt accatggaga
    16621 agtttttaat aggcttatga gggcatcggt tgcttcattt gaagcaacgc tatccaaagc
    16681 ccaatcaagt tcccgaatgg gagtatcggt atttcgtagg tgaagatttt caaccactag
    16741 taggttttca cccggaacaa caatcgattt ttggggtgaa tcgtaaaact caatatcaat
    16801 atacctatat gatatacaag atgttaatga gaaaagtaat actaaccatt taaacttttt
    16861 tatccacata aacttttaat agggttttgg catttcataa aataaaaaag ttgaaaacaa
    16921 gcaacttaag tattagtatg cctgctttca acttaaatta taatgctaca agtactaggt
    16981 attcattgca ccacaaacat taataacctg tccgctaacg tacgacgata gatcggaacc
    17041 caggaataca caaacattgg ctacatcgtc gggggtacca ccacgctgaa ggggtatttt
    17101 ctttatccaa tcctcgcgaa cttcatcggg taacttgtgg gtcatttcgg tgataatgaa
    17161 tccgggagca attgcgttgc aacggatgtt acgtgagccc agctcgcggg caacagcttt
    17221 ggtaaatcca attattccag cctttgacgc tgcatagttg gcctgtccgg cattacctga
    17281 aactccaacc accgagctca tgttaataat tgaaccgttt ttctgcttaa gcatatactt
    17341 ttgaacggcc ttggtaaggt taaaaaccga ctttaggtta accttaatca ctaaatccca
    17401 ttgctcctcg gtcatacgca taagcaatgt atcgcgagtg ataccggcat tgttgaccag
    17461 gatatcgatt tggccaaaat ctttggctat ttcatcaaca attttctggg aattatcgta
    17521 atcgctagca tcggaagcgt atcctttggc ttttacgcct aatgcggtta gctcctgctc
    17581 aagcgatttc atgttgtcat cgtaagcaat gtcggtaaaa gctacatttg ctccatgttg
    17641 agcaaaggca attgcaatgg cgcgccctat accccgagcc gcacctgtaa ccaagccagt
    17701 ctttccttca agtaacttca taggtttaat ttttgaatga ttttcaaacg caaatgtaat
    17761 aaatattggt aattaaactt ctgttttaag tatcaaagct cacttattgc tcctattatt
    17821 gcttgtttat ttcaaaagtg ctatattcac acattaaaag ttgattgttt ttcttcaaaa
    17881 aacatttacc atgaaggtga aaattcatat tagagtaaag ttgattatcg ctgtacttac
    17941 ggttacagca gttgtttttt gggcaagcat tgctttttta aacaaaaggg ttgataggta
    18001 tactcggaca aacatttatg aatacattga tgccacgtca aaaacctaca ccacccaaat
    18061 taagggtgaa tttgataagc agtttgcctt agcaagaacc ataacctata gcttttatga
    18121 ccttgaacaa cgaaatcccg aagatgttaa gcaaaatact ttaaggctac taaaaggaat
    18181 tgcagaaaat acccctgatt tactgtcggt ttgggcaagc tgggaacttt gggccttaga
    18241 taggaaatgg gataaacctt acggcagaga taggtacacc tacctgaggg aaaacaatga
    18301 acttaaacaa atatttgaaa gattaaacga gaccggtgat aacccaagta gtttgtacta
    18361 taaacttaaa accgaaaaaa aagaagcact ggttgatcca tactggtaca tctatgccgg
    18421 aagtcaaaaa agggtactgg agaccagcgt atgcgtgccg ttggtagtca acaacagatt
    18481 tgctgcgctc tttggctttg acattgagct ctcagtttat caggaaatga taaagaatct
    18541 aaaaccctac gaagggagtt acgccattct aatttcgaat aacggtacca ttgttgcgca
    18601 tcccgattcc acattgcttg gtgcactggc cgatacggtt ttgactaacg gaaaacaaat
    18661 aattaacagc gtaattacca atgggaaata ctcagaaaca gtgttagagg gaaaaagtaa
    18721 gtactacctt acctttaact cttttactgc aggtcaagct gctgaaaggt ggagccttgg
    18781 tatattcgta cccgagaaag taattaatga acaaataagc accataacaa gccaggttag
    18841 gattgtaatg gtaataggat taatagtgct agcaattgtg ctttggatta ttgcctacag
    18901 cattacaagc ccattagtaa gagcaacaaa agttctaggt gaactctccc ttggtaagat
    18961 tgacccagcc aaaaagattg agataaagac tggcgatgag attgaggata ttgcaagttc
    19021 aattaacatt ttaattgaca gtttgtttaa aacctcgcag tttgccaggg aaataggtaa
    19081 aggtaattta caagcaaaat actcaaaact atccgatgac gacgttttag gtgatgcgct
    19141 acttgaaatg cgtaagagcc tggagtatgc taaaaagatt gatgaggaaa ggaaagctga
    19201 ggaaaacaag aatagatggt acaacgaggg catggctaag tttgctgaaa tattgaggca
    19261 caataacagc aacctaaacg actttgctta cgaaaccatt cgaaacctaa caaagtatgt
    19321 tgatgccaca attggtgctg tatttcttat caataatgat aatccaaatg atatcttcct
    19381 tgagttgatg gcaacctatg cctacgaacg ccgaaaatac gaggagaaag tcattcgaat
    19441 gggtgaaggc cttgttggac gatgcgcgca ggaagccgaa acaatatata tgaccgaaat
    19501 acctaagggg tatataaaaa ttgcctcagg gcttggtcag gacgaaccca ccgaattact
    19561 tcttgtcccc atgaagatta acgatgaagt tcacggggtt attgagattg cctcgtttga
    19621 gcctattgag gattataaaa ttaaatttat tgaaaagata ggcgaaagtt ttgctgcaac
    19681 catatcgagt gttaaaataa acattaggac tgcaaaatta cttgaagaat caaggataaa
    19741 atcggaggag ctcgcatcgc aggaggaaga gatgcgacaa aacatggaag agcttcaggc
    19801 aacccaggag gaatcggctc gcaagtcggc cgagatggaa agcctaatca atgctttgca
    19861 ttcttccagc tacgttattg agtacgattt gaatggcaac attatcactg taaacgaagc
    19921 ctaccttaaa ctaactgggc aaaccgagaa agatattgtt ggaactcacc atgccgacaa
    19981 cctggagatg acctccgaac agaaaaatgc ctaccaaaag ttctggcagg acctacgcaa
    20041 tggaatgatt aaaaaggaaa catctaaggt taagattggc ggtaaaacct atacattcat
    20101 tgagacctac tcacctattc ttgatgaaaa cagaagggtt gtgaagattc tgaagatagc
    20161 tcacaacata accgatttta ttgatgaaaa tccagagggg aaaacaaaga a
//
LOCUS       DAOOWJ010000043        20191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2666_length_20191_cov_8.040723, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000043 DAOOWJ010000000
VERSION     DAOOWJ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 20191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20191
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2666_length_20191_cov_8.040723"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1243)
                     /locus_tag="PLW22_05905"
     CDS             complement(80..1243)
                     /locus_tag="PLW22_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU74272.1"
                     /translation="MEQSASFFSFLLDNLTQFLSFTGFSNFTVGHGIMIVVGLLFIYL
                     AIAKEFEPMLLVPIGFGIIVGNIPFTPGYQIGVYESGSVLNYLYFGVLKGIYPPLIFL
                     GIGAMTDFSALISNPKLMLIGAAAQLGIFGAYMLALLLGFSPQEAGAIGIIGGADGPT
                     AIFLSSKLAPELMGAIAISAYSYMALVPVIQPPVMKLLTTQKERLIRMKPPRQVSKLE
                     KIMFPIIGMLLTTFIVPSGLPLLGMLFFGNLIKESTVTKRLADTAKGPMIDIVTILIG
                     LTVGASTQATTFLTPKSVGIFALGAASFVVATFGGVMFVKFLNLFLKEGNKINPLIGN
                     AGVSAVPDSARVSQVVGLEYDKTNHLLMHAMGPNVAGVIGSAVAAGILLSFLA"
     gene            complement(1246..1683)
                     /locus_tag="PLW22_05910"
     CDS             complement(1246..1683)
                     /locus_tag="PLW22_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU74273.1"
                     /translation="MKDFKFKINGNEYVVHISNVDGNIAELEVNGTPYRVELDKELKQ
                     TKTPKLIRPEAVPATDSHPAVAKTVSPGSATSGAVKSPLPGVILDVLVKPGDHVKVGQ
                     RLIVLEAMKMENNIDSDKEGKVVEVKVRKGDSVLEGDTLIIIG"
     gene            complement(1708..2616)
                     /locus_tag="PLW22_05915"
     CDS             complement(1708..2616)
                     /locus_tag="PLW22_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOU74274.1"
                     /translation="MKRLILATVLVVLGFVSVNAQSVYDLRINEVMLTNKTNYIDSYG
                     ERSAWIEIVNTGYNSVNVAGCFLTNDISNPMKYRIPSADPITLIPQQQYLVFFADGKS
                     VHGTLHLNFTLDSINRFVALIAPDGSTLIDSVTLPAIGIDKSFVRLPNGKGEWRISEL
                     ATPNSTNELFETHVSTGERFVKFDPFGVIMAIIAMSVVFTALLVLYRLFKLTARLVQK
                     PIRIEFRKSKVAKAEAVEEVAEEVPGEVFAAISAALYFYETEKHDHESTVLTIERVSR
                     RYSPWSSKLYGLQQTPNRIIVHRKLN"
     gene            complement(2643..4205)
                     /locus_tag="PLW22_05920"
     CDS             complement(2643..4205)
                     /locus_tag="PLW22_05920"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOU74275.1"
                     /translation="MSTQEQKIKELIDLREKAKLGGGPKRIEAQHKKGKYTARERIDM
                     LLDEGSFEEFDMFVSHRCIDFGLDKESYLSDGVVTGYGTIDGRLVYVFSQDFTVFGGS
                     LSEMFAAKICKIMDMAMKVGAPVIGINDSGGARIQEGVKSLGGYADIFQRNILASGVI
                     PQISAIFGPCAGGAVYSPALTDFIIMSRNTSYMFVTGPKVVKTVTGETVSSEELGGAG
                     VHSTKSGVAHFVSETEEEGIMLIRKLLSYLPQNNLEEPPLMPCNDPIDRLEDALNEII
                     PDSPNKPYDVKEVIGAIVDNGEFLELQRDYAPNIVTCFARFNGQSVGIVANQPKYLAG
                     VLDINASRKAARFVRFCDAFNIPIVTLVDVPGFLPGTGQEYNGIIIHGAKLLYAYGEA
                     TVPKVTVILRKAYGGAYDVMSSKHLRGDINYAWPSAEIAVMGPKGAIEVLLSRELETI
                     EDEKAKVEFLLRKEQEYKEKFANPYVAAKFGYLDDVIEPRNTRFRIIRALQSLATKKD
                     TNPPKKHSNLPL"
     gene            complement(4234..4644)
                     /gene="mce"
                     /locus_tag="PLW22_05925"
     CDS             complement(4234..4644)
                     /gene="mce"
                     /locus_tag="PLW22_05925"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOU74276.1"
                     /translation="MSISHIEHIGIAVKSLEESIPFYEKVLGLKCYNIEEVKDQKVKT
                     AFFLVGQTKIELLESTDPEGPIGKFIEKKGEGIHHIAFAVKDIEEQLKRMEEQGVTLI
                     DKAPRKGAEGLDIAFLHPKSTLGVLTELCEDKNK"
     gene            4844..4969
                     /locus_tag="PLW22_05930"
     CDS             4844..4969
                     /locus_tag="PLW22_05930"
                     /inference="COORDINATES: protein motif:HMM:NF028380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HOU74277.1"
                     /translation="MGKGDKKTRRGKIFLGSFGNTRPKKSKVRKEKRDKAKVQAK"
     assembly_gap    5021..5120
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5283..7448
                     /locus_tag="PLW22_05935"
     CDS             5283..7448
                     /locus_tag="PLW22_05935"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74278.1"
                     /translation="MSLLIILLISGSTYSQNTQYDRLTFQRDSLFASQLSNQIDSLFY
                     AGQYDSCMLIIDKAIIFFDKLGLTRYKIDALTRRGSIYRINGKFSNAMADFGDVLEYY
                     QRTGNTKGEASTLNHIGAIYRLRGDYPTALDYYFKALANYQKINYLPGISSVLNNIGV
                     VHLYQKIYDKALEYYNKSLSIEETLKSDEGIGTSYLNIGEVYRKKGDLQKAADYYLRA
                     LVYANRTNDLDGIGTIYNELAGINIENGSTDDVLKYLRLAYNTFLSTNSPQRLAECQI
                     NFGKYYQAIGNINQAIKHYSQAFELADKNKLLEIKSDAHRQLSTLFDKQGNTVLAYSH
                     FKKYIETRDSLFNEDNTRKSIQAEFFYKFERQQEEHRIAEAKKEAEYAERLRREKVVK
                     TSLITIISLGAILIGFILFYLNKIKLKNEELKYHQKEILEKNEELQQQQEEILAQRDE
                     IERKNVILEQSQQIIADKNERMISSIEYARTIQNALLPKPEKFSELFSDYFVIYQPKD
                     IVSGDFYWISSDERYSYAAVMDCTGHGVPGAFMSMIGNTLLNKIVIEWGVNQPSKVLE
                     ALNEQLREALKQKEVGNIVLAGIDIAFVTIDKHERKISFSGAARPMLIIQNGEMQLVK
                     GSIRSTGGFQPANTKPFDDACFDLLSPTNIYLFSDGYSDQISIDKKKFGLQRFTELIY
                     QSHSKPMAAQRDLLIQMMENHLHGADQLDDICVMGLRLA"
     gene            complement(7451..9424)
                     /locus_tag="PLW22_05940"
     CDS             complement(7451..9424)
                     /locus_tag="PLW22_05940"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU74279.1"
                     /translation="MHEDLYAPFDFPVYKTEQELFAEKSKLLKEFKSFYRYDSTIHPQ
                     QLEGFRSSFDLAFDKFINSQNVSKTSRAKYLKVKPELEQFVADNISQIYLKGIINQDE
                     SAISNSMLKQGLAVVINNVAYDKWFDEVYTVQKAVKLLNSNANDFTKSLSPGLVGLWE
                     MINLKEYIQPNLFYDEVATQKFRNELISNISITKGLVQAGEKIVVKGEVVNSEIYQIL
                     ESLKKEYDKRLGSKNLFAILFGNMILIASLYIVLFLFLLNFRNEVLNDIRKLLFILML
                     ITASAAVSALVIKTNSFSIFVIPLAIVPIFVKTFFDSRLALFVHLITIFLVAFLVPNS
                     FEFVFTNFIAGIVAIIGLSKMYNRGQLFKTVGLVYFSYLTLTLSLYLVGDGSLKGVNW
                     FIALWYGINALLLLASYQLVYIIEKFFGFLSDITLIELSDTNLDLLRKLAEVAPGTFQ
                     HSLQVANLAESAVQRVGGNPLLVRTGALYHDIGKMANPMFFIENQQGGFNPHENVDNV
                     KSAEIIINHVHEGVKIAAKAGLPSQIVDFIKMHHGTSKVKYFYNKELQAENANIDEGK
                     FSYPGPEPQTKETAIVMMADAVEAASRSLKEYNVQSIDQLVERIIDDQLNSGQYSFAD
                     VTLRDISLIKTIFKKKLQNIYHVRLEYPDLSIR"
     gene            complement(9585..11024)
                     /locus_tag="PLW22_05945"
     CDS             complement(9585..11024)
                     /locus_tag="PLW22_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU74280.1"
                     /translation="MKEYLKSNIFRVISDVAEIEGVDAYVIGGFVRDILLNRPSKDID
                     IVVVGSGVDFARKVAQRLRARKVTYFKNFGTAQLKHNEWDIEFVGARKESYRLDSRKP
                     IVENGSLEDDQKRRDFTMNALAIGLNKRNFGELIDPFNGLQDLKSKTIRTPLDPATTF
                     SDDPLRMLRAIRFATQLNFDIELKTLDAIREMSSRITIVSIERVVDEINKMMASPRPS
                     VGWLLLEQTGLLQHIFPELAAMKGVEARNGVSHKDNFYHTLEVLDNLSLKSDNLWLRW
                     AALLHDIGKPKTKRFVAGQGWTFHGHEFLGAKMIPEIFRKLKLPLNDKMKYVQKMVLL
                     HLRPIVLSQEVVTDSAVRRLLFDAGDDIDDLMMLCEADITSKNDITVRKHLANFRLVR
                     KKLKEVEEKDSIRNFQPPVTGEMIMETFNLKPCKEVGLIKNRIKDAILDGEIRNDFFE
                     AYTLMLNAGKELGLTAININPQYISLTNK"
     gene            11242..11598
                     /gene="trxA"
                     /locus_tag="PLW22_05950"
     CDS             11242..11598
                     /gene="trxA"
                     /locus_tag="PLW22_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU74281.1"
                     /translation="MIEHLTAETFKQKVFDYEKNNEWKFEGDKPAIIDFYADWCGPCK
                     MVAPILEELAKEYEGKLNIYKVDTEEEQELAAVFGIRSIPSLLFIPLNDQPQMAMGAL
                     PKDTFKKAFKDVLGVE"
     gene            complement(11833..>12312)
                     /locus_tag="PLW22_05955"
     CDS             complement(11833..>12312)
                     /locus_tag="PLW22_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOU74282.1"
                     /translation="KVITLSDRASRGEYEDLSGPEVAKLVSDFFNSVSWQLEVETEII
                     PDDRDLLENRLIEARNSKYDMVLTTGGTGIGPRDITPDVVKPLLDKEIPGIMEHIRLK
                     YGAEKPNALLSRGVAGLMGTTFVYTLPGSVKAVREYMGEISKVQKHLFLMLMGIDSH"
     assembly_gap    12313..12412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12413..12600)
                     /locus_tag="PLW22_05960"
     CDS             complement(<12413..12600)
                     /locus_tag="PLW22_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74283.1"
                     /translation="MEQTNPITVKVVSVNISEKKGTTKHAVDRIELNSLGVKGDAHAG
                     AWHRQVSMLGVESIDKFT"
     gene            complement(12603..13055)
                     /gene="moaC"
                     /locus_tag="PLW22_05965"
     CDS             complement(12603..13055)
                     /gene="moaC"
                     /locus_tag="PLW22_05965"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOU74284.1"
                     /translation="MELSHTDKNGKANMVDVGHKPNQIREASATGFIRLLPQTVKLIR
                     ENQMKKGDVLTIAEIAGIQAAKRTPELIPLCHTLMLTKVDVKATLCENGVEVKSYAKC
                     IGQTGVEMEALTAATVALLTIYDMCKAVDKQMVIERVELIEKAKKDIV"
     gene            complement(13057..13857)
                     /locus_tag="PLW22_05970"
     CDS             complement(13057..13857)
                     /locus_tag="PLW22_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU74285.1"
                     /translation="MFDQFNRRINYLRISVTDRCNLRCTYCMPEEGIVQIPHNQVLTF
                     EEIVEIARIAVQHGFDKVRITGGEPLVRKGIVDLVRMLKTVEGITDLGLTTNGTLLKQ
                     FAQPLYDAGLKRINVSLDTLDPQKYKAITRVGTLSDVLEGLEEAKKVGFNPIKLNCVV
                     ENNSNEPDAQAVKAFANSNGFIARFIPKMNLDDGVFGVVEGGEGGNCSTCNRLRLTAD
                     GILKPCLFSDKGYNVRELGIQQAIEMAIKNKPACGVANRSGEFYNIGG"
     gene            complement(13841..15022)
                     /locus_tag="PLW22_05975"
     CDS             complement(13841..15022)
                     /locus_tag="PLW22_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOU74286.1"
                     /translation="MISLDKAIRIVEQSVTALNGQEHVSLSESLNRVLRHDVVSDINM
                     PPFRKTAVDGYACRAADLDNPLTVIEVIAAGTIPQRTIAQGTCSKIMTGAPIPNGADC
                     VIPVEDTEILSDGTIRFKGSKPKSNICELGEDIKIGESPLSKGTLIKPQHIAIMAALG
                     CHSPIVSVRPRVSILPTGDELVEPTEIPQGSKIRNSNGHQLIAQVISAGAIPMYHGIV
                     GDTEEVTEKAIARALAESDVVVLTGGVSMGDFDYVPRIMERLGVKILFDSIAVQPGKP
                     TTFGVAGNKLIFGLPGNPVSSFVQFELLVKPALLMMMQCNEPYARTYKLPLAIDYSRK
                     RAERLGLFPVFVNDIGEVEPLEFHGSAHIFSLAKATGIAAIPLGVKELKKGELLDVRP
                     I"
     gene            16301..18286
                     /locus_tag="PLW22_05980"
     CDS             16301..18286
                     /locus_tag="PLW22_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOU74287.1"
                     /translation="MSHEPVEKIKGLPENAYTELREGEEYQPVMPPSKAVPEVTAWSV
                     IWGLIMAIVFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSTLAKRKGALGENVIIQSIG
                     ASSGVIVAGAIFTLPALYILNLEAEFYQVFLSSLFGGILGILFLIPFRKYFVADMHGK
                     FPFPEATATTEVLVSGEKGGKQARLLVVSGLIGGIYDFIIATFGWWSEVVTTRVIPAG
                     EALAEKAKLVFKLNVGAAVMGIGYIIGLKYTAIIAAGSFVGWFVIIPIINYFAPGLTE
                     AVGSNITLTVGQMSAEQIFSNYVRPIGIGGIAMAGIIGIIRSSGIIRKAFGLAVSELT
                     GKHSAIPKEELRTRRDLPMSIIAGGILFTTILLLVFFYFGVVHNITHALIALGIVMVI
                     AFLFTTVAANAIAIVGTNPVSGMTLMTLIITSLVMASAGLSGTSGMIAALIIGGVVCT
                     ALSMAGGFITDLKIGYWLGSSPYKQQTWKFLGTFVSAATVGGVIILLNKTYGFTGKDA
                     LVAPQANAMAAVIEPMMSGKPAPWALYAAGAFLSLILTMIGVPALAFSLGMFIPLQLN
                     TPLLVGGIIAHIVSTRSKDNSLNRARKERGTLIASGFIAGGALMGVVSALLRFGGINY
                     VNPEWFESHSAEILGFVMFLILCGYLIWESLRAKVEE"
     gene            18351..18737
                     /locus_tag="PLW22_05985"
     CDS             18351..18737
                     /locus_tag="PLW22_05985"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU74288.1"
                     /translation="MIDYKQLDVWIASRQLANSIYTISKYFPKDEIFGLTQQIRKSAI
                     LIPSNIAEGIGRNHLKETTQFLYIARGSLFELETQIYIAFDQGYIDATALEQITNDIE
                     KCKKLLNGFINYYLKKKHEDHTNTKN"
     gene            18769..20007
                     /gene="pepT"
                     /locus_tag="PLW22_05990"
     CDS             18769..20007
                     /gene="pepT"
                     /locus_tag="PLW22_05990"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOU74289.1"
                     /translation="MINKQSLIDRFIRYVKIDTQSDDTVTERFPSTEKQLVLSNLLVK
                     ELKELGAQEVEIDQYGYVMATIPANTSKKVPVLGFLAHVDTSPDMPGKDVNPRFVENY
                     DGSEIILNKELGIALSPKEFPELKNYVGQTLIVTDGTTLLGADDKAGLAEIMTAVEYL
                     IKNPSFEHGKIRIGFTVDEEVGRGVDHFDVKRFGADFAYTLDGGAIGELEFENFNAAS
                     AKISIQGRNIHPGYAKDKMLNAILIAQEFNSLLPAHERPEHTQGYEGFYHLIKMDGSV
                     EKAAFQYIIRDHDRKKFEARKAYMQRIAEYLNTKYGEGTVTLELKDQYYNMREKIEPV
                     YHVVSTAMEAMKAAGVNPKVKPIRGGTDGARLSYMGLPCPNLFAGGENFHGKHEYVAV
                     ESMVKATEVMLKIIELYASK"
BASE COUNT         5823 a         4407 c         4076 g         5685 t
ORIGIN      
        1 gctgcattgc agaacaaaaa aagttgaaaa aacaaaaaag ccccgttgcc ggggctttat
       61 atcactggat acttatgtgt taggcaagaa agctaagcag gataccagca gcaacagcgc
      121 taccaataac acctgcaaca ttgggcccca tggcatgcat gagtagatgg tttgttttat
      181 cgtactccaa acctactact tgcgatacac gggcactatc aggaaccgca gaaacacccg
      241 cattaccaat taatgggtta atcttattcc cctctttaag gaataggttt aaaaacttaa
      301 caaacatcac tccaccaaag gttgccacca caaacgaggc tgcgccaagc gcaaaaatac
      361 caaccgattt aggggtaagg aaagtagtgg cctgggttga tgcaccaaca gttaaaccaa
      421 taagaatggt tactatatca atcattgggc ccttggctgt atcggccaaa cgcttggtaa
      481 cagtactttc ctttattagg tttccaaaga agagcattcc cagcaatggt aagcctgagg
      541 gtacaatgaa agtggtgagc agcattccaa taattggaaa cataattttt tcaagctttg
      601 aaacctgtct gggtggtttc attcggatta aacgctcttt ttgtgtagta aggagtttca
      661 tcacgggtgg ctggattaca ggcaccaacg ccatgtatga gtatgccgat atggctatag
      721 cacccataag ctccggggct aactttgacg ataggaagat tgccgttggt ccatcggcac
      781 caccaataat accaatcgca ccagcctctt gcggcgaaaa gccaagtaac agggcaagca
      841 tataggcccc aaaaataccc agctgagcag cagctccaat taacataagc ttggggtttg
      901 aaattagcgc actaaagtcg gtcatagccc ctatacctaa gaatatcagc ggtggataaa
      961 ttcctttaag tacaccaaag tataggtagt ttaaaacgct tccactttca taaactccta
     1021 tctgataacc cggagtgaat gggatatttc caacaataat accaaaccca attggaacca
     1081 gaagcatggg ctcaaactcc ttggcaatgg ctaagtaaat aaacaacaac cccacaacaa
     1141 tcataatacc gtgaccaaca gtgaagttgg aaaaaccggt aaaggataaa aactgggtta
     1201 agttatccaa caggaacgag aaaaaacttg cgctttgctc catggctaac caattattat
     1261 aagtgtatca ccttcaagaa ccgaatcgcc tttacgaacc ttaacctcaa caacttttcc
     1321 ttccttatcg gaatcgatgt tgttttccat tttcatggct tcaagtacta tcaagcgctg
     1381 tcccactttc acatgatcac ccggcttaac aagcacatcg agtataacgc ctggcagggg
     1441 cgatttaaca gcacccgagg tagctgaacc agggctaact gtcttagcta ctgcgggatg
     1501 cgagtcggtt gcaggcacag cctcaggtcg aataagttta ggcgttttag tttgcttaag
     1561 ctccttatcg agctccaccc tgtatggcgt tccattcact tcgagttctg ctatgttccc
     1621 gtcaacattc gagatgtgaa ctacatactc gttgccattt atcttgaact taaaatcttt
     1681 cattttgagt gattgttagc gttttaacta attcaatttc ctatgaacaa taatgcgatt
     1741 tggagtttgt tgcaaaccat aaagttttga actccaaggc gaatagcggc gcgaaacccg
     1801 ctcaatggta agtacagttg attcatggtc gtgcttttcg gtttcataga agtatagcgc
     1861 agctgatatg gctgcaaaaa cctcacccgg aacctcttca gccacttctt ccactgcctc
     1921 tgccttggca actttagact ttcggaattc aatgcgaata ggcttttgaa ccaacctggc
     1981 agtcaactta aaaagacggt atagcactag taaagcagtg aacacaaccg acattgctat
     2041 gattgccata attacgccaa aggggtcaaa ctttacaaac ctttcaccag ttgatacatg
     2101 agtctcaaaa agttcattgg tagagtttgg agtagcaagt tcgctaatcc gccattcccc
     2161 ttttccgtta ggcaatcgta caaatgactt atctatacca attgcaggta aggtaacact
     2221 atcaatcaga gtactgccat cgggtgcaat aagtgccaca aaacggttga tgctgtcaag
     2281 cgtaaaattc aagtgtaatg taccatgaac acttttacca tcggcaaaaa acaccaaata
     2341 ctgctgttgc ggtataagcg tgatgggatc agccgatgga atacggtatt tcatggggtt
     2401 tgagatgtcg ttggtaagga aacaccccgc aacgttaaca ctattgtagc ccgtatttac
     2461 tatctcaatc catgcacttc gctcaccata gctatcaatg tagttcgttt tgttagttaa
     2521 catcacctcg ttaatgcgga ggtcgtaaac gctttgtgcg tttacgctaa caaaccctag
     2581 aactacaagg actgtagcca aaatcaatct tttcattttc atcattgcta caatcatttt
     2641 ggttacaacg gtaggttaga gtgtttcttt ggtgggttgg tatccttttt ggttgctaac
     2701 gactgtaacg cccttattat gcggaagcgg gtgttgcgcg gttcaatcac atcgtcgagg
     2761 taaccaaact tggctgctac gtatgggtta gcaaactttt ccttgtactc ctgttccttg
     2821 cgaagcagga actcaacctt agctttttca tcctcaatgg tttccaactc acggctaagt
     2881 aatacctcaa tggcaccctt gggtcccata accgctattt cagcacttgg ccaagcgtag
     2941 ttgatatcgc cacgcaggtg ctttgagctc atcacgtcgt aagcgccgcc gtaggcttta
     3001 cgtaatatta cagtaacctt aggaacagta gcctcgccgt aggcgtaaag aagttttgct
     3061 ccatgtatta tgataccatt gtactcctgg ccggtacctg gcaaaaagcc cggaacatca
     3121 acaagtgtaa ctataggaat attgaatgca tcgcagaacc ttacaaagcg agcagctttg
     3181 cgggaagcat tgatatcgag cacaccggca aggtatttgg gttggttggc cacaattccc
     3241 accgactgtc cgttgaaacg ggcaaaacag gttacaatat tgggtgcata gtcgcgctgc
     3301 aactccaaga actcgccatt atcaacaata gctccaatta cttcttttac atcgtatggc
     3361 ttgtttgggc tatcggggat aatctcgttc agggcatcct ccaaccgatc gataggatcg
     3421 ttgcagggca ttagcggtgg ctcctcaagg ttgttttggg gcaggtagct caagagcttg
     3481 cggataagca taatgccttc ctcctcggtt tcggatacaa aatgtgcaac ccccgatttt
     3541 gtggaatgaa ccccagcacc accaagttcc tcggatgaaa cagtctcacc ggtcacagtt
     3601 ttaaccacct ttggaccagt aacaaacatg tagctggtat tacgcgacat gatgatgaag
     3661 tcagtaagag cgggtgaata aaccgcccca ccggcgcatg gaccaaaaat ggctgatatc
     3721 tggggtataa cacccgaggc taaaatgtta cgctgaaaaa tatcggcata tccgcctaaa
     3781 ctttttactc cttcctgaat tcgagcgccg ccgctatcgt tgataccgat taccggagca
     3841 ccaactttca tggccatatc catgatcttg cagattttag cggcaaacat ctccgatagt
     3901 gagcctccaa acactgtaaa atcttgtgaa aatacgtaaa ccaatcggcc atcaatggtg
     3961 ccgtagccgg ttactacacc gtcagatagg tagctctcct tatcgagacc aaaatcgata
     4021 cagcggtgcg aaacaaacat atcgaactct tcaaaactac cctcatcgag cagcatatcg
     4081 atacgctcgc gtgctgtgta ttttcctttt ttatgctgag cctcaatacg cttggggcct
     4141 ccgccaagtt ttgctttttc ccttaaatca ataagttctt tgattttttg ctcctgtgtg
     4201 ctcatcgtta tatttggtgt ttggggatgt tttttactta tttttatcct cgcaaagctc
     4261 agttaatacg cccagggttg acttcggatg aaggaaagca atatcaagcc cttcagcacc
     4321 cttacgggga gctttatcga taagggttac tccctgctcc tccatgcgct ttaactgctc
     4381 ctcaatatct tttacagcaa aagcaatatg gtggataccc tcaccctttt tctcaatgaa
     4441 ttttcctatt ggcccttcag ggtctgtaga ttcaagcaat tctattttgg tctggccaac
     4501 caggaaaaag gctgtcttaa ccttctgatc ctttacctct tcaatattgt aacactttaa
     4561 acccagtacc ttttcgtaga aaggaatcga ttcctcaaga cttttaacgg ctataccgat
     4621 gtgttcaatg tgtgatatgc tcatagctgt attattttaa attagtttgt gcaaagttac
     4681 tttgtatagt ttactttaac cataaaagtt tcaaacgttt tagttgtttt tcagcattgt
     4741 taaagaacat gtttgttaga attttgccga aaatggaaca taaaataaaa taatcgctta
     4801 tttttgcagc caactttgag taataaacta atactataac accatgggaa aaggcgataa
     4861 aaaaacccgc aggggtaaaa tatttttggg ttcgtttggc aacacccgcc caaaaaaatc
     4921 aaaagttcgt aaggaaaaac gcgataaggc taaagtccaa gcaaagtaaa gcgaacttac
     4981 taacgaatga ctcaaaccgg cacagccggt tttttttatt nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn ttatcacctc aaaccaacac tatgttaacg catagtgcaa
     5161 tttaatcaaa aattgacacc aattattagt tgcaataaga gaaaaaagta aataaatggc
     5221 taaatttgat taatagtcgt gaaagtgtaa tgccgtaccc taaagctaaa actttcaaca
     5281 ttttgtcact actgattata ttgctaatca gcggttctac ttattcccaa aacactcagt
     5341 acgatcgctt aacctttcag cgcgattcct tgtttgcctc gcagctttcc aaccaaattg
     5401 atagcttatt ctatgccgga cagtacgata gctgtatgct gataattgac aaggcaataa
     5461 tctttttcga taagttaggc ttaaccaggt ataaaattga tgccttaacc cgtaggggat
     5521 caatttacag aattaatggt aagtttagta acgccatggc cgattttggc gatgtgctgg
     5581 agtattacca acgaaccggg aatacaaagg gcgaagcctc cacgctaaac catattggtg
     5641 caatttaccg gctaagaggc gattatccca cagcgcttga ctactacttt aaggctttgg
     5701 ccaattacca gaaaattaac tacctgcctg gcatttcctc ggtcctcaac aatattggag
     5761 ttgttcattt gtaccaaaaa atctacgata aggctttaga atactacaac aaatcgttat
     5821 ccattgagga aaccctaaag agcgatgaag ggattggtac ttcgtaccta aacattggcg
     5881 aagtttaccg aaaaaaaggc gatttgcaaa aggccgccga ttactacctc agagctttag
     5941 tttacgctaa ccgtacaaat gaccttgatg gtattggaac catttacaat gagctggcag
     6001 gcattaacat cgaaaacggc tccactgacg atgttttgaa gtacttaagg ttagcttaca
     6061 atactttttt aagtacaaac agtcctcaac gacttgcgga gtgccaaata aactttggga
     6121 aatactacca ggctataggc aacatcaatc aagccataaa gcattacagc caagcctttg
     6181 aacttgccga taaaaataaa ctgcttgaaa ttaaaagcga tgcccatcgt cagctaagta
     6241 cactgttcga caagcaaggc aatactgtat tggcatactc ccacttcaaa aagtatattg
     6301 aaacccgcga tagcctattt aacgaggata acacccgaaa atccatacaa gcggaattct
     6361 tttacaagtt tgagcgccaa caggaagaac accggattgc cgaagccaaa aaagaagcgg
     6421 aatacgctga acgtttaagg cgcgaaaaag tggtcaaaac ttcacttatc acaataatta
     6481 gtctaggcgc tattctaatt gggttcattc tattctatct aaataaaatc aaattaaaaa
     6541 acgaggagct caaataccac caaaaggaaa tccttgaaaa aaacgaggag ctccaacagc
     6601 aacaggaaga aattttggcc caacgcgacg agattgagcg aaaaaacgta atccttgaac
     6661 aatcgcaaca gataatagcc gataagaacg aaaggatgat tagcagtatt gagtatgcca
     6721 gaacaatcca aaacgcattg ctaccaaaac ccgaaaaatt ttcggaattg ttttcggact
     6781 actttgttat ataccaaccc aaggacattg tgagcggcga cttttactgg attagcagcg
     6841 atgagcgtta ctcctatgcc gcagttatgg actgtacagg gcatggtgtg cccggcgctt
     6901 tcatgtcgat gattggaaac accttgctta acaagattgt gattgagtgg ggcgttaacc
     6961 aaccatcaaa ggttcttgag gcgcttaacg agcagctgcg cgaagccctg aaacaaaaag
     7021 aggtgggtaa tattgttttg gcgggtattg atattgcctt tgtaaccatc gataagcatg
     7081 aaaggaaaat ctccttctca ggcgcagcac gacccatgct aatcatacaa aatggcgaga
     7141 tgcaactggt aaaaggcagc atccgctcaa ctggcggttt ccaaccggca aacaccaagc
     7201 cctttgatga tgcttgcttt gatctacttt cgccaacaaa tatctaccta ttttccgatg
     7261 gttactccga tcaaatcagt atcgataaaa agaaatttgg cttacaacgt tttactgaac
     7321 ttatctatca atcgcatagc aaacccatgg ctgcacaacg cgacctgcta atccaaatga
     7381 tggagaacca tttacatggt gctgaccaac ttgatgatat ctgcgtgatg ggactccggt
     7441 tagcatgatg ctacctgata cttaaatcgg ggtactccag ccgaacatgg taaatattct
     7501 gcaacttctt cttaaaaatt gtctttatca gcgaaatatc cctaagcgta acatcggcaa
     7561 agctgtactg ccctgagtta agctgatcat cgataatcct ctcgaccaac tgatcgatac
     7621 tttgtacgtt atactcctta aggctacgcg aggctgcctc aacggcatcg gccatcatca
     7681 cgatagctgt ttccttggtt tgcggttctg gccctgggta tgagaactta ccctcgtcga
     7741 tatttgcatt ctcagcctgc aattccttgt tgtagaagta cttaactttg cttgtaccat
     7801 ggtgcatctt aataaagtca actatttgac tcggtaatcc tgccttagcg gcaattttca
     7861 ccccttcatg cacatgattt atgataatct ccgcgctttt aacattatca acattctcat
     7921 gaggattgaa tccaccctgc tgattctcaa tgaagaacat agggttagcc attttcccaa
     7981 tgtcgtggta tagggctcca gtcctaacca gtaaagggtt acctccaaca cgctgaacag
     8041 cgctttcggc taaattggcc acctgtaagg agtgctggaa ggttcccggg gcaacctcgg
     8101 ctagctttcg gagtaaatcg aggttggtat cggaaagctc aataagcgtg atatcggaca
     8161 aaaacccaaa aaacttctca ataatgtaaa ctaattggta cgaagcaaga agcaacaaag
     8221 cgtttatacc ataccataat gcaatgaacc agtttacccc tttgagtgag ccatcgccaa
     8281 caaggtatag gcttaatgtt aacgtcaagt aggagaagta aacaagtccc acagttttaa
     8341 atagttggcc gcgattgtac atttttgaca atccaattat tgccactatg ccagcaatga
     8401 agttggtgaa tacaaactca aagctgttgg ggactaaaaa tgctacaaga aaaatggtaa
     8461 taaggtgaac aaagagggca agccgcgaat cgaaaaatgt tttaacaaaa atgggtacaa
     8521 tagctagcgg aataacaaaa atactgaagg agttggtttt gattaccaga gccgaaactg
     8581 cagccgatgc cgttattaac attaatataa atagcagttt cctaatatcg ttcaatacct
     8641 cgtttcggaa attcaatagg aaaaggaaaa gaacaatgta gagcgatgca ataagtatca
     8701 tattcccaaa gagaatggca aataaatttt ttgatcccaa gcgtttatcg tactcttttt
     8761 tgagcgattc aagaatctgg taaatctctg agtttaccac ctcgcccttc accacaattt
     8821 tctcgccggc ttgtaccaac cccttggtaa ttgaaatatt tgatattagc tcattacgga
     8881 acttttgcgt tgcaacctca tcataaaaca ggtttggttg aatgtattcc ttaaggttaa
     8941 tcatttccca caaacctacc agcccaggac ttaagctttt agtgaagtcg tttgcatttg
     9001 aatttaagag cttaacagcc ttttgaaccg tataaacctc atcgaaccac ttgtcgtatg
     9061 ccacattatt gataacaaca gccaaaccct gcttgagcat gctgtttgag atggcgctct
     9121 catcctgatt gataattcct tttagataaa tctggctaat attatcggca acaaactgtt
     9181 ctagttccgg ttttactttc aggtactttg cccttgatgt ttttgaaaca ttctggctat
     9241 taataaattt atcaaatgcc aagtcgaaac tggaacgaaa cccctccagt tgctgagggt
     9301 gaatggtaga atcgtaccgg taaaaggact tgaactcctt tagcagcttg cttttctcag
     9361 caaaaagctc ttgctcagtt ttataaactg gaaaatcgaa aggggcataa agatcctcgt
     9421 gcatccaagg tcttcctttt tggaattcat agctaaatct accttcacat ggcattatta
     9481 taaccatgat gatgtaggat agtaggaaga gaacaaatgc cacgatgtgt tttatgctga
     9541 ttttttgggt tgctaccata accttttatt aataaaagta ataatcattt atttgtcaat
     9601 gagatgtatt gagggttaat atttatcgct gtcaaaccca actctttacc agcgtttagc
     9661 ataagggtat aggcttcaaa aaaatcgtta cggatttcgc catccagaat agcatcctta
     9721 atcctatttt taatgagtcc tacttcctta cagggcttca ggttaaaggt ttccataatc
     9781 atttcgccag taacgggtgg ctgaaagttc cgaatggaat ctttttcctc cacctcctta
     9841 agcttcttcc ttacaagtct aaaatttgcc aaatgtttcc gaacggtaat atcgttcttt
     9901 gaggtgatat cggcttcgca aagcatcatc aggtcatcaa tatcgtcgcc tgcatcaaat
     9961 agcagacggc gaacagccga atcggtaaca acctcctgcg ataaaacgat tggtctaaga
    10021 tgaagcagaa ccattttttg aacatatttc atcttatcgt taaggggcaa cttgagctta
    10081 cgaaatattt cagggatcat ttttgcgccc aaaaattcgt gtccatgaaa agtccaaccc
    10141 tgacccgcca caaagcgttt tgtttttggc ttgccaatgt cgtgaagcaa tgcagcccac
    10201 cgcaaccaaa ggttatcgct tttcaacgac aggttatcca acacctcaag cgtatggtaa
    10261 aagttatcct tgtgcgatac tccattacgc gcttccacac ccttcattgc cgccagttcc
    10321 ggaaaaatat gttgcaatag ccctgtttgt tcaagaagta accaccctac cgatggccgt
    10381 ggtgaagcca tcatcttatt tatttcgtcc acaacccgct caatggaaac aatagtaatt
    10441 cgcgagctca tttcacgaat agcatctagg gttttgagct caatatcaaa gtttagctgt
    10501 gttgcaaacc tgattgccct gagcatacgc aaagggtcgt cggaaaaggt ggtggcgggg
    10561 tcgagcggtg tgcgtattgt tttacttttt aaatcctgca aaccgttaaa cggatcaatt
    10621 agctcaccaa agtttcgttt gttcagccct attgcaaggg cattcatggt aaaatcgcgc
    10681 cttttctgat cgtcctcaag cgaaccgttt tcaactattg gctttcggga gtcgagtctg
    10741 tacgattcct ttcgggcgcc tacaaactca atatcccact cgttgtgctt tagctgtgct
    10801 gttccaaagt ttttgaaata ggttaccttc cgtgccctaa gcctttgagc caccttgcgt
    10861 gcaaaatcta ctccgctgcc tacaacaact atatcaatat ccttcgatgg tcggttcagt
    10921 aaaatatccc tcacaaagcc tccaataacg taagcatcaa ccccctcaat ctctgccaca
    10981 tccgatatta ctctaaatat gttgcttttt aggtattcct tcatattgtt atgttatgcc
    11041 tgacaaaatt acaacttatc aaacttttag cctaaatttt tgttttatag taaaaatgct
    11101 ttgtttaaaa catcgcaatg ataaaatgac atatatcagc ttaaaatatt caacaaaatt
    11161 atgccattaa atgttttggt attacttttg catcgaaata tgtaaataat ttgatttatt
    11221 aataaataaa ccagtaacac tatgattgag cacttaacag cagaaacatt caagcagaaa
    11281 gtatttgatt acgaaaagaa taacgagtgg aagtttgagg gcgacaagcc tgccatcatc
    11341 gatttctatg ccgattggtg tggtccctgc aaaatggtag cacctattct ggaagaactt
    11401 gccaaggaat atgagggcaa actcaatatt tacaaggtag ataccgagga agagcaggaa
    11461 cttgctgccg tttttggaat tcgaagcatt ccatcgttac tctttattcc acttaatgat
    11521 cagccacaaa tggctatggg ggcattacct aaggacacct ttaaaaaggc attcaaggat
    11581 gttttaggtg ttgagtaatt gaacctattt gtaataaagg aaaaggagcc tttggctcct
    11641 ttttttattc tggttgattt tgtgttagat tccagaaagc atggccttaa aaccttaacc
    11701 ctgaactttg aatttcgctt cgtgggactg gctctttacc cccgtagagc gaggcgggac
    11761 ttttatcctt taacctttat tcccgctaat gcgggacagg ctctttatcc tttaaccttt
    11821 aacccttatc ctttaatgac tatcaatccc catcagcatc aaaaacaaat gcttttgcac
    11881 ctttgaaatc tcacccatgt attcccttac agccttaacg cttccgggta atgtgtaaac
    11941 aaatgtagtt cccattaacc cagcaacgcc tctactaagc aaagcgtttg gcttttcggc
    12001 cccatacttt aagcggatgt gctccataat ccctggaatt tctttatcga gcaatggttt
    12061 tacaacatcg ggagtaatat cgcgcgggcc aattccagtt ccgccagttg ttaaaaccat
    12121 gtcgtattta gagtttcgtg cttcaatcag tctgttctct agcaaatctc tgtcgtcggg
    12181 aataatttca gtttccacct ctaactgcca ggatacggag ttaaagaaat cggaaacaag
    12241 tttagcaacc tcaggaccgc taaggtcctc atactcgcct ctacttgccc tgtcgcttaa
    12301 agtaattact ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttgtaaa
    12421 cttatcgata ctctcaacgc cgagcatgct cacctgacgg tgccaggcac cagcatgtgc
    12481 atcgccttta acaccaagcg agtttagctc aatacgatca actgcatgct tagttgttcc
    12541 ttttttttcg gagatattta ccgatacaac ctttacagta atgggattag tttgctccat
    12601 agctaaacaa tatccttttt agctttctca attagttcaa cgcgttcaat aaccatttgc
    12661 ttgtcaacag ccttgcacat gtcgtaaatg gtaaggagcg ccacggttgc tgctgttagt
    12721 gcttccatct ccacaccggt ttgcccaatg cacttggcgt aacttttaac ctcaacccca
    12781 ttttcgcaaa gggtagcttt aacatcaacc ttagtaagca tgagtgtatg gcaaagcgga
    12841 attagttcag gggttcgctt agcagcctgt atgcctgcaa tctccgcaat ggttaaaaca
    12901 tcaccctttt tcatttggtt ttcacggatc agctttaccg tttggggtag taaacgaata
    12961 aagccagtag cgctagcctc gcgtatttga ttgggtttat gaccaacatc aaccatattg
    13021 gccttgccat ttttatcggt gtgcgatagt tccatctcag cctcctatgt tataaaactc
    13081 accgctacgg tttgcaactc cgcaagctgg tttatttttt atagccattt caattgcttg
    13141 ctgtatgcct aactccctta cgttatatcc cttgtcgcta aaaaggcatg gtttaagaat
    13201 accatcggcg gttaagcgca acctgttaca ggtgctgcaa tttccaccct cgccaccctc
    13261 aacaacgcca aagacaccat catcgaggtt catctttggg ataaaacggg caataaagcc
    13321 attagagtta gcaaatgctt taacggcttg ggcatcgggt tcgttagagt tgttttctac
    13381 cacgcaattt aattttatgg ggttaaaccc cacctttttg gcttcctcaa gtccctccag
    13441 cacatcggat agcgtgccaa cccttgttat agccttatac ttttggggat ctaacgtatc
    13501 gagactaaca tttatgcgtt ttagtccggc atcgtaaagg ggctgagcaa attgttttaa
    13561 aagcgttccg ttggtagtca aacctaaatc ggtaataccc tcaactgttt taagcatcct
    13621 taccaagtcc acaattcctt tgcggactag cggttcgcca ccagtaatgc gaaccttatc
    13681 aaaaccatgc tgaaccgcaa tccgagctat ttctacaatt tcctcaaagg ttaagacctg
    13741 gttatggggt atttggacaa taccctcctc gggcatgcag taggtacacc gcaggttgca
    13801 cctatcggta accgatatcc ttaggtaatt aatgcgccta ttaaattggt cgaacatcca
    13861 aaagttctcc ttttttgagt tcttttactc caagcggaat agctgcaata cctgtagcct
    13921 ttgccaacga aaatatatgg gctgaaccat ggaactcaag aggttcaacc tcccctatat
    13981 cgttaacaaa aacagggaaa agccctaaac gttcagctcg ttttcgggaa taatcaattg
    14041 ctaagggcaa cttgtatgtc ctagcgtaag gctcattgca ttgcatcatc atcaggagtg
    14101 caggttttac cagcaactca aactgtacaa acgatgatac aggattaccg ggcaatccaa
    14161 atattaattt attgccagca actccaaatg tagttggttt tccgggttga actgctatgg
    14221 aatcgaatag gattttaaca cccaaacgct ccataattct tggaacataa tcaaaatcgc
    14281 ccattgaaac tccacctgta agcactacca catcgctttc agccaaagcc ctagcaatgg
    14341 ctttttcggt aacctcctcg gtatcgccta ctataccatg gtacattgga attgcacctg
    14401 ctgaaattac ctgtgcaatt agttgatgcc cgttgctatt cctaatcttg ctaccctgtg
    14461 gtatttcagt tggctcaaca agctcatcgc cggttggcaa aattgacact ctaggcctta
    14521 cagaaacgat cggggaatga catccgagtg cagccattat ggctatatgc tgaggcttaa
    14581 taagggttcc tttgcttaac ggcgactcac caattttgat gtcctcgcca agttcacaaa
    14641 tgttagattt tggtttactg cctttaaagc gaatagttcc atcggacaat atttcggtat
    14701 cctcaacggg tataacacaa tcagctccat tgggtatggg tgcgccggtc attatcttcg
    14761 agcatgtgcc ctgggctata gttctttggg gtatagtccc tgctgcaata acctcaatta
    14821 ctgtaagggg attatccaaa tctgcagccc tgcaagcgta accatcaaca gccgttttgc
    14881 ggaatggcgg catatttata tcggaaacca catcatgcct caacactcgg tttagacttt
    14941 cggataatga aacatgctct tgtccattca aagcggtaac gctttgttct acaatcctta
    15001 tcgccttatc taacgatatc ataacactga ttcattgttt tgcaaatatg tacaaaaaag
    15061 ttgttaccga aaagaatttt ttttcatatc gatttgtatt attaaaccaa agcaccatga
    15121 attaacaaat tcaacagggc taattttagg tgaatgaatc ggaatgaatc ggaataagca
    15181 gaatatgatg gagttagagg aaattagagg aagttagagg aatagtaaaa aagtgaacgg
    15241 ctaaaccaaa cgaacaagaa caaacaaata caaccgggct aattaaaatg tggatacaga
    15301 aacattttta gagaagatta gtcaatagaa acacaataaa acatttaaac aacttgtttc
    15361 tattaaatta tcgaggtaat cccgacttat caaaatcttt cattccgaga tctcggaatg
    15421 attagatttt gttagtcgat tcgtcaattg aaaagttcaa ttgacgaatt tggattcaat
    15481 gtcgtttagt taagggtatt tgtcatgttg cgcgcaagcg aaacatcaca agtcgtagtg
    15541 agtttcgatt tttagggata gacatcgccg tagcatctca ttgctctttt ctcttcaatc
    15601 ctttgaagag atccctcgcc tttgctcggg atgacaaaaa ccctatcttc ctttctgctc
    15661 ccttgtcatg ctgagctagc ccaagcatct ctttgtacct tcaactcttt taatgagatt
    15721 aataaaccaa caaagaatgg catagaaagg ggcataaaag cctgacccgg caatgggtcg
    15781 ggagtggaac gtggcggtgg gcttagtaag cgataccaaa tgagtcacgc cacaggcgtg
    15841 gcgaatagta tcgcgcggca gttccacccg cggggtaccc atcgggtcgg gcttttcagc
    15901 cttgattttc tttggttctt tcttgtatca agacaagaaa gaacgtgaaa atcactcttc
    15961 gcaatgtttt tcctttgcga tgcaaagcct caccattgct ctttggtata tgcattgcgt
    16021 gcatcgcaat acaagtggga gtaaacatag ggttacaaat gtttagcccc tacggggcta
    16081 gatgcgttgt tcgttattcg tgaatcgttg ttggtgaagc tattgtcatg ttgagcgtta
    16141 gcgaaacatc tcatgggctc gaaaagagat cattcgctgc gcgcaggatg acaggcatgt
    16201 atatttttgt taactaaacg acattgaatt tggattaaac atttactaaa aattagtagg
    16261 tttgggatat tttgaatctc aaacctaaac cataaaaact atgtcgcacg aacctgtaga
    16321 aaaaatcaaa ggccttccag aaaatgccta cactgagctc cgcgaaggcg aagagtatca
    16381 accagtaatg ccaccaagca aggcagtgcc tgaggttact gcttggagtg ttatttgggg
    16441 gctaatcatg gctattgttt tttcggcagc cgctgcctat ctcgggctaa aaattggtca
    16501 agtatttgag gcagcaattc ccattgcaat cattgctgta gggctatcaa cccttgctaa
    16561 acgcaaaggg gcactgggag agaatgtaat tatccaatcc ataggcgcaa gttctggggt
    16621 aattgttgct ggtgctattt ttacattacc agctctatac atcctgaacc tggaggcaga
    16681 attctatcag gtattcctgt catcgttgtt tgggggaatt cttggaattc tattcctcat
    16741 cccattccgt aaatattttg tggccgacat gcatggtaaa tttccttttc cggaggccac
    16801 agcaaccact gaggtacttg tatcgggcga gaaagggggg aaacaagctc gtttacttgt
    16861 ggttagcggg ctaattgggg gtatctacga cttcattatt gccacatttg gttggtggag
    16921 cgaggtggtt accacccgag tgatacctgc tggcgaggct cttgccgaga aagcaaagct
    16981 ggtatttaaa cttaatgttg gtgctgccgt tatgggtatt ggttatatta ttggcctcaa
    17041 gtacaccgca ataattgcag cagggtcgtt tgttgggtgg tttgttatca tacccataat
    17101 caactacttt gcgcccggtt taactgaggc agtaggcagt aacattacac taaccgttgg
    17161 gcagatgagc gccgagcaga ttttcagtaa ctacgttcgc ccaattggta ttggtggtat
    17221 tgccatggcg ggaataattg ggattattcg ctcaagcggc atcattcgta aagcatttgg
    17281 cctggccgtt agtgagttaa ccggaaagca ctcggctatc cccaaggagg aactccgcac
    17341 ccgaagggat cttcccatgt ccattattgc tggaggtatc ctattcacta ccattctgct
    17401 tttagtattc ttctactttg gagtagttca taatattacc catgctctta tagcgcttgg
    17461 tattgtaatg gttattgctt ttctgttcac caccgttgct gccaatgcca ttgccattgt
    17521 tggtaccaat cctgtatcgg gcatgacact catgactctg attatcacct cattggtaat
    17581 ggcatcggcc gggttatcgg gaacttcagg aatgattgct gcgctcatta ttggaggagt
    17641 ggtttgtaca gcactctcaa tggctggtgg ctttatcacc gatcttaaaa ttggttactg
    17701 gcttggctcc tcgccctata agcagcaaac ctggaagttc cttgggacat ttgtttcagc
    17761 agcaactgtt ggaggcgtaa taatcctgct taataaaaca tacggtttta ccggcaagga
    17821 cgctcttgtt gccccacaag ccaatgccat ggctgcagta attgaaccca tgatgagcgg
    17881 caagcctgca ccttgggcac tttacgctgc aggggcattc ctttcgctta tccttaccat
    17941 gattggagtt ccagctcttg cattttcgct gggtatgttt attcccctac aacttaacac
    18001 gccattactg gttggaggaa ttattgcaca cattgtttcc acccgttcaa aggacaattc
    18061 attgaacaga gccaggaaag aaaggggaac actaattgca tcgggcttta ttgcaggtgg
    18121 agccctaatg ggagtagtta gcgccttact ccgctttggc ggcattaact atgtaaaccc
    18181 agaatggttt gaatcgcact ccgccgaaat tctaggcttt gtgatgttcc tgattttatg
    18241 tggatatctg atttgggaaa gcctaagagc aaaggttgaa gaatagagaa tcgtgtctag
    18301 tttttggtgt ttagttttta gggactattg caaaatttta tgagattaat atgattgatt
    18361 ataaacaatt agatgtttgg atagctagca gacagcttgc aaattctatt tatacaattt
    18421 caaaatattt ccctaaagat gaaatttttg gattgacaca acaaataaga aaaagtgcca
    18481 ttttaattcc ttcaaatatc gcggaaggca ttggccgcaa tcatttaaag gagacaaccc
    18541 aattccttta cattgcaagg ggatcgttat ttgaattgga aacgcaaata tatatagctt
    18601 ttgatcaagg ttatattgat gctacagctc ttgaacaaat aacgaatgac attgagaaat
    18661 gcaagaaact gcttaatgga tttatcaact attatcttaa gaaaaaacat gaagaccaca
    18721 ctaatactaa aaactaaaca ctacaaacta caaactaaat aatccgccat gataaacaaa
    18781 caatctctta tcgaccgctt tatcaggtat gtaaagatag atacccaaag cgacgacact
    18841 gtaaccgaaa ggtttcccag cactgagaaa cagctggttt taagcaatct tttagtaaag
    18901 gaattaaagg agttgggggc tcaggaagtt gaaattgacc agtatggtta tgtgatggca
    18961 acaatacccg caaacacctc aaaaaaggtt ccggttttgg gtttccttgc ccatgttgat
    19021 acttcgcccg atatgccagg gaaagatgtt aatccacgct tcgttgaaaa ttacgatggg
    19081 tccgaaatta ttctgaacaa ggagttagga attgcacttt cgcctaagga attccctgag
    19141 cttaagaatt atgttgggca aaccctgatt gtaaccgatg gcactacgct acttggcgct
    19201 gatgataagg ccggattagc cgaaattatg acagcagttg agtacctcat caaaaacccc
    19261 agctttgaac atggtaaaat tcgtattggt tttacagttg atgaggaggt tggccgcgga
    19321 gttgaccact ttgacgtaaa gcgctttggt gccgattttg cttatacgct cgacggtggc
    19381 gctattggtg aactggaatt tgagaacttt aatgctgcaa gcgccaagat atccatccag
    19441 ggacgtaata tccatcctgg atatgcaaag gacaagatgt taaatgcaat acttatagcc
    19501 caggaattca acagcctgct acctgcacat gaacgccctg aacacactca gggttacgag
    19561 gggttctacc acttaatcaa gatggatgga tcagttgaaa aggcagcttt tcaatacatt
    19621 attcgcgacc acgatcgtaa aaagtttgaa gcccgaaagg cttatatgca gcgcatagca
    19681 gaatatttaa ataccaagta cggcgaaggt actgttactt tggagctaaa ggaccagtac
    19741 tacaacatgc gcgaaaagat tgagccggtt taccatgttg tttcaacagc catggaagcc
    19801 atgaaagccg ctggcgttaa tccaaaggtt aaacccatta gaggaggaac tgatggcgcc
    19861 cgcctttcct atatgggact cccctgcccc aacctatttg caggcggcga aaactttcat
    19921 ggaaagcatg aatacgtagc cgtagaaagt atggttaaag caaccgaggt aatgctcaaa
    19981 ataattgagc tttacgcatc aaagtaagaa aattcagtga taaaaaaacg agggagaaac
    20041 ctccctcgtt tttttatttt ttggtaatgg gctccaaaac ctcataaaat actctatacc
    20101 caccaggagc catgtagtta tagtttaggt agataatatc agttaccgga tcgtaatggg
    20161 agtaaaccaa gagattatta ggatcaccct c
//
LOCUS       DAOOWJ010000044        19702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2751_length_19702_cov_7.782664, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000044 DAOOWJ010000000
VERSION     DAOOWJ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 19702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19702
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2751_length_19702_cov_7.782664"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2426)
                     /locus_tag="PLW22_05995"
     CDS             complement(<1..2426)
                     /locus_tag="PLW22_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOU74290.1"
                     /translation="MDSTDTQYIFAIALHRHRELGLVAGAYLISPISDDTYKIEKNVI
                     VDSAVIHSDRITTDQSEIIRSIQEISEKSLLKLFGRDAKTFNELLQKVQSDENLNSYI
                     RNYIDRRICKAVEKAASANIPIYIRERNANNIYKEKQLTYHTDVASPIFRFELDELGM
                     KYSLKIQHQTSILTLLPGVFELLTVEPCLIRVANGIYKVEGIDGRKFVPYMTKQFVTI
                     PQQSVQKYMETFVLNAVRSNQVEAVGFEINEQKNRPKPILTLDERLSGGACLTLSFQY
                     GNKVISANTPAVNEVKLSFRNNSYTFTRLTRNPEKEEKIKQQLIKTWRLTKVGENEYF
                     PGKPENGEFDHYQVIEWLNTNYKLLQDEGFEIHQKFRQTNYYLSGFDLNLRLTESIDW
                     FDLYGMVRIGEFEIPFVYFRKHIVKNIREFTLPNGQVFILPDEWFAKYRTILMLGKEE
                     GESIKLSKASAAIIAGSSLETQSISSFAEKLKNINAKGVTLPHSLQAKLREYQTLGFA
                     WLKTLVENGLNGCLADDMGLGKTLQTITLLLSELEANDFKTGQEHCSLVVAPTSIIYN
                     WQKEFEKFAPQVKVGIYHGTQRDKSHKIFQQNHVVITSYGTIRNDIDLFKDFAFRYVV
                     LDESQTIKNPASKIYRCVLLLKTKKSLCLSGTPIENNLFDLWSQMNFLNRGMLGGLKV
                     FREEFAIPIERRNDAEKQKQLKKLIEPLIMRRTKEQVAKELPPLTEQIIYCEMTDEQG
                     KAYEAEKSKARRLILDSLEQSKPESSTINVLSALTRLRQLANHPAMLDEYSNLSSGKF
                     EEVTQMIESV"
     gene            2609..4459
                     /locus_tag="PLW22_06000"
     CDS             2609..4459
                     /locus_tag="PLW22_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU74291.1"
                     /translation="MIKRILALLTLVSLSYILFSQEVEVKISKDKVRVDGKTYFVHIV
                     QQGETLYSISKAYGVSQSDIAINNPDIYAGLKVGQALKIPAKVKETQTDEDYIYHIVK
                     RKETLFGISRMYNVSMDDILRLNPEAREGLKLSQTLRIPKKQISDIEQQPAVDSVEFI
                     LHEVQPREGLFAISRKYGVSPKEIEYYNYDLIKDGLKLGTTLRIPKPKSVPVPEAVEA
                     KVDTDGQEKASPCSNSYTYDGHPFNVSLLLPFTQSGSQTQAIVDDVDPKDVQDPTGQL
                     TQITQVSLEFYEGFLLALDTLKKLGISVNLNVRDTKRSPQEVAKILNSADFARSELVV
                     GPYMFDEVKPVAQFAAKNNINFVSPIYSIKEGLADKQNIISVSQTLQNQLEIFVKNLK
                     VDPSKNYLVIYDSTIWYSPSLKHFDSLLTLSFTNAAIPISRYNHKTAAYKSVDVQERL
                     LKMLKRDAVNVVIVPSDDEPFVAEILGSLYAVKSYYNLNTEVYGPSRWRKLKNLPPDY
                     LFALNTHVFSPFFVDYSQSDVKTFVEKYRVQFRSEPTQFSFLGYDVAFYFINALKEFG
                     PGFTPCLPNFRLRTLQTNFKLNTSKEGYTLNSNQFIVRYAPDFTITCSCY"
     gene            4483..5385
                     /locus_tag="PLW22_06005"
     CDS             4483..5385
                     /locus_tag="PLW22_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU74292.1"
                     /translation="MNYRFNSGNTRLVNFEGWLSVLINTLLFVLKYWAGVVTGSLALI
                     ADAWHTLSDSISSVVLLLGIKFSQKPADKDHPFGHGRSELVVSLLIGALLAFVAFYFA
                     MEGIAKIRDRVSTSYGTLAIVATVVSIVVKEGLAQVAFIIGKRTGSISVTADGWHHRS
                     DALSSVIVLVGIFVGKYFWWIDGVLSIIVALLILYSGIKVITDSAVVILGEVPSDDLV
                     QKVMEIVSRQGYDRMEPHHFHIHNYVMHQELTFHVRLPNQMTIEQAHRIISLIEDAIH
                     LELGIDATIHIEPQTTDKHCKHID"
     gene            5535..7151
                     /gene="pckA"
                     /locus_tag="PLW22_06010"
     CDS             5535..7151
                     /gene="pckA"
                     /locus_tag="PLW22_06010"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOU74293.1"
                     /translation="MEMIDLSQYGINSLRELYYNPSYDLLYEHETDPNLEGYERGFVT
                     KLGAVNVDTGVFTGRSPKDKYVVREPNTENDVWWAESGKKGSDNKPISQEVWEKLKEV
                     SVKQLNGKKLYVVDAYAGANEDTRLSVRFIMEVAWQAHFVKNMFIRPSDEELKNFTPD
                     FVVLVASKTTNPYWKEQNLNSEVYVAFHLTERMALIGGTWYGGEMKKGIFSVMNFYLP
                     LKGIASMHCSANVGKAGDVAIFFGLSGTGKTTLSADPNRALIGDDEHGWDDSGVFNFE
                     GGCYAKCIDLDPQKEPDIFAAIRRDALLENLDVDAEGNIDFHSKRKTENTRVSYPIYH
                     IKNIVKPISRAGHAKNVIFLTADAFGVLPPVSKLTPNQTQYHFLSGFTAKLAGTERGV
                     LEPQPTFSSCFGQAFLMLHPTVYARELVKKMQQHNSKAYLVNTGWIGGAYGVGRRIDL
                     PSTRAIINAILDGSLDNAEFDVLPIFNLQIPKALPGVDSKLLNPRNHWSNPEDWDAAA
                     RNLAQKFIENFKTFTDNEWGQRLVAAGPML"
     gene            complement(7235..8182)
                     /locus_tag="PLW22_06015"
     CDS             complement(7235..8182)
                     /locus_tag="PLW22_06015"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOU74294.1"
                     /translation="MKAITRTDFKFPGQVSVYHGKVRDVYNINNQFLVMVVTDRISAF
                     DVILPKGIPYKGQVLNQIANKFLDATADIVPNWKIASPDPMVTVGRFCEPFKVEMVVR
                     GYLTGHAWREYRSGKRSLCGVPLPEGMKEHQKFPKPIITPTTKAEEGHDEDISREEII
                     AKGLVSEADYNLLEKYTLALFERGTQIAEKMGLILVDTKYEFGKKDGKIYLIDEIHTP
                     DSSRYFYLDGYKERFEKGEEQRQLSKEFVREWLMANGFQGKEGQTVPEMTPEFIEQVS
                     NRYIELYEKITGDRFQKAHSDEDILNRIEKNVKAFLQNM"
     gene            complement(8194..9195)
                     /locus_tag="PLW22_06020"
     CDS             complement(8194..9195)
                     /locus_tag="PLW22_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOU74295.1"
                     /translation="MAAGERTIIIEGIDPLVLYGVNNTRFDLITKHYPKLKIIGRGNT
                     IKAVGDEQLIEDLDQKVHAMIDFHIKYGYLSETNIKDILQNNPESIEKAEEDITDVLV
                     YGNHGKIVKARTVNQRKLVEMYESNDLIFAIGPAGSGKTYTAIALAVRALRNKEVKRI
                     ILTRPAVEAGERLGFLPGDMKEKLDPYLQPLYDALHDMIPPKKLQEFMEDGTVQIAPL
                     AYMRGRTLDNAFVILDEAQNTTLSQLKMFLTRMGSNAKFIVTGDITQIDLPNKDDSGL
                     VKAIKLLEGIKDIAIIYFDTRDIIRHKLVKYIVNAFEEAKGKTDIQTDAKSDDNQTK"
     gene            9369..10172
                     /locus_tag="PLW22_06025"
     CDS             9369..10172
                     /locus_tag="PLW22_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOU74296.1"
                     /translation="MAATNTRFVTLTTDWGIQDYYLGMLCGKLYSISPNLKVVELSHK
                     VPTYNYMHAAFLIKHSFSHFPKGSIHICMVNSDTSDNMRMLFFEYQGHYFITPDNGTI
                     SLIADVLPEVVRAIPVDDTRSFGSIDSVTLALEALLSENGQTDFAIEASGIKRFTPIL
                     PVPENDGITGSVIYIDSYFNAITNITRDDFEAIRKSRKYSIYVHRFSHGISKLSNSYK
                     DVEKGDLLALFNSLGLLEIAVREGFAAQMYNLSVGSNIMVRFYDDKILR"
     gene            10172..10966
                     /gene="mazG"
                     /locus_tag="PLW22_06030"
     CDS             10172..10966
                     /gene="mazG"
                     /locus_tag="PLW22_06030"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOU74297.1"
                     /translation="MNDSVRKQRASEAFQRLIGIMDDLRAKCPWDREQTFESIRPLTI
                     EETYELSEGILEKDYNLIRKELGDLLLHIVFYSKIAEEDNRFDIVDVINSLCDKLIYR
                     HPHIYGNVKVNDASEVVENWEQLKIKEKDGNKTVLSGVPTALPALIKANRIQEKVRAV
                     GFDWDERSQVWDKVQEELNELRHEVEVTKSTDDIEKEFGDLLFSVVNAARLYGVDPET
                     ALERTNRKFMKRFTYLEQQTIQMGRSLKDMTLDEMNEIWEEAKRFD"
     gene            10979..12061
                     /locus_tag="PLW22_06035"
     CDS             10979..12061
                     /locus_tag="PLW22_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU74298.1"
                     /translation="MFLYLVRKLFYTFLVIFGVASLVFILFNLIPGDPARMVLGQRTD
                     SLSVKAARTDLGLDLPVWKQYLKYLNDISPLSVHNPSNSKSSIYLDTLIYAKPLVLGK
                     MASNRVLVAKQPYLRRSYQSRQPVSTVVMSTLPNTFYLALVAIVFATIVGIAMGITAA
                     IYKDSVWDRLLLVNSALGMALPSFFAAILIGWLFAIVLGKYTGLNLTGNLWEVDDLGE
                     GIHLKWQNIILPALTLGLRPLGIITQLMRSSLLETLSQDYIRTAKAKGLPNRKVIFKH
                     ALRNSLNPVVTSISGWFASMMAGVVFVEYIFSWKGLGYVMVNALNSYDVPLVIGCVLT
                     VAVIFSIVNLLVDILYGILDPRVRIG"
     gene            12143..12898
                     /gene="tpiA"
                     /locus_tag="PLW22_06040"
     CDS             12143..12898
                     /gene="tpiA"
                     /locus_tag="PLW22_06040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU74299.1"
                     /translation="MRRKIVAGNWKMNTLPSEGLALAKDVIAKSAEVSSGVELVVIPP
                     FTHLNEVTKLASGTRVAVGAQNCARWQSGAYTGEVSAAMLGAMGVEYVIIGHSERREY
                     FGETNSQLYDKIQQALSSNLMPIFCCGEKLDEREANQHFQVVKQQVEESLFALDEQSI
                     KQVVIAYEPVWAIGTGRTATAEQAQEMHAYIRKLLAGKYGSQIADSISILYGGSCKPS
                     NAAEIFAKPDVDGGLIGGASLVAADFIAIAKSF"
     gene            12900..13736
                     /gene="prmA"
                     /locus_tag="PLW22_06045"
     CDS             12900..13736
                     /gene="prmA"
                     /locus_tag="PLW22_06045"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOU74300.1"
                     /translation="MGYTELKIRIKPYTAETAEILIAQLAELGFESFMENEPELLAYI
                     NTATLKKLPADFIGLIELPEGVRIDYELNSIADKNWNEVWESNFEPIIVDNRCLVKAS
                     FHTDTPKTDYEIVIDPKMSFGTGHHQTTHLMIEALLNHQVEGLTVLDMGCGTGVLAIL
                     AEMRGAQNIVAIDNDEWAYRNTLENIDNNSCKRITAYLGDAELLKGRSFDLILANINL
                     NILLADMAAYVNSLKSGGVIFMSGILRDDVETLRNAAERLGLSFSEVNYRDNWAMVAF
                     RK"
     gene            13761..18347
                     /locus_tag="PLW22_06050"
     CDS             13761..18347
                     /locus_tag="PLW22_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74301.1"
                     /translation="MAKPYVYRRILIIALLLTAFSLLYAVFISQDKKFSGQVPLFPAE
                     LNSLVSDKVKEQEEFFLKGFPAFWLSDTIEISKKEAIIGTANQILEVLPDDKSYILSY
                     VKLIADFYRNQYAKNQFETWFSYLNMALADGFIKSDNLRTLIRFSGSLIDSSFISIST
                     SHTWKVLPANSYTFTLKNNDLLVSFQNVNLICRNNRTDSIFIRGTSGNLDIIKQSWEG
                     KNGKVTWIRSKFDESKIFVALNRYRIELRQSEYTADSVLLNYPEYFKKPILGRIVDKV
                     TPIYQSGIVDYPEFNPYQKWFEVKNIFKDVDYSGNFSITGSKLVGIGPDGGLAKVTIH
                     RKGKPFMVAEGRMVLIEQSHLSSDKAKVVFYIDKDSIYHNGLSFAYHNPTRTVMVNPT
                     DKLTTQSPFYSSYHKINLWSNQLTWSIEKDEITFGSSLGASISKAEFESENFFNQDMF
                     DSMMDASEFHPVLTVWNYTRRIKSNTFLASDLAVHVRRAPEDVKIAMMRLAKLGYVLY
                     NFETDEVTITDKLKYNVMARFGRTDFDVIRFQSTTPGTQPNARLDLNNLNLAIEGVNY
                     ISVSDSQNVFISPYKKSITFQKNRNFEFGGSIRAGLFTFYGKAFRFDYSQFKVELNKV
                     DSLIIDYQTDYRDNYGRRILQGVANALHIISGDILIDKPYNKSGREYNPQYPIFNCTS
                     KSYVYYDASYIYGGIYKRDSFYFEIQPFVYQSMDNFEKADMNFKGILYSGNILAPIEE
                     TLRIRPDNSLGFITTTPSEGMAVYKGKGRVFNKIDLSNQGLFVNGSIDYITSTTQSDK
                     MLLFPDSMVTVSTGFSIAKRETGIEFPNVKGNKHSIKWLPKADKMFISKGEKPFVMYD
                     SLATFSGNLLLQPLGLTGAGLLNVPNGKLSSSLFEYSSNALAANESTLTILKTNTDSV
                     ALETSKVKATINFANMLGEFSRVDKSIYASLDALRYSTYHDRFKWDLKGYKVYFETLM
                     AQEDVLAGKFRVSNMIDRDSIPRGNLFFSTKPAEDSLYFFAPKAVYNTDRIHLVADSV
                     KYIVTADATVFPKNKRVEVDVPNRMYKFSNAKVRANNTTAYHVLHDAEIKINGRKSYG
                     GSAYYDYVDERDSIQTIYFPKVYSDNNMNTYAEGTLTEPDSFRLSPNFAFIGGVSLSA
                     PEKFLFFKGGAKPIYNCPGTIGQWVRFESQINPKRVLIPIGPDPRNMNLHRLISGTAI
                     AEDSLKLYGGLLRSRKFWEDSSLVTATGFMEFNNKNRRFTIAPLYKLDNPDSSGNSVS
                     LQKDYCWAFGEGNVRLPLKLGHVTSQVYGSVIHKLDKNEATIDAIIRLNFHFNQKALE
                     AMAAQLSGAITLDKVDLSRKLYRKMLYDWLTPKELPEALNQLSLFGSFTQMPKSLEST
                     ITLSDVKLRWDPQNKSFVSVGKIGVGTIGNIQVNRFVNGFIEYYKGRFNDRLILYIHI
                     GDNEYYSFYYANGVMFVSATNPEFYNPIASQKSSERRVKPSKGMMGYRYMIGSKRELN
                     RAQLRYKELAFGIKSDIPVDSDDDKPNKEGKEDD"
     gene            complement(18344..18841)
                     /locus_tag="PLW22_06055"
     CDS             complement(18344..18841)
                     /locus_tag="PLW22_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU74302.1"
                     /translation="MKAIGILSDTHGFWDKGLTDFFETCDEIWHAGDIGSLELADNMV
                     KLKPLRAVHGNIDDYKTRLVYPEKQLFTTENVNILITHIAGTPGHYDQKVLEILKGNK
                     VNILVCGHSHILRVKYDKQWDMLYINPGAAGNSGFHQVKTAIRLKIDGSNISDLEVWE
                     KKRGF"
     gene            19110..>19702
                     /locus_tag="PLW22_06060"
     CDS             19110..>19702
                     /locus_tag="PLW22_06060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOU74303.1"
                     /translation="MQIFYAPQIEGDEYRLPEDESKHCIRVLRMQQGDELYLTDGCGN
                     LHTARIADENPKRCLVQIVSTQHEYGKRPFNLHIAIAPTKNIERFEWFLEKATEIGVD
                     TITPLLCERSERKDIKVERLQKIVISAMKQSVKAYLPIINSIQRLRDFVKHTHQADLK
                     AIAHCNSWDLTPFQQVIHRGRSVLVLIGPEGDFTPAEV"
BASE COUNT         5417 a         4068 c         4304 g         5913 t
ORIGIN      
        1 ataacacttt caatcatttg cgtaacttcc tcgaatttac ccgacgacag gtttgagtat
       61 tcatcgagca tggccgggtg gttggccagc tggcgcaaac gggtaagcgc tgaaagaaca
      121 ttgatggttg acgattcagg tttactctgt tccagggagt caagtatcaa cctgcgggct
      181 tttgatttct ccgcctcata ggctttgcct tgctcgtcgg tcatctcaca gtagatgatt
      241 tgctcggtta ggggtggtaa ctccttggca acctgctcct tggtgcggcg cattatcagg
      301 ggttcaatga gcttttttag ctgtttctgc ttttcggcgt cattcctgcg ctcaatggga
      361 atggcaaatt cctcacggaa taccttaagg ccgccgagca tcccgcggtt taggaagttc
      421 atttgcgacc ataggtcaaa taggttattc tcaatgggtg ttccgctaag gcacaagctc
      481 ttctttgtct taagcaatag aacgcacctg tatattttag atgctgggtt tttaatggtt
      541 tggctctcgt ccaacaccac gtagcggaat gcaaagtcct tgaaaaggtc aatgtcgttc
      601 cgtatggtgc catacgatgt aatcacaaca tggttttgct gaaatatttt gtggctcttg
      661 tcgcgttggg ttccatggta aatcccaact ttaacctgtg gcgcaaactt ttcgaactcc
      721 ttttgccagt tgtaaattat tgaagtggga gcaacaacca gcgaacagtg ttcctgtccg
      781 gttttaaaat cgttagcctc aagttccgag agtaaaagtg taatggtttg tagtgttttt
      841 ccaaggccca tgtcgtcggc aagacagcca ttcaagccgt tttcaaccaa tgtttttagc
      901 caggcaaaac caagggtttg gtactcgcgt agttttgcct gaaggctatg gggtagggtt
      961 acccctttgg cgttaatatt ttttagtttt tcagcgaaac ttgaaatact ctgggtttct
     1021 aggctactgc ctgcaataat tgctgccgat gctttggaca gcttaatgct ttcgccttcc
     1081 tccttgccaa gcataaggat ggttctgtac ttggcaaacc actcatcggg caaaatgaaa
     1141 acctgaccgt tggggagggt gaactcgcga atgttcttaa ctatatgctt gcggaagtaa
     1201 acaaagggga tttcaaattc gccaatgcga accatgccgt aaaggtcgaa ccaatcgata
     1261 ctttcggtaa gcctcaaatt aaggtcaaag ccacttaggt agtagtttgt ttgcctaaac
     1321 ttttgatgaa tctcaaaacc ttcgtcctgt aaaagcttat agttggtgtt cagccattct
     1381 atcacctggt agtggtcaaa ctcgccattt tcgggtttgc cgggaaagta ctcgttttct
     1441 cccacctttg ttaggcgcca ggttttaata agctgttgtt tgattttttc ttccttctcg
     1501 gggttgcggg taagccgggt aaatgtgtat gagttattac ggaacgatag ctttacttca
     1561 ttaacagcag gcgtgtttgc cgaaataacc ttattcccat actgaaagct aagggttagg
     1621 caggctccgc ctgataggcg ctcatcgagc gtgagtatgg gtttaggcct gtttttttgt
     1681 tcgttgattt caaatcccac agcttccacc tggttgcttc taacagcatt cagcacaaag
     1741 gtttccatat acttttgtac cgattgttgg ggtattgtaa caaattgctt ggtcatgtag
     1801 ggtacaaact ttcggccatc aataccttca accttatata tgccattggc aacccgaatt
     1861 aagcatggtt caaccgtaag taattcaaac acaccgggca ataaagttaa aatggaagtt
     1921 tgatgttgaa tcttcaggga atacttcatg ccaagctcat caagctcgaa cctgaatatg
     1981 ggcgacgcaa catcggtatg gtatgttaat tgcttttcct tgtaaatgtt attggcgttc
     2041 cgctctctaa tatatatcgg gatattggcc gatgcggctt tttcaacggc cttacatatt
     2101 cttcggtcaa tgtagttcct gatgtagctg tttaggtttt cgtcgctttg aactttttgt
     2161 aaaagttcgt tgaaagtttt cgcatcgcgt ccaaatagct tcaaaaggct tttttctgat
     2221 atttcctgaa tgctgcgtat tatttccgat tggtcggttg taatcctgtc ggaatgaata
     2281 actgctgaat cgacaataac attcttttca attttatagg tatcatcgct gattggactg
     2341 ataagataag ctcctgccac caaacccaat tccctgtgcc tatgcaaagc aatcgcaaag
     2401 atgtattgtg tatcagttga atccatttag gctattttac tgcaatttta acaaaaatat
     2461 gcgtagctta atgtttttta tgtatcaatt tttactttct tcgttttaaa acagcattag
     2521 gcatagggtt tgaattgatt gatttgtgta tttttgtact ttgtattcgg gcccatttcg
     2581 gaataacatt ttcataaaac tacttgcaat gattaaaaga atacttgcac tcctaactct
     2641 ggtatcgctt tcctacattt tgttttcgca ggaagtagag gtaaaaatat caaaggataa
     2701 ggtacgtgtc gatgggaaaa cgtactttgt tcatatagtc cagcagggtg aaacgctata
     2761 ctctatcagt aaggcttacg gcgtaagcca gtccgacatt gcgataaaca accccgatat
     2821 atacgcaggg ttaaaggtgg gacaggctct aaaaataccg gctaaagtga aggaaaccca
     2881 aactgacgag gattatatct accacattgt taaacgcaag gaaacccttt ttggcatttc
     2941 aaggatgtac aatgtgagta tggatgatat actacggttg aaccctgaag caagggaagg
     3001 gttaaagcta agtcaaaccc ttcgcattcc caaaaaacag ataagcgata ttgagcagca
     3061 acccgccgtt gattcagtag aatttatttt gcacgaggtg caaccccgcg aaggcttatt
     3121 cgcaatttcc cggaaatacg gtgtttcacc caaggagatt gagtattata actacgattt
     3181 aattaaggat ggtttaaaac ttggaacaac ccttcggata ccaaaaccca aatcggtacc
     3241 tgttcctgag gctgttgaag ccaaggttga taccgatggg caggagaaag catcgccctg
     3301 cagtaatagc tacacctacg atggtcatcc gtttaatgtt tctctactac ttccctttac
     3361 tcaatccggc agccagactc aggccatagt tgatgatgtt gacccaaaag atgttcagga
     3421 ccccacaggg caattaactc aaataacaca ggtttcgctt gaattctacg aaggtttcct
     3481 gttagcccta gatactctta aaaagttggg catctcggta aacctaaacg tgcgcgacac
     3541 aaagcgctca ccacaggaag ttgctaaaat tctgaatagc gctgattttg caagatctga
     3601 attagtggtt gggccttata tgttcgatga ggttaagcct gtagctcaat ttgccgccaa
     3661 aaataatatc aactttgtat cgcccattta cagcattaaa gaggggttgg ctgataagca
     3721 gaatattatt tcggttagcc aaacacttca aaaccagttg gaaatatttg tgaagaactt
     3781 gaaggttgat ccaagtaaga actacctggt gatttatgac tcaaccatct ggtactcgcc
     3841 cagtttgaag catttcgatt cgcttctaac cctaagcttc acaaacgcag ctattcctat
     3901 ttctcggtac aaccataaaa ctgctgctta caaatcggtt gatgttcagg aaaggttgct
     3961 gaagatgcta aaacgcgatg ccgtaaacgt tgtgattgtg ccttccgacg atgaaccgtt
     4021 tgtagccgaa atacttggca gtttatatgc tgtaaaatcg tactataacc tcaacactga
     4081 ggtgtatggc ccatcgcgtt ggcgtaaact taaaaacctt ccccccgatt acctttttgc
     4141 attgaatact catgtattct caccattttt tgtcgactac tctcaaagtg atgtaaaaac
     4201 ttttgtagag aaatacaggg tacaattccg gtcggagcca acacagttct cgtttttagg
     4261 ttacgatgtg gccttttact ttattaatgc actcaaggag tttggtcctg gttttactcc
     4321 ctgtctgcca aatttcaggt taagaacgct tcaaactaat ttcaagctca atacgagtaa
     4381 agaggggtat acacttaatt caaatcagtt tatagtaagg tatgcccccg attttacaat
     4441 cacatgcagc tgctattaat ggatttaaat taaaatttta tcatgaatta tcggtttaat
     4501 tccggaaata cccgcctggt taattttgag ggttggctct ctgtcttaat caacactttg
     4561 ctttttgtac taaagtactg ggcaggggtg gttacaggat cgttagcgct tattgccgat
     4621 gcctggcata ccctttccga ttcaatcagc tcagttgttt tgctgcttgg cataaaattt
     4681 tcgcaaaagc ctgccgataa ggatcatcct tttggacatg gacgttcgga gctagtggtt
     4741 tctttgctta ttggtgcact gcttgccttt gtggcttttt actttgccat ggaaggtatt
     4801 gcaaaaatca gggatagggt gagtactagc tatgggactc tggcaattgt agcaactgtg
     4861 gtatcaatag ttgtaaagga aggacttgct caggtagctt tcatcatagg taaacgcaca
     4921 ggctcaattt cagttacagc cgatggttgg caccaccgca gtgatgccct ttcatcggtc
     4981 attgtgcttg tgggtatttt tgtaggcaag tacttttggt ggatcgacgg ggttttgagt
     5041 atcattgttg ctctgctcat tctttactca ggtattaagg tgattaccga tagtgcagtg
     5101 gtaattcttg gcgaggtacc atccgatgat cttgttcaaa aagtaatgga gattgtctca
     5161 aggcagggat atgatagaat ggagccccat cacttccata tccataacta tgtaatgcat
     5221 caagaactta cctttcatgt taggctgccc aatcaaatga ccatagaaca ggcccataga
     5281 attatttcat taattgaaga tgccatacac ttggagcttg gcattgatgc cactatacat
     5341 atagaacccc aaactaccga taagcattgc aagcatatcg attaatcttt ttggggattt
     5401 aatgggtgta cgattatcta cattattccc cttaaaagta acaatatgat acaaaacatg
     5461 ctgaaaaaat cgtttgcata aactaaaata tttacatttg cttataattc tcaattgttt
     5521 attcttaaaa aattgtggaa atgattgact tatcgcagta cggtattaat tcccttaggg
     5581 agttatacta taatccatcg tacgatttac tttatgagca cgaaaccgac ccaaatcttg
     5641 agggttatga gcgaggtttt gtaaccaaac ttggagcagt aaatgttgac actggtgtat
     5701 ttacaggccg ttcacccaag gataagtatg tggttcgaga gccaaatacc gaaaatgacg
     5761 tttggtgggc cgagtctggc aaaaagggtt ccgataataa gcccatatcg caagaggtat
     5821 gggaaaagct caaggaggta agcgttaagc agcttaatgg caaaaagcta tacgtggttg
     5881 atgcttacgc tggggctaat gaggataccc gattgagcgt tcgctttatc atggaggttg
     5941 cctggcaggc acattttgtt aaaaatatgt ttattcggcc ttccgatgag gaactcaaaa
     6001 actttacccc cgactttgtt gtgcttgttg catccaaaac caccaatcca tactggaagg
     6061 aacaaaacct gaacagcgag gtatatgtgg cttttcatct tactgagcga atggccctga
     6121 ttggcggcac atggtatggt ggagagatga aaaagggtat cttttccgta atgaactttt
     6181 acctacccct taagggtatt gcatcgatgc attgcagcgc caatgttggc aaggctggcg
     6241 atgtggcaat tttctttggg ctgtcaggca caggcaaaac taccctttcg gctgatccta
     6301 atcgagccct tataggcgac gatgagcatg gctgggacga ttcgggtgta tttaactttg
     6361 agggaggttg ttatgccaag tgcatcgacc ttgacccaca aaaagaaccc gatatctttg
     6421 cagccatccg gcgcgatgct cttcttgaga acctcgatgt ggatgccgaa ggcaatatcg
     6481 atttccattc aaaacgtaaa actgagaata cgcgagtatc atacccaatt taccatatta
     6541 agaatattgt taaacccatt tcgcgtgctg gacatgcaaa gaatgtaata ttcctaaccg
     6601 ccgatgcgtt tggtgtttta cccccggttt caaagctaac tcctaaccaa acccagtacc
     6661 actttttaag tggctttact gcaaagctgg caggaactga gaggggagtg cttgagccac
     6721 agccaacctt tagtagctgt tttgggcagg cctttttgat gttacaccct acggtttatg
     6781 ctcgggagct tgttaaaaaa atgcagcaac acaactctaa ggcttacctt gtaaacaccg
     6841 gctggattgg tggggcttat ggtgttgggc gtagaattga tttgccctca acccgtgcca
     6901 ttatcaacgc aattctggat ggtagcctcg ataacgctga gttcgatgtt cttccaattt
     6961 ttaaccttca aatacctaag gcattgcctg gtgtcgattc caagctttta aacccacgta
     7021 accactggtc gaatcctgag gattgggatg cagctgcccg gaatcttgcc caaaaattta
     7081 tcgaaaactt taagaccttt actgataacg agtgggggca acgattggtt gctgctgggc
     7141 ctatgctgta actgttctcc gaaaaaatca aaacctaaaa ttactataca agaatggcaa
     7201 ccattttggt agccattctt attcttgttc aatactacat gttttgtaag aaagccttaa
     7261 cgtttttctc aatcctattc agaatatcct cgtcgctgtg agccttttgg aatctgtcgc
     7321 cagtaatttt ctcgtacagc tcaatgtagc ggtttgatac ctgctcaata aattcagggg
     7381 tcatttcggg tacggtttgc ccctccttac cttggaaacc attggccatg agccattcac
     7441 gaacaaattc cttcgagagt tgacgttgct cctcgccctt ctcaaaccgt tccttgtagc
     7501 catcaaggta aaagtaccgc gatgagtcag gcgtatggat ttcatcaatc aggtatattt
     7561 tcccatcctt tttgccaaac tcatacttgg tgtcaaccaa aataagaccc attttctcag
     7621 caatctgggt tccccgttcg aacaatgcca gggtatattt ttctagaagg ttgtaatcgg
     7681 cttcgctcac taaacctttc gcaattatct cttcgcgtga aatgtcctca tcgtgcccct
     7741 cttcggcttt ggtggtggga gtgataatag gtttcgggaa tttctgatgc tccttcatgc
     7801 cttcaggtag gggaacaccg caaagtgagc gcttgccgga tctgtactca cgccaagcat
     7861 gacctgtcag gtagccgcgc accaccattt caaccttgaa gggttcgcag aagcgtccaa
     7921 ctgttaccat tggatcgggc gaggcaattt tccagttggg aacaatatcg gcagttgcgt
     7981 caagaaactt gttggcaatc tggttaagaa cttgaccctt gtaaggtatc cctttgggaa
     8041 ggattacatc aaaggccgat atcctgtcag ttactaccat aacaagaaac tggttgttga
     8101 tgttgtacac atcgcgtact ttaccatggt acactgaaac ctgtcctgga aacttgaaat
     8161 cggtgcgggt gattgctttc atcttttttg tttttatttg gtttgattat catcactttt
     8221 cgcatcggtt tgaatatcgg ttttgccctt ggcctcctca aatgcgttta ctatgtattt
     8281 taccagctta tggcgaataa tgtcgcgggt atcaaagtat attattgcta tatcttttat
     8341 tccctccagg agtttgattg ctttaaccaa gcctgagtca tccttgtttg gtaggtcaat
     8401 ttgtgtgata tcgcctgtta caatgaactt tgcgttgctt cccatgcggg taagaaacat
     8461 tttcagctgg ctaagagtgg tgttttgggc ttcgtcaagt attacaaagg cattgtcgag
     8521 cgtacgacct cgcatgtatg caagcggggc aatctgaacc gtgccatctt ccatgaactc
     8581 ctggagtttc tttgggggaa tcatatcgtg tagggcatcg taaaggggtt gcaggtatgg
     8641 gtcaagcttt tctttcatat cgccaggtaa aaagcccaag cgctccccgg cttcaacggc
     8701 tgggcgggtc aatattatgc gttttacctc cttgttgcgt agcgccctta ctgcaagggc
     8761 aatggctgta tatgttttcc ccgatccagc cggaccaatg gcaaaaatca ggtcgttact
     8821 ttcatacatc tccacaagct tgcgctgatt taccgttcga gccttaacaa ttttaccatg
     8881 gttgccgtaa accagcacat cggttatgtc ctcttccgcc ttttcaatgg attcagggtt
     8941 gttttgcagg atatctttga tattggtttc acttaagtac ccatacttga tgtgaaagtc
     9001 aatcatggca tgaactttct gatccaagtc ctctattagc tgctcatcgc caacagcctt
     9061 aatagtgttc cctcttccga tgatttttag ctttgggtag tgcttggtta ttaagtcgaa
     9121 gcgggtattg ttaaccccat ataacaccaa ggggtcaatc ccttcaataa tgatagttct
     9181 ctcgcctgct gccatcaata cctgttgggt tatgtatgca aagataatct aaaaccagcc
     9241 aacttttgtg tttatagtcg caagatatca aaaaatacta acagctaaat ggttagtaag
     9301 tttagcaggc taaatgcaga aattattaat tttctagttt acttttgtat tgatttttgg
     9361 tttcttaaat ggcagcaacc aatacccgtt ttgtaactct taccaccgat tggggcatcc
     9421 aggattacta ccttgggatg ctctgcggaa agctttattc catttcaccc aacttgaagg
     9481 tggtggagtt aagccataaa gtacctactt ataattacat gcatgcggca tttctgataa
     9541 agcattcctt tagccatttc cctaaagggt caattcatat ttgcatggtg aatagcgata
     9601 cctctgacaa catgcgcatg cttttttttg aataccaggg ccattacttt attacacctg
     9661 ataacggaac catttcgtta attgctgatg ttttgcccga agtggttcgt gcaattcctg
     9721 ttgatgacac ccgtagtttt ggttcaatcg attcggtaac cctggctttg gaggcgctac
     9781 tttccgaaaa tggccaaacc gatttcgcca ttgaagcatc gggtattaag cggtttacgc
     9841 caatcctacc agtccctgaa aacgatggga ttactggaag cgttatatat attgattcct
     9901 actttaatgc aataaccaac atcactcgcg acgatttcga agcaattcgg aagtcccgaa
     9961 agtactccat atatgttcat cggttctcgc atggaataag caagttaagt aattcataca
    10021 aggatgttga aaagggcgat ttgcttgcgc ttttcaactc cttgggtttg cttgagatag
    10081 ctgtccgtga agggtttgcc gctcaaatgt acaacctctc agttggctca aatataatgg
    10141 ttcgttttta cgacgataaa attttaaggt gatgaatgat agcgttagaa aacaacgtgc
    10201 aagcgaagct tttcaaagat taatcggaat catggacgac ttgcgcgcca aatgtccctg
    10261 ggatagggaa caaacatttg aatcaatccg cccattaacc attgaggaaa cctatgaact
    10321 ttccgaggga attctggaaa aggattacaa tctaatccgc aaggaacttg gcgatttgct
    10381 gctgcatatt gttttctact cgaaaattgc agaggaggat aaccggtttg atattgttga
    10441 tgttataaac tcgctgtgcg acaagcttat atatcgtcat cctcatattt acggcaatgt
    10501 taaggttaac gatgcgagtg aggttgttga gaactgggag cagctcaaaa ttaaggagaa
    10561 ggatggcaat aaaactgttc tttcaggggt tccaacggct ttacctgcac tcataaaggc
    10621 caatcgaatt caggaaaaag tacgggccgt tgggttcgat tgggatgagc gttcccaggt
    10681 ctgggataag gttcaggaag aactcaatga gctcaggcat gaggttgagg taaccaagtc
    10741 caccgatgac attgagaaag aatttggcga tttacttttc tccgtggtga atgccgctag
    10801 gctttatggg gttgaccccg aaaccgcgct ggaacgaaca aaccgaaaat ttatgaaaag
    10861 attcacctac ctggaacaac aaaccatcca gatgggacgc tcgcttaagg atatgaccct
    10921 tgatgagatg aacgagattt gggaagaggc taaaaggttc gattaacata taaagtaaat
    10981 gttcctatac cttgttcgaa aattgtttta cacgttcctg gtgatttttg gcgttgccag
    11041 tctggttttc attcttttta accttattcc tggcgatccc gctcgcatgg ttcttggcca
    11101 acgaaccgat tcgctatcgg taaaggcagc acgtaccgat ttaggcctcg accttccggt
    11161 ttggaagcag tacttgaagt atttaaacga catttcgccg ctatcggtgc ataatccaag
    11221 taatagtaaa agttccattt acctcgacac acttatttat gcgaaaccct tagtgcttgg
    11281 gaaaatggca tcgaacaggg tattggtagc aaaacagcca taccttcggc gttcatacca
    11341 aagtcgtcaa cctgtttcca cagttgttat gtcaactctg cccaacacat tctatcttgc
    11401 actggtggca atagtctttg caacaattgt tggaattgca atggggataa ctgccgcaat
    11461 ttacaaggat agcgtttggg ataggttgct gcttgttaat tctgcgctag gtatggcttt
    11521 gccatctttc tttgctgcca tacttatagg ttggcttttt gccatagtgc ttggcaagta
    11581 tactggactt aaccttaccg gtaatctttg ggaggttgat gatttagggg aaggaattca
    11641 tcttaaatgg caaaacatca ttttgcctgc attaactctt ggcttacgtc cacttggcat
    11701 aatcacccag ctaatgcgca gttcattgct ggaaacatta agccaggact atatcagaac
    11761 agctaaagca aagggattgc cgaataggaa ggtgatcttt aaacatgctc tccgtaattc
    11821 ccttaaccct gttgtaacat ccatttcggg ttggtttgcc tcaatgatgg ctggtgttgt
    11881 ttttgttgag tatatcttta gctggaaagg acttggttat gttatggtaa acgcgcttaa
    11941 tagttacgat gtgccccttg ttattggctg cgttcttacg gttgctgtaa tttttagcat
    12001 agtaaacctg ttggtcgata tactatatgg tattctagac ccgagggtca gaatcggctg
    12061 atgtatcgga attttgttgt aaatttgata gcgaaaggtt aattaatgct tgaatgttta
    12121 tacttaattt aaatcgattg cgatgagaag aaaaattgtt gctgggaact ggaagatgaa
    12181 tacattgcct tccgaaggtt tggctcttgc caaggatgtt atagcaaaat cagctgaggt
    12241 ttcaagcggt gttgagctgg tagtaatccc accattcact cacctgaacg aggtaacaaa
    12301 actagccagc ggaacccgtg ttgcagtggg agcccagaat tgtgcacgct ggcaatcggg
    12361 tgcttacacc ggcgaggtgt cagctgccat gcttggcgct atgggagttg agtatgtaat
    12421 tatcggacat tcagaaaggc gcgaatactt tggtgaaacc aattcccagc tttacgataa
    12481 gattcagcaa gccctttcaa gcaatttgat gcccattttt tgctgtggcg aaaaactgga
    12541 tgaacgcgaa gccaatcaac attttcaggt tgttaagcaa caggttgagg aatcgctatt
    12601 tgcgctcgat gagcaaagca ttaagcaggt tgttattgct tacgaacccg tttgggcaat
    12661 tggcactggt agaactgcca ctgccgaaca ggcacaggaa atgcatgcct acatccgtaa
    12721 actgcttgct ggcaagtatg gctctcaaat tgctgactct attagcatcc tttacggagg
    12781 tagctgtaaa ccgtccaatg ccgctgaaat ttttgctaaa cccgatgttg atggcggatt
    12841 aattgggggc gcctcattgg ttgcagctga ctttattgct attgcaaaat cgttctagga
    12901 tgggttacac cgaactcaaa ataaggatca aaccatacac agccgaaaca gctgaaattc
    12961 tcattgccca acttgccgag ttgggctttg agagcttcat ggaaaatgaa cccgaactac
    13021 tggcgtacat taatactgca actctaaaaa aattacctgc cgattttatt ggccttattg
    13081 agttgcctga gggtgttaga attgattatg agctgaacag tattgccgac aagaattgga
    13141 acgaggtgtg ggagtcgaat tttgagccaa tcattgtcga taacagatgt ttggttaagg
    13201 catcgttcca taccgatacc ccaaaaaccg attacgaaat agtaatcgac ccaaagatgt
    13261 cgtttggaac gggtcaccat caaaccacac acttgatgat tgaggcgctt ctcaaccatc
    13321 aggttgaggg gcttaccgtt ctcgacatgg gttgtggaac aggtgtgctt gccatacttg
    13381 ccgaaatgcg cggtgcgcaa aacattgtgg ctatcgataa cgatgaatgg gcttaccgca
    13441 acacgctgga aaacattgac aataatagct gcaaacgcat cacggcctat ctaggcgatg
    13501 cagagctgct taaaggaaga tcattcgatt tgatacttgc caatatcaac cttaatatcc
    13561 ttttagccga tatggctgca tacgttaaca gccttaaaag tggtggagtt atattcatga
    13621 gtggcatttt aagggatgat gttgaaacgc tcaggaatgc tgccgaaagg ttaggcctat
    13681 cgtttagtga ggttaattat agggataatt gggccatggt tgctttccgt aagtagtttc
    13741 tattatgtat cggctttcat atggctaaac cttatgtata tagaagaata ttgataattg
    13801 cgctgctgct taccgcattt agccttctgt atgctgtgtt cattagccag gacaagaagt
    13861 tttccggtca ggtacccctt tttcctgcgg aacttaattc gcttgtaagc gataaagtta
    13921 aggagcagga agagtttttt ttaaaggggt tcccggcatt ttggctttcc gatactatcg
    13981 aaatatcaaa aaaagaggca attattggta cggccaatca gattctcgaa gttttgcccg
    14041 acgataagtc ttatatacta agctatgtaa agctcatagc agatttttac cgcaaccagt
    14101 atgcaaaaaa tcagttcgaa acatggttta gctacttaaa catggcattg gctgatggat
    14161 ttattaaatc cgataatctc cgaacattga ttcgtttttc tggttcgctt atcgatagtt
    14221 cgtttatttc cataagtact tcccatacat ggaaggtatt acctgcaaat tcgtatacct
    14281 tcaccttaaa aaataatgat ttgctggtta gtttccagaa tgtcaaccta atttgcagga
    14341 acaaccgcac cgatagtatt tttataagag gaacatccgg taatctggat ataattaagc
    14401 agtcgtggga ggggaaaaac ggtaaggtta catggattcg ttcaaaattc gacgaatcaa
    14461 aaatttttgt ggcactcaat aggtacagaa ttgagcttag gcaaagtgaa tatacagccg
    14521 attctgtatt gttaaattat cctgagtact tcaagaaacc tattcttggc agaatagtgg
    14581 ataaggttac acctatatac cagtcgggaa tagttgatta ccctgagttt aacccctacc
    14641 agaaatggtt tgaggttaag aatatcttta aggatgttga ctattcagga aatttcagta
    14701 ttacaggttc caagttagtt ggaattggcc ccgatggagg tttggccaag gtaacaattc
    14761 accgaaaggg aaaacccttt atggtggccg agggacgaat ggttttaatt gagcagtcgc
    14821 acttaagctc ggataaagcc aaggttgttt tttacattga taaagactcg atttaccata
    14881 acggtttgtc gtttgcatac cataatccta caagaactgt aatggtaaat ccaaccgata
    14941 aactaaccac tcaaagtcct ttctacagct cataccataa aataaacctt tggtcaaacc
    15001 agctaacatg gagtattgaa aaggatgaaa ttacctttgg tagcagcttg ggcgcttcaa
    15061 tatcaaaggc tgagtttgag tcggagaact tttttaatca agacatgttc gatagcatga
    15121 tggatgccag cgagttccat cctgtgctta ccgtttggaa ctatactcgt aggattaaaa
    15181 gtaacacttt tttggcatcc gatttggctg tgcatgtgcg cagagctccc gaggacgtta
    15241 aaatagccat gatgcgctta gccaagttag ggtatgtgct ttacaatttt gaaaccgacg
    15301 aggttaccat caccgataag ttaaagtata atgtgatggc ccgttttggc cgaaccgatt
    15361 ttgatgtcat caggtttcaa tccactactc ccggtacaca gcccaatgct cgcctcgatt
    15421 taaataactt aaatcttgcc attgaggggg taaactacat ttctgtgagc gattcgcaaa
    15481 acgtatttat ttccccgtac aagaaaagta ttaccttcca gaaaaacagg aattttgagt
    15541 ttggaggtag cattagggct ggtttattta ccttttacgg taaggcattc aggtttgact
    15601 attcgcagtt taaggttgag ttgaataaag ttgattcgct gattattgat taccaaaccg
    15661 attaccgcga taactacggt cgaaggatat tgcagggagt tgccaatgcg cttcacataa
    15721 tctcaggcga tatacttatt gataaaccct acaataagtc gggtagggag tacaacccgc
    15781 agtaccccat attcaactgt accagcaagt cttacgttta ctacgatgca tcttacatat
    15841 acgggggaat ttataaacgt gattcctttt actttgaaat tcagcctttt gtttaccaaa
    15901 gcatggacaa ctttgaaaag gccgacatga atttcaaggg aattctgtac tccggtaata
    15961 ttctcgcccc cattgaggaa acgctccgta tacgtcccga taactccctt ggttttatca
    16021 ctactactcc ttccgagggt atggctgtct ataaaggtaa ggggagggtt ttcaataaaa
    16081 tcgacttaag caatcagggg ctttttgtaa atggtagtat tgattatatt acctcaacta
    16141 cacagtccga taaaatgctt cttttccccg actcaatggt tacggtatca acgggctttt
    16201 ccattgcaaa aagggaaact ggcattgagt tcccaaatgt caagggcaat aaacatagca
    16261 tcaaatggct acctaaggct gataaaatgt ttatttccaa aggtgaaaaa ccctttgtga
    16321 tgtacgatag cttagcaact tttagtggaa accttctgct tcaaccttta ggccttactg
    16381 gagcaggttt gctgaatgtc cccaatggta aactttcctc atcgctattc gagtactcct
    16441 caaatgcatt agccgctaat gagtcaacac taactattct taaaactaat accgatagtg
    16501 ttgcccttga aacctcaaag gttaaagcta ctataaattt cgcaaacatg cttggcgagt
    16561 tcagccgagt tgacaaaagc atttatgcct cgctcgatgc tttaaggtac agcacctatc
    16621 acgatcgttt taagtgggat ctcaaaggat acaaggttta ctttgaaaca ttaatggctc
    16681 aggaagatgt tttggccgga aagtttaggg ttagtaatat gatagatagg gacagcatac
    16741 cccgtggaaa tctgtttttc agtaccaagc cggccgagga ttcactatac ttcttcgcac
    16801 caaaggctgt atataatact gatagaatac atttggtagc cgatagtgtt aagtatattg
    16861 taactgccga tgccactgtt ttcccaaaaa ataaaagggt tgaggtagat gttcccaacc
    16921 gtatgtataa gttctccaat gcaaaggtaa gggctaacaa tacaacggcc taccatgtat
    16981 tgcatgacgc tgagatcaag attaacggac ggaaaagcta cggtggttcg gcatactacg
    17041 attacgttga tgaacgcgat tcaatccaaa ccatctactt tccaaaagtt tactctgata
    17101 acaatatgaa tacctatgcc gagggtaccc taacagagcc cgatagtttt aggttaagtc
    17161 ccaattttgc tttcattgga ggcgttagcc ttagtgctcc tgaaaagttc cttttcttta
    17221 agggtggggc aaaaccaatt tacaactgcc ccggaactat tggccagtgg gtacgatttg
    17281 aatcgcaaat caaccccaag cgggttttaa tcccaattgg tcctgatcct cgcaacatga
    17341 acctccatcg cttaattagc ggaactgcca ttgccgagga ttctttaaaa ttgtatggtg
    17401 gtcttttgcg cagtcgtaaa ttctgggagg attcatcgct ggtaacagca accggattca
    17461 tggagttcaa caataaaaac cgccggttta ccattgcacc cctatacaag ttagataatc
    17521 ccgattcttc tgggaatagt gttagcctcc aaaaggacta ctgctgggct tttggtgagg
    17581 gcaacgtgcg gttaccccta aagttaggcc atgttacctc gcaggtttac ggtagtgtaa
    17641 tacataaact ggacaaaaat gaggctacaa tcgatgcgat tatcaggttg aactttcatt
    17701 tcaaccaaaa ggcccttgag gccatggctg ctcagctaag tggcgccatt actctcgata
    17761 aggttgattt aagccgtaag ctttaccgta aaatgcttta cgattggctc acacctaagg
    17821 agttgcccga ggcgttaaac caactttcgc tttttggttc attcacacag atgcccaaaa
    17881 gcctggagag taccattaca ctttccgatg taaagttgag gtgggatcct cagaataaat
    17941 cgtttgtgtc agtcgggaaa attggagtgg gtactattgg taatattcag gtaaaccgtt
    18001 ttgtgaatgg gttcattgag tattacaagg gacgctttaa cgataggtta atcctttaca
    18061 tccatattgg cgacaacgag tattactcgt tctactatgc caatggggta atgtttgtca
    18121 gtgcaaccaa tcctgaattt tataacccta ttgcctccca aaaatcgagt gagcgcaggg
    18181 taaaaccatc aaaaggaatg atgggctata ggtatatgat tggatcaaaa cgtgaactaa
    18241 acagagctca gctccgctat aaggagttag cctttggcat taagtccgat attccggttg
    18301 attccgatga cgacaaacca aataaggagg gcaaagaaga cgattaaaag cccctctttt
    18361 tttcccaaac ctccagatca gaaatattag aaccatcaat ttttagtcta atcgctgttt
    18421 ttacctgatg gaagccactg tttccagccg ctcccggatt tatgtatagc atatcccatt
    18481 gcttatcata cttaacacgc agtatgtggc tgtgaccaca tactaagatg tttaccttgt
    18541 ttcccttaag gatttccaga actttttgat cataatgtcc tggtgtaccc gcaatatggg
    18601 taatcagtat gttaacattt tctgttgtaa acagttgttt ttcgggataa accagtcggg
    18661 ttttgtaatc gtcaatgttg ccatgaactg cacgcaaggg ttttagcttt accatattat
    18721 cggctagctc aagcgatcca atatcacctg catgccaaat ctcatcgcat gtttcaaaaa
    18781 aatcggtaag ccctttgtcc caaaatccgt gtgtatccga taggatgcca atagctttca
    18841 tctttttatt ctgaaattca ctgactcaaa attaaaaact tcagtctaaa ttgtaaattt
    18901 gcatcaacgc tgattaaata atctagcact tgttctattt atgttttttt atcaatctgt
    18961 acaccctttg cagatggcaa tttaactatt aaaggtttta tacgattttt tgttatgttt
    19021 aacttgacaa aaacaattgg gttcattaat ctcaaatctc aaatcttgaa tctgaaatct
    19081 gaaatctgaa atcttgaatc tctaatccca tgcagatatt ttatgcccca caaattgaag
    19141 gcgatgagta taggctacct gaggacgagt ccaagcattg tattcgggtg ctaaggatgc
    19201 agcaaggcga cgaactttac cttaccgatg gttgcggaaa tctgcataca gcgcgtattg
    19261 ccgatgaaaa ccccaagcga tgcttagttc aaattgtttc aactcagcat gagtacggta
    19321 aaaggccatt caatctgcat atcgccatcg ccccaactaa gaacatagaa aggtttgaat
    19381 ggtttttgga aaaggccacc gagattggtg ttgataccat tacccctctc ctatgcgaga
    19441 ggtcggagcg taaagatatt aaggttgaaa ggttgcaaaa aattgtgatt tcagccatga
    19501 agcaatcggt aaaggcctac ctgccaataa ttaatagtat tcaacggtta agggacttcg
    19561 ttaaacatac gcaccaagcc gatttaaaag caatagcgca ttgtaatagc tgggatttga
    19621 ctccgtttca gcaggtaatc cataggggta ggagtgttct tgtcctcatt ggtcccgaag
    19681 gcgatttcac gccagccgaa gt
//
LOCUS       DAOOWJ010000045        19407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2818_length_19407_cov_5.930136, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000045 DAOOWJ010000000
VERSION     DAOOWJ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_19407_cov_5.930136"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..403)
                     /locus_tag="PLW22_06065"
     CDS             complement(182..403)
                     /locus_tag="PLW22_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOU74304.1"
                     /translation="MYWTLELASKLEDAPWPATKEELIDYAIRSGAPLEVIENLQELE
                     DDNEIYESIEDIWPDYPTKEDFLFNEDEY"
     gene            complement(486..1034)
                     /locus_tag="PLW22_06070"
     CDS             complement(486..1034)
                     /locus_tag="PLW22_06070"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOU74305.1"
                     /translation="MKIYTKTGDKGSTSLIGGTRVPKYHPRIETYGTVDELIAFTGLL
                     RDQDIDRHTVETLIFIQDRLMNCASILAADCTDCKVRIPKIESADIQRLENEIDAMDA
                     QLEPLTSFVLPGGHPVVSVCHVCRTVCRRAERLVTHLAEETPLPENLVVFLNRLSDYF
                     FTLSRKLGKDFGVEQIKWQPNL"
     gene            complement(1112..>1387)
                     /locus_tag="PLW22_06075"
     CDS             complement(1112..>1387)
                     /locus_tag="PLW22_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU74306.1"
                     /translation="QRVAVARALVNNPSIILADEPTGNLDSKTSIDIMNLFEDIYEQG
                     NTIIVVTHEEDIARHARRIVRLRDGHIESDEPNPNPILRQKVVITEQ"
     assembly_gap    1388..1487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1597..2406)
                     /locus_tag="PLW22_06080"
     CDS             complement(1597..2406)
                     /locus_tag="PLW22_06080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU74307.1"
                     /translation="MRKGALGILMNYLHFRKKCLPKGGYGIHSPFVFDLYTTTIENDS
                     RESVFESIEVYRKELLGCDIGIIRKSIAESLNGGFRGELVKLSRIARTASVPPHLGRL
                     LYRIVNRFKPNVMLELGTSVGISSMYIAKANPQGVLYTIEGDEAVFGIAQKRFDTMNI
                     TNIKPICGIFETELPSIAREVPGFDFVFIDGDHNGTKLLQYFNVILPKLNENSVVVVD
                     DIRWSNDMEDAWRELKANRQVSVSFDLFRCGVLFFRKGIAKQNFMLRYGPF"
     gene            complement(2498..2965)
                     /locus_tag="PLW22_06085"
     CDS             complement(2498..2965)
                     /locus_tag="PLW22_06085"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HOU74308.1"
                     /translation="MERYLKALQRGIIYFTLFIISLLLILALIDVFIKLYDGLINNPL
                     RTFIELQYPQLIDLFLVIIIGVELLETIKGFLRDEILHVELVVLVAIIAISRKVIVWG
                     ISKTTSQEMFSLAAMLAALALTYWVIKTCYKKKYNKLGSNLPKANSSCGDVNV"
     gene            complement(3048..>4154)
                     /locus_tag="PLW22_06090"
     CDS             complement(3048..>4154)
                     /locus_tag="PLW22_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU74309.1"
                     /translation="KPNLLLSVPALAKNFKKNIENGIRAKGKFTEMLFNHALKISYKY
                     NKEGWNKGTGFTFIYKPLIWLYDKILFSKIREVFGGELDFFIGGGALLDIELQRFYYA
                     IGIPMLQGYGLSEATPIISSNSLKRHKLGSSGHLVKYLELKICDENGNELPVGEKGEI
                     VVKGENVMVGYWKNEKATAETVRDGWLYTGDMGYMDKDGFLYVLGRFKSLLIASDGEK
                     YSPEGIEESLVQHSKFIDSVVLHNNQKPYTTALIVPSKEALKKAITAKGIDPESHEAA
                     KEAIAIINNEIARFKPGGEFGDMFPERWLPTTFAILDEPFTEQNGLVNSTLKVVRGKV
                     EKLYENRLEFMYTPSGKNIVNDANIQSVQNLLKN"
     assembly_gap    4155..4254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4255..4713)
                     /locus_tag="PLW22_06095"
     CDS             complement(<4255..4713)
                     /locus_tag="PLW22_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU74310.1"
                     /translation="MKTIIDLFEESVSKFSDNPYIWEKVTDRYEPTSYAQTRTEVYRL
                     AAGLLSLGVKKDDKIALLSEGRKLWVISELGILYTGAVNVPLSVKLDGNDLLFRLVHS
                     ETSMIFVSGQQASKVEKLRSQLPNVKRVIYLDQPERLEGDDLFIGDVFKLG"
     gene            4815..5738
                     /locus_tag="PLW22_06100"
     CDS             4815..5738
                     /locus_tag="PLW22_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOU74311.1"
                     /translation="MDENKIFDLSCCTLCPHECGIDRYAHTGFCNVGVNPVVSSIFPH
                     KGEEPVISGEKGICNVFFAHCNLQCIYCQNHQISRNTKHSKKWLTDNKVIVDEIKSIL
                     NTGIGMVGFVSPSHQVAQMVDIVNSLNKSGYKPIIVYNSNGYDKVETLQMLEGIVDVY
                     LPDLKYYDSILAQRFSGVSDYFQLASKAIKEMYRQKGSTLILNNEGLAEFGMIIRHLV
                     LPGHANDSIKLLNYLAEEVSTKLYISLMSQYYPPAELTLPNELNQHVGIDEYKKVIEV
                     LENHGFRGWVQDLVSSHFYRPDFESNNPFSE"
     gene            5854..6306
                     /locus_tag="PLW22_06105"
     CDS             5854..6306
                     /locus_tag="PLW22_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74312.1"
                     /translation="MKRIILFIVLAAFVGTGCKYFKKDKKVDPIALQKAKEDSIAKAK
                     AFEEEQKRIAEEQARQEELRKQQEYEATYRFHVIIGSFKVPSNATAWEEEVHKLGFNK
                     TKILDSPNGFKLVSIGQYDTYSKAFNEIERINSERPDEPMEMWVYENK"
     gene            6440..7837
                     /locus_tag="PLW22_06110"
     CDS             6440..7837
                     /locus_tag="PLW22_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU74313.1"
                     /translation="MEKIRQTLRDHAWARWTALAVVAFTMLTGYYLTDVMAPLKGLLE
                     QQLSWDSSEYGLFTSAYGWFNVFLFMLIIGGIILDKMGVRFTGLMAATIMVIGTSIKF
                     WAVSTHMLDGQVWNILWIFPIKAQVFMAALGYAIFGVGVEVAGITVSKIIVKWFKGKE
                     LALAMGLEMATARLGTALALATSVPIAKAFGHVSKPILFCLILLVIGLISFIIYTFMD
                     KKLDASQAEYDKSNNVTNEEEAFKISDIFNIITNKGWWYIAILCVLFYSAVFPFLKYA
                     ADLMVNKFGVDESLAGTIPAMLPFGTILLTPFFGNIYDRKGKGATIMLIGSLLLIFVH
                     TMFSIPFLNHWIIAIVLIIILGIGFSLVPSAMWPSVPKIIPENQLGTAYSLIFWVQNW
                     GLMGVPALIGWVLDKYCVTGQKVVEGVTVNTYNYTIPMIIFTSLGILALVFALLLKAE
                     DKKKGYGLELPNIQK"
     gene            7971..8525
                     /locus_tag="PLW22_06115"
     CDS             7971..8525
                     /locus_tag="PLW22_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74314.1"
                     /translation="MEMQTVDESTTLISTIVSFTAIQDQISNTLSINFPKAQEVIINR
                     SKARITDSKGNVIWKGSLEKKVTSENTLNFKFGKDWLNKKGAQPYTIEWETNVLVSSP
                     TGIYVWPACLSGNINSVKYLGLRVFADNEFREKIETNLEVSSRLMDQSSGLSGVVWEL
                     SNYRTKKAWQYTKPIENPFVKISF"
     gene            complement(8533..9801)
                     /locus_tag="PLW22_06120"
     CDS             complement(8533..9801)
                     /locus_tag="PLW22_06120"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOU74315.1"
                     /translation="MSKVDILLGLQWGDEGKGKVVDVLTPNYSVIARFQGGPNAGHSL
                     EFNNIKHVLHTIPSGVFHPGCLNLIGNGVVIDPIILAEEIHSLEKLGVNLKKSLKISK
                     KAHLILPSHRELDAASEAAKGKGKIGSTLKGIGPTYMDKTGRNGLRVGDILLPDFKDR
                     YNFLKNKHKEILRQYNYSSNIEQHEEEWFKGIELLRQYDLIDSEYEINSYIKQGKRVL
                     AEGAQGSLLDIDFGSYPFVTSSNTICAGACTGLGIAPHAVGEVYGIFKAYCTRVGSGP
                     FPTELDNEIGEKMRKIGVEFGATTGRPRRCGWLDLVALKYAIMLNGVTQLIMTKPDVL
                     DSFETIKVAVAYKVNGIETDRLPYDINTPIEPVYKEFKGWMTDISGFKSDSDLPDELM
                     AYIQFIEQETGVPVRIISVGPNRDETIVRE"
     gene            complement(9935..10408)
                     /locus_tag="PLW22_06125"
     CDS             complement(9935..10408)
                     /locus_tag="PLW22_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOU74316.1"
                     /translation="MTCSDTKLVVKRILTDYLERKGHRKTPERFAILDEIYSQESHFD
                     IETLYIKMKNKNYQVSRATLYNTIDLLLECGLVIKHQFGKNSSLYEKAYECKQHDHLI
                     CSRCGKVIEFCDPRIQEIINSAQKNSGFTISHHSLYLYGLCKDCQTEIDKVDLSL"
     gene            11122..11451
                     /locus_tag="PLW22_06130"
     CDS             11122..11451
                     /locus_tag="PLW22_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOU74317.1"
                     /translation="MPRSKCPRRICHSPDIHLFKPAGKPLHLMEIVVLEADELEAIRL
                     ADGIGLYHEEAAKQMGVSRQTFGRILEQARSKVAKALTNGMALRINKSQTEIFCDQQN
                     NQNSEHI"
     gene            11490..11879
                     /locus_tag="PLW22_06135"
     CDS             11490..11879
                     /locus_tag="PLW22_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOU74318.1"
                     /translation="MKIAVPTRHGHVDEHFGHCEFYTVYTIENGKVTAKETLGSPQGC
                     GCKSNIAFDLQQMGVEIMLAGGIGSGAINVLAAHGIQVVRGCSGNTDEVVDLYINGEL
                     TDSGESCSHHEHHHGSHQGEHGHSCNH"
     gene            complement(11976..14213)
                     /locus_tag="PLW22_06140"
     CDS             complement(11976..14213)
                     /locus_tag="PLW22_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOU74319.1"
                     /translation="MSLQTDEEKLLIQSHFEDLLNSCTRCNSPEDKELIVKAFNLANE
                     AHKGVRRKSGEPYILHPIAVAKIVTSEIGLGAKAVACALMHDVVEDTDYTIEDIERIF
                     GKKIASIIDGLTKITDVFDANSTIQAENFRKILLTLADDIRVILIKLADRLHNMRTLE
                     SLPATKQMKIASETIYLYAPLAHRLGLYAIKTELEDLSLKYRYPQVYEEIRSKIADNE
                     KRRVQLINHFSLPIIQKLEENNIDFEISGRPKSVYSVWKKMQTKNVSFEEIYDLLAVR
                     IVFNPYPHIPEKTQCWHIYSLITDIYKPKPDRLRDWVSTPKANGYEALHCTVMGPDGK
                     WVEVQIRSRRMDENAERGFAAHWRYKEMDATSQENELDKWIRRVREMLENPQENALEF
                     LDEFKLNLFTSEIIVFTPKGETRSLPKGATALDFAYEIHTEVGNRAIGAKVNHRLVPL
                     SYQLNTGDQVEIITAASQKPTWELLNFATTAKAKAAIKNALKGEVKNRIEKGQQLLEE
                     KLLEMGIQPSSRVFKKILPAYEVSSKDELYAKIGSGLINLDNLKKILKKNTQSKWIKY
                     WKLQLGFGAKKVEPESEIQIKYDLKKPIVLGADTDSDNPSYLVANCCNPIPGDEVIGY
                     LSPDDIVIIHKATCAEAVRLMAQHGDKIVAAKWTTQKALSFLARVEVNGMDRIGIISE
                     LTKVISDELMVNIRKFHIESHDGIFEGFIDLYVHDVTHLNNLILNIMKVKGMENVRRM
                     DKFDD"
     gene            complement(14229..15842)
                     /locus_tag="PLW22_06145"
     CDS             complement(14229..15842)
                     /locus_tag="PLW22_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU74320.1"
                     /translation="MKNIRQVILMIGLTFYMPTLWAQFSNKPNPPTLDYITNNLDNGD
                     VTIFWTKPPHEPQIADPLGYIIYVPRNPNDPDADGWDSIATVDQNTFQYTHIGANGNG
                     QRVYYRVALLGASEPSQMTVKHSQVWITASYDSCNAKIDISWVGYEAWPLNASSPYRN
                     IRYQLYMGNDLNWSAYTQIGDYEGYSNRHSVRDVQENQDYFFYIKATRMDTGYESYSN
                     LYLIHTKMARRPTYVSVDSVIATNNGNRIYYTIDPNSRVIDYKLWRWEQPDTAQSIFS
                     AKTIEGFDVPTKSMSIDTNDTWAARTRTFYYKVDAYNGCNDAVLVSNLCNTIIPRLSP
                     KGNVVSIQWDPLILDTQRKPDRAGNRIEYSIYRRSFTVNDDISGPGNLERIANGLSVT
                     EYADDLSGFKGQSPLYRIRFNYYIEALELSPANDTVTLSRSREISTEILPGVTMPTAI
                     APNDPRSENGHERSLFKPVIDFEANFILTIYDRWGGVVYHGNQGWDGRNSRGEYVKEG
                     TYVYRLVVSTADAGDIIKTGSISVVYPKK"
     gene            complement(15848..>16864)
                     /locus_tag="PLW22_06150"
     CDS             complement(15848..>16864)
                     /locus_tag="PLW22_06150"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU74321.1"
                     /translation="FSRDFASVTFYTIGKHFYRSLDVLADILTNPTYPQHELELFCKK
                     GKQSLLVDLEKVSTLSRQRFFSALFGSNHPYGSFATPNDYDVLSQTVLVQFRNRYHKA
                     SNGLIVIAGKVDAKQVDFLVNGFNNPIFEQCDDIELGLPSFTTTDYRVFSYKKDAVQA
                     ALRIGRVLPKRNHPDMPGLVVLNTILGGYFGSRLMRNIREDKGFTYGISSFIIPMAEL
                     SALVVSTEVGSAFTEKTVDEVFKEMIKLQTEPVSNDELELVRGYMTGQVLRTFDGPFA
                     IADSLASLFQYNNLDFEYINNLIRTINEITPTQLLGLAKKYLDVDRMVVSIAGSKRIR
                     GYKE"
     assembly_gap    16865..16964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16965..17791)
                     /locus_tag="PLW22_06155"
     CDS             complement(<16965..17791)
                     /locus_tag="PLW22_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU74322.1"
                     /translation="MPQNIDIAFKSLIIIPKTSTKIVNIEDIFVSMIDFVKYKLDNGL
                     VLIVHTDYSTPIAAFNLLYSVGSKNEDPERTGFAHLFEHLMFGGSANIPVFDEPLERV
                     GGENNAFTSNDLTNYYITVPTENIETAFWLESDRMLSLAFSQKSLDIQKSVVLEEFNQ
                     RYLNQPYGDIWLYLRPLAYKVHPYRWPTIGQSPEHISRATLADVKNFFFSHYAPNNAI
                     LCVAGPVDPNEILELTHKWFGSIEPRKLANKSIPREPEQTEPRFLTLEREVPFNAIY"
     gene            17819..>19407
                     /locus_tag="PLW22_06160"
     CDS             17819..>19407
                     /locus_tag="PLW22_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOU74323.1"
                     /translation="MLREGFKISLTKFWAIFRSLGERRITLILSFIIGLLSGVAGVAL
                     KNTLHGVRNFVNGLVPMDTASLLYFGLPAIGILLTLLFVRYLIKDDLSHGVTKVLYSI
                     SRQGSKLKAHHMFSSIFGSALTIGFGGSVGAEAPVVLTGSAIGSNVGSFFRMNYKIRT
                     LLIGCGAAGAIASIFKAPIAGIVFTLEVLMLDLTTASIVPLLISAVTASSVSYLLMGR
                     NVVFAYDVVRPFALHNIPWFILLGIFCGLVSLYFIRSIWSIEKRFKQTRSPYKKWIVG
                     TIILGALIFVFPPLFGEGYDVLQALLNGQSEYIFHNSPLYGFQNNYWLLIGSLFLIIA
                     LKAVATAVTNGAGGVGGIFAPSLFIGGISGFLVAKLINQFNFIDVSESNFALVGMAGT
                     MAGVMHAPFMAIFLIAEITGGYALFVPLMITSTIAYLTIMYFEPHSIYTKRLATRGEL
                     ITHHKDKAVLTLLNVSKVIETDFIPVEPTETLGSLVKKVSQSRRNVFPVVTTEGKFLG
                     MVMLDDIRPIMFDASRYDTTTV"
BASE COUNT         5440 a         3988 c         3990 g         5689 t
ORIGIN      
        1 tggaagatgt tatgcttaag ggtttcaaat ggctcgaggc taaacgaccg gctaacaggt
       61 acagcgtaat acctaaggat tttaaagagc aacctgagga acgtggtaat aaaaagcaac
      121 gcgaaatact cagcagtaaa aataaataag caaaaaggag aggttgacct ctccttaatt
      181 tttagtactc atcctcgttg aatagaaaat cctctttggt gggatagtcg ggccagatat
      241 cttcaatact ttcgtaaatt tcattatcat cctccagctc ctgaagattt tcaattacct
      301 caaggggagc accagaacga atggcgtaat caattaattc ctcttttgtg gctggccatg
      361 gagcatcttc taacttggat gctaattcta atgtccaata catagtgctt ttaagtttat
      421 taaaaaactg attgtcagta aaataacttt attgacaacc acacaaatat ataaattttt
      481 tggaattaca aattaggctg ccattttatt tgctcaactc caaaatcctt gccaagtttc
      541 cgtgaaaggg taaaaaagta atcgctaagc cggttaagga aaactacaag attttcaggg
      601 agtggtgttt cttcagctag gtgagtaacc aaacgctcag ccctgcggca aacggttcgg
      661 caaacatggc aaaccgaaac aaccggatga cctccaggta gcacaaacga tgttaaaggc
      721 tctagctgag catccatggc atcaatctca ttctccagac gctgtatatc ggcgctttca
      781 attttaggta tccttacctt gcaatcggtg cagtccgcag caagtatcga tgcacagttc
      841 attaacctgt cctgaataaa tatgagggtt tcaacagtgt gtctatcaat atcctggtcg
      901 cggagcaaac cggtaaatgc aatcagctca tctactgtgc cataggtttc aatccgtgga
      961 tggtacttgg gaacgcgagt tccgcctatc agcgatgttg agcccttatc gcctgttttt
     1021 gtgtaaattt tcatacaata attgttgcgt tgcaatatag tttatctacc ttgtttaaac
     1081 aagaaatatt taaatcaacg caaaccaata tttattgttc agtaattaca actttttgtc
     1141 tgagaatggg gtttggattt ggttcatcag actcaatatg accatcgcgg agcctaacaa
     1201 ttctccgggc atggcgggca atatcctcct cgtgagtaac aacaattatg gtattgcctt
     1261 gctcatagat atcctcgaaa aggttcataa tatcgataga cgtttttgaa tcgaggttac
     1321 cggtaggctc atcggcaagt ataattgacg gattgttaac cagggctcgt gccacagcca
     1381 ctcgctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc ttttaaggct
     1501 ttcacaacaa cgcttcctac ctggtagtac tttttaatgt ttgtaatgtt gattaagtaa
     1561 tgctcgctca tatactaggt tattatattt tacttgttag aatggtccgt atcgaagcat
     1621 aaaattttgt ttggctatgc ctttcctgaa gaaaagcact ccacacctaa acaggtcaaa
     1681 ggaaactgac acttgcctgt tggccttgag ttccctccag gcatcttcca tatcgttact
     1741 ccagcgaata tcgtcaacca caaccactga attttcattt aactttggaa gtatgacgtt
     1801 gaaatactgc aaaagtttag tcccgttatg gtcaccgtca ataaaaacaa aatcgaaacc
     1861 gggtacttcc cttgcaatac ttggcaattc tgtttcaaat ataccacaaa tgggtttaat
     1921 atttgtaata ttcatggtat cgaaacgttt ttgggcaatt ccaaaaactg cttcatcgcc
     1981 ttcaatggta taaagaactc cttgtgggtt tgccttggca atgtacattg aactaatacc
     2041 aaccgatgtt ccaagctcga gcattacatt gggtttaaag cgatttacaa tcctgtatag
     2101 caacctcccc aggtgtggtg gtactgaagc agttcgggca attctactta gcttaaccag
     2161 ttcccctcta aatcccccat tcaggctttc ggcaatcgat ttacgaataa ttccaatatc
     2221 gcagcctaac agctccttgc ggtacacttc aatactctca aagaccgatt ccctgctatc
     2281 gttttcaatt gtagtggtat acagatcaaa aacaaaaggt gaatgtatgc catatccccc
     2341 cttcggtaag catttcttcc tgaaatgaag gtaattcatc aaaatcccca aggctccctt
     2401 tctcattttc gcaattctat gaaatgctaa ggtaagcccc agggtaattt aaacctccgc
     2461 tggatgtgtt aaaaaaagtt tattatacct aattttatca aacattaaca tcgccacaac
     2521 tagaatttgc ctttggaagg tttgacccta acttgttata cttcttttta tagcaagttt
     2581 taataaccca ataggttaat gccagggcag ccagcatggc agccagggaa aacatctcct
     2641 gcgatgtggt ttttgaaatt ccccatacta taaccttgcg ggagatagca attatggcaa
     2701 ccaaaaccac aagctccaca tggagtatct catccctgag aaaacccttt atggtttcca
     2761 gtagctcaac cccaataata attaccaaaa ataaatcgat aagctgagga tactgcaatt
     2821 caataaaagt tcgaagtgga ttatttatca acccatcgta tagcttaata aaaacgtcaa
     2881 ttaacgctaa aattagtagc agcgagataa taaaaagggt aaagtaaatt attccccttt
     2941 gaagtgcttt aaggtatcgt tccatgcgtt aaagtctttt agtaaagtta aaaaaaagga
     3001 ggacatttaa gtgccctcct tacaacaaat ttgtgatgat atagttctta atttttaagt
     3061 aagttttgaa ccgactggat atttgcatcg ttaacaatat ttttgccact tggggtgtac
     3121 atgaattcca ggcgattctc atagagtttt tcaacttttc ctcttactac cttcagtgta
     3181 ctattaacta aaccgttctg ctcagtaaag ggttcatcaa gaatggcaaa ggttgtaggt
     3241 agccatcgtt cagggaacat atcgccaaac tcgccaccgg gtttaaaacg ggctatctca
     3301 ttgtttatta tggcaatggc ctccttggcg gcttcgtgcg attcagggtc gataccctta
     3361 gccgtaatgg cttttttcag agcctccttt gagggaacaa ttagggcagt ggtgtaaggc
     3421 ttttggttgt tgtgaagcac aacgctatca ataaacttgg agtgctgaac caacgactct
     3481 tcaattcctt cggggctata cttttcgcca tcgctggcaa taagcaagct tttgaacctt
     3541 cctaaaacgt aaaggaagcc atccttgtcc atgtatccca tatcgccagt ataaagccat
     3601 ccatcgcgca ccgtttcggc ggttgccttc tcgttcttcc agtaacccac cattacgttc
     3661 tcgcccttga caacaatttc gcctttttcc cctacaggta gttcattgcc attctcatcg
     3721 caaattttaa gttcaaggta ttttaccagg tgacccgatg aacccagttt atggcgtttt
     3781 aacgagtttg aactgataat gggagttgct tccgataatc catagccttg tagcattgga
     3841 attccaatgg catagtaaaa gcgttgtagc tcaatatcaa gtaatgcccc accaccaatg
     3901 aaaaaatcga gctcgccacc aaaaacctca cgtattttgc tgaacaaaat tttatcgtaa
     3961 agccagataa ggggcttata aatgaaagta aaaccggttc ccttattcca accctcctta
     4021 ttgtacttgt atgagatttt taaggcatgg ttaaaaagca tctcagtaaa ttttccctta
     4081 gctctaatgc cgttttcaat attctttttg aagttcttgg ctaaagcggg cacgctgagt
     4141 aaaaggtttg gtttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcctag
     4261 cttaaaaaca tcgccaatga acagatcgtc gccctctaac ctttccggct gatcgaggta
     4321 tattaccctt ttgacgttag gtaactgtga gcgtaacttt tcaacctttg atgcttgctg
     4381 tcccgataca aaaatcatag atgtttcgga atgaaccaat cggaatagta agtcattacc
     4441 atctagcttt accgaaagag gaacatttac agcaccagta tagagtatcc ccaattcact
     4501 tattacccaa agcttgcgtc cttcgctcag taacgcaatc ttgtcgtctt tctttactcc
     4561 aaggcttaat agccctgcag ctaagcggta aacctcagtc cgtgtttggg catacgatgt
     4621 tggttcgtac ctatcggtta ctttctccca aatgtaaggg ttgtcggaaa acttacttac
     4681 gctctcctca aaaagatcga ttattgtctt catcgtttca aaattttcac cattagcata
     4741 gacaaaacta taaaattttg ttttgcaact cacctaatca tatctaaaag tagtaggttt
     4801 agtatttttg tggcatggat gaaaataaaa tctttgattt aagctgctgc accctctgcc
     4861 cccatgaatg tggcattgat aggtatgctc ataccggttt ctgtaatgtt ggggtaaatc
     4921 cagttgtatc atcaattttt cctcacaaag gtgaggagcc agttatatca ggggaaaaag
     4981 gaatatgtaa tgtttttttt gcgcattgta acctgcaatg catttactgc caaaaccacc
     5041 aaataagtag gaataccaag catagcaaaa aatggttaac cgataataag gtaattgtag
     5101 atgagataaa aagcatactc aacactggta ttggaatggt tggctttgtt tcaccgtcgc
     5161 accaagttgc ccagatggtt gatattgtaa attccttgaa taaatcgggc tataagccaa
     5221 ttattgtata caactccaat ggatacgata aggttgaaac cctacaaatg cttgagggta
     5281 tagttgacgt ttacttaccc gatttaaaat attacgacag catattagcg caaaggtttt
     5341 caggggtatc ggattatttt cagttagcct caaaagccat taaggaaatg tataggcaaa
     5401 aggggtcaac cctaatccta aacaacgagg gtttagcaga gtttggaatg attatcagac
     5461 atttagttct gccaggtcat gccaacgaca gcattaaact tctcaactat cttgccgaag
     5521 aggtttcaac aaaactatac atctcactca tgtcacagta ttatccccct gcagagctta
     5581 cactgcccaa tgaattaaac cagcatgtgg gtattgatga gtataaaaaa gttattgaag
     5641 tattggaaaa tcatggcttc aggggttggg ttcaagacct ggtaagcagc catttttacc
     5701 gtcccgattt tgaaagtaac aatccctttt cagaataaga tacctgaaag ggataaaatc
     5761 agcacggttg cttgaacttt tagcattaaa ttttgattta ttgaaaattt gtatatatat
     5821 tgcactgaaa ttaaacctta accgagaaac cctatgaaaa gaattatact gtttatagta
     5881 ctggcagcat ttgttggcac gggttgcaag tactttaaaa aagacaaaaa ggttgacccc
     5941 attgccctgc aaaaagccaa agaggacagc atagcaaaag caaaagcttt tgaggaagaa
     6001 caaaaaagaa tagccgaaga gcaagcccgc caagaggaat tgcgcaaaca gcaagagtat
     6061 gaagccactt accgttttca tgtgattatt ggtagtttta aagtgccttc aaatgccaca
     6121 gcatgggagg aagaagttca taaattgggt tttaacaaaa caaaaatact tgactcaccc
     6181 aatggtttta agttggtttc aattggtcag tacgatacgt atagcaaagc ttttaatgaa
     6241 attgagcgta ttaactccga gcgacccgac gaacccatgg aaatgtgggt atacgaaaac
     6301 aagtaaatta cgattaagcg atagtaaaac cgccaatttt taggcggttt tttattttgt
     6361 tgacaaaaaa tgtatttggt ttgagctatt ttgattttat ttgctgcaca aaacaaacta
     6421 aacctaattt ttaaactaaa tggaaaagat tcgacaaaca ctgcgcgatc atgcctgggc
     6481 acgttggaca gccctggcag ttgtagcttt taccatgcta accggttact acttaaccga
     6541 tgtgatggcc ccgctcaaag gacttttaga acaacaactc tcatgggata gttcagaata
     6601 cggacttttt accagcgcct acgggtggtt taatgtattt cttttcatgc taatcattgg
     6661 cggtattatc ctcgacaaaa tgggtgttcg gtttaccggc ctaatggctg ccaccataat
     6721 ggttattggt acatccatta agttctgggc tgtttccacc catatgctcg atgggcaggt
     6781 atggaatatt ctatggattt tccccattaa agcacaagtg tttatggctg cattggggta
     6841 tgccattttt ggtgtaggtg ttgaagtggc cggtattacc gtttcaaaaa tcattgtaaa
     6901 atggttcaag ggcaaggagc tagcgcttgc tatgggtttg gaaatggcta ctgcccgttt
     6961 ggggactgca cttgctctgg ctacatcggt acccattgct aaggcttttg gccatgtttc
     7021 aaagcccatc cttttctgct tgattcttct cgtaattggc ttaatttcat ttatcattta
     7081 cacttttatg gataagaagc tcgatgcttc ccaagccgaa tacgacaaga gtaacaatgt
     7141 gaccaacgag gaggaagcat ttaagatatc ggatattttc aatatcatta ccaacaaggg
     7201 ttggtggtat attgccatac tttgtgtgct attctactca gccgttttcc cattccttaa
     7261 gtatgccgcc gacttaatgg taaacaagtt tggtgttgat gaatcgcttg ccggtacaat
     7321 acctgccatg ttaccctttg gtaccatact gcttactccc tttttcggaa atatttacga
     7381 caggaaaggc aaaggggcaa ctatcatgct gattggctca cttctgctta tctttgtgca
     7441 caccatgttc tcaatcccat tccttaacca ttggattatt gcaattgtac ttattattat
     7501 tttgggtatt ggattctcat tggttccctc ggcaatgtgg ccttcagttc caaaaattat
     7561 cccggagaat cagcttggaa ctgcttactc attaattttc tgggtacaaa actggggctt
     7621 aatgggtgtt cctgccctta ttggttgggt gctcgataag tactgtgtaa ccggtcaaaa
     7681 agtggttgaa ggcgtaacag ttaataccta caactacact ataccaatga taattttcac
     7741 ttcgcttgga atacttgccc tagtttttgc attactgctt aaggctgaag ataagaaaaa
     7801 gggttatgga cttgaactgc caaatattca aaagtaattt ttcattttta aagaggatgt
     7861 ccgcatcgcg gatgtcctct ttatttgcat ttgcctgttt attaactttt tcacaacaaa
     7921 gtttctctca agaagttttt ggtacacaaa tagagattga taggtttgca atggagatgc
     7981 aaaccgtaga tgagagcaca acacttattt caacaatagt ctcgtttaca gctattcaag
     8041 atcagatatc caatacacta tccattaact tcccaaaagc gcaggaagtt attataaata
     8101 gaagtaaagc aagaattacc gactcaaagg gtaatgttat ttggaaaggt agccttgaga
     8161 aaaaagttac atcggaaaac acgcttaact ttaagtttgg taaagactgg ctgaataaaa
     8221 aaggtgctca accatacacc attgagtggg aaacgaatgt tttggtaagt tcgcctaccg
     8281 gaatctatgt ttggcctgcc tgcctttcag gaaacattaa ctcagtaaag tacctcggat
     8341 tacgagtttt tgccgacaac gagtttagag aaaaaattga aaccaacctt gaggtaagct
     8401 caaggttaat ggatcaatcc tcaggtctta gcggtgtagt ttgggagttg agcaactatc
     8461 gtacaaaaaa ggcctggcaa tataccaagc ctattgaaaa tccctttgta aaaatatctt
     8521 tctaactgtt tttcattccc ttacaattgt ttcgtccctg tttggtccaa cagaaataat
     8581 cctaaccgga acgcctgttt cctgctcaat aaactggatg taagccatta gctcgtcagg
     8641 caaatcacta tcagatttga agcctgaaat atcggtcatc caacccttaa actccttgta
     8701 aactggctca atgggcgtat taatatcgta gggcaaccta tcggtttcaa taccgtttac
     8761 cttgtatgct accgcaacct tgatggtttc aaaggaatca agcacatcgg gtttagtcat
     8821 tattagctgg gttacaccat tgagcataat ggcatacttc aatgcaacta ggtcgagcca
     8881 accacaacga cgcggcctac cagtggtggc accaaactca acgcctatct ttcgcatctt
     8941 ttcgccaatc tcgttatcaa gctcggtggg gaatggtcca gagcctactc gggtacagta
     9001 tgccttgaat atgccataaa cctcacccac cgcatgggga gcaataccca accccgtgca
     9061 agcgccggca caaatggtgt tcgatgaggt tacgaacggg taagaaccaa aatcgatatc
     9121 gagcaatgat ccctgagcac cttccgcaag cacccgtttt ccctgtttga tgtagctatt
     9181 tatttcgtac tcagagtcga taaggtcgta ctgacgtaga agctctatgc ccttaaacca
     9241 ctcctcctca tgctgctcaa tgttgcttga atagttatat tgccttaata tttccttgtg
     9301 tttgttcttt aggaaattgt atctatcctt aaaatcgggt aagaggatat cgcccacacg
     9361 tagcccatta cgtccggttt tatccatgta ggtagggcca attcctttca gggttgaacc
     9421 tatctttcct tttccctttg ctgcctccga tgctgcatcg agctcacggt gcgagggtaa
     9481 aatgaggtga gcctttttgg aaattttgag cgattttttc aggtttaccc caagcttctc
     9541 aagcgagtgt atttcctcag ccaggataat tgggtcaatt accacaccat tgccaattaa
     9601 gtttaagcag ccaggatgga atacccctga aggaatggta tgcaacacat gtttaatgtt
     9661 attaaactcc aaggaatgac ctgcgtttgg accaccttgg aatcgtgcaa ttacactgta
     9721 attgggagtt agaacatcaa caactttccc tttaccttcg tcgccccact ggaggcctaa
     9781 tagaatatca actttactca tctcctttaa taattatgct gcttttggca aataaaatcc
     9841 ttagcgaaac caattgttca cacaaatttt aaggccaaaa agcatataaa ggtaatgctt
     9901 ttagcacaat tttgaaaagg aagatattaa caatctaaag ggaaagatca accttatcta
     9961 tttcggtttg acaatcttta caaaggccat aaaggtaaag cgagtgatgc gaaatagtga
    10021 atccactatt cttttgggcg gagttaatta tttcctgtat tcgggggtcg caaaactcaa
    10081 ttactttacc acagcgacta caaataaggt gatcgtgttg cttacattca taagctttct
    10141 cgtatagcga tgaattttta ccaaactggt gttttattac tagcccacac tccagtagca
    10201 agtcgatagt gttgtagagc gtcgccctac ttacctggta gtttttattt ttcattttga
    10261 tatagagcgt ttctatatca aagtgcgact cctgagagta aatctcgtca agaatggcga
    10321 aacgttcggg agttttcctg tgccctttac gctccaggta atcggtgagt atgcgtttta
    10381 ctacaagttt agtatcgctg caggtcatag cttaaattta gacttacttt aaatagcaaa
    10441 aataggataa aatttttgtt atacaacatc taacagtaaa acatagaaaa agtttatccg
    10501 aagtgccatg aattaaccgg gctcatttct tgaagaatga atcggaatga gacggaatag
    10561 gcggaagtta gaggaagttc tagtccgtta aacgtgaacc gttaagaacg cgtgactttg
    10621 agtcggaatg agtcggaata gatggaatat gatggagtta gaggaagtta gaactagtta
    10681 aaagttaaaa gttaaaagaa tagtttcacg aacaacgact taagccccgt aggggcgtaa
    10741 tatttgtaac accatgatta ttcccacatg tatagcgatg cacgcaatac attttccaaa
    10801 gagtaacaca gaaacgttgc atcgcaaaag aaaagtatgg taaatagcga tttccaaaaa
    10861 aacaaggatt aaaggtaagg agatgcttgg gttagctcag catgataagg atggttattc
    10921 gttgaaaact acttgtaata atgttgtcat gttgagcgct agcgaaacat ctcattgagg
    10981 tctattagag atccttcact ccgctgggga tgacaatggt agtagttgtt tgttaataat
    11041 aggccttgcc taaaatttta acattaatta tagctatcat tttgggcata tgcttacatt
    11101 tgtgaaaaat attttgagca tatgccaaga agcaagtgtc cacgaagaat ctgccattcc
    11161 cctgatattc atttattcaa gcctgcaggt aaacctttac acttgatgga aattgttgtt
    11221 ttagaagctg atgagcttga ggcaataagg cttgccgacg gtataggttt gtatcacgaa
    11281 gaagcggcaa aacaaatggg ggtatcaagg caaacctttg ggcggatact tgagcaggca
    11341 aggagtaagg tggcaaaagc gctaacaaac ggtatggctc ttagaatcaa caaaagccaa
    11401 actgaaatat tctgcgatca gcaaaataac cagaattcag aacacatttg atatagagaa
    11461 taagtggttt actaacaaaa aaaatgatta tgaaaattgc agtaccaacc agacatggcc
    11521 atgttgatga acattttggt cattgtgagt tctacacggt ttacaccatt gagaacggca
    11581 aagtaacagc aaaggaaaca cttggatcgc cccagggctg tggctgtaaa tcgaacattg
    11641 catttgattt acaacaaatg ggtgttgaaa taatgcttgc cggaggaata ggttcaggag
    11701 ctattaatgt gcttgcagct catggcattc aggtagttag gggttgctca ggaaataccg
    11761 atgaggttgt tgacctctac atcaacggag agttaacgga ctcaggagaa agctgtagcc
    11821 atcatgaaca tcatcatggc tcacaccaag gcgaacatgg tcactcgtgt aaccattaac
    11881 acgctttgaa taaactcatt gcggttgatt aatagaaata cctgaggaat aaacattaac
    11941 ccgggttgca cattgcaatg gtgtgtaatc cgggtttaat catcaaactt gtccattcgc
    12001 cgaacgttct ccattccctt aaccttcata atatttagta tcaggttgtt aaggtgagta
    12061 acatcatgca cgtataggtc aataaagccc tcaaaaatgc catcgtggct ttcgatatgg
    12121 aatttgcgaa tgttcaccat tagctcatcg gaaatcactt tggtaagctc actgataatc
    12181 ccgattcggt ccattccgtt aacctcaaca cgggcaagga acgatagggc tttctgggtg
    12241 gtccactttg cagcaacaat cttatcgcca tgttgagcca tgagcctaac agcctcagca
    12301 caggttgcct tgtgaataat tacaatatca tcgggcgaga ggtacccaat aacctcgtcg
    12361 cctggaattg ggttgcagca atttgctaca agatatgacg ggttgtcgga gtcagtgtcc
    12421 gcgcccagca ctatgggctt ttttaagtcg tactttattt ggatttcgct ttcgggctct
    12481 acctttttag cgccaaagcc tagctgcaac ttccagtact taatccactt gctttgggta
    12541 ttttttttca gaatcttttt caggttatcg aggttgataa gccctgaacc aattttggca
    12601 taaagctcat cctttgatga aacctcataa gctggtagaa ttttcttgaa tacacgcgat
    12661 gagggttgaa tacccatctc taaaagtttt tcctcaagca attgctgccc cttctctatc
    12721 ctattcttta cttcaccttt aagggcgttt ttaatggctg ctttagcttt ggccgtagta
    12781 gcaaaattga gcaattccca tgttggtttt tgtgaggctg cagtgattat ttctacctgg
    12841 tcgcccgtgt tgagctggta gcttaatgga accagcctat ggttaacctt ggcacctata
    12901 gctctattcc caacctcagt atgaatttcg taggcaaaat cgagggcggt tgctccctta
    12961 ggcaagctac gggtttcgcc ttttggagta aatacaataa tctcggaggt aaacaggttt
    13021 agcttaaact cgtcgaggaa ctcaagtgca ttttcttgtg gattttcaag catctcccta
    13081 acgcgtcgaa tccatttatc gagttcattt tcctggctgg tggcatccat ttccttgtac
    13141 cgccagtgcg cagcaaaacc tcgttctgca ttttcgtcca ttcggcgtga gcgaatttgg
    13201 acctcaaccc acttcccgtc aggtcccatc acggtacagt gcagggcttc gtacccgtta
    13261 gcctttgggg tgctaaccca atcgcgcaac ctgtcgggct ttggcttgta aatatcagta
    13321 atcagcgagt aaatgtgcca gcactgggtt ttttcaggta tatgtggata gggattaaaa
    13381 actatgcgga ctgcaagtaa atcgtatatc tcctcgaacg aaacgttctt ggtttgcatt
    13441 tttttccaaa cggagtaaac tgattttggt cttccactga tctcaaaatc gatgttattc
    13501 tcttcaagtt tctgaataat tggtaacgag aagtgattga taagttgcac ccttcgcttc
    13561 tcgttgtcgg caattttact ccttatctct tcgtatactt gagggtaacg atactttaaa
    13621 cttaggtcct caagttctgt tttaatggcg tagagtccta aacggtgagc caatggtgca
    13681 taaaggtaga tggtctcgct cgcaattttc atttgcttgg tagccggcag cgactcaaga
    13741 gttcgcatgt tatgtaacct atcggccagc ttaataagta ttacccgaat atcgtcggca
    13801 agggttaaaa gaattttcct gaaattctcg gcctgaatgg ttgagtttgc atcaaaaaca
    13861 tcggtaatct tggttaatcc atcgatgatg gaggcgattt tcttcccaaa aattctttcg
    13921 atatcctcaa tagtataatc ggtatcctca actacatcgt gcataagggc acaggcaaca
    13981 gcctttgcac ccagtccaat ctcgctggtt acaatttttg ctaccgcaat gggatgcagt
    14041 atatagggtt cgcccgattt tcggcgaacg cccttgtgcg cctcgttggc aagattaaaa
    14101 gccttaacaa ttagctcttt atcctcaggt gagttgcacc gggtacagct attaagcaaa
    14161 tcctcaaaat gcgattgtat aagaagtttt tcctcgtcgg tttgcaaact catgctattt
    14221 ccaattagct attttttggg gtaaaccacg gagatgcttc cggttttaat tatatcacca
    14281 gcatcggcag tactaaccac taaacggtaa acgtaagttc cctctttaac atactcacca
    14341 cggctattgc gtccgtccca accctgatta ccatggtaaa ccacaccgcc ccagcggtcg
    14401 tatatggtta agataaagtt agcttcaaaa tcgataaccg gcttgaaaag gctacgctca
    14461 tggccatttt cgcttcgggg atcattcggg gcaatggctg taggcatagt taccccgggt
    14521 agaatttctg tcgatatttc acgcgaccgg ctaagagtta ccgtatcgtt agcaggcgag
    14581 agctctaatg cctcaatgta gtagttgaac cttatccggt aaagtgggct ttgtccttta
    14641 aaccctgata aatcatcggc atattcggta accgacagcc cgttggcaat tcgctctaaa
    14701 ttgcctgggc cagaaatatc atcgttaacg gtaaaggaac gcctatagat ggagtactca
    14761 atcctattgc cagccctatc gggtttacgt tgggtatcga ggattaaagg atcccactgg
    14821 atactgacca cattgccctt ggggcttagg cgaggtatga tggtattgca gagatttgaa
    14881 accaaaacgg catcgttgca gccattatag gcatcaactt tatagtaaaa cgtgcgggtg
    14941 cgggctgccc atgtatcgtt ggtatcaatg ctcatgcttt tggtgggaac atcaaaccct
    15001 tcaatagttt ttgcgctgaa aatactctga gccgtatcgg gctgttccca tcgccagagt
    15061 ttgtaatcga taacgcggct attgggatcg atggtgtagt aaattctatt accattgttg
    15121 gttgctataa cgctatcgac actgacatag gttggcctgc gggccatttt ggtatggatc
    15181 aggtataggt tggagtaact ctcatagcct gtatccattc gggttgcttt gatgtaaaag
    15241 aaataatcct ggttttcctg aacatcccta acactatgcc tattgctata tccctcgtaa
    15301 tcgccaattt gagtataggc actccagttg aggtcgttcc ccatgtagag ctggtaacgg
    15361 atattgcggt aaggcgagga ggcattcaag ggccaagctt cataaccaac ccaagaaata
    15421 tcgattttgg cattacagct atcgtagctt gcggtaatcc aaacctgcga gtgtttaact
    15481 gtcatctgac tgggttcaga ggctccgagc aatgccactc ggtagtaaac cctttgtcca
    15541 ttcccattgg caccaatatg ggtgtactgg aatgtatttt ggtcaaccgt agcgatggaa
    15601 tcccagccgt cggcatcggg gtcattggga ttacggggga cgtaaataat ataacccaat
    15661 ggatcggcaa tttggggctc atggggtggt ttagtccaaa atatcgttac atccccatta
    15721 tccagattgt tagtgatata atcgagcgtt ggaggattag gcttattgct aaactgcgcc
    15781 caaagtgttg gcatataaaa ggttaagcca atcatcaaaa ttacttgtcg tatatttttc
    15841 atacacatta ctctttgtac cctctaattc ttttgcttcc ggcaatgcta actaccatcc
    15901 tgtcaacatc caagtatttt tttgccagcc caagaagttg tgtgggtgta atttcattga
    15961 ttgttctaat cagattattg atatactcaa aatcaaggtt gttatactgg aaaaggcttg
    16021 caaggctatc ggctatagca aatgggccat cgaaagttct aagtacttga ccggtcatgt
    16081 agcccctaac aagttccagt tcatcgttgc ttactggttc tgtctgcaat ttaatcatct
    16141 ctttaaatac ctcatctact gttttttcgg taaaggccga gccaacctcg gttgaaacaa
    16201 ccagcgccga cagttcagcc attggaataa tgaacgagga tattccgtat gtaaaccctt
    16261 tgtcttccct aatattgcgc atcaggcgag agccaaagta cccacccagt atggtattaa
    16321 gaacaactaa accgggcata tcggggtgat tgcgtttggg taaaaccctg cctattcgca
    16381 aagctgcttg aactgcatct tttttatacg aaaaaactct gtaatcggtt gtggtaaatg
    16441 agggtaaccc taactcaatg tcatcgcatt gttcaaaaat ggggttgttg aaaccattta
    16501 ctagaaaatc gacctgttta gcatcaacct ttccagcaat aacaatcaaa ccattagagg
    16561 ctttatgata acgattacgg aactgaacta aaacggtttg tgatagcaca tcgtaatcgt
    16621 taggggtagc aaaagagcca tagggatggt tgctaccgaa aagagcactg aaaaatcgtt
    16681 ggcgtgagag ggtagatacc ttttccaaat cgaccaataa cgattgtttc ccctttttgc
    16741 agaaaagttc cagctcatgt tgcgggtaag taggattagt taaaatatca gccaaaacat
    16801 ccaatgagcg gtagaagtgt ttaccaatgg tgtaaaaggt taccgatgca aaatcgcgtg
    16861 agaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttataa atggcgttga
    16981 aaggaacttc gcgctccagg gtaaggaaac ggggttcggt ttgctcaggt tcccttggaa
    17041 tgcttttatt tgcaagttta cgtggttcaa tggaaccaaa ccatttatgg gtaagctcca
    17101 gaatttcatt ggggtcaaca gggcctgcca cacaaagtat tgcgttgttt ggggcataat
    17161 ggctaaagaa gaaatttttt acatcggcta atgtggcacg gcttatatgc tcgggcgact
    17221 gtccgatggt tggccaacgg tatgggtgaa ccttgtaggc caaaggccga agatacaacc
    17281 aaatatcacc gtaaggttgg tttaggtatc gttggttgaa ctcttccagc actacacttt
    17341 tttgtatatc gaggcttttc tgcgaaaagg caaggctaag cattctgtcg ctttcaagcc
    17401 aaaaggctgt ttcgatgttt tcagtaggaa cggtgatata gtaattagtt aaatcgttgg
    17461 aggtaaatgc attattctcc ccccccaccc tctcaagcgg ttcatcgaat acaggtatgt
    17521 tggccgaacc accaaacatt aaatgctcaa acaggtgggc aaagcctgtt cgttccgggt
    17581 cctcgttttt tgaacccacg ctgtaaagca ggttaaaggc tgcaatggga gtactatagt
    17641 cagtgtgaac tattagcact aaaccattat caagcttata tttcacaaaa tcgatcatac
    17701 tcacaaaaat atcctcaata ttaactattt ttgttgatgt tttaggtata atgattagag
    17761 atttaaaagc aatgtcgata ttttgaggca ttgctaaact tctttttaaa ctagcaacat
    17821 gctaagggag gggtttaaaa tatcgctaac aaagttctgg gcaatttttc gcagcttagg
    17881 agagcgaaga atcacactga ttttaagttt tattattgga cttttaagcg gtgttgccgg
    17941 tgttgcatta aaaaatacgc ttcacggggt taggaatttt gtcaacggat tggtgcccat
    18001 ggacaccgca agcttgcttt actttggttt gcctgcaata ggaatcttac taaccctact
    18061 atttgttcgc tacctcatta aagacgattt aagccacgga gtaaccaagg ttttgtattc
    18121 catttcgcga cagggtagca agctaaaggc acaccatatg tttagttcca tttttggcag
    18181 tgcccttacc attgggtttg gaggttcagt gggagctgag gctccagtag tgctaacagg
    18241 gtcagcaatt ggttcaaacg ttgggagctt tttcaggatg aattacaaaa tccgaacact
    18301 gctaattgga tgcggcgcag caggtgccat agcatcaatt tttaaggcac ccattgctgg
    18361 cattgtgttc acccttgaag tactaatgct cgaccttacc accgcttcca ttgtgccgct
    18421 gctaatttcg gccgttacgg catcgtcggt tagctatttg ctgatggggc ggaatgtggt
    18481 gtttgcctat gatgttgttc ggccatttgc actccacaat atcccctggt ttatactgct
    18541 tggtatattc tgcggtttgg tgtcacttta ctttatcaga tcaatttgga gtatagagaa
    18601 acgctttaag cagacacgga gcccatacaa gaaatggata gtgggaacta taattcttgg
    18661 cgcgttaatc tttgtttttc caccgctttt tggcgaagga tatgatgttc ttcaggcctt
    18721 gctcaatggc cagtcggagt atatctttca taacagcccg ctgtatgggt ttcagaataa
    18781 ctactggctg ctaattggct cactctttct gatcattgcc cttaaagcgg ttgccactgc
    18841 cgtaaccaat ggagctggag gggttggagg aatttttgcg ccatcccttt ttattggtgg
    18901 catttcgggt tttttggtag caaagctaat taaccagttc aactttattg atgtgtcgga
    18961 aagtaacttt gccttggtgg gaatggctgg aactatggcc ggggttatgc atgccccatt
    19021 catggctata ttcctaattg cagagattac tgggggctac gctctatttg taccccttat
    19081 gataacctca accattgcat acctcaccat tatgtacttt gaaccccact ccatttacac
    19141 taaacggcta gccacgcggg gcgaacttat tacccaccac aaggataaag cagtactcac
    19201 cctactaaac gtaagtaagg ttattgaaac cgattttata cccgttgaac caacggaaac
    19261 ccttggttcg cttgttaaaa aggtaagcca atcgcgccgg aacgtttttc ctgtggtaac
    19321 caccgaaggt aagtttttgg gtatggtaat gctcgacgac atcaggccaa tcatgttcga
    19381 tgcctcacgc tacgatacca caaccgt
//
LOCUS       DAOOWJ010000046        19220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_2858_length_19220_cov_7.131698, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000046 DAOOWJ010000000
VERSION     DAOOWJ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 19220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19220
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2858_length_19220_cov_7.131698"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /gene="groL"
                     /locus_tag="PLW22_06165"
     CDS             complement(<1..1093)
                     /gene="groL"
                     /locus_tag="PLW22_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOU74324.1"
                     /translation="MSSKVLTFDIEARDRLKKGVDQLANAVKVTLGPKGRNVVIEKKF
                     GSQQVTKDGVTVAKEIELPDPLENVGAQMVKEVASKTADNAGDGTTTATVLAQAIVNV
                     GLKNVTAGANPMDLKRGIDKAVAKVVEHLKKQSVSVGDDYLKIEQVATISANNDNEIG
                     KLIAEAMKKVKKEGVITVEEAKGIETTVEVVEGMQFDRGYISPYFITDPEKMEAVLEN
                     PLILITDRKVSTMKDLLPILEPVAQNGRSLLIIAEDVEGEALATLVVNKLRGSLKIAA
                     VKAPGFGDRRKEMLEDIAILTGGVVISEEKGLRLDSAKMDMLGRAEKVTIDKENTTIV
                     NGGGKKEAIDKRVAQIRTQIENTTSDYDRE"
     gene            complement(1128..1406)
                     /locus_tag="PLW22_06170"
     CDS             complement(1128..1406)
                     /locus_tag="PLW22_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOU74325.1"
                     /translation="MSNVKVKPLADRVLVEPMEAEEKTSSGIYIPDTAKEKPQKGTII
                     AVGPGTKDVNMEVKVGDVVLYGKYAGTEITIDGKDYLIMKQSDILAVI"
     gene            complement(1979..3352)
                     /locus_tag="PLW22_06175"
     CDS             complement(1979..3352)
                     /locus_tag="PLW22_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOU74326.1"
                     /translation="MKRILVLPLVTAIMLGCGGSKKVAQAPITPSWVQGRPTQAGYYI
                     GIGSARKTTPDYQQAAKQSALADLASDISVSISSQSVLNAFESQNYFSEDFRSSIRAE
                     AQKELEGYEVVDSWEDQNTYWIYYRLSKDTYRRMVEEKKAAAAAKSLDFYDKAITAID
                     GNDSRTALLMLVKALEPIKPFFAETITATYRDKQIFLGNEIISTLTSTLNSLTLNGPK
                     AVNVKLGFGIESNQLTYLVTSSNGMPQKSIPLKISYTERPLTNNRIITNNQGEASFSI
                     DAVRSRKLSETISVEVDFESIVKEATRDFAIGKILARFKTPSAETQVNVIRPKFLVSS
                     TETNLGAAIERKPLAESLKRKILEAGFPITENPAEADYRINIKSSTRQTGQSGQYIQV
                     SLNLQVTVTNASGNTVYSHSTDRINASHFEAQTAGINAYTNAAKRIESNIVEELIERI
                     IKGDRAY"
     gene            complement(3357..>4286)
                     /locus_tag="PLW22_06180"
     CDS             complement(3357..>4286)
                     /locus_tag="PLW22_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74327.1"
                     /translation="EVKRLQPDYGNTQDMLKVSKCEPLYRQAKQFMDAAQYRKAYANL
                     DKIVKEFTNYKDSYDLRYEALQKATVTIRVTDFKPTTYNAESIAKSVQAEVVSKLNAL
                     NNPFVKVIDVQSTDKIIEEQQRSVTTGSDLEIGKLLAAKALLSGEVSVIEVTQGKLNK
                     TEKRGYIREESKTKDPKTGEEKVNVTYRKVIYWVYNQKNSLSVALKFQLASTETGAVM
                     VSDFIRSNQADEVNYATFDGPSSKLVPGYWEKINADSPKDVINDNQLDVRNLQELLKA
                     KRDIASMNTLMSNAIEDIAKRTAQKINQYNPEE"
     assembly_gap    4287..4386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4776..5414
                     /locus_tag="PLW22_06185"
     CDS             4776..5414
                     /locus_tag="PLW22_06185"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOU74328.1"
                     /translation="MSSSAARIVKGIELNLGKPLPGYDAQKRMEPSVRADLLGNTTPN
                     LSTRKSAVMILLYPSGQGLSFVLIKRQTYDGPHSGQVSLPGGKVDESDASIEGTALRE
                     TYEEVGVEPNQITILGQLTELFIPVSNLLVQPFVGIVAQKPEFSPNLQEVEYIVEVDV
                     AHLLDDSKKSVKVITSHGRPITAPYYSFQNEMVWGATAMILSEFEELLRRSL"
     gene            complement(5533..6993)
                     /gene="ftsZ"
                     /locus_tag="PLW22_06190"
     CDS             complement(5533..6993)
                     /gene="ftsZ"
                     /locus_tag="PLW22_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU74329.1"
                     /translation="MGDDIINFDLPIINQSIIKVIGVGGGGGNAVNYMHRMGIKDVDF
                     VLCNTDAQALVNSPVEFKVQLGATLTEGRGAGNKPEIGRQAAIESLQQIVSLLQKNTK
                     MVFITAGMGGGTGTGAAPIIAKAAKELGILTVAIVTIPFRTEGRKRMMQAIEGIRNIS
                     QHVDSLLVINNEKLLEVYGNLKLTEAFAKADDVLATAAKGIAEIITVHGYINVDFADV
                     ETVMYNSGVALMGSGRANGQNRALEAVKQALESPLLNNNDISGARNILLNITYGQDEI
                     TMEEVGVIIDYVQHAAGDDADLIWGNGCDPSLDEDLKVTVIATGFGTNSIPELYVKER
                     NIEVISLDSEQHATKVELVNDTPEIDPIPARQTSKPKKQPVIDPAQRVIEFDVNSDVF
                     EVKTVQAAKANEIEIDIQPASPKPATPQAAPFAGTSNNKWANLDEIENVPAFKRRNMN
                     IEPNEPPKTTTISRVTLDDDDIIINPENPYLYDNVD"
     gene            complement(7057..8391)
                     /gene="ftsA"
                     /locus_tag="PLW22_06195"
     CDS             complement(7057..8391)
                     /gene="ftsA"
                     /locus_tag="PLW22_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOU74330.1"
                     /translation="MAQDNNTYVAAIDLGTTKVVTLIGKRTTNGRLQIVGFSSTDSTG
                     IKKGMIQNIEETVKAVEKTVREAESQAGVKIKSAFVGIAGQHIYSIKNYGYITLGSEE
                     TEIKKSHIKRLIEDMYNIPVDTGDSILHVLPLDFSIDNVKIEDDKPIGMLGRRLGANF
                     HIVIGKTSSAKHIEKCVDRAGVKVSELILEPLASSKAVLTDEEREAGVALIDIGGGTT
                     DLAVYYNGIVRHTAVIPIGGSIITQDIKEGCQILQKHAEMLKLHHGYALMSMAPDNKI
                     ITVPGIGGREPKEISMRSLAGIIQARMEEILEYAMYEIQMSGFANKLTAGVVITGGGA
                     LLKHIVSLIKFNWGYDVRIGYPSEMLCSESLENYNYPQYSTVIGLGLMGLEYIDAGEA
                     SPCTTTETKIEENKTEEFAEVQAKESKQINKRTLGMKITDVLSKLFAEDDIN"
     gene            complement(8397..9203)
                     /locus_tag="PLW22_06200"
     CDS             complement(8397..9203)
                     /locus_tag="PLW22_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74331.1"
                     /translation="MNRKLRIGLKVLAWLFVLAWVIYGLSYANKVYKGKRCTKLEVRV
                     LDSAKLNFVRAKDVYRTIVKSKNSPIGQSLNSINTNRIEQDIYQMAAVRDVQAFKTAK
                     GEVIVEITQRQPVLRVFNAKGQTYYIDDSGKILPYSSGFAANTLVANGNIVEPFKVVP
                     NLDVTAFTDSSKEGPKNFIHKLYEFAMYVNNDKFWNAQIEQVYVRNRNNIELIPLVGP
                     HIIVLGNLDNYEVKLKKLKLFYEKALPAEGWNKYTVINLKFKNQIVCTKY"
     gene            complement(9196..10593)
                     /gene="murC"
                     /locus_tag="PLW22_06205"
     CDS             complement(9196..10593)
                     /gene="murC"
                     /locus_tag="PLW22_06205"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOU74332.1"
                     /translation="MELNAVKRVYLLGIGGIGMSAIARYYLHYGVPVAGYDRVSSDIT
                     QNLEAAGANIHYTDSVGAIPRAFLNREETLVIYTPAIPANHSELEYFRSNRFVLMKRA
                     QVLGLLSSNYKTLAVAGTHGKTTTSTLLAHLLATSMGCDAFLGGISRNYSSNLILADQ
                     GKQLLVVEADEYDRSFLTLHPHLAIVTSVDADHLDIYGTYDAVLNAFQEFVSQIKPGG
                     YLVVKQNIAFKPIVGKETEVITYGFTPDCHYYPTNISIHDGLYRFTLVTPQGSINNLT
                     LGVPGHYNLENAIAASAAALTLGVPTEKLAHGLESFRGVQRRFDVRYRGTKIIYIDDY
                     AHHPSEIEALVRSVREVFPNRKITGIFQPHLYSRTRDFAAEFAAALDKLDKPIITEIY
                     PARELPIEGVTSETIMKLMSNSNAQVVPKAELISWLKCNNVDILLTIGAGDIDRMINE
                     IVNILEAKENRAINE"
     gene            complement(10598..11725)
                     /gene="murG"
                     /locus_tag="PLW22_06210"
     CDS             complement(10598..11725)
                     /gene="murG"
                     /locus_tag="PLW22_06210"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOU74333.1"
                     /translation="MEKNRLRIIISGGGTGGHIFPAISIANAIREQKPDAEILFVGAE
                     GRMEMDKVPQAGYNIVGLPVAGFSRSLTPKNLIVIIKLIKSLVKANRIVKHFKPHVVV
                     GVGGYASGPIVKAAQRKGIPTLLQEQNSYAGVTNKLLAKKAKTICVAYEGMEKYFPAQ
                     KIKLTGNPVRQDIENLSVTRNQGLEHFGIGADKKVLLVLGGSLGARTINQSLMASIHL
                     IKARHDIVVIWQTGKLYYQTVKEALDSNPVENIRLYDFIARMDLAYVSANTIISRAGA
                     GTISELCLVAKPCILIPSPNVAEDHQTRNAQALVSRDAAIMIPDSQAKEQLVKAAFEL
                     LDNPAEQKKLSENIARLALRNSAITIALEVLKLAGIKKTKN"
     gene            complement(11694..12863)
                     /locus_tag="PLW22_06215"
     CDS             complement(11694..12863)
                     /locus_tag="PLW22_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU74334.1"
                     /translation="MGALFDKYLKGDRVIWMIVIFLYLSSLLLIYSSSSSLAFRYHGG
                     NTTYFLIKQLVFLLVAFIVMWTAHLIKYSVWYRLANILLALSVPLLGITLLFGRNLNA
                     ASRWLEIPGLGISFQSSDLAKVILIMYVAKYLSQMQENTDDFKQFLIQLIAPVAFVCA
                     LILPANFSTAALLGLTCWLMMFVGRVNLKYLFGITGIGIVLVGAFIAISLQFKTLSRA
                     QTWVNRIENFISDDKTEGNYQLEQSKIAIATGGIIGKGPGRSTQRNFLPHPYSDFIYA
                     IIIEEYGVVGGVFTLILYLMLLYRAALLVKKSNRLFPAFLAFGLALMLTLQALINMAV
                     AVGAIPVTGQPLPLVSLGGSSLIVSSGALGIILGISRSQDKKELFDGEEPVKDNN"
     gene            complement(12880..14214)
                     /gene="murD"
                     /locus_tag="PLW22_06220"
     CDS             complement(12880..14214)
                     /gene="murD"
                     /locus_tag="PLW22_06220"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOU74335.1"
                     /translation="MKRIVVLGGGESGAGAAVLAQKKGFDVFLSDMSEIKPRYKEWLN
                     KYDIRFEEGRHTEELILNADEVIKSPGIPDKAPMVVKIREKGIPIISEIEFAGRYTNA
                     YTVCITGSNGKTTTTSLIYHMMQKAGLNVGLAGNIGNSFAYQVAECSYDYYVIELSSF
                     QLDGIYSFKPDIAILLNITPDHLDRYDYKMQNYVESKFRITRNLSEDDCFIFCSDDAV
                     TVEQLKKIVLRAQQLPFSQKTKENQTAWLENLDMFINYDNSDFSMSINDLSLKGKHNI
                     YNSMAAGIAGHVLNIRKEVIRESLSDFQGVEHRLEYILTVRGVKYINDSKATNVNSAW
                     YALESMTTPVVWIAGGTDKGNDYSELMDLVKQKVKALICLGVDNSKLHAAFDGVVPVI
                     VDAKSAEEAVQHAYRISVPGDTVLLSPACASFDLFENYEDRGRKFKAAVRAL"
     gene            complement(14226..15479)
                     /gene="mraY"
                     /locus_tag="PLW22_06225"
     CDS             complement(14226..15479)
                     /gene="mraY"
                     /locus_tag="PLW22_06225"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU74336.1"
                     /translation="MIYYLAQYISKFWDFPGSGLFRYISFRSAMAFIVSMAFALLIGK
                     RIILYLQRKQIGEEIRNLGLEGQMQKKGTPTMGGIIILLSMLLPVLLFADILNIYIIV
                     MLVTTVWLGAVGFADDYIKVFLKNKEGLSGRTKIIGQVSLGLFVGLVMWLSPEIVVRE
                     KVERVKKDVQIETVVTSEYNDNQPVTVSKAKKTTQTTIPFVKNNEFDYNLLNPFKGEG
                     YEYVTWIIFILVVILIITAVSNGANLTDGLDGLATGVSAVITTTLGVFAYLSGNVIYA
                     EYLNIMYIPYSGELVVFMSAFIGALVGFLWYNSYPAQVFMGDTGSLTIGGIIAVFAIL
                     IRKELLIPILCGIFLVENLSVVMQVSYFKYTKRKYGEGRRIFLMSPLHHHYQKKGYPE
                     PKIVTRFWIVAILLAVITVVTLKIR"
     gene            complement(15476..16939)
                     /locus_tag="PLW22_06230"
     CDS             complement(15476..16939)
                     /locus_tag="PLW22_06230"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOU74337.1"
                     /translation="MAKLQNIIPVKLITKTVGNINVDIDELTFDSRKVKVGCCFFAIR
                     GTQSDGHSYIPQAITSGATAIVCEELPAECPANVTFILVDNSSVALGYMASAFNGNPS
                     QKLKLVGITGTNGKTTTVTLLYRLARRMGHKAGLLSTVVNYVDNVEVPSTHTTPDPIQ
                     LNALLRQMVDAGCEYCFMEVSSHSVVQNRIAGLTFAGGIFSNITHDHLDYHKTFAEYL
                     KAKKRFFDELPSDAFALTNTDDRNGMVMVQNTKAKVYTYSLRSMADFRCKVIESHFDG
                     MMLNMDGVEVWTRLLGRFNAYNLLAIYATLMLLGFDKNDILTGISDVTPVSGRFEYVR
                     SSHGVTAVVDYAHTPDALENVIKTINEIKTENQRLITVVGAGGNRDKTKRPVMAKVAT
                     ELSDIVILTSDNPRFEEPEEIINDMRIGVDASQAKKVITIVDRREAIRTACMLALKGD
                     IILVAGKGHENYQEIKGVKHHFDDKEVLTEIFKSLQV"
     gene            complement(16941..19064)
                     /locus_tag="PLW22_06235"
     CDS             complement(16941..19064)
                     /locus_tag="PLW22_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOU74338.1"
                     /translation="MTVKNNILIRIAGIYILLLLVGIGIVFKIVFIQVVDGHELREKA
                     KKITYRDILVEANRGDILAADGRVLATSVPYFDLRMDLLAAGLTDSVFKANIDSLAIC
                     LSQFFGDRSWYSYRSELTNARKYAKNRRYYPIAPRKVNYLELQEISKFPLLRLGENKG
                     GFIAEQVNKRMLPHNSMAARTLGMVNLDGGVVGIEKALDPVLRGRNGLRVHARIPGNT
                     WVEVNSMNKVEPEDGVDILTTLDVQLQDVAEAALRKHLEGHGAGHGCAIVMEVATGDI
                     KAIANLRRNEDGTYEEVYNYAVGESTEPGSTIKTATLIALLEEGDINLNDTVNTGKGK
                     LQLYDHVISDSKEDGHGKITVKEVFEVSSNVGVIKLVQKVFKGKEKDFVKKLYELKLN
                     EPTGIIIPGEVKPQIRFPGDKGWSGLSMPMMSIGYEIRLTPLQILTFYNAIANNGRMV
                     KPRLVKAYLKHGQVVESFPPQVIQSSICSRSTLKKVHEVLEGVVESGTATNLKNPRYK
                     IAGKTGTAQIAKGRKGYKSGGRVSYQASFVGYFPAEDPKYSCIVVVNSPSNSVYYGNV
                     VAGPIFKEISDKVFATSPEWFSDIKDDELKDLPQTKTGQLSKLEYIMNELDIPFESNG
                     KKTDWVTVQRDSTKIATTPIRLAKSRVPNVVGMGLRDAIYQLERLGLRVVAKGRGTVR
                     SQSVNPGEPVIKGSTVYLEMSRGES"
     gene            complement(19084..>19220)
                     /locus_tag="PLW22_06240"
     CDS             complement(19084..>19220)
                     /locus_tag="PLW22_06240"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOU74339.1"
                     /translation="NLRAESITTAAQYMSISRQSEVVKLVEEKGLGLIESRVPPKKLK
                     "
BASE COUNT         4796 a         4387 c         4259 g         5678 t
ORIGIN      
        1 tttcgcggtc ataatcggag gtggtgtttt caatctgggt gcggatttga gccacacgct
       61 tatcgatggc ttctttcttt ccgccaccgt taacaatggt ggtgttctcc ttgtcaatgg
      121 taaccttttc ggcacggcca agcatatcca tcttagcgct atcgaggcgt aagccttttt
      181 cctcgctaat taccacacca ccggtgagga tggcaatatc ctcaagcatt tccttgcgac
      241 gatcgccaaa gcctggggcc ttaacggcgg caatctttag cgagccacgt agtttgttaa
      301 caacaagggt agcaagggct tcgccttcaa catcctcggc aatgatgagc aacgaacgac
      361 cgttctgagc aactggttcc agaataggaa gcaaatcctt catggttgaa accttgcggt
      421 cagtaatcag gatgagggga ttttccagaa cggcttccat cttttcaggg tcggtgatga
      481 agtatggcga gatgtatccg cggtcgaact gcataccctc aacaacctca acggttgttt
      541 caataccttt agcttcctca acggtgataa ctccttcctt cttcaccttc ttcattgcct
      601 cggcaatgag tttacctatc tcgttatcgt tgttagccga aatggtagca acctgttcaa
      661 tcttcaggta gtcgtcgcca accgataccg attgcttttt gaggtgttca actactttgg
      721 ctacagcctt atcgataccg cgctttaggt ccatagggtt agcaccagcg gtaacgtttt
      781 tcaaaccaac gttaacaatg gcttgtgcca gaacggtagc agtggtagta ccatcaccag
      841 cattgtcggc ggttttggag gcaacctcct taaccatttg tgccccaacg ttttcaagag
      901 gatcggggag ctcaatttcc ttggcgacgg ttacaccgtc cttggttacc tgctgcgaac
      961 caaacttctt ctcaataacc acgttgcgac ctttggggcc aagggtaacc tttacggcat
     1021 ttgccagctg gtcaacaccc tttttaagcc tgtcgcgtgc ttcgatgtcg aaggttaata
     1081 cttttgatga catagtctat cgtttttaat tgtttacaat caatgattta gataacagcc
     1141 aaaatgtcgg attgtttcat gatgaggtaa tccttaccat cgatggtgat ttctgtacct
     1201 gcatacttgc catagagtac aacatcgcca acctttacct ccatgttaac atccttggtg
     1261 ccgggaccaa ctgcaatgat ggttcctttt tgtggttttt cctttgcggt gtcgggaatg
     1321 tagattccgc tcgaggtctt ttcttcggcc tccatgggct ctactagcac tctatcggct
     1381 agaggtttta cctttacgtt cgacatagct tcttatgttt tttaggttta caactagttt
     1441 tccaaaattt cacgttaagg ttatttacac atcgtgtgcc aaaccttaaa actgactttt
     1501 ttgcatgaaa aaactgacag aattatgtca gaggagagtt gaaagtggca gcaatggcag
     1561 gaattgaagt tgatagttga tagttgatag ttgatagttt ttggttggca gctgttctaa
     1621 gtaccttgtc atgtttagcg ctagcggatc atcccattga ggtctattac agatccttcg
     1681 ctgcgctcag gatgacaagt gtagttgatg gttgattgtt gttagttgat ggtgagggaa
     1741 atgggttgga atgaaccgta atgaaccgaa atgaacggaa tgattcggaa tggtaaaatg
     1801 agcgattaga gtgcattaaa ccgaaatgcg tatcctgaaa tatttttaca ggaaatttgt
     1861 cagcagaaac aatttgttaa gatgtttttt gtgtttcaaa tgaccaatag cggtttttaa
     1921 agcaagctga acacaaaaaa ccaaacacta ctaaacacta tacactataa actgaacact
     1981 aataagctct atcgcctttg ataattctct caataagttc ctcaactatg ttgctctcaa
     2041 tgcgctttgc tgcattggta taagcattta tgcctgccgt ttgtgcctca aaatgcgaag
     2101 catttatacg gtcggtactg tgagagtaaa cggtattgcc gcttgcattg gttacagtaa
     2161 cttgtaggtt cagggaaacc tggatatact gaccgctttg gccagtttgc ctggttgatg
     2221 attttatgtt gatcctgtaa tccgcttcgg ctgggttttc ggttatggga aagcctgctt
     2281 caaggatttt ccgtttaagc gattcggcaa gtggcttacg ttctatagct gcacctaggt
     2341 tggtttctgt ggatgacaca aggaattttg gcctgattac gttgacctgc gtttcggccg
     2401 atggtgtttt aaaccgggca agaattttgc ctatggcaaa atcgcgagtg gcttccttta
     2461 caatcgattc aaaatcaact tctaccgaaa tggtttcgct tagcttgcgg gagcgaacag
     2521 catcaatgct aaacgatgct tcaccctgat tattggtgat aatcctgttg ttggtaagtg
     2581 gacgttcagt gtagctgatt tttagcggaa ttgatttttg tggcatgccg ttactactgg
     2641 taacaaggta cgtgagctgg ttgctttcaa tcccaaagcc tagcttaacg ttaacagctt
     2701 taggcccatt tagtgttagc gaatttaatg tacttgttag ggtagaaata atctcgttac
     2761 caagaaagat ttgcttatcg cgatatgttg ccgtaatggt ttcggcaaaa aatggcttaa
     2821 tgggttccaa tgccttaacg agcattagca gtgcagttcg ggaatcgtta ccatcaatgg
     2881 ctgtaatggc tttatcgtag aagtcgagcg atttggccgc tgctgctgcc tttttctcct
     2941 caaccattcg cctgtaggta tcctttgata ggcgatagta aatccagtag gtgttttggt
     3001 cttcccagct atcaaccacc tcgtaacctt caagttcctt ttgggcttcg gccctaattg
     3061 atgaccggaa atcctcggaa aagtagtttt ggctttcaaa ggcatttagc accgattgtg
     3121 acgaaatgga aaccgaaata tcgctagcca ggtcggcaag agcgctctgc ttggcagcct
     3181 gctggtagtc gggggttgtt tttcgggcgg agcctattcc aatgtagtaa ccggcctgag
     3241 tgggacgtcc ctgaacccac gatggggtga taggtgcttg tgcaactttt ttactccctc
     3301 cgcatcccag cattatagct gttactagcg gtaataccag tattcgtttc atcgttttac
     3361 tcttcggggt tatactgatt gattttttgt gcggttcgct ttgctatatc ctcaatggcg
     3421 tttgacatga gtgtattcat gcttgctata tcgcgtttgg cttttagtaa ttcctgtaga
     3481 tttcgcacat cgagttggtt gtcgttaatt acatccttgg gcgagtcggc attaattttt
     3541 tcccagtagc cgggaaccag cttggatgat gggccatcga atgtggcata gttaacttca
     3601 tcggcttggt tgctccggat gaaatccgat accattactg ctcccgtttc ggttgaagca
     3661 agttggaact ttagggcaac cgataagcta ttcttttggt tgtaaaccca gtaaataacc
     3721 ttgcggtaag taacattcac cttttcctcg cctgttttag gatccttggt tttactttct
     3781 tcgcggatat atccccgttt ttcggtttta tttaatttgc cttgtgtaac ttcaataacc
     3841 gaaacctcgc cgcttagcag ggcttttgca gcaagtaatt tacctatctc aaggtcggaa
     3901 cctgtagtta cacttcgttg ctgttcctca attattttat cggtactctg aacatcaata
     3961 accttaacga atgggttatt tagagcgttt aacttgctta caacttcggc ctgaaccgat
     4021 ttggcaatgc tttcagcatt gtaggttgta ggtttaaagt cggtaaccct tatggtaacg
     4081 gttgcctttt gcagggcctc atagcgtaaa tcgtatgaat ccttatagtt ggtgaattct
     4141 tttactattt tatctaaatt ggcataggcc ttacggtact gggcggcatc catgaactgc
     4201 ttggcttgcc tgtagagtgg ttcgcatttg gaaaccttta gcatatcctg cgtatttcca
     4261 taatcgggct gtaggcgttt aacctcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnngcat caatattatc gcgcttggcg gtaagcgaac ggatgtaagc atcggcggct
     4441 tgctcccaca aaccggcctg ctcaaacttc attccctttt tggcatactt ttttgaggca
     4501 catccgccac caaccagaac agttgctgct agaagtagga gtaaaagtcg tttcataggc
     4561 taatattgct tttggttaat attctttaag aaacatgata tcaatgggtt aacattaaaa
     4621 tcaatttaag ttaatttaaa tactttcatt caacccttaa atatagacaa atttggcaaa
     4681 ccttttttct ggttttgctt taatatcatt ttttgtgatg taagtaatca aaaagtaagc
     4741 caaaaagcta attttgaaaa acaataccaa ctggtgtgag cagttcagcg gcaagaattg
     4801 ttaaaggaat agaattaaac ctgggaaaac ctttgcccgg atatgatgcg cagaagcgaa
     4861 tggagccatc ggttcgtgcc gatttgcttg ggaatactac acccaacctg tcaacacgga
     4921 aaagcgctgt aatgatactg ctttacccct ccggccaagg gttgagtttt gttctgatta
     4981 agcgccaaac ctacgatggt ccccatagcg gacaggtaag cctccccggt ggtaaggtcg
     5041 acgagagcga tgccagcatt gagggaaccg cactgaggga aacctatgaa gaggttggag
     5101 ttgagcccaa tcaaattact attcttggtc agttaacgga actatttatt cctgtcagta
     5161 atcttttggt tcaaccattt gtgggcatag ttgctcagaa accagaattt tcacccaacc
     5221 tgcaggaggt tgagtatata gttgaggttg atgtagccca tttgctggat gactcaaaaa
     5281 aatcagtaaa ggtgataacc tcccatggcc gcccaataac cgcaccctat tatagctttc
     5341 agaacgagat ggtttggggt gccaccgcca tgattctctc ggagtttgag gagttgctga
     5401 ggaggagcct ttaaaaaggc attgtaacta ttaacttacg cattccacaa attttatgga
     5461 tcaagcatta atcaaaaaaa gcgcagccct ttggctacgc taagcaatca attccatatt
     5521 tgcaaattac aatcaatcca cattgtcgta caggtagggg ttttcagggt taattatgat
     5581 gtcgtcgtcg tcaagggtaa ccctggagat ggttgttgtt tttggaggct cgtttggctc
     5641 aatgttcatg ttgcggcgct taaaggctgg aacgttctca atttcatcga ggttagccca
     5701 cttattattg ctagttccgg caaatggtgc tgcctgggga gtagcaggtt ttggcgaggc
     5761 tggttgaatg tctatttcaa tttcgttagc ttttgcggcc tgcactgttt ttacctcaaa
     5821 aacatctgaa ttaacatcga attcaattac cctttgggca ggatctatta caggttgttt
     5881 tttgggcttg ctagtttgcc tggcagggat agggtcaatt tcaggtgtgt cgttaaccag
     5941 ctcaacctta gtggcatgct gttcggagtc gagcgaaatt acctcaatat tgcgctcctt
     6001 tacatagagt tctggtatgc tgttggtacc aaagcctgtg gcaatcaccg taacctttag
     6061 atcctcatca agcgatgggt cgcagccatt accccagatt aggtcggcat cgtcgcctgc
     6121 ggcgtgctga acgtaatcaa tgattactcc aacctcttcc atggtaatct catcctgccc
     6181 ataggtgatg tttagcagta tgttgcgcgc tcccgatatg tcgttgttgt tgagcagggg
     6241 cgattcaagg gcttgtttaa ccgcttcgag cgcgcggttt tggccattag ccctgcctga
     6301 gcccatgagg gcaaccccac tattatacat cacggtctcc acatcggcaa aatcaacatt
     6361 gatatagccg tgaacggtaa taatttcggc aatacccttg gcggcggttg ccagaacatc
     6421 gtcggctttg gcaaatgcct cagttagttt caggttgccg tacacctcaa gcaatttctc
     6481 gttgtttata accagcagcg agtccacatg ctggctaata ttcctaatac cctcaatggc
     6541 ttgcatcatc cttttgcgcc cttccgtgcg gaaaggaatg gtaacaatgg ctacggttag
     6601 tatgccaagc tcctttgccg cctttgcaat aattggtgcg gcacctgttc cggtaccacc
     6661 gcccatccct gctgtaataa aaaccatctt ggtatttttc tgcagcaggc ttacaatttg
     6721 ctgcaaactc tcaatggcag cctgacggcc aatttcaggt ttattaccag ctccacggcc
     6781 ctcggtaagg gtagcgccaa gctgaacctt aaactctacc gggctattga ctaatgcctg
     6841 ggcatcggtg ttacaaagaa caaagtcaac gtcctttatc cccatgcgat gcatgtagtt
     6901 tacggcattt cctccacctc caccaactcc aattaccttt ataattgatt ggtttataat
     6961 tggcaggtca aaatttatta tatcgtcacc cataacattc atttttaagt ttcacacaaa
     7021 caccattttt aatagaaggc acaagacaat agcaggttag tttatatcat cctcggcaaa
     7081 gagtttggag agcacatcgg ttattttcat tcccagagta cgtttattga tttgctttga
     7141 ctcctttgcc tgaacctcgg caaattcttc ggttttattt tcttcgattt tggtttccgt
     7201 ggttgtacag ggtgaggctt cgcctgcgtc gatatactcc agccccatta aacccagacc
     7261 aataacggta gagtattgtg ggtagttata attttcaagc gattcggagc ataacatctc
     7321 agaggggtaa ccaatacgaa catcgtagcc ccaattgaat tttataagcg aaacaatatg
     7381 ctttagcagc gcgcctccac cggttattac tacaccggct gtaagcttat tggcaaagcc
     7441 cgacatctga atttcgtaca tggcatattc aagtatctcc tccatgcgag cctgaataat
     7501 tcctgcaagg cttcgcatcg atatttcctt gggttccctt ccgcctattc ccggaacggt
     7561 gataattttg ttatcggggg ccatgctcat cagtgcatat ccgtgatgga gctttagcat
     7621 ctcggcgtgc ttttgaagta tctgacaacc ctccttaata tcctgcgtaa taattgaacc
     7681 gccaatggga ataactgccg tatggcgtac aatgccgttg tagtaaacgg ccaaatcggt
     7741 ggtgccacct cctatgtcga ttagggctac gcctgcttcg cgctcctcgt cggtgagcac
     7801 agccttggat gaggctaagg gctcaagtat aagttccgat acctttaccc ctgcacgatc
     7861 cacacacttt tcaatgtgct ttgccgatga ggttttacca attactatgt ggaagttggc
     7921 gccaagcctt cggcctagca tgcctatggg tttatcatcc tctatcttta cattatcgat
     7981 tgagaaatcc agcggcaata catgaagaat gctatcgcct gtatcaacag ggatattata
     8041 catatcctca ataagacgct tgatgtggct ctttttgatc tcagtttctt cggaacccag
     8101 cgtgatgtag ccatagtttt taatgctgta gatgtgttgc cctgctattc caacaaaggc
     8161 cgatttgatt ttaacgccag cctgcgattc ggcctcgcga acggtttttt caacggcctt
     8221 tactgtttcc tcaatgtttt gaatcattcc cttttttata ccagtggaat cggtggagct
     8281 aaagcccaca atctgcaagc gtccgtttgt ggtgcgtttc cctattaagg taactacctt
     8341 ggttgtacca aggtcaattg cagctacgta agtgttgttg tcctgtgcca tagttgttag
     8401 tattttgtgc aaactatttg atttttaaat tttaggttaa ttacggtgta tttgttccag
     8461 ccttcggctg gcaacgcttt ctcgtaaaag agtttaagtt tcttgagctt aacctcgtag
     8521 ttatccaaat tgcccagtac aatgatatgg ggcccaacca agggaataag ctcaatgtta
     8581 ttcctgttcc taacgtaaac ctgttctatt tgagcattcc agaacttgtc gttgtttaca
     8641 tacattgcaa actcgtaaag cttgtgtata aagttttttg gaccttcctt tgagctatcg
     8701 gtaaatgctg taacatcaag gttgggcaca accttaaagg gctccacgat attaccgttt
     8761 gccaccagcg tgttagcagc aaaccccgat gagtagggta gtattttacc agaatcgtcg
     8821 atatagtagg tttgcccttt agcattgaaa actcttaaaa cgggttggcg ttgagtaatt
     8881 tcaactatta cttcgccttt agcggtttta aatgcttgaa catcgcgcac agcagccatt
     8941 tggtaaatat cctgttcaat tctattggta tttatgctgt taaggctctg tcctataggt
     9001 gagttttttg attttacaat tgttctgtat acatccttag ccctcacaaa gttaagtttt
     9061 gcgctatcga gaactcttac ctcaagtttt gtgcagcgtt tccctttata cactttattg
     9121 gcataggaaa gcccataaat aacccaggcc agaacaaaaa gccaagccaa aactttaagc
     9181 cctatgcgca acttcctatt catttatagc tctattctcc tttgcttcaa gtatgttaac
     9241 tatctcgttt atcattctgt cgatatcgcc tgcaccaatg gtgaggagaa tatccacatt
     9301 attgcatttt aaccagctaa ttaattcggc ctttggaaca acttgcgcat tactgttact
     9361 catcagcttc attatggttt ccgatgtaac cccttcaatt ggaagttccc ttgccggata
     9421 tatttcggtg ataattggtt tatcaagctt gtcgagtgcc gcagcaaact ccgctgcaaa
     9481 gtcgcgggtg cggctgtaga ggtgaggttg aaatatacca gtgatttttc ggttagggaa
     9541 aacctcacga accgatctta ccaacgcttc aatttcgctg ggatggtgtg cataatcgtc
     9601 aatgtagatg atttttgttc ccctatacct tacatcaaag cggcgttgaa cacccctaaa
     9661 cgactctaag ccgtgagcta acttttcagt cggaacgcca agggttaacg ctgctgccga
     9721 tgcagcaatt gcattctcca ggttgtaatg gcctggtact cctagtgtaa gattgtttat
     9781 tgaaccctgg ggagttacaa gggtaaacct gtaaagtcca tcgtggatgc taatgtttgt
     9841 tgggtagtaa tggcagtcgg gcgtaaatcc gtaagtaatc acttcagttt ccttgcccac
     9901 aataggtttg aatgcaatat tttgctttac aaccaaatac ccgccgggtt ttatttgcga
     9961 cacaaattcc tgaaaagcat taagtactgc atcgtaagtg ccgtaaatgt caaggtggtc
    10021 agcatcaacc gaggtaacaa tggcaagatg tgggtgaagt gtaaggaagc tacggtcgta
    10081 ctcatcggcc tctaccacaa gtagctgctt gccttggtcg gcaagtatca ggttgctgga
    10141 gtagttgcgc gatatgcctc ccaaaaaggc atcgcaaccc attgatgtag ccaaaaggtg
    10201 ggccaacagg gttgatgtag tagttttacc gtgagtgccc gcaacggcaa gggttttata
    10261 gtttgaactt agtaatccaa gaacctgtgc gcgtttcatc agtacaaacc tattgctgcg
    10321 gaaatattca agctcgctgt ggtttgcagg aatagctggt gtatagataa caagagtttc
    10381 ttccctgttc aggaatgctc ttggaatagc cccaacgcta tcggtgtaat ggatatttgc
    10441 ccctgcagct tcaaggtttt gggtaatatc ggacgaaacc ctgtcgtacc ctgcaacagg
    10501 aaccccgtag tgcaagtagt aacgggcaat ggcactcatt ccaatccctc caatcccaag
    10561 taggtaaacc ctttttactg cattcagctc catcggttca attttttgtt tttttaatac
    10621 ctgccagttt taaaacttca agggctatag ttatagccga gtttcttagt gccaaacggg
    10681 caatgttttc cgataatttt ttttgttctg cagggttgtc aagcagttca aaggctgctt
    10741 taaccagttg ctcttttgcc tgactatcgg gtatcataat ggctgcatcg cgcgaaacca
    10801 gtgcctgggc gttacgggtt tggtggtctt cggcaacatt gggcgatggt ataagtatgc
    10861 aaggtttcgc aaccaggcag agttcggaaa ttgttccagc acctgctcga gaaataatgg
    10921 tgttagctga aacataggcc aagtccatgc gggcaatgaa atcgtacagc ctaatatttt
    10981 caaccggatt gctgtcaagt gcctctttta ctgtttggta gtaaagtttc ccggtttgcc
    11041 atatcaccac aatatcgtgt cgggctttta ttaggtggat cgaagccatc aggctttggt
    11101 taatagttcg ggctccaagg cttccgccta aaacgagtaa aacctttttg tcggctccaa
    11161 tgccaaaatg ttcaagtccc tgattgcgtg taacggaaag gttttcaata tcctgccgaa
    11221 caggattgcc ggttaatttt attttttgtg cagggaagta tttttccatg ccttcgtagg
    11281 ctacacagat agttttagcc tttttggcta acagcttatt ggtaactccg gcataagaat
    11341 tctgctcctg tagtagcgta gggatacctt tgcgctgtgc agcctttaca ataggtccgc
    11401 tggcatatcc tcctacacca accacaacgt gaggtttaaa atgcttaact atacggtttg
    11461 cttttactaa gctcttaatc agcttaataa tcacaatgag attttttggg gtaaggcttc
    11521 gtgagaaacc ggcaacaggc aagcctacga tgttgtatcc ggcttgaggc accttatcca
    11581 tttccattcg gccttcggct ccaacaaaca gaatctcagc atcgggtttt tgctcccgaa
    11641 tggcattggc tatggaaatg gcagggaata tatgcccgcc agtaccccca ccactaatta
    11701 ttatccttaa ccggttcttc tccatcgaat agttcttttt tatcttgact tcgactaata
    11761 cccagtataa tacccagtgc accactgcta actatcagcg atgaacctcc caaacttaca
    11821 agtggtaatg gctgacccgt aacgggaatg gctccaaccg caacggccat attaattaat
    11881 gcttgcagcg taagcattaa ggccaatcca aaggcaagga atgctggaaa tagccggttc
    11941 gattttttaa ccagcagggc tgcccggtaa agtagcataa gatagagtat tagggtaaat
    12001 acaccgccta ccacaccata ctcctcaata attattgcgt aaataaagtc ggaatagggg
    12061 tgtggcaaaa agttacgctg ggttgagcgt ccaggccctt taccaataat acccccagtt
    12121 gcaatggcta ttttcgattg ctccagctga tagttcccct ctgttttgtc gtcgctaata
    12181 aaattctcaa tgcggtttac ccaggtttga gctctgctca gggttttaaa ctgaagcgat
    12241 atggcaataa aggctcccac cagtacaata cctatcccag taattccgaa aaggtatttt
    12301 aggttaactc gccctacaaa catcattaac caacaggtta agccaagcaa agctgcggtt
    12361 gagaagtttg ccggtaaaat tagagcgcac acaaatgcta cgggtgcaat tagctggatt
    12421 aagaattgtt taaaatcatc agtgttttcc tgcatttgcg agaggtactt ggccacatac
    12481 attattaaaa tcacctttgc taggtccgat gactgaaacg aaatgcccaa tccgggtatc
    12541 tccagccaac gcgatgcagc attgaggtta cgtccaaaaa gaagtgtaat accgagcaag
    12601 ggaactgaca atgccagaag tatattggca agcctatacc aaacgctgta ctttatgaga
    12661 tgcgcagtcc acattactat gaatgcaacc agcaggaaaa caagctgttt aatcaggaaa
    12721 taggttgtgt ttccaccatg atagcggaaa gctagtgagc tcgatgagct gtagatgagc
    12781 aggagcgatg aaaggtaaag gaagataaca atcatccaaa tcaccctatc gccctttaag
    12841 tatttatcga acagtgctcc catagctcac tgggttatat taaagcgcgc gaacagccgc
    12901 tttgaacttg cgtccacgat cctcatagtt ctcaaacagg tcaaagcttg cacaggctgg
    12961 ggaaagcagt accgtgtcgc ccggaaccga tattctgtag gcatgctgca cagcctcctc
    13021 ggccgatttg gcatcaacaa ttacgggaac aacgccatcg aatgctgcgt gaagctttga
    13081 gttatccact cccaggcaga ttaaggcttt caccttctgc tttactaaat ccataagctc
    13141 ggagtaatcg ttccctttat cagtaccgcc tgcaatccaa accacggggg ttgtcatgct
    13201 ctccagagca taccatgcgg agttcacatt ggtagccttt gagtcgttaa tgtacttaac
    13261 acctcgcaca gttagtatat attccaacct atgctctact ccctgaaaat cggaaagcga
    13321 ttcacgaata acctctttac ggatatttag tacatgacca gcaataccgg cagccatgga
    13381 gttgtaaatg ttatgcttcc ctttcaggga gaggtcgttt attgacatgc tgaaatcgct
    13441 gttatcgtaa tttatgaaca tatcgaggtt ttcgagccat gcggtttggt tctcctttgt
    13501 tttctgtgag aagggcaatt gctgggcgcg tagtacaatc tttttgagtt gctccacggt
    13561 tacagcatcg tcggagcaga aaatgaagca gtcgtcttcc gagaggtttc gggtaatcct
    13621 gaactttgat tccacatagt tctgcatctt gtagtcgtac ctgtccaggt ggtcgggggt
    13681 gatgttaagc agtatggcga tatcgggttt aaagctatag attccatcga gctgaaagct
    13741 gcttagctca ataacgtagt agtcgtagct gcattcggca acctggtaag caaaactatt
    13801 gccaatatta ccagccaacc caacgtttag accagctttc tgcatcatgt ggtaaataag
    13861 cgaggtggta gtggttttgc cgttgctacc agtaatacaa acggtgtagg cgttggtgta
    13921 tcggccagca aactcaatct ccgaaattat ggggatgccc ttttccctga ttttaacaac
    13981 cattggggct ttgtcaggaa tccccgggct tttaatcact tcgtcggcat tgaggattag
    14041 ctcctcggta tgcctgccct cctcgaagcg gatatcgtac ttgttaagcc actccttgta
    14101 gcggggcttt atttccgaca tatccgacag gaacacgtcg aatccctttt tctgggctaa
    14161 aactgcggct cctgcaccgc tttctcctcc tccgagtaca actattcgct tcatattgtt
    14221 cttttttagc ggattttcaa tgtaacaact gtaataactg caagtagaat ggctacaatc
    14281 cagaaccggg ttacaatttt gggttcaggg tagccctttt tctggtagtg gtgatgcaag
    14341 ggcgacatca ggaatatcct tcgaccctca ccatacttac gtttggtgta cttaaagtag
    14401 ctaacctgca taaccactga gaggttttcc accaggaata ttccgcaaag gattggtatg
    14461 agcagttcct tacggataag tatggcaaaa acggcaataa tacctccaat ggtaaggcta
    14521 ccggtgtcgc ccatgaatac ctgtgcaggg tagctgttgt accaaaggaa tcccaccagc
    14581 gcgccaatga atgcgctcat aaacacaact aactcgcccg aatagggaat gtacattatg
    14641 ttcaggtatt cagcataaat cacgttaccc gataggtaag cgaacacgcc cagcgtggtg
    14701 gtgattaccg ccgaaacccc ggttgccaat ccgtccagcc catcggttag gtttgcgccg
    14761 tttgatacgg ctgtgattat aagaattaca accagaataa agataatcca ggttacatac
    14821 tcgtaacctt cgcctttaaa cgggttgagt aggttgtaat caaactcgtt gttttttaca
    14881 aaggggatgg tggtttgcgt tgtcttttta gcctttgaaa cggttacggg ttgattgtcg
    14941 ttgtactccg atgttaccac agtttcaatc tgaacatctt ttttaaccct ttcaactttt
    15001 tccctaacta caatttcggg gctaagccac atcaccaagc caacaaataa cccaagcgat
    15061 acctgtccga tgattttggt acgacccgaa aggccctcct tgtttttcag gaaaaccttg
    15121 atgtagtcgt cggcaaagcc cactgctccc aaccatacgg ttgtaaccag cattacaatg
    15181 atgtagatgt ttaggatatc ggcaaagagc agtaccggca aaagcattga aagtaggatg
    15241 atgatacctc ccatggttgg ggtacccttt ttctgcattt ggccctcaag gccaaggttt
    15301 cggatttcct caccaatttg cttacgttgc aggtaaagga taatgcgctt accaattagc
    15361 aaggcaaagg ccatcgatac aatgaatgcc attgcggacc ggaacgagat gtaccggaat
    15421 aatcctgaac cgggaaaatc ccagaatttg cttatgtatt gagccagata gtatatcata
    15481 cctgcaacga tttaaaaatt tcagttagca cttctttatc gtcaaagtga tgttttacac
    15541 ctttgatttc ctggtagttt tcatgaccct tacctgcaac caggatgata tcgcctttaa
    15601 gggcaagcat acaggctgtt cttatggctt cacgcctatc cacaatggtt atcacctttt
    15661 ttgcctgcga ggcatcaacg cctattctca tatcgttaat gatttcttcg ggttcctcga
    15721 accggggatt atcggaggtg aggattacaa tatcgcttag ctccgtggcc accttggcca
    15781 taacggggcg cttggttttg tcgcgattgc cacccgcacc caccaccgta atcaggcgtt
    15841 ggttctcggt tttaatctcg tttatggttt taatcacatt ctcaagagca tcgggtgtat
    15901 gggcatagtc aactaccgca gttacgccgt gcgaggagcg aacatactca aacctccccg
    15961 aaaccggggt tacatcgctt atgccggtaa ggatatcgtt tttatcaaaa cccaaaagca
    16021 tcagggttgc ataaattgct aacaggttgt atgcgttaaa ccgacccaat agccttgtcc
    16081 aaacctcaac cccgtccatg ttaagcatca tcccatcaaa atggctttca ataaccttgc
    16141 agcggaaatc ggccatggag cgtagcgagt aggtataaac ctttgccttg gtgttttgta
    16201 ccattaccat accgttccta tcatcggtat tggttagggc aaaggcatcg gagggcaact
    16261 cgtcaaagaa gcgtttctta gccttaaggt actctgcaaa ggttttgtgg tagtcgaggt
    16321 ggtcgtgggt aatattgctg aaaatgccac cagcaaatgt caatccagct atacggttct
    16381 gaaccaccga gtgggaacta acctccatga aacagtactc gcatcctgca tcaaccatct
    16441 gccttagcag agcgttaagc tgtatagggt cgggggtagt atgggtcgat ggaacttcta
    16501 cgttatcaac atagtttacc actgttgata gtaagccagc cttatgcccc atacgcctgg
    16561 caaggcggta gagtagggta acagtggtgg ttttaccgtt ggtacccgtt ataccaacca
    16621 actttagctt ttgcgatggg tttccattaa aggccgatgc catgtaaccc aaggctaccg
    16681 atgagttatc aaccaggatg aaggtaacat ttgctggaca ttcggcaggt agttcctcac
    16741 atacaatagc cgttgctccc gatgttatgg cctgtggaat gtagctatgg ccatcgcttt
    16801 gggtgccacg tatggcaaag aaacagcaac caaccttaac cttgcgggaa tcgaatgtta
    16861 gctcatcaat atcaacattg atattaccaa ccgttttggt aatgagcttt acgggtatta
    16921 tgttttgaag ttttgccatg ttaactttct cctctgctca tttctaagta aactgttgaa
    16981 cctttaataa caggttcgcc ggggtttacc gactgtgatc gtacggtgcc tcgtccctta
    17041 gccacaaccc ttaaccctaa cctttccagc tggtatatgg catcgcgtaa tcccattcca
    17101 accacattgg gtactctgct ttttgctaaa cgaattggag ttgtagctat tttagtgcta
    17161 tcgcgctgaa cggttaccca atcggtcttt ttcccattgc tttcaaaggg tatgtcgagc
    17221 tcattcatta tatactcaag ctttgacaat tgccccgttt tggtttgagg taaatccttt
    17281 agttcatcgt ccttaatatc gctaaaccac tcaggactag tagcaaaaac cttatcggaa
    17341 atctccttga aaatgggtcc agccaccaca ttaccatagt aaacactatt tgaaggagag
    17401 ttcacaacaa caatacatga gtattttggg tcctcggctg ggaagtagcc tacaaacgaa
    17461 gcctggtagc taactcgccc tcccgatttg taacccttac gtcccttggc tatttgggca
    17521 gtacccgttt tacctgctat tttgtacctt ggatttttaa ggttggttgc tgtaccactt
    17581 tcaacaacac cctccagcac ctcatgtacc tttttaagag tggaacgcga gcaaatggac
    17641 gattggatta cctgtggcgg aaaactctca accacctgtc cgtgctttag gtatgctttt
    17701 accagtcggg gtttaaccat gcggccatta ttggcaatgg cgttgtagaa ggtaaggatt
    17761 tgcagggggg taagacgaat ctcatacccg atgctcatca tgggcatcga taggccagac
    17821 caacccttat cgcccgggaa ccggatttgg ggctttacct cgcccggtat gataataccg
    17881 gttggctcgt ttaactttaa ctcatagagt ttttttacaa aatctttctc cttgcccttg
    17941 aacacctttt gaaccagctt tattacaccc acattcgacg aaacctcaaa aacctcctta
    18001 acggtaatct tcccatggcc gtcctccttt gagtcggaaa tcacatggtc gtatagctgt
    18061 agttttccct tgccggtatt taccgtatcg ttgaggttaa tatcgccctc ctcgagcagg
    18121 gcaataaggg tggcagtttt gatggttgat ccaggctcgg tgctttcgcc cacggcgtag
    18181 ttgtaaacct cctcgtaggt tccatcctca ttacgccgga ggttagcaat ggctttaata
    18241 tcgccggtgg ccacctccat cactatggcg catccatgac ctgcaccatg cccttcaagg
    18301 tgtttgcgca atgccgcctc agccacatcc tgtagctgca catcaagggt ggtaagaata
    18361 tcaacgccat cttcgggttc aaccttgttc atgctattta cctcaaccca tgtattgccg
    18421 ggtatccggg catgaacacg caatccattt ctgcctctta gcactggatc aagcgctttt
    18481 tcaatcccaa caacccctcc atcgaggttt accataccaa gggtgcgggc tgccatggag
    18541 ttgtgcggaa gcattcgctt gtttacctgt tcggcaatga acccaccctt gttctccccc
    18601 aagcgcagca acgggaattt cgatatttcc tgtagctcaa ggtaatttac cttacgaggt
    18661 gctatggggt agtagcggcg attcttagca tacttgcggg cattggtaag ctcagagcgg
    18721 tacgagtacc acgaccggtc gccaaagaat tgcgataggc atattgccag tgagtcgata
    18781 ttagctttga acactgagtc ggttaggccg gctgccagta aatccatgcg gaggtcgaag
    18841 taaggcaccg atgtggccaa tactctgcca tcggcagcaa ggatatcgcc tctgttggcc
    18901 tcaacaagta tatcgcggta ggttatcttt tttgctttct ccctgagttc gtgcccatca
    18961 actacctgta tgaaaacaat tttgaaaacg atacctatac ccactagtag cagtaggatg
    19021 taaattcctg caatccgaat tagtatgttg ttctttactg tcataggact gctttactaa
    19081 tggttacttc agctttttgg gcggaaccct cgattctatt aggccaagcc ctttctcctc
    19141 aaccagctta accacttccg actgtcggct tatcgacatg tactgggcag cggtagttat
    19201 tgattcggca cggaggtttt
//
LOCUS       DAOOWJ010000047        18508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3017_length_18508_cov_7.592695, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000047 DAOOWJ010000000
VERSION     DAOOWJ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 18508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18508
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3017_length_18508_cov_7.592695"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PLW22_06245"
     CDS             complement(<1..969)
                     /locus_tag="PLW22_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOU74340.1"
                     /translation="MNRKLALLSIFGLLLLLACKEKIESGVSQQLANQRKQTLSNIEY
                     NIYIDIPESENAPVPGRVELMFELNERYNLPIDFRNADSCLLNVTVNYRQISANVNNG
                     HVIIPKSYLYQGQNKIAIDFLAGKTSLNRKGDYLFSLLVPDRASTVFPCFDQPNLKAS
                     YNLTLQTPKDWVAVSNSPVINKMETDTSNLWIFDKTYPISTYLFAFAAGKFKAIDFVA
                     DSFKMTIYHRETDTQKVARNIKKVFELHQSAIKWLENYTRINYPFQKLDVVLIPDFPY
                     SGMEHQGAIFYRDSRILLDENSSVTQQLNQANLIAHEVSHQWFGNLV"
     gene            1074..1520
                     /locus_tag="PLW22_06250"
     CDS             1074..1520
                     /locus_tag="PLW22_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU74341.1"
                     /translation="MNEFNVEILNLPENLYRAAIAIRGEITRRLTEAFGEEFTADYWF
                     IMNHLFKENPLTQGRLAELTNRDRASLSRTIACMERIDLVTKMAEPFDKRKELISPTK
                     TAINFKPEGERIVLQAVGMVMHNLKPIEVIELNRMLGQLFENITKG"
     gene            1626..3698
                     /locus_tag="PLW22_06255"
     CDS             1626..3698
                     /locus_tag="PLW22_06255"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOU74342.1"
                     /translation="MLVLLAVSCQEKADKNPLLSKFDTPYQTPPFDKIKHEHYMPALD
                     SAISVARHEIDAIVNNTEEPTFENTIEALDHSGKLLADVSNILFNLNEAETDSVLQQI
                     VIEASPKLTDFSNDINLNPELFKRVKAVWEKRDSLKLTPEQMMLVDKTYKGFVRNGAN
                     LSEADKEKYRAISRELSELTVKFNQNVLAETNSYKLHITNEADLAGLPQSLIDAAAYT
                     AKQKGLEGWVITLDYPMYGPFMKYADNRELRKQLYMAYGNRCFKGNEYDNQKIAQRIA
                     NLRLELANLLGYPNFATFVLENRMAETPERVNQFLDQLVAASLPAAREEVKEVEEFAR
                     QQGFKGKLERWDWSYYSEKLKNAKYSYNEEELKPYFQLEKVIDGVFLLANKLYGLTFL
                     PNGKIPVYHPDVKAYEVYDGSGRFMSVLYLDFFPREGKSGGAWMTSFRSQYRENGKDI
                     RPIVSIVTNFTKPTDKQPSLLTFYEFSTFLHEFGHALHGMLTDCNYSSLSGTSVYRDF
                     VELPSQIMENFAVEKEYLDLFAVHYQTGEKIPQELVQKIIDSRNFQAGYFSLRQLGFG
                     ILDMAWHTITKPVTEDVDSFEKKILNPLDVLPPVKGTNMSVTFGHIFEGGYAAGYYGY
                     KWAEVLDADAFELFKEKGIFNREVAQSFRENILSRGGSEHPMTLYKRFRGQEPTIDAL
                     LKRSGLKK"
     gene            complement(3800..4417)
                     /locus_tag="PLW22_06260"
     CDS             complement(3800..4417)
                     /locus_tag="PLW22_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74343.1"
                     /translation="MRKIFLTAAIALYAINSLQAQLVQDLYRIYEPIYRSSETDSTAV
                     FTPFGIVKPGTNYGLSMGTGYSFLGNGIGMSGSYIAPSVTYSNNRLQVIAGVTLSHTN
                     MHGLRPDGANFAQPYSGANPYQAWAYTQYQFSNRFSVYAMGAVSQNQTLFSPFNSYMG
                     TFNSQQFGVGFNYRLGSKTTIGASFNFVNRNPNNLFYNPNSPFGW"
     gene            complement(4674..6851)
                     /locus_tag="PLW22_06265"
     CDS             complement(4674..6851)
                     /locus_tag="PLW22_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOU74344.1"
                     /translation="MKNNDLNFKAFELSKSSLMKSIQELQPDSKANQVVEALDMAKSI
                     YEKHGEWTDSIATHLVNVAQICLNEIGLGTTSSIAALLHETIERKWLTPVEIEKKFGK
                     AVASIVLNFTQISGLDPKDPKTQAELFRQMLISLSSDARVILIKLADRLDTMRSLSKE
                     QPDVRLKRSWETFHLYAPLAHRLGLYRLKSEMEDLAMKHIYPKEYKYIIKRLQETTAS
                     RNRFLHSFMAPIEEELQRRGFTFTIKGRTKSIYSIFRKMQNQEVDFDEVHDIFAIRII
                     LDTKPENEKSDCWQVYSIITDKYTPNTERLRDWISVPKSNGYESLHITVLGPENKWIE
                     VQIRTVRMDEIAEKGLAAHWKYKGIRQEQGIDEWVARVREILESPDTSPEDKVEQFQM
                     NLTSSDIYVFTPKGDIRKLPAKSTVLDFAFDIHTEVGSQCIGAIVNGKNTTIKQELQN
                     GDVVEILTSKKQKPKKDWLTFVITSKAKSRIKQQLRDIENSEIQEGKEILLRRLKNWK
                     IDDVDEALRKVNKHLKLKKPTDIFILIAQGKVDLTAIKTLLTAEEEKSAVKEDIPKPE
                     AEKAEVKESSDYLVIDEKLVNIEYKLAKCCNPIFGDDIFGFVTVNEGIKIHRTSCPNA
                     GQLNQRWPYRVVKARWKGSSKAGAFQTTIKVTGTDEIGILNRISEVISKDLRVNMRNL
                     NVSSRDGLFEAQIQLYVEDTKHLEMLLYRLGRVQGVHKAIRLK"
     gene            7074..9083
                     /gene="ligA"
                     /locus_tag="PLW22_06270"
     CDS             7074..9083
                     /gene="ligA"
                     /locus_tag="PLW22_06270"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOU74345.1"
                     /translation="MDSQKALERIGFLRNELNRHNYLYYVKAQPEISDYDYDQLLREL
                     IDLEKQFPEFDDPNSPSRRVGSDISQEFVQVRHKYPMLSLANAYSYSELTDFDNRIRK
                     ALPEPFQYVCELKFDGVAIGLTYRNGRLVQAVTRGDGTMGDDVTVNVRTIRTIPLELH
                     GADFPEEFEIRGEIFMPRNVFDEINSEREDIGETPFANPRNAAAGTLKLLNSAEVARR
                     KLDCTLYYLLGEKLPADNHYENMLKAKEWGFKVSEHMTLCNNLTEVQHFIEHWDKARK
                     KLPFDTDGVVIKINSYRQQNILGLTAKTPRWAVAFKYKPERVSTELLSVDFQVGRTGA
                     VTPVANLKPVKLAGTTVKRASLHNADQIQLLDIRVGDWVFVEKGGEIIPKIVGVDQSK
                     RSLFSKPIDFPGACPECGTTLVRPEGEARHFCPNQYGCPPQIKGRIEHFISRKALNID
                     GLGEETVALLFDNNLIRDAADLYSLTREQLLALDRFADKSADNAIMSIEKSKSVPFPR
                     VLFGIGIRYVGETTAKKLAQHFGSIDAIATATVEQLVEVEDVGDRIALSIIEFFSDER
                     NRMLIDKLKAAGVQMAVSSADTSLLSDRLKGMSIVISGTFSRVSRDELKELIARHGGK
                     NVSSISASTSLLIAGENMGPAKLEKATKLGIKIIDEEEFFRIIEG"
     gene            9151..9708
                     /locus_tag="PLW22_06275"
     CDS             9151..9708
                     /locus_tag="PLW22_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74346.1"
                     /translation="MFDNLKYNKSLKELGKLTKFLKRQRQMYNLTTARHVGIVFNLTD
                     SKVFDAVVGFKSMLERIPITVEAVGYFNDKEVPQLYTMEKGIKVFSRGELNWYKKPEA
                     TVVSEFVAKDFDILIDLTQDEVLPLRWVSSLSKAKFKVGALNYFNNPFDLIVTVDKSL
                     GLEYLTQQIYNTLEVLNNRFAQQTV"
     gene            9719..10603
                     /gene="dapA"
                     /locus_tag="PLW22_06280"
     CDS             9719..10603
                     /gene="dapA"
                     /locus_tag="PLW22_06280"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOU74347.1"
                     /translation="MDTAKLRGLGVAMVTPFSYNYQIDYPALDKLIEFLIGNGVNYLV
                     VQGTTAETATLSDSEKRQLADYVVKKVNGRVPIVLGIGGNDTHKVIESYTKFDLTGID
                     AILSVTPYYNKPTQKGLYLHFKTIAQNTHLPIIVYNVPGRTGVNMTAETTLKLAHEFQ
                     GKIVAVKEASGNLTQMGYILRDRPSNFLVLSGDDGLTLPQISMGADGVISVLGNCAPA
                     KFSRMVNLALQGNFTDAAELHLGLIELIDLLFVEGNPGGVKAALNALGLIENVLRLPL
                     APVSDSTYDKIKKEIEKL"
     gene            10734..11720
                     /locus_tag="PLW22_06285"
     CDS             10734..11720
                     /locus_tag="PLW22_06285"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOU74348.1"
                     /translation="MANLSTSYMGLNLRNPLIVASSGLTENIDDIVEFEARGAGAVVI
                     KSIFEEEIIAKAHDNLNKMQASGFIYPETMEYFDYDSMEDPISNYLDFLRKVKSETSI
                     PIFASINCVTSEGWIDFAKRIEDTGVDGLELNVFSLPSDPDRSAEENERIYFEIADRV
                     TSVVKLPVAMKVGYFNGALANFLVRLSSTPIKALVLFNRFYNPDFDVNTLEFTPASVF
                     STPSEISTSLRWVAIMSGRVSCDIAASTGVHDGNAMVKQILAGANAVQACSTFYRNGK
                     EQASFIIRQLEEWMNSNGYKTIDEFRGKMSQKKTYNPAAFERVQFMKYFHDL"
     gene            11922..13322
                     /locus_tag="PLW22_06290"
     CDS             11922..13322
                     /locus_tag="PLW22_06290"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74349.1"
                     /translation="MRDESSDYLSFEELFNLVERFDESIQSGRAQFFDVHEFVELIDF
                     YLDHEDDEKALVALKHAENIYPGSIEIKYKWAEYYHFTGDNEKALELIHEVEKIEKFN
                     ADLLYVKGEVLFELGRLNAAVECFDKAVSADTDDNIELLHRISTYFLENEEINLALKY
                     LLISYNKEKNNLAVLFDMGYCFERLNELDKSVKYYNEYLDINPFSAPAWYNLGIVHTK
                     LGEFDKAIECYDFCIAVDPQYSSAFHNKGNTLASLEKYTDALKVFNELAELESENPRV
                     FALIGECYEKLEQFDKALEAYNKSIVIDPDFAEGYYGIGVVLAARKKYDLSLNFIRRA
                     ITLNPEEYDFWLGLGRVYFETNDLENSLKAYREATSLNPDLPDAYLSLAEVLLYEERF
                     TEVEQLIDGLGNKFDSNVSIKIINAAALYLSHRPKEALDILKDAKSIDPSSIDDFINL
                     VNPDADKEFIENINKL"
     gene            13446..14216
                     /locus_tag="PLW22_06295"
     CDS             13446..14216
                     /locus_tag="PLW22_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HOU74350.1"
                     /translation="MNYNLSFIPARTQKPREYGLTMVMDKGLSMSEAQSLIESSGQYV
                     DFVKLGFGTSLVTNNLKEKVKLYKNAGLRPYLGGTLFEIFIIRGMFDDFVKFVDELEL
                     DLVEVSDGSMELDNHVKCDYISKLAKRYTVISEVGSKRADVHIPDEEWINMMKHELSS
                     GSWKVIAEARESGNIGIYNQDHSANTKLIDDIVRHVKVENVIWEAPLKSQQAWFIKML
                     GANVNLGNIAPNEVISLETLRLGLRGDTFFQFLPDELK"
     gene            14227..15201
                     /locus_tag="PLW22_06300"
     CDS             14227..15201
                     /locus_tag="PLW22_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOU74351.1"
                     /translation="MIKRGIKDYILLVLKGIGMGAADVIPGVSGGTIAFITGIYEELI
                     DSIKSVVAPESLKYLKSFSLLSYLKAINADFLLAVIGGIAISFLSLAKLMQYLLTNHP
                     IFIWSFFFGLILASVWFVAKIIEKWNVSTYLFFITGIILGFAITTITPTETPNDLWFI
                     FICGVVAICAMILPGISGSFILLLMGKYIYMMSALSSFNFPIILVFLLGAAVGILAFS
                     NFLSWLLHHFHAATIAVLAGFMLGSLNKVWPWKVATQTFIDAQGIIRPLIEKNVLPST
                     YLIENKAEPYLLGAIGFAILGAIIIILFERFFSKPVTKEVDLREKLGL"
     gene            15191..16015
                     /locus_tag="PLW22_06305"
     CDS             15191..16015
                     /locus_tag="PLW22_06305"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOU74352.1"
                     /translation="MVCRAKIVGYKGLLLPNKVKAILFIFLVSWGVNAQEKPLVYPFR
                     VGEVVKYNAYYNWQFVWLNAGTAEFYINDTLVNGVPAYHFKSLGASLNSYDWFFKVRE
                     RFESIAKKENLAPLWFIRESYEGGYKVFNRYSFSYSNSKLVIDSYTSNRPYKQNTFTI
                     DKPIFDVLTAIYYCRTIDFKRLNKGDLVPLTMAVDDGVYNLYIRYLGPENIKHRNGNS
                     YDTYKFSVKLVEGTIFKGGEDMIVWVSRDEKKIPIVVEAKILIGSVMAYLLEYKVN"
     gene            complement(16039..17031)
                     /locus_tag="PLW22_06310"
     CDS             complement(16039..17031)
                     /locus_tag="PLW22_06310"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOU74353.1"
                     /translation="MGKVLAHSDTLKPLTDGTVKGFRFSVSQKTNGRKAWHNRHGMPY
                     VGIALTGIDLGNKRVLGNAWGIQPFISYQLLQFKKFSVRTDFGLGLAYITRKYSPSHN
                     PTNVAISTSINYWATANLTSSFKFSNQFNLTAGIETNHFSNGAIKKPNYGLNIVGLSF
                     GFTYNPERDKLDNCKKEVYLENSDSKSQLGIVILAGIKETGPAGGEKYYPLSISMEYL
                     MPLAQSYPLMAGLDIMFDKSTRFHIELSGKHYSPVNDDFQVGIKTGLQIPFNRLSLHG
                     HLGWYLYNKNPRLPLYYQKVGIRYRLSRTSQVQLELKTHLNIADHVGVGYAISI"
     gene            complement(17226..>18508)
                     /locus_tag="PLW22_06315"
     CDS             complement(17226..>18508)
                     /locus_tag="PLW22_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU74354.1"
                     /translation="WGYDFNHESAETQKFVDSFLTYWLTEYKVDGFRFDFTKGFTNTP
                     NAGSAYDATRIAILKRIADKIWSVNPNAYIILEHFCDNSEEMALAEYRSSEGKGMLIW
                     GNMNYAYGEATMGWVSTSDISWVAAKQRGWSVPHVIGYMESHDEERLMYKNLQYGNAS
                     NSDHNVKTLPIALMRMQTAANLFIPFPGPKMIWQFGELGYDVSIDYNGRLGRKPLHWE
                     YYQDDNRREIFNTFAHLNRLKQQYEVFKTDNYDYNLSGAIKWLKLNSSAMDVVIAGNF
                     DVNQKSTTITFNNTGWWYEYYSGDSVQLSTTSYQLTLNPAEYRMFTSERIKRNDIYVG
                     INQPKSSKAEIMVWPNPASGNIFIKYFQPSNSHVKVDMYNLTGQLVYSETRHNFSGEN
                     TIGINIPNELPRGLYILRISSNGFVNSSKVAIEH"
BASE COUNT         5238 a         3587 c         3965 g         5718 t
ORIGIN      
        1 aaccaggttc ccaaaccact ggtgcgaaac ctcgtgagca attaggtttg cctggtttag
       61 ttgttgggta accgaggaat tttcgtcgag caaaatacgg ctatccctgt aaaatattgc
      121 tccctgatgc tccatgccac tgtatggaaa atcgggaatc agaaccacat cgagtttctg
      181 gaaggggtaa ttgattctgg tatagttttc gagccatttg atagcgcttt ggtgcaactc
      241 aaacactttt ttgatatttc tggctacctt ttgggtatcg gtctcgcgat ggtaaatggt
      301 cattttaaaa ctatccgcta caaagtcaat agctttaaac ttgccggctg caaaggcaaa
      361 aaggtaagtg ctgatagggt aggtcttgtc aaatatccag aggtttgagg tgtcagtttc
      421 cattttgttg attacagggg aattgctcac ggctacccaa tccttagggg tttggagggt
      481 taggttgtaa cttgctttca ggttgggttg gtcaaagcaa gggaaaacgg ttgatgccct
      541 gtcgggcacc agtaagctga aaaggtaatc gcctttgcgg ttgagtgagg tttttccagc
      601 caaaaagtcg attgcaattt tattttgccc ttggtaaagg taacttttag gaattataac
      661 atgaccatta tttacattag cactaatctg gcgatagtta acggttacat ttaataggca
      721 gctatcggca ttcctgaaat cgattggtag gttgtagcgc tcatttagtt caaacatcaa
      781 ctcaacacgt ccgggaactg gagcattttc gctttcaggg atatcaatat aaatgttata
      841 ctcaatattt gaaagggttt gtttacgttg gttggcaagc tgttggctta caccagattc
      901 aatcttttcc ttacaagcca aaagaagtaa taatccaaat atagatagta aggctaattt
      961 gcggttcatt tatttacaat tactaaaatg caaaactttt gcgaaaatac ttaatttgtg
     1021 ttaacggaaa agcttaaggt ttatttttgc actacctaaa aaattattct gtgatgaatg
     1081 aatttaatgt tgagatacta aatctgcccg aaaacttata ccgggctgca atagccataa
     1141 ggggggaaat tacaagaagg ttaaccgagg cgtttggcga ggagtttact gccgattact
     1201 ggttcattat gaatcatctt tttaaggaga atcctttaac acaaggtcgt ttagctgagc
     1261 taaccaatcg cgatagggct tcactttccc gaacaatagc ctgcatggag cgcattgact
     1321 tagtaactaa aatggcagag ccgttcgata aacgaaagga gttaatttca cctacaaaaa
     1381 ctgcaatcaa ctttaaacct gagggggaga ggatagtctt acaagcagta gggatggtca
     1441 tgcataacct taagccaatt gaggttattg agttaaaccg aatgcttggt caactgttcg
     1501 aaaacatcac caagggttaa attctcgaaa tcaagatcta tctgtacaaa aagaattact
     1561 tttgacacca aattttaaaa gaataattta ttatgctaaa acaatcaaaa tcaattctgg
     1621 gcattatgtt agtattactt gcagtatcgt gtcaggaaaa agccgataag aacccattac
     1681 taagtaagtt tgatacaccg tatcagacac caccgttcga taagataaag catgagcact
     1741 acatgccggc gcttgattcg gctatatcgg tggctcgtca tgagattgat gcaatagtta
     1801 ataataccga agaaccaacc tttgagaata ccattgaggc ccttgaccat agtggtaagc
     1861 tgcttgccga tgtttccaat attctattca atttaaatga ggctgaaacc gatagcgttc
     1921 ttcagcaaat agtgattgag gcctcaccaa agcttaccga tttctccaac gacatcaacc
     1981 ttaaccctga gctctttaaa agggttaagg cggtttggga aaagcgcgat tccctgaaac
     2041 tcactcctga acaaatgatg cttgttgata aaacctacaa gggttttgtg cgtaatgggg
     2101 ctaacctaag cgaggccgat aaggagaagt atcgtgccat tagccgcgag ctttccgagt
     2161 taacagtaaa gtttaaccaa aacgttttgg ctgaaactaa cagctataag ctacatataa
     2221 ccaatgaggc cgatttggcg ggcttacccc aaagccttat tgatgctgct gcctatacag
     2281 ctaaacagaa agggcttgag ggttgggtta ttacacttga ctaccccatg tatggcccat
     2341 ttatgaagta tgccgataac cgtgaactcc gtaaacagct ctacatggct tacggtaatc
     2401 gttgtttcaa gggcaatgag tacgataacc aaaaaattgc tcaacgtatc gcaaatctta
     2461 ggttagaatt ggcaaacctt ctaggttatc caaacttcgc cacctttgtc cttgagaacc
     2521 gcatggccga aactcctgaa cgagtaaatc aattccttga ccagctggtt gccgcatcgt
     2581 tgcctgctgc tcgcgaggag gttaaagagg ttgaggaatt tgcccgccag cagggattta
     2641 agggcaaact tgaacgttgg gattggtcgt attattccga aaaacttaag aatgcaaagt
     2701 atagctataa cgaggaggag ctaaagcctt acttccagct cgagaaggtt atcgatggtg
     2761 ttttccttct ggccaataag ctgtacggac ttaccttttt acctaatggt aaaattcctg
     2821 tttaccaccc cgatgtaaaa gcatacgagg tgtacgatgg tagcggacgt ttcatgtcag
     2881 tcctatacct cgactttttc cctcgcgagg gtaagagtgg aggtgcatgg atgacctcat
     2941 tccgttcaca gtaccgagaa aatggcaagg acattcgccc aattgtttca attgttacca
     3001 attttactaa accaaccgat aaacaaccat cgttgcttac tttctatgag ttttctacct
     3061 tccttcatga gtttggtcat gcactgcatg gtatgctaac cgattgcaac tactcatcgc
     3121 ttagcggaac ctcggtttat agggattttg tggagttgcc atcacaaatc atggaaaact
     3181 ttgcagttga aaaggaatac ctcgacttat ttgcggtaca ctaccaaacc ggcgagaaaa
     3241 tcccgcagga gcttgttcaa aagattattg atagccgtaa ttttcaggca ggctactttt
     3301 cgttacgtca gttaggtttt ggaatactcg atatggcttg gcacaccatt actaagcctg
     3361 taactgaaga tgttgacagc tttgagaaaa agatacttaa tcccctggat gttttacccc
     3421 cggtaaaagg cacaaatatg agcgtaacct ttggccatat ctttgagggt ggatatgccg
     3481 ccggatacta tggctacaag tgggccgaag tgctcgatgc cgatgctttt gaacttttta
     3541 aggaaaaggg aatatttaac cgtgaggttg ctcagagctt cagagagaat attttatcga
     3601 gaggtggcag cgaacatccg atgacccttt acaagcgttt ccgtggtcag gagccaacaa
     3661 tcgatgcgtt actgaaacgt agcggattaa agaaatagcg atattggggt taatataaaa
     3721 aggctgctca gcgagcagcc ttttttacaa tatgtttcga tgaaaaattt ttgagtattc
     3781 caaaaatttg agtttctctt taccaaccaa aaggagaatt tgggttatag aagagattgt
     3841 tcggattgcg gttaacaaag ttaaagctag ctcctattgt tgttttacta cctaaacggt
     3901 aattaaatcc tacgccaaat tgttggctat taaaagttcc catatagcta ttaaagggtg
     3961 agaataaggt ttggttttgt gataccgctc ccatggcata cacgctaaaa cggtttgaga
     4021 attggtattg agtgtatgcc caagcctgat atggatttgc accgctataa ggttgagcaa
     4081 aattggcacc atcgggtctt aatccatgca tatttgtgtg tgagagagta acgccagcaa
     4141 tcacctgtaa gcggttattt gagtaggtta cacttggggc tatatagctc ccagacatgc
     4201 caatcccgtt gcccaaaaag gaatagcctg ttcccatgct taaaccgtaa tttgttccgg
     4261 gcttaactat accaaatggg gtaaacacag cagttgagtc ggtctcagaa cttctataaa
     4321 tcggctcgta aattctgtat aaatcctgaa caagctgtgc ctgcaaagaa tttatagcat
     4381 ataatgctat tgctgctgtt aaaaaaatct ttctcataac tttaaaaatt tcctgccacc
     4441 aatataatgc aaaaaatgcg aaaaccgtaa atggggttgg ttaaaaaaag tttaggggaa
     4501 gggtgaaaaa gggataaagg ttaaaggata aaggataaag gataaaggat aaaggataaa
     4561 ggttaaagaa taggttgatg gttgatagtg aatacaaagg gtttgacgct ttgcaacgct
     4621 gttgcactca gtgtaactct gtggtacttt tttagttaat ggttacgtgc aaatcatttc
     4681 aatctaattg ctttgtgtac accttgcact ctgccaagac gatagagcag catttcaaga
     4741 tgcttggtgt cctctacgta tagttgtatt tgagcttcaa acagaccatc gcgggagcta
     4801 acattcaggt tacgcatgtt tacccgtaaa tccttactga taacttccga tatacggttc
     4861 aaaattccaa tttcatcggt tccggtaacc ttaatggttg tttgaaatgc acctgctttt
     4921 gacgagcctt tccaccttgc ttttactaca cggtatggcc aacgctggtt tagctgaccc
     4981 gcatttgggc aggacgttcg gtgaatcttt attccctcgt ttactgtcac aaagccaaaa
     5041 atatcgtcac caaatattgg gttgcagcat ttggcaagtt tatactcaat gttcaccagt
     5101 ttttcgtcaa ttacaaggta atcggaactt tcctttacct cagccttttc agcttcgggc
     5161 tttgggatat cttccttaac ggcagatttt tcctcctcgg cagttagtag ggtcttaatg
     5221 gccgttaggt caactttgcc ttgggcaatg agtataaaga tatcggtagg tttctttagc
     5281 ttaagatgtt tgtttacctt tcgtaaagcc tcatcaacat catcaatttt ccaattcttt
     5341 agcctacgta gcagtatttc tttcccttcc tgtatttcac tattctcaat atcgcggagc
     5401 tgctgcttta tcctactttt agcttttgag gtgatgacaa atgttagcca gtcctttttg
     5461 ggcttttgct tctttgaggt aaggatttca accacatcgc cgttttgaag ttcctgcttg
     5521 atagtggtat tcttaccgtt tacaatggct ccaatacact gcgagccaac ttcggtatga
     5581 atatcgaagg caaagtcaag caccgtcgat tttgcaggta gttttcgtat atcaccctta
     5641 ggtgtaaaaa catagatatc gctgctggta aggttcatct ggaattgctc aaccttatcc
     5701 tctggcgaag tatcgggcga ttccagtatt tccctaacgc gggcaaccca ttcgtcaatt
     5761 ccctgttcct gccttattcc tttatatttc cagtgtgccg caagaccttt ttcggctatt
     5821 tcgtccattc gtacagtacg gatttgaacc tcaatccact tgttttcggg tccaagcact
     5881 gtgatatgaa ggctttcgta accattactt tttgggaccg aaatccaatc gcgtaagcgt
     5941 tccgtattgg gtgtgtactt atcggtaata atggagtaaa cctgccagca atcggatttt
     6001 tcgttttcgg gtttggtatc aagtataatc ctgatggcaa aaatgtcgtg aacctcatca
     6061 aaatcaacct cctgattttg catctttctg aaaatggagt atatggattt ggttcttccc
     6121 tttatggtga atgtaaaccc cctcctctgg agttcttcct caataggagc cataaagcta
     6181 tgtaggaatc tgttccgact ggctgtggtt tcttgcaatc tttttataat gtacttgtac
     6241 tccttggggt agatgtgctt catggctaaa tcctccattt cggactttag cctgtataag
     6301 ccaaggcggt gtgcaagtgg ggcatagagg tgaaatgttt cccatgaacg ttttagcctg
     6361 acatcgggct gctccttgct aaggcttcgc atggtatcga gcctgtcagc taactttatc
     6421 agtatcaccc gggcatcgga cgataggctg attagcattt gcctgaaaag ctctgcctgt
     6481 gtttttggat cttttgggtc gagccctgaa atttgagtaa aattcaacac tattgaggca
     6541 accgcttttc caaacttttt ctcaatctca accggggtta accatttgcg ttcaatagtt
     6601 tcgtgtaaca acgcggcaat tgagctagta gtaccaagtc caatctcatt caagcagatt
     6661 tgggcaacgt taacaagatg tgtggcaatg gaatcggtcc actcaccatg tttttcataa
     6721 atcgattttg ccatatcaag agcctcaaca acctgattag ccttactgtc gggttgaagt
     6781 tcctgtatgc tcttcattaa cgaactcttg gataattcga atgccttgaa attcaaatca
     6841 ttgtttttca tttgccctag tttgcttttc cataaaaata cgatatttag ttaatctatt
     6901 gcaaggggat attgaaaggg taaagggtaa agggtaaagg ataaaggata aaggataaag
     6961 gatggctggt tctaagtttt tattgcataa atcaattttg ttaataactc agatattgtc
     7021 ctaattcatt caatcaattt tcagcctaaa tttgaattca taaactattt aaaatggata
     7081 gtcaaaaggc tttggagcga atcggatttt tgcgaaatga gttgaatagg cataattacc
     7141 tttactatgt aaaggcgcag cctgaaatta gcgattacga ttacgatcag ctcctgcgcg
     7201 aactaattga tttagaaaag caattccctg aatttgacga tcccaattct ccatcgcgga
     7261 gggtgggtag cgatatctct caggagttcg ttcaggtaag gcacaaatat ccaatgctat
     7321 cattggccaa cgcatattca tactctgaac tgactgattt cgataaccgt atccgtaagg
     7381 cattaccgga gccttttcag tacgtttgcg aactaaagtt cgatggggtt gccattggtc
     7441 ttacctatag gaatgggcga ttagttcagg ccgttactcg tggcgatgga actatgggcg
     7501 acgatgttac cgttaatgtt cgaaccatta gaaccattcc ccttgagtta catggggccg
     7561 attttcccga ggagttcgaa attaggggcg aaatatttat gccccgaaat gtttttgatg
     7621 aaattaacag tgaacgcgag gatattggtg aaacaccctt tgccaaccca cgaaatgctg
     7681 ctgccggcac cttgaagtta cttaactctg ctgaggtggc tcgccgtaag ctcgattgta
     7741 ctctttatta cctattgggc gagaaacttc ctgccgacaa ccattacgag aatatgctta
     7801 aggctaagga atggggcttt aaggtttcgg agcatatgac cctatgcaat aatttaacag
     7861 aagttcagca tttcattgag cactgggaca aggcacggaa gaaattgccc tttgataccg
     7921 atggggttgt tattaagatc aacagctatc ggcagcaaaa catactgggc cttactgcaa
     7981 aaacaccaag gtgggctgtt gctttcaagt ataagccaga aagggtttca accgaattgc
     8041 tttcggtcga ttttcaggtt ggacgaactg gtgctgtaac gcccgttgcc aacctaaagc
     8101 cggtaaagct agctggaacc accgttaagc gcgcctcgct ccataatgcc gatcaaattc
     8161 aactgcttga tattagagtt ggcgattggg tttttgttga aaagggtggt gaaataattc
     8221 caaaaattgt tggtgttgac caatcgaaaa ggtcactttt cagtaaacca atcgattttc
     8281 cgggtgcatg tcctgaatgt ggaactacat tggttaggcc tgagggtgaa gcccgccatt
     8341 tctgcccgaa ccagtatggt tgtccaccgc aaataaaggg taggattgaa cactttatca
     8401 gccgaaaagc attgaatatc gatggtttag gtgaggaaac ggttgccttg ctctttgata
     8461 acaaccttat tcgcgatgct gccgacctat actcgcttac ccgcgaacag cttttagcat
     8521 tagataggtt tgccgataaa tcggccgata atgcaattat gagcattgaa aaatcaaagt
     8581 cagttccttt tccgcgagtt ctttttggca ttggaatacg atacgttggt gaaaccaccg
     8641 caaaaaagct tgcgcaacac tttggaagta tcgatgctat tgctactgca acagttgaac
     8701 aacttgttga agttgaggat gttggcgacc gtattgcttt gagtattatc gagtttttca
     8761 gcgatgaacg caaccgaatg ctcatcgata agctaaaggc agcaggtgtt caaatggctg
     8821 ttagctctgc tgatactagc ctattgtccg ataggcttaa gggaatgtca attgtgattt
     8881 caggcacatt ttcccgagtt tcgcgcgatg agcttaagga gctaattgct aggcatggcg
     8941 gtaaaaatgt tagttccatt tccgcctcca catcgcttct tatcgcaggt gaaaatatgg
     9001 gaccggcaaa gctcgagaag gccacaaagc tgggaataaa aattattgat gaggaagaat
     9061 tttttaggat tattgagggg tagactataa gctttttttg atttacaccg ttttttttgt
     9121 aaaattgcgt ttcaacataa acctagatag atgttcgata atctcaagta taataagtcg
     9181 ttaaaggagc ttggtaagct aactaaattc ctgaaacgcc aacgacagat gtataattta
     9241 actactgccc gccatgttgg tatagttttc aaccttaccg attctaaggt ttttgatgca
     9301 gtagttggat ttaagtccat gcttgaacgc attcccatta ccgtggaagc tgttggttac
     9361 tttaacgata aggaggtacc acagctttat accatggaaa agggaataaa ggttttctca
     9421 aggggtgaac ttaattggta taagaaaccc gaagcaacgg ttgtatcgga gtttgtagca
     9481 aaagactttg atattctaat tgatttaacc caggatgagg ttttgccgct caggtgggtt
     9541 tcatcgcttt ccaaagctaa gttcaaggtt ggtgctttaa attacttcaa taaccctttt
     9601 gacctgatag taacagtcga taaatcgttg ggtttagagt acctcactca acagatttat
     9661 aacacccttg aggttctaaa caaccgtttt gcccaacaaa cagtttaaaa atatttttat
     9721 ggacaccgct aaactcagag gtcttggggt ggcaatggtt acccctttca gttacaacta
     9781 tcaaattgat taccctgcac tcgataaact tattgagttt ttaattggca atggggttaa
     9841 ctaccttgta gttcagggaa caactgccga aacggcaacc ctttccgatt ctgaaaagcg
     9901 ccagttggcc gattacgttg ttaaaaaggt aaatggccgt gtacccattg ttttaggtat
     9961 tggtggtaac gatacccata aagtaattga atcatacaca aagtttgatt taaccggaat
    10021 cgatgctatt ctttcagtaa caccatacta caataaaccc acgcagaagg gattatacct
    10081 tcactttaaa accattgctc aaaataccca cttacccatt atagtgtaca acgttcccgg
    10141 tagaactggt gtgaatatga cagccgaaac cacccttaag cttgcccatg agttccaggg
    10201 taaaattgtt gcggttaagg aagcatcggg caacctaacc caaatgggtt atattctccg
    10261 cgataggcct tccaacttcc tagtcctttc gggcgacgac ggtttaacgc taccgcaaat
    10321 atcaatggga gccgatggcg taatctcagt tttaggaaac tgcgctccag ctaagttctc
    10381 acggatggtt aaccttgccc ttcagggcaa ctttaccgat gcagctgaat tacacctggg
    10441 tttgattgag cttatcgatt tactttttgt tgagggtaac cctggtggag tgaaggctgc
    10501 tcttaatgca ctgggcctaa ttgaaaatgt tcttcgtctg ccccttgcgc ctgtaagcga
    10561 ttcaacctac gataagatta aaaaggaaat tgaaaagcta taatcaacct ttctgattga
    10621 ataaaaggga agccacatgc ttcccttttt gctttattaa ttaatttgcc ttacatttag
    10681 tgaactcttt aaaaaattta ttgttagtca attactaatt aaatctttcg gctatggcaa
    10741 atctatcaac atcctacatg ggtttaaacc tgcgcaatcc attaatagtt gcaagttcag
    10801 gtttaactga aaatatcgat gatatcgttg agtttgaagc gcgtggtgct ggagctgttg
    10861 taattaaatc aatttttgag gaggagatta ttgccaaagc ccatgataat cttaacaaga
    10921 tgcaggcatc aggtttcatt taccccgaaa ccatggagta ttttgattat gacagcatgg
    10981 aagaccccat atctaactat cttgatttcc tgagaaaggt taaaagtgaa acatctatac
    11041 ccatttttgc cagcatcaat tgtgttacat ccgaggggtg gatcgatttt gccaaacgca
    11101 ttgaggatac tggggtagac ggacttgagt tgaatgtctt ctcattacct tctgaccctg
    11161 accgttcagc cgaagagaat gagcgtattt actttgaaat tgcagatcgt gttaccagtg
    11221 tagttaagct accggtagcc atgaaagttg gttacttcaa tggggcgcta gcaaacttcc
    11281 ttgtgaggtt gagtagtacc ccaataaaag cattggttct gtttaacagg ttctataatc
    11341 ccgactttga tgtaaacacc ttggagttca ctccggcgag tgtttttagc actccttcag
    11401 agatttccac ctcgctccgt tgggtagcta ttatgtccgg tagggttagc tgcgatattg
    11461 ctgcatcaac cggtgtgcac gatggcaatg caatggtcaa gcaaattctg gctggtgcta
    11521 atgccgttca ggcatgttca accttttacc gtaacggcaa ggaacaagcc tcatttatca
    11581 ttcgccagct tgaggagtgg atgaattcta atggatataa aactattgac gaattccgtg
    11641 gcaagatgag ccagaaaaaa acatacaatc ctgctgcttt tgaaagggta caattcatga
    11701 agtattttca tgatctttag ttcccctttt aatctttgat tcaccattat ctattgcatt
    11761 ttgcatggtt taattaaccg tgttcataag atacatttga tgttaataag ttattaccct
    11821 tttttgggga ttattttagg tatttacact taatttaact aactttacta agggtgacat
    11881 tcattttatt agtatgtttg taggttaatt atttttaaag catgagggac gagagcagcg
    11941 attatctgag ttttgaggaa ctatttaatt tggttgaacg ttttgatgaa agcattcaaa
    12001 gcgggcgggc tcagtttttc gatgttcatg agtttgttga acttattgat ttttacttag
    12061 atcatgagga tgatgaaaag gcactggtcg cattaaaaca tgctgagaat atttaccctg
    12121 gttcaattga aattaaatac aagtgggcgg agtactacca ttttactgga gacaatgaaa
    12181 aagcccttga gctcattcat gaggtagaaa aaattgaaaa atttaatgca gacctgttat
    12241 acgtaaaagg cgaagtgctt tttgaactcg gaaggttaaa tgctgccgtg gagtgtttcg
    12301 ataaagctgt ttcagctgac actgacgata atattgagct actacatcgc atttcaacct
    12361 atttcctgga aaacgaggaa ataaatcttg cgctgaaata cttattgata tcatacaaca
    12421 aggagaagaa caacttagcc gttctttttg acatgggtta ctgttttgag cgattaaatg
    12481 agctcgataa aagtgtcaag tactataatg agtacctaga tattaaccca ttttcagctc
    12541 ctgcttggta caacttagga atagttcata ccaagctcgg tgaatttgat aaggccattg
    12601 agtgttacga tttttgtatt gccgttgacc cacagtactc ttcagcgttt cataataagg
    12661 gtaatacact tgcctcgctg gaaaaatata ccgatgcttt aaaggtattt aatgagttag
    12721 cagaacttga gtccgaaaac ccaagggtgt ttgcgctaat tggcgagtgt tacgagaaac
    12781 ttgagcagtt cgacaaagcc cttgaggcct acaataaatc tatagtgatt gatcctgatt
    12841 ttgctgaggg gtattatggc ataggggttg tgcttgcagc acgtaaaaag tatgacctta
    12901 gcctcaattt cattaggagg gctatcactt taaaccctga agagtacgat ttttggctgg
    12961 gattgggtag ggtttacttt gaaacaaacg atttggagaa ctccctaaag gcttaccgtg
    13021 aggctacaag cctaaacccc gatttgcccg atgcatacct atcacttgcc gaggttttac
    13081 tatacgagga acgattcacg gaagttgagc agctcattga tggcctgggc aataagtttg
    13141 actcaaatgt ctcaatcaaa ataatcaatg cggctgccct gtacctatcg catagaccca
    13201 aggaagctct tgatattctc aaggatgcaa aaagtatcga cccctcatca atcgacgact
    13261 tcataaacct ggttaatcct gacgccgata aggaattcat tgaaaatatc aataaacttt
    13321 aaatcatacc attacggaag ttaacttttc cgtagaaaaa gcattgttaa taatttgctg
    13381 catgtaaatg cagctttttt attttaaaaa tcatacattt ggctttaaac aaaaaatttt
    13441 ttgctatgaa ttataactta tcgttcattc cagctcgaac ccaaaagccc cgtgaatacg
    13501 ggttaaccat ggtaatggat aagggtttaa gtatgtccga ggcacaaagc ctgattgaaa
    13561 gtagcggtca atatgtcgat tttgtgaagt taggttttgg aacatcgctg gtaactaata
    13621 atttgaagga gaaagtaaag ctttataaga atgcaggcct tcggccttac cttggaggta
    13681 ccctattcga aatctttatc attaggggta tgttcgacga ctttgtaaag tttgtcgatg
    13741 agttagagct cgacctggtt gaggtttccg atggctcaat ggaacttgat aatcatgtta
    13801 aatgtgatta tatcagtaag ttagccaaac ggtatactgt tatttctgag gtaggatcaa
    13861 agcgtgccga tgttcacatt cctgacgagg agtggataaa catgatgaag catgagctat
    13921 cgtccggcag ctggaaggtt attgccgagg cacgcgaaag tggaaatatt ggcatttaca
    13981 accaggacca ttcagccaac actaagctaa ttgacgatat tgttaggcat gtaaaggttg
    14041 aaaatgtaat ttgggaagcc ccacttaaaa gtcaacaggc atggttcata aagatgctag
    14101 gtgctaacgt taaccttggg aatatagctc caaacgaagt tatttctcta gaaaccctaa
    14161 ggctcggttt acgcggcgat acattctttc agtttctacc tgacgagcta aaatagttga
    14221 tttaatatga taaagcgagg aataaaggat tacatccttt tagttttaaa ggggattgga
    14281 atgggggctg ccgatgtaat ccctggagtg tcgggtggta caatagcctt tattacaggt
    14341 atttacgaag aacttatcga ttcaattaaa agcgtagttg cgcctgaatc attaaagtac
    14401 cttaagtcat tctcactact tagctattta aaagccatta acgccgattt tcttttggct
    14461 gtaattggtg gtattgctat tagttttctc tcactggcca agttgatgca gtacctattg
    14521 accaatcatc ccatttttat atggtcgttc tttttcgggc tcattttggc atcggtttgg
    14581 tttgtggcca agattattga gaaatggaat gtttcaacat acttgttttt tattactggg
    14641 attattcttg gctttgccat tactaccata accccaaccg aaactcctaa tgatttgtgg
    14701 tttattttta tttgcggtgt cgtggctatt tgtgcaatga tcttacctgg aatttccgga
    14761 agttttattc ttcttcttat gggtaagtat atctacatga tgtcggcact cagcagtttt
    14821 aattttccaa taattttggt ttttttgttg ggtgctgcag ttggcatttt ggccttttca
    14881 aactttttat cgtggttact ccaccatttc catgctgcta ctattgctgt tcttgctggt
    14941 tttatgcttg gttcactaaa taaagtatgg ccatggaaag tggccaccca aacctttatt
    15001 gatgctcagg gtataattcg tccactgata gagaaaaatg ttttaccatc tacctatctt
    15061 attgaaaata aggccgagcc ataccttctt ggtgctatag ggtttgccat acttggtgca
    15121 ataattatta ttctatttga acgtttcttt tctaaacctg ttactaagga ggttgattta
    15181 agagagaaac ttggtttgta gagctaaaat tgttggttat aagggattgc tgttaccaaa
    15241 taaggtaaaa gcaatccttt tcatttttct cgtttcatgg ggtgtaaatg ctcaggaaaa
    15301 acccttggtg tatccgttcc gtgtgggtga ggtagttaaa tacaacgcct actacaactg
    15361 gcagtttgta tggctaaatg cagggactgc agaattttac ataaacgaca cattggttaa
    15421 tggagtaccg gcttaccatt tcaaatcgct aggagcttcg cttaatagtt acgactggtt
    15481 ttttaaggta cgggaaaggt ttgaatcaat agcaaaaaag gagaaccttg caccattatg
    15541 gtttatacgc gaaagctatg agggtggcta caaagttttc aaccggtata gctttagcta
    15601 tagcaactct aagctggtta ttgatagcta tacttccaac cgcccatata aacagaatac
    15661 ttttaccatt gataaaccca ttttcgatgt tctaactgcc atttattact gtagaactat
    15721 cgattttaaa agattgaata agggcgattt agttccgttg acaatggctg ttgatgatgg
    15781 agtttacaac ttgtatattc gttacctggg cccagaaaac ataaagcacc gcaatggcaa
    15841 ttcatatgac acctataagt ttagtgttaa actggttgag ggtactattt tcaagggtgg
    15901 tgaagatatg attgtatggg tttcgcgcga tgaaaaaaag attcccattg tagttgaagc
    15961 aaaaattttg attgggtctg ttatggcata tctacttgaa tataaggtta attgacatgc
    16021 tttttggcat tttttatgtt atatagatat tgcataaccc accccaacat ggtcagctat
    16081 gttgaggtgg gtttttagct ctagctgtac ttgtgaggtt cttgaaagcc ggtagcggat
    16141 tcctaccttc tggtagtata gcggtaaacg tgggtttttg ttgtaaaggt accatcccaa
    16201 atgcccatgt agcgataatc ggttgaaagg aatttgcagt cctgttttta tacccacctg
    16261 aaaatcgtca ttaacagggc tataatgctt tccacttagc tcgatgtgaa accgtgttga
    16321 tttgtcgaac ataatatcta atccagccat caaagggtag ctttgggcta gtggcattag
    16381 gtattccata gatattgata aggggtagta tttttccccg cctgcaggac cagtctcctt
    16441 gattcccgct aaaattacta ttcctagttg cgatttactg tctgaattct caaggtagac
    16501 ttctttttta caattatcta gtttatccct ttcagggtta taggtaaagc caaacgataa
    16561 cccaacaatg tttaatccat agttaggttt ttttattgct ccgtttgaga aatgattggt
    16621 ctcaatgcct gctgtaaggt tgaattgatt tgagaatttg aagctagatg tcagatttgc
    16681 tgttgcccag taattaatgg aggttgatat tgccacattg gttggattat gggaagggga
    16741 gtatttccgg gttatgtacg ccaaccctaa cccaaaatcg gttctaactg agaacttttt
    16801 aaattgaagt agttggtacg aaataaaggg ttgtattccc caggcatttc ccagaaccct
    16861 tttattccct aaatcaatac cggttaaagc aattccaaca taaggcattc catggcggtt
    16921 gtgccatgct tttctcccat tagttttctg tgatactgaa aatcgaaatc cttttacagt
    16981 gccatcagtt agaggcttaa gggtatcgct gtgtgctaat actttcccca taaaacctga
    17041 cgcggatgaa ctataaatgt ttgcccaatg ttcagcgttc tgagcatcta attcaaaacc
    17101 caaacaatgt agaattaaaa gggtgatata gtgaaatctt actctaaaca tatccaaaaa
    17161 taagtaacct acccgaataa tccgagtagg ttacaccaaa aatgtaaacc taaaacctaa
    17221 accaactaat gctcaatggc tacttttgat gagtttacaa acccatttga cgatattcgg
    17281 agaatgtata gccccctggg taattcattt ggaatattaa ttccgattgt attttctcct
    17341 gaaaagttat gcctcgtctc gctataaaca agctgaccgg ttaagttgta catatcaacc
    17401 tttacatggc tattgctggg ttggaagtat tttataaaga tatttccaga ggcaggatta
    17461 ggccaaacca ttatctctgc tttgcttgat ttgggttgat ttatgccaac gtatatatcg
    17521 ttgcgtttaa tcctttcaga agtaaacatg cggtattccg ctggattaag ggttaactgg
    17581 tagcttgttg ttgatagttg aacggaatcg ccactgtagt attcgtacca ccaacccgta
    17641 ttgttaaagg tgatagtagt actcttctgg ttaacatcaa aattaccagc aattaccaca
    17701 tccattgcac tactattaag ctttagccac ttaattgctc cggagaggtt ataatcgtag
    17761 ttatctgttt taaaaacctc atactgttgc ttaagcctgt ttaggtgggc aaaggtgttg
    17821 aatatctctc tgcgattatc gtcctgatag tattcccagt gtaatggttt tcggccaagg
    17881 cgaccattat agtctatgct tacatcgtaa ccaagttcac caaactgcca aatcatttta
    17941 ggtccgggaa atggaataaa taggtttgct gcggtttgca ttcgcattaa ggcaattggc
    18001 aatgttttca cgttatgatc tgagttgctc gcgttgccgt actgaaggtt tttgtacatc
    18061 agccgctctt catcatgact ttccatgtac cctataacat gaggcacgct ccaaccacgt
    18121 tgttttgctg ctacccacga tatatcggat gttgaaaccc aacccatagt agcctcgccg
    18181 taggcgtaat tcatgttacc ccatattagc atacccttcc cctcgctgct acgatactca
    18241 gcaagtgcca tttcttcgct gttatcacag aaatgttcaa ggatgatata agcgttgggg
    18301 ttaactgacc atattttgtc ggcaatgcgt ttcagaatcg caatacgtgt agcatcgtat
    18361 gccgatcctg cattgggagt attggtaaac ccttttgtga agtcaaaacg gaatccatcc
    18421 actttatact cggttagcca gtatgtgaga aaggaatcga caaacttttg agtttcggca
    18481 ctttcgtggt taaaatcgta tccccaac
//
LOCUS       DAOOWJ010000048        18491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3020_length_18491_cov_8.353385, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000048 DAOOWJ010000000
VERSION     DAOOWJ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 18491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18491
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3020_length_18491_cov_8.353385"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2186)
                     /locus_tag="PLW22_06320"
     CDS             complement(<1..2186)
                     /locus_tag="PLW22_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HOU74355.1"
                     /translation="MKFTKIILALALASISISGSSQDQVEWYRYPAISPNGKTIAFVY
                     KGDIYTVSASGGEAVALTANPAHDYLPVWSHNGDYIAFASNRYGNMDVFIVPSRGGEP
                     KRLTYHSADELPYAFTADDKHVLFGAARYDMASSRLYPTASQPELYSVPVNGGLVNQV
                     LPVPAEWVSVSGDGKLLAFHDKKGGENEFRKHHLSSIARDIWIYNTQTQEYKQLTNYK
                     GEDRNPVFAPGDSVIYFIREDSNFNVFKLSLSNSSEVKQVTNFTTHPVRFLSVSQNGT
                     LCFGYDGSIYTMGNNGISNKVDIKINRDFQQNPEQTVSLSGNIRELSVSPDGKEVAFI
                     ARGDVFVTSVEGAFTKRITQTPEQESFVAFTPDGKSLVYASEREGRWQIFKASRVRPE
                     EPFFFASTLIKEELLIGNEYDNYQPLPSPDGKKLAYIEDRTTLKVLDLESKSAKTLLT
                     SKELYYMSDGDQRFCWSPDSRWLLVEYSPNMSNNEIIALPADGSEPFRNLTQNGYDDF
                     TPRWSNGGKQVLWLSTRDGLRSYANSGMRQSDVYTLFLTRDSWDKFRLSKDEYNLLKA
                     IEDKQKEAKQQKVDDKKGKKDKDNKDKEKVDSSLKFDWEGFEYRSARLTTSSADIADA
                     LLSKDGETLYYLARYDKGYNLWSMNIREKEAKILVEMDVDYAKLYWDKDEKNMFMLAD
                     GKIYKVDVDKGKREALKIAAELTVNTDAERQAMYEHVVIRTRKGFYT"
     gene            2286..3455
                     /locus_tag="PLW22_06325"
     CDS             2286..3455
                     /locus_tag="PLW22_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOU74356.1"
                     /translation="MKIKLRKMFSLSNTEHVFEGRSNASLRCESLILSILSKKMVAKS
                     LILLSIFAKRLKIFRDFPGKLLVKRFFSHNSIDELKEISTQNFHNRILSCILPVSNGN
                     GSTVNLLSDLANDKYNIPGLEFLFLNVDDYLEPRILSLVSNDGFLNQVDKKAFQSFTD
                     YVDTLCEIASRNNKSIILYTANFAGFSAIHAIAIDLMQRYNTQKVTVFLLFHLCHLNS
                     QNRIVDLVRRGSSQGFTPGIAIAKYLTTEYVNKKFVDNSHNDSSCHGFNQTKRVFYEV
                     TKFLLNNIDHVSALLISHNPDNILYLTSLMEGCGVGYSNKNVTFLQRYGMAKSLTLNL
                     ANRGYNVATIIPHGNLTDTLRWMHRLYGFDPSLPNILTEQRFVILRELNRRKLEN"
     gene            3462..3941
                     /locus_tag="PLW22_06330"
     CDS             3462..3941
                     /locus_tag="PLW22_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74357.1"
                     /translation="MKFDNQDTVYGIHSLKLVLAVVVVIVIVLMYLTSIPDYIESSIG
                     ISQGWVIAIVVILYLMFFGYFIWMDAAFISYNDEGNKLVIRTFKLRPWGGKKISMEIP
                     HSEFYKYEITHKWPKKQLHIYVKKGNKIMKYPPVSVVSLTAEQYQKMESSLNQLSKA"
     gene            3945..5066
                     /gene="dprA"
                     /locus_tag="PLW22_06335"
     CDS             3945..5066
                     /gene="dprA"
                     /locus_tag="PLW22_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOU74358.1"
                     /translation="MGESELQYRIAIELIPKVGSITAKRLIAYCGSAEAVFRQSKKAL
                     LKIPGVGETIAAEVVNQNVLHLAEKEIEFIERYKIKALYFTDPEYPERLRQCEDGPVM
                     LYVKTHSSINFNSDKIISIVGTRKATAYGKQTCEAIVNGLAAKGHNPIIVSGLAYGID
                     IVAHKSALKNNLQTVAVLGHGLDTIYPVAHRAVAKEMVEQGALVTDFISGTPFDPNNF
                     LRRNRIIAGISDATIVVESALEGGALVTADIANSYNRDVFAVPGNVTSTYSMGCNMLI
                     KQNKAALVESADDIEFLLGWSTTSKTQTVKQYTINFDHFTNEEKKIINFLKEKGEETV
                     DLIALGTGLPVSTVLSSLLNLEFSGVVSSKPGKVFALKN"
     gene            5071..6420
                     /locus_tag="PLW22_06340"
     CDS             5071..6420
                     /locus_tag="PLW22_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOU74359.1"
                     /translation="MSKQPKPNRIMELYKKVQRFLLHGIWSMNLDELPPKLKWPFKYL
                     RVLLLALKGFFEDKVIVKASALTYYTLMSIVPIFAMAFGIAKGFGFERYLEQQLTTQF
                     KGQEEVINKVIVFANSLLARTGGGLIAGIGVVLLFWSVINVLSSIEHAFNDIWQVEKP
                     RSWVRKFTDYLSIMLISPILLIASGSIHIFLATTVTSIAEKHEHVSYISPFLIDMLQF
                     LPYLLIWILFSFIFIAIPNTKVSYKSGIVAGIIAGSGFVILQWLYITLQIGVSRYNAI
                     YGSFAALPLFLFWVQISWQIVLFGAEISFAYQNVDMYEFERETTHISHKNRKLLALLV
                     LSTIVKRFMNGEKPATNLELSVNLKIPQRLMRNLLDLMVNCELLNEVLISESKDIGYQ
                     PALHVEHLTIAFVEEKLDSYGFEIEPDIPELKQVKQVCTSFTERYRELTASTLIGSI"
     gene            complement(6422..6940)
                     /locus_tag="PLW22_06345"
     CDS             complement(6422..6940)
                     /locus_tag="PLW22_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018451909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU74360.1"
                     /translation="MNDTLRIILNRKSVRSFTGQEVSNELLEELARAGMAAPSARNLQ
                     PWAFVIVTNRATLDTLAERLPYAKMLFEAPAAIAVCGDLSKAGDSPEGYWVQDCSAAT
                     QNILLAAEAMGLGAVWTGVYPRPDRVAIVKEVLGLPDNVLPLNVIPIGFPKGEHQPKE
                     KFKVENIHWQNW"
     gene            7232..9181
                     /locus_tag="PLW22_06350"
     CDS             7232..9181
                     /locus_tag="PLW22_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU74361.1"
                     /translation="MTYSNSKKSISGIVAIATIVVVALVSSWMALSALMGNALAYQLN
                     LGLAIGSVPIKIDGLSAWFLLIINFTSITGAIYGLGYLKGYSNPAAKLNLHWIMFAIF
                     HLSMILVCIVQNGFVFLVIWEIMSLSSAMLVMFDGHNPKTLKAGINYLVQMHISIVFL
                     TVGFIWLYAVTGSFNFDSLQSFFIGNNNIWLFLVFFTGFGLKAGFIPLHSWLPHAHPS
                     APSHVSGVMSGVIVKMGIYGIIRVASYLTNNHLLLGEIVISISVLTGIYGILNAAAHR
                     DFKRMLAYCTIENIGIIGIGIGIGLIGISIKSDIIYFLGFGGALLHVLNHSLFKSLLF
                     YSAGSVYQQTHTRDMDKLGGLSKYMPKTAFLFLIGAVAIAGLPPFNGFVSEFIIYSGL
                     VNGFGTGDNGLTILLILSFAGLSIIGGLSLLTFAKTYGVIFTGTPRTSLDHKPHEVSN
                     LMIVPQLAIIAAMLSVAFFPSFYVNTINFIIKDFSNGLPINPAFSSDFSNTLKSIMVF
                     SLAVFAVILVVWGIRKISTKNKSILADETWGCGYTNPTPRIQYTGKSFSKQLGKIFNF
                     FLIEKKKYNELNSGEIFPHKRQYFSFYTDFFETRFINLITQRILFTANYFRFIQNGRI
                     QSYVLYGIVFILAIFILSALNLFSF"
     gene            9191..11218
                     /locus_tag="PLW22_06355"
     CDS             9191..11218
                     /locus_tag="PLW22_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU74362.1"
                     /translation="MEALKLFLTIPLIAFLAMPFMSIKWRGIVAYLAVILNAILSSYI
                     AINVLGGENYQFIFSGNAVTGEIPVRIDALSAWFILIINFTILTGGLYGIYYMREYLT
                     MRNSITLHVTAFILLHTALLSLCGLQNSIVFLLTWEIMALSAFVLVIFEHNKVSTIKA
                     GINFLIQSHVSIIFLLLGFVWVAAKTGSYDFLSISQFTHEHGNSVSLVLFIFLFIGFA
                     IKAGFVPFHTWLPYAHPAAPAHVSGIMSGVLIKIGIYGILRVILLQNTNLEVIGYIII
                     AFSAISGIYGVMLAILQHNLKRLLAYHSIENIGIIGLGIGIGCVGLGTQNLIVASLGF
                     AGALLHTLNHSLFKSQLFFSAGIVYQATHTVQIEKLGGLIKKMPQTAFLFLIAALAIS
                     GLPPFNGFISEFLIYSGLYNWLPSADSGIIITVIFSILALVLIGGLALLCFTKAFGIV
                     FLGNPRHQFKHQISEASFGQILPLYFITALIVAIGLFPNAFLTIVSKPLNLFIALDQN
                     NTSTSIGGNSEILQMISFSFALLLVFTAIIFGIRQMVQRKHAISADETWGCGYTGETK
                     KMQYTANSFARSYYKLFRFILLVFKKEKTVENVFPRHAEFESSPYDKIEKLLIDKPIQ
                     SIRFFMGKFTFLQNGRLQYYIIYGITFILAIIAIPLLYEKLLQLIEILKHL"
     gene            11221..12126
                     /locus_tag="PLW22_06360"
     CDS             11221..12126
                     /locus_tag="PLW22_06360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOU74363.1"
                     /translation="MLSAILIILAAIFYTGIINRTKSIASGRKGPGLFQHLSDVIRLF
                     KKGAVYSKTTSFIFQIAPTIYFSTVVIAMLLVPIGNSKGLFSFQGDFILFAYILALGK
                     FFSIIAALDTGSSFEGMGASREALFSLFAEPAFFILMGSLALLTGHTSFHEIFSALHL
                     GSYTTYAIAALGSFVLVMISMVENSRMPIDDPKTHLELTMIHEVMILDNSGFDLGLIL
                     IAGYLKFAIYAAIVFNLFVGTIHYEYSIPLFFFTQFILAVFMGLVESFMARFRMSHNP
                     QFIMALSSVALLIFFGVLLIIGKFI"
     gene            12149..12781
                     /locus_tag="PLW22_06365"
     CDS             12149..12781
                     /locus_tag="PLW22_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74364.1"
                     /translation="MINVLLITFLITLLYMSIANRLLTYLKVLAFQGVILFVVTFIQL
                     HEINTLNLILILLETIVFKSVAVPLFMAHILKRNKITREAEPYVPYFVSLIITTLIVV
                     TTILLSGTIKDPNLDKIFFVVALSTIFFGLYIIASRRKIITHVMGYLVIENGVFVLSL
                     AVGNEMPMLVNLGVMLDIFASVLILGIFLNKIGDVFKNIDVEELSNLKDY"
     gene            12801..14225
                     /locus_tag="PLW22_06370"
     CDS             12801..14225
                     /locus_tag="PLW22_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU74365.1"
                     /translation="MTILYLLGALSIGAGLFINRNKLVNYILTSLFIVLQWGFTIYAY
                     FHLNITEAEYFTFDSLGVLLLLVLSTISIPAIFHSHRYIDLHNEPAKSQSIYFSALII
                     LIAAIGLAYLANHIAVMWIFAEITTLAASALIYHHRNKLALEGTWKYVFICAVSVAFI
                     FIGILFLSLSLGKASSDSLSFQYMISRATTLNQFWLKLAFLFIFTGFTVKAGLVPMFT
                     AGVDAKDKAPGPIAALLSSVLMNMGVVGIFRTYKIIANTPIQYWANLVIGISAVLSIF
                     VATVYMIKVKNIKRMFAYSSIEHMGLVMLAISVGGIGYYAAILHIVLHAFVKSSLFFQ
                     YNQIYRVYQDKNIYHIGNYFKYNPTGAIVILLGFISATAMPPSGLFVSEFLIFRALFE
                     GHQIFLLIAVLLLLTIIIWAFAKNILKVLFTPLTNFDESNVPIIKPWESYSQYALIIL
                     SIYLGLTPPDFFVQLIKESVMFLS"
     gene            14231..15730
                     /locus_tag="PLW22_06375"
     CDS             14231..15730
                     /locus_tag="PLW22_06375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOU74366.1"
                     /translation="MNYISIKNNQTIPVADIPVLDYQQFSETNISLLSKHADRHCVNY
                     FGYIQGNSIKLVSCIADDTSHEIYISSCLVDSSKKLESMSSRLLCFEKFEREIHENFG
                     VVFENHPWMKPVRYPYNRADKSSVMDNYPFYSIDSEELHEVGVGPIHAGIIEPGHFRF
                     ICNGEQILHLEIQLGYQHRGLENLFLQKKKLLQRVTLAESIAGDTVAGHTTAFCYLWE
                     SLSGFDPKPEIDFYRTLALELERIAIHTGDLSAICTDIAYQLGSSVFGRLRTPIINFI
                     QEWGGNRLSKGLIRPGKTQFPFTKELGDRLNEILNDYEPDFNEMCKELFKLPSALSRL
                     ERTGTVSYDDVLNIGAVGMSARMSGLLRDIRTTHPYALYPQLNHKPILKHHGDVYSRA
                     QIRREEIWQSMAYIRQLLKDIPESKANYEMPIKLKPNAFSISLVEGWRGEICHCAITD
                     ADGDLAVYKVKDPSFHNWLALALAVRNNEISDFPICNKSFNLSYCGHDL"
     gene            15742..16494
                     /locus_tag="PLW22_06380"
     CDS             15742..16494
                     /locus_tag="PLW22_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOU74367.1"
                     /translation="MLDNLKILYHQGKQFIPDVRTAKVPGIFRGRPIINSHKVNVTEL
                     VDLCPMNAISSNPVSIDLGKCTFCGECALRFPEKIKFTTDYKISTNERERLIVKEGDD
                     FAIEVNPVTVRKEIRKIFGKSLKLRQVSAGGDNSCEWELNAANNVQFDMSRFGIEFVA
                     SPRHADGIAITGPITQNMAKALEIAYNATPDPKVIVLIGTDAISGGIFADSPALDRSF
                     LENHRVDLYIPGNPAHPLTIINGLLDLTRKKK"
     gene            complement(16502..17542)
                     /gene="rlmN"
                     /locus_tag="PLW22_06385"
     CDS             complement(16502..17542)
                     /gene="rlmN"
                     /locus_tag="PLW22_06385"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOU74368.1"
                     /translation="MQKGETLVGKDLKQVEAWLSELGFPTHYGVKLTGWIYRKRIQNY
                     ADINDIPKHILKALKDEFGEVRGHIGYERYPGTDGSVKYLFTNAQGQHYESIFMDNGK
                     RKTLCVSTQAGCRMGCRFCMTGSIGFKGNLDAGSIVGQLLAIPESKSVNRLVIMGMGE
                     PFDNWVEVSQALEILTSAWGVAFGRSNITLSTVGILDGLERFLMNPPCNLAISLHSPF
                     PYERALLMPSEVKNPISRVVDILLKHPIPKPLRLSFEYVALGGINLSEQHAKAVCELL
                     KGLNYHINIIPWNEHSAASEFRKPTPVELENFTRWLNQHGALWTIRQSRGNEVGAACG
                     QMAGKRIDLKRI"
     gene            17953..>18491
                     /locus_tag="PLW22_06390"
     CDS             17953..>18491
                     /locus_tag="PLW22_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU74369.1"
                     /translation="MSKLQVKLNNIYQFVDASDVLSLKNKVNDCQKLIEGKTGKGNDF
                     LGWVTLPAEITDDQLKSIEKVANELRSKSEFVVVVGIGGSYLGAKAVIEALGNSFSML
                     DNGGTKVLFAGNSICEDYHAELLKLLDKHTYSIVVISKSGTTTEPAIAFRLLKQHLEG
                     KVGKTEAASRIVAITDQSKG"
BASE COUNT         5325 a         3693 c         3766 g         5707 t
ORIGIN      
        1 gtgtaaaatc cttttcgggt tctgataacc acatgttcat acatggcttg cctttcggca
       61 tcggtgttaa ctgtaagctc tgctgctatt tttaatgctt cccgtttacc cttgtcaaca
      121 tcaaccttat agattttgcc atcggcaagc atgaacatgt ttttttcgtc cttatcccag
      181 taaagcttag cataatcaac atccatctca acaaggattt ttgcctcttt ctcacggatg
      241 ttcatcgacc aaaggttgta acctttatcg tagcgggcaa ggtagtaaag ggtttcgcca
      301 tccttgctca gtagggcatc ggcaatatcg gcagaggatg tggttagacg tgctgaacga
      361 tactcaaagc cttcccagtc gaacttcaat gagctgtcaa ccttttcttt atctttatta
      421 tccttatcct ttttaccctt tttatcgtca accttttgtt gcttagcttc tttctgttta
      481 tcctcaattg ctttcagcaa gttgtactcg tccttactaa gcctgaattt atcccatgag
      541 tcgcgggtaa ggaaaagtgt atatacatcg ctttgacgca taccgctatt ggcgtagctc
      601 ctaaggccat cgcgagttga aagccaaaga acctgcttgc ctccatttga ccagcgaggg
      661 gtaaaatcgt cgtaaccatt ttgtgtaaga ttcctgaaag gttcagagcc atcggcgggt
      721 aaggcaatta tctcgttgtt ggacatgttt ggtgagtatt caaccaggag ccagcggcta
      781 tcggggctcc agcaaaagcg ttggtcgcca tcgctcatgt agtatagttc ctttgatgtg
      841 agtaatgttt ttgctgattt gctttccaaa tctaaaacct taagggttgt tctatcctca
      901 atgtatgcta actttttgcc gtcgggtgag ggtaggggtt ggtagttgtc gtattcgttg
      961 cctatcagta gttcctcttt aattagggtt gatgcaaaaa agaagggttc ctctgggcga
     1021 acacgcgaag ctttaaatat ttgccagcgg ccctcccgct cgcttgcata taccaggctt
     1081 ttgccatcgg gagtaaaggc tacaaagctt tcctgttcag gggtttgggt tattcgtttt
     1141 gtaaatgccc cctctaccga ggtaacaaat acatcgcctc gtgcaatgaa tgcaacctct
     1201 ttgccatcgg gcgacacgga aagttcgcgt atgtttccac taagcgatac tgtttgctca
     1261 gggttctgct ggaagtcgcg gtttatcttt atatctactt tattgctaat tccattatta
     1321 cccatagtgt atatggaacc atcgtagcca aagcaaagtg ttccgttttg gctgacgctt
     1381 aaaaaacgaa ccggatgggt ggtaaagttg gtaacctgct taacctccga tgagttggat
     1441 agtgaaagct tgaatacatt aaaattacta tcctcacgga taaagtaaat tactgaatcg
     1501 ccaggagcaa aaaccggatt acgatcctca cccttatagt ttgtgagctg tttgtattcc
     1561 tgggtttggg tgttgtatat ccagatatcg cgtgcaatag aagaaaggtg gtgcttgcga
     1621 aactcattct caccgccttt tttatcgtga aatgctagaa gctttccatc gcctgagacc
     1681 gatacccatt ctgcgggaac gggtagaacc tgattaacta aacccccgtt gaccggaacg
     1741 gaataaagtt caggttgcga tgcggttggg tataggcggc tggaggccat gtcgtatcgt
     1801 gcggcaccga atagcacatg cttgtcgtcg gctgtaaagg cgtagggtaa ctcatcggct
     1861 gaatggtagg ttaaacgttt gggttcacca cccctggatg gtacaataaa cacatccata
     1921 ttcccatacc tattcgaggc aaaggcaatg taatcgccgt tgtggctcca taccggtagg
     1981 taatcgtgtg ccggattagc tgtcaatgca actgcctcgc ctccgctggc agaaaccgtg
     2041 taaatatcac ccttgtaaac aaatgcaatg gtttttccat taggcgaaat ggctgggtaa
     2101 cgataccatt ctacttggtc ttgggaactc cctgatattg atatgcttgc aagagctagg
     2161 gcgagaatta ttttagtgaa tttcattttg gtatgtttta aaagttcgtt tagtcatact
     2221 ctaaattaaa ccgcttatcc agttaatggt ttaaaatgtt tagttttatt taaaaattga
     2281 taattttgaa gataaaatta aggaaaatgt tctcgctcag taacactgaa catgtttttg
     2341 aaggaagaag caatgcctcg ctccgctgcg agtccttaat tctttccatt ctttccaaaa
     2401 aaatggtagc aaagtcccta attttactct caatttttgc caaaaggtta aaaattttta
     2461 gagattttcc aggtaaactt ttagtaaaac gattttttag ccacaattcc atagatgagc
     2521 taaaagaaat atctacccaa aattttcaca atagaattct ctcctgtatt ttaccagttt
     2581 caaacggaaa tggttcaact gtcaatttgt tatcggattt agctaacgat aaatataata
     2641 ttccggggtt ggaattcctt tttcttaatg ttgacgatta cctagaaccc cgcattctta
     2701 gcctggtatc aaacgatggt tttttgaatc aggtcgataa gaaagctttt caatcgttta
     2761 ctgattatgt tgatacccta tgcgaaattg ccagtagaaa taataaatcg ataatcctat
     2821 atacagccaa ttttgcaggt ttttcagcaa ttcatgcaat agccattgac ttaatgcaac
     2881 ggtataacac ccaaaaagta acggtatttt tacttttcca tttgtgccat cttaactcac
     2941 aaaacagaat agttgacctt gtaagaaggg gctcaagcca aggttttacc cctggtattg
     3001 caattgccaa gtatttaacc acagagtatg tgaataaaaa gtttgtcgat aatagccaca
     3061 acgactctag ctgtcatggt tttaatcaga ccaagcgagt gttttatgag gtaactaaat
     3121 ttctgctaaa taacatcgat catgtttcag cattactgat tagccacaac cccgataata
     3181 tactttacct tacatcgctt atggagggat gtggtgtagg gtatagcaat aagaatgtta
     3241 cctttttgca acgttacgga atggcaaaat ccttaacatt aaaccttgcc aacaggggat
     3301 ataatgttgc aacaattata ccccacggta acttaactga tacattacga tggatgcata
     3361 ggctttatgg cttcgacccg tcgttgccaa atattcttac cgagcagcgt tttgtcatac
     3421 taagagagct aaataggcgc aaattagaaa attgatgcat tatgaaattt gataaccagg
     3481 acactgtata tggaatccac tccctgaagc ttgttttagc agtagtggtg gtaattgtta
     3541 tagtattaat gtacttaaca tcaattcccg attatattga gagtagtatt ggaattagcc
     3601 agggatgggt gatagccatt gttgtaatac tttacctgat gtttttcggg tattttattt
     3661 ggatggatgc tgcatttatc tcgtataacg atgagggcaa caagctggtt attcgtactt
     3721 tcaagctacg tccttggggt ggtaaaaaga tatcgatgga aataccacac tcagaattct
     3781 acaagtatga gattacccac aaatggccaa aaaaacagct acacatttat gtaaaaaagg
     3841 ggaataagat catgaaatac cctcccgttt cggttgtgtc gttaacagca gagcaatacc
     3901 agaaaatgga gagttccctt aaccaactat cgaaagcata atgcatgggc gaaagcgagt
     3961 tgcaatatcg catagcaatt gagcttatcc caaaggtagg gagtataact gctaaacgtt
     4021 taattgccta ctgcggaagt gccgaggcgg tttttcgtca atccaaaaaa gcgctgctta
     4081 aaatacctgg cgttggcgaa accattgcag ctgaagtggt caaccagaat gtgttacacc
     4141 ttgccgagaa agagattgag tttattgaga ggtataaaat taaagcccta tactttactg
     4201 accctgaata tcccgaaagg ctgcgccagt gtgaggacgg cccagtaatg ctttacgtga
     4261 aaactcattc cagcataaac tttaattctg ataagataat cagcatagtg ggaacccgaa
     4321 aggcaacggc ttacgggaag caaacatgcg aagccattgt aaatggactt gccgccaagg
     4381 gtcataaccc aataatcgtt agcggattag cttacgggat tgatatcgta gctcacaaaa
     4441 gcgcattgaa aaataacctg caaacagttg cagtgctagg acatggtctg gataccattt
     4501 atcctgtagc acacagggcg gtagctaagg agatggttga gcagggagct ctggtaaccg
     4561 attttatttc tggaacgcca tttgacccca ataactttct gaggcgaaat aggattattg
     4621 ccggcatatc cgatgcaacc attgtggttg aaagcgccct ggagggtggt gcgcttgtaa
     4681 cagccgatat tgcaaactca tacaatcgcg atgtatttgc cgtgcccggc aatgtaacat
     4741 caacatactc catgggttgt aacatgttaa taaaacagaa caaggcagca ctggtggagt
     4801 cggccgacga tattgagttc ctgctgggtt ggagcactac cagtaaaacc caaaccgtta
     4861 agcagtacac aataaacttt gatcatttta ccaacgagga gaaaaaaatt attaactttc
     4921 tgaaggaaaa aggggaagaa accgttgacc tgattgccct tggaacagga cttccggttt
     4981 caaccgtttt atcctcgctt cttaatttgg agttttcggg agtagtaagt agcaaaccag
     5041 gcaaagtatt cgccctaaaa aactaatgca atgagcaaac aaccaaaacc aaacaggata
     5101 atggagctgt ataaaaaggt tcagcgtttc ctgcttcatg gtatatggag catgaacctt
     5161 gatgagcttc cgcctaagct gaaatggcca tttaagtatt tacgggtgct cttgcttgcg
     5221 ctcaagggtt tctttgagga taaggtaatt gttaaggctt cggctttaac atattacaca
     5281 ctaatgtcca ttgttccaat ttttgcaatg gcatttggaa ttgcaaaggg gtttgggttt
     5341 gaacgatacc ttgaacagca gttaactacc cagtttaagg gacaggaaga ggtgattaat
     5401 aaggttattg tgtttgcaaa ctctctacta gcacgaaccg ggggcggctt aattgctgga
     5461 attggtgttg tactcctttt ttggtcagtt atcaacgtgc tatccagcat tgagcatgcc
     5521 tttaacgata tctggcaggt agaaaaacct cgcagctggg tacgcaagtt taccgattat
     5581 ctttccatta tgctcatttc gccgattcta cttattgctt ccgggagtat tcatattttc
     5641 cttgctacta cagtgacaag cattgctgaa aagcatgagc atgtgagcta catcagccca
     5701 ttcctgattg atatgctcca gtttttgccg tatctattaa tttggatact tttcagtttt
     5761 atttttatag ctattcctaa cacaaaggtt tcctacaaat ccggtattgt tgcaggaatt
     5821 attgctggtt ctggttttgt aattctccag tggttgtata tcacccttca aattggggtt
     5881 tcccgatata acgcgattta cgggagtttt gcagctttac ccttattcct tttctgggtt
     5941 caaataagct ggcaaatcgt gttatttggt gctgaaatat cttttgcata ccaaaatgtc
     6001 gatatgtatg aatttgaaag ggaaacaacc catataagcc ataaaaatcg gaagttgtta
     6061 gccttgctgg tgctaagcac cattgttaaa cgatttatga atggagagaa accggctaca
     6121 aaccttgaac tatctgttaa tctaaaaata ccccaacgac tcatgcgcaa cctcctcgac
     6181 ttgatggtta actgtgagct gctaaacgag gtgttaatta gcgagagtaa agatattggt
     6241 tatcagcctg cacttcatgt tgaacaccta acaattgctt ttgttgagga aaaactcgat
     6301 agctatgggt ttgagattga acccgatatc ccagagttga agcaggtaaa acaggtttgc
     6361 acaagtttta ccgagcgtta ccgcgaattg accgctagca ctttgatagg tagcatttag
     6421 tctaccagtt ttgccagtgg atgttttcta ctttaaactt ttccttgggt tgatgctccc
     6481 ctttaggaaa accaattggt ataacgttga ggggtaaaac gttatcgggg agtcctaaaa
     6541 cttcctttac aattgcaacc ctatcggggc gggggtaaac gcctgtccat acagccccca
     6601 aacccattgc ctcagcggca agcagtatat tttgcgtagc agcagagcag tcctgaaccc
     6661 agtatccttc agggctatcg cctgctttgg aaaggtcgcc gcaaacagct attgcggcag
     6721 gtgcctcgaa aagcattttg gcgtagggta aacgctcagc aagagtatcc agggtggccc
     6781 ggttagtaac aataacaaat gcccatggtt gaaggtttct tgcggagggt gcagccatac
     6841 ccgcacgggc aagttcttca agtagttcat tgcttacttc ctggccagtg aagctgcgca
     6901 cgcttttgcg gttaagaata attctaagtg tatcattcat ctccataata tttatcattt
     6961 atgctagaag atctatttta tgaaagccta aatttatata aaactaagtt aaataaaatg
     7021 aattatggga aataacgtat ttcattattt cgttactttg caccctgaat gaagaattac
     7081 taataatagg tgcaaatctg ctttttaaga attacatagt gttaaaattc tcattcgata
     7141 gtatagattt gaaatgtaaa cattgggggg aacctatatc accaagggaa agttatagta
     7201 tcttttttta ttctgaattt ggtaacttgt tatgacttat agcaatagca aaaaaagcat
     7261 tagtggaata gtagcaattg ctacaatagt agttgttgca ttggttagca gttggatggc
     7321 tttgtcagca ctgatgggaa atgcactggc gtaccagtta aatttagggc tagcaatagg
     7381 atcagtccca ataaaaatag atgggctttc cgcttggttt ctgttaatca tcaactttac
     7441 ctcaataacc ggggcgatct atgggctcgg atacctaaaa ggatactcaa accccgcagc
     7501 aaagttgaat ttgcactgga taatgtttgc catattccat ctttccatga ttttggtttg
     7561 cattgtacag aatggatttg tattccttgt gatttgggaa ataatgtcgt tgtcttcggc
     7621 aatgctagtt atgttcgatg ggcataatcc caaaacgctt aaggctggca taaactacct
     7681 tgtgcaaatg cacatcagca tagtattttt aaccgttggt tttatctggt tatacgctgt
     7741 tactggttca tttaatttcg attcgttaca atcattcttc atcggcaata ataacatatg
     7801 gttattccta gtattcttca ctggctttgg acttaaggct ggatttattc cactacatag
     7861 ctggctacca catgctcacc cctctgctcc ttcgcatgta tcgggcgtaa tgtctggtgt
     7921 gatagttaaa atgggtattt acggtataat cagggttgcc tcctacctca caaacaatca
     7981 tctactttta ggtgaaatag ttatttcaat ctccgtactc actggtatct atggaattct
     8041 aaatgccgct gcacaccggg attttaaacg catgcttgcg tactgtacaa tcgagaatat
     8101 cggaataatt ggaattggca ttggaattgg cctcattggc attagtataa aatctgacat
     8161 aatatacttt ttaggttttg gaggagctct gctccatgtg ctgaaccatt ccttgtttaa
     8221 atcgctgcta ttctactccg cgggctcggt ttaccagcaa acgcacacaa gggatatgga
     8281 taagttgggc ggactgtcaa agtatatgcc caaaactgca tttctatttt taattggagc
     8341 agttgcaatt gcaggacttc cacctttcaa tggttttgta tcggaattta taatttacag
     8401 cggattggtg aatggctttg gcactggcga taatggatta acaattctac ttatcctatc
     8461 ctttgctgga ctgagcataa ttggaggttt gtcgctattg acattcgcaa aaacttatgg
     8521 cgtgatattc actgggacac ctagaactag tctcgatcat aaaccccacg aagtatcaaa
     8581 cctaatgata gttccgcaat tggcaattat tgcggccatg ctaagcgttg catttttccc
     8641 atcattctat gtaaacacaa taaacttcat aattaaggat ttctcaaatg gattaccaat
     8701 aaacccagca ttttcaagtg atttctcgaa cacgctgaaa tctataatgg tattttcgct
     8761 cgcagtcttt gccgtgatac ttgtagtatg gggcatcagg aaaatatcaa caaaaaacaa
     8821 atccatttta gcagacgaaa catggggatg cggatataca aatccaacgc ctcgcataca
     8881 gtataccgga aagtcgttct cgaaacagtt gggaaaaata ttcaacttct ttttgattga
     8941 aaagaaaaag tataacgaac taaatagcgg ggaaatcttc ccgcataaaa ggcagtattt
     9001 ctcgttctac accgatttct tcgaaactcg ttttataaat ctgataactc agcggattct
     9061 tttcaccgca aattacttca gatttattca gaatgggcga atacagtcgt acgtgctgta
     9121 tggaatagta ttcatattgg ccatttttat tctttcagca ctaaacctat tctcatttta
     9181 aagtgtattg atggaagcat taaaattatt tctaactata ccgctaattg cctttctggc
     9241 aatgccattt atgagtataa aatggcgagg aatagttgca taccttgcag taatacttaa
     9301 tgctattcta tcatcgtata tcgctataaa cgttttaggt ggagaaaact accaattcat
     9361 attctcaggc aacgcagtaa caggcgaaat tccagttaga attgatgcgc tctcggcatg
     9421 gtttatatta ataatcaatt tcaccatttt aaccggaggc ctgtacggaa tttactacat
     9481 gagagagtat ttaaccatgc gcaacagcat caccctacac gttactgcgt tcatcttgct
     9541 acatactgcc ttgctgagcc tttgcggatt gcaaaattcg atagtatttc tactaacatg
     9601 ggaaataatg gctctcagcg catttgtgct ggttatattt gagcacaaca aggtttccac
     9661 aataaaagct ggaattaatt tcttgataca atcacatgta tcgataatat ttttgctact
     9721 cggatttgtt tgggttgcag caaaaactgg cagttacgac ttcttatcca tatcgcaatt
     9781 tacacatgag catgggaact cggtttctct ggttctgttc atattccttt ttatcggatt
     9841 tgccattaag gctggctttg ttccatttca cacatggctg ccctatgcac atcctgcagc
     9901 tccagcccat gtttcgggaa taatgtcagg ggtactgata aaaataggta tatacggaat
     9961 actccgagtt attctattgc agaataccaa ccttgaggta attggatata taattattgc
    10021 attttcggca atatcgggaa tatatggagt tatgctggcc atcctgcaac acaatttaaa
    10081 acggttattg gcataccata gcatagagaa tattggaata ataggactag gaattggaat
    10141 tggatgtgtt gggcttggca cccagaattt aatcgttgca tcgcttggtt ttgcaggtgc
    10201 actccttcac acgcttaacc actcgctatt taagtctcaa cttttctttt cggctggcat
    10261 tgtttaccaa gcaacccata ctgttcaaat cgaaaagctt ggtggcttaa tcaagaaaat
    10321 gccacaaaca gcattcctat tccttattgc tgcacttgca atatcgggac ttccaccatt
    10381 taacggtttt atatctgaat ttttaatata cagtggattg tataattggc ttccaagtgc
    10441 agacagcgga ataataatta cagtaatttt ttcgattctt gcacttgttc tgatcggcgg
    10501 actggcttta ctttgcttta ccaaagcatt tgggatagtt ttccttggaa atcctcggca
    10561 tcaatttaaa catcaaattt ccgaggcatc gtttggtcaa attcttccac tttactttat
    10621 caccgcacta attgttgcaa ttgggctatt ccctaatgcg tttttaacaa tagttagtaa
    10681 gccgttgaac cttttcatcg cactagatca aaacaatacc agcacaagca taggaggtaa
    10741 ttctgagata ctccagatga tatcattttc atttgcattg ttgcttgtat ttactgctat
    10801 aatattcggt attagacaaa tggtacaacg caaacatgct atttctgctg atgaaacatg
    10861 gggctgtggc tataccggtg aaacaaaaaa aatgcagtac actgcaaact cctttgctcg
    10921 gtcatattac aaactattcc gatttatcct tttagttttc aaaaaagaaa aaactgtgga
    10981 aaacgttttt ccaaggcatg ctgaatttga atcgagccca tacgataaga ttgaaaagtt
    11041 actgattgac aaacccattc aatcaatacg gtttttcatg ggtaagttta cctttctgca
    11101 gaatggaagg ctgcagtact atatcattta tgggataacg tttattcttg caataattgc
    11161 tattccatta ctatacgaga aactgttaca acttattgaa atactcaaac atttataatc
    11221 atgttaagtg cgatactgat aattttagcg gcaatattct atacgggtat aattaacagg
    11281 acaaaaagta tagcatcggg tcgtaaagga cctggactat ttcagcacct aagcgatgtc
    11341 attagattat ttaagaaagg ggcagtttac agcaaaacca ccagttttat ttttcaaatt
    11401 gctccaacca tctatttctc taccgtagta attgctatgc tacttgtgcc gattggtaat
    11461 tctaaaggat tatttagctt tcagggggat tttattcttt ttgcctacat actggcttta
    11521 ggtaaattct tcagtataat tgcagcgttg gataccggta gcagttttga gggaatgggg
    11581 gcaagccgcg aagcattatt ctcactattc gcagagcctg cattttttat ccttatgggc
    11641 tccttagctt tgcttactgg acatacctct ttccatgaga ttttttcagc actgcactta
    11701 ggttcttaca ctacttacgc tattgcagca ttaggttctt ttgtacttgt aatgatttca
    11761 atggttgaga atagtcgcat gcctattgac gatccaaaga ctcaccttga gttaaccatg
    11821 attcatgagg ttatgatact agacaatagc ggttttgatt tagggttaat tcttattgct
    11881 ggatatttga agtttgccat atatgcagca attgttttca acctctttgt tggaaccatc
    11941 cattacgagt attctattcc attgttcttt ttcacccagt ttattttagc tgtgtttatg
    12001 gggctagtgg aatcgtttat ggcacgtttc agaatgagcc ataacccaca gtttattatg
    12061 gcactttcat cggtggcact gcttattttc tttggcgtgt tacttataat tggaaaattc
    12121 atttaaacca gtaataaaag ttaacgcaat gataaacgta ctgctaataa catttctgat
    12181 aacgctgctt tatatgtcaa ttgctaatcg gctactgaca tatctgaaag tgttagcatt
    12241 ccaaggggtg attttgtttg tcgtgacatt tatccagctg cacgaaatca ataccctcaa
    12301 cctaatattg attttgcttg agactatagt ttttaagtcg gttgcagttc cgttatttat
    12361 ggcacacatc ctaaaaagaa acaaaataac acgcgaggca gagccatacg ttccatactt
    12421 tgtttcatta attatcacaa cattgattgt ggtaactaca atccttctat ctggaaccat
    12481 taaggatccc aacctcgata agatattctt tgttgttgct ctctccacaa ttttttttgg
    12541 gctatacatc atcgcttcgc gcaggaagat catcacacat gttatgggtt accttgtaat
    12601 agaaaatggt gtttttgttt tatctctagc cgtagggaac gaaatgccaa tgcttgtaaa
    12661 ccttggtgta atgctcgata tttttgccag tgtcctgatt ctgggcattt tcttaaataa
    12721 aattggtgat gttttcaaaa acatcgatgt tgaagaatta agtaatctta aggactatta
    12781 attaacctaa actcatgata atgactattc tatacttatt aggggcttta tcaataggag
    12841 caggactatt tatcaacagg aataagttgg tcaattatat tcttacctca ttattcattg
    12901 ttttgcaatg gggttttaca atttatgcat atttccattt aaatataact gaggccgaat
    12961 actttacttt tgattcgctt ggagtcctac tattgcttgt tttgagtaca atttcaattc
    13021 ctgcaatatt tcacagccat cgttacattg acctacacaa tgaacctgcc aaatcgcaat
    13081 caatttattt ttccgcgctg ataattttaa ttgcagcaat aggacttgca tatcttgcaa
    13141 accatattgc agtgatgtgg atttttgccg aaattactac acttgcagca tcggcattaa
    13201 tttatcatca ccgtaataag cttgcccttg agggtacctg gaagtatgta tttatatgtg
    13261 cggttagtgt tgcgttcata tttataggaa ttctttttct aagtctttca ttggggaagg
    13321 ctagctctga cagcctttca tttcaataca tgatatcccg tgccacaacc cttaatcaat
    13381 tttggctaaa gctggcattc ctatttattt ttacgggctt tacagtcaag gcaggccttg
    13441 ttcctatgtt tactgcaggt gtagatgcaa aagataaagc cccagggcct attgcagcac
    13501 tattatcgag cgtacttatg aatatgggtg tagttggcat ttttagaact tacaaaataa
    13561 ttgctaatac acctatacaa tattgggcca accttgtgat aggaatttca gctgtcttat
    13621 caatttttgt ggctactgtt tatatgataa aggtgaaaaa tataaagcgc atgtttgctt
    13681 actcaagcat tgaacacatg ggattggtta tgcttgctat atcggtaggt ggaatagggt
    13741 actatgctgc aattttacac atcgtgcttc atgcttttgt taaatcaagc cttttctttc
    13801 agtacaacca aatttacaga gtatatcaag ataaaaatat ttaccatata ggtaattact
    13861 ttaaatacaa tcctactggg gctattgtca ttttgcttgg attcatcagt gcaacggcaa
    13921 tgccaccttc gggtttattt gtgagcgaat tccttatctt cagagcatta ttcgaagggc
    13981 accaaatatt cctactcatt gctgtgctat tattgttaac aataataatt tgggcttttg
    14041 caaaaaatat actcaaggtg ctttttacac ctttgactaa tttcgacgaa agcaatgtcc
    14101 ctataataaa accatgggaa tcctactcgc agtatgcatt aatcatttta tcgatatacc
    14161 ttgggttaac cccacccgat ttttttgttc aactaattaa agagagtgta atgttcttat
    14221 cataaaagac atgaactata tatcaataaa aaataaccaa actattccag tggctgatat
    14281 tccagtgttg gattatcaac aattcagtga aacaaatatt tcgttgcttt ctaagcatgc
    14341 cgatcgacat tgcgtaaact attttgggta catccaaggc aatagtatca aacttgtaag
    14401 ctgcattgcc gatgacacaa gccatgaaat ttatataagc tcgtgcctgg tggactcatc
    14461 caaaaaatta gaatcgatgt cgagcaggct gctttgcttc gagaagtttg agcgggaaat
    14521 acacgaaaat tttggagtag ttttcgagaa ccacccctgg atgaaaccgg ttcgataccc
    14581 ctacaataga gctgacaagt caagtgttat ggataattac ccattttact ccattgacag
    14641 cgaagaactt cacgaggtag gggtagggcc aatccatgcg ggcataattg aacccggtca
    14701 tttccggttt atttgcaacg gagagcaaat tctccatctg gaaattcaac ttgggtatca
    14761 acatagaggg ttagagaatc ttttcctgca aaaaaagaaa ctgcttcaac gggtaactct
    14821 tgccgaaagc attgctggtg atactgttgc tggccataca actgcttttt gctacctttg
    14881 ggaaagttta agtggttttg acccaaaacc tgaaatcgac ttttacagaa ccctagcatt
    14941 agaacttgaa cgaattgcca tacatactgg cgatttaagc gccatttgca ccgatattgc
    15001 ctaccagttg ggtagttctg tttttggccg attaagaact ccaattataa actttattca
    15061 ggaatggggg gggaacaggc tttcaaaagg tttgatacga ccaggaaaaa cacaatttcc
    15121 atttacaaag gaacttggcg ataggctcaa tgagatatta aatgattatg agcctgactt
    15181 caatgagatg tgcaaggaac tatttaaact gccaagtgct ctgtcaagac ttgaacgtac
    15241 cggcacagtt tcctatgatg atgttctgaa cattggggca gtaggaatgt cggcacgcat
    15301 gagtggcctc ctaagagata tcagaaccac tcatccatac gctttatatc cacagctaaa
    15361 ccataagcca atcctaaaac accatggtga tgtttactca agggcacaaa ttcgcaggga
    15421 agagatatgg caatccatgg cctatattcg tcagttgcta aaggatattc cggaaagcaa
    15481 ggccaactat gaaatgccca ttaaacttaa accaaatgca ttctcaatat cattggtaga
    15541 gggctggagg ggtgagatct gccattgtgc cattacggat gccgatggag atcttgccgt
    15601 ttacaaggtg aaagatccct cattccataa ctggcttgcg cttgcacttg cggttcgtaa
    15661 taacgaaatc tctgatttcc cgatttgtaa caagagtttt aacctatcat attgcggaca
    15721 cgacctataa aactatttgc catgctagac aatttaaaaa ttctatacca ccaaggtaaa
    15781 caattcatac ccgatgttag aacagcaaag gttcccggaa tatttagggg aagacccatc
    15841 attaactcac ataaggtaaa cgtaacggaa ttagttgacc tttgccctat gaatgcgatt
    15901 agcagcaatc ctgtttcaat cgacttgggc aagtgtacct tttgtgggga atgcgcacta
    15961 agatttcccg aaaaaattaa gttcactacg gattataaaa tttcaactaa cgaacgggag
    16021 agactaatag taaaggaagg cgatgatttc gcaatagaag ttaaccccgt aacagttcgc
    16081 aaagagatcc gaaaaatctt tgggaaatcg ttaaaactta ggcaggttag cgctggtggc
    16141 gataatagtt gcgagtggga gttaaatgcg gccaacaacg ttcagttcga catgagccgt
    16201 ttcggaattg agtttgttgc ctcgccccgc catgccgatg gcatagcaat aacaggtcca
    16261 atcacccaga acatggcaaa agccctagag attgcttaca acgcaactcc cgatcctaaa
    16321 gtcattgtcc taattggtac tgatgccata agtggtggta tttttgctga tagcccagcc
    16381 ttggatagaa gtttcctgga gaaccacagg gttgacctgt acataccagg aaacccagca
    16441 catccgctta caattattaa tgggctattg gatttaacaa ggaaaaagaa atagcaatcg
    16501 tttaaatacg ttttagatca atgcgtttgc cggccatttg gccacaggcc gccccaacct
    16561 cgtttcccct tgattgacgt atagtccaaa gcgctccatg ctggtttaac cagcgagtaa
    16621 agttttccaa ctccacaggg gttggtttcc tgaattcgct agcagcagag tgctcgttcc
    16681 agggaattat attgatatgg taattcagcc ctttcagcag ttcacacaca gctttggcgt
    16741 gctgttcact caggttaatt cctcctagag caacatactc aaacgagagc cgtaaaggct
    16801 ttggtatggg gtgtttgagt agtatgtcaa ccactcggct aatcggattt tttacttccg
    16861 atggcatcag caacgccctt tcatagggaa agggcgaatg aaggctaatg gccagattgc
    16921 atggcgggtt catcaagaac ctctctaaac catcaaggat accaacggtt gagagcgtaa
    16981 tgtttgacct tccaaaggca accccccagg ccgatgttaa aatctccagc gcttggctaa
    17041 cttcaaccca gttatcgaaa ggctcaccca tgcccatgat aaccaatcgg tttacacttt
    17101 tactttcggg gattgctaac agctgtccta caatactgcc ggcatccaaa ttacctttga
    17161 aacctatact cccggtcata cagaacctac atcccatgcg acacccggcc tgagttgaca
    17221 cgcaaagcgt tttacgcttg ccattgtcca tgaaaattga ttcgtagtgt tgcccttgtg
    17281 cattggtaaa caggtatttt actgaaccat cggttccggg atatcgctca tagccgatgt
    17341 gaccacgcac ctcaccaaat tcatctttta gcgcttttag tatatgcttg ggaatatcat
    17401 ttatatcagc ataattctga attcgcttac gataaatcca tccagtaagc ttaactccat
    17461 agtgggtagg gaatcccaat tccgataacc atgcttcaac ctgctttagg tctttaccaa
    17521 ctagcgtttc ccctttctgc atttaggaat tagtttaaac tgcaaattta gcttttgtta
    17581 taatttttat ttgaataagt gtggtttcag cttcaaacgg ttgaaatata gaatataggc
    17641 aatttattat caaaccatac ttgaaatcaa aattactttg attttaaatt ctttgtaact
    17701 tcgcggagca atgagaaatc ttctaaaaat agtattccct gttgcggcaa tttttttatt
    17761 gctgcttgct tacgggtgca atcggagggt ggcacccaaa agcgctaagg ttgataaaac
    17821 ccgaaagcta aagtgcaggt gtggtaaaac caaatttccc aaaagccatt tccaggttac
    17881 tatgaccagt tttgataaca ggtgatgcta gttaatttaa caatgattgg aatacataaa
    17941 ccatagacaa agatgagtaa actacaggtt aaacttaaca acatttacca gtttgttgat
    18001 gctagcgatg tgctttcgct taagaacaag gtaaacgatt gccaaaagct tattgaaggg
    18061 aagactggaa aagggaacga ttttttgggc tgggtaacct tacctgccga aatcactgac
    18121 gatcagctga aaagcattga aaaggttgcc aatgagctcc gttccaagag tgagtttgtg
    18181 gtagtggtag gtattggcgg ctcttacctt ggagcaaagg ctgtgattga ggccttgggc
    18241 aatagctttt ccatgctcga caacggtggc acaaaggtgc tttttgcagg caacagcatt
    18301 tgtgaggatt accatgccga gcttttaaag ttactcgata agcacaccta ttcaatagta
    18361 gtcatttcaa aatcaggaac taccacagag cctgccatag cattccgcct gttaaaacag
    18421 caccttgagg ggaaggtggg gaaaaccgag gcagcatcgc gtatagttgc aataaccgat
    18481 caatctaagg g
//
LOCUS       DAOOWJ010000049        18299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3054_length_18299_cov_6.392841, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000049 DAOOWJ010000000
VERSION     DAOOWJ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 18299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18299
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3054_length_18299_cov_6.392841"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="PLW22_06395"
     CDS             <1..1354
                     /locus_tag="PLW22_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOU74370.1"
                     /translation="IGSNVGNFFRMNYKTRTLLIGCGAAGAIASIFKAPIAGIVFTLE
                     VLMLDLTTASIVPLLISAVTASSVSYFLMGRNVVFAYDVIRPFALHNIPWFVLLGIFC
                     GLVSLYFIRTIWRIEKRFKQTRSPYKKWIAGTLILGALIFVFPPLFGEGYDVLQALLD
                     GRSEYIFHNSPLYDFQNNYWLLIGSLFLIIALKTVATAVTNGAGGVGGIFAPSLFIGG
                     ISGFLVAKLINQFNFIDVSESNFALVGMAGTMAGVMHAPFMAIFLIAEITGGYALFVP
                     LMITSTIAYLTIMYFEPHSIYTKRLAMRGELITHHKDKAVLTLLNVSKVIETDFLPIE
                     PTETLGSLVKKVSQSRRNVFPVVTTDGKFVGIVMLDDIRPIMFDASRYDTTTVQEIMN
                     LPPEIITINESMESVMNKFEYSKAWNLPVVEGDKYVGFVSKSKIFSVYRNLLIEFSEE
                     "
     gene            1484..2107
                     /locus_tag="PLW22_06400"
     CDS             1484..2107
                     /locus_tag="PLW22_06400"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOU74371.1"
                     /translation="MEIKDVVYSRNVVEFVTVAKEFCAFLEGCEKHTAKSFVGACNKV
                     LPLLYYKATLLPETEPVYDENNEQFVTESDYSSVENKVEYLLGQHNQYVEVNDPRVDE
                     LTGLYTASIAEYMADIYQDLKNFVLRYQVGNTYVMNDALWECINNFKDFWGIRLANLI
                     RAFHILAHQNIDLDNIKHPDESEGSERDTSDWFVTRRQQDMDHNELM"
     gene            2107..2697
                     /locus_tag="PLW22_06405"
     CDS             2107..2697
                     /locus_tag="PLW22_06405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU74372.1"
                     /translation="MFASSIDKETLNSLPRIHFTGQIVVAEKESDLLQWLPMLCQEKI
                     IGFDTESKPSFKKGKINGISLLQLANSKLAILVRLKKTGIPDTLKEFLENPEIVKVGA
                     AIHDDLKGLQKLNRFTPNGFIDLQRIAPTYGIEELSVKKLSAVVLGATVSKGQQLSNW
                     EADVLTEAQKIYASTDAWVCREIYLKLISHTPKQEH"
     gene            2697..3884
                     /locus_tag="PLW22_06410"
     CDS             2697..3884
                     /locus_tag="PLW22_06410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOU74373.1"
                     /translation="MERPRITLKPGKEQSLLRFHPWIFSGAIKSVTEMPNEGQVVDVL
                     GSDGKFLATGHFASGSIAVRVLSFQPVEIDRAFWLSRISNALNLRRAIGLYPNTDTNI
                     FRLVHGEGDLLPGLIVDIYGDTAVMQCHSVGMYLTRTEIANAIMQVLSNTVTAVYDKS
                     SGTLPYREKYNPTDGYISGADNARVPYMEYGNKYHISWTDGQKTGFFIDQRENRLLLQ
                     SYARGKSVLNTFGYTGGFSVSALKGGATEVITVDSSAQAITLANQNIELNFGDNAPHT
                     GVAMDTFEYLRTCNRNFDIIVLDPPAFAKHNDALRNALQAYKRLNLAAIRRLNPGGLL
                     FTFSCSQVVSKTDFRNAVFSAAAISGRQVCIIHQLTQPADHPINIYHPEGEYLKGLVL
                     SVM"
     gene            complement(3889..4662)
                     /gene="yaaA"
                     /locus_tag="PLW22_06415"
     CDS             complement(3889..4662)
                     /gene="yaaA"
                     /locus_tag="PLW22_06415"
                     /inference="COORDINATES: protein motif:HMM:NF002542.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOU74374.1"
                     /translation="MGNYTNYCIVLSPAKTLRSNLKVAGKNYSEPVFLDRAQELVKQL
                     KTYSPVELKDILGVSQDIADLNFTRYANWHLPFTPENSTPAILTFAGDLYEGLQATDF
                     TDDELAFSNRIIFMLSGLYGVLRAFDLMQPYRLEMGSKIQVGQHKSLYDFWGDSITGL
                     LDQNVSDGYLVNLASKEYSRAINDKHFGDRFITIEFKENRPEGLKVIPILSKRARGLM
                     ARFAVKNKIVNAEDLKLFDYEGYGFSDPLSTATRWVFVR"
     gene            4752..7121
                     /locus_tag="PLW22_06420"
     CDS             4752..7121
                     /locus_tag="PLW22_06420"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU74375.1"
                     /translation="MSNNSLPVFKISKNVCHNWWTAIFIVISFIALALPLQSFSRPGT
                     KVVLQLKWKHQFQFAGYYAAIEKGFYAQEDLDVELKELPQNTNVITEVLEGRAQFGIA
                     NADIIIHYVNGAPVVVLAPIFQSSPAALVSRAERNITRPQDIAGKTIEINAKKSGSVE
                     VLTMLSLEGVKPSQYQVTESSLSLNKLLNNQTDASEVYLTNEPYFLEKFGIPYNLILP
                     HKYGVDFYAECLFTTKEMISKNPELVDAFLRASIKGWEYALENSEEIAQVIQSKYKTT
                     KTLDHLIFESQKVKEFIQPNFINVGHSNKGRWLQMMETLLQAGLINKTKPIDALLYNP
                     QQKKHSPFTDKWFILVIGTLLLLTFILHFWYISRVNKRKQQLVNTHKQQVESLKQELQ
                     MLNTHLEETVQRNKDLESFKESLLSNLSFEIRTPLNNIIGYSELLNDPKIKPTQALQF
                     SKEINKSCRLLQNQIDNLIDLSKLESNQFRLVYQRINLMELMNTLQVMLLNELKLFDK
                     EHIAIKAFLDPNEIDFDILSDRNLYKSIFQRLINNAVKFTSKGYIEIGVRKSEKNGHL
                     LFWIQDSGTGIEIEKSKTLFNRLNIEESRDGFGVLQMGLPVVKGIVDLLNGKIWVETA
                     EGTGTTVNVEVPYTPIGSHKAKTYHKKSPFYIHQDPPKLKNKKILIVEDIQSNYILLS
                     RLLEETGCKLEHAKTGDEALKKVENTPDFDLIFMDLRLADMDGIEITRQIRKKNTRVV
                     IVAQTAYSSGVKVNMSIEAGCNDFITKPISRIDLYNVLRKYLVTDDHTA"
     gene            complement(7118..8137)
                     /gene="tsaD"
                     /locus_tag="PLW22_06425"
     CDS             complement(7118..8137)
                     /gene="tsaD"
                     /locus_tag="PLW22_06425"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOU74376.1"
                     /translation="MSVTILGIESSCDDTSAAVIRDQLLLSNVISSQDVHKQYGGVVP
                     ELASRAHQANIIPVVDTALRYAGVTIAEIDAIAYTRGPGLLGSLLVGASFAKGAALTL
                     GVPIISVNHLQGHVLSHFIKDEPNAIHPSFPFLCLLVSGGHTQIMLVRGPMDMEIVGQ
                     TIDDAAGEAFDKCAKVLGLPYPGGPVIDKLAIEGNPGFIKFAKPNIPDLDFSFSGLKT
                     SFLYTLRDKLNTEPNFVNEHLPDICASLQQTIIEILLSKVEQAVRKYRIKEIAIAGGV
                     AANSGLRTALQLLGQKRGWKVYLPKKQFTTDNAAMIAIVGYYSYLAGKTSPLDEPPLA
                     RLPLG"
     gene            8484..12800
                     /locus_tag="PLW22_06430"
     CDS             8484..12800
                     /locus_tag="PLW22_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOU74377.1"
                     /translation="MNNDTLIYAKRVIASISQIDTDAGLFRLGKARVDNAKFYLITDS
                     SGVTNLNALLLKINSDTTQSPDSGRFNLQIGNIVLKNSRFKLVQQGSDSLTPETINFQ
                     NLVLSGINLEASEFNLDGDTIGILINGLTAQDHSGFKIDNLRSKITICSKFMHFNNLR
                     LRANGSSLSMNHLLFDFDGWDSFSNFTSRVRINADFEGTYLHTSTLAYIVTELKNYSV
                     GLRVTGQVKGYVDDLRARNLELGFGNISEIALNANLTGLPKIDNTLFSVDLKKLQTSR
                     KDLMQFKVNGSGKPLIDLPEELDRLGLINYVGRFSGYLSDFVTYGTLTSALGAIDFDV
                     WMKPGKTIKSEFKGKASANNFKLGQLLNENLIGSLSFLSSISGTVNKQGELRAFTDAH
                     IQKLEANGYDYKDIDISGNLGNNSYTGTINLNDPNCKLNFLGRVDFSDTIPVFDFSLF
                     APRIDLVKLNLNRVDSVSVASFLLTAKFSGNTLDNSKGEIKLVNSTYRNQRGEFKIAD
                     LTITADNTNESKVITLKSDIAEGEIRSRQSFSRFPYYMNKVLSRHLPALRQDESNGKS
                     KAPQFNAEEYNDYLIKFRLKKTQKVTSILAPDLSIAENSSLFGILDPNKETLTFKLKV
                     LELVTGSTVFKNLSADGETRDSILEANVLMPEIKVGNSYIRNLKVNATTQNNTVRCNI
                     NWNNYSTPSTLGDINLIADFKAFSQPNSTIDINFLPSSLIINDSTWNLSPSLVRIDSS
                     TILFTNFALSNKHQLISITGKASQLNSDTIAVTLKNIDLSYLNFYLHDSGYKLSGWIN
                     GDALATGILLNPILQAKIGIDKFVVNNQNIGDVIFQSTWHGSENRINLLLSNQRADTL
                     TLLAKGDYFTDKGNLNFDVNINQAKLALIAPLLEGNVSNLQGRMNGSLKIKGDANKPI
                     VDGSIGFDNAGLTIDFLQTHYTLSDKVTINNSNIVLNNFRLTDRFNRTSAINGTIQTN
                     YFKNFNLGLRLNMQNFLCMNTRETDNETFYGTVFASGFVDFLGSPDNLNLNINLKTEN
                     RTAIYLPLSSTSTVEESNFISFVNNNPDLIEIEEAVQNVQTSSSNINITMDLQVTPEA
                     EAQIIIDKKLGDIIKANGSGSLRMEINPSKDVFRMFGRYTIEQGDYLFTLSGVINKRF
                     RIEQGSYIDWNGDPLDANMDIKAVYRVKTSLKQLLLDDSYTARVPVDCKIMLSQKLLT
                     PSIKFGIDFPNLDQQTKALVDGMLNTEEKINTQFLGLLVINSFISDPGMTAAGTQTSN
                     ASLGTTGLYNTASELLSNQLSNWLSQWSKNFDIGINYRPGLENELSSDQVEMALSTQI
                     LDDRVSISSNVGVGGNKNSSNALVGDFTVDIKLNKSGKLRGKAFARNNNDVLLTSQQN
                     NYTTGAGIVYREDFNTIRELFNSIFSKDKQNPNDTLQNDSNTNQNSTNTNLPDTCFVK
                     IN"
     gene            12994..13572
                     /locus_tag="PLW22_06435"
     CDS             12994..13572
                     /locus_tag="PLW22_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU74378.1"
                     /translation="MIPIFLLSVAAVYIFFERYFAIRKASVTDVNFMNRIREYIHEDK
                     IDAAIALCRSQNTPVARMIEKGIQRIGRPLPDVNAAIENVGNLEISKLEESLPFLATI
                     AGGAPMLGFLGTVLGMIRAFYDMSMAGNNIDVGLLSNGIYTAMVTTVAGLIVGIFAYF
                     GYNYLVARIEKVVFNLEANTNEFMDLLYEPVS"
     gene            13585..13986
                     /locus_tag="PLW22_06440"
     CDS             13585..13986
                     /locus_tag="PLW22_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU74379.1"
                     /translation="MALKRRVKVEPSFSMSSMTDIVFLLLIFFMVSSTLIHPNALKLL
                     LPQSNSQVSAKPITSVSITADRNFYLETVPVTLGQLEYLLQQKMKGQDDPTIALHVDR
                     SVPMEEVVKVMNIAKDNKYKLILATQPLQKR"
     gene            13998..14819
                     /locus_tag="PLW22_06445"
     CDS             13998..14819
                     /locus_tag="PLW22_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOU74380.1"
                     /translation="MTNTEYRVRGIVGTILFHALLVFLLIVFGFSTPLPLPAERGILI
                     NFGNSDDGSGIEEPRVQEASSMPQQVQASKPAEESPLTQTHEEAPSLPTPKKESTKKE
                     QTKSKTETTTPQPTQNTQTTQTNTEETPKVNQKALFPGQKTSGSTTGEGETGKPGNQG
                     GLEGSPESTNRTGTTGGGGDAERGIIANLQGRTAQSLPKPEYPSQKEGRVVVEVTVDN
                     RGNVTKAVAGVRGSTTLDNQLLRAAEKAALNAKFNVDPNAPASQIGTITYIFKLQ"
     gene            complement(14828..15577)
                     /locus_tag="PLW22_06450"
     CDS             complement(14828..15577)
                     /locus_tag="PLW22_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOU74381.1"
                     /translation="MDFWLFVLVIFVGAAAGFINTLAGSGSLLTLPVLIFLGLPANVA
                     NGTNRIGILMQSIAAVGGFKKKKVFEWSEGIWLTFPAAIGALIGSMVAVDIDELLMNR
                     IIGVLLVFMFVLLLAKPERWLNPTNPKTNIRLTPLRFVVFMAIGFYGGFIQAGVGYFL
                     LAGLVLGAGVDLVKANAIKVLITFVFTVLALGVFIYNNQVDYLIGITMGVGNMLGAWV
                     ATHVAVKKGAVFIRWFLLVCVFAFALKLLLF"
     gene            complement(15579..16058)
                     /locus_tag="PLW22_06455"
     CDS             complement(15579..16058)
                     /locus_tag="PLW22_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74382.1"
                     /translation="MDTFTIELNEISHKKSKLIRLWIGGALILVVTVALSFYLIGSDI
                     TRLAVLIPVVLQVAIYIYFAYSTYNARVYIQSDAYALEYKFGMLSKTPKTIIWETITK
                     VKIGPTYLAFFKRSGKRTTISLGWLPYVKLKEIKEKICEVAQAKGIACEWAEYKKYS"
     gene            complement(16141..17421)
                     /gene="glyA"
                     /locus_tag="PLW22_06460"
     CDS             complement(16141..17421)
                     /gene="glyA"
                     /locus_tag="PLW22_06460"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOU74383.1"
                     /translation="MKRDTQIFELIEKERQRQMHGIELIASENFVSPQVLEAMGSVLT
                     NKYAEGYPGARYYGGCEVVDQSEQLAIDRLKQLFDAEYANVQPHSGAQANMAVFMAVM
                     KPGDTFLGLDLSHGGHLSHGSPVNFSGMWYKAVSYGVKEDTGRVDYDMMERMAMEHKP
                     KLIVGGASAYSREWDYKRMREIADKVGALLMIDMAHPAGLIAAGLLDNPVKYAHFVTS
                     TTHKTLRGPRGGIILMGKDFPNPWGLTTPKGEVKMMSQILNSSVFPGVQGGPLEHVIA
                     AKAVAFGEALQPEFKEYQTQVKKNAAAMAQAFIDKGYKVVSGGTDNHSMLIDLRTKFP
                     DITGKKVENTLVLADITINKNMVPFDSRSPFQTSGIRVGTPAITTRGLKENHMPVIVD
                     LIDRVISNVDNEKVIAEVRAEVNKLMKDFPLFAW"
     gene            complement(17491..17886)
                     /locus_tag="PLW22_06465"
     CDS             complement(17491..17886)
                     /locus_tag="PLW22_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU74384.1"
                     /translation="MSYPVNNAMGLTGKRILIVDDDTVSRIFLRELLVQSGAVLDFVR
                     NGYELQKYIGSNPEPDVILIDVKLPDADGVELAAQLLAKKPGLRVVAETAYAIEGLDE
                     KCRDYGLKGCVYKPINKDELLKLLLKVAE"
BASE COUNT         5508 a         4203 c         3764 g         4824 t
ORIGIN      
        1 aataggttca aacgttggaa actttttcag gatgaactac aaaacccgaa cactgctaat
       61 tggatgtggt gcagcaggcg ctatagcatc aatttttaag gcacccatag ccggcattgt
      121 gttcaccctt gaagtgctaa tgctcgacct taccaccgca tccattgtgc cgctactaat
      181 ttcggccgtt acggcatcgt cggttagcta tttcctgatg gggcggaatg tggtgtttgc
      241 ctatgatgtt attcggccat ttgcactcca caatattccc tggtttgtgc tgcttggtat
      301 attttgcggg ttggtatcac tttactttat cagaacaatt tggcggattg agaaacgctt
      361 taagcaaacc cggagcccat acaagaaatg gatagcaggg actttaattc ttggcgcgtt
      421 aatctttgtt tttcccccgc tttttggcga aggatatgat gttttacagg ctctgctcga
      481 tggccggtcg gagtatatct ttcacaacag ccccctatat gattttcaga ataactactg
      541 gctgctaata ggctcgctct ttctgatcat tgccctaaaa accgttgcaa ctgccgtaac
      601 caatggtgcc ggaggggttg ggggaatttt tgcaccatca ctttttattg gtggtatttc
      661 aggtttttta gtagcaaagc ttattaacca gttcaacttt attgatgtat cggaaagcaa
      721 ctttgccttg gtaggaatgg caggaaccat ggccggtgta atgcatgccc ccttcatggc
      781 tatcttccta atcgcagaga ttactggggg ctacgctcta tttgtacccc taatgataac
      841 ctcaaccatt gcatacctca ccattatgta ctttgaaccc cactccattt acactaagcg
      901 tctagccatg cgtggcgaac taataaccca ccacaaggat aaagcagtgc tcaccctgct
      961 taacgttagc aaggttattg aaaccgattt tttaccaatt gaacccactg agacacttgg
     1021 ttcgcttgtt aaaaaggtaa gccagtcgcg ccggaacgtt tttcctgtgg taactaccga
     1081 tggcaagttt gtaggcatag taatgctcga cgatataagg ccaatcatgt tcgatgcctc
     1141 acgctacgat accacaaccg ttcaggaaat tatgaacctg ccacccgaga ttatcacaat
     1201 taacgagtcc atggaatcgg ttatgaacaa gtttgagtac tccaaggcgt ggaacttacc
     1261 tgtggttgaa ggcgataagt atgtggggtt tgtatcaaaa tcaaaaatat tctcggttta
     1321 ccgcaacctg ctcattgagt tttcggagga gtagtagggt cagctgttag tgtaaagcgt
     1381 ttggtttttg gtgttaccca aataaatagc aaggggactt taaggaatgt aactaccaac
     1441 ttcaaactat ttccattaac tttgcaccat caatttttat ggcatggaaa ttaaggatgt
     1501 ggtgtatagc cgcaatgtgg ttgagtttgt aacggtggca aaggagttct gcgcttttct
     1561 tgagggttgc gaaaagcaca ccgcaaaatc gtttgttggg gcctgcaaca aggtgcttcc
     1621 cctactctac tacaaggcaa cccttttgcc cgaaaccgaa ccggtttacg acgaaaacaa
     1681 cgaacaattt gttaccgaga gcgattactc ctcggtagag aacaaggtgg agtatttgct
     1741 tgggcaacat aaccagtatg tagaggtaaa cgacccccga gtggacgaac taaccggcct
     1801 atataccgca agtatagccg aatacatggc cgatatctac caggatctaa aaaactttgt
     1861 tttgcggtat caggtaggaa atacctatgt gatgaacgat gccctttggg agtgtatcaa
     1921 taactttaag gacttctggg gtatcaggtt ggctaatctt ataagagcct tccatatact
     1981 ggcgcatcaa aatatcgacc ttgataatat aaagcatccc gatgaaagcg agggttccga
     2041 acgcgatacc tccgactggt ttgttacccg ccgccagcag gatatggatc ataatgagct
     2101 aatgtaatgt ttgcatctag tatcgataag gaaaccctaa attctttacc tcgaatccat
     2161 tttacggggc aaattgttgt tgccgaaaag gagagcgatt tgttacaatg gttacccatg
     2221 ttatgccagg agaaaataat tggcttcgat actgagtcga aaccatcgtt taagaagggt
     2281 aagataaatg gaatctcatt gctgcagctg gcaaacagca agttggctat tcttgtaagg
     2341 ctaaagaaaa caggcatccc tgataccctt aaagaatttt tggagaaccc cgaaatagtt
     2401 aaggttggcg cagccataca cgacgatctg aagggactgc aaaaactaaa tcgctttact
     2461 ccgaacggat ttattgacct tcaacgtata gcgccaacct atggtattga ggagctgagt
     2521 gtaaaaaaac tctcggcggt ggttctggga gctactgttt ccaaggggca acagctcagc
     2581 aactgggaag ccgatgtgct taccgaagca caaaaaatat atgcatcgac cgatgcttgg
     2641 gtatgcaggg aaatctatct taaactcata tcacacaccc cgaaacaaga gcattaatgg
     2701 aacgacccag aataactctc aaacccggta aagagcaatc gcttctgagg tttcacccat
     2761 ggatattttc aggcgcaatt aaatcggtaa ccgagatgcc caacgaagga caggtggttg
     2821 atgtattagg ctccgatggt aagtttttgg ctactggaca ctttgcctca ggatcaattg
     2881 ccgttagggt attgtcgttt caacccgttg aaattgatag agcattttgg ttaagccgaa
     2941 taagcaatgc ccttaacctt cgcagggcca taggcctata tccaaatacc gatacaaata
     3001 ttttccgttt ggttcatggc gaaggcgacc tgctgccagg cctaatagtt gacatttacg
     3061 gcgataccgc cgtaatgcag tgtcattcgg ttggaatgta cctgacacgc accgaaattg
     3121 ccaatgcaat aatgcaagtc ctttcgaaca ccgttaccgc cgtttacgat aaaagttcag
     3181 gtactttacc ataccgagaa aaatacaacc caaccgatgg gtatatcagc ggtgctgata
     3241 acgccagggt accctatatg gaatatggca ataagtatca catatcgtgg actgatggcc
     3301 aaaaaacggg ctttttcatc gaccagcgcg agaataggct actgctgcaa tcgtacgcca
     3361 gaggtaaatc ggtacttaac acatttggat acaccggagg attttcggta tcggctctaa
     3421 agggtggagc cactgaggtt attacggttg atagctcggc gcaagcaatt actctggcca
     3481 accagaatat tgagctaaac tttggcgata atgctccgca caccggcgta gccatggata
     3541 cctttgagta cctaagaacc tgtaaccgaa actttgatat tattgtgctc gacccacctg
     3601 cctttgccaa acataacgat gccctacgaa atgccttaca agcctacaaa aggcttaacc
     3661 ttgctgctat ccgtaggttg aaccctggcg gattactttt cacattctcc tgctcgcagg
     3721 tggttagtaa aaccgatttc cggaatgcgg ttttttcggc agcagccatt tcaggacgcc
     3781 aggtatgcat tattcatcag cttactcaac ctgccgatca tccaataaac atttaccatc
     3841 cggagggtga gtacctgaaa ggactcgtgt taagtgtgat gtaaattatc accttacaaa
     3901 cacccaccta gtagcagtag aaaggggatc gctaaagcca tacccctcgt aatcgaagag
     3961 ttttaaatct tctgcattca ctattttgtt ctttaccgca aagcgcgcca taaggccgcg
     4021 agccctcttt gaaagtattg gaattacctt tagcccctca gggcgattct ccttgaattc
     4081 aatggtaata aaccgatcac caaaatgctt atcgttaata gccctggaat actcctttga
     4141 agcaaggtta accaaatacc catcacttac attctggtca agtaaccccg taatgctatc
     4201 accccaaaaa tcgtaaagcg atttatgttg cccaacctga atcttgcttc ccatttctaa
     4261 acggtatggt tgcatgagat caaacgccct caatactccg tataatcccg agagcataaa
     4321 aatgattcga ttggaaaatg caagttcatc atcagtaaaa tcggtagcct gtaatccttc
     4381 atacaaatcg ccggcaaagg taaggattgc aggtgtgcta ttttccggag taaaaggcaa
     4441 atgccaatta gcataccttg tgaaattgag gtcagcaata tcttgactaa cacctaatat
     4501 atcttttagc tcaactgggc tataagtctt tagctgtttc acaagttcct gagccctatc
     4561 aagaaaaaca ggctcagagt aatttttacc tgccactttt aaattgcttc tcagggtttt
     4621 tgcaggcgaa agcactatgc agtagttcgt gtaatttccc atatctcatc attttttgtt
     4681 ttttgtttca atttcagaaa ttttttgtat ctttaaatta gaaacaaagt agagcatact
     4741 gggttcaaag gatgagtaat aatagcttac cagtttttaa aattagtaaa aatgtttgcc
     4801 acaattggtg gactgccata tttattgtca tttcctttat agctcttgct ttacctttac
     4861 aaagtttttc cagacccggt acaaaggttg tactccagct caagtggaag catcagttcc
     4921 agtttgctgg gtactacgct gctattgaaa aagggtttta cgctcaggaa gatcttgatg
     4981 ttgagctcaa ggagttaccc caaaacacaa atgtgattac cgaggttctt gaaggtaggg
     5041 cccagtttgg gatagccaat gccgacataa taatacatta cgttaatgga gcccctgttg
     5101 ttgtgcttgc acccatattc cagtcaagcc cagcagcact tgtgtcacga gccgagcgta
     5161 atattaccag accacaggat attgctggca aaaccattga aataaatgca aaaaaatcgg
     5221 gttcggttga agtgcttacc atgcttagcc ttgagggtgt taaaccctcg cagtaccaag
     5281 taacggaatc gtcgctttca cttaacaaac tgctgaataa ccaaaccgat gccagtgagg
     5341 tttacctgac aaatgaaccc tatttcctcg aaaaatttgg gataccctat aacctgattc
     5401 tgccccataa gtatggcgtt gacttttacg ccgaatgctt atttaccacc aaggagatga
     5461 ttagtaaaaa tcctgaactg gttgatgctt tccttagagc atccattaaa ggatgggagt
     5521 acgcccttga aaattcggag gaaatagcgc aggtaattca atcaaagtat aaaaccacaa
     5581 aaactcttga tcacctcata ttcgaatcgc aaaaggtgaa agagtttatt caacctaact
     5641 ttataaatgt tgggcattca aacaaaggcc gatggctaca aatgatggaa acccttttac
     5701 aggccggatt aattaacaaa acaaaaccta ttgatgcttt gctttacaac ccacagcaaa
     5761 aaaaacattc accttttacc gataaatggt ttattttggt tattggcaca ttattgctgc
     5821 taactttcat attacacttt tggtatatta gtagggtaaa taagcgcaag caacaattag
     5881 taaacaccca caagcaacag gttgagagct taaagcaaga gttacagatg ttaaacaccc
     5941 atctggaaga gactgttcaa cgaaacaaag accttgaatc gttcaaggaa tcgctactct
     6001 ctaacctctc gtttgagatt cgaaccccac tcaataacat aattggttac agtgaacttt
     6061 taaacgaccc aaaaattaaa cccacgcaag cgcttcaatt ttccaaggag attaataaaa
     6121 gttgccgtct actccagaat caaattgata atcttataga cctctcaaag ctggaatcga
     6181 accagttccg gttggtttac caacgcatta acctgatgga acttatgaac accctgcagg
     6241 ttatgctgct gaatgagctt aagctctttg ataaagagca tatcgccata aaggcattcc
     6301 ttgaccccaa tgagattgac tttgatatcc tatcggaccg aaacctttac aagagtatat
     6361 tccaaaggct aattaacaat gctgtaaagt ttacgtcgaa aggttacatt gagattggcg
     6421 taagaaaatc ggaaaaaaat ggtcacctcc ttttctggat acaggatagc ggaacaggaa
     6481 tagagattga aaaatcaaaa acattattta accgtttaaa tattgaagag tcgcgcgatg
     6541 ggtttggtgt actacaaatg ggactccctg ttgttaaggg tattgtggat ctgctgaacg
     6601 gaaaaatttg ggttgaaaca gctgagggta ctggaactac tgtgaatgtt gaagttccct
     6661 acacgccaat tggcagccac aaagcaaaaa catatcataa aaaatcacca ttctacatac
     6721 atcaagaccc tccaaagctg aaaaataaaa agattctgat tgttgaagat attcaatcga
     6781 actacattct gctaagcaga ctcctggaag aaactggttg taagctagag catgccaaaa
     6841 ccggagacga ggcactcaaa aaggttgaaa acacccccga ctttgaccta attttcatgg
     6901 atttaaggct agctgacatg gatggtattg agattacgcg ccaaatccga aagaagaaca
     6961 ccagggtggt tattgtggca caaaccgctt acagttcagg agtaaaagta aacatgagca
     7021 ttgaggcagg ctgtaacgat tttattacca aaccaataag ccgcatcgac ctttacaatg
     7081 ttcttcgtaa gtaccttgta accgacgatc acacagccta acccaggggt aaccgagcta
     7141 aaggaggctc atcaagcggc gatgtctttc cagcaaggta gctgtagtaa cccacaatgg
     7201 caatcattgc agcattatcg gtagtaaact gcttcttagg taggtaaacc ttccatcccc
     7261 tcttttggcc aagtagctgt aaagcggttc gtaatcctga atttgctgcc acgcctccag
     7321 caatggcaat ttcctttatc ctgtacttac gtacagcctg ctcaacctta cttagcagta
     7381 tctcaataat ggtttgctga agcgaggcgc aaatgtcggg taggtgttcg ttaacaaagt
     7441 ttggttcagt gtttagttta tcgcgcaggg tataaaggaa tgatgttttt aatccgctaa
     7501 agctaaaatc gaggtcgggt atattcggct tggcaaactt tatgaatcca gggttaccct
     7561 cgatagcaag tttgtcaatt acaggcccac cagggtaagg cagcccaagc accttggcac
     7621 acttgtcgaa tgcctcgccc gctgcatcat caattgtttg gccaactatt tccatatcca
     7681 ttggaccccg aaccaacatg atttgggtgt ggccccctga gaccaaaagg cataaaaagg
     7741 ggaacgatgg gtgaatagca ttgggctcat ccttaatgaa gtgcgaaagc acatgaccct
     7801 gtaagtgatt aaccgatatt attggaactc ccagtgtgag cgccgctccc tttgcaaacg
     7861 aagcccctac aagcaacgag cccagcaacc ccggacctcg cgtgtaggct attgcatcta
     7921 tctccgcaat ggtaacccca gcgtaacgca gggcagtatc tactacagga atgatgttag
     7981 cctgatgagc gcgcgaggct aactcaggca caactccacc atactgttta tgaacatcct
     8041 ggctgctaat tacattcgat aaaagtagct gatcgcggat aactgctgct gaggtatcgt
     8101 cgcacgatga ttctatccca agtattgtta cgctcatcaa taaaaattga aataatacaa
     8161 aaaaagttta aaaacctatc ctcacctatt tcaaaagccc aagcatttgt atattttagg
     8221 aacaaatttt gatgcccaca ttccagaaat aacttagcaa aggtattaaa aaactactta
     8281 aaatactgat ttgggggttt gttacactac tcgcaatccc tacgcttgct tggctgctgc
     8341 tgcaaagtag ccaggtacaa acctatatta ttaataaaac tacccattat ttacaccaaa
     8401 aaacaggaat tactgtaaac attagaaaag ttgatgttag gccatttaca ggcattttac
     8461 taaaagatgt ttacgttgcc gatttgaaca acgacaccct aatctacgca aaaagagtaa
     8521 tagcatccat ttcacagatt gataccgacg ccgggctttt ccggttagga aaagcaaggg
     8581 ttgacaatgc aaagttctac cttattactg atagttcagg ggtaacaaac ctaaatgctc
     8641 tcctgcttaa aattaattcc gacaccactc aatcgcccga ttcgggcagg tttaatctgc
     8701 aaattggaaa tattgttctt aagaactccc gttttaagct tgttcagcaa gggtccgaca
     8761 gccttacgcc ggaaacaata aactttcaga atcttgttct ttcaggaatc aatttggaag
     8821 caagcgagtt taacctggac ggcgatacca ttggaatact aataaatggt ttaacggcac
     8881 aggaccattc cggatttaaa atagataacc tgcgaagtaa gattaccatt tgcagtaagt
     8941 ttatgcattt caataacctg cgcctgcggg caaatggctc gagcctatcc atgaaccatc
     9001 ttttattcga cttcgacggg tgggatagct tcagcaattt tacttcacgg gttagaataa
     9061 atgccgactt tgaaggcacc tacctgcata ccagtacctt ggcctacatt gttaccgaac
     9121 ttaaaaatta tagcgttggg ttaagggtaa ccgggcaagt taaaggctat gtggacgatt
     9181 taagggcacg gaaccttgaa ctgggctttg gcaatatttc agagatagcc cttaacgcca
     9241 acctgaccgg cttacccaaa attgataata cccttttctc agtggacctg aaaaaacttc
     9301 agacttctag aaaagatctg atgcaattca aggtaaatgg tagtggaaaa cccctaatcg
     9361 atttgcccga agagcttgat cgccttgggc taataaacta tgttggaaga ttctccggct
     9421 acctcagcga ctttgttacc tacggtaccc taacatcggc tctaggggca attgattttg
     9481 atgtttggat gaaacctggc aaaaccatta aaagtgaatt taagggcaag gcatcggcta
     9541 acaatttcaa gcttggccag ctcttaaacg aaaatttaat cggaagcctg tcgttcctat
     9601 ccagcatatc cggtacagtc aataaacaag gagaacttag agcttttacc gatgcccata
     9661 ttcagaaact cgaggctaat ggatatgatt ataaagatat cgatatatcc ggtaatctag
     9721 ggaacaactc atacaccgga accatcaacc ttaacgaccc caactgtaag cttaactttt
     9781 tgggtagggt tgacttttcc gacaccattc ccgtgttcga tttctcactc tttgcccctc
     9841 gcatcgattt ggttaagctc aaccttaaca gagttgactc cgtttcagtg gcatcgttcc
     9901 ttttaaccgc aaagttttct ggcaataccc tcgacaatag taagggcgaa ataaaactgg
     9961 ttaactctac ctaccgtaac caacgcggcg agtttaagat tgccgacctt acaattactg
    10021 ccgataacac caatgaaagc aaggttatta cgcttaaatc cgatattgcc gagggtgaaa
    10081 tacgtagtag gcaaagcttt tctaggttcc cctactacat gaacaaggtt ctgtcgcgcc
    10141 acttacctgc ccttaggcag gatgaatcca atgggaaatc caaagctcca caatttaacg
    10201 ctgaggagta taacgattac ctcatcaagt ttaggttaaa aaagacacaa aaggttacaa
    10261 gcattttagc gccagaccta agcattgccg aaaactcatc gctttttggc attttagatc
    10321 ccaacaagga aaccctaacc tttaagctta aggtacttga actagtaaca ggcagcaccg
    10381 tttttaaaaa tctaagtgct gatggcgaaa cccgcgatag cattctggaa gccaatgtgc
    10441 taatgcccga aataaaggtt ggcaacagct atatccgaaa ccttaaagta aacgctacta
    10501 cccaaaacaa caccgtaagg tgtaatatta actggaataa ctactcaaca ccttctaccc
    10561 tgggcgatat taacctgatt gccgacttta aagcgttctc gcaacctaac agcacaatag
    10621 atattaattt tctaccctca tcgcttatta taaacgactc cacatggaac ttgtctccat
    10681 cgctggttcg tatcgattca tctaccatac tgtttaccaa ttttgccctc tccaataagc
    10741 accaactaat tagcataacc ggtaaagcgt cgcagcttaa ttcggacact atagcagtaa
    10801 ctctcaaaaa catcgacctg tcttacctga acttttacct gcacgattcc ggttataagc
    10861 tgagcggttg gataaatggc gatgccttgg caactggcat cctgctaaac cctatactac
    10921 aagccaagat tgggattgat aagtttgtag taaataatca gaatattgga gatgttatat
    10981 tccagagcac ctggcacggt agcgagaata ggattaacct tttactaagc aaccaaaggg
    11041 ccgataccct aacactcctt gcaaaaggcg attactttac cgataaaggc aacctcaact
    11101 ttgatgtaaa cattaaccag gcaaagttag ccctaattgc tcccttactg gaagggaatg
    11161 tttccaacct acagggccgc atgaatggct cacttaaaat taaaggcgat gccaataaac
    11221 ccatagtgga tggtagtatt ggctttgaca atgccggact aaccattgat tttctacaaa
    11281 cccattatac cctatccgac aaggtaacca taaacaacag caatattgta ttaaacaatt
    11341 tcaggttaac cgataggttt aatcgaacat cggctataaa cggaaccatt caaaccaact
    11401 actttaagaa tttcaacctt ggacttaggt tgaacatgca aaatttcctc tgcatgaaca
    11461 cccgtgaaac cgataacgaa acgttttatg gaactgtttt tgcctccggg tttgtggatt
    11521 ttttgggttc gcccgacaac ctaaacctca atataaacct taaaaccgag aacagaacag
    11581 caatttattt acccctttcc tccacaagca ctgtggaaga atcaaacttt atcagctttg
    11641 taaacaacaa ccccgatttg attgagattg aggaggcagt gcagaatgtt caaacctcat
    11701 catccaacat caacattacc atggatttac aggtaacccc cgaagccgag gcgcaaatta
    11761 ttatcgacaa gaaactaggc gatattatta aagcaaatgg cagcggtagc ctgcgaatgg
    11821 aaattaaccc gtcaaaagac gttttccgta tgtttggccg ctacaccatt gagcagggtg
    11881 attacctttt caccctctct ggggttatca ataagcgttt ccgcattgag cagggcagtt
    11941 acatcgattg gaatggcgac ccgctagatg ctaacatgga tatcaaggcc gtgtaccggg
    12001 taaaaacctc acttaagcag ctcttgctcg acgatagcta tacagcccgt gttccggttg
    12061 attgtaaaat catgctatcg caaaagctcc ttacaccatc aatcaagttt ggtatcgact
    12121 tccctaacct cgatcagcaa accaaggctt tggtcgacgg aatgctcaat accgaggaaa
    12181 aaataaatac ccagttccta gggctgctgg taataaacag cttcatttcc gaccccggaa
    12241 tgaccgcggc tgggacacaa acctccaatg ccagccttgg cactaccggc ttatacaata
    12301 ctgccagtga gttactctcc aatcagctaa gcaattggct ctcccaatgg agcaaaaact
    12361 ttgatatagg tataaactac cgcccgggtc tggagaacga gctcagctct gaccaggttg
    12421 aaatggcgct atcaacccaa atactggacg atagggtttc cattagtagt aacgtgggtg
    12481 tgggtggcaa taagaactct agcaatgcac ttgttggcga ctttaccgtt gatattaagc
    12541 tcaacaaaag tggtaaactc cgtggtaagg cctttgcacg taacaataat gatgttctgc
    12601 taacaagtca gcagaacaac tacaccactg gtgcaggtat tgtttaccgt gaggatttta
    12661 acaccatcag ggagcttttc aactcaatct tcagcaagga taaacagaat ccaaacgata
    12721 ctttacaaaa cgatagcaac accaaccaaa attctaccaa tacaaacttg cccgatactt
    12781 gctttgttaa gataaattag cactgtgctc acctatttga acaatgaaag gtggaataat
    12841 acgttttttt actaattttc ggaaaaattt aaaataatga atacgatgct atccatgttt
    12901 gttcttcttc aaactccggc cggtgttaat cccggaacgc aaggtgaaat atctttaagt
    12961 tttctggatc ttgcccttaa aggcggatgg ataatgattc cgatattttt actatcggta
    13021 gcagccgttt acatcttttt tgagcgctac tttgccattc ggaaagcctc agttaccgat
    13081 gtcaatttca tgaaccgtat cagggagtat atacatgagg ataaaataga tgctgctatt
    13141 gccctttgcc ggtcgcaaaa cacaccggta gcccgcatga tagagaaggg aatacagcgc
    13201 attggtcgcc cgctgcccga tgtaaacgca gccattgaga atgttggtaa cctggaaatt
    13261 tcaaagctcg aggagagctt gccattcctg gcaaccattg ctggcggtgc ccccatgtta
    13321 ggattcctag gaactgttct aggtatgatc cgtgcctttt acgatatgtc catggctgga
    13381 aataacatcg atgtgggctt gctctcaaac ggtatctaca ccgccatggt gacaaccgta
    13441 gccggtttaa ttgttggtat tttcgcatac tttggataca actacctggt tgcccgtatc
    13501 gaaaaggttg ttttcaacct tgaggccaac actaacgagt ttatggattt actctacgaa
    13561 cctgtttcgt aaaaatttac ccctatggca ctcaaacgaa gagtaaaggt tgaaccgagc
    13621 tttagcatgt catccatgac cgatatagta ttcctattac ttatcttttt catggtatcg
    13681 tcaaccctta ttcaccccaa tgcgctaaag cttttactac cccaaagtaa cagccaggta
    13741 tcggcaaagc ccataacctc ggtttccata actgccgata ggaattttta cctggaaacg
    13801 gttcccgtta ccctcgggca actggaatac cttttgcagc aaaagatgaa aggacaggac
    13861 gatcctacca ttgcactaca cgttgataga agcgtaccca tggaggaggt ggttaaggtt
    13921 atgaatattg ccaaggataa caaatacaag ctaattctgg caacccaacc attacagaaa
    13981 aggtagtaag aataaaaatg acaaacaccg aatatagagt aagaggaata gtgggaacaa
    14041 tactgttcca tgcacttctt gtattcctat tgatagtatt cggttttagt accccgttgc
    14101 cattgccggc cgagaggggc atcctaataa actttggtaa ttccgacgat ggtagtggaa
    14161 ttgaagaacc tcgtgttcag gaagcatcgt caatgccaca acaggtacag gcaagcaaac
    14221 ccgctgagga atcgccatta acccaaaccc acgaggaggc gccatcgctt cctaccccaa
    14281 agaaggaatc gaccaaaaag gagcaaacta aaagtaaaac cgaaaccact actccccaac
    14341 ccacccagaa cacccaaacc acccaaacaa atactgaaga aacgccaaag gtaaaccaaa
    14401 aggctctttt ccctggtcag aaaacctccg gtagcactac cggtgagggt gaaaccggta
    14461 aacccggcaa ccagggtgga cttgaaggat cacctgaatc gaccaaccgt actggaacta
    14521 caggcggtgg gggcgatgct gaaaggggca ttattgcaaa ccttcagggc agaaccgccc
    14581 aaagccttcc aaaaccggaa tatccatcgc aaaaggaagg acgtgttgtg gttgaggtta
    14641 ctgtggataa ccgaggaaac gtaacaaagg ctgttgcagg ggttaggggt tcaactacgc
    14701 tcgataatca gctactacgt gccgctgaaa aggcagccct taatgccaag ttcaatgttg
    14761 atcccaatgc tcctgcttcg caaattggga caataactta catttttaaa cttcagtaat
    14821 tcaagggcta gaatagaagc aattttaagg caaaagcgaa cacgcacacc agcaaaaacc
    14881 aacgaataaa cacggcaccc ttcttaacag ccacatgggt tgcaacccag gcccctaaca
    14941 tatttcctac ccccatggta atgccaatca ggtaatctac ctgattattg tatataaaca
    15001 ctcctaaagc caacacggta aatacgaaag taatcaacac ctttatggcg ttagccttaa
    15061 ccaaatccac gcctgcacca agaactaacc cggccagtaa aaagtaacct acaccggcct
    15121 gaataaaacc accatagaag ccaatagcca taaatactac aaaccgtaag ggagttaaac
    15181 ggatgttagt tttggggttt gttgggttga gccatcgttc aggcttagcg agtaataaaa
    15241 caaacataaa caccaaaagc acaccaatta tacggttcat taggagttca tcgatatcaa
    15301 ccgccaccat tgaaccaatt aaagcgccaa tagctgcagg gaaggtaagc caaataccct
    15361 cgctccattc aaaaaccttc tttttcttaa acccgccaac agcagcaatg ctctgcatta
    15421 aaatcccaat tcggttagtg ccattggcta cattggctgg caagccaaga aaaatgagta
    15481 cgggtagagt aagcaacgac ccacttcctg ccaaggtatt gataaatcct gctgctgccc
    15541 caacaaaaat tacaaggaca aaaagccaaa aatccatctt agctatactt tttgtattca
    15601 gcccactcac aggctatgcc ctttgcctgg gcaacctcgc aaatcttctc ttttatctcc
    15661 ttcagcttaa cgtatgggag ccagcccaag ctaatggttg tgcgcttgcc cgaacgcttg
    15721 aaaaaagcta ggtaggtagg gccaatctta actttagtaa tggtttccca gattatcgtt
    15781 tttggggttt tgcttagcat gccaaatttg tactcaagag cataggcatc ggactggata
    15841 tatacacgtg cattgtaggt agagtaggca aagtagatgt aaatggcaac ctgtaatact
    15901 accggaataa gaacagctaa acgggtaata tcgcttccta ttaagtagaa cgaaagcgca
    15961 actgtaacta ccaggattaa agcaccacca atccaaagcc tgatcagctt tgatttttta
    16021 tggctaattt cgttaagttc aatggtaaag gtatccatag ccagctaatt tttcatgcaa
    16081 tttactaaaa aaccccaata gcctatgcca ttggggtcaa attatttagg ttgataccga
    16141 ttaccaggca aagagtggga aatccttcat tagcttgttt acctcagccc taacctcggc
    16201 aataactttc tcgttatcaa cattggatat caccctgtca atcagatcca caataactgg
    16261 catgtggttt tcctttaaac cacgggtggt aatggcagga gtaccaacac ggatacccga
    16321 ggtttggaat ggggaacggc tatcgaaagg aaccatgttt ttatttatgg tgatatcggc
    16381 tagcaccagc gtgttttcca cctttttacc ggtaatatcg gggaatttag tacgcaggtc
    16441 gataagcata gagtggttat cggttccacc cgaaaccacc ttgtagcctt tatcaataaa
    16501 ggcttgcgcc atagcggcag cattcttttt cacctgggtt tgatactcct tgaactcagg
    16561 ttgaagggct tcgccaaaag caactgcttt tgctgcaata acatgctcaa gagggccacc
    16621 ctgaacaccg gggaaaacac tggagttcag tatctgcgac atcattttca cctcaccctt
    16681 aggagtggtt aaaccccatg gattggggaa atccttaccc atcagtatga taccaccgcg
    16741 ggggccgcgg agggttttgt gagtggtaga tgtaacaaag tgagcgtatt tcacaggatt
    16801 atcgagcaaa ccggcagcaa taagacctgc agggtgggcc atgtcaatca ttagcaatgc
    16861 gccaacctta tcggcaatct cgcgcatacg cttgtagtcc cactcacggg agtaagccga
    16921 ggctccacca actataagct ttggcttatg ctccatggcc atgcgttcca tcatgtcgta
    16981 gtcaacccta ccggtatctt cctttacacc gtacgatacc gccttgtacc acatccctga
    17041 aaagttaacg ggcgaaccgt gagagaggtg accgccatgc gaaaggtcga gtcccaggaa
    17101 ggtgtcgccg ggtttcatca cggccatgaa aacggccatg tttgcctgcg cccccgagtg
    17161 aggctgaacg ttggcatact cggcatcaaa tagctgtttt agcctgtcaa tggcaagctg
    17221 ttccgactgg tcaaccacct cgcaaccacc gtagtagcgt gcaccgggat acccctcggc
    17281 atacttattg gttaacaccg accccatagc ctcaagaacc tgagggctaa caaagttttc
    17341 cgatgcaata agctcaatgc catgcatctg gcgctggcgc tccttttcaa tcagttcaaa
    17401 aatctgagta tcgcgtttca ttctattagt atttaaaatt atgccgtaaa attaaccttt
    17461 taaaaataat tcaaatacga gcgccatgtg ttactcagca acttttagta aaagttttag
    17521 aagctcatcc ttgttgattg gcttgtacac gcaccccttt aaaccgtaat cacgacactt
    17581 ttcatcgagc ccttcaattg cataggcggt ttcggcaaca acccttaagc ctggcttttt
    17641 agccaatagc tgagctgcaa gctcaactcc atcggcatcg ggtagcttaa catcaataag
    17701 tataacatcg ggctccgggt ttgagccaat atatttttgc aactcataac cattacggac
    17761 aaaatccaac actgcacccg actgcactag caattcccta aggaatatcc ggctaaccgt
    17821 atcgtcatca acaatcaaaa tcctctttcc ggttaggccc atggcattat ttactggata
    17881 ggacatggct tttggttttt agtcaactta ttttaccaaa tataatttta atttactgaa
    17941 tttcaaaata gtgacaattt gttactcaaa gttaaataat acaaatccct ttcgtttagt
    18001 taacaaaaat atacatgcct gtcatcctaa gcggcctgtc ccaccctagc ggggagcgaa
    18061 tgatctctaa tcgaccccat gagacgtttc gctcgcgttc aacatgacaa taacttcacg
    18121 aacaacgatt cacgaacaac gaataacgtt ctcagcccca taggggcgaa acatttgtaa
    18181 ctccatgaaa cttccgtatg tacagcgatg cacgcaatac ataatccaaa gagcaaaggt
    18241 gaagcgttgc atcgcaacag aaaagcatgg caaagagcgg tttacatgtt ctttcttgt
//
LOCUS       DAOOWJ010000050        18269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3061_length_18269_cov_6.933019, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000050 DAOOWJ010000000
VERSION     DAOOWJ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 18269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18269
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3061_length_18269_cov_6.933019"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PLW22_06470"
     CDS             complement(<1..1081)
                     /locus_tag="PLW22_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74385.1"
                     /translation="MSSNDFIKDRLRVKLLAFPAAELSVFDKAKKKYMGLFNPEEMEF
                     VNDKPDVLMFLTGGSEHIAIESVQEYRFYLLLASREANAWAAATEVKAWMNQHNIRSV
                     LLDADNPSTAEYLSEYYNAFVGVRKLHGQRLGVVGSSSPWLVASGVNPYLLQSKLGVE
                     QVDISWDDIVFDEISQVSPDFTSLFGMAENRVELIESGKIYEGLASLIPFYRLNAMAV
                     ECFPLVNKTGHTACLALAKLNYDGIPTACEADICSAAAMMLSSEVCNAIPWMANVAHV
                     GNGKAILTHCTAPVNHLTSFKLDTHFETGKGYAVSGQLKGDEVTIFRFDSTLNHIFVT
                     LARVTDKPKSRNACRTQLEVELSPSA"
     gene            complement(1074..2240)
                     /locus_tag="PLW22_06475"
     CDS             complement(1074..2240)
                     /locus_tag="PLW22_06475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOU74386.1"
                     /translation="MNWKRKLKVASVVVVVALAVYLALPQNHYIVRALIYQQVNIDDY
                     KIFHNRTVKAGTPQPWRLHEKYNSYQLTNDQLKYFNDFRTVAFLVVKDTALLFESYWD
                     GYGKDSYSNSFSMAKSIVSLLMGCALSDGYVKSIDEPVGNYLPEFAEGDKAKITIKHL
                     LTMSSGLSWDESYSTLFSLTTQGYYGKNLPKLVLNQTVVKEPGKTFEYRSGDTQLLSL
                     IIEKATGKTLADYASEKIWSKIGAEHDALWCLDRKDGVEKAFCCFNTNARDFARFGQL
                     VLNGGRWNGEQIVPEEYLNESLTPASYLFDPETNKNVDFYGYQWWMINVDGYKTWYAR
                     GLLGQYIFVIPSLNAVVVRLGHVRNNNRIDGTPEDVYQYIRFGISIVKSIENNE"
     gene            2339..3406
                     /locus_tag="PLW22_06480"
     CDS             2339..3406
                     /locus_tag="PLW22_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74387.1"
                     /translation="MKSIMLPIALISTFIAIVYKSNAQPDSSGVGLYLLGQSFFDNKE
                     FTGNIKKGYTHPGFYIQPAIKFKTEQYSIAAGFHTLYLAGADSLERLVPVFSASLQLN
                     SRVTLIVGTLESKNGHWLPEPLFKPERLFLNQPETGVQFLYRGTESKADLWINWERYI
                     KTGSPFQEEFTVGFTHLYHRNKNTGFSNAFHAMAFHHGGQIDSTNLPVQTHINLGVIP
                     AYSFQPLAKTRFEFSSSLYYYKNLSPSFTSYYSSGFGLHPQLRAYHNSTTIDIGYWYS
                     DGFINPRGEELFGSISTINPTFNQAVRTMGTFSIEFQKVFRESLLLNANFKAYYDFKS
                     GLLEYSYTLRLMFDGKLTRVK"
     gene            complement(3580..3843)
                     /locus_tag="PLW22_06485"
     CDS             complement(3580..3843)
                     /locus_tag="PLW22_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOU74388.1"
                     /translation="MTRITFSKNSWEEYISWQNEDKKMLKKINELIKDIQKHPYEGIG
                     KPEPLKYDLAGLWSRRIDREHRLVYQVIDQEILIYSCRYHYDR"
     gene            complement(3840..4094)
                     /locus_tag="PLW22_06490"
     CDS             complement(3840..4094)
                     /locus_tag="PLW22_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOU74389.1"
                     /translation="MMAANFTEFRTNLKKFLDNVVNNNETLIIKRGTGKGAVMMSLDE
                     YNSIMETLHLLNSKANADRLYESIKQMKEGNVVRNKLIEE"
     gene            complement(4164..5408)
                     /locus_tag="PLW22_06495"
     CDS             complement(4164..5408)
                     /locus_tag="PLW22_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74390.1"
                     /translation="MKRTVVILVGLVIIAFAAYILLKPRLAQNSYNEGLKLLDSAKYE
                     LALEKFNRAIKLNPKRYDYFFKRGVTLAALNNDSLALADFSTAIGIDSTHFELYIERG
                     VSYRKLKQYSQAMTDLNRAIAMNDSSSKAYYQRGLLNAATMDFEQAIKDYNRSIELDG
                     SNLEAFYSRGESLANLADFKGAIADYDKMIEGANATPEAYKKRGIFKLKIQDYNGAIA
                     DLEVALKELPKDEEALYSLGDAFANVQRFDDAIKVLDKAIAVNPKLPLAYGVKGGALL
                     RKGEFKQAVVAFNKAIELKPDYLKAYYGRGVARAFQKDYKGSITDFNKVISIDPNYVQ
                     AYYNRAVSKAIMGNHKDAIPDYDVIIKADPKNAEAYYNRGISKMNVQEFKSGCDDFRK
                     AVELGYKPAEQMVNIYCPKENK"
     gene            complement(5462..5800)
                     /locus_tag="PLW22_06500"
     CDS             complement(5462..5800)
                     /locus_tag="PLW22_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74391.1"
                     /translation="MLNNDMVSFCPVADSTGWYLNVIDYPNSVDFRKHAQKICSTYTL
                     LKTVNDNTYRNYSDSYSFYLRSYKSLFFISQHDDPNYHKLTDLLSNQNPRFAREVAKL
                     NCALIVEANK"
     gene            complement(5806..6438)
                     /locus_tag="PLW22_06505"
     CDS             complement(5806..6438)
                     /locus_tag="PLW22_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU74392.1"
                     /translation="MTSTYRYVFKGLLALLLFGIVVSCSKDDEIVDPTSKPDPEIAQL
                     TKKIHADSIAATIGMLQGMGTRFALAENRRDVAVAIMNKFKSYGYATARLDSFQLNVY
                     FRNQYYETWQYNVVCTLPGVEFPDSLTIIGGHHDCILRSTEPESPLDVAPGADDNASG
                     VAVTLEIARVLKQSGFAPRTTIVFATFAAEELGLYGSQHMTNRLSDTLAR"
     gene            6561..9629
                     /locus_tag="PLW22_06510"
     CDS             6561..9629
                     /locus_tag="PLW22_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU74393.1"
                     /translation="MENLSVSQFKKYNNLIGWFTFAIATLVYLLTIEPTASFWDCSEF
                     IASGNKLEVGHPPGAPFFMLMVRFFTMFAPSPQWVPILANSLSALASSFTILFLFWTI
                     TYMARRIVSPKATEFTLTQVILILSSGFVGALAYTFSDTFWFSAVEGEVYATSSLFTA
                     LVFWAILKWEEQADQPHATRWIILIAFLMGISIGIHLLNLLAIPAIALVYYFRKYSYS
                     PKGLFYTLLISGVLLITIMYGIIQGFVVIASKVELLFVNSFGLPIKSGVFFLFTVVLA
                     LLAYGIYRTHKQGRVVLNTIFVGLTVIMIGYSSYAAIVIRSSANPPMDQNSPDNMFSL
                     LYYLNREQYGDRPLLYGQVFNAQPIARVEGEPTYAPKNGKYVFVRTKDKYEFDPEFKM
                     LFPRMWSNEQNHVNAYKKWSNFDGNPIKRFGPDGKTETIYVPTFGENLLFFFRYQLGF
                     MYFRYFMWNFAGRQNDLQGHGEVTKGNWISGIPFIDNPRLGPQEKLPEIYRNNKGRNQ
                     YYLLPLLLGLAGLLYHLSRHKHDFWVTTMLFVMTGIAIVVYLNQTPYQPRERDYAYAG
                     SFYAFTIWIGLGVLAVYELLKKIKDTPATAVVTASVCTLAVPVLMASENWDDHDRSGS
                     YVPVDFGYNMLIGCKPNAILFTYGDNDTFPLWYSQEVEGVRTDVRVANLSYLRGEWYI
                     EQMQRKAYQSDPIPIRMTYDQYFTGARDAIVVIDRIKEPLNLDRVVDFFLSDKPENMI
                     QSPFNQNERINYIPSKQLFLTVDKSEVKSKVDLPDNYLNRIADTMTWTLNKQVVLKDG
                     QVILDLIATNQWRRPIYYATTVSGETYLGLDKYFHLEGMMYRVLPIPASRQWGTGSVN
                     TREMWENLMEKYRFRSLNNPKVYLDENKMRIVSNYRNLFARLANALVDEGKMDSAITA
                     LNRCMELIPPKNVPLNYFALSMVEGYYRAGDMQNALKYSQQMVNQASEAISYMIYDLD
                     EFQKNWLGNELQLNLAILYELTRMANQYEKGEYAKQLTDKFNSYVTVLEG"
     gene            9632..10249
                     /locus_tag="PLW22_06515"
     CDS             9632..10249
                     /locus_tag="PLW22_06515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU74394.1"
                     /translation="MINLSPPRFFQRFFRNVIWNFPSEKEGVFLTFDDGPTPGVTPWV
                     LEQLRKYNAKATFFCLAKNIEMHPEIFEMIVADRHAVGNHSYSHIKGWGTDTTQYVQD
                     IIYANSFVNSNLYRPPYGRITPKQFKLLKSQYKIILWDVLSMDYSRRVSPKRVYQYVV
                     RNAKPGSIIVFHDSLKAERNLRYALPRVLDNLSSKGLTFKSIIIP"
     gene            10263..11351
                     /locus_tag="PLW22_06520"
     CDS             10263..11351
                     /locus_tag="PLW22_06520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU74395.1"
                     /translation="MSNILFIVQLPPPVHGASLMNKLVMGRASQLPNNKCFTISLNFS
                     KSLSDLQRFKPRKILRAITVVFEIIGKIIKYKPDIVYFSMVPIGLVLIRDSFYLMLCK
                     ALSPKSKKIIHLHRPGMLQFYSQKPYLYRFYSFIFKNCEIVHLTPRLARLELSPLNLK
                     KTKITVIPNSIIKTPEYERHEKDWSNILFLSNFLIHKGHFELVEAFAILSSKYPQLKL
                     TLAGAFPSETEEQNLLERIKQYGLNRTVEVVGPVYGPKRFELYSRAGIFVLPSKLEYF
                     PLVILEAMSERCAVITSGKENLQDTFVDGKQLLFLESVDPYDISQKIERLIQSPQLTQ
                     SIANEGYKRFTEIQEESLLKIDKLFTCQ"
     gene            11445..12534
                     /gene="prfB"
                     /locus_tag="PLW22_06525"
     CDS             join(11445..11507,11509..12534)
                     /gene="prfB"
                     /locus_tag="PLW22_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118304733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOU74396.1"
                     /translation="MTTIEQVKELAKRRDALRRYLDIDKLSIEYEEEQQKTQAPDFWD
                     KPDEAEKQLKKVASIKSWIDGFNKVNSLTEDLQVMFDFAKEGAATEEEIDSHYRQTLQ
                     AIEDLELKNMLRKEEDRLGAIMKINSGAGGTESMDWAAMLMRMYMRWGERNGYEVKVV
                     DVQDGEEAGIKSCTLEFVGDMAYGFLKGENGVHRLVRISPFDSNARRHTSFASVFVSP
                     AVDETIEITINPADIKWDTYRSSGAGGQNVNKVETGVRLYHIPTGIVIENTESRSQFQ
                     NKENAMRLLKSHLYELELRKRREIQAEIEGKKKKIEWGSQIRNYVLHPYKLVKDLRSG
                     YETSDVNAILDGDLNECIKAYLMQFGDE"
     gene            complement(12541..13497)
                     /locus_tag="PLW22_06530"
     CDS             complement(12541..13497)
                     /locus_tag="PLW22_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU74397.1"
                     /translation="MKNNSIGIYAATVLAMVFWSLSFIWYKEVFQFYKPITLVVGRLS
                     ISAIFLFITVGLIMRKLTKIKASDLKYFLLLAFFEPFLYFMGESFGMTMVSSTLAAVI
                     ISTIPLFSPLGEFYIYKTRVTLMNFVGIMVSVVGVGMVIFHQGFGKLEANPVGIALMI
                     LAVFAALGYSLTLRKLTSAYNVFTIITYQNTIAVIYFLPIFFLFEFNHFMSVGVTISS
                     LMPLVKLGIFASTFAFLLFTYSVKHLGVTKANSFANAIPVLTAIFAYFLLGETLGIVK
                     IAGIAIVVLGLFLSQVPREHARRVFAFIPVGLITRARLRKNE"
     gene            complement(13614..14957)
                     /locus_tag="PLW22_06535"
     CDS             complement(13614..14957)
                     /locus_tag="PLW22_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOU74398.1"
                     /translation="MKTRKNLFALLALVGLAGTLLLTSCEKDEEKEMEMPKNIVEVAV
                     SNPQFSILVQALQKANLVTTLQGTGPFTVFAPTNAAFNELFSQLGVSSIDALTADQLT
                     PILLYHVLSGKVESNQLASGYVSTLSPGAGGLGVSLKVDASMLKLNGNVGITAADISA
                     TNGVIHVIDKVLLPPTVVDIALANSSFTSLVAALTKANLVNALKADGPFTVFAPTNDA
                     FSQLFTDLGVSGLDALNAEDLTPILLYHVLGTAVKSTQLQTGYVSTLSAGPNDSKVSL
                     LVDAAAVKLNNNSKIVATDVVGTNGIVHVIDKVILPPTVVDIALANSSFSTLVSVLVK
                     AELVETLKGEGPFTVFAPTNDAFSALFTQIGVSGIDQLSKDDLTPILLYHVVSGNVRS
                     NQISSGNVSTLNGDINVNVGTTVTINENTSVVLVDVQATNGVIHVINKVLLPPAK"
     gene            15149..16294
                     /locus_tag="PLW22_06540"
     CDS             15149..16294
                     /locus_tag="PLW22_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU74399.1"
                     /translation="MEELIRLRHELHRNPELSGQERATGKRIRFFLSRYQPHELYSNI
                     AGEGMAVVYRGKEPGPTVLFRCDMDALPITELSDFKYKSNTPGVSHTCGHDGHMAIVS
                     GLAVRFFENPLEKGKVILFYQPSEENGSGAKESIERLKELDLMPDYAFALHNLPKYKK
                     GSVVLGRYTFTAASKGLIIKLTGRNSHAAFPELGLNPSAATAEIIQHLLVLPTNFKYK
                     NFVLVTLIHVKVGEVAFGTSAGSAVIMATLRAFDDSDMHLLSDNALKTSTQIATRHGL
                     AIETEFTDDFPASVCDPQLTNVVEKIASEQKKEVIYLNEPNRWSEDFAHFTGVCPSVI
                     FGLGAGEDHPDIHSPTYDFPDDLIIDGVDLLDSIAREITSSESFLKY"
     gene            complement(16263..17198)
                     /locus_tag="PLW22_06545"
     CDS             complement(16263..17198)
                     /locus_tag="PLW22_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU74400.1"
                     /translation="MVIAKNKRAEAHLAIILANIMFGLNYSIAKGVMPNYLSPMAFTL
                     LRVVTASFLFWLISIFDKSDERISKADYPRFIASGLFGISFNQLLFLNGLNYSTPIES
                     SIISTLNPAMVMLIAYFVLSEHINFVKVTGLLIGASGALLLILGKGSLEVGQNHTLGN
                     AMLFLNTLFYAFYLVVVKPLMLKYKPITVMKGVFVVGLISLLPFGVYDLATTRWAVIP
                     PMIYASILFVLLGPTFLAYLLNGWGLRYVQASTVSIYIYSQPVIAALVAVSVGMDSLD
                     AKKITAAALVFAGVYLVSQRPDGWLVSILKRIQRK"
     gene            17374..>18269
                     /locus_tag="PLW22_06550"
     CDS             17374..>18269
                     /locus_tag="PLW22_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU74401.1"
                     /translation="MHKQTAHIKENPAQRIKAKLALIPVLLGLGVTGVLLYNEFNPRA
                     FDVINFTWKSALWLMLAWLFMVGRDLGYTLRLMILSDGKFRFWKALRVIMLWEFTSAI
                     TPSAIGGTSVAIVYVNKEGLNLGHSSALVMITSFLDELYFVVMFPLLVLLAGYHGLFD
                     IGTTNSMAKELLTFSIIGYSLKLAWVITLVYGLFFNPRGLKWLLLLIFKLPFLRRWKH
                     GANRAGSDIINSSKEYRNKPTSFWVKVVLASFLSWSSRFLVVNALLMAFFSIEHNLLI
                     FARQLVMWIMMLVSPTPGGSGFA"
BASE COUNT         5244 a         4129 c         3888 g         5008 t
ORIGIN      
        1 aggccgaagg gctaagctca acctctagct gggtgcgaca ggcattccgg cttttgggct
       61 tatcggtaac cctggctagc gttacaaata tgtggttcaa cgtactgtcg aacctaaaga
      121 tggttacctc atcgccctta agctggccgc ttacggcgta acccttgccg gtttcaaagt
      181 gggtatcgag cttaaacgag gttaggtggt taaccggcgc agtacagtgg gtaagaatgg
      241 ctttgccatt gcccacatgg gcaacattgg ccatccatgg aatggcgttg catacctctg
      301 acgatagcat cattgcggca gcactgcaaa tatcggcctc gcaggctgtg ggtatgccat
      361 cgtagtttag cttggccaat gccaggcagg cggtatggcc ggttttatta accaacggaa
      421 aacattccac ggccatggcg ttaagcctgt aaaacggaat aagtgaggca agcccctcgt
      481 atatcttacc gctctcaatg agctccaccc ggttctcggc catgccaaaa agcgaggtaa
      541 agtccggcga aacctgcgat atctcatcaa aaacaatatc atcccagcta atatcaacct
      601 gctcaacccc caacttgctt tgtaaaaggt aggggttaac accgctggcc acaagccacg
      661 gcgatgagct gcccaccaca ccaagtcgct gaccgtgaag cttgcgtacc cccacaaagg
      721 cattgtagta ctcgctcagg tactcggcgg ttgaagggtt atcggcatcg agcagcacgg
      781 aacgtatgtt atgctggttc atccaggctt taacctcggt ggcagcagcc catgcattgg
      841 cttcgcgcga ggcgagcagc agataaaaac ggtactcctg caccgactca atggctatat
      901 gctcagagcc cccggtcaga aacataagca catcgggctt atcgttcaca aactccatct
      961 cctcggggtt aaacaaaccc atgtacttct ttttggcctt gtcgaaaact gaaagctctg
     1021 ctgccggaaa agcaagcaac tttaccctta acctatcctt aataaaatcg ttactactca
     1081 ttgttttcaa tgcttttaac aatactaatt ccgaacctga tatactggta aacatcctca
     1141 ggagtgccat cgatacggtt gttgttcctt acgtgtccca acctaaccac cacggcattc
     1201 aagctaggaa ttacaaagat atactgtccc agcaaaccgc gagcatacca ggtcttgtaa
     1261 ccatcaacat ttatcatcca ccactggtaa ccgtagaaat cgacattctt gttagtttct
     1321 gggtcaaaga ggtaacttgc cggtgtaagc gactcgttaa ggtattcttc cggcacaatc
     1381 tgttccccat tccaacggcc accgttcagc accagctgac caaagcgggc gaagtcgcgg
     1441 gcattggtgt taaagcaaca gaacgccttt tcaacgccat ccttgcggtc caaacaccaa
     1501 agggcatcgt gctcggcgcc aatctttgac catatctttt ccgaggcgta atcggccagc
     1561 gttttaccag ttgccttttc aataatgagc gatagcagct gtgtgtcgcc gctacggtac
     1621 tcaaaggtct ttccgggttc ctttaccacc gtttggttta gcaccagctt gggcaggttt
     1681 ttgccgtaat aaccctgcgt ggtaagggag aatagcgtgc tatacgattc gtcccagctt
     1741 agccctgagc tcatggtgag cagatgcttt atggtgattt ttgccttatc gccctcagca
     1801 aactcaggga ggtagttccc aactggctca tcgatacttt tcacataacc atcgcttagg
     1861 gcacaaccca tgagtaggct gacaatgctc ttggccattg aaaacgagtt ggagtatgaa
     1921 tcttttccgt atccatccca gtacgattca aaaagtaggg cggtatcctt tacaaccagg
     1981 aaggctactg tgcggaaatc gttaaagtat ttgagttggt cgttggtaag ctgataggag
     2041 ttgtactttt catgcaacct ccagggttga ggggtacccg ccttaacggt acggttatgg
     2101 aatattttgt agtcgtcgat atttacctgc tggtaaatta gtgcccgcac aatgtaatgg
     2161 ttctgaggca aagccaaata tactgcaagg gctacaacaa ccactacgct ggccacctta
     2221 agttttcgct tccagttcat agcatgctag gtttaggttg atatgttgtg tcgaaattaa
     2281 ggattgtttt ggattttttg tgatatttgt tctcaaaata tttcatttga cccgaatcat
     2341 gaaaagcata atgcttccaa tagcgcttat ttcaacattt attgccattg tttacaaatc
     2401 caatgctcaa ccggatagct cgggggtagg cctctacctt ttgggacaat cgttttttga
     2461 taacaaggag tttaccggga acatcaaaaa ggggtatacc caccccggct tttacataca
     2521 gccggccatc aaatttaaaa cggaacagta cagcattgca gcgggtttcc atactttata
     2581 ccttgccggt gccgactcgc ttgaacggtt ggtgcctgtt ttttcggcta gtttacagct
     2641 aaatagcagg gtaaccctaa ttgtaggaac attagaatcc aaaaacggcc actggctacc
     2701 cgagccacta tttaaacccg aaaggctatt cctgaaccaa cccgaaacgg gtgtacagtt
     2761 cttgtatcga gggactgaga gcaaagccga tttatggata aattgggagc gttacatcaa
     2821 aacaggtagc ccatttcagg aagagtttac tgtaggattt acacatctgt accaccgaaa
     2881 caaaaatacc ggattctcca atgcctttca tgcaatggct ttccaccatg gcgggcaaat
     2941 tgatagcacc aacctacccg ttcaaacaca cataaacctt ggagttattc ctgcatatag
     3001 cttccagccc ctggctaaaa cacgatttga attttcatca tcgctttact actacaaaaa
     3061 cctttcccca tcgtttacct cctattacag ctcaggcttt ggcctgcacc ctcaactcag
     3121 agcataccac aatagcacca ccatcgatat tggttactgg tactccgatg ggttcataaa
     3181 tccgcgcggc gaagagcttt ttgggtcaat atctacaatt aatcctacat tcaaccaagc
     3241 ggttcgcaca atgggtactt tctcaattga attccaaaag gtatttcggg aaagcttgct
     3301 gctgaacgca aatttcaagg catactacga ttttaagtcg ggattgctgg agtacagcta
     3361 cacgctacgg ctgatgtttg atggaaagct tacacgggtg aagtaaaacc ccgacctgtg
     3421 gtggtcgggt gacatccatc aattttaatg ttattccgtt aaactatgca ttgaactaaa
     3481 aatatctttt cctccctcct tgttaaaatg ccaggtcagg aaatgctatc ttgactaaaa
     3541 ttaactacaa ctgagattaa taactttatt ataagtgttt tatctgtcat aatgatatct
     3601 acaactataa attagaattt cctgatcaat gacttgataa actaaccgat gctctcggtc
     3661 aatacgtctt gaccataaac cagccaaatc atattttaaa ggttctggtt ttccaatgcc
     3721 ttcataagga tgcttttgaa tgtcttttat taactcatta atttttttga gcattttttt
     3781 atcctcattc tgccaagata tatattcttc ccaggaattt ttggaaaatg taattcgggt
     3841 cattcttcga taagtttgtt tcgaaccaca ttcccttcct tcatttgttt aattgactca
     3901 taaagtctgt cagcatttgc tttagaattt aacaagtgaa gagtttccat tatagagtta
     3961 tactcatcta acgacatcat aactgcgcct ttccccgttc ctctcttaat aataagggtt
     4021 tcattgttat tcacaacatt gtccagaaac ttctttaggt ttgttctaaa ctctgtaaaa
     4081 tttgcggcca tcataatctt ttccccttta agttactttt tcagtacaaa tataagtaca
     4141 tatatagtaa cttgcaagaa aatttactta ttttctttgg gacagtagat gttaaccatc
     4201 tgctcagctg gtttgtagcc cagctctaca gctttgcgga aatcgtcgca ccccgacttg
     4261 aactcctgaa cgttcatttt tgagataccg cggttatagt atgcctcggc attcttgggg
     4321 tcggccttaa tgataacatc ataatcgggg atggcatcct tatggttgcc cataattgcc
     4381 tttgaaacgg cacggttgta gtaagcctga acgtaattgg ggtcaatgga aatcaccttg
     4441 ttgaaatcgg taattgaacc cttgtaatcc ttttggaacg cacgggcaac acccctgccg
     4501 tagtatgctt taaggtaatc gggcttaagc tcaatggctt tattgaaagc aacaacggcc
     4561 tgcttgaact caccctttcg gagcaatgct ccacccttta ctccgtaagc caatggtaat
     4621 ttagggttaa ctgctatggc cttatcgagt accttaatgg catcgtcgaa tcgctgcaca
     4681 ttggcaaagg catcaccaag gctataaagg gcttcctcgt ccttggggag ttccttaagg
     4741 gctacctcca aatcggcaat tgccccattg taatcctgaa ttttgagttt aaagattccc
     4801 ctcttcttgt aggcctcagg ggtagcgtta gccccttcaa tcatcttatc gtaatcggca
     4861 attgctccct taaagtcggc aaggtttgcc agcgactcgc cacgactgta aaacgcctca
     4921 aggtttgagc catcgagctc aatgctgcgg ttatagtcct tgatggcttg ctcaaaatcc
     4981 atggttgcag cgtttaacaa cccacgctgg tagtatgcct ttgagctgga atcgttcatt
     5041 gctatagccc ggttcaggtc agtcatggcc tgcgaatact gctttagctt acggtacgat
     5101 actccacgtt caatgtacag ctcaaaatgg gttgaatcaa taccaatagc cgtactaaaa
     5161 tcggccaaag ccagcgagtc gttatttaat gcggcaaggg ttacaccgcg cttaaagaag
     5221 taatcgtagc gtttggggtt taacttaatg gctctgttaa acttttcaag ggcaagctca
     5281 tacttggccg aatcgagcag ctttaaacct tcattgtagc tgttttgtgc caaacggggc
     5341 ttaagcaaaa tgtaggctgc aaaagctatg ataaccagcc caacaagtat aaccacagta
     5401 cgtttcatac aaatctgctt taatatcaat tcaaatttat acgattattt tgatataaag
     5461 gttatttatt ggcttcaact atcaacgcgc agttgagttt agccacctcg cgcgcaaaac
     5521 gtgggttttg gtttgagagt aagtcggtta gcttgtggta gttggggtcg tcgtgctggc
     5581 ttatgaagaa aagcgatttg tagctcctaa ggtaaaagct atagctatcg ctatagtttc
     5641 ggtaggtatt gtcgttaacc gttttgagca gggtgtaggt tgaacagatt ttttgtgcat
     5701 gcttcctaaa atcaaccgaa ttgggataat caataacatt gaggtaccaa cccgttgaat
     5761 cggcaaccgg gcagaacgat accatatcgt tattgagcat gaatttcacc tagccaaagt
     5821 atcggataaa cggttggtca tatgctggct accatagagg ccaagttcct cggcagcaaa
     5881 ggttgcaaaa acaatggttg ttcggggtgc aaaacccgat tgcttaagaa ctctggcaat
     5941 ttcaagggtt accgcaacgc cgctggcgtt atcgtcggca ccgggagcca cgtcaagagg
     6001 gctttcgggt tcagtacttc gtaaaataca atcgtgatgc cccccaataa tggtaaggct
     6061 atcggggaac tcaacccctg gcaaagtaca aaccacatta tactgccagg tttcatagta
     6121 ctgatttcgg aaatatacgt taagctggaa tgaatcgaga cgggcagtag cgtatccgta
     6181 gcttttaaac ttattcatta ttgcaacggc tacatcgcgc ctgttttcgg ccagtgcaaa
     6241 acgtgtaccc attccctgaa gcatgccaat tgtagcagca atgctatcgg catggatttt
     6301 tttggttaac tgggcaattt caggatcggg cttactggtg gggtcaacaa tttcgtcgtc
     6361 ctttgaacag gacaccacaa tgccaaatag caatagggcc aacaacccct taaatacata
     6421 acgataggta gaagtcataa tctttagttt tgatgtaaaa ataaacattc tgttaattcc
     6481 gaaacgctcc caattcagaa aattgatacc tttggtggct gaaccctgaa attaaaaggt
     6541 ataacaaaac caattgcccg atggagaact tatcagtaag tcaattcaag aagtataaca
     6601 acctaatagg atggttcact tttgccatag ccacactggt ttacctgctt accattgaac
     6661 ccactgccag tttttgggat tgtagcgagt ttatagccag cggtaataag cttgaggtag
     6721 gtcatcctcc gggggcaccc tttttcatgc ttatggttcg tttctttact atgtttgcac
     6781 catccccgca atgggttcct atacttgcca atagcctatc ggccctagcc agctcattca
     6841 caatcctgtt ccttttctgg accataacct acatggctcg gcgaattgtt tcgcccaaag
     6901 caactgagtt caccttaacc caagtaatac tcatactttc atcggggttt gttggcgctc
     6961 ttgcctacac cttttccgat acgttctggt tctcagcggt tgaaggtgag gtgtatgcca
     7021 catcatcgct ctttacagcc cttgtttttt gggcaatcct aaagtgggaa gagcaagccg
     7081 atcagcccca tgccacccga tggatcattc ttattgcctt tttaatgggt atttccattg
     7141 gaatacactt actcaacctg ttggcaattc cggccattgc tcttgtttac tatttcagga
     7201 aatacagcta ctcgcctaaa ggcctatttt acaccctttt aatttcaggt gtgctgctaa
     7261 ttaccataat gtatggtata attcagggat ttgttgtgat agcttcaaag gtagagttgc
     7321 tttttgtaaa ttcctttgga ctaccaatta aatcgggtgt tttcttcctg tttaccgttg
     7381 tactggcgct gcttgcttac ggtatttata gaacacataa gcaaggccgc gtggtgctga
     7441 atacaatttt tgtagggctt acggtgataa tgattggcta cagctcgtat gctgcaatag
     7501 taattcgctc gtcggccaat ccgcctatgg atcagaatag tcccgataac atgttctcac
     7561 tactatacta ccttaaccgt gaacagtatg gcgatagacc tttgctgtac ggtcaggtat
     7621 tcaatgcaca gcccatagca agggttgagg gcgagcctac ctatgcgcca aagaatggta
     7681 agtacgtttt tgttcgcacc aaggataaat acgagtttga ccctgagttc aaaatgctct
     7741 tcccccgtat gtggagcaac gaacaaaacc atgttaacgc ttacaagaaa tggagcaact
     7801 ttgatggcaa ccccattaaa cgctttgggc ccgatggcaa aaccgaaacc atatacgtgc
     7861 ccacctttgg cgagaactta ctcttcttct tccgatacca gcttggcttt atgtacttcc
     7921 gctactttat gtggaacttt gctggccgcc aaaacgacct tcaggggcat ggcgaggtaa
     7981 ccaagggtaa ctggataagc ggaatccctt tcatcgataa cccacgttta gggccgcagg
     8041 aaaagctgcc tgaaatttac cgtaacaata aagggcgaaa ccaatactac ctactccccc
     8101 tgctcttagg tttagccggt ttattgtacc atcttagtag gcataagcac gatttttggg
     8161 taaccaccat gctttttgtg atgaccggaa tcgctatagt tgtttacctg aaccaaacgc
     8221 cctaccagcc acgtgaacgc gactatgcct atgcgggaag cttttacgcc tttaccattt
     8281 ggataggttt aggtgtttta gcagtttatg aacttctgaa aaagataaaa gatacaccag
     8341 ccacggctgt ggttacagca tcggtatgca cgctggccgt acccgtacta atggccagcg
     8401 agaactggga cgatcacgac cgctcaggga gctatgtgcc agttgatttt gggtataaca
     8461 tgcttattgg ctgcaaaccc aacgccattc ttttcaccta tggcgataac gatactttcc
     8521 ccctatggta ctcacaggag gtggagggcg ttagaaccga tgttcgtgtt gctaacctaa
     8581 gctacctgcg cggcgagtgg tacattgaac agatgcagcg taaagcttac cagagcgacc
     8641 ccataccaat ccgaatgaca tacgaccaat actttaccgg cgctcgcgat gcaattgtgg
     8701 tgattgatag gataaaagaa cccctgaacc ttgaccgtgt ggtggatttc ttcctttctg
     8761 acaaacccga aaatatgatt caatcaccat ttaaccaaaa cgaaaggata aactacatcc
     8821 cttccaaaca gcttttccta accgttgata aatcagaggt taaaagcaaa gtcgatttgc
     8881 cggacaacta cctaaaccgc attgccgaca ccatgacctg gacacttaac aagcaggttg
     8941 tgcttaagga cgggcaggta atccttgacc ttattgctac aaaccaatgg aggcgtccca
     9001 tttattacgc cacaactgtt agcggtgaaa cctatttagg tttggataag tatttccacc
     9061 tggagggtat gatgtaccgt gtactaccta ttcctgcctc gcggcagtgg gggactggtt
     9121 cggttaacac ccgcgagatg tgggaaaacc tcatggaaaa gtataggttc aggagcttga
     9181 acaaccccaa ggtctatctg gacgaaaaca agatgcgaat tgtatctaac taccgtaacc
     9241 tttttgctcg ccttgccaat gctttagttg atgaaggcaa aatggattcg gccatcactg
     9301 cactgaacag gtgcatggag ctaattcctc ctaaaaatgt tccgcttaac tactttgccc
     9361 tttccatggt tgaaggctat taccgtgcag gcgatatgca aaatgccctg aaatactctc
     9421 agcaaatggt aaaccaagcc tccgaggcca tatcgtacat gatttatgat ctggacgagt
     9481 ttcaaaaaaa ttggcttgga aacgagcttc agctaaatct tgcaatactt tatgagttaa
     9541 cacgaatggc caaccagtac gagaagggcg agtacgctaa acagctaact gataagttca
     9601 actcatacgt tacggttctt gaagggtagt tatgataaat cttagtcccc cgcgtttttt
     9661 tcagaggttc ttcaggaatg tgatttggaa tttcccctcc gaaaaggaag gggttttcct
     9721 cacgttcgac gatggtccaa cgccgggagt taccccatgg gtgctggagc agctgcgcaa
     9781 gtacaatgca aaagctacct ttttttgcct ggcaaaaaac attgagatgc accctgaaat
     9841 ttttgagatg attgtggccg atagacatgc tgtgggaaac cactcctaca gccatatcaa
     9901 gggatgggga accgacacca ctcagtatgt gcaggatatt atttacgcca acagctttgt
     9961 aaacagtaac ctctaccgcc caccctacgg gcgtataacg cccaaacagt tcaagctgct
    10021 aaaatcgcaa tacaagatta ttttgtggga tgtgcttagc atggactaca gccgaagggt
    10081 ctcacccaaa agggtttacc agtacgtagt tcgaaatgcc aaacccgggt caatcattgt
    10141 ttttcatgat tccttgaagg ctgagcgtaa cctgcgctac gcactgcccc gcgtactcga
    10201 taatctcagc tctaaagggc taacctttaa atcgattatt attccatgaa cagaataatt
    10261 agatgagtaa tatactcttt attgttcaac ttccgcctcc ggttcatggt gctagtttaa
    10321 tgaataagct ggtaatgggt agggcttcgc aattgccaaa caacaaatgc tttaccatta
    10381 gccttaactt ctcaaaatcg ctttccgatt tacagcggtt taaaccccgt aaaatactta
    10441 gggccataac ggttgttttc gaaattattg gtaaaatcat taagtacaag cccgatatag
    10501 tttacttctc aatggttccc attggacttg tgttgattag ggatagcttt tatttaatgc
    10561 tttgcaaggc attatcacca aaatcaaaaa agataattca tcttcaccgg ccgggaatgc
    10621 tacagtttta tagtcaaaaa ccatacctgt accgattcta tagctttatt ttcaaaaact
    10681 gcgaaattgt tcacctaacc cctcgcctgg ccaggttgga gctttcccct ttgaacttaa
    10741 aaaaaactaa aattaccgtt atacccaact ccataatcaa aacacccgaa tacgaaaggc
    10801 acgaaaagga ctggagtaat atcctattcc tttcaaattt tctgatacat aagggacatt
    10861 ttgagctagt tgaagcattt gcaatactca gcagcaaata cccacagctt aagctcaccc
    10921 ttgcaggggc attcccatcc gaaacagaag agcaaaactt gctggaacga atcaaacagt
    10981 atgggttgaa cagaacggtt gaggttgtag gccctgttta cggtcccaaa agatttgaac
    11041 tgtatagtag ggcaggtatc tttgtacttc catcaaaact agagtacttc cccctggtta
    11101 ttctggaagc catgtcggaa cgatgtgcag ttataaccag cggaaaggaa aatttgcagg
    11161 atacctttgt tgatggcaag caactgcttt tccttgaaag cgtagacccc tacgatattt
    11221 cgcagaagat tgaaagatta atacaatcac cccagcttac ccaatcaatt gcgaacgaag
    11281 gctacaaaag gttcaccgaa atacaggaag aaagcctctt aaaaattgac aagctcttca
    11341 cctgccaata gagtttgatt gtactttcaa taattttcat gattgccttc aatcctaggg
    11401 catctaccat aaaatctata tctttgcaaa aatcttttta cggaatgaca accattgaac
    11461 aggtaaaaga gcttgcaaag cgccgcgatg cgctgaggag gtatctttga catcgacaag
    11521 cttagtatag aatacgaaga ggaacagcaa aaaacacaag cccccgactt ttgggataag
    11581 cccgatgaag cagagaaaca gcttaagaaa gtagcttcaa tcaaatcctg gatagatggc
    11641 tttaataagg taaacagcct taccgaggat ttacaggtta tgtttgactt tgccaaggaa
    11701 ggtgctgcaa ccgaagaaga aattgactcc cactacagac aaaccctgca agccattgag
    11761 gatttggagc taaagaacat gctccgtaag gaagaggata ggcttggggc aatcatgaaa
    11821 atcaactccg gtgccggagg aactgaaagt atggactggg cggccatgct catgcgcatg
    11881 tacatgcgct ggggcgagcg gaatggctac gaggtaaaag ttgtagatgt gcaggatggc
    11941 gaggaggccg gcataaaatc atgtacattg gagtttgtgg gcgacatggc ttacggattt
    12001 ctaaagggcg agaatggcgt tcaccgcctg gttcgcatat ccccattcga cagcaatgcc
    12061 cgaaggcaca cctcgtttgc atcggtcttt gtttcgccag cagttgacga aaccattgaa
    12121 ataaccatta acccagccga cattaaatgg gacacttacc gttcatcagg ggccggtggc
    12181 caaaacgtaa ataaggttga aacaggcgtc cgcctttacc acatccctac gggcatcgta
    12241 attgagaata ccgaatcgag gtcgcaattc cagaataagg agaatgccat gcgactactg
    12301 aaatcgcacc tttacgaatt ggaactacgt aaacgccgtg agattcaggc cgagattgaa
    12361 gggaaaaaga aaaaaattga gtggggctca caaatccgga actatgtgct ccacccctac
    12421 aagctcgtta aggacctacg gtcgggctat gaaacctccg atgtaaatgc catactcgat
    12481 ggcgacctta acgaatgtat taaagcttac ctgatgcaat ttggcgacga gtagccaatg
    12541 ctactcgttt tttcttagcc gggcacgggt aattaaaccc actggtataa aggcaaaaac
    12601 acgccgggca tgttcccttg gtacctgcga aaggaataat ccaagcacta caatggctat
    12661 tccagctatt tttacaattc ctaaagtttc acctaacagg aaataggcaa aaatggctgt
    12721 aagcacagga attgcattgg caaacgagtt tgccttggtt accccaagat gcttaaccga
    12781 gtatgtaaat agcaaaaagg caaaggttga tgcaaaaatt cctagcttaa caagcggcat
    12841 tagcgaggaa atggttactc caacgctcat gaaatggtta aactcaaaca agaaaaatat
    12901 tggcagaaag taaattacgg caatagtatt ctggtaagta attattgtaa aaacattgta
    12961 ggccgatgtc aacttcctaa gggtaagtga atagcccaat gcggcaaata cagccagaat
    13021 cataagggct ataccaacag ggttagcctc aagtttccca aatccctgat ggaatataac
    13081 catgccaacc ccaacaaccg aaaccatgat acccacaaaa ttcattagtg ttactcgggt
    13141 cttgtaaata taaaactccc ccaaggggct gaaaagtgga atggttgaga taattacagc
    13201 agctaatgta gatgaaacca tggtcatccc aaaactctca cccataaagt aaaggaaggg
    13261 ttcaaaaaag gctagcaaaa gaaagtattt caaatcgcta gctttaattt tggtcaactt
    13321 cctcattatt agtcctaccg tgataaaaag gaaaatggca gaaatagata gcctaccaac
    13381 caccaaagtg ataggtttat agaattggaa tacctccttg taccaaataa aggagagcga
    13441 ccaaaaaacc atggccagca cagttgcagc gtaaatccca atggaattat tcttcattgt
    13501 ttaaagttca atttattgac aaaggtttag aacggtataa acccaaaagg tttacgcgac
    13561 aaaaataaaa aaagggcgtt gaacactcaa cgccctaatc taataatgca agcttatttt
    13621 gcgggaggca aaagcacctt gttaattaca tgaataacac cattggtagc ctgtacatca
    13681 accagaacaa ccgaagtgtt ctcgttaatt gtaacggttg taccaacatt aacattgata
    13741 tcgccattca atgtagatac attaccacta gaaatttgat tcgatctcac attacctgat
    13801 actacgtggt agagcagaat gggcgttaaa tcatcctttg aaagctggtc gattcctgat
    13861 actccaattt gagtgaaaag tgcactgaaa gcatcgttgg ttggagcaaa cactgtaaat
    13921 ggaccctcgc cctttaaggt ttcaacaagt tcagctttta ctagaaccga aacaagagtg
    13981 ctgaaactgc tattagccaa tgctatatca acaaccgttg gtggaagtat aaccttatcg
    14041 ataacatgaa ctataccgtt tgtaccaacc acatcggtgg caacaatttt agaattgttg
    14101 ttcaacttaa cagcagcggc atcgacaagt aaggaaactt tagagtcatt tggacctgcg
    14161 ctcaaagttg aaacatagcc agtttgaagc tgtgtggatt taactgcagt acctaaaacg
    14221 tggtataaaa gtattggagt caaatcctcg gcattaagcg catctaaacc acttacacct
    14281 aaatcagtga aaagttgact aaatgcatcg tttgtaggag cgaaaactgt aaacggtcca
    14341 tcagccttaa gagcattaac caggttggcc ttggttagcg cagccactaa agatgtaaaa
    14401 ctgctgtttg caagggcaat atcaaccact gttggcggaa gtaaaacctt atcaattaca
    14461 tgaataaccc cattggtggc tgaaatatcg gcagcggtaa taccaacatt tccgttgagt
    14521 ttgagcatac ttgcatcaac cttaagtgaa acacctaatc cacctgcacc ggggctaaga
    14581 gttgaaacat agccactcgc aagctgattg gattcaacct tacccgataa aacatggtaa
    14641 agcagaattg gggttagctg gtcagcagtt aaggcatcta tactactcac gcctaactga
    14701 ctgaagagct cgttgaaggc ggcgttggtt ggagcaaata ctgtaaaagg accagttccc
    14761 tgaagggttg taaccaagtt cgccttctgg agggcttgaa ccagaattga gaactgtgga
    14821 ttggaaacgg caacctcaac aatgttttta ggcatctcca tctccttttc ttcatccttt
    14881 tcgcaacttg ttaatagtag cgttcccgct aggcctacca aagctagtag agcaaataga
    14941 ttttttcttg ttttcataat tgtgtgtttt agttgattat gccctactaa cattaaaaaa
    15001 tagtctttgt tcatttagta ttctatttac atattctttt gttggttaat ttttgttaat
    15061 tatatatctt ttttttgatt gttttatttg gctggatttg gatttattta ccaaaaatcg
    15121 ataattttac agaaatttgg ttgcaggtat ggaagaactc atcaggctac gtcacgagct
    15181 gcatcgcaac cctgaactct ctggccaaga aagagctacg gggaaaagaa ttcgtttttt
    15241 tctaagccgc taccaaccac atgagcttta ctcaaatatt gctggtgagg gaatggctgt
    15301 agtatacagg ggtaaagagc ctggccctac agtactattc cgctgcgata tggatgccct
    15361 gcccataact gagctctccg attttaagta caagtccaat actcccggag tttcacatac
    15421 ctgtggccac gatggccata tggccattgt ttcaggcctg gcggttcgtt tttttgaaaa
    15481 tcctttagaa aaggggaagg tgattctgtt ctatcaaccc tcagaggaga atggttccgg
    15541 tgccaaggaa tccattgaaa ggctaaaaga gctagacctc atgcccgact atgcatttgc
    15601 acttcataat ttacccaagt acaaaaaggg cagcgttgtg cttggccgat acacctttac
    15661 ggcagcctca aaaggcttga tcattaagct taccggacga aactcccatg cagccttccc
    15721 agaactggga cttaatccat cggcggctac agctgaaatc attcagcacc tgctggtatt
    15781 gccaaccaat tttaagtata agaactttgt gctggtaacc cttatacacg ttaaggtagg
    15841 tgaagtggcc tttggaacct cagccggttc ggctgttatt atggctacac tcagagcctt
    15901 tgacgattcc gatatgcacc tactttccga taacgctctc aaaacatcaa cccaaattgc
    15961 aaccagacat ggcttagcca ttgaaaccga atttaccgac gatttcccag cctcagtttg
    16021 cgaccctcag cttactaatg ttgtagagaa aattgcctca gagcaaaaga aagaggttat
    16081 atacctcaat gaacccaacc gctggagcga ggattttgcc cactttacag gggtatgccc
    16141 atcggtaatt ttcggacttg gcgcgggtga agaccacccc gatatacata gcccaaccta
    16201 cgattttccg gatgacctta ttattgatgg ggttgaccta ctggattcaa tagcacgtga
    16261 aatcacttcc tctgaatcct ttttaaaata ctaaccagcc agccgtcagg acgctggctt
    16321 actaagtaaa cccctgcaaa taccaaagct gcagcagtta tctttttggc atcgaggcta
    16381 tccattccca ccgatacagc aactaaggcc gctataacag gctgagagta aatatatatg
    16441 cttacagtgc ttgcctgaac gtaccgtaat ccccatccgt tcaggaggta tgctaaaaat
    16501 gttgggccaa gtaaaacaaa aagtattgaa gcgtaaatca ttggtggaat aactgcccac
    16561 cttgtagtgg ctaaatcgta aacaccaaaa ggaagcaggg aaatcaaacc aaccacaaaa
    16621 acacctttca taaccgtaat tggtttatac tttagcataa ggggctttac taccacaagg
    16681 taaaaagcat agaaaagtgt attcaggaaa agcatggcat tgccaagggt atggttctgc
    16741 ccaacctcca aggagccctt gcccaggata agcaaaaggg cacccgatgc tccaataagc
    16801 aaacctgtta ctttaacaaa attgatatgt tccgatagga caaagtatgc aataagcatc
    16861 accattgcag ggttcaaggt tgatatgatt gatgactcga ttggggtact gtagtttaac
    16921 ccattgagga atagcagctg attgaatgaa atgccaaaca accccgatgc aataaatcgt
    16981 ggataatctg ctttgcttat tctttcatca ctcttgtcaa atatcgaaat caaccagaaa
    17041 agaaacgagg ccgtaaccac ccgaaggaga gtgaatgcca tgggcgatag gtaattgggc
    17101 ataacccctt tggctattga gtagttaagc ccaaacataa tgtttgcaag tattatggct
    17161 aaatgtgcct cggctcgctt attttttgca atcaccatat acattaaaaa tacaatgcag
    17221 ttcgatttct aaaacaaagt aaagtttaaa aatgcaaaac ttacagaagg gttaaaaagt
    17281 tcatgttttt aacccaataa acaatttagc ttttaattag ccatgattta aaccctaaaa
    17341 ctttaaattt gttaattatt gcatttgaaa ttaatgcata agcaaaccgc ccatattaag
    17401 gaaaaccctg cacaacgtat aaaggcaaaa cttgccctaa taccggtttt gcttgggctg
    17461 ggggtaacag gagtcttact atacaatgaa tttaacccta gggcttttga cgttataaac
    17521 tttacatgga aatcagcgtt gtggctaatg ctggcttggc ttttcatggt tggccgcgat
    17581 ttgggttaca ccctccgact aatgatttta agcgatggta aattccgatt ctggaaggcc
    17641 cttagggtga taatgctctg ggaatttact tcggcaatca caccatcggc tataggtggc
    17701 accagcgtgg caattgttta tgtcaataag gagggtttaa atcttgggca tagctcagca
    17761 ctggtaatga taacatcgtt cctggatgag ctttactttg tggtaatgtt tccactcctg
    17821 gttttgctgg caggttacca tggattattc gatattggta ccacaaacag catggccaag
    17881 gagcttttaa cattcagcat tattggctac tcattaaaac ttgcctgggt aataaccttg
    17941 gtttacggtt tattctttaa cccccgtgga cttaaatggt tgttactttt gatttttaaa
    18001 ctaccattcc tgcggcgatg gaagcatgga gcaaatcgcg ccggctccga tatcataaac
    18061 agctccaagg aataccgaaa caagcccact tcgttctggg taaaggttgt actggcctcg
    18121 tttctctcgt ggtcatcgcg tttcctggtt gtgaatgcgt tacttatggc attctttagc
    18181 attgagcaca acctgctaat ttttgcacgc caactggtaa tgtggataat gatgttagtg
    18241 agtccaactc ccggaggtag tggctttgc
//
LOCUS       DAOOWJ010000051        18238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3070_length_18238_cov_9.346807, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000051 DAOOWJ010000000
VERSION     DAOOWJ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 18238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18238
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3070_length_18238_cov_9.346807"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..2021)
                     /locus_tag="PLW22_06555"
     CDS             complement(243..2021)
                     /locus_tag="PLW22_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU74402.1"
                     /translation="MSERKLLKSGEFLVNEIDANDIFIPEEFNEEQRMIAQTCRDFME
                     AEVFPRLNDIDKGDRELMRSILQKSGELGMLGIAVPEQYNGFGQNFVTQMLVAETTGA
                     GYSFSVAYMCHCGIGTMPILYYGNEEQRQKYVSRLATGELIGSYCLTEPGAGSDANSG
                     KTTAKLSEDGKYYILNGQKMWITNAGFADTQVVFAKVDNDRVLSAFIVESKWPGVVIG
                     PDEHKMGIKGSSTAQIYYNDVKVPVENMLGNRGEGFRIALSILHMGRMKLGANVIGAA
                     KETITQSVQYANERKQFNTKIANFGAIKHKLAEMVIRAYAHESAIYRVSQDIDDLIEK
                     YKAEGCEYGRAAIDAISHYAVEDAILKVNGSEMLDFVVDEGVQIHGGMGYSAEMNVER
                     GYRDSRINRIFEGTNEINRLLVIDTAIKRSLKGDYDLMGPAEQLYNSLDSIVAEAGSS
                     DYYDQKMQTIRNFKKVAMLGIYGANKAFGKQFATEQEVQNNLSNIIMDLYVAESLALR
                     VKKLESKGHQNINIYKDIVDVFVYDAAGRIRKNAMDAANSFAAEPELSKLTAAIDRLC
                     KAPSVNVKDARRRIADKLIEDNEYKF"
     gene            complement(2046..2840)
                     /locus_tag="PLW22_06560"
     CDS             complement(2046..2840)
                     /locus_tag="PLW22_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOU74403.1"
                     /translation="METRKIEIPVMADLFARGEKPEYLFWVGCAGAYDDRYKKVARAF
                     AKILVHLNVSFAVLGKEETCTGDPARRAGNEMLFQMQALQNIELFKAYEVKKVITICP
                     HCYNTFKNEYPDLGADFEVISYIDILNKYIDEGKLSIDTDKFKSAKITYHDPCYLGRA
                     NGIYEQPRAILKKLGGELVEMERNRSFALCCGAGGAQMFKEAEHGNKEVFLERMDDVL
                     KVDPNIIATACPFCMTMMTDGIKYKNQEEKMRNLDIAELIAESLNL"
     gene            complement(2885..4204)
                     /locus_tag="PLW22_06565"
     CDS             complement(2885..4204)
                     /locus_tag="PLW22_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU74404.1"
                     /translation="MTKQIVFAIALLVTIGVFAYTISRIVRFFMLTRKGFAVSKLGKR
                     LWVTLEVAFGQTKIFRRPVIGLMHALVFWGFCVILFGSIEMVIDGLFGVEKSLSVLGA
                     FYNFMMATGDIFALLVAISIVVFLIRRVFLHIKRFEGIEMKKISHIDANVALSIILVL
                     MLSLMGMNAAYLKWVELAGHEVHGVYPVSQHLTWLFNGMSVDTVFTHYQIYWWTHILL
                     IFLFANLLPYSKHFHVFMSVPNVFLSRLEPLGKLPNMDNVTREVKLMMDPNAAFAAPA
                     TDAPIERFGVKDAEDVVWKSYFDALSCTECGRCTSVCPANITGKKLSPRKVMMDLRAR
                     MKEKGPMMLKNGRDYTDGKSLVRDYISEEELWACTTCNACAQECPINIDHPKLIVDMR
                     RYLVMEEGSAPGELKAMFNNIENNGAPWQYSPEDRLVWAKDLEINVQ"
     gene            complement(4333..5298)
                     /locus_tag="PLW22_06570"
     CDS             complement(4333..5298)
                     /locus_tag="PLW22_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOU74405.1"
                     /translation="MSVLVYTENWDGKFKKLSFELVSYAGKLAEMMGTTTTAVTIGEV
                     SNDELKQLGKYGADKVICLKSDNLKNLDSQLYTSAIAQVAQSEGSKVIVIANNNSGKA
                     IAPRLSVRLKAALGSGVSQLPLSLSPFTVYKRAFSGGAFAQVVLKTDIKIIALTQNSF
                     DIVENSKDVKIERVDIQVDAPKTVVKDVQKQTGKLLLTDAEIVVSGGRGMKSPDNWGP
                     IVELAELLGGATACSRPVSDEGWRPHDEHTGQTGKIIAPNLYIAVGISGATQHIAGVS
                     GSKYIVAINNDKSAPIFEVAQYGIVGDAQKVLPKLVEAVKEIKGK"
     gene            complement(5324..6073)
                     /locus_tag="PLW22_06575"
     CDS             complement(5324..6073)
                     /locus_tag="PLW22_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOU74406.1"
                     /translation="MKILVCISHVPDTTTKVKFVDGDTKLDTNGIQWVINPWDELSLT
                     RALELKDDASTGVKSVTVAHVGNATTEPTIRKALAIGADDAIRVNAEPTDAYYAAAQL
                     AEVVKKEQFDIVMCGIESSDYNNSAVGSMLAEFLGIPSVSAVSNIKVENGQAIITREI
                     DGGKEVLTVPTPFVAIVQKGIAKEPRIAAMRGIMMARTKPIKLYEPVAVDALTEYVKF
                     EMPKPRAACKFVDPENPKELITLLQNETKLI"
     gene            6476..6955
                     /locus_tag="PLW22_06580"
     CDS             6476..6955
                     /locus_tag="PLW22_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74407.1"
                     /translation="MSNEVIHSLIRNLDEKLSYSLVQLEKVSQEVTVHLQNNPEHSSD
                     ELVPALIRHFDIRRQSISYLKLMLKLRSFLNNDSKYILNFEKSSGIRIDCISEQMIIN
                     EKMPTYLSNNLNVDALNMEVYTSKAYQRYFEETYNELMRADRFEACSILETMKFSYN"
     gene            7310..7636
                     /locus_tag="PLW22_06585"
     CDS             7310..7636
                     /locus_tag="PLW22_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU74408.1"
                     /translation="MLMVKQIHKRTPQEKERILLDIQHLGVTAGCRKHGISRVLYYDW
                     LNRYQASGLEGLEDRRGKSVDARIKRLEKENRLLKELLAEKELESKLKDELLKKKFGQ
                     QTRNGR"
     gene            7684..8685
                     /locus_tag="PLW22_06590"
     CDS             7684..8685
                     /locus_tag="PLW22_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOU74409.1"
                     /translation="MAGMKRSTYYYRGKGSRKGKRPSTHTVKGGVMVPNEAVVQEIAR
                     LVEPEYHDYGYQLVTELLRQMGYTINKKKVYRLMEEGNLLNPPVRRGAPRSRAFIKYA
                     VPPLEGPFRTVEADIKYVHIHEENRNAYLVTFLCTFCRYAPVWDLQYSMKSKDIAELV
                     LDLVNDPEVKRFMGNQRVKIVIRTDNGPQFIARALAEILESLGIAHEFTMPGTPQQNA
                     HIESFHSTVTRLVCNRNVFRDIDHAREIFRAFYYAYNETRAMKSLLSYPPRKFLRLWE
                     AGIVGIRRDKRNKDFFFFREKPFPPTGIGSSPEDLIWANKNSIFDSSVLNPLEISPV"
     assembly_gap    8811..8910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9100..11010
                     /locus_tag="PLW22_06595"
     CDS             9100..11010
                     /locus_tag="PLW22_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOU74410.1"
                     /translation="MGKFEDILKQYWGYTSFRSIQLDIIESVYNGNDTLALMPTGGGK
                     SITFQVPALAMEGICLVISPLIALMKDQVENLKKRGISAVAIYSGMTQHEIDITLDNC
                     IYGEVKFLYVSPERLSTAIFRERIRKMRVCMVAIDEAHCISQWGYDFRPSYLKIAELR
                     SILTEAPFLAVTATATPEVVNDIQEKLHFRNGKVFRMSFARDNLVYLVRNVEDKEKYL
                     FKIVDNIPGSGIVYVRNRNKTKEIALMLQRAGYAADFYHAGLTMEMRNAKQNDWQTGK
                     TRIIVATNAFGMGIDKPDVRFVVHIDLPDSPEAYFQEAGRAGRDQKLSYAVLLYNESD
                     SLRIEQRLEANFPPPDEIKRIYQALGSYLNIAYGAGKGEVYDFNLIDFCSTYKLNMVV
                     AYNSLKILEQEGYIELTDELDNPSRIMFLVNKQELYRYQVAHAEMDKFIKVLLRLLPG
                     VFSQYVRIDEQFLSKTTGLPADKVYENLKTLSRQKILNYIPKKRTPLVIFIEERLDDK
                     NLRIEPERYQTLRERYRARMNAMLQYAQSQAKCRSQFLSEYFGQTDSYRCGKCDVCTA
                     RNELDMSKYEFDNILEKIKATLTKTPLPLENLIDSTGSNPDRVARVIRFLQDTGKITV
                     NCQGLLEWVIRK"
     gene            11340..12170
                     /locus_tag="PLW22_06600"
     CDS             11340..12170
                     /locus_tag="PLW22_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU74411.1"
                     /translation="MNERKIDIKNKLDGGLTFKISRFKEQIKKTKPHKHEDYYELIFL
                     SQGEGFHCIESEKFMVSSPDFYFLKPGQLHYWQFTSIPKGFVILFKDTEFNAVKENVL
                     LDLISKLKDTTRLTFQADKYPSYLLEEIFNEFQINTQYSKEIIHGLLKALFGKLLQIK
                     AENPNSPNQPQPIYERFVQLLVKECPRLHKVNEFADLLNVTPQNLNIICRKQTGKSAS
                     EIITNQLLLEAKRYILHTDNTINEIAEILSFSDASNFVKFFKKHEGITPIQFRDKYFQ
                     "
     gene            12253..13026
                     /locus_tag="PLW22_06605"
     CDS             12253..13026
                     /locus_tag="PLW22_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOU74412.1"
                     /translation="MEIVIICLAAFFTAVLTFFSGFGLGTILAPVFAIFFPIDIAIAL
                     TGVVHFSNNIFKIALVGKNSDKTVLLRFGIPAILSSFAGAWLLMKITVLPTLLQYELW
                     GRTFEVTPVKLIIAILLIVFSLLEISPSFQKVQFGKDKLIWGGLLSGFFGGLSGIQGA
                     IRSAFLIKIGLSKEVYIATGVVIACLVDFTRLSVYASRFISSTLHENLTLLISATLAA
                     IAGAYFGKRLLKKVTFRSIQLVVAVMLILISIALGAGLI"
     gene            13139..14446
                     /locus_tag="PLW22_06610"
     CDS             13139..14446
                     /locus_tag="PLW22_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU74413.1"
                     /translation="MAYSTDSAFLKQLRNTNLGNLIVWSYWWPAIVISAIFLGRIWCM
                     VCPVELITTFFAKIGLKRKRPAWLLSGWAITVFYIIILFIGIQGFAIHRNPFFMAVYL
                     LAIVGVSVIIGSIYEKNTFCRYVCPVGYLLGLYSRLSFFGWRVKDKNICNSCKDKSCI
                     HKKYQYNLNYKSCGVDLYPANITDNSDCILCAGCLKTCSKYQSEPNAERPNPQFTYIG
                     FANGLFQMKPLKMAELIFVLVVSGFVISEIWSEWKITDAYLSFLPDLVVKPLAIENKF
                     GSGFLKGTILFAFVPFILWLLPFLFARLAGVAIKFKAYLVNYGIAFIPIIAAAHLAKA
                     VLKSTSRIPYFEYLFDDFTGVSTAQKIIEGNITLQQTPPWLNTLVSLLLIGFMLIGVW
                     LSIKVVKHLNVKNGKEKSSKSYYLIPILYGGVFLIMLLVWRWF"
     gene            14622..14855
                     /locus_tag="PLW22_06615"
     CDS             14622..14855
                     /locus_tag="PLW22_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74414.1"
                     /translation="METLNSSELRQRLYDYSNQVGFDTQKDSFREVISFLIDIDQNFL
                     YTLLNPEEVRYLATHRDVEERLKRQLEQVVESL"
     gene            14936..17044
                     /gene="recG"
                     /locus_tag="PLW22_06620"
     CDS             14936..17044
                     /gene="recG"
                     /locus_tag="PLW22_06620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOU74415.1"
                     /translation="MGNILDSEIMYLPGVGPKRAELLQKELNIYTFGDLITFYPFRYI
                     DRTKINKISEVTDELPYVQVVGRVISISEAGVGRHRRLTAIVTDGTGRMELVWFQGIT
                     WVLKRLKPNVDYLIFGKATRFGAHFSIAHPEMEIYNPQQDVITGSLQPVYSTTEKLKN
                     NHITSKTIMGFVQTLIPMAIGKITETLPPFIIEQNKLMTLPEALRNIHFPANTTLLHK
                     AQHRLKFEEHFYIQLSILSRQKVRQTRVNGHVFSHVGEMFNRFYHERLPFPLTEAQKR
                     VIKEIRRDMLSGKQMNRLLQGDVGSGKTVVALMCMLLAVDNGYQACIMAPTEILANQH
                     YESITALMGDLPVKVGLLTGSTRRSERNNLHQSLEAGELNILIGTHALLEDVVKFKNL
                     GFVVIDEQQRFGVMQRAKLWEKNNDPPHVLVMTATPIPRTLAMTLYGDLEVSVIDELP
                     PGRKPVKTLHYTDAKRNLVFALMKEQIKRGRQVYVVYPLIKESEKLENLKDLEDGYES
                     IVRAFPPPEYVTVVVHGQMKPEDKDYSMNLFAQGKAHIMVATTVIEVGVNVPNATVMV
                     IESAERFGLSQLHQLRGRVGRGADESYCILVSQVKLTKESRKRLETMVSTNNGFKIAE
                     VDLHLRGPGDIAGTMQSGMPFDLKMANLATDGQLIQHARQVASEILDHDPLLENPRNS
                     IMKEQLSRLEFHRNDYSGIS"
     gene            17206..18027
                     /locus_tag="PLW22_06625"
     CDS             17206..18027
                     /locus_tag="PLW22_06625"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOU74416.1"
                     /translation="MKLYWLILLFLTPIITLKGIAQQPHACRITNTAFVAGERVCYEV
                     SYTWFFIWTDVGEACFTVDSDNRFGKTLLHLKATGKSYPFYDWFFKVRDLYESWVDPV
                     SLSPHYYNRNIYEGGFTKENEYWFNQPGDSVKIRVRRKGGPNKFVSVKSPGCLFDVVT
                     AIYYSRCLNFSGIRQGTVFPVNVLMDEEIFNVKYRYLGKDVRKVTGIGKMACLKFQVD
                     LVAGDIFSTNQKLLVWVTDDANKLPVYIESPIRVGSIQARLKSYQGLRHKLVSKN"
BASE COUNT         5251 a         4135 c         3795 g         4957 t
ORIGIN      
        1 ttctttggtt ctttcttgta tcaagacaag aaagaacatg taaaccgctc tttgcggtgc
       61 ttttatgttg cgatgcaatg aattgaaatg tggctatgga aaaaattttg aggggattag
      121 ccaatagaag cacataaaat actcttcact tctgacttat cgaggcaatc ccgattaaac
      181 gataaagtta aaaaaagaaa gaagctaccc cttgggtagc ttcttaactt ttactaagtc
      241 tactagaact tatactcgtt gtcttcaata agtttatcgg caatgcgacg gcgggcatcc
      301 tttacattta ccgatggagc cttacaaagc ctgtcaattg cagcagttaa cttactaagt
      361 tcgggctcag cagcaaagga gtttgctgca tccatggcat tcttacgaat gcggccagca
      421 gcatcgtaaa caaacacatc aacaatatcc ttatagatat tgatattttg atgaccctta
      481 ctttccaact tcttaaccct tagggcaagc gactcggcca catacagatc catgatgata
      541 ttgctcaggt tgttctgaac ctcctgctca gttgcaaact gcttaccaaa agctttgttt
      601 gctccataaa tacccagcat ggcaaccttt ttgaagttcc ttatggtctg cattttttgg
      661 tcatagtaat cgctcgaacc cgcttcagcc acaatgctat cgaggctatt gtatagctgc
      721 tcagcaggcc ccatcaaatc gtaatcgccc ttgagggagc gcttaatggc ggtatcaatt
      781 accagcaagc ggttgatctc attggtaccc tcaaagattc ggttaatacg cgaatcgcgg
      841 tagccgcgct caacgttcat ctcagctgag tagcccattc cgccgtgaat ctgaacaccc
      901 tcatcaacca caaagtcgag catctcggaa ccgtttacct tcagaatggc atcctcaacg
      961 gcgtagtggc taatggcatc aatggcagca cggccatact cacaaccctc agccttatac
     1021 ttttcaatca ggtcatcaat atcttggctt acacggtaaa tggccgattc gtgagcatag
     1081 gctctaatta ccatttcagc taacttatgc ttgatagccc caaagtttgc aattttggta
     1141 ttgaactgct tacgttcatt ggcgtactga accgactggg ttatggtttc tttagcagca
     1201 ccaatcacat tggcacccaa cttcatacgt cccatgtgca aaatgctaag ggcaatacgg
     1261 aaaccttcgc cgcggttacc tagcatattt tcaacaggta cctttacatc gttgtagtag
     1321 atttgagcgg ttgatgagcc cttgataccc attttatgct catcggggcc aattacaacg
     1381 cctggccact tgctttcgac tataaatgca ctcagcactc ggtcgttatc caccttggca
     1441 aaaacaacct gtgtatcggc aaagccggca ttggtaatcc acattttttg accattcagg
     1501 atgtagtact taccatcttc cgatagcttt gctgtggttt ttccggagtt ggcatcgcta
     1561 cctgcaccgg gctctgttag gcagtatgaa ccaatgagtt caccagttgc aagacgcgat
     1621 acatattttt ggcgctgctc ctcgttgccg tagtaaagaa tgggcattgt accaattccg
     1681 caatggcaca tgtaggccac ggaaaacgaa tatccagccc ctgtagtttc ggcaacaagc
     1741 atttgggtta caaagttttg cccaaaacca ttatactgct caggcactgc aatgcccagc
     1801 ataccaagct cgcccgattt ttgcaaaatg ctcctcatca gctcacggtc gcccttgtcg
     1861 atatcgttca ggcgagggaa aacttcagcc tccatgaaat cgcgacaggt ttgggctatc
     1921 atccgctgtt cctcattgaa ttcctcggga ataaaaatat cgttagcgtc aatctcattt
     1981 acaagaaatt cgccactttt tagcaatttt ctttcgctca tagctgtttt tggtttattg
     2041 attagttata gatttagcga ttctgcaata agttcagcaa tatcgaggtt tcgcatcttc
     2101 tcttcctgat tcttatactt gataccatcg gtcatcatgg tcatgcagaa ggggcaagcc
     2161 gttgcaatga tgttggggtc aacctttaaa acatcatcca tacgctccag gaaaacctcc
     2221 ttgttaccgt gttcggcctc cttaaacatt tgagcaccac cagcgccaca gcacagggca
     2281 aaactcctgt tgcgctccat ctctactagt tcgccaccca actttttaag tatggcacgt
     2341 ggctgctcgt aaataccatt ggcacgtcct aaataacaag gatcgtggta tgtgattttg
     2401 gcactcttga atttatcggt atcgatactc aatttgccct cgtcaatata cttattcaga
     2461 atatcgatat agctgataac ctcaaagtcg gcacccaaat cgggatactc attcttaaag
     2521 gtattatagc agtgggggca aatggttatc accttcttta cttcgtatgc cttaaaaagt
     2581 tcaatattct gtaaggcctg catctgaaat agcatctcgt ttccagccct acgggcagga
     2641 tcgcccgtgc aggtctcctc cttgcccaat acggcaaagc taacgtttag gtgaaccagt
     2701 attttggcaa aagcacgagc cactttctta tagcggtcat cgtaagcgcc ggcacagcca
     2761 acccaaaaaa ggtattcggg cttttcgcct cgggcaaaga ggtcggccat aaccgggatt
     2821 tctattttgc gagtttccat tttccaatta gtttaagttg atactgatag cagggaacgc
     2881 aatgctactg tacgttaatc tcaagatctt ttgcccaaac caagcgatcc tcaggcgaat
     2941 actgccaggg agcaccattg ttctcaatat tgttgaacat ggccttgagc tcaccgggtg
     3001 ccgacccttc ctccatcacc aggtacctgc gcatatcaac aatgagtttg ggatgatcaa
     3061 tgtttatggg gcactcctgt gcgcatgcat tgcatgtggt acatgcccat agctcctcct
     3121 cgctaatgta atcgcgtaca agcgatttcc catcagtgta atcgcgccca ttttttagca
     3181 tcataggacc tttctccttc attcgggcac gcaggtccat catcaccttg cggggcgata
     3241 gtttctttcc tgtaatgtta gcagggcata ccgatgtaca gcgcccacac tcggtgcacg
     3301 ataaggcatc aaaatagctt ttccatacta catcctcagc atctttcaca ccaaatcgct
     3361 caattggagc atcggttgca ggtgctgcaa atgcagcatt gggatccatc atcaacttta
     3421 cctcacgggt tacattatcc atgttgggca atttgccaag cggctcaagc cgcgagagga
     3481 atacgttggg tacagacatg aatacatgga aatgcttgga ataaggcaat aggtttgcaa
     3541 acaggaagat taacagaata tgtgtccacc agtaaatctg gtaatgggtg aatacagtat
     3601 caactgacat cccattgaat aaccaggtga gatgttggct aacggggtaa accccatgca
     3661 cttcatgccc tgccagctca acccacttaa ggtatgcagc attcatcccc attagcgaga
     3721 gcatgagaac caggataatt gaaagagcca cattcgcatc aatatgcgaa atcttcttca
     3781 tttcaatgcc ctcaaaacgc ttgatatgaa gaaataccct tcggataagg aataccacta
     3841 ttgaaatggc aaccagaagg gcaaaaatat cgccggtggc catcatgaaa ttgtagaatg
     3901 cgccaagaac acttagcgac ttttcaacgc caaataaccc gtcgattacc atttcgatac
     3961 tgccaaagag gatcacacaa aagccccaga acacaagggc atgcattaat ccaataactg
     4021 gcctgcgaaa aattttggtt tgcccaaaag ctacctcaag ggtaacccat agccttttac
     4081 ccagcttact taccgcaaat cccttacgtg taagcatgaa aaaccttaca attcgggata
     4141 tggtgtaagc aaaaacaccg atggtaacca gcagggctat ggcaaatact atttgtttgg
     4201 tcatgttttt ggtttttggt ttacaatggt ttgttggtaa aaacaaacat ctggagtaag
     4261 tgcctctatt ttgcctaaga acctgctgca aacgcaacag gttcatgtct tatgatttgt
     4321 aagaatggtg aactactttc cttttatctc tttaacagcc tcaaccagct ttggtaaaac
     4381 cttttgggca tcgcctacaa taccgtactg ggctacctca aaaattggtg ctgatttatc
     4441 gttgttaatg gcaactatgt acttggaacc gctaacacct gcaatatgct gtgttgctcc
     4501 tgaaatacca actgcaatgt aaaggttagg agcaataatt ttaccggttt gtccggtgtg
     4561 ctcatcgtgt gggcgccatc cttcatcgga aactgggcgc gaacatgctg tggctccgcc
     4621 aagcaattcg gcaagctcaa ctattggccc ccagttatcg ggcgatttca ttccgcggcc
     4681 acccgataca acaatttcgg catcggttaa aagcagctta ccggtttgct tctgaacatc
     4741 cttaaccaca gtttttggtg cgtcaacctg aatatcaacc ctctcaatct ttacatcttt
     4801 gctgttctca acaatatcga aagagttttg tgtaagagca ataattttga tatcggtttt
     4861 aagcactacc tgtgcaaaag caccacctga aaaagcgcgt ttgtaaacgg taaatggcga
     4921 taggcttagg ggaagttgcg atactcccga acccaatgca gctttaagcc ttaccgataa
     4981 acggggtgca atggccttac cgctgttatt gttagcaatt acaatcacct tgctaccttc
     5041 cgactgagca acctgagcaa tagcgctagt gtatagctgg ctatcgaggt ttttcaggtt
     5101 atcgctcttt aggcaaataa ccttatcggc cccatactta ccaagttgtt tgagctcatc
     5161 gttggacacc tcaccaatgg taactgcagt ggtggtagtc cccatcattt cggctaactt
     5221 gccggcgtac gaaacaagtt cgaatgataa ttttttgaat ttaccgtccc aattctcggt
     5281 atatactagt actgacatgt tttacaaatt ttttggttaa accttaaatg agtttggtct
     5341 cgttttggag tagcgtaata agttctttgg gattttcggg atccacaaac ttacatgccg
     5401 ccctgggttt gggcatttca aacttaacat actccgttag cgcatcaact gcaacaggtt
     5461 catacagttt gatgggcttt gtacgagcca tcattatacc gcgcatggca gcaatacggg
     5521 gttccttggc aatacccttc tgcactattg caacaaaagg agttggcacg gtaagaactt
     5581 ccttaccacc atctatttca cgggttatga ttgcttgacc attctcaacc tttatattcg
     5641 atacagctga aaccgatgga ataccgagga attcagcaag catgctacca accgcagagt
     5701 tgttatagtc gctcgattca atcccgcaca taacaatatc gaattgttcc tttttaacca
     5761 cctcagccag ttgggccgca gcatagtatg catcggtagg ttcagcattt acacggatgg
     5821 catcgtcggc accaattgcc aaagccttac gaatggtagg ctcagtagtg gcattgccaa
     5881 catgtgcaac ggtaacgctt tttacgccag tactggcatc atcctttaac tccaaagccc
     5941 gggttaacga tagttcatcc caggggttaa tcacccactg tatcccatta gtgtcgagct
     6001 tagtatcgcc atctacaaac ttcaccttgg ttgtggtatc gggtacgtgg ctaatacaaa
     6061 caagaatttt cattggtact gattatttat aagttaaaca tctatttagc aatcctttta
     6121 attatttaaa cccacaaatt taaactaatc ttggcataaa caagcctctt atttttaaaa
     6181 acagccatat ataattgtcc taatgccagt tacaaaatat tttatttttt taacagttac
     6241 acattcaaac gagcgtacaa atctatatca attccgttta caaccaataa ggtttttgct
     6301 tttaacaatc gattgtcaca ccatttatgc ttatgatttt gactatcaac attttgcaaa
     6361 ttgataaatt ttaacagttg tttagtcatt ttttaactaa agttgaccaa aatcacccta
     6421 acaaaattga cttttaaacg tttatgaaat aaatttacat taaacggatt ggttaatgag
     6481 caacgaggta atacatagct tgattcgaaa tcttgacgag aagctttcat actcgctggt
     6541 gcagcttgag aaggtttcgc aggaagttac tgttcacctg caaaacaacc ccgagcattc
     6601 atcggacgag cttgttccgg cgctaattcg ccattttgac attaggcgcc aaagcatttc
     6661 gtacctcaag ctaatgctca agttgcgttc cttcctaaat aacgatagta agtacatctt
     6721 aaactttgag aaaagttctg gcataagaat tgattgtata tcggagcaaa tgataattaa
     6781 cgagaagatg cctacttacc ttagcaataa cttgaacgtg gatgccttaa acatggaggt
     6841 ttacacatca aaggcttacc agaggtattt tgaggaaacc tacaacgagc ttatgcgtgc
     6901 cgataggttt gaggcatgct ccatacttga aacaatgaaa ttttcatata attaggtttt
     6961 ggtttaggtt tacaataggt taggtggttg acatccgggg cgcttaaaac gttccggatg
     7021 tcttcttttt taacctaagt gacatgaaaa gttaaaagtg aaaagtgaaa agaagaattg
     7081 agaatataag ggggttagag agagtttatg ttcgttaaac gtgagccgtg agccgtgaaa
     7141 cgtgaaacgt gaaacggaag aattctgagg aagttagagg gagttagagg aagttagagg
     7201 aatatgaaac attgagctcc accaaacact aaccactaac cactaaacac taaactggtt
     7261 tgacccccta taaaactgga ctaaaagtac aaactaaaat tgtactttta tgctgatggt
     7321 aaaacaaatt cacaaacgga caccccagga aaaggagagg attctcctgg atatccagca
     7381 cctaggggtt acggcaggct gtcgtaagca tggcatatcg agggtgctat actacgactg
     7441 gctaaaccgg taccaggcct cagggcttga gggcctggag gatcggcggg gcaagagcgt
     7501 cgatgcccgc ataaagcggc ttgagaagga gaacaggctg ctgaaggagc tgctggcaga
     7561 gaaggagctc gagtccaagc tgaaggatga gctcctaaaa aaaaagtttg gacagcagac
     7621 cagaaacggg aggtgatagg caggtatgtt gcccagggaa tcagggtggc caatgcggta
     7681 aggatggcag gcatgaagag aagcacgtac tactaccggg gaaagggcag ccgaaagggt
     7741 aagcgcccct cgacccacac cgtgaaaggc ggcgtgatgg ttccaaacga ggccgttgtg
     7801 caggagatag ccaggctcgt tgagcctgag taccatgact atgggtacca gctggtaacg
     7861 gagcttctca ggcagatggg ctatacgatc aacaagaaaa aggtctaccg gctaatggag
     7921 gagggcaacc tgcttaaccc acccgtgagg cggggggctc cgcggagccg agcgtttata
     7981 aaatacgccg ttcccccgct ggagggccct ttcaggacgg tggaggccga catcaagtac
     8041 gtgcatatcc acgaggagaa ccggaatgcc tacctggtta ccttcctgtg cacgttctgc
     8101 cgctatgccc ctgtttggga cctgcagtac tcgatgaaaa gcaaggatat cgccgagctg
     8161 gttcttgacc tggtaaacga ccctgaggtt aaacggttca tggggaacca gcgggtaaag
     8221 atagtgatca ggaccgacaa tggcccacag tttatagcca gagccctggc cgaaatcctg
     8281 gaatccctcg gtattgccca tgagtttacc atgcccggaa cgcctcagca gaatgcccac
     8341 attgagtcct ttcacagcac ggttaccagg ctggtgtgca acaggaacgt attccgcgat
     8401 atagatcacg ccagggagat cttcagggcg ttctactacg cttacaacga gacaagggct
     8461 atgaaatcgc tccttagcta ccctcccaga aagttcctaa ggctctggga ggccggaatc
     8521 gtgggcatca ggagagataa aagaaataaa gatttttttt tcttcagaga gaagcctttc
     8581 ccccctacag ggattggctc ctcccctgaa gacttgattt gggcaaacaa aaatagtatt
     8641 tttgattctt cagttctaaa ccctttggaa attagtccag tttaaagggg gtcaatacaa
     8701 aacactaaac actaaacacc aaacactaaa cactatccac taaacactaa acaccaaaca
     8761 ccaaacacta tccactaaac accaaacacc aaacactatc cactaaacac nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaacaccaaa cactatccac taaacaccaa
     8941 acaccaaaca ctatccacta aacaccaaac actatccact aaacaccaaa caccaaacac
     9001 tatccactaa acaccaaaca ctatccacta aacaccaaac accaaacact atccactaaa
     9061 cactaaacac caaacactat ttttcgtatc tttgtttcta tgggaaagtt cgaggatata
     9121 ctcaagcagt actggggcta cacatcgttt cgttccattc agctcgatat cattgaatcg
     9181 gtttacaatg ggaacgacac cctggccctg atgccaaccg gcggcggtaa atccatcacc
     9241 tttcaggtgc ccgcccttgc tatggaaggc atatgcttgg ttatttcacc cctcatagcc
     9301 ttgatgaaag atcaggttga aaacctgaaa aaaaggggca taagcgctgt agcaatttat
     9361 tcaggcatga cccagcacga gattgacatt accctcgaca attgcattta tggcgaagtg
     9421 aagttcctat acgtttcgcc cgagcgatta tccactgcca tattccgcga acgaatccgc
     9481 aaaatgcggg tttgcatggt agctatcgat gaggcgcact gcatttccca gtggggctac
     9541 gatttccgtc catcgtacct gaaaattgct gaacttcgtt ccattttaac cgaggcccca
     9601 tttttggcag ttacagccac tgctacacct gaggttgtaa acgatatcca ggagaagcta
     9661 catttccgta acggtaaggt tttccgcatg agctttgctc gcgataacct ggtttacctg
     9721 gtgcgtaacg tggaggataa agaaaagtac ctgttcaaaa tagttgataa catacccgga
     9781 agcggtatag tttacgtacg gaacagaaac aaaaccaagg aaattgcttt aatgctccag
     9841 cgcgcagggt atgctgccga tttttaccac gcaggcctta ccatggagat gcgcaacgcc
     9901 aaacagaacg actggcaaac cgggaaaacc cgcataattg tagccaccaa tgcttttgga
     9961 atgggaattg acaaacccga tgtgcgcttt gtggttcaca ttgacctgcc cgattcccca
    10021 gaagcctact ttcaggaagc cggacgtgcc gggagagacc aaaaactctc ttacgcggtt
    10081 ttgctttaca atgagtccga ttcgctaagg attgagcaac ggctcgaggc aaactttccc
    10141 ccacccgatg aaattaaaag gatttaccag gccctgggct cgtaccttaa catagcttac
    10201 ggagccggga aaggcgaggt ttacgatttt aaccttatcg atttctgctc cacctacaag
    10261 cttaacatgg tggttgcata caactcccta aaaatacttg aacaggaagg atacattgag
    10321 ctcaccgatg agctcgacaa cccaagccgc atcatgttcc tggtgaacaa acaggagcta
    10381 tacaggtatc aggttgccca tgccgaaatg gataagttta ttaaggtgct gctaaggctg
    10441 ctgccaggcg ttttttcaca gtacgtaagg attgatgagc aattcctttc caaaactacc
    10501 ggactgccag ccgataaggt atatgagaac ctgaaaacct tatcgcgcca gaaaatcctt
    10561 aactacatcc ccaaaaaacg cacacccttg gtaatattta tcgaggagag gctcgacgat
    10621 aaaaacctgc gcattgaacc cgaacgctac caaacgctga gggagcgata ccgtgcccga
    10681 atgaacgcca tgcttcagta cgcccaaagc caggccaagt gcaggagcca attcctaagc
    10741 gagtactttg gccaaaccga cagctaccga tgcggtaaat gcgacgtttg caccgcccgg
    10801 aatgagcttg acatgagcaa gtacgagttc gataacatac tggaaaaaat taaagccaca
    10861 ctaaccaaaa caccactacc ccttgagaat ctgattgact caaccggctc aaaccccgac
    10921 agagttgcaa gggtaatccg gttccttcag gacacaggta aaattactgt taactgccag
    10981 ggattactgg aatgggtaat acgcaagtaa tggctttttt cttcccaaaa aacctcactc
    11041 gctcggattt gtaatttatt cgcaattccc actaaaccag caaagcgatt gttaaatcca
    11101 ttgcattcac tatcaaccat caaccaacaa ccatcaactt tctggttgca cgcgcggatt
    11161 tgtaatcgaa caaagggaac tcggcgaagc caaatttaat ttgtttttct ttccacccgc
    11221 aaaaaaagaa accacccata taccctacgt tgtaaaagcc acacaatcgc tcaaattaaa
    11281 gcaatgtcaa agagtgcagt ttttcaatca caccacttaa aatttgataa ctttgcgcta
    11341 tgaatgaaag aaagatagac ataaaaaata agcttgatgg tgggttgaca ttcaaaatct
    11401 cacggttcaa ggaacagatt aagaaaacca agccccacaa acacgaagat tattacgagt
    11461 taatatttct gtcgcaaggt gaaggttttc actgcatcga gtcggagaaa tttatggttt
    11521 cttcgccaga tttttatttt cttaaacctg gacaacttca ctattggcaa tttacctcaa
    11581 tcccgaaagg atttgtgatt cttttcaagg acactgaatt taatgccgta aaagagaatg
    11641 tattgcttga tttaatcagc aaattaaaag atactacaag gcttactttt caggccgaca
    11701 aatatccgtc ttatttatta gaggagattt ttaacgagtt tcaaatcaat acacaatact
    11761 ccaaagaaat cattcacggg ttattgaaag cattgttcgg gaagttattg caaataaagg
    11821 ctgaaaaccc gaatagcccc aaccaacccc aacccattta tgagcggttc gtacaattat
    11881 tggttaaaga atgtccacgg cttcacaaag tcaatgagtt tgccgatttg cttaacgtta
    11941 caccccaaaa cctgaatatt atttgccgta aacagacagg caaaagtgca agcgaaatca
    12001 ttacaaacca actcttatta gaagcaaaaa gatacattct ccacaccgac aatacgatta
    12061 acgaaatagc cgaaatctta tcgttttccg atgcttctaa ctttgtaaaa ttcttcaaaa
    12121 aacacgaagg cattacaccc atacaattca gggataaata ttttcaataa taccatatta
    12181 acattcaaac ttaccaatcc aaagtcaatc atacatccta tttttgcact atacatttag
    12241 aaaaaagttt gtatggaaat agtaataatt tgtttggcgg catttttcac agccgtttta
    12301 acgttctttt cgggtttcgg tctaggaact atcttagctc ccgtttttgc aatattcttc
    12361 cctattgata ttgctattgc tctcactggt gtagtacatt tttcaaataa cattttcaaa
    12421 atagccctgg tcggcaaaaa ttcagacaaa actgttttgc ttcgttttgg aatacctgct
    12481 attttatctt cgtttgcagg agcttggttg ttaatgaaaa ttacagttct acccacactt
    12541 ttacaatacg aactttgggg caggactttt gaggttacac cagtaaaact tataattgct
    12601 atattgttaa ttgtattttc gttacttgaa atttcacctt cttttcaaaa ggttcaattt
    12661 ggtaaggaca aattaatatg gggtggactc cttagcggtt tctttggcgg attatcgggc
    12721 attcagggtg caatacgcag tgcgtttctc ataaaaatcg gactatcaaa agaagtttat
    12781 attgctacag gtgttgttat agcatgcttg gtggatttta caaggctttc ggtttatgct
    12841 tcacgcttta tttcatcaac cctgcacgaa aaccttacac ttttaatttc agcaaccctt
    12901 gcagcaattg caggggcata tttcggaaaa aggttactga aaaaggtaac ctttcgaagc
    12961 attcaacttg ttgttgcagt aatgctcatt ttaatatcaa ttgcccttgg tgctggatta
    13021 atttaaaaac taacgagaaa tgaaaataat taataattta ttacaaagat ggtatttacc
    13081 aatttcgttg aaatttctat cactcattgc atatataata ttaatcgtaa cagggttaat
    13141 ggcgtattca accgattccg cttttctcaa gcaattacgg aatacaaatc ttggtaattt
    13201 aattgtttgg tcgtactggt ggccagccat tgttattagt gctatatttt tggggcgtat
    13261 atggtgcatg gtttgccccg tagaattaat tacaactttc ttcgctaaaa tagggcttaa
    13321 acggaaacgc cctgcatggt tgctttcggg ttgggcgatt acagtttttt atatcatcat
    13381 tctgtttatt ggcatacaag gttttgcaat tcaccgcaac ccgtttttta tggctgtcta
    13441 tcttttggca attgttgggg tttcggtaat tattggaagt atttatgaga aaaacacatt
    13501 ttgtcggtac gtttgcccag ttggttattt gttgggatta tattcaaggc tttcattttt
    13561 cggttggcgt gttaaggata agaatatttg caatagctgt aaagataaat cgtgcatcca
    13621 caagaaatac cagtacaatt taaactataa aagttgcggg gtcgatttgt accctgccaa
    13681 catcactgat aattccgatt gtattttatg tgctggttgc cttaaaacgt gcagcaaata
    13741 tcaatcggaa cccaacgctg agcgtcctaa cccacaattc acatacattg gttttgccaa
    13801 cggccttttt caaatgaaac ctttgaaaat ggcagaattg atttttgttt tggttgtttc
    13861 ggggtttgta atttccgaaa tttggtcgga atggaaaata accgatgctt acctttcctt
    13921 tctacccgat ttggttgtaa agcctttagc aattgaaaac aaattcgggt cgggattttt
    13981 aaaaggtaca atactatttg cgttcgtgcc tttcatctta tggttacttc catttctttt
    14041 tgcccggttg gctggtgttg caatcaaatt caaagcgtat ttggtcaatt acggcattgc
    14101 ttttattccc attattgcag ccgcccattt ggcaaaagcg gttttaaaat ctacatcacg
    14161 aatcccttat tttgaatacc tctttgatga ttttacaggc gtttctacag cgcaaaagat
    14221 aattgaaggc aatattacct tacaacaaac cccaccttgg ttaaatacac tggtctcgtt
    14281 gcttttaatc ggttttatgc ttattggagt ttggttaagt attaaggttg taaagcattt
    14341 aaatgtgaag aatggtaaag aaaagtcatc aaaatcttac tatctgatac caatacttta
    14401 cggaggtgtt tttctgataa tgttattggt ttggcgatgg ttttaattga ttgttttaaa
    14461 tttttggtca cacggatttg taaccaacaa aagggagtct ggtaaaacca ttccatatca
    14521 accgtacatt actattccac agttatttac aaaataaaca gtaatccaga aactttttgg
    14581 cccaactttt gttagtaggt tgagtgtaaa caaaaaattt tatggaaacg ctcaatagtt
    14641 ctgaactacg ccaacgcctg tacgactact caaaccaggt tggtttcgat acccaaaagg
    14701 attcgttccg cgaggtaatc tcattcctta tcgatattga ccaaaacttt ttatacacac
    14761 tactaaaccc cgaggaggtt aggtatttgg ccacccaccg cgatgttgag gagaggctta
    14821 aacggcagct ggagcaagtg gtagaatcgc tatagtaatc caagtcaaaa tagtattaag
    14881 gggaaagttt tccccttttt tattttttgc ttactttgtg gtaagcattt caggtatggg
    14941 aaacattctc gactccgaaa taatgtactt gccgggtgtt ggccccaagc gtgccgagct
    15001 gctccaaaag gagctgaaca tatacacctt tggcgattta atcacctttt acccgttccg
    15061 ttacattgac cgaacaaaaa tcaacaaaat atcggaggtt accgatgagt taccctatgt
    15121 acaggttgtt gggcgagtta tcagcatctc agaggcgggc gtaggtcggc accgtaggct
    15181 aacagccatt gtaaccgacg gcacaggccg aatggaactt gtttggttcc aaggcataac
    15241 gtgggttctt aaacggctaa aacctaacgt cgactatctg atatttggca aggcaacaag
    15301 gtttggcgcc cactttagca ttgcccatcc cgagatggaa atttataatc ctcagcagga
    15361 tgttattacc gggtcgctac agccggttta ctccacaacg gaaaagctta aaaacaacca
    15421 catcacatcc aaaaccataa tgggatttgt tcaaacccta atcccaatgg cgattggaaa
    15481 aatcaccgaa accctgccgc ccttcatcat tgagcaaaac aagctaatga ccctacctga
    15541 agccttacgc aacattcatt tcccggcaaa caccacactg ctccacaagg cgcagcaccg
    15601 gcttaagttt gaggaacact tttacattca gctcagtatc cttagccgtc aaaaggttcg
    15661 ccaaaccagg gttaacggtc atgttttcag ccatgtggga gagatgttta accggttcta
    15721 ccatgaacgg ctcccgtttc cgctaaccga ggcccagaaa cgggtaataa aggagattcg
    15781 ccgcgatatg ctaagcggta agcagatgaa taggttgctg caaggcgatg ttggtagcgg
    15841 caaaaccgtg gttgcgctga tgtgtatgct gcttgctgtt gataacggat accaagcctg
    15901 cattatggct cccactgaga tacttgccaa ccagcactat gaatcaatca ctgcacttat
    15961 gggcgattta ccggtaaagg tgggtttgct aaccggcagt accagacgtt cagaacgcaa
    16021 caacctgcat cagtcgcttg aggcgggtga actcaatatc cttattggca cccatgcgct
    16081 actggaggat gtggttaaat tcaagaacct tggttttgtg gtaattgatg agcagcaacg
    16141 ctttggcgtt atgcagcgcg ctaagctctg ggaaaagaac aacgacccac cccatgtgct
    16201 ggtaatgaca gccaccccaa ttccccgcac gcttgccatg accctttacg gcgatttgga
    16261 ggtgagcgta attgatgagt tacctccggg tcgtaaaccc gttaaaacat tacactacac
    16321 cgatgccaaa cgtaaccttg tttttgcgct tatgaaggag cagattaaaa ggggccgaca
    16381 ggtttacgtg gtttatccgt taattaagga atcggagaaa cttgagaatt tgaaagatct
    16441 tgaggatggg tacgagagca ttgttcgggc atttccaccg ccagagtatg taaccgtggt
    16501 agttcacgga caaatgaagc ccgaagacaa ggactactcc atgaacctct ttgcgcaggg
    16561 caaagcccac ataatggtgg caaccaccgt gattgaggtg ggagttaatg ttcccaatgc
    16621 cacggtaatg gtaattgaga gcgctgaacg atttgggctg tcgcagctgc accagctccg
    16681 cggacgggtt ggccggggag ccgatgaaag ctactgcata cttgtttcgc aggttaagct
    16741 aaccaaggaa tcgcgcaaaa gactggaaac catggtttcc acaaacaatg ggtttaagat
    16801 tgccgaagtt gaccttcacc ttcgtggccc cggcgatatt gccggaacta tgcaaagcgg
    16861 aatgcctttc gacctcaaaa tggccaacct tgccaccgac ggacaactca ttcaacacgc
    16921 ccgacaggta gccagcgaga ttcttgatca cgacccctta ctggagaacc cgcgaaactc
    16981 catcatgaag gaacagctca gcaggctaga gtttcatcgg aacgattaca gcggaatatc
    17041 atgaaatacc catgtaaggc agcttgcaca aacacagatg aacaaccgag cgtttatttg
    17101 cgagctcgac gaagtcaaac tatgggtgtt ccatccgctc gataaagttt attaaaagca
    17161 agactgtaac gtgttattaa ttatcaatat aaatatttcg tacggatgaa actttactgg
    17221 ttaatccttc tttttttaac accaattatt acccttaaag gcattgcaca gcaaccgcat
    17281 gcctgcagaa taaccaacac ggcctttgtg gctggcgaaa gggtttgtta cgaggtaagc
    17341 tacacctggt tttttatttg gaccgatgtt ggcgaggcct gttttactgt ggattccgac
    17401 aaccgatttg gtaaaacatt gctacacctt aaagcaacag gtaaaagtta cccattctac
    17461 gattggttct tcaaggttcg agacctttac gaatcgtggg ttgaccctgt gagtttatct
    17521 ccacactact acaaccgcaa tatctacgag ggcggattta ctaaggaaaa cgagtactgg
    17581 ttcaaccaac ccggcgatag cgtaaaaatt cgggtacgcc gcaagggcgg gcctaacaaa
    17641 tttgtttcgg ttaaatcgcc cggttgcctt tttgatgtgg ttaccgctat ttactactcc
    17701 cgatgtttaa atttttccgg cattaggcag ggtactgttt tcccggtaaa cgtattgatg
    17761 gacgaggaga tttttaacgt caagtacagg tacttaggca aagatgtgag gaaagtaaca
    17821 gggataggga aaatggcctg cctgaaattt caggtcgatt tggtggcagg cgatatcttc
    17881 tcaacaaacc aaaaattgct ggtttgggtc accgacgatg caaataagct accagtttac
    17941 attgagtcgc ccatacgtgt tgggagcata caggcacgac taaaatctta ccaaggacta
    18001 cgacacaaac tggtaagtaa gaattagctg aaaccttgca ttaaagaatc gttttaactg
    18061 atatttcatt tcactactgt acgattaaaa tcttaatcct accctgacag tcccgtaggg
    18121 gctaaatatt tttaactcca tgggactccc gtatgtatag cgatgctcgc tatgatactc
    18181 caaagagcaa cgctgaatag ttgcatcgca acaggagaac attccaaaga gcaaaaag
//
LOCUS       DAOOWJ010000052        18233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3072_length_18233_cov_7.571680, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000052 DAOOWJ010000000
VERSION     DAOOWJ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 18233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18233
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3072_length_18233_cov_7.571680"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1341
                     /gene="hydF"
                     /locus_tag="PLW22_06630"
     CDS             112..1341
                     /gene="hydF"
                     /locus_tag="PLW22_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOU74417.1"
                     /translation="MQKGKETKPHIGIYGRRNNGKSSLINLLAGQNVAIVSDFAGTTT
                     DPVKKSFEITGFGPVILVDTAGIDDEGELGQKRVEKTLQSINHIDLALLVVTSNTWGD
                     YEEMLVNEFIKTDTPYLVVHNKSDIEPASSIFKEKLSGKRHSGFIDISCKSQTQPDEL
                     ISLIKKAIPESSYRTPTLLGDLISYGDIVLLITPIDVEAPAGRLILPQVQAIRDVLDN
                     DAVAVVLKEREVDAFLRKTQVKPALAITDSQIFVKADASIPRDIPLTSFSILLARLKG
                     DFENYLKGTPKISELKDGDRVLLLESCNHHVSCDDIGRVKIPRWISNFTGKQLEYDVV
                     AGLDALPRDIMEYSLVIQCGGCMITRKQLINRLRPAVEAGIPVTNYGMAIAYVQGIYQ
                     RAIAPFTANKESKADYL"
     gene            complement(1345..3810)
                     /gene="priA"
                     /locus_tag="PLW22_06635"
     CDS             complement(1345..3810)
                     /gene="priA"
                     /locus_tag="PLW22_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOU74418.1"
                     /translation="MTSQLYADVILPLALQATYTYSVPFEMEKMIQPGVRVVVSFGQR
                     RLYSALVYRVHSEKPQVQQVKDILQVIDTTPIVLPSQYELWEWLAEYYMCSLGEVMKA
                     ALPSGLKMESDTYIASGDFDEIDLSDEEMTLIHLLGQKRKLTIQQLTQKSGLKHPMSV
                     IKSMLERKLLFITEDIESDYRPKTENYIALAPEYSDEKKLCELIDSLKRAPAQQRLIL
                     AIASQATTPNDLSTARVLRKTLLEKVDVSASVLKSLIDKGYIVQDEVETSRIDNANSD
                     PATRNPLALEDFQINALNEIQNQFSSHDVVLLHGVTSSGKTELYIHLINQALQNGKQV
                     LYLLPEIALTTQIINRLRKVFGNRVGVYHSKFSDSQRVEVYLKMLSEEPFDVILGVRS
                     SIFLPFSNLGLVIVDEEHENTFKQYNPAPRYHARDTAIVLAKQVGAKVLLGTATPAVE
                     TYFNAKSGKFGLVKMLERFSGLELPRTIVVDTRDARKRKRMKSHFSDTLLSAIENALS
                     QKEQVILFQNRRGYAPFIECADCGWVPHCEHCDVTLTYHKYTNQLTCHYCGFGMEQMG
                     SCLACGSTNLQPKGFGTEKVEDELSVFFPDAVIERIDLDSTRSRSSYERIISGFEQGK
                     IDILVGTQMVSKGLDFDRVSLVGILNADNMLNFPDFRAFERSFQLITQVSGRAGRKGK
                     QGLVIIQTAQPDHPVIKSVTGNNFFTFWEWQLAERNRFHYPPFYRLVRITLKHKDKHH
                     LEDTAENLAKTLRHSLGENVLGPEPPIISRVQGLYILEILVKIKREQPLQRIKEFIRY
                     SITMLKQKRETSTVVIAIDVDPY"
     gene            4584..5255
                     /locus_tag="PLW22_06640"
     CDS             4584..5255
                     /locus_tag="PLW22_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOU74419.1"
                     /translation="MIEMFNSSIGKKLVMSITGLFLMVFLLVHLTVNMLLLVGDGEVF
                     NRAAHFMASNPAIKVIEPLLAIGFVFHIIYATFLTIKNQRTRPIGYSKTTSNGLSTWS
                     SKNMFILGTTIFIFLIIHLANFFWKLKFGTVETITYGSDTMPNAYALVSGLFISYWWY
                     DLLYVIGAIFLGLHLSHGFWAAFQTLGWNGTKWLKRLEVVAYVYAIIIGTGFSIIPIY
                     FLLFR"
     gene            5286..7205
                     /locus_tag="PLW22_06645"
     CDS             5286..7205
                     /locus_tag="PLW22_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOU74420.1"
                     /translation="MSKLNAKIPEGPLAEKWTNHKAKLRLVNPANRRKLDIIVVGTGL
                     AGSSAASTLGELGYNVKVFCYQDSPRRAHSVAAQGGINAAKNYKNDNDSVYRLFYDMI
                     KGGDFRAREANVYRAAEVSNLVIDHYTALGVPFARDYGGLIENRSFGGVQVSRTFYAK
                     GQTGQQCLIGAYSALSRQIDKGTVTMYPRREMMDLVVIDGKARGIIVRNLVTGEIERH
                     SAHAVVLATGGYGTIYYLSTLALNSNGSAAWRAHKRGALFANPSFVQIHPTSIPVLND
                     HQCKLTLMSESLRNDGRVWVPKTKGDPRKPNEIPEDERDYYLERRYPAFGNLVPRDVA
                     SRAAKERCDAGYGVGPTGLSVYLDFRDAIQKKGRKYIEENYGNLFEMYEKITGESPYE
                     VPMRIYPAGHYTMGGLWVDYNLMTTIPGLYAIGEANFSDHGANRLGASSLMQTSGDGY
                     FILPYTIADYLADEIKTPKIPTNTPEFDAAEKEVKERIERLMAINGTETATSFHKRLG
                     KIMWDYCGMVRNEEGLKKALTLLDELKDEFWKNLKVPDTASELNQELEKANRVADFLE
                     LGKLMVYDALNRKESCGAHFREESQTETGEAKRDDENYSYVAAWEFTGDGKEPILHKE
                     ELKFEYVKLQERSYK"
     gene            7224..7979
                     /locus_tag="PLW22_06650"
     CDS             7224..7979
                     /locus_tag="PLW22_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOU74421.1"
                     /translation="MLKAMKVKLKIWRQKDSRSKGKFEVYELDNVAPDMSFLEMLDSL
                     NQRLVREGKSPVAFDHDCREGICGSCGIYINGRPHGNDHRTTTCELRMRRFKDGDTIT
                     IEPWRAAAFPVVKDLVVDRSAFDKIIQAGGYISVNAGAAQDANAIPVPKHNADVAFDT
                     ASCIGCGACVAACKNASAMLFVAAKITHLAQFPQGRVEAPERVKRMVAKMDELGFGSC
                     TNTRACEAECPKGISISTIAKLNREYITAKLKD"
     gene            7981..8877
                     /locus_tag="PLW22_06655"
     CDS             7981..8877
                     /locus_tag="PLW22_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU74422.1"
                     /translation="MPTKKDKSPDILLVPVDFGDYYEKAIEFAQKLAPITESQIHLLH
                     VLDLKDWWLDEVKEERLINEAFNKLLNISRQFGLPEDSKLMVQTGKRHHKIVEYAEQE
                     NVRFILMVDNYPFAQEGKRIGSTLENVIMKATKPVITVKTVPETIFKNMLVPLDLTKN
                     CRVKLFNSVALALKFDSKLNIVSVIFGDIDPEKSRINEKIEKYAAVYAENHIPHTKKL
                     IRKDEDFAYQEILDYADEIKCDSIIIMTHKENVGFDNYVGAFAHHIINEAKVPVISLN
                     NASSTQTTRDLLSSIIDPLNIF"
     gene            8931..9003
                     /locus_tag="PLW22_06660"
     tRNA            8931..9003
                     /locus_tag="PLW22_06660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8964..8966,aa:Gly,seq:ccc)
     gene            9151..9531
                     /gene="rsfS"
                     /locus_tag="PLW22_06665"
     CDS             9151..9531
                     /gene="rsfS"
                     /locus_tag="PLW22_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOU74423.1"
                     /translation="MAVKRVKKTSNTEDLLTCIVDAIQEKKGKNVVSLEIGALPNAVC
                     KYFVFCNADSTTQVDAIANNIEDKVLEKLGEKVYRSDGYQNAIWIVLDYIDIVVHVFQ
                     TEWRNYYRVEALWADAKAIQYEEV"
     gene            9563..11584
                     /gene="ftsH"
                     /locus_tag="PLW22_06670"
     CDS             9563..11584
                     /gene="ftsH"
                     /locus_tag="PLW22_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU74424.1"
                     /translation="MAENNNNNPMMNDENGKPKMPRFNVYWVYIIIILGFFLIQYFYS
                     GKTAQLTTWQEVKNQMLEKQEISKLLVVRNAGKVEVYLKEDKLEKYKDRLGQGFGAVP
                     KAGPHFYFTIGSIETFERQLAEAQTNIEESQKIYPEYVDRPNYWADILSWVLPFVILA
                     AIWIYIFRRISRGGGSGPGGGNIFSVGKSKAQLFDKETRPRVDFKDVAGLEEAKIEVM
                     EIVDFLKNPKKYTELGGKIPKGALLVGPPGTGKTLLAKAVAGEANVPFFSMSGSEFVE
                     MFVGVGASRVRDLFRQAKEKAPCIVFIDEIDAIGRARGKNPSFSGNDERENTLNQLLT
                     EMDGFASNQGVIILAATNRADILDRALLRAGRFDRQIHVELPDLKERLDIFKVHLRPL
                     KLEENLDISFLAKQTPGFSGADIANVCNEAALIAARKNKKYVEKQDFLDAIDRIIGGL
                     EKKNKIISKEERRTIAYHEAGHATVSWLLEHANPLLKVSIIPRGRSLGAAWYLPEERQ
                     ITTTEQLFDEMCSTLGGRAAEEINFNKISTGALSDLERVTKQAYAMVAYFGMGKNLRN
                     ISYYDSTGQVEFTFSKPYSEKTAEQIDEEVKEIIDKAYERAKQILTENHEGHVQLAEL
                     LLEREVIFSEDLEKIFGPRKTLSREQELVKELDEEAHKQNSESKENQTE"
     gene            11588..11791
                     /locus_tag="PLW22_06675"
     CDS             11588..11791
                     /locus_tag="PLW22_06675"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOU74425.1"
                     /translation="MDQNWQEVFSTSHTWQAEMAKQILEENEIPAVVVNRKDSSYLFG
                     EVAVYTEPENAERAKELLKDIQG"
     gene            11788..12615
                     /locus_tag="PLW22_06680"
     CDS             11788..12615
                     /locus_tag="PLW22_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HOU74426.1"
                     /translation="MSEFLKRTISGLLFVIVLVGAIYIGHITFFILFLAITTATMLEF
                     YHLGLKAKLRPQSLLGVFIGMAFFIWSYFYSSGRIEQLTLYGFVPLLVSIFVVELYRH
                     QLKPMQNIAFTLLGIFYIALPFSLMNFITINGSSYSMDYNPNILLGILFLVWANDTGA
                     YIVGVSMGKHKMIPRISPKKSWEGFVGGIATTLLVAWVIAMFFDEVSLKHWLAIGLIT
                     TFMAVLGDLVESMFKRSIGVKDSGKFLPGHGGLLDRFDALLMVLPMVYVYLEVMMII"
     gene            12630..13280
                     /locus_tag="PLW22_06685"
     CDS             12630..13280
                     /locus_tag="PLW22_06685"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOU74427.1"
                     /translation="MKVHKEGYSILFVTLAVLVVLCAVSWLILPKAISIVATVASVLL
                     MAFLTRFFRVPSRHIETRLNAVLSPADGTVVTIEEVEENEYLKTRCIQVSIFMSVWNV
                     HINWFPVSGVIKYYKYHPGDYLVAWHPKSSSHNERTTVVVERPDGVKVLLRQIAGAVA
                     RRIVCYAEVGKPVNQCSELGFIKFGSRVDVFLPLNADIKVAIGQKVTGNLTVIATV"
     gene            complement(13288..14079)
                     /locus_tag="PLW22_06690"
     CDS             complement(13288..14079)
                     /locus_tag="PLW22_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOU74428.1"
                     /translation="MFEIKLVGMTLSIAERHRKILEKLKETGYVSVVELSEQLGVSAV
                     TIRKDLKLLESRNLLFRSHGSASVNNPYIADRHVIEKEQLYVEEKQRISRYAASLVSD
                     GESIILASGSTINEFSRQIGERRNITVICASLVAARELAVRGVAEVLQLGGLVRHTAT
                     SVVGPFAEKMLEGFRCSKLFMGVDGIDPEFGLTTTNALEASLNRQMIASAEKVIVLAD
                     SSKFGRRGFSRICQLEQVDLIVTDKGVNELFVKQLEEKGVEVVLV"
     gene            14205..15770
                     /locus_tag="PLW22_06695"
     CDS             14205..15770
                     /locus_tag="PLW22_06695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOU74429.1"
                     /translation="MNREQMLSQAVSNDQGFDFVIIGGGATGIGIALEAATRGYRTLL
                     LEKHDFTKSTSSKSTKLVHGGVRYLAQGDIALVREACIERGRLLKNAPHLVKNQSFVI
                     PTFGWFDELMYTVGLTFYDLLAGSYSLGRSLRVSKKRALEYIPTIEPKKITAGVVYHD
                     GQFDDSRLAINVLQTATEHGAMVLNYMNVVKLTKNTNGIVDGVVAEDLETGRSYTFHG
                     KAIINATGVFADDVLQMDIPGQAKTISPSQGVHVVLDKSFLPGNHAMMIPKTDDGRVL
                     FAVPWHNRIVVGTTDTPIPNASLEPVALEEEIEFILNTAGRYLTKAPKRSDVLSVFAG
                     LRPLAAPTGESKKTKEISRSHKIIVTQSELFTMVGGKWTTFRRMAEDMVNRVESVKGW
                     EKSKTKTRNLKIHGYTNNVNHSDPLYVYGTDKEKILELARVEPELGETLSQSLGIIKA
                     QVVWAVRNEMARNVEDFLARRTRCLLLDARESMRIAPAVAAIMARELNRDKNWEREQV
                     ENYLAIAQNYILS"
     gene            15811..17301
                     /gene="glpK"
                     /locus_tag="PLW22_06700"
     CDS             15811..17301
                     /gene="glpK"
                     /locus_tag="PLW22_06700"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOU74430.1"
                     /translation="MEKFIMALDQGTTSSRALIFDQKGNIRSMAQKEFQQIFPKPGWV
                     EHDPNEIWSSQVAVAAEAMSKIGINGQNLHGIGITNQRETTIVWDRSTGEPIYNAIVW
                     QDRRTSSLCDELRAKGFTDTIRQKTGLVIDAYFSGTKVKWILDNVPGAREKANRGELA
                     FGTVDTWLVWKLTRGRTHVTDVSNASRTMLFNINTLTWDKELLDLLQIPESILPRVAS
                     SSEVYDHTNTTFFATKVPIAGMAGDQQAALFGQMCTSQGMVKNTYGTGCFILMNTGKK
                     PFLSKNNLVTTIAWQVNGDTYYALEGSIFIGGAVVQWLRDGLGIIKSSAEVEELARQV
                     PDNGDLYLVPSFTGMGAPHWDQYARGLMIGISRGTTRAHIARAALESIAFQTMDVLDV
                     MAKDTGINLTELRVDGGASANNLLMQFQSDVLGINVVRPKTIETTAMGAAYLAGLATG
                     YWKNIEEIANLWQLDKTFTPGMPLETLQKMKDKWADAVERAKSWHR"
     gene            17347..17547
                     /locus_tag="PLW22_06705"
     CDS             17347..17547
                     /locus_tag="PLW22_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74431.1"
                     /translation="MKKIDNKKNNKMAIGLSIGLVLGAIAGITIGVLLHDIALWLSIG
                     IGSGMAFGIAIGVLIGSRKKFE"
BASE COUNT         5284 a         3990 c         4121 g         4838 t
ORIGIN      
        1 tttatatgtt taaagtaatg caagttaacc aaatgatttg aacattgtgc tgactgtcag
       61 gttatttttt ggaaaatatt tcaatacttt tgcgaagtaa caacatgatt tatgcagaaa
      121 gggaaagaaa caaagccgca tattggtatt tatggccgca gaaacaatgg caagagctcg
      181 ttaattaacc ttcttgccgg gcaaaatgta gccatagtat cggactttgc aggtacaaca
      241 accgacccgg taaaaaagtc gtttgagatt accggatttg gacctgtaat actggttgat
      301 actgccggaa ttgacgatga gggtgagctt ggacagaaaa gggtcgaaaa aaccctacaa
      361 tccattaacc atattgacct tgccctgttg gttgttacct caaatacttg gggcgactac
      421 gaggagatgc tggtaaacga gtttatcaaa accgatacac catacctagt ggttcataac
      481 aagagcgaca ttgagcctgc ctccagtatt tttaaggaaa agctgagtgg caagaggcat
      541 tcgggattta ttgatatcag ctgcaaatcc caaacccaac ccgacgagct aattagccta
      601 atcaagaaag ccattcccga atcgtcgtat aggacaccta cgctgcttgg cgaccttata
      661 tcgtacggtg atattgtttt acttatcacc cctattgatg ttgaagctcc cgcaggcagg
      721 ttgatactcc cgcaggttca ggccatacgc gacgttctgg acaacgatgc cgtggcagtt
      781 gttcttaagg aacgtgaggt tgatgccttc ctacgtaaaa cccaggttaa acctgcatta
      841 gccataaccg acagccaaat ttttgttaaa gccgatgcat caatcccccg cgacattccg
      901 ctaaccagct ttagcatact cttagcccgc ctgaagggcg attttgaaaa ttaccttaag
      961 ggcactccga aaatatcgga gctaaaggat ggcgataggg tgcttctgct tgaaagttgc
     1021 aaccaccatg tttcatgcga cgatattggt agagttaaga ttccaaggtg gatatcgaac
     1081 tttaccggga agcagctcga gtacgatgtg gttgcaggcc tcgatgccct accccgcgat
     1141 ataatggaat actcacttgt tattcagtgt ggtggctgta tgattacccg caagcaactt
     1201 attaaccgct tgcgtccggc cgtagaggca ggcatacctg ttaccaacta cggaatggct
     1261 attgcctatg ttcagggtat ttaccagcgt gccattgcac cttttacagc aaacaaggaa
     1321 tcaaaagccg attaccttta gggtttagta tgggtcaaca tcaatggcga taaccaccgt
     1381 gctggtttcc cttttttgct ttaacatcgt aatactatac ctaataaatt ccttgattcg
     1441 ctgcaagggc tgttcacgct taatctttac aagaatctcg agtatatata gcccttgtac
     1501 tctcgaaatt attggaggct caggccccag tacattctca cccagcgaat gccttagggt
     1561 ttttgccaga ttctcagcgg tatcttccaa gtggtgctta tccttatgtt tgagggtaat
     1621 ccgaacaagg cggtaaaaag gcggatagtg gaatcggtta cgctcagcta gctgccattc
     1681 ccagaaagta aaaaagttgt tacctgtaac gcttttaata acgggatgat cgggctgcgc
     1741 tgtttgaata ataactaaac cctgcttccc ctttcgccca gccctaccgc taacctgagt
     1801 aatcagctga aaactccgct caaatgctct gaaatcgggg aaattaagca tattatcggc
     1861 attaagaatg cctacaagac taacccggtc aaaatcgagc cccttggaaa ccatttgggt
     1921 gccaaccaga atatcaattt taccttgctc aaagcccgaa ataattcgct catacgatga
     1981 gcgtgaacgg gtagaatcca aatcgatacg ctcaataacg gcatcgggga agaaaaccga
     2041 aagttcatcc tcaacctttt cggttccaaa acccttaggc tgcaggtttg tactcccgca
     2101 tgcaaggcat gaacccatct gctccatgcc aaagccgcag taatgacatg ttagctgatt
     2161 ggtgtactta tggtaggtaa gcgtaacatc gcaatgctca cagtggggca cccaaccgca
     2221 atcggcacac tcaataaatg gagcataccc tcgcctgttc tggaaaagta ttacctgctc
     2281 cttttgggaa agagcgttct caatggcact caggagagta tcggaaaagt gcgacttcat
     2341 tcgctttcgc ttacgagcat cgcgggtatc aaccacaata gtacgtggca attctaagcc
     2401 actaaagcgc tccagcatct ttactaatcc aaacttaccg gatttagcgt taaagtaagt
     2461 ctcaactgcc ggtgttgcgg taccaagcaa aacctttgcc ccaacctgtt tggctaggac
     2521 aattgcagta tcacgagcgt ggtatcgggg tgcagggttg tactgcttga aggtattttc
     2581 atgctcctca tcaacaatta ctaaccctaa atttgaaaaa ggcaaaaaaa ttgaagatcg
     2641 aaccccgaga attacatcga aaggctcctc gcttagcatt ttcaggtaaa cctcaacgcg
     2701 ctgagagtct gagaacttgg aatggtaaac accaacccta ttcccaaaaa ctttgcgcag
     2761 acgattaatt atttgtgtgg tcaatgcgat ttcgggtaac aggtaaagca cctgtttccc
     2821 attttgtaga gcttggttaa taaggtgtat gtagagctca gttttaccgc ttgaggtaac
     2881 tccgtgcagc agcacaacat cgtgtgaact aaattggttt tgtatttcgt tgagggcgtt
     2941 aatctgaaaa tcttccaggg caagggggtt acgagtagcg gggtcgctat tagcattatc
     3001 aatccttgat gtttcaacct catcctggac tatatatcct ttgtcaatta acgactttaa
     3061 aacggatgcc gacacatcaa ccttttccag cagggtcttc ctgagaaccc ttgcagtaga
     3121 taaatcgttt ggagtagttg cctgcgaggc aatagcaagt attagccttt gctgtgccgg
     3181 tgcacgttta agtgaatcaa taagctcaca gagctttttc tcatcggagt attccggtgc
     3241 caaagcaata taattttcgg ttttagggcg ataatcggac tcaatatcct cagttatgaa
     3301 aagcaatttg cgctccaaca tcgattttat aacgctcata gggtgtttga gacccgattt
     3361 ttgggtaagt tgctggatgg taagcttacg cttttgccct agcagatgga taagggtcat
     3421 ctcctcatcg gagaggtcaa tttcatcaaa gtcgccagaa gcaatgtagg tatcgctctc
     3481 catttttaat cccgatggaa gcgcagcttt cattacctcg cccagggaac acatgtagta
     3541 ttcagcaagc cattcccata gctcatactg gctgggaagc acaataggtg tagtatcaat
     3601 aacctgtaaa atatccttta cctgttgcac ttggggtttt tcagagtgca ccctgtaaac
     3661 cagagctgag taaagcctgc gttggccaaa cgatacaaca acccgtaccc cgggttgaat
     3721 cattttttcc atctcaaagg gtacagagta ggtgtatgtt gcctgaagag ctaaaggcaa
     3781 aataacatca gcataaagtt ggcttgtcat tataacaact ttttcactct ggaaagttat
     3841 ataaaatagt tgttcatgct aaggcttaag cctaactgtt aatatcttta tggagctaac
     3901 tgaaacagca aaaaatttgc tttcaacatt ttgtgctttt atcaatgaaa tggtaatgca
     3961 aatcatcaaa tatcttcgtt ttgtcaaaat aattttacta acttgcgaaa aaattattcc
     4021 ttgcgggagt ttataaacag ggtagattaa ttgaagtttt agaagcgatg aaggatttgt
     4081 ttaaacttaa aggtaaactt ttagtcctaa tagttgttag tattattata tccgtaggtt
     4141 taagttcatg ccataataat attccatgtc cggcctacgc aagtgtggta aagtaaagta
     4201 tatttgtata aaattttggc ctatgaaaat tagaagaatc gtaactggat tattgctact
     4261 tgcatttgta gcagtattag caagttcatg ctccaaaaaa acttgcccag cctacagtaa
     4321 aatttaccgt agtacagtag ttaacaacta ggctatccta tcttgtattg cccacaaagg
     4381 atttgttaat cctttgaaat attgaatatt acagggtttc tcagtaagag aaacccttta
     4441 tttttattca ttatcaaggc cttatgtgtt gtcggtacat aaaatgcgtg ttggaaaagt
     4501 tatttagaat gttaaattag ttcatatgct ttagatgaaa agcaaaattt aataacttgt
     4561 actgccaatt aaaccgataa actatgattg aaatgtttaa ctcatcgatt gggaaaaagc
     4621 tggtaatgag cataaccgga ttgtttctaa tggtattcct gctagttcat ttaactgtaa
     4681 acatgctgct actggtgggc gatggtgagg tttttaaccg agcagctcat ttcatggcca
     4741 gcaaccctgc cattaaggta attgagccct tgctggcaat cggctttgtg tttcacatta
     4801 tctatgcaac cttcctgacc attaaaaatc aaagaacaag gccaataggt tactcaaaaa
     4861 ctacatctaa tggtctttca acctggtcgt cgaaaaatat gttcattctg ggtactacca
     4921 ttttcatttt cttaatcata cacctagcaa atttcttttg gaaactaaag tttggaaccg
     4981 ttgaaaccat tacttacggg agcgacacta tgcccaacgc atacgcattg gtttcgggtc
     5041 ttttcataag ctactggtgg tatgatttgc tttacgtaat aggtgcaatt tttcttggat
     5101 tacacctgag ccatggtttt tgggctgctt tccaaaccct tggatggaat ggtacaaaat
     5161 ggttaaaacg ccttgaggtg gtagcctatg tttacgccat tattattgga acgggattta
     5221 gcattatccc aatctatttt ttactgttta ggtagatttt aatccgatta taaatatcga
     5281 cagctatgag taagctaaac gccaaaatac ctgaaggccc attggccgaa aaatggacta
     5341 atcataaagc gaaactccga ctggtaaacc cagccaaccg acgaaaactc gacattattg
     5401 tggtaggaac aggccttgca ggttcatcgg cagcatcaac acttggcgaa ctggggtaca
     5461 acgtaaaagt gttctgctac caggatagcc cccgaagggc tcactccgtt gccgcacaag
     5521 gcggaataaa cgctgccaaa aactataaga acgacaacga tagcgtttac aggctgttct
     5581 acgatatgat taagggtggc gatttcagag cccgtgaagc aaatgtttac cgtgctgctg
     5641 aggtaagtaa ccttgtaatt gaccactaca ccgcgctggg agtacctttt gcccgtgact
     5701 atggtggatt aattgagaac cgttcgtttg ggggtgttca ggtttcaaga accttttacg
     5761 caaagggaca aaccggccaa cagtgcctta ttggcgccta ctccgccctc tcgcgccaaa
     5821 ttgataaagg aactgttact atgtaccccc gcagggagat gatggatctt gtagtaattg
     5881 acggaaaagc acgagggatt atagttcgca acttagtaac cggcgagatt gagcgccact
     5941 cggctcatgc agtagtttta gccactggag gttacggcac aatctattac ctctcaacac
     6001 tcgcactaaa ctcaaatggc tcagccgcat ggcgtgccca taaacgtggc gcactgtttg
     6061 ccaatccaag ttttgtgcaa attcacccaa ccagcattcc ggtgctaaac gaccaccaat
     6121 gcaagcttac ccttatgtcg gaatcgttaa gaaacgacgg ccgggtgtgg gtgccaaaaa
     6181 ctaagggtga cccccgcaaa cccaacgaga taccagagga cgaacgggac tactacctgg
     6241 agcgtcgtta ccctgctttt gggaaccttg ttccgcgcga tgtggcttcg cgtgccgcca
     6301 aggaacgctg cgatgccggt tacggggttg gcccaacagg cctatcggtt tacctcgact
     6361 tccgcgatgc cattcagaaa aagggccgta agtacattga ggaaaactat ggcaacctat
     6421 ttgaaatgta cgagaaaatt acaggagaat cgccttacga ggtacccatg cgcatttacc
     6481 ctgctggcca ctacaccatg ggagggctat gggttgacta taacctgatg actaccatcc
     6541 ctggcctata cgccattggc gaagccaact ttagcgatca tggggcaaac cgcttaggcg
     6601 ccagctcact catgcaaacc tcgggcgatg gttactttat actaccctac accattgccg
     6661 attacttagc cgacgagata aaaacaccaa aaatacccac taatacccct gagttcgatg
     6721 cagccgaaaa agaggttaag gaacggattg aaaggttaat ggcaattaac ggaaccgaaa
     6781 cggcaaccag cttccacaag cggcttggaa agatcatgtg ggattactgc gggatggttc
     6841 gtaacgagga agggctgaag aaggcactta cgttgctcga tgagctaaag gatgaatttt
     6901 ggaaaaacct taaggttccc gatactgcca gcgagctgaa ccaagagctg gaaaaggcaa
     6961 atcgcgtagc cgactttctt gaactcggta aacttatggt ttacgatgca cttaaccgta
     7021 aggaaagttg cggggcacat ttccgggagg aaagccaaac tgaaacagga gaggccaaac
     7081 gcgacgacga gaactactca tacgttgcag cctgggagtt taccggcgat ggcaaggagc
     7141 caatactcca taaggaagag ctgaaatttg aatacgtaaa gcttcaagag cgcagctata
     7201 aatagcatct catcaaccat ataatactaa aggctatgaa ggtaaaactc aagatatggc
     7261 gacagaaaga ttcccgtagt aaaggaaaat tcgaggttta tgagctcgat aatgttgccc
     7321 ccgatatgtc gttccttgaa atgctcgact cgctgaacca gcgactggtt agggagggaa
     7381 aaagcccagt ggcgttcgac cacgactgcc gcgaggggat ttgcggttcc tgcggcatct
     7441 acatcaatgg tcgaccccat ggaaatgacc accgaaccac cacctgtgaa ctgcgtatgc
     7501 gccgctttaa ggatggcgat accattacca ttgagccctg gcgcgcagca gctttccctg
     7561 ttgtgaaaga cctagtggtt gaccgctcag cctttgataa gattatacaa gccggaggct
     7621 acattagcgt gaacgcaggt gccgcacagg atgccaatgc tattcctgta cccaaacaca
     7681 atgccgatgt tgccttcgac acggcatcgt gcattggttg tggggcatgc gtggctgctt
     7741 gtaaaaatgc atccgctatg ctctttgtgg ctgccaaaat cacccacctg gcacaatttc
     7801 ctcaagggcg ggttgaagca cccgagcggg ttaagcgtat ggtagccaaa atggatgaac
     7861 taggttttgg cagttgcact aatacacgtg cttgcgaggc tgaatgccca aagggtattt
     7921 ccattagcac aattgcaaag ctaaatcgcg aatatatcac cgctaaactt aaggactaaa
     7981 atgccaacta aaaaagataa atcgcccgac atactgcttg ttccggttga ttttggggac
     8041 tactatgaaa aggccattga attcgctcag aaattagccc caattacaga atcacaaatc
     8101 catttacttc atgttcttga tttaaaggat tggtggctcg atgaggttaa agaggaacgg
     8161 cttatcaatg aggcctttaa taaactgctt aacatatcgc gccagtttgg attacctgag
     8221 gactcaaagc tgatggtgca aaccggaaaa cggcaccata aaattgtgga atacgctgag
     8281 caggaaaatg tccgctttat ccttatggtc gataattacc cctttgccca ggaaggaaaa
     8341 agaattggat caaccctgga gaacgtgatt atgaaggcta ccaagcctgt aataacagta
     8401 aaaaccgttc ctgaaacaat ctttaaaaat atgcttgttc ccctggatct aactaaaaat
     8461 tgccgtgtaa aactatttaa ctctgttgcc cttgctctaa aattcgactc taaactaaat
     8521 atcgtatcgg taatttttgg ggatatcgac cctgaaaaaa gccgaataaa tgaaaagatt
     8581 gaaaagtatg cggctgttta tgctgaaaat cacatacccc atactaaaaa attgattcgc
     8641 aaggatgaag attttgcata tcaggaaata cttgattacg ccgatgaaat taagtgcgac
     8701 tccattataa ttatgacgca caaggaaaac gtgggctttg ataactacgt gggcgcattt
     8761 gctcaccaca ttatcaatga ggccaaggtt ccagttatca gccttaacaa tgcatcatca
     8821 acacaaacaa caagggattt actatcgtca attattgatc ccttaaatat tttttagggt
     8881 gttggctcac caatttgcct aaatatgtta taaatttgca gtgcaaacag gcgggagtag
     8941 ttcagctggt agaacatcag cttcccaagc tgagggtcgc gggttcgagt cccgtttccc
     9001 gctcaaacgt aaaggggtta acccctttac gtttttttat atagctttat ttagctacaa
     9061 ccttgagctt aaacctgttt atgtaaaaca attaaacggg ttgatgttca tttttttgat
     9121 taattttgca gtttagtaaa aacggattaa atggcagtaa aaagagtaaa gaaaacatcg
     9181 aacaccgagg atttacttac gtgtatagtt gatgctattc aagaaaaaaa ggggaaaaac
     9241 gttgtatcgc ttgagattgg agctttgccg aatgccgtat gcaagtactt tgttttctgt
     9301 aatgccgact ccaccacaca ggtagatgcc attgcaaaca atattgaaga taaagtgtta
     9361 gagaagctgg gcgaaaaggt ttaccgctcc gatggttacc aaaatgcaat ttggattgta
     9421 ctagactata tcgatatagt agtgcatgta ttccaaaccg aatggcgcaa ctactaccgt
     9481 gttgaagcac tttgggccga tgctaaagct atacagtacg aagaagttta acaataatac
     9541 gtaataatag aaatagtcct atatggctga gaacaacaat aacaacccta tgatgaacga
     9601 tgaaaacggc aaacccaaaa tgccacgatt caacgtttac tgggtttaca tcattataat
     9661 tcttggattt ttcctgatcc agtattttta ctctggtaaa acagctcagc taaccacatg
     9721 gcaggaggta aaaaaccaaa tgctggaaaa gcaggaaata agcaagctat tggtggtaag
     9781 gaacgctggc aaggtagagg tttaccttaa ggaagataag ctagagaagt acaaggatcg
     9841 gctaggacag gggtttggcg ctgttccaaa ggcagggcct cacttttact ttaccattgg
     9901 ttccattgaa acctttgagc ggcagcttgc cgaggcacaa accaatatag aggaaagtca
     9961 aaaaatatac ccggaatacg ttgaccggcc aaactattgg gccgatatac tttcatgggt
    10021 acttccgttc gtaatacttg ctgccatttg gatctacatt ttccgcagaa tttccagagg
    10081 cggtggttct ggaccgggag gcgggaatat tttctcggta ggtaagtcaa aggcacagct
    10141 gtttgataag gaaacacgcc caagggttga ctttaaggat gtggcagggc ttgaggaagc
    10201 caaaattgag gtaatggaaa tagttgattt cctcaaaaac cccaagaagt acactgagct
    10261 gggtggtaaa attcccaagg gtgctttact ggtaggccct ccgggaaccg gtaaaacact
    10321 acttgccaag gctgttgccg gcgaggccaa cgttcccttc tttagcatgt ccggttctga
    10381 gtttgtggag atgtttgtag gcgtgggagc atcgcgcgtt cgcgacctgt tccgtcaggc
    10441 caaggaaaag gctccatgca ttgtatttat cgacgagatt gatgccattg gccgagctcg
    10501 cggtaaaaac ccgagcttct ccggtaacga tgagcgtgag aacaccctta accaacttct
    10561 caccgagatg gatggatttg cctccaacca gggtgtaatc attctggcag caaccaaccg
    10621 cgccgatatt ttagaccgtg ctcttttgcg tgctggtcgt ttcgacaggc aaattcatgt
    10681 tgagttgccc gacctcaagg agcgtctcga tatctttaag gttcaccttc gccctcttaa
    10741 gcttgaggag aatctcgata tcagtttcct ggcaaagcaa accccaggat tttcaggtgc
    10801 cgatatagcc aatgtgtgca acgaggctgc tcttattgcc gcgcgtaaga ataaaaagta
    10861 tgttgagaaa caagatttcc ttgatgctat cgaccgaatc attggagggc ttgaaaagaa
    10921 gaataagatt atatcaaagg aggagcgtcg taccattgcc taccatgagg ctggacacgc
    10981 caccgttagc tggctgctag agcatgctaa tcctttgctc aaggtttcaa ttataccccg
    11041 tggcaggtcg ttaggcgcag catggtattt gcctgaggaa cgtcaaatca ctacaaccga
    11101 gcaactattc gacgagatgt gctcaaccct tggaggtagg gcagctgagg agataaactt
    11161 caataagata tcaactggcg ctctaagcga cctggaaagg gtaaccaaac aggcctacgc
    11221 catggttgct tactttggaa tgggcaagaa tcttcgaaat ataagctact acgattccac
    11281 cggccaggtt gaattcacat tttccaaacc ctacagcgag aaaaccgctg aacaaattga
    11341 cgaggaagtt aaagaaataa tcgataaagc ctacgaaaga gctaagcaga tacttaccga
    11401 gaaccacgaa ggtcatgttc agcttgctga gctactactt gaacgtgaag ttatcttcag
    11461 cgaggatttg gaaaagatat ttggcccacg gaaaacccta agccgtgagc aagaactggt
    11521 aaaagagctt gacgaggagg ctcacaagca aaattcagag agcaaagaaa accaaaccga
    11581 ataaaccatg gaccaaaact ggcaagaggt gttttccacc tcgcatacat ggcaggccga
    11641 aatggctaaa caaatcctcg aggaaaacga gattcctgct gttgtggtta accgtaaaga
    11701 ttccagctat ctttttggcg aagttgcagt gtatacagag cctgaaaacg ctgaaagggc
    11761 taaagaactt ttaaaagata tacagggttg agcgagtttt taaaacgtac cataagcgga
    11821 ttactttttg taatcgtact ggttggggca atttacattg gccacatcac ctttttcatt
    11881 ctttttttag ccattaccac tgccaccatg cttgagttct accatttagg acttaaagcc
    11941 aagttacggc cacaatcgtt acttggagta ttcattggga tggccttctt tatttggtcg
    12001 tacttctaca gctcaggccg gattgagcag ctaacgcttt acggttttgt acctttgctc
    12061 gtttcaattt ttgttgttga actttacagg caccagctaa aacccatgca gaacatagca
    12121 tttaccctgc ttggtatttt ctacattgcc ttaccgtttt cgttaatgaa cttcattacc
    12181 atcaatggct cctcctatag catggattac aacccgaaca tattactagg aatactcttt
    12241 ctggtttggg caaacgatac cggagcgtac attgttggtg tgagtatggg taagcataaa
    12301 atgattcccc ggatatcgcc caaaaagagc tgggaaggtt ttgttggggg tatagccacc
    12361 acgctactgg tagcatgggt tattgcaatg tttttcgatg aggtaagcct taagcactgg
    12421 cttgcaatag gactaattac aacctttatg gcggtactgg gcgatttagt ggaatcgatg
    12481 ttcaaacgca gtataggggt taaggattcc gggaaatttc ttcctggaca cgggggacta
    12541 ctcgaccgtt ttgacgcatt gttaatggta ctccccatgg tttatgttta ccttgaagta
    12601 atgatgatta tttaaatttc ttagtagata tgaaggttca caaggaggga tactcaattc
    12661 tttttgtaac actggcagtc ctggtggtac tatgcgccgt ttcctggtta atcctgccaa
    12721 aagcaatttc aattgttgca acggtagcat cggtgctgct aatggctttc ttaacccgat
    12781 ttttcagagt accttcaagg cacattgaaa cccgcctgaa tgcggttttg tcgcctgccg
    12841 atggcacagt tgttacaatt gaggaggtag aagagaacga gtaccttaaa acccgatgca
    12901 tacaggtttc aatattcatg tcggtttgga atgtccatat caactggttc ccagtaagtg
    12961 gcgttataaa gtactacaag tatcatcctg gcgattacct tgttgcatgg cacccaaaat
    13021 cgtcgtcgca taacgagcga accacggttg tggttgagcg tcccgatggt gttaaagtat
    13081 tactccgtca gattgctggc gcagtagccc gaaggattgt ttgctacgcc gaggtgggga
    13141 aacctgtaaa ccagtgctct gagctaggtt ttatcaagtt tggctcacgg gttgatgtat
    13201 tcctcccgct taacgccgac attaaagtag ccattggcca gaaggtgacc ggaaacctta
    13261 cggttattgc taccgtttag ccagccctta caccaacaca acctctacac ctttttcctc
    13321 aagctgttta acaaacagct cgttaacccc tttatcggta actatcaggt caacctgctc
    13381 gagctggcat atccgactaa aacccctacg gccgaatttt gagctatcgg ccaaaacaat
    13441 taccttttcg gcgctggcta tcatttgcct gttcagcgag gcctcaaggg cattggttgt
    13501 ggttagacca aattcggggt caataccatc aacacccata aagagcttgc tgcaccggaa
    13561 gccctcgagc atcttttcgg caaaggggcc aacaaccgat gtggcggtat gccttaccaa
    13621 gcctccaagc tgtaaaacct cagccactcc ccttacggca agctcacggg cggcaaccag
    13681 cgaggcgcat atgacagtaa tgttacgtcg ttcgcctatt tggcgagaaa actcattgat
    13741 ggtactccct gaggcaagaa taatactctc tccatcggaa actaaactgg ccgcataacg
    13801 cgatatgcgc tgcttctcct caacatatag ctgctctttc tctattacat gcctatcggc
    13861 tatgtacggg ttatttaccg atgcgctccc atgcgagcgg aaaagcagat tcctactttc
    13921 aagaagtttc agatcctttc gaatggtaac cgctgaaact cccagttgct cgctaagctc
    13981 aactaccgat acgtagcctg tctccttgag tttttcaaga atttttctat gccgttccgc
    14041 tatgctcaat gtcataccta ccaattttat ctcaaacata gtgatttttt aaatccgatg
    14101 ttttaagtag atacctaaaa aataatttca tttaatttcg ttttgtttcg ttttatttgt
    14161 tatttttacc aaaaatattt cgttgtatta ctaattctta cgatatgaat agagaacaga
    14221 tgctcagcca agctgtgtca aatgatcagg gatttgactt tgtaattatt gggggtggtg
    14281 caacaggtat aggaattgct cttgaagcag caacccgtgg ctatagaaca ctgctactgg
    14341 aaaaacatga tttcaccaaa tcaacctcga gtaaaagtac caagctggta catggcgggg
    14401 tgcggtattt ggctcaaggc gatattgctt tggtgcgcga ggcctgtatc gagcgaggaa
    14461 ggttactaaa aaatgctccc cacttagtaa aaaatcaaag ctttgtgatt ccaacctttg
    14521 gctggttcga tgagctgatg tacaccgttg gtctaacgtt ttacgattta ctggcaggca
    14581 gctacagcct tgggcgttcg ctcagggttt caaagaaaag agcgctggag tacattccaa
    14641 ccatcgaacc taaaaagatt actgccggtg tggtttacca cgatggtcaa ttcgacgatt
    14701 cgcgcctggc cataaatgtt cttcaaacag caaccgaaca tggtgccatg gtgcttaact
    14761 acatgaacgt ggttaaactc accaaaaaca ccaacggtat tgtcgacggg gttgtggctg
    14821 aggatttgga aacgggcagg agctacactt tccatggaaa agcaataata aacgcaaccg
    14881 gcgtttttgc tgacgacgta ctgcaaatgg acatccccgg ccaggcaaaa accatatcgc
    14941 caagtcaggg tgttcatgtt gtgctcgaca aaagcttttt acccggcaac catgccatga
    15001 tgatacccaa aaccgacgat ggacgggtac tatttgctgt tccgtggcac aaccgcattg
    15061 tggttggtac taccgatact ccgataccta acgcatcgct tgaacctgtt gcgcttgagg
    15121 aggaaataga atttattctg aacactgccg gccgatacct aaccaaagca cccaaacgaa
    15181 gcgatgtgct aagcgtgttt gctggattac gaccgcttgc ggcacccaca ggggaatcga
    15241 aaaagactaa ggaaatttcc cggagccata aaatcattgt aacccagtcc gagctcttta
    15301 ccatggtggg aggcaagtgg acaaccttcc gcaggatggc tgaggatatg gtgaataggg
    15361 ttgaatcggt taaaggttgg gagaaaagta aaacaaaaac ccggaacctg aaaattcatg
    15421 gttacacaaa caacgttaac catagcgacc cgctctatgt gtatggaacc gacaaggaaa
    15481 agatccttga actggcaaga gtagagcctg agcttggcga aaccctaagc cagagtcttg
    15541 gcattattaa agcccaagtg gtgtgggcag ttcgtaatga aatggctcgt aatgtggaag
    15601 actttttggc gcgtcgtacc cgatgcctgc tcctcgatgc tcgcgagagt atgcgcattg
    15661 cacctgcggt agcagctata atggcaaggg aattgaaccg tgataaaaac tgggaacgcg
    15721 aacaggtgga aaattaccta gctattgcac agaactacat tcttagctaa tttgtgaaac
    15781 cctaaaaaaa cctaaaatcg acctaaacct atggaaaagt ttataatggc actcgatcag
    15841 ggcacaacca gttcccgcgc cctgattttt gaccaaaagg ggaacattcg ttccatggcc
    15901 caaaaggaat ttcagcagat tttccctaaa ccgggctggg ttgagcacga ccccaacgag
    15961 atttggtcat cgcaggtggc ggttgcagcc gaagccatgt cgaagattgg gattaacggt
    16021 caaaatctgc atggcatagg cattaccaac caacgcgaaa ccactattgt ttgggacagg
    16081 agtaccggtg aacccatcta taacgccatt gtatggcaag atcgcagaac ttcatcgctt
    16141 tgcgatgagc ttagggcaaa gggattcact gacacgatac gacaaaaaac cggactggtg
    16201 attgatgcct actttagcgg aacaaaggtt aagtggatac tcgataatgt tcccggtgca
    16261 cgcgaaaagg caaatcgggg cgagttagcc tttggtacag tggacacctg gttagtgtgg
    16321 aagttaaccc gcggaagaac ccacgtaact gatgtttcca acgccagccg aaccatgctt
    16381 ttcaacatta acaccctaac atgggacaaa gagctgctcg atcttttaca aatacctgag
    16441 agcattttgc ctcgtgtggc ctcgtcgagc gaagtttacg accataccaa tactacattt
    16501 tttgctacaa aagtacctat tgcaggcatg gccggcgacc agcaggcagc cctttttgga
    16561 caaatgtgta ccagccaggg catggttaaa aacacctacg gaaccggttg ctttatactc
    16621 atgaacaccg ggaaaaaacc ttttctctca aaaaataatc tggtaaccac tattgcctgg
    16681 caagtgaatg gcgacaccta ctatgccctt gaaggtagta tttttattgg cggagctgta
    16741 gtgcaatggc tacgcgatgg tttaggtatc atcaaaagca gcgctgaggt ggaggagtta
    16801 gcccgccagg tgcccgacaa tggcgattta taccttgtac caagctttac cggaatgggt
    16861 gcaccgcatt gggatcagta tgcacgcggg ttgatgattg gtatctctcg cggaacaaca
    16921 cgtgcacaca ttgccagagc tgcactggaa agcatcgcct ttcaaaccat ggatgtgctt
    16981 gatgttatgg ctaaggatac cggcataaac cttacggaat tgcgagttga tggcggcgct
    17041 tcagctaata atctgctcat gcagttccaa tccgatgtgc ttggcatcaa cgttgtacgg
    17101 cctaaaacaa tagagacaac cgctatgggt gcggcttacc tggctgggtt agccacaggt
    17161 tactggaaaa atattgagga gattgccaac ctttggcaac tcgataaaac tttcactccc
    17221 ggcatgccct tagaaaccct acaaaaaatg aaggataaat gggccgatgc tgttgaaaga
    17281 gcaaaatcgt ggcataggta aaccaatata taatccccag attatgttat acttgcaatt
    17341 aaatctatga aaaaaataga caacaagaag aataacaaaa tggcaatagg gctttcaatt
    17401 ggtcttgtct taggagcaat agctgggatt actataggag tgttgctgca cgacatcgca
    17461 ctttggttat caattggaat aggaagcgga atggcatttg ggattgcaat aggtgtttta
    17521 atcggtagta gaaaaaaatt tgaataatga ctaatgacgc agtattaaag taataatgag
    17581 atttgtggtt agccaagcga aattgaacga tttccatgga acttggagtt aaattttcat
    17641 tttgaagccc tcgctgtagc gttttgtcac gtaattactt ttgtccatac gcataagtta
    17701 gtgtagacat ttttcagtac tggtcacagc cgctcgttta acgattcacg gttcacgttt
    17761 aacgaactaa acttcctcta actccatcat attccatcta ttccgactca ttccgactca
    17821 ttcctcaaaa aagtttccac cttcccattt ttactagcac gattgaatct gttaattcat
    17881 tgtacctatg ataaatgatt caaaacaata tacttaccaa aattttttca tactattgta
    17941 aaaactttga cgccatgaaa cacccatgta aggcagcttg cacaaacaca gatgaacaac
    18001 cgagagtttt ttgcgagctc gacgaagtca aactatgggt gttccatccg ctcgataaag
    18061 tttaataaaa gcaagacagt aacgtgttag taattaataa cataaatact ttgtacggat
    18121 gaaaaggttg atgattttat taggtatagc gttgactata ggtgttattt tagcgcccct
    18181 taaatcaaaa gcatggtcgg aacacccgct actggcatac ccggttctga gca
//
LOCUS       DAOOWJ010000053        18156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3094_length_18156_cov_8.097619, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000053 DAOOWJ010000000
VERSION     DAOOWJ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 18156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18156
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3094_length_18156_cov_8.097619"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..757
                     /locus_tag="PLW22_06710"
     CDS             284..757
                     /locus_tag="PLW22_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOU74432.1"
                     /translation="MKITLVAIGKTSEKYIAEGFDNYTKRLSRYCRFDFKVLPELKNT
                     KSMPVELQLQKEGDMILAEMENYQECILLDEHGKLYTSVEFAGLIEKRMNSGQRDMAF
                     FIGGPYGFDERAKAACHGKLSVSPMTFSHQLIRLIFAEQLYRAFTIIKGEPYHNP"
     gene            789..4451
                     /locus_tag="PLW22_06715"
     CDS             789..4451
                     /locus_tag="PLW22_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU74433.1"
                     /translation="MKQKIRYIPIILLAITFALLFIAEYGLSRIPYVQPITVQKKSIE
                     KIFERKTQKAFDLSKKLLEEIDSSCSSKSFQTCLSFINSLSDDDRFEIFILKNGKTCF
                     WSTNIDPQESPQRTGIAQLVKLNSAYYISFWNYNADSSLSARVLIHVSNEYPYQNKYL
                     VNSYTNDFDILSGFSISKQLESYCIPIRPKNFEPFYLVPTDKTGTVLKENFKTGLRYI
                     SLVLILVSLTFLLNQYFKKIHPAIKVMIFIAAIVAIRLVSLKTSFPGILPLTIFNPEL
                     YAHTWYNPSLGDFLINSVLVLVAAAFAFRNLSNYRVNIGLLGDRIISAISLLLTILCY
                     IAAHATMVSLVNHSTIPLDTSNIFNLNIYSVIGYLSVFFWVLAAITLNLLWLRINEKA
                     KGKRFIKYASLAALIVALPFFMLTDFSHISLFGYGWFLLVSVTLYWFCWVKNKAIGAK
                     ALLFLAAVSSIYITVIVSIYSRQKEVAVRKVLAVSLSSERDPLAETILPQLYQALTTD
                     AYARSCVKDIENKNVELYDYLRREYFKDYLNRYDLTATVCLENSILIDELGGKTNCNL
                     FFDNLISSYGIILPSSRFYYLSTQSGYIYYIGVLSYRFDDEVRNLYIELNSRPSWELL
                     GYPELLIEGKNKRNQLKGYSWAKYHKGKLVTKSGAYEYRLTVTDSDSLTGSYKFYNEN
                     RYNHLSYKPNQADTIVISRPRQEPLSSTASFAYILVFLLIILSLYLYLLGIKLSTQFT
                     SLKHKIGWAMVSIIILSMVLVAGATIYYNVKNFDDRNRSSLSEKLLSLQFELEHMLPY
                     MNKNRQDVEQLKEKLIDLSNTFYTDINIYDTSGVLLATSRSEVFDKNLLGRMMNPIAW
                     YQLHHLHNPKYLERESIGKAEFLSAYVPLLNPYGKTVAYLNLPYFTRQEELRNELYGI
                     IVAIINIFALLAMLSISLVVAIASELTKPLELIRESMSRVNITGNNATIEYAGNDEVG
                     QLIAEYNRMVIELARSAEELARSQRETAWREMAKQIAHEVKNPLTPIKLSLQHLVKAK
                     REGLPNWDERFEQFAQSLTEQIKALTVIANEFSSFAKLPTAKREPVNLIAVINDVITL
                     YSSYKHIRIVLNNAAGEPFVFADRDQLLRVFNNLVKNGIQAIERGKTGEVSISISKTS
                     SSMVRVDVSDTGIGIPKHAREKIFTPNFTTKSGGTGLGLAITREIILNFGGNIHFTST
                     ENIGTTFTVDLPLYEA"
     gene            complement(4456..5739)
                     /locus_tag="PLW22_06720"
     CDS             complement(4456..5739)
                     /locus_tag="PLW22_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU74434.1"
                     /translation="MKKGFGSFGKSFWTAITMEFFERGSYYGVMSILSVYLVLSTDQG
                     GLGFTKEKVGIIKSVITPLLYILPIVAGAIADRFGYRKTLFFAFAVMSSGYFLTSIST
                     SYSAVFLSLTLMAIGAGTFKPIISGTIARTTNESNSSLGFGIYYWSINLGAFLFPLLL
                     VPYLKSLGWNYILLMAAFGTGWLLLLNFFVFREPSRPQSQKNLVQVFADMLLVLKDYK
                     FILTIVIYSGFWILYFQMYDSVLWFLKERIDMTPVNNAVNSFLTIFVDNPSWRFDAEH
                     VTVVNAGTIILLQLIVSAILKNAKPLQTMIVGISFGTLGLGLLAISTYAWVFILGLVI
                     FTIGEMTAHPKFISYIGLIAPQDKKALYLGYSFLYGVIGSGIGGVLGAFAYVKFVTNM
                     NRPELFWLMFCGIGVLTIIGLILFNRFVLKGSKPS"
     gene            complement(5859..6386)
                     /locus_tag="PLW22_06725"
     CDS             complement(5859..6386)
                     /locus_tag="PLW22_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74435.1"
                     /translation="MKKFTLLSIVAVCFWAIAVNAQDEKLQKLEAKVEAAKAKVEKAD
                     AKIYSADSLITEGKRIMQDASVDIRALEAEQKQMEKAHFNEIKPLEKKLRSKDKDEVN
                     SAKAEIKESENKFKEEVKQWNAKYKEALKVYENGQKMVAKGEENMKKAKGEKKLAEDA
                     LKEAQKAYEKASKTK"
     gene            complement(6523..8502)
                     /locus_tag="PLW22_06730"
     CDS             complement(6523..8502)
                     /locus_tag="PLW22_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU74436.1"
                     /translation="MKTFIKATLLSAWSIIGLLATTQAQEFSKLTPKQIGDKITLEYA
                     ISGEAVGQQFTVSPFYSIDGGKSFLPLKSVKGNVGNNIPGGRNQIIVWDVLKDLKELD
                     SDVVFKLEGVSKSVVPLQDDFRDVLFKLESLNRTPDGNVELILSITNNGPTRDLKMIN
                     GLITITDFNKRKYDAQKGKIAEVEGNERYSTPQRTIKKGENVKARFLFERVPAEVDRA
                     MRLDIGIELITDETYGIDLKIGKLQFRDLPVSTVQTTGMRVEQTKKLQLASSPEHFTI
                     RKVEVVDNNPPVIELISPANIPLVGKEATRGRPYAQSSIGMDDKRLRSLATGEELATT
                     DEFTMVKAKVTDESGIYDVTVNGISTEPSKDGVFQVKVPLEIGRNEIVIRAVDLRQNS
                     IEKKFFVLRKSPSGKVAKGDTEELDLVFDSPKTAPRYFALIMGANDYADDAITDLDNP
                     IQDATKLYNVLVNRYTFDPSNVIFLKNPTREQMINAMDWLTRKVTKYDNLLVFYAGHG
                     FWDKETDFGYWIPTDSKSNSTANWLANSQIKDYVAAIKSKHTLVIADACFGGSIFRSR
                     KAFEEGSQSVKKAFDAPSRKAMTSGNLTEVPDRSVFLEQLVDKLNNNTKSYVTAEELF
                     ASIRNVVLSSSPVTPMYGDIKDAGDQGGDFIFVLK"
     gene            complement(8537..9565)
                     /locus_tag="PLW22_06735"
     CDS             complement(8537..9565)
                     /locus_tag="PLW22_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74437.1"
                     /translation="MNWKAGFTLILLFFIGINAYAQDKERFQKIFEKETSGETDNQKR
                     FKNVSRLSYYPDTLPSWFFNPHANGMGYYAIGVSDPDMVLDEAKKQAIIRAKSIALLN
                     EECKIQYFKDIYTDAKEVQRYTNLNERFDIYFKLTAKRKVSDAMFAVLDTHVTRYNEF
                     VVLVSYNPQRVNESDTLYPLMASASAFFVSASYDGAEDNQAEYEMKNYLLTPDQKVTG
                     AEFLFREKGNKFLSVSSFNGEQLEYPAFPYSYTSPDATSRNQVLVTYNGLWSILSRQM
                     LFTLMLNTQPYSVKLKNVDQQYTSGVTRLAREIASFSSTLEINGIKFVNDTLKFDLNL
                     KGQTNSMW"
     gene            9776..11245
                     /gene="rmuC"
                     /locus_tag="PLW22_06740"
     CDS             9776..11245
                     /gene="rmuC"
                     /locus_tag="PLW22_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOU74438.1"
                     /translation="MEVIIGLAGVGVGALLIYLILKGKTTNAAKQQAYIDELTAKLNQ
                     LSTEADVLAERNRHASETISTLQATLAQRDEERIALTAKVSSLEASLQSATKTAAELR
                     QEIEKLLVDIDTKQHQLEQARYRGVELEKENIYLKESLEKQKKDLEAIGEQFTKEFKL
                     LADQILEDKSQRFTKMNAETIDRLLKPLDENIKQFQKQVQEVYEKESRERFSLGEKVK
                     ELSELNRTISQEAKNLTEALKGQVKTQGNWGEMILESILSKSGLVKGREYTVQESITD
                     EEGKRFQPDVIISYPDNRKVVIDSKVTLVAYERYCSASNPTEQQAAVNDLLQSVKRHI
                     DGLSAKSYDELVDSLDFVMMFIPIEPAFMLVMERDPAIWNYAYEKRVLLISPTNLIAA
                     LKLIYDLWQREYRNRYAEEIAKKGGQLYDKFVGFLEDFRKIGDSLTSAQKKYEEAFGK
                     LKDGRGNLVVQAQQLKELGAKAKKSIPDDFLPIDNELDE"
     gene            11321..12244
                     /locus_tag="PLW22_06745"
     CDS             11321..12244
                     /locus_tag="PLW22_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU74439.1"
                     /translation="MISVCIPVYNFDVTELVGELIAQANRLQYPIEILIFDDHSHSYF
                     RNRNSQLASHSLVNYLEFDFNIGRSKIRNRLADVATGQWLLFLDCDVMVDDPNYLKRY
                     IENLPNAKVVCGGRKYGPKPFRPELLLRWKYGVKRECRSAYSRQLKPYSSFISGNFLI
                     EYDTFHSIRFNEELSGYGHEDTLLGLDLKKHKVDILHIDNPTIHMGLDSNQEFLEKTD
                     QGVKNLVKILHIMPCMRKDIEKSITLLKVFRFLRKVGMLYPLRWVSRVYTPLLRKQLC
                     GSRPSILLMDLYKLTLLAKLYTKGWRNQSQP"
     gene            complement(12251..13558)
                     /gene="der"
                     /locus_tag="PLW22_06750"
     CDS             complement(12251..13558)
                     /gene="der"
                     /locus_tag="PLW22_06750"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOU74440.1"
                     /translation="MGNLVAIVGRPNVGKSTFFNRLVGWRHAIVDEIAGVTRDRIYGK
                     SDWNGKEFSVIDTGGWVENSDDIFEEEIRKQVLIAIDEADIITFLVDAQEGITDMDIA
                     VAEILRRSSKPVYLVANKVDNNAIRMQAGEFYSLGLGDPWCISSINGSGTGDLLDDIV
                     KHFTDIDESAEQNEVPRIAVVGRPNVGKSSLINTFIGEERNIVTPLAGTTRDAIDTHY
                     RKFGHDFIIVDTAGLRRKTKVKEDLEFYSVMRSVRAIESSDVCLLLIDATQGIERQDM
                     NIFSLIVRNKKGLVLVVNKWDLVEKNNKTVDEYTKLIKERIAPFNDVPIIFASALTKQ
                     RVFKVIEAAMQVYENRKRRISTSELNNFLQETLQIKEPPVYKGKLVKIKYATQLPTKT
                     PQFAFFCNLPQYIKDPYKRFIENRLREKFNLTGVPIQIYFREK"
     gene            complement(13601..14482)
                     /gene="era"
                     /locus_tag="PLW22_06755"
     CDS             complement(13601..14482)
                     /gene="era"
                     /locus_tag="PLW22_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOU74441.1"
                     /translation="MSHKSGFVNIIGNPNVGKSTLMNALVGERISIVTSKAQTTRHRI
                     MGIVNSDDFQIVYSDTPGILKPAYKLQSAMMRFVESALEDADVILYITDTVETPNKNQ
                     EFLDKIQQLDIPIILAINKVDLSTQEKVSLLIEQWGQMMPKADILPISALHGFNTDAL
                     VNMIVERLPEGPEYFPKDTLTDKTLRFFAAEIIREKILLNYKKEIPYSVEVEIEEYKE
                     SETIDRIRAIIYVDRESQKSIIIGSQGRMLKKVGTEARLELETFLGKKVFLEIYVKVD
                     PNWRNDEGKLKRFGYPL"
     gene            complement(14502..15707)
                     /locus_tag="PLW22_06760"
     CDS             complement(14502..15707)
                     /locus_tag="PLW22_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOU74442.1"
                     /translation="MRKESMNRDVKREKRSNSPEEPARVYRPRTLTLKKVDANDQSDS
                     KPEKRVSPRSDEHKPEPKRRSEDSRRGDFRKPKSDNSEQPKSYRPRSWKKDSDSREHE
                     PRETSHERSSRPSRDERKHESGRKNDGFKLVKHTRYNESKSKEYTRDNAKAEGMVAKK
                     TADELIRLNRYIANSGVCSRREADELIANGMVTVNGKVVTELGTKVRRDDVVMYKGKR
                     LTAERKVYILLNKPKDYVTTVDDPHAKHTVMELIEGACDVRVYPVGRLDRNSTGVLLL
                     TNDGDLTTRLTHPSFKKRKIYHVGLDRNVRPEDMNKIFEGVELDGEKIQVDAIDYVDG
                     SDGSEVGIEIHSGQNRVVRRIFESLGYKVIKLDRVYFAGLTKKNLPRGKWRFLTEKEV
                     NMLKMNRFY"
     gene            16298..17329
                     /locus_tag="PLW22_06765"
     CDS             16298..17329
                     /locus_tag="PLW22_06765"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOU74443.1"
                     /translation="MNRVVKMKRIFTYLALLILIFINGRCTKDNESPLPVSYGILFYS
                     DRDGDNEIYSMNADGGGLKKLTDNGYNDCNPQWSPDGSKIVFESNRDGNREIYVMNAD
                     GSNQIRLTYYNLNDWLPQWSPDGKQILFIRDLGFGNYEIFTMDADGNNITNLTNIALQ
                     DYNPHWSPDGSKIAFERKINNTMEIFVMNKDGSNQIRLTDNSANDYNPRWSPNGDKII
                     FSSNRDGNGEIYVMNANGSNQTRITENNGEDSWPQWSPDGSKLIAARYTSGNLEVYLM
                     NLDGTGYLRLFGTLSDIEYPQWHHDGNFIILAARKYNDWEIYTINTNKPEELNITNNT
                     GNDHLYFYR"
     gene            17680..18069
                     /locus_tag="PLW22_06770"
     CDS             17680..18069
                     /locus_tag="PLW22_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74444.1"
                     /translation="MLLVIIIAVCYMYEKVRESKYSVGGDCVYIEKEICPKFSLLFID
                     SSCITIKEIKLDNKTIFKLDTTNKLKQFNHNFISGKKKIELIIKDKSVSSDSVCLDDW
                     CEYYWLTIEKDKENNICKINIKPVTLD"
BASE COUNT         5203 a         3983 c         3819 g         5151 t
ORIGIN      
        1 aattattttt gtccatacac ataagtttgt gtagacattt ttcaggacgg gtcacgagaa
       61 acgttacaca tgaggttttt ttagtgtcaa ccatcttata attcctgcac aactatcatt
      121 ctgcgatgtt tgcaaaatgc catctaaact gtgccattta acccacgcct atcccccatc
      181 aagcaataaa catttgcaca acctgtaaca actatatcaa gttttaatta attttatggt
      241 ttctgtgtta acaggaaacc ataaaatctt taccattttg ccaatgaaaa taactcttgt
      301 agcaatagga aagacaagtg aaaaatacat tgccgagggg ttcgataatt acacaaaacg
      361 gcttagccgt tactgcaggt ttgactttaa agtccttcct gaactcaaaa ataccaaatc
      421 gatgccggtt gagctgcagc tgcaaaagga aggtgatatg atacttgccg agatggaaaa
      481 ctaccaggag tgcatactgc tcgatgagca cggaaagctt tatacatcgg ttgaatttgc
      541 gggtctaatt gaaaaacgta tgaactccgg ccaacgcgat atggctttct ttattggtgg
      601 gccctacggt ttcgatgagc gggcaaaagc tgcatgtcac ggaaaactct ccgtttcgcc
      661 tatgaccttt tcgcatcagc ttattagatt gatatttgcc gaacagcttt acagggcctt
      721 cacaatcatt aagggggaac cttaccataa tccttgattt aggctcgatt tttgctataa
      781 tttaagtaat gaaacaaaaa atacgctata taccaataat ccttcttgct atcacgttcg
      841 cactcctatt tatcgcggag tatggactaa gtagaatccc ttatgtgcaa cctataacag
      901 ttcaaaaaaa atccatagaa aaaatctttg agaggaagac ccaaaaagca tttgacctat
      961 cgaagaaact gttggaagaa attgatagtt cctgctcatc aaaatcgttt caaacatgcc
     1021 ttagctttat taactcactg tcagatgatg accgttttga gatttttatt ctaaaaaatg
     1081 ggaaaacctg cttttggtca acaaatatcg atccccagga gagtccacag agaacaggta
     1141 ttgctcagct ggtgaagctg aacagcgctt actacatctc attctggaac tacaatgccg
     1201 actcttcgct atccgcaagg gttcttatcc atgtatcgaa cgaataccct taccaaaata
     1261 agtatcttgt aaacagctac accaatgatt ttgatatact ctcagggttt agcatatcaa
     1321 agcaattgga atcgtactgc attccaataa ggcctaaaaa ctttgagccc ttttacttgg
     1381 ttccaaccga taaaacaggg actgtcctaa aagaaaactt taaaacgggt ttacgataca
     1441 tttcgctggt tttgatacta gtttcgctga cctttttgct aaaccaatat ttcaaaaaaa
     1501 ttcacccggc aataaaagtt atgatattta tagctgcaat tgtggctatt agactagtta
     1561 gtcttaaaac atcgttcccg gggatacttc cactaactat ttttaatcct gaactttacg
     1621 ctcatacctg gtacaacccc tcgcttggcg atttcttaat aaattcagtg ttagttttgg
     1681 tagcggcagc atttgctttc cgtaacttaa gtaattacag ggtaaatatt ggcttattag
     1741 gcgatagaat aatttcggct atcagtctgc tgctcacaat cctttgctat attgctgccc
     1801 atgccacaat ggtatcgctg gttaatcact ctacaattcc tctcgacaca tcaaatatct
     1861 tcaacctcaa catttactcg gtaataggat acctttcagt gtttttctgg gttcttgctg
     1921 caattaccct caacctgctt tggcttcgga ttaacgaaaa ggccaaaggt aaacgtttta
     1981 ttaaatacgc atcgctcgct gcacttattg ttgctctccc attttttatg ctaaccgatt
     2041 ttagccacat atcccttttt ggctatgggt ggttcttgtt agtttcggta accctttact
     2101 ggttctgttg ggtaaaaaac aaagcaattg gagccaaggc tctgcttttt cttgcagctg
     2161 tttcgtcaat ctatatcact gtaatagttt caatctacag ccgacaaaag gaggttgccg
     2221 ttcgaaaagt tcttgcagta agcttgagta gtgagcggga tccactggct gaaaccatac
     2281 tgccacagct ttaccaggcg cttacaaccg atgcctacgc ccgtagctgc gttaaggata
     2341 ttgagaacaa aaacgttgag ctttacgatt acttacgccg agaatacttt aaggactacc
     2401 ttaatcggta cgacttaacc gccacggttt gcttggaaaa ctcaatactt atcgacgagt
     2461 tgggaggcaa aacaaactgc aatctatttt ttgataacct gatttcaagt tacggaatta
     2521 tcctacccag ctcaagattc tactacctat caacccaatc gggctacatc tactacattg
     2581 gcgtgttgag ctaccgattc gacgatgagg ttcgcaacct ttacatagag ctgaatagcc
     2641 gcccgagctg ggaactcctg gggtatcctg aactacttat tgaagggaaa aacaaacgta
     2701 atcagctaaa gggttactcg tgggctaagt atcacaaggg aaagctggtt acaaaatcgg
     2761 gggcgtacga gtacaggtta acagtaacag attccgattc ccttacgggt tcctacaaat
     2821 tttataatga gaataggtac aaccacttgt cctataaacc aaaccaggcc gatacaattg
     2881 taattagccg acctcgtcag gaacccctta gctcaacggc ttcgtttgca tacatcctag
     2941 tttttctcct gataattcta tcgctttacc tatacctgct tggaataaag ctaagcaccc
     3001 aatttaccag cctgaagcac aaaattggat gggcaatggt gtcaataatt attctatcga
     3061 tggtgcttgt ggctggagcc acaatttact ataatgttaa gaactttgat gacagaaata
     3121 gaagtagcct aagcgaaaag ttgctctcgc tccagtttga actggaacac atgcttccct
     3181 acatgaacaa gaacaggcag gatgttgagc agcttaaaga gaagctgatt gacctttcta
     3241 acacatttta cacggacatt aacatttacg atacaagcgg agttctgttg gccacatcgc
     3301 gttctgaggt ttttgataaa aacctattag gccgaatgat gaaccccatt gcatggtatc
     3361 agcttcatca cctacacaat cccaagtatt tagagcggga gagcataggg aaagccgaat
     3421 tcctttccgc ttacgtaccc cttttgaacc cctacggtaa aacagttgcc tacctcaact
     3481 taccctactt tacccggcag gaggaacttc gcaatgaact ctatggaatt attgtggcaa
     3541 ttatcaacat attcgcactg cttgccatgc tttcaatttc gttggtggta gcaatagcct
     3601 cggaactaac caaaccgcta gagctgatcc gtgagagcat gagccgggta aacattaccg
     3661 gcaataatgc caccatagag tatgcgggaa acgatgaggt aggacagctc attgccgagt
     3721 ataaccgaat ggtaatagaa ttagctcgga gcgccgaaga acttgcccgt agccagcgcg
     3781 aaacagcctg gcgcgaaatg gctaaacaga ttgcccacga ggttaaaaac cccttaacgc
     3841 caataaagct tagcctgcaa cacctggtta aagccaagcg cgaaggtttg ccaaactggg
     3901 acgaacgttt tgagcagttt gcacaatcgc taaccgaaca aataaaagcc ctaacggtta
     3961 ttgccaatga gttctcgtcg tttgcaaaac ttcccaccgc aaaacgtgag cccgttaacc
     4021 ttattgctgt tataaacgat gttatcaccc tttactccag ctataaacat atcaggatag
     4081 tgcttaacaa tgcagcgggc gaaccgtttg tttttgccga cagggatcaa cttttaaggg
     4141 tgttcaacaa ccttgttaaa aatggcatac aggccattga gcgtggtaaa accggtgagg
     4201 ttagcataag tatatcaaaa acatccagca gtatggtaag agttgatgtt tccgatacgg
     4261 gcattggcat accaaaacat gctcgggaaa aaatctttac gcctaatttc acaaccaaat
     4321 cgggaggaac cggtttaggg ctagccatca cacgcgaaat aattttgaat ttcggaggta
     4381 acatccattt tacctctacc gaaaacattg gcacaacctt tacggtcgat ctcccactct
     4441 atgaagctta actggttagc tcggctttga accctttaga acgaacctgt taaacaggat
     4501 taaaccgata atggtcaata ctccaattcc gcaaaacatc aaccagaaca actcggggcg
     4561 gttcatgtta gttacaaact taacgtaagc aaaagcacca agaaccccac caataccgct
     4621 acctattact ccgtaaagga atgagtagcc caggtaaagc gcttttttat cctgtggcgc
     4681 aattaaacct atataggaaa taaactttgg gtgggcggtc atctcgccaa tggtaaaaat
     4741 cactaatcct aaaataaata cccatgcata ggttgaaatg gccaaaaggc caagtcccag
     4801 tgtcccaaac gatattccaa ctatcatggt ttgtaatggt ttggcattct ttagaattgc
     4861 cgatactata agctgcaaaa gtattatggt tcccgcatta actaccgtaa catgttcagc
     4921 atcaaacctc cacgatggat tatcgacaaa aatggtcaaa aaagaattta cagcattgtt
     4981 aaccggggtc atgtcgatac gctcctttaa gaaccagagt acagagtcgt acatctgaaa
     5041 gtaaagaatc cagaaacctg agtatattac aatggttagt atgaacttat aatcttttag
     5101 aaccaaaagc atatcggcaa aaacctgaac aaggtttttc tggctttgtg gccttgaggg
     5161 ttcacggaac acaaagaaat ttaataatag cagccaacct gtcccaaatg cggccatcag
     5221 cagaatgtag ttccaaccca acgatttaag gtaagggact aagagtaatg ggaaaagaaa
     5281 ggccccaagg tttatagacc agtaatatat tccaaatcct aacgatgagt tcgactcgtt
     5341 ggtggttcgg gctattgttc cggaaattat tggtttaaag gttcccgcac ctattgccat
     5401 gagcgtaaga cttaggaata cagcagagta tgaggtgcta atgcttgtta ggaagtaccc
     5461 tgaactcatt acggcaaagg caaagaatag tgtttttcgg tagccaaatc tatcggctat
     5521 ggcccctgcc actatgggga gtatatatag taacggcgta ataacgcttt ttatgattcc
     5581 taccttttcc ttggtaaaac ccaatccgcc ttgatcagta ctaagaacca ggtaaaccga
     5641 aagaatcgac atcacaccgt agtatgagcc cctttcaaag aattccatgg taatggcagt
     5701 ccaaaaactt ttaccaaacg agccaaagcc ttttttcata gttactcaca attaattttc
     5761 gcaatattca acttttttcc catttgtaag catgataaaa atcaaattgt aaaaaaaggg
     5821 ggatattatc cccccagcct ctatattgca agtaagtttt actttgtttt gcttgccttt
     5881 tcgtaagcct tttgggcctc cttgagggca tcctccgcaa gtttcttttc gcccttagcc
     5941 tttttcatat tctcctcacc ttttgccacc atcttttgac cgttctcata aaccttgaga
     6001 gcttccttat acttggcatt ccattgctta acttcttcct taaacttatt ctccgattcc
     6061 tttatttcag ccttagcgct atttacctca tccttatcct tggagcggag ttttttctcc
     6121 aatggcttaa tctcgttaaa atgggccttt tccatttgtt tctgttcagc ttcaagcgct
     6181 ctaatatcaa cggatgcatc ctgcataatt cttttccctt cggttatcaa gctgtcggct
     6241 gagtaaatct tggcatcggc tttttcaacc ttagccttgg cggcttcaac ctttgcctca
     6301 agtttctgta acttttcatc ctgggcgttt acggcgatag cccaaaagca aactgcaaca
     6361 atgcttaaaa gagtaaattt tttcatgcta aatagggtta ttatgtttaa cattcattta
     6421 cttaccagag gaattcattt gcaaattctt aaaattctta cgaaaaagca agttacaggt
     6481 tacaaaaagt cggggcagtg taaagccccg acttttgata aactatttga gaacaaaaat
     6541 aaagtcacca ccctgatcgc cggcatcttt aatatcgccg tacattgggg ttacaggact
     6601 gctacttaat accacattgc ggattgaggc aaaaagttcc tcggcagtaa catagctctt
     6661 ggtgttgttg tttagcttgt caacaagctg ctcaaggaat acgctacggt cgggcacctc
     6721 ggtaaggtta ccgctagtca tagccttacg gcttggggca tcaaatgctt ttttaactga
     6781 ttgtgacccc tcctcaaaag ccttacgtga acggaaaata cttccgccaa agcatgcgtc
     6841 ggcaatcacc agggtatgtt tcgatttgat ggcagcaaca taatccttta tttggctatt
     6901 agccagccag ttggcagtac tattcgattt tgaatcggtg ggtatccagt aaccaaagtc
     6961 ggtttcctta tcccaaaaac catggccagc ataaaatacc aataggttat cgtacttggt
     7021 taccttacgg gtaagccaat ccatggcatt tatcatttgc tcacgggttg gattttttag
     7081 gaagattaca ttgcttgggt cgaatgtata gcggttaacc agaacgttat aaagcttggt
     7141 ggcatcctgt atgggattat ccaaatcggt aatggcatcg tcggcatagt cgttggcacc
     7201 catgattaga gcaaagtaac gaggggcggt ttttggagaa tcaaaaacca ggtcaagttc
     7261 ttcggtgtcg ccctttgcta cctttccaga gggtgatttc cgaagcacga agaatttctt
     7321 ttcaatgcta ttctggcgta ggtcaactgc gcggattaca atctcgttcc tgcctatctc
     7381 caaaggaacc ttaacctgga atactccatc ctttgagggc tcggtagaaa taccgttaac
     7441 ggttacatcg tatatcccgc tctcatcggt aaccttggcc ttaaccatgg taaactcatc
     7501 agtggtggca agttcctcac cggtagcaag ggagcggagc cgcttatcgt ccatcccaat
     7561 ggacgactgt gcgtaggggc gaccgcgagt tgcctctttg cctacaagcg ggatgttggc
     7621 tggcgaaata agctcaatta ccggcgggtt attatcaact acctccacct tgcgaatggt
     7681 aaaatgctca ggcgatgacg ctagctgaag ttttttggtt tgttcaacac gcatccctgt
     7741 agtttgaacg gtactaacgg gaaggtcgcg gaattgtagt tttccaattt tcaggtcaat
     7801 accgtaagtt tcatcggtta tgagttcaat acctatatcg agcctcatgg cgcggtcaac
     7861 ctcggcgggt acacgctcaa acaggaagcg ggccttaacg ttctcgccct tcttaatggt
     7921 tcgttgtggg gtggagtaac gctcgttccc ttcaacctct gctattttac ccttctgggc
     7981 atcgtattta cgtttattga aatcggtgat ggtgattaac ccgttaatca tttttaaatc
     8041 gcgggtgggg ccgttgttgg taatggagag tatcagctca acgttcccat cgggtgtgcg
     8101 atttaagctc tccagcttaa agagaacgtc acggaaatcg tcctgaaggg gcacaacgct
     8161 ttttgagacc ccctcgagct tgaaaaccac atcggagtcg agttccttga ggtcttttag
     8221 tacatcccaa acaattattt gatttctacc accgggtata ttgttcccca cattaccctt
     8281 aacgctcttg agcggtaaga acgatttccc cccatcgatg gagtagaagg gcgaaactgt
     8341 aaattgctgt cccacagcct cgccggaaat ggcgtattca agagtaattt tatcgcctat
     8401 ctgtttaggc gttagcttgc taaattcttg cgcttgcgtt gtagcaagta aacctatgat
     8461 actccatgct gagagtagcg ttgctttgat aaatgttttc attgcgccat ctattttagt
     8521 tttacatata aagcttttac cacatgctat tggtttgacc cttaaggttc aggtcaaact
     8581 tcaaggtatc atttacaaat ttgatgccgt taatttcaag ggtggatgaa aatgatgcta
     8641 tttcacgtgc taaccgggta acgccgctgg tgtactgttg gtcaacattt ttcagcttaa
     8701 cgctgtaggg ctgagtgttt agcatcaggg taaaaagcat ttgcctcgat agaatgctcc
     8761 ataaaccatt gtaagttacc agaacctgat tccgcgatgt ggcatcgggc gatgtgtagg
     8821 agtaagggaa tgccggatat tcaagctgct caccgttaaa gctcgatacg cttaggaact
     8881 tattaccttt ctccctgaat aggaattcgg caccagtaac cttctggtcg ggagtaagca
     8941 ggtagttttt catttcgtac tcagcctggt tatcctcagc tccatcgtag cttgccgaaa
     9001 caaaaaacgc cgatgcgctg gccattagtg ggtaaagagt atcggactca ttaacccgtt
     9061 gtgggttata gctaaccagc accacaaact cgttatagcg tgttacatgt gtgtcgagca
     9121 cagcaaacat agcatcggaa accttgcgtt tggctgtcag cttaaagtag atatcaaacc
     9181 gctcgttaag gttggtgtag cgttgaactt cctttgcatc ggtgtaaata tccttgaagt
     9241 actgaatttt acactcctcg tttaagagcg ctattgactt tgcccggatt atagcctgct
     9301 ttttggcttc atcgagcacc atgtcggggt cggaaactcc aatggcatag taacccatac
     9361 cgttagcgtg tggattaaaa aaccatgagg gtaaagtatc ggggtagtag cttaggcgcg
     9421 atacattttt aaaccgcttc tggttatctg tttcaccgga ggtttccttt tcaaaaatct
     9481 tttgaaaccg ttctttatcc tgtgcataag catttatgcc gataaagaat agtaaaatta
     9541 aagtaaaccc tgctttccaa ttcatcgcac tcatagtatc aagtatctaa aattacaaaa
     9601 attcttgctc gttaggggta gaaatgtcgc tctttttaca ccaacaattt aaaaaagttt
     9661 gataaacagc ccaaacttgt tgacaacttt actaaataca taggagtgga aaagcagttt
     9721 ttagtaactt tatagactgt aacgtttttt ctaatagttt gataaacata aatttatgga
     9781 agttattatc gggttagccg gagttggagt aggcgcattg cttatttacc tgattttaaa
     9841 aggcaaaaca actaatgcgg caaaacagca agcctatatt gatgagttga ccgcaaagct
     9901 gaaccagttg agtaccgagg ctgatgtatt agccgaaagg aaccgacatg ccagcgaaac
     9961 cataagtaca ctacaagcca cacttgcaca acgcgatgag gaaagaatag cgctaaccgc
    10021 aaaggtttca tcgcttgaag catcgctaca gagtgccact aaaacagccg ctgaactgag
    10081 gcaggaaatt gagaagctac tggtagacat cgataccaaa cagcatcagc ttgaacaggc
    10141 acgttacagg ggagtagagc ttgaaaagga gaatatctac ctgaaagaat cacttgaaaa
    10201 gcaaaagaag gacttagagg caattggtga gcagttcacc aaggagttta agctcctggc
    10261 cgaccaaatc cttgaggata aatcgcaacg gtttaccaaa atgaacgcgg aaaccatcga
    10321 taggctgctg aagccgctcg acgagaatat aaagcagttc caaaagcagg ttcaagaggt
    10381 ttacgagaag gaaagccgtg agcgtttctc gctgggcgag aaggttaagg aacttagcga
    10441 gcttaaccga accatcagcc aggaggccaa gaacctaacc gaggcgctga aggggcaggt
    10501 gaaaacacag ggaaactggg gcgaaatgat tcttgagtcc attctatcca aatcggggct
    10561 ggtaaaaggg cgtgagtaca ccgtgcagga gtcgataacg gacgaggagg ggaagcgttt
    10621 tcagcccgat gttatcattt cataccccga taaccgcaag gtggtaattg actcaaaggt
    10681 gacactggtg gcctatgagc gttattgctc ggcatcgaac ccaacggagc agcaagctgc
    10741 tgtaaacgat ttgctacaat cggttaagcg acatatagat ggactatcgg caaaaagcta
    10801 tgacgagctg gttgattcgc tcgattttgt aatgatgttc atcccaattg aacccgcatt
    10861 tatgcttgtg atggaacgcg acccggcaat ctggaactat gcctacgaaa agcgtgtttt
    10921 gcttatcagc cctacaaacc tgattgctgc tttaaagctg atatacgacc tgtggcagcg
    10981 cgagtaccgc aaccgttacg ccgaggaaat tgccaaaaag ggcggccagc tctacgataa
    11041 gtttgtgggt ttcctggaag atttccgtaa aataggcgat agcctcacat ctgctcaaaa
    11101 aaaatacgag gaagcgtttg ggaagctaaa ggatggcagg ggcaaccttg tggttcaggc
    11161 acagcagcta aaggaactag gagcaaaggc aaagaagagt atccccgacg atttcctgcc
    11221 tattgataac gaattggatg agtaaggcaa acaaaaattt cgatttcatc gtattacaat
    11281 gaggcaattt gtgttacttt agcagtttaa ttggcttaga atgatttcgg tttgcattcc
    11341 ggtttacaat ttcgatgtta ccgagctggt tggtgagctt attgctcagg caaaccggtt
    11401 gcaataccca attgagattc tcatattcga cgaccactcg cacagctact tccggaaccg
    11461 caactcgcag cttgcctcgc atagcttggt caactacctg gagttcgatt tcaacatagg
    11521 ccgttcaaaa attcgcaacc gcctagccga tgttgccacc gggcaatggt tgttattcct
    11581 cgactgcgat gtaatggttg acgacccgaa ttaccttaaa cgttacattg aaaatctacc
    11641 taacgcaaag gtggtatgtg gcggacgtaa gtatggcccc aagccgttcc gtcccgagct
    11701 tctgctccgt tggaaatacg gcgttaaacg cgaatgccgc tcggcatact cccgacagct
    11761 aaaaccatac agctcgttta tcagcggaaa cttcctgatt gagtacgaca ccttccacag
    11821 catccgattc aacgaggaac tatcaggcta cgggcacgag gacaccctcc ttggccttga
    11881 cctaaaaaag cataaggttg atatcctcca tatcgacaat ccaaccatac acatgggact
    11941 cgatagcaac caggagtttt tggaaaaaac cgatcagggc gtgaaaaatc ttgttaagat
    12001 tttgcacatt atgccctgca tgcgtaagga tattgaaaaa tcaattaccc tactcaaggt
    12061 attccgtttc ctgcgaaagg ttggaatgct ctatccctta aggtgggtgt cgcgtgtgta
    12121 cactcccctg ctccgcaaac agctatgcgg tagccgtcca tcaatcctgc tgatggactt
    12181 gtacaagcta acactactgg caaagttgta tacaaaaggc tggcgtaacc aaagccagcc
    12241 ctgagaaatg ctacttctcc ctaaagtata tctgaatagg tactccggta aggttaaact
    12301 tttcccttaa cctattttca atgaatcgct tgtaaggatc ctttatatac tgcggcaagt
    12361 tgcagaaaaa tgcaaactgt ggagttttgg tgggtaactg cgtggcatac ttaattttca
    12421 caagtttacc cttataaacc ggcggctcct tgatttgcag tgtttcctgc agaaaattgt
    12481 ttagctccga agtggaaatg cggcgcttac gattttcgta aacctgcatg gcggcttcaa
    12541 tcaccttgaa aacacgctgc ttggttaatg ccgatgcaaa aatgatggga acatcgttga
    12601 aaggggcaat tcgttccttg atgagcttgg tgtactcatc aactgttttg ttgttctttt
    12661 ccaccaaatc ccatttgttt acaaccagca ctaaaccttt cttattgcgg actataaggc
    12721 tgaagatatt catgtcctgc cgttcaattc cttgggtagc gtcaatgagt aaaaggcata
    12781 catcggagct ctcaattgcc cttacggaac gcatcacgga gtaaaactcc aaatcctcct
    12841 ttaccttagt tttacgtcgt aggccagcag tatcgacaat aataaaatcg tggccaaact
    12901 tacggtagtg ggtatcaatg gcatcgcgcg tagtgccggc caacggggtt acaatgttac
    12961 gttcctcacc aataaaggtg ttgattaacg acgatttacc cacatttgga cggccaacaa
    13021 cggctatgcg gggcacttcg ttctgctcag ctgactcatc aatatcggtg aaatgtttca
    13081 ctatatcatc gagcaaatcg ccggtaccgg aaccattgat ggatgagata caccatgggt
    13141 cgccaagacc caagctgtag aactcacccg cttgcattcg tatggcgtta ttgtcaacct
    13201 tgttggccac caggtaaacg ggctttgagc tccgcctcag tatctcggct acagcaatat
    13261 ccatgtcggt tatgccctcc tgggcatcaa ccaggaaggt gataatatcg gcttcatcaa
    13321 tagcaataag cacctgcttg cgtatctcct cctcaaaaat atcatcggaa ttttccaccc
    13381 aaccgcccgt gtcaatcact gagaattcct ttccattcca atcgcttttc ccgtaaatac
    13441 ggtcgcgggt aacgccggca atttcgtcaa caatggcatg tcgccagcca accaaacggt
    13501 taaagaatgt cgatttaccc acgttgggcc ttcctacaat agcaactaaa tttcccattg
    13561 ccctaaatat cgttttaaat gaaatactac caatatcgaa ttacaagggg taaccaaagc
    13621 gtttgagctt gccctcgtcg ttgcgccagt tgggatcaac ctttacgtaa atctccagga
    13681 atactttttt ccccagaaag gtttccagct cgaggcgtgc ctcggttccc acctttttta
    13741 gcattcgtcc ctggcttcca ataataattg acttctgcga ctccctgtcc acatagatta
    13801 tcgccctaat acggtcaatt gtttccgatt ccttgtactc ctcaatttca acctcaaccg
    13861 agtatgggat ttcctttttg tagttcagca ggattttttc ccggataatt tcggctgcaa
    13921 aaaagcggag tgtcttgtcg gttaaggtat ccttggggaa atactcaggc ccttcgggca
    13981 gcctctcaac aatcatgttt accaacgcat cggtgttaaa gccgtgcagg gctgatattg
    14041 gaagaatatc ggccttgggc atcatttgcc cccactgctc aattaggagc gaaacctttt
    14101 cctgtgtgga caggtcaacc ttgttgattg ccaggataat tggaatatcc aactgctgga
    14161 ttttgtccag gaattcctga ttcttattgg gtgtttcaac cgtgtcggta atgtaaagga
    14221 ttacatcagc atcctccagc gccgattcca caaaccgcat catagccgat tgcagcttgt
    14281 aggcaggctt cagaatgccc ggcgtatcgg agtaaacaat ctgaaaatcg tcggagttaa
    14341 caatgcccat tatgcggtgg cgggtggttt gcgccttgct ggtaacaatg gaaatccgct
    14401 cgcccacaag ggcattcatt agggttgatt tacccacatt ggggttacca ataatattta
    14461 caaaacccga tttgtgcgac atggccaaag atgattaaat cctaataaaa cctattcatc
    14521 ttaagcatgt taacctcttt ctcggtaagg aagcgccact tgccgcgtgg caaattcttc
    14581 ttggttaggc cggcaaagta aaccctatcg agctttatta ccttgtagcc aagtgactca
    14641 aagatacgac gcacaacgcg gttttggccg ctatggattt cgatacccac ctcggagcca
    14701 tcgcttccat caacgtaatc aatggcatct acctgaattt tttcgccgtc gagctcaacc
    14761 ccctcaaata ttttgttcat atcttccgga cgaacattgc ggtcgagtcc cacatggtaa
    14821 attttacgct ttttaaagct ggggtgtgtc agcctggtgg tgaggtcacc atcgttggtg
    14881 agcagaagca cgccagtgct gttgcggtcc agcctaccta cggggtaaac acgaacatcg
    14941 caggccccct caatgagctc catcacggta tgcttggcat ggggatcgtc aacggtggta
    15001 acgtaatcct tgggtttgtt aagcaggatg taaaccttgc gctcagcggt tagccgcttg
    15061 cccttataca taaccacatc atcgcggcga accttggtac ccagttcggt aaccaccttg
    15121 ccgttaacgg taaccatgcc gttagcaatc agctcatcgg cctcgcgacg gctgcacacg
    15181 cctgagttgg ctatatacct gttaaggcgg ataagctcat cggctgtttt tttggcaacc
    15241 atgccctccg cctttgcgtt atcgcgggtg tactccttgc ttttgctttc gttgtatcgg
    15301 gtatgtttta ccagcttaaa gccatcgttt ttccgtccac tttcgtgctt acgctcatcc
    15361 cgtgatggtc ttgagctacg ctcgtgtgag gtttcgcgcg gctcatgctc cctggaatcg
    15421 gaatcctttt tccatgagcg tgggcggtac gattttggct gttcggaatt gtcggatttt
    15481 ggtttgcgga aatcgcctcg gcggctatcc tctgacctgc gctttggttc cggtttgtgt
    15541 tcatcggaac ggggtgatac tcttttctcg ggcttagaat cgctttggtc gttggcatct
    15601 acctttttca acgtaagggt tctaggcctg taaaccctgg cgggctcctc gggggagtta
    15661 ctccgcttct cgcgtttaac atccctgttc atactctctt ttctcattta tatgcttaat
    15721 tattaactat ttacggctaa cgtcatagcc atttaggctt gcaaagatac aaacaaaaat
    15781 tgatttacat gctgattatt tgtgtttagt gtttggtgtt tagtttttag tgtttagtgt
    15841 ttagtgtttg gttaatataa cctgcgtaaa tcaattaaat cagcgtcatc tgcgttctat
    15901 taatctcaat ttcaatcatt aacgaacaac ttcaggtgtt aggtaaaatg gtatgcaaaa
    15961 atttaaaaag ttcgtttgat tatttgtaat aattttttat acttgctaat aaaataagac
    16021 gctacattat agcgccaatt catccaaaaa tggaaataag gcgcaactaa gtagcgcata
    16081 taaactagtt gtgtgtaatt ctaaagggca tttaaaatcc ttgaatgaat atgacaagtc
    16141 aatcaatcgc aaaactggta agtttgaaaa agattataag gaagttttaa tcctgacttt
    16201 tttgtttttc tgtggataat gcagtaaatt tcaaataatc ttaatctgct ttaaaggcct
    16261 agtatgatgg atattaattt aaactaatca taaaactatg aacagggtag taaaaatgaa
    16321 acggatattc acctacttag cattgttaat tttaattttt ataaatggta ggtgcacaaa
    16381 agataacgag tccccattac cagtaagtta tggcatttta ttttacagcg accgtgatgg
    16441 cgataatgaa atttactcga tgaacgctga tggtggtggg ttaaaaaagt taaccgataa
    16501 tggttacaat gactgcaatc cccaatggtc gcccgatggg agtaaaattg tatttgaaag
    16561 caaccgtgat ggaaataggg agatatatgt tatgaatgcc gatggaagca accagattag
    16621 gcttacatat tacaatctaa acgactggtt gccacaatgg tctcctgatg gcaaacagat
    16681 cctttttatt agggaccttg gttttggaaa ctatgagatt tttaccatgg atgccgatgg
    16741 gaataacatt acaaatctta caaatatcgc tttacaggat tacaaccccc actggtcgcc
    16801 cgacggtagc aaaattgcgt ttgaacgtaa gatcaacaat acaatggaaa ttttcgtaat
    16861 gaacaaagac gggagtaatc agatcagatt gacagacaat agcgctaatg attacaatcc
    16921 cagatggtcc cctaacggag ataaaattat tttcagtagt aacagggatg ggaacggtga
    16981 aatttatgtg atgaatgcaa acggtagtaa tcagacccgg attacagaaa acaatggcga
    17041 agactcatgg cctcaatggt ctcctgacgg atcaaaatta attgccgcaa ggtatacaag
    17101 cggaaacctt gaagtatacc ttatgaattt ggacggaaca ggatacctac gactttttgg
    17161 cacactttcc gatattgaat acccacaatg gcatcatgac ggaaacttta ttattcttgc
    17221 agcaaggaag tataacgatt gggagattta taccataaat actaataaac cggaagaatt
    17281 aaatatcaca aataataccg gtaacgacca tttgtatttt taccgttgaa tactgccaat
    17341 tcttaaggtg gctgaataat gtttcggaat attggtatta tcaataaaac aaacagaatt
    17401 ctgccactaa aattaatcga ttggttcaga tttaattcat gaattatgaa aatagaacta
    17461 cacacaacat cgtgtataaa acattggggc ttaagtggtt atattaagcg ctctgccccg
    17521 catcagtatt tgtttccgtg gataaaaatg aagcccgcaa tccccaacgt ttcataccgc
    17581 caaccgttgg gcgtaatatc ttgaaatttg agaaatttgt tgaattataa aagaaggaat
    17641 attcataaag tcataccaag caattggata attatatcta tgctattagt tataattatt
    17701 gcagtttgtt atatgtatga aaaagttcga gagtctaaat attctgttgg tggagattgt
    17761 gtttatattg aaaaagaaat ttgtccgaaa tttagtttat tatttattga ctcttcgtgc
    17821 attacaatta aagaaataaa acttgacaat aaaacaatat tcaaattgga tactactaat
    17881 aaattgaaac aatttaacca caattttata tctggaaaga aaaaaattga attgattata
    17941 aaggacaaaa gtgtttcatc agattcagtt tgtttagatg attggtgtga atactactgg
    18001 ttgacaattg aaaaggataa agaaaataat atctgtaaaa taaatattaa accagtgaca
    18061 ttagattaag tcgagtaggc aagggggaat cacaccccca agccccccgt tcggctgagg
    18121 ctatacgcct cagtcgaatc tatctattcg ggcact
//
LOCUS       DAOOWJ010000054        17818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3198_length_17818_cov_7.911727, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000054 DAOOWJ010000000
VERSION     DAOOWJ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 17818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17818
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3198_length_17818_cov_7.911727"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(568..2703)
                     /gene="scpA"
                     /locus_tag="PLW22_06775"
     CDS             complement(568..2703)
                     /gene="scpA"
                     /locus_tag="PLW22_06775"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOU74445.1"
                     /translation="MKPDFKKIDIKHIDKSSGVLNVEADWLTPERIPVKPAFTETDLE
                     NMEHLNYAAGIPPFLRGPYSTMYVMKPWTVRQYAGFSTAEESNAFYRRNLAAGQMGLS
                     VAFDLATHRGYDSDHERVVGDVGKAGVAIDSVEDMKILFDGIPLNKMSVSMTMNGAVL
                     PVMAFYIVAALEQGAKLEELSGTIQNDILKEFMVRNTYIYPPEFSMQIIADIFEYTSR
                     KMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGVKAGIDIDAFAPRLSFFW
                     AVGMNHFMEIAKMRAARLLWAKIVKQFNPKNPKSMALRTHSQTSGWSLTEQDPFNNVA
                     RTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQLYIQEETNVCRAVDPWA
                     GSYYVEYLTHEIAHKAWKLIQEVEELGGMAKAIETGIPKMKIEEAAARKQARIDSGKD
                     IIVGVNKYRLDKEDPIEILDVDNTAVREAQIRRLQKLRAERNEAEVQAALNAITRCVE
                     TGEGNLLELSVEAAKKRATLGEISYACEKVVGRYKAVIRSISGVYSAESMDDPNYQKA
                     RELCEKFAKLEGRQPRIMIAKMGQDGHDRGAKVVSTGYADIGFDVDIGPLFQTPAEAA
                     KQAVENDVHVLGVSSLAAGHKTLVPQVIEELKKLGRDDIMVIVGGVIPPQDYQFLYDH
                     GVVGIFGPGTSVASAAVKILEILLEKFEE"
     gene            complement(2736..4619)
                     /locus_tag="PLW22_06780"
     CDS             complement(2736..4619)
                     /locus_tag="PLW22_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOU74446.1"
                     /translation="MAEKSNEKLFTEFPPVSTAEWEAVIREDLKGADYDKKLVWKTLE
                     GFNVRPYYRSEDLANLETVHVKPGDFPFVRGNHQKGNPWLIRQDFEVCLDKPTEANRK
                     AIDMLSRGVESLGFNLCSDCEPSYDSISRLLKDIDLSRVEVNFTGGSASAKALPFIIK
                     FFEQSGVKPTDIKGSIDYSPLTTFALKGKFCCENDASYQGLVNVLNAVKSFPNFRVIG
                     VNAHVFHNSGSSTVQELAFGLAMASDYLNALTDKGFGIDELAARIRFNFAVGANYFME
                     MAKFRAARMLWAKMVEAYNPKSKDAARMSIHAETSRWNLTVYDPYVNMLRTTTESMSA
                     VLAGVDSLNVLPFDAPFAQPSAFSERIARNQQILLREESHFGKVADPAAGSYYIENLT
                     ISIAEYAWNLFLEVDSQGGFREAFTKGFIQEKINEVAAKRDANIASRRETVLGTNQYP
                     NFTEVADLAKVKPEAVSRPKPVKSADAIAQPLVPYRGAQAFEELRYRTDSSGRRPRVF
                     MLTLGNLAMRRARAQFSCNFFAVAGFEVIDNIGFNTVDDGVKAAMDAKSDIVVICSSD
                     DEYATLAPEAFERLNGKAIFVVAGEPACKPDLEAKGIANYISVKSNLLDTLLKYQEML
                     GIK"
     gene            5069..5296
                     /locus_tag="PLW22_06785"
     CDS             5069..5296
                     /locus_tag="PLW22_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74447.1"
                     /translation="MRRILKFVFVLASLILLIPAKSEAKFIGAECETVTTGGGDSRYV
                     TKSICKKYFFWIRYDTVVTVESIDCSHLNNQ"
     gene            5367..6011
                     /locus_tag="PLW22_06790"
     CDS             5367..6011
                     /locus_tag="PLW22_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74448.1"
                     /translation="MNYKVIVSLILLFFNSAIFAQENHFLFDSVIQIQEVTVYAKQKM
                     EVLNNKKSRNVMHIRGYGNASIVSKINLNKDNVYHIKAVEFFFNYKWSGVDNEGFYIK
                     PIIKCVDNGKPSNDLLNSQCVYFVGNNINEEIYINLSHYNIVLENTDSFFVGFELVSA
                     SENAKVEEFNITMVPVKNSQNISYLKGSCSKCDFSALNLDEKNALQLKYKIYYK"
     gene            6258..7196
                     /gene="amrB"
                     /locus_tag="PLW22_06795"
     CDS             6258..7196
                     /gene="amrB"
                     /locus_tag="PLW22_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU74449.1"
                     /translation="MSRIMSLQGEMLAKSPTVNGCRLAVCPHDDYTYVGWLYPATLRN
                     ISAKTIILFGVAHKARKFNLEDALVFDSFTHWHGAYGNIPVSELREHIIKELPGDMAI
                     RHDSMHIVEHSVESMLPFLQYSKRDIEIVPILVPCMSFDRMKEVATALATALQKVFQK
                     YDLSWGSDVALVVTTDAVHYGDEEWGGRNYAPFGTDSAGYRKAVDLELEIINTTLKGT
                     LNDVKIRNFFGYTVDAENYREYKWTWCGRYSIPFALLTAIKLNQLMKGSDLIGLPVGY
                     ATSIDHQPLPVADLGMGQTAPANIRHWVGYAAVVYP"
     gene            7809..12749
                     /locus_tag="PLW22_06800"
     CDS             7809..12749
                     /locus_tag="PLW22_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOU74450.1"
                     /translation="MELQEIDLKSYRIKFFGIKRTTRSGVVHVGWNSENIGGSSGGTA
                     QFEVPAIDDFSVLSAKVVQSPSQYIQILFSENLSENQDYEGLVDVDGLSNLKFEQDGN
                     ILRVYLPLEKQGASEVKVFKGIKNFKGKKLEHDYTERFVFEDIKPGLRSLGKGTVLPT
                     SQGLIYPFEAVNLKEVTVEVLKIFENNIPYYLQVNRLGENYDLRRVGYSIFKKNISLF
                     DYGVVVPNKWTRFTLDMSKFFEADPGALYQIKISFNKKQLLTPCDKEENSNEATPDDN
                     VTSGNDYYEDYDEYYYSDDYDWNERDNPCNSAYYSSSRMISQIILSSDIGLLAKKGEN
                     DKILVVVTDLPTAKPKSGVTVKVTDYQNQLIVEGQTNSDGIAELQLSRTPYLVTAIDG
                     NQKGYLRVDNGTSNSLSNFDVGGFEVQRGIKGMIYGERGVWRPGDTLHITFILEDKAK
                     SIPENHPIIFELHDPKGLIVKKMVASNSPVGIFYFPVPTDEQAPTGNWRAIVKVGGAV
                     FEKYLKVETIKPNRLKINFELGEIPASGEVNAPIHVKWLHGENAANLEAKYEITLSKG
                     NAVIPKYSQYNFNDPGIDFETNTYTLWEGTLDANGNSRVVGRINKSKSMPAAVNVAFK
                     GRVFEQGGDFSIDLFSKTYYPYNKLVGVLAPKPKPGMGWLETDADQEFSVVTIDAKGN
                     AVNSSQLEVELYKLSWSWWWEQLDNSDANYVSRNYDKLITRKKISTVNGKATFPLRVD
                     YPEWGRFYLKITDKETGVSCGNIVYFDWPWGYSKTQADRPGGASILAIATSKNECSVG
                     ESVQLTIPGAENARALVSIENGTRVISAWWVDASKPTNVIEVKTTPEMSPNVYLHVTL
                     IQPHKGKTNDLPIRLYGISRLVVTDPQTILQPVIQMPDEIKTNQTFDIKVSEKNGKRM
                     SFTLAVVDEGLLDINRFKTPDPHAVFYTQEAIGVKTWDIFNDVIGAYGGKLERHISIG
                     GDDELRSQDGDNNQRFKPVVRYFGPFSLGKNDTKKVSVNLGNYIGSVRVMVVAAYEGA
                     YGNAEKACPVRNDLMVLATLPRVLGPQEEISLPVNIFTMTDKVKNVTVKVKTSSNISI
                     VGDEQRSVSFNKPGDRMVYFNLRVDGTGPAKVTVEASGNEVKSYQTIDIYVRNPISAV
                     TRSIDTIITANGKISALFSTFGIPGSNSAMLEASVMPPLNLKRRLDELIQYPHGCIEQ
                     TTSAVFPQIYLDKLVELSSEQRGNINYFVNESITKLSKLQNTDGSFRYWPSYNYSDEW
                     STSYVGHFLIEAQNAGYNIPQSILNNWVNYQRNAAREYLYDASRYTNSGLIQAYRLYT
                     LALAKKPEIGAMNKLKEQQLDLTAKMRLAAAYALIGQVETAKALIQNTPIKFEPYREL
                     SFTYGSDLRDKAIVLETLVLLNDDSNGFRLAREISNSLSGNRWLSTQETAVSLLALSK
                     MSMQTKERSDVNLRYTINGKTEKITTEKPIARSVLQIKEGNNNITYENLSTKPMFVNL
                     SLTGIPPLGEEKTFESNITMKVAYLSNDGNVISPEQLKKGTDFKCEVTVFNPGVRGEV
                     KELALTQIFPSGWEIQNTRLESGENTTSSNFDYQDIRDDRVITYFSLKPNESKKFSVK
                     LTATYAGKFYMPGPYCEAMYDNTIASYKKGGWVRVE"
     gene            12697..15105
                     /gene="pbpC"
                     /locus_tag="PLW22_06805"
     CDS             12697..15105
                     /gene="pbpC"
                     /locus_tag="PLW22_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOU74451.1"
                     /translation="MITLLHRIKKAAGLEWNKLIKPLVIIKTAKPFIKYMLAGVILLL
                     AFLLFVKPVSFKASSSTVLLDRNGQLLAATVTDEGMWQFPAPDSVPFRFSKCLLQFED
                     SYFYYHPGFNPVSLVRAAIQNLRNRKIISGASTITMQLVRLSRKGKQRTYMEKAIEIV
                     LAFRTELAYSKKSILAMYASHAPFGGNVIGLDAAAWRYFGTSPDNLSWAEAATLAVLP
                     NAPGLIFPGRNQHLLLAKRNFLLGKLLKKGVIDNQTYQLALLEPLPDPDNKYSQRATH
                     LLTRCIKDGLKGKIIRSTISRELQDIFYKIALRYHRHFKANGIRNMAILVVDNLTGEV
                     LVYIGNILTDDPSFSSKVDIITAKRSYGSLLKPFLYAYMLSEGLLLPKEFVKDVPVAI
                     AGFTPLNFSKKFEGAVKANEIISRSLNVPSVILLKEYGIEKFYNNIHKTGLLSINMPP
                     SHYGLSIILGGAESTMWELARAYSAIARAALSDTLRETSIRYTVDRGMLFNQAFEAAN
                     IKPGAAYLTLQALTEANRPGEDGTWRNFIASQTIAWKTGTSFGFRDAWAIGVTPKFTV
                     SVWVGNADGEGRPDLVGAATAAPVMFDVFSVLPSCSWYSPPYYDLKTVKTCKKTGMLA
                     SNLCPDTTEELVPAVKIKTLPCIYHKKIFLDKSKRYRVNRSCFPNSDLKDTTWFILPP
                     GQEWFYKKNHFDYTVLPPMHPKCSVQYDEINNIDLIYPFYGVDLYIPIENDGTRGQII
                     LKATHRKSESIIYWHLNNEFIGMTKNVHEIAISPKPGSYTLTLVDENGEELKRRIKII
                     GEDR"
     gene            complement(15312..16538)
                     /locus_tag="PLW22_06810"
     CDS             complement(15312..16538)
                     /locus_tag="PLW22_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU74452.1"
                     /translation="MNVPLYIARRYLFAKKSHNIINIISVISVIGVATGVMALVVVLS
                     VFNGFDSLIANLYSHIDADLRIEAKQGKTFRTDSLPIERVKQIPGVAVFSEVVEENAL
                     FRYRGKQHIGIVKGVSRNYTELTGLKSKIVDGDFKLWQGSQPMAIMGEGVAYYLNANL
                     AHFDPLFVYIPRRGKNYSPDAAFIKKAIMPSGIFAIEQDFDTRYVITPVEFARGLLLY
                     DSLTVSAIEIKLHTSANAARVKTDVENIIGNKYRVLDRYQQNESLYRTMKSEKFAIAL
                     ILILILIIASFNIVGSLSMLIIDKRADVETLKSLGASNSLIQKIFLFEGMLISIGGSI
                     IGIIIGLITCWLQITFKLIRLEGRGNFIVDAYPVEVQASDMILIFLVVIAIGYLAARF
                     PVRVITRRLIQNDSRN"
     gene            complement(16544..17314)
                     /locus_tag="PLW22_06815"
     CDS             complement(16544..17314)
                     /locus_tag="PLW22_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU74453.1"
                     /translation="MISRLVSFTTRFIPRHYLQLFASFLMKAIGLFYRGSRFQDPIDG
                     VKYRKLLPYGRVTTRENALAPNSMSLERHRLIWLYLQRKTDFFTAPKKVLHVAPEYCF
                     IKPIKRLRNIDYTTADLISPWADVKLDVQAMPFADNTFDMVICNHVLEHVDNDLRAMA
                     EILRVLKPGGFAVLQVPMDLNMEQTLENPEYNTPELREKYYQQRDHLRLYGRDYAKRL
                     TSAGFDVLEDNFVKQLPPHEVEYYALPKDEILYIARKK"
     gene            complement(17325..17663)
                     /gene="rbfA"
                     /locus_tag="PLW22_06820"
     CDS             complement(17325..17663)
                     /gene="rbfA"
                     /locus_tag="PLW22_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOU74454.1"
                     /translation="MEGTRLQKVGRLIQKELSEIFQREGAARFPGKMLTVTQVRVSPD
                     LSVAKVYVSIFPSTDTDKTLEHIRDNSKFLRGELGKRVRHQLRVIPELTFFIDDSLDY
                     IEKIDQLLKE"
BASE COUNT         5272 a         3514 c         3896 g         5136 t
ORIGIN      
        1 tacaattcgc cacgccttcg gcgtgactca tgtggtatcg cttactaagc ccaccaccac
       61 gctccacccc cgacccattt tcgggccagg cttttatgcc actgcttaag tcattctttg
      121 ttggttgagg aatttcatta ataaagggaa tgaaggtaca aagggatgct tcggcgagct
      181 cagcatgaca atgggagtaa ggaaaaaagg ggaatgtcat tccgaactag tagaggaatc
      241 tctctcaggt atagcgaaca gccctgtagg ggcaaatcaa ctaagcccag ggttttaacc
      301 ctgggtattg ttgtattcaa aattttgggc gatgcacgca ataccttttc caaagaacaa
      361 ccctgaaacg ttgcatcgca acaaaaaaca ttgcaaagtg caattgcagg aaaacaattc
      421 cccaaggata caggacttaa ggtatataga gatgcttcag cttgctcagc atgacagaaa
      481 ggcgttttag cggagtatat acttataaat caggaataaa taaaaaaggg ttgcaatcgc
      541 aacccttttt cagtttaaac gatgtgatta ctcttcgaat ttttcgagca ggatttccag
      601 aattttaacg gcagctgaag ccactgaggt tccggggcca aatatgccca caacgccatg
      661 gtcgtaaagg aactggtagt cctgaggagg aattacgccc ccaacaataa ccatgatatc
      721 gtcgcggcca agtttcttta attcctcaat tacctgagga actagggttt tgtggccggc
      781 ggccaagctg gacacgccaa gcacatgaac atcgttttca acggcttgtt tagctgcctc
      841 agcaggggtt tggaatagag gcccaatgtc cacatcaaag cctatatcgg catagccggt
      901 cgatacaacc ttggcgccac ggtcgtggcc atcctgcccc attttggcaa tcatgatacg
      961 gggctggcgt ccctcgagct tagcgaactt ttcgcagagc tcacgggctt tctggtagtt
     1021 aggatcatcc atagattctg ctgagtaaac gcctgaaatg gaacggataa ccgctttgta
     1081 acggccaacc actttctcgc aggcatatga tatttcaccg agtgtagcac gtttcttggc
     1141 agcctcaacc gatagttcca gcaggttccc ttcgccggtt tctacgcaac gtgtaatggc
     1201 attgagggca gcttgaacct ctgcctcgtt gcgttcagca cggagttttt gcaggcgacg
     1261 gatctgcgct tcgcgcacgg cggtgttgtc aacatcaagt atttcaatgg gatcttcctt
     1321 gtccaggcgg tacttgttaa caccaacaat gatatccttg cccgagtcaa tacgtgcttg
     1381 cttgcgggct gcggcttcct ctattttcat ttttggaatg ccggtctcaa tggcttttgc
     1441 cattccgcca agctcttcta cctcctgaat aagtttccat gccttatggg ctatctcgtg
     1501 ggtaaggtac tcaacgtagt acgaacctgc ccatgggtca actgcgcggc aaacgttggt
     1561 ttcctcctga atgtatagct gagtgttgcg ggcaatacga gccgagaaat cggttggaag
     1621 ggcaatggcc tcatcgagtg cgttggtgtg caagctttgt gtatgaccca gcgctgctgc
     1681 cattgcctca atgcaggtac gggctacgtt gttaaaggga tcctgctcgg ttaagctcca
     1741 ccccgatgtt tggctgtgtg tacgcagcgc catcgatttg ggattcttgg ggttgaactg
     1801 cttaacaatc ttagcccaga gcaagcgtgc agcacgcatc ttggctatct ccatgaagtg
     1861 gttcatgcca acagcccaga aaaacgatag gcggggcgca aaggcatcta tatcaatacc
     1921 agcttttacc ccagtgcgca ggtactcaag cccgtcggca agagtgtatg ctagctcaat
     1981 atcggcagta gcaccggcct cctgcatgtg ataccctgaa atggagatgc tgttgaactt
     2041 gggcatcttc cttgaggtat actcaaagat atcggcaata atctgcatgg aaaattcagg
     2101 agggtaaatg taggtgttac gcaccatgaa ctccttcagg atgtcgttct gaatggttcc
     2161 gcttagctcc tcgagcttgg caccctgctc aagcgcagcc acaatgtaga atgccataac
     2221 ggggagtact gcgccgttca tggtcattga aaccgacatc ttgttgaggg gaatgccgtc
     2281 gaaaaggatt ttcatatcct ctaccgagtc gatagccacg cctgccttac ccacgtcgcc
     2341 aactacacgc tcgtggtcgg agtcgtagcc gcggtgagtg gctaagtcga atgctactga
     2401 aagccccatc tggccagcgg ccaggttgcg gcggtaaaag gcatttgatt cctcagcggt
     2461 tgaaaagcct gcatactgtc ttacagtcca aggtttcatc acgtacatgg tgctgtaagg
     2521 gccgcgaagg aaaggcggaa tacctgccgc atagttcagg tgctccatgt tctccaggtc
     2581 ggtttcggta aaggcaggtt tcaccggaat acgttcgggt gttaaccagt cggcctctac
     2641 atttaacaca ccgctacttt tatcgatgtg ttttatatct atctttttaa aatcgggttt
     2701 cattgtttac ctcttatggt taagtaaaat tgcagttact taattcccag catttcctga
     2761 tacttgagca gggtatcgag caggttgctc ttcacgctga tgtagttggc aatccccttt
     2821 gcctcaaggt cgggtttgca ggcgggctcg ccggccacaa caaaaattgc tttgccatta
     2881 agcctttcaa aggcttcggg ggcaagtgtg gcgtactcat cgtcggagct gcaaatcacc
     2941 acaatgtcgg acttagcatc cattgcagcc tttacaccat cgtcaacagt attgaaaccg
     3001 atgttgtcaa taacctcaaa gccagcaacg gcaaagaagt tacagctgaa ctgggcacgt
     3061 gcacgacgca tggccaaatt gccaagggtt agcatgaaca cacgtgggcg acggccgcta
     3121 ctatcggttc ggtagcgcaa ctcctcaaaa gcctgagcac cacggtaagg aacaagaggt
     3181 tgagcaatag catcggctga tttcacgggc tttggacggc taactgcctc gggtttaacc
     3241 ttggcaaggt cggcaacctc ggtaaagttg gggtactggt tggttccaag aacggtctcg
     3301 cggcgcgagg caatattggc gtcgcgttta gcagcaacct cattaatctt ttcctgaata
     3361 aatccttttg taaaggcttc gcggaatcct ccctggctgt caacctcaag gaataggttc
     3421 caggcgtact cagcaattga gattgtaagg ttctcaatgt agtagctgcc tgcagcagga
     3481 tcggctactt tgccaaagtg cgactcctcg cgaagcaata tttgctggtt gcgggcaata
     3541 cgctcactga aagccgaagg ctgagcaaat ggtgcatcga atggcaacac gttgagcgag
     3601 tcaactccag ccaatacggc gctcatgctc tcggttgtgg tgcggagcat gttaacgtat
     3661 gggtcgtaaa ctgtaaggtt ccaacggctg gtctcggcat gaatgctcat gcgggcagca
     3721 tccttgctct tggggttgta ggcttcaacc atttttgccc agagcatgcg tgcagcacgg
     3781 aacttagcca tttccatgaa gtagtttgct cccactgcaa agttgaaacg gatacgagct
     3841 gctaactcat cgataccaaa acccttgtcg gttagggcgt ttaggtaatc gctggccatg
     3901 gcaaggccaa aggccaactc ctgaacagtc gaggagccac tgttgtggaa tacatgggcg
     3961 ttaacaccaa taaccctgaa gtttgggaaa ctctttacgg cgtttagtac attcaccaaa
     4021 ccctggtacg aggcatcgtt ctcgcagcag aatttgcctt taagcgcaaa agttgtgagc
     4081 gggctgtaat caatagaacc tttgatatcg gttggcttta cgcccgactg ttcaaaaaac
     4141 ttgataatga aaggaagcgc cttggctgat gctgatcccc ctgtaaagtt aacctccacc
     4201 cttgaaaggt cgatatcctt aagcaatcgg ctaatggagt cgtatgaggg ttcacagtcg
     4261 ctacataggt taaaacctaa cgattccaca ccgcggctca gcatgtcgat cgcttttcga
     4321 ttggcttcgg tgggtttgtc gaggcatacc tcaaaatcct gacggatgag ccaaggatta
     4381 cctttttgat ggttacccct tacaaacggg aaatcgcctg gtttaacatg aactgtttca
     4441 aggtttgcaa gatcctcgga gcggtagtat ggcctaacgt tgaacccctc gagcgttttc
     4501 cataccaact ttttatcgta gtcagctcct ttcaaatcct cgcggattac cgcttcccac
     4561 tcagctgttg aaaccggcgg aaattcggta aaaagcttct cgttgctttt ttctgccata
     4621 atcagacttt taggattttt gatttgcaaa tttaacaatt gctacaatag cagcctgtta
     4681 atttggggtg ataattaagc gaataatgct attaagcata aaaattggtt gcggtagggt
     4741 taaacaggct taacactgat ttgttatttt aaagagttta agtacgtctt atgatgcttt
     4801 tagttattga atagtcattt taaggtatca aagattctca ctaatgcaat cctaattgac
     4861 catataatga agaaatcgct taggatgcgt ataataatat atgttaatat tacgaattgt
     4921 gttgaaaaat taaaatatgc tgtataacat aatataaaat agattaatct attattatta
     4981 ttattgtaga tgtttggatt taggcctaat caaaaactac ccaaaaccca agggtatatt
     5041 tgtttgggtt tattaaatat taaactatat gaggagaata ttaaaatttg tatttgtatt
     5101 ggcttcatta atactactta ttccagctaa atcggaagca aagtttattg gagcagagtg
     5161 tgaaactgta accactggtg gaggtgatag tcgctacgtt actaaaagta tttgtaaaaa
     5221 atactttttt tggatccgtt atgatactgt tgtaactgtt gaaagtattg actgctcaca
     5281 tctaaataat cagtaatttt ttacgactgg aataatgttc atattatacc agtcattttt
     5341 tgtttacata ttaaaaaaat actataatga attacaaagt tatcgtatct ttaatactat
     5401 tattttttaa ttctgccatt tttgctcaag aaaatcattt tctatttgac tcagtgattc
     5461 aaatacaaga ggttacggtt tatgccaaac aaaaaatgga agttctaaat aataaaaaaa
     5521 gtagaaacgt aatgcatata agaggctatg gtaatgcttc aattgtatcc aaaataaatt
     5581 taaataaaga taatgtatat cacatcaaag cagttgaatt cttttttaat tacaagtgga
     5641 gcggcgtgga taatgagggg ttttatatta aaccaataat taagtgtgtt gataatggta
     5701 agcctagtaa tgatttgtta aactcacaat gtgtttattt tgtaggtaat aatattaacg
     5761 aagaaatata tattaattta tcgcattaca atatcgtatt ggaaaatact gattcttttt
     5821 ttgtgggatt tgagttagtt agtgcttcag aaaacgctaa ggttgaagag tttaacatca
     5881 ctatggttcc tgtaaaaaac tctcagaata tatcatatct taaaggttca tgttctaaat
     5941 gtgatttttc agcacttaat ttggatgaga aaaatgcgct ccaactaaag tataaaatat
     6001 attacaagta gtttatattc aaaaaatttt accaaaaact tattttacca acatttatct
     6061 aatattaaat aattgattaa ttgataaaat atttatattt ttaggcatca acataggata
     6121 tagtaatgaa ctttaggtta atagtgctat tgtcactctt tttgacaaat tgtaagcacg
     6181 aatataagaa tgtatcggtt cgcaagccag tcgatactat tggatttgct acaaagcagt
     6241 atcaaataga ttcggtcatg agccgaatta tgtcactaca gggcgaaatg cttgcaaagt
     6301 cccctacagt taatggctgt aggcttgcag tttgcccaca cgacgactat acctatgtgg
     6361 gatggctata cccggccacc ctcaggaaca tcagcgcaaa aactattatt ttatttggtg
     6421 tggcacacaa agcccgcaag tttaaccttg aggatgcgct agttttcgat tccttcacac
     6481 attggcatgg tgcttacgga aacattcctg tgtcggagct gcgtgaacac atcatcaaag
     6541 aattgccagg ggatatggct attaggcacg atagcatgca tattgttgaa cactccgttg
     6601 agtcgatgct acctttcctt cagtatagta aacgcgatat cgaaattgtg ccaatcctgg
     6661 ttccatgcat gagctttgat aggatgaagg aagtagcaac agcgcttgca actgcccttc
     6721 aaaaggtttt tcagaagtat gatttgagtt ggggttccga tgttgctttg gtagtaacga
     6781 ccgatgcagt acattacggt gatgaggagt ggggtggcag gaattatgcc ccctttggta
     6841 ccgattcagc aggttacaga aaggcggttg atctggaact cgaaattatt aataccacac
     6901 tgaaagggac actcaacgat gtgaaaatcc gtaacttttt cggttatacg gtcgatgcag
     6961 agaattacag ggaatacaaa tggacctggt gcggccggta tagtattcca tttgcgttgc
     7021 ttactgccat taagctgaat cagcttatga aaggatccga cctcattgga ttacctgttg
     7081 gttacgctac cagtatcgac caccaaccct taccggtagc agatctgggt atgggtcaaa
     7141 ccgcaccagc gaatatcagg cactgggtgg gctatgcagc ggtggtttac ccgtagtcac
     7201 aattgaatta cctctaaatt ttaaataata tgagaactag taaatttaat ttattaaaaa
     7261 tcctattact cgttttaata ggtgtttcaa cattgatttc atgtaagaag aaagcaaaag
     7321 acatcataat cgaagttgat ccaacttttg gccaatacat ttcggcatat acatcgggca
     7381 tggtgtcagc caattcaact attacaattc gatttacaat gcctgcgcaa aaatttacta
     7441 aggcaggtga cgaattagac gataatgtat ttaaaatttc acccagcata gatgggaaaa
     7501 cctattgggt ggatgatcaa acaatcgaat tcaaacctac taatccttta gtatctggcg
     7561 aaatatatac aataaggttt tttcttggca aagtactaga agttcatgat agcaggtttg
     7621 aaaagtttga tttttcgata cgagtaattc ctcaatctat tgacgtagat tttgatggtt
     7681 taataattaa cgggaaggat gactctatta cctataatct agagggcact gtgagggtgg
     7741 cggattatac ttccattgaa aaattgacaa aatgcttttg ggcaaattat aatagtcaaa
     7801 atttagagtt ggaacttcag gaaattgatc tgaaatccta cagaatcaaa ttttttggca
     7861 taaagaggac aacccgttcg ggtgttgtac atgtaggttg gaattctgag aacattggtg
     7921 gaagttccgg tggcactgcc caatttgagg tacctgctat tgatgacttt tctgttctta
     7981 gtgcaaaagt agttcaatct cccagtcaat acattcagat tctattttcg gaaaatctga
     8041 gtgagaatca ggattatgaa ggtttagtag atgttgatgg gttgtcaaat ctgaaatttg
     8101 agcaggatgg gaatatactg agagtttatc ttccattaga aaaacagggt gctagcgaag
     8161 tgaaagtttt taaaggcatt aagaatttta aagggaaaaa gttagagcac gattacactg
     8221 agcgttttgt ttttgaagat ataaaacccg gattacggtc actgggtaaa ggaactgtac
     8281 ttccgacatc gcaaggttta atttatcctt ttgaagcggt taaccttaaa gaggtcaccg
     8341 ttgaagttct taaaattttc gaaaacaaca ttccttacta cttgcaggtc aatcgattag
     8401 gagagaatta cgatttgcga agggtgggtt acagtatttt taaaaagaat atctcattgt
     8461 ttgattatgg agttgtggtc cctaataaat ggactcgatt cacacttgac atgagcaaat
     8521 tttttgaagc ggatcctggg gctttgtatc aaattaaaat ctctttcaat aaaaagcaac
     8581 ttttaacacc atgcgacaag gaagagaatt ccaatgaagc aactcctgat gataacgtaa
     8641 cttcgggaaa tgactattac gaggattatg atgaatatta ttacagtgat gattatgact
     8701 ggaatgagcg cgataaccca tgtaattctg cttactactc aagttcaaga atgataagcc
     8761 aaattatact gtcatccgat ataggtttgc tagctaaaaa gggcgagaac gataagatac
     8821 ttgttgttgt caccgacctg cctacagcaa aaccaaaatc gggtgtaact gtaaaagtta
     8881 ctgattacca aaaccagtta attgttgaag gacaaacgaa ttctgatggt attgctgaat
     8941 tacaactctc acgcacaccc tatcttgtaa ctgccataga tggaaaccaa aagggatact
     9001 taagagttga taacggaacg tcgaactcgt tgagtaactt tgatgtagga gggtttgagg
     9061 ttcagcgagg aataaaggga atgatttatg gtgagcgtgg ggtatggcgt ccaggcgaca
     9121 cccttcatat tacatttatc cttgaggata aagcaaaatc cattcctgaa aatcatccca
     9181 taatttttga acttcacgac cccaaagggt tgattgttaa aaaaatggtt gcatctaata
     9241 gccctgtagg tatcttttat ttcccagttc caaccgatga acaagcacct acaggtaatt
     9301 ggagggctat tgtaaaagtt ggtggcgctg tatttgaaaa gtatttaaag gttgaaacca
     9361 tcaagcccaa taggctgaaa ataaattttg aattgggtga aattccagcc tcaggcgaag
     9421 ttaatgcccc tattcatgta aaatggttac atggcgaaaa tgcagctaac cttgaggcca
     9481 aatatgagat aaccctttca aaggggaatg cagtaattcc aaaatatagc cagtataatt
     9541 ttaacgatcc tggtattgat tttgaaacta atacctatac tctttgggag ggcacgttag
     9601 atgccaacgg aaactctcga gttgtaggta gaatcaataa atcgaagagc atgcctgcag
     9661 ccgtaaatgt tgcattcaaa gggcgtgtat ttgaacaagg aggcgatttt agcatcgatt
     9721 tattctcaaa aacatactac ccctacaaca agcttgtggg tgttttggct ccaaaaccca
     9781 aaccaggaat ggggtggctc gaaactgatg ctgaccagga attttcagtg gttactatcg
     9841 atgcaaaagg aaatgcagtt aatagttcac aacttgaagt tgaactttac aagctttcct
     9901 ggagctggtg gtgggagcaa ctcgacaata gtgatgcaaa ttatgtaagc aggaactatg
     9961 ataagttgat taccagaaaa aaaatatcaa cagttaacgg caaggcaact ttcccacttc
    10021 gggtagatta cccagaatgg gggcgtttct atcttaaaat taccgataag gagacaggag
    10081 taagttgtgg caatattgtt tactttgatt ggccttgggg atatagtaaa actcaagccg
    10141 atagacccgg aggtgcttct atattagcta tagcaaccag taagaatgaa tgtagtgtag
    10201 gtgagagtgt gcaattgact attcccggtg cagagaacgc tcgtgcttta gtaagtattg
    10261 aaaatggcac tagagttatt tcagcttggt gggttgatgc ttcaaaacct actaatgtta
    10321 ttgaggttaa aacaacccct gaaatgtcac caaatgttta cctgcacgta acccttattc
    10381 agccccataa aggtaaaacc aacgatttac ccataagact ctacggaatt tctagacttg
    10441 ttgttaccga tccgcaaact attcttcaac ctgtaattca aatgcctgat gagataaaaa
    10501 cgaatcaaac ctttgatatt aaagtatctg aaaagaacgg gaaaagaatg agttttactc
    10561 ttgcagtggt tgatgaaggc ttgctcgata taaatcgttt taaaacacct gatccgcatg
    10621 cggtttttta tactcaggaa gcaatagggg taaaaacatg ggatattttt aacgatgtaa
    10681 ttggagccta cggaggaaag cttgaaaggc atatttccat aggcggcgac gatgaactca
    10741 ggtctcagga tggtgataat aatcagcgat tcaaacctgt tgtacgatat tttggcccct
    10801 ttagtttagg taaaaacgac actaaaaaag tttcggttaa ccttggtaac tacattggaa
    10861 gcgttagggt aatggttgta gctgcatatg agggtgcata cggaaatgcc gaaaaagcat
    10921 gtccggtaag aaacgattta atggttttgg caactcttcc aagggtatta ggaccgcaag
    10981 aggaaatcag tcttccggta aacattttca caatgacaga caaggtgaaa aatgttacgg
    11041 tcaaggtgaa aacatcgtca aacattagca ttgtaggcga tgaacaaaga tcggtaagtt
    11101 ttaacaaacc aggagataga atggtttact ttaaccttag agttgatgga actggacctg
    11161 caaaagtaac tgtggaggct tctgggaacg aggtaaagtc atatcaaact attgatattt
    11221 atgttcgaaa tcctatatcg gcagtaactc gaagcattga tacaataatt actgccaatg
    11281 ggaaaatttc agctttattc agcacatttg gtattcctgg tagtaactca gctatgctag
    11341 aagcatcggt aatgccacct ttaaacctaa agcgtagact cgatgaactt atccaatacc
    11401 cacacggctg tattgaacaa actacgtctg ctgtattccc ccagatatat ctggataaac
    11461 ttgttgagct ctcatcggag caaagaggta atataaatta ctttgtcaat gaatcgataa
    11521 ccaaactttc aaaacttcaa aatactgatg ggagttttcg ctattggcca tcgtacaact
    11581 atagtgacga gtggagcacc agctacgttg gtcattttct gattgaagca caaaatgctg
    11641 gttataatat tccgcaatcg attttaaaca attgggttaa ctatcaacga aatgctgcac
    11701 gtgaatacct atatgacgct agccgttaca ctaactctgg cttgattcaa gcttataggc
    11761 tttatacatt agctcttgct aaaaagcctg aaattggagc aatgaataag ctaaaggaac
    11821 aacagttgga cttaactgca aaaatgagac ttgctgccgc ttacgcctta attggtcagg
    11881 ttgaaacggc aaaagcactt attcaaaata cacctataaa gtttgagcct tatagggaac
    11941 ttagtttcac ctatggttcg gatttacgag ataaggcaat agttcttgaa actttagttc
    12001 tacttaatga tgattccaac ggtttccgat tagctagaga aataagtaac agtctttcag
    12061 gtaaccgttg gttaagcaca caggaaacgg cagtatcact acttgcctta tcaaagatgt
    12121 ccatgcagac taaagagaga agcgatgtaa accttcggta taccataaat ggaaaaaccg
    12181 agaaaattac taccgaaaaa ccaattgcta ggagcgtatt gcaaataaag gaaggtaata
    12241 acaatattac atatgagaat ttatctacta agccaatgtt tgtaaatctt tcacttactg
    12301 gtattcctcc tttaggtgaa gaaaaaacat tcgaaagtaa tattaccatg aaggtagcat
    12361 acctttccaa cgatggtaat gtaatcagcc cagagcaatt aaaaaaaggt accgatttta
    12421 aatgtgaggt aacagttttc aatcctggtg ttagaggaga agttaaggag ctagcactaa
    12481 ctcaaatctt cccatcgggg tgggaaattc aaaatactcg attagaaagc ggcgagaata
    12541 ctacgtcgag taactttgat tatcaagata ttcgtgacga tagagtaatt acatacttta
    12601 gcttaaaacc taacgaaagc aagaaatttt ctgtaaaact tactgcaacc tatgccggaa
    12661 aattttacat gcctgggccg tactgtgaag caatgtatga taacactatt gcatcgtata
    12721 aaaaaggcgg ctgggttaga gtggaataaa ctgattaagc cattggtgat tattaaaaca
    12781 gccaagcctt ttataaaata tatgcttgca ggagtaattc tcctgcttgc atttttattg
    12841 tttgtaaaac cggttagttt taaggcttcg tcctcaactg ttttgcttga ccggaatgga
    12901 cagttgcttg cagccacagt aaccgatgag ggaatgtggc agttccctgc gcccgatagt
    12961 gttcccttcc gattttccaa atgtctttta caatttgagg actcatactt ttactatcat
    13021 cccggtttta atccggtttc gttagttaga gctgctattc aaaatttaag aaatcgtaaa
    13081 ataatcagtg gtgcgagtac aatcactatg caacttgttc ggctatcaag aaagggtaaa
    13141 caacgaactt acatggaaaa agccattgag atagttttag ctttccgaac agaacttgca
    13201 tactcaaaaa aaagcatttt ggccatgtat gcatcgcatg ctccttttgg gggtaacgtt
    13261 attggccttg atgccgctgc ttggcgatac tttggtacaa gtccggacaa tttgagttgg
    13321 gctgaagctg caacactcgc ggttcttcct aacgcccccg gtctaatttt cccgggcagg
    13381 aatcaacatt tactcctagc taaaaggaac tttcttctag gaaaactttt aaaaaagggg
    13441 gttatcgata accaaacata tcaactggca ctgttggagc cattgccaga tcctgataac
    13501 aaatatagcc agagagcaac ccacttgctt acacgttgta ttaaagatgg attaaagggg
    13561 aaaattattc gttctactat tagtcgtgaa cttcaggata ttttttacaa aatagccctg
    13621 cgctatcatc gtcattttaa ggcaaatggc ataagaaata tggcaatttt ggttgtggat
    13681 aatttaaccg gagaagttct agtatacatt ggtaatattt tgacagacga tccaagcttt
    13741 tcatctaaag tagatatcat aacagctaaa cgaagttatg gtagtttact taaacctttc
    13801 ctttatgcat atatgctttc cgagggtctt ttattaccta aggaatttgt taaagatgta
    13861 ccggtagcta ttgctggatt tacaccttta aattttagta aaaaatttga gggtgcggtt
    13921 aaggctaatg agataatttc tcgctcatta aatgttccct cagttattct cctgaaagaa
    13981 tatggaatcg aaaaattcta caataacatt cataagaccg gtttacttag tattaacatg
    14041 cctccttcgc attacggttt gtcaataata cttggaggtg cagagtccac tatgtgggaa
    14101 ttggctcgtg catactccgc aattgcaagg gctgcattaa gcgatacttt gcgtgaaacc
    14161 agtatccgtt atacggttga tagaggaatg ctttttaacc aagcatttga agctgctaat
    14221 attaaacccg gtgcagcgta tcttacgctt caagcattaa cagaggcaaa tagacccggg
    14281 gaggatggca catggcgtaa ttttattgct tcgcaaacta tagcttggaa aaccggaaca
    14341 agttttggtt tcagggatgc ttgggcaatt ggtgtaaccc caaaatttac agtttcagtt
    14401 tgggttggaa atgccgatgg cgaaggccga cccgatttag ttggtgcagc aacagcggct
    14461 cctgtaatgt ttgatgtttt ttctgtttta ccttcgtgta gctggtattc gccaccttac
    14521 tatgatctaa aaacggtaaa aacatgcaaa aaaacgggta tgcttgcttc aaacctttgc
    14581 cctgatacta cagaagagtt ggttccagca gtaaaaatta aaacattacc atgcatttat
    14641 cacaaaaaaa tttttcttga taaatctaaa cggtatagag taaatcgttc ctgttttcca
    14701 aatagtgatt taaaagatac aacttggttc atccttcctc caggacagga gtggttttac
    14761 aaaaaaaatc attttgatta tactgttctc cccccaatgc accctaagtg tagtgttcaa
    14821 tatgatgaaa taaacaatat tgacctcata tatccatttt atggagttga tctttatatt
    14881 ccaatagaaa atgatggcac tcgtggtcaa ataattttaa aagcaacaca tagaaagtct
    14941 gaatcaatca tttactggca tttgaataat gagtttatag gtatgactaa gaatgttcat
    15001 gaaatagcca tcagtccaaa acctggaagc tatactctaa cacttgtcga tgaaaatggt
    15061 gaggaattaa agcgtagaat aaagatcatt ggagaagata ggtaatcttt caaaaaatta
    15121 ggattattta ttagttttaa ttatctggta atttgttatg gcttaatttg tttattgaat
    15181 ttattttttt tgattcaatt tcaaataata tgaacaaaca tcatcctgtt aggatgaact
    15241 tacattttgg tttaaaacaa agaaaaatca aaatggaaag tatacttgaa aaattaataa
    15301 aaaaagcgtt tctaattcct actatcgttc tgaatcaacc tccgcgttat aaccctaacc
    15361 ggaaatcggg ctgccaggta tcctatggct ataaccacca ggaaaattag gatcatatcg
    15421 ctggcctgga cttcaacggg gtatgcatca acaataaagt tgccacggcc ctcaagccta
    15481 atgagcttaa aggtgatttg cagccaacat gttatcaaac caattataat tccaattata
    15541 ctgccaccaa tggaaataag catgccctca aagagaaata tcttttgtat caggctattg
    15601 cttgctccaa gacttttcag agtctccaca tcggcgcgtt tatcaataat tagcatggag
    15661 agcgatccca ctatattgaa tgatgcaata atgagtataa gtatcagtat tagcgcaatg
    15721 gcaaactttt ccgatttcat ggttcggtaa agcgactcgt tttgctggta acggtccaat
    15781 accctatact tattgccaat aatgttttca acatcggttt taaccctggc ggcatttgca
    15841 cttgtgtgca gcttgatttc aatggcgcta acagttagtg aatcgtaaag gagtagaccc
    15901 cttgcaaact caacgggtgt aattacatac cgggtatcaa aatcttgctc aatggcaaaa
    15961 atgcccgagg gcataatggc ttttttaata aaggcagcat cgggggagta gtttttgccc
    16021 cgcctgggaa tgtaaacaaa cagcgggtca aagtgggcta gattggcgtt gaggtagtag
    16081 gcaacccctt cgcccatgat ggccatgggc tggctacctt gccatagctt gaaatcgcca
    16141 tcaactattt tgctctttag tccggttagc tcagtgtagt tcctgctaac ccctttaaca
    16201 attccaatgt gttgcttccc gcggtagcgg aaaagggcat tctcctcaac cacttcggaa
    16261 aatactgcca caccgggaat ctgcttgacc ctttcaatgg gtaagctatc ggttcggaat
    16321 gtttttccct gcttagcttc aatccgcaaa tcggcatcga tatgggagta gaggttggca
    16381 atcagggaat cgaagccatt gaaaacggat agaacaacca caagcgccat aacccctgtt
    16441 gcaaccccaa taaccgatat tacggagatg atgttgatga tgttatgcga ttttttcgca
    16501 aacaggtaac gtcgggctat gtaaagcgga acattcattg gttttacttt tttcgggcaa
    16561 tgtaaaggat ttcgtcctta ggtagggcgt aatattccac ctcgtggggt ggcagctgct
    16621 taacaaagtt gtcctctaaa acatcaaacc cggcagaggt aaggcgtttg gcgtaatcgc
    16681 ggccgtaaag gcggagatgg tcgcgctgct ggtaatactt ttccctgagt tcaggggtgt
    16741 tatactccgg gttttcaagg gtttgctcca tgtttaaatc cattggtacc tggagcacag
    16801 caaacccacc cggctttagc acccggagta tttcggccat cgccctaagg tcgttatcaa
    16861 cgtgttcaag cacatggttg caaatcacca tatcaaaggt attgtcggca aaaggcattg
    16921 cctgaacatc gagctttaca tcggcccagg gcgaaatgag gtcggctgtg gtatagtcaa
    16981 tgtttcttag tcgctttatc ggttttatga aacagtattc cggggcaaca tgcagaacct
    17041 ttttgggggc tgtgaaaaag tcggttttcc tttgcaggta aagccatatc agccggtgcc
    17101 gttccagcga catggagttt ggcgccagtg cgttttcccg tgtggtaacc cgcccgtagg
    17161 ggagtagctt gcgatactta accccatcga taggatcctg aaaccgactg ccccggtaaa
    17221 aaagtccaat ggctttcatc agaaacgatg caaaaagttg aaggtagtgc cggggtatga
    17281 accgggtggt aaacgaaact aaacgactta tcattcttgg gtttctactc tttaagtaac
    17341 tggtcaattt tttcgatata gtcgagcgaa tcgtcgatga aaaaggtaag ctcgggtatc
    17401 accctaagct ggtgccttac ccttttgccc agttcgcccc taaggaattt tgagttatcc
    17461 ctgatatgct cgagcgtttt atcggtatcg gttgagggga atatactaac gtaaacctta
    17521 gccaccgaga ggtcggggct aaccctaacc tgcgtaaccg taagcatttt accgggaaac
    17581 ctggcagccc cctcgcgctg gaaaatctcg ctaagctcct tctgtatgag ccgtcccacc
    17641 ttctgtaatc gtgttccttc catttccgta ttttttacaa agatataacg attttatgag
    17701 gtttgggtaa gcggaacaat gattggtgta atacttgtat tttcaattgc ttgctgctgt
    17761 ttgatttttc gtaccgctag ttaactatcc cacttgtcat cctgagcgta gcgaaaaa
//
LOCUS       DAOOWJ010000055        17749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3215_length_17749_cov_7.351079, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000055 DAOOWJ010000000
VERSION     DAOOWJ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 17749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17749
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3215_length_17749_cov_7.351079"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /gene="truB"
                     /locus_tag="PLW22_06825"
     CDS             <1..547
                     /gene="truB"
                     /locus_tag="PLW22_06825"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOU74455.1"
                     /translation="ATGLLVICTGKATKRVMELTADNKEYIAKIKLGETTPSFDLETD
                     VDAVYPFDHVNRELVETVLRTFSGWQLQEPPAFSAKYVNGKRAYEYARKGVDPKLEPV
                     PIEIHSIELIGFDLPFLTIKIVCSKGTYIRALARDIGKALSTGAHLVELRRTRSGIFS
                     IEQALSIAEVEKKLSDLGNQS"
     gene            682..1734
                     /gene="queA"
                     /locus_tag="PLW22_06830"
     CDS             682..1734
                     /gene="queA"
                     /locus_tag="PLW22_06830"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOU74456.1"
                     /translation="MKLSQFKYNLPKDLIAKFPVENRDESRLMVLNRKTGKIEHRIFK
                     DIIEYLNEHDVLVFNNTKVFPARLYGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGEDDVLVAEVIDNTTSRGRTLRFLFDGSYEEFKETLYRLGEMPVPKHI
                     LQREAVPEDRERYQTVYAKHEGAVAAPTAGLHFSKHLLKRLEIKGINFAEITLHVGLG
                     NFRTVDVEDLTKHKMDSEQIWISDDAANIINKAKREQHNVCAIGTTVLRTLESSVTTD
                     GFLKPYSGWTNKFIFPPYEVAVPNMLVTNFHLPLSTLLMMVCAFGGYDQVMDAYKVAI
                     KEKYRFGTYGDAMLII"
     gene            complement(1800..3419)
                     /locus_tag="PLW22_06835"
     CDS             complement(1800..3419)
                     /locus_tag="PLW22_06835"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOU74457.1"
                     /translation="MATPEFFYQERFPLEKDDTDYYLLTKDFVSSAKFEGEEILKVDP
                     EGLAFLARTAMHDISFFYRAEHVKQIAAILEDPEASKNDKFVALVLLRNAEIAAKGVL
                     PLCQDTGTATVFAKKGQRVWTGVNDYEALSRGIYKTYTEENLRYSQTVPLDMYTEKNS
                     GTNLPAQIDIYSTHGNEYEFLFIAKGGGSANKSMLFQETKALLNPEKLEAFLVEKIKN
                     LGTAACPPYHIAIVIGGTSAEDTLKTVKLASAKYLDNLPTSGNIHGRAFRDKELEEKI
                     LKATNSIGIGAQFGGKYLALDIRIIRLPRHGASCPVGIGVSCIADRNIKAKINSQGIW
                     IEKLEKNPGKYIPDQWRKPEENDAVGIDLNQPMQAILSELTKYPVGTRLSLTGPIIVA
                     RDIAHAKLKERLDKGEGLPEYVKNHPIYYAGPAKTPAGMPSGSFGPTTAGRMDSYVDT
                     FQSQGASLVMIAKGNRSQAVTDACKKYGGFYLGSIGGPAAILAQENIKKVELIEFPEL
                     GMEAVYRIEVVDFPAFILVDDKGNDFYKQIK"
     gene            complement(3440..4291)
                     /locus_tag="PLW22_06840"
     CDS             complement(3440..4291)
                     /locus_tag="PLW22_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOU74458.1"
                     /translation="MKLGNLILAALTPLLFSPTAFSQNADLVSMENEILKIVNEHRKS
                     IGKSELVFNDYIRKEAYIHSQNMASGKVTFSHKGFDERFNRLSGFLEITTGAENIANG
                     PLNAKHIVNGWLASPPHRKNIEDDFNLTGIGIAKAPNGSYFYTQIFVKSAEKPKVVPR
                     EYEAELLQLINNHRKGLGLPAIKNDEGIHTEALGYSQQMATGKVPIGPPSFDNPIKSL
                     MRRYKASKMVELIGYDYLKPKELFDAWMSSTSQRDIIEGNFNLTGIGVYQSVNGKVFV
                     TQVFLLQ"
     gene            complement(4304..4651)
                     /locus_tag="PLW22_06845"
     CDS             complement(4304..4651)
                     /locus_tag="PLW22_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOU74459.1"
                     /translation="MAMIRVLHNPRCATSRKGLEYLNSKNINFEVINYLSTGLSRDIV
                     KEILLKSNLKPIELVRTQEEYYKKFLKGKNFNDEEWIEILTQNPKLLKRPVVIGKYKA
                     VIGIPVENIDKVL"
     gene            4777..6246
                     /locus_tag="PLW22_06850"
     CDS             4777..6246
                     /locus_tag="PLW22_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74460.1"
                     /translation="MNKPYHTLGFLLLVAILYTLLSIIVPGSTFRVDDFLFRFPKPFE
                     QLAGDNIKIASSKTDSIQPDVNSKIETIAEESIPNLDSLYVEPVRASSDSAIAPPKSI
                     VTAGTLHLEMPQGINALGSFISAIYRNSDEVTTVRVLHYGDSQIEGDRITSQLRAALQ
                     EKFGGKGLGLIPIWNTEYLPYGFRVSLSEHVNVNSTVKAKPTQKPDCVLGSIAEVAPS
                     NLYREIVSIKVNSKQSFNTLSIISGNSDSSYAFKIIVDGTDTLETQVVPYSKVKLVKV
                     NLPSRFERISIQLRNPSTVKLYGLLAESGSGVSVCNIALRGTSGNFFNHFDSEVASEI
                     YRILNVRLVIMQFGINVVPSNLSSYAYYQKLMEQQIKAVKCFAPQADILVVGVTDMAN
                     RFDGELRSNPSVGKVLQAQRYAALGNGALFWDGYSAMGGEGSIIEWVNSKPPLATKDY
                     THFTNYGAKRFGQKMANALLMALGNNNQTTAFPDSVSHE"
     gene            6239..7426
                     /locus_tag="PLW22_06855"
     CDS             6239..7426
                     /locus_tag="PLW22_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOU74461.1"
                     /translation="MNRLVLHITGIAIFLILTLLPYQVSLLVPTKSPAILLFNHSANT
                     IKNFFGQKIVVKPSKPLTIVHIGDSHVQAGILSRTVKMFLAREFQSQYVSPGLVFPYS
                     VMGSNNPVEYTSYHTGRWEYQKVNGVKAPVDAGIFGVAVKTTDSLATISFKVKPNALF
                     KTQVNRVGVFYQSASSYLTPILVEPKNVNPILHENFVEFELESEVDSVVIGFRAKNDF
                     ECTVYGVNLWNTKSKFSLSSVGLNGATVHGFSLAKQLLPNIKLIKPDIVIVSLGTNDA
                     YSNLFDSLNFARDQENLINTIKSALPNPLIILTTPNDHLLNRKYMNIRVQVASNVIKE
                     ISTKHQLPVWDFYSLMGGKGSIQGWIRYGFAAPDGVHLTAKGYQLQGELFYHALYNSG
                     FIE"
     gene            7436..8965
                     /locus_tag="PLW22_06860"
     CDS             7436..8965
                     /locus_tag="PLW22_06860"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOU74462.1"
                     /translation="MINIKEIVLNFLHYTEGDSLMFTQLSFWVFFGVLLAGYTFVYNR
                     PVIRSAYLLVFSLFFYFKSSGLFFSLLLFSTLVDYTIGLAIAKSGSKINRKLLVALSV
                     LVNLLVLSYFKYAYFIVDLLNNLLGINLTVVNHLALWGNALLNTKFDTTTIVLPVGIS
                     FYTFQTISYTVDVYRGKVAPVRNIIDFAFYVSFFPQLVAGPIVRAAEFIPQIYRRYEL
                     SKNEFGHALFLVLAGLVKKMVISDYLSVNFVDRVFSNPLSYTGFENLLATYGYAMQIY
                     CDFSGYTDIAIGVALLLGFKLPINFNSPYKATSLTDFWHRWHISLSLWLRDYLYIPLG
                     GNRKGRIRTYINLLLTMLLGGLWHGAHLKFVVWGGIHGVGLAIEKLLSNLKLVNINSE
                     RHSTRLIWGIITFNVVCLSWIFFRANDFNAATDMLSQIFTSFGTKTIPDIALAFSDVF
                     LVLALGFIVHLLPYNAKEYLRGRFIAMPLSLKLFAALLVVVVLIQFQTMELKPFIYFR
                     F"
     gene            complement(8994..9067)
                     /locus_tag="PLW22_06865"
     tRNA            complement(8994..9067)
                     /locus_tag="PLW22_06865"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9033..9035),aa:Gln,seq:ttg)
     gene            complement(9143..10705)
                     /locus_tag="PLW22_06870"
     CDS             complement(9143..10705)
                     /locus_tag="PLW22_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOU74463.1"
                     /translation="MNTTVNVSIGGYSFTLEENAYNTLQEYLNTVKVKLGNDKVAQET
                     VNDIEFRIAELLTEKITLGQVVTNEMVTPIIEQIGKPEDIGENNDSTSNKQYRPKRLY
                     RNPDDSIIAGVCGGLAAYFSVDPLVFRILFFALLFLKGFGLLLYILLWIAMPKAKTPF
                     QKMEMMGDTENIDEIGRRVRKEISNASDNLKKSKAGNTVNKLINLFGQVMLYLLKAML
                     VIVKVISITIGVLLIVSMLLIFIALVGAVFFASYSPDFSGVIGGVGMTLNEIIASVLD
                     ISSSYWITIPLFLTLAIPIVALIYAGIRILFRFRAKDGTIGIIATVIWAAAVVTLSLT
                     FFFQMRGLSVSEKVVKELEISPVNSNSKIVLCKSYNYMTDSTSSIEEKVQIFDWKIVT
                     LKGKHQLAGSPTLIIDKGFEAKPRFEVIRKARGVNSYTAADNAKDIIYSLTLNDTLAI
                     LDPLYIIPEKTKWKNQSVLIKLYLPEGYGVYLDETLTEMLEKYQPYSNYWPDEMVGKT
                     WIMTANGLRIAR"
     gene            complement(10748..11089)
                     /locus_tag="PLW22_06875"
     CDS             complement(10748..11089)
                     /locus_tag="PLW22_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOU74464.1"
                     /translation="MNQEIENAKAQMRKGVLELCVLTVLSRGDAYANDLINQLKQAKM
                     IVVEGTIYPLLTRQKNAGLLTYRWEESPQGPPRKYYSLTEEGKEYLAELLNSWDELVE
                     MVSAIRNQKND"
     gene            complement(11163..11696)
                     /locus_tag="PLW22_06880"
     CDS             complement(11163..11696)
                     /locus_tag="PLW22_06880"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOU74465.1"
                     /translation="MKRTISVSLNGKAYMLEDDAYHALDRYLKGLEAHFANDPNKKEI
                     VGDIESRIAEHFDEFIKVPGQVVNLEEVNRVVHLMGNINDFDPTNTKASSENDRVYPK
                     LYRDVEDRILGGICTGMGHYWKVDPVLFRLVFFLLTLWGGLGVLIYIVLWVVVPPAIT
                     PTQKLEMKGEKINVNNM"
     gene            11971..12777
                     /locus_tag="PLW22_06885"
     CDS             11971..12777
                     /locus_tag="PLW22_06885"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HOU74466.1"
                     /translation="MKGVLTKMGFTNTGNDVSISPEYFLDLDSGRVNVSELIGKGIGL
                     KYLNINRCIHCGRETKKLFGQGFCYPCFIKVPEAEECVLRPELCRAHEGIARDLEFAK
                     ANCLVQHYVYLAWTGGLKVGVTRYHQIPTRWVDQGATFAVKICHTPNRYTAGLAEVEL
                     KKIFADKTAWQRMLTDSSELPPDFLTHKAKALEFINGKNLSYIPESDVVYRVSYPVIQ
                     LSDKVRSVNLEKVNSIEGKLVGIKGQYLIFESGIVINIRKHSGYLVDITF"
     gene            complement(12774..15065)
                     /locus_tag="PLW22_06890"
     CDS             complement(12774..15065)
                     /locus_tag="PLW22_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOU74467.1"
                     /translation="MRLKLTIRQKILYYILGSAFIIFSIVFYFISSSSRRLAYEQSIK
                     LTNSYARQYALNIESWINQDFAVTRTLAAAFMEYKQMPFDQWQKLIYPMYNRVILTTP
                     HIDAYWDSWELSNLDPNWTLPYGRHFYIVYKQDGIYKTKAEMRSLTGDPPTYGSMKSA
                     AKEIIVEPYTSELQRGQMMTTLSSPLLENGKFIGLIGADLILTRFQQLVNTIKPYPNS
                     YAFLLSYKGVFIAHPDSSIFKKNITEVLPEFNNQHNILAQVQKGKAFSFTHQNNKGDR
                     IYYTFEPIRIGNTQTPWSIGIAVPVTDIMAQANRSYNTSLFIALVGLLVIIFMVYFVS
                     NNITLPIKKITQILNEFAKGKIVSSIDISTSSNDEISQMVEAVSASLAGVNSKTSFAR
                     EIGAGNLNVDLALLSDEDQLGKSLIEMRDNLRRARDEEEKRKIEEEKKRWTNEGMAKF
                     GEILRQNNNNLSLLSQELIKNLVWYLNAQVGGLFVLSENKETGDKTFDMVAMFAYDRN
                     RVMQRSYHFAEGLIGACAAERDAIILKEVPQDYIEITSGLGGINPNYIIVVPLIFEEE
                     VMGVIEIASLQELKEHEVQFLKDIAKSIASTLHTVKVNTLTAELLMKSKEQAEMMAAQ
                     EEEMRQNMEELQATQEEAARKTLELEGLVNALNAAAFVMEYDTKGYVININDSYLNFL
                     GANRDDIIGLHHAENLILSETEKASYDKMWSDILRGNIRKHKTRIRVNGQEHLLLETY
                     TPIFDHGGQLLKILKIATDITNI"
     gene            15180..16403
                     /locus_tag="PLW22_06895"
     CDS             15180..16403
                     /locus_tag="PLW22_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU74468.1"
                     /translation="MLNLFKKHRDDELEKRIDCLRSILLFKEVDHSTLRKIASVLESC
                     EVKKDERLFNKGDRDYSLYIVVSGKVKAHDGNHTFATFGPNQYFGEYSLLDSSARSTA
                     VTAMEDSVLLRLHQEQFNRFLQKIPSLSRTLLLGLVWRLRDYNVLEAELTAKNLEIKK
                     QKEEIEQQSKELEQLNQMKDKFFAIIAHDLRNPFSTVLSLSELLAKEFANFDQNNLQM
                     FIEQIYKYSNKTFNLLENLLQWSMLQTGRLTPKPQMANLADVINDTIDLLKGTALNKS
                     INLYWALPTDSFAYFDVNMITTVVRNLISNAIKFTPQNGEVVVQVDAEPDHYKVSVID
                     TGVGIPDEVIQKLFRIDSNPTTLGTNEESGTGLGLILCKEFVEKNGGTIGVRSVVSKG
                     STFYFTVPRIEVKKQ"
     gene            16400..17494
                     /locus_tag="PLW22_06900"
     CDS             16400..17494
                     /locus_tag="PLW22_06900"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HOU74469.1"
                     /translation="MKVAILGTRGIPNRYGGFERFAEELSQELNKRGYTVYVTSPATH
                     SCKELNANNIFTVNLRVPRFLTSNLATLYYDYISLKWAIRNNIDAVIECGHSFAPLLL
                     FISGSIRRKIITNPDGIEHKRKKWGWIAKCYLRFSEYLAFKLSAQLVCDNRALVQYYS
                     TRYKRRIHYIPYGAHPLMQAPGRPDVLDKLHINEYYLLITRITPENNVETVLSYFAHS
                     DQSCLVVGDLTSSYAKKIVGKYSHFRNIHLLGAIYDQQILNGLRFYCKAYIHGHSAGG
                     TNPSLLEAMACGCFIIAHDNPFNRDILGEQGLYFNNIATLGSCISQFEKLNDVEVNRV
                     KVQNIQAIERNFLWEKVALAYIDVIDMLKK"
     gene            complement(17525..>17749)
                     /locus_tag="PLW22_06905"
     CDS             complement(17525..>17749)
                     /locus_tag="PLW22_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU74470.1"
                     /translation="PTTGNFSIKITGFEPGRYKLEFYNAIGNKVRSRDITIENTETDI
                     TDNMERLPKGLYYIRFGNSKKYLIRKLILE"
BASE COUNT         4974 a         3603 c         3651 g         5521 t
ORIGIN      
        1 ggctacgggc ctattggtaa tttgtaccgg aaaagctacc aaaagggtta tggagcttac
       61 ggccgacaat aaggagtaca ttgctaaaat caagcttggc gaaacaaccc catcgttcga
      121 cttagagacc gatgttgatg ctgtttaccc ctttgaccac gtgaatcgtg aactggttga
      181 aactgtttta aggacatttt caggatggca gttgcaggaa ccccctgcgt tctcagcaaa
      241 gtatgtcaac ggaaaacgtg cctacgagta tgctcgtaag ggtgtcgatc ctaagcttga
      301 accggttcca atcgagattc atagcattga gcttatcggt tttgatttac cgtttctaac
      361 aataaaaata gtttgtagca agggtaccta catcagagct ttagcccgcg atattggtaa
      421 ggccctaagc acaggagcac atctggttga gttacggcgt acgcgtagtg gtattttctc
      481 aattgagcaa gccttatcaa ttgctgaggt ggaaaaaaaa ctatctgatt taggtaacca
      541 aagctaaata ttgccgtata aggtgcacat gctctaaaat aatagattat ctttgcaaat
      601 tcctataaat taagggtaaa actgttgaca acacctggtg gcaagttata tctttgtgta
      661 aattgaagta aaactataca tatgaagcta tcgcaattta agtacaatct gccaaaagat
      721 ttaattgcaa aatttcctgt tgagaacagg gatgaatcga ggctaatggt gttgaacagg
      781 aaaaccggga aaattgaaca tcggattttt aaggatatta ttgagtatct gaacgaacac
      841 gacgttttag tattcaacaa cacaaaggtt tttcccgcac gcctatacgg caataaggaa
      901 aagactgggg ctgaaataga ggtgttcctt ctccgtgagc tgaaccggga gcaaaggcta
      961 tgggatgttt tagttgaccc tgcccgtaaa atccgaatag gcaataagct ctattttggc
     1021 gaagatgatg ttctggtagc tgaggttatt gacaatacca ccagccgtgg acgtaccctg
     1081 cgtttccttt tcgatggttc ctatgaggag tttaaggaaa ccctttacag gttaggcgag
     1141 atgcccgttc caaagcatat cctacagcgc gaggctgtac ccgaagatcg tgagcgttac
     1201 caaaccgttt atgccaagca cgagggtgca gttgcagctc ccaccgcagg tctgcatttc
     1261 agcaagcatc tgctcaaacg gcttgagatt aaaggaataa actttgccga gattaccctt
     1321 catgttggct taggcaactt cagaactgtt gatgttgagg atttgaccaa gcacaaaatg
     1381 gattcggagc agatttggat tagcgacgat gccgccaaca tcattaacaa ggccaaacgt
     1441 gagcagcaca acgtttgcgc tattggtaca accgtgctac gcacccttga gagctctgtg
     1501 acaaccgatg gcttcctaaa gccatacagc gggtggacta acaagtttat cttcccgcca
     1561 tacgaggttg ctgtaccaaa catgctggtt acaaacttcc acttgccttt atcaaccctc
     1621 ctaatgatgg tttgtgcctt tggcgggtac gatcaggtta tggatgctta caaggttgcc
     1681 attaaggaga aataccgctt tggcacttac ggcgatgcca tgctgatcat ttaaaaagaa
     1741 aatactaaaa gcaaaaggcc cggttatcgg gcctttttta tttcgaaagt cttaaggaac
     1801 tacttaatct gcttgtaaaa gtcgtttcct ttgtcatcaa ccagtataaa tgccgggaaa
     1861 tcaaccacct caattcggta caccgcctcc attcccagtt cagggaactc aattagttca
     1921 acctttttaa tgttttcctg ggccagtatg gctgcaggac cgccaatgct cccgaggtag
     1981 aaacctccat atttcttgca ggcatcggtt acagcctggc tgcggttacc cttggcaatc
     2041 atcaccaggc ttgcaccctg cgattggaat gtgtcaacat atgaatccat tcgccctgca
     2101 gtagttggac caaacgaacc cgagggcatt cccgctggtg tttttgctgg accagcatag
     2161 taaatagggt gatttttaac gtattcgggt aacccctcac ccttatctaa acgctctttt
     2221 agctttgcat gggcaatatc gcgggctaca attattggcc cggtgagcga aagccttgtg
     2281 cctacagggt acttggtgag ttccgaaaga attgcttgca tgggttgatt caagtcgatt
     2341 cctacagcat cgttttcttc aggcttacgc cattggtcgg ggatatattt gcccggattc
     2401 ttttccagct tctcaatcca aataccttgg ctgttaattt ttgcctttat gtttctatcg
     2461 gcaatacatg atacccctat tcctaccgga catgatgcac catgcctggg aaggcgtata
     2521 attcgaatgt caagggcaag gtatttaccg ccaaactgag caccaattcc aatactgttg
     2581 gtggctttta gaattttctc ttccagctct ttatccctga atgctctacc gtgaatgttc
     2641 ccggaggttg gcagattatc gaggtatttt gctgaagcca actttactgt cttcagtgta
     2701 tcctcggccg atgttcctcc aattactatg gcaatatggt agggtggaca agcggctgta
     2761 cccaaattct taattttttc aactaaaaat gcttccagct tttcggggtt cagtagtgcc
     2821 ttggtttcct ggaaaagcat acttttattg gcggaacctc ccccttttgc tataaagagg
     2881 aactcgtact cattgccgtg agttgagtaa atatcgattt gggcaggtag gtttgtcccc
     2941 gagttcttct cggtatacat gtcaaggggt acggtttggg agtaacgcaa attttcctcg
     3001 gtgtaggttt tgtagatacc acgcgaaagt gcctcgtagt cgttgacgcc agtccaaacc
     3061 ctttgcccct ttttggcaaa cacagtagca gtacctgtat cctggcatag cggtaaaacc
     3121 cctttggctg caatctcggc gttgcggagc aataccaacg ctacaaactt atcgttttta
     3181 ctggcttccg gatcttccag aattgcagca atttgcttta catgttcagc cctgtaaaag
     3241 aaagagatgt cgtgcattgc ggtgcgggca agaaatgcca aaccctccgg gtcgaccttt
     3301 aaaatctctt cgccctcaaa ctttgctgat gaaacaaaat cctttgttag gagatagtaa
     3361 tcagtatcat ccttctccaa aggaaatctt tcctggtaaa agaattccgg tgttgccatg
     3421 gtatttagtt ttaggttggt tactgaagca gaaacacctg ggtaacaaac actttaccgt
     3481 ttactgactg gtaaactcca attcccgtta ggttgaaatt accctctata atatcgcgct
     3541 ggctagtgct gctcatccag gcatcaaata gttccttggg tttgaggtaa tcatatccaa
     3601 taagctctac catttttgat gctttatacc gacgcattag cgatttaatt ggattgtcga
     3661 aactcggtgg gccaatagga actttgcctg tagccatttg ctgggagtac ccgagtgcct
     3721 cggtatgaat accttcgtcg ttttttatgg caggtagccc aagaccctta cggtggttat
     3781 ttatcagctg taaaagttct gcctcgtact ccctgggtac aaccttaggt ttctcagcac
     3841 tttttacaaa tatctgggta taaaagtatg agccatttgg ggcttttgct attcctattc
     3901 ctgtaagatt aaaatcatct tcaatatttt ttctatgggg aggactggca agccatccgt
     3961 ttacaatatg ttttgcgttt agggggccat ttgcaatgtt ttcggctccg gttgtaattt
     4021 ccagaaaacc gcttaaccga ttaaagcgtt catcgaatcc cttgtggcta aatgttacct
     4081 tacccgatgc catgttctgg gaatgaatgt atgcttcttt gcgaatataa tcgttaaaca
     4141 ccaattccga tttaccaatc gatttcctat gctcgttcac tattttaaga atttcattct
     4201 ccatgcttac caaatcggca ttttgcgaaa atgctgttgg ggagaataat agcggtgtaa
     4261 gtgcggctaa aattaaattt cctaatttca tggctatgga agtttataaa actttatcga
     4321 tattttctac aggtatacct attacagctt tatatttacc aatcactact gggcgtttta
     4381 aaagttttgg gttttgagta agtatctcta tccattcctc atcgttaaaa ttcttgcctt
     4441 tcagaaattt cttgtagtat tcctcctgag tccttaccag ttcaatgggt tttaggtttg
     4501 atttaagcaa aatctctttc acaatatcgc gacttaggcc ggtggacaaa tagtttatta
     4561 cttcaaaatt gatatttttt gagttcaggt actccaaccc ctttcggctg gttgcgcatc
     4621 gtggattatg taacactctt atcattgcca ttataacatc ctgtaagtta ctatttatca
     4681 aatttaaaag caaaattact ttccaattat tgctacctaa tatacaccat ttaacaaaaa
     4741 caattacatt taaaatccgt tagcattaaa catttcatga acaagcctta ccatacgtta
     4801 ggtttcctgc tgttggtagc aattctttac acattactat ccataattgt gccaggtagc
     4861 acctttaggg tagatgattt tttgtttcgt tttccaaaac cctttgagca attagctggg
     4921 gacaatataa aaatagcatc tagcaaaacc gattccattc aacccgatgt aaattcaaaa
     4981 atagagacaa tagcagaaga gtcaatcccg aatttggatt cattgtatgt agaacctgta
     5041 agagcgagta gcgattcagc aatagccccc ccaaaaagta ttgttacagc cggaacctta
     5101 caccttgaaa tgccacaggg tattaatgcg ttaggctcct ttattagcgc tatatatagg
     5161 aatagcgatg aagttacaac ggttcgagtg ctgcattatg gcgattcgca aattgagggc
     5221 gataggatca ccagccagct gagagctgcc cttcaggaaa agttcggtgg gaagggtttg
     5281 ggtctaattc caatttggaa tactgagtat ttgccatacg gctttagggt tagcttatca
     5341 gagcatgtaa atgtaaattc aacggttaag gccaaaccaa cacaaaagcc cgattgtgtg
     5401 cttgggtcaa ttgctgaggt tgccccatcg aatttgtatc gcgagattgt ttccataaag
     5461 gttaactcta agcaaagctt taacacctta agcataattt caggtaactc tgattcttcc
     5521 tatgcattta aaattatagt tgatggaacc gataccctag agacgcaggt tgtgccttac
     5581 agcaaagtga aattggtaaa agtaaacctt cccagccgtt ttgagagaat ctcaattcaa
     5641 ttaaggaatc catctaccgt taagctttat ggcttactgg ccgaatcggg cagcggtgtt
     5701 agtgtttgca atattgcatt aaggggaaca tccggtaact tttttaacca tttcgattca
     5761 gaggttgcct cggaaattta cagaatacta aatgtccgat tggttataat gcagtttgga
     5821 atcaatgtgg ttccctcgaa cctatcaagc tatgcctact accaaaagct tatggagcaa
     5881 caaataaaag cagttaagtg ttttgctcca caagccgata ttttagttgt tggggttacc
     5941 gatatggcca accggttcga tggcgagctg agatcgaacc caagcgtggg aaaagtgctg
     6001 caggctcaaa gatatgcggc tttaggtaat ggggctttgt tttgggatgg gtattccgct
     6061 atgggtggag aaggttcaat aattgaatgg gttaactcta aacctcccct tgcaacaaag
     6121 gattataccc actttacaaa ttatggggct aagcgctttg gccaaaaaat ggctaatgca
     6181 ttgctaatgg ccttaggtaa taacaaccag accactgctt tccctgattc agtaagccat
     6241 gaataggtta gtacttcata ttaccggcat tgccattttt ttaatcttga cactcttgcc
     6301 atatcaggta agcctactgg tacccacaaa atcacctgcc attttattat tcaaccattc
     6361 cgctaacaca ataaaaaatt tctttggcca aaaaatagta gttaaaccca gtaagccgct
     6421 aacaattgtt catattggcg attcacatgt gcaggctgga atattatcga gaacagtaaa
     6481 aatgtttctt gcacgcgagt tccaaagcca gtatgtttcg cctggattgg ttttccctta
     6541 ctcagtaatg ggttcaaata atcctgttga gtacacctca tatcatactg gccgatggga
     6601 gtaccaaaag gtaaacggag tcaaagctcc agttgatgca ggtatttttg gggttgcagt
     6661 taaaaccacc gatagccttg caaccatatc gtttaaagta aagcctaacg ccctcttcaa
     6721 aacacaggta aacagggtag gtgtattcta ccaatcagct tcatcctact tgactcctat
     6781 tctggttgaa cccaaaaatg taaatcctat tttacatgaa aattttgttg agtttgaact
     6841 tgagagcgaa gttgatagtg ttgtaatagg gtttagggca aaaaatgatt ttgagtgtac
     6901 agtttatggt gtgaatcttt ggaacacaaa atcgaaattt tctcttagtt ctgtaggctt
     6961 aaatggtgca actgtccacg gattttcatt ggcaaaacaa ttgctcccca atatcaaatt
     7021 gattaaaccc gatatagtaa tagtttcact tggtaccaat gatgcatatt caaacttatt
     7081 cgatagtctc aattttgcac gcgatcagga aaatttaatt aacacaataa aaagcgcttt
     7141 acctaaccca ttgataattc ttaccactcc aaacgatcat cttttaaatc gaaagtacat
     7201 gaacatcagg gtacaggtgg cgtccaatgt tattaaagag atttcaacca aacatcaact
     7261 tccggtgtgg gatttctata gccttatggg tggcaaggga agtattcagg gatggattag
     7321 gtatggtttt gcagcccccg atggggttca tcttacagca aaaggttatc agctccaagg
     7381 cgagttgttt taccatgcgc tgtataattc aggttttatt gagtagccaa ttgttatgat
     7441 taatattaag gaaatagttc tgaactttct tcactacacc gagggtgact ccctgatgtt
     7501 cacccagctt tccttttggg ttttttttgg cgttttactg gctggttaca cgtttgtata
     7561 caaccgtccg gttatccgaa gtgcttacct tttggtattt agccttttct tctactttaa
     7621 atcaagcggg ttatttttct cacttcttct gttttcaacc ttggtcgatt acacaattgg
     7681 attagcaata gctaaatcgg gtagcaagat aaatagaaag ctgcttgtag ccttaagtgt
     7741 tttggtaaac ctattagtat tgtcgtactt taagtacgca tactttattg ttgatttgct
     7801 caataacctt ttaggcatca accttacagt tgttaatcat cttgcccttt ggggcaacgc
     7861 cctccttaat acgaaatttg acactaccac cattgtgctt cctgttggaa tttcattcta
     7921 tacctttcaa accataagct atacggttga tgtttaccgg gggaaagtcg ccccggtcag
     7981 aaacattatt gattttgcgt tttacgtttc gtttttccct cagctggttg caggcccaat
     8041 tgtgcgtgct gcagagttta ttccgcaaat ttaccggcgg tatgaattaa gcaaaaatga
     8101 gtttggccat gcactttttc tagtacttgc tgggttggtg aaaaaaatgg taatttccga
     8161 ttacctgtcg gtaaactttg tcgatagggt ttttagtaat cccctcagct atactggttt
     8221 tgaaaaccta ctggccacct atggctatgc catgcagatt tattgcgatt tttcaggcta
     8281 caccgatatt gcgataggag tagccctttt gcttggcttt aagcttccaa tcaattttaa
     8341 ctctccctac aaagcaacca gtctaaccga tttctggcac cgttggcata tatcgctatc
     8401 gttgtggttg agggattacc tttacattcc cttaggagga aaccgaaaag gaaggataag
     8461 aacctacata aacctattat taaccatgct acttggtggt ttatggcatg gcgcacatct
     8521 gaaatttgtt gtttggggtg gaattcatgg ggttggattg gcaattgaaa agttgctatc
     8581 caacttaaaa ttagtaaaca ttaatagcga gagacattca acccgattga tctggggcat
     8641 tatcaccttt aatgtggttt gcttgtcgtg gatttttttc agggcaaacg attttaatgc
     8701 ggcaaccgat atgctaagtc aaatatttac cagtttcggg actaaaacta tcccggatat
     8761 tgctctggct ttttccgatg tattcctggt tcttgcgttg ggctttattg tccacttatt
     8821 gccctataat gccaaggagt atctgcgggg taggtttatt gctatgcctt tgagtttaaa
     8881 gctttttgca gctctgttgg tagtagttgt tctcattcaa tttcaaacca tggagcttaa
     8941 gccgtttatc tatttccggt tctaaaataa aaaagcccct tattcagggg ctttggttga
     9001 cctaccagga ctcgaaccta gactaacaga accaaaatct gctgtgctac cattacacca
     9061 taggtcaatc ctatcgcttt tttaaagcag tgcaaaatta atactaatgt agtaaacctc
     9121 aaaatatttt ttaaaaattt atttacctcg caatacgcaa accgtttgcg gtcataatcc
     9181 atgttttccc aaccatttca tcgggccagt agttactgta gggttgatat ttttcaagca
     9241 tttcggttag cgtttcgtcc aggtaaacac cgtacccttc aggcaaatac agcttaatga
     9301 gtacgctctg gtttttccac tttgtttttt cagggataat gtataacgga tccagaatgg
     9361 ccagggtatc gtttaatgtt aaggagtaaa taatgtcctt tgcattatct gctgctgtat
     9421 agctgttaac ccctcttgct ttgcgtatta cctcaaatct tggtttcgct tcaaagccct
     9481 tgtctataat aagcgtgggt gagcctgcaa gctgatgttt cccttttagt gtaactattt
     9541 tccaatcgaa aatttgaacc ttttcctcta tcgatgatgt actatcggtc atgtagttgt
     9601 atgatttaca caatacaatc ttagagttgc tatttactgg cgaaatttcc aattccttta
     9661 ccaccttttc gctaacggaa aggcctcgca tctggaagaa aaatgtgagt gaaagggtta
     9721 caacagcagc ggcccaaatt acagtagcaa taatgcctat tgtgccatct ttagccctga
     9781 acctgaaaag aatgcgtatt ccagcataaa ttagtgcaac aattggtatg gccagggtta
     9841 aaaatagtgg gattgtaatc cagtagctac tactaatatc aagaaccgat gcaataattt
     9901 cattgagtgt cattccaacg ccaccaataa ctccggaaaa atcgggacta tacgatgcaa
     9961 aaaacacagc gccaacaagg gctataaaaa taagtagcat gctaacgatt aaaagaactc
    10021 ctatggtaat agaaattacc ttaacaataa caagcatggc ttttaacagg tagagcataa
    10081 cctgtccgaa aaggtttatt aatttattaa cagtgttacc tgcctttgat ttttttagat
    10141 tatcacttgc attgctaatc tcttttcgaa cacgcctgcc aatttcatca atgttctcgg
    10201 tatcgcccat catttccatt ttttggaatg gggtctttgc ttttggcatt gcaatccata
    10261 gtagaatgta aagtagcaag ccaaatcctt ttaggaataa aagagcaaaa aataaaatcc
    10321 taaaaactag ggggtcgaca ctgaagtagg ctgccagtcc accgcaaacg ccagcaataa
    10381 ttgaatcgtc ggggttacga tataaacgtt ttggacggta ctgtttattg ctggttgagt
    10441 cgttattttc accaatatct tcaggtttac ctatttgctc aattattggc gttaccattt
    10501 cattggtaac cacttgacct aaggtaattt tttcggtaag caattctgct attcggaatt
    10561 caatatcgtt cacagtttcc tgggctacct tatcgttgcc aagttttacc ttaaccgtgt
    10621 taaggtactc ctggagcgta ttgtatgcat tttcttccag tgtaaaagag tagcctccaa
    10681 tacttacatt tacagttgta ttcatttgtt ttttattaat ggtaaaaatg gtaatagtac
    10741 ctaaaaatta atcatttttc tgattgcgaa tagcgctaac catttcaacc agttcatccc
    10801 agctatttag cagttcagca agatactctt tgccttcttc ggttagcgaa tagtacttcc
    10861 ttggagggcc ttgcggcgac tcttcccatc gataggtgag taaaccagca ttcttttgcc
    10921 gagtaagtag ggggtaaata gtaccttcaa ctactatcat tttagcctgt ttaagctggt
    10981 ttataaggtc atttgcgtaa gcatcgcctc tcgatagtac tgttaaaaca catagctcaa
    11041 gtacgccctt tcgcatttgt gctttagcat tctctatttc ctgattcatg gcttcaactt
    11101 agggtttctg ttatgttgtg catattttct gattcaaccc attctttgct atgaaaaatg
    11161 ggttacatat tgttaacatt tattttttca cccttcatct caagtttttg tgttggggta
    11221 atggcgggtg gaacaactac ccataaaaca atgtagatta gtacgcctag acctccccaa
    11281 agggttagca ggaaaaatac caaacggaaa agaacgggat caactttcca gtagtgtccc
    11341 atacccgtgc atatcccccc taaaattctg tcttccacat cgcggtaaag ttttgggtac
    11401 accctgtcat tttcggaaga cgctttagtg tttgtggggt caaagtcgtt aatgtttccc
    11461 attaggtgaa ctacccgatt aacctcttca aggtttacca cttgccctgg aacctttatg
    11521 aattcatcga agtgttcggc aatcctactt tcaatatcgc caactatttc ctttttgttg
    11581 gggtcgtttg caaagtgagc ctccagaccc ttaagatatc gatctagggc atggtaggca
    11641 tcatcttcaa gcatataggc tttaccgttt aaactcactg aaattgtacg tttcatagct
    11701 catgctgttt ggtttgactt tttatttgtt gcaatgcaat aactagtatt tatttattac
    11761 aaatataagc gataaaataa tactatgcaa tacatagtag tatatttaac atggtttaac
    11821 attgcagtca ttttgtagaa ccttaagctt tctttttaaa tgtttatctt tataattcat
    11881 tggttgagct atgttcttta ttctgtgttt tagcaatttc agcgggcgag gatatataaa
    11941 attcaactta ctcattaaaa taaatctgcc atgaaaggtg tgcttacaaa aatggggttc
    12001 actaatactg gcaatgatgt tagtattagt ccagagtatt ttcttgatct tgattcaggt
    12061 agggtaaacg tttcagaact aattggaaaa ggaatagggc tgaagtacct aaatattaat
    12121 cgctgtattc attgtgggcg tgaaacaaag aagctgtttg gtcaggggtt ttgttaccca
    12181 tgttttataa aggtaccgga agcggaggag tgcgttttaa ggcctgaact gtgcagagca
    12241 cacgagggta ttgcccgcga tttggaattt gcaaaggcaa attgtcttgt gcaacactac
    12301 gtttacctgg catggactgg tggcttaaag gttggtgtta caaggtatca ccaaataccc
    12361 acccgatggg ttgatcaggg agcaaccttt gccgtaaaga tctgtcatac tcctaaccgt
    12421 tatactgcag gattggctga ggtagagcta aaaaagattt ttgccgataa aacggcttgg
    12481 caacgtatgc tcaccgattc aagcgagcta ccgcccgatt ttttgaccca taaagcaaaa
    12541 gcgcttgaat ttataaatgg taaaaattta agttacattc ctgaaagcga tgtggtttac
    12601 cgtgtaagct atccagtaat tcaactttca gataaggtaa gatccgttaa cctggaaaaa
    12661 gttaattcaa ttgagggtaa actggttggt ataaaaggac aatacctgat ttttgaatca
    12721 ggtattgtaa tcaatattcg gaaacattca ggatatttgg ttgatattac cttttaaatg
    12781 ttggttatat ccgttgcaat ttttagaatt ttcaacagtt gaccgccatg gtcaaaaatt
    12841 ggagtgtagg tctcaagaag tagatgttcc tgtccattaa ccctaatgcg ggttttatgc
    12901 ttacggatgt tacccctgag aatatcgctc cacatcttat cgtacgaggc tttttcggtc
    12961 tcggacagta taaggttctc tgcatggtgt agtccaatta tgtcgtcacg attggctcca
    13021 aggaagttga ggtaactatc attgatgtta atcacgtaac ccttggtatc gtactccata
    13081 acaaaagcag cagcattcag ggcattaact aacccttcga gttcaagggt tttacgggca
    13141 gcctcttcct gggtggcttg tagttcctcc atgttctgac gcatctcttc ctcctgggca
    13201 gccatcattt ccgcttgctc tttggatttc attaaaagtt cagcagtcag ggtattaacc
    13261 tttacggtat gcagggtcga tgcaatgctt ttggcaatat ccttgaggaa ctgaacctcg
    13321 tgttctttaa gctcctgaag cgaggcaatt tcaataaccc ccataacctc ctcttcaaat
    13381 atcaagggaa caactattat gtaattgggg ttaataccac caagccctga ggtaatctct
    13441 atataatcct gtggaacttc tttcaaaatg atagcatcgc gttcagcagc gcaagcacca
    13501 ataagacctt cggcaaaatg gtagcttcgt tgcataaccc tgttcctgtc gtaggcaaac
    13561 atggcaacca tatcgaaggt tttatcgcca gtttccttgt tttcgctcaa aacgaataac
    13621 ccaccaacct gagcgttaag gtaccaaaca aggtttttaa taagttcctg gcttagcagg
    13681 ctaaggttat tgttgttctg acgaagtatt tcaccaaact ttgccattcc ctcattggtc
    13741 caccgcttct tttcctcctc aatttttcgt ttttcttctt catcacgagc tctacggaga
    13801 ttatcgcgca tttcaataag cgacttacca agttgatcct cgtcgcttag cagcgcaagg
    13861 tcaacattta ggtttcctgc accaatctcg cgggcaaatg aggtctttga gtttacacca
    13921 gcaagcgagg cgcttaccgc ctcaaccatt tggcttattt catcgttcga tgaggtacta
    13981 atgtcaatgc tgcttacaat ctttcccttt gcaaactcat taagtatttg ggtgattttt
    14041 ttaatgggca atgtaatgtt gttggaaaca aagtaaacca taaaaataat taccagtaaa
    14101 cctaccaagg cgatgaagag gcttgtgttg taactcctgt ttgcttgagc cataatatcg
    14161 gttacaggaa cagcaattcc aattgaccaa ggtgtttgtg tattacctat tcggatgggc
    14221 tcaaaagtgt agtaaatcct atcacctttg ttattttgat gggtgaagct aaaggctttc
    14281 cctttctgaa cctgggctag tatgttgtgt tgattattaa actcaggtaa tacttcggtt
    14341 atattctttt tgaaaattga ggaatcgggg tgggcaataa aaacaccctt atatgatagt
    14401 aaaaaggcgt agctattggg gtaaggttta atggtattta ccagttgctg aaagcgggta
    14461 agtattaggt cggcaccaat taaaccaata aacttaccat tttcgagaag tggcgatgag
    14521 agggtggtca tcatttgtcc ccgttgaagt tccgatgtgt atggttcaac aatgatctcc
    14581 ttggcggcac ttttcatact accgtaggtt gggggatcgc cggttaagct gcgcatttca
    14641 gcttttgttt tgtaaatccc atcttgctta taaactatgt agaaatgccg accataaggg
    14701 agtgtccagt tggggtcaag attgcttagc tcccagctat cccagtaagc atctatatgt
    14761 ggagtggtta gaataaccct attgtacatg gggtagatta gtttttgcca ttgatcaaag
    14821 ggcatctgct tgtactccat gaatgctgca gcaagcgtcc gggttacagc aaaatcttgg
    14881 tttatccaac tttcaatgtt aagggcgtac tgtcgggcat aactattggt tagcttaatg
    14941 gattgctcgt atgctaaacg gcgcgacgat gaacttatga agtaaaaaac tattgaaaaa
    15001 ataataaatg cactacccag gatgtagtat agtattttct gccggatggt aagttttaat
    15061 ctcatagccg ttttaattat gccatttacg tttcacaata ttacaaaaaa atgattgttt
    15121 atgcagatat taaggaattt tatttaattt ggcagtacca ttttattcaa aaaatttcca
    15181 tgctaaattt atttaaaaaa catagggatg atgagctcga aaaaaggatt gactgtttaa
    15241 ggtcaattct tcttttcaag gaggttgatc attcaactct tcgaaaaatt gcatcggtac
    15301 ttgagtcttg tgaggttaaa aaagacgaac ggttattcaa caaaggcgat agggactact
    15361 ctttatacat tgtagtaagc ggaaaggtta aagctcacga tggcaatcac acctttgcca
    15421 cctttggccc aaatcagtat tttggagagt actcacttct cgattcctcc gctcgatcca
    15481 ctgcagtaac tgccatggag gattccgtgt tacttaggtt acaccaggaa caattcaata
    15541 gatttttaca aaaaatacca agtttatcca gaactctttt actcggttta gtttggcgct
    15601 taagggatta caatgtactt gaggcagaac ttacggctaa aaacctcgag ataaaaaaac
    15661 aaaaggagga aattgaacaa cagagcaagg agctggaaca gctgaaccaa atgaaagaca
    15721 agttctttgc aatcatagcc catgatcttc gaaatccttt cagcacagtt ctttccctat
    15781 cggagttgtt ggctaaggag tttgccaatt ttgaccaaaa caacctgcaa atgttcattg
    15841 agcagattta caagtactca aacaagacat ttaacctgct cgagaacctt ttacagtggt
    15901 caatgctaca aaccggaagg ttgacgccta aacctcaaat ggcaaacctg gccgatgtta
    15961 taaacgacac cattgatttg ctaaaaggaa cagcacttaa caaatctata aacctatact
    16021 gggccttacc aaccgattca ttcgcatatt ttgatgttaa tatgattaca acggtggttc
    16081 gtaatcttat tagcaatgcc ataaagttca cacctcaaaa tggcgaggtt gttgttcagg
    16141 ttgatgctga acctgatcac tataaagtct cagtaattga tactggggtc ggcatacccg
    16201 atgaggtaat tcaaaaactt ttccgcatcg attcgaaccc aaccacttta ggaactaacg
    16261 aggagagcgg tacgggattg ggtttaatac tctgcaaaga gtttgttgag aaaaacggag
    16321 gtacaattgg ggttaggagt gtggtaagca aaggttcaac attttacttc actgtgccgc
    16381 gcattgaagt gaaaaagcaa tgaaagtagc tattcttggc actcgcggta ttcccaaccg
    16441 gtatggtggt ttcgaacgat ttgccgaaga actatcgcag gaacttaaca aaaggggtta
    16501 cacggtctat gtaaccagtc ctgcaactca ctcctgtaag gaactcaatg ccaataacat
    16561 ttttacggtt aacttaaggg taccaagatt tctcacatca aacctggcta cactttatta
    16621 cgattacatc tcccttaaat gggcaataag gaataacatt gatgcagtta ttgaatgcgg
    16681 gcactcgttt gccccgttgc tgctttttat atcggggtca atacggagga aaataattac
    16741 taaccccgat gggattgagc ataagcgtaa gaaatggggc tggattgcta aatgctacct
    16801 gcgcttttcg gagtatctag cctttaagtt atcggcacaa cttgtatgcg acaacagggc
    16861 attagtccag tactactcaa ccaggtataa aagaaggatt cactacattc cgtacggtgc
    16921 tcatcccttg atgcaagccc ctggcagacc agatgtgtta gacaaattgc atatcaacga
    16981 atattacctg ctaattacaa gaattacccc tgaaaacaac gttgaaacag tattaagtta
    17041 ctttgcccat agcgaccaat catgcctggt tgttggcgat ttaacatcaa gctatgcaaa
    17101 aaaaatagtt gggaaataca gtcattttag aaacattcat ctcttgggtg ccatttatga
    17161 ccagcaaatc ttaaatggct tgagatttta ctgtaaagca tacatccatg ggcattcggc
    17221 tggtggcact aacccttcgt tgcttgaggc tatggcttgt ggctgtttta ttattgcaca
    17281 cgataatcct tttaatcgtg atattttggg agagcaggga ctatatttca acaacatagc
    17341 tacactgggg agttgcattt cccaatttga aaaactaaac gatgttgagg taaatagggt
    17401 aaaagtgcaa aacattcaag ccattgagcg caatttctta tgggagaagg ttgctttggc
    17461 ttatatagat gttatagata tgttgaaaaa gtaaggggct acattttcag tagccccttt
    17521 tcttttattc gagaattaat tttcggatca gatacttctt tgagttgcca aacctgatgt
    17581 agtatagccc ttttggaagc ctctccatat tatcggtaat atcagtttca gtgttttcaa
    17641 tggtaatatc tctggatctc actttatttc ctattgcgtt gtaaaactcc agtttatacc
    17701 ttccgggttc aaatcccgta atcttaatgc tgaaattacc agttgtggg
//
LOCUS       DAOOWJ010000056        17330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3302_length_17330_cov_8.460839, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000056 DAOOWJ010000000
VERSION     DAOOWJ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 17330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17330
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3302_length_17330_cov_8.460839"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..2517
                     /locus_tag="PLW22_06910"
     CDS             217..2517
                     /locus_tag="PLW22_06910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOU74471.1"
                     /translation="MGKYCSTLTITLLFSTCAIFAQPKNYIKWVNPFIGTERMGHTFP
                     GATVPFGAVQLSPDTDTIPYSSNGKYNPDVYKYCAGYQYGDSTIIGFSHTHFSGTGHS
                     DLGDILVMPTVGKLYLNPGTASNTNTGYRSKFSHSNEFAEPNYYRVKLLDYNILAELT
                     TTTRVGLHRYTFPETDSAHIIIDLVHGIYNYPEKNVWTFIRVENDTLITGYRQTSGWA
                     RTRTVYFAITFSKPFKTYGIINGDKLIYKGFWRKFNQNTNFPENAGREIRCHFDFSTA
                     NHEQVLLKVALSPVSTSGAVENLNAEAPGWDFDAIKKEGQEQWNDELGKIEVETLTDS
                     DKVNFYTSMYHAFLSPTIYMDTDRKYRGLDQNIYTATNFDNYTTFSLWDTYRALHPLF
                     NIIQPKRNVDMVKSMLAHYSQSVHRMLPIWSHYANENWCMIGYHAVSVIADAAVKGND
                     GFDLPYALMACVQSAKNNWYDGLGYYREFGFVPEDKSGSSVSKTLEYAYDDWCIAQLA
                     KKLDREDIYNEFIRRSYNFQNVFDKSIGFMRPRLSNGSFKTSFNVLSTHGQGFIEGNS
                     WNYSLYVPHNPDALVSMLGGKKQLRAYLDTLFTMHLPDEYFAETEDITRDGLIGNYVH
                     GNEPSHHVPYLYNWTDNPWKTQYWVRTICNLMYQPKPDGLSGNDDCGQMSAWYIFSAL
                     GFYPVAPASPNYAIGSPLVTKATIKLENGKTLTISTQNQAKNNVYVQKVLLNGKPYNS
                     YFLNHNDIVSGGEITFIMGDKPKKLK"
     gene            2716..2907
                     /locus_tag="PLW22_06915"
     CDS             2716..2907
                     /locus_tag="PLW22_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOU74472.1"
                     /translation="MNKGTVKFFNESKGFGFIKDSETGEEYFVHATGLKDQISENDEV
                     TFNLEQGRKGLNAVDVKLT"
     gene            3005..4147
                     /locus_tag="PLW22_06920"
     CDS             3005..4147
                     /locus_tag="PLW22_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU74473.1"
                     /translation="MKIPQIAIFLSIVLSVYALVNLFIFYQTRPVFSLASIGTWLKFV
                     FWFIVLSYPLGRTLEAILGGQIPTLLVKVGSIWLAFMLYLTLFFLLFQITSPLINWLV
                     KIDIKSSLETHRMIVGMVYFATLTVILAGYINAVNPKINQLKIESSKPVPGGGLTIAM
                     VSDIHLGTIIGKNELSSLVDKINSQKPDIVLFVGDIFDEDIAPVVNGQMGEQFERINA
                     KYGVFAVTGNHEFFSNHQAIIEYLQKHGVNVLNDTVAEVANINIVGRYDRQSNFALGQ
                     SRKTLSELTKDIDQSKFTIVLDHQPFNLNESAESGADLQLSGHTHHGQMWPLNYITRA
                     VYEVSMGYKKIGNTHIYVSPGYGTWGPRVRLGNRPEIAVIRVKDKG"
     gene            4757..6469
                     /locus_tag="PLW22_06925"
     CDS             4757..6469
                     /locus_tag="PLW22_06925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOU74474.1"
                     /translation="MENFIVSARKYRPATFKSVVGQASITTTLKNAIQRKQLAQAYLF
                     CGPRGVGKTTCARIFAKTINCQNLSPELEACNECESCKSFNTNRSYNIHELDAASNNS
                     VEDIRKLIEQVRIPPQIGKYSVYIIDEVHMLSQSAFNAFLKTLEEPPAHAIFIMATTE
                     KHKILPTILSRCQIFDFNRIRVEDTVNYLEYIAKKEGVEYEVEALNIIAQKADGAMRD
                     ALSIFDQVVSFSENHITYQKVIENLNVLDYQYYFRLTDNFLKGDYADSLQIFDELLSK
                     GFDAQQFITGLSMHLRNVLVCHDPKTIPLLEVGAAIAQKYKEMAATCPPGFLIEALQV
                     TNQFDVAFRQSPNQRLHVELMLIKLCGLNQKKKSDDSQVEDSDPVEYSQPISSTVKPT
                     STPKAIPTTPKTPIRTTSIKGALNGAPNNAEIKNNDEPKVEEIQTENQGIASVEIKKI
                     SHEQILQAWTSFAEQIQKDKPAHFSLMQVYKPILKENNKITIQFEGQIQIELFSEIKK
                     DLLTYIKKSFGILNIEIAEEIVEKIEDNGKPRLYTPDDKFRFMAERNPALIRLKQQLN
                     LDLD"
     gene            6526..7038
                     /locus_tag="PLW22_06930"
     CDS             6526..7038
                     /locus_tag="PLW22_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOU74475.1"
                     /translation="MNKRLEQAINEQINAEIWSAYLYLSMSAHFGAQGFNGFANWFKV
                     QWQEELTHAMKFFDYLIERGSKPELKPIKEVPTKWDSPLHAFEETLKHEKEVTRLINN
                     LMDIAIEEKDHAAKGMLQWFVDEQVEEEANAQELIDTLKLINGNGNGLFMLDRELKQR
                     AFVGDTKKAE"
     gene            7287..10568
                     /gene="secA"
                     /locus_tag="PLW22_06935"
     CDS             7287..10568
                     /gene="secA"
                     /locus_tag="PLW22_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOU74476.1"
                     /translation="MGLSNFLTKFFGTKSDRDLRELMPIVNKIREAYPRYTTLTNDQL
                     REETDRLRKVVRDYIAEDEAKVEQLKAQMESDEIDYEEKERVYKEVERLTKQIDDKIE
                     EVLNQVLPDAFSIVKETARRFKENEIIEVTATQFDRDLAARKDFVTIQGDKAIYKNRW
                     MAGGNEIVWDMVHYDVQLIGGVVLHQGKIAEMATGEGKTLVATLPVFLNALAGKGVHV
                     VTVNDYLARRDSEWMGPIYEFHGLSVDCIDKHQPNSEARRRAYNCDITYGTNNEFGFD
                     YLRDNMAIAPDDLVQRKHHYAIVDEVDSVLIDDARTPLIISGPVPKGEDQMFEEYKPK
                     VEKLVNAQRSLITQLLTDARKLISEGNTEEGGKLLLRAHKGWPKYKPLIKFLSEQGNK
                     ALLLKTENFYMQDNNRNMHLITDDLYFVIDEKQNSVELTDKGIDLITSSAEDPKFFVL
                     PDIGSEIAEIEKMSITQGEKLTKKDELLREYAVKSERVHTVHQLLKAYTMFEKDVEYV
                     VMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKERVKVEAATQTFATITLQNYFRMYH
                     KLAGMTGTAETEAGEFWSIYKLDVVVIPTNKPVVRKDMEDLVYKTKREKYNAVIDEIV
                     RLRDANRPVLVGTTSVEVSELLSRMLKLKGIQHNVLNAKQHQREAEIVAHAGIPKTVT
                     IATNMAGRGTDIKLTPESRAAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGTSQFYV
                     SLEDDLMRLFGSDRIARVMDAMGHKEGDVIQHSMVSKSIERAQKKVEENNFGIRKRLL
                     EYDDVMNAQREVIYKRRRHALFGERLDVDISNMFMDVCEAIVNEFHGNYEYDDFKLEI
                     IKQFSLDVPITEDEYLELKPSEIAHRLYKVALESYDRKAHEIARQAYPVIKEVYEKQS
                     AIYENIVVPISDGQKVYQTVTNLKKAYETGGKELIRSFTKNIILFTIDEYWKEHLREM
                     DELKQSVQNAVYEQKDPLLIYKLEAFDLFRTMIDKLNRDIVAVLVKAFIPLRDPNQVQ
                     EARQSRRTDMSQYQTSRTDNLQPGANTQEQSRQPVKVEVKVGRNDPCPCGSGKKYKNC
                     HGKNL"
     gene            10592..12382
                     /gene="argS"
                     /locus_tag="PLW22_06940"
     CDS             10592..12382
                     /gene="argS"
                     /locus_tag="PLW22_06940"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU74477.1"
                     /translation="MTAEKFIQVLVKSAAEKLYNQPIADSLIQIQKTRKEFEGDYTMV
                     AFPLLKLSRKSPEETCKEIGEQLVALDKHIESFNVIKGFLNITLSKSYWANVLNTIVQ
                     TNDFGFCEAKSDKPIVIEFSSPNTNKPLHLGHIRNNLLGMSISRIMEKSGKRVVKVNL
                     VNDRGIHICKSMLAWQKFGNGETPELTGVKGDHLVGDYYVRFDKEYKAQVEELKAKGM
                     PEDEAKLKAPIIQEAQEMLRRWEAKDPEVIHLWKTMNGWVYEGFDKTYADLGITFDKV
                     YHESETYLLGKSVVMDGLNRGIFFRKDDGSVWVDLTNDGLDQKLLLRGDGTSVYITQD
                     IGTAIQRYNEFNFDEHIYVVGNEQEYHFQVLKLIMKKLGYEWSDALYHLSYGMVQLPE
                     GKMKSREGTVVDADDLIEEMIETARQMSEELGKLDDVDHGVASEVIRMVGLAALKYFI
                     LKVDPKKNMTFNPKESIDFNGNTGPFIQYTHARIKSVLAKATHAGYQVNGIIQPDFIS
                     NSKELELIQLLSQFPEIVLAAAQNHNPSLIANYAYELAKEYNQFYHDFTILKEPNEGI
                     RLLRLSLSEQVADIIKRAMWLLGIDVPERM"
     gene            12443..13051
                     /locus_tag="PLW22_06945"
     CDS             12443..13051
                     /locus_tag="PLW22_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74478.1"
                     /translation="MVTTFNVKAQEFEPAKDFGSEFGITGMFGKEGIDGNNSAYVGFT
                     GAYFYDLKNGLRVGINRYYNFSGAEYAYTLPIYFAHRTKSSRSNEPFVFDTFGEFLLQ
                     LFTHIIPGRAEFNAGPVIGYFKSTNQTISNGYKLTNPVYLAANAKLRLTFQIWRFSLG
                     GNFGASYVPSKNFYLVSPDPVFNGNRTSWSLEIGAFLSFSFD"
     gene            13107..>14398
                     /gene="ffh"
                     /locus_tag="PLW22_06950"
     CDS             13107..>14398
                     /gene="ffh"
                     /locus_tag="PLW22_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOU74479.1"
                     /translation="MFENLTDRLERSFKILKGEGKITEINVAETVKEIRKALIEADVN
                     YKVAKTFTDEVKRKALGMNVLNAIKPGQLMVKVVHDELVSLMGGQATDINLAGNPTVI
                     LIAGLQGSGKTTFSAKLANHLKNKKGKSPLLVAGDVYRPAAIEQLKVLGQQINVPVFT
                     IPGSNDPIGIAREAVRLAKNNNQNVVIIDTAGRLAIDEEMMKEVEAIKKAVSPNEILF
                     VVDSMTGQDAVNTAKEFNDRLNFDGVVLTKLDGDTRGGAALSIRAVVDKPIKFVSTGE
                     KLDALDIFHPDRMADRILGMGDIVSLVEKAQEQYDAEQAKILHKKILKDQFNFNDFLE
                     QINQIKKMGNLKDLAGMIPGVGKALKDIDIDDDAFKNIEAIIKSMTPEERENPSLMNG
                     SRRKRIAEGSGTSIVEVNRLLKQFEDMRKLMKMVSGGGA"
     assembly_gap    14399..14498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14569..15450
                     /gene="folD"
                     /locus_tag="PLW22_06955"
     CDS             14569..15450
                     /gene="folD"
                     /locus_tag="PLW22_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOU74480.1"
                     /translation="MELLDGKRIADEIKAEIAAEVEAIKGKGGKIPHLVAILVGHDGA
                     SETYVGHKEKACAQVGFKSQVLRFEDSITEAQLLDEIRKLNNDPDVDGFIVQMPLPKH
                     IDEQKVIEAIDPKKDVDGFHPVNVGRMNIGLPAYISATPYGIVELLRRYKIPTEGKHC
                     VVIGRSNIVGRPIANLLSQKSYPGDCTVTICHSRTPNIKEYTLQADILIVALGKAEFV
                     TADMVKPGAVVVDVGITRVDAPGTKLGWKLLGDVKFDEVAPKCSYITPVPGGVGPLTI
                     VSLLKNTLKAAKKEVYG"
     gene            15461..16168
                     /locus_tag="PLW22_06960"
     CDS             15461..16168
                     /locus_tag="PLW22_06960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU74481.1"
                     /translation="MNNQETTAKFYDEFSDKQVKTGKNLRHYTLANILKKKGIGKANS
                     VLEIGCGIGTLTDLLVSIAPKAVITAFDISPKNIEIAKGRIKSSRVKFMVSDASESIS
                     LNEKYDFVILADVIEHIPIERYNSLFSNLKQFSHTGTKIFINIPHPNLIKYLRETKPE
                     SLQIIYQSVYPTILYQHLSEQNFVIDEKVDYSIFYKTPDYTYYWLSLFSEKSNSNYSK
                     TVPLPFRIFKKFILRVI"
     gene            16170..17228
                     /locus_tag="PLW22_06965"
     CDS             16170..17228
                     /locus_tag="PLW22_06965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU74482.1"
                     /translation="MNILYISSGNPTFVRKDILLLSKQNNVKSLLYTWNTVNTPINFI
                     RQFIYLIPRLKKIDVIIVMFAGYWSVIPALFGKIFNKKVFIILGGTDCVSYPEFNYGS
                     LRKPLLKSAIYYSIKNATCLLPVHESLIDFTNSYYDKSKQGIKNHFKSIKTDIKTVYN
                     GYNPRVSHDTIEKSIRKPNSFITVALVNNKIRLILKGIDLILDSAREFPNASFTIIGI
                     AETIKSQLEIPSNVIIHPPMSSELLAEKYLKSEFVIQPSISEGFPNALCEAMSFGCIP
                     IVSPVGAMPTITENIGFTVKNRSTEELSSVIKNALNLTNDEKVNLRFLSHSKIVNNFS
                     EKERISKLEQIIEEYKYK"
BASE COUNT         5510 a         3500 c         3583 g         4637 t
ORIGIN      
        1 gcgggggttc taaatatcag ataatcaaac tcgtttgaaa ataggctgtc cgacaaattt
       61 aatttccgta tttttgccca agttgtatgt ttagttcagt aaaacaaaac tacaacatgg
      121 ggcgaaacct tcggtcggta accccttatt ttatttttta cccgttcagt agtgaaataa
      181 atatttattt taactgaaat tttagccagt tagaaaatgg gaaagtattg ctctacacta
      241 acaattacct tacttttttc aacttgtgca atttttgcac aacctaagaa ttatatcaaa
      301 tgggtaaacc cattcattgg aactgagcga atggggcata ctttcccagg cgcaaccgtt
      361 ccgtttgggg cagtgcaact tagtcccgat accgatacta tcccctactc ttccaatggc
      421 aagtacaatc ccgatgttta caagtattgt gctggttatc agtacgggga tagtaccata
      481 attggcttta gccataccca ttttagcgga acagggcatt ccgaccttgg tgatattctt
      541 gtgatgccaa cggttggcaa actttactta aaccccggaa ctgcttccaa tacaaatact
      601 gggtatcgct ccaaattttc tcattccaat gagtttgccg aaccaaacta ctacagggtc
      661 aaactcttag attataacat tcttgcagag ctaactacaa ccacaagggt tgggctacat
      721 cggtacacat tccctgaaac agactcagca cacataatca ttgaccttgt tcatggaatt
      781 tataattatc ccgaaaaaaa tgtttggaca ttcattcggg ttgaaaacga tacccttata
      841 accgggtacc gacaaacatc gggatgggca cgcacccgca ctgtatactt tgccattacc
      901 ttttcaaaac catttaaaac ctatggcata atcaatggcg ataagttgat ttataaaggt
      961 ttttggcgaa aattcaatca aaacaccaat ttccctgaaa atgctgggcg tgaaattcgt
     1021 tgccacttcg acttttcaac tgcaaaccat gaacaagtat tgctaaaagt tgcattatcg
     1081 cccgtgagta ccagcggagc agttgaaaat ctaaatgccg aagcaccagg ctgggatttt
     1141 gatgccatca aaaaagaggg tcaagagcaa tggaatgatg agctaggtaa aattgaagtt
     1201 gaaaccctta ctgatagtga caaggtaaac ttttacacat cgatgtacca tgcctttcta
     1261 agtccaacca tttacatgga tactgatagg aaatatcgag ggcttgacca aaacatttac
     1321 acagcaacca attttgataa ctacactacc ttctcactct gggacacata tagagctttg
     1381 catccgcttt ttaatattat acagccaaaa cgaaatgtcg atatggtaaa atcgatgttg
     1441 gctcattaca gtcaaagcgt tcatcgcatg ctccctatat ggtcgcacta cgcaaatgag
     1501 aactggtgca tgattggata ccatgccgta tcagtgattg ccgacgcagc cgttaaaggc
     1561 aacgatggct ttgatttacc ctatgcgctt atggcttgcg tgcaaagcgc taagaataac
     1621 tggtacgatg ggttaggcta ttacagagaa tttggttttg ttcctgagga taaatcgggg
     1681 agttctgttt ccaaaaccct tgaatacgct tacgacgatt ggtgcattgc acaacttgca
     1741 aaaaaactgg atagagagga tatttacaac gagttcataa gacgttcata caactttcaa
     1801 aacgtattcg ataagtccat tgggtttatg cgtcctcgtt tatcaaacgg gagtttcaaa
     1861 actagtttta acgtgcttag cacacatgga caagggttta tagagggtaa ctcatggaac
     1921 tatagccttt atgtacccca taaccccgat gctttggtaa gcatgcttgg tgggaaaaag
     1981 caactccgag cctatcttga cactctcttt accatgcatc ttccggatga atacttcgcc
     2041 gaaaccgaag acataacccg cgatggattg ataggcaatt acgttcatgg gaatgaacca
     2101 tcgcaccatg ttccatatct ttacaactgg actgataacc cttggaaaac gcagtactgg
     2161 gttagaacca tttgcaacct aatgtaccaa cccaaacctg acggattaag cggaaatgat
     2221 gattgcggac agatgagtgc ctggtacatt tttagcgccc ttggtttcta tcctgtagca
     2281 cctgcatcgc ccaactatgc tattggcagc ccattggtta ccaaagccac aattaaactg
     2341 gaaaacggaa aaacacttac catttcaacc caaaatcaag ctaaaaacaa tgtttatgtt
     2401 cagaaggtgt tgcttaatgg caaaccttac aatagttact ttttaaacca taacgatata
     2461 gtaagtggtg gtgaaataac atttattatg ggtgataaac ccaaaaagtt aaagtgaaaa
     2521 ggaataagca caaagcatca atggaactaa tacctcgccg aatcgaaata tttttgaaac
     2581 aattaacaca attaaacaaa tgttatctat ttgacataca attacataag aaatatattt
     2641 aaaataaatc taaattaatg gtgctaaatc caaatattat gcgttacttg cacccgaatt
     2701 aataaatcac aacaaatgaa caaaggaaca gtaaaatttt ttaatgaatc aaaaggcttt
     2761 ggttttatta aagattcaga aacaggtgaa gagtattttg tacacgccac tggattaaaa
     2821 gatcaaattt cagaaaacga tgaggttact ttcaaccttg aacaaggtag aaaagggctg
     2881 aatgcagtag acgtaaaatt gacttagatt atccaacata cttgtttaaa tcccggtttt
     2941 accgggattt ttttttacca tgaactttca aatgctaatt ttgttaattg aaaaaaatga
     3001 aaacatgaaa attcctcaaa tagccatttt tctatcaatt gttctaagcg tttacgcgct
     3061 tgttaattta ttcatatttt accaaacccg accggtattc agcttagctt caattggaac
     3121 ttggttaaag tttgtatttt ggtttattgt actctcctac cctttaggta gaacccttga
     3181 agctatatta ggtggacaaa ttccaacact tcttgtaaag gttggctcaa tttggttagc
     3241 ctttatgctt tacctaacac tttttttcct actttttcaa ataacatcgc ctcttataaa
     3301 ttggttagta aaaatagata ttaaaagctc cctcgaaaca catagaatga tagttggaat
     3361 ggtatacttt gcaacgctta cagtcattct tgctggctat ataaacgctg tcaaccccaa
     3421 gataaaccag ctaaaaattg aatcaagtaa acctgttcca ggtggaggct taaccatagc
     3481 aatggtttcc gatattcacc tgggaacaat aattgggaaa aatgaattaa gcagtcttgt
     3541 ggacaaaatc aactcacaaa agcctgatat tgtcttattt gtaggtgata tttttgatga
     3601 ggacattgcc ccggtggtaa atggtcaaat gggagagcaa tttgagcgta ttaatgcaaa
     3661 atacggcgtt tttgcagtaa ccggaaacca tgaattcttt agtaaccatc aggcaataat
     3721 tgagtacctt caaaaacatg gtgtaaacgt tttgaacgac acggttgctg aagttgccaa
     3781 catcaacatt gtaggccgtt acgaccggca aagcaatttt gccttggggc aaagccgtaa
     3841 aactttatcg gaattaacaa aagatatcga ccagagtaag tttacaatag tgctcgacca
     3901 tcagccattc aatctaaacg aatccgctga atccggagct gacttgcagc tttcgggtca
     3961 cacccatcat gggcagatgt ggcctttaaa ctacattacc cgagcggtat atgaggttag
     4021 catgggatat aaaaagattg gcaatacaca catatatgta tcgcccggct atggaacctg
     4081 gggcccaagg gtaaggcttg ggaatagacc ggagatagcg gtgataaggg taaaggataa
     4141 aggataaagg ataaaggata aaggttaaag gataagttga tggttgatgg ttgatggttg
     4201 atagtgaaaa aaagcattac actaaacact gaaaaccaaa tactactctc catattccct
     4261 gtaaactgca cactgtacac tgcacactat tcaaaagatc tttcgctacg ctcgggatga
     4321 caaatgaagt tgttcgtgta tagttgctcg ttgttcgtga aactattgtc atgctgaacc
     4381 cagtcgaagt atcgctttgc tcttttgccc ttctgtcggg tttgagatcc ttcgctacgc
     4441 tcaggatgac aagctagtta atgattgaaa gttgattgtg ctgcaacggg atataaaacc
     4501 ttacactgtg taactttgtg ttaccccgtt taacactgtg atactgtata tgcttgtagt
     4561 aagattcaag aaatcagaaa ggtagtttta tttcattttg agcatcccgt tccaccatag
     4621 ctgttttttt tattaattca aaaatccctg ttaataactt attaccctca cattctgtaa
     4681 attatagtat ttaacagtta atactccact gttagtttaa gtattttcct atttttgttt
     4741 ttgaaatttt ggcggaatgg agaatttcat tgtatcggca cgaaagtatc gcccagcaac
     4801 gtttaaaagc gtggttggac aggcatcaat tactactacc ctaaaaaacg ccattcagcg
     4861 taagcagtta gcccaagcct acttattctg cgggccacga ggggtaggca aaacaacatg
     4921 tgcccgaatt tttgccaaaa ccataaactg ccaaaacctt tcccctgagc tggaagcctg
     4981 taatgagtgc gaaagctgca aatcgtttaa caccaaccgc tcctacaata tccatgagct
     5041 cgatgcagca tcgaacaact ccgttgagga tatccgaaaa ttaattgagc aggttcgcat
     5101 accgccccaa attggtaagt atagcgtata cattatcgat gaggtgcaca tgctctcgca
     5161 aagcgcattt aatgctttcc ttaaaacact tgaggaaccg ccagcccatg ccatttttat
     5221 tatggcaacc accgaaaaac acaaaatcct ccctaccatt ctttcgcgtt gccagatatt
     5281 cgattttaac cgtatccgcg ttgaggacac cgtaaattac cttgagtata tcgctaaaaa
     5341 agagggtgtt gaatatgagg tagaagcctt gaatataatt gcccaaaagg ccgatggggc
     5401 catgcgcgat gccctttcaa tctttgacca ggtagtgagc tttagcgaga accatattac
     5461 ctaccaaaag gtaattgaaa atctgaatgt tctcgactac cagtattact tccgtttaac
     5521 cgataatttc cttaagggcg attatgctga ctcattgcag atattcgatg agcttctctc
     5581 aaagggtttc gatgcccaac agtttataac tgggctaagc atgcatctaa ggaatgtgct
     5641 tgtttgccac gatccaaaaa caattccctt actcgaggtt ggagcagcta ttgcccaaaa
     5701 gtataaggag atggcagcaa cctgcccacc aggtttctta attgaagcgc ttcaggtcac
     5761 caaccagttt gatgttgcct ttaggcaaag ccccaaccaa aggcttcatg ttgagctgat
     5821 gcttattaag ctttgtgggc ttaatcaaaa aaaaaaatct gacgacagcc aggtagagga
     5881 ctccgatccc gttgagtaca gtcagcctat atcatctacc gtaaaaccaa catctacgcc
     5941 aaaagcaata ccaacaaccc ctaaaacacc tatccgtacc acatcaatca aaggcgcatt
     6001 aaacggagca ccgaataatg ctgaaattaa aaataatgat gaacccaagg tagaggaaat
     6061 ccaaactgaa aaccaaggga ttgcaagtgt tgaaattaaa aaaatttcgc acgaacaaat
     6121 actacaggct tggacaagct ttgctgaaca aattcagaaa gataaacccg cacattttag
     6181 cttaatgcag gtgtataagc caattttaaa agaaaataat aaaatcacta ttcagtttga
     6241 gggtcaaatc cagattgaac tttttagcga gataaaaaaa gatttgctca cctacatcaa
     6301 gaaaagtttt ggaatcttaa acattgaaat agctgaggaa attgtagaaa aaattgaaga
     6361 taacggaaaa ccaaggctct acactcccga cgataagttc cgctttatgg cagaacggaa
     6421 tcctgcacta atccgtttaa agcagcaatt gaaccttgac ctcgattaat tgttatttaa
     6481 gtagcaatta actatatttg aaataaaacc taaaaaacta aagctatgaa taaacgttta
     6541 gaacaagcta tcaatgagca aataaatgcc gagatttggt cggcatacct ttacctgtcc
     6601 atgtcagctc actttggagc gcagggcttc aatggctttg ccaattggtt taaagtacag
     6661 tggcaggagg agctaaccca tgccatgaaa tttttcgact acctaattga gcgcggcagt
     6721 aagcctgagc tcaagcccat taaggaggtt ccaaccaaat gggattcccc tctccacgcc
     6781 tttgaggaga cactaaaaca tgaaaaggag gttacccgct taatcaataa ccttatggat
     6841 attgccattg aggaaaaaga ccatgctgca aaaggaatgc tgcaatggtt tgtagatgaa
     6901 caggttgaag aggaagccaa tgcccaggaa ttaattgata cgctaaagct aatcaacggc
     6961 aatggcaatg gacttttcat gctcgaccgt gagcttaaac aacgcgcttt tgtaggcgac
     7021 accaaaaagg cagaataaaa aatatcgcca aagagaaacg gggctaatta gccccgtttt
     7081 ttaattgcta aagatttgac tattaaaaga gtatacaaaa ggtattaatt agaaaaccta
     7141 ccaatcctat aataattaat gctatagtaa taggcattta aaaatttagt attaacagaa
     7201 ttaaactctt attcggtagt atcctttcgt tatctaaaaa attcaagtaa atttgtaagc
     7261 ttcaaaaaat cttaaatttt tacaaaatgg gcttaagtaa ttttctaacc aaattttttg
     7321 gaaccaaatc ggatcgcgat ttacgtgaac tgatgcctat agtaaacaaa attagggagg
     7381 cttacccccg atataccacc ttaacaaacg atcagctaag ggaagagact gataggctac
     7441 gcaaggtagt gcgcgattat atagctgagg atgaggctaa agtggagcag cttaaagccc
     7501 agatggagtc ggatgagatt gactatgaag aaaaagaaag ggtttacaag gaagttgagc
     7561 gattaacaaa gcagattgac gataaaattg aggaggtgct taaccaggta cttcccgatg
     7621 ccttttccat tgtcaaggaa accgcacgta ggttcaagga gaacgaaatc attgaggtaa
     7681 cagccacaca gtttgaccgt gaccttgccg cccgaaagga ttttgttacc atacaaggcg
     7741 ataaggccat ttacaaaaac cgctggatgg ccggaggtaa tgaaattgtg tgggatatgg
     7801 ttcactacga cgtacagctc attggtggcg ttgtactgca tcaggggaaa attgccgaaa
     7861 tggcaacagg tgaaggtaaa acgctggttg ccacactacc ggtgttcctg aatgcccttg
     7921 ccggcaaagg cgtacatgtg gttaccgtaa acgattatct ggctcgccgc gactcggagt
     7981 ggatggggcc aatatatgag ttccacggac tttcagtgga ctgtatcgat aagcatcaac
     8041 ccaactctga ggctcgccgt agagcttaca actgcgatat cacctacgga accaacaatg
     8101 agtttggttt cgattacttg cgcgataaca tggcaattgc tcccgatgat ctggttcaac
     8161 gaaagcacca ctatgccatt gtggacgagg ttgactcggt tttaattgac gatgctcgaa
     8221 ccccgctaat catctcaggt cccgttccaa aaggcgagga tcaaatgttt gaggagtaca
     8281 agccaaaggt tgagaaactt gttaatgccc agcgttctct tattacccaa cttttaactg
     8341 atgctcgtaa acttatatcc gaagggaata ccgaggaggg aggaaaacta ctactccgcg
     8401 ctcacaaggg ttggccaaaa tacaaacccc ttatcaaatt cctaagcgaa caaggtaata
     8461 aggcactcct gcttaagact gaaaactttt acatgcagga taacaaccgt aacatgcacc
     8521 ttattactga cgatttatac tttgtaattg acgaaaagca aaactctgtt gagctaaccg
     8581 ataaggggat tgacctcatc accagttcag ccgaagatcc gaagtttttc gttctacccg
     8641 acataggaag cgaaatcgcc gaaatcgaga agatgtcgat aacacagggc gaaaagctaa
     8701 ccaaaaagga tgagttactt agggaatatg ccgtaaaatc ggagcgcgta catactgttc
     8761 atcagctact aaaggcctac accatgttcg agaaggatgt ggagtatgtt gtaatggata
     8821 acaaggtaaa aattgttgac gagcaaacag gtcgtatcct ggaggggcgt aggtattccg
     8881 atggtcttca ccaggccatt gaggccaagg agcgcgttaa ggttgaggct gccactcaaa
     8941 cgtttgccac aattacactt cagaactact tccgtatgta ccataagctg gcaggtatga
     9001 ccggtactgc tgaaactgag gctggtgagt tctggagtat ctataaactt gatgtggtag
     9061 ttatccccac aaacaaaccg gttgtccgca aggatatgga agatttggtt tacaaaacca
     9121 aacgcgagaa gtacaatgca gtaattgatg agatagttag actccgcgat gcaaaccgtc
     9181 cggtactggt tggtactacc tcggttgagg tgtcggaatt gctgagccga atgctaaagc
     9241 ttaaaggaat acagcataat gtgcttaacg ccaagcaaca ccaacgcgag gcagaaattg
     9301 tggcacatgc aggtataccc aaaacggtaa ccattgccac caacatggca ggtcgtggta
     9361 ctgacattaa gctaacccct gaatcgcgtg ctgcgggcgg tttggctatt attggtactg
     9421 agcgccatga atccaggcgt gttgaccgcc agctgcgtgg tcgttcggga cgccagggcg
     9481 atccgggaac atcgcaattc tatgtttcac tggaggatga tttaatgcgc ctttttggct
     9541 cggaccgcat cgcaagggta atggatgcca tggggcataa ggagggcgat gtaattcagc
     9601 actcaatggt atcaaaatcc attgaacgcg cacagaaaaa ggttgaggag aacaacttcg
     9661 gaatccgtaa gcgtttgctg gagtacgacg acgtaatgaa tgctcagcgc gaggttattt
     9721 acaagcgtcg ccgccatgca ctctttggcg aaaggctcga tgtggatatc tcaaatatgt
     9781 tcatggatgt ttgcgaggca atagttaacg agttccatgg taattacgag tacgatgatt
     9841 tcaagctaga gatcatcaag caattttcac tcgatgtccc tataaccgaa gacgagtacc
     9901 ttgagcttaa accatcagag atagcccata ggctttacaa ggttgcccta gaatcgtacg
     9961 atcgaaaggc gcatgaaatt gcacgacagg cctacccggt aatcaaagag gtttacgaaa
    10021 aacaatcggc catctacgag aatatcgtgg taccaatatc cgacggtcaa aaggtttatc
    10081 aaaccgttac aaatctgaaa aaggcttacg agacaggtgg taaggaacta attcgttctt
    10141 tcacaaagaa catcatactg ttcactattg atgagtactg gaaggaacac ctgcgtgaaa
    10201 tggacgaact aaaacagagc gttcagaatg ccgtgtacga gcaaaaagac ccactgctca
    10261 tctataagct ggaagcattt gaccttttcc gcacaatgat tgataagctt aaccgcgata
    10321 ttgttgcggt tcttgtgaaa gcgttcattc cgcttcgcga tcccaaccag gtacaggaag
    10381 cccgtcaaag ccgacgtacc gatatgagcc agtaccaaac cagccgtacc gataatttac
    10441 agcctggcgc aaacacccag gagcagagcc gtcaaccagt aaaagttgaa gttaaggtag
    10501 gacgtaacga tccatgccct tgcggtagcg gtaaaaagta caagaattgt cacggtaaaa
    10561 acctataatt atttctgaac caaaaggaaa aatgacagca gagaagttca ttcaggtatt
    10621 ggtaaaatcg gcagctgaaa aactatacaa ccaaccaatt gccgatagtt taatacagat
    10681 acaaaaaaca agaaaggaat ttgaaggcga ctacaccatg gtcgcctttc cgctgcttaa
    10741 gttgtcgcgt aaatcgcccg aggaaacctg taaggaaatt ggcgaacaac ttgtagcatt
    10801 agataaacat attgagagtt tcaatgtaat caaaggtttc ctgaacataa ccctttcaaa
    10861 atcgtactgg gctaatgtac tgaataccat tgttcaaacc aacgattttg ggttttgtga
    10921 ggccaaatcg gataaaccga tagttattga attctcatcg cccaacacaa ataaaccctt
    10981 gcaccttgga catatcagga ataaccttct tggaatgtca atttcccgaa tcatggagaa
    11041 atcaggaaaa cgggtagtta aagtaaacct ggtcaacgat cgtggcatcc atatctgcaa
    11101 atcgatgctt gcctggcaaa aatttggcaa tggcgaaaca cctgaattaa ctggagtaaa
    11161 gggcgatcac ctggtgggcg attactacgt tagattcgat aaggagtaca aggcccaggt
    11221 tgaggagcta aaagccaagg ggatgcccga ggatgaggca aaactaaaag ccccaattat
    11281 tcaggaggca caggaaatgc ttcgacgctg ggaagccaag gatccagagg ttattcacct
    11341 atggaaaacc atgaatggtt gggtttatga gggatttgat aaaacttacg ccgatttagg
    11401 aattacattc gacaaggttt accacgagtc cgagacttac ctactcggaa aatcggtggt
    11461 tatggatggg ttaaaccgag gaatattctt caggaaggat gatggttcag tgtgggttga
    11521 tcttacaaat gacggattgg accagaaatt gcttttacgt ggcgatggaa cgtcggtata
    11581 catcacacag gatattggca cagccattca acgatacaat gagttcaact ttgatgaaca
    11641 catttacgtt gttggaaacg agcaggagta tcactttcag gtgttaaagc tgataatgaa
    11701 aaagttggga tatgaatgga gcgatgctct ttaccacctt tcctatggta tggttcagct
    11761 tcctgaaggt aaaatgaaat cgcgcgaggg cacagtagtc gatgccgacg atctgattga
    11821 agaaatgatt gaaactgcac gccaaatgtc agaggagtta ggcaaactcg atgacgttga
    11881 ccatggagtg gcatctgaag ttatcagaat ggtaggcctt gctgcgctta agtacttcat
    11941 cttgaaggta gatcccaaga aaaacatgac tttcaaccca aaggaatcca tcgactttaa
    12001 cgggaatact ggcccattca tccagtacac ccatgctcga attaagtcag ttcttgccaa
    12061 ggcaacccat gcaggctatc aggttaatgg aataatacaa cccgacttca ttagcaattc
    12121 aaaggaactt gagcttatac agcttttaag ccaatttcct gagattgtcc ttgcagccgc
    12181 tcaaaaccat aatccttcat taattgccaa ttacgcttac gagctagcca aagaatataa
    12241 ccagttctac catgatttca ccattcttaa agaacctaac gagggtataa ggttgcttag
    12301 gttatcgctc tcggaacagg tagccgatat aatcaaacgg gccatgtggt tacttggtat
    12361 cgatgtgcct gaacgaatgt aatcccttga tttttttttt agtatgcaat tgaataagtt
    12421 ttgtctttct gcctttgtaa ttttggttac aacttttaac gttaaggcac aggaatttga
    12481 gcccgctaag gattttggct ctgagtttgg aataacaggc atgtttggga aggaaggaat
    12541 agatgggaac aactctgctt acgttggatt tacaggagct tacttctacg atttaaaaaa
    12601 cggacttcga gttggaataa atcggtacta caattttagc ggagccgaat acgcttatac
    12661 cctccctatc tactttgctc atcgaaccaa atccagtaga agtaatgaac cctttgtgtt
    12721 tgatactttt ggcgagttct tactacaact ttttacccat ataattcccg gccgtgcaga
    12781 gttcaatgct ggaccggtga ttggttattt taaatcaacc aaccaaacca ttagtaatgg
    12841 gtataaactc accaacccgg tttaccttgc agccaatgca aagcttaggc ttacctttca
    12901 gatttggcgg tttagtcttg ggggaaactt cggcgccagc tacgtaccat caaaaaattt
    12961 ttacttggtt tcacccgacc ccgtctttaa tgggaatagg accagttggt cgcttgagat
    13021 tggtgccttt ctatcattct ccttcgatta aaaaaaccat atttttatta agtttgcata
    13081 ataatttgaa ataagagcat accgatatgt ttgagaattt aaccgatagg cttgagcgct
    13141 cttttaaaat actaaaaggt gaaggtaaaa tcaccgagat taatgttgcc gaaaccgtaa
    13201 aggaaatccg caaggcactt attgaggccg atgttaacta caaggttgcc aaaacattta
    13261 ccgatgaggt aaagcgtaag gcacttggca tgaatgtact aaatgccatt aagcctgggc
    13321 aactgatggt taaggttgta cacgatgaac ttgttagcct tatgggcgga caagccacgg
    13381 atattaacct tgcaggtaat ccaacagtta tcctaattgc aggtttacag ggttcaggta
    13441 aaacaacttt ctcggcaaaa cttgccaacc acctaaaaaa caaaaagggg aaaagccctc
    13501 tgctggttgc aggcgacgtt taccgacccg ctgctattga acagcttaag gttttaggtc
    13561 aacaaattaa tgttccagta tttaccatac ccggcagcaa cgatcctatt ggaattgcca
    13621 gggaagctgt caggctggca aagaacaaca atcagaacgt tgtaataatt gatactgctg
    13681 ggcgtttggc tatcgacgag gagatgatga aagaggttga agccatcaaa aaggctgttt
    13741 cacccaatga aatccttttc gttgtcgact ccatgaccgg ccaggatgcg gtaaacaccg
    13801 caaaggaatt taacgatagg ctcaactttg acggcgttgt gcttaccaag ctcgatggtg
    13861 atacccgtgg cggtgcagcc ctctccatac gtgccgttgt tgataaaccc ataaaattcg
    13921 ttagcaccgg cgaaaaactt gatgctctcg atatcttcca ccccgaccga atggccgatc
    13981 gtatccttgg aatgggcgat attgtttcgc tggttgagaa agcgcaagaa caatacgatg
    14041 ccgaacaggc taagatcctc cacaaaaaga tacttaagga tcagttcaac tttaacgatt
    14101 ttcttgaaca gattaaccag ataaaaaaga tgggtaacct aaaagacctt gccggtatga
    14161 ttcctggagt tggcaaggcc ttaaaggata ttgatattga tgatgatgct tttaaaaaca
    14221 tcgaagccat catcaaatcg atgacccctg aagaacgcga aaacccatcg ctgatgaatg
    14281 gcagtcgccg taagcgtata gcggagggta gcggaacatc aatcgttgag gttaaccgat
    14341 tgctgaaaca atttgaggat atgcgtaagt taatgaaaat ggtatcgggc ggtggtgcnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gtttggtgtt tggatttagg
    14521 aattctaact tcaagtaaaa attcagttta atattaactc gaaaatcaat ggaactactc
    14581 gacggaaaaa gaatagccga tgagataaaa gctgagattg cagctgaagt agaggctatt
    14641 aaaggcaaag ggggcaaaat cccacacctg gttgccattc ttgtaggaca cgacggcgca
    14701 agcgaaacct atgttggcca taaagagaaa gcatgcgctc aggtgggttt caagtcgcaa
    14761 gttctccgtt ttgaggattc cataactgag gctcaacttt tggacgagat taggaaactg
    14821 aataacgacc ccgatgttga tggtttcatt gttcaaatgc ctttaccaaa gcatatcgac
    14881 gagcaaaagg ttattgaagc aattgacccc aaaaaggatg tagatggatt tcaccctgtg
    14941 aacgttggga ggatgaacat tggcctgcct gcctatattt cagccacacc ctatggtatt
    15001 gttgaactgc tacgtcgcta caaaatcccc acagagggga aacactgcgt ggttataggc
    15061 cgtagcaaca ttgttggtcg tcccattgca aacctgcttt cgcagaaaag ttaccccgga
    15121 gattgcacag taaccatttg ccatagccga acgcccaata tcaaggaata tacgctccag
    15181 gccgatatac taattgtagc ccttggcaag gctgagtttg taaccgccga catggtaaaa
    15241 cctggagcag ttgtggttga tgtgggtatt acccgggttg atgcccctgg cactaaattg
    15301 ggctggaaat tgttaggcga cgtcaagttt gatgaagtgg cacccaaatg tagctacatt
    15361 accccggttc cgggaggtgt aggcccgcta acaattgtat cgctactaaa aaataccctg
    15421 aaagcagcca aaaaagaggt ttacggataa tgctactcaa atgaacaacc aggaaacaac
    15481 cgccaagttc tacgatgagt tctcggataa acaggttaaa accggaaaaa atcttcgaca
    15541 ttacacatta gccaatatcc ttaagaaaaa gggtattgga aaggcaaata gtgttcttga
    15601 aataggatgc ggtattggaa cccttacgga tttactcgta agtattgcac caaaggctgt
    15661 aataactgcg ttcgatatta gtccaaaaaa cattgagatt gcaaaaggcc gcataaaatc
    15721 aagtcgtgta aaattcatgg tttccgatgc atcggaaagc atttcgctaa acgaaaaata
    15781 tgacttcgtt atccttgccg atgtaattga acatattcca attgaacgtt acaattccct
    15841 attttctaac ttaaaacagt ttagccatac cggtactaaa atttttatta atattcctca
    15901 ccccaaccta attaaatatt tacgagaaac caagcccgaa agtcttcaaa ttatttatca
    15961 atctgtttac ccaacaatac tataccaaca tttatcggaa cagaattttg ttatcgatga
    16021 aaaggtagat tacagcattt tttacaaaac acctgactat acctattact ggcttagcct
    16081 tttttctgag aaaagcaatt ccaattacag taaaactgtc ccactaccct ttaggatatt
    16141 taagaaattt atactaaggg ttatttaaaa tgaatatcct ttacatttcc tcgggtaatc
    16201 ctacttttgt tcgcaaggac attttgttgc tttcaaaaca gaataatgta aaatcattgc
    16261 tatacacatg gaatacagtt aacactccaa taaatttcat aaggcaattc atctatttaa
    16321 ttccgagatt aaaaaagatt gatgtcatta tagtaatgtt tgcaggctac tggtcagtga
    16381 ttcctgcact gtttggaaaa atatttaata aaaaggtgtt cattattctt ggtggtaccg
    16441 actgtgtatc gtaccctgag tttaactatg gaagtttgcg taaaccatta cttaaaagtg
    16501 caatttacta ttctataaaa aatgccacat gcttactacc tgtccacgaa tcgctcatcg
    16561 attttaccaa ctcatactac gataaaagta aacaggggat aaaaaatcac tttaaatcca
    16621 ttaaaactga tatcaaaacc gtttataatg gatataaccc tagagtttcg catgacacaa
    16681 ttgaaaaatc aatacgcaaa cccaatagct tcattactgt agccttggtc aataataaaa
    16741 ttcgattgat tctgaagggt attgatttga ttcttgatag tgcccgcgaa tttccaaatg
    16801 caagttttac tattattggt attgccgaaa cgataaaatc tcaacttgag atacctagca
    16861 atgttataat tcatccccct atgtcgagtg aattacttgc agaaaaatat ttaaaatcgg
    16921 agtttgtaat tcaaccctca atatcggaag gttttcctaa tgcactgtgt gaagctatgt
    16981 cgtttggctg tattccaata gtatcacccg taggcgcaat gcctacaatt acagaaaata
    17041 ttggatttac tgtgaaaaat agaagcactg aggaactttc atcagtaatt aaaaatgcct
    17101 taaacctaac aaatgatgaa aaagtcaatt tacgattcct ttctcattcc aaaatagtaa
    17161 ataacttcag tgaaaaagag agaattagta aattagagca aataattgag gagtataaat
    17221 ataaatagaa tgatatcaaa atcgttcata aaatcttcag ttacctacac cattattggt
    17281 gcgcttcctt ttgcttcgag cattctcctg cttccatttt acggtaacaa
//
LOCUS       DAOOWJ010000057        17307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3308_length_17307_cov_8.749188, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000057 DAOOWJ010000000
VERSION     DAOOWJ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3308_length_17307_cov_8.749188"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(544..1224)
                     /locus_tag="PLW22_06970"
     CDS             complement(544..1224)
                     /locus_tag="PLW22_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU74483.1"
                     /translation="MKPDCKLCKMKSPAAELLRPNEIDTLEQNCAQVKFAKGETIFRE
                     GTFATNIVYLRSGLVKIHMKGPIGEKILRLNKAPAYLGLPTTFGDKVNHYSATTLVDS
                     EVCFISLDAFKKFIFSNGDFAYEIIMDLCRSELADFQRFTNLSQKQLPGRIAELLLCF
                     SNHLFKSNTFELPLNITELANFVSASRESVSRQFSELIKLGIIEITSGKKLIILRKEM
                     LEKISTNG"
     gene            1378..1923
                     /locus_tag="PLW22_06975"
     CDS             1378..1923
                     /locus_tag="PLW22_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOU74484.1"
                     /translation="MEEEMLKQLEDLKKRVSKLEKNRKDQLSIAIVSGDLDKILATMI
                     ISLAAAASDTKVKLFFSFWALSALRDQKKKAKGKSFISKMFGLMLPKGRNKLKLSKMN
                     MMGMGPAMIKFLMRKQNVMSLDEMFKQAAELGIEIVVCEMSMNLMGFKKEEIIDYPHL
                     SYAGAATFVAEASQSSIQFFI"
     gene            1938..2303
                     /locus_tag="PLW22_06980"
     CDS             1938..2303
                     /locus_tag="PLW22_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOU74485.1"
                     /translation="MSQKIVIIVTHGPDNQEKATLPFVMATAAQAMDVEVTIILQSAG
                     VLLAKKGGCIENIHAPGLMPLKELLDTFLSLNGNLLLCTPCVKERAIKEDQLIDGSKL
                     IAAGTVITETLSADAVLTY"
     gene            2323..2574
                     /locus_tag="PLW22_06985"
     CDS             2323..2574
                     /locus_tag="PLW22_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOU74486.1"
                     /translation="MNVEELKKLSVAQTVDARGTSCPGPLLAAKKAVGEIESGEILEV
                     LSSDEGTKNDIPKWCEKMEHEFLGIIEEDSYFRLFLKKA"
     gene            2634..3062
                     /locus_tag="PLW22_06990"
     CDS             2634..3062
                     /locus_tag="PLW22_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOU74487.1"
                     /translation="MKTEGYAPRILVFSTDKISDPGIDQAGLRKLHYSPSVYVISLPC
                     SSGVKPRWIMKAFEEGFDGVFIAADGHECSYSPKCAEHTNNIIVEAQRLMRERNINTK
                     RIRMAAICSVCAEPFVNHMGTFSKILAELGSVKNEIASNS"
     gene            3101..5851
                     /locus_tag="PLW22_06995"
     CDS             3101..5851
                     /locus_tag="PLW22_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU74488.1"
                     /translation="MTTNNKYDALVIGGGIAGQEAALNLADMDYKVLLVEKELSIGGK
                     MIQLSKVFPTLDCAACITTPKMSETARHGNIKVMLNSTVKEIQKHNGAFTVKVNHGAR
                     YVKPEACTGCQQCEFACPEVRPDDYNTHLAGRKVAYIPFSLANPRIAAIDRNGTSAPC
                     ISACPGGVKPYGYVSLVRNGQYEEAMNLHLEDIPLPGSLGRACYAPCQGECTRGNLEG
                     AVDIRRIKRFFAEQYYNKFPEPPTREINANTNKRVAIIGSGPAGLTAAYHLALKGHKV
                     KIFEAAPKAGGMLMLTLPEYRLPKTVVERDIKNITSLGVEIECNKRVENITELKQQGF
                     DAVFVAVGTHQSSKMHIEGENLEGVEGCLDFLRNVNIGERYNLTGKRVIVVGGGNTAI
                     DASRTALRLGAEQVTVVYRRSREEMPCFAPEIKEAEEEGVKIDILCNPIRFIGQNGKV
                     SKVELVRMKLGEPDASGRRSPIPIEGSNYLVNADLVIEAVGLQPSTQPFANELELAKG
                     GTIKVNGKTFQTNIPYIFAGGDSVSGASTIIEAAGQGKKAAFYIDKYLNGLDIDTFEF
                     DEKLPAADKNEVIAKRHPALKKPVNDTYRKPAERVKDFNDVEHTLTEQQAKESAERCL
                     DCSNCRECHQCKTACPADAIDFGQKDELITVDAKTVIVSTGFKLFKPEHKPQYGYGIY
                     PNVIDAMQMDRLIAPTRPFNNVLRPGDGKIPDKIAYILCTGSRDSSLENAMACSGECS
                     NNPICSQICCMYSIKQAQLLMGALPMADITIYYMDIRAFGKGYDEFFQEAKSMGVNFV
                     KGRIAKIFPKDQPNGDLILRYEDVTTGKLKESKHDLVVLSVGVLPNSSISRVFSNATL
                     ELDEQKFIKQIDELVNPAQTSIPGVFVAGTASGPKDIPDSILSAGTAAAEAASYINTL
                     TH"
     gene            5880..7853
                     /locus_tag="PLW22_07000"
     CDS             5880..7853
                     /locus_tag="PLW22_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOU74489.1"
                     /translation="MKQRIGVYICHCGGNISDYVDVEQLSKLIANENNVVISKDVMFA
                     CSDSNQKEMIQDIQEKQLDAIVVASCSPKLHTHTFRNVALRAGINPYNYVQVNVREQC
                     SWAHSDTPLDATVKAYGLIRAGINRVAYSEALDNIEIKAQKAVAVIGAGVAGMRAAIE
                     LARLGNNVYLIEKEEQVGGRVAQNGKLFMTGEDGKALTQRLLDNVKSYSQINLFTKAT
                     LEKVSGSVGNFNIDVNVEGNLLKLNVGAILVTTGFDYYQPSENEYGFGQTNRVITLQE
                     FNKLVENKSKELSFDDKPVNSIAYIYCVGNRQSKGPNRYCSRYCCTAAIHSSITAHEK
                     FSGIKAFHFYRDIRTYGKQELLYRQSSLNGDVYLKFEEKEPPVIEVENGYPVVKVKDY
                     LTSRKEIALKADLVVLVTGMVARSDSNSVSDKLKIPVGNDKFFNEIHPKLRPVETVIN
                     GVYIGGACQGPKNITESVQSALAGVSKINALLKKEVIELEPIVARVNKETCLWCGKCA
                     EVCEYDAIKPLEVNGKMVAEVNVATCKGCGICAPVCPNDAIEIAEYSNHGIESMIAGF
                     AAQAEINQTETQHEEKDENALVGMKEYPKEWKTILESFNGTPLSIPQISNMSGLAKEL
                     VTYHLMTMNRYGIVEPAGLNDEESYYLYKPKNH"
     gene            7856..8923
                     /locus_tag="PLW22_07005"
     CDS             7856..8923
                     /locus_tag="PLW22_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU74490.1"
                     /translation="MPTINPNFAEEIKKFGAKDFNLCFNCGNCTAICSLSTNDETFPR
                     ELIRYSTLGLEDDLKSSLKPWLCYYCGECSTDCPRQANPGELMMSLRRWLTAQYDWTG
                     LSGLLYKNLWAYILAMIAIFGAVVGISYFSEFTHSELMHIGHLFEMVAIAGVFAIILL
                     PNIIRMWYFTIIKPGIKVNLKAYFLSLKDLLIHMFTQKRFLGCEPTRENKMRWLLHLI
                     LVIGYLSLLFTTVFLNWFSTTSNFIVWLGYIESAAIFLITILFMQKRMRKVDAMHKHS
                     QPSDWFFVIWLFLMGFTAFMVRLLIDTQQIDSFFWVYVIHITVLAQWALIIVPFGKWT
                     HFLYRSFAMYFDALIMAKEKK"
     gene            complement(8967..10004)
                     /locus_tag="PLW22_07010"
     CDS             complement(8967..10004)
                     /locus_tag="PLW22_07010"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HOU74491.1"
                     /translation="MLDFSNAELTEVIVHSVGVDTDDIILSDTPLDIDDNLLNSLLRE
                     YFLGSFRNQGFYSFEHSSSLELNELYSYCSDFFEGNISFIEFSKNVANHLQHVSVHPN
                     IKGGELYIASFTDMVVDGELSDGIGIFKAESKERFLHVSRDAKGLKVTCQFGTTPRKL
                     DKACLVFNTEKDFGYKLCVIDNTNRDEAKYWLNDFLRAKLRNDDFYQTKQAINLCREF
                     VEEVVKPENNFVKVDQADILCKTRDFFKANEVFDLKEFEEVVLEEPKMAETFRDFKQK
                     YEEAIGCNIPDSFKISTDATRQSQNFFKSVVKLDKNFHIYIHGGRDRVERGFDPDTGL
                     NYYKLFFDKEV"
     gene            10224..10943
                     /locus_tag="PLW22_07015"
     CDS             10224..10943
                     /locus_tag="PLW22_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU74492.1"
                     /translation="MGKLFDRLNSDVRFVEGINGCINCGTCTAICPAAEVYDYEPRTI
                     ANLVQMRDDNVIEELLKSDTIWYCGECMSCKTRCPRNNAPGLLIQALRGLSIETGLFV
                     ESEKGRQQLSLKRMLGNSILETGYCVHFDHAELSMFPELGPVWQWVRDNREKVLAKVG
                     ANYNGSGPGAMRKISKENLDELEKIFEVTGGLDWFDKIEDVSTQKAKELGMEKGDATD
                     KKSAYFTHVYRYNNAEKHRRK"
     gene            10965..12038
                     /locus_tag="PLW22_07020"
     CDS             10965..12038
                     /locus_tag="PLW22_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOU74493.1"
                     /translation="MKQEGKKRIWSDYQKEIADDKFYFVRSCIRQTFFPGSDSTFIRI
                     LRDVLGKDIYENPDHTTCTGIGYHTEVVPFPTIQTVVARNFALMREKGYVNYTPSCVT
                     SFGIYTEILETWHHFPQEKEKTREYLRKATGREFEEPDNLAHTSDVLYKFRNEIAAKA
                     KYRLINKHTGKPLKVVDHIGCHYAKMFPSKGVGGAEYPQVLAGLVEAFGGEVIDYPER
                     RHCCGFGFRQYFVQANRGYSISASKKKFESMEPFQPDFIITNCPGCNYFMDRWQYALG
                     EMEGKTYGQNGEGIPVLTYEEVAGLVLGFDPWEMGMQMHQTDVEPLLKKMGVEYDPNQ
                     KYKGPNGEDLGVPMSPRFVNEKA"
     gene            12066..13088
                     /locus_tag="PLW22_07025"
     CDS             12066..13088
                     /locus_tag="PLW22_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU74494.1"
                     /translation="MKKVAVIGGGVTGLEAANQLAQMGYSVTVFEKKNAPGGQVSKWD
                     RLFPNRRHSIDVVNELKSHLNGNPAIYLSTAINKITKKDERFQLEAYNRIFEADAVLI
                     ATGFEPFPAERKEEYGYGIYDNVITSAELEGMFKEHGHPIMRNGNAPKRIAFIHCVGS
                     RDEKVKRPYCSKVCCVTAVKQAIEVKEAIPEAEIFCLYMDLRMFGPGYEDLYKEAQQI
                     GINFIRGRLSEASELDGGRVLIKTEDTLSSKPLKMSVDMVVLMVGMSPAKGTDELIAA
                     LGLQTNKDGFVEITDYHLNPVKTKVSGVFTAGACTGPNNITDSVNAARSAAIEIHNYL
                     NSKQYD"
     gene            13081..13416
                     /locus_tag="PLW22_07030"
     CDS             13081..13416
                     /locus_tag="PLW22_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU74495.1"
                     /translation="MIDFGFAPMPISIHNLDTANLSLAQWINQHDETFNHCIACGSCT
                     ATCTAGQFTTFSFRELCHAIRRGEVKNAIDESEKCMLCGKCTLVCPRNVNTRNIIMLI
                     RKANLTFRP"
     gene            13413..15251
                     /locus_tag="PLW22_07035"
     CDS             13413..15251
                     /locus_tag="PLW22_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOU74496.1"
                     /translation="MRYHPFTLPFSIGLGFIVLYIVVMWTIWIIRLSRREQKIVWKRL
                     FSLRTVSAIWESIREGLLHRNIFKRNILLGYMHMSLAFGWFMLIVLGNLEARLYPHGV
                     INPPFYPIFFEFFRHDLTPLHEYEEIFTFLMDFFLLMVISGVSLAFFKRFRSRTLGMK
                     RTTRHILIDRIAITALWFIFPLRLFAESVTAGLYNTGHFFTNFLGQWVIYNLPLDYLY
                     YPAWWAYSIALGVFFITMPFSRYMHIPAEILLIFLRKYGVKEKDTHSTFTKIELSACS
                     RCGICIDACQLSTSLNISDTQPAYFIRDLRYSQPSERLRETCMMCMRCLEACPVGIEI
                     TRIRENERREYPLFTSTSYSYINGYKYPKAKVAFFAGCMGHLTPSVIKSTLALLDAAG
                     VDYTFIDKNGSICCGRPLQMNGQADAAKQLIAKNNHLIVESGAEVLVTSCPICAREFA
                     SSYHLTIPVLHHSQYLLQLTKQNRLRFNQSGLKVTYHDPCELSRGLNITREPRELLQN
                     AAILSNGSKEIESKSLCCGGSLGITNISMTDRYKIANNTLDEIVTESSSYLVTACPMC
                     RKTFTNAQEKIKVLDIAEILEKNLNFAPEKASDKVQTQAKHQAVTT"
     gene            15263..16444
                     /locus_tag="PLW22_07040"
     CDS             15263..16444
                     /locus_tag="PLW22_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU74497.1"
                     /translation="MHNEKLGFNSLLIHGGMEPDPMGSATVPIYQTSTFAFENVDEGA
                     RCFAGESDGFIYTRIGNPTISALERQVAILENGFGGIATASGMGAVSSIYMAFLKAGD
                     HIVSTDAVYGPSRGIMETYFKRFGVESTYIDTSKLENIEAAIKPNTRMLYLESPTNPT
                     MVITDIKAASGLAHKHGLVVVVDNTFCSPYLQRPLDLGADVVFHSMTKFLNGHADIVA
                     GIIVTKTQELYKTVRPAMVNMGCNMDPHQAFMVLRGIKTLSLRIERAQQNAQKVAEYL
                     EKHPKIEWVKFPGLKSHPQFELAQKQMKGPGAMISFELKGGIEAGKILMNNVRLAMLA
                     VSLGGVETLIQHPASMTHSKMSPEARLKANITDGLVRFSVGIEDVEDIISDLDQALAK
                     I"
     gene            16567..>17307
                     /locus_tag="PLW22_07045"
     CDS             16567..>17307
                     /locus_tag="PLW22_07045"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU74498.1"
                     /translation="MKKYIALVSYVLMCVSVYAQVERMPFGDKMNYTANKLTIGNSKG
                     PSTPGSALFNEDFEGTTYQLTDKWEVLRSTTLDFQQTSVAANPAWFRCIPQSFNGNGS
                     TYIKHGEASGAISFTAPDFTWLITHDTIAIPSDPEAFLKFWLWYYSNYDQGYITHFYV
                     LAYDVDGQTLDTLLYYGNDQATSVPNQYKSLINLSLSKVAGKNVRLAFVYENRNNQNK
                     GTQLAIDDILVTNTNIPDIELKAIPYKYS"
BASE COUNT         5384 a         3557 c         3756 g         4610 t
ORIGIN      
        1 ttatttttgt ccatacacat aagtttgtgt agacattttt caggacgggt catttgatgc
       61 gggaatgagt cggaatgaat cggtatgatt cggaataggc agaatatgat ggagctagag
      121 gaatttagag tccgtgaaac gtgaaccgtg agtcgtgaaa cgaaagattt gatgcgggaa
      181 tgagtcggaa tgaatcggta tgattcggaa taggcagaat atgatggagc tagaggaatt
      241 tagagtccgt gaaacgtgaa ccgtgagtcg tgaaacgaaa gatttgatgc gggaatgagt
      301 cggaatgaat cggtatgatt cggaataggc agaatatgat agagttagag gaagttagag
      361 gaagttagag gaatagcaaa aagtgaacgg ctaaaccaag ttatgatgaa ttaacaaata
      421 caaccgggct aataaaaatt gtggatacat aaaatttatt agaggaaatt tatcaattga
      481 aacaagctgt ttaaatgttt tattgtgttt ctattgatta atctgggttt aaaattggaa
      541 tggttaaccg ttagtgctta ttttttcaag catctccttt ctaagaatga ttaacttttt
      601 accggaagtt atttcaataa tacctaattt gattagctcg gaaaattgtc ggctaacgct
      661 ttcgcgcgaa gcgctaacaa agttggctaa ctccgttatg tttaagggga gttcaaaggt
      721 atttgattta aaaagatggt ttgagaaaca gaggagcaac tcggcaattc ggccaggtaa
      781 ttgcttctgc gacagattag taaatcgctg aaaatcggct aattcgctcc ggcataaatc
      841 catgattatt tcataagcaa agtcgccatt ggagaaaata aattttttaa aggcatcgag
      901 ggaaataaag caaacctctg aatcaactaa ggttgtggca gagtaatggt taaccttatc
      961 gccaaaggtt gtaggtaagc caaggtatgc aggagctttg ttcaacctta atatcttttc
     1021 gccaataggg cctttcatat gaatttttac taaccccgat cgcaagtaaa caatattggt
     1081 tgcaaaggtt ccctcgcgaa aaattgtttc gccttttgca aattttacct gggcacagtt
     1141 ctgttctagg gtatcaatct cattgggcct aagcagttca gccgccggag atttcatctt
     1201 acaaagcttg caatcgggtt tcataaaaac attttttcaa atatagtcag aaagcgtgac
     1261 ttatatcaca tggttataaa atatatctca caccattttt tatttaatcc attgcattac
     1321 aacctacttt tggattgtta cttcgataac agataattaa ccaaatacct actcaatatg
     1381 gaagaagaaa tgctcaaaca gcttgaagac ctaaaaaaaa gggttagtaa gctagagaaa
     1441 aatcgtaaag atcagctgtc gatagcgata gtatcgggcg acctggacaa gattttagcc
     1501 actatgatta tttcgcttgc ggctgccgcc agcgatacta aagttaagct gtttttctct
     1561 ttctgggcgc tttcagccct cagagatcaa aagaaaaaag ccaaagggaa gagtttcatt
     1621 tccaaaatgt ttggacttat gctgcccaaa gggcgcaata agcttaaact ctcaaaaatg
     1681 aatatgatgg gaatgggacc tgccatgatc aagttcctta tgcggaaaca gaatgttatg
     1741 tcgctcgacg agatgttcaa acaggcagct gaactcggta tagagattgt tgtttgcgaa
     1801 atgtcaatga acctaatggg tttcaaaaag gaagaaataa ttgattaccc tcacctaagc
     1861 tatgccggag cggcaacatt tgtagcagag gcaagtcaaa gtagcattca atttttcatt
     1921 taaaatacca tcctattatg agccagaaaa tagttataat agtaacccat ggccccgaca
     1981 accaagaaaa agcaacattg ccatttgtaa tggcaactgc tgcacaagcc atggatgttg
     2041 aagtaacaat aatactccaa agtgccggtg ttttacttgc caagaaaggg ggatgcattg
     2101 aaaatattca tgcaccagga cttatgcctc ttaaggaatt attagatacc ttcttgagct
     2161 taaatggcaa tttactccta tgcacaccct gcgtaaagga aagagccatt aaggaagatc
     2221 aactcattga tggatcaaag ttaatagcag cgggaacggt aattactgaa acgttatcgg
     2281 cagatgccgt attaacatat taataaccaa atataaattg ttatgaacgt ggaagaacta
     2341 aagaaactta gtgtggcaca aacagttgat gcacgtggaa cttcgtgtcc tgggccactg
     2401 cttgctgcaa aaaaggcagt aggtgaaatt gaatcgggcg aaattcttga agtgctatca
     2461 tccgatgagg gtaccaaaaa cgatatccca aaatggtgcg aaaaaatgga acatgagttt
     2521 ttaggtatca ttgaagagga tagctacttc cgcctatttt taaaaaaggc ttaaaacaaa
     2581 ttggcgcaaa aggtatgtaa tccttttgcg cctttacctt taaaaaaaat aatatgaaaa
     2641 ccgaaggcta cgcaccaaga atactggtat tctcaaccga taaaatttcg gacccgggaa
     2701 ttgatcaggc tgggttgaga aaactacact attcaccaag cgtctatgtt attagtcttc
     2761 cctgctcttc tggggttaaa ccacgctgga taatgaaagc ctttgaggaa gggttcgatg
     2821 gtgtttttat agctgccgac gggcatgaat gctcttacag cccgaaatgt gccgaacaca
     2881 ccaacaatat tatagttgag gcccagcggc ttatgaggga acgaaatatc aacaccaaac
     2941 gaatccgtat ggcagccatt tgctcagttt gtgctgaacc atttgtaaac catatgggaa
     3001 cattctcaaa aattttagcg gaactaggta gtgttaaaaa tgaaattgca agtaattctt
     3061 agcatttgct aagccaaata ataaatcaaa taaggcaaaa atgacaacca ataataaata
     3121 cgatgcattg gtaattgggg gcggaattgc cggccaggag gccgctctta atcttgccga
     3181 catggattac aaggttctgc tggttgaaaa agaactttcc attggcggta aaatgataca
     3241 gctgagcaag gtattcccca ccctcgactg cgcagcctgc atcaccacgc caaaaatgtc
     3301 cgaaacggct cggcatggta atataaaggt gatgttaaac agcaccgtaa aagaaatcca
     3361 aaagcacaac ggtgctttta cggtgaaagt aaatcatggt gcccgttatg ttaaaccgga
     3421 agcctgcaca ggttgtcagc aatgcgaatt tgcatgtcct gaggttagac ccgatgatta
     3481 caatacccat ttggcaggcc gaaaggttgc ttacattcca tttagcttag caaatccccg
     3541 tattgcagca attgaccgga atggaacatc ggcaccttgc ataagtgcat gcccgggcgg
     3601 tgtaaaacca tatgggtatg tgtcgttggt gcgaaatggt cagtatgaag aagccatgaa
     3661 tcttcacctt gaagatattc ctctaccagg gagccttggc cgtgcctgct atgctccctg
     3721 ccagggtgaa tgcaccagag gcaaccttga aggtgctgtt gatatccgac gtatcaagcg
     3781 tttctttgcc gagcaatact acaacaaatt ccccgaaccc ccgactcgtg aaattaatgc
     3841 aaacacaaac aaaagggtag ccattattgg ttcgggacca gcaggattaa ctgcagcata
     3901 ccatcttgcc ctgaaaggcc ataaagttaa aatttttgag gcagcaccta aggctggagg
     3961 tatgcttatg ctcactcttc ctgagtatcg attacccaaa acagtagttg aaagggatat
     4021 taagaatatt acatcgcttg gcgttgaaat tgagtgcaat aaaagggttg agaatattac
     4081 cgaattgaaa cagcaaggtt tcgatgcagt atttgttgca gttggaactc accaaagtag
     4141 caaaatgcat attgagggtg aaaaccttga aggagttgaa gggtgtttag actttcttag
     4201 aaatgtaaat attggcgaaa ggtataacct taccggaaaa agggtaatag tagttggggg
     4261 aggaaacact gccattgatg catcgcgaac tgccctgagg cttggagcag aacaggtaac
     4321 ggttgtttac cgccgtagcc gtgaggaaat gccctgcttt gcccccgaaa taaaagaggc
     4381 agaagaagaa ggcgtcaaaa ttgatattct ttgcaaccca attcggttta taggacaaaa
     4441 tgggaaggta agcaaggttg aactggttag gatgaaactt ggcgaacccg acgcaagcgg
     4501 ccgtaggagt cccattccta ttgaaggttc caactatttg gttaatgccg atttggttat
     4561 tgaggcagtt gggttacaac catcaaccca accatttgca aacgaattgg agctggccaa
     4621 aggaggaacc ataaaagtaa acggcaaaac ctttcaaact aacatcccct atatttttgc
     4681 cggaggcgac agcgtaagcg gtgcttcaac cataattgaa gcagcaggac agggtaaaaa
     4741 agcagcattc tatatcgaca agtacttaaa tggacttgat atcgatacct ttgaatttga
     4801 tgagaaactt cctgccgctg acaagaacga ggttattgcc aagcgacacc ccgcactaaa
     4861 aaaacctgta aacgatactt ataggaaacc ggctgaaagg gttaaggatt ttaatgatgt
     4921 tgagcatacc cttacagagc agcaagccaa ggagagcgca gagcgctgtt tggattgtag
     4981 taattgtcgc gaatgccacc aatgtaaaac ggcatgccct gccgatgcaa ttgattttgg
     5041 ccaaaaggat gaactgatta cagttgatgc caaaacggta attgtttcaa ccgggtttaa
     5101 gctctttaaa cccgagcaca aaccgcaata cgggtatggc atttatccta acgtgattga
     5161 tgctatgcaa atggataggc tcatagcgcc tacccgcccc tttaacaatg ttttgcgccc
     5221 gggcgacggt aaaatacccg acaagatagc ctatattctg tgcaccggtt caagggattc
     5281 cagccttgag aatgctatgg cttgtagtgg cgaatgctcc aataacccaa tatgctccca
     5341 aatatgctgc atgtattcca taaaacaagc ccaactcctt atgggtgctt tgccaatggc
     5401 cgatatcacc atatactaca tggatatccg agcatttgga aaaggttacg atgaattctt
     5461 ccaggaagcc aaatcgatgg gagtaaactt tgtcaagggt cgaattgcaa aaatattccc
     5521 aaaagaccaa cccaatggcg atttaatact ccgctatgaa gatgtaacaa ccggaaagtt
     5581 aaaggaaagc aaacacgacc ttgttgtcct ttccgttggt gttctgccca atagttccat
     5641 ttccagggta ttcagcaatg caacccttga gctcgacgag caaaaattca tcaagcagat
     5701 agatgaattg gtaaacccag cccaaacaag cattccgggt gtatttgttg ccggaaccgc
     5761 ttcagggcct aaggatatcc ccgattctat tctatcggca ggaactgctg ctgctgaagc
     5821 cgcaagctat ataaacacgt taacacatta aaccaaccac atcgaaacat cagcaaacca
     5881 tgaagcaacg aataggagtt tacatatgcc actgcggcgg aaatatatcc gattatgtgg
     5941 acgttgaaca gctgagcaaa cttatagcaa acgagaataa cgttgttatc tccaaggatg
     6001 ttatgtttgc ctgttccgac tccaaccaaa aggagatgat acaggacata caggaaaaac
     6061 aactcgatgc aattgtggtt gcatcatgtt caccaaaact tcatacccat actttccgga
     6121 atgtggcgct aagagcagga ataaacccct acaactatgt tcaggtaaat gttcgcgagc
     6181 agtgctcatg ggctcactcc gataccccac ttgacgcaac cgttaaggct tacgggctaa
     6241 tcagagctgg tatcaatagg gttgcctact ctgaagcact tgataatatt gaaataaaag
     6301 cgcaaaaagc tgttgctgta ataggtgcag gagtagctgg catgcgcgcc gctattgagc
     6361 ttgcacggtt agggaacaac gtttatctta ttgaaaagga agaacaagtg ggtggaaggg
     6421 ttgcccaaaa tggtaaactt tttatgactg gcgaagatgg caaagctctt actcaaagac
     6481 tattagacaa tgtaaagagc tatagccaga taaacctctt cacaaaggca acactcgaaa
     6541 aagtttcggg aagcgtaggt aattttaaca tagatgttaa tgttgaaggg aatcttttaa
     6601 aacttaacgt tggagcaata ctcgtaacaa ccggtttcga ttactatcaa ccctccgaaa
     6661 atgaatacgg ttttggacaa accaataggg ttatcaccct tcaggaattt aacaaacttg
     6721 ttgaaaataa atctaaagag ttaagctttg atgataaacc agtaaatagc attgcctata
     6781 tatactgtgt tgggaacaga caatcaaaag gtccaaacag gtattgttcc cgttactgct
     6841 gcacagccgc cattcactca tcaattacag cccacgaaaa gttttccgga ataaaagcct
     6901 tccattttta tcgcgatatc agaacgtatg gtaaacagga attactttac cggcaatcat
     6961 cgttaaacgg ggatgtatac cttaaattcg aggaaaaaga accgcctgta attgaagttg
     7021 aaaatggtta cccagtagta aaagtaaagg attaccttac atcaaggaaa gaaattgcgt
     7081 taaaagctga tttggtagtc ctagtaacag gcatggttgc acgttccgat agcaatagtg
     7141 tttcggacaa gctaaaaatt ccggtaggaa acgataagtt tttcaatgag attcatccta
     7201 agctccgtcc ggtagaaacc gtaataaatg gtgtgtacat cggtggcgcc tgtcaggggc
     7261 caaaaaatat taccgagtct gtgcaatcag cacttgcagg tgtttcaaaa attaacgctt
     7321 tgctcaaaaa agaggtaatt gaactggagc caattgtggc aagagtaaat aaagaaacct
     7381 gcttgtggtg cggtaaatgt gccgaggttt gcgagtatga tgccataaaa ccccttgagg
     7441 taaatggtaa aatggttgct gaagtaaatg tagctacctg caagggttgt ggcatttgtg
     7501 cacccgtttg cccaaacgat gccattgaga tagcagagta ttcaaatcac ggtattgaat
     7561 cgatgattgc aggttttgca gctcaagctg aaatcaatca gactgaaacc caacacgagg
     7621 aaaaagatga aaacgccctt gtgggcatga aggaataccc aaaggaatgg aaaaccatac
     7681 ttgaaagttt taatggcacc ccactatcaa ttccacaaat atccaatatg tcgggtcttg
     7741 ccaaggaatt ggtaacctat cacctaatga ccatgaacag gtatggcata gttgaacctg
     7801 ctgggttaaa cgatgaagaa tcgtactatt tgtataaacc taaaaatcac tagttatgcc
     7861 cactataaac ccaaactttg ctgaggaaat taaaaaattc ggagcaaaag attttaatct
     7921 ttgttttaac tgtggaaact gcacagccat ttgctcactt tcaacaaacg atgaaacttt
     7981 tcctcgtgaa ctaattcgtt acagcacgct tggacttgag gatgacctaa aatcatcgct
     8041 caaaccttgg ctatgttact attgtggcga gtgtagcacc gattgcccca ggcaagccaa
     8101 tcctggagaa ctgatgatgt cgcttaggcg ttggctaact gcccagtacg attggacagg
     8161 gttatcaggt ttgctttata aaaacctctg ggcctacatt ctagcaatga tagccatttt
     8221 tggcgcagtt gtgggaattt catacttttc agaatttacc cattccgagc tgatgcacat
     8281 tggccatctt tttgagatgg ttgccatagc cggagttttc gcaattatac tcctgccaaa
     8341 cataatcagg atgtggtatt ttaccattat taaacctgga ataaaagtaa atctaaaagc
     8401 ttattttctt tccttaaagg acctattaat tcacatgttc acccaaaaac gattcctggg
     8461 atgtgagccc acccgcgaga ataaaatgcg ctggttgctt catcttattc ttgttattgg
     8521 ttacctctca ctacttttca caacggtctt tctaaactgg ttttccacta caagtaattt
     8581 catagtatgg ctgggttaca ttgagagtgc agcaatattt ttaattacaa ttctgttcat
     8641 gcaaaaacga atgcgtaagg ttgatgccat gcacaagcac tcccaaccat cggattggtt
     8701 ctttgtaatt tggttattcc tcatgggttt tacagcattt atggttcgtc ttttaattga
     8761 cacccagcaa atagatagtt tcttttgggt ttatgtcatt catattactg tgcttgctca
     8821 gtgggcatta ataatagtgc catttggtaa atggactcac tttttatata ggtcctttgc
     8881 aatgtatttt gatgcactta ttatggccaa ggagaaaaaa tagtgttact atgaacgaaa
     8941 aaggccggca acgtccggcc tttttattat acctctttgt caaagaaaag cttgtagtag
     9001 tttagtcccg tatcggggtc aaagccgcgc tccacgcggt cgcgcccccc atgaatgtat
     9061 atgtgaaaat tcttatcgag tttcactacg cttttaaaaa aattttgcga ttgccgggtg
     9121 gcatcggttg atatcttgaa actatcaggg atattacagc caattgcttc ctcatacttt
     9181 tgcttaaaat cgcggaaggt ctcagccatc ttgggctcct ccagaaccac ctcttcaaac
     9241 tcctttaggt caaaaacctc gtttgcctta aaaaaatcac gcgttttaca aagaatatca
     9301 gcctggtcaa cttttacaaa gttgttctca ggtttaacta cctcctccac aaactcacgg
     9361 caaagattaa ttgcctgctt ggtttggtaa aaatcgtcgt tacgaagttt ggcacgcaga
     9421 aaatcattta gccagtactt ggcttcgtcg cggttggtat tatcaattac gcaaagttta
     9481 tagccaaaat ccttttcggt gttaaaaact aagcaagcct tatcgagctt gcggggtgta
     9541 gtgccaaact gacaagttac cttcaatcct ttggcatcgc ggcttacatg taaaaaacgc
     9601 tccttgctct cggccttaaa aattccaatg ccatcagaaa gttcaccatc aacaaccata
     9661 tcggtaaaag aggcaatgta aagctcccct cccttaatgt taggatgaac tgatacatgc
     9721 tgaaggtgat tggccacatt ttttgaaaac tcaataaagc taatattccc ctcaaaaaaa
     9781 tcgctgcagt aactatacaa ttcattaagt tcaaggcttg aactgtgctc aaatgagtaa
     9841 aatccttgat tcctaaaact ccctagaaag tactccctaa gcaggctatt caataggtta
     9901 tcatcaatat ctaaaggtgt atctgataaa ataatatcat cagtatctac tccaactgag
     9961 tggactataa cctcggttag ttcagcattt gaaaagtcaa gcatggtaat tttaaattaa
    10021 tttcacgaaa ttatggattt ggaatcacct atgcaaaagc tataacaact aataatttag
    10081 acaccataaa aattactttt ataagtaatt aacattttac cacttagcta atagggtaat
    10141 tgggaaattg taaaaaattg ctaagggttc taaaaaaaat ataactttgc ttgttaacaa
    10201 actaacaatc aacccagaaa gccatgggta aactttttga caggttaaac tccgatgttc
    10261 gctttgttga gggaataaac ggatgcataa actgtggtac ctgcacagca atatgtcctg
    10321 cagctgaggt ttacgactat gaaccccgaa ccatagcaaa cctcgtccaa atgcgcgacg
    10381 ataatgttat tgaggaatta cttaaaagcg ataccatttg gtattgcggc gagtgcatga
    10441 gctgtaaaac acgatgtcca cgaaacaatg cacccggctt actgatacaa gcgctaagag
    10501 gattatccat agaaacaggg ctttttgttg aatcggaaaa gggacgtcaa cagctttcgc
    10561 taaagcggat gttgggcaac tccattctgg aaacaggcta ctgtgttcat tttgaccacg
    10621 cagaattgag catgttccca gaactgggcc ccgtttggca atgggttagg gataaccgtg
    10681 aaaaggtttt agcaaaagta ggggctaact acaatggttc ggggcctggc gccatgcgca
    10741 aaatctcaaa ggaaaacctc gacgagcttg aaaagatatt tgaagttacc ggcggcttgg
    10801 attggtttga taaaattgag gacgtatcga cacaaaaagc caaagaacta ggcatggaaa
    10861 agggtgatgc taccgataaa aaatcggcct actttaccca tgtttatagg tataacaatg
    10921 ctgaaaagca tcgccgtaag tagtttcaaa aaaggataaa ttcaatgaaa caggaaggta
    10981 aaaaacgtat atggagcgat taccaaaagg aaattgctga tgataaattc tactttgtca
    11041 ggagttgtat aaggcaaacc ttcttcccgg gtagcgattc cacctttatc agaattttac
    11101 gggatgtgct tggtaaagat atttatgaga accctgacca taccacctgt accggcattg
    11161 gttaccatac ggaggtagta ccatttccaa ccattcaaac tgttgttgcc cgtaattttg
    11221 ctctgatgcg tgaaaagggt tacgttaact ataccccatc gtgcgtaacc tcctttggta
    11281 tttacaccga gatacttgaa acatggcatc actttccaca ggaaaaggaa aaaaccagag
    11341 agtatttaag aaaagcaaca ggacgggagt ttgaagagcc tgacaattta gctcatacaa
    11401 gtgatgtgct gtataaattc cgcaacgaga ttgcagcaaa agctaagtat agattaataa
    11461 acaagcacac tggaaaaccg cttaaagttg ttgaccacat cggctgtcac tatgctaaaa
    11521 tgtttccaag caagggggtt ggtggagctg aatatccaca ggtacttgcc ggactggtcg
    11581 aagcctttgg tggtgaggta attgactacc cggaacgtag gcactgctgt ggttttggct
    11641 ttaggcaata ctttgtgcag gccaatcgag gttattccat atcggcatcg aaaaagaaat
    11701 ttgagtccat ggaacccttt cagcccgatt ttattataac taattgccct ggctgcaact
    11761 actttatgga taggtggcag tacgcccttg gcgaaatgga aggaaaaacc tacgggcaaa
    11821 acggcgaagg tattccagtg ctcacctatg aagaggttgc cggtttggtg cttggcttcg
    11881 acccctggga aatgggcatg caaatgcacc agaccgatgt agaacccttg ttaaagaaaa
    11941 tgggtgttga gtacgatccc aaccaaaagt ataaggggcc caacggcgaa gatcttggcg
    12001 taccaatgag tccccgtttt gtgaatgaaa aggcataatt aaaatatgaa aatatcagta
    12061 ctcccatgaa aaaagtagca gtaataggag gtggcgttac agggcttgaa gcagcaaacc
    12121 agcttgctca aatgggttat tctgtaacag tttttgagaa aaaaaacgct cctggaggcc
    12181 aggtatcaaa gtgggacaga cttttcccaa accgtaggca ctctattgat gtggtgaacg
    12241 aactaaaatc gcatcttaat ggaaacccgg ccatctacct atcaaccgca ataaataaaa
    12301 ttaccaaaaa agatgaacgc tttcagcttg aagcctataa ccggattttt gaagccgatg
    12361 ctgtactcat agcaacagga ttcgaaccat tccctgctga gcgcaaggag gaatacggct
    12421 acggaattta cgacaacgtg ataacctcgg cagagctgga aggtatgttc aaggagcatg
    12481 ggcaccctat tatgcgaaat ggaaatgctc ctaaaagaat tgctttcatt cactgcgtgg
    12541 gcagtcgcga cgaaaaagtg aagcgccctt actgctcaaa ggtttgttgc gttactgctg
    12601 taaagcaggc aattgaggtg aaagaggcca ttcccgaagc ggaaatattt tgcctttaca
    12661 tggatttgcg gatgtttggt ccgggttacg aggatttata caaggaagcg caacagatag
    12721 gcataaactt tatccgggga cgcttatcgg aggctagtga gctcgatggg ggtagagtcc
    12781 tcataaaaac agaggatacg ctctcctcta aacccctaaa aatgtcggtc gacatggtag
    12841 tgctaatggt agggatgtcg ccagctaagg gaactgatga actaattgca gccctgggac
    12901 ttcaaaccaa caaggacgga tttgttgaaa taactgacta tcacctaaac cctgttaaaa
    12961 ccaaggttag cggcgtattt acagcaggtg cttgcacggg gccaaacaat ataaccgatt
    13021 ccgttaatgc tgcccgttcg gctgccatcg aaattcataa ttacctaaac agcaagcagt
    13081 atgattgatt ttggttttgc cccaatgcca atcagtatcc acaaccttga tactgcaaat
    13141 ttatcgttgg cacaatggat aaaccaacac gatgaaacct ttaaccactg cattgcctgc
    13201 gggagttgta ctgcaacctg taccgccgga caatttacaa ccttcagctt tcgggaatta
    13261 tgccatgcca ttcggagagg cgaggtaaag aacgctattg atgaatcgga aaagtgcatg
    13321 ctttgtggca aatgcactct ggtttgcccg cgaaatgtaa atacccgaaa catcatcatg
    13381 ctaattcgta aagctaacct aacattcagg ccatgaggta ccatccgttc acattaccct
    13441 tttcaattgg gcttggtttt atagttctct acatcgtagt gatgtggact atttggatca
    13501 taaggcttag ccggcgtgag caaaaaatag tttggaaacg gttattctcc ctccgaacag
    13561 tctctgccat ttgggaatcg atacgcgagg ggttactcca tcggaatata ttcaagcgga
    13621 atatactttt gggttacatg cacatgagct tagcttttgg ctggttcatg cttattgtgc
    13681 tcggaaacct tgaggctcgt ttgtaccccc atggggtaat aaatccgcct ttctacccta
    13741 tcttttttga gtttttcagg cacgacctaa ccccactcca tgagtatgaa gagattttca
    13801 cttttctgat ggacttcttc ctgcttatgg tgatatcggg ggttagctta gcctttttta
    13861 aaaggttccg gagtcgcacc ctcggcatga aacgtaccac aaggcatatt ctaatcgata
    13921 ggattgcaat tacggccctt tggttcatct ttccgctaag gctatttgct gaaagcgtaa
    13981 ctgcgggact ttataatacc gggcactttt ttacaaactt tcttggtcag tgggtcatat
    14041 acaatcttcc gctcgattac ctgtactacc ctgcatggtg ggcatactct atagctttag
    14101 gtgttttctt tattactatg cccttttcac ggtacatgca cattcccgct gaaatcctac
    14161 taattttcct tagaaaatat ggcgttaagg aaaaggatac ccactcaacc ttcaccaaga
    14221 tagaattaag tgcatgttcg cgctgcggaa tttgcattga tgcgtgtcag cttagcacct
    14281 cgttgaatat ctccgatact caaccagcat atttcattcg cgatttgcgt tacagccaac
    14341 caagcgaaag gctgagggaa acttgcatga tgtgcatgcg atgccttgag gcatgcccag
    14401 ttggtatcga aataacccga atacgtgaga acgaacgcag ggagtatcca ttattcacat
    14461 cgacgagcta cagctacatc aatggatata aatacccaaa agcaaaggta gcattctttg
    14521 caggatgcat ggggcattta actccatctg taataaagtc gaccctggcc ctgctcgatg
    14581 ccgcaggtgt tgattacacc tttattgaca aaaacggaag tatttgctgt ggtaggccac
    14641 tacaaatgaa cggccaggcc gatgctgcca aacaacttat agccaaaaac aaccatttaa
    14701 ttgttgaaag tggtgccgag gtgttagtaa cctcctgtcc aatatgcgcc cgagagtttg
    14761 ccagtagcta tcaccttaca atacctgttt tgcaccatag ccagtaccta cttcagctaa
    14821 ccaaacaaaa caggttgagg ttcaaccagt cggggttaaa agttacctac cacgacccct
    14881 gtgagctgag tcgtggacta aatattaccc gagagcctcg cgaactcctg caaaatgcag
    14941 cgatcttatc aaacggaagc aaagaaattg aaagcaaatc gttatgctgt ggtggcagcc
    15001 ttggcataac caatatctcc atgaccgacc gttacaaaat tgctaacaac actcttgatg
    15061 aaattgttac tgagagtagt agttacctag taacagcctg tccgatgtgt cgcaaaactt
    15121 ttaccaacgc acaagaaaaa ataaaagtgc ttgatattgc tgaaatattg gaaaagaatt
    15181 taaactttgc acccgaaaaa gcgtcagata aagttcagac gcaagctaaa catcaagcag
    15241 taacaacata aagaaaaaag caatgcataa cgagaaactt ggatttaaca gccttctcat
    15301 tcacgggggc atggagcccg acccaatggg aagcgcaaca gtacccattt accaaacatc
    15361 aacctttgcc tttgagaatg ttgacgaagg agcaaggtgc tttgcaggcg aaagcgatgg
    15421 atttatctac acccgaatag gcaacccaac catttcggca cttgagcgcc aggtggctat
    15481 tctggaaaat ggttttggtg gaattgctac agcctcgggc atgggcgcag tgtcgtcaat
    15541 ttacatggct tttctgaaag caggtgacca cattgttagc accgatgcgg tatacgggcc
    15601 ctcacgcggt attatggaaa cctacttcaa gcggtttggg gttgaaagta cgtatatcga
    15661 cacatcgaaa ctcgaaaaca ttgaggctgc cattaagcca aacacccgaa tgttatacct
    15721 tgagagtccc actaacccca ccatggttat caccgatata aaagcagcct cggggctagc
    15781 ccataagcat gggttagtag tggtggttga taataccttt tgtagccctt acctgcagcg
    15841 tcccctcgat cttggcgccg atgtagtatt tcattcaatg accaagttcc ttaacggtca
    15901 tgccgatatt gttgctggta ttattgtaac taaaacccag gaactctata aaactgttcg
    15961 cccggccatg gtgaatatgg gatgtaatat ggatccgcac caggccttca tggtactaag
    16021 aggtatcaaa accctttcgc tccgtattga acgcgcccag caaaacgctc aaaaagttgc
    16081 cgaatacctt gagaaacacc ccaaaattga atgggttaag ttcccggggc taaaatcgca
    16141 ccctcagttt gaactggctc aaaagcagat gaagggacct ggtgccatga ttagctttga
    16201 gctaaaaggc ggcattgaag ccggtaaaat actcatgaat aatgtacggt tggccatgct
    16261 ggcggtttca cttggtggag ttgaaacatt aatacaacac ccagcctcta tgactcactc
    16321 aaagatgtcg cctgaggccc gactaaaagc caacatcacc gatggcctag tccgcttttc
    16381 cgttggaatt gaggatgttg aagacattat ttcagacctt gatcaagctt tagccaaaat
    16441 ttaatggaaa acccaaaaca atattttacc cgcaattgaa cattgcgggt tttttaatac
    16501 caaaaaaata tataccttta tttttaattt tcgtacaaaa tgtatttgaa caaaatttta
    16561 cccaatatga aaaagtacat tgcgctggta agttatgttc taatgtgtgt ttccgtttac
    16621 gcacaggttg agagaatgcc ctttggtgat aaaatgaact atactgccaa taagttaacc
    16681 attggaaatt ctaaaggccc atctactcca ggttcagccc tatttaacga agattttgag
    16741 ggtaccacct accagcttac tgataagtgg gaagtactaa ggagtacaac attagacttt
    16801 cagcaaacct ctgtggctgc taaccctgca tggttcagat gtatccctca aagcttcaac
    16861 ggcaatggat caacctatat caagcacggc gaagcctcag gggcaatatc atttactgct
    16921 cccgatttta cttggctcat tactcacgat actattgcta ttccatccga tccggaagca
    16981 tttctgaagt tttggctttg gtattacagc aactatgatc agggttacat aacccatttt
    17041 tatgtattgg catacgatgt agatggtcaa acccttgata ctctattgta ctatggtaat
    17101 gatcaagcca catcggtacc aaaccagtat aagtcgctaa tcaacctatc actttcaaaa
    17161 gtagcgggta aaaatgttcg gctagcattt gtatatgaga atcggaacaa ccagaataaa
    17221 ggaacacaac ttgcaatcga tgatattctg gttaccaaca caaacattcc agatattgaa
    17281 ttaaaagcaa ttccctacaa gtactca
//
LOCUS       DAOOWJ010000058        17275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3320_length_17275_cov_8.968873, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000058 DAOOWJ010000000
VERSION     DAOOWJ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 17275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17275
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3320_length_17275_cov_8.968873"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PLW22_07050"
     CDS             complement(<1..1211)
                     /locus_tag="PLW22_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOU74499.1"
                     /translation="MENKKELQVLFWIIFFFAFAFFMPIDNATFRTAIDATLDLTRWY
                     AREHVVLCLLPAFFIAGVISVFVSQGSVLRYFGANAKKWLAYTVAAVSGTILAVCSCT
                     ILPLFSSIHKRGAGLGPAIAFLYSGPAINILAIILTARILGIEMGIARTVGAIAFSVI
                     IGIAMSLIYRKEEQAKREGQMNIVLPPEKRPMWQTSFHFFTLVLILVFANWGKPTADD
                     TSSAWYYLYTYKWYITGFFGLLLAYSLIYILKIKWQWVAGAIGATLLSVILANMYIAD
                     PKVLPLVPMLVGIIALAFITLKDKNDDENKEWTIASWGFAKQIMPLLAVGVVTAGFLL
                     GSTHDGQTIAGVIPNEWIAYLVGGNSIFANFFASIVGAFMYFATLTEVPILQGLLASG
                     MGKGPALALLLA"
     gene            complement(1233..1934)
                     /locus_tag="PLW22_07055"
     CDS             complement(1233..1934)
                     /locus_tag="PLW22_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOU74500.1"
                     /translation="MDFILDFIYSSNIPILTAFLLGLATAISPCPLATNITAIAYISK
                     DIENQHRVFYSGLVYTLGRAITYTAIGLLFFFGASQFKIAGFLNKYGERIIGPALIII
                     GLFMLDIIKIKLGGAGKLTENFGEKAKIGNFLSVLIMGMAFALAFCPYSGVLYFGMLI
                     PITISNAKGLYLPVIYALGTGLPVIIFAYFIAFTVHGIGNLYNKVKTFELWFRRVVAV
                     VFIGVGIYYLSIFLL"
     gene            complement(1937..2368)
                     /locus_tag="PLW22_07060"
     CDS             complement(1937..2368)
                     /locus_tag="PLW22_07060"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOU74501.1"
                     /translation="MKALKYFLLAVASLTIVSCMGNSGEKNSIASTDGSGNPVTVYYF
                     HGEHRCPTCIAIENETKATFEINLKKEAEEGKVELQVINADEGRNKQICEKYGVYGSS
                     LLLVKGDKVINLTNMAFGTARRNPDKFREELSNEVKKLIKG"
     gene            complement(2371..2613)
                     /locus_tag="PLW22_07065"
     CDS             complement(2371..2613)
                     /locus_tag="PLW22_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU74502.1"
                     /translation="MEIKVLGTGCPKCKALEQATQNAINELGVQATITKVEDIVDIMN
                     YGVMRTPALVINEKVVLSGRVPDAKELKEIITKSQQ"
     gene            complement(2642..2992)
                     /locus_tag="PLW22_07070"
     CDS             complement(2642..2992)
                     /locus_tag="PLW22_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU74503.1"
                     /translation="MKSNYTEEQVKLARYAKALSHPVRVQILQILSQQSCCYSGDIVN
                     DLPIARSTLSQHLTELREAGLIQGEFNPPKIKYCIRPEAWNEAREIFDRFFNSNIWEA
                     VNTNSCSTNAGKSK"
     gene            complement(3374..4204)
                     /locus_tag="PLW22_07075"
     CDS             complement(3374..4204)
                     /locus_tag="PLW22_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74504.1"
                     /translation="MQLKLIVITFLLFPLTLASQPVIDVYFSSLAPESKGPSFSKVEE
                     VHELLIPKSGTPSYFKIVKQYNSYGKIVSEKRYNKAGGIMGESQWEYNAKGELTKKVN
                     RQFMNFKGWINEQILLTYNDTSGVLEEISLFFENQLKQRAQVIPDKDLKITEVLVFNE
                     KNVLTGKERIVYLPQNNTIRVLSYKSNEQFIGATTYPLDPKLPEPPSAIKREFNSHGD
                     IVLEALPNSIMEQGYFYEYTYDSYGNWTEKLTYQCRVTKNNKVRDKKLEYKITRKISY
                     "
     gene            complement(4347..4844)
                     /locus_tag="PLW22_07080"
     CDS             complement(4347..4844)
                     /locus_tag="PLW22_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU74505.1"
                     /translation="MSQQEFNQALIELEPNLERFAYSLTANREDARDLLQETYLKALT
                     YKDKFEDNTNIKAWTFTIMKNTFINNYRKSVKQSTTFDGSTNQFLINSKPDSFGPDAM
                     YSHSEISKKINELDDEFRIPFQMHTSGYKYKEIAEKLNLKIGTVKSRIFFSRQKLMNV
                     LKDFE"
     gene            complement(5093..5983)
                     /locus_tag="PLW22_07085"
     CDS             complement(5093..5983)
                     /locus_tag="PLW22_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU74506.1"
                     /translation="MAQYTIRQMELLSGIRASTIRMWEKRYNIFSPQRTDTNIRRYDD
                     NDLRFIINLSLLLKRGFRISKLASMSLEELSDLASKFVSDHRMGNINLEPMITATIQY
                     NETELLKHISEWSEKHGIEYTFENLVLPYLQRLGDLWQTGAITTTHEHFASNVIRNLL
                     SHLYRTCKQPEIGKSTPIIFFLPEGEFHEIGLLYFAYIAKNAGYKTIYLGQSTPIDDV
                     IRCSKDLGCKMVFTSLSSFVSADMDEAFEPMKKALPDVEIIATGHAFTEKSGPSYLTY
                     VSSAQDLMKIIARLDPTIIK"
     gene            complement(6049..6690)
                     /locus_tag="PLW22_07090"
     CDS             complement(6049..6690)
                     /locus_tag="PLW22_07090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOU74507.1"
                     /translation="MYTTNHEIDKYLESHTTPVDEVLHELTRHTYLTTYNPRMISGPV
                     QGKFIEMICQMVRPNRILEIGTFTGYSTICMAKALPAGKYIDTIEVNDELQETIEHYT
                     AKAGVSSKVNLIIGNALTVIPGLPHTYDLIYIDGEKKEYPQYLNLCTEKLNAGGFLIA
                     DNVLWNGKVADPTCTDEQTKAIRQFNKMVQENTTLENVLLPIRDGMMIVRKKF"
     gene            complement(6693..7913)
                     /locus_tag="PLW22_07095"
     CDS             complement(6693..7913)
                     /locus_tag="PLW22_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU74508.1"
                     /translation="MEFELYTLQNGIRLAHKRTDSPVAYCCIMVNTGTRDEFDHEHGL
                     AHFIEHVIFKGTKKRKAFHIMSRMEDVGGDLNAYTSKEETVIHATFLKQDFARAVELL
                     ADIMFHSTFPEKELKREKEVIVDEINSYHDSPSELIFDDFEELIFPNHPFGRNILGTK
                     KQLGKFTRKHIVDFMGRTYNTDEMVFCSIGNIPFNRIVKLAEKYFGGIHPNTRNYKRL
                     PITEYLPQRKTVKKSTYQSHCIVGTTAYDLHNPNRIAMHLVNNILGGPGMNSRLNLAL
                     RERHGLAYNVESSYTPYSDTGIFTIYFGTDKADTDKAIDLILQELDGLRNKRMGILQL
                     SKAKKQLIGQLAIGAESGENLMISTAKSILVYERPDNIQNVFKRIEEITSLEVMEIAN
                     EILSPNRLSMLIYK"
     gene            8375..8461
                     /locus_tag="PLW22_07100"
     tRNA            8375..8461
                     /locus_tag="PLW22_07100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8409..8411,aa:Ser,seq:gct)
     gene            8554..8874
                     /locus_tag="PLW22_07105"
     CDS             8554..8874
                     /locus_tag="PLW22_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74509.1"
                     /translation="MTREEFEKKFKIIQIFVAKAGFTLEAISRRYYGTLDEKKNPDGT
                     ITYYAKVHVKDKDNDSIIYSMSNDRTELQYKMDSLVVLNVYKDLHKKDGVHFIRYGMK
                     YYLN"
     gene            8874..9152
                     /locus_tag="PLW22_07110"
     CDS             8874..9152
                     /locus_tag="PLW22_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74510.1"
                     /translation="MHKFELLKRTYPISQYDRYCDGYFFILNNTTEEERNELEININE
                     LQKIIKAGEKYIYQVAKDNGEFKTMCLCFKNYEIIRKKLFKFEDYFFY"
     gene            9711..9950
                     /locus_tag="PLW22_07115"
     CDS             9711..9950
                     /locus_tag="PLW22_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74511.1"
                     /translation="MRVTKRTTLEKKSFSVKKFKQGDSLLIIYPVGCSKLSIIYKPYP
                     TKEEFNKCNDYGYYVNGLLYSKQEYEKLFGNNGTK"
     gene            complement(10215..11339)
                     /gene="amrS"
                     /locus_tag="PLW22_07120"
     CDS             complement(10215..11339)
                     /gene="amrS"
                     /locus_tag="PLW22_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU74512.1"
                     /translation="MEKISRREFLKRSLLATGGFMALRYLSLSAFGLDDKPWKWSREA
                     MFYTPTPRGMRCNLCPNECNIRPGETSQCRNRVNYNGKLYTIAYGNPCAVHIDPIEKK
                     PLYHYYPGSKSFSIATAGCNFGCLNCQNWEISQSSPKDTRNAELFPEQVVEQAKRNGC
                     KSIAYTYSEPITFYEYMYETAGIAQKKGIGNVLVSNGYINEEPLRELAKRIDAANIDL
                     KSFSNEIYLKLNAGTLEPILNTLRILLEMNVWVEITNLVIPTWTDDYAMIRKMCQWLA
                     SNGFSEVPLHFSRFFPLYKLTQLPQTPKSTLIKAREIAQSCGLKYVYIGNTALPGSGD
                     TICPSCGKVVVERKGYNVALVNINNGKCGWCGAKINGRWS"
     gene            complement(11352..13769)
                     /locus_tag="PLW22_07125"
     CDS             complement(11352..13769)
                     /locus_tag="PLW22_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74513.1"
                     /translation="MSSPYLLIPISLAITIYYLLGSLMVQAGIISFSTHRKFWNFALL
                     FVFLGCAILGILLIININYKLGWSFVKVMLQWHVQLGIAMSVVAFIHTLWHRGYFSKY
                     LLFRGRQTKTTTVNTKQQLVKSAFALGLLSVSLQVLLIRQFTKVFQGNEFLITWIIGI
                     WMLLSAAGAIAGSRTANGINPQKLTRMALGFMFLSAIIFIFLSGEIRQTFFPSGVLIP
                     PYHVIVMIVVMMTPTAFPSGMVYALLTKQIKSEYPSIYAYEALGSLAGGLLLSLVIIW
                     IFNTYLAAIAVGFLASLIIAYPLKNIARLAIPMSLLTIGISVKTLNFDIKAESMLFPG
                     QKVLSVTDSPYGSITVTGSEDQFNFFENGTMLFGTQNTIYCEEVVHYTISQRYMPSKV
                     LLVSGGYAGLIDELKKYKQVKKVDYVEPNPHLLRMSGNYCKVDFPDNTKIINSDIRGF
                     LRKSSEKYDIAIITTPEPTSLEQNRFFTIEFLELLKKHLTADGVLSVSLSGIGNYPSA
                     PRQKAYTSIAATFKSVFRNVEVISGERDYLLASDSTIRIDMAQLLSEQNIGEANLYVR
                     PDYINDEHIAQRNHFFHEQTQSTLKINTDNHPWPVLQNTLGYLSMFGDKIWLLMVVGA
                     VLLLIPFFVTNYHLRAMYAVGFAGSAIQTLYLLTLQIAAGLLYGTLGAMIALFMGGLA
                     LGSMAYGKVISIKPKHIKISLIGSFIILFALFITMMHVEIWLLIAIVSAGILTASFSV
                     GYLYISITENSNSNPNQPAKTYAADLMGSAAGIVIVTLLLIPSIGFLSSIAVLLVFIG
                     IYLFLKV"
     gene            complement(13780..15309)
                     /gene="amrB"
                     /locus_tag="PLW22_07130"
     CDS             complement(13780..15309)
                     /gene="amrB"
                     /locus_tag="PLW22_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU74514.1"
                     /translation="MYNLLNRSKKLCAFTLTVLIVTETSCNGQNMNNPNDFDRQPAVA
                     GQFYPGTKAQLEKEVKSYFDSAQKVLDAQPLALIVPHAGYVFSGQVAASGYRQIDRDK
                     KFKHIFLIGSSHTMFFTGAAVYTKGAFITPLGRVPIDPLANELVQNHKVFTDDVKPHV
                     KEHSLEVQLPFLQYWLKNDFSIIPIIIGGESQATVTLLAEALEPYLNPDNLFIISSDF
                     SHYPSYEDARHTDDDMAHAIVSNSPVEFMKAKIRNENAGIDDLATAMCGWTSGLTLLK
                     ITEGKPGITYKTIMYRNSGDSPYGGKDRVVGYWAIAAIQQQAKTVDFNLTDNDKRELL
                     KLARKTITEYLSGKTPTPPDESKLSSTLKTNAGAFVTLHNHGKLRGCIGNFSTTTPLY
                     RVVMAMAISAATEDYRFESVTADELNDIDIEISVLTPMKRISDIKEIELGKHGIYIKK
                     GGRSGTFLPQVAKETRWSLEEFLGHCARDKAGIGWDGWRDAEIFTYEAIVFDEKELGL
                     K"
     gene            complement(15542..15617)
                     /locus_tag="PLW22_07135"
     tRNA            complement(15542..15617)
                     /locus_tag="PLW22_07135"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15580..15582),aa:His,seq:gtg)
     gene            15739..17040
                     /locus_tag="PLW22_07140"
     CDS             15739..17040
                     /locus_tag="PLW22_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU74515.1"
                     /translation="MLGLVSNSTRALQVFQVMRFVAMLTIGIVMARLGFGKTAIGNYE
                     AVLFISGLLTTFWVTGLIQALLPLYTRSNDISKDDRLFNAFMVIFGLTVLACLFATVF
                     TATITGFAAINHSLFPLMLLYMLLSTPSTMAEYIYFLHGNYKAMLSYGVITYGIQIIL
                     VVLPVLFGLDVAFAVLGLIVVSAVRFIWLISLLVRNSKFRVNVDFLKDYLSDGIPLSL
                     KFLISSSGLYIDQLIIGYYYDSSTFAVYRFGAREIPLATILAASLSNSMLADFADRQR
                     LGNTLLNLKLESSMLMHILFPTSIAAILLSRWLFPLVFGAEFQESANVFMIYCLIVLS
                     RVIFPQTVTQGFRKNVAVLSISIAEMIMNIILSLLLVKYFGINGVAAATVIVYAVEKV
                     LLMAYNSYQLKISLVSYVPVKIFLAYSLLTLSVFAFSWFFL"
BASE COUNT         4733 a         3403 c         3757 g         5382 t
ORIGIN      
        1 gcaaggagta gagccagagc aggcccttta cccataccgg aagcaagtag cccttgaagt
       61 atgggaacct cggttagggt ggcaaagtac ataaatgccc ctacaatgga tgcaaaaaag
      121 ttggcaaata ttgagttgcc acctaccagg taggcaatcc actcgttggg tataacccct
      181 gctatggttt gcccatcgtg tgtcgatcca agtagaaatc cggcagtaac cacccctact
      241 gccagcagtg gcatgatttg ctttgcaaat ccccacgagg ctattgtcca ttccttattc
      301 tcgtcatcat ttttatcctt tagggtgata aaggctaaag caataatccc taccagcatg
      361 ggtacaagcg ggagtacttt gggatcggcg atgtacatat tagccagtat tactgacaac
      421 aatgtggcac caatagctcc agctacccat tgccatttta ttttaaggat gtagattaaa
      481 gagtaagcga gcaataggcc gaaaaacccg gtaatgtacc acttataggt gtataggtaa
      541 taccatgcac tcgatgtatc gtcggcagta ggttttcccc agttggcaaa aacaagtatt
      601 agtaccagcg tgaagaagtg gaacgaagtt tgccacatgg ggcgtttttc gggaggtaga
      661 actatgttca tttgtccctc gcgtttagct tgttcctcct tccggtagat tagtgacatg
      721 gctatgccaa taattacact gaatgctata gccccaacag ttcgggcaat acccatttca
      781 atgcccaaaa tgcgggctgt gagaataatg gctagtatgt tgattgcagg gccggagtac
      841 agaaaggcaa tggctggccc tagacccgct ccgcgtttgt gtatactgga gaaaaggggt
      901 aggattgtgc agctgcaaac agccagtatg gtacccgata ctgctgcaac agtgtaggca
      961 agccatttct tggcattagc cccaaagtac cttaatactg agccttggct cacaaaaacc
     1021 gaaataaccc ctgcaataaa gaaagctggt agcaggcata gcacaacgtg ttcacgggca
     1081 taccaacgcg ttaaatctag tgtggcatca atggctgttc tgaaggttgc attatcaatc
     1141 ggcatgaaaa aggcaaatgc aaagaagaat attatccaaa acagtacttg taattccttt
     1201 ttgttttcca tttcaaggtt cgtttaaaat tgctataaaa gaaaaatgct taggtagtaa
     1261 attccaacgc ctataaagac aacggctacc acacgcctaa accatagctc aaaggttttt
     1321 accttattgt ataggttccc aatgccatgt actgtaaatg caataaagta tgcaaatatg
     1381 ataactggta aacctgtgcc aagcgcatat ataaccggta agtataaccc tttagcgttt
     1441 gatattgtta taggtattag cattccaaag tagagcactc cgctataggg gcaaaaggct
     1501 aaagcaaaag ccattcccat aattagtaca ctaagaaagt tcccgatttt ggctttttct
     1561 ccaaagtttt cagtaagttt tcctgccccg cccagcttaa tctttatgat atcgagcatg
     1621 aacagaccaa taataataag tgcgggtcca attatacgtt caccgtactt atttaaaaag
     1681 ccggctattt taaactggct cgcaccaaaa aagaataaca acccaatggc tgtataggta
     1741 atggctctgc ctaaggtgta aaccaggccg ctgtagaata ccctgtgttg gttctcaata
     1801 tccttactga tatatgctat agcggtgata ttggttgcca gggggcaagg acttatggcc
     1861 gttgccaacc ctagcaggaa agcggttagt attggaatgt ttgagctgta aatgaaatcc
     1921 agaataaaat ccatggctaa cccttgatga gttttttaac ctcgttggat agttcctccc
     1981 tgaatttatc ggggttgcga cgtgctgttc caaatgccat attagtaagg ttgatcacct
     2041 tatcgccctt tacaagcagc agcgatgaac catatactcc atacttttcg caaatctgct
     2101 tgtttcgtcc ttcgtcggca ttaataacct gtagctctac ctttccttct tctgcctctt
     2161 ttttaaggtt tatttcaaag gtggctttgg tttcgttttc aatggctatg caagttggac
     2221 agcgatgttc gccatggaag tagtaaacgg taacagggtt tccgctacca tcagtgctag
     2281 caatgctgtt tttttcgcca gagtttccca tgcagcttac aatggttaaa cttgccactg
     2341 caagcaaaaa gtattttagc gctttcatac ttactgctgg cttttggtta taatttcctt
     2401 tagttccttt gcatcgggaa ctcggcccga tagaacaacc ttctcgttaa taacaagcgc
     2461 tggggtacgc ataactccgt agttcattat atccacaatg tcctcaacct ttgtgatggt
     2521 tgcctgaact ccaagttcat taattgcatt ctgggtagcc tgttcaagtg ccttgcactt
     2581 ggggcatcct gttcctaaaa ccttaatttc cattgtttga agttttaatg gtttaacatt
     2641 actattttga ttttccagca tttgtgctac atgaatttgt atttaccgcc tcccagatat
     2701 tactattaaa aaaacggtcg aatatttcac gggcttcatt ccatgcctcg gggcggatac
     2761 agtacttgat tttgggcgga ttgaactccc cttgtatcag gcctgcctcg cgcagctcag
     2821 ttaagtgctg cgatagcgta ctgcgggcta ttggtaggtc gttaacaatg tcgccgctgt
     2881 aacagcagct ttgctgcgag agtatctgga gtatctgcac ccgtactgga tggctaagcg
     2941 ctttggcgta acgcgccagt tttacttgtt cctcggtata attacttttc atctttctcc
     3001 aaaaaattct tggcaagtat acaaatattt tgtatttcgt aaaaatacga aacgattttt
     3061 tttgaacttt ttatgggtct tgtttataaa tcaatccctt ttggtgaaac tacccgttgg
     3121 ctttcatgtt aggatgccag aaaactttgg ccattttagg gaaaagtcac agtttgttaa
     3181 ctcggaaaat tcagttaagg tttttaaaat gtaatagatt aagggatgtt tgttgagaag
     3241 attgataagt gacaagtttt gcacttgcaa tatcgggcag tgtgctagaa actggtattg
     3301 ataaaattca tcaaaagata tattttggat ttgcaatagg cgagcaaatg gggtaaggcc
     3361 aaggttttag gggttagtag cttatttttc gtgtgatttt gtactctagc tttttatccc
     3421 taaccttatt attctttgtt accctgcatt ggtaggtgag tttttcagtc caattcccgt
     3481 agctgtcgta tgtatactcg taaaagtaac cttgctccat tatactgttg gggagtgctt
     3541 ccagcacaat atcgccgtgt gagttaaact cgcgctttat ggctgaaggt ggctcaggca
     3601 gtttagggtc gagtgggtag gtggttgctc ctataaactg ttcgtttgat ttgtagctaa
     3661 gtacccttat agtattgttt tgggggagat aaacaatccg ttcctttccg gttagcacgt
     3721 ttttttcgtt aaaaaccagt acttcggtaa ttttaaggtc tttgtcgggg attacttgtg
     3781 cccgttgttt aagttgattc tcaaaaaaca atgatatctc ttcaagtact ccagaggtgt
     3841 cgttataggt tagcaggatt tgctcattaa tccatccctt aaaattcatg aactgcctgt
     3901 ttaccttttt ggtgagttcg ccttttgcat tgtactccca ttggctttca cccataattc
     3961 cacctgcttt gttgtaacgt ttttcgctaa caatctttcc gtatgagttg tactgtttta
     4021 ctattttaaa gtaactcgga gttccgcttt tgggaataag caactcatgt acttcctcca
     4081 ccttgctaaa cgatgggcct ttcgattcgg gtgcaaggga actaaagtat acgtcaatta
     4141 ccggctgcga agccaatgtg agcgggaaaa gtaagaatgt gattacaata agttttagtt
     4201 gcattgcacc ttatggttta ctgcaaatat agcaattcca ttatttccac tttaagatat
     4261 ttttgggttc gtgtaagtaa actcatttat aaaatgaaag agcgctactt gtgtagcgct
     4321 cttttcggga cggataccga attatcctac tcgaagtcct ttaaaacatt catcagtttt
     4381 tgcctggaga agaagattcg gcttttaacg gtaccaattt taaggtttag cttttcggct
     4441 atctccttgt acttgtagcc cgatgtgtgc atctggaagg ggatgcggaa ctcatcgtcg
     4501 agctcattaa tctttttact aatctcgctg tgcgagtaca tggcatcggg gccaaagctg
     4561 tcgggtttgc tgttgattag gaactggttg gtgctgccat caaaggtggt gctctgcttt
     4621 accgatttgc ggtagttgtt aatgaaggtg ttcttcataa tagtaaatgt ccacgccttg
     4681 atatttgtgt tatcctcaaa cttatccttg taggttaaag ctttaaggta agtttcctgt
     4741 aacaaatcgc gagcatcctc gcggtttgct gtgagactgt atgcaaatcg ctccagattt
     4801 ggctccagct ctataagtgc ttggttgaat tcttgctgtg acatatgatt tggtttttat
     4861 ttagactagt taaaattaat ttgttgaaac aaatttagaa ttaatctaaa cagtgtgcaa
     4921 gatgatcaag atgatattta tcaattgata caaaactttt tatgggttct aaataagttt
     4981 atggcaactt gattgttttg gttggcgttg aatgaatttg tctaaatagc atgtaaagaa
     5041 aaaatcccgc cgggggcggg atttataacg ttaattagct tatttggggt ggttacttaa
     5101 ttattgttgg gtcgagtcgg gcaattatct tcatcaggtc ctgagccgat gaaacatagg
     5161 taaggtaaga tggaccgctt ttttccgtaa aagcatgtcc tgttgcaata atctccacat
     5221 cgggtaaggc cttcttcatg ggttcaaagg cctcgtccat atcggcacta acaaacgatg
     5281 aaagcgatgt aaataccatt ttgcagccta agtccttgga gcacctaata acatcgtcga
     5341 ttggtgtcga ttggccaagg taaatggtct tatagcctgc atttttggct atataggcaa
     5401 aatatagtag tccaatctca tggaattcac cttcgggtag gaagaagatg attggtgttg
     5461 atttcccaat ttcgggttgt ttgcaggtgc ggtaaaggtg gctaagtaaa ttccgaatta
     5521 cattactggc aaagtgttcg tgtgttgtag ttattgctcc ggtttgccat aggtcgccaa
     5581 gcctttggag atagggcaga accagattct caaatgtgta ctcaattcca tgcttctcgg
     5641 accattcgct gatgtgtttt agcaattcgg tttcattgta ctggatggtg gctgtaatca
     5701 tgggttcaag gttgatattg cccatgcggt ggtccgatac aaacttggag gctaggtccg
     5761 aaagctcttc aagcgacatg cttgccagct tgctaatcct aaagccgcgc ttaagcagta
     5821 acgataggtt aatgataaac cttaaatcgt tatcgtcgta gcggcggata ttggtgtcgg
     5881 ttcgttgggg tgaaaaaata ttgtagcgct tttcccacat ccttatggta gatgctctta
     5941 ttcctgagag caactccatt tgcctaatag tgtattgagc cattttgcta actgctaaaa
     6001 gtgtttatca gtttaacgct aaaatggtta aaaggtttaa caaaattatc agaatttttt
     6061 tctaacaatc atcattccat cacgaattgg cagcaataca ttttcaaggg tggtgttttc
     6121 ctgaaccatt ttgttgaact gtcggattgc ttttgtttgc tcgtcggtgc atgttgggtc
     6181 agctaccttg ccattccata aaacattatc ggctatcagg aatccaccag cgtttaattt
     6241 ttctgtacat aagttcaagt actgtggata ctcctttttc tcaccatcaa tgtatatcaa
     6301 atcgtatgtg tgggggaggc cggggattac tgtaagggcg ttgccaatga taaggttaac
     6361 cttgctggag acccctgctt tagcagtata atgttcaatg gtttcctgca gctcatcgtt
     6421 tacctcaatg gtgtcgatat atttgcctgc tggaagcgct tttgccatgc aaatggttga
     6481 gtaacctgta aatgtaccaa tctcaagtat acgatttggg cgtaccatct ggcaaatcat
     6541 ctcaatgaat ttaccctgaa cggggccgct tatcatgcgt gggttatagg tggtaaggta
     6601 tgtatggcgg gttagctcgt ggagcacctc atccaccggg gtggtgtggc tttcgaggta
     6661 tttgtcaatc tcatggtttg tggtatacat ggctacttgt atattagcat cgatagccta
     6721 ttggggctaa gtatttcatt ggcaatctcc ataacctcca acgaagttat ctcctcaatc
     6781 cttttaaata cgttctgaat gttatcgggg cgttcgtaaa ccaaaatgct tttggccgtg
     6841 gaaatcatga ggttttcccc gctttcagca ccaatggcca gttggcctat aagctgtttt
     6901 ttagctttgg agagctgcag tatacccatc cgtttatttc gtaggccatc cagttcctga
     6961 agaataaggt caattgcctt atcggtatca gccttatcgg tgccaaagta aatggtaaat
     7021 ataccggtat cgctgtatgg cgtgtacgac gattccacat tgtaggctaa gccatgccgc
     7081 tcacggagcg ccaggttgag ccttgagttc attcccgggc ctccaaggat gttgtttacc
     7141 aggtgcatgg caatacggtt tgggttatgc aggtcgtacg cagtagtgcc aacaatgcag
     7201 tgcgattggt aggttgattt cttaacggtt tttctttgtg gtaagtattc ggtaatagga
     7261 agccttttgt agttacgggt gtttgggtgt atgccaccaa aatacttttc ggcaagcttt
     7321 actattcgat taaaagggat gtttccaatt gagcagaaaa ccatttcatc ggtgttatag
     7381 gttcgtccca taaaatcgac aatgtgtttt ctggtaaact tcccaagctg cttttttgta
     7441 cctaaaatat ttcgcccaaa ggggtggttg gggaaaatta actcctcaaa atcatcaaaa
     7501 attaattcgg aaggcgaatc gtggtatgag tttatctcat caacaattac ttccttttcc
     7561 cgcttgagtt ctttctcagg gaaagtgcta tggaacatga tgtcggccag tagctctact
     7621 gcgcgggcga aatcttgctt gaggaacgtg gcatgaatta ctgtttcctc cttggatgtg
     7681 taagcattca ggtcgccacc cacatcctcc atgcggctca tgatgtgaaa tgccttgcgt
     7741 ttttttgttc ccttaaaaat tacatgctca ataaaatggg ctaggccatg ctcatgatcg
     7801 aactcgtcgc gtgtgccggt atttaccatt atgcagcagt atgccactgg cgaatcggtt
     7861 cgtttgtggg caagtcgtat cccgttttgt agtgtataga gttcaaattc cattcccgca
     7921 aaataatctg caaacctatt acatttatat ggttttacaa aaacccttaa gattgagttt
     7981 aatcgctcac ttttccacaa ttcttcaaac ttaccctaac tccttctaac tccatcgaat
     8041 tctgtttatt tcgaaaattc ctaatcatcc cgagttattc cccctctttg gcgcgcgcct
     8101 gcggacgcgt gcaagcgagg gagttgggcg gagtccaagc caaaggcagc acttcagccg
     8161 gacaaaagaa tttgtgtacg tgactgaaca agggattttt tactaaattt actgaaggct
     8221 accgggcgga gcggcgtatg ccgcacgcgt ttaaaaacga gcgggagcga gggtaataaa
     8281 gtgtaggaag taattgtcac gtactacaag tccgcgccag cagtgggggt gaaatgttaa
     8341 tgttacccca cggattgcaa atccgcgcga gcggggagag ttgggtgagt ggtttaaacc
     8401 agtagtttgc taaattacag tacgtgaaaa cgtatcgaga gttcgaatct ctcactctcc
     8461 gcatgtcaat atttaaatta attgtaaaaa aaaattgtgt tttttgtaac aaaaatttca
     8521 acaattcgta atatatataa aaaaatattg catatgacaa gagaagaatt tgagaaaaaa
     8581 tttaaaataa tacaaatatt tgttgcaaaa gcaggattta cattagaagc aattagtagg
     8641 cgttattatg gtactcttga tgaaaaaaag aatcctgatg gtactattac gtattatgca
     8701 aaagtacatg tgaaagataa agataatgat agtataatat attcaatgag taacgataga
     8761 actgaactgc aatacaaaat ggacagttta gtcgtattga atgtatacaa agacttgcat
     8821 aaaaaagacg gtgtccattt tattagatat ggtatgaaat actatctcaa ttaatgcata
     8881 aattcgaatt attaaaaaga acatacccga tatcacagta cgatagatat tgtgatgggt
     8941 atttttttat tttaaataat actacagaag aagaacggaa tgaattggaa ataaatatta
     9001 atgaattaca gaaaataata aaagcgggtg aaaaatatat atatcaagta gcaaaagata
     9061 atggtgaatt taaaaccatg tgtctttgtt ttaaaaacta tgaaattatt agaaaaaaat
     9121 tgtttaaatt tgaagattat tttttttact aaatttcttg tatctgtttg aatatttttc
     9181 tatttttata aaaattggaa tttttataag aaagaaaact ccgcttgctc ggatttgtaa
     9241 tccgtgcggt gattatttta aagataaagc agaaaaatat tatgaagtaa ataatgactc
     9301 aaaacttata attatttcag atgaacagta aaattcgtat tttatttatt tcagtatatt
     9361 ttattgtttt aatttatctc gtattagtat gcagtggcta ttactaccct ggatggaatc
     9421 cttgcttttt attcagtata ggtactttaa tggtaatttt tatcatatcg atagttggat
     9481 ttttattttc tcttatttta aaaatcaaga aaaatcgttt tgtatttgtg gggcttattc
     9541 cgttcttatt gtttattcct tgcgatatcc ttaatgaact aaatggtata gaatacatta
     9601 agcgaaatgc atgccaagtc cgtaaagtat atgttcataa tacttttagg ggttcgtcag
     9661 gtgctggttt atatcttaag actaaaaaaa gtgataaaaa atcattttat atgagggtaa
     9721 caaaacgaac aacccttgaa aagaaatctt ttagtgtcaa aaaatttaaa cagggcgata
     9781 gtcttctaat aatttacccg gtagggtgta gtaaactttc aataatatac aagccatacc
     9841 caacaaagga ggagtttaat aagtgtaatg attatggtta ctatgtaaat ggtctacttt
     9901 atagcaaaca ggagtacgag aagttgtttg ggaataatgg cacaaaatag ccccgctacc
     9961 gcacgattcc tcggccgctc gcgcggattt accttcggtg agctcccttc ggtcggttgt
    10021 aatccgtgcg gttgtagttc ggcaaagcca attcgtgcgg ttattttaaa ggtgttttca
    10081 atgaggcaca gcttgaataa gtaaaagtgt agtatgtatt tatcacgggc tataagaccg
    10141 caccagcaat gagttgcgta tgccgtacgc gtctgcagac gcgagtgagc aaggggagca
    10201 gtgcgttgaa atagctatga ccatcgccca ttaatttttg ctccacacca gccgcatttc
    10261 ccattgttaa tgttaacaag tgctacattg tatcccttgc gttccacaac caccttaccg
    10321 catgatgggc aaatggtatc gcccgatccg ggcagagctg tattgcctat gtaaacatat
    10381 ttcaacccac acgattgggc aatctctctt gccttaatta gcgttgattt tggtgtttgt
    10441 gggagttggg taagtttgta taacgggaag aaccggctaa aatgcaacgg cacttcggaa
    10501 aatccattac ttgccagcca ctggcacatc ttacgtatca tggcataatc atcggtccat
    10561 gtgggtataa ccaggttggt tatttcaacc caaacgttca tctcgagtag tatgcgtaag
    10621 gtgttaagaa taggctccag ggttcctgca ttaagcttca ggtagatttc attactaaac
    10681 gatttcaggt cgatgttggc agcgtcaatc ctttttgcga gttcgcgtag cggttcctcg
    10741 tttatgtaac cgttcgatac tagtacattg cctatgccct ttttctgagc aattccagcg
    10801 gtttcataca tgtattcgta aaaagtaatt ggctctgagt aggtgtatgc tatgcttttg
    10861 catccattgc gtttggcctg ttcaaccacc tgttcgggga atagctctgc atttcgggta
    10921 tccttgggac tgctttgcga gatctcccag ttctgacagt ttaggcaacc aaagttacaa
    10981 ccagccgttg caatggaaaa cgatttgctt cccgggtagt aatggtaaag aggtttcttc
    11041 tcaatgggat cgatatgcac ggcgcacgga ttaccgtagg caatggtgta tagctttccg
    11101 ttgtagttaa cccggttgcg gcactggctg gtttctccgg gacgaatatt gcactcattg
    11161 gggcaaaggt tgcaacgcat accgcgcgga gtgggtgtgt aaaacattgc ctcgcggctc
    11221 cacttccagg gtttatcgtc caacccaaat gccgataggc ttaagtatcg cagggccata
    11281 aaacctccgg ttgctaaaag actccgcttc aggaattcgc ggcgcgatat cttttccatg
    11341 gtgtgagttt tttaaacttt aagaaatagg taaatgccaa tgaaaaccaa taataccgca
    11401 atgcttgaca gaaatccaat tgatggtatt agcaaaagtg taacaattac aatgccagca
    11461 gccgatccca taaggtcggc agcataggtt ttggcaggtt ggttggggtt agagtttgaa
    11521 ttctcggtaa tactaatata caggtagcca acactaaacg aagcagtgag tattcctgca
    11581 cttacaatgg ctattaatag ccaaatttcc acatgcatca tagttataaa aagggcaaaa
    11641 agaataataa atgaaccgat taatgaaatt tttatgtgtt taggttttat actgattacc
    11701 ttcccgtatg ccattgagcc tagcgctagt cctcccatga aaagggcaat cattgcgcca
    11761 agcgtgccgt aaagtaagcc tgctgcaatt tgtagagtta ataggtaaag ggtttggata
    11821 gccgatccgg caaatcctac agcgtacatc gcgcgcaggt ggtagttagt aacaaagaaa
    11881 gggataagta aaaggacggc tccaacgacc ataagtagcc aaatcttatc gccaaacatc
    11941 gataggtacc ctagcgtgtt ttgcaatacc ggccaaggat gattatcggt gtttattttc
    12001 agggttgatt gggtttgctc atgaaagaaa tggttacgct gagcaatgtg ctcatcgttg
    12061 atgtaatcgg ggcgtacgta taggttggct tcgccaatat tttgctcgct tagtaattga
    12121 gccatatcaa tacgtatagt gctatccgat gcaagcaggt aatcgcgctc gccgcttatt
    12181 acctctacat ttcggaaaac gctcttaaac gtagctgcta tcgacgtgta ggccttttgc
    12241 cttggtgccg atgggtagtt cccaatacct gaaaggctaa cacttaaaac cccatctgct
    12301 gttaagtgtt tctttaaaag ttcaaggaat tcaatggtaa agaaacggtt ttgttcaagc
    12361 gatgttggct cgggcgttgt gataattgca atgtcgtact tttccgatga ttttctcaga
    12421 aaaccacgaa tgtcactatt gataatttta gtattgtccg ggaaatcaac cttacaatag
    12481 tttccactca tccttaaaag atggggatta ggttctacat aatctacttt ttttacttgc
    12541 ttatactttt tcagctcatc aatcagaccc gcataacctc ccgataccag taaaacctta
    12601 ctgggcatgt agcgttgcga tatggtgtaa tgcactactt cctcgcagta aatggtattt
    12661 tgcgtgccaa aaagcattgt tccattttcg aaaaaattga actgatcctc cgaaccggtc
    12721 acggttatac ttccataggg cgaatcggtt accgatagta ccttctgccc cggaaaaagc
    12781 atcgactccg ctttaatgtc aaaatttaat gtttttactg atatgccaat tgttaaaagc
    12841 gacattggaa tagcaagtct ggcaatattt ttaagggggt aggctatgat taaactagct
    12901 aaaaaaccaa ccgcaattgc tgcaaggtat gtattaaaaa tccagataat tacaagcgat
    12961 agcaaaagtc cccctgccaa acttcctaat gcttcgtaag cataaatgga tgggtattcc
    13021 gattttattt gtttggtgag caaggcataa accatgcccg atgggaaagc agtgggagtc
    13081 atcattacta caatcatcac tataacatgg taaggtggaa taagcactcc cgacgggaaa
    13141 aaggtttgcc tgatttcgcc cgataagaaa ataaaaatta tggctgaaag aaacataaac
    13201 cctagtgcca ttcgggttaa cttttggggg tttatcccgt tagccgttcg gcttccggca
    13261 atggcacccg cagccgacag tagcatccat atacctatta tccatgttat taggaactcg
    13321 ttcccctgga ataccttagt gaactgccta atcagcagta cctgaagcga taccgagagt
    13381 agtccaagcg caaaagctga ctttacaagt tgctgctttg tattaaccgt tgtcgttttg
    13441 gtttgcctac ccctaaacaa aaggtattta ctgaagtatc ccctgtgcca aagggtatga
    13501 atgaatgcca ccacgctcat ggcaatccca agctgaacgt gccactgtag catgacctta
    13561 acaaaactcc accccagctt atagtttata ttgattatta gtagaattcc aagaatggca
    13621 cagccaagga atacaaatag cagagcaaag ttccagaact tccgatgggt tgaaaaagat
    13681 ataatgcctg cttgcaccat taggctgcca agtagataat atatggtgat ggctaacgaa
    13741 atgggaatca ggaggtaggg tgaactcatg gacagcaatc tactttaagc ccagttcctt
    13801 ctcatcgaaa acaatggcct cgtaggtaaa tatttcggca tcgcgccagc catcccaccc
    13861 aataccagcc ttgtcgcgtg cgcagtggcc aaggaattct tcaagcgacc atcgggtttc
    13921 ctttgccacc tgtggcaaaa acgttccgga gcgacctcct tttttgatat atattccgtg
    13981 tttacccagt tctatctcct taatgtcgct tattcgtttc attggggtaa gaaccgaaat
    14041 ctcaatatca atatcgttca actcgtcggc tgttaccgat tcaaaacggt aatcctcagt
    14101 tgctgccgat atggccatgg ccattactac acggtagagt ggggttgtgg tggaaaaatt
    14161 tccaatgcat ccacgtaact taccgtggtt gtgcagggtt acaaaagcac cggcattggt
    14221 ttttagagtt gatgatagtt tggactcatc gggtggtgtg ggggttttcc cggaaaggta
    14281 ttcggtgatg gttttccgtg caagtttgag tagctcgcgt ttatcgttgt cggttaggtt
    14341 gaagtctaca gttttggctt gctgttgaat ggcagcaata gcccagtagc ctacaaccct
    14401 atccttgccg ccgtaaggcg aatcgccgga gtttctatac ataattgttt tgtaggtgat
    14461 gccaggtttc ccctcagtga ttttcaaaag cgtaagcccc gatgtccatc cacacatggc
    14521 tgttgccagg tcgtcgatac cagcattctc gtttcggatt ttggctttca tgaactctac
    14581 aggcgagtta gagacaatgg catgggccat atcgtcgtcg gtgtggcggg catcctcgta
    14641 tgaggggtag tgtgagaagt cggaactgat tatgaaaaga ttatcggggt taaggtatgg
    14701 ttctaatgct tcggccaaaa gggtaacagt ggcttgcgat tcgccaccaa taattatggg
    14761 gataatggaa aaatcgtttt ttagccagta ttgcagaaat ggcaattgaa cctcaaggct
    14821 atgctccttg acatgtggtt ttacatcatc cgtaaacacc ttatggtttt gaaccagttc
    14881 gtttgccaaa gggtcaatag gaaccctgcc tagtggcgta ataaatgcac ccttggtgta
    14941 aacggctgct cctgtaaaaa acatggtgtg gctcgatcct atcaggaaga tatgcttgaa
    15001 ctttttatcc ctgtcaattt gcctataccc gcttgcagcc acctgaccgg aaaaaacgta
    15061 accggcatga ggtacaatta gggctagggg ctgagcatcc aaaacctttt gtgcactatc
    15121 gaagtaagat ttcacctcct tttccagctg agcctttgtg ccgggataaa attgtcctgc
    15181 tacagctggt tgtctatcga aatcgttagg gttattcata ttctgtccgt tacacgatgt
    15241 ttcggttact attaaaactg ttagcgtaaa agcacaaagc tttttactac ggttaagcag
    15301 attatacatt atgctaacgg tttggtattt ccccaaattt acctttagga tagggaaaag
    15361 tcaaggggaa atgaaagaaa tgtttaagct agaatggaat agcgttttca gctgaagcgg
    15421 ttcctcttgg agtatgttca gaagttttga agaggataaa atgactggca gaaaatttga
    15481 aaataattta catgcaattt taaatctatt aaaataaaaa acccggcttt tggccgggtt
    15541 tgggtggaag atgggacttg aacccacgac ccccagatcc acaatctggt gctctaacca
    15601 gctgagctac atccaccatg tcaattgcgg ctgcaaaggt ataaacattt tttctaaatg
    15661 caaaaatgtt caaaagtaat ttgattaaac gctgtattga catcaatttt aataccttcg
    15721 cattagtaag ttataattat gttaggcctt gtatcaaatt ctacacgcgc attgcaagtt
    15781 tttcaggtaa tgcgttttgt agccatgctc accattggga tagtcatggc aaggcttggc
    15841 tttggaaaaa ctgcaatagg aaactatgag gcggtactct ttattagcgg gctacttact
    15901 acattttggg ttacagggct aattcaagca ttgcttcctc tttatacccg tagtaacgac
    15961 atttcaaaag atgaccgttt gtttaatgcc tttatggtca tttttgggct tactgttttg
    16021 gcttgcctgt ttgctacagt ttttaccgca actattactg gttttgcagc cattaatcat
    16081 agccttttcc ccttgatgct actttacatg cttctgagca ccccttcaac aatggccgaa
    16141 tacatttact ttctgcacgg aaattataag gccatgctat cgtatggcgt cattacctat
    16201 ggcattcaga taatccttgt ggttttgcct gtattgtttg gtttagatgt ggcatttgct
    16261 gtattgggtt tgatagtggt atcggcagtt cgttttatat ggttaatatc gcttttagtc
    16321 cgtaattcaa aattcagagt taatgtcgat tttttaaagg attacctaag cgatggcatc
    16381 ccgctttcct tgaaattcct tattagcagc tcaggcttat atatcgatca attaattatt
    16441 ggatactatt acgattcatc aacctttgcg gtttaccgtt ttggagcgcg cgaaatccca
    16501 ttggctacaa ttcttgcagc atcgttaagc aactccatgc tggccgattt tgccgacagg
    16561 caaaggctgg gaaatactct cttgaatctt aaactggaaa gttcaatgtt gatgcacatt
    16621 cttttcccta cctcaattgc tgcaatcctt ctatcccgat ggctattccc ccttgttttt
    16681 ggtgctgagt tccaggaaag tgctaatgtt tttatgatat actgcttaat tgtattaagc
    16741 agggttatat tcccgcaaac ggttacccag ggatttcgta aaaacgtggc tgtactatcc
    16801 atttcaattg ctgaaatgat catgaatatc attctgagcc ttttactggt taagtatttt
    16861 ggaataaatg gagtagcagc agctacggta attgtttatg ccgttgaaaa agtgttgctt
    16921 atggcataca actcctacca gttaaaaatt tctttagttt catatgtgcc agtaaaaata
    16981 tttttggcct attccctgtt aacgctttcc gtttttgcgt tttcttggtt tttcctgtaa
    17041 agctggcaga agggtaaaaa caattaagtg ggagtaacca atcaagcacc ttgttaaggt
    17101 tgcgttctta aatattgaac tgggattcaa tgttgtttag ttaaggttat ttgtaatgtt
    17161 gcgcgcaagc gaaaaataac gcaatcgtgc tgagtcccga tttataggaa tagactttgt
    17221 cgaagcatct ctttgtccct tcatcccctt tattaatcaa tgagattcct taacc
//
LOCUS       DAOOWJ010000059        17255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3327_length_17255_cov_8.266453, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000059 DAOOWJ010000000
VERSION     DAOOWJ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 17255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17255
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_17255_cov_8.266453"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1738
                     /locus_tag="PLW22_07145"
     CDS             53..1738
                     /locus_tag="PLW22_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU74516.1"
                     /translation="MKTKSLLFALLVMLGSFTACDLIDDGTDDGGSNIINSDITTNTT
                     WESGKTYVISGSIGVDGAILTIQPGVTVKFETGASLHIGYYNNATLIANGTSSKPIVF
                     TSTAANPTAGAWEGITFWDNSLNSSMQYCKVQYAGKSNEGAVNVKTCAITFSNNEVTN
                     AKYYGVMLNYEGSFTEMANNSFANCGDHPLRISAEYMHTIGVGNTFTCPADKGISVFS
                     GDATGNITWRKHSVPYFVDSDIDYDNGTLTIEPGAIFKFSAGGELHIGYYNNSTLVAN
                     GTSNNRIIFTSSAAVPAAGAWEGITFWDHSLNSSMQYCDILYAGTSSEGAVNVKTCAI
                     TFQNNTIDYAKTYGILVNSEGSFTSITNNTFSNCGDHPLRMSAKYMHTIGTGNVFNCL
                     ADKGVNIYAGDATGNITWRKLDKPYYIEGEIDFDNGTLTIEPGTILKFDANGSFHIGY
                     YNNSTLIANGNSSNYIEFTSSASSPAAGAWDCVHLWDNNTSNTSFQYCKFKYAGKGSS
                     SDRAALKASSTSFSLNNCEFSYSGGWGIYLFSSTYTGSGNTFTSCTLGDVGHN"
     gene            1847..3166
                     /gene="gldE"
                     /locus_tag="PLW22_07150"
     CDS             1847..3166
                     /gene="gldE"
                     /locus_tag="PLW22_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOU74517.1"
                     /translation="MESFSSFLNEIAQGVIVKTPSLEFYFGLLVLIILLFLSALFSGS
                     EVAYFSLTPKDFKELESRKKQSKTLKNISHPELLLATLLVANNLVNVAIVILSTFITL
                     SVLDFSHAPLVGFLFQTVIITFILLLFGEILPKILASNRSLQFAERFAPFIGLLISMF
                     KPITKLLIKSSVVISRRMATPQKISMDELGDAIELTSDGLAEERQILEGIVSFTSTMV
                     SEIMKPRLDVVALDYNTSYSRLKEVVIESGYSRIPIFNESFDDIKGILYVKDLIPHVD
                     KSDDFSWQTLIRPPYFVPESKRINDLLTEFQQKHIHLAIVVDEYGGTSGIVTLEDILE
                     EIVGEIADESDEEERLYTKVDDNNYIFEAKVMLNDFCKIVGCDDDIFDDIRGEAETIA
                     GLILENTGQIPKVNSVVKCKQFTFTIDAVDNRRIKRVKVNISPNENN"
     gene            3153..3728
                     /gene="gldD"
                     /locus_tag="PLW22_07155"
     CDS             3153..3728
                     /gene="gldD"
                     /locus_tag="PLW22_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOU74518.1"
                     /translation="MRITKVRLIFLLLFVVLVACTESPVPKPRGYFRISFPEKQYRLF
                     DTIYPFTFEYPVYGSIVKEENRQGEGEWISVSFPAYKAKIHLSYKDISGNLSQLTEDA
                     RNLAYKHTYKADAIDEQLFSNPDKRVYGILYDIKGNSASSVQFYVTDSSRHYLRGALY
                     FRCQPNKDSLAPAIDFFKADVIHLIETIRWK"
     gene            3736..4374
                     /locus_tag="PLW22_07160"
     CDS             3736..4374
                     /locus_tag="PLW22_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOU74519.1"
                     /translation="MPKANSISHDDIRIATWLITESVEELITLTGFSAPLPKSESRQR
                     EQLATAALLQSEGLPASYSYDQEGRPIIPESNVFISITHTDGLVAIAYSTNQPVGVDV
                     EHLGRDFKRVSGKYLTEHESLQSIQYSDEHFALTWCAKESIYKLPWPKSLVFSRDIEV
                     IFESETLKRCWLFAKVQNGTGWKTIKVFFTFINQYCLTWVGMNSILNGNAIR"
     gene            4355..5047
                     /locus_tag="PLW22_07165"
     CDS             4355..5047
                     /locus_tag="PLW22_07165"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOU74520.1"
                     /translation="MGTLYDKIVKGGLAILLDPDKESQVDFDAIVANGQRAGASMFLV
                     GGSLVSKPLDSFVSNLKGKTSLPVVLFPGSAMQFTPVADGILFLSLISGRNPDFLIGH
                     HVTVAPMVKQSGVEVIPTGYILIDGGAATSVQYMSQTMPIPASKPDIAVATAIAGELL
                     GLKAIYLEAGSGAKNEVPAKVIEAVRKAISVPLIVGGGIRSKQQFTDALSAGANLVVV
                     GNGLETNPRMFG"
     gene            complement(5048..6697)
                     /locus_tag="PLW22_07170"
     CDS             complement(5048..6697)
                     /locus_tag="PLW22_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOU74521.1"
                     /translation="MRLSVFISTILVLGLIETSTAQLNRSIPSEKPKLIVQLVVSQMR
                     FDYISRYWDKFGDNGFKLLVNEGTYCRNARYNYLLTQAYPGLATIATGTNPSVHGIIA
                     DKWFNRNTGNEVEATADDKVNTVGGSFFSGKYSAKNLVTSTFGDELKLNNANSKVVSI
                     ALDAGSAILLGGHSANGMYWFDTEKGLWVSSSYYTDALPAWVDTFNSKGFAKLYTDRE
                     WKALQPIQKYEEADTAAVKSVEKKISLAEKLKGMMDGIIGKPQPKTDFKVLLENPYGN
                     LLTKDLAIAAVVGENLGADEHPDLLCITFSANRFIGGKFGPHSIEMEDTYLRLDRELA
                     HFLEFINSTVGKENCLFILTSDQGVASTPDYLEKSKIPGGYFDPKKAMILLGSYLNAL
                     YGQGNWITGYHDKQIYLNRKLIEDSNLNLADFQQKVADFMLEFSGVSNSTTAHNLQNG
                     NFANGIMVKFQNSYNQRRSGDVIINLEPGWVERNGKVTSANSPYDYDSHVPLIFYGWK
                     AKRKSVLNPVYINDLAPTLSILLGITWPNGATGTPIKEMIE"
     gene            complement(6711..9275)
                     /locus_tag="PLW22_07175"
     CDS             complement(6711..9275)
                     /locus_tag="PLW22_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOU74522.1"
                     /translation="MAFNKTLLLFFITGLPCFVFPQNNEYYFRFLESNRNKVNTKITQ
                     TVSIDNIKGDTIYAYANQQEYDAFLKLGYKVEVLPHPSNLSAKAVVMATTIEEMAGWD
                     RYPTYEVYRAMMKKFETVYPTLCKLDSIGTTVQGRKLYILKISDNVESNEPEPEVFYT
                     STMHGDETTGYILLLRLIDYLLSNYNALPQVKELVDNLQIYINPNANPDGTYYGGNST
                     VVNARRSNANGIDINRNFPDPRAGQHPDNNQWQPETQAMMNFATQHQLVLSANFHGGA
                     EVVNYPWDTWTSTQEKHPDNDWFVTLSRAYVDTVHKYSPSTYMDGFENGITNGGDWYV
                     VTGGRQDYMNYYRRCREITIELSNTKLLGSELLPTLWNYNSASLINYLKSALYGFGGT
                     VKNTSNEPIDAEVIILNRDRLNSSVKTNPSNGQYFRLLEPGTYQIVAIANGYKPSLIE
                     QLTVSANEYHTVNFTLEPDNEVQATESFEQGVPERLTFTNGTWIASNTAANTGAYSLK
                     SPSTGNSQSTECILNIDVRKPGLVNFYHKVSSETGYDFFKFYIDGKLQGIWSGISNWE
                     RFTTALDVGAHLLKWVYIKDGDGTAGSDCVFIDDLSLPKCNGQINLTTTINSNAYENL
                     KIVLGDSIRTTNTSGMVAFLNYPLDTTVNLEAYSESNLLGSNQVELSWQRVNYWANLS
                     SHFQVTFEVSSKGSPLQNAIINFNGEQKQTDLNGRALFSNVPFGLQHPYSAIKDGYNP
                     ASGTVRVASDTLYSISTYPTNVDTKPFEECFSVYPNPIEGNFWVDIALTERTFIQVKL
                     TDLTGKTIAYIHTGNANPGVTRFFGDSKAMNIQPGIYIFAVDCKKFSLRKKVVFIN"
     gene            9448..9624
                     /locus_tag="PLW22_07180"
     CDS             9448..9624
                     /locus_tag="PLW22_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74523.1"
                     /translation="MVNLNVKANNIRKGLSGFLHIENLLNTEYYGQTINANWGSPKIL
                     QDLRRIDFGIEIRF"
     gene            complement(9633..9989)
                     /locus_tag="PLW22_07185"
     CDS             complement(9633..9989)
                     /locus_tag="PLW22_07185"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOU74524.1"
                     /translation="MENITYTSKDERTFSMLCHLSALSGLIIPFGHIIGPLVVWLLKK
                     EEFPEVDRQGKAALNFQLSLTIYSIIAGILVIFLVGFVLLGAIFLLGLILTIIASVKS
                     SNGERFEYPLSINLIK"
     gene            complement(10223..11896)
                     /gene="ettA"
                     /locus_tag="PLW22_07190"
     CDS             complement(10223..11896)
                     /gene="ettA"
                     /locus_tag="PLW22_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOU74525.1"
                     /translation="MADEKIIFSMVGVSKTFPPHKKVLNNIYLSFFYGAKIGIIGLNG
                     SGKSTLMKIIAGVEKSYEGEVVFSPGYTVGYLEQEPQLDENKTVREVVEEGVQEVVDL
                     LKEYEAVNARFAEPMSDDEMNKLIEKQGELTDKLEHLDAWNLDSKLERAMDALRCPEG
                     DTPVKVLSGGERRRVALCRLLLQQPDVLLLDEPTNHLDAESVQWLEMHLQQYKGTVIA
                     ITHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKSLRLAQEEKQESKRRKTLERE
                     LEWVRMSPKARHAKSKARLSAYDKLLNEDVKQKEEKLEIFIPNGPRLGDLVIEASNVS
                     KAFGDKLLFENLSFKLPPNGIVGVIGPNGAGKTTLFRLIMGLEKPDSGEFRVGETVKL
                     AYVDQQHKAIDPDKTVFEVISGGADNILLGGRQINARAYVARFNFSGADQEKKCGMLS
                     GGERNRLHLALALKEEGNVILLDEPTNDIDVNTLRALEEGLEDFAGCAVVISHDRWFL
                     DRIATHILAFEGNSQVYFFEGTYSEYEENKKKRLGDDTPHRIKYRKLME"
     gene            complement(11977..12822)
                     /locus_tag="PLW22_07195"
     CDS             complement(11977..12822)
                     /locus_tag="PLW22_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74526.1"
                     /translation="MKKLSLIIGLVTITLWGMAQRNLVRPEDVKAFMASTTYVVLENN
                     PTSQFNIEIREAVERSWKITPYKFITAKEFNDMRKDIDKSFLVLIQMRFDKDKIAPTY
                     NFLSVSMGAAVSKITDMPDICSVPLSYKDLSEDTYTYKLEGIIRFMQNYITAINENPK
                     LIKKDAFKFYNKNMKQVKSKELWVTESALDKTVRPLAEIRKVYKFNIKVVNPEDIQKA
                     IQEKNPNVLYLHKVGPEGSRLQWRVWKMILGAGDDQLYYYDMHTISKSKGDGFLSKDF
                     AKINK"
     gene            13073..14911
                     /locus_tag="PLW22_07200"
     CDS             13073..14911
                     /locus_tag="PLW22_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU74527.1"
                     /translation="MTESRNKIVNADEIKKAVNLGPIIGDGFARLLMYILRFNELNKK
                     YANVANLQGLEFLNAALDILEVKFEVNPEELKRIPKNGAFISVSNHPFGGMDGILLLK
                     IMLEQQRDDIRLLSNFLIKRIPQVEKFVFAVDPFENGGYQKNSNISGLKAAYTHLSEG
                     GALGLFPAGEVSTYYDNVDETGVTDRHWQRSMIKFIKNARVPVVPIYFQGENSRLFHL
                     LGLIHPLLRTAKLPSELLNKKNQTIRVRIGYPISVEEQDNYCDIEKFGRFLRVKTYSL
                     GSTIDVDKFFKYKLKSEPEPEPIIPPVPQEVIEDEVSKLIENYLLFRSKNYAVICAPS
                     VEMPNLMTEIGRLREITFRDVGEGTNRKIDVDEFDLYYWQLFIWDEDERRIVGAYRAG
                     KGKEIIEKYGIEGFYIQTLFKIDKAFAPILEQCIELGRSFIVPDYQRKPLPLFLLWKG
                     ILYFLIKNPEYRYLIGPVSISNRFSDYSKGVIISFMKSNYYDHDFARFIKSRNKFKVP
                     IKDEEFDIIFNNSDDLGKLDKFIQEVEPYDYRMPVLLKKYVKLNGKIIGFNVDPKFNN
                     ALDGLLILDLFQVPAETITSLSKEINDKSILERFNITDYTFEDQAE"
     gene            15006..16232
                     /locus_tag="PLW22_07205"
     CDS             15006..16232
                     /locus_tag="PLW22_07205"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOU74528.1"
                     /translation="MNRYFLIFLAFAFLGCSNRTVTDDAKLEQRAKALHSKMVTIDTH
                     TDTPLNFLRTGFDFTGQNNTAIGSKVDLAKMEQGGLDAAFFAVFIGQRECTPEMYAQV
                     NAKAKEIFDAIYTTVNKHSNRASIATKPDDVYRLKKEGKRAIYIGVENGYPIGDDLSQ
                     VKLFYDMGARYITLCHTRNNQICDSSTDPDGPRHNGLSQFGREVVAEMNRLGMIVDVS
                     HISDKAFYDVLEVSKAPVIASHSCSRAICDNPRNLTDDMLRSIARNGGVVQMCILSEY
                     VKKGEPNPARDSAYRALRRKWNNFRGLTPEQEKQATTEWYELEEKYPASLATVADVVD
                     HIDHMVKVMGIDHVGIGTDFDGGGGVDGCADASQMYRITMELLRRGYSERDIRKIWGE
                     NFLRVFREVEKRKERG"
BASE COUNT         4764 a         3667 c         3844 g         4980 t
ORIGIN      
        1 tttaaagaat ttctcattct ttaatcaaaa ctaatattaa aatcaaatca agatgaaaac
       61 aaagtctcta ttatttgcat tacttgtcat gctaggtagt tttacagcat gcgatcttat
      121 tgacgatggt accgatgatg gtggatctaa catcattaac tccgacatta caacgaatac
      181 tacttgggag tcggggaaaa cctatgtaat tagcggcagc attggagttg acggtgcaat
      241 attaaccata cagcccggcg ttacagtaaa atttgaaaca ggagcaagtt tgcatattgg
      301 gtattacaac aacgctaccc taattgctaa tggtacatcc tcaaaaccta tagttttcac
      361 ttcaactgct gctaatccta cagcaggggc atgggaaggt ataacttttt gggataacag
      421 cttaaattcg agtatgcagt actgtaaagt tcagtatgct ggaaaatcaa acgaaggggc
      481 agttaatgta aagacctgtg ctattacatt tagcaacaat gaggttacca atgctaagta
      541 ttacggcgtt atgctaaact acgagggctc tttcactgag atggctaaca attcatttgc
      601 aaattgcggt gaccatcctc tcaggatttc tgctgagtat atgcatacca ttggtgttgg
      661 gaacacattt acatgcccag ccgacaaggg tattagcgta ttcagtggcg atgcaaccgg
      721 caatattacc tggcggaagc atagcgtacc atactttgta gatagcgata ttgattatga
      781 caatggtaca ctaaccattg aacccggagc tatttttaag tttagtgctg gaggagaact
      841 tcatattggc tattacaaca attcaacact ggtagcaaat ggtacctcca acaacaggat
      901 aatttttacc agtagcgcag ccgttccagc cgcaggtgcg tgggagggta taacattttg
      961 ggaccatagc cttaactcca gcatgcagta ctgcgacatc ctttatgcag gaaccagtag
     1021 tgagggggct gttaatgtta aaacctgtgc aattacgttt cagaataata ctattgatta
     1081 cgcaaagact tacgggatac ttgtgaactc agaagggagc tttactagta ttacaaacaa
     1141 tacttttagc aattgtgggg atcatccatt acgtatgtcg gcaaagtaca tgcacactat
     1201 tggaaccggt aatgttttta actgtctggc cgataagggt gtaaacatat acgcaggcga
     1261 tgcaactgga aacataacat ggcgcaagct cgataaacct tactacattg aaggtgaaat
     1321 tgactttgat aatggtaccc ttaccattga accgggcact atactgaaat ttgatgccaa
     1381 tgggtcgttc cacataggct attacaacaa ttccactctt attgccaatg ggaattcaag
     1441 caactacatt gagtttactt caagcgcttc atcgccggct gccggcgcat gggattgtgt
     1501 acacctttgg gataacaaca cctctaatac cagtttccag tactgcaagt tcaagtatgc
     1561 aggcaaaggt agctccagcg atagggctgc cctaaaagct tcgagcactt ctttttcatt
     1621 aaataactgc gagtttagtt actctggggg ttggggtatt tatctgttta gctctaccta
     1681 tacaggatct ggcaatactt ttacatcctg tacgctgggc gatgttggcc ataactaaat
     1741 tttaacctct tatattagtt tttccaagag tagcgcaaag ttaaatactt tgcgctattt
     1801 attatttttg ctaactttac agggcaaacc aatacccact ttaattttgg aaagttttag
     1861 tagtttcctt aatgagattg cccagggagt tattgtaaaa accccatcac ttgaattcta
     1921 ttttggccta ttggtgttaa ttatactact ttttctctcg gcgcttttca gcggttccga
     1981 ggtagcctac ttttcgttaa caccaaagga ttttaaggag cttgagagca ggaaaaagca
     2041 atcaaaaacc ctaaaaaaca tctcgcaccc cgagttactc ctggcaacct tactggttgc
     2101 aaataaccta gttaatgtag ccattgtaat actttctacc tttataaccc tatcggttct
     2161 tgattttagc catgcaccct tggtcggttt tctgtttcaa acggttataa ttactttcat
     2221 actgcttcta tttggtgaga ttcttcccaa aatattggca tcaaataggt cattacaatt
     2281 tgccgagcga tttgcgccct tcattgggct gctcatttca atgtttaagc caattacaaa
     2341 attactcatt aagtcgtcgg tggttataag taggcggatg gccactcccc aaaaaatctc
     2401 aatggatgag cttggcgatg ccatagagct tacttccgat ggtctagccg aggaacgaca
     2461 aattcttgag ggaatagtat cgttcaccag caccatggtt agtgagatta tgaaaccccg
     2521 actcgacgtg gttgcacttg actacaacac ctcctatagt aggcttaaag aggtggttat
     2581 tgaatcgggc tatagccgaa tacccatttt taatgagtcg ttcgatgata taaagggaat
     2641 actttacgtt aaggatttaa tcccccacgt tgacaagagc gacgattttt cctggcaaac
     2701 actcatcaga ccaccctatt ttgtaccgga aagcaagcga attaacgact tgctcactga
     2761 gttccagcag aaacatatac accttgctat agtggtagac gagtatggcg gcacaagcgg
     2821 aattgttacc cttgaggata tacttgagga gattgttggc gaaattgccg atgaatccga
     2881 cgaagaggaa cggctttaca caaaggttga cgataacaat tacatttttg aggccaaggt
     2941 gatgctaaac gacttttgta agattgttgg gtgcgatgat gatatctttg acgatattcg
     3001 tggcgaagcc gaaacaattg caggcttaat tctggaaaac accgggcaaa tccctaaggt
     3061 gaactcagtg gtaaaatgca aacagttcac tttcactatt gatgcagtgg acaaccgcag
     3121 aattaaacgg gttaaggtta atattagtcc caatgagaat aactaaggta agattgatat
     3181 ttttgcttct ttttgtcgtg cttgtggctt gcactgaaag tccagttcct aaaccgcgtg
     3241 ggtatttccg tatatcgttc cccgaaaaac aataccgatt attcgatacc atttacccct
     3301 ttacttttga gtatccggtt tatggttcca ttgtaaagga agaaaaccga cagggtgagg
     3361 gtgaatggat aagcgtgagt tttccggcct ataaggccaa gatacacctg agctataagg
     3421 atatttcggg caatttgagc cagttaaccg aagatgcacg taacttagcc tataagcata
     3481 cctacaaggc cgacgccatt gatgagcagc ttttctcaaa tcccgataag agggtttatg
     3541 gcattctgta cgacatcaaa gggaactctg ccagttcagt gcagttttac gttactgact
     3601 caagcaggca ttacctacgt ggagcacttt acttccgctg ccagcccaat aaggactccc
     3661 ttgccccagc catcgacttt tttaaagcag atgtgattca tttgattgaa accattaggt
     3721 ggaagtagag ttcttatgcc caaggctaac tccatatcac atgacgatat acggatagcc
     3781 acatggctaa tcacagaaag cgttgaggaa ctgataaccc ttacgggttt ttcggctcct
     3841 ttaccaaaga gtgaatcgcg ccaaagggaa cagcttgcaa cagcagccct gctgcagtcc
     3901 gaggggttac ctgcaagtta cagctacgac caggaagggc ggcctattat tcctgagtca
     3961 aatgttttta tctccattac ccacaccgat ggtctcgtag ccattgcata ctctaccaat
     4021 caaccagttg gtgttgatgt agagcactta ggtcgtgatt tcaaaagggt ttcagggaaa
     4081 taccttactg aacatgaatc tttacaatca attcaatact ccgatgagca ctttgcccta
     4141 acctggtgtg caaaggaatc catctataag ctaccatggc caaaaagttt agtgtttagt
     4201 cgtgatattg aggtgatatt tgaatccgaa acgctaaaac gttgctggct ttttgctaaa
     4261 gtacaaaatg gtacagggtg gaaaacaata aaagtttttt ttacctttat caatcagtat
     4321 tgccttacat gggtgggtat gaatagtatt ttgaatggga acgctatacg ataaaatagt
     4381 aaagggtggc cttgccatac tgctcgatcc cgacaaggag tcgcaggtgg actttgatgc
     4441 cattgttgcc aatgggcaac gggctggagc gtcgatgttt cttgtaggcg ggagtttggt
     4501 ttccaaaccg ctcgatagct ttgtaagcaa cctaaaggga aaaacatcgt tgcctgtggt
     4561 gctattcccc ggaagtgcca tgcagtttac acccgttgcc gatggtatcc tttttttatc
     4621 gttaatatcg ggtcgtaatc ccgatttttt gataggccac catgtaactg tagccccaat
     4681 ggtaaagcag agtggcgttg aggtaatccc aacagggtac attctaattg atgggggagc
     4741 cgccacatcg gttcaataca tgagccaaac catgcccatt cccgcaagta agcccgacat
     4801 agcagtggct accgccattg caggcgaact tctgggacta aaagccattt accttgaggc
     4861 gggtagtggg gcaaaaaacg aagtacctgc taaggtgatt gaggctgtaa ggaaagccat
     4921 ttcggttcca ctcattgttg gtggaggcat acgcagtaag cagcagttta ccgatgctct
     4981 tagtgccgga gctaaccttg tagtggttgg taatgggctt gagacaaacc cgcgaatgtt
     5041 tggttaacta ctcaatcatc tccttaatgg gcgttccggt tgcgccgttt ggccaggtaa
     5101 tcccaagaag tatggagaga gttggtgcca aatcattgat gtaaaccgga ttaagtaccg
     5161 atttgcgttt tgccttccat ccatagaata ttaatgggac atgtgaatcg tagtcgtagg
     5221 gtgagtttgc cgaggttact ttcccgttac gctcaaccca tccgggttca aggttgatga
     5281 ttacatcgcc ggagcgacgt tggttgtagc tattctggaa cttaaccatg attccatttg
     5341 cgaaattgcc gttttgtagg ttatgcgcgg tggtgctatt ggaaacgccg ctaaattcta
     5401 gcataaaatc agcaaccttt tgctggaaat cggcaaggtt aaggttggaa tcctcaataa
     5461 gtttacggtt caggtagatt tgtttatcgt gatagccggt aatccagttt ccctgtccgt
     5521 agagtgcatt gaggtaactt cctagtagta tcatagcctt tttggggtcg aagtaaccac
     5581 caggaatctt cgatttttca aggtaatcgg gggttgaggc tacaccttgg tcggaggtta
     5641 gtataaaaag acagttctcc ttaccaacag ttgagttgat aaactcaagg aaatgggcaa
     5701 gttccctatc gaggcggagg taggtatcct ccatctcaat ggagtgtggg ccaaatttgc
     5761 caccaataaa tcggttagcg ctaaaggtaa tgcaaagcaa gtcgggatgc tcatcggccc
     5821 caagattttc acccactacc gctgctatgg cgaggtcctt ggtgagtagg ttgccgtagg
     5881 ggttttcaag taaaacctta aagtcggttt tgggttgggg ttttccaata atgccatcca
     5941 tcataccttt tagcttttca gctaaactta tcttcttctc aactgacttt acagcagcgg
     6001 tgtcggcttc ttcgtatttc tgaattggct gtaaagcctt ccattccctg tcggtataga
     6061 gtttagcaaa acctttgcta ttaaaagtat ctacccacgc cggaagtgcg tcggtgtagt
     6121 agctgctgct aacccataaa cctttttcgg tatcgaacca gtacatacca tttgcgctat
     6181 gcccacctaa aaggattgcc gaccctgcat caagggcaat gctaactacc ttggagtttg
     6241 cattattcag ctttagctca tcgccaaagg tgctggtgac caaattcttt gcagagtatt
     6301 taccgctaaa aaagctgcca ccaacggtat tcaccttatc atcggcagtt gcctcaacct
     6361 catttcctgt attgcggtta aaccatttat cggcaataat tccatggacc gatgggttag
     6421 ttcctgtggc aatggttgcc aaacctgggt aagcttgagt aagtaggtag ttataacgag
     6481 cattgcggca gtaggttccc tcgttaacaa ggagtttgaa cccgttatcg ccaaacttat
     6541 cccagtagcg cgagatgtaa tcgaatcgca tttggctgac tactagttgt actatgagct
     6601 ttggcttttc cgagggtatg gaacggttta gctgtgcggt tgaggtttca ataagtccta
     6661 ataccaagat tgttgaaata aatactgaaa ggcgcatggt taaatcgggt ttaattaata
     6721 aaaactactt ttttacgtaa agaaaacttt ttgcaatcaa ccgcaaaaat ataaatgccg
     6781 ggttgaatat tcattgcctt gctatcgcca aaaaatcgtg ttactcccgg attagcattg
     6841 ccggtatgga tataggcaat ggttttaccc gttaaatcgg taagttttac ctgaatgaat
     6901 gttctttcag taagtgctat atcaacccaa aaatttcctt ctatagggtt tgggtaaact
     6961 gaaaaacact cttcaaatgg ttttgtatca acatttgtgg ggtaagtgga gattgaatat
     7021 agggtatcgc tggctaccct taccgtgcca gaggcggggt tatacccatc tttaattgct
     7081 gagtatgggt gttgtaaacc aaatggaaca ttgctgaata gtgctctacc gttaaggtcg
     7141 gtttgtttct gttctccatt aaagttgatg attgcgtttt gcaatgggct tcccttagag
     7201 ctaacctcaa aagtgacctg gaagtgagag ctgaggttag cccagtagtt aaccctttgc
     7261 cacgataatt caacctgatt gctacctaaa aggttgcttt cggagtaggc ttcaaggttt
     7321 acagttgtat cgagcggata gttcaagaat gcaaccatcc cgctagtgtt agttgttctg
     7381 atgctatcgc caaggacgat ttttagattc tcgtaagcat ttgagttgat agtggtagtt
     7441 aggtttattt ggccattgca cttgggtagc gataaatcat cgataaaaac gcagtcggaa
     7501 cctgccgttc catccccatc ctttatgtat acccacttaa gcaaatgcgc tccaacatca
     7561 agcgctgtag taaacctttc ccagttgcta ataccactcc aaatgccttg aagcttacca
     7621 tcaatataaa acttaaaaaa gtcgtaacct gtttcggaag aaaccttatg atagaagtta
     7681 actagtccgg gttttctgac atcgatgttt aggatacatt cggttgattg cgagttacca
     7741 gtagagggcg attttaagct ataggctcca gtgtttgctg cagtattact tgcaatccag
     7801 gttccattgg taaatgttaa acgttctggt acaccctgtt caaaactttc ggttgcttga
     7861 acttcgttgt cgggctctag tgtgaaattt accgtatggt attcatttgc cgataccgtt
     7921 agctgttcaa ttaagcttgg tttgtagccg tttgcaatag ctacaatttg gtaggtacct
     7981 ggttctaaca atctaaagta ctgtccgttt gaagggttag ttttaacgga tgagtttagc
     8041 ctgtcgcgat tcaatattat tacttcagca tcgatgggct cgttcgaggt attcttaaca
     8101 gttcctccaa acccataaag ggcacttttt agatagttga taagcgaagc gctgttgtag
     8161 ttccaaagtg ttggtagcag ttcagagcca agtagttttg tattggataa ttcaatggta
     8221 atctcgcggc agcggcggta gtaattcatg taatcctgcc ttccaccagt aaccacatac
     8281 caatcgcccc cattggttat tccattttca aatccatcca tgtatgttga tgggctgtat
     8341 ttgtgaacag tatcaacgta tgcccttgaa agggttacaa accaatcgtt atcggggtgt
     8401 ttttcctgtg tcgacgtcca ggtatcccag gggtagttta ccacttcagc accgccatgg
     8461 aagtttgccg aaagcactaa ttgatgctgc gtcgcaaagt tcatcatggc ctgtgtttcc
     8521 ggttgccact ggttgttgtc ggggtgctgg ccggctcgcg ggtcagggaa gttgcggttg
     8581 atatcaatgc cgttagcatt ggaacgcctt gcattcacaa cggttgaatt accgccgtag
     8641 tacgttccat cggggttggc attagggttg atatagattt gcaggttatc aaccagttcc
     8701 ttaacctggg gcagggcgtt gtagttcgat agtaggtagt caataagcct aagcagtagg
     8761 atatatccag tggtttcatc gccatgcatg gtgctggtat aaaagacctc gggttcaggc
     8821 tcattgcttt ccacattatc ggaaattttt aaaatatata gtttgcgtcc ctgaacggta
     8881 gttccaatag aatcgagctt acagagggta gggtagactg tttcaaattt tttcatcatg
     8941 gccctgtaaa cctcataggt gggataccta tcccatcctg ccatctcctc aatggttgtt
     9001 gccattacaa cggccttggc acttaggttc gatggatggg gaagcacttc aaccttatac
     9061 cctaacttta gaaaagcgtc atattcctgc tggttggcat aggcataaat agtatctccc
     9121 ttgatattat caattgatac ggtttgggtg attttggtgt ttaccttgtt gcggttagac
     9181 tcaagaaacc taaagtaata ctcgttgttt tgtgggaaaa caaaacaggg caaaccagta
     9241 ataaaaaaaa gaaggagggt tttgttaaat gccatattag gtgatttttg atgttgtaaa
     9301 cttaagtaaa atttgataga tttgagtgaa atccttaagt caaaatgaag tgtaatatca
     9361 acaaaatcaa cctgaaactt ttgagcaatt atattctata acaactgcca acgttatggg
     9421 ctatttgtta atttcgatcc agttcttttg gtaaatctta atgtaaaagc gaataatatc
     9481 cggaaaggac tatcgggttt cctgcacatc gagaacttac tcaacaccga atactatggg
     9541 caaaccataa atgcaaactg gggaagccct aaaatacttc aggacctccg cagaattgat
     9601 tttgggattg agattaggtt ctagccgact ttttacttta tgagatttat ggaaagtggg
     9661 tactcaaaac gctcgccatt cgacgatttt accgatgcta taatggttaa aataagccct
     9721 aaaaggaaga tagcacccag tagcacgaaa ccaaccaaaa agattacaag aatacctgct
     9781 ataattgagt aaatggtaag cgatagctga aagtttaacg ctgccttacc ctgccggtcg
     9841 acctcaggga attcttcttt ttttagtaac caaacaacaa gtggcccaat aatatgccca
     9901 aaaggaatta ttaatcccga gagtgctgaa agatgacaga gcatgctgaa ggtgcgctca
     9961 tccttacttg tgtaagtaat gttttccata atgaaagtat ttataccaaa attacgaaaa
    10021 gttagaacga tttgctgcat ttgtaataaa aattgcattt aaatgttgga ttaatagaat
    10081 tttgacatca tgtttaatgt gaaaaaagtt tattaacctt aaaggcttaa ttattctaag
    10141 tagccctgtt aaagttgaag ttaactaaat gcagaatgcc taattcacaa aaaaaatacc
    10201 ggccttaaag ccggtattta gtttattcca ttagcttgcg gtacttaatc ctatggggtg
    10261 tatcgtcgcc aagccgtttc tttttgtttt cctcatactc tgagtaggtt ccttcaaaga
    10321 agtaaacctg ggagttaccc tcaaaggcaa gtatgtgagt agcaatacgg tcgaggaacc
    10381 aacggtcgtg cgagattacc acggcgcagc cggcaaagtc ctccaatcct tcctcaaggg
    10441 ctcgcagagt gttcacatcg atatcgttgg taggctcatc gagcagaatt acgtttccct
    10501 cctctttaag ggcaagagca aggtgaagcc tgttccgctc cccacccgat agcatgccac
    10561 atttcttttc ctgatcggca ccggaaaagt tgaaacgtgc cacgtatgcg cgggcgttta
    10621 tttgacggcc gcccagcaag atattatcgg cgccgcccga tatcacctca aataccgttt
    10681 tatcggggtc aattgctttg tgttgctggt caacataggc tagctttacg gtttcgccta
    10741 cacggaattc gccgctatcg ggcttttcaa gccccataat aaggcggaag agtgtggttt
    10801 tacctgcacc gttggggcca ataacaccta ctattccgtt tggtggaagc ttaaacgaaa
    10861 ggttttcgaa aagcagctta tcgccgaaag ccttggaaac gttgctagct tcaattacca
    10921 aatcgccaag tcgaggcccg ttgggaatga aaatttcaag cttttcttcc ttctgtttta
    10981 cgtcctcgtt caggagctta tcgtatgccg agaggcgggc ttttgacttg gcatgacggg
    11041 ctttaggcga catacgcacc cattccagct cgcgctcaag ggttttacgg cgtttgctct
    11101 cctgcttctc ctcctgtgcc aggcgtaacg atttttgctc cagccatgag gagtagttcc
    11161 ctttccaggg aattccttcg ccacggtcga gttccaggat ccaacctgca acgttatcga
    11221 ggaagtaacg gtcgtgggta atggctataa cagttccttt gtactgctgt aggtgcatct
    11281 cgagccactg aaccgactct gcgtccagat ggttggttgg ctcatccagc aacagtacat
    11341 cgggctgctg aagcagaagc ctacaaaggg caacccggcg gcgctctccc cctgatagca
    11401 ccttaacggg tgtatcgccc tccgggcacc ggagagcatc catagctctt tcaagtttcg
    11461 agtccaggtt ccaggcatcc agatgctcta acttgtcagt tagttcaccc tgtttttcaa
    11521 tgagtttgtt catctcatcg tcgctcatag gctctgcgaa gcgagcgttt accgcttcgt
    11581 attccttgag caggtcaact acttcctgaa ccccctcctc aacaacctca cggactgttt
    11641 tattttcatc cagttgaggt tcctgttcca ggtagcccac tgtgtagccc ggtgaaaata
    11701 ccacttcccc ttcgtacgat ttctcaaccc ctgcaataat cttcatcagg gtcgatttac
    11761 ccgaaccgtt taaaccaata ataccaatct tagcgccgta aaaaaacgac aggtagatgt
    11821 tgttcagcac tttcttgtgg ggagggaaag ttttactcac ccccaccatg gaaaagataa
    11881 ttttttcgtc ggccattgtg tttacatttt cagctgcaaa aataaccgcc ggcaggcaaa
    11941 tacccgtcgg cggtaaaaaa atattttcag attctactac ttattgattt ttgcaaagtc
    12001 cttggatagg aaaccatcgc cttttgattt ggatatggta tgcatatcgt agtagtagag
    12061 ttggtcgtcg ccggcgccca gaatcatttt ccaaacacgc cactgcaggc gactgccttc
    12121 cgggccaacc ttatgcaggt aaagcacatt tgggttcttt tcctggatag ccttttgtat
    12181 atcctccggg tttacaacct tgatgttaaa cttgtaaacc ttcctaatct cagcaagagg
    12241 acgaaccgtt ttgtcgagtg cgctctctgt tacccaaagt tccttacttt ttacctgttt
    12301 catgttctta ttgtagaact taaaggcatc cttcttgatg agtttaggat tctcattaat
    12361 ggcggtgata tagttttgca tgaaacggat aataccctca agcttgtaag tgtatgtgtc
    12421 ctcggataaa tccttgtagc taagtggaac ggagcagata tcgggcatat cggtaatttt
    12481 tgaaacggca gcacccatcg atacgctcag gaaattgtag gtaggggcaa tcttgtcctt
    12541 gtcgaaccgc atttggatta gcaccaagaa tgacttgtcg atatccttgc gcatatcgtt
    12601 aaactcctta gcggtaatga acttgtaagg ggtgattttc catgaacgtt caacggcttc
    12661 acggatttcg atattaaatt gcgatgtagg attgttttcg agtaccacat aggttgttga
    12721 agccataaat gcctttacat cctcgggcct aaccaggtta cgttgtgcca tgccccatag
    12781 ggttatggtt accagtccga taataagtga tagcttcttc atgatcatct atttttatgg
    12841 tttttgtgtt tttctattgc cattcacaaa tttaatacac ttttcaatat tgataggttc
    12901 aaatctgaaa attccttcaa taacgactta atatttttaa aatcaataaa taaatgattg
    12961 tccctgatag gtttataggt aatttagaat ctaaaaaatg ttttttagca ttttttctta
    13021 cctttgcgca tggtggaaac gatatagcat ggtttaacct aacacaattc caatgacaga
    13081 aagtagaaat aagattgtca atgcggatga gatcaagaaa gctgtaaacc ttggcccaat
    13141 cattggtgat gggtttgccc gtttactgat gtacatactc cgattcaatg agcttaacaa
    13201 gaagtacgct aacgtggcaa accttcaggg gcttgaattt ctcaatgcag cgcttgatat
    13261 ccttgaggtt aagttcgagg ttaaccccga ggagctaaag cgaattccaa agaatggagc
    13321 gtttatttcg gtttcgaacc acccgtttgg tggaatggat ggtattttgc ttttaaaaat
    13381 tatgcttgag cagcagcgcg atgacattag gctgctctca aatttcttga tcaagcgtat
    13441 tcctcaggtt gaaaaatttg tattcgctgt tgaccccttt gaaaatggtg gttatcaaaa
    13501 aaattccaat ataagcgggt taaaagcggc ttacacacac ctatcggagg gtggtgcact
    13561 tggtcttttc cctgccggtg aggtgtcaac ctactacgac aacgttgatg aaaccggagt
    13621 aacagacagg cattggcaaa gatcaatgat taagttcatc aagaatgcac gcgttccggt
    13681 tgttcccatt tactttcagg gcgagaatag ccgcctgttc cacttgcttg gactaataca
    13741 cccgctactc cgtactgcca agttaccttc ggagttactt aacaaaaaga accaaaccat
    13801 cagggtgcgt atcggatacc caattagcgt tgaggaacag gataactact gcgatattga
    13861 gaaatttggt cggttcctta gagttaaaac atactcgttg ggatcaacca tcgatgtgga
    13921 taagttcttt aagtataagc taaaatcgga acctgaaccc gaacctatca ttcctcctgt
    13981 tccacaggaa gtgatagagg atgaggtttc aaaactgatt gaaaattacc tgttgttccg
    14041 tagcaaaaac tatgcggtta tttgcgcccc ttcagttgag atgcctaacc tgatgactga
    14101 aataggtcgc ctgcgcgaaa ttaccttccg cgatgtgggc gagggtacaa atcgaaaaat
    14161 tgacgtggac gagtttgacc tctactactg gcagctattt atttgggatg aggacgagcg
    14221 acgtattgtt ggtgcatacc gtgccgggaa agggaaagag ataattgaaa agtacggcat
    14281 tgaaggattt tacatccaaa cgctttttaa aattgataaa gcctttgccc caattcttga
    14341 gcagtgcatt gagcttggcc gttcgttcat tgtgcccgat taccaacgca agccgctacc
    14401 gttgttccta ctctggaaag ggattcttta cttccttatt aaaaacccgg agtaccgcta
    14461 ccttattggc cctgtaagta tatcgaaccg gttctctgat tactccaagg gtgtaattat
    14521 tagcttcatg aaatcgaact actacgacca tgactttgcc cgatttataa aatcgcgcaa
    14581 taaattcaag gtgcctatta aagatgagga gttcgatatc atttttaaca atagcgacga
    14641 tcttgggaaa ctcgataagt tcatccagga ggttgaacca tacgattacc gcatgcccgt
    14701 gctgctgaaa aagtatgtta agttaaatgg caaaataatt ggtttcaacg tcgatcctaa
    14761 atttaacaac gcgctcgatg gcctgcttat actcgatctt ttccaggtac ctgctgaaac
    14821 tatcacttcg ctttccaagg agatcaacga taaatcgata ctggaaaggt ttaacattac
    14881 cgactacacc tttgaggatc aggcagagta aaaaagaaca gaaaaggcag caaaagcgct
    14941 gccttttttt atgagtaaac ttttaaacta atggttagtg gtttagtcta ttaaaaaaaa
    15001 tccatatgaa cagatatttc ttaatcttct tagcttttgc ttttttgggg tgttccaatc
    15061 gtacagtaac cgatgatgca aagctcgaac agcgtgccaa agcacttcat tccaagatgg
    15121 ttaccattga tacccatacc gatacccctt taaattttct ccgtaccggc tttgacttta
    15181 ccggccaaaa caacaccgca attggcagca aggtggattt ggcaaaaatg gagcagggtg
    15241 ggttagatgc tgcctttttt gcggtattca ttggtcaacg cgagtgcaca cctgaaatgt
    15301 acgctcaggt taatgcaaaa gccaaagaga tatttgatgc tatttacact accgttaaca
    15361 agcacagtaa ccgtgcatca attgcaacta aacccgacga tgtgtatagg ttaaaaaagg
    15421 agggtaaacg cgccatttac ataggggttg agaatggtta ccccataggt gatgatctca
    15481 gccaggtaaa gctattctac gatatgggag cccgttacat caccctttgc catacacgga
    15541 ataaccagat atgcgactca tcaacggatc ctgatggccc acgccacaat gggctaagcc
    15601 aatttggccg cgaggtagtt gcggaaatga accgcctggg aatgatagtg gatgtgtcgc
    15661 acatatccga taaggcattc tacgatgtac tggaggtttc aaaggctccc gttattgcct
    15721 cccacagctg ctcccgcgcc atttgcgata atccccgcaa tttaaccgac gacatgctcc
    15781 gttccattgc aaggaatggg ggagtggtac agatgtgtat cctgagcgag tatgtaaaaa
    15841 agggtgaacc caaccccgct cgcgatagcg cttaccgtgc tcttcgtagg aagtggaata
    15901 acttcagggg cttaaccccc gaacaggaaa aacaggctac aacggagtgg tatgagcttg
    15961 aggagaaata cccggcatcg ttggctaccg tggccgatgt ggttgaccac atcgatcata
    16021 tggttaaagt aatgggaatt gaccatgtgg gaataggcac cgattttgat ggcggtggcg
    16081 gtgttgatgg ttgtgccgat gcctcgcaga tgtaccgcat aaccatggag ctcctccgta
    16141 ggggatattc cgaaagggat atccgtaaaa tctggggcga aaacttctta cgagttttcc
    16201 gcgaggtgga aaagaggaag gagcggggat aagaaaagca catatcgttc gaagaatcag
    16261 atgacatctc accaattggg agtcaatacc tttgtcttct tcactccctt tgtcatgctg
    16321 agcccagccg aagcatctca ttttactttc attccttcta ttgatattcc tcaaccaaca
    16381 aggaataact taagcattgg cataaaagcc tgacccgaca atgagtcgag cttttcagcc
    16441 ttgattttct ttggttcttt cttgtatcaa gacaagaaag aacggatata tcgctctttg
    16501 gcatgcgttc ccgttgcgat gcaacgattt agtgttgctc tctggaaaaa gtattgcgtg
    16561 catcgctata cgtgtggaag tcgtaatgga gttacaaatg tttagcccct acggggctgg
    16621 aaacgttatt cattgttcgt ggaattattg tcatgttgag cactagcgaa acatctcatt
    16681 agggtcgatt agagatcctt cgctgcgttc aggatgacaa gaagagttga tagttgatag
    16741 tgaaaaaaag catttcacga aacactaaaa accaaacacc actctccatc ttccctgtac
    16801 actgaacact atacactgca cactattcaa tatatccttc gcttcgctca gtatgacaaa
    16861 aggaaagcct gttttattat ttgtcgacat agattggtga tattaaacta tcaattaaaa
    16921 agtagccaat aatgagtaat attcagacta aacctgctta atcagtgcca tcagttttcc
    16981 attactcccg aacaacgagt aacgaatcac gaataacgaa attggtcgac cctcccgccc
    17041 acaggcggga tgcgcaaacc agcatgggaa aaatgaaaaa aggaagcggt ttgcttcctt
    17101 gtcaccagtc ggggtggtgc gactcgaacg cacgaccctc ccgcccacag gcgggatgcg
    17161 ctaaccaaca tgggaaaaat aaaaaaagga agcggtttgt ttccttgtca ccagtcgggg
    17221 tggtgcgact cgaacggtcg accctcccgc ccaca
//
LOCUS       DAOOWJ010000060        15390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3865_length_15390_cov_8.905902, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000060 DAOOWJ010000000
VERSION     DAOOWJ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15390
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3865_length_15390_cov_8.905902"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(567..2813)
                     /locus_tag="PLW22_07210"
     CDS             complement(567..2813)
                     /locus_tag="PLW22_07210"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOU74529.1"
                     /translation="MTGGFRRALTLGLLILTLSVKIFAQIPDGYYATAEGKTGAELKT
                     ALYNIIKNHTVISYDGLWTAFQYTDKKSNGKVWDMYSDKPGQTPPYEFTFVTDQCGNY
                     SSEGDCYNREHSFPKSWFNDASPMYSDLFHLYPTDGYVNNRRSNFIFGEVTSVTWTSL
                     NGSKLGTSSASGATLTVFEPIDEYKGDFARTYFYMATRYENLIATWASYNTEAKAILD
                     GTAYPAYKQWYITLLLKWNQQDPVSQKEIDRNNVVYSSYQHNRNPFIDHPEYAQLIWG
                     STTPITFTSTPITSATVGVTYTYNVTATGGNGTPLTISATQKPTWLTLTATGNGTATL
                     SGTPGQENVGGNQITLKATDGISETQQTFTITVSSPVTPIQFTSTPITTAVENQLYTY
                     NATATGGNGASLTITATQKPAWLTLTSTGNGTATLSGTPDHSNIGNSQVVLAATDGAN
                     QGTQSFTINVSAAGNSKPLITNVNITPASPVTRQAITISANITDSDGSVAKVLLGWGV
                     TVGNLSNTYEMQFENGAYSAPIASQTSPGTIYFQLAAIDNLQDTAYYNGSFTVNLNQV
                     PTISSVSHTPTNPTSADAVTVTANVSDPEGRIGNIFLLWGITESSLINQLQMSGTNGV
                     YTSVIPANPSQTSVYYRVKAYDAEGQQTLSSISSYIVNPATEIRTETSKQFLVFPNPF
                     NAFIQVEYPSAESYSVTFTNLIGQVVYSKTRVTGNLRIDCSDLQSGIYVLTIKSDTGI
                     VTQKMIKR"
     gene            complement(2826..3371)
                     /locus_tag="PLW22_07215"
     CDS             complement(2826..3371)
                     /locus_tag="PLW22_07215"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOU74530.1"
                     /translation="MSKNELKNQIRKAIKSHRLLLDPFLARERASYVFAEIESMEQFQ
                     KATTVLAFWSLPDEINTHDFVIKWAGSKRMVLPVVVGDELELRLFNGVENMEKSGGFG
                     IMEPKTGSIVNADEIDFAIIPGVAFDRNGNRLGRGKGYYDRTLPLLRNAVKVGIAYEF
                     QIIDSVPVSEHDIPVDLVVSN"
     gene            complement(3591..6431)
                     /locus_tag="PLW22_07220"
     CDS             complement(3591..6431)
                     /locus_tag="PLW22_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74531.1"
                     /translation="MFNKKIYFFTLILIILINNSKAQYYQTGVEPFKTDWKKREYKNI
                     RLIYPAEADSLARHYLSALAKSDSLNDVDYNLGTRKIDVVLHPNTVLSNGFVAWAPRR
                     MELVTQPDADGDALLWHKALSIHEMRHVKQMYALNSGSTKVLSWLFGQQAVGFASSFI
                     HPWIFEGDAVWAETNYTFSGRGRSATFFNHYYAHLVSKQKPYTYDKWLLGSYKDFIPN
                     HYQFGYQLVSFINQKWGSTTIPKTYRYVGRYPFTLFPTYLSLKKFTGQSRKSIYQKAF
                     SYNDSLWLVDYTEHYGNELPKAVNEDFKNLIHPYPLTDTTTIVYVTSLSDTPYFAVLD
                     KRGGIKKIVSTGILIGQPSYNDSLIVWAEYQPHARWEWMSSSAIKVYNIKTKRLNTIP
                     GGRYHSPIIDEDRIICIEYSSNGQYKLVGINFLGEKSTLLNFEGGYEVQQICLDTKGK
                     NIYGIAVSETGKKIFKVDSALTFDIVYNAAYRDIRTLACDGSNLFFTFTDGYTESIYK
                     YDIQNNIVYKIINLPFNSSYPTYKNHQLYYAHYTANGYRIAFLDDITSNRKIVDNISP
                     YNEIVKVNPTTVHDNYGIIQTEPTEYKGLNTLVNIHSWFPFYTKPLNDPANIEDESNI
                     YPGITLLSQNLTGSTLFNAGYGYGKTHLLFAEVTYNGLWPVFRLNIEKTDATASLYRV
                     TQFYPENRDYYKKAEFTSILPFTLGESIYSTSLQLYNKTIYSNSYLYRESIDAYRSGL
                     ITNEFGFTFYSLRRMAHRDLQPKSGLIINTGLLTTPWDYGNLPNLWYSQAKIYLPGLH
                     RNHGFGITLLAQKQNIKYIYLNNKVNFPAFYTSRPSTRFTGMYFNYIMPIAYPDLPIS
                     SLVYVKRISLNLFADFAKNSYKTYQQYELVTLTDNLQSIGFDAIVDFHLFRTWYPFRI
                     RFTQAFNGSDFNPYSNIVLTINLYSTFARPKL"
     gene            complement(6447..7706)
                     /locus_tag="PLW22_07225"
     CDS             complement(6447..7706)
                     /locus_tag="PLW22_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU74532.1"
                     /translation="MRTKIIIIGFLLFAYRLSLADTLTVMQYNLMYYDKEYSDCNATN
                     NNVDAKDGYLRTILNHVKPDILSVNEVNDAIASVERIKTNTLNYGGETRYKRANFSGS
                     FLVNMIYYNSEKLELKSQDAIATSPRETNVYKFYLKTSGLVNGDTIFLYHFVTHLKAG
                     SYESDAQQRAVAANSIMSYISTKNIKGNVILSGDMNLYSASEGAFVAFTTPVGSNNFR
                     FYDPLNAVGSWHSNYSYRYVHTQSTRTVASEGCFSAGGLDDRFDFILSSNDILAGTSG
                     IKFYSYKTLGQDGNRYNGSITSPTNTSIPTDVANALYGMSDHLPVVMKVTYSSAPTQV
                     NTEKNQVSILYNNPVYNTLNVQILENTSIKAINVYNLLGNCILNLIPESNSQKVDVDL
                     SGAPTGVLLVEVRLNDGRKKTIKVLKQ"
     gene            complement(7711..8049)
                     /locus_tag="PLW22_07230"
     CDS             complement(7711..8049)
                     /locus_tag="PLW22_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOU74533.1"
                     /translation="MDWKDKLNFAYSTNPDYKPNDEDDNTTREVVPPSKQRLIVGIER
                     KNRGGKCVTIVKGFVGSDDEIEKLGKTLKTRCGVGGTVKDGEIIIQGEIKQKVASILQ
                     ELGYRVTISG"
     gene            8306..11353
                     /locus_tag="PLW22_07235"
     CDS             8306..11353
                     /locus_tag="PLW22_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU74534.1"
                     /translation="MSNFRRANNIVGWIVFAISAIVYLLTIEPTASFWDCGEFIATAF
                     KLEVGHPPGAPLFMMIARLFALFAPDVTKVAALINSMSALASAFTILFLFWTITHLAR
                     KLIVGNSYATSTGQLIAILGSGAVGALAYTFSDTFWFSAVEAEVYAMSSLFTAFVFWA
                     ILKWEEQADEAHSTRWIIFIAYVMGLSIGVHLLNLLTIPAIAFIYYFRKFKTTRNGIL
                     TTLAVSAIILGSILYIIIPGTFKFGSWFDLLFVNSFGLPYNSGLFFYFVLLFGALAYG
                     IYYSHKKGRVILNTVLVGVTVILIGYSSYMMILIRAYANTPINENNPSNAFSLISYLN
                     REQYGDRPLFYGQYYNAPVTDSKDLETWIPEDGKYVRGYLKTKYEFDDRFKTLFPRMY
                     SNQPDHVAEYKKWANIQGKPVQVNTRDGGTETRYVPTFGENLLFFFRYQIGHMYIRYF
                     MWNFVGRQDDVQSHGGVENGNWISGIKPIDAIFLGNQDNLTDDMKSHPSRNTYYFLPL
                     ILGLLGIYFQLQVKKDKKNFWVVFMLFFLTGIAIVMYLNQYPLQPRERDYAYAGSFYA
                     FTIWIGLGVLAVYEWFRKKLSETPSAIAATTLCLVVPVIMGTENWDDHDRSGRYIARD
                     FAYNYLNSCEPNAILFTNGDNDTFPLWYAQEVEGIRTDVRIVNLSLLGTDWYIDQMKM
                     KCYQSAPVPISMKHDQYVMGTRDVVYIINRLNEPIELKQLMDFVLSDDPAKKYRIPGE
                     KPLDYLPTNKIKLTVDKSKVLSSGTVKEKDASLIVDTMEWTLKGQYITKSALAILDII
                     ANNNWERPIYFVSPFGDGDIGIADYLQHEGFAYRLVPIKSNSSDYLNISRIDSDILYD
                     RLMNRFRWGRINEPDVFIDHNVQRTAMVLKLRNSFSRLAKKLIEEGKKDSALKVLDRA
                     VELLPHPKFPYDFFMFGIIEGYYKLNEVQKANKLTLDYAKVSEQKLRYYVSLDDRMKQ
                     YYQDDIGLTAQTLQELASIAETYGQNEVAVDINKMIQAALTLK"
     gene            11424..12935
                     /gene="atpD"
                     /locus_tag="PLW22_07240"
     CDS             11424..12935
                     /gene="atpD"
                     /locus_tag="PLW22_07240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOU74535.1"
                     /translation="MSDNFGEVVQIIGPVVDVAFPSKEKLPSIYEALELTRPDNTILV
                     LECQQHIGESTVRTIAMDSTDGLSRGMKVRATGKSITMPVGNKIKGRLLNVIGETIDG
                     LPQVDRQSNYQVHSKPPKFDQLSTQKEILYTGIKVIDLLEPYSKGGKIGLFGGAGVGK
                     TVIIMELINNIAKGYSGMSVFAGVGERTREGNDLLREMIESDVIKYGKEFKESMLKGG
                     WDLSLVDTEELEKSQATLIFGQMNEPPGARATVALSGLTVAESFRDGGEDGQGRDILF
                     FIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKKGSITSVQAIY
                     VPADDLTDPAPATTFSHLDATTVLDRKIAELGIYPAVNPLDSTSRILTPEVVGDEHYN
                     TAQRVKNLLQRYKELQDIIAILGMDELSEDDKLVVHRARRVQRFLSQPFHVAEAYTGM
                     KGVMVSIKDTIKGFNMIMDGEVDQYPEAAFSFVGTIEEAIEKGERMLAEAKKQ"
     gene            12939..13196
                     /locus_tag="PLW22_07245"
     CDS             12939..13196
                     /locus_tag="PLW22_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOU74536.1"
                     /translation="MICELYSVEKFFQVEDVYYVQVPGTKGSFGILKKHAPIISSLEP
                     GVVKIVRNDGAELFFAIPAGGFVGVENDVISIVSEEINQTF"
     gene            13200..13934
                     /locus_tag="PLW22_07250"
     CDS             13200..13934
                     /locus_tag="PLW22_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOU74537.1"
                     /translation="MKKNAWLTILFAFILNLNETMAQQKISFPAKDGIEVTADLYFLA
                     SDKPYVILLHQARYSRGEYIEIAPKIVNLGYNCIAVDLRSGNEVNGVVNETAKRAKEK
                     GLPQNYTDAIPDIEAAIAYVKRQTDLPFVLWGSSYSASLALIQTAKDLRTKAVVAFSP
                     GEYFDTPEFVSSKIKDISVPVIVLSSKAECSSVKGLIEAIKRQNLVTQFCPTDKGKHG
                     SSALWKSNPSSNDYWLAITMFFGRLK"
     gene            complement(13935..15032)
                     /locus_tag="PLW22_07255"
     CDS             complement(13935..15032)
                     /locus_tag="PLW22_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU74538.1"
                     /translation="MRNKLLKEKRMRGYFIEAAKEILKGEGIDSMSVRNIADQAGYSY
                     ATLYNYFKDVTDVINECINDFAEECQEYVDEKTRNLPDGPEKLKAIIKSYVGYFLEYP
                     SIFDVFFLEKINKIEKKRDTSQLIVTLLERLCKPQWNYLINNEYISSSSAEKAITILR
                     YQIPGMLLFYLNRSNPDSPKEFYSLFDTQLDKLIRFEIPTRTTQTFEEVVLKFIFDGT
                     YPGENYYFFIHYTREKQVVDSILKTGFKYIESFHNSAEQIIDDKLDFLYKHNIYKPYG
                     NFIVVIGISRNIFDKYAQLIRSKGINTYIENILCDTAPEFDDEAEEYRYTLPTQYIKG
                     YVNYVTGETVKNPSFNPDYDSTNFLNNLNSL"
BASE COUNT         4395 a         3132 c         3179 g         4684 t
ORIGIN      
        1 accaaatgag tcacgccaca ggcgtggcga atagtatcgc gcggcagttc cacccacggg
       61 gtacccatcg ggtcgggctt ttcagccttg attttctttg gttctttctt gtatcaagac
      121 aagaaagaac gtttaaatag ctctttgccc tgctttccca ttgcgatgca acgattcacc
      181 cttgctcttt ggaataccat agcgtgcatc gcttaatatt ttgaatacct caataccctg
      241 ggttaaaacc cagggctaat gtgatttgcc cttttagggc gaataatgca cgcgtatgga
      301 gaagcacgct aacaagtggg gttacagata ttacgcccct acagggcggg aagcgttgtt
      361 ctttgttcgt taatcgttgt tcttgaagct actttcatgt tgagctaagc gaaacatctc
      421 aaggggggcg attagtaatc cttggctgcg ctcagaatga caggcatgta tatttttgtt
      481 aactaaacga cattgaaata gttactcaca ttttaatggg ttactaaaaa ctgcatctcg
      541 gttgctagta taaagccatt aaatgcttat cgtttaatca ttttttgagt tactatgcca
      601 gtatcacttt tgatggttag aacatagatt ccgctttgta aatcactaca atctattcgt
      661 aggttaccgg ttactctcgt tttactataa actacctgtc ctataagatt tgtaaaagta
      721 accgagtagc tttcagctga agggtattca acttgtataa aagcattaaa cggattggga
      781 aaaaccaaaa attgcttgct ggtttcagtt cgtatttcgg tagctggatt aacaatgtaa
      841 ctactaatac tgcttaaagt ctgttgcccc tcggcgtcgt aagccttaac cctatagtaa
      901 acgcttgttt gcgatggatt tgctggaata accgaggtat acactccgtt agtgccactc
      961 atctgtagct gattgatcaa actgctttct gttatacccc aaagtaggaa aatatttcct
     1021 attcggccct cagggtcgct tacgtttgcg gttacagtaa ccgcatcggc tgaagttggg
     1081 tttgttgggg tatgtgaaac gctagaaatg gttggtactt gattgagatt tactgtaaac
     1141 gatccgttgt agtaggcggt atcctgtaaa ttatcaattg ccgctagctg aaaatagatt
     1201 gttcccggac tcgtttgcga agcaatgggt gcactgtatg caccattctc aaactgcatt
     1261 tcgtaggtat tactcaagtt gccaacagta acgccccaac ctaagagaac ctttgcaacg
     1321 ctgccgtcgc tatcggttat gtttgcggaa atagttatgg cctgtcgggt tacaggcgac
     1381 gctggggtta tgttaacatt ggttattaaa ggctttgagt ttcctgcggc acttacattt
     1441 attgtaaaac tttgtgtacc ctggttggct ccatcggttg ccgctagaac tacctggctg
     1501 tttccaatat tactgtgatc gggggtgcca cttagggttg cagtaccatt tccggttgag
     1561 gttaatgtaa gccatgctgg cttttgtgtt gccgtaatgg ttaatgatgc cccatttccg
     1621 ccagttgctg tagcattata tgtgtaaagc tgattttcaa ctgccgtggt tataggcgtt
     1681 gaggtaaact gaataggggt aacaggcgaa cttactgtta tggtaaatgt ttgttgtgtc
     1741 tcactaattc catcggtagc ttttaaggtt atctgattgc cacctacatt ttcctggcct
     1801 ggtgtaccgc ttagggttgc agtaccatta cctgtggctg tcaaagtaag ccatgttggc
     1861 ttttgcgttg ccgaaatggt gagtggcgtt ccatttccac cggttgcagt aacattatat
     1921 gtataagtca ccccaacagt agctgaagta ataggcgtgc ttgtgaatgt aattggtgtt
     1981 gtacttcccc aaattaactg tgcatactct ggatgatcga tgaacgggtt gcgattatgc
     2041 tggtagctgg aatacactac gttattacgg tcaatctcct tctggcttac cgggtcttgc
     2101 tgattccatt tgagcagtaa agtaatatac cactgcttgt atgcaggata ggcggttcca
     2161 tcgagtattg ctttggcttc ggtattgtag cttgcccaag ttgcaattaa gttttcgtac
     2221 cgggttgcca tgtaaaagta tgtacgggca aaatcgccct tatactcgtc aatgggttca
     2281 aatactgtta atgttgctcc ggaagcagaa gaggttccta acttgcttcc attaagcgat
     2341 gtccaggtaa cagatgtaac ctcaccaaaa ataaaattgc tgcgcctatt gtttacataa
     2401 ccatcggtag ggtaaaggtg aaacaagtcg gaatacatgg gagatgcatc gttaaaccag
     2461 ctcttgggaa acgaatgctc acggttatag caatcgccct cggaactata gtttccacac
     2521 tggtcagtaa caaaagtaaa ctcgtaaggt ggtgtttggc cgggtttatc ggagtacatg
     2581 tcccatacct ttccattgct tttcttatcg gtgtactgaa aggctgtcca taacccatcg
     2641 tacgatatta ccgtatggtt ctttatgata ttgtacaaag cagtttttag ctcagcacct
     2701 gttttccctt cggccgtggc gtagtaacca tcaggaattt gtgcaaaaat ctttaccgat
     2761 agggttaaaa taagtaaccc gagtgtaagt gctctcctaa atcctccagt cataaaaatc
     2821 aacttttaat tgctaaccac aaggtcaaca ggaatgtcgt gttccgaaac cggaactgaa
     2881 tcgattattt gaaactcata ggctatgcct actttaactg cattgcgaag taaagggagg
     2941 gttctatcgt agtaaccttt ccccctgcca agcctattcc catttctgtc gaatgcaaca
     3001 ccaggaatta tggcaaaatc aatctcatcg gcgttaacta tactgccggt tttgggctcc
     3061 attattccaa aaccaccact cttctccatg ttttcaactc cgttgaataa cctcaactcc
     3121 aattcatcac caaccactac gggcaaaacc atgcgcttgc tgcctgccca ttttattaca
     3181 aagtcatgtg tatttatctc atccggtaac gaccaaaatg ctagaacagt cgttgctttc
     3241 tggaactgct ccatgctttc aatttctgca aatacataac tcgccctttc ccttgccaag
     3301 aaaggatcca gtaatagcct atgcgactta atagccttcc ggatttgatt ttttagctcg
     3361 ttcttgctca taaattttat tacggaatta acccagaaag gtttaatcca acactactca
     3421 aattatggag acattcaaat tttctaccca ataaccaatg ttgtttagat atcaaaacag
     3481 acattaccat cctgagtaaa tcagaagtat atctattttt ctaggttttg agatgtttcg
     3541 cttcgctcaa catgacaaaa gtccttaacc aaataacatt gaaaaaccaa ctacaatttt
     3601 ggcctggcaa aggtagaata taaattgata gttaaaacta tatttgagta aggattaaaa
     3661 tcggaaccat tgaatgcctg agtaaaccta atcctgaaag gataccatgt tctgaataaa
     3721 tgaaaatcaa caatggcatc aaaacctatg gactgtaaat tatcggttaa tgtaactaat
     3781 tcatattgct ggtaggtttt atagctgttc tttgcaaaat cggcgaaaag gttaagcgat
     3841 atacgcttta cataaacaag tgaacttatg ggtaaatcag gataagctat gggcattatg
     3901 taattaaagt acatacctgt gaatcgtgtc gaaggtcttg aagtgtaaaa agcaggaaaa
     3961 ttaaccttgt tatttaggta tatatactta atgttctgtt tttgtgctaa tagtgtaatt
     4021 ccaaatccat gatttctgtg caatccaggt aagtaaattt tggcttgact ataccacaaa
     4081 ttcggtaggt tgccgtaatc ccaaggtgtt gtcagtaatc ctgtattaat aattaagccc
     4141 gatttaggct gtaaatccct atgggccatt cgtcgtaacg agtaaaaagt aaaaccaaac
     4201 tcattggtaa ttaagcctga acgatatgca tcaatactct cgcggtatag ataactgttg
     4261 ctataaatgg ttttgttata tagttgtaat gatgtactat aaatgctttc acctaaagta
     4321 aaaggtaaaa tagaagtaaa ctctgccttt ttatagtaat ccctattttc agggtaaaac
     4381 tgtgttactc ggtaaaggct tgcagttgca tcggttttct caatatttaa tctaaaaaca
     4441 ggccaaagtc cattgtaggt tacctccgca aataacaagt gagttttgcc atacccataa
     4501 ccagcattaa aaagggttga tcctgttaag ttttgtgaaa gtaacgttat tccgggataa
     4561 atgttgcttt catcctcaat attggcaggg tcgtttagcg gctttgtgta aaatggaaac
     4621 cagctatgta tgtttaccaa tgtatttaac cctttatatt cggttggttc tgtttgaatt
     4681 ataccataat tatcgtgaac cgttgttgga tttactttaa ctatttcatt atatggtgat
     4741 atattatcta ctatttttct gttacttgta atatcatcta aaaaagcaat tcggtagcca
     4801 tttgctgtat agtgggcata atatagctga tgatttttat aggttggata cgatgaatta
     4861 aatggtaggt taattatttt atatactata ttattctgta tatcatattt atatatactt
     4921 tcagtgtaac cgtcagtaaa agtaaaaaat aggtttgagc catcacaagc aagtgttctg
     4981 atatcgcgat aagctgcatt gtaaacaata tcgaaagtta atgcagaatc aaccttaaat
     5041 atttttttgc cggtttcgct taccgcaatg ccataaatat ttttcccctt agtatctaaa
     5101 caaatttgct gtacttcata tcctccttca aaatttaaca aagtactttt ctctcctagg
     5161 aaatttatac caaccaactt gtattgtcca tttgagctat attcaatgca gataatccta
     5221 tcctcgtcaa taattgggga atggtatcgg ccacccggta ttgtgttcaa ccgctttgtt
     5281 ttaatattat aaaccttgat ggcgctactg ctcatccatt cccagcgcgc gtggggttgg
     5341 tattccgccc aaacaataag gctgtcgttg tacgatggtt ggccaattag tatgcctgta
     5401 ctcacaatct tttttattcc ccctctttta tccaaaacag caaaataggg tgtatcgcta
     5461 agcgatgtta catatacaat tgttgttgtg tcggttaacg gataggggtg tattaaattt
     5521 ttaaaatcct catttacagc tttaggcaac tcgttaccgt aatgttcagt ataatctaca
     5581 agccaaagcg aatcgttgta tgaaaacgct ttttggtaga tactcttcct tgactgacct
     5641 gtaaactttt tcagacttag ataggtagga aataacgtga atggatatcg accaacatac
     5701 ctgtatgttt tgggaatagt agttgatccc cacttttggt taatgaaact tacaagctga
     5761 tagccaaatt ggtaatggtt tggaataaaa tccttgtaag agcccaaaag ccatttatcg
     5821 taagtataag gtttttgctt tgatactaaa tgggcgtagt aatggttaaa aaaagttgct
     5881 gagcgacccc ttccgctaaa tgtataattt gtttcagccc aaacggcatc gccttcaaaa
     5941 atccatgggt gtataaacga tgaagcaaaa ccaacggcct gctggccaaa cagccacgat
     6001 aacactttgg tagatccgct attcagcgca tacatttgtt tcacatggcg catctcgtga
     6061 atggaaaggg ctttgtgcca aagcaatgca tctccgtcag catcgggttg ggttacaagt
     6121 tccatcctgc gcggggccca ggcaacaaat ccgttggata gaacagtgtt tgggtgaagc
     6181 acaacatcta ttttccttgt ccctagattg taatcaacat cgttaaggct atcggattta
     6241 gctaaagcgg aaaggtaatg tctggctaag ctatcggctt cggcaggata tattaacctg
     6301 atatttttgt attctctctt tttccaatcg gttttaaatg gctctactcc tgtttgatag
     6361 tactgtgctt ttgaattatt tattaaaatt atcagtatta aagtaaaaaa gtagattttt
     6421 ttattaaaca tattgattat catcatttat tgctttagca cttttatagt ttttttccta
     6481 ccgtcattta accttacctc aactagtaaa acaccagtag gagcacctga aaggtcaacg
     6541 tcaacttttt gactattgct ttcagggatt aagttaagaa tgcaattacc caacaggttg
     6601 tatacattta ttgccttgat tgatgtgttt tcaaggattt ggacgttaag ggtattgtaa
     6661 accggattat tgtacagaat acttacctga tttttctcgg tatttacttg agtgggagcc
     6721 gatgaataag tgaccttcat cacaacggga aggtgatcgg acatgccgta tagtgcattt
     6781 gccacatccg ttgggatgct tgtattagtg ggcgatgtaa tggaaccgtt gtatcgattc
     6841 ccatcctggc caagggtttt atagctataa aatttaatac cgctggtacc agcaagaata
     6901 tcgtttgagg aaagaataaa gtcgaaacgg tcatccagcc ctccggctga aaagcagccc
     6961 tcgctagcaa ctgttcgggt cgattgagta tgaacatagc gatagctata gttcgagtgc
     7021 cagctgccaa ctgcatttag ggggtcgtaa aagcggaaat tgtttgaacc aactggcgta
     7081 gtaaaagcca caaatgcgcc ctcagaggcg ctgtataggt tcatatcgcc cgagagtatt
     7141 acgttcccct taatgttttt ggttgatatg tagctcatta tggaattagc cgctactgcg
     7201 cgctgctgtg catcggattc gtaacttcca gctttgagat gggtaacaaa gtggtaaaga
     7261 aaaattgtgt cgccatttac caaacccgat gttttaaggt aaaacttata aacgtttgtt
     7321 tcgcgtggcg atgtggctat tgcatcctgc gacttcagct ccagcttttc agaattatag
     7381 taaatcatat taaccagaaa gctgccgcta aaattagctc gtttgtagcg ggtttcgcca
     7441 ccataattaa gggtatttgt cttaattcgt tcaacgctgg caatggcatc gtttacctcg
     7501 tttacggaaa ggatatcagg ctttacatgg tttagaatag tgcgcagata gccatcctta
     7561 gcatcaacat tgttatttgt agcattacag tcgctgtatt ccttgtcgta atacatcaag
     7621 ttatactgca taacggtaag tgtatcagct agtgatagcc gatatgcaaa aagcaaaaat
     7681 cctatgataa taattttagt acgcataaac ctatcctgaa atggtaaccc tataacccag
     7741 ttcttgcaaa attgaggcca ctttttgttt tatttcgcct tgaataatga tttcgccatc
     7801 ctttacggtt ccgccaacac cacaccttgt ttttagtgtt ttgcctaact tttcgatttc
     7861 atcgtcggaa ccaacaaacc ctttcacaat ggtaacacac ttccctcccc tattcttgcg
     7921 ttcaattcct acaattagcc gttgtttcga tggagggaca acttcccggg tcgtattatc
     7981 atcttcatcg ttgggcttat agtcagggtt ggttgagtag gcaaaattta gtttatcctt
     8041 ccaatccata tttttaaaat ttacaatata caaacatagt ccattttacg attattttaa
     8101 agtagaggtt gcctaacaga ttttacagct catccagtat tttattgaat taggataaaa
     8161 accaacaaga aaggcagata taaaaaaggg taattttagc gtaaggctta aaaactgcaa
     8221 attggtaatc agggtaaaac aaaatattct acatttgctt tcttgtaagc tgcaataaac
     8281 aattgtcaaa ataaaaaaat aaatcatgag caatttccgt agagccaaca atattgtcgg
     8341 atggattgtg ttcgcaatat cggcaatagt ttacctgtta accatagaac ccacagcaag
     8401 tttttgggac tgcggtgagt ttattgccac ggcattcaaa ctcgaagttg gtcacccccc
     8461 aggggcacca ctttttatga tgattgcccg cctttttgct ctctttgcgc cagatgtaac
     8521 aaaggttgca gcgcttatta attccatgtc ggctttggca agcgcattta ccatactttt
     8581 ccttttctgg actataaccc accttgcccg caagctaatt gttggtaata gctacgcaac
     8641 ttccactggt cagctaattg ccattctggg tagtggagca gttggcgccc ttgcctatac
     8701 tttttccgat accttttggt tctctgccgt tgaggcagag gtttacgcca tgtcgtcgtt
     8761 atttacagct tttgttttct gggcaatcct taagtgggag gagcaggccg atgaggcgca
     8821 ctcaacccga tggataattt ttatagctta cgtcatgggt ttatcaattg gcgttcatct
     8881 gctaaatcta cttacaatac cggccatagc atttatttac tacttcagaa agttcaagac
     8941 tacccgaaac gggattctaa caacccttgc agtatcagca attattttag gtagtattct
     9001 atacataatt attcccggaa cctttaagtt tggctcatgg ttcgaccttc tttttgttaa
     9061 ctcattcggt ttaccctata actcaggttt gtttttttac tttgttttac tttttggagc
     9121 ccttgcctat ggtatttact atagccataa aaaaggtagg gttattctta ataccgttct
     9181 ggtaggtgta accgtaatac taattggcta ctcatcgtac atgatgatac taataagggc
     9241 ctatgccaat acacccatta atgagaataa tccatcgaac gccttttcgc ttattagcta
     9301 cctgaaccgc gaacagtatg gcgatagacc attgttctac ggacagtact acaatgcccc
     9361 tgttactgac agtaaggact tagaaacatg gatacctgaa gacggtaagt acgtaagggg
     9421 gtacctgaaa acaaagtacg agtttgacga taggttcaag actcttttcc cacgtatgta
     9481 tagcaaccaa cccgaccatg tggcagaata caagaagtgg gctaatatac agggaaaacc
     9541 cgttcaggtg aacacccgtg acggtggaac cgaaacccga tatgtgccaa cctttggcga
     9601 aaatctttta ttcttcttcc gctaccagat tggccacatg tacattcgtt acttcatgtg
     9661 gaactttgtg ggccgtcagg atgacgttca gagccatgga ggggttgaaa acggaaactg
     9721 gattagcggt atcaaaccta tcgatgccat tttccttgga aaccaggaca acctgaccga
     9781 cgatatgaaa agccacccat cgcgtaatac ttactacttc ctccccctaa tattgggttt
     9841 gctgggtata tacttccagc ttcaggttaa aaaggataag aagaattttt gggttgtatt
     9901 catgctgttc ttccttacgg gaatagccat cgttatgtac ctgaaccaat acccgcttca
     9961 accgcgtgag cgcgattatg cctatgctgg ttcattctac gcatttacca tttggattgg
    10021 tcttggtgtt cttgctgttt atgagtggtt ccgtaaaaaa ctttccgaaa caccatcggc
    10081 aattgctgct acaacccttt gcctggttgt gccggtgatc atgggaaccg aaaactggga
    10141 cgatcacgac cgttccggtc gatacattgc acgcgacttt gcatacaact acctaaactc
    10201 ctgcgagccc aatgcaatcc tgttcaccaa tggcgataac gatactttcc cgctttggta
    10261 tgcacaggag gtggaaggta tccgtaccga tgttagaatt gtcaacctta gcttgctggg
    10321 taccgactgg tatattgacc agatgaaaat gaagtgctac caatcagcac ccgtgccaat
    10381 ttccatgaaa cacgaccagt atgtaatggg aacccgcgat gtggtttaca ttataaaccg
    10441 gctaaacgaa ccgattgagc taaaacagct catggatttc gttttaagcg atgatcctgc
    10501 aaaaaaatat agaatacctg gcgaaaagcc cctcgattac ttacctacta acaagataaa
    10561 gctaactgtt gataagtcta aagtgctgtc gagcggaacc gtaaaggaaa aggacgccag
    10621 cttgattgtc gataccatgg aatggacgct caaagggcaa tatattacta aaagcgcctt
    10681 agccattctt gatatcattg caaacaataa ttgggaacgc cccatttact ttgtttcacc
    10741 ttttggtgat ggtgatattg gcattgccga ttaccttcag cacgaaggct ttgcctacag
    10801 gctggttcca ataaaatcga actcatcgga ctacctgaat atttcaagaa ttgatagcga
    10861 tattctttac gataggttaa tgaaccgatt ccgttggggc agaataaatg agcccgatgt
    10921 gtttattgac cataacgtgc aacgtactgc tatggtttta aaacttcgta acagcttttc
    10981 gcgcctggcc aaaaaactaa ttgaggaggg taaaaaggat tctgctctta aagtacttga
    11041 cagggcagtg gaacttcttc ctcatcctaa attcccttac gatttcttta tgtttggcat
    11101 tattgagggt tactacaagc tgaacgaggt tcaaaaagcc aataagttga ccctggatta
    11161 tgcaaaggtt agcgaacaaa aacttcgcta ctacgtatca ctcgatgata ggatgaagca
    11221 atactaccag gacgacatag gcctaacagc tcaaacccta caggaactgg catcaattgc
    11281 tgaaacctat ggacaaaacg aggtggctgt tgacataaac aaaatgatac aagcagcctt
    11341 aactttaaag taaatttata gttgattttg ttagaatttg caacgggaaa ttcataaatt
    11401 tgaagtaagc aaaaagcaaa agtatgtcag ataattttgg tgaagttgtt cagattatcg
    11461 gtccagttgt tgatgtggcc tttcccagta aggaaaagtt accctcaatt tacgaagctc
    11521 ttgagttgac ccggcccgat aacaccatac tggttctgga gtgccagcaa catattggtg
    11581 aaagtactgt taggaccatt gctatggact caaccgatgg cctatcgcgc ggcatgaaag
    11641 tacgagcaac tggcaagtcg ataaccatgc cagtgggcaa caaaataaaa ggccgtttac
    11701 taaatgtaat tggcgaaacc attgatggtc ttccacaggt tgaccgccaa agtaactatc
    11761 aggttcacag caaaccacca aaatttgacc agctaagcac ccaaaaggaa atactatata
    11821 cgggaattaa agtaattgac ctgcttgaac cctactcaaa gggaggtaaa attggattat
    11881 ttggtggtgc aggagtaggt aaaacggtta tcatcatgga actcatcaac aatatcgcca
    11941 aggggtatag cggtatgtca gtttttgctg gggttggtga gcgcacccgt gaagggaacg
    12001 acctactcag ggaaatgatt gaatccgatg taataaaata cggaaaagaa tttaaggaat
    12061 cgatgctaaa aggggggtgg gacttgagtt tagttgatac cgaagaactt gaaaagtcgc
    12121 aagccactct gatattcggt cagatgaatg aacctcccgg ggcacgtgcc acggttgccc
    12181 tttccggatt aacggttgca gaatcgttcc gcgatggtgg agaggatggc caggggcgcg
    12241 atatactttt ctttatcgat aatattttcc gctttaccca agcaggctcc gaggtatcgg
    12301 cactgcttgg ccgtatgcct tcggctgtag gctaccaacc cacgctggct accgaaatgg
    12361 gtttaatgca ggaacgtatc acctcaacca aaaaaggttc tattacatcc gtacaggcca
    12421 tctacgttcc cgccgatgac cttaccgacc ccgcgcctgc aactacattc agccaccttg
    12481 atgcaaccac tgtacttgac cgtaaaatag cagagctagg gatttatccc gcggttaacc
    12541 cactcgattc cacatcgcgt atcttgaccc ccgaagtggt gggcgatgaa cactacaaca
    12601 cggctcaaag ggttaaaaac ctgcttcaac gctacaagga gttgcaggat atcattgcca
    12661 ttctgggtat ggacgaactt tcggaagacg acaaactggt ggttcaccgt gctcgccgtg
    12721 ttcagcgatt cctttcgcaa cctttccacg tggccgaggc ctatactggt atgaaagggg
    12781 tgatggtcag cattaaggat accatcaaag gatttaatat gattatggac ggcgaggttg
    12841 accaataccc cgaagcagcg tttagctttg ttggaaccat tgaggaggca atagagaaag
    12901 gcgaacgaat gcttgccgaa gcaaaaaaac agtaggttat gatttgcgag ctctactccg
    12961 ttgaaaagtt tttccaagtt gaggatgttt actacgttca agtacccggc acaaagggtt
    13021 cgtttggaat actgaaaaag catgccccta ttatttcgtc gcttgaacct ggagtagtta
    13081 aaatagtcag gaacgatggc gctgaactat tctttgccat acctgccgga ggatttgtgg
    13141 gtgtagaaaa tgacgtgatt tcaattgtat cggaggagat taaccaaacc ttttagtcca
    13201 tgaaaaaaaa cgcttggtta actatactct ttgcgttcat tctgaatttg aatgaaacta
    13261 tggcacagca aaaaatatcg tttccagcaa aggatggcat agaggttact gccgatttgt
    13321 attttttagc ctctgataaa ccatacgtta ttcttttaca tcaggcccga tatagtaggg
    13381 gtgaatatat tgaaattgct ccaaaaattg ttaacctcgg ttataactgc atagctgttg
    13441 atttgcgatc gggcaatgag gttaacggcg ttgtgaacga aacggccaaa agggcaaagg
    13501 aaaagggctt gccccagaac tacaccgatg ccatacccga cattgaggcg gctattgcat
    13561 acgttaaaag gcaaaccgat ttaccctttg tgctctgggg tagttcatac tcagcatcgc
    13621 tagctcttat tcaaaccgcc aaggatttgc gcaccaaagc cgttgttgca ttttctccag
    13681 gcgagtattt tgatacacct gagtttgtgt cgtcaaaaat taaggatatt tctgttcctg
    13741 taatcgtgct ttcatctaaa gcagagtgct catccgtaaa ggggttaatt gaagctatca
    13801 aacgacaaaa cctggtaacc caattttgtc ccaccgataa agggaaacac ggttcaagcg
    13861 cactctggaa atcgaaccca tccagcaacg attactggtt ggccattact atgttcttcg
    13921 gtaggctgaa ataattagag cgagttcaga ttattcaaaa agtttgtaga atcataatcc
    13981 ggattgaaac tcggattttt aacggtttca ccagttacat agttaacata acccttgatg
    14041 tactgggttg gaagagtgta cctatattcc tcagcctcat cgtcaaattc cggggcagtg
    14101 tcgcacaata tattttcgat ataggtatta atacccttgc tacgaattag ctgtgcatac
    14161 ttatcaaaaa tatttctcga aataccaata accactataa aattcccata gggtttataa
    14221 atgttatgct tataaaggaa gtcgagcttg tcatcaataa tttgttctgc tgaattatgg
    14281 aacgattcaa tatacttgaa accagtttta agaattgaat caactacctg cttttcacgg
    14341 gtataatgaa taaagaaata atagttttcg ccagggtaag tgccatcgaa aataaatttt
    14401 agcactactt cttcaaatgt ttgtgtggta cgggttggta tttcaaatcg aattagctta
    14461 tcgagctggg tatcaaatag cgagtagaat tccttagggc tgtcggggtt gctcctgttt
    14521 aggtagaata aaagcatgcc cggaatttgg taccgaagaa ttgttattgc cttctcagca
    14581 ctactcgacg atatgtactc attattaatc aggtagttcc attgtggttt gcataaccgc
    14641 tcaagaagtg taacaattag ctgcgaggtg tcccttttct tttcaatctt attgattttt
    14701 tcaaggaaaa aaacatcgaa aatgctgggg tactccaaaa agtatcccac ataactttta
    14761 attatggctt taagcttttc aggcccatcg ggcaggtttc gggttttttc atcaacatac
    14821 tcctgacact cctccgcaaa gtcgtttatg cactcgttga ttacatcggt aacatcctta
    14881 aagtagttat atagggttgc ataggagtag ccagcctggt cggctatgtt acgaacgctc
    14941 atactatcga tgccctcccc cttaaggatt tccttagccg cttcgatgaa gtagccacgc
    15001 atccgcttct ctttaaggag tttattacgc atcgatttac tgaattttgt acaaatataa
    15061 ttaacgctgt taatttattt aacaatgtta actaaaaaag atcaaacaaa tactattttt
    15121 ccctaaaagt aaagtttcag aagctttctt tttaattaag ttttgaagaa aaatacttta
    15181 tgttgattct ccaaaaagct taaatacttc agcgataaaa catttattgc ataaggtaga
    15241 atctgtaaac tgacttttct cattgcattt cggtgtaaaa aaacttattt ttgtttggaa
    15301 atgactaagt ggcacacatg agcagcaaac cgcttcatac gctttaccac aacaacggca
    15361 agggtctttt tgcctgctgg atttgctgat
//
LOCUS       DAOOWJ010000061        15266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3912_length_15266_cov_8.199461, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000061 DAOOWJ010000000
VERSION     DAOOWJ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15266
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3912_length_15266_cov_8.199461"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1541
                     /locus_tag="PLW22_07260"
     CDS             78..1541
                     /locus_tag="PLW22_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOU74539.1"
                     /translation="MNDKIIKIWEPISFTENWTGIDTSLFDELALSWYQKRKEFKEGN
                     KDYEDFINKLKRQHAIETGIIEKLYDLNEGITQTFIKEGFIESYISHEDTNITPSQLM
                     GYLKSHFEAMDFVFDLVKNDRQLTKSFILELHQLLLKHQDYTTAIDSLGNIVQVKVLK
                     GKFKEHPNNPKREDGTTFEYCPPIHVESEMDNLIKIYNDLEDKKIHPIIIATWFHHAF
                     TQIHPFQDGNGRMARLLTSLILIRHQLFPLNVLRNEKPEYIKALEEADNKKPERLVKF
                     FSKIQRRNIEFALNIQTKPDTLYEVAEIFKEKVEKITTEKKKERIEHIEKNWNLLSKN
                     VYSIIGKIQEELFNVVPKDKAYIQAISSKSNNYYYYTKQIIEYANEHNYFFNKNLKRK
                     WYRISFHVTKDKRYDIVISIHHFGYFDSVVAIGAFIEFIDKIHKDNRIEQTIPLNIKP
                     YTISLEKELTEASKNNIEEYLRDIFKIGMSIIINELS"
     gene            1592..2197
                     /locus_tag="PLW22_07265"
     CDS             1592..2197
                     /locus_tag="PLW22_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74540.1"
                     /translation="MKLKNSAREQAREPLCGIIIPYIIETKLIIQKIDNNRSMKKIIS
                     IISFIVLFSCKEKKEYQLQIIVKNKTNHTLTFKLFPKQEYMSGELYDFSEIGSGHRNT
                     EFQLEANAEMELFIVDELNVEPHTLTQKVFDSIYVTSIYNHHLIKFSPQSVYGYSENL
                     YTENSNWTLEHRNYDLPTNFQKSHVESSDYIFCVFEDKFIK"
     gene            complement(2365..4446)
                     /locus_tag="PLW22_07270"
     CDS             complement(2365..4446)
                     /locus_tag="PLW22_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOU74541.1"
                     /translation="MLRCLNTFRYFGIIAIAIIFCGSVRTYAQYYTIQKYSAEGGVKA
                     SGDEQWARAGERLEHPFIVVVTDSSGNPAPGVRVTFEITRVPFGSKGHLLERYEVFTN
                     SQGIARTQLHLGNKEGEYQISASIQTESPQNFVIFTAYANARNWAIMLIVGLLGGLAI
                     FFIGINQMSTGLRHGIADKLRLILLRMSRNRVVATFFGALVTVITQSSSATTVMLVGF
                     VQSGLLRFEQTIGMILGAAIGTTITVQLISFKLSDYSLLIVALGFGIMVFVKKHVTRS
                     IGEALVGFGLLFYGMHLMSGAMSPLKHYEPFLQVIESVQQPLIGVLVGTILTALVQSS
                     AAFIGIVLTMTTQGLISLEGSIPLLMGANLGTAVTGIIAAFAAGREAKKVAMAYAVFK
                     FIGILLLFWLIEPFAQLVRFLTPDVINTFAGGTVTNISRQVANAHTIYNVLLALIVLP
                     FTAQFARIVDWVMPIFPEKQQRYKTQYIDINLINTPSIAIEIARRELVGMAHLVNSML
                     SDSITLFTHKDSRVIDTIREKEEHVNFLRDKLNDYLLRISHADISDNEVDNVFRVMFA
                     TKELEQIGDIISKNLIEKGEWWLGAGLEFSDEGKNELVEFHRLTQKQLNRAIEILDTL
                     NLDVARKQLRKYDHYKKVGIELERNHFDRLKQAVGKSIESSKTHLELVSLYRIIGSHA
                     NNIARITIGEG"
     gene            4816..5514
                     /locus_tag="PLW22_07275"
     CDS             4816..5514
                     /locus_tag="PLW22_07275"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU74542.1"
                     /translation="MRNYFVVGASSGIGKALSIVLADSGNLVYGTYNKTPVPPETGNI
                     KYYPLDVMDDTINLEFLPEKLDGIAYCPGAITLKPFERIKSTELLSDFQLQVIGAVKL
                     LQAAMPRLRNGNYPSVVLFSTVAVQTGLPYHAVVSVSKGAVEGLTRALAAEYAPKIRV
                     NCIAPSLTDTPLASSLLNNEQKRDSANQRHPLKRVGMTEDIAYMAEFLLSEKSGWITG
                     QVIHVDGGFSTVKL"
     gene            5547..6002
                     /locus_tag="PLW22_07280"
     CDS             5547..6002
                     /locus_tag="PLW22_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74543.1"
                     /translation="MTLYDKYIKVEQAIAKGMNRWSIPAIRISFAIIFIWFGILKPFD
                     VSSAEALLKATVAWLPFGTPEFWLVVIGWWEVAIGVTFLFPKTTKIAIILLFTQMVGT
                     FMPLFFLPQVTFQNGNILTPTMEGQYIIKNVMIISAALVLGGRYYKGKE"
     gene            6102..6572
                     /locus_tag="PLW22_07285"
     CDS             6102..6572
                     /locus_tag="PLW22_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOU74544.1"
                     /translation="MAFYQLIRTQKLPVSTSEIWDFISSPLNLKEITPPYMGFAITGI
                     SGNGKMYEGMIISYRVSPVAGIKTNWVTEITHVKEGSYFVDEQRIGPYAMWHHEHRLE
                     PIEGGVLMTDIVTYQPPLGFIGALANSIFIKKQLQRIFDYRSVALEQRFGKYIP"
     gene            6569..7996
                     /locus_tag="PLW22_07290"
     CDS             6569..7996
                     /locus_tag="PLW22_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU74545.1"
                     /translation="MKILLTGATGYIGQRLLPVLLKNGHEVVCCVRDKNRFNIKKYSN
                     QKIKVIEVDFLKKESLIQIPDDIDVAYYLIHSMSTQSGNFQELEVISATNFKERLEQT
                     NVSQVIYLSGISNADELSKHLASRRNVETILSGSKFALTTLRAGIIVGSGSASFEIIR
                     DLVEKLPVMIAPKWLNTRCQPIAITNVIEFLVGVTGRPETYNKAFDIGGPDILSYKDM
                     LLRFARVRGLRRSIYIVPVMTPKISSYWLYFITSTSFSLAQNLVNSMKVEVVCQPNNL
                     TCMLGIKLIDYDTSIRMAFDTIEQNQVISSWKDAQTSNAISKGLGNYIEVPTHGCFKD
                     IRSRKVDSIDAAIKRLWAIGGKNGWYFGNFLWEIRGFIDQLAGGVGMRRGRKSDTDLN
                     PGDALDFWRVLIADKEEMRLLLYAEMKLPGEAWLEFRIDGNGILTQTATFRPLGLLGR
                     LYWYSVLPFHYLIFSRMLKRIAVDQ"
     gene            complement(8103..9440)
                     /locus_tag="PLW22_07295"
     CDS             complement(8103..9440)
                     /locus_tag="PLW22_07295"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOU74546.1"
                     /translation="MSKILVLGAGLSSSDLIKYLLDHSKVLGWTVRVGDLSLETARQK
                     VNNHPNGQAIQFDINNNKQLQDEVSWATAVISLLPAFMHPVVAQECVRQGKHMLTASY
                     VSDTMKSLHEEARQKGIALLNELGVDPGIDHMSAMRVIDEIRAKGGKLTGFISNTGGL
                     IAPESDNNPWNYKFTWNPRNVVLAGQGVAQYLENGQYKYTPYHRLFTSVREYNIMDLG
                     MFEMYPNRDSLKYREIYGIEDIQTIIRGTLRRAGYSKAWNVFVQLGMTDDTYKMANSE
                     KLTKREFLNSFLPYHPTETVEEKLKRIIPDAQDEVVFNKLKWLGMFDNEPIGLANASP
                     AQLLQHILEPKLALGPNDKDMIVMQHIFDYELSGKKHRKASTMVIIGKDTIHTAMSIT
                     VGTPLAIATKLLMTGQIKDRGVIVPTKPHVYNPILNELENFGVKFIEEDVELS"
     gene            9579..9803
                     /locus_tag="PLW22_07300"
     CDS             9579..9803
                     /locus_tag="PLW22_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOU74547.1"
                     /translation="MILLFVGLWELVVIVIVALLLFDSATLLNVARLLGRAYHYLQKS
                     IAEIRSQISLINLDDKDPENDDNPSSKHAG"
     gene            10054..10704
                     /locus_tag="PLW22_07305"
     CDS             10054..10704
                     /locus_tag="PLW22_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU74548.1"
                     /translation="MIKVESIVKSFGALTVLKGITVEIPERKVVAIVGPSGAGKTTLL
                     QIMGTLSQPDKGKVLYNGTDVTALNDTQLARFRNSHIGFVFQFHHLLPEFTALENVCM
                     PALIAGKSMTDASNRAKELLDFLGLSHRFGHKPSELSGGEQQRVAVARALINNPMVVL
                     ADEPSGNLDSHNKEELHRLFFHLRDTFGQTFVVVTHDKELARMADLQISMNDGQLV"
     gene            10707..11486
                     /locus_tag="PLW22_07310"
     CDS             10707..11486
                     /locus_tag="PLW22_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOU74549.1"
                     /translation="MLTPRSFAELADLLNNRYRRYACPAFIIGDPIQVPHGFSDKHDI
                     EISALFAATFAWGSRKSIVNSASKLMGIMGNKPYEFVVNFSKADEHALQGFVHRTFSS
                     ADAIEFVYVLRHIYTHLGGLEQVFAKGYRQQQSVHDAIQAYRNVFMECEVPARTLRHV
                     PNVAAGAAAKRINMFLRWMIRPATEGVDFGIWRTIPTSALLMPLDIHTGRVARKLGIL
                     QRAQDDFKAVVELTDALRQFDPADPVKYDYALFGLGLHEKF"
     gene            11498..12211
                     /locus_tag="PLW22_07315"
     CDS             11498..12211
                     /locus_tag="PLW22_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOU74550.1"
                     /translation="MGNSYFKFKQFTVHQSKAAMRVNTDGVLLGAWANVTDTNRILDI
                     GTGTGVIALMLAQRNTGAHIDAIEIDAQSAAEARSNVNLSPWANRIDVINCSLQSFAP
                     SVSHRYDLIVSNPPYFNQSLKSAHTGRNIARHTESLPYNHLIEGVIKLLAPSGRFCGV
                     FPYSEGNVFIALAGAHGLYCTQKVNVQSRPGRKVLRVLLQLETQKRAVAESTLTIHNP
                     DGSYSDEYKRLTEDFYLGF"
     gene            complement(12293..13264)
                     /locus_tag="PLW22_07320"
     CDS             complement(12293..13264)
                     /locus_tag="PLW22_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU74551.1"
                     /translation="MQNEIRKDNRTLKQIKSALFGVAVGDALGVPVEFKSRQELSQNP
                     VTTMIGYGTHNQPPGTWSDDSSLTFCLAEGLTNDFCLDEIGQNFVKWYNENYWTPNSE
                     VFDIGGTTKQAILRIANGENPELAGSSKETDNGNGSLMRILPLLFYLYNKPINVRFEI
                     TRKVSSITHRHIRSIIACFYYLEFAMQIIEGRDKFDIYSNLQTLVLNHLTNLSINPAE
                     ISLFDRLLKSSIYELDAEQIYSTGYVLHTLEASIWCLMTTSNFKEAVLKAVNLGYDTD
                     TTGAVTGGLAGLLYGFENIPEEWVLQIAKHHEIENLAERLCIKITSC"
     gene            complement(13452..14291)
                     /locus_tag="PLW22_07325"
     CDS             complement(13452..14291)
                     /locus_tag="PLW22_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOU74552.1"
                     /translation="MIPDSQTNTLYIADCLPKKHPKFFMDFEAILKKCGITFQLLPDT
                     KDIWAVDYMPVQVTVDKFVQFVYNPDYLQSRKWIKTITDVESICNSIRLNRIKSDIVL
                     DGGNVIKTTDKVIMCDKIFLENPNYSRRQLSDKLKELFEIDKLFFVPQQPKDFTGHAD
                     GMVRFLDNNTVIINDYSKEKPEFQRAFKIALDNTGLDYIEIPYNPYGNKTNGQANGIY
                     INFLQMQNTVIVPTFGIKEDDIAVRQFEQLFRGQQIATIDSNEVANNGGILNCITWNI
                     LTT"
     gene            complement(14292..15026)
                     /locus_tag="PLW22_07330"
     CDS             complement(14292..15026)
                     /locus_tag="PLW22_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74553.1"
                     /translation="MKDIKYLDLYLSSFELESKDEEVEQSKLAEFWHYLNGCPEIKSL
                     DEKFSTLAYYLMNYLCLVIADQPYRITELEVYYYDKDNHPDPYVHCATEQLFAGNWYF
                     NGAGLDITFGDYEKKIYGGLLIRGIMRFGDSPRYISGPSNVLKEIFSNIGNILTGKGS
                     ICLRELNKEKIQEIKTEPIQAVRIGLTKKKEDTENYAEKKYRYIVELNLQHKFKDKAK
                     VVRQLLADNKINKKQAKEIMGYDINP"
     gene            complement(15023..>15266)
                     /locus_tag="PLW22_07335"
     CDS             complement(15023..>15266)
                     /locus_tag="PLW22_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74554.1"
                     /translation="AQPATDSPTAVQPPPNTGRLLLFTASTQHKNICWEIRLVIWGKS
                     VNFTMGVGFWVNVKLSLYRDISNFHFLATKFYFERM"
BASE COUNT         4496 a         3172 c         3066 g         4532 t
ORIGIN      
        1 tctattgaac tttggtgctg aaaatccccc actgcggcaa tacccgaacc gttacaagca
       61 atttaatata acatatcatg aatgataaga taattaaaat ttgggaacca ataagtttta
      121 ctgaaaactg gacaggcata gatacttcat tatttgatga actagctctt agctggtatc
      181 agaaaagaaa agaatttaaa gaagggaata aggactacga agattttata aacaaactta
      241 aaagacaaca tgccattgaa accggtatta tcgaaaagtt atacgattta aatgagggaa
      301 taactcaaac attcattaaa gaaggtttta ttgaaagtta tataagccat gaggatacca
      361 atataacacc ttctcaattg atgggatatt taaaatctca ttttgaagct atggactttg
      421 tgtttgacct tgttaaaaat gatagacaat taactaaatc ttttatactt gaattacatc
      481 aactacttct gaaacaccaa gattatacaa ctgcaatcga ttcgttaggg aatatcgtac
      541 aagttaaagt tttaaaaggg aaatttaaag aacatcccaa taatcctaaa agagaagatg
      601 gaacaacttt cgaatattgt cctccaattc atgtcgaaag tgaaatggat aatttaatta
      661 agatttataa tgatttagaa gataaaaaaa tccatcctat aataatagca acatggtttc
      721 atcatgcctt tacacaaata cacccttttc aagatggaaa tggaagaatg gcaaggttgc
      781 taacaagttt aatactaatc aggcatcaac tattcccttt aaacgtctta agaaacgaaa
      841 agccagagta tattaaggca cttgaagaag ctgacaataa aaaacctgaa agattagtta
      901 aatttttcag taaaatacaa agaagaaata ttgaatttgc cttgaatatt caaacaaagc
      961 cagacacact ttatgaagta gctgaaatat tcaaagaaaa agttgaaaaa ataacaacag
     1021 aaaagaaaaa agaaagaatt gaacatattg aaaagaattg gaatctttta tcaaaaaacg
     1081 tttatagtat tattggtaaa atccaagaag aattatttaa tgttgtacca aaagataaag
     1141 catatattca agctatatca tcaaagtcaa ataattacta ttactataca aaacaaatca
     1201 tcgaatatgc taatgagcat aattattttt tcaataaaaa tttaaagaga aaatggtaca
     1261 gaatctcatt ccatgtaaca aaagataagc gatatgacat tgttattagt atccatcatt
     1321 ttggttattt cgattcagtt gtagctattg gtgcattcat agaatttatt gataagattc
     1381 acaaagataa tagaattgaa caaacaattc ctttaaatat taaaccctat acgatatcat
     1441 tagaaaaaga attaacagag gcatctaaaa ataatatcga agagtattta agagatatat
     1501 ttaaaatagg aatgtcaata attataaatg aattatcata aactgcatgt aacagcgtgc
     1561 ttgctccatg cctctgaaat taaaaaaatc aatgaaactc aagaattcgg cacgagagca
     1621 agcacgcgaa ccgttgtgtg gcataataat accttacatt attgaaacaa aattaatcat
     1681 acagaaaatt gataataata gaagcatgaa aaaaataatt tcaataattt cttttatcgt
     1741 tctattttct tgcaaagaaa aaaaggaata tcaattacaa ataattgtta aaaataagac
     1801 aaatcacacc ttgacattta aactttttcc caagcaagaa tatatgtctg gtgaattgta
     1861 tgacttttcg gaaatcggtt ctggacatag aaatactgaa tttcaactcg aagcaaatgc
     1921 agaaatggaa ctgtttatcg tcgatgaact taatgttgaa ccacacacat taactcaaaa
     1981 agtatttgac agtatttatg ttacaagcat ttacaatcat catttaatta aattttcacc
     2041 acaaagtgta tacggatact ctgaaaactt atacacagaa aactctaact ggacacttga
     2101 acatagaaat tatgatctac caacaaactt tcaaaaaagt catgttgaat caagcgatta
     2161 cattttttgt gtttttgaag ataaatttat caaataatta cgccacacaa cacgtggttc
     2221 aacctactgg ggctgatgat gcagattcga aagtttttag taatttagtg tgtttttaac
     2281 gtgggatgac gccgctggct tttaactccc cagcagccca aaccaccgac cgttacccat
     2341 cccaaattct aggttctgtt ttttctatcc ctcaccaatg gtaatccgtg ctatgttgtt
     2401 ggcgtggctg ccaattatgc ggtaaaggct caccagttca aggtgcgttt tggagctttc
     2461 aatggatttg cccaccgcct gcttaagcct atcgaaatgg ttacgttcta gctctatacc
     2521 aaccttcttg tagtggtcgt acttgcggag ctgcttgcgg gctacatcca ggttcagtgt
     2581 gtccagtatc tcaatggctc ggttaagctg cttttgggtt aacctatgga attcaacaag
     2641 ctcatttttc ccttcatcgg aaaactcgag tcctgcaccc agccaccact ctcctttctc
     2701 aatcagattc tttgatatga tatcgcctat ctgctccagc tccttggtgg caaacatcac
     2761 cctgaataca ttgtcaacct cattatcgct tatatcggca tgacttattc ggagcaggta
     2821 gtcgttaagc ttatcgcgca ggaagtttac atgctcctcc ttttcgcgga ttgtgtcaat
     2881 tactcttgaa tccttgtgag tgaaaagtgt aatgctatcg cttagcattg agtttaccaa
     2941 gtgagccatg cccaccagct cgcgccgtgc aatctcaatg gcaattgatg gagtatttat
     3001 caggttgata tcgatatact gggttttata ccgttgttgt ttctcaggga agattggcat
     3061 aacccaatcc acaattcggg caaactgagc ggtaaaaggc aaaacaatca aggcgagtag
     3121 cacattgtag attgtgtggg cattagccac ctgcctcgat atgtttgtaa cagttccccc
     3181 ggcaaaggtg tttattacat cgggagtgag gaacctaacc agttgagcaa agggctcaat
     3241 aagccagaat agaaggagta ttccaatgaa tttgaatacc gcgtaggcca tggccacctt
     3301 tttggcctcg cggcccgcgg caaatgctgc aattatcccg gttactgccg tgcccaggtt
     3361 ggcacccatg agtaagggta ttgaaccctc aagcgatatg agtccctggg tggtcatggt
     3421 tagcacaatc ccaataaatg ccgcactact ctgtacaagg gcggttaaaa tggttcctac
     3481 cagcacgcct attaatggtt gctgaacgct ttcaataacc tgtaggaatg gttcgtaatg
     3541 ctttagtggc gacatggcac ccgacataag gtgcatgccg tagaacagta ggccaaagcc
     3601 tacaagagct tcgccaatgc tgcgggtaac gtgttttttc acaaacacca taatgccaaa
     3661 gccaagcgca acaattagca gcgagtaatc ggataacttg aatgagatta gctgaacggt
     3721 tatggtagta ccaatggcag cgcccagtat catccctatg gtttgctcaa atcgcaacaa
     3781 acccgactga acaaatccaa caagcatcac cgtggttgcg ctactcgact gggttataac
     3841 ggtaaccagt gccccaaaaa aggttgccac aaccctgttc ctcgacatgc gtagcagaat
     3901 tagccttagc ttatcggcaa taccgtggcg taaaccagta ctcatttggt ttatgccaat
     3961 aaagaatatt gctaaccctc ccagcaagcc aacaattagc attattgccc aattgcgtgc
     4021 attggcatag gctgtaaaaa tcacaaagtt ttgcgggctc tcagtctgga tgcttgccga
     4081 tatttgatac tcgccctcct tattacccaa gtgaagttgg gtgcgtgcaa tgccctgact
     4141 gttggtgaat acctcgtacc tttccaaaag gtgtcctttt gagccaaagg gaacgcgggt
     4201 aatttcaaac gtaaccctta ccccgggggc aggattccct gaggaatcgg ttaccacaac
     4261 tataaatgga tgttcaagtc gctcaccggc tctggcccac tgctcatcgc ctgaggcttt
     4321 tacaccacct tcggcactat acttctgaat ggtgtagtac tgggcataag ttcttacact
     4381 accgcaaaat atgatagcta tggcaattat accaaaatac ctgaatgtat ttaaacatct
     4441 tagcatagtc caatttaccc aaaaatatcg agaatggcaa taggtttagt gttttttttc
     4501 aaaccatccc caaatcaatg cctgaactaa taatgagtaa tcccaaattc accaaatgaa
     4561 aaattaagtt gacgaatttc gtttaaaaaa gatttttcag tatgagaatg ttaatcaacc
     4621 cattaattct gggggagtaa atacactgtg cctctcgcta acgcatggac aattactcag
     4681 ctttacaaag ggtattataa actactccgc cttgcgcttt ttttgtattt tgagatttgt
     4741 aatactattt tttaaattac ttaaacaaaa atcttaaata tctgttatac tactaacaaa
     4801 acaaatcaac ggaatatgag aaactatttt gtagttggag cttcgtcggg gataggtaaa
     4861 gcgcttagca ttgttttagc cgattcgggt aacttggttt atggaactta caataaaaca
     4921 ccagttccac cagaaactgg caatattaag tattatcctc ttgatgtgat ggacgataca
     4981 atcaatctgg aattcttgcc cgaaaaactc gatggcattg cttactgtcc gggcgctatt
     5041 acgcttaaac cgtttgaacg cattaaatca accgaacttt tatccgactt ccaattacag
     5101 gtaattggag ctgttaagct tcttcaagct gcaatgccta ggctgaggaa tgggaattac
     5161 ccgtcggtgg tacttttttc cactgttgcg gtgcagactg gattacctta ccatgcggtt
     5221 gtttccgtat caaagggtgc ggttgagggg ttaaccaggg ctttggccgc tgaatatgcc
     5281 ccaaaaatta gagtaaactg tattgcccca tcgttaaccg ataccccctt agcatcatca
     5341 cttttgaaca atgagcagaa gcgcgattcg gctaatcaaa ggcacccttt aaagagggtt
     5401 ggaatgactg aggatattgc ttacatggca gagtttctgc tatccgaaaa atcgggctgg
     5461 attacgggcc aggtaataca tgttgatggt gggttctcaa cagttaaact ataagcataa
     5521 aaattaccaa ttaaactata cgaaatatga cactttacga taaatacatt aaagtagagc
     5581 aagcaattgc caagggtatg aacaggtgga gtattccagc tattcgtatc tcatttgcca
     5641 taatatttat atggtttggc atactgaaac cctttgatgt gtcgtcggct gaggccttgc
     5701 tgaaggcaac tgtggcttgg cttccttttg gaacacccga attctggttg gtggttattg
     5761 gatggtggga ggttgccatt ggtgtaactt ttctattccc caaaacaacc aaaattgcta
     5821 ttattttact tttcacgcag atggtaggaa catttatgcc gcttttcttt ttacctcaag
     5881 ttacctttca gaatggaaac atacttactc caaccatgga gggacaatac atcattaaaa
     5941 atgtgatgat tatttccgca gcattagtgc ttggtggccg atactacaaa ggcaaggagt
     6001 agtaccatac aatgaaaaag cgggaaaaaa tgaagaataa tttttcaagc ttgacacaat
     6061 tgcaagttta caatttaact aaactcaaca atccttaagc tatggctttc tatcagctaa
     6121 tcagaaccca aaaactacca gtttcaacca gtgaaatttg ggatttcatt tcatctccct
     6181 taaatttaaa ggaaattact cctccctaca tgggtttcgc cataacaggg ataagtggta
     6241 atggaaaaat gtatgaggga atgataatat cgtatagggt aagccctgtg gccggaataa
     6301 aaacgaactg ggtaaccgag ataacccatg taaaagaggg gagttacttt gttgacgagc
     6361 aaagaattgg cccatacgcc atgtggcatc acgagcacag attagaaccc attgaaggag
     6421 gcgtactgat gaccgatatt gttacctacc agcccccctt ggggtttatt ggagctttgg
     6481 ccaactcaat tttcataaaa aaacagctcc aaagaatttt tgattatcgc tctgtggcac
     6541 tagagcaaag gtttggaaaa tatataccat gaaaatatta ctcacgggtg ccaccggtta
     6601 cataggccaa aggcttttac ctgttctact aaagaatggg catgaggtgg tttgctgtgt
     6661 aagggataaa aaccggttca acatcaaaaa atactcaaac caaaaaatca aggtgattga
     6721 ggttgatttc ctcaaaaagg agagcctaat tcagataccc gatgatattg atgtagcata
     6781 ttaccttatc cattccatgt ccacgcaatc gggaaatttt caggaactgg aagtaattag
     6841 tgctaccaat tttaaggaac gactggaaca aaccaatgta agtcaggtaa tctatttaag
     6901 tggtatcagt aatgccgatg agctctcaaa acacttagcc tcgcgtagga atgttgaaac
     6961 cattctatcg ggttcaaagt ttgcccttac cacgttaagg gctggaatta ttgtgggttc
     7021 aggtagtgca tcctttgaaa tcattcgcga tttagttgag aaacttccag ttatgattgc
     7081 tcccaagtgg cttaatacta ggtgccaacc cattgcaatt accaatgtta tagagttttt
     7141 agtgggtgtc accggcaggc ccgagaccta caacaaagct tttgatattg ggggccccga
     7201 tattctcagc tataaggata tgctcctgag gtttgcaagg gtaaggggtt tacgacgaag
     7261 catctacata gttcccgtaa tgactccaaa aatttcatcg tactggttgt atttcattac
     7321 ctccacttcg tttagccttg ctcagaacct ggttaatagt atgaaagttg aagtggtttg
     7381 ccagcccaat aatttgacat gtatgctggg cattaaacta attgattatg atacttcaat
     7441 aaggatggcc tttgatacca ttgaacagaa ccaggtaata tcaagctgga aggatgctca
     7501 aaccagcaat gcaattagca aaggattagg caactatatt gaggttccca ctcatggatg
     7561 ctttaaggac atccgctccc gtaaagttga cagtattgac gctgccatta aacgcctgtg
     7621 ggcaataggg ggaaaaaatg gatggtactt tggtaatttc ctttgggaaa ttaggggctt
     7681 cattgaccag cttgctggcg gtgttggtat gcgtaggggc agaaaaagcg ataccgattt
     7741 gaatccaggc gatgcacttg atttctggag ggtattgatt gccgataagg aggagatgcg
     7801 attgttactt tacgccgaaa tgaaattacc gggtgaggca tggttggaat ttagaattga
     7861 tggtaacggc atactcaccc agactgcaac ctttaggccc ttgggtttgc ttggtaggct
     7921 ttactggtac tcggtgcttc cattccatta cctcattttt agtagaatgc ttaaaagaat
     7981 tgctgttgac caataacttc ccttcagtat taagaaaagt tttccgatac tctacttagg
     8041 ctataattac ttaaaaagcc ggacataaca tccggcttat ggttttaggt aatcgttaaa
     8101 atctagctca gctcaacatc ctcctcaata aatttaaccc caaagttttc aagctcatta
     8161 agaatcgggt tgtaaacatg cggcttggta ggaactataa cgccacgatc ctttatctgt
     8221 ccggtcatta gcaacttagt ggcaatggct agcggggttc ctacagttat agacatggcg
     8281 gtgtgaatgg tatccttccc aataatcacc atggttgatg ctttgcgatg ctttttgccg
     8341 gaaagctcat agtcaaatat gtgttgcatt actatcatat ccttatcgtt agggccaagg
     8401 gctaacttgg gctcaaggat atgctgaagt agttgggcag gacttgcatt ggcaaggcca
     8461 atgggttcgt tgtcgaacat tcctagccat ttcagcttgt taaacaccac ctcgtcttgg
     8521 gcatcaggga ttatcctttt cagcttctcc tccacggtct cggtgggatg gtagggtagg
     8581 aagctattca aaaactcgcg cttggttagc ttttcgctgt tagccatttt ataggtgtcg
     8641 tcggtcatgc caagctgaac aaacacattc catgccttgc tatacccggc acgacgcaga
     8701 gtgcctcgta ttatggtctg tatatcttca atcccatata tctccctgta cttgagtgag
     8761 tcgcggttgg ggtacatctc aaacatgccc aaatccatga tattgtactc ccttacgcta
     8821 gtaaaaagac ggtggtatgg ggtgtacttg tactgtccgt tctcaaggta ctgagcaacc
     8881 ccttgtccag ccagaacaac gttccggggg ttccatgtaa acttgtagtt ccatgggttg
     8941 ttgtcacttt caggcgcaat tagaccgccg gtattgctta tgaatccggt tagctttcca
     9001 cccttggccc ttatctcgtc aataacccgc atggccgaca tgtggtcaat acccgggtca
     9061 accccaagct catttagcag ggcaatccct ttctgccggg cctcctcatg caacgatttc
     9121 atggtgtccg aaacatacga ggcagtaagc atgtgcttgc cttgcctaac gcactcctgg
     9181 gcaaccaccg gatgcataaa tgcaggcagc aacgatatta cagccgttgc ccatgatacc
     9241 tcatcctgca gctgcttgtt attgttgata tcgaattgta tggcttgacc attgggatga
     9301 ttatttacct tttgccgggc ggtttccaac gataaatcgc caactcgtac agtccagccc
     9361 agcactttag agtggtccag caggtacttg attaaatcgg acgatgacag tcctgcccct
     9421 agtacaagaa tcttactcat atctcaaggt ttaggttttg gtttatagct tttggtttaa
     9481 atttggtgcc aaactataat tttttaggct gataaactat gattttgtaa actttttttg
     9541 tttttctgac tacctttggg tagtaaacgt tgagtaccat gattctgctt tttgtaggcc
     9601 tatgggaact ggttgtaatt gttattgttg ctttgctgct ctttgactcg gcaaccctgc
     9661 taaatgttgc ccgattactg ggaagagcct accactacct gcaaaaaagt atcgccgaga
     9721 ttcgcagcca aataagccta atcaacctgg acgacaaaga ccctgaaaac gatgacaacc
     9781 ccagcagtaa gcatgctggt tagattgact ttccacacaa cgctcagtat gacagttgat
     9841 gttgatggct gttggctact cgttgagggc aaatccaatg gaacaccgat tacgcggatt
     9901 gaaaggattt actctgatag ggaatcaaca aaacactaaa cactaaatac taaacacctt
     9961 cccctttccc ctttttttat cctttaacct ttatccttta ccctttaact aaaatgttta
    10021 cctttgttga acttcatttt ttagttttgc tagatgataa aggttgaaag catagttaaa
    10081 tcgtttggag cgcttaccgt gctcaagggc attacggttg agatacctga acggaaagtg
    10141 gttgcaattg ttgggccaag cggggcaggt aaaaccacat tgctccagat tatgggaacg
    10201 ctcagccagc ccgataaagg taaagtgcta tacaatggca ccgatgttac agcgcttaac
    10261 gatacacagc ttgctcgttt ccgcaatagc catattggct ttgtatttca gttccaccac
    10321 cttttgcctg agtttacagc tcttgagaat gtatgcatgc ctgcattaat tgcaggcaaa
    10381 agcatgaccg acgcctcaaa tcgtgcaaag gaactgcttg attttctggg tttgtcacac
    10441 aggtttggcc ataagccatc ggaactatcg ggtggggagc agcagcgtgt ggctgttgca
    10501 agggctctca tcaacaaccc catggtagtg ttagccgacg agccctcggg caacctcgat
    10561 tcacataaca aggaggaatt gcacaggcta ttctttcacc tgcgcgatac ctttgggcaa
    10621 acctttgtgg tggtaaccca cgataaggaa ttggcccgca tggccgacct gcaaatttcc
    10681 atgaacgacg gtcaacttgt ttagccatgc ttacacccag gtcgtttgct gagcttgccg
    10741 atttgcttaa caacaggtac aggcggtatg cctgccctgc atttatcatt ggcgatccca
    10801 ttcaagtacc ccatggcttt tctgataagc acgatattga gataagcgct ttgtttgctg
    10861 caacctttgc atggggcagc cgaaaatcga tagtgaatag cgccagcaag cttatgggca
    10921 tcatgggcaa taagccctac gagtttgtag tgaacttttc caaagccgac gagcatgccc
    10981 tgcaaggctt tgttcaccga accttcagct cagccgatgc cattgagttt gtttacgtac
    11041 ttaggcacat atacacccat ttaggcggac ttgaacaggt atttgccaag ggttaccgcc
    11101 aacagcaatc ggtacacgat gccatacagg cctaccgcaa tgtatttatg gaatgcgagg
    11161 ttcctgcacg cactctccgc catgtcccca atgttgctgc gggtgctgcc gccaagcgta
    11221 taaatatgtt cctgaggtgg atgattcgcc cggcaactga gggggtcgat tttggcatct
    11281 ggagaaccat tccaacctcg gcattactta tgccgctcga tatacatacc ggcagggtag
    11341 cccgtaagct gggaatcctg caacgcgcgc aggacgattt taaagccgtg gttgagctta
    11401 ccgacgcgct aaggcagttt gatcccgccg atcccgttaa gtacgattac gccctttttg
    11461 gtttaggcct tcatgaaaag ttttaacttg caactccgtg ggtaatagct actttaaatt
    11521 caagcaattc acagtgcacc agtcaaaagc ggccatgcgg gtaaataccg atggtgtgct
    11581 tttaggggca tgggctaatg taaccgacac taaccgaata ctcgacattg gcacgggtac
    11641 cggggtaatt gcgcttatgc ttgcccaacg caacacaggt gcacacatcg atgccattga
    11701 gattgatgcg caatcggcag ccgaggccag gagcaacgtg aacctatcgc cctgggctaa
    11761 ccgtatcgat gttatcaact gctcgctgca gagttttgca ccctcggtaa gccaccggta
    11821 cgacctgatt gtttccaatc caccatactt caaccagtcg ctcaaatcgg ctcacaccgg
    11881 ccgaaacatt gcgcgccata ccgagagttt accctacaac catttgattg agggcgtcat
    11941 aaagctgctt gccccaagcg gcaggttctg tggagtattc ccttactccg agggtaatgt
    12001 attcattgcc ctggctggcg cacatggcct ttactgcacg caaaaggtta acgtgcagtc
    12061 gcgacccggc cgaaaggtgc taagggtcct gctccagctc gaaacccaaa aacgggctgt
    12121 ggccgaaagc accctcacca tccacaaccc cgatggttcc tactccgatg agtacaaacg
    12181 gttaaccgag gatttttact tggggtttta gagatttatg atacaagcag ggaatataca
    12241 ggtgctctgc tctcaatttt agcaccatat tctgtactat gcagatacca tgctaacaac
    12301 ttgtaatttt tatgcaaagc cgttctgcca aattttctat ttcgtgatgt tttgcaattt
    12361 gaagtaccca ttcttcggga atattttcaa aaccgtaaag caagcctgca aggcctcccg
    12421 ttacagctcc tgttgtatca gtgtcatatc ccaaattgac cgctttcaaa acggcttctt
    12481 tgaaattact tgttgtcatt aaacaccaaa tactggcttc aagagtatgc aacacataac
    12541 cagtactata aatttgctcc gcgtcaagtt cgtaaatact tgattttagc aatcggtcaa
    12601 acaaagatat ttctgctggg ttaattgaaa gatttgttaa atgatttaaa actaatgttt
    12661 gcaaattgct gtaaatatca aacttgtctc ttccctcaat aatttgcata gcgaattcaa
    12721 ggtagtaaaa gcatgcaata attgaacgga tatgcctgtg agttattgaa gatacctttc
    12781 ttgtaatttc gaaacgcaca ttaattggct tgttatataa ataaaaaagc agcggtaaga
    12841 ttcgcattaa cgaaccgtta ccgttatcgg tttcttttga actacctgcc aactcaggat
    12901 tctcgccatt tgcaattcgt aaaattgcct gtttggttgt tccaccaatg tcaaaaactt
    12961 cgctattggg tgtccaataa ttttcgttgt accattttac aaaattctgt cctatttcat
    13021 caaggcaaaa gtcattagtc aatccttcgg ccaaacaaaa agttagcgaa ctgtcatcgg
    13081 accaagtccc aggaggctga ttgtgtgtgc cgtaaccaat catagtagtt acaggatttt
    13141 ggcttaattc ttgtctgctt ttaaactcta ctggaacccc taaagcatcg ccaactgcaa
    13201 ccccaaaaag tgcagattta atttgtttaa gagtcctatt atctttacga atttcgtttt
    13261 gcattaaccc ttttttaaac tttaaagtaa aaaatccatt aaaaaactat ttgctcatct
    13321 ttggcggggc ttatagtcaa tgaaaaatac ttttcataca ttttatcgct tgcttgaaat
    13381 tccatattat gctttttgca atagtagtgt ggaaaagggt ttagcacgga gtgtcatagt
    13441 gcattttttt atcacgttgt caaaatgttc catgttatac aattaagtat tccgccattg
    13501 ttagccactt cgttactgtc aatagttgct atttgttgtc ctctaaaaag ttgttcaaat
    13561 tgtcgaactg cgatatcatc ctccttaatg ccaaaagttg gaacgataac cgtattttgc
    13621 atttgtaaaa agttgatgta gataccattt gcttgtccgt tagtcttgtt gccgtatggg
    13681 ttgtatggga tttcaatgta gtcaagacct gtgttgtcca atgcaatttt aaatgctctc
    13741 tgaaattctg gtttctcttt tgaatagtcg ttaattatta ccgtattgtt gtcaaggaaa
    13801 cgaaccattc catctgcgtg tccagtaaaa tcctttggtt gttgagggac aaaaaagagt
    13861 ttatctattt caaataattc ttttaattta tctgaaagtt gtcgccttga atagtttgga
    13921 ttttcaagaa aaatcttgtc gcacattatt actttatcgg ttgtctttat tacgttacct
    13981 ccatctaaaa cgatgtctga ttttattcta ttaagtctta ttgagttgca aatactttca
    14041 acatccgtaa ttgtctttat ccatttccgg ctttgcaaat agtctgggtt gtaaacaaac
    14101 tgaacaaact tgtcaactgt aacttgaact ggcatataat caaccgccca aatatctttc
    14161 gtgtcaggta atagctgaaa agtgatacca cactttttta atatcgcttc aaagtccata
    14221 aagaattttg gatgcttttt tggtaagcag tcagcaatgt aaagagtatt tgtttggcta
    14281 tcgggtatca tttacggatt tatgtcgtag cccattattt ctttggcttg ttttttattg
    14341 attttattgt ctgccaaaag ttgtcgaaca acttttgctt tatccttgaa tttatgttga
    14401 agatttagtt caactatata ccgatatttt ttctcggcat aattttctgt gtcctctttt
    14461 ttcttagtca agccgatacg aaccgcttgg attggttcgg tttttatctc ttgaattttt
    14521 tctttattta gttctctcaa acaaatgctt cctttgcctg tcaaaatgtt accgatgttt
    14581 gagaaaattt ctttaagaac atttgatggg ccgcttatgt aacgtggact atctccaaat
    14641 ctcataattc ccctgattaa aagcccacca taaattttct tttcatagtc gccaaatgta
    14701 atatctaaac ctgctccgtt aaaataccaa ttgcctgcaa aaagttgttc tgtagcacaa
    14761 tgaacgtaag gatccgggtg attgtcttta tcgtagtagt aaacttccaa ctctgtaatt
    14821 ctgtatggtt ggtcggcaat aactaaacac aaataattca ttaaatagta ggccaatgtt
    14881 gaaaacttct cgtcaagcga tttgatttca gggcacccgt ttaagtaatg ccaaaattct
    14941 gcaagtttac tttgttcaac ttcttcgtct ttactctcaa gttcaaatga tgaaaggtaa
    15001 aggtcgaggt atttaatgtc tttcacatcc gttcaaaata aaattttgta gctaaaaaat
    15061 ggaagttgga aatatccctg taaagggata gttttacatt aacccaaaaa ccaactccca
    15121 tggtaaagtt aacacttttt ccccaaatca ccagccttat ttcccagcat atattcttat
    15181 gttgagtcga ggctgtaaag agcaataaac gccccgtgtt cggcggcggt tgcaccgccg
    15241 tcgggctatc agtagcaggc tgtgcc
//
LOCUS       DAOOWJ010000062        15043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3986_length_15043_cov_7.563050, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000062 DAOOWJ010000000
VERSION     DAOOWJ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15043
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_15043_cov_7.563050"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLW22_07340"
     CDS             <1..709
                     /locus_tag="PLW22_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU74555.1"
                     /translation="SPVIAVNHPASPGNIRIGSVGPVVPNVMVKIADDGEILMQGSSL
                     MKGYYKAPDLTQMAIDEEGWFHTGDIGHLDDGKFLTITDRKKEIFKLSNGKYVAPQLI
                     ENKLKESFFIEQCMIVGENEKFVSALISPNFSFLHDWCSRHKIHFENNEELITIPEVI
                     ARYQKEITTVNKQLSDYEQIKRYRLVAEQWTPQTGELSPTLKLKRKVLYERYEKILEE
                     IYGHAKQTEVRGVNKEL"
     gene            complement(773..1447)
                     /locus_tag="PLW22_07345"
     CDS             complement(773..1447)
                     /locus_tag="PLW22_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74556.1"
                     /translation="MDEFMKTLSFIIGLIFVSVSAKGQELYQKSWLIDVGLGSPMLKK
                     GETNPVQARISVGYEIQFTESGLLGLAMRGGYAITASHAYNINETGLPSTDHELLYSY
                     TGNSFFAAVSPMLIFEQYDSNVDRIFAELELGLASHGVNIKFYEPNPVTSSNWVYFKG
                     FFALKIGLRVAWEKNSKSRAMSIWGALHNQSPIDAIDKSIPVEWPYKFNEDLNSQWQV
                     GISVYL"
     gene            complement(1489..2022)
                     /locus_tag="PLW22_07350"
     CDS             complement(1489..2022)
                     /locus_tag="PLW22_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74557.1"
                     /translation="MKFKCFYFGLFIIPSLTLNAQESMFLQKNHSVSMEVLGLTYNYE
                     SPIAKRVTLIGRLGMQGGAGYSSSLGSYYAISPAISFETRFYYNILKRESSGKNIKGN
                     SANFLSLSTWYISNPVIEHKSSGADFFAVTPQWGVRRVYWKHFMLEAGAGISYFFNLE
                     GDSKTNLMLNLRLGYMF"
     gene            complement(2465..3928)
                     /locus_tag="PLW22_07355"
     CDS             complement(2465..3928)
                     /locus_tag="PLW22_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU74558.1"
                     /translation="MSTSLIIAVIIGYFVTLFVISYFTSRNSNNDTFFIGNRKSPWYL
                     VSIAMIGTSISGVTFISVPGWVLTSQFSYMQMVLGYLLGYFVVANVLLPLYYRLNLKS
                     IYTYLDARFGFYSYKTGAFLFLVSRVIGSAFRLFIVSNVLQLSIFNALGVPFWVTVSI
                     TVLLIWLYTRKGGVRTVLWTDSFQAIAMLTAVSLCVYYISSDLNLSLPGIVKAVSSSP
                     LSRVWFFDDPNQRFYFWKQFLGGAFITIVMTGLDQDMMQKNLSCRNYKDARKNMYWYG
                     FAFLPVNLLFLSLGVLLHTFAVQKGIPLPALSDDLFPTLATQGYLPQIVGVLFFLGLI
                     AAAYSSADSALTALTTSFTIDVLNTKGMDEDRLRRVRQWVHVFISILMILVIIVFNAF
                     NDRSVIDAIFTIAGYTYGPLLGLYAFGLFTRFQVNDKLVPFIAVFSPLACYLLNKYSI
                     LLFNGYKFGFELLIVNGAIVFLLLLITRKRGVLVQKV"
     gene            4082..4711
                     /gene="recR"
                     /locus_tag="PLW22_07360"
     CDS             4082..4711
                     /gene="recR"
                     /locus_tag="PLW22_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOU74559.1"
                     /translation="MQTHTYPSKLLERAVEELAKLPGIGNRTALRLALHLLRQPQADV
                     EQFLTAIGEMKTNIHFCSVCHNISDTDICSICSSPSRDKTTICVVETIKDVMSIENTM
                     QYKGVYHVLGGVINPMEGIGPNDLTINSLVERLSTSEVNEVIMALSATTEGDTTNFFI
                     YKKIKNFPVMVSTLARGVAFGNELEYTDEVTLGRALLNRVRFDNSFSVE"
     gene            4768..5496
                     /locus_tag="PLW22_07365"
     CDS             4768..5496
                     /locus_tag="PLW22_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74560.1"
                     /translation="MFKYCLTLIIAATISLFAVAQSNISDLIANGNQKIKEKDFKGAL
                     AEFKNALNVSPNDSLALNGIIRANTLLEDYKEAQRWIAIALEAYPNNPDFLFRRGIIQ
                     NLNGYHDKAIEDFESALGQNPNTSLKVQILLNKASAHFKIEQFAESLADYNKVVELDP
                     RNFNAYNFRGLVNFRLGYYIDAVTDYTTAIDLDPTSPLPYYNRGMSLLKLNENQKACA
                     DFRKACQMGYMTSCKMVVAECGAR"
     gene            5945..7156
                     /locus_tag="PLW22_07370"
     CDS             5945..7156
                     /locus_tag="PLW22_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOU74561.1"
                     /translation="MINDLDQILSASAKRMKRSVIRELLKMTQRPELISFAGGLPSPE
                     SFPVEQLKQVVVEVLDTDSARALQYSETEGDKRLREILVEKYRKEGLNININNLIIST
                     ASQQALDLIPKIFVNPGDKVICGLPSYLGGISAFATYGADLVGIELDDHGMRSDLLEQ
                     KMQELQRKGQKPKFIYVIPDFQNPTGICMPERRRLEIIEIARKYNVLIVEDSPYREVR
                     FSGTPQRTMYELDGTGQVILLGTMSKIFVPGFRLGWIVAHEEVIDKLVVAKQNTDLCT
                     SSFVQKIAAKYFDKGYFEPNLAKTNAMYKEKRDAMVEAFHKFMPKGVKWTEPEGGLFL
                     FVTLPEYIDAEDLFKIAIEENVAFVPGTVFYCNGEGKNTLRINFSFMSKELNIEGSRR
                     LANAIKRMIKE"
     gene            7392..9326
                     /locus_tag="PLW22_07375"
     CDS             7392..9326
                     /locus_tag="PLW22_07375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU74562.1"
                     /translation="MELSVVVVNYNVRYFLEQCLVSVFAALKGIEGEVWVVDNASTDG
                     SVEMVKLKFPQVKLISNQTNLGFSKANNQAIKLAKGRYTLLLNPDTVVQEDTFRECIN
                     FMDAHPDAGGLTVKMIDGKGRYLPESKRGFPSPWASFCKIFGLTSLFPKSKLFAHYYL
                     GHLDKNATHAIEIMPGAFMFLRSEVLNQVGLLDEQFFMYGEDIDLSYRILQHGYKNYY
                     YPKCQIIHYKGESTKKGSLNYVIVFYKAMILFAQKHFSKQKQNHFVTLIKLAVAIRAT
                     ASIIKRLIIKVWLPFTDFALMFLGAYLLIPWWEQFRHGVTNIYPRDILYTLIGFYLLT
                     WIMSLWLYGAYDKPQNLKSGAKGILYGTIAILVVYSILPQNFRFSRAIILILSAWSLL
                     SITLNRILASTFIEGLLKWKARKKSVCFIGTASELKGSEELLIQAGFHRNNITHLLPE
                     EVLDEHQRAKKEIIADAIKYKNISEIIFGTEGIPMTSIIHAMMYFSEFDIDFKIALHG
                     GDSIVGSSSVEAQGELYTLEVIPLAKPVARRQKRIFDILSSALIIVLFPILWIFLVKP
                     FMVLKSALQVFKGEKTWIGYNHPNISKQNSVLKPSVYLYSKWGKGLTSNVGLDFYYAQ
                     HFSITLEVSELIRNIFKTQD"
     gene            9432..>10705
                     /locus_tag="PLW22_07380"
     CDS             9432..>10705
                     /locus_tag="PLW22_07380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOU74563.1"
                     /translation="MAEVKIVLPAMGEGVTEAKITKWLVKPGDLVEVDQPLVEIATDK
                     VDSEVPSTSKGKVKQLLFNEGDSPQVGQAICILEVEGVIAEGSKTHVAPAEPKSAQKE
                     KPIAKPIEITEKAEVKQEIKTLRYLSPLVRSMAKQENIALEELENIEGTGLDGRITKD
                     DLLKYIENRSNRISQIEAEAKERLQTETPIQYTSSTDTNISVLAHEVVPMDRMRKLIA
                     EHMVRSKQTSAHVASFIEVDVTNLVNWRETNKDKFQKTYGDKITLTHLFAQATIAEVK
                     RYPLLNSSVEGDNIILKRDVNLGIATALPNGNLIVPVVKRAETLNLIGIAKSINDLAQ
                     RARDNKLKPDEIVGGTFTITNLGSFDTLTGTPIINQPQVAILAIGAVKKRPVVIETPS
                     GDMIAIRHICILSLSYDHRVIDGALAGQFLKS"
     assembly_gap    10706..10805
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10806..12059
                     /locus_tag="PLW22_07385"
     CDS             <10806..12059
                     /locus_tag="PLW22_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74564.1"
                     /translation="ISFSSSSAQRSSYYRRIFVDAEYYMLYEEYRDALPLYQELYKAY
                     PNNYNLAYRIGLCYLNIPNEKAKSLPYFEKAITSISDSYKEGYFTETNAPKDAYLHYG
                     RALRILGQFDKAQKAFETYKNLLKPNEITELRIVRKELEALDNARYFTSNPLKHRMTP
                     VGRNITTRFPEVNPVSDSSGNTLVYTSVQRFYNAILISNRGNEFWNNPLNLNPQLYAD
                     GDIRTVGITSNGNVLLLSRNDNDIYNLYYSTYNKETNSWAPITKFPKEINTSKSWQTF
                     GSLSRNGDTLYFSSNREGGFGGFDIWMSIKTSNGWSSPINLGKNVNTPFDEIAPFLTE
                     DGKRLFFSSNGLKTMGGFDIFYSDYENKAWGIPINVGYPLNTTDDDTFFFPIKNGKEG
                     YYSKNSESKGDEDIYHVALDIKSPR"
     gene            12063..14411
                     /locus_tag="PLW22_07390"
     CDS             12063..14411
                     /locus_tag="PLW22_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74565.1"
                     /translation="MKAFRISVFLLLIAWLPFYSSGQTKTEIDETYRDANSYFYFEDY
                     EEALALYQKIYKFQPDNYNLNYRIGFCYLNIPGSKHKSIQFLEKAVQNTTTRYNEESI
                     TEQRAPIDAIFYLGNAYLTVNRIDDALKAYEKFYSLTKGKSDWDFDYLNHQINVAKNS
                     LKIQRNPVDFIMANLGEKINDRFPNFNAVVSGNGKVLAFTTKLKFYNAINISRKNEKN
                     QWEKPVNITLDLQTDISCSTLCLSYDGNELYLFRDDNHDGNIYVTRFNGKKWLPIKKL
                     NPNINTEAYETHASLSPDGKQLFFTSNRKGGFGELDLWVSTRTSGDDWGPARNLGATI
                     NSPFNENTPFISTDGTTLYFSSEGHENMGGYDIFVSQLSGKGDWTTPINLGYPLNTTD
                     DDLFYHPIEDGATALIARFDDNSFGETDIFEIELFIPRFRKSIVPKTVAQERTTDKTF
                     ARLIVDTLSTKGMASIDANEAITKIDISGNNTLKLFLNGKAYGVIPSRIEELAEAKRV
                     SETENAAKAYTEETDNQTPSPISSNSATDATFKNNFGDTTPRSQWPTDTPTVKQKAFE
                     LNNASINDDSYFSEILLLLTPNEKQQDILPVLKRNWKFSTENLNETIIHFASSFKTDK
                     ELNTIIYSLTELYDKIGNLSNTQSGKKEQKFAKNNRTLIIKAYNKLINKASKQLGNDL
                     ALVMLKNKEIGNLTQLIELYSKQNPEGFNANREELTLLLARMAISEYVALPNEQKISL
                     YNSSTGKTSVEPSYIWLWVLLTLIAMGVIIYLGIEYYKKKTS"
     gene            complement(14408..>15043)
                     /locus_tag="PLW22_07395"
     CDS             complement(14408..>15043)
                     /locus_tag="PLW22_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOU74566.1"
                     /translation="LRPGLAFFLQQISIDFSKLIFSDQLTSTPMPPTSVTQPGKDAVW
                     DIDVSSSLLAYSDNIWLGVTWDHMLKPVATFYDDNSRLPYKITVHGGYRYITKGFLLS
                     RIEESFTVAFNYRVQDIYRQLDLGLYFYSQPISFGVWWRGLPKNSVGRRVDALAFMVG
                     YKWEQVSMGYSYDFTISRLGLASGGSHEISMIYEFRLTPKKRWKAIPCPTF"
BASE COUNT         4914 a         3144 c         2944 g         3941 t
ORIGIN      
        1 ctcgcctgta attgcagtta accaccctgc ttcacccggc aacattagga taggttcagt
       61 aggccctgta gtgcctaatg taatggtaaa aatagccgac gacggcgaaa tattaatgca
      121 gggctccagc ctgatgaaag gctactataa ggcccccgat ttaacccaaa tggctattga
      181 cgaggagggc tggttccaca ccggcgatat aggccatttg gacgatggta agttcctaac
      241 aataaccgat aggaaaaagg aaatctttaa actctcaaac gggaagtacg ttgcaccaca
      301 actaattgag aataaactga aagaatcgtt cttcattgag caatgtatga tagtagggga
      361 aaacgaaaag tttgtaagtg cattaatttc gccaaacttc agcttcctgc acgactggtg
      421 cagccgacat aaaatacatt ttgaaaacaa cgaggaactg attaccattc ctgaggttat
      481 tgcaaggtac caaaaggaaa ttactaccgt taataaacag ctcagcgatt atgaacaaat
      541 taaacgttac cgcttagtag cagagcaatg gacaccacaa accggcgagc tatctccaac
      601 ccttaagcta aaacgtaagg ttctgtacga acgttacgaa aaaatacttg aggaaattta
      661 tggccacgcc aaacaaaccg aggtaagagg tgtgaacaag gaattataat gaagtatatt
      721 ttatgaaata aaagaggctg ttaagaacag cctcttttgc ttttaattaa tactacaaat
      781 aaaccgaaat ccctacttgc cactgtgagt tcaaatcctc attaaactta tatggccatt
      841 cgacaggaat tgacttgtca attgcatcta taggactctg attgtgcaag gctccccata
      901 ttgacatagc tctactttta ctgttttttt cccaagctac tcttaatcca attttaagtg
      961 caaaaaaacc tttaaaataa acccagttag aactggtaac tggattaggt tcgtaaaatt
     1021 taatatttac accatggctt gccaacccca actctaattc agcaaatatc ctgtccacgt
     1081 tgctatcata ctgttcaaat attagcatcg gagacaccgc tgcaaaaaaa ctatttccgg
     1141 tataactata aagtaattca tggtcagtac tgggcaaacc tgtttcattg atattatatg
     1201 catgacttgc agttatggca taacccccac gcatggcaag acccaataat ccagattcag
     1261 tgaactgaat ttcgtatcca actgatattc ttgcttgaac agggtttgtt tctccttttt
     1321 ttaacattgg agatcctaag cctacatcaa tcagccatga tttttgataa agttcttgcc
     1381 ctttggcact tacactaaca aaaattagcc ctataatgaa tgaaagtgtt ttcatgaatt
     1441 catccatatc aaaatgttag tatattatat gaactaatac tggaaacatt aaaacatata
     1501 acctaatctc aagtttaaca ttaaatttgt tttgctatcg ccttctaagt tgaaaaaata
     1561 gctaatcccc gcccctgcct ctagcataaa gtgtttccaa taaactcttc ttactcccca
     1621 ctgtggggta actgcaaaaa aatcggcacc tgaagattta tgttctatta ccggattaga
     1681 aatgtaccat gtgctaagcg ataaaaaatt agcagagttt cctttaatat tcttaccact
     1741 actttccctc tttaaaatat tgtagtagaa acgtgtttca aaactaattg cggggcttat
     1801 tgcatagtat gaacccaatg agctgctgta acccgcacct ccttgcatcc ccaatctacc
     1861 aatcaaagta acccttttag caatagggct ctcgtaatta taggttaaac caagcacctc
     1921 catgctaacg gaatgattct tctgtaaaaa catggattct tgagcattta gggttaatga
     1981 ggggataata aataacccga aataaaaaca tttaaatttc ataataaaaa aagatttagt
     2041 aaattgatta ttaaacaatt attaaaacaa gatttaatta ttttgaaaga tatttattta
     2101 tacaattcca aaatatttta aaacaataag aattactcga attaaaaact cacaaaaata
     2161 tatcgtttgt taatatatgc aattacttca ttcgtcatcc tgagcgtagc gaaggatctc
     2221 tcaacgacac tatgagatgt ttcgcttgcg ctcaacatga caatagtttc acgaacaatt
     2281 ccatttgtca tcccgaggac caggagggat ctcatcaaag gggataaaag aaagaggaca
     2341 aagagatgct tcggcgaaca cagagtacag atagatatga cgcaggattc aatcctgata
     2401 tttcgggact caacatgaca agcatccgta actaagcgaa attaaaacac cttattttta
     2461 aactctaaac cttttgtaca agaactccgc gtttacgggt aatgagcaat aggagaaaaa
     2521 caattgcccc attcacaatt aaaagttcaa acccaaattt gtatccatta aacaaaagga
     2581 tagagtactt attcaggaga tagcaagcca atggggagaa aaccgcaata aatggtacaa
     2641 gcttatcgtt cacctgaaaa cgagtaaaca gaccaaacgc gtacagccca agtagaggac
     2701 cataggtata tccggctata gtaaaaatgg catcaatcac tgagcggtcg ttgaatgcat
     2761 taaaaacaat gataacaagt atcatcagta ttgatataaa aacatgcacc cactgcctaa
     2821 cccttcgtaa tctatcctcg tccatacctt tggtattaag gacatcaatg gtaaacgatg
     2881 tggttaaagc ggttaaggct gagtcggcgc tggagtatgc ggcagcaatt aaccctagga
     2941 agaacaaaac accaactatt tgtggcaggt atccctgagt ggccagggta gggaacaaat
     3001 cgtcggacaa tgcaggaaga ggtatgcctt tctgtacagc gaaagtgtgc aaaagcacac
     3061 caagcgatag aaaaagtagg tttaccggta gaaatgcaaa accataccag tacatgtttt
     3121 tgcgggcatc tttgtagttc cggcagctaa ggtttttctg catcatgtcc tggtcaagcc
     3181 cagtcattac aatggttata aatgcgccac caagaaactg tttccagaag tagaagcgct
     3241 ggttcggatc gtcgaaaaac cagaccctgg aaagcggact gctgcttacc gcctttacaa
     3301 tgcctgggag gctgagattt aaatcgcttg aaatatagta aacacaaagc gaaacggctg
     3361 ttaacattgc aatggcttga aatgaatcgg tccatagcac cgtgcgcaca ccgcctttgc
     3421 gcgtgtagag ccaaatcagt aaaaccgtta tggacactgt aacccagaaa ggaacgccca
     3481 aagcattaaa gattgagagc tgcaatacat tcgatacgat gaaaagcctg aatgcagacc
     3541 caataactct tgaaaccaag aagaggaatg ccccggtttt gtaggagtaa aaaccaaagc
     3601 gtgcatccaa ataggtgtat atcgatttta ggtttaaccg gtagtagagg ggtaagagca
     3661 cgtttgccac aacaaaatac cccagcaggt agcccaacac catttgcatg tatgaaaatt
     3721 ggctggttaa aacccatcct ggaaccgaaa tgaaggtaac accagatatg gaggttccta
     3781 tcatggcaat ggataccaaa taccagggtg atttacggtt accaataaaa aaagtatcgt
     3841 tattggaatt gcgcgaagtg aaataggaaa tcacaaagag agtaacgaaa tatccaatga
     3901 ttacagcaat aatcaggctt gttgacatac ggcaataaat ttgagccaac aagtgtatga
     3961 aaaaatctac acattacaaa aactaagcaa ggctgcaaac ctatttgagc gaaaatcgtt
     4021 taatgtatca tcgcaataat ctactcttat taactagatt tgccaaacaa acgacattaa
     4081 catgcaaacc catacatacc catcaaaact gctggagaga gctgttgagg aattggctaa
     4141 actccccggg atagggaata gaactgcgct aaggttagcc cttcatttac ttaggcagcc
     4201 ccaagccgat gttgagcaat tcctaactgc tattggcgaa atgaaaacaa atatccattt
     4261 ctgctccgtt tgtcacaata tttccgatac cgatatctgt tcaatatgtt catcgccatc
     4321 gcgcgataaa acaaccattt gtgtggttga aaccataaag gatgtgatga gcattgaaaa
     4381 taccatgcag tacaaaggtg tttaccacgt gctgggtgga gtaataaacc ccatggaggg
     4441 aattggccca aacgatttaa ccattaacag cttggttgaa aggctgtcga ccagcgaggt
     4501 aaatgaggtg ataatggctt taagcgccac aaccgaaggc gataccacca acttcttcat
     4561 ctacaaaaag attaaaaatt tcccggtaat ggtttccacc cttgcccgag gtgtggcttt
     4621 tggcaatgag cttgagtata ccgacgaggt cacgcttggc cgtgccctgc ttaaccgggt
     4681 aaggtttgac aattctttct ccgtggaata atttttgaac tttaacaaaa agattgtatt
     4741 tttaaaacat cattattcta tacaaacatg ttcaagtatt gcttaaccct tataatagct
     4801 gcaacaattt cgctttttgc tgttgcccaa tccaacatta gcgatcttat tgccaacggc
     4861 aatcaaaaaa ttaaggaaaa ggatttcaag ggcgcccttg ctgaatttaa gaatgcgctc
     4921 aacgtatcgc ctaacgactc cctagcttta aatggtatta tcagggcaaa tactctgctc
     4981 gaggattaca aggaggctca acgatggatt gccattgcgc ttgaggcata ccctaacaat
     5041 cccgatttcc tgtttcgtag gggtattatt cagaacttaa acggttacca cgataaagct
     5101 attgaggact ttgaaagcgc cctcggccaa aaccctaaca cgtcgctcaa ggttcaaatc
     5161 ctgcttaata aggcttcggc ccattttaag attgaacagt ttgcagaatc gcttgccgac
     5221 tacaacaaag tagtggaact ggatccgcgc aattttaatg cctacaactt ccgagggctt
     5281 gttaacttca ggcttggcta ctatatcgat gccgttaccg attacacaac cgctatcgac
     5341 ctcgatccaa catctccact accctactac aacaggggca tgagcctgct taagctgaat
     5401 gaaaaccaaa aagcctgcgc cgattttcgt aaggcttgcc aaatgggtta catgacctcc
     5461 tgcaagatgg tggtggcgga atgcggagcc cggtagaaaa gttaataaat atattgctca
     5521 ataaaaatta gtccctattg gggacttttt tatttaactc aaacatcatg aactaaacaa
     5581 ggctaatttt agaggaatga accggaatga gtcggaataa acagaatatg atggagttag
     5641 aatttgaaga agttagaaga agttagagga agttagagga atagtgaaaa gtgaaaagca
     5701 aaagcgaagc atcatcaatt aacaaattca actattaata taatgaacgc agtaacgttt
     5761 ttagagtcgg gattacatct ataagccaat agaaacaagt tatttaattt tttgtatgta
     5821 aactgaacaa tctcggtcta aaaattttga aatatctaat tacatttttt aacttagcag
     5881 caatatttag taatagatat tttgtaatag ccttggttta ataacatatt ataagaaaaa
     5941 caccatgatt aacgacctag atcaaattct atcggcaagc gctaaaagga tgaagcgttc
     6001 agtgataagg gaattgctaa aaatgactca acgtcctgaa cttatctctt ttgctggagg
     6061 tcttccttcg cctgagagtt ttccggttga acagctaaaa caggtagtgg tagaagtatt
     6121 agataccgat agcgctcgcg cccttcagta tagcgaaacc gaaggtgata aaaggcttcg
     6181 ggagattctg gttgaaaagt ataggaaaga agggctgaac atcaatataa acaacctgat
     6241 aatctccacc gcatcgcaac aggcgcttga ccttattcca aaaatatttg ttaaccctgg
     6301 cgacaaggtt atttgtgggc tacccagcta tttagggggg atttcggcat ttgccacata
     6361 cggagccgat cttgtaggca ttgagctcga cgaccatgga atgcgctccg atttgcttga
     6421 acagaaaatg caggaattgc aacggaaagg tcaaaagccc aaatttattt acgttatacc
     6481 tgacttccag aacccaaccg gaatctgcat gcctgaaagg cgaagactag aaataattga
     6541 gattgctaga aagtacaacg ttttaatagt tgaggacagc ccataccgtg aggtgcggtt
     6601 cagcggaact ccacagcgca caatgtatga gctggatggt actggacagg ttatattact
     6661 tggcacaatg tcgaaaatat ttgtacccgg attccgcctg ggctggatag tagctcatga
     6721 agaagttatt gacaagttgg ttgtagcaaa acaaaacacc gacctttgca cctcgtcgtt
     6781 tgttcaaaaa atagccgcta aatactttga caagggatac tttgaaccca atttggctaa
     6841 aaccaatgcc atgtacaagg aaaagcgcga tgccatggtg gaggcctttc ataagtttat
     6901 gcccaaaggt gtgaaatgga ctgaacccga gggaggactc ttcctatttg ttaccctgcc
     6961 tgaatatatt gatgccgaag acctattcaa aattgcaata gaggaaaatg tagcctttgt
     7021 tccaggcact gtattttact gcaatggtga gggcaagaat actcttcgaa tcaacttctc
     7081 attcatgtcg aaagagctaa atattgaagg aagccgaagg cttgccaatg caataaaacg
     7141 aatgataaaa gagtaatact tatcatctac acatcttgta gccactgagt ttaaatttgt
     7201 aaaagattat tcagtggctt tttttttgga ttttacattg gaattaaacc tcatttacct
     7261 tgccctgttg gtacgctttt ttatccgaaa tccttctcat cagcaagttt taagtattac
     7321 tgctaggaat acctgcattt ttgcaacatt tttgtatatt tccacaaaat taatcggcgt
     7381 tttactgatt catggaactc tcggttgtag ttgtaaacta taatgttcgg tattttttag
     7441 agcaatgcct tgtaagtgta tttgcagcac ttaaagggat tgaaggcgaa gtatgggtag
     7501 ttgacaatgc ttcaaccgat ggttcggttg aaatggtaaa acttaaattt ccccaggtaa
     7561 aactaatatc aaaccaaacc aaccttggct tttccaaagc caacaaccag gccataaaac
     7621 ttgcaaaggg aagatatacc cttttactaa accccgatac ggttgttcag gaggacacct
     7681 tcagggagtg cataaatttt atggatgccc atcctgacgc cggcgggtta acagttaaga
     7741 tgattgatgg gaaggggcgc tacctacccg aatccaagcg agggttccct tcgccttggg
     7801 catcgttctg caaaattttt gggctaacct cacttttccc taaatcaaaa ctttttgcac
     7861 actactacct tggccatttg gataaaaatg cgacccatgc aatagaaata atgcctggcg
     7921 ccttcatgtt ccttcgttcg gaagttctaa atcaggttgg actgctcgat gagcagttct
     7981 ttatgtatgg agaggatatc gacctttcat accgtatcct acaacacgga tataagaact
     8041 attactaccc aaaatgtcaa ataatacatt acaagggcga aagcacaaaa aagggtagtt
     8101 tgaattacgt aatagtattc tataaagcca tgatactgtt tgcacagaag catttcagca
     8161 aacagaagca aaatcacttt gtgactttga taaagctagc cgtcgcaata cgggcaacgg
     8221 cctcaataat aaaaaggtta ataattaaag tttggttacc atttaccgat tttgccttaa
     8281 tgtttttggg agcctaccta cttatacctt ggtgggagca atttaggcat ggtgtaacca
     8341 atatataccc ccgcgatatt ctttatacgc taatcggttt ctacctttta acctggatta
     8401 tgtccctttg gctgtatgga gcatacgata aacctcaaaa cttaaaatcc ggagcgaaag
     8461 gtatactata tggcacaatt gccatactgg ttgtttactc cattctacct cagaacttcc
     8521 gattttcacg tgccatcatt ctaatcctat cggcatggtc attactatca atcaccctaa
     8581 acagaatatt agcatcaaca ttcattgagg gtttacttaa atggaaagcc cgaaaaaaaa
     8641 gcgtttgctt tattggcaca gctagtgagc tcaagggttc agaagaactt ttaatacagg
     8701 ctggttttca ccgtaataat atcacccacc tgctccctga ggaggtttta gatgagcatc
     8761 aaagagcaaa aaaagagatt atagccgatg caattaaata taaaaatatc tccgagataa
     8821 tttttggaac agagggcatc cctatgacaa gtattattca cgccatgatg tacttttcag
     8881 aatttgatat cgattttaag attgctctac atggtggcga ctcaattgtt ggcagcagtt
     8941 ccgtggaagc ccaaggtgag ctttacactc ttgaagtaat accactggca aaaccagtag
     9001 caaggcgcca gaaaaggatt ttcgacattt tatcgtcggc tctgataatc gtgcttttcc
     9061 ctattctatg gatattcctt gtaaaaccat ttatggtttt aaaaagtgca ctgcaggttt
     9121 ttaaagggga aaaaacatgg attggctata accatcctaa catttccaaa caaaattcgg
     9181 ttttaaaacc atcggtttat ttgtatagta aatggggcaa aggtttaact agtaatgtgg
     9241 gcctcgattt ctactacgct caacatttca gtatcactct ggaagtatcg gagctaatca
     9301 ggaatatttt taaaacccaa gattagcatt aaaaaccatt ttgtctggat tttcttcaat
     9361 tttggtctga tgactttcaa ccttttacac aattaggtgt taatagaggt gaaaaagaaa
     9421 attcaaataa catggcagaa gtaaagatag tactcccagc aatgggcgaa ggtgttaccg
     9481 aagcaaaaat tacaaaatgg cttgttaaac ccggcgattt ggttgaagtt gaccaacctc
     9541 tagtagaaat agcaaccgac aaggttgatt ccgaagttcc atcgaccagc aaaggtaagg
     9601 taaaacaact actttttaac gaaggcgact cgcctcaggt tgggcaagct atttgcatac
     9661 ttgaagtgga aggggttatt gcagaaggaa gcaaaacaca tgttgctccg gctgagccta
     9721 aatcggcaca aaaggaaaaa cctattgcta agcctattga gattacagaa aaagcagaag
     9781 ttaaacaaga aataaaaacg ttgcgttacc tttcaccgtt ggtgcgtagc atggctaaac
     9841 aggaaaacat tgcccttgag gagttggaaa atatagaagg taccgggtta gatggccgaa
     9901 taaccaagga cgacctcctg aagtacatag aaaaccgtag taatcgaatt tctcaaattg
     9961 aagccgaggc caaggagcgg ctccagaccg aaacacccat tcagtacact tcgtccaccg
    10021 atactaacat tagcgttctt gcgcatgaag tagtaccaat ggatcggatg cgcaaactaa
    10081 ttgctgagca catggttcga tccaagcaaa cctcggcaca tgtagcctcg tttattgagg
    10141 ttgatgttac caatttggtt aactggcgcg aaaccaataa ggataagttc caaaaaacat
    10201 acggcgataa gattacacta acccacttgt ttgcacaggc aaccattgct gaagtaaaac
    10261 gttacccact gcttaactcg tcggttgagg gagataatat cattctaaag cgcgacgtta
    10321 atcttggcat tgctactgct ctacccaatg gcaatctgat agttccggta gtaaagcgag
    10381 cagagactct caacctgatt gggattgcaa agagcataaa cgatcttgcc cagcgggcac
    10441 gcgacaacaa actcaaaccc gatgagattg taggagggac ttttaccatt accaaccttg
    10501 gctcgttcga caccctaaca ggaaccccaa ttattaacca accccaggtt gccatcctgg
    10561 caattggtgc tgtaaagaaa cgtcccgttg ttattgaaac cccatcgggc gatatgattg
    10621 ccatacgtca catttgcatc ctttcactat catacgatca tcgagtgatt gatggagccc
    10681 tcgcagggca attccttaaa tcgttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnatttc gtttagtagt tcgagcgctc aacggagctc ttactaccgt cgtatatttg
    10861 ttgatgcgga atactacatg ctttacgagg agtaccgcga tgccctaccg ctataccagg
    10921 aactttacaa ggcctatccc aataactaca acctagcgta ccgcattggc ctgtgttacc
    10981 tgaatattcc gaacgaaaaa gccaaatcgt taccctactt tgaaaaggca ataacttcga
    11041 tttcagacag ctacaaagag gggtatttta ctgaaaccaa tgcgccaaaa gatgcttacc
    11101 tccactatgg ccgggcactg agaatattgg ggcagttcga caaggcgcaa aaagcctttg
    11161 aaacctataa gaacctactt aaaccaaacg aaataactga acttagaatt gtaagaaaag
    11221 agcttgaggc acttgataat gcacgttact ttacctcaaa tccactaaag catagaatga
    11281 cccccgtggg tagaaatatt accacccgtt tccctgaggt caacccggtt tccgatagta
    11341 gcggcaacac actagtatat acctcggtac agcggtttta caatgctatt cttatcagca
    11401 acaggggcaa cgagttttgg aacaatccgc taaacctcaa tcctcaactc tacgccgacg
    11461 gtgatattag aaccgtgggt ataacatcga acggtaatgt tcttttgctt tcccgaaacg
    11521 ataacgatat atacaatctg tactacagta catacaacaa ggaaaccaac tcctgggctc
    11581 caatcacaaa gttccccaag gagattaata cttccaaaag ctggcagacc tttgggtcac
    11641 tttccagaaa tggcgatact ctttacttca gcagtaaccg cgaaggtggc tttggcggtt
    11701 ttgatatctg gatgtccatc aaaacatcga atggttggtc ttcgcccata aatcttggca
    11761 aaaatgttaa caccccattc gatgagattg ctccattcct aaccgaggat gggaaacgtc
    11821 tatttttcag ctccaatggg cttaaaacaa tgggtggttt tgacatcttc tactccgatt
    11881 acgaaaacaa agcctggggc ataccaataa acgttggcta tccgctcaac accaccgacg
    11941 acgacacctt cttcttccct attaaaaatg ggaaagaagg ctactactca aagaattcgg
    12001 aaagcaaggg agatgaagac atttaccatg ttgccctgga tattaaatcg ccccgctagt
    12061 caatgaaagc atttagaatt tcggtattcc ttctgctgat agcttggctg ccgttctact
    12121 cctcaggcca aactaaaacc gaaattgacg aaacctatcg ggatgcaaac tcatacttct
    12181 actttgagga ttacgaggaa gcgctagcac tctaccaaaa aatttacaag ttccagcccg
    12241 acaactacaa cctcaactat agaataggct tttgttacct taacatccct ggcagtaaac
    12301 ataaatctat ccagttccta gaaaaagctg ttcaaaacac tactacaagg tacaacgagg
    12361 aatccatcac cgagcaacgt gctccaattg atgcaatatt ttacctgggt aacgcctacc
    12421 taacagtcaa taggattgac gacgctttaa aagcatacga aaaattttat agcttaacaa
    12481 agggcaaatc cgactgggac tttgattact taaaccatca gataaatgtt gccaaaaact
    12541 cactcaaaat tcaacgtaat ccggtggatt ttataatggc aaacctgggt gaaaaaatca
    12601 acgacagatt tccaaacttc aacgctgttg tatcgggcaa tggtaaagtt cttgcattca
    12661 caaccaagct aaagttttac aatgccatta atatttcccg taaaaacgaa aagaaccagt
    12721 gggaaaaacc agttaatatc actttagatt tacaaacgga cataagctgc tcaaccttat
    12781 gcctctcgta cgatggaaac gagctatact tgttcagaga cgataatcat gatggaaaca
    12841 tctatgtcac ccggtttaat gggaagaaat ggttaccaat taaaaagtta aaccccaata
    12901 tcaataccga ggcatatgaa acccacgcct cgttaagtcc cgatggtaaa cagcttttct
    12961 ttaccagcaa taggaaaggg ggatttgggg aacttgacct ttgggttagc acaagaacat
    13021 ctggcgatga ctgggggcca gctcgcaatc tgggcgcaac aattaactcg ccttttaacg
    13081 aaaacacacc gtttatcagc accgatggca ctacgctcta ttttagctcc gagggtcatg
    13141 aaaacatggg aggatacgac atttttgttt cgcagctttc agggaaaggc gattggacaa
    13201 cccctataaa tctgggatac cccttaaata ctactgacga tgacctgttc tatcacccca
    13261 tcgaggatgg agcaacagcc cttatagctc gctttgatga taattcattt ggtgaaaccg
    13321 atatttttga gatagaactt tttattccac gattcagaaa aagcattgta ccaaaaacag
    13381 ttgctcaaga acgcaccacc gataaaactt ttgcccggct aatagttgac accctttcaa
    13441 caaaaggcat ggcatccatt gatgccaatg aagccattac aaagattgat attagcggca
    13501 acaacacatt aaaactattc ctcaacggga aagcatacgg ggtaatccca tcaagaatag
    13561 aggagttggc tgaagctaaa agagtatcag aaactgagaa tgcagctaag gcttacaccg
    13621 aagagaccga caatcagaca cctagcccca tatcaagtaa ttcagctacg gatgctacct
    13681 ttaagaataa ttttggcgat actactccac gttcccagtg gccaaccgac acccctaccg
    13741 taaaacaaaa agcatttgag ctaaacaatg caagcattaa cgacgacagc tatttctctg
    13801 agatattgct tttgctaacc cctaatgaga aacaacaaga tattcttcct gttctgaaaa
    13861 ggaactggaa atttagtacc gaaaacctaa acgagaccat tatacatttt gcatcatcgt
    13921 ttaaaaccga caaagagctg aacaccatta tttatagttt aaccgaacta tacgataaaa
    13981 tcggaaatct aagcaatact caatcaggca aaaaggaaca aaaatttgcc aagaacaatc
    14041 gaactttaat cataaaggca tataataaac ttattaacaa agcctcaaaa cagcttggca
    14101 atgacctagc attggttatg cttaaaaaca aggagattgg aaatctgaca cagctcattg
    14161 aattatacag caagcaaaac cctgaaggct tcaatgcaaa tagagaggaa cttacactcc
    14221 tgttggctcg aatggcaatt agtgaatatg ttgcattgcc caatgagcaa aaaatatcgc
    14281 tctacaatag cagtacagga aagaccagtg ttgaaccttc gtatatttgg ctatgggtat
    14341 tgcttacact gatagcaatg ggggtgatta tttatctcgg aatagaatat tataaaaaga
    14401 aaacaagcta aaacgttgga caaggaatgg ccttccagcg ctttttaggt gttaacctaa
    14461 attcatatat catagatatt tcatgtgaac cacctgaggc caaaccaagt cgggatattg
    14521 taaaatcgta gctatacccc atgcttactt gctcccactt gtagccaacc ataaaagcta
    14581 aggcatcaac ccttcggcca acggagtttt tgggtaatcc cctccaccaa acaccaaaac
    14641 taatgggttg tgaatagaag tataatccca aatcgagctg gcggtaaata tcctgaacac
    14701 gataattaaa ggcaacagta aaggactcct caattctact gagcaaaaaa ccttttgtaa
    14761 tgtacctata tccaccatgc acggttatct tatatggcag cctggaatta tcgtcgtaga
    14821 aagtggccac gggtttgagc atatgatccc atgtaacacc aagccatatg ttatcggagt
    14881 atgccaggag cgaagaggaa acatcaatat cccatacagc atccttacca ggctgtgtaa
    14941 ctgaggttgg tggcataggt gtggaggtga gctggtcact aaaaatcagc ttactaaaat
    15001 caatggaaat ctgctgaagg aagaaagcca aaccaggtcg cag
//
LOCUS       DAOOWJ010000063        15034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_3990_length_15034_cov_7.392283, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000063 DAOOWJ010000000
VERSION     DAOOWJ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15034
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3990_length_15034_cov_7.392283"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(506..3313)
                     /locus_tag="PLW22_07400"
     CDS             complement(506..3313)
                     /locus_tag="PLW22_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU74567.1"
                     /translation="MKPVEGSNWHILLYSSNQSNCNEWVKELLNRPEFSHCKFLQVDS
                     LPDLNNVLSQRFNGVLLVHLTSNSYGLIKQAVSVRNDISPQVQVVALLDHDALNLDSA
                     KLLSTDIDDVVHCTSKEWLQATLLKYYFTSKADSCILPYAPFDRYSTHVDRIHSEFPF
                     GIVRVILKPDVYVEYINREAERILGFNLKQHKAQPELRNRFIPDERIKLILENAGTSN
                     EFIKRRFKISVGNDNYKWVEAIYIPGFKNNQSFQSIEVILRDVTREEIDKALQKAVYD
                     IARMTSTNFSLNRIFTVTHKIVSDLINIDNFYIALYNSENNEVSFPYFVDSTDIKTPN
                     PRRDGHGLTEYVIRTGKTLLCQPSCFDELIFKNEIDLIGTKPMAWLGVPLKFHGNILG
                     VMAVQHYTNINAFGESEQYILEYFSEQVARTIDYKRKADTVKLLSTAVEQSPVSVVIT
                     TLKPEIVYVNSTFTKVTGYEPHEVMGKNPRILQSGQTSKELYVELWDTLTSGSTWKGE
                     FINKRKNGEVFYEESIIVPITDELGKNAYYLSVKIDITDKRRLINELIIAKEKAEESD
                     KLKTAFLQNISHEIRTPMNAIVGFAEILESDHDDEDKISYYTGVIKQRSYDLLDIVNE
                     LLDISRIESGQLKAEFKPFDLNGIFTDLVQTFEGYKKRMEREEIELRLLPLPAHVSNL
                     IESDEPKLRQVLVNLIHNALKFTHSGYIEFGFHELTNGSIVFRVADTGIGIPHDMQDK
                     IFERFQKVSPKGVIYDGLGLGLTIVQSLANLLNGKIWLESEVGKGTTFYFSIPYKPIK
                     KVEEIEIEPVNPEVIKNLNLLVVEDDAFNIAYFNELFSGLSVNYHIENTGSSAIDHFE
                     QNSSYDVVLMDIKLPDMTGYEVIEEFKRINPTVPIIAQTAYAAESDRKKAFEVGCVDY
                     IAKPIFRDKLIGMLQKHLVKG"
     gene            complement(3332..3709)
                     /locus_tag="PLW22_07405"
     CDS             complement(3332..3709)
                     /locus_tag="PLW22_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU74568.1"
                     /translation="MENIKDLSNKTILVVEDDKFTSLIIQNFLAKTKAKIVTAYNGEQ
                     AVEQARAHNPDLIFMDLKLPGISGHEATRRIKEFLPNTIIIAQTAFVTDADRAEALDS
                     GCSDFLTKPLSSDKIYRTLYKFL"
     gene            complement(3714..4982)
                     /locus_tag="PLW22_07410"
     CDS             complement(3714..4982)
                     /locus_tag="PLW22_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74569.1"
                     /translation="MRVYISILSCTLFFGFAVTGQNLIRNGSFEKYYVCPDNYTVEYT
                     KRFLPNWVMPTKGTPDYFNRCSKEMVGVPQNFMGSIFPAEGDGFIGLVLLDTPDVKEE
                     IDYRDYVHSGPLAPVNITNANIKKPDTKRKVKPINYREYIQTQLVTPLQPNQLYRISF
                     KYALAQHSTFVSNRLGIAFTESPITQKDGVLPIKPAVIIDTAVLYCTPGIWVEFADTF
                     RTQGGEFFMTIGNFFPDSQSTYFPNDISSLNTTLQRAILTNQVAYYYLDNIRLEPVKK
                     EDVKQLSPRFIPFSMLKRHELSKIDTLCRYYALLDEVYFDIGQPGTKPRSLCQLYWLT
                     DFMTQNPNLGIELNGILHEIENDTVGASSRLKAMMEYLQEMGISGGRIRLRIFEDNKA
                     ISPKYRFYREGLPCVFYSSLVAVRFFTIKE"
     gene            complement(4991..5455)
                     /locus_tag="PLW22_07415"
     CDS             complement(4991..5455)
                     /locus_tag="PLW22_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74570.1"
                     /translation="MFKQIILSASLFIAAIVTVQAQCTGFHTIRECTPPDISDFKRFG
                     QSRSRMVEAKKTFTYQVVLFGGYDYKIGICTEKGYYPVQFRIINAEDNSVFYDNANDD
                     YVETVGFTVEKTKNVLFEITVLASKKEFKDATDSRVCAGVAIYWRKVPKTGF"
     gene            complement(5473..6363)
                     /locus_tag="PLW22_07420"
     CDS             complement(5473..6363)
                     /locus_tag="PLW22_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74571.1"
                     /translation="MKQYRFYFVVLAMLFAAMFYSCSGGKTNQADVNTELSEDTLVQD
                     QMEIMLPTPSEVLSIVLTSGIKYSDSYMAPLGIEKKAVMVKHKALILGVYFADFAYTN
                     YYNQRNASSEYFKALQELTRDLGIGSILNDAYFKRFDANLNNPDSLDAIFEDFSQNAY
                     NTILESGNRELLSMIGIGAAIEVLQIGINSIENAANPDEAIATVIDQSAVFDNYYNNF
                     IAYNSSKPEFKSLTEDVTEIFKFFKEKLSSAGNESVVTDSKEHFTITGGDTTKLSKAD
                     LAQLRNMVANARKKLIDLKY"
     gene            complement(6643..7854)
                     /locus_tag="PLW22_07425"
     CDS             complement(6643..7854)
                     /locus_tag="PLW22_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU74572.1"
                     /translation="MNRGGYTYIFYENYNIPIFFLTSIIASMFLGMIVTSDSVIKDAN
                     INKREAFLFLNRKAYYNSKVLFYFGLTILQTLIYTAISIYILKVKGMFLPIWVILILM
                     GFFGNIAGLIVSTIFKSLSAAYLIVPFLVIPQIILSGITIPFDRMNHRLANPEVVPVI
                     GIVSPSMWGMEALLVHQFKNNAYERHFFEIDMAESQSRIQSQYLIPKLFQLIQSYKQT
                     DSVNRLRLKHLIKSGLGQLNIDIAEFPQPNTAEFRKLEIVLHELQQKQQQNYSRIIRK
                     KDQITTKLQSQFYSAEAFLEFKQMHTNRGVDDLTLARKNITAIQVVENRLVQTIDPVF
                     KIPTNRWGRAHFLAPAKRIANQLFDTFTFNVVVLLVFLVSIYLILLFDILPKLSNSMV
                     QLKLFKLVKGF"
     gene            complement(7811..9649)
                     /locus_tag="PLW22_07430"
     CDS             complement(7811..9649)
                     /locus_tag="PLW22_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU74573.1"
                     /translation="MNEFLVNSQVNLFAHIVLNGDASLQRLAEIYTEVLFTRLVNPRI
                     GKKQKEELKQVVRNSKQANSSIDVKKICSKLNSESSIQQRLILLLNLLEFGLFVQKNA
                     ILLSKSNSIAGKIKQIADELKISAETYSLCSSFVSGKLYEVHDRRNILIVKSVSPELE
                     GFHFVKREQIDGYFVFVYFPDVSTIFFRYQGKENLQLNSKPIQPKAIYSFQSGSVISQ
                     NGKPLLYYSKVIGQFKGLNQHESITLNLKNVEFTYPRSNFGLHNLSLTATSGELVGIM
                     GGSGVGKSTFFNLLNGSLTPQRGKISLNGVSYQDGLTQIQMLIGFVPQDDSLFENLTV
                     FENLYYTARLALGNLTVEQTKELVNAKLAEFGLYHIRNLKVGSSLSRNISGGQRKRLN
                     IVSEIIREPKLLLVDEPTSGLSSADSLRVMSLLKEQALSGRLVLVNIHQPSTEIYRLF
                     DKILVLDQGGYMVYYGNPLEAVRYFKMHAGRIDADEVECSVCKNIKSDEIFDIIDEKQ
                     VDEFGQLTENRKVKPIVWSSRFIPTDEGETTINPLSESKSIKTGWVKQLEVFLTRLTL
                     SKLRDIEFFIFAFVIPVLLAAVIAFFSKYSFPNEQGGVHLHLLRKL"
     gene            complement(9656..10300)
                     /locus_tag="PLW22_07435"
     CDS             complement(9656..10300)
                     /locus_tag="PLW22_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74574.1"
                     /translation="MKLNDYSSHSEILRQVGFESRTLLNGITGPIQLIRSLSNDPNLL
                     EILHILELSTVRFEKFSLRSQLLSDLLNPSMQVSNERFDLVDLLKHAVLELNDFINFF
                     GVIFELSKDLSSFEVEADRDIIYQSVLIVFEQFMSVLESGSAIKVDCEEHSQKISITA
                     FDNGFVADKFNQFSENQSVEIDVTLFVHSLKKFSIPFTVYKNNGHTVMEIARQQ"
     gene            complement(10351..11637)
                     /locus_tag="PLW22_07440"
     CDS             complement(10351..11637)
                     /locus_tag="PLW22_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOU74575.1"
                     /translation="MLRKLPLYVKILIGMFLGLAFGFLVITFGIQHFATDYIKPFGTI
                     FLNLLKLIAMPLIFASLVSGVSGLSDVSRLSKLGIKTISFYIVTTIIAVTIGLVLVSV
                     IRPGSYVSEAKRVELLEKYSFDLAKRQQAAEGIAQQSPLQFLVDIVPENVVNAASNNT
                     SMLQVIFFAIMFGVALILVPHSKVAIVKDFVAGLNEVILKIVDIVMLFAPFGVFALMV
                     TLLVDFVGTDLGSSVQLFTSLGLYALTVILGLLLIITLVYPTFLKLYAKINFKKFYRS
                     IFPAQLVAFSTSSSAATLPVTMEQVEKELNVPNEISSFVLPVGVTINMDGTSLYQAVA
                     TVFIAEVFGVELTLVQLLTILITATLASIGSAGVPGAGIVMLIIVLNSVGLPVEGLAL
                     ILAIDRPLDMLRTTVNVTGDAMIATIIAKSESKLGE"
     gene            complement(11776..12300)
                     /locus_tag="PLW22_07445"
     CDS             complement(11776..12300)
                     /locus_tag="PLW22_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74576.1"
                     /translation="MKRKLLGMVLMALAIVTSCKNDNDDQDINIIRFNDGQFTLYRGY
                     SHKYSDALETGATPFVINLLGEGVSYSTDAGKFTGTGSLVTGYFYSENIAEVKNGLYT
                     IDIFSQKGKYTADSCKIYYNYDFAQDTGKIYTIKAGTFDVVNLGRLMSYKIDIQTADY
                     IHFTGEFRGTVDPL"
     gene            complement(12584..13078)
                     /locus_tag="PLW22_07450"
     CDS             complement(12584..13078)
                     /locus_tag="PLW22_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU74577.1"
                     /translation="MNVSKNKVVSISYELKVDGDLIDAAQADNPLVFLYGHGQLLPLF
                     EDNIKDLAEGDSFEFMIPSKDGYGEVNDQAIVELPKEIFVIDGELQEDLLVIGNRLPM
                     RDSEGNALDGTVVEVTDNSVVMDFNHPLAGKDLYFTGKVENIREATAEEISHGHVHGM
                     GHDH"
     gene            13289..13489
                     /locus_tag="PLW22_07455"
     CDS             13289..13489
                     /locus_tag="PLW22_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOU74578.1"
                     /translation="MKGKVKWFDAAKGYGFIQTEEGNDVFVHYTGIQREGFKRLDEGQ
                     AVSFEISEGKRGPQATNVVVLE"
     gene            complement(13544..14137)
                     /locus_tag="PLW22_07460"
     CDS             complement(13544..14137)
                     /locus_tag="PLW22_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74579.1"
                     /translation="MLKKSIFLVIFVSVSTLVFSQRKHGDFQRFSDRVYFGGSLGLAI
                     GNRITQIDVLPQGGIWIFPQWSVGLGGRYTYRRERFSFDPSSTSIKPVTTTIWGYSGF
                     TDILPIPDFNEAFGVKLHGGFVIHGEFENLYLDRNFFDITSTQGMGWIRMYMVGGGWR
                     QRIGDRAAVNFLVLWDLTDNRYSPYTSNPILRFSITF"
     gene            14226..14933
                     /locus_tag="PLW22_07465"
     CDS             14226..14933
                     /locus_tag="PLW22_07465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU74580.1"
                     /translation="MNIVITGASQGIGYATAIELAQKGNNTIFCIARNGHNLEKLQNE
                     VAKINPASTVIPVVYDLTKLCQGTEDIGSIIRKHIDKVDILINNAGLLYVKPLTELTY
                     EQISSMLEVNYLAPILLIKQLIPLMDTGSHVVNISSMGGFQGSAKFPGLSVYASSKAA
                     LASATENLAEEFKSTGITFNCLALGATDTEMLRNAFPGYKAPLTAQEMASFIAYFAQN
                     GNRFFNGKILPVSVSTP"
BASE COUNT         4599 a         3029 c         2949 g         4457 t
ORIGIN      
        1 tgtgtgtcgc ttaaatctat ttctaacctg tagacttatt tcaggactag acaaggataa
       61 aggattagtt gatggatgct ggatgattgt tgatagtttg gtgttgcctc gtcttatcgt
      121 gtcatgttga gttcccgata tatcgggata gattccaccg aagcatatct tttctccttt
      181 taccctttga tgagattcct cgactagctc ggaatgacaa cactattggc tccctcactc
      241 cctttgtcat gctgagcacc gccgaagcat ctcatttaac ctccaataca tttatacctt
      301 ttgattagtt tccaagcagt cgctttttgg aatgtttttc cccatgcgat gcaacgtttc
      361 tttactgctt tttagattat gcacagcgag catcgctaat ttttttttat atatatcaat
      421 accctggttt aaaaccctgg tttaaagcga tttgccattt caggacgaat attgcacacg
      481 tctggagacg cgcgcaaaca atgtactaac ctttaaccaa atgcttttgc agcatgccaa
      541 ttagcttatc gcggaatatg ggtttggcaa tgtaatccac acatcctacc tcaaaagctt
      601 ttttcctatc gctctcggct gcgtaggctg tttgggcaat gattggtaca gtagggttga
      661 ttcgcttgaa ctcctcaatt acctcgtacc cggtcatatc gggaagtttt atgtccatta
      721 gaactacatc gtacgatgag ttctgttcaa aatggtcaat tgcactacta ccggtattct
      781 caatatgata gttaacactt aatccactaa aaagctcatt gaagtaggca atgttgaaag
      841 catcgtcctc cactaccaaa aggttcagat ttttaatcac ttccggattc acaggttcaa
      901 tctcaatttc ctcaactttc tttatgggct tgtaaggtat tgagaagtag aatgtggtac
      961 ctttgccaac ctcagactca agccatattt ttccgttgag caggttagcc aaactttgaa
     1021 caatggtaag tccaagtcca agcccgtcat aaatcactcc cttagggcta accttttgga
     1081 aacgctcaaa tattttatcc tgcatgtcgt gtggtatgcc aattcccgta tcggcaaccc
     1141 ggaatacaat tgaaccgttg gtaagctcat ggaatccaaa ctcaatgtaa ccactatgtg
     1201 taaactttaa ggcattatgg ataaggttaa caagtacctg gcgtagtttt ggctcatcgc
     1261 tttcaatcag gttcgatacg tgagcgggta aaggtaatag ccttaactca atttcctcac
     1321 gctccatacg ttttttgtaa ccctcaaagg tttgaaccag atcggtaaaa ataccattta
     1381 aatcaaaagg cttaaattca gccttaagct gtcccgattc gatacgggaa atatcgagta
     1441 gctcgttaac aatatcaagc aaatcgtatg agcgctgttt tattacgccc gtgtagtagc
     1501 taatcttatc ttcatcgtcg tggtccgatt caagaatttc agcaaagcca acaatggcgt
     1561 tcatgggtgt acgaatctcg tggctaatgt tctgcaaaaa agccgttttg agtttatcgc
     1621 tctcctctgc tttctccttt gcaattataa gttcgttaat taatcttctt ttgtcggtta
     1681 tgtcaatttt cactgaaagg taataggcgt ttttaccaag ttcatcggta ataggaacaa
     1741 ttattgattc ctcataaaaa acctcaccat tcttccgctt attgataaat tcacccttcc
     1801 aagtgctacc ggaagtaagt gtatcccaca attcaacata aagttcttta gatgtttgtc
     1861 ccgactgtaa aatacgtggg tttttaccca taacctcatg gggttcataa ccggttacct
     1921 tggtaaaggt agagtttaca tatacaattt cgggctttag agtggtaatt acaaccgata
     1981 cagggctttg ctcaacggct gtggaaagta actttacagt atcggccttg cgtttgtaat
     2041 caattgttcg tgctacctgc tcggagaaat actccaaaat gtattgctca ctctccccaa
     2101 aagcattgat attggtatag tgctgaaccg ccattacccc gagtatatta ccatggaatt
     2161 taaggggaac tcccaaccat gccattggtt ttgttccaat taggtcaatc tcatttttaa
     2221 aaattagctc atcgaagcaa cttggttggc aaagaagagt ttttcccgta cgaattacat
     2281 actcagttaa tccatggcca tccctacggg gatttggggt tttaatatcg gtagaatcga
     2341 caaagtaagg gaacgatacc tcattgtttt cggaattata aagggctata tagaagttat
     2401 cgatattaat taaatcggaa actattttat gggttaccgt aaaaatcctg ttcaacgaga
     2461 agttggtgct ggtcattcgg gcaatatcgt aaaccgcttt ttgcagcgcc ttgtcaattt
     2521 cttcgcgggt aacatccctt agaataacct caatggactg gaatgactgg ttatttttaa
     2581 aaccaggaat ataaatagcc tccacccact tataattatc gttaccaacg cttatcttga
     2641 atcgacgctt aatgaattca ttgctggttc cagcgttttc aaggattagc ttaatgcgtt
     2701 catcagggat aaacctgttc ctaagctctg gttgtgcttt atgctgctta aggttaaaac
     2761 ctaaaatgcg ctctgcctcc ctgttaatgt attcaacgta aacatctggc ttaagaataa
     2821 ccctgacaat accaaaggga aattcgctat gaattctatc gacatgggtg ctataccgat
     2881 caaatggagc gtagggtaat atgcaactat cggccttact ggtaaagtaa tattttaata
     2941 gtgttgcctg taaccattct ttactagtgc aatgtaccac atcgtcaata tccgtactga
     3001 gcaatttagc agaatcgagg tttaaggcat catggtcgag caaggcaaca acctgaactt
     3061 ggggagaaat atcattcctg accgacactg cttgcttaat taaaccgtaa ctattagaag
     3121 ttaaatgcac caataaaacg ccgttgaaac gctggcttaa aacattattt aaatcgggaa
     3181 gggaatcaac ctgtaaaaat ttacaatgtg aaaattcagg gcgattaagc aattccttta
     3241 cccattcatt acaatttgac tgattcgatg agtaaagcag tatatgccag ttactacctt
     3301 caacgggttt catcaagtaa aatgttaagg gttacagaaa tttgtaaaga gtacggtaaa
     3361 tcttatcgct gctaagcggt ttggttaaaa aatcggagca gccagaatca agagcttcag
     3421 ccctatcggc atcggtaaca aaggcggttt gagctataat gatagtgttt ggaaggaact
     3481 cctttattct tcgggttgcc tcatgcccac ttatgcccgg aagcttaagg tccatgaaaa
     3541 ttaaatcggg gttgtgagcg cgtgcctgct caaccgcctg ctcaccattg taagcagtta
     3601 caattttagc ctttgttttt gcaaggaaat tctggataat tagagaggta aacttatcgt
     3661 cctcaaccac cagaatggtt ttattgctta aatcttttat attctccatg gcactattct
     3721 ttaatggtaa aaaaacgaac cgctacaagc gatgagtaga aaacgcatgg caaaccctcg
     3781 cgataaaatc gatatttggg cgaaatggct ttgttgtcct caaaaattct caaccgtatc
     3841 ctgccaccac taatgcccat ttcctgcaaa tactccatca tggctttaag ccggctactt
     3901 gcaccaactg tatcgttttc aatctcgtga aggataccat taagttcaat tcctaagttg
     3961 gggttttgag tcatgaaatc agtaagccag taaagctgac aaaggctacg gggtttggtg
     4021 ccaggttgtc caatatcaaa gtaaacttca tcgagtagcg cataatatct acatagggta
     4081 tcaatttttg aaagttcatg ccttttaagc atgctaaagg gaatgaagcg aggcgagagc
     4141 tgctttacat cctctttttt aacaggctca agcctgatat tatctaggta atagtaagca
     4201 acctggttgg tgagtatggc gcgctgaagg gttgtgttta aggaactaat atcgtttggg
     4261 aaataggttg attgactatc agggaaaaag ttgcctatgg tcataaagaa ctcaccgccc
     4321 tgggttctaa aggtatcggc aaattcaacc caaattcccg gtgtgcagta tagtaccgca
     4381 gtgtcaataa taacagctgg tttgattggc aggacaccat ccttttgagt tattggactt
     4441 tcagtgaaag ctatgccaag cctattcgat acaaatgtag agtgctgcgc aagggcatac
     4501 ttaaagctaa tgcggtatag ctggttaggt tgcaggggtg taaccaactg cgtttgaata
     4561 tactccctgt agttaatagg tttaacctta cgcttagtat cgggcttttt aatattagcg
     4621 ttagtaatgt taacaggtgc aagggggccg ctatgtacat agtcacggta atcaatttcc
     4681 tccttcacat caggggtgtc gagcaacact aagccaataa agccatcgcc ctcggccggg
     4741 aaaatactac ccataaagtt ctggggtacc ccgaccatct ccttactaca acggttaaag
     4801 taatcgggag tacccttggt aggcattacc caattgggta aaaaacgttt tgtgtattca
     4861 accgtatagt tatcgggaca aacatagtat ttctcaaagc tgccattcct aatgagattc
     4921 tgccccgtta cagcaaaacc gaaaaataat gtgcatgata gtatggatat atataccctc
     4981 atagcttatg ctaaaaacct gttttaggga ctttgcgcca gtatattgct acaccggcac
     5041 atacccttga atcggttgcg tccttaaatt cctttttgct tgctaatacg gtaatctcaa
     5101 aaagaacgtt tttggtcttt tcaactgtaa atcctactgt ttctacgtag tcgtcgtttg
     5161 cattatcgta aaaaactgaa ttatcctccg cattaattat cctaaactga actggataat
     5221 aacccttttc agtacagata ccaattttat aatcgtaacc accaaaaagt acaacctggt
     5281 aggtaaaggt ttttttggct tcaaccatac ggcttcggga ctggccaaac cgcttgaaat
     5341 cactaatatc tggtggagta cattctctaa tagtatgaaa acctgtacat tgagcctgta
     5401 ccgttacaat agccgctata aatagtgaag ctgaaagtat aatttgctta aacatagcag
     5461 aaaggtttta ggttaatact taaggtcaat taacttttta cgagcattgg caaccatgtt
     5521 gcgcagctgt gccaaatcgg ctttgctaag ctttgtagta tcgccaccag ttatggtgaa
     5581 atgttccttg ctatcagtta ctaccgattc gttaccagct gaagatagtt tctccttgaa
     5641 aaacttgaaa atttcagtaa catcctcggt taaactttta aattcaggtt tactgctatt
     5701 gtatgcaata aagttattgt agtaattgtc aaaaacagcg ctctggtcaa taaccgtagc
     5761 tattgcttca tcggggtttg ctgcattctc aattgagtta atccctattt gcaatacctc
     5821 aatggcagca ccaataccaa tcattgagag tagctcccta ttgcccgatt caaggatagt
     5881 attgtatgcg ttttgcgaga aatcttcgaa aatggcatcc agtgagtctg ggttattaag
     5941 gtttgcgtcg aaacgcttaa agtaagcatc gttcagtatg gagcctatac ccaaatcgcg
     6001 agttagctcc tgtaaggctt taaagtattc cgaggaagca ttcctctggt tgtagtaatt
     6061 ggtataggca aagtcggcaa agtacacgcc taaaatcagt gccttgtgct taaccataac
     6121 cgcttttttc tcaataccaa ggggtgccat atagctatcg gaatacttaa ttcccgatgt
     6181 aagtacaatg ctcagcacct ccgatggcgt gggtagcata atctccattt gatcctgaac
     6241 taaagtatcc tcgctaagct cagtattaac atcagcttga ttggttttac ctcctgaaca
     6301 ggagtaaaac attgctgcaa acagcattgc taaaaccaca aaataaaatc tgtattgttt
     6361 cataaagcaa agtattaggt tcaatatttt catgttttac agttatcgcc aatttaagtt
     6421 tttccaattt aaaaagcaag tgataaaaaa caagaatgtg ttaaacgttc aaaaaattag
     6481 cattggtaac aattcgtttg ttttgtaatt acacaaaaga tgcaatagga ctgataaagg
     6541 ttaaaggata aaggttaaag gataaaggat taaagataaa ggattaagga ttaaggatag
     6601 caatttgata taatagggac gagggtgcaa taataatatt ccctaaaaac cctttactag
     6661 cttaaatagc ttgagttgaa ccattgaatt ggagagtttt ggtaaaatat caaatagtaa
     6721 gatgaggtaa atacttacaa gaaaaacaag aagcacgaca acattaaaag tgaaggtatc
     6781 aaatagctgg tttgcaatgc gcttagctgg agcaaggaag tgtgctctac cccatctatt
     6841 cgtgggaatc ttaaaaaccg gatcgattgt ttgaaccagc ctattctcaa ccacctgaat
     6901 ggctgttata ttcttgcggg caagagtcaa atcatctact cccctattgg tgtgcatttg
     6961 cttaaactcc aaaaaggctt ccgcactgta gaactggctc tgtagctttg tagtaatttg
     7021 atcctttttt ctaatgatcc tcgaatagtt ttgttgttgt ttttgctgta gttcatgaag
     7081 tacaatttca agcttacgaa actcagcagt gttaggctgt ggaaattcag caatatcaat
     7141 atttagttga cctaaaccgc ttttaatgag atgtttgagc ctaagccggt taacgctatc
     7201 ggtttgttta tagctctgaa taagctgaaa aagttttgga attaggtatt gcgattgaat
     7261 acggctttgg ctttcggcca tatcaatttc aaaaaaatgc ctttcatagg cattattctt
     7321 gaactgatga acaagcaagg cttccattcc ccacatggag ggagaaacta ttccaatcac
     7381 gggaacaacc tcaggatttg ctaaccgatg gttcatccta tcaaaaggaa tggttatacc
     7441 gcttaggata atctggggaa taaccaggaa tggcacaata agataggcag ccgataacga
     7501 tttgaaaatg gttgaaacaa tgaggccggc gatattcccg aaaaatccca ttaaaatcag
     7561 tatcacccaa ataggtaaaa acattccctt taccttgagt atataaatag atattgccgt
     7621 gtatattagc gtttgaagga ttgttaagcc gaagtaaaaa agcacctttg aattatagta
     7681 ggctttgcgg ttcaggaaaa gaaaagcttc acgcttattg atattggcat ccttaataac
     7741 actatcggat gtaactatca tacccaagaa catggacgca ataatactgg taagaaagaa
     7801 aatgggtatg ttatagtttt cgtaaaagat gtaggtgtac ccccccctgt tcattcggaa
     7861 acgaatactt gctgaaaaag gctataacgg cagcaagtaa aacgggtata acaaaagcga
     7921 aaataaaaaa ttcaatatcg cgtaacttgc taagggtaag ccttgtgaga aatacctcaa
     7981 gttgcttaac ccagcctgtt ttaatcgatt tgctttccga taatggattt atggtggttt
     8041 caccttcatc tgtaggtatg aagcggctgc tccaaactat gggctttact tttcggtttt
     8101 cggtaagctg gccaaattca tcaacctgtt tctcgtcaat tatatcgaaa atctcatcgg
     8161 attttatgtt cttgcaaacg ctgcactcca cctcatcggc atcaatgcgg ccagcatgca
     8221 tcttaaagta acggacagcc tcaagcggat taccgtagta caccatatat ccgccctgat
     8281 caagcaccaa aatcttatca aataacctat aaatttcagt ggagggttga tggatgttta
     8341 caagcactaa ccttccagat agcgcttgct ccttgagcaa cgacataact ctaagcgagt
     8401 cggccgatga caagcccgat gttggctcgt cgacaagcaa caactttggc tccctgatga
     8461 tttcggaaac tatgtttaac ctcttgcgct gtccaccact aatattacgc gaaagcgaac
     8521 tcccaacttt taggttacga atatggtata gtccaaactc agcgagctta gcatttacta
     8581 gttccttggt ttgctcaaca gttaaattac ctagggcaag ccgtgcagta tagtataaat
     8641 tctcaaaaac ggttaggttt tcaaaaagcg aatcgtcctg gggaacaaaa cctattaaca
     8701 tttggatctg ggtcaaacca tcctgatagc taacaccgtt taaagaaatc ttaccccttt
     8761 ggggtgtcaa tgagccattg agaaggttaa agaaagttga ctttccaacc ccacttcccc
     8821 ccattattcc caccagctca cccgacgtgg cagttagcga caagttgtgt aatccaaaat
     8881 ttgaacgggg ataggtaaac tcaacatttt ttaggttcag ggttattgat tcatgttggt
     8941 ttaatccttt aaactggcca ataaccttac tatagtacaa aagcggtttg ccattttgtg
     9001 aaataactga cccactctga aatgagtaaa ttgcttttgg ctgtatgggc tttgagttta
     9061 gctgtaggtt ttcctttccc tggtaacgga aaaagattgt agaaacatcg ggaaagtaaa
     9121 caaatacaaa atacccatca atctgttccc tttttacaaa atgaaagcct tccaattcag
     9181 ggcttaccga ctttactatt aggatattcc tacggtcgtg tacttcgtaa agcttgcccg
     9241 aaacaaagct gctgcataag ctataggttt cagccgatat ttttaactca tcggctatct
     9301 gttttatttt accagcaata ctatttgatt ttgatagtag aattgcattt ttctgaacaa
     9361 ataagccaaa ttcaagaaga tttagaagaa gtataagcct ttgctgaatt gaactttcac
     9421 tatttaattt gctacaaatc tttttaacat caatgctact attggcttgc ttgctattcc
     9481 tgactacctg ttttagctcc tctttctgtt ttttcccaat cctgggattt acaaggcggg
     9541 taaaaagaac ttcagtgtaa atttctgcaa gcctttgcag gctggcatcg ccattaagca
     9601 ctatatgggc aaaaaggtta acctgtgaat ttaccaaaaa ctcgttcatg gctttctatt
     9661 gttggcgagc aatttccatt acagtatggc cattattctt gtagacggta aagggtatgc
     9721 tgaatttttt aaggctatgc acaaacaggg taacatcaat ctctaccgac tggttctcgc
     9781 taaactggtt aaacttgtcg gcaacaaacc cattgtcgaa tgctgttatg gaaattttct
     9841 gggaatgctc ttcgcaatca accttgatgg ctgaaccgct ttcaagaact gacatgaact
     9901 gctcaaaaac tatcagaact gactggtaaa taatatctct atcggcttca acctcaaaac
     9961 ttgataagtc cttgcttaat tcaaaaataa cgccaaagaa gttgataaaa tcatttagct
    10021 ccagtacagc atgttttagt aaatcgacca agtcaaatcg ctcgtttgat acttgcattg
    10081 atgggtttag taaatcggaa aggagctgcg agcgtaacga gaatttttcg aaccttaccg
    10141 tgcttaattc caggatgtga agtatttcaa ggaggtttgg gtcgttgctt agtgaacgta
    10201 taagctgaat tgggccggta atgccattaa gtagagttct tgactcaaac ccaacctggc
    10261 gtaaaatctc ggagtgactt gagtaatcat ttagtttcat ggctaactta tgtcagccta
    10321 aggtaataaa aaaagctgaa agttgttcat ctactcacca agtttactct cgcttttagc
    10381 aattattgta gcaatcattg catcgccggt tacatttact gttgtacgaa gcatatcgag
    10441 tgggcggtca atggcaagta ttaaagccaa accctcaacg ggcaagccca cagagttcag
    10501 aacaataatt aacattacaa taccagctcc gggcacaccc gctgagccaa ttgatgccaa
    10561 agttgccgta attagtatgg ttagaagttg tactaaagta agttcaaccc caaaaacctc
    10621 ggcaataaaa acggttgcaa cagcctggta aagacttgtc ccatccatat ttatggtaac
    10681 ccctaccggc aatacaaagc tcgatatttc attgggaaca ttcaactcct tttccacctg
    10741 ctccatggta accggtagtg tggcggcgct cgagctggta gagaatgcca ctagctgagc
    10801 aggaaaaatg gatcggtaaa atttcttaaa gttgatttta gcgtaaagct ttaaaaatgt
    10861 ggggtatact aaggtgataa ttaataataa tccaagtatt acggttaaag cgtataaccc
    10921 cagcgaggta aaaagttgaa ccgatgaccc taaatcggtt cctacaaaat cgactaaaag
    10981 agttaccatt aatgcaaaca caccaaaagg ggcaaaaagc ataacaatat ccactatttt
    11041 gagaattacc tcatttaacc ccgcaacaaa atcctttact atagccacct ttgaatgggg
    11101 aacaagaatt aacgcaacac caaacattat ggcaaagaaa atgacctgca gcatggaagt
    11161 gttattggat gctgcgttta caacattctc tggaactata tccacaagga attgcagagg
    11221 tgattgctgt gcgatgccct cggcggcttg ttgccttttg gctaaatcga acgagtattt
    11281 ttcaagtaat tcaacccttt ttgcctcact aacatagctt ccggggcgaa taacactcac
    11341 taaaaccagc ccaattgtta cagcaattat tgtagtaaca atgtaaaatg aaatggtttt
    11401 tattcccagc tttgataacc gactaacatc gcttaagccg ctaaccccac tcaccaacga
    11461 tgcaaatatt agcggcatgg caataagctt aagcaggttc aggaaaatgg ttccaaaagg
    11521 cttgatatag tcagtagcaa aatgctgtat tccaaaagta attaccaaaa atccaaatgc
    11581 caagccaagg aacatgccta ttaaaatctt gacgtaaagg ggtaatttgc gaagcataga
    11641 caggaattta aggataaagg ataaaggata aaggataaag gttaaaggat aaaggataaa
    11701 agtttaaggt ttaagtttaa aatttcagat ttcagatttc agatttcaat aatattcaaa
    11761 tttctagttt catagctaaa gaggatcaac tgtgccccta aactcgccgg taaagtggat
    11821 ataatcagcg gtttggatat caatcttgta gctcattagc ctacccaggt taaccacatc
    11881 gaatgtacca gccttaatag tgtatatttt gccggtatcc tgtgcaaaat cgtagttgta
    11941 gtatattttg catgaatcgg cagtatattt tcctttttgt gaaaagatgt cgatggtgta
    12001 aagaccattc ttaacctcgg caatgttttc tgagtaaaag tatccggtta ctaaggatcc
    12061 agtcccggta aatttaccgg catcggtgct gtagcttact ccctcaccaa gcaggtttat
    12121 aacaaatgga gttgcgcctg tttcaagggc atcgctatac ttatggctat agcctcggta
    12181 aagggtgaac tgtccatcgt taaacctgat gatatttatg tcctggtcgt cattatcgtt
    12241 tttacagctg gttacaatgg ccagcgccat cagaaccatt cctaaaagtt tacgtttcat
    12301 acgtacaaaa tatttatatt attaattact aaatcattac tctcttgctt ttaacaaaca
    12361 ttatcgagcg gatggaacac ccatagtttg actttgtcga gctcgcaaaa aacgctcggt
    12421 tgttcatctg tgtttgtgca agctaccttc catgggcgtt tcataatctg aaaatattag
    12481 gcaagtaatt taatattact gctaaaatag taaaaatgtt acataatgta aaaaaggagg
    12541 tgaataattc accccctttt acttacaaaa gctgaaatat ctgttagtgg tcgtggccca
    12601 ttccatgcac atgaccatgg ctaatctctt cggcagtagc ttcgcgaatg ttctcaacct
    12661 taccagtgaa gtaaaggtcc tttccagcta gggggtggtt aaaatccata accacggagt
    12721 tatcggtaac ttcaaccact gttccatcca gcgcatttcc ctcggagtcg cgcatgggta
    12781 agcggttacc aatcaccagg agatcttcct ggagctcacc atcaataaca aaaatctcct
    12841 tgggtagctc aacaatggcc tggtcgttaa cctcaccgta accatccttg cttggaatca
    12901 taaattcaaa ggaatcgcct tctgcgagat ctttaatatt atcctcaaaa agaggcaaga
    12961 gctggccatg cccatagagg aatacaagtg gattatctgc ctgtgcagca tcaatcaaat
    13021 cgccatcaac ctttagttca tagctaattg atacaacttt gtttttggaa acgttcatgt
    13081 aaattgtttt aaaatattaa ttcatcaaag aaactacttt cttttggtat aaacaagcgt
    13141 gtaaggttaa ttgttcgcat ttaaggttga taaattttgg tcataggtaa acaaaaaaca
    13201 taaaatgatg ttgtttattc aaataatagt tacatatttg taatgtttta aaacaatcaa
    13261 tttattcaca atttattttt aacgtaacat gaaaggtaaa gtaaaatggt ttgatgcagc
    13321 aaaaggctat ggctttattc aaaccgaaga aggtaacgat gtattcgttc actacacagg
    13381 aattcaacgc gaaggtttta aacgcttaga tgaaggtcaa gccgttagct tcgaaatttc
    13441 tgaaggcaaa cgtggtccac aggccacaaa tgtagttgtg ttagaataac atttattcct
    13501 aacataataa aagaaaaggc ctctgtttga ggcctttttt attttaaaac gtgatagaga
    13561 accgaagaat agggtttgaa gtgtaaggcg aatagcggtt atcggttaaa tcccatagaa
    13621 ccaaaaagtt aacggctgcc ctatcgccta tccgttgacg ccagcctcct cctaccatat
    13681 acatccgtat ccatcccatg ccttgggtag aggtaatgtc aaaaaaatta cggtcaaggt
    13741 aaaggttctc gaattcaccg tgaattacaa aaccaccatg aagcttaacg ccaaaggctt
    13801 cattaaaatc ggggatgggt aaaatatcgg taaaaccaga atatccccat attgtagtag
    13861 ttaccggttt tatactggtg ctcgaggggt caaagctaaa cctttccctg cgataggtat
    13921 aacgcccacc tagcccaacc gaccattgtg gaaaaatcca aatgccaccc tggggcaata
    13981 catcaatttg ggttatgcga tttccaatag ctaggcctaa actaccccca aagtatactc
    14041 tatccgaaaa tcgttggaaa tcgccatgtt ttcgttgcga aaagacaagg gtgcttaccg
    14101 agacaaatat tactagaaag atagattttt tcagcataat actgatgttt acttactaat
    14161 atgaacacaa tattagccat ttatgtttaa ttggttaact ttatgtctgt taaaaaagat
    14221 tagcgatgaa catagttatt actggtgcct cgcagggcat aggttatgca actgcgattg
    14281 aactagccca aaaaggaaat aacaccattt tctgtatagc ccgaaacggg cataacctag
    14341 aaaaactaca gaatgaggtt gcgaagatta acccagcctc aaccgttatc cctgtagttt
    14401 acgacttgac aaaactctgc caaggaactg aggatattgg ctcaatcata agaaagcata
    14461 tcgacaaggt tgatattctg ataaataatg caggattgct ttatgtaaaa ccccttactg
    14521 agcttacata cgaacaaata agcagtatgt tagaggttaa ttaccttgcc cctatccttt
    14581 tgataaaaca gttaatacca ttaatggata ctggtagcca cgtggtaaac attagcagta
    14641 tgggtggatt tcagggaagc gctaagtttc ctggcctatc ggtttatgct tcatcaaaag
    14701 cagccctagc ttctgcaacc gagaacctag ccgaggagtt caaaagcaca ggtattactt
    14761 ttaactgcct agctctaggc gctacagata ccgaaatgct tcgcaatgct tttccgggct
    14821 acaaggctcc cttgaccgct caggaaatgg ctagctttat tgcatacttt gcacagaacg
    14881 ggaataggtt ctttaatggg aaaatactgc ctgtaagcgt tagtacaccg taagtaagaa
    14941 aggataaagg ataaaggata aaggataaag gttaaaggtt aaaggttgac ccgtcctgaa
    15001 aaatgtctac acaaacttat gtgtatggac aaaa
//
LOCUS       DAOOWJ010000064        14811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4076_length_14811_cov_8.932638, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000064 DAOOWJ010000000
VERSION     DAOOWJ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14811
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4076_length_14811_cov_8.932638"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1450
                     /locus_tag="PLW22_07470"
     CDS             332..1450
                     /locus_tag="PLW22_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74581.1"
                     /translation="MAYYINLFSPDTYQKFSDSDKSISGVSDKLKDVASTIKPGDKLI
                     CYVTKLSRWVGVLEVTSNYFINDSPIFTQTADPYVVRFNVKPIIWLPLENAIPVHEDI
                     IWNNISFTKGIEKKSTKWTYMVKRSLCKLNDGDGQFLESVLLNQIEKPTLYPLGEAEQ
                     KKLKTLTVKTQDSKQVTVSIPENEDVPTISVNQQQSTQRDSIKIQALLAEIGERMNFK
                     IWVPRSDRQRVLEVWHPKTQCLLEQLPLNYDDATLKTIENIDVLWIKGRSIVRAFEVE
                     HTTSIYSGILRMADLMALQPNLNIRAHIVAPAERKEKVLQEISRPVFAFLEKGPLSES
                     CTFISYESIQELSNEKSLEYMNDNVLEKFEEYAEEADI"
     gene            1797..2900
                     /gene="meaB"
                     /locus_tag="PLW22_07475"
     CDS             1797..2900
                     /gene="meaB"
                     /locus_tag="PLW22_07475"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOU74582.1"
                     /translation="MEQKKKEEKSALIVNPGIEQPPMVNEDAIRRFKAKKRKLLSIEE
                     YMDGIKNGNIPILSQAITLIESSLQQHQELARELVEQCLPLSGRSVRIGITGVPGVGK
                     STFIEALGSTITAQGHRLAVLAVDPSSERSKGSILGDKTRMETLSADPNAFIRPSPSA
                     GSLGGVARKTRETIILCEAAGFDTIFIETVGVGQSEVAVHSMVDFFLLLMLAGAGDEL
                     QGIKRGIMEMADMIAITKADGSNIQKAKLAQAEYQNALHLFPATESGWVPQAVTCSSV
                     NKTGILEIWNTINQYMAFTKANGYFSHRRSQQAKYWMYETINEELKNRFYHSTQVSTM
                     IKWIEHEVTHNKLSSFAAAQKLIDVYFEQNGNK"
     gene            2907..3491
                     /gene="thiF"
                     /locus_tag="PLW22_07480"
     CDS             2907..3491
                     /gene="thiF"
                     /locus_tag="PLW22_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOU74583.1"
                     /translation="MTFDEIKQRLKHYTVGIAGAGGLGSNVAMHLTRVGIGKLIVADF
                     DVVQESNLNRQFYFRHQLGAKKVEALKTNLLAINPELNIVIHSIKLDALSIREIFNDV
                     DIMVEAFDRAEMKEVIAEVFQTEYPNIPLVMGNGMAGWGKSNEMRVNRFGNLYICGDG
                     VSEIADELPPLSPRVGICAAMQANVVLELLLGSK"
     gene            3606..4229
                     /locus_tag="PLW22_07485"
     CDS             3606..4229
                     /locus_tag="PLW22_07485"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOU74584.1"
                     /translation="MFSGIVEEPAKVVALEREKENLHITLTCSFVNDLKIDQSVSHNG
                     VCLTVVKKDNSTYTVTAIKETLEKSNLGQLKVGDKVNVERSMKIDSLLDGHMVQGHVD
                     QTAVCTDVQEADGSWYYTFEYDPSKGNITVEKGSIAVNGVSLTVVNSRENSFQVAIIP
                     YTYEHTNFHEIKKGTVVNLEFDIVGKYITKILKQYLESGQLNRLTGK"
     gene            4235..5293
                     /locus_tag="PLW22_07490"
     CDS             4235..5293
                     /locus_tag="PLW22_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU74585.1"
                     /translation="MTVKKIFESPRSLSLTIASIVSVLAAIFFLFIVNFTQGQQLLKL
                     VAFTLSVFTITFLSSLLLVNLFVSKRIKPLYKALYSIDKANADLVDELDQNNLLQRLE
                     YDLRVWAEAKTDEIDRLKQMERYRKEFLGNVSHELKTPIFNIQGYVLTLLDGGLEDPS
                     INRRYLERTEKSINRLIGIVEDLELITKLEAGELQLNLERFNIISLIEETFEALEQRA
                     KHKSITLELDKEYSKPIWVYADKKRISQVLYNLIINSIFYGKENGVTTVSVSDIGTRV
                     QVEVNDNGIGIDQKDLPRIFERFYRVDKSRSRDQGGTGLGLAIVKHIIEGHKQVIGVR
                     SSLNQGTTFSFTLDKPKK"
     gene            complement(5312..6187)
                     /locus_tag="PLW22_07495"
     CDS             complement(5312..6187)
                     /locus_tag="PLW22_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74586.1"
                     /translation="MRLKVTPLVVLLLVLFLVNAEAQNRPPQNVRRVIVTSRYEVKDG
                     KRTINSRAIKQEIKDSLDRVHTILFRDYETQGVYRYIWHTYEGEQIVRTDEFANEKLF
                     RYSLFTYTPDSLVATQKVYLVNPGDTVHYVTLSFKYQNRLLIQVDAHNSKGKRVYRTK
                     SVYDNYGTEILRQVKVSKVYIPVDSIVNLSCKPTYDTLGRKVAETITKTFADGRKEAK
                     SFKYEYNKKNLRSAVEEFDSAGKLLSRTTLEYNDKGMLKFISVFNSEGQLIDYQALRY
                     ELYPSLDRRKQIIEY"
     gene            6311..7129
                     /locus_tag="PLW22_07500"
     CDS             6311..7129
                     /locus_tag="PLW22_07500"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOU74587.1"
                     /translation="MHEDLRQLVEQAWDNRELLNQKEYIQGIEAVIDLLDKGQIRVAQ
                     PIEKGWLVNEWVKKAVLMYFPIRKLEVMEAGILQFYDKIPLKRDFSAIGVRAVPHAVA
                     RYGSYLAKGVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKGVHLSGGVGIGGVLE
                     PVQAAPVIVEDGCFIGSRCIIVEGVHIGREAVLGANVVITQSTKIIDVSGPEPVEYRG
                     FVPERSVVIPGSYTKKFPAGDYQVSCALIIGKRKESTDLKTSLNNALRDFGVSV"
     gene            7402..8247
                     /locus_tag="PLW22_07505"
     CDS             7402..8247
                     /locus_tag="PLW22_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU74588.1"
                     /translation="MADIDFLKKMAAQVRRDVIRMVHKPASGHPGGSLGCADFFTALY
                     FEVMNIDPKNFTMDGTGEDVFFLSNGHLSAAWYSVLARRGFFEVAELSTFRLINSRLQ
                     GHPTTAEHLPGIRVASGSLGQGLSVACGAALAKKLNGDKNLVYTLHGDGELQEGQIWE
                     AALFAAAKKIDNLIATVDYNGKQIDGPVDEVVTLGNLKAKWESFGWEVLESNGNDMEK
                     LISTFALAKSKTGQGKPIVILMRTEMGMGVDFMMGTHKWHGKAPNDEEFARAMAQLPE
                     TIGDF"
     gene            8281..8682
                     /locus_tag="PLW22_07510"
     CDS             8281..8682
                     /locus_tag="PLW22_07510"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOU74589.1"
                     /translation="MQNIALIAHDAKKPELVRFLKEHEDWLPGVNLLATGRTAEFLEE
                     QGIKCRHLSPGKSGGYNEITEMIGKGEIDIVIFLRDHKVVQPHHEDIRRLLEACNVNN
                     IPLATNKATAELVILGLIRKESIERMKRNID"
     gene            8783..9733
                     /locus_tag="PLW22_07515"
     CDS             8783..9733
                     /locus_tag="PLW22_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOU74590.1"
                     /translation="MKYTSTGNKDTRSGFGAGLHELGRTNPNVVALCADLIGSLKMDD
                     FIKENPNRFFQVGIAEANMIGLAAGLTVGGKIPFAGTFAAFATGRVYDQIRQVVAYSN
                     KNVKICASHAGLTLGEDGATHQMLEDIGMMKMLPNMTVIVPCDYNQTKAATLAIADHV
                     GPVYLRFGRPAVPNFTPADQKFEIGKAQLLVEGSHVTIFATGHMVWKALEAADILEQK
                     GISAEVINIHTIKPLDEEAVLKSVSKTRCVVTAEEHMMNGGLGDSIAQLLARRMPTPV
                     EMVAVNDTFGESGKPEELLVKYGLDTPNIVEAAQKVLARM"
     gene            9749..10297
                     /locus_tag="PLW22_07520"
     CDS             9749..10297
                     /locus_tag="PLW22_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU74591.1"
                     /translation="MDNHTDSELLVMFRNPETKHYAFNLLVRKYQERLYWQVRKIVIS
                     HDDADDVIQNTFLKVWGGLENFREDSQLFTWLYRIATNEALTFLKKKRTKLLLPIVDV
                     EQQLARTLEGDPFIDGDSTALKLQKALLKLPEKQRLVFNMKYFEEMKYEEISEILGTS
                     VGALKASFHHAVKKIEKFLEED"
     gene            10368..10805
                     /locus_tag="PLW22_07525"
     CDS             10368..10805
                     /locus_tag="PLW22_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74592.1"
                     /translation="MKSMEEKFNKKLPFKVPEGYFDNLPLRVQERIAATETQHEPKSR
                     WVTLKSQLAFAAGFAFMALLSYLGYYMARPLMADKKAVKTDYVEIVSRTIYEFDDIDL
                     YKAIEQKKKTDSLKEVYNEIPHRLYVRSSNCITIIDEKKEIKP"
     gene            10802..11245
                     /locus_tag="PLW22_07530"
     CDS             10802..11245
                     /locus_tag="PLW22_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74593.1"
                     /translation="MRQLFRILIVFIFIALGAKAYSQTDDTEKALKAEKVAFLTKKLD
                     LTQDEAKKFWPLYDEYWEKKIKILNDRRKLADDFIQNINNITDKEAVDYARRYVESQK
                     RESDLIAEYNKLLLQILPPKKVMLLYQSNYEFKNYLLQKVQETKK"
     gene            11332..11721
                     /locus_tag="PLW22_07535"
     CDS             11332..11721
                     /locus_tag="PLW22_07535"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HOU74594.1"
                     /translation="MTELVDLFIPATTETPEIKCEKSSGRIMMVGNLIPSDPQSFLAP
                     IHRWLQSYVTTYTPQNITVEFYLTFINGCSEHHLGKLLKYLEELHINGMSVKVICHYE
                     NEDLDMKEWGKDLSMALRIPIELVEIN"
     gene            11822..12244
                     /locus_tag="PLW22_07540"
     CDS             11822..12244
                     /locus_tag="PLW22_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOU74595.1"
                     /translation="MSINEIQDAIVEEFSVFDDWMDRYQQLIEYGKDLPPIDERKRTE
                     QYLIQGCQSKVWLDAELRDGKIYFTADSDAIITKGIVALLIKVLSGRTPDEILNAELY
                     FIDKIGLKENLSPTRSNGLVAMVKQMRMYALAFKAKAE"
     gene            12294..12608
                     /locus_tag="PLW22_07545"
     CDS             12294..12608
                     /locus_tag="PLW22_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HOU74596.1"
                     /translation="MRDELEIQTDIVKTLKGIHDPEIPVNIYDLGLIYEVDVDDNRKV
                     TITMTLTAPNCPLADQVVEEVQEKISKIDGVTKVEVRLTFDPPWDKSRMSDEAMLELG
                     FL"
     gene            12623..13534
                     /locus_tag="PLW22_07550"
     CDS             12623..13534
                     /locus_tag="PLW22_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU74597.1"
                     /translation="MEKKSQSRAYFLASFVVLFWGTSATAFKIGLSHTEPVQLLFWSS
                     LSASIILFVVLTFQKKLPLLKFTSKKEILYSLAESVLNPFLYYLVLFKAYSLLPAQVA
                     QPLNYIWPIVLVVLAALLLKHPLRFTDIIALLFSLIGVAFISSQGNINIFAKSNPLGV
                     LLAVGSSVIWASFWIVNMYDKHRPEEVKLFISFALATVLTLLYMAAIGDLPVFNLKPL
                     AASVYIGAFEMGITFVLWLKALNSATNTARLGNFSYLVPFVALVFVSLVLREKIVWTT
                     LVGLIVIIGAIVFQQLFSKKDIKDEPI"
     gene            13521..14774
                     /locus_tag="PLW22_07555"
     CDS             13521..14774
                     /locus_tag="PLW22_07555"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU74598.1"
                     /translation="MNRFKLAILLLLGLAAFTSCEDDEQKVNVNKEVYNLMKDWYYWY
                     DQLPNVDPNSYPDPVELVKAIRVNPPDRWSYVTTKQELDAYYKQAVYIGFGFGSAFTA
                     DNKLIITFVFKTSPLYANGIGRGWEIVSIDGQTPTPDNYTSLIGPSEVGVTKTFVFKS
                     PTGQTTQHTFSKAQIEMNTVLMDSVYTINGKKVGYFVLKGFIELTATELETVFQKFKN
                     AGVSELICDLRYNGGGQVNVSQLLGNLIGGSIAFGRVFGQYIHNNKHSDKNSYLYFST
                     NQYSLEIQRAVFITTSSTASASELVINALKPHMDVVLVGSKTHGKPVGMYTFQFNDPS
                     IDWAIVPVCFSIRNSNNEGDYYDGIPVTVEAADDIFTPFGEVTESSLNSALNYLGLGT
                     KAVTKSYVTAQPITGKGLYEEIGAW"
BASE COUNT         4485 a         3017 c         3306 g         4003 t
ORIGIN      
        1 gaagaataga attgaattaa ctgattttat gatacttgaa ataaatgagt ataataaaaa
       61 atacttatca gtttttaatc aagggattaa ctctaataaa ttaccaagag aaccgaaaat
      121 aaaaatcgat aagaaaatga ttaacaactt tttaatatac agttcaaagt tctaaaacat
      181 agaacataac aagtgttgca ttgcatgtta ggaatctgct agatatgatt tttttgcttc
      241 aaaccatttt tcttaacatt gtacgacttg gttcaaaaca ccagcccgca aacgtttcgg
      301 tgcaaccgaa aaagacaaaa aacattaaca tatggcatat tacattaact tattctcacc
      361 cgacacgtat caaaagtttt cggactctga taaaagtatt tcaggagttt cagacaaact
      421 aaaagatgtt gcttcaacta tcaaaccagg cgataaacta atttgttatg tgacaaaatt
      481 atcacgctgg gttggagttt tagaggtaac gagtaactat tttataaatg actcaccaat
      541 tttcacccaa acggccgacc cttatgttgt aagatttaat gtaaaaccga taatctggct
      601 accattggaa aatgctatac cagttcatga agatataatt tggaataata tatcatttac
      661 taagggtata gaaaaaaaga gtaccaaatg gacttacatg gttaaaagaa gtttgtgtaa
      721 actgaatgac ggagacgggc aattccttga aagtgtttta ttaaatcaaa tagaaaaacc
      781 tactctatat cccttaggtg aagctgagca aaaaaagtta aagacgctga cagtaaagac
      841 acaagacagt aaacaggtaa ccgtttcaat acccgaaaac gaagatgttc ccactatatc
      901 cgtaaatcaa caacaatcaa cccaacgtga ttccattaaa atacaagcac tacttgcgga
      961 aattggcgaa cggatgaatt ttaaaatttg ggtaccacgt agtgacagac aaagagtttt
     1021 ggaagtttgg catcctaaaa cacaatgttt acttgagcaa cttccgttaa attatgacga
     1081 tgccactttg aaaacgattg aaaacattga tgttttgtgg attaaagggc gttcaattgt
     1141 tcgtgctttt gaagttgaac atacaacatc aatttattca ggaattttaa gaatggctga
     1201 cctaatggcc ttacagccaa atttaaatat tagggctcac attgtggctc ctgccgaaag
     1261 aaaagaaaaa gtcttacaag aaatatcaag gccagttttt gcatttttag aaaagggtcc
     1321 tttatctgaa tcgtgtacat ttatatctta tgagtcaatt caagaacttt ccaatgaaaa
     1381 gagtttagaa tatatgaatg acaatgttct tgaaaaattt gaggaatatg ctgaagaagc
     1441 agacatatag acaaaaacgg aagcctttaa taaaactgta atttttaatc ctgttaaaat
     1501 tacattcatg cttatttgag cagaaccgat gctaaatcgc acaacttcat atttcttagc
     1561 catttgggca gattgcttat catgacaatg tttggaagtt aatttaatcg ctacattatt
     1621 gcaatcatct tgtaaccaac agccctcatc tacttcaacc gcaacgagct ggtagcttaa
     1681 tgaaccaagg atgttcgtaa acaaaaagaa attgtgcctc tttttaagta aacccattca
     1741 cccaattagg ttttacagaa tagaatgaat aaattcgcgt atccaagcga tacaagatgg
     1801 aacagaaaaa gaaagaggag aaaagtgcac ttatagtaaa tccgggcatt gaacagccac
     1861 ccatggtgaa tgaggatgcc attaggcgat tcaaggcaaa aaagcgcaag ctcctgagca
     1921 ttgaggagta catggatggt atcaagaatg gcaatattcc catactcagc caggcaatta
     1981 cccttattga gagttcgctt cagcaacatc aggagttggc aagggaactg gttgagcaat
     2041 gcctccccct ttcgggccgt tcggttagga ttggtataac cggtgttccc ggagttggta
     2101 aaagcacttt cattgaggcg ctgggaagca ccataaccgc tcagggacac aggctagccg
     2161 tgctggcagt tgacccaagc agcgagcgga gcaagggaag catacttggc gataaaaccc
     2221 gcatggaaac ccttagcgcc gatcccaatg cattcatcag gccttcgccc tcggcgggtt
     2281 cactgggcgg ggttgcccgc aaaacgcgcg agaccattat tctttgtgaa gctgccggtt
     2341 tcgatacaat atttattgaa acggttggtg ttgggcaaag cgaggtggcg gttcattcca
     2401 tggtcgattt cttcctgctg cttatgcttg ccggcgccgg tgatgaactt cagggaatta
     2461 agcgcggcat aatggaaatg gccgatatga ttgccattac caaagccgat ggaagcaaca
     2521 tccaaaaggc aaagcttgcc caggccgagt accagaacgc cttacacctt ttccctgcca
     2581 ctgaatcggg atgggtgccc caagcggtta catgctcatc ggttaacaaa acaggtatat
     2641 tggagatttg gaataccata aaccagtaca tggcttttac caaggcaaat ggctacttta
     2701 gccacaggcg aagccagcag gcaaagtact ggatgtacga aaccattaac gaggaactga
     2761 aaaaccgttt ttaccacagc acacaggtga gtaccatgat taaatggatt gagcacgagg
     2821 ttactcataa caaacttagc tcatttgcag ccgcacaaaa actaatcgat gtgtattttg
     2881 aacagaatgg caataagtag aattgtatga cctttgatga gataaaacaa cgcttaaagc
     2941 actacacggt tggcattgcc ggtgcaggtg gacttggcag taatgtagcc atgcacctca
     3001 cgcgggttgg cattggaaag ctgatagttg ccgactttga tgtggttcag gaaagcaacc
     3061 tgaacaggca gttctacttc aggcaccagc ttggcgccaa aaaggttgag gcgctaaaaa
     3121 caaacctttt agccataaac ccggaactta acatagtaat acacagcatt aagctcgatg
     3181 ccctaagcat cagggaaatt tttaatgatg ttgatattat ggttgaggcc tttgacaggg
     3241 ccgaaatgaa agaggtaatt gccgaggttt tccaaaccga ataccccaac attcccctgg
     3301 taatgggcaa tggtatggcc ggttggggta aatcgaacga aatgcgggtt aaccggtttg
     3361 gcaaccttta catctgcggc gatggagtat cggagattgc cgatgagctg cctcccctct
     3421 cgcccagggt gggtatctgt gcagccatgc aggccaatgt ggttttggaa ttgctgttgg
     3481 gtagcaagta gtttaactca agtcctacag cttcataaag aagcgatagc cttttaatac
     3541 taaatctgtt tgaagcaaaa tcaatttata ctatatcttt gcatcaaaag taaaaatcaa
     3601 actttatgtt ttcaggaata gttgaggaac cggcaaaagt ggttgcgttg gagcgggaaa
     3661 aggagaacct acatatcact ctaacctgct cgtttgtaaa tgatttaaag attgaccaga
     3721 gcgtttcgca caacggcgtt tgtttaactg tagtgaaaaa ggacaactct acttacactg
     3781 ttaccgccat taaggaaacc cttgagaaat cgaacctagg gcaactcaag gtaggcgata
     3841 aggttaacgt ggaacgcagc atgaaaatcg atagcctgct cgatgggcat atggtacaag
     3901 gccatgttga ccaaactgcc gtttgcaccg atgttcagga agccgatggt agctggtact
     3961 acacttttga gtacgacccc tccaagggaa atattaccgt tgaaaagggt tcaattgccg
     4021 taaacggcgt aagcttaacg gtggttaact cccgtgagaa ctcgtttcag gtggctataa
     4081 tcccatatac atacgagcat accaactttc atgaaattaa gaaaggaacc gttgtaaacc
     4141 tggagttcga tattgttggt aaatacatca ccaaaatact taagcagtac ctggaatcgg
     4201 gccagcttaa caggcttacc ggcaagtaaa acacatgacg gtaaaaaaaa tctttgaatc
     4261 gccacgttcc ctctcgttaa ctattgcatc tatagttagc gtactggctg caatattttt
     4321 tctgtttatc gtcaacttta cgcaggggca acagctgctt aagcttgttg cttttacgct
     4381 ttctgttttt acaataacct ttctgagtag ccttttactg gtcaacctat ttgtgtcaaa
     4441 aaggattaag cctctataca aggctttata cagcattgat aaggccaacg ccgaccttgt
     4501 tgacgagctt gaccagaaca acctgctaca gcggctcgag tacgacttaa gggtatgggc
     4561 cgaagcaaaa actgacgaaa tagaccggtt aaagcagatg gagcgttacc gtaaggagtt
     4621 cctgggaaac gtatcgcacg agcttaaaac ccccattttt aatattcagg gatatgtgct
     4681 caccctgctg gatggcggac tggaggaccc aagtattaat cggcgttacc tggaacgtac
     4741 cgagaaaagc ataaaccgct taattggtat tgttgaggat ctggagctaa tcacaaagct
     4801 tgaggctggt gaactgcagc tcaacctgga acgcttcaac attattagcc tgattgagga
     4861 aacatttgag gctttagagc aacgggcaaa acataaatca atcacccttg agctcgataa
     4921 agaatacagc aaacccatct gggtttatgc cgataaaaaa agaatctcgc aggtacttta
     4981 taatcttatt atcaactcca ttttttatgg gaaggagaac ggggtaacca ctgtatcggt
     5041 ttccgatatt ggaacaaggg tacaggttga agtaaatgat aatgggattg ggattgacca
     5101 gaaggattta ccccgaatat ttgaacgctt ttacagggtt gataaaagcc gttcgcgcga
     5161 ccagggtgga accggacttg gccttgccat tgtaaagcat atcattgaag ggcacaagca
     5221 ggttattggt gtacgcagta gcctaaacca gggaacaacc tttagcttta cgctcgataa
     5281 acctaaaaaa taggcagtac caagtttggt tttagtactc aatgatttgt ttccgcctat
     5341 cgagcgaggg gtaaagctcg tatcgcaaag cctgatagtc gattagttga ccttctgagt
     5401 taaaaaccga tataaactta agcatgccct tatcgttgta ctcaagggtt gttcggctta
     5461 gtagtttccc cgcagaatcg aactcttcca ccgctgagcg caggtttttc ttgttatact
     5521 cgtatttaaa gcttttagct tccttacggc cgtcggcaaa ggtttttgta atagtttcag
     5581 ccaccttcct gcccagggtg tcgtaagtgg gtttacaact caaattcact atggagtcaa
     5641 ccggaatata gacctttgat actttaacct gacgtagtat ttcggttccg tagttgtcgt
     5701 aaaccgactt ggttcggtaa acccgcttcc ccttgctgtt gtgggcatca acctgaataa
     5761 gtagcctgtt ctggtactta aagctaagtg taacgtaatg aacggtatcg ccagggttta
     5821 ccaggtaaac cttttgggta gctactagcg aatcgggtgt ataggtgaaa aggctgtacc
     5881 tgaaaagctt ttcgttagca aattcatcgg ttctaacaat ttgttccccc tcataggtgt
     5941 gccaaatgta gcgatacacc ccctgggttt cataatcgcg gaatagaatt gtatggactc
     6001 tatcgagtga atccttaatc tcctgcttaa tggcccggct attaattgtt cgttttccgt
     6061 cctttacctc atagcggctg gttactatca cacgccttac attctgtggt ggacggtttt
     6121 gagcctcggc attcactagg aaaagcacca gtaacagaac aactaaaggg gtaactttta
     6181 gcctcatagc aatcagtttt tatgttgcaa tttaacgaaa ttttccctga aaggttttac
     6241 tctaaaactg ttttgaaaag tataagcagt aaactaccta tttgttacat ttgcaaaaat
     6301 gtttgagtag atgcatgaag atttaagaca acttgttgag caagcctggg ataacaggga
     6361 acttcttaac cagaaagagt acattcaggg tatcgaggct gttattgact tactcgataa
     6421 ggggcaaatc cgtgtagcac aacccattga aaagggttgg ctggtaaatg aatgggtcaa
     6481 gaaagcggta ctgatgtact ttcccatacg gaagcttgag gttatggagg cagggattct
     6541 gcaattctac gataaaatac cccttaaaag ggattttagt gcgattggtg tacgcgcggt
     6601 gccgcatgcc gtagcccgct atggcagtta ccttgccaag ggtgtggtgc tgatgccctc
     6661 gtatgttaac attggggcat atgttgatga aggaaccatg gtggatacat gggccaccgt
     6721 gggctcctgc gcacaaattg ggaaaggtgt ccacctgagc ggtggcgttg gaattggcgg
     6781 ggttcttgaa cccgttcagg ctgcaccggt aatagttgag gatggttgct ttattggttc
     6841 gcgttgtatc atagttgagg gggtgcacat tggccgggaa gctgtacttg gcgccaatgt
     6901 tgtgataact caatcaacaa aaattattga tgtgtcgggc cctgagcctg ttgaataccg
     6961 cggatttgtt ccggaacgct cagtggttat accgggatca tacacaaaga aattccccgc
     7021 cggcgattat caggtttcgt gcgcattaat tattggtaaa cgcaaggaat ctactgacct
     7081 aaaaacctcg ctcaacaacg ccctcaggga ttttggggtg agtgtttaac gcaagtactg
     7141 tgagtataga gtaatgaagc ggaatgtgta ggaatagtat aatttgattg aaattgagta
     7201 cgttaattgt aactgggatg ttaaattttt aaggaaattc gtaaatgaaa aaagttgttt
     7261 agatgttttc agtatttcca ttaatcaatc tcagtttaac aaatcttaaa tgtactaatt
     7321 ccgccttacc tgaaagcagt atcttttaag tgaagaaaag tattaccttt gtcggttaat
     7381 tatcatgtaa atacagtttt gatggcagat attgattttc ttaagaaaat ggcggcacag
     7441 gttcgccgcg atgttatccg tatggttcat aaacctgcaa gcggtcaccc cggaggttcg
     7501 cttggctgcg ccgatttctt taccgcactc tactttgagg tgatgaacat cgatccaaaa
     7561 aactttacca tggatggtac tggtgaggat gttttctttt tgtcgaatgg tcacctatcg
     7621 gctgcgtggt acagcgtgct tgcccgccgt ggattttttg aggttgcaga gctatcaaca
     7681 ttccgattaa tcaactcacg tttacagggc caccccacca ctgctgaaca cttacctgga
     7741 attcgcgtgg cttcgggatc gttgggacaa ggcctttcgg ttgcatgtgg tgctgctctg
     7801 gcaaaaaagc ttaatggcga taaaaatctg gtttacaccc tgcacggcga tggcgagctg
     7861 caggagggtc agatttggga agcagccctt tttgccgctg ccaaaaagat tgataacctt
     7921 attgctactg ttgactacaa cggaaaacaa attgacggcc cggttgatga ggtggttacc
     7981 cttggcaacc taaaggctaa atgggaatcg tttggctggg aggtgctgga atccaatggc
     8041 aacgacatgg agaaactcat ttccaccttt gctctcgcta aaagcaaaac cggccaaggt
     8101 aagcccattg tcatactgat gcgaaccgaa atgggtatgg gggttgattt tatgatggga
     8161 acgcacaaat ggcatggcaa ggcccccaac gacgaagagt ttgcccgtgc tatggcacaa
     8221 ctccctgaaa caattggcga tttttaggct ttagttataa aaaataaaaa aagcaaagct
     8281 atgcagaaca ttgccctcat tgcgcacgat gccaaaaaac ctgaacttgt caggttcctg
     8341 aaagagcatg aagactggtt accaggtgta aaccttttgg caaccggtag aactgctgag
     8401 ttcctggagg aacagggcat taaatgccgt caccttagcc cggggaaatc gggcgggtat
     8461 aatgagatta cggaaatgat tggtaaaggt gaaattgata tagtgatatt cttgcgcgac
     8521 cataaggtgg tgcaacccca ccacgaggat atccgccgtt tgctggaagc atgcaatgtg
     8581 aataacattc cgcttgctac caacaaagct acagctgaac tggttatcct tggccttatc
     8641 cgtaaggaat ccattgagcg aatgaaacga aatatcgact aaagaacttt tcactttacc
     8701 agaatcaggt taaccctacc tactagggta atggctttta atcatttttt tacagttaca
     8761 acgcaaaagc aaaaaataaa taatgaaata tacttcgact ggaaataagg acacccgttc
     8821 cggatttgga gccggactac atgagctggg acgcactaac cccaatgtgg tagcgctatg
     8881 tgccgatctt attggatcgc ttaaaatgga cgattttata aaggagaatc ccaatcgttt
     8941 ctttcaggtt ggaattgctg aggccaacat gattggactt gcagccggat taaccgttgg
     9001 tggcaaaatt ccatttgccg gcacctttgc cgcttttgca accggtaggg tttacgatca
     9061 aattcgccag gttgtagcct actccaacaa aaacgttaag atttgcgctt cgcacgccgg
     9121 ccttacgctg ggcgaggacg gagcaacgca ccaaatgctg gaggatattg ggatgatgaa
     9181 aatgcttccc aatatgaccg ttattgtccc ctgcgactat aaccaaacca aagccgcaac
     9241 gctggccatt gctgaccatg tggggccggt ttaccttcgc tttgggcgtc cggcagtacc
     9301 caacttcacc ccagccgacc agaagtttga aattggcaag gctcagctcc tggttgaggg
     9361 tagccatgtt accatatttg ccaccggcca tatggtttgg aaagcccttg aggcagccga
     9421 tatacttgag caaaagggta tctcagccga ggttattaac atccacacca ttaagccgct
     9481 cgatgaggaa gcagttctga aatccgtttc caaaacacgc tgtgtggtaa ctgccgagga
     9541 gcacatgatg aatggaggtc ttggcgatag cattgcacag cttttagcac gcagaatgcc
     9601 cacaccggtt gaaatggttg ccgttaacga tacctttggg gagagtggca aacctgaaga
     9661 actactggtg aaatacgggc tcgatactcc taatatcgtt gaagctgctc aaaaggtatt
     9721 ggcacgcatg taaaataacc atttacgaat ggataaccat accgatagcg agttactcgt
     9781 aatgtttcgg aatcccgaaa cgaaacatta cgcttttaac ttgctggtta ggaaatatca
     9841 ggaacgacta tactggcaag tccgcaaaat tgttattagc cacgacgatg ccgacgatgt
     9901 tattcaaaat actttcctta aggtatgggg cgggctggag aactttcggg aagattccca
     9961 gctctttacc tggttatacc gaattgccac caacgaggca ttaacgtttc tgaagaagaa
    10021 acgtacaaaa ttgctcctcc ccattgttga tgtggaacag cagcttgctc gaacccttga
    10081 gggcgatccc tttattgatg gcgacagcac agcacttaag ttacagaagg cactccttaa
    10141 actacctgag aaacagcgct tggtctttaa tatgaagtac tttgaggaga tgaagtacga
    10201 agaaatttct gaaatccttg gcacctccgt tggtgcgctt aaggcttcgt tccatcatgc
    10261 tgttaaaaaa attgaaaaat ttttagagga ggattaaacc tatacctaaa catactatca
    10321 tagttttaga tagatatagc aatagcgcat ttggaaggag aatgattatg aaaagcatgg
    10381 aggaaaaatt caacaaaaag ttacccttta aggtaccgga aggctacttt gataacctac
    10441 ctctaagggt acaggagcgc attgcagcta ccgaaacaca gcatgaacca aaaagccgat
    10501 gggtaaccct taaatcgcag ctggcatttg ctgctggttt tgctttcatg gctttgctaa
    10561 gctatttggg ttattacatg gctcgcccac ttatggctga taaaaaagcc gttaaaaccg
    10621 attacgttga aattgtaagc cgaaccattt atgagttcga cgatattgat ctttacaagg
    10681 ccattgaaca gaaaaagaaa accgatagcc ttaaagaggt ttacaacgaa attccccaca
    10741 ggctatacgt taggagcagc aactgcataa caattattga cgaaaagaaa gaaattaaac
    10801 catgaggcaa ctctttagga tactaatcgt ttttatattt atagccctgg gggcaaaggc
    10861 ttactcacaa accgacgata ccgagaaagc ccttaaggcc gaaaaggttg cttttttgac
    10921 caaaaagctc gatttaaccc aggatgaagc caaaaagttt tggcctttat acgatgagta
    10981 ctgggaaaaa aagattaaaa tacttaacga ccgtcgcaag cttgccgatg actttatcca
    11041 aaacataaac aacataaccg ataaggaagc tgttgattat gccagaaggt acgtggagtc
    11101 ccaaaaaagg gaatcggatc tgatagcaga gtataacaag ctactccttc aaatactccc
    11161 acccaagaag gttatgcttc tgtaccaatc caactacgag ttcaagaatt acctgcttca
    11221 aaaagttcag gaaacaaaaa aatagttata aacatcataa gtttttaact tttttttaca
    11281 tttgtcgggt tacatgttaa ttcttttgag gtaatggtaa acgaggagaa agtgactgaa
    11341 ttagttgatt tgtttattcc ggcaacaact gaaacacctg aaattaaatg tgaaaagagt
    11401 tcagggcgaa taatgatggt gggcaacctg atccctagcg atccccaatc atttctcgca
    11461 ccaatacatc gttggctaca gagctacgtt acaacctaca ccccacaaaa cataaccgtt
    11521 gagttctacc taacctttat aaacggatgc tctgagcatc accttgggaa gttactgaaa
    11581 taccttgagg agcttcacat caacgggatg tccgttaagg ttatttgcca ctacgagaat
    11641 gaggatttgg acatgaagga atggggaaaa gatctaagca tggcactccg aatccccatt
    11701 gagctggttg aaatcaacta agaagtcagc attagtataa tacgttacag caaagctcaa
    11761 ccgctttgct gtttttcgtt ccaatgcgga aactgcttat atttgctaaa aaaatagaaa
    11821 aatgtcaatt aacgagattc aggacgcaat agttgaggaa ttcagcgttt tcgacgactg
    11881 gatggatcgc taccaacagc tcatagagta tggcaaggac ttgccgccta tagatgagcg
    11941 caagcggaca gaacagtacc ttatacaggg ctgccaaagc aaggtttggc tcgatgctga
    12001 gctgcgcgat ggaaaaatct actttactgc cgatagcgat gccataatca ccaaaggaat
    12061 tgttgcgctg ctaataaaag ttctctcggg ccgcaccccc gatgagattc ttaatgctga
    12121 gctttacttt atcgacaaaa ttggcttaaa ggaaaaccta tcgcctaccc gtagcaacgg
    12181 actagttgca atggtaaagc aaatgcgtat gtatgccctt gcctttaagg caaaagcgga
    12241 gtgaaccggt taaaccttat gaaacaaaag ctgtttctta taaaaaaata gatatgcgcg
    12301 acgaactcga aattcaaacc gatattgtca aaacactcaa gggcattcac gaccctgaaa
    12361 taccggttaa catttacgat ttagggttaa tttacgaggt tgatgttgac gataatcgta
    12421 aggttaccat aaccatgact cttaccgctc ctaactgccc gcttgccgat caggtagttg
    12481 aagaggttca ggaaaagatt tcaaaaattg atggggtaac caaggtagag gtacgcttaa
    12541 cctttgaccc tccctgggat aaaagccgta tgagcgacga agctatgctg gaacttggct
    12601 ttctgtaacc atcggtataa aaatggaaaa gaaaagccaa agccgtgctt actttctggc
    12661 atcgtttgta gttctctttt ggggcacatc ggccacagct tttaaaatcg ggttaagtca
    12721 tacagaaccg gttcaactgc ttttttggtc atcgctttcg gccagtataa tactgtttgt
    12781 tgttttaaca tttcaaaaaa aactaccgct attaaaattc actagcaaaa aggagatact
    12841 ttactcgctt gccgaatcgg ttttaaatcc tttcctatac taccttgtgc tttttaaggc
    12901 ctacagcctt ttacctgctc aggttgctca acccctaaat tacatttggc caatagttct
    12961 ggttgttttg gcggcattac tactaaagca ccctcttagg tttactgata taattgcatt
    13021 actattcagc ttgattggcg ttgcgttcat atcgtcgcaa ggcaatatca atatttttgc
    13081 aaaatctaat ccgctgggag ttctactggc agtagggagt tcggtaattt gggctagctt
    13141 ttggattgtc aacatgtacg ataagcatcg ccccgaagag gttaaactgt ttataagttt
    13201 tgctttagct acggttctca ccctgcttta catggctgca ataggggatt taccagtttt
    13261 taatctgaaa cctttagccg catcggttta cattggagca tttgaaatgg gcataacgtt
    13321 tgtgctttgg ctaaaagcat taaatagcgc aacaaacaca gcccgtttag gcaatttttc
    13381 gtatcttgta ccgttcgttg ctttagtatt tgtgagcctg gtgctccgcg aaaagatagt
    13441 atggactact ctagtaggac ttatagttat tattggtgcc attgtatttc aacaactttt
    13501 ttctaaaaag gatattaaag atgaaccgat ttaaactagc aatcctcctt ttattaggat
    13561 tagcagcatt tacctcgtgc gaggatgacg agcaaaaagt aaatgttaat aaagaggttt
    13621 acaacctcat gaaggattgg tactactggt acgaccagtt gcccaatgtt gatcccaaca
    13681 gctatcctga ccccgtggag ctggtaaaag ccattcgggt taaccctccc gaccgttgga
    13741 gctacgttac cactaagcag gaacttgatg cctactataa acaagccgta tatataggct
    13801 ttggatttgg tagtgcattc acagctgaca ataagctaat tattacattt gtgttcaaaa
    13861 catcgccact ttatgccaat ggtattggta gaggatggga aattgttagt atcgatgggc
    13921 aaaccccaac ccccgataac tacacctccc taattggccc atcggaggtt ggtgttacta
    13981 aaacctttgt gttcaaatcg cccacagggc aaacgactca gcatactttt agtaaagccc
    14041 aaattgaaat gaataccgtg ctgatggata gcgtttacac cattaacggg aaaaaggttg
    14101 gctactttgt tctgaaaggg tttattgaat taactgccac tgagcttgaa acagtatttc
    14161 agaaatttaa aaatgccggc gtaagcgagc taatctgtga cctgcgctat aatggcggcg
    14221 gccaggtaaa tgtttcgcag ctattaggaa atcttattgg tggtagtatt gcatttggta
    14281 gggtttttgg gcagtacatt cataataaca aacattccga caagaatagt tacctgtact
    14341 tttcaaccaa tcagtactcc ttagaaattc aacgtgcggt attcattact accagcagca
    14401 cggcatcggc aagcgaattg gtaataaatg cgctaaaacc ccatatggat gtagtgttgg
    14461 taggaagcaa aacacacgga aaaccggttg gaatgtacac attccagttt aacgatccaa
    14521 gtatcgactg ggctattgtt ccggtatgtt tcagcataag gaactccaac aatgagggcg
    14581 attactacga tggtattccc gtaactgttg aagctgccga tgatatattt acaccttttg
    14641 gagaagttac tgaaagtagc ctgaatagtg cactcaacta ccttggactt ggaacaaagg
    14701 cagtaacaaa aagttatgta acagctcaac caataacagg gaagggacta tacgaagaaa
    14761 ttggcgcttg gtaaccaact gaaatcattg gtatggaagg aatccaatta t
//
LOCUS       DAOOWJ010000065        14792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4083_length_14792_cov_8.113388, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000065 DAOOWJ010000000
VERSION     DAOOWJ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14792
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4083_length_14792_cov_8.113388"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLW22_07560"
     CDS             complement(<1..740)
                     /locus_tag="PLW22_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74599.1"
                     /translation="MAKGFLLVAIFSILLLNQQPLIASINDDFDHRLYVAFISGNMAD
                     WGKVLSELEAIASKNPSATNRFRLLHAQYGYIGYLLGVNKTSIAKQLIDKAEKQANIL
                     ASIPTYQSTAKAMQAALLAYRISISPYKAPFLGPKSASLIDEALALNPTNYFALIEKG
                     NARHYAPSIVGGNPTEAVELYRKAIELIEKENPASKPQKWWYINTYTQMGLAAQKAGN
                     KDLAKKIYLTILGMEPNYKWVREELLPKV"
     gene            complement(751..1848)
                     /locus_tag="PLW22_07565"
     CDS             complement(751..1848)
                     /locus_tag="PLW22_07565"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU74600.1"
                     /translation="MILFYLVLPIVIFAIIRFLVALFNYFTQPYLQSYPIISSETVSV
                     LIPARNEEANIQNLLADLQSQSYTNIEVIVYNDLSTDATGKIVEQFVKADNRFRLING
                     SGLPEGWLGKNNACHQLALQANGNYLLFLDADVRVEKSFVGQALAYMQHKKLHLLSFF
                     PYQVLGSFGEAITVPVMQWILLSLLPLKLVLWSRRRSLSAANGQMMMFKAETYRKHRF
                     HEIYRSNPVEDILIAREMKRFRYRISTLLGTPGDIRCRMYASGSDAIEGFSKNVCEFF
                     GGNRNVMFSFAAITTLGPFLVMFFMPFPLVFTYFFSVIMARLFIADLSKQNVFKAVIL
                     FPYQQISFLQMVIRWEKNRKRGTLIWKGRKI"
     gene            complement(1845..2468)
                     /locus_tag="PLW22_07570"
     CDS             complement(1845..2468)
                     /locus_tag="PLW22_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU74601.1"
                     /translation="MIKPAHNALYERFFNAYFNYILRRNFREIRIEGDFVPDNRPILL
                     VPNHFSWWDGFFAWKLNTMLLHKKFYLMMLESELSRHRFFAKLGAFSIAPTSKGMLES
                     LRFATEVIKQPNNLLVFYPQGKLYSQHHMALEFQKGIERIVEQSDNFRLLMCANLIDY
                     YAYPKPSLTIYLQEYERLQDFSLTQFQLTYNLFLKQSIHKQDKIHRQ"
     gene            complement(2482..3120)
                     /locus_tag="PLW22_07575"
     CDS             complement(2482..3120)
                     /locus_tag="PLW22_07575"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOU74602.1"
                     /translation="MNRLAGYFRVIIPIAYLIGIVGISIKINNPQYFKVTSIFVPVSF
                     LILMLFHKPHSLRFWITLASIGLLGFLVEAIGTNTGLIFGQYTYGSPLGFGVFGTPLN
                     MAINWMMLVYLVAFVLQKWNINITLKSGVSAIILTLFDWVLEPVAIRLDMWRWDLQTP
                     PFHNYIGWFLFSFIVFSIFYRFATKLHNPLANTFAVMHSLFFIVLNLIFRLI"
     gene            complement(3122..4576)
                     /gene="crtI"
                     /locus_tag="PLW22_07580"
     CDS             complement(3122..4576)
                     /gene="crtI"
                     /locus_tag="PLW22_07580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOU74603.1"
                     /translation="MQTVSTVGAGIGGIATAIRLARKGYRVKVFEQQPNAGGKLNELR
                     LSGFRFDAGPSLFTLPQLVDELIGSRDYSIFSYQKLELITRYFFPDGTVLDAWAEPER
                     FAEEVSQKFNDKHTAVLRYLKESRELYELTANTFVYSPFPQLKNFFTLQSLKLAMNPK
                     KLKAFETMHEVNCKHFSDHRTIQLFDRYATYNGSNPYLAPGTLTMIPHLEHNIGAYFP
                     TAGMYAITTEMVKIAQNLGVEFEYNAHVDKVDIRNGRVVGIKVNGSSAESNYVVNNTD
                     IFTAYKTLLHEVPLASRLAAQQPSSSALIFYFGVKGIHPKLDVHNILFTSDYYSEFKA
                     MEQGSIFNDPTIYIFITSKRIAADAPDGHENWFVMINVPPNTGQDWDELRLKAREAIL
                     VKIKRMLGIDLASLIVAEDYLDPVRIEKRTGSFRGSLYGISSNNRMAAFNRHPNRSRN
                     VKGLYFVGGSVHPGGGIPLCLASAKIVDTYFKPL"
     gene            4683..6170
                     /gene="crtI"
                     /locus_tag="PLW22_07585"
     CDS             4683..6170
                     /gene="crtI"
                     /locus_tag="PLW22_07585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOU74604.1"
                     /translation="MARKVVIVGTGLGGLSTALRLTTLGFEVEMIEKYHQPGGRLNKL
                     QHNGYTWDMAPTFFSMSYEFEELIDFCGIERPFDFFELDPLYKVNFSNSKKFFTIHKD
                     IHKLAREFEGIEDDFAEKMYQYLEKTGKLFHDVENRIVRRNFNSLLSYALGLASVPWG
                     HAPLMVKSMWDELNRRFTSYEVKVIFSLVAFFLGATPFDTPAVYSILTYTELKHDGYH
                     NVRGGMYKIVDGIVDILSKRGVKFHYNTEIVDFKYNNKKLTGVVDQNGNIWGADIFVV
                     NADAAWFRGAILKRKAYSENKLDRMKWTLAPFTVYLGVKGKIDGLSVHNYFLGTNFKE
                     YSSKIFKNQVSLEKPYYYVNVPSKANPEFAPGSNESLFILCPVPDLRYKPDWTDSHEL
                     AQNIIVDLSERVNYDIAGNVETQIIYNPAHWQKMFNLYKGSGLGLAHDLNQIAYMRPH
                     NTDEIFKNLFYVGSSTIPGTGLPMAVISSKLTVEQIVKRYDDGKN"
     gene            6151..6999
                     /locus_tag="PLW22_07590"
     CDS             6151..6999
                     /locus_tag="PLW22_07590"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOU74605.1"
                     /translation="MMTERIELYNNVCLECSKTTTKRYSTSFSLGTRLFDKPTRDAIY
                     AIYGFVRFADEIVDSFHPYNKAVLLNRFKTETYKAIEEGISINPILQSFQLVVNKYSI
                     DQHLIDAFLQSMEMDLNPTIYDRDKISEYIYGSAEVVGLMCLKVFCRGNQQKYEKLYQ
                     PARRLGAAFQKINFLRDMGEDFNDLGRVYFPNVDFNNFTPEKKLEIETDIQSDFDAAL
                     EGIKELESNSKLGVYVAYRYYLSLFHKIKRIPPHKLLGKRFRISNTKKALLMASSWAG
                     VKIGVI"
     gene            7407..8675
                     /locus_tag="PLW22_07595"
     CDS             7407..8675
                     /locus_tag="PLW22_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOU74606.1"
                     /translation="MNGELEFEPLGQAEYCDDDEPPSGGKYDRYVDSANYLEVKNHSA
                     KAFPINDNTQSFIKKFYPGVTNEEWNSWRWQLKNSITSIKHLIKFIELTPQEIEAIDN
                     PHHSLPLRITPYYASLIGRDNETDPIRRCMVPTINELLLSKGESSDPLEEQHYSPVPN
                     LVHRYPDRVLFLTTGFCSAYCRYCTRSHMVAKDKCHVGVEAWQPALEYIRNHSEVRDV
                     LVSGGDPLTMSDSHIEYLLQNLRAIKHVEIIRIGTKVPVVLPQRITHNLLRILKQYHP
                     LYISIHFAHPAELTPETAEACARLANIGIPLGSQTVLLKGINDNVDTMKRLMQGLLKI
                     RVRPYYIYQCDPIPGSNHFRTKVSKGIEIIKGLRGFTTGYAVPTFVIDAPGGGGKIPL
                     LPEYFQGEIDGKVILKNYEDKTFYYPNPID"
     gene            8696..9682
                     /locus_tag="PLW22_07600"
     CDS             8696..9682
                     /locus_tag="PLW22_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74607.1"
                     /translation="MLKVGLTYDLRSDYLKLGYSDEETAEFDSEETIEAIDNTLITLG
                     YKTQRIGNIFSLIDRINKGERWDLVFNICEGMHGIGREAQVPALLDAFNIPYVFSDPL
                     VLSLTLHKGQTKRVVRDCNIPTADFAIVESIQDIKHINLPYPLFAKPVAEGTGKGIDG
                     RSIITSNSDLQLVCQNLLARFQQPVLVETYLPGREFTVGIVGTGKNAKSIGVMEITIT
                     SKAKEQTYSFHTKENWKGVVEYTMASGEIEKICADTALKAWRCLGCRDGGRVDLKMDA
                     SGIPNFIEVNPLAGLNPSHSDLPMLARNNGIDYRQLIKLVVDSALIRLNGSV"
     gene            9679..10668
                     /locus_tag="PLW22_07605"
     CDS             9679..10668
                     /locus_tag="PLW22_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOU74608.1"
                     /translation="MNLKDLKAVILYNELSANAGPDEADVLDQVNVVSNALTTLGIMH
                     STLEFSLNLEKVQEKLIALKPDFVFNLVESVNNKGNLIFLAPALLNSLSIPFTGGSLE
                     TIFLTTSKTLTKELMSKASLPTPAWFHGKGKFTAEKGKKYIVKPIWEDGSLGLDENCV
                     FDATDPNLLKHIETLNFNTHFIEEYIDGREFNISILAGPKGPTVLPPAEIIFENYATT
                     KPKVVGYKAKWDESSYEYLHTFRTFDFNPSDDELIKKLQKICIDCWSAFNVNGYARVD
                     FRVDQNGNPYILEINVNPCISPDSGFYAACQKASIPFNEAINRIIHDIPSFKP"
     gene            10672..11157
                     /locus_tag="PLW22_07610"
     CDS             10672..11157
                     /locus_tag="PLW22_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU74609.1"
                     /translation="MQGLTIRTEPVSKDIESIRNIVSSSGFFQDYEIPVAIELVEERL
                     EKGEASGYFFVFAEVNGETISYACFGPIPCTKNSYDLYWIATRNDFRGNGVGKVVLKE
                     AEKEIKRLGGRAIYIETSSKPLYKPTQKFYLDNGYRIEAILKDFYDLNDDKLIFTKQL
                     D"
     gene            11497..12555
                     /locus_tag="PLW22_07615"
     CDS             11497..12555
                     /locus_tag="PLW22_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU74610.1"
                     /translation="MRRKIFTYHQLLLVLLTVASISCSKSDKGKKPSDGRSRQAIVVD
                     GIIVGYSEYERTITVPGTILPNEWVELRSEMAGKIVELNIAEGAMVRKGQLLARINDA
                     DIKALLQRRVIEEKLAADDEQRKKRMLEINAISVQEYETSLNKLEAIRADIAQSKAML
                     EKAEVRAPFEGRIGLRYVSPGAFVSVNTTIATLVQDNPLKLEFAIPERYASLVSNNME
                     VKFTAGDAQTQYPAKVYATDAQIDPETRSLKVRAIAENKSNQIIPGSYVRATLVFEKS
                     PRSILIPPRAIVPDMDIQNVFIYSQGKAKRIQVRLGERTGTTVEVIDGLNAGDSLIIS
                     GLTSVRDGMPVSINIKQN"
     gene            12583..>14792
                     /locus_tag="PLW22_07620"
     CDS             12583..>14792
                     /locus_tag="PLW22_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU74611.1"
                     /translation="MNISTVSINRPVLASVVSIVIILFGVIGFTYLGVREYPSVDPPV
                     VTVSTSYVGANSAVIEAQITEPLEAQINGIDGIKNITSSSSDGRSSITVEFELGIDMD
                     NAANDVRDRVSRAIRNLPPDADPPIISKADANAETILTVTVQSTKRDLLELTDIANNV
                     VKERLQTIQGVSLVNIWGEKRYAMRLYLDPKKLAAFNVTPSDIRNALSRENVELPTGM
                     IEGYQTTLSIRTLGRLETPEEFNNLIIKYTNNVPVRVKDVGKADYAPENERTLLRGNG
                     LIPMVGVALTPQPGANYINIADEAYKRVEQLRKELPEDITLNYAYDSTINIRKAISEV
                     EETILLAFMLVVLVIFIFLRNWRTTLIPIIAIPISLIGTFFVMYLFGFTINILTLLGV
                     VLATGIVVDDAIVVMENIYSKIEHGMDNYKAGYTGSKEIFFAIISTTITLVAVFMPIV
                     FLQGITGRLFREFGVVVAAAVLISSIVSLTLTPMMSTRLLTRTQKEGKMMAAIGQGIS
                     WLSEYYGRSLQSFINRRWLAFVVMGISLFIIFIIGSRIPSELAPMEDKGRLSINVTAP
                     EGTSFEAIDEYIYDIASYVDTIPEKKAMIALSAPGWGGGTNRGFVRLLLTQPNERERS
                     QAQLADQISSYVRTKTFAQSYVIQDQTISTGRGGGLPVQFVVQAPNFEKLKQVVPEFL
                     ARAQADERFQVVDVNLKFNKPELVVEIDRDKARASGVTVRDVADALQLYFSGQR"
BASE COUNT         4498 a         3093 c         3076 g         4125 t
ORIGIN      
        1 acttttggta acagttcctc ccttacccac ttgtagttag gctccatgcc tagtatggtt
       61 aggtagattt ttttagctaa atctttattt ccggcctttt gtgcagctag tcccatttgg
      121 gtatatgtgt ttatatacca ccatttctgg ggcttactcg ccggattttc tttttcaatt
      181 agctcaatcg ctttacggta aagctcaacc gcctcggttg ggttgcctcc cacaattgag
      241 ggtgcgtagt gtcgtgcatt ccccttttcg ataagcgcaa agtaattggt ggggtttaac
      301 gccaatgcct cgtcgattag cgatgcgctt ttaggcccaa gaaaaggcgc tttgtagggg
      361 cttattgaaa tccgataagc caacagggct gcctgcatgg ctttggcagt tgattggtag
      421 gtaggtatgc tggctaaaat attggcctgc ttttcagctt tatcaataag ttgctttgct
      481 atgcttgttt tgtttacacc cagcaggtat ccaatatagc catactgtgc atgtaaaagt
      541 cgaaatcgat ttgttgccga tggatttttt gaagctatag cctcaagttc acttaacacc
      601 ttaccccaat cggccatgtt accggaaata aaagcaacat agagccggtg gtcgaaatca
      661 tcgttaatac ttgcgattaa cggttgctga ttaagcaaca aaatggaaaa gatggcaact
      721 aacaaaaatc ctttagccat agaaatgcta ttatattttt cttcccttcc atatcagagt
      781 tccccgttta cgattttttt cccagcgtat caccatttgc aggaatgaaa tctgttgata
      841 tggaaaaagt ataacagctt taaaaacatt ttgtttggat aagtcagcaa taaacaaacg
      901 cgccattatg accgaaaaaa agtaagtaaa aaccaacggg aaaggcataa aaaacataac
      961 caggaaaggc cctaatgtgg taatggcggc aaagctgaac attacattcc tattgccgcc
     1021 aaaaaattca caaacgtttt ttgagaaacc ctcaatggca tcgctaccgc tagcatacat
     1081 cctacagcgt atatcgcctg gcgtgccaag tagggtggaa attcgatacc taaatcgttt
     1141 catctctctg gcaattagaa tatcctccac cggattgctt ctatatattt catggaaacg
     1201 atgtttacgg taggtttctg ccttaaacat catcatttgt ccgttggctg ccgaaagtga
     1261 ccgacgacgg ctccacaaaa cgagctttaa gggcaataac gacagtaaaa tccactgcat
     1321 tacaggaaca gtgatggcct caccaaatga gcctagcacc tggtatggga aaaacgatag
     1381 tagatgaagt ttcttatgct gcatgtaagc caaggcttgc cccacaaaac ttttctccac
     1441 cctaacatcg gcatcaagga ataaaaggta attgccattt gcttgtaagg caagctgatg
     1501 acatgcatta tttttcccca gccaaccctc cggcaaacca ctgccgttta tcaacctaaa
     1561 tcggttatcg gctttcacaa actgctcaac aattttaccg gtggcatcgg tcgataaatc
     1621 gttgtaaaca attacctcaa tattggtata gctttgcgat tgcaaatcgg caagcaggtt
     1681 ttgaatattt gcctcctcat tgcgtgcagg aataagcacc gaaacggttt cggacgatat
     1741 tatcggatag ctctgcaggt agggttgagt aaagtagttg aaaagcgcaa ccaaaaaccg
     1801 aataattgca aaaattacaa taggaagcac taggtaaaac agtatcattg tctgtgtatt
     1861 ttgtcctgtt tgtgaatgct ctgttttagg aatagattat aggtaagctg gaattgagtt
     1921 aagctaaaat cctgtagccg ctcatactct tgtaaatata tggttagcga tggttttggg
     1981 taggcgtagt agtcaataag attagcgcac atcagcagcc tgaagttgtc cgattgctcc
     2041 actatacgtt caataccctt ctggaactca agtgccatgt gatgctggct atacagtttg
     2101 ccttgaggat aaaatactaa aaggttattg ggttgtttta taacctcagt ggcaaacctt
     2161 aagctctcaa gcattccttt tgatgtggga gcaatggaaa aagcccccag ctttgcaaaa
     2221 aaacgatgcc tgctaagctc actttcaagc atcatcagat aaaacttttt gtgtagcagc
     2281 atggtattaa gcttccatgc aaaaaaacca tcccaccagc taaaatggtt tggtaccagt
     2341 aaaattgggc gattatcagg aacaaaatcg ccctcaattc ggatttccct gaaattacgt
     2401 ctcagaatat agttaaagta agcattgaag aatctctcat acaatgcgtt atgtgccggt
     2461 ttaatcactt cgataccaaa tttaaatcaa cctaaaaata aggttaagaa cgataaaaaa
     2521 caaactatgc attaccgcaa aagtattcgc aagcgggtta tgcaattttg ttgcaaaccg
     2581 gtaaaagatg ctgaatacta tgaacgagaa taaaaaccaa ccgatatagt tatgaaaggg
     2641 tggtgtttgt aaatcccacc gccacatatc gagccggatg gctaccggtt caagcaccca
     2701 gtcaaacaac gttaggatga tggctgatac ccccgatttc agagttatgt taatgttcca
     2761 tttctgcaac acaaaggcaa caaggtaaac aagcatcatc cagttaatgg ccatattcag
     2821 gggtgttcca aacactccaa aacctaaagg actgccatac gtgtattggc caaaaatcag
     2881 acccgtgtta gttccaatag cctcaactaa aaaacccagt aagccaatcg atgccaaagt
     2941 aatccaaaac cttaaactat gaggtttgtg gaataacatt agtatcaaaa acgatactgg
     3001 aacaaaaatg gaagtaacct taaagtattg tggattattt atctttattg agatacccac
     3061 aatgccaata aggtatgcta tggggattat caccctaaaa tatccagcaa gccgattcat
     3121 gctacaaggg tttgaaatag gtatcaacaa tcttggccga agctaggcaa agcggaattc
     3181 ctccgcctgg gtgaacgctt ccgccaacaa aataaagacc tttaacattt ctacttctgt
     3241 ttggatggcg gttgaacgct gccattctgt tattggaact aattccataa agcgaaccgc
     3301 ggaacgaccc ggtacgtttc tcaatccgaa cgggatccaa atagtcctcg gctacaatta
     3361 aactagccaa atcaatacct aacatacgtt taatctttac aagtatggcc tcgcgagctt
     3421 tgagccttag ttcatcccag tcctgcccag tatttggtgg tacatttatc atcacaaacc
     3481 agttctcatg cccatcggga gcatcagcgg caatacgttt tgaagtgata aatatgtaaa
     3541 tggttgggtc gttgaaaatt gaaccttgct ccatggcctt aaattccgag tagtaatcgg
     3601 acgtaaagag gatattatgt acatcgagct tagggtgtat acccttaacc ccaaagtaaa
     3661 agattaatgc cgatgatgat ggctgctgtg cagccaatcg gctggctaaa ggaacctcgt
     3721 gtaaaagcgt tttgtatgcc gtgaaaatat cggtgttatt gaccacatag ttgctttcag
     3781 cgctacttcc atttacctta atgcctacca ctcggccatt tcggatatca accttatcca
     3841 catgagcgtt atattcaaat tcaacaccca ggttttgggc aattttaacc atttcggtgg
     3901 ttatggcata catccctgct gtaggaaagt aagctccaat attatgctca aggtggggta
     3961 tcatggtaag cgttccgggt gcaaggtatg ggtttgagcc attgtatgtg gcatacctat
     4021 caaagagttg gattgttcgg tgatcagaaa agtgcttaca gtttacctca tgcatggtct
     4081 cgaacgcttt cagctttttt ggattcatgg caagtttaag cgattgcagg gtaaaaaagt
     4141 tctttagctg agggaaagga ctatacacaa aagtattggc tgtaagttcg taaagctctc
     4201 tactctcctt taagtaacgg agaactgccg tatgcttatc gttaaacttt tgggatacct
     4261 cttcggcaaa gcgttcaggc tcagcccatg catcaaggac agtgccatcg ggaaaaaagt
     4321 aacgggtgat tagctcaagt ttttggtaac tgaaaatgga atagtccctg cttccgataa
     4381 gttcatccac cagctgtggt aaagtaaata acgatggccc cgcatcgaac cgaaaacctg
     4441 aaagtcttag ctcattcaat ttaccaccag cattgggttg ctgttcaaac actttaaccc
     4501 tatacccctt gcgtgccaat cggattgctg tggcaattcc gccaattccc gcccctactg
     4561 ttgaaaccgt ttgcatactt tttaaacaaa ataaggcatt tctggttaat actcatggta
     4621 aatttaatta aaattgtata tttaatgttt gttaatgtgt tttaaatagt aaataagcat
     4681 ttatggcaag gaaagttgta attgttggga cagggcttgg tggtttatca actgcactta
     4741 ggctaacaac gcttggtttt gaagtggaaa tgattgaaaa gtaccatcaa cccggaggcc
     4801 gtttaaataa gctgcaacac aatggctaca cctgggatat ggcgccaact ttcttttcca
     4861 tgagctatga atttgaggaa cttattgatt tttgtggaat agaaagacct ttcgactttt
     4921 ttgagctcga cccgctctac aaggtaaatt ttagcaattc caaaaagttt tttacaatac
     4981 ataaagatat tcacaagctg gccagggagt ttgaaggaat tgaagatgat tttgcagaaa
     5041 aaatgtacca atacctggaa aaaacaggta agctattcca cgatgtggaa aacagaattg
     5101 tgcgccgcaa cttcaacagc ttgctttcat acgcattagg gctagcctcc gtaccgtggg
     5161 ggcatgctcc tctaatggtt aaatcgatgt gggatgagct taaccgaagg tttaccagct
     5221 acgaggtgaa agtgattttc tcattggtgg catttttcct gggggcaaca ccattcgata
     5281 cccctgcggt ttactccatt cttacctata ctgaactgaa gcacgatgga taccataatg
     5341 ttcgcggtgg tatgtataag attgttgatg gcattgttga tattctctca aagcggggtg
     5401 taaagttcca ctacaacacc gagatagttg attttaaata caacaacaaa aaactaaccg
     5461 gtgttgttga ccaaaatggc aacatatggg gtgctgacat ttttgttgta aatgccgatg
     5521 cagcatggtt taggggtgca atacttaagc gtaaagccta cagcgagaac aagctcgata
     5581 gaatgaaatg gactcttgcc ccttttactg tttacctggg agtaaaaggt aaaattgatg
     5641 gtttaagtgt tcacaactac tttttgggaa ctaactttaa ggaatacagc tccaagattt
     5701 ttaagaatca ggttagctta gaaaaacctt actactacgt taatgtccca tctaaagcaa
     5761 accctgagtt tgcccctggt agtaacgaaa gcctttttat tctttgccct gttcccgact
     5821 tacgctataa acccgattgg accgatagcc atgagttagc ccaaaatata atagttgacc
     5881 tcagcgagag ggttaattac gatattgccg gaaatgttga aacgcaaatt atttacaacc
     5941 ctgcgcactg gcaaaaaatg tttaacctgt acaagggcag cggtcttggc cttgcccacg
     6001 atttaaacca aatagcttac atgcgacccc acaataccga tgaaattttc aaaaacctat
     6061 tctatgtggg ttcaagcacc attcccggaa cgggcttgcc aatggcagtt attagttcaa
     6121 aattaacagt tgagcaaatt gtaaaacggt atgatgacgg aaagaattga actatacaat
     6181 aatgtttgcc ttgagtgtag caaaacaaca accaaacgct acagcacaag tttttccttg
     6241 ggcacacgcc tttttgataa acctacccgc gatgccattt atgccatata cggttttgtt
     6301 cgttttgccg atgagattgt ggactcattc cacccctaca ataaggctgt actccttaat
     6361 cgctttaaaa ccgaaaccta caaagcaatc gaggaaggta tctcaataaa cccaatacta
     6421 caaagctttc agctagtggt aaataaatac agcattgacc aacacctgat agatgccttt
     6481 ttgcaaagca tggaaatgga ccttaaccca actatctacg atagggataa aataagtgag
     6541 tatatttatg gctcagccga agtggtaggg cttatgtgct taaaagtttt ttgcaggggc
     6601 aaccagcaga agtatgaaaa gctttaccaa ccagcccgga ggcttggcgc tgcattccaa
     6661 aaaatcaatt tccttcgcga catgggcgag gattttaacg atctgggtag ggtttacttc
     6721 cccaatgttg atttcaacaa ctttacacct gaaaaaaaac tagaaattga aaccgatatc
     6781 caatcggatt ttgatgctgc ccttgaagga atcaaagagc ttgaaagcaa ttccaagcta
     6841 ggtgtttacg tagcttaccg atactacctc tcgctcttcc acaagattaa acggattcct
     6901 cctcacaagt tgcttggtaa acgtttccgt attagcaaca caaaaaaggc attactcatg
     6961 gcaagcagct gggcaggggt taaaattgga gtgatttaat gaactacttt cactaatcct
     7021 atggggcttt atgtgctttt ttaatttttt caattttgaa gtataatcaa aagtctatag
     7081 gtcttgcaca aaaatagatt tgattcactt aagtaacctt tttagctatc taaaaattta
     7141 atgtcacccc aattattcgc taactgttca taaatccttt aggcctgtta atattttttt
     7201 atcaacaaac caaatacaca actatctgtt tatcttcact ttagtacaag cattacgttt
     7261 aaaaataaca ttttttaata ctgacattta cataagagcc ttcaagggca aacatcgtat
     7321 ttaacaaatt ccaatatttg aatataattt ggctatagga aatcaaggct aagcaattcg
     7381 ataacaacac tttaaagact aaaactatga atggtgaact agaattcgag ccgcttgggc
     7441 aagcagaata ctgcgacgat gacgaacctc caagtggtgg taaatatgac aggtatgtag
     7501 attctgctaa ttatttagag gtcaaaaatc attctgctaa agcgttccct ataaatgata
     7561 acactcaaag ttttattaaa aaattttatc ccggagtaac taacgaggaa tggaatagct
     7621 ggcgatggca gcttaaaaat agcataacat cgataaagca tcttatcaaa tttatagagc
     7681 taacaccgca agaaattgag gctattgata atccgcatca ctcattacct ttaaggataa
     7741 ccccatacta tgcaagctta ataggtagag ataacgaaac ggatcctatt aggcgatgta
     7801 tggttccaac cataaacgaa ctattacttt ccaaaggtga atcatcggat ccgctggaag
     7861 aacaacacta tagccctgtg ccaaatctgg tacatcgcta tcccgatagg gtcttattcc
     7921 taaccaccgg cttttgctct gcttactgcc gatactgcac ccgttcgcat atggttgcaa
     7981 aggacaagtg ccatgttggg gttgaggcat ggcaacctgc ccttgagtat atcagaaacc
     8041 atagcgaggt aagagatgtt ctagtatcgg gtggtgatcc tttaaccatg agcgattccc
     8101 acatcgaata cctattgcag aaccttaggg caattaagca tgttgagatc atccgaattg
     8161 gcaccaaagt gcccgtagtt ttgccccagc gaataacaca taacttacta cggatactca
     8221 agcaatacca tccactttac atcagtatcc attttgcaca cccagccgaa cttacccccg
     8281 aaacagctga ggcttgtgca cgattggcaa atattggaat acccttggga agtcaaaccg
     8341 tattactaaa aggtattaac gacaacgttg ataccatgaa acgattaatg cagggccttc
     8401 ttaaaattag ggtccgtcct tactacattt accagtgcga ccccattccc ggatcgaatc
     8461 actttagaac aaaagtctca aaagggattg aaataatcaa aggtttgcgt ggattcacaa
     8521 ctggctatgc tgtgccgacc tttgttattg atgcccccgg tggaggtgga aaaattcctt
     8581 tgttaccgga atacttccag ggcgaaattg atggcaaggt gattttgaag aactatgagg
     8641 ataaaacctt ctactacccc aacccaattg attaaaaact gattaatctg gcattatgtt
     8701 aaaagtggga ttaacctacg atttacgtag cgattacctc aagcttggct attccgatga
     8761 agaaaccgct gaatttgact cggaagaaac aattgaagca atagacaata cactaatcac
     8821 actggggtat aaaactcaac gtattggcaa tattttttct cttatcgata ggattaataa
     8881 aggcgagcgg tgggatttag tatttaatat ttgtgagggt atgcatggaa ttggtcgtga
     8941 ggctcaagtt ccggcattgc tcgacgcctt taatattcct tacgtttttt ccgatccgtt
     9001 agttctgtcg ctcacacttc ataagggaca aacaaaacgc gttgtaaggg attgtaacat
     9061 tccaactgcc gattttgcca ttgttgaatc aattcaggat attaagcata tcaatttacc
     9121 ctaccctctt tttgccaaac cagttgccga gggaacgggt aaaggtattg atggtaggtc
     9181 aattattacc tcaaattccg atttgcaact ggtttgccaa aatcttttag caagatttca
     9241 acaaccggtg ctggtggaaa catacttacc cggaagggaa tttaccgtag ggattgtagg
     9301 cactgggaaa aatgcaaaat caattggagt aatggaaata accattactt caaaagccaa
     9361 ggaacaaacc tactcgtttc ataccaagga gaactggaag ggcgtagttg aatacaccat
     9421 ggcttcgggt gaaattgaaa agatatgtgc ggatacagca ctaaaagcat ggcgctgctt
     9481 agggtgcagg gatggtggac gggttgacct gaaaatggat gcttcaggta ttccaaattt
     9541 tattgaggtt aaccctttgg cagggttaaa cccttcgcac tccgatttac ctatgctcgc
     9601 ccgtaataat ggaattgact ataggcaact catcaagctt gtagttgatt ctgcattgat
     9661 aagattaaat ggtagtgtat gaatcttaaa gatcttaaag cagttatcct atacaacgag
     9721 ctttcggcaa acgctggccc cgatgaagcc gatgtcctag accaagtaaa tgtagtttcc
     9781 aatgctctaa ccaccttggg tattatgcat tctacgctag agttttcact taaccttgaa
     9841 aaagttcagg aaaaactgat agcgttgaag cccgattttg tgtttaacct tgttgaatca
     9901 gttaacaata aagggaatct tatttttctt gccccagccc tattaaattc actttcaata
     9961 ccatttacag ggggctcact ggaaaccatt tttctgacta cctcaaaaac ccttaccaag
    10021 gaactaatga gcaaggcaag cctaccaaca cctgcttggt ttcatggcaa aggaaagttt
    10081 acggcagaaa aagggaaaaa atatattgta aaaccaattt gggaggatgg ttcgcttgga
    10141 cttgacgaaa actgcgtttt tgatgcaacc gatcccaacc tattaaaaca cattgaaaca
    10201 ctaaacttta acacacactt tattgaggag tatatcgatg ggcgtgagtt taacatttcc
    10261 attctggcag gccccaaggg ccctaccgtt ctaccccccg ccgaaattat cttcgaaaac
    10321 tatgcgacaa ctaaacctaa agtagttggt tataaagcca aatgggacga aagtagctat
    10381 gaataccttc acacctttag gacattcgat tttaatccca gcgatgatga gctaattaag
    10441 aaactacaaa aaatatgtat tgattgctgg agtgccttta acgttaatgg atatgcccgt
    10501 gtggatttca gggttgacca aaatggaaat ccttacatcc ttgaaatcaa tgtaaaccca
    10561 tgcatttcgc ccgatagtgg attttatgca gcatgccaaa aggcatcaat accttttaac
    10621 gaagcaataa atagaataat acatgatatt ccatcgttta agccgtaagc catgcaagga
    10681 ctaactatca gaactgagcc ggtatccaag gatattgaat ccatcagaaa cattgtcagt
    10741 tcatccggat tttttcagga ttatgagatt cctgttgcta ttgagttagt ggaagaacga
    10801 ttagaaaaag gtgaagcaag tggttacttc tttgtttttg ccgaggtgaa tggcgaaacc
    10861 atttcgtatg cctgctttgg tccaattccc tgcacaaaaa acagttacga cctttactgg
    10921 attgcaacca gaaacgattt tcgggggaat ggagttggca aagtagtact gaaggaagcc
    10981 gaaaaggaaa tcaaaagact tggcggaaga gccatatata ttgaaacttc ctcgaagcca
    11041 ctttataaac caacccaaaa attctacctt gacaacggct acaggattga agccatacta
    11101 aaggatttct acgatttaaa tgacgataag ctaattttca caaagcaact tgactgattt
    11161 tgttagccat taaaacaaat gaaaagcccc gcagaaagcg gggcttagta ttcataccct
    11221 ttaatccgga accacaactt tctgtactac catttccgat tgaccaccgt cgctggtcat
    11281 cactacaatc ccgtacgtgt ttgaaccctt taaaggtgat gtatcagtaa actcaaggat
    11341 atcgggtttt aaccctttca gttcaacaaa atctttattt aattaagcta ttatgccaaa
    11401 caaaatatga gtcaagttga aaattttaaa acattttttt gggttaatcc ttttagataa
    11461 aaattagtca ttaatttcag aaataaataa acggaaatga gaagaaaaat ttttacatac
    11521 catcaacttt tgcttgtgtt acttacagtg gcaagcatat cgtgcagtaa atcggataag
    11581 ggaaaaaagc ctagcgatgg tcgcagccgg caggcaatag tagttgatgg aattatagtt
    11641 ggatattctg aatatgaaag gaccatcacg gttcctggca ccattctccc aaacgagtgg
    11701 gttgagctac gctccgaaat ggcaggaaaa attgttgagc taaacatagc tgagggagca
    11761 atggtacgca aagggcaact acttgcacgg attaacgatg ccgatattaa agcgctactc
    11821 caacgaaggg taattgagga gaagcttgcc gccgacgatg agcaacgcaa aaagcgcatg
    11881 ctcgagataa atgccataag tgttcaggag tatgaaacat cgcttaataa gcttgaagcc
    11941 atcagggccg atatagccca atcgaaagcg atgttggaaa aagctgaagt gcgtgctccc
    12001 ttcgaaggac gaataggctt gcgctacgtt agtccaggtg catttgtatc ggtaaacaca
    12061 accattgcca cactggttca ggataaccca ttaaagttgg aatttgccat accggagcgt
    12121 tacgctagtt tggtatccaa taacatggag gtaaagttta cagccggtga tgcacaaaca
    12181 caataccctg caaaggttta cgctaccgat gcccaaatag accctgaaac acgctcgctt
    12241 aaggttaggg caattgccga aaataagagt aatcaaataa ttcctggctc ctacgtcaga
    12301 gcaaccctgg tttttgagaa aagtcctcgt tcaatactta taccgcctag ggcaattgtt
    12361 cccgacatgg atatccaaaa tgtttttatt tactcccagg gtaaagctaa gcgcattcag
    12421 gtaaggttgg gtgaacgaac aggtactaca gttgaggtaa ttgatggctt aaatgccggc
    12481 gatagtttaa taatatcggg tttgacctct gtgcgagacg gtatgcctgt tagcattaat
    12541 atcaagcaaa actaaaagtt ttaactaaac aaggttacca aagtgaacat ctcaacagtt
    12601 agcataaata ggccagttct ggcatcggtg gtttcaatag taattattct ttttggtgta
    12661 ataggcttta cttacctcgg cgttagggaa taccccagtg tagacccacc cgtggttacg
    12721 gtttccacat cgtatgttgg ggctaactca gcagttattg aggcacaaat cactgagcct
    12781 cttgaggctc aaataaatgg gattgacggt ataaaaaaca ttacatcatc aagctccgat
    12841 ggtcgtagta gtatcaccgt tgagtttgaa ctggggattg atatggataa tgccgccaac
    12901 gatgtgcgcg accgtgtttc gcgtgccatt cgtaaccttc cccccgacgc tgacccgccc
    12961 attataagca aagctgacgc caatgcggaa accattctga ccgttacggt tcaaagcacc
    13021 aaacgcgatt tacttgaact aactgacatt gccaataatg ttgttaagga acgattgcaa
    13081 accattcagg gcgtcagtct ggtaaatata tggggcgaaa aacgctacgc catgcgatta
    13141 tacctcgacc cgaaaaagct ggctgcgttc aatgtaacac cttccgatat tcgcaatgcg
    13201 ctttcgcgcg agaacgttga acttcctacc ggtatgattg agggttacca aaccactctc
    13261 tcaatccgaa ccctcggtcg actcgaaaca cccgaggagt ttaacaacct gatcatcaaa
    13321 tacaccaata acgtgcccgt tagggttaag gatgtaggca aagccgatta tgccccagag
    13381 aatgaacgaa cgctactccg tggtaatggg ctgatcccta tggttggtgt tgcgctaacc
    13441 ccacagcccg gtgcaaacta catcaacatt gccgatgagg cttacaagcg tgttgagcag
    13501 ctccgtaagg aattgcccga ggatatcacc ctaaattacg cctacgattc aacaataaat
    13561 atccgcaagg caatttctga ggttgaggaa accattcttc tggcgtttat gctcgttgtt
    13621 cttgttattt ttatttttct gcgaaactgg cgcactacct taattccaat tattgccatt
    13681 cccatttcgt taataggaac cttttttgtg atgtaccttt ttggttttac cataaatatt
    13741 ctaactctat taggtgtagt tcttgccact ggtattgtag ttgacgatgc cattgttgtt
    13801 atggagaaca tctactccaa aattgagcat ggcatggaca actataaagc aggatacaca
    13861 ggtagcaagg agattttctt tgccattatc tccacaacta tcactctggt tgcggtattc
    13921 atgccaattg tatttctaca aggcatcaca ggtcgtcttt tccgcgagtt tggagtagtt
    13981 gtggcagccg ctgtactaat ttcgtcaatt gtatcgctta cgcttacccc gatgatgagc
    14041 actcgccttt tgaccagaac gcaaaaggaa ggaaaaatga tggctgccat tggccaggga
    14101 atcagctggc tatcggagta ctacggaaga tcgttacaat cattcataaa ccgcaggtgg
    14161 ttggcatttg tggttatggg catctcacta ttcattattt ttattatagg cagccgtatc
    14221 ccttcggagt tggctccaat ggaggacaaa ggcagactat cgataaatgt tactgcacct
    14281 gagggtactt cgtttgaggc tattgacgag tacatttacg atatcgcctc gtatgttgat
    14341 acaatccctg agaaaaaggc catgattgct ctatcggctc ccggttgggg aggtggcaca
    14401 aatagaggat ttgttcggtt actgcttact cagcccaatg aacgtgaacg ttcacaggcg
    14461 caacttgccg atcaaatatc atcatacgtc cgcactaaaa cctttgccca gtcgtatgta
    14521 atacaggatc aaaccatcag caccggaagg ggtggtggtt taccggttca gtttgtggtg
    14581 caggcaccaa actttgaaaa gctaaagcag gttgtacccg aatttttagc ccgggcacag
    14641 gccgatgaac gcttccaggt ggttgatgtc aaccttaagt ttaataaacc tgagcttgtt
    14701 gttgaaattg accgcgataa ggcaagagcc tcaggtgtta ccgtaaggga tgttgccgat
    14761 gcccttcagc tttacttcag cggacagcgc ta
//
LOCUS       DAOOWJ010000066        14663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4131_length_14663_cov_8.626027, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000066 DAOOWJ010000000
VERSION     DAOOWJ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14663
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4131_length_14663_cov_8.626027"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            775..2823
                     /locus_tag="PLW22_07625"
     CDS             775..2823
                     /locus_tag="PLW22_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOU74612.1"
                     /translation="MKQFLSVPFACLLLVVAVYISSSYAYPQEGARTLAGKHSQSDSL
                     RGRNVPFDKGSARGVGRSATDTLKPNERNIYGYLGIQDPKSKSFAWYFDRETYDLVQT
                     QAFDSSLFLVHLILPGQKSLSPLTYLGNMGSPVQSDHFFERPVSYPFLFSKGYSSYEQ
                     PVLDRKQYNVRRPHTLLEYSTAGKRRNAEQNLRVFHTQNVNRYLNFGLQYDYFSTKGI
                     YERQLTRNNDFTAFASYYRHRVSAQGTFAYTYIRNQENGGLEDDRFIQDTVMEPNLVP
                     FRLKDASVEYRKRSFSGVVGYDIITKRVNDSLENLKSILTAKLILDANRYTRVYADTE
                     TDSSYYGNFYISTTSTHDSVYLATYQSTLLVELSQIAKYPGIPGLRAWVSGLMGSYYN
                     FTPDNFIYTYSNTKLNTSHFGLGVYSQSPYLSYSGAARFYMSGYRAADKELLGRLSIS
                     PWKSKELPYVAGELSITDREPDIFINRYFSNHYKWDNTFEKESRFRLSARLGADRFGF
                     EAGYSIEHILNYVYFDTLSLPAQEPKVTVTSAYIQKKFRLGWFNAVCKVIWQASTNAD
                     VLSLPTLAGFGALYVQYPVVKDVLTMQLGVSGFYRTEFYADAYNPALGVYHNQRVRKI
                     GNYPFVDVFLNARWKRANLFIKLDHANQGLIDNQYFTTLHYPYNPRMVKFGVSWMFYD
                     "
     gene            2903..4306
                     /locus_tag="PLW22_07630"
     CDS             2903..4306
                     /locus_tag="PLW22_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOU74613.1"
                     /translation="MVRKVIYLRNGLIFLAMLIFFSVSSCEKKGVKDEEVQRDLADIL
                     ADGKLVAVTDYNATSYFIYKGEPMGYQYDMLRDLATYLGVKLEIVTENDIDKSFEMLR
                     KGKVDIIASSLAITAQRKEEVDFTLPHGETSQVLVQRIPAENGTPAIQKPLELSGRLV
                     YVQKSSAGAQRLKNLSDEIGGGITVVELPNYDADKLIELVATGEIDYAVCDQSVALVK
                     SNYYKNINIDIQIGFPQKTAWAVRKTSVELRKKVDTWLEGYLKTAHYRNIELKYFAQE
                     RFNRRASSDYFFIYTGKISPWDEYFKKYSQGIDWDWRLLASLVYQESRFRHDVTSHRG
                     ASGLMQMMPQTAEYFGVDSTVGPAKQIEAGVRYIKWLEDRFAAYDIPKDEMVKFVLAS
                     YNAGIGHIIDARNLAQKHGRNPNVWDNNVEYFIRNKSQFVNDPVVRHGKLKGVETTNY
                     VKEIMERYQHYKNIIGE"
     gene            4370..5905
                     /gene="gltX"
                     /locus_tag="PLW22_07635"
     CDS             4370..5905
                     /gene="gltX"
                     /locus_tag="PLW22_07635"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOU74614.1"
                     /translation="MSNRRVRVRFAPSPTGPLHIGGVRTALFNYFFARQHGGDFILRI
                     EDTDSQRFVPGAEEYINEALNWCGIKIDEGVREGGPHAPYRQSERKHIYKQYADRLVE
                     SGHAYYAFDTPEVLDKMRAEAEAQGGAFTYNYAIRNGMENSLALPADEVKRRIERGDQ
                     WVIRFKMPENEEVVMHDLIRGEVRVNTSTLDDKVLFKSSDMLPTYHLANVTDDYLMEI
                     SHVIRGEEWLPSLPLHVLLYRALGWENVMPKFAHLPLLLKPSGNGKLSKRDGDKMGFP
                     VFPLLWKSADGEISRGYREDGYFPEAFVNLLALLGWNPGTDQELFSMDELIKLFSIER
                     VNKSGARFDPEKAKWFNHQYLIRKSDAEVAELFQPVLEQYNIDVPFDKLTRIVGLVKE
                     RVNFVHELWGQTSYFFERHLEYDQQAVAKHWKPETPDMITDVSETLKALDTWNSHEIE
                     TALKDLINAKGYGMGKVMNAVRLCLVGESKGPGIADICEILGRDETLTRLNQAILILN
                     GKK"
     gene            6527..7297
                     /locus_tag="PLW22_07640"
     CDS             6527..7297
                     /locus_tag="PLW22_07640"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOU74615.1"
                     /translation="MERNKLNYIETRRMVEDLSDSLTELVVESLLGAGQTVDTEKPYS
                     KLYEALRYFNPDTSQCQRQLEIVYQGFQGVLDTLSHDANFIYLNDPAAQSLEEVLLAY
                     PGFFAIAHYRLANLLHRVGVNILPRMITEYAHSKTGIDIHPAATIGQEFCIDHGTGVV
                     IGETTIIGQRVKLYQGVTLGALSVSKSLSATKRHPTIEDDVIIYAGSTILGGETVIGH
                     DSVIGGNVWLIKSVPPFSMVYHESKVIVQQGKFNHKMK"
     gene            7301..8212
                     /gene="cysK"
                     /locus_tag="PLW22_07645"
     CDS             7301..8212
                     /gene="cysK"
                     /locus_tag="PLW22_07645"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOU74616.1"
                     /translation="MKYLSIDKSIGATPHIRLSRLFPNAEVWLKDERRNPSGSIKDRA
                     AMAMVEDAERKGLLKPGSVIVEPTSGNTGIGLAMVAAVKGYRLILTMPESMSVERRVI
                     LKAYGAEIILTPAAQGMQGAIDKAKEFMAQLPSAWMPMQFANPANPNTHSLTTAKEIL
                     ADFPDGLDMVVAGVGTAGHITGVGRTLKEAFPNIQVIAVEPFDSPVLSGGQRAPHPIQ
                     GIGAGFVPQNLDRGVIDEIITVKADEAIECMRLLARTEGILAGISTGANIAAVKKLLS
                     VKQSNLRILTFAYDTGERYLSVKGIWD"
     gene            complement(8215..10794)
                     /gene="mutS"
                     /locus_tag="PLW22_07650"
     CDS             complement(8215..10794)
                     /gene="mutS"
                     /locus_tag="PLW22_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU74617.1"
                     /translation="MKQYLSIKRNHPDAILLFRVGDFYETFGEDAIKTSEILGITLTR
                     RANGAASFVELAGFPHHALDTYLPKLVRAGQKVAICEQLEDPKKAKTIVKRGITELVT
                     PGVSYNDNVLERKENNFLACVYLDKKAGGVAFLDISTGEFYTSEGNLDYVDKLINNFK
                     PKEILVERTKYKEFLEQFGSKHYVTRLDEWAFNEDAAREKLTKHFKTQSLKGFGVESL
                     QFGITAAGAILYYLDVTQHTQIGHIQSISRIDEDSFVWIDRFTSRNLELFSSINEGAK
                     TFIEVIDKTSSPMGARLLKRWVALPLKNTDSINDRLNVVELFVNNEDMRATLTDKIRD
                     MGDVERIISKAAVGRIQPREMVQLKNALSLIDEVKTICDQSAEPTLLRISEQLNPCHR
                     IRERIEQEIVPDAPMMLGKGQVIAKGVNAELDELRDILYSGKEYLLNIQQRESERTGI
                     PSLKVNFNNVFGYYIEVRNTHKDKVPADWIRKQTLVSAERYITQELKEYEEKILGAEE
                     KISELEQNLYQNLVHSLAEYVGSIQLNAYLIAQLDCLLSFAHCAVEFQYNRPQVNLDD
                     VIDIRQGRHPVIERMLPAGEQYIANDVYLDTREQQIIIITGPNMAGKSALLRQTALIV
                     LMAQMGSFVPAQAASIGVVDKIFTRVGASDNLSLGESTFMVEMQEAASILNNISPRSL
                     VLLDEIGRGTSTYDGISIAWAIVEYLHEHPSARAKTLFATHYHELNEMEKSFPRVKNY
                     NVTIKEVNNQVIFLRKLVRGGSEHSFGIHVARMAGMPPSVVKRANEILAELESSTQRQ
                     SLSKPVDELGTHREGYQLSIFQLEDPVLKQIRDQIKKIDINNLTPIEALNKLNEIKRL
                     TGL"
     gene            complement(12070..>14663)
                     /gene="secDF"
                     /locus_tag="PLW22_07655"
     CDS             complement(12070..>14663)
                     /gene="secDF"
                     /locus_tag="PLW22_07655"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOU74618.1"
                     /translation="GSEHFIDKFGKAFKQVAPNARLASIFNTPELRGRINFDSSDEDV
                     LKVLKQEAENAIDNSFNILRNRIDKFGVAQPNIQRLENSGLILIELPGIKEPERVRKL
                     LQGTASLEFWETYEYSELFNNFMAANSTLAEINKASQPTVTETTKKDETTEQKDELLS
                     KLKETEKTDTLKAAADMAKDYPLFSILQPNLYNNQPAPGPVVGYAHIKDTAQVNEILR
                     MPKIRALFPPDVKFRWGVKSPKWDKNKVFYELIALKVTTRDGKAPLDGGAITDAREAF
                     GDRKGEAEVDMEMNAEGARIWARMTGENVGRCIAIVLDDYVYSFPRVQTEIKGGRSQI
                     TGDFTINEAKDLANVLKSGKLPAPAHIVQEQLVGPSLGKEAINSGMNSFIVSFIIVLL
                     YMVIYYSRSAGLVADFALVTNLFFIMGVMASFGATLTLPGIAGIVLTLGMAVDANVLI
                     FERIREELRGGKGLSLAVKDGYRNALSAIIDGNVTTLLTGVILYLFGSGPIRGFATTL
                     MIGIITSMFCGIFITRLFLLWMEKRNIPMIFSTKLSENAFQKVKIDFFGLRKKAYFFS
                     GTLIAIAIISLAVRGLNPGIDFAGGCSYIVRFQNTVSTQEVSKSLKTYLGEAPEVKTI
                     GASNQVKITTKYKVADNDPNVDNEVDSLIYLGVKPFMANGANFSDFQHDYKQSSIKIG
                     PTIAHDIKRDAYIAVFFALVVIFVYILIRFRYWSYSAGGVIALFHDSLITIGLFSILY
                     YRVPFNMEIDQSFIAAILTIIGYSINDTVIIFDRIREYMGLHPKRTRRENMNSAINDT
                     LSRTFSTSFTTLIVLIPMFFFGGEVIRGFVFALIVGILIGTYSSIFVAAPIAYDLEKR
                     KEDKKNK"
BASE COUNT         4032 a         3203 c         3606 g         3822 t
ORIGIN      
        1 acgctacgcc tcagccagag cataaaaagc tttttgtacg agcctgaacc aaggggattt
       61 tttactacat ttactgacag ctaccgggcg gagcggcgta tgccgcacgc gtttaaaaac
      121 gagcgggagc gagggtaata aagcgaaaga agtaattgtc acggactaca agtccgcgcc
      181 agcaattggg gaatatgaaa aaggatgaat aaaaaaggaa gttataaaat aaaatattat
      241 tgtttagccc ctgttcagcc gaggctgtaa aaagaaataa acgaaccgcg ttcggcggcg
      301 gttgcaccgc cgtcgtgcta tcagcaacag gctgcacctg agttttttat aattcccccc
      361 agcacccgct cgcgcggatt taccttcggt gagctccctt cggtcggttg taatccgtgt
      421 gattgcagtt cggcaaagcc aatccttgcg gttattttaa gaggtgtttt caatgaggca
      481 cagctcgaat aagtaataaa gtgtgtaaga agtatttgtc acggactaaa agtccgcgcc
      541 agcaatgaat ggcgtacgcc gcacgcgtcc atagacgcgc gcgaatgaga ggaaacggtt
      601 cacggttaac gactcacgtt taacggactt catctccctc taacttcctc atattccgtc
      661 tcattccgac ccagcgctaa cgctagtttg tttgttctgt ttcaatcttt tttgtgttac
      721 ttagcattgt ttaacgcgta tcattcatat atttgcaatc ctaaaatagc tggtttgaaa
      781 cagtttttat cagttccatt tgcttgccta ctgcttgttg tggcggtgta tatatcgagc
      841 agctacgcct atccgcagga gggggctcgt acactagccg gtaaacactc ccagagcgat
      901 tcgctaaggg gtaggaatgt cccttttgat aagggtagtg caaggggtgt aggcaggtca
      961 gctactgata cgctaaaacc caatgagcgc aacatttacg gttaccttgg cattcaagac
     1021 cctaagagca agtcgtttgc ctggtatttt gaccgggaaa cctatgattt ggttcaaaca
     1081 caagcctttg acagctcgct atttttggtg cacctgatac tacccgggca aaagagccta
     1141 tcgccgttaa cctacctggg caacatgggc tcgcctgtgc agagtgacca tttttttgaa
     1201 cggccggtta gctatccgtt cctgttttca aagggctatt ccagctacga gcagccggtg
     1261 ctggatagga agcagtacaa cgtacgacgc ccgcatacgc tgctggagta ttcaactgcc
     1321 ggcaaaaggc gtaatgctga gcaaaacctt agggtttttc atacgcagaa tgtgaacagg
     1381 tacctgaact tcgggttgca gtacgactac ttcagcacca agggtatata cgaaaggcag
     1441 ctaacccgta ataacgattt tactgccttt gcaagctact ataggcatag ggtatcggca
     1501 cagggaacgt ttgcctacac atacattcgg aatcaggaaa atggcgggct ggaggacgat
     1561 aggttcatac aggataccgt tatggagccc aacctggtgc ctttcaggct taaggatgca
     1621 tcggttgagt accgtaaaag gagcttttcc ggagtggtgg gttacgatat tattacaaaa
     1681 agggtaaacg atagccttga aaatctgaaa tcgattctca ccgccaagct gatacttgat
     1741 gccaaccgct acacacgggt ttacgccgat accgaaaccg attcatcgta ttacggcaac
     1801 ttctatatca gcaccacatc tacccacgac tcggtgtacc tagccaccta ccagtcgacc
     1861 ctgcttgtgg agctaagcca aattgccaaa tatccgggca tacccggatt acgggcatgg
     1921 gtatcgggcc taatggggag ctactacaac tttacacccg ataactttat atatacctac
     1981 agcaatacca agctcaacac cagccacttt ggcctggggg tttacagcca aagcccttac
     2041 ctatcgtaca gtggcgctgc acgtttttac atgagcgggt acagggcagc cgataaggag
     2101 ttgcttggcc gtttatccat atcgccatgg aaatcgaagg agctgcctta cgtagcaggg
     2161 gagctaagca ttaccgatag ggaacccgat atctttataa atcgatattt ttcaaatcat
     2221 tacaaatggg acaacacttt tgaaaaggag agccgtttca ggttaagcgc acgccttgga
     2281 gccgataggt ttggttttga ggcagggtat agcattgagc atatccttaa ctatgtttac
     2341 tttgacacgc tctcgttacc tgcccaggaa cctaaggtta cggtaacctc ggcctacatt
     2401 cagaaaaaat tcaggctcgg ttggttcaac gctgtgtgta aggttatttg gcaggcaagc
     2461 accaatgccg atgttttaag cttacccacc cttgcgggat ttggtgcact ttacgtgcag
     2521 taccccgtgg taaaggatgt gcttaccatg caacttggtg ttagcggatt ctaccgtacc
     2581 gagttttacg ccgatgccta taatccagca cttggggttt accacaacca gcgcgttcga
     2641 aaaattggca actatccgtt tgtcgatgta ttcctgaatg cccggtggaa acgtgccaat
     2701 ttatttatta agcttgacca tgctaaccag ggattaattg ataatcagta ttttacaacg
     2761 ctacactacc cgtacaatcc acgtatggtt aagtttggag tttcgtggat gttttacgac
     2821 tagtcgtcaa aattctcaat tgtaattcaa cactttaggc taattttgtg tttctgtttg
     2881 gttaaaccaa aaaagtcata ttatggttcg aaaagttatt tacctgcgta atgggttgat
     2941 ttttttggct atgctaattt ttttctcggt cagtagctgt gagaaaaaag gggttaagga
     3001 cgaggaagtt cagcgagacc ttgccgatat actggccgat ggcaaacttg ttgctgtaac
     3061 cgattataac gcaacaagtt actttatcta caagggtgaa ccaatgggtt accagtacga
     3121 tatgcttcgc gatttagcta cctatttagg ggtaaagctc gaaatcgtga cagagaacga
     3181 tatcgacaaa tcgtttgaga tgctcaggaa gggcaaggtt gatatcatag ccagcagctt
     3241 agccataaca gcccagcgca aggaggaggt tgattttacc ttgcctcatg gcgaaaccag
     3301 ccaggtgctg gttcaaagga tacccgctga aaacggcact cctgccattc aaaaaccgct
     3361 tgaactatcg ggtaggttgg tttacgtgca aaaaagctca gcaggcgcac agcgcctaaa
     3421 aaacctatcc gatgaaatag gtgggggaat aaccgttgta gagctcccca actacgatgc
     3481 cgacaagctg attgagctgg tagctacagg ggaaatcgat tatgcagttt gcgaccagtc
     3541 cgtagccctg gtaaaatcaa actactacaa gaatatcaac atagatattc aaataggatt
     3601 cccgcaaaaa acggcctggg ctgtaaggaa aacctcggtt gagctccgga aaaaagttga
     3661 tacctggctg gagggttacc taaaaacggc tcactaccgt aatattgagc taaagtattt
     3721 tgcccaggaa cgctttaaca ggcgcgccag cagcgattac tttttcattt acaccggcaa
     3781 gatttcgccg tgggacgagt actttaagaa gtacagccag ggcattgact gggactggcg
     3841 gcttttagcc tcgctggttt accaggagtc gcgcttcagg cacgatgtta cctcgcacag
     3901 gggcgcatcg gggttaatgc agatgatgcc ccaaaccgcc gagtatttcg gagttgatag
     3961 tacggtaggg cctgccaagc aaattgaggc cggcgtgagg tatatcaagt ggctggaaga
     4021 ccgatttgcc gcttacgata ttcccaagga cgaaatggta aagtttgtgc tagcatcgta
     4081 caatgcaggg ataggccata taattgacgc ccgaaacctt gcccagaaac atggtcgaaa
     4141 ccccaatgtt tgggacaata acgtggagta ctttatccgg aataagtccc agtttgtaaa
     4201 cgaccccgtt gttaggcacg gcaagctaaa gggggttgaa accacaaact acgtgaagga
     4261 gataatggaa aggtaccagc actacaagaa tatcatagga gagtaaagct ctcctttttt
     4321 ttgctgtaac ctgctttatt catacatttg cgcaaaattt aagatgaaaa tgagtaatcg
     4381 tagagttagg gtgcgttttg caccaagtcc taccggccct ttgcatattg gaggagtgcg
     4441 tacagcgcta ttcaactatt tttttgcccg ccagcacggt ggcgatttca ttctcaggat
     4501 tgaggatact gactcgcaac ggtttgtccc cggcgccgag gagtatatta acgaggcgct
     4561 gaactggtgc ggaataaaga tagatgaggg cgttcgtgag ggtggcccgc atgcgcccta
     4621 ccgccaaagc gaacgcaagc acatatacaa gcagtatgcc gataggcttg tggagagcgg
     4681 tcatgcatac tatgcctttg acacacccga ggtgttggac aaaatgcgcg ccgaggccga
     4741 agcccaaggc ggggctttta catataacta cgccattagg aatgggatgg agaattccct
     4801 ggcactacca gccgatgagg ttaagcgccg cattgagcgt ggcgaccaat gggttattcg
     4861 ttttaagatg cctgagaacg aggaggtggt gatgcacgac cttatccgcg gcgaggttag
     4921 ggttaacaca tcaacccttg acgacaaggt gctgtttaag agtagcgata tgctacccac
     4981 ctaccacctg gccaacgtaa ccgacgatta ccttatggag atttcgcacg ttatccgtgg
     5041 cgaggagtgg ctaccatcgt tgcccctgca cgtgctgctt taccgagccc tgggctggga
     5101 gaatgtgatg ccaaagtttg cacaccttcc gctactgcta aagccatcgg ggaatggcaa
     5161 gctgagcaaa cgcgatggcg ataaaatggg attcccggtt ttcccgctgc tctggaaaag
     5221 cgccgatggc gaaatatcgc gtggttaccg cgaggatggt tactttcccg aggcatttgt
     5281 gaacctgctt gcccttttgg gatggaatcc cggaaccgac caggagctgt tcagcatgga
     5341 tgagctcata aagctcttca gcattgagcg cgttaacaaa tcgggcgcac gtttcgaccc
     5401 ggaaaaggca aaatggttca accaccagta ccttatccgc aaatccgacg ccgaggttgc
     5461 tgagctattc cagcctgttc ttgaacagta caatatcgat gtaccattcg acaagctcac
     5521 gcgaattgtt gggctggtta aggagcgcgt taactttgtg catgagctat gggggcaaac
     5581 aagctatttc tttgaacgcc acctggagta cgaccagcaa gctgttgcca agcactggaa
     5641 gcccgaaaca cccgatatga taaccgatgt tagcgaaacg ctaaaagcat tggatacctg
     5701 gaactcgcac gagattgaaa cagcgcttaa agaccttatc aacgctaagg gttacggcat
     5761 gggcaaggtt atgaacgctg taaggctttg ccttgttggc gaatcgaaag gccctgggat
     5821 tgccgatatt tgcgaaattc tgggcaggga tgaaaccctt acaaggctta accaagccat
     5881 tttaattctg aatggtaaga agtgaatcgg aatgaatcgg aatgaatcgg aatgaatcgg
     5941 aatgaatcgg aatgaggcgg aatgaggcgg aatgaggcgg aatgattcgg aatgactcgg
     6001 aatatgagga agttagaaga agttagaaga agttagaaga agtaagagga agttagagga
     6061 aatattagtt cgttaaacgt gatccgtgaa acgcaatgtt tgaggcggga atgagtcgga
     6121 atgagtcgga ataaacagaa tatgatggag ttagaggaag ttagaggaat agtaaaatgt
     6181 gaacggctga accaaatgac cttgaattaa caaatttaac cgggctaaat aataggaacg
     6241 cataattatt tgaggagatt agccagaaga tgcacaaaaa acatttaatt attgtttcag
     6301 ttgacgaatt cggatttaat tatctttgtg gtatgagaaa cattgtaagc atagctgcaa
     6361 cctgttgttg tatgtggtat aggtttgtac cgcaggggaa ttgctatgcg cgatgttgat
     6421 tactagttaa ctcaatccat aactcaagcc cttccggtac atacacggag gggctttttc
     6481 attttatatg gcttttagct actaaaacga taaacagatt gattttatgg aaaggaacaa
     6541 acttaactac attgaaaccc gccgaatggt tgaggactta agcgatagcc ttacagagct
     6601 ggttgttgag agtttgcttg gtgccggaca gacggttgat acagaaaagc cttacagtaa
     6661 actttacgag gcgttacggt attttaatcc cgataccagt caatgccaaa ggcaattgga
     6721 aattgtttac caaggattcc aaggtgtcct tgatacactt agccacgatg caaactttat
     6781 ctacctgaac gacccagcag cacaaagcct tgaggaggta ctgcttgcat accccggatt
     6841 ttttgccatt gcccattacc gccttgcaaa cctactgcat agggtagggg taaacatttt
     6901 gccaaggatg attaccgagt acgcacatag taaaacaggc attgatattc atccggcagc
     6961 aaccattggg caggagttct gtatcgacca tggaacagga gtagtgattg gcgaaacaac
     7021 cataataggc cagcgggtaa agctgtacca aggcgtaacc ctaggcgcgc taagcgtatc
     7081 gaagagcctt tcggccacaa aacgccatcc caccattgag gacgatgtga ttatttacgc
     7141 aggcagcacc atacttgggg gcgagaccgt gattggacat gatagcgtaa ttggcgggaa
     7201 tgtatggctt atcaaaagcg ttccgccctt ctcaatggtt taccatgaga gcaaagtgat
     7261 tgtacaacag ggaaagttta accataaaat gaagtgatag atgaaatact tgagtatcga
     7321 taaatcaatt ggagcaaccc cacacattag gcttagcagg cttttcccaa acgctgaggt
     7381 ttggcttaag gatgagcggc gcaatccgag cggtagtatc aaggataggg ctgccatggc
     7441 aatggttgag gatgccgaaa ggaaaggact tttaaaaccg ggtagtgtta tagttgagcc
     7501 aacatcgggt aataccggga ttggccttgc catggttgcg gctgtaaaag gctataggtt
     7561 gatactgacc atgcctgaga gcatgtcggt tgagcgaagg gtaatactaa aagcttacgg
     7621 agccgagatt atactaacgc cagcagcgca gggtatgcag ggcgcaattg ataaagcaaa
     7681 ggagtttatg gctcaactgc catcggcatg gatgcccatg cagtttgcca accctgccaa
     7741 tccaaacaca cactcactta ccacagccaa ggagatttta gccgattttc cggatggact
     7801 cgatatggtt gtggccgggg tgggtactgc cgggcatatc accggcgttg gtcgaacact
     7861 taaagaagca ttccccaaca ttcaggtaat tgcagttgag ccgttcgact cgcctgttct
     7921 ctccggtggg caacgggctc cgcaccccat tcaggggatt ggggctggct ttgtgcccca
     7981 aaacctcgac cgtggtgtga tagatgaaat aattacggta aaagccgatg aggcgattga
     8041 atgcatgcgg ttacttgccc gaaccgaagg gattttagct gggatatcaa ccggagcaaa
     8101 tattgctgcc gttaaaaaac tgctgtcagt aaagcagtcc aacctaagaa ttttaacctt
     8161 tgcctacgat actggtgagc gctacctgag tgtaaagggt atttgggatt aggtttaaag
     8221 cccggtaagg cgtttaatct cattcagctt attcagcgcc tcaatgggag taaggttgtt
     8281 gatatcaatc tttttaatct ggtcgcgtat ttgctttagt acggggtcct cgagctggaa
     8341 gatgcttagc tggtagccct cacggtgggt gccaagctca tcaaccggtt tgcttagcga
     8401 ttggcgttgg gtggaactct ccagctcagc aagtatctcg ttggcgcgtt ttaccacgct
     8461 cgggggcata ccggccatac gggctacgtg tattccaaag ctatgctcgc tgccacccct
     8521 aaccagcttc cgcaggaaga taacctggtt gttgacctct tttatggtta cattgtagtt
     8581 tttaacacgt gggaacgatt tttccatctc gttcagctcg tggtagtgag tggcaaagag
     8641 ggtttttgcc cttgcgctgg ggtgttcatg caggtactca acaattgccc aggcaattga
     8701 aatgccatcg taggttgatg ttccacgacc aatctcatcg agtagcacca ggctccgggg
     8761 tgagatgttg ttcaggatgc tggcagcctc ctgcatctcc accataaaag tcgattcgcc
     8821 aagcgacagg ttatccgatg ccccaacacg agtgaatatc ttatcgacta caccaattga
     8881 ggccgcttgg gcgggaacaa acgagcccat ttgtgccata agcacaatta aggcggtttg
     8941 ccttagcaat gccgattttc cggccatgtt gggtccagta attatgataa tttgctgctc
     9001 gcgtgtgtcg aggtaaacat cgttggctat gtattgctcg cctgccggca gcatgcgctc
     9061 aataaccgga tgtcgtccct gcctaatatc aattacatcg tcgaggttaa cctgggggcg
     9121 gttgtactgg aactcaactg cgcagtgggc aaatgatagc aggcagtcga gctgagctat
     9181 gaggtatgcg ttaagctgta tggagcccac atactcggca aggctgtgaa ccaggttctg
     9241 gtaaaggttt tgctccagct cggaaatctt ttcctcagcg ccaagaattt tctcctcata
     9301 ctcctttagc tcctgggtaa tgtagcgctc cgccgataca agggtctgtt tcctaatcca
     9361 atccgctgga actttgtcct tgtgggtgtt cctaacctca atgtagtagc caaaaacgtt
     9421 gttgaagtta accttaagcg atggtattcc cgttcgctcc gattcgcgct gctggatatt
     9481 taagaggtac tccttgccgg agtaaagtat atcgcgtaac tcgtcgagct cagcgttaac
     9541 accttttgca atcacctgac cctttccaag catcatggga gcatcgggga caatttcctg
     9601 ttcaatgcgc tcgcgtatgc ggtggcaggg gttcagctgc tccgatatgc gaagcagcgt
     9661 gggttcagcg ctttggtcgc agatggtttt aacctcatca atcaggctta aggcattttt
     9721 tagctgtacc atctcgcggg gctgaatacg acccaccgca gcctttgaaa tgattcgttc
     9781 cacatcgccc atatccctta tcttgtcggt aagggtagcg cgcatatcct cgttgttaac
     9841 aaatagctca acaacgttta gcctatcgtt aatggagtcg gtatttttta ggggtaaggc
     9901 tacccaacgc ttcagcaggc gggcacccat gggcgatgag gttttatcga taacctcaat
     9961 gaaagttttg gctccctcgt taatggagct gaacagctcc aggttgcgcg aggtaaacct
    10021 gtcaatccat acaaagctat cctcgtcgat acgggaaatg ctctgaatgt gtcctatttg
    10081 tgtgtgttgg gtaacatcaa ggtagtagag tatggcgcca gcagcggtaa ttccaaactg
    10141 caacgattcg acaccaaacc cttttagtga ttgggtttta aagtgttttg tcagtttttc
    10201 gcgagcagca tcctcgttaa aggcccactc gtcgagccgt gttacataat gtttgcttcc
    10261 aaactgctcg aggaactcct tgtactttgt tcgttcaaca agtatctcct tgggcttaaa
    10321 gttgttgata agcttatcga catagtccaa atttccctcg gaagtgtaaa actcaccggt
    10381 ggatatatca aggaaagcca ctcctcccgc ttttttatcg agatagacac aagccaggaa
    10441 attgttctcc ttacgctcca gaacattatc gttgtacgaa acgccggggg taaccagttc
    10501 agtaattccc ctttttacta tggttttggc ttttttgggg tcctcgagct gttcgcaaat
    10561 tgccaccttt tggccggcac gtacaagctt tggcagatag gtgtcaaggg catggtgtgg
    10621 aaatccggca agttcaacaa acgatgcagc accgtttgcc cggcgggtta aggttatccc
    10681 taaaatctcc gaggttttaa tggcatcctc accaaatgtc tcatagaaat cgccaaccct
    10741 gaaaagcagt atggcatcgg ggtggttacg tttgatgctc aagtactgct tcatcagcgg
    10801 agtttccaca atatttatat cattctcctt gctcaccgtt tattagtttt ccggaacgtt
    10861 tacaaaaata ccaaatagtt gcatagcaat cctgtcctgt tgaaaaaaac gttaaaacta
    10921 ctttacaaca taaaattgcc aggaatgagg attaaacttt tggctagttt tgcgctcaaa
    10981 taaagcgaaa cccccgactt cacagccggg gatttcaaat ggttcaggta ttagtaagga
    11041 ttggaaataa tcgcagtaaa gttaaaaatt ttcaacaaca ttttcaacac ttaggtttca
    11101 gtagaggaac gctgtgattg cttactaata cctaaccata tacaggacag gcttttggcc
    11161 tgtctttttt tatttagatt ttttttaggt tttttagacc ctaccgttcg atattagatc
    11221 ttaatccaat ttcgtttata aactcctttt gtcattctgt aaaacgaagg atatattgaa
    11281 tagtgtgcag tatatagtgt ttaggggtgt ttggagttta gtgtttagtg aaatgctttt
    11341 tttcactatc aaccatcaac tatcaactct gcttgtcatc ctgagcgcag cgaaggatct
    11401 ctgaccgacc taatgagatg tttcgctagc gctcacatga cattagtttc acgaacaaag
    11461 tttccagccc cgtaggggcg taatgtttgt aaccctatga ttactcccat gtgatgcgat
    11521 gcacgcaata ctttttccaa agaacagctc agaaaggttg catcgcaata aaaatgcatg
    11581 gcaaagagcg attgtttgaa aaccaatcaa aaggaataaa ggattaaagg cacaaagaga
    11641 tgcttcggcg agctcagcat gacaaagaga acgaggatgc cagaggtttg taattccgag
    11701 ctagtcgagg aatcacatta aaaagaaata aaggaggata tagatgcttc gactgggttc
    11761 agcatgacaa ataacattaa ctaaacaaca ttgaatatca ctcctcttcg aggaatttaa
    11821 tccaagtact atgaattttg aggaatgagt cggaatagac atgggtgatt ttatgaagga
    11881 atgaggcgga atagacggaa tatgttgatg tttgaggaat gttaaaatga acaccaaagc
    11941 caaaaatacc atgaattaac aaattcaatt gggcaaataa aaaaaggtga ggcggaaata
    12001 tttttagggg agattagtaa aagaaaaagg cgaccgttag ggtcgccttt ttttaagcat
    12061 cgatatagat tacttgtttt tcttatcctc tttacgtttc tctagatcgt aagcaattgg
    12121 tgctgctaca aaaattgagg agtaggtacc aattagaata ccaacgataa gagcaaaaac
    12181 aaaaccacga attacttcac cgccaaagaa gaacattggg ataagtacaa tgagagtagt
    12241 gaacgatgtt gagaaggtac ggctcaatgt atcgttaata gcgctattca tgttctccct
    12301 tctggtgcgc ttggggtgca gacccatgta ctcacggata cggtcgaaaa taataaccgt
    12361 gtcgttaatg gagtaaccaa taatggttag gatagctgca ataaacgatt ggtcaatctc
    12421 catattaaat ggcaccctgt agtaaaggat tgagaacaat ccaatggtta ttagcgagtc
    12481 gtggaaaaga gctataacac cgccggcact gtagctccag tacctgaacc tgataaggat
    12541 gtaaacaaaa attactacaa gggcaaagaa cacagcaatg taagcatcgc gtttgatatc
    12601 gtgggcaatg gtaggcccaa tcttaatgga gctttgcttg taatcgtgct ggaagtcgct
    12661 aaagttagcg ccattggcca taaacggttt aacaccgagg taaatcaatg aatcaacctc
    12721 gttgtctaca ttggggtcgt tatcggctac tttgtacttg gtggtaattt ttacctggtt
    12781 cgatgcgcca atggttttta cttcgggggc ttcgccaagg tatgttttaa gcgatttgct
    12841 tacctcctgg gttgaaacgg tattctggaa gcgaacaatg tatgaacatc caccggcaaa
    12901 gtcgataccc gggttcaaac cacgaacggc caacgaaata attgcaatgg caatcaaagt
    12961 gccggagaag aaataagcct ttttacgtag cccaaagaaa tcgattttaa ctttctggaa
    13021 agcattttcc gaaagtttgg tagagaaaat cattggaatg ttgcgttttt ccatccaaag
    13081 caggaatagc cttgtaatga atattccgca gaacatcgat gtgataatac caatcatcag
    13141 ggtagtagca aaaccacgga taggtcctga accaaacagg tataggataa caccggttag
    13201 cagtgtggta acgttaccgt cgataatagc ggagagcgca ttgcggtaac cgtccttaac
    13261 ggcaaggctc aaacctttac caccgcggag ttcctcacgg atacgctcaa aaataagtac
    13321 gttggcgtca acagccatac caagggtaag cacaatacct gcaataccgg gtagggttag
    13381 ggtagcacca aacgatgcca taacgcccat gatgaagaac aggttggtta caagggcaaa
    13441 gtcggctact aaaccagcgc tacggctgta gtaaataacc atgtaaagca gcacgatgat
    13501 gaacgacaca ataaaggagt tcattccaga gttaatggct tccttgccga gcgatggacc
    13561 aaccaattgt tcctgaacaa tatgagcagg agcaggaagt ttacccgact ttagcacgtt
    13621 ggcaaggtcc ttagcctcgt ttatggtgaa atcgccagta atttgtgaac gtccaccctt
    13681 aatttcggtt tgaacacgag ggaatgagta aacgtaatcg tcgagcacaa tggcaatgca
    13741 acgaccaacg ttttcgccgg tcatacgtgc ccaaatacgt gcaccctcgg cattcatttc
    13801 catgtcaacc tcggcttcgc ccttgcgatc gccaaaagct tcgcgggcat cggtaatggc
    13861 tccaccatcg aggggtgctt tgccatcgcg ggtggttact ttaagagcaa taagctcata
    13921 gaatacctta ttcttgtccc acttgggcga tttaactccc cagcggaatt ttacatcggg
    13981 agggaataat gccctaatct tgggcattct gagaatttca tttacctggg cagtgtcctt
    14041 gatatgggca tatccaacaa cagggccagg tgcgggttga ttgttgtata ggttaggctg
    14101 caaaatgctg aataaggggt aatccttagc catgtcggct gcggccttta aggtatcggt
    14161 cttttcggtt tccttaagtt tgcttagcaa ttcatccttc tgctcagtgg tttcatcttt
    14221 tttagtggtt tcggtaacag ttggctgact tgccttgttg atttcggcaa gggtgctgtt
    14281 ggcagccata aagttgttga acaactcgct gtactcgtag gtttcccaaa actcaaggct
    14341 tgcagttccc tgtagaagtt tgcgaacacg ctcaggttcc ttaataccgg gtaattcaat
    14401 aagaatgagt cctgaattct cgaggcgttg aatattgggt tgtgcaacac caaacttgtc
    14461 gatacggtta cgaagaatgt tgaaggagtt atcaatggca ttttcggctt cctgcttcag
    14521 caccttgagt acatcctcgt ccgaagaatc gaagttaata cgaccccgta gttcaggggt
    14581 attgaaaatt gaagccaacc gagcatttgg agccacctgc ttaaaggctt tgccaaactt
    14641 atcgataaag tgctcgctac cgg
//
LOCUS       DAOOWJ010000067        14637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4148_length_14637_cov_7.088740, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000067 DAOOWJ010000000
VERSION     DAOOWJ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14637
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4148_length_14637_cov_7.088740"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..469)
                     /locus_tag="PLW22_07660"
     CDS             complement(5..469)
                     /locus_tag="PLW22_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOU74619.1"
                     /translation="MRLFCFSALLILFATTTQAQGQTPVSNYNKALADSLGADEYGMK
                     WYVMVMLKTGTKQIKSRDSLQWLYKGHIDAINEMTNSGTLIVTGPMGKNDKNFRSFYI
                     LNTSKTEEAQKLMNANPAVKFGLFDAEYFFWYGPAALPTYKRFQPMVEAKKP"
     gene            618..1763
                     /locus_tag="PLW22_07665"
     CDS             618..1763
                     /locus_tag="PLW22_07665"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOU74620.1"
                     /translation="MAQILLVLFYLLIPFVILHLCHKFPFVNKLGAVFIAYLVGLIVG
                     NIGILPEWAGGIQDTLTTITVPLAIPLLLFSSNLKQWKSMAGKAMVSLLIGITSVVAI
                     VIAGYFIFMGKGMADLWKVSGMLIGVYTGGTPNLASLKMMLNVDPDVYILTHTYDMII
                     STVFLAFLMTVGKKFFSLFLPAFKDDNMEEVEYTNGSDPFWGIFKREIFYPLLKAYGL
                     TILIFAIAGGLSLLVPKNSQVVVVILTITTLSIVASTFKKINAIEKTFESGMYLILIF
                     SVVVASMADISRFAGLTPGLFGYITLAVFGSLFLQVVLGKIFKVDADTTIITSTALIC
                     SPPFVPVVAAAINNRKVILSGISIGIIGYAVGNYLGFAIAELLKTIQ"
     gene            complement(1805..3154)
                     /locus_tag="PLW22_07670"
     CDS             complement(1805..3154)
                     /locus_tag="PLW22_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU74621.1"
                     /translation="MRKLILLAAILFTVTGLFAQQTSDDKKAEKVKSGWNVGGLPVVA
                     YDTDLGFEYGALVNLFHYGDGTIYPKYKHNVYAEVSRFTKGSGINRLFYDSKYVIPGL
                     RVTADLSYLTDKMYDFYGFNGYESVFNLDWADDESADYRSRAFYKYDRKLLRFTTDLQ
                     GKLKNEKFGWVAGIGLYDFKIDTVDVDKLNRGKDEADKLPYVGGGLYQRYINWGLIGE
                     DEKNGGFVTYLKVGAVYDTRDNEPNPMKGMWTEAVITYAPSFLGIGSKYQHAKLSIIH
                     RQYFTLVPERLSFVYRLGYQGTLFGTCPFYLQPNMTTIFLRGATNEGLGGAKSLRGII
                     RNRVVGDGIAYGNFEMRWKFARFHFINQNFYLSLNAFVDGGQVVRKVDVEVPNFIEVP
                     YSPSDYFSNDKEKLHTSWGLGLRVVMNQNFVIVVDHGRAMTKDDGLSGTYIGLNFLF"
     gene            complement(3305..4915)
                     /locus_tag="PLW22_07675"
     CDS             complement(3305..4915)
                     /locus_tag="PLW22_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOU74622.1"
                     /translation="MEQEKKKREFPHTYVIIFALIVFAAVLTWFVPGGEFAREVKNVN
                     GIDREVIVPGSFHQVDNQPQTWQVFTSIFQGMNRTYDIIFYILMIGGAFWLLNESKAL
                     DVAILSFLNFTKRIERFKPLKVIGVDNLVIALIMICFSFFGAVIGMSEETIAFVVIFV
                     PLAISMGYDSIVGISLCFLGAGLGFASALLNPFTIGIAQGLSGIQLFSGIEYRFVIWV
                     IINTIGIGYVIWYARRIKKNPKLSPVYEIDEYWRHKAAQDENGTKSKPGKSAWATFIG
                     LALVFAIFAYYYPVSHLVVGQSIISVPIIPIATVVWILVGILALRHSVQLFIVNILLF
                     TILFLIVGVMGYGWYIKEIATLFLVMGLLAGIAFGKNANDLARSFIAGAKDIMSAALV
                     VGLASGIVVILEQGRIIDSLLNYSAEAMMGYGKVTSIAIMYVFYNLLNLIIASGSAKA
                     ALTIPLMSQFSDLIGISRQTTVTVYQLGGGFTNLITPTSGVMVGVLSIARIPYNKWFR
                     WFLPLMTILIITGFLLLLPTLFVPLSGF"
     gene            complement(4975..5700)
                     /locus_tag="PLW22_07680"
     CDS             complement(4975..5700)
                     /locus_tag="PLW22_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU74623.1"
                     /translation="MQNEELANKRNSEIFLEVFTEIERSLKEICKDEYTTNFAELLRK
                     ARGLNQVVNYYASDLKEFSQLRNAIVHTRRQNFIIAEPHPDVVQEVIHIKNLLQNPPV
                     VKTVMKLNPYRVSPSASVKEVLTTFAEKGFMRCPVVSNGKIVGLITAKTIARWLVNSS
                     GIIDNTTVSQLLNYIDNDDYCIVSENTDIVSLIALFNNSVRIGAYLQAALVTLNGKPD
                     SLLVGIITPSDFPSIIGQLKVKH"
     gene            complement(5682..6602)
                     /gene="miaA"
                     /locus_tag="PLW22_07685"
     CDS             complement(5682..6602)
                     /gene="miaA"
                     /locus_tag="PLW22_07685"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU74624.1"
                     /translation="MSVPNKYLIALLGPTGVGKTELSLKLAKHFNSPIVSCDSRQFYR
                     EMRIGTAFPTDWELAQAKHYLVGHKSVTERYSCGMFELDALDSLKQIYLSSDFAILVG
                     GSGLYGDALLKGIDDFPTPDPELRKLLQQQLKTDGIESLRHQLKILDPEYYKQVDLKN
                     PQRVLKAVEVCLQTGRTFSSFLTAPKKERPFKPIKIGLTRPREKLYERINKRVDQMIE
                     QGLVDEVKQLVGFRNYNALNTVGYKEIFQYLDGEITLDQAIELIKRNTRRYAKRQLTW
                     WARDNEICWFNPENQTEIIEYIERLCRTKN"
     gene            complement(6639..7172)
                     /locus_tag="PLW22_07690"
     CDS             complement(6639..7172)
                     /locus_tag="PLW22_07690"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74625.1"
                     /translation="MIYKFRMLSDEDKLFLRDFEVDADSTFYAFHKAIQENLGFDPGQ
                     LASFFLADEQWNKGMELTLIDMQNDTGMAAIPMERVKIKELMSNRRERLLYVYDIFAD
                     RNLFIELIDIQEPNPDVKYPVCTASVGDAPIQLAEDFAFEENISMEEPNEIDELFDEL
                     NDDDELGSLGLDDKDEW"
     gene            complement(7243..>8271)
                     /locus_tag="PLW22_07695"
     CDS             complement(7243..>8271)
                     /locus_tag="PLW22_07695"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOU74626.1"
                     /translation="KRINAFSKLGQQLPNAVRDPSHPLGHAAANAQHVNPWFTPENVL
                     NAVDAIAYEWLQPIELESWIKAYPEKYFTPPTAKTVGVVMAGNIPLVGFHDFLCVLMA
                     GHKINIKLSSKDGDLMGSVAKMLVAIEPAFADCIQITEGPIRDFDAVIATGSDSSVRY
                     FDFYFRNYPSLIRGHRNGVALLSGNETDDELALLADDIFTYFGLGCRNVSKLIVPEGY
                     NFNRLIGSMSRWKHLINHHRYANNYEYNRTILLMNQSPHLDAGFVLVVPNENIDAPVA
                     MVNYQEYSGINSAIDYINVNDSKIQCVVTKTNIDHSRRVIFGDSQKPKLNDYSDGIDT
                     IEFLGTLG"
     assembly_gap    8272..8371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8561..9811
                     /locus_tag="PLW22_07700"
     CDS             8561..9811
                     /locus_tag="PLW22_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU74627.1"
                     /translation="MKRILTLYGEIFRISMNSILATKLRSILTILIISLGIMALVGIL
                     TAIESIEGSLTNSFTSMGANTFTIQSRGTNVNIAGNRYRKKNYSYISLQQARQFKEDY
                     RFPANVSISVYASGASTIKYMSKKTNPNITVRGVDEEFMKVPGVEIERGRNFSSIDIL
                     DSRHVAIVGQEVANKLFMKNEDPIDKFINVGSGKYRVIGVLKNKGSGFGGGPNRTVYL
                     PYTNVAAFFSRPNMNYSISIQPYDPKLLEAAVSEAEGTFRIVRNLSATDESDFNIEKS
                     DNLAKLLIENLKFVSLAATVIGIITLIGAAVGLMNIMLVAVAERTREIGTRKAIGAKS
                     SEIKQQFLFEAILICQLGGLFGVFLGILIGNLVSVLTNGPFIVPWGWILGGLVISFVV
                     GLASGYFPAVKASKLDPIEALRYE"
     gene            complement(10978..11985)
                     /gene="gap"
                     /locus_tag="PLW22_07705"
     CDS             complement(10978..11985)
                     /gene="gap"
                     /locus_tag="PLW22_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU74628.1"
                     /translation="MTKIKVAINGFGRIGRNVFKIALERPELEVVGINDLTDTKTLAH
                     LLKYDSTQGRYEGKVEFDAENLIVNGKKYRVTAERSPLNIKWSETPDVVIESTGIFTK
                     RESEKGGYGDHIKNGAKKVILSVPAKDEIDRMIVLGVNDEALQPTDQCVSNASCTTNC
                     LAPVVKVLNDAFGVERGFMNTIHSYTNDQRILDAPHSDLRRARSAAVSQIPTTTGAAK
                     AVGKVIPELKGKLDGLAVRVPTPTGSLVDFVAVLKKEVTKEEVNAAMKKAAEGPMKGI
                     LEYTEDPIVSVDIIHNPHSSIFDAQSTMVNGNMVKVLSWYDNEWGYSNRVVDLIHKLF
                     K"
     gene            complement(12138..12716)
                     /locus_tag="PLW22_07710"
     CDS             complement(12138..12716)
                     /locus_tag="PLW22_07710"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOU74629.1"
                     /translation="MQKVTLAILAGGKSSRLGGIDKALLTVDGIPLISRIYNTFRTEV
                     WEVIVISNSSKTYPIDAPIFPDIILNCGPLGGIHSALTNASSSLVFIVSVDLPFASKD
                     IFQQLLCIYQKNRAEIVVPKHGDLMEPLFGLYNKTLLSRVNSILADRQGHPISELISQ
                     SNTKYLELPDDFQTRMWFYNINTHEDLKALEL"
     gene            complement(12723..>13241)
                     /locus_tag="PLW22_07715"
     CDS             complement(12723..>13241)
                     /locus_tag="PLW22_07715"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOU74630.1"
                     /translation="TDTVYGIGCDITNVKAVERVIKLKGLKPKDAHFSFICKDLSNIA
                     DFAKVSNPTFKLMKKNLPGPFTFVLPGLHRVLDYFLSKRKTVGIRIPNHQIPLELVDR
                     LGNPILTTSLKDSDEVVEYTTDPELIYENYKHLVDVVIDGGVGGNIPSTIVDCSGDEP
                     IILREGLGELIF"
     assembly_gap    13242..13341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13342..13673)
                     /locus_tag="PLW22_07720"
     CDS             complement(<13342..13673)
                     /locus_tag="PLW22_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOU74631.1"
                     /translation="MKFFIDTANLDQIREANDLGVLDGVTTNPSLMAKENIRGAENIK
                     KHYVDICNIVKGDVSAEVIATDYEGMIREGKELAALHPQIVVKVPCTKDGIKAIKYFT
                     DNGIRTNCT"
     gene            14147..>14637
                     /locus_tag="PLW22_07725"
     CDS             14147..>14637
                     /locus_tag="PLW22_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU74632.1"
                     /translation="MANTIKTIIVDDEPNSRDIIDFLLRTSKDVQVLDKCDNITQAFE
                     SIKHHNPDIVFLDIEMPGGSGFDLLHMLRNTPYNPTIVFITAFNQFAIKAIKHAAFDY
                     LLKPIDINDINDTLDRYREKQRNGIKVDKSKAELLLEHLEEFPKLKFNMRNGVLFVDP
                     DDI"
BASE COUNT         4190 a         3176 c         2839 g         4232 t
ORIGIN      
        1 taccttaagg ctttttagcc tcaaccatgg gctgaaaacg cttgtatgta ggcaaagcag
       61 cggggccata ccaaaagaaa tactccgcat cgaacaatcc gaattttact gcaggattgg
      121 cattcatcaa tttttgagcc tcttctgttt tgcttgtgtt aagaatgtaa aaactcctga
      181 agtttttatc attcttaccc attggccctg taactatgag tgttccgctg tttgtcatct
      241 catttattgc atcaatatgg cccttgtata gccactggag cgagtcgcga ctctttatct
      301 gttttgtacc agtcttaagc ataaccatca cataccactt cataccatac tcatcggctc
      361 caagggagtc ggcaagggct ttgttgtaat tggaaacagg ggtttgaccc tgtgcctgag
      421 tagtggttgc aaaaagaatt agcaacgccg aaaagcaaaa caacctcatt ttctgcaact
      481 ttattcttat ctgtataaaa tatcaagcca agttaattaa aaaatgttaa ttatgcaacc
      541 tgaaagctcg tatttattta tcaacagttt tttaaaaaaa ttgctatact tacaaccggt
      601 tttaaaatca atttacaatg gctcaaattt tacttgtttt attctactta ttaattccgt
      661 ttgttattct acatctatgt cataagtttc cttttgtcaa caaattaggg gctgtattta
      721 ttgcatacct ggtggggtta atagtaggta acattggtat tttacccgaa tgggctggag
      781 gtattcaaga taccctaaca accataacag ttccactggc cattccattg ttgctcttct
      841 cctcaaacct gaagcaatgg aaaagcatgg caggtaaagc catggtatcg ctattaatcg
      901 gaataacatc ggtagtagca attgtaattg caggttactt tattttcatg ggcaaaggaa
      961 tggctgattt atggaaagta agcggaatgc ttattggcgt atatacagga ggtacaccca
     1021 acctagcatc gctcaaaatg atgctaaatg ttgatcccga tgtttacatc cttacccata
     1081 cctacgacat gataatttcc acagttttcc ttgcgttttt aatgactgtt gggaaaaagt
     1141 tcttctcgct attcctgcca gccttcaagg atgataacat ggaggaagtt gagtacacta
     1201 acggcagcga cccattctgg ggaattttta agcgtgaaat attttacccc ttgctaaaag
     1261 catacggatt aactattctt attttcgcca ttgctggtgg tttatcgctt ttggtgccta
     1321 aaaatagcca agttgtggtt gttatactta ccattaccac attaagtatt gttgcatcaa
     1381 cctttaaaaa gataaacgcc attgaaaaaa cctttgaatc ggggatgtac cttatactga
     1441 tatttagcgt ggttgtggcc tccatggccg atataagccg gtttgccggc ttaacacccg
     1501 ggttatttgg atacattacc cttgcagtat ttggttcgct tttccttcag gttgtactag
     1561 gaaaaatatt caaggttgat gccgatacca ccataatcac ctccactgct ctaatctgtt
     1621 ctcctccatt tgttccggtt gttgctgcag caattaacaa tcgaaaagta attctatcgg
     1681 ggataagtat tggaattatt ggctatgctg taggaaatta ccttgggttt gccatagccg
     1741 agcttctaaa aacaattcaa taaaaaagcc ggccttaggc cggctttaaa atttcagtta
     1801 gcagctagaa taaaaagtta agtccaatgt aggttcctga tagtccatcg tccttggtca
     1861 tagcgcgacc atggtcaact acaattacaa agttttggtt catcacaaca cgcaagccaa
     1921 gtccccaact ggtatgcaat ttctctttgt cattgctaaa gtaatccgat ggggagtatg
     1981 gaacctcaat aaaattgggt acctcaacat caaccttacg taccacctga ccaccatcta
     2041 caaaagcgtt aagcgatagg taaaagtttt ggttgatgaa gtgaaagcgg gcgaacttcc
     2101 aacgcatttc aaagttgcca taggcaattc catcgcccac aacccggtta cgaattatac
     2161 cacgcaagct tttagcacca ccaagcccct cgttggttgc gccccttaag aaaattgttg
     2221 tcatattggg ttgcaggtag aacgggcatg ttccgaaaag ggttccctga taacccagcc
     2281 tgtaaacaaa cgatagcctc tcaggaacta atgtgaaata ctggcggtga ataatactta
     2341 gctttgcatg ctggtactta cttccaatgc ctaaaaacga cggagcatag gttataaccg
     2401 cctcggtcca catccctttc atgggattgg gttcgttatc gcgagtatcg taaaccgcac
     2461 caactttcag gtaggtaaca aatccaccat tcttttcatc ttcaccaatc aatccccagt
     2521 taatgtagcg ctggtaaagt ccacctccta catagggtaa cttatcggct tcatcttttc
     2581 ccctattcag cttgtcaaca tcaaccgtat caattttaaa atcatacagg cctataccgg
     2641 caacccagcc aaacttttcg ttttttagtt tcccttgcaa gtcagtagta aacctcaaca
     2701 gtttcctatc gtacttatag aatgcacggc tgcggtaatc ggcgctttcg tcatcggccc
     2761 agtccaaatt gaataccgat tcgtaaccgt taaaaccata aaagtcgtac atcttatcag
     2821 ttaaatacga aaggtcagca gttaccctga gcccgggaat aacatacttt gaatcgtaaa
     2881 acaatcggtt tataccacta ccctttgtga agcgcgaaac ctcagcgtaa acattatgct
     2941 tgtacttggg atagattgta ccatcgccgt agtggaacag gtttaccaat gctccatact
     3001 caaaacccag gtcggtatcg taggctacca cgggtaaccc tccaacattc caacccgatt
     3061 ttactttttc agcttttttg tcatcgctgg tttgctgggc aaataatccc gtaacggtaa
     3121 acaatattgc agcaagtagt attaacttcc tcatggaatt ggttttatga ttttctcaaa
     3181 actaaggatt tttttaaaat aggtagatac tactaatcga atgaatagaa atatttatgc
     3241 tttgcagata ttaatgttct tgacacctgg ttcagaattt aacagtaatt gctggcctct
     3301 accactagaa cccgctaagt ggaacgaata gagttggtaa aagtagcagg aatccggtaa
     3361 taataagtat tgtcattaag ggcaggaacc atctaaacca cttgttgtaa ggaatacgcg
     3421 caatgctaag aactccaacc atcaccccag aggttggggt aatcaggttt gtgaaaccac
     3481 ccccaagctg gtaaacggtt accgtagttt gacggcttat accaattaaa tcggaaaact
     3541 gcgacatcaa tgggatagta agtgcggcct tggccgaacc tgatgcaatt atgaggttca
     3601 acaggttgta aaacacatac ataatggcaa tggatgtaac tttaccgtaa cccatcatag
     3661 cctcggccga gtagttcagc agggaatcga ttatacggcc ttgctccaga attactacaa
     3721 ttccgcttgc caaaccaaca acaagcgctg ccgacataat atcctttgcc ccagcaataa
     3781 acgatcgcgc aaggtcgttg gcatttttac caaatgctat cccagccagc aaacccatca
     3841 ccaggaagag tgttgcaatc tccttaatat accagccata tcccatcaca ccaacaatta
     3901 aaaacagaat ggtaaacagt aagatgttta ctatgaaaag ctgaaccgaa tgcctcaatg
     3961 ctaaaattcc aactaaaatc catactactg tagcaatcgg aataattggg acagagatga
     4021 tactttgccc cacaaccaaa tggcttacag ggtagtagta agcaaatatg gcaaacacta
     4081 aagccaaacc aataaatgta gcccacgcac tcttaccagg ttttgatttg gttccgtttt
     4141 catcctgggc tgccttgtgc ctccagtact catcaatttc atatacgggc gatagctttg
     4201 gattcttttt aatccttcgg gcataccaaa tcacatagcc aatgcctata gtatttataa
     4261 tcacccatat aacaaaccta tactcaatac ctgagaacaa ctgtattccc gacaaaccct
     4321 gcgcaatacc aatggtgaaa gggtttagca aggcgcttgc aaagcctaaa cccgcaccca
     4381 ggaaacataa acttattccc acaatggaat cgtagcccat acttatggca agcggaacaa
     4441 aaatcaccac aaaggcaatg gtttcctcgc tcataccaat taccgcccca aaaaagctga
     4501 agcaaatcat aattagagct ataaccagat tatcaactcc aatcaccttt aagggtttaa
     4561 acctctcaat acgtttggta aaattcagga acgaaagaat agcaacatca agtgccttac
     4621 tctcgttgag caaccaaaat gccccaccaa tcatcaggat ataaaaaatg atatcgtagg
     4681 ttcgattcat tccttgaaaa atagatgtaa acacctgcca ggtttgaggt tgattatcaa
     4741 cctggtggaa ggaacccggg acaattacct cacggtcgat accattaacg ttctttacct
     4801 ccctggcaaa ttctccccct ggtacaaacc aggtcaaaac cgcagcaaaa acaataagag
     4861 caaaaataat tacataggta tgcgggaatt cacgcttttt cttttcctgt tccatgttca
     4921 tagtgttttg atgtaatacg gtttcaaggc taattgcccc taatccgtac aaaactaatg
     4981 tttaactttt aattgcccaa ttatagaagg gaaatcgctt ggggttataa ttccaaccag
     5041 taaactgtcg ggtttaccgt tcagcgttac taaagccgct tgcaggtaag cacctattcg
     5101 gactgaatta ttgaaaaggg ctattaacga tactatatcg gtgttttccg atactatgca
     5161 gtaatcgtca ttgtcgatat aatttaaaag ttgactaacc gttgtgttat caattattcc
     5221 agagctatta accaaccatc gcgcaatggt tttggcagtt ataagtccaa ctattttacc
     5281 gttactcaca accgggcatc gcatgaaacc cttttcggcg aaggtggtta aaacctcctt
     5341 aaccgaagca ctgggtgata ctctataggg atttaacttc attactgttt tcacaaccgg
     5401 cgggttttgc agcaggtttt tgatgtgaat aacttcctgc acaacatcgg gatgtggttc
     5461 ggctataatg aagttttgcc tacgcgtatg aacaatggca ttgcggagtt gtgaaaattc
     5521 ttttaaatcg gaagcatagt aattcacaac ctgattcaaa ccacgcgcct tgcgcagtag
     5581 ttcagcaaaa ttggttgtat attcgtcctt acaaatttcc tttaggcttc gttcaatctc
     5641 cgtaaaaact tcaaggaata tttcggaatt gcgcttattt gctagttctt cgttctgcat
     5701 aatctttcga tgtattcaat tatttcggtt tgattttcgg ggttaaacca acaaatctcg
     5761 ttatccctag cccaccatgt cagctgccgt ttggcatacc gacgggtatt ccgtttaata
     5821 agctctatag cctgatcaag ggttatttca ccgtcgaggt attgaaatat ctccttgtaa
     5881 ccaaccgtat taagggcatt ataatttcta aaacctacaa gttgctttac ttcatccact
     5941 aaaccctgct caatcatctg gtcaaccctt ttatttattc gctcatacaa cttttcgcgg
     6001 ggtcgggtta aaccaatttt tatgggtttg aatggtcgct cctttttggg tgcggttaag
     6061 aatgagctaa aggttctgcc tgtttgcagg caaacctcaa ctgcctttaa taccctttgc
     6121 ggattcttca aatcaacctg tttgtagtat tcagggtcaa gtattttaag ctgatgcctt
     6181 aagctctcaa tgccatcggt ttttagctgt tgctgtaaca attttcgtag ctcagggtca
     6241 ggtgttggaa agtcgtcgat gccttttagc agagcatcac cataaagccc ggagccgcct
     6301 acaagtattg caaaatcgct gctaaggtag atttgtttaa gcgagtcaag ggcatcgagc
     6361 tcaaacatgc cacagctgta acgttcagta accgacttgt gcccaacaag gtagtgtttt
     6421 gcttgagcga gttcccaatc ggtagggaaa gcagtcccta ttcgcatttc cctgtaaaac
     6481 tgtcgggaat cgcacgaaac gattggtgaa ttaaagtgtt tggccagctt aaggctcaat
     6541 tccgtttttc ccacaccggt tggtcccaaa agggcaatca ggtatttatt gggcacggac
     6601 attcatcaaa tccagttaaa tggatatagt ttgataaact accactcatc cttatcgtca
     6661 agacccaagc taccaagctc atcgtcatcg ttgagttcat cgaaaagctc atcaatctca
     6721 ttgggttctt ccatggagat attctcctca aaagcaaaat cctcagcaag ctgaataggc
     6781 gcatcgccaa ccgatgcggt gcaaacagga tactttacat caggattggg ttcttgtata
     6841 tcaatcagct ctatgaaaag gtttcggtca gcaaaaatat cgtaaacgta gagcaagcgc
     6901 tccctacgat tactcattag ctctttaatc ttgacccttt ccatgggtat agcagccatc
     6961 ccggtatcgt tctgcatgtc aattagtgta agctccatcc ccttgttcca ttgctcatcg
     7021 gccaaaaaga acgaggctag ctgaccaggg tcaaaaccta ggttttcctg aattgcctta
     7081 tggaaagcat aaaaagtact atctgcatct acctcaaaat cgcgcaggaa tagcttatcc
     7141 tcatccgata acattctaaa cttgtaaatc atatcaggta gtttatcaat acaaaaatag
     7201 catcaatatt aaacatttct aaaaatttca agggaaaaac ctttacccta atgttcctaa
     7261 aaactcaatg gtatctattc catcggagta gtcgttcagc ttaggttttt gggagtcgcc
     7321 aaatataacc ctccggctat ggtcaatatt ggtttttgtg accacacatt gaatttttga
     7381 atcgtttaca ttgatataat caattgctga atttattccc gaatactcct gatagttaac
     7441 catggcaacc ggagcatcga tattctcatt tggcacaact agcacaaaac ccgcatcgag
     7501 gtgtggactt tggttcatta acagtattgt tctgttatac tcgtagttat tggcatacct
     7561 atgatgattt ataagatgtt tccacctact catactacca attaacctat taaaattata
     7621 tccttcaggg acaattagtt tcgaaacatt tctacatccc aacccaaagt aggtaaaaat
     7681 atcatcagca agcaatgcta gttcatcatc tgtctcattg cccgacagca atgccactcc
     7741 attcctatgc ccgcgtatta acgaagggta gttccggaag taaaagtcaa agtaacgcac
     7801 tgagctatcg ctacctgtag ctataaccgc atcgaaatcc cttattgggc cttcggttat
     7861 ttggatacaa tcggcaaatg ctggttcaat ggcaaccagc attttagcaa ctgaacccat
     7921 caaatcccca tcctttgacg aaagcttgat gtttatttta tggcctgcca tgagaacaca
     7981 aaggaaatcg tgaaacccca caaggggaat attcccggcc ataaccaccc caaccgtttt
     8041 tgccgttggc ggggtaaaat atttttcggg gtaagcttta atccaactct ctagttcaat
     8101 gggttgaagc cactcatagg caattgcatc aaccgcatta agcacatttt caggggtaaa
     8161 ccaaggattt acatgctgtg catttgcagc agcatgaccc aacgggtgac tcgggtcacg
     8221 aactgcgttg ggcaattgtt gtccaagctt actgaacgca ttaatccttt tnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacgaactg cgttgggcaa ttgttgtcca
     8401 agcttactga acgcattaat ccttttgtct aatatcatgt cgaatgttat tggaaatgca
     8461 aacctaatcg attttttagt tcggtttgta agatttaatt tttagtaaat gaaaaagtgc
     8521 aaacttaaac ctaaatttga atttaaatta aaacagacac atgaaaagaa tcttaaccct
     8581 ttacggtgaa atttttcgaa tttcaatgaa ctcaatactt gccacaaaac tcagatccat
     8641 tcttaccatt ctaattatct cattaggtat aatggctttg gtgggcatcc taaccgcaat
     8701 tgaatcaatt gaaggaagtc ttaccaattc atttaccagt atgggtgcaa atacctttac
     8761 cattcagagt cgtggaacta atgtaaatat tgcgggaaat agatacagga agaaaaacta
     8821 ttcatacatc tccttacagc aagcacgtca gtttaaggag gattaccgat tccctgccaa
     8881 tgtatccatt tcggtttatg catcgggcgc gagcaccatt aagtacatgt ccaaaaaaac
     8941 taatcccaac attactgttc ggggtgttga tgaggagttt atgaaagtac cgggtgttga
     9001 aattgaacga ggccggaatt tctcatcaat cgacatactg gatagccgac acgtagctat
     9061 cgttggccaa gaggtggcta acaaactatt tatgaagaac gaagacccaa ttgataagtt
     9121 tatcaatgta gggagcggaa agtacagggt gattggtgtg ctcaaaaata agggttcagg
     9181 gtttggaggt gggccaaacc gtacggttta cctgccatac accaacgttg ccgcattctt
     9241 ttcgcgccca aacatgaact attcaataag catacaaccc tacgatccta aactgcttga
     9301 agctgcagta agcgaagccg aagggacttt tcgaattgta cggaatctct cggcaaccga
     9361 tgagagcgat tttaacatcg aaaaaagcga caatcttgcc aaacttctaa ttgagaacct
     9421 gaagtttgtt tcattagcag ccacagtcat tggcatcatt acgcttatag gcgcagcggt
     9481 tgggcttatg aacattatgc tggtagcggt ggcagagcgc acccgcgaaa taggcacccg
     9541 taaagcaatc ggtgcaaaat cgtcggagat taaacagcag ttcctctttg aggccattct
     9601 tatttgccag cttggtggtt tatttggcgt attccttgga attctaatag gtaaccttgt
     9661 ttcagtgctt accaatggcc cgttcatagt tccctggggt tggatattag gcggtttggt
     9721 tatatcgttt gtggtaggtt tggcctcggg ctatttccct gccgtgaagg cctccaagct
     9781 cgaccccatt gaagcgctta ggtatgaata gggcatagcc atttggaatg ccgcagaaac
     9841 catcattatg aattaaagat gttaaaacta tagtcttcat ttgaaaaatc aaatgataaa
     9901 tcaatgtcgt ttagttgagg atatttgtca tattgagtgg tagcaaaata cccctctgtc
     9961 atgctgagcg ctgccgaagt atctttttgt accttaaatc cgctaattac tttttgattg
    10021 gttttctggc aaccactcct tgttatgctt ttctgttgca atgcaacgtt tcagtattgc
    10081 tcgttggaat gtcatagcgt gcatcgctat acatgttgga gtagccatga agttgcaaat
    10141 atttagcccc tacggggctg gaaacgttgt tcgttgttcg tggaaaacga cctcaccccc
    10201 tcccctctcc tgacaggaga ggggtgaagc aattaggaga cctaattgca gggctaaagt
    10261 ctcccctctc aggggagatt tagaggggtc atctaggttg aatgtggaaa acaacctcac
    10321 ccaaccattt tccttaaggg gggaatggag cggaagaagc aagcagaggc ataaaagcca
    10381 gacccgacaa tgggtcgggg gtggaacgta gcggtgggct ttgtaagcga taccataaga
    10441 gtcacgctgc aggcgtggcg aattgtatcg cgtggcagtt ccactcccgg ggcacccatc
    10501 gggtcgggct tttcagcctt gattttcttt gattctttct tgtataaaga caagaaagaa
    10561 cgtgaaatgg ctctttgcag cgcctttcta ttgcgatgca actattcagt attgctcttt
    10621 tgagtatcat ggcgtgcatc gctatacatg tgggagtagc catggagtta caaatgtttc
    10681 gcccctacgg ggctgttcac ggaataactg aaagagattc ctcgactagc tcggaatgac
    10741 aataaccctt gtattcttac tccttttgtc atgctgagcc cagtcgaatc atctcttttc
    10801 tcttctacac ccacttgccg ttgcccgata tccttcgcta caacattatg acaaaaagag
    10861 gatctgtttt tttatctgaa cgatattgaa tgataaacat atgtcagaac tttacctatc
    10921 aactatcaat aaaaaaaggc tgcttgaagg cagccttttc taatcctaca acgcttatta
    10981 tttaaatagt ttatgaatta ggtcaaccac gcggttagag taaccccact cgttatcgta
    11041 ccatgaaaga actttaacca tgttgccatt aaccatggtg ctttgagcat cgaaaattga
    11101 agagtgaggg ttgtgaataa tgtcaaccga tacaattgga tcctcagtat actcaagaat
    11161 acccttcata gggccttcgg cagccttttt catagcagcg ttaacctctt ccttggtcac
    11221 ctcctttttt agcacagcta caaaatcgac aagagaaccg gttggggttg gaacgcgaac
    11281 tgcaagacca tcgagtttac ccttcagctc aggaataacc tttccaactg cttttgcagc
    11341 accggtagta gtgggaatct gtgaaacggc ggcagaacga gcacgacgca aatcgctgtg
    11401 gggggcatca agaatacgtt gatcgttggt gtaggagtga atggtgttca tgaaaccacg
    11461 ctcaacgcca aaagcatcgt tcaaaacctt tacaacgggg gccaagcagt tggttgtaca
    11521 gctggcattt gatacgcact gatcagttgg ctgtaaggct tcatcgttta caccaagtac
    11581 aatcatccta tcaatctcat cctttgcggg tacgctaaga ataacctttt ttgcaccatt
    11641 cttgatgtgg tcgccatagc cacccttctc gctttcgcgc ttggtaaaaa tacctgtcga
    11701 ttcgattacc acatcgggag tctcgctcca cttgatgtta aggggtgagc gctcagctgt
    11761 taccctgtac tttttaccgt ttacaattag gttttcggca tcaaactcta ccttaccctc
    11821 atagcgaccc tgggtggaat cgtactttaa caggtgggcg agagttttag tatcggttaa
    11881 atcgttgata cctacaactt caagttcggg acgctcaagc gcaatcttaa aaacgttgcg
    11941 gccaattcgt ccaaatccat tgattgcaac tttaattttt gtcatagcta gttgaagtta
    12001 gttattatga attgtgagtt taaaattcag cctaacaaag gtacttaaaa ttaaacaaat
    12061 tgttaatgta aaatttgaat attcaacaat ttttaacgca aacggttgag aaccaaaggt
    12121 tgttagcagg ttacaaatta tagctctaag gcctttaggt cctcgtgggt attaatatta
    12181 taaaaccaca tccgggtctg aaaatcatca ggtaactcta ggtactttgt gtttgactga
    12241 cttataagtt ctgatatagg gtgtccttgc ctgtcggcaa gaatactgtt tactctcgat
    12301 aaaagtgttt tgttatacaa gccaaacaag ggttccatta aatcgccatg tttggggaca
    12361 acaatctcag ccctgttttt ctggtaaatg catagcaact gctggaatat gtccttactg
    12421 gcaaatggta ggtcaacaga gacaataaat accaaactgc ttgaagcatt ggttaaagcg
    12481 ctatggatac cgccaagagg tccacaattc agtataatat caggaaaaat gggcgcatcg
    12541 atgggatagg tcttgctgct gtttgaaatt acaattactt cccaaacctc agttctaaaa
    12601 gtattgtaaa ttctacttat caagggaatc ccatcaactg ttaaaagagc tttatcgatt
    12661 ccccctaatc ggctcgattt gccgccagct aaaattgcga gtgtaacctt ttgcatgatg
    12721 acctagaaaa tcagttcgcc aagcccttcg cgcagtatta taggctcatc gcctgagcaa
    12781 tctacaatgg ttgaaggtat attgccacca actcccccat ctattacaac atctactaaa
    12841 tgcttgtaat tttcataaat gagctcagga tcggttgtgt actctacaac ctcatcgcta
    12901 tcctttaacg atgttgttag gatagggttg cctaatctat caaccaactc taatggaatt
    12961 tggtgattgg gtattcttat accaacagtt ttccgtttac tcaaaaagta atcgagaacc
    13021 ctgtgcaaac ccggtaaaac aaatgtaaaa ggaccgggca aattcttttt catcaactta
    13081 aatgtggggt tgctaacctt ggcaaagtcg gcaatattgc ttagatcttt acagataaaa
    13141 gagaaatggg catccttggg ctttaacccc ttgagcttga tgacacgttc aaccgccttt
    13201 acatttgtaa tatcgcaacc tataccgtaa accgtatcgg tnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn ngtacagttt gtgcgtattc cgttatcggt gaaatacttg
    13381 atggccttta ttccatcctt ggtacacgga acctttacta ctatttgcgg atgcaaggca
    13441 gccaactcct taccctcacg tatcatgccc tcgtaatcgg tggcaataac ctctgcgcta
    13501 acgtcgccct ttacaatgtt gcaaatgtca acgtaatgct ttttgatgtt ttccgcacca
    13561 cgaatattct cctttgccat caacgaaggg ttggtagtaa cgccatcgag aacaccaaga
    13621 tcatttgcct cacggatttg gtcaaggttt gctgtgtcaa taaaaaattt catacctttt
    13681 tgttttagct tgatttactg ccgcaaaact aatcaattta gtcggatgtg gcaatttaaa
    13741 atgggatttt agtagattca gaacttaaag aaaagccaag ggtggtgcgt gagccgtgaa
    13801 ccgtgaacgg cgaaacggaa tcgaacaact aaccacgctc ttgtcaatcc gagcatccta
    13861 tcccacttta gcggggttta agtttcaagg tttaatagct tgtccgctat cgactaccaa
    13921 cttggtaatt atcaatgtgt tacacagatt caacacggtg tttcacggtg tatgtcagcc
    13981 taatctgtgt catcagcgtt ccatttcaca tctcaatcaa cgaacagcta atccaaataa
    14041 atctgctcta atacttgctt atattccaaa aaggttttat atctttgata tatagagagt
    14101 aaaatttttg gataatgctc taaatacttg tgataaataa tatcaaatgg cgaatactat
    14161 aaagacaatt attgttgacg acgagcccaa ctcaagggac attattgatt tcctgcttag
    14221 aacaagcaag gatgttcagg tgttggataa atgcgataac attacccaag cctttgaatc
    14281 gattaagcat cacaaccccg atattgtatt tttggatatt gaaatgcccg gtggttcagg
    14341 ttttgacctc ttacatatgc ttaggaatac cccctacaac cctaccattg tctttattac
    14401 agcctttaac caatttgcca ttaaagccat aaaacatgcc gcattcgatt atctgcttaa
    14461 acccattgat attaatgata ttaacgacac tttagaccgt taccgcgaga aacaacgtaa
    14521 tggtataaaa gttgataagt ccaaagcaga gctccttctt gagcaccttg aggagtttcc
    14581 taaacttaag tttaacatgc gcaacggggt tctttttgtt gatcccgatg acattat
//
LOCUS       DAOOWJ010000068        14238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4298_length_14238_cov_8.710781, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000068 DAOOWJ010000000
VERSION     DAOOWJ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_14238_cov_8.710781"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLW22_07730"
     CDS             complement(<1..607)
                     /locus_tag="PLW22_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74633.1"
                     /translation="MKRFEKRISGLLIGSIFPLSLGLLSLTIWFLLDKSESRLFIYLI
                     SGLLIGGLIDIKFLKSWINQRFNLPIWIIASIYIMYNIFVYGFFMGLPVFNAFLGLLA
                     GYYFGNRICFNKIKPERQPKLLNQVSLFTGVIMTLVCISSGFLAIYDNGAAGMIQDVL
                     GLNFEVTRSMLWGIVLIGGFILIFVNVFLTRYTMIKTIKNYA"
     gene            complement(758..2866)
                     /locus_tag="PLW22_07735"
     CDS             complement(758..2866)
                     /locus_tag="PLW22_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74634.1"
                     /translation="MKIIQNKVRFIAILILLIPAKVLPQQIDNSGANFTSIKLIGRAT
                     SDSVVLRWAPTTPGGWLIANTVGYRVERLKVEEGEEITSFGYKSITPEAIKPMQLDAW
                     MNYAGVNNKFSAIAAQSLYGESFVPNPLKSDSLINLRNAADELTNRYSFALFAADNDL
                     ITANALGLRFVDREVIPNSRYIYRVTVVETVKEMAFDTAYLMVETQPAKALTSPVGFS
                     YRSGDGSIQLTWPNALNSEYIGYYLYRSDDGGKTFFAKNQMPIVYAQATNQVAVENIL
                     YIDTATVNYKVYSYRLVGITPFGELSPPNEIKAFSRDLTPPAAPIVKKSTQIAPGKIK
                     LTWEQKSQPADLKGFVISQSDNANYGYKFITAEPLPKNDREFVVDLGSDFEAYFMVAS
                     VDTAGNLGFSLPILANLTETPAPKAPQEVKGKIDKNGHVTLMWEPVNSKNIIGYRIYK
                     SNDPSHEFIAVNGQVHPDTVFIDSISLNTLSKNIYYRVAAVNSRYQYSDFSSVIKLNR
                     PDFTPPASAVIHDISVTDKSVTIQWHCSPSSDVAEQLLYRREESDSAWTIINTLKPGI
                     NRYVDLKVMPKTTYYYTLSNVDSSGNVSKMANAVSARPYDSGKREPVKQLTAQYDRQS
                     KSVILKWQYPDNATKKYWFVVYRANGDKDFVELKGLKPDALEFIDTSPLKGAYSYGIV
                     VMTSDGGQSEMVKQSIYVPE"
     gene            complement(2878..4524)
                     /locus_tag="PLW22_07740"
     CDS             complement(2878..4524)
                     /locus_tag="PLW22_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74635.1"
                     /translation="MKRFLKISITAWMVICFIMVSHAQEEEVKKSKIDVKTSATLASN
                     FYSSNGIDPRQPTNMQYGIIRTSVMLWDVVELPFELYFTTGQTRFQQPFNQFGVSPKI
                     SNWLTLHAGYFSTRFSDLTFGDMRLLGGGFELTPGKFRLKAIYGRSQKAVEPDKVSFA
                     PGVYRQNAYAVSLGYGNLSKSYFNINLFHAKDDSTSIKSDSVNVKPSENFVTSVDFGI
                     RFGEYVTLRGEGAVSVFSSNINSEKLDNVKVNIPELLFTPNASSRIDGAGLLSIDINP
                     SAYWSVSLDTRWVGPGFNTLGYTLMPNDWLEYSISPRLKLFGNKLILRSRIGVRYNNL
                     RDNRLATTSRFTGMLNATYQVTKIFGIDGNYSKNQIESGHKVDTLRISNVFDSYTLTP
                     RLSFKAFEGNNNVMLTFSYQNSSDKNVYTQNLNNVKTSGVNVIHIITLPSSLSLSTTL
                     LYNKTAMDLYTSTITHVSETVGKRFFKDRLNTSASLGVNWVEVTDKSTQLVFRVNASY
                     SLKIYGSLSFFITNNSYRGYGVVSQNYSEIYGNLQYNINF"
     gene            complement(4544..11503)
                     /locus_tag="PLW22_07745"
     CDS             complement(4544..11503)
                     /locus_tag="PLW22_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74636.1"
                     /translation="MSKFHISKKKYKIKLTISLILVFFSLNSLAQVDLKMILRNPLPS
                     EISEWVNDPSIFQIVITNMGNDEYPNCTAGFTITNEKGKVIASTKTGSRYLPRFTILK
                     PPSVLILNGPQVLNMNAVEYDRSIERIALNTNALPEGNYQLCVSVYDYRGNNITIGGE
                     YCMNIITQIPEPPTIISPSDNEIILSSIPIFRWMPLTNYNPRTVQPRYKIKVCPVFQG
                     QTPRTAIEVNPILFEKSDITSTFYQSIPGDLPFDFYPQVTQYAWIVQAFDPSGKPIGG
                     NQGRSEVATFRVGKDEQEFSIDNIYPVAGDTLPWAHPQLIAKISPFNSTVTQVALQLS
                     VREDGSATIYTYPKVMSYSPRQFESPITPELAGVVVCNYSSGAIAPFMAALKQGKKYY
                     WKVDATITTADGKTTTVNSRETSFAVGLSKAKSFKPAADTLMEIGNGVELSFEVNQPQ
                     QLNYPNYSLFNVPFFNGAYATTMATAKIKFEVSKKQSFDSLVYQSTITLPSTGHYSTG
                     SDCPELYQRINHRISQLSDTGKYYWRVKFLDPIDDAYFTSQPASFRIVADSLTSCFTM
                     NVDKPTNNGNWTENQTPTFAILIKPTIKKKHISGGRLRIWKMTSESQKLSDVIKEKPV
                     FDESFTGNENNKIAELSTDINGYTRWDLPFINGDSNSKEFKGDDNTYYTWNFRLNYNK
                     DSIRADGCKCKADSVISNQGVFQIKTSAKDENTCPGNCFAEAPTNTTPGTQTLAKDST
                     ITIGNFTMKLISVSGTPESLSGEGSIEVPYLRAPILVEFSNIGVNGDNKVYQGEVYAK
                     IDPAAPYSKADGNDFEGEVLNMADNQLKSIYEWSSSLGRLVSSLINTNPVTLPIGLDR
                     NIEGNNTVVAIIAMKFTPTQAVLNAATWAELPLLGPDVGFGFGAKNLCFHKDGLGGMK
                     RAELYLVHDFGYRSDESWSFLFKSPTPTDSGTYAKWDCKGLHYIEVKAEVEFPKTWLK
                     MANPDDSLQNVKAHFAGRAEKNGNSWGWILNANMDDCEFTHLPGFKMKVNSMVFDYTT
                     IQNPENITFPNGYTGDKTNTWKGFYIKNAEITLPDNLKTFDDANPKIQVSNLIIDGNG
                     ITGKLYAENLFQEFDGDFGGWGGSIDTLKAEIINNTLSNWRMRGRIKTSFTDTLFVYN
                     GLYNRSINQDNNTISNDYNLELVVNTEHGYEREIPLSWLPLKMKIDPATSIKVTYNKK
                     DVKAEANLNATLTFEGSNDILSKLDFKGIKVEGLTFRTYEPYVENGNWDFASPQHSMA
                     GFPVNIDGVSSVTRFSNGAFGPGVRFNLNIGLQPGSNAVNGTTTLTFWSKLINDRGRQ
                     RFMFDGIELDSIGVNADLGAVKVRGGLNLFQSDATYGDGYRGVVTASFIDNITTSATA
                     QFGMVNKFRYWYIDAKTILPTGLPVFPNFSMYGFGGGAWYKMHRSGTTNLAEQAFAPD
                     SSKNPSKTNSGFTYVPDNSASLGLSAQVIMGTSPSAETFNGDFTLQAQFQNGGGLSSV
                     SLLGNGYMLCSINERNKAKILADADINYTVSEQTLHGVFNARVDYQPLTGSGQMVMHF
                     APDLWYIKFGEPSKRMQLTFADWLRSDAYLMVGQQLPPPPALPAEIQQAFPGYTQSRS
                     FMIEQGNGIALGASTGFNTGRQQYMIFYGDVAAQAGYDMAMLKYNGVTCEGRSGELGI
                     NGWYATGQVYAYLNASIGMHVDVWFTSGNFEILSLNTAAMLEAGLPNPTWIKGAIGGN
                     YRILGGAVKGYCNYHFSMGDECQMLGDNPLARIDLISDISPVSGQNGVDVSIEAQVAL
                     NFKLNEPFQLKEMTNNGQTRIRTFRIKMDQFKIVNATNNDSIPGRLSIDPEGFSAYYK
                     PFEMLEGHTNFKVLASVYGEELVNGVWRPATNQNGQVIKQQVTATFKTGSRPDRITEQ
                     NVAYSYPVIGHKYFLQDECRAGRVQLKSGQSYLFQPREGYNVQFIARFMPTDIALMPV
                     DVPLTYNSSTNTLLFDIPNLINSKSYYIQFIRKEIPNDPNLQRMLDLGQQFLNSGGMQ
                     HSLTTNESNIYNRGGSQVNLVRRTINQNSNLNSGERLLYAVWFSTSRFNTLSQKLATF
                     NHTSTSVETNGNFERLTANYQGEECFDEFDFRPITWVSSGTVNRFGPLVKINAWQRNS
                     QWHNQFANPLVYDEIAWMQSNGYWNGPINYNQYMSDPSLSFVDIDFNTSMSVSVNAIG
                     QPFGIGGTGGTGQSSTGAVALVQGGFSTSNQPQNLSLFQSSSAPYFKLTYNHGIIVPT
                     DFMNLKYRAIATLSNPLITINAAKRNRLNAIINSNYQRMYRGTYPLSFYYDYFGCRVI
                     DTEPVTISKPFIY"
     gene            complement(12117..12695)
                     /locus_tag="PLW22_07750"
     CDS             complement(12117..12695)
                     /locus_tag="PLW22_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74637.1"
                     /translation="MKLINQDKIIALLFLALTILFLFVLMTNQTFFDWVFDRHHNQLS
                     WYIRPIFLIPFCFFAYKRNWTGISITVFCLFTSMFWFNKPEFVSDNVKTFLQFEKDWL
                     YSQWDYKKIILIITVPVSFFALGLAFWKRSLLMGLGVVVLMATGKIVWSIQNAGESGK
                     SIIIPAILGLLICSGLIYYGFIKLEKKKTTKR"
     gene            complement(13177..14112)
                     /locus_tag="PLW22_07755"
     CDS             complement(13177..14112)
                     /locus_tag="PLW22_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU74638.1"
                     /translation="MNRFKIDILFTIFVCLVFQFSCSNNKPISYDPPEGANYIGETVE
                     ITLDCGDILVGTMTIPKDEIKEFPCVLLITGSSPHDRDNSKPDNPITAYRPFRQIADL
                     LSTKGIAVLRMDDRGIGSSKGGNINKMTTPERVEDIKQCIKYLKTRPDIDVSRIGLLG
                     LSEGASIAHMIASQDTSIKVIILLSAPGSKGKEILNHQISNGILDQKNLKKLLKKDIN
                     LRYLYDFDPMVSARLIKQPTLIINGELDKNVPAEGALKLKEEMNKNGNLNVTTHILKQ
                     HDHLLLMVNPEGKINSTRITDETLKIIQDWILTEL"
BASE COUNT         4148 a         2837 c         2727 g         4526 t
ORIGIN      
        1 gggcgtaatt ttttattgtt ttaatcattg tatatctcgt caaaaagaca ttaacaaaaa
       61 tcaatataaa tccacctatc aatacaattc cccatagcat tgaccttgta acttcaaaat
      121 ttagtcctaa tacatcttga atcatacctg ccgctccgtt atcatatatt gctaaaaatc
      181 cagaagatat acaaactaaa gtcattatca cacctgtaaa taatgatacc tgattcagta
      241 actttggctg tctttctggt tttattttat taaaacaaat cctattacca aaatagtatc
      301 cagctaacaa tcccaaaaac gcattgaaaa ctggaagtcc cataaagaat ccataaacaa
      361 aaatattata cataatataa atcgaagcta ttatccaaat aggcaaatta aatcgttgat
      421 taatccagct ttttaagaat ttaatatcta ttaatccacc tattaataat ccacttatca
      481 aatatataaa cagcctactt tcagatttat caagcaaaaa ccatatagtt aaagataaaa
      541 gtcctaaaga caagggaaat atacttccta ttaacaatcc gctaattctt ttttcaaatc
      601 ttttcatttg gcatcctatt aaatattacc cattattatt gcgaatgtaa taataccaat
      661 tattatcggt tgtgtgccaa ataaaagttt aagttactaa aaataaattg ttggaatgcc
      721 ttcgaaatat atttcgtaaa taaagaaaaa aaatttttca ttcgggtaca tatatcgatt
      781 gtttaaccat ttccgattga ccaccatcgc tggtcatcac tacaattccg taagagtatg
      841 caccctttaa aggtgaagta tcaataaatt caagggcatc gggttttaaa cctttcagtt
      901 caacaaaatc tttatcacca tttgctctgt atactacaaa ccaatatttt ttcgtagcgt
      961 tatcgggata ttgccatttc aggattaccg atttgctttg cctgtcatac tgagcggtta
     1021 actgtttaac aggttcacgt ttacccgaat cgtaaggacg ggccgaaacg gcatttgcca
     1081 tttttgatac gtttccagaa ctatcaacat ttgagagtgt gtaatagtaa gttgttttag
     1141 gcataacctt taaatcaaca tacctgttta tgccgggctt aagggtatta attatagtcc
     1201 aagcagaatc gctttcttcc ctccgatata atagctgctc agccacatcg ctacttgggc
     1261 tgcaatgcca ttgtatggtt accgatttat cggtaactga aatatcatgt ataacagccg
     1321 atgccggcgg agtaaaatca ggacgattta actttattac cgatgaaaaa tcagaatact
     1381 gatacctgct atttacagca gcaaccctgt aatagatatt ctttgacaaa gtgttaagcg
     1441 aaattgagtc aataaatacc gtatcgggat gcacttggcc attaacggca ataaattcgt
     1501 gcgaagggtc atttgacttg taaatcctgt atccaataat atttttagag ttaacgggct
     1561 cccacattaa ggttacatga ccatttttat cgattttacc ctttacctct tggggtgctt
     1621 ttggagcagg agtttcagta aggtttgcca aaataggaag cgaaaatccc aaattacctg
     1681 ctgtatcgac agaggctacc atgaagtatg cctcaaaatc gctgcctaaa tcaactacga
     1741 attccctatc atttttggga agcggttcag cagtaatgaa tttgtaaccg taattggcat
     1801 tatcgctttg cgatataaca aaacctttta aatcggcagg ttgcgatttt tgctcccacg
     1861 taagcttaat ttttccgggt gctatttgag ttgatttttt tactataggg gccgcaggtg
     1921 gtgttaggtc gcgggagaac gctttaattt catttggagg gctcagctct ccaaatggag
     1981 taataccaac taaacggtaa ctgtaaacct tgtagtttac tgttgcggta tcaatataga
     2041 gtatgttctc aacagcaacc tgatttgtcg cctgagcata tacaattggc atttgatttt
     2101 tagcaaaaaa tgttttcccg ccatcgtcgg aacgataaag gtaatagcct atatactcag
     2161 agttaagtgc attaggccat gtaagttgga tagaaccatc gccactccta tagctgaaac
     2221 caacagggga ggttaaagcc ttagctggtt gggtttctac cattaagtat gctgtatcaa
     2281 aggccatctc ttttacagtc tcaaccaccg ttacccgata aatgtaacga gaattgggaa
     2341 taacttctct atctacaaat cgtagcccca gcgcgttggc agtaatcaaa tcgttatcgg
     2401 ctgcaaaaag ggcaaatgag tatcggttgg ttaactcatc ggctgcattc ctcaaattga
     2461 ttaggctgtc cgattttagg ggatttggaa caaacgactc accatataag gactgagcgg
     2521 ctatggctga aaacttgttg tttacaccag cgtaattcat ccacgcatcg agctgcatgg
     2581 gtttaatggc ttcgggcgta atagatttgt aaccaaagct ggttatctcc tcaccctctt
     2641 caacctttaa acgttctact cggtaaccaa cagtgttggc aattagccat ccacctggag
     2701 ttgttggggc ccaacgtagg actacagaat ctgatgttgc tcggccaatc aactttatgg
     2761 aagtaaagtt tgccccagaa ttatcaattt gttgtggtag gacctttgct ggtatcaaca
     2821 gaattagaat ggcaatgaac ctaactttat tttgaatgat tttcattgtt tgtgatttta
     2881 aaagttgata ttatactgta agttgccgta tatctcgctg tagttttgcg atactacacc
     2941 ataaccccgg tagctgttgt ttgtaataaa aaaacttaaa ctaccatata ttttcaagct
     3001 ataggaagca ttcaccctga ataccaattg cgtgctttta tcggttacct caacccaatt
     3061 tacccctaaa cttgcagagg tatttaacct atccttgaaa aatcgcttgc caacagtttc
     3121 gcttacatgg gttatggtgg aagtgtataa atccatagca gttttattgt atagcaaagt
     3181 ggtgctaagc gatagcgagc ttggtaaggt tatgatatga ataacattta ccccacttgt
     3241 tttcacatta ttaagattct gtgtgtatac atttttatcg gaagaattct gatagctgaa
     3301 ggttagcata acgttattgt taccctcaaa tgccttgaac gataatcgag gtgtaagcgt
     3361 atatgagtca aacacattag atatgcgtag ggtatcaact ttatgccctg actcaatttg
     3421 gtttttgctg taattaccat caattccgaa aattttggtt acctggtaag tggcattaag
     3481 cattcctgtg aacctactgg tagttgccag gcgattgtcg cgtaaattgt tatagcgtac
     3541 cccaatgcgt gagcggagta tcaacttgtt tccaaacaat tttaggcggg gcgaaatgga
     3601 atactccaac caatcgtttg gcataagggt atacccaagt gtgttaaatc ctggaccaac
     3661 ccagcgcgta tcaagcgaaa ccgaccagta cgctgatggg ttaatatcga tgcttaacaa
     3721 gcctgcgcca tcgatacgtg atgatgcgtt tggagtaaaa agcaactcgg gaatgttaac
     3781 tttaacattg tcgagctttt ctgaattaat gtttgaggag aaaaccgata cggcaccttc
     3841 gcccctgagg gtaacatatt caccgaaacg tatgccaaaa tcaacagatg tcacaaaatt
     3901 ttctgagggc ttaacattga ctgaatcgct ttttatcgat gttgagtcgt cctttgcatg
     3961 gaaaaggttg atattaaagt acgatttaga aagatttcca taccctaaag atacagcgta
     4021 agcgttttgg cgataaactc ctggtgcaaa acttacttta tcaggttcaa cagctttttg
     4081 gctacggcca tatatcgctt tcaatcggaa ttttccgggt gttaactcaa aaccaccacc
     4141 cagtaaacgc atatctccaa aggtgagatc ggaaaagcgg gttgaaaaat agcctgcatg
     4201 caaagtgagc caattcgaaa ttttaggact aaccccaaat tggttaaaag gttgctggaa
     4261 tcgggtttgc cctgtagtaa agtaaagttc aaagggtagt tccaccacat cccaaagcat
     4321 tacgcttgtc ctaataattc catactgcat atttgttggc tggcgcggat caataccgtt
     4381 cgatgaatag aagttgctgg ctagggttgc tgaggttttt acatcaattt tcgacttttt
     4441 tacctcctct tcctgagcat gtgataccat gatgaaacaa ataaccatcc atgctgttat
     4501 actaatttta agaaatcttt tcatactctt cagggatttg ctttcagtag atgaatggct
     4561 tactgatggt aacaggctcg gtatcgataa ccctacatcc aaaataatca tagtagaaac
     4621 tcaacgggta ggttcccctg tacattctct ggtagttgct attaattata gcatttagcc
     4681 tatttctttt agccgcatta attgttataa gggggttcga caaggttgcg attgcgcggt
     4741 atttaaggtt cataaagtcg gtagggacaa taataccatg gttataggtt aatttgaagt
     4801 atggagccga agacgattgg aacaaactta ggttttgcgg ttgatttgat gttgaaaaac
     4861 caccctgcac aagagcaacc gccccagttg atgattgtcc agttcctcca gttcctccta
     4921 ttccaaatgg ttgaccaatt gcatttactg aaaccgacat actagtattg aaatcgatat
     4981 caacaaaact taatgaagga tcgctcatat actggttata gttaattggg ccattccagt
     5041 atccgttgct ttgcatccat gcaatttcat cgtaaaccaa tgggttagca aactgattat
     5101 gccactggga gttgcgttgc caggcattaa tcttaacaag cggcccaaat ctattaacag
     5161 tgcctgacga tacccaggta attggcctaa aatcaaactc atcgaaacac tcttccccct
     5221 ggtaatttgc tgtaaggcgc tcaaaattcc catttgtctc aaccgaggtg gatgtatgat
     5281 taaatgttgc caatttttgg cttaaagtgt tgaacctgct ggttgagaac caaactgcat
     5341 aaagcagcct ctcgcccgaa ttaagatttg aattttgatt tatagttctc ctaacaagat
     5401 ttacttgaga accgcctcgg ttataaatat tcgactcgtt agtagttaaa gaatgttgca
     5461 tacctccact atttaaaaac tgttgaccaa gatcaagcat gcgttgtaaa tttggatcat
     5521 ttggaatctc ctttctgata aactgaatat agtatgattt actgttgatc agattaggaa
     5581 tatcaaagag caaagtgttt gttgaagagt tataggtaag gggcacatct actggcatta
     5641 acgcaatatc ggtgggcatg aaacgggcaa tgaactgcac attgtaaccc tcacggggtt
     5701 ggaacaagta gctttgcccc gatttcagct gaaccctgcc ggcacggcat tcatcctgta
     5761 ggaaatactt atgcccaatg accgggtagc tataggcaac attttgctcg gtaattcggt
     5821 cgggtcgtga gccagtttta aaggtagcag ttacctgctg ttttattacc tgcccatttt
     5881 ggtttgtagc tggacgccat accccattaa caagttcttc gccataaact gatgccaata
     5941 ccttaaagtt ggtatgccct tccagcatct cgaatggttt atagtatgca gaaaatcctt
     6001 ctggatcaat acttaatctt ccaggaatag agtcgttatt agttgcatta actattttaa
     6061 attggtccat cttaatcctg aatgttcgaa tgcgggtttg accattgttt gtcatttcct
     6121 taagttggaa aggttcattt agcttgaagt tcaaagccac ctgtgcctca attgaaacat
     6181 ctactccatt ttgcccgctt acaggactta tatcggaaat caagtcgatg cgggctaagg
     6241 gattatcgcc aagcatttgg cattcatcgc ccatactaaa atggtaattg cagtaaccct
     6301 taacggcacc acctagaatg cggtaatttc cgccaatagc ccctttaatc caggtcgggt
     6361 taggtaatcc tgcctcaagc atggctgctg tgttcaagct gagaatttca aaattaccac
     6421 tggtaaacca aacatccacg tgcataccta ttgaagcatt aaggtaggca taaacctgac
     6481 cggttgcgta ccagccattg atacccagct caccacttcg accttcgcac gtaaccccgt
     6541 tatacttaag cattgccata tcgtaaccag cctgcgcagc cacatcgcca taaaaaatca
     6601 tgtactgttg acgccctgtg ttaaatccag tgcttgcacc caaagcaatg ccattacctt
     6661 gttcaatcat gaaactacgg ctttgggtat aacccgggaa tgcttgctga atttcggctg
     6721 gtaatgctgg tgggggcggc agctgctgac caaccattaa gtaagcatcg ctcctaagcc
     6781 agtcggcaaa ggttaattgc atacgtttag agggttctcc aaacttaatg taccacaaat
     6841 caggtgcaaa atgcataacc atttgaccag aacctgtaag gggttgataa tctactcttg
     6901 cgttaaaaac accatgaagg gtttgctcag aaacagtgta gtttatatcg gcatcggcaa
     6961 gtattttggc tttgttccgc tcgttgatac tgcaaagcat gtatccattc cctagcaagg
     7021 aaaccgagct caagccacca ccattttgga actgtgcctg taaagtaaaa tcaccgttaa
     7081 aggtttcggc cgagggggat gttcccataa taacctgtgc cgataatcct aacgaagccg
     7141 aattgtcggg aacataggta aagcctgagt tggtttttga cggattttta ctgctatcag
     7201 gagcaaaagc ctgttctgct aggttagtag taccggaccg gtgcatctta taccatgccc
     7261 caccaccaaa cccatacatt gaaaaattag gaaatacagg taagccagtt ggtaaaatgg
     7321 ttttggcatc tatataccag tacctaaatt tgttaaccat tccaaactgg gcagttgccg
     7381 aggttgtaat gttatcgata aaacttgctg ttactacccc tcgataacca tcgccatagg
     7441 tggcatcgga ctggaaaagg tttaaccctc cacgcacctt aacagcacct aaatcagcat
     7501 taaccccaat tgaatcaagt tcaataccat caaacataaa acgttgccgc cctcggtcat
     7561 taatcagctt actccagaag gttagcgttg tggtgccatt caccgcattt gaccctggct
     7621 gtaaaccgat attcaggtta aagcgaaccc ctggaccaaa agcaccgttg ctaaaacggg
     7681 ttacagaact tacgccatct atgttaaccg gaaatccggc catggagtgt tgagggctgg
     7741 caaaatccca gttaccattc tcaacgtatg gttcgtatgt tctaaacgta agcccttcaa
     7801 cctttatccc tttaaaatct aattttgata aaatatcgtt agatccttca aacgttaaag
     7861 tagcattgag attggcttcg gctttaacat cctttttatt gtaagttact ttgattgagg
     7921 ttgcgggatc aattttcatt ttaagcggaa gccatgataa tgggatttcg cgctcataac
     7981 catgctcggt gttaacaact agctcaagat tgtaatcatt actaatggtg ttattgtcct
     8041 ggttaatgct ccggttgtat aatccattat aaacaaatag tgtatcggta aacgatgttt
     8101 taattctacc cctcattcgc caattgctta aagtattatt aatgatctcg gccttgaggg
     8161 tatcaatact tcctccccag cctccaaaat caccatcaaa ttcctggaag aggttttctg
     8221 catagagttt tccggttata ccattaccgt caatgattag gtttgaaacc tgtattttag
     8281 ggtttgcatc gtcaaatgtt tttaaattat cgggaagtgt tatctcggcg ttcttaatat
     8341 aaaaaccttt ccaagtattt gttttatcgc ccgtgtaacc gtttgggaat gtaatgttct
     8401 caggattttg gatggttgta taatcgaaca ccatgctatt taccttcatc ttaaaccctg
     8461 gtagatgtgt aaactcacag tcatccatat ttgcgtttag tatccatccc catgaatttc
     8521 cgttcttttc agcccttccg gcaaaatgag ccttaacgtt ttgcaacgag tcatcagggt
     8581 tggccatttt taaccaggtt ttggggaact caacctctgc cttcacctca atgtaatgta
     8641 aacctttaca gtcccacttg gcataggtac ccgaatcggt tggtgttggt gatttaaaaa
     8701 ggaaactcca actttcgtcc gacctataac caaagtcatg gacaaggtat aactcggcac
     8761 gtttcatacc gccaagacca tccttgtgga aacaaaggtt tttagctcca aaaccgaaac
     8821 ctacatcagg ccccaacaat gggagttcag cccaagttgc tgcatttaat accgcttgtg
     8881 ttggggtaaa tttcatggca ataattgcta ctacggtatt atttccctca atatttctat
     8941 ccaggcctat gggaagggta actggatttg tattaattaa gctgcttacc aaacgtccta
     9001 aactagacga ccattcataa attgatttca gttgattatc ggccatgtta agaacctcgc
     9061 cttcaaaatc atttccatcg gcttttgagt atggggcagc gggatcgatt ttggcatata
     9121 cctctccctg ataaacttta ttatcgccgt taactccaat atttgaaaat tcaactaaga
     9181 taggcgctcg taaataggga acttcaattg aaccttcacc cgaaagtgat tcgggagttc
     9241 cgcttacact tatcagtttc atggtgaaat ttccaatggt aatggtgcta tccttagcaa
     9301 gcgtttgggt ccctggagta gtatttgtag gtgcttcggc aaagcaattg ccagggcaag
     9361 tattttcatc ctttgccgat gttttaattt gaaatacacc ctggttcgat attaccgaat
     9421 cggctttaca cttacaacca tcagctctaa ttgagtcttt attatagttt aaccggaaat
     9481 tccaagtgta gtaggtgtta tcatctcctt tgaattcttt tgaattagaa tcgccattta
     9541 taaatggcaa gtcccaacgg gtatacccgt ttatatcggt tgaaagttca gctattttat
     9601 tgttttcatt accagtaaaa ctttcatcaa acactggttt ttcctttata acatcagaga
     9661 gcttctgcga ttcggatgtc attttccaaa tcctaagtct acctccggaa atatgtttct
     9721 tttttatagt tggtttaatt agtatagcaa atgttggtgt ttgattttca gtccaattac
     9781 cattgtttgt tggtttgtca acattcatgg taaaacatga tgttaaacta tcggcaacta
     9841 ttctaaaact tgctggttgt gaagtaaagt aagcatcgtc aattgggtca aggaatttta
     9901 cacgccagta atactttccg gtatccgata actggctaat cctatggttt attctttggt
     9961 ataattctgg acaatcgctg cctgttgaat agtgccctgt tgatggaagt gtaatggttg
    10021 attggtaaac caacgaatcg aacgattgct tttttgatac ttcaaactta atttttgctg
    10081 tagccatagt agtagcgtaa gcaccattga aaaatggcac gttgaatagg ctgtaatttg
    10141 gatagttaag ctgttggggt tgatttacct caaaggatag ctcaacacca ttgccgattt
    10201 ccatcaaggt gtcagctgca ggcttaaaac tcttcgcctt acttaagcct actgcaaaag
    10261 atgtttctct tgaattaacg gttgtggttt taccgtcggc ggttgtaatt gttgcatcaa
    10321 ccttccagta atattttttc ccttgcttta atgctgccat gaatggggct atagcaccgc
    10381 ttgaatagtt acatacaacc actcctgcca gttcaggagt aataggactt tcaaactgac
    10441 gtggtgaata ggacataacc ttagggtatg tgtatattgt agcagagcca tcttccctga
    10501 ctgaaagctg aagagccacc tgtgttaccg tggagttaaa tggagaaatt ttagcaatta
    10561 gctgtgggtg tgcccatggc aaagtatcgc cggcaacggg gtaaatgtta tcaatggaaa
    10621 attcctgctc atctttacct accctaaaag tggcaacctc actcctgccc tggttccctc
    10681 caattggctt gccagatgga tcaaaagctt gcactatcca ggcgtactgg gttacctgtg
    10741 ggtaaaaatc aaaaggtaag tcacctggta ttgactggta aaaggttgag gtaatatcgc
    10801 ttttctcaaa taaaatggga tttacttcga ttgctgttcg gggtgtttgc ccttgaaaga
    10861 caggacatac tttaatctta taacgcggct gaactgttcg aggattatag tttgttaatg
    10921 gcatccaacg gaaaatcggt atagatgaca gtattatttc attatcggag ggtgaaataa
    10981 tggtgggagg ttcgggtatt tgggtaataa tattcataca gtattcacct cctatagtga
    11041 tattattgcc gcggtaatca taaacactaa cacacagttg atagtttcct tcgggtaaag
    11101 catttgtgtt aagggcaatt cgctcaatgc tcctatcata ctctactgca ttcatattga
    11161 gtacctgtgg cccattaagt attaaaaccg atggcggttt tagtatagta aaccttggca
    11221 aatacctgct acctgttttg gtgcttgcta tcactttgcc tttttcattg gtaatggtaa
    11281 aaccagctgt acagttagga tactcatcgt tacccatgtt ggttataact atctggaaaa
    11341 tggaaggatc atttacccat tcactaatct ccgatggaag tggattgcgg agaatcatct
    11401 ttaaatcaac ctgggctaat gaatttagac tgaaaaatac gagaattaaa gatattgtta
    11461 acttaatttt atacttcttt ttacttatat gaaattttga catgtaattt ggattttgta
    11521 tgaataattt tagaaaaata agcattaggg tacaattatt ctttagcatc tgcatgaata
    11581 tcttttaagt ttttattaaa caaaactaat aaaaaaatga tttattgagt cacctccaaa
    11641 aagtaatttt aggaaaagta gcaaaaattg ttgaaaaaat ggtagataga aaaaaattga
    11701 catctcgaat gaaaattttc atgtaatttt taataaaatg ggctgttttt gtatttttcc
    11761 cttcttttgt tcaactattt ttcctcttgt tattatatat cttgctatcc ttttcatatt
    11821 tatccatatg catctcatta ttgcccacat tttgttcttg tacattcccc tgtatcgtgt
    11881 tttactgttg ttggtgtaat aggcaaactg aaatatggtt gccttcacat tattacgcat
    11941 attttgtttc tcaaccggca tgtttgctat ttgtttgcgt agcttcgacg tctctaacgg
    12001 tcggcggtat ggttagttgc cgatttcgaa gcacttactt atcaagttac aactactttt
    12061 gacacgagct ataccgctcg aatatccact gcaccggcaa ttaattatac cgtttattag
    12121 cgtttcgttg tttttttctt ttctaatttt ataaatccat aatatattaa tccgctacat
    12181 attaatagtc caagtattgc aggaattatt atagacttcc ctgattctcc agcattttga
    12241 atactccaaa ctatcttacc agtcgccatt aaaactacta caccaagtcc cattaataaa
    12301 ctacgtttcc agaaagcaag tcctaatgca aaaaatgaaa ctggaaccgt aataattaga
    12361 attatttttt tataatccca ttgactataa agccagtctt tctcgaattg gagaaatgtc
    12421 tttacattgt cacttacgaa ttctggttta ttaaaccaaa acatacttgt aaaaagacag
    12481 aatacagtta tagaaatccc tgtccagttg cgtttatatg caaaaaaaca aaatggtatt
    12541 aaaaaaattg gtcttatata ccaactcaat tggttgtgat gtctgtcgaa aacccaatca
    12601 aagaaagttt gatttgtcat taatacaaac aagaataaaa ttgtcaatgc caaaaatagt
    12661 aatgcgataa ttttgtcctg attaattaat ttcatattct ttcatttaat aattgtctgt
    12721 ctgtttctaa ttctttacaa tgtgcgccct gacggcccag cggtatgttt tagtaagcgt
    12781 aacccgacga cagcaccggg caggatcctg gtacctcgtc ccgagtactc gggagggcca
    12841 taaagctacc acgccgtgcc ccgtatgagc gaagcgaaat cgggggtgca aggagggtat
    12901 ggcgcgcgtt tttgccgtca tggtacaaat tttattgagt tctggcgcgg tcgcggtaca
    12961 aattagatcg gagccgcgaa cttatcaccg gttacaaagc tggttgcggg cactggtccg
    13021 ccaactggcg gatcgcggta caagctgtct cagaaccgaa aatctgtcac gatgcaggtt
    13081 ttaaattgtg ggcagatgcg ggtatccggg tacaagattc agcaggattt gtctgagaag
    13141 cagggttttg caaaaaccat ttttattttc atgctgtcat agttctgtta ggatccaatc
    13201 ttgaataatt ttcaaagttt catctgtgat tctagtcgaa ttaattttcc cctcgggatt
    13261 caccattaat aacaaatggt catgttgttt taaaatatgt gtggttacat taagattacc
    13321 gtttttattc atttcctctt ttaatttaag tgcgccttca gcaggaacat ttttatctaa
    13381 ttcgccattg attataagag ttggctgttt gataagtctg gctgatacca ttggatcaaa
    13441 gtcatagagg tatcttaggt taatgtcctt ttttaaaagc tttttaagat ttttttgatc
    13501 tagaatgcca ttactaattt gatggtttaa tatctcttta ccttttgatc caggtgcaga
    13561 tagaagaatt ataaccttta tagaagtatc ctgtgaagca atcatatgtg caattgatgc
    13621 tccctcactt aatcccagta gcccaattct agagacatcg atatctggtc tggtttttaa
    13681 atatttaatg cattgtttta tatcttcaac tctttcagga gtagtcattt tattgatatt
    13741 accaccttta gaggatccga taccccgatc atccattctc aatactgcaa ttccttttgt
    13801 agaaagcaaa tcagcaattt gtcggaaagg tctatatgcg gttattggat tatcaggttt
    13861 tgaattgtct ctgtcatgag gtgaactacc agttattaat agaacacagg gaaattcttt
    13921 tatttcgtct tttggaatag tcatggttcc aaccagaatg tctccacaat ccaatgttat
    13981 ttcaacagtc tccccaatat aatttgctcc ttcaggaggg tcataactta ttggcttatt
    14041 atttgagcaa ctgaattgaa aaaccaggca aacaaaaatt gtaaataaga tgtctatttt
    14101 aaatctgttc ataaactgta taggtggaga gaatcttttt tatttgtagt gagaatattg
    14161 cggatagagt acatgttacc gataactgta aatattaatt gaaaagtata ccacattctc
    14221 gttgaaaagt ataccact
//
LOCUS       DAOOWJ010000069        14074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4350_length_14074_cov_9.551609, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000069 DAOOWJ010000000
VERSION     DAOOWJ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14074
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4350_length_14074_cov_9.551609"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLW22_07760"
     CDS             complement(<1..584)
                     /locus_tag="PLW22_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOU74639.1"
                     /translation="MSEQIKHECGIALIRLLKPLEYYQLKYGTWRYGLHKLYLLMEKQ
                     HNRGQDGAGLTTIKFDLAPGKKYIDRERSNSSTPIKDIFDAVNKGINKYSSKPELLND
                     PVFAKENIPFAGELFLGHLRYGTFGNNSIDYVHPVMRDNNWKSRTLVLAGNFNMTNVD
                     ELFKKLVDLGQHPRDYSDTVTVLEKIGHFLDEEN"
     gene            complement(636..1598)
                     /locus_tag="PLW22_07765"
     CDS             complement(636..1598)
                     /locus_tag="PLW22_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU74640.1"
                     /translation="MGEYINKGDKATRLGFGEGVAEAGGEYPNLVVLGADITSSVGLN
                     LFAERFPERFFSFGIAEQNIAAVAAGLALTNKIPVFSTYGVFAAFRAADQIRISICYN
                     NVKVIIGGAHAGISVGPDGATHQALEDIAVIRSLPNITLISPADANQAKAATLAAIRQ
                     VEGPVYIRFGREPVPNFTEPNQVFEVGKGQVLKAGTDVAIVATGHMVWEALEASKHLA
                     KQGVNAMVVNIHTIKPIDKQLLVDVAKATKRVVTVEEHQVHGGLGSAVAEVLSQEYPV
                     PMKIIGMPDCFGESGTPAELLQKYGLNANSIAQHVFQFVNRFKQ"
     gene            complement(1591..2439)
                     /locus_tag="PLW22_07770"
     CDS             complement(1591..2439)
                     /locus_tag="PLW22_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU74641.1"
                     /translation="MATPAIRQLQKTSLKIRQDVIRSLTAAGSGHLGGSLGLADVFTV
                     LYFNTLNHFPKEPNHPNRDRLILSIGHVAPVLYATLANAGYFPHEELLTLRKLGSRLQ
                     GHPGRDHGLPGIELSAGSLGQGLSVAVGMALSAKIDNSKWQVYSVHGDGELQEGSVWE
                     AAMSAAHYKLDNLTAIVDRNFCQIDGYTHKVMELEPLKLKWEAFGWHVFECNGNSIDE
                     LIDVFGKTRNITGKPKVIIAKTQMGFGVNSIQGDYRWHGKAPTTQEAEKFLEELEKYY
                     HELIHG"
     gene            complement(2441..2902)
                     /locus_tag="PLW22_07775"
     CDS             complement(2441..2902)
                     /locus_tag="PLW22_07775"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU74642.1"
                     /translation="MRKIFLLLVLLGLSFGIFAQNTNGTTPIPIVNELQAHKAGEGTV
                     TIRQSESITQIHNIRYLHNAKSPGMEGYRIRIFFELGQFARKNSEDIMQAFMEKYPGV
                     PVYQNYQNPYWKVSVGDYRSREEAIKFYNQIIKDYPKAFIIPEHINFPPLN"
     gene            complement(2942..3460)
                     /locus_tag="PLW22_07780"
     CDS             complement(2942..3460)
                     /locus_tag="PLW22_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOU74643.1"
                     /translation="MKNFSFILLLSILLFALAGCGKYNDLNIHGVKEYRFRGMKDGVI
                     FLNLTLDVENPNRKSIVIKDIHLKAWLNNRELGKLRSSHKLKIEGKSRKDYEVPLEIV
                     LRTPADAFKLIGEGKNLISVIEVEGYIKGGKFPVVKKINIPRQPLSELANSFQKKFVI
                     TDTLSVQPAVMP"
     gene            complement(3598..4344)
                     /locus_tag="PLW22_07785"
     CDS             complement(3598..4344)
                     /locus_tag="PLW22_07785"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU74644.1"
                     /translation="MSGHNKWSTIKRKKGALDAKRSKEFSRIIKEISVAVKEGGADPE
                     GNPRLRLAINNAKGVNMPKENILRAITKAEKDPENLQELTFEGYGPGGIAIFIECLTD
                     NNNRTISAVRSTLTKKGGNLGTNGSLAFLFSRKGIFTIAKDKINLDEVELDLIDAGAQ
                     NIDDNGDFYVVTTEMEDFGRMNKKLEELKIEVENASLQRIPNDFKSVDKDTAMKFLKL
                     VEELEDNDDVQQVYHNLELTDEVMAAMDNE"
     gene            4495..5892
                     /gene="miaB"
                     /locus_tag="PLW22_07790"
     CDS             4495..5892
                     /gene="miaB"
                     /locus_tag="PLW22_07790"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOU74645.1"
                     /translation="MDFNAEDANIFESALSDDSSRPKVYIETYGCQMNVNDSEVVASI
                     LIANGYAITSNINEADVILINTCSIRENAETRVFGRIDLFGQVKKSKPSVLVGVLGCM
                     AERLKDQLLEEKKVVDLVVGPDAYRELPVLLRAAEDGQKGINVLLSREETYADISPVR
                     LDKNGVSAFVSIMRGCNNMCSYCVVPYTRGAERSRDPHTIVREVQELVDAGYREVTLL
                     GQNVNSYHWEEQKGLIKHKVNFSDLLEMVALVDPRLRVRFSTSHPKDLTNEVLYTMAM
                     YENICNHIHLPVQSGSTRILQLMNRKYTREDYLDRIKAIRSIIPDCSISTDIIAGFCT
                     ETEVDHDMTLSIMREVGFDFAYMFKYSERPNTKAARQLKDDVPEEVKTRRLMEIIELQ
                     GKLSEQSKKSDLGKRFEVLIEGESKKSKDEFFGRTSQNKVVVFPKENHKIGEFVFVEI
                     TDCTPATLIGRIVQE"
     gene            complement(5894..7090)
                     /locus_tag="PLW22_07795"
     CDS             complement(5894..7090)
                     /locus_tag="PLW22_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU74646.1"
                     /translation="MGSVFETAGRALGEVVYQNRNLDQVISYWIAQNKLWEPFDKYQF
                     AVIVEETTRWWRLLNELHDSEFPKFGSHTHNIVAIYTTLTPFGFPEERKIPKHFKKKL
                     QNKLNQLSNNRAIKESIPDWLDELGFKELGEKWPKEIHSLNHKAPIVLRTNLLKTNVQ
                     ELNNFLKREGYKTKIIPNTHAILLTESANIFKSEAFKLGMFEQQDFSSQQVVPFLNVE
                     PGMRVIDACAGNGGKTLHLASLMNNQGRIIALDTAPWKLEELKRRSRKAGVTNVETKA
                     IETTKTIKRLQDSADRLLIDAPCSGLGVLKRNPDSKWRLTPENIAELRKTQRQILEKY
                     WSMLKVGGCMVYATCSILPSENEEQVKWFLEQMNGRFVLEEEKNLSPSQTGFDGFYMA
                     RLKRIK"
     gene            complement(7146..7874)
                     /locus_tag="PLW22_07800"
     CDS             complement(7146..7874)
                     /locus_tag="PLW22_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74647.1"
                     /translation="MKKITLSKEEIEDLKDLYQSEIDRAQRRIDSLKSILRKIEKGEL
                     GDEKEVAAPKKRGRKPKVKEATEPTAPKKRGRPKKITAEADKSAQTKEPKKRGRKPKV
                     ASETKTEKPKKGRKKIAKKKVKTSPPKKRGRKPKSLRKGLKPTVGGEKIKWNNLIIDI
                     FKSNDKLLQAKDLTVGALEKLQLPEVEKDRTRMAVATNLTKLTKYEKKILKYTRPDDK
                     IAYYGLAEWFNEDGTLKPEYQNKF"
     gene            complement(8778..9779)
                     /gene="fbp"
                     /locus_tag="PLW22_07805"
     CDS             complement(8778..9779)
                     /gene="fbp"
                     /locus_tag="PLW22_07805"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOU74648.1"
                     /translation="MNGYNVKTLTQFIIERQADFPYATGEFTRLLYQVGVAAKLVNKK
                     VNKAGLVDVLGEAGNINVQGEEQKKLDLLANWQFVQALKNSRECCGVASEEDQEIITW
                     DDELSKDGNYIFCMDPLDGSSNIDVNVSIGTVFSIYRRISPRGTKPTMEDFLQPGNKQ
                     VAAGYVIYGSSTMLVYTTGRGVYGFTLDPSIGEFCLSHLNIVTPPDGAIYSINEGNYN
                     HFPEGVKKYIEYCKQEDKPTKRPYSARYIGSLVSDFHRNLLKGGIFMYPPTASAPKGK
                     LRLIYECNPIAFLAEQAGGIATDGKNRILDIQPESLHQRVPLYVGSKNMVQKLQEFL"
     gene            9970..11247
                     /locus_tag="PLW22_07810"
     CDS             9970..11247
                     /locus_tag="PLW22_07810"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU74649.1"
                     /translation="MKPIRVFKFGGASVKSAGAIRNLANIVKRFSTENLVIVVSAMAK
                     TTNALEEVLMYWMNADLTAMNTKLKEIKQFHLNEALDLVEGDSNHLLIAELQALFNQL
                     ETQFGQTPTKRYDFHYDQVVSFGELFSTRIVSHYLNFIGIANTLVDARRCLRSDTSYR
                     EGVIDETTSRLLCQREFDFSSANIFLTQGFIAGTEVGTTTTLGREGSDYTAAILANLL
                     DAQDVTIWKDVAGVLSADPKIFSNAVKLSFVSYQEAIELAYCGAQIIHPKTIKPIQNK
                     KIPLYVKSFMEPDAQGTIIAHSDTPVKLPPVLVLKRNQVLITLSPRDFSFVIEDCLSK
                     IFALLYKHRIKVNMVHNSAINFTVCVDNDKLRLGDALEEMKHEFAVRYNSNLELLTIR
                     HYTPEIIENFIGNRVVYLQQRTRSTARFVMDAS"
     gene            complement(11251..11739)
                     /locus_tag="PLW22_07815"
     CDS             complement(11251..11739)
                     /locus_tag="PLW22_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74650.1"
                     /translation="MNIKFKLILLAIVFESCSNPQPKNVIVDITGKEQDLTLIADSIK
                     YEVVVQASENDIWESERLQGYRNHKSFIDGTFKGILSGKLKVFDYTTNEPLTVEEVRK
                     IIEDHKIDASQIGKLLFTEQWFTDKQGHLHKKILSITFGKSEYSKQGTFKGYSALFTV
                     KY"
     gene            complement(11744..13090)
                     /locus_tag="PLW22_07820"
     CDS             complement(11744..13090)
                     /locus_tag="PLW22_07820"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOU74651.1"
                     /translation="MGRLAIINATIINEGKTFKGNLIVNGETIEHISTAPSSVDKFLP
                     EEIINAEGLWLLPGVIDDQVHFREPGLTHKGDIYTESKAAVAGGVTSFMEMPNTKPQT
                     TTVELLHDKFKIASQKSLANYSFYLGATNDNIDEITKVDPRNVCGLKVFMGSSTGNML
                     VDNEDALEKIFRFSPTIITTHCEDEDTIRKNLEKYQGLYGNNIDIKYHASIRSAEACY
                     LSSAKAVRLAKRHGARLHILHLSTANELELFNNSIPLAEKKITAEVCVHHLWFNNNDY
                     DRLGWRIKWNPSVKTETDRLALIQGLKNNLLDVVATDHAPHLISEKDNVYTSSASGGP
                     MVQHSLLAMLELSRKGYFTPELVVEKMCHSPATLFRIEKRGFIREGYFADLVLVNPNK
                     SQAVAKENLYYKCGWSPLEGETLSHTVEMTILNGRIVYSKGVFNETIKGKALTFNP"
     gene            complement(13101..13850)
                     /locus_tag="PLW22_07825"
     CDS             complement(13101..13850)
                     /locus_tag="PLW22_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOU74652.1"
                     /translation="MSFKEKLVIIPTYNESENISRMIGKVMSLEGNFHLLIVDDGSPD
                     GTASIVKEKQKEYPDRLFMIERSGKLGLGTAYITGFKWALANGYKYIFEMDADFSHNP
                     DDLINLYNACVNGADLAIGSRYVNGVNVVNWPIGRVLMSYFASKYVRMVTGMKVMDTT
                     AGFKCYKAEVLKAINFDKIKHIGYGFQIEMKFTVWKLGFNIVEVPIIFTDRKFGASKM
                     SGGIFNEALWGVVNMKLKSLFVSYKKLHASN"
BASE COUNT         3856 a         2968 c         2870 g         4380 t
ORIGIN      
        1 tggttttcct cgtcgaggaa atgtccaatt ttttcaagta cggtaaccgt atcggagtag
       61 tcgcggggat gctggcctaa atcgacgagc tttttgaaaa gctcatccac atttgtcatg
      121 ttgaagttac cagctaggac aagcgtacgc gatttccagt tgttgtcgcg cattaccgga
      181 tgaacataat caatggagtt attaccaaag gttccgtagc gcaggtgtcc caggaatagt
      241 tccccggcaa aaggaatatt ttccttggca aaaaccggat cgtttagcag ttcgggtttc
      301 gatgagtact tattgatccc tttgttaaca gcatcaaaaa tatccttgat gggagttgaa
      361 gagtttgagc gttccctatc gatatacttt ttgcccgggg caaggtcgaa cttaatggtg
      421 gttaaccctg caccgtcctg cccacggtta tgttgctttt ccataagcag gtaaagcttg
      481 tgaagtccgt atcgccaggt accatactta agctggtagt actccaatgg tttaagcaac
      541 ctaatcagtg caatcccgca ttcgtgttta atctgctcgc tcatatcttc ctaaattatt
      601 caattaaaaa aggtagatag aatctacctg aagttttatt gtttaaatcg atttacaaac
      661 tgaaaaacat gctgggcaat tgaattggca ttaagaccgt acttctgcaa caactcagca
      721 ggtgttcctg attcgccaaa acaatcgggc atgccaatta tcttcatggg gacagggtat
      781 tcctgcgaaa gcacttcagc cactgcgctg ccaagtcctc catgaacctg atgctcctca
      841 actgttacaa cacgctttgt tgctttagca acatcaacca atagctgctt atcaataggt
      901 ttgatggtat gaatgtttac caccatggca ttaacaccct gcttggcaag atgctttgat
      961 gcctcgagtg cctcccacac catgtgaccg gtggctacta ttgcaacatc ggttcctgct
     1021 tttaggactt gtcccttacc aacctcaaac acctgattgg gctcggtaaa attgggcact
     1081 ggttcgcgtc cgaaacggat gtaaaccggc ccttcaacct gcctgatggc ggcaagggtg
     1141 gcagcctttg cctggttggc atcggctggt gaaattagtg tgatatttgg gagcgaacgg
     1201 ataactgcaa tatcctcaag ggcttggtgt gttgcaccat cggggccaac cgatattccg
     1261 gcatgggcgc caccaattat tactttaacg ttattgtagc aaattgaaat tcttatttga
     1321 tcggcagcac ggaatgctgc aaaaacgccg tatgtagaaa agacaggaat cttattggtt
     1381 aatgctaacc cagccgcaac agcggctata ttttgttcag cgataccaaa ggagaagaac
     1441 ctctcgggga aacgttcggc aaaaagattc aatccaaccg aggaggtgat atcggcccca
     1501 agcacaacca ggttggggta ttccccaccg gcttcggcaa ctccttcacc aaatcccagg
     1561 cgggtagctt tatcaccttt atttatatac tcacccatgg attagttcgt ggtaatattt
     1621 ttctaattct tccaagaatt tctctgcctc ctgcgtggtt ggggctttac catgccaacg
     1681 gtaatcgcct tgaatggaat taacaccaaa gcccatttgg gttttagcaa taatcacttt
     1741 aggtttgcca gtgatgttgc gggttttacc aaaaacatca attagctcat caatactatt
     1801 gccattgcat tcaaatacgt gccacccaaa tgcctcccat ttaagtttaa ggggctcaag
     1861 ttccattact ttatgggtgt agccatcaat ctgacagaaa tttcgatcaa caatggcagt
     1921 caggttatcg agtttgtagt gtgcggcgct catggcagcc tcccaaacgg atccttcctg
     1981 tagttcgcca tcgccatgca cagagtatac ttgccatttg gaattatcaa ttttagcgct
     2041 gagcgccata ccaacagcaa ccgataaccc ctgacctaac gaaccggctg agagttcaat
     2101 tccaggtaaa ccgtgatcgc gtcccggatg gccttgtagc cgtgaaccta gcttgcgtaa
     2161 ggtaagtagt tcctcatgcg ggaagtatcc tgcattggca agcgttgcat acaaaactgg
     2221 tgcaacatga ccaatggaaa ggattagcct atcgcggtta gggtggtttg gttctttagg
     2281 gaaatggttt agggtgttaa agtatagaac ggtgaaaaca tcggcaagtc caagtgagcc
     2341 tcccaggtgt cccgatccgg cggccgtcag gcttcgaatt acatcctggc ggatctttag
     2401 cgaagtcttt tgcagttgcc ggattgctgg tgttgccata ctaatttaga ggtggaaaat
     2461 ttatatgttc gggaataatg aaagctttgg gataatcctt gataatctgg ttgtaaaact
     2521 tgatagcctc ttcgcgcgag cggtaatcgc ccactgatac cttccagtat gggttttggt
     2581 aattctggta cacaggcact ccaggatatt tttccataaa ggcctgcatt atatcttcag
     2641 aatttttccg ggcaaactgt ccgagctcaa agaatattcg aatcctgtac ccttccatcc
     2701 ccggcgattt ggcattatgc aagtagcgaa tgttgtggat ttgagtaatt gattcgctct
     2761 gcctgatagt tactgtacct tctccagcct tgtgtgcttg aagctcatta actattggta
     2821 tgggcgtggt accgtttgtg ttttgggcaa aaatgccaaa acttaaaccg agtaacacta
     2881 ggagtaggaa aattttcctc ataaagagta taatttcgtt gcaaaaatag caaaaattta
     2941 tctatggcat tacagcaggt tgaaccgaaa gagtatcggt aataacaaat ttcttttgga
     3001 atgagttggc tagctcgctt agtggctgcc gggggatgtt aatttttttt acaaccggga
     3061 attttccccc tttaatgtag ccttcaacct ctattacgct aatcaggttt ttcccttcgc
     3121 ctataagttt aaatgcatcg gctggggtac ggagcacaat ctctaatggg acttcataat
     3181 cttttcgact tttgccctct attttcaatt tatggctact ccttaacttt ccaagttccc
     3241 tattgtttag ccaagctttt aggtgaatat cttttattac aattgatttc cggttagggt
     3301 tttcaacatc gagcgttagg tttaggaata ttacaccatc cttcatcccc cgaaatctat
     3361 actccttaac accatgaata tttaaatcgt tgtatttacc acagcctgct aaagcaaata
     3421 gtaaaatgga aagaagtaaa ataaaagaaa aattcttcat gctttatgtt ttaattagta
     3481 agagcaaatg ttataccata cccacaaaag taatatgatt gattggccac ggtatacagt
     3541 gtatactaaa aataaaaaaa actcccctgc attggggagt aaaaggtttt attgtgttta
     3601 ctcgttatcc atggcagcca taacctcatc ggtaagctcc aggttatggt aaacctgctg
     3661 aacatcgtca ttatcctcta gttcctcaac caacttcagg aacttcattg cagtatcctt
     3721 atcaaccgat ttgaaatcgt taggaatacg ttgcaggctg gcattttcca cctctatttt
     3781 cagttcctca agctttttat tcattctgcc aaaatcttcc atttcggtag taaccacata
     3841 gaaatcacca ttgtcatcga tgttttgggc cccggcatca ataagatcga gttccacctc
     3901 atcgaggttg attttatcct ttgcaatggt aaagatgcct tttcggctaa aaaggaatgc
     3961 cagcgaaccg ttggttccaa ggtttccacc ttttttggtc agggttgaac ggactgcgct
     4021 aatggttcgg ttgttattat cggtaaggca ctcaataaag atggcaatgc cccccgggcc
     4081 atagccctca aaggtaagtt cctgaaggtt ttcaggatct ttctcagcct tagtaatagc
     4141 ccttaggata ttttccttgg gcatgtttac ccctttggcg ttgttaatgg caagccttaa
     4201 tcgtgggtta ccctcagggt cagcccctcc ctcctttacg gcaacggata tttccttgat
     4261 tatcctagag aattcctttg aacgcttggc atcaagagcc cctttcttgc gtttgatggt
     4321 tgaccattta ttgtgtccac tcatagtttt ttatttttgt cacaggtaaa gtgcaaagtt
     4381 agctacaaac ttgtgcttaa caaaaatatt tagcctctat tgatttgtat cactttatac
     4441 acttaaagca aaatattcat aaattcttac ctttgcaaaa aaataagatt tccaatggat
     4501 tttaatgccg aagatgccaa catttttgaa agtgcgctga gcgatgatag tagccgccct
     4561 aaggtttaca ttgaaaccta tggctgccag atgaatgtaa acgatagcga ggttgtggca
     4621 tcaattctaa tagccaatgg atacgcaata actagcaaca ttaacgaagc cgatgttatc
     4681 ctgataaaca cctgctccat tcgtgagaat gctgaaacac gtgtgtttgg acgaattgat
     4741 ttatttgggc aagtaaagaa gtcaaagcca tcggtattgg ttggggtatt gggctgtatg
     4801 gctgaacggc taaaggatca gcttttagaa gaaaagaaag tagttgattt ggtggttggc
     4861 cccgatgctt accgtgaact accggttctg ctgcgtgctg ccgaggatgg tcaaaagggc
     4921 atcaatgttt tactctcccg ggaagaaacc tatgccgata ttagccctgt tcggctcgat
     4981 aaaaatggag tttcagcctt tgtttctatc atgcgtggat gcaacaacat gtgttcctac
     5041 tgtgttgtac cttacacacg tggagcagag cgaagccgcg atccccatac cattgtaagg
     5101 gaggttcagg aattagttga tgctggttac cgcgaggtta ctctgcttgg ccaaaatgta
     5161 aactcctacc actgggagga gcaaaaaggc ctaatcaagc acaaggtaaa cttttccgat
     5221 ttgctcgaaa tggttgccct tgtcgaccca cggttaaggg ttcgcttttc cacctcacac
     5281 cccaaggatt taaccaatga ggtgctttac accatggcca tgtacgagaa catttgcaac
     5341 catattcatc tccccgttca atctggctcc acccgcattc ttcagctaat gaaccgcaag
     5401 tacacccggg aggattacct cgacagaata aaagctatac gctccattat ccccgattgc
     5461 tctatatcaa ccgatattat agctggtttc tgcacggaaa ccgaggtcga tcacgatatg
     5521 accctcagca ttatgcgtga agtgggcttt gactttgcat acatgttcaa gtactccgag
     5581 agacctaaca caaaggctgc tcgccagcta aaggacgatg tgcctgagga ggttaaaact
     5641 cgcaggttaa tggaaatcat tgaattacaa ggcaaacttt ccgaacaaag caagaagagc
     5701 gatttaggca aacgctttga agtgcttatt gaaggagaat ccaaaaagtc aaaagatgaa
     5761 ttttttggcc gaaccagcca gaataaggtt gttgtattcc caaaggagaa ccacaaaatt
     5821 ggagagtttg tatttgtaga gataactgac tgcactccgg caacattaat tggtcgcatt
     5881 gtgcaggagt agactattta atacgcttta gccttgccat gtaaaaccca tcaaaccctg
     5941 tttgggatgg ggataggttt ttttcctctt ccagtacaaa gcggccattc atttgctcca
     6001 ggaaccattt aacctgctct tcattctcag agggtagaat actacaggta gcataaacca
     6061 tacagccccc aaccttaagc attgaccaat acttttcaag tatttgtctt tgagtttttc
     6121 gtaactcagc aatgttttca ggagtcaacc gccatttgga gtcagggtta cgcttaagca
     6181 ctcccaagcc cgagcatggg gcgtcaatca gcaacctatc ggcactatcc tgtaaacgtt
     6241 ttatcgtttt tgttgtttct atggctttgg tctcaacatt tgtaacccca gcctttcttg
     6301 acctcctttt aagctcttca agtttccatg gggcagtatc aagggcaata attcttccct
     6361 gattattcat gaggcttgcc aggtgcaatg tttttccacc attcccggca caagcatcaa
     6421 taacacgcat gcccggttcc acatttaaaa atggaactac ttgctgcgat gaaaaatcct
     6481 gctgctcaaa cattccaagc ttaaatgcct cggatttaaa aatgttggcc gattcggtta
     6541 gcagtatggc gtgggtgttg ggaataattt ttgttttata cccctcacgt tttaaaaaat
     6601 tattaagctc ttgaacgttc gtttttaaaa ggtttgttct taggactatt ggggctttat
     6661 gatttaagct atgaatctcc tttggccatt tttcgcccag ttccttaaaa cctagttcat
     6721 cgagccaatc gggaatagac tcctttattg ccctattatt tgaaagctga tttagtttgt
     6781 tctgaagctt ctttttaaaa tgttttggaa tcttacgctc ctctggaaat ccaaagggtg
     6841 ttagggttgt ataaatggct acaatgttat gagtgtgtga tccgaatttt ggaaattcac
     6901 tatcgtgcag ctcgttcagc aacctccacc atcgtgttgt ttcctccaca ataactgcaa
     6961 actggtactt atcaaagggt tcccataatt tattttgagc aatccagtac gatattacct
     7021 ggtctaaatt tcgattttgg taaacaacct caccaagggc gcgacctgcc gtttcaaata
     7081 cacttcccat aatataaaaa ttaaaggccg caatttacgg cctttttatc gataaagaat
     7141 gaaatttaga atttattttg gtactcaggc tttagggttc catcctcatt gaaccattct
     7201 gccaaaccgt agtaggctat tttatcgtcc ggacgggtat acttcaaaat ctttttttcg
     7261 tattttgtta gttttgtaag attggttgct acggccatac gggttctgtc cttttcaacc
     7321 tcgggcagct gaagtttttc aagagctccc actgtaaggt cctttgcctg gagcaattta
     7381 tcgttcgatt taaaaatatc gataattaaa ttgttccatt taattttttc gccaccaacg
     7441 gttggtttta aaccctttcg taacgatttt ggtttccgtc cccttttctt aggaggcgat
     7501 gttttaactt ttttcttggc tattttttta cgaccttttt ttggcttttc agtttttgtt
     7561 tctgaggcta cttttggttt ccttcctctt ttcttgggct cttttgtttg ggcactttta
     7621 tcggcctcgg ctgtaatttt cttgggacgt cccctttttt tgggggcagt gggttctgtg
     7681 gcttctttta cttttggttt gcgacctctt tttttaggag cggcaacttc cttttcatcg
     7741 cccaattcgc ccttttcaat ttttcgcaaa attgatttta aactatcaat tcgtcgttgt
     7801 gccctatcaa tctctgattg gtaaagatct tttagatctt caatctcctc ttttgagagt
     7861 gtgatttttt tcatggttgg ttagtttagt gttttactcc tgatttctaa ttattttgaa
     7921 ttgcaattta aaaacatttt attttaaaat gcaaatattt acaaaaaatt gattaaaaaa
     7981 ttttatttaa ttattttttc aatgtccata aaaaccagaa aatgaaatat ataggataag
     8041 tcaaccttca attgagcaaa ttattatttg aaaataaaat ttagttggat ttaactactt
     8101 tcccttcctt attaaagtac tgctaatgtt atatacgacc aatattaata ccaaatacca
     8161 tgaattaaca aattcaaatg ggctaatttt agaggaatga gtcggaatga acctgaatga
     8221 ttcggaatga ttcggaataa gctgaatatg atggagttag aggaagttag aggaatagta
     8281 aaaagtgaac ggctaaacca aatgaccatg aattaacaaa ttcaaccggg ctaattttga
     8341 ggaatgagtc ggaataaacc tgaatgattc ggaatgattc ggaataattt gaatatgatg
     8401 gagttagagg aagttagagg aatagtaaaa agtgaacggc taaaccaaat gaccatgaat
     8461 taacaaattc aaccgggcta ataaaaaatg tggatacaga aacattttta gaggaagttg
     8521 gttatatgaa ttttcaattg acgaatcgac taacaaaatt taatcattcc gaaatcccgg
     8581 aattaaaaat tttgataagt ctggattact tcgataactc aatagaaaca agctgtttaa
     8641 atgttttatg gtgtttctat tgactaaact cggataatat aatccataaa gtagttaatg
     8701 gttaatgtag aggaattgat aaaatatcat gtttacgcac cgtttagcgc gaataatata
     8761 tttatttatt gaggtttcta taaaaattcc tgaagctttt gaaccatatt ttttgaacct
     8821 acataaagag gaactctttg gtgaagcgat tctggctgaa tgtcgagaat tctattttta
     8881 ccatcggtgg caatgcctcc ggcttgttcg gccaggaaag caatagggtt gcactcgtaa
     8941 attaagcgca acttgccttt tggtgccgat gcggttggcg gatacatgaa aatacctccc
     9001 ttaaggagat tcctgtgaaa gtcagaaacc aacgaaccga tataccttgc agagtaaggt
     9061 ctttttgtgg gcttatcttc ctgcttacaa tactcaatgt attttttaac accctcaggg
     9121 aagtggttat agttaccttc gtttatggag tatattgccc catcgggggg cgtaactata
     9181 ttaaggtgcg ataggcaaaa ctcacctatg gaggggtcaa gggtgaaacc gtaaactcca
     9241 cggcctgtgg tataaacaag catagttgac gatccgtata ttacgtaccc tgcggctacc
     9301 tgtttattgc caggctgcag gaaatcctcc atggtaggtt ttgttccacg ggggcttatt
     9361 cgtcggtaga tactaaaaac agtacctatc gatacattta catcaatatt cgatgatcca
     9421 tcgagcggat ccatacaaaa gatataattg ccatccttgc taagttcatc gtcccaggta
     9481 attatttctt gatcttcttc cgatgctaca ccgcagcact ccctgctgtt ttttagcgct
     9541 tgtacaaatt gccagttggc taacaggtcg agttttttct gttcctcgcc ttgcacgttt
     9601 atgttacctg cctcgcccag aacgtcaaca agtccagctt tgttaacctt cttgtttaca
     9661 agttttgctg caactcccac ttggtaaagc aatcgggtga attcccctgt agcatagggg
     9721 aaatcggctt gcctctcaat gataaactgt gttagtgttt ttacattgta tccgttcatg
     9781 atagttatta attctaattt gtgagtaaaa ttagccattt tttccgttgc aaaggcatgg
     9841 catgacaaaa aaaaatcaaa tttaaaacgt ttgcgatgct attccttaac cagcaatcgc
     9901 taaaaaaagt aactcgcatg ttaagagtat atacttagct tttttgtaag tttgagaaat
     9961 tttttggcaa tgaaacccat tcgtgtcttt aagtttggcg gagcctcggt taagtcggcc
    10021 ggtgctatta gaaatttggc caatattgta aaacgatttt ccactgaaaa cttggtgatt
    10081 gttgtttcgg ctatggctaa aacaaccaat gctctggagg aggttttaat gtactggatg
    10141 aatgccgatt taacggcaat gaatactaaa ctcaaggaga ttaagcaatt ccacctaaac
    10201 gaggcactgg atttggttga gggggattcc aaccacttgc ttattgctga gctgcaggcc
    10261 ctgttcaacc agttagaaac ccagtttggc cagactccta caaagcgata cgatttccat
    10321 tacgatcagg ttgtgtcgtt cggcgagctc ttctctaccc ggattgtcag ccactacctt
    10381 aattttatag gcattgccaa tactttagtg gatgcaaggc gatgccttag gtccgataca
    10441 tcttaccgtg aaggagtaat tgatgaaaca acatcgcggc tattgtgcca gcgtgagttc
    10501 gatttctcat cggcaaatat ctttttaacc cagggcttta tagcaggaac cgaggtggga
    10561 acaacaacca cgctagggcg tgagggttcc gattacacgg ctgccattct tgctaatctt
    10621 ctcgatgctc aggacgtaac catttggaaa gatgttgcag gtgtgctaag cgccgatcct
    10681 aagattttca gcaatgcggt taagcttagc tttgtgtcgt atcaagaagc tattgagctt
    10741 gcctattgcg gagcacaaat tattcaccca aaaacaataa aacccataca gaataaaaag
    10801 ataccccttt atgtaaaatc gtttatggag cccgatgctc agggaactat cattgcccat
    10861 tccgatactc ccgttaagct accccctgtg ttggtactca aaaggaatca ggttctaata
    10921 accctttcgc cccgcgattt ttcgtttgta attgaggatt gcctcagtaa gatatttgcg
    10981 ttgctatata aacatcgcat taaggttaat atggtgcata actcagccat aaactttacc
    11041 gtttgcgttg acaacgataa gctccggctt ggtgatgcac tggaagaaat gaaacacgaa
    11101 tttgccgttc gttacaacag taaccttgaa ctgttaacca ttcggcatta cacgccggaa
    11161 ataattgaga attttattgg caatcgggtt gtttatctgc aacaaagaac cagaagcact
    11221 gcacgctttg ttatggacgc cagctagcgc ctagtatttt accgtgaata gcgcgctata
    11281 acctttaaag gttccttgtt tgctatactc ggatttacca aaggttatgg agagtatctt
    11341 tttatggagg tgcccttgtt tatcagtaaa ccactgttcc gtaaaaagta gtttgccaat
    11401 ttgtgaagca tctattttat ggtcttcaat tatctttcta acctcttcaa cggtcaatgg
    11461 ttcgttggtt gtatagtcaa aaacctttaa tttaccgcta agtatacctt taaatgtgcc
    11521 atcaataaaa cttttgtggt tccggtagcc ttgaagcctt tcactttccc aaatatcatt
    11581 ttcggatgct tgaactacaa cctcatactt aatgctatcg gcaattaagg ttaagtcctg
    11641 ctcctttcct gtaatgtcaa caattacatt tttaggctgc ggattgctgc acgactcaaa
    11701 cacaatagca agcaaaatca acttaaactt aatgttcatt ggtttagggg ttaaaggtta
    11761 gagcttttcc cttaatggtt tcattgaaaa ctcctttact gtaaacaatt ctcccgttta
    11821 aaattgtcat ttcaaccgtg tgtgaaagcg tttctccctc caggggcgac cagccacact
    11881 tatagtacag gttctccttg gcaacagcct gtgatttatt tggattaaca agcacaaggt
    11941 cggcaaagta tccctcgcgt ataaagcctc gtttttcaat cctgaaaaga gtggctgggg
    12001 agtgacacat cttttcaact acaagctcag gcgtaaaata ccctttccta cttagctcaa
    12061 gcatagctaa cagcgagtgc tgaaccatag ggcctcccga tgctgaacta gtgtacacat
    12121 tatctttttc actaatcagg tgtggggcat gatcggtggc cactacatcg agcaggttgt
    12181 tttttaaacc ctggataagg gctaaccggt cggtttcagt ttttacgctt gggttccact
    12241 taatacgcca gcccaacctg tcgtagtcat tattattaaa ccataaatgg tgaacgcaaa
    12301 cctctgcggt aatctttttc tcggccaagg gtatgctgtt gttgaaaagt tcaagctcgt
    12361 tggctgttga aagatgaagg atatgtaggc gagcgccatg tctttttgcc agtctaactg
    12421 cttttgccga tgataggtag caagcctcag cgctccttat gcttgcgtga tattttatat
    12481 cgatgttgtt tccgtataac ccttggtatt tttcaaggtt tttgcggata gtatcctcgt
    12541 cttcgcagtg tgtggtaatt atagtaggtg agaacctaaa gattttttca agcgcatcct
    12601 cattatcaac cagcatatta ccggttgatg aacccataaa aacctttaat ccgcaaacat
    12661 ttctagggtc aacctttgta atctcatcaa tattatcgtt ggtggcgcct aaatagaacg
    12721 agtaattggc taacgatttt tggctagcaa tcttaaattt atcgtggagt agttcaacgg
    12781 tagtagtttg tggtttagta ttaggcatct ccataaatga tgtaacacca ccagcaacag
    12841 ccgctttcga ttcggtgtaa atatcaccct tatgggtaag gcctggctca cggaagtgca
    12901 cctgatcgtc aataacgcca ggtaaaagcc ataagccctc agcatttatt atctcctcgg
    12961 gcaagaactt atctacactt gatggggcgg tactaatatg ctcaatggtt tcaccattaa
    13021 caattaggtt tcccttaaag gttttgccct cgttaataat ggtagcattt ataatggcta
    13081 gtcggcccat ttatggttat ttagtttgaa gcatgaagtt ttttgtacga aacaaaaagg
    13141 cttttaagtt tcatgttaac cacgccccac aatgcctcat tgaaaatacc tccactcatt
    13201 tttgaagcac caaattttct gtcggtaaat attataggta cttcaactat gttgaatcca
    13261 agcttccaca cggtgaattt catttcaatc tgaaaaccgt aacctatatg ctttatcttg
    13321 tcaaaattga tggcctttag cacttcggct ttataacatt taaaacctgc agtagtatcc
    13381 atcactttca ttcctgttac catccgtaca tacttagagg caaagtacga cataagcaca
    13441 cgtccaatgg gccagttcac aacatttacc ccgttaacat acctcgagcc aatagccaga
    13501 tcggctccgt taacgcaggc attgtacagg ttaattaaat cgtcggggtt gtgggaaaaa
    13561 tccgcatcca tctcaaaaat atacttatag ccattggcta gagcccattt aaatccagtg
    13621 atatatgctg tccccaatcc cagtttccct gagcgttcaa tcataaaaag cctatcgggg
    13681 tattcctttt gtttttcctt aactatactg gctgtgccat cgggtgagcc atcgtcaaca
    13741 ataagaagat ggaagttccc ttcaagcgac attaccttgc caatcattcg ggaaatgttc
    13801 tcactttcgt tgtaggtagg gataattacg agtttctctt taaacgacat ctgaatatga
    13861 ttgtagtttg taatactata atttataaac tattgtgcga agatagcgtt ttttacccaa
    13921 aaactaacta tctcaagcat gaaagcaaaa tttttggttt gataatcagg ggataggctt
    13981 tcatgaaaca ctctttaaca aaaatattct gtatagaccc ttgcgtaatt aaaaacgctt
    14041 ttctactttt gcacccgctt tcggagggga gcgc
//
LOCUS       DAOOWJ010000070        13711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4500_length_13711_cov_9.049136, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000070 DAOOWJ010000000
VERSION     DAOOWJ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 13711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13711
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_13711_cov_9.049136"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="PLW22_07830"
     CDS             complement(<1..1586)
                     /locus_tag="PLW22_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU74653.1"
                     /translation="MSEEILKALMQLFAIIAKQDEGVELKERDYVVNFLTQQLNDEAV
                     KEYIRLFDEHAGLLDKEDNAENDEEKGKRKLTSVKDSVRILGICKKINKTLTQKQKIV
                     VLVRLYELINADRKFTEQRMAIINTVAEVFKLTKEEISDIENFVIKNSPEELDIPSIL
                     TIHDKPFTGKNCKHIGTEALDGYIYILQIKSEDLYFLRYTGNEDIYLNGLPIHNRRIY
                     LFASGSSLKLPKGKPVYYTDVVSHFLADSTSIRISYKVENVGFQFKTGDIGLRNISFS
                     EGHGKLVGIMGASGAGKTTLLNVLAGIEKPTTGRVIVNGYDLHNEKEKLEGVIGVIPQ
                     DDLLIEELTVFENLYYNAKLCFKDKTEEELVALVDKTLANLGLLERKNLKVGTPLNKM
                     ISGGQRKRLNIALELIREPSILFVDEPTSGLSSRDSENVMDLLRELTLKGKLIFVVIH
                     QPSSDIYKMFDNMVILDTGGYMIYYGNPVEAVMYFKRLDMQINSDVGECPVCGNVNPE
                     LIFNIIEARVVDEFGRYTPIR"
     gene            complement(1620..2003)
                     /locus_tag="PLW22_07835"
     CDS             complement(1620..2003)
                     /locus_tag="PLW22_07835"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU74654.1"
                     /translation="METIKIMGTDDTPTVILDAQNEIFEISGRSLPEDVTAFYDPILQ
                     WLDDYSAAPKPKTIFTFKLVYFNTASSKLLLDILMKLEQLKEAGHDVLVRWYYPEDDE
                     DMQEAGEEYADIVDVPFEQIGYTLS"
     gene            complement(2014..2589)
                     /locus_tag="PLW22_07840"
     CDS             complement(2014..2589)
                     /locus_tag="PLW22_07840"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOU74655.1"
                     /translation="MDRKNIKGFLEFVYDFYKSMKAHEITLVYEGEITHQITKAFTSL
                     TESNMAKEEESNSVQKKVFHVMVECLQNISKHADSFGSDDFLFAGRGIFMVSKGDSEY
                     HVTTGNVIENSKIEELSKILDHINSLDKEGLKQLYKTQMREGRLSEKGGAGLGFIDIA
                     RKTGRKLEYHFLPIDEETHFFVLTSTISRSE"
     gene            complement(2629..5241)
                     /locus_tag="PLW22_07845"
     CDS             complement(2629..5241)
                     /locus_tag="PLW22_07845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU74656.1"
                     /translation="MISFGIISTTDSKQVIRQRVTQAFLVSLMFPVIAWIVDILSRGI
                     PFNLTSIADIHATNPIHWIIDLAPFVIAYVTWLQLTRHYRRILLLEKELRQRDEDIEK
                     NARFAQRIGEGDYNAPFQVGGDDDILGKSLVIMRDNLLASQKKEAEQNWITKGKDEIS
                     HILRLHHNIDELSYDVLVKLINYIRTIQGAIYLYDEEKQKLVNLATYAYNRRKYIKQE
                     FRIGEGLIGECAYEREFIYRTEVPDNYVTITSGILGDRKPKSLLLVPLITDEKLEGVL
                     EFASLDDEIPELTIRFLKEVGEIIARTIFNLRINQKTEKLLQEAQQMAQELKENEEEL
                     RQNAEEMKITQEELEISNAKLEAKIQEVENAQKRLHSLLENASEIISIYNSEKQLTYI
                     SPSVTKILGYTPQEMMSGKDIDRLTRKGEAAFNEMFEQLLENPRIAITIQYTFMKKDG
                     EKIFLEVTGRNLLDDPAIGGIIINSRDITERKRAEKEERMRSKMQSLSENSPDIIMRV
                     NPAGQFFYANPMVETYLGIDYRELVNQTITALDGIPVLSNFIREAIKTIKVEKEKYEG
                     EITFTTNRGETVMHIVGIPEFNENELETILFVAHDITEQKQTEREIQEKNRKITESIN
                     YAQRIQTSILPNNRIIRQYLPKSFIYYHPRDVVSGDFPWFFIKDDFIYIAAVDCTGHG
                     VPGALLSFIGYFTLNNVVDHDSSYTAGQILDHLHFGVRKTLKQDRPDADARDGMDIAF
                     CKINPKNKELQYAGAHRPLYLVRNGELTEYKGDRKAIGGIPHPKKAEEPFTNYIIEYK
                     PGDKVFFFSDGIVDQVGGPEKKKFGAQSIRDIIMNNLKTPIHELNDVFAKALTDYQGE
                     NKQVDDILLIGIEF"
     gene            complement(5257..6036)
                     /locus_tag="PLW22_07850"
     CDS             complement(5257..6036)
                     /locus_tag="PLW22_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU74657.1"
                     /translation="MKPINPTNRFVPVIVLVLLLATSLLVFSLIYNTALETGQKISPW
                     IIGLCLLPFLFGLWFYFIVVKQRNLIDGLQAQIELLKAQVESFMKSQKKETTQEIRKE
                     TLDYKKLLEKIIPNLPTDDIVKYGEALLANVAKTAEIVQGQLYLKNSQTDVFSFKAGY
                     AFFSETTPPEYREGETLAGQVAKNQKLINIDNIPEGYITILSGLGKGSPKHLIIAPII
                     STENTTIGIIELASFKPFTSEHEELFSQLGRKLGEILSVNQ"
     gene            complement(6041..6601)
                     /locus_tag="PLW22_07855"
     CDS             complement(6041..6601)
                     /locus_tag="PLW22_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOU74658.1"
                     /translation="MYKAVIIGGSAGSFQVITRILHALPPNYPLPVLLCLHRLKHVRS
                     GFVEALSIKSGIQIIEPNDKEQIKPGKAYLAPANYHMYVELGNKIALSTEEPVNHSRP
                     SIDLSFITAAQVYREKLVGIILSGANRDGAYGLKKIKDLGGLAIVQNPDECQVRTMTE
                     ASLKMTPVDFVFNTNEIIKFLQNIKV"
     gene            complement(6607..7443)
                     /locus_tag="PLW22_07860"
     CDS             complement(6607..7443)
                     /locus_tag="PLW22_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOU74659.1"
                     /translation="MTIRYEIGIVDTRNVIKTLLDDYGFDFRDYALTSLKRRLEYIIS
                     LYALRDAEGLIAKLKSSKDFLDQFLYDITPETTEMFRDPSFWRILRDEIIPELVKSGS
                     SKPRIWIASFDSGEELYSLSILLKELNLLNEVNLYCNVLSDHTIQRITSGRLDPKDME
                     VNEANYQRFNEKGKYSSYYRTEANSIIFDVSLIKDVNFIKQNTLIDEDPGAPKLIIFR
                     NQAIYYNQLLSEKVFAKLANCLVAGGYLAIGVKETLENTNVSNKFTLYNEAERIYKRK
                     TN"
     gene            complement(7450..9822)
                     /locus_tag="PLW22_07865"
     CDS             complement(7450..9822)
                     /locus_tag="PLW22_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU74660.1"
                     /translation="MKAFANMKLAQKVQLAIIPLMVVLFAFAGLILNYFSRTRTINNA
                     VKEAYVYIDRLIDIANIVEQKTGKGLTSNDIFELKPYFNKPAFNKTDFPSLISRNGEY
                     VLHITRQGARLPMKTIQEIKNLKANEGFIYYQDITDDITENRILVYKYYKPYNAYFAI
                     SVSPTEVLEQVKANRRAMIAIVFLSSLISVIVVWLLLKPITKQLAHLSVRLKQLAQGE
                     IPPAVKVTDRSELGTLAESLNSLIDGLKSTTEFARQIEQNKLDFDFKPLSKNDQLGLA
                     LLQMRESLKRAQQEEAKRKKEDDIRTWINAGLAKFGDILRQNNNNLNLLADNVIQNLV
                     NYLDANQGGIFMFKEYESEKHLELLSAFAYNRKKFLQKSIMLGEGLVGTCAVEKQTIY
                     LKEIPSDYIAITSGLGEATPTSLLIVPLKLEDEIFGVIEIASFNEFAPYQIEFVEKVS
                     ESIASTLNSVKNNIRTRELLEQSQQQREEMAAQEEEMRQNMEEMQATQEEMQRKNLEL
                     EIITSAINQSLVSCSLNDDGFIIDSNLVFQEMTGYSKVELEGMLLESLIVDSQREEFS
                     KLWKSVLNGQSINTTLHLIGNNGRERFLLASLTPGFDPMGLLIKILLMGHDITESKKL
                     ELMTQQQAKEIEKNIKRIKEEQEISNLRQREMETLLKALDQNCLVTIIEPDGLITYIN
                     NKNVEVLGDKKEDIENRFIQDIDFTAKNKPKEFERFWKSLHKGIKQTREFSLDVKGET
                     RWVLENYTPIMDESGNLYKIINIALDITENKRKEMELNAVIEQLKQQLKK"
     gene            complement(9831..10685)
                     /locus_tag="PLW22_07870"
     CDS             complement(9831..10685)
                     /locus_tag="PLW22_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOU74661.1"
                     /translation="MPSQRMQVTDQDLQYFVNTIKSVSKYDFSEYSEKSLKRRLQKIL
                     EDYNLDLTSLLTSLRLDRDFLEKVVKEVTVNTTELFRDPPVWHLLRSRILPRFKNNKT
                     INIWHAGCSTGQEVYSMMILLNEMEMLDKAKIFATDLNSDVLDAAKQGVYKYRFNLGY
                     LDNFDKVIKQNPLNFEEYNDVPYEKYFDIDKVRDTITMKKFLTERPIFRKHDLVVDGN
                     IFFAKFDLILCRNVIIYFNYSLQNKVFELFHNNLYPKGILLLGMHETILGPWATRFER
                     LGQAYIKK"
     gene            complement(10829..11887)
                     /locus_tag="PLW22_07875"
     CDS             complement(10829..11887)
                     /locus_tag="PLW22_07875"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU74662.1"
                     /translation="MIKNTLRLGFITIAFAFIFAILFMVYSTITFYNPEREITLQVSL
                     NPDTVAISTPYQLITWNIGYAGLGDNMDFFYDGGKQTRDTKARTLENLDSIKKFILQN
                     RTDFIFLQEVDLASRRSYNTNQLQIIGQAVNYFTFFATNYQVSFVPVPLKNPMGNVNS
                     GIMTLSRFTPMNAVRVQLPGLSPWPNRVFNLRRCLLVTRFPVSNGREFVLINTHNSAF
                     ENDSLKMAEMSFIRQVALSEYEKGNYVIIGGDWNQLPPNFPPDRFGNAYETKAFKPLS
                     IESSFMPGGWQWAFDPSKPTNRYLDRPFSNQCRTVILDFFLLSPNIHITDIATTDLKF
                     RHSDHNPVKLSFVLMPIQ"
     gene            11997..12701
                     /gene="purQ"
                     /locus_tag="PLW22_07880"
     CDS             11997..12701
                     /gene="purQ"
                     /locus_tag="PLW22_07880"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOU74663.1"
                     /translation="MKFGVVVFPGSNCDQDTVYVIKEILEQEVVTLWHKETDFDPNID
                     VIILPGGFSYGDYLRSGALAKFSPIMNRVIDFANNGGYVFGICNGFQILCESGLLPGA
                     LLHNTSQKFICKNVHLVPDSKNTALTKYLPRDRALKVPIAHGEGRYYADKDTLAQMRI
                     NGQILFRYCDERGVVSESANPNGSVENIAGVCNDRHNVFGMMPHPERASDDELGNTDG
                     RLIFESMIKYIRELSN"
     gene            complement(12698..13411)
                     /locus_tag="PLW22_07885"
     CDS             complement(12698..13411)
                     /locus_tag="PLW22_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU74664.1"
                     /translation="MNYFYITGTSSGIGLALTRQVLSNDSSYVVGYSRRNVVSHHRYK
                     HIQIDFSQQGAAREVEIPFFPNAKHVVLINNAGILGDVKPIGKIDNEQLEKTFMVNTV
                     SPAILTNRLLALYLPSAKLITVVNISSGAARHAVESWSGYCASKAAIDMFSMVLSAEQ
                     KLLGDSKVRVISLAPGIVDTPMQDSVRKVDPKYFPQSERFHQYKQKGMLASTEQVAKK
                     IVELIGNPPNDPIIDLRML"
     gene            complement(13485..>13711)
                     /locus_tag="PLW22_07890"
     CDS             complement(13485..>13711)
                     /locus_tag="PLW22_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HOU74665.1"
                     /translation="VMWIMMLVSPTPGGSGFAEFVFTRYLSEFIPVDAALIVPVSVAM
                     ALLWRLVTYYPYLFLGSIIFPRWVRRHFIK"
BASE COUNT         3615 a         2823 c         3083 g         4190 t
ORIGIN      
        1 cgtatggggg tgtaacggcc aaattcgtca actacgcgtg cctcaatgat gttgaagata
       61 agttccgggt ttacattacc acaaacaggg cattcgccaa catcgctgtt aatctgcata
      121 tcaagacgtt tgaaatacat cacggcctca acagggttgc cgtagtaaat catgtatccg
      181 cctgtatcca gaataaccat gttatcgaac attttgtaaa tgtccgatga gggttgatga
      241 ataaccacaa atatgagttt ccccttgagc gtaagctcac ggagtaggtc cattacgttt
      301 tcagagtcgc gcgacgaaag ccccgatgta ggctcatcaa caaaaaggat ggagggctcg
      361 cgaattagct caagggctat gtttaacctt ttacgctgac cgcccgatat catcttgttg
      421 agcggggttc caaccttgag gtttttacgc tctaaaaggc caagatttgc aagggtttta
      481 tccacaaggg caaccagttc ctcctccgtt ttatccttaa agcaaagctt tgcgttgtag
      541 tataggtttt caaatacggt taactcctca atgagcaaat cgtcttgcgg gataacgcca
      601 ataacacctt ctagtttctc cttttcattg tgaaggtcgt agccgttaac aataactcgg
      661 cctgtggtag gcttttcgat accggcaagt acgtttagca gtgtggtttt accagcacca
      721 ctggctccca tgataccaac cagcttacca tgcccctcgg aaaaggaaat gtttcttaat
      781 ccgatatcgc ctgttttaaa ctgaaaacca acattttcta ctttgtagga aatgcggatt
      841 gatgtggagt cggccaggaa gtgggaaacc acatcggtgt aataaaccgg cttcccttta
      901 ggtagtttaa gtgaactccc gctggcaaaa agatagatgc ggcggttgtg aatgggtaat
      961 ccattaaggt aaatatcctc attgcctgta taccggagga agtaaaggtc ctcgcttttt
     1021 atttggagaa tatatatgta accatccagg gcttcggtgc caatgtgctt gcagtttttg
     1081 cccgtaaagg gtttatcgtg aatggttaaa atacttggaa tgtctaactc ttcgggtgaa
     1141 ttttttataa caaaattttc aatatccgat atttcttcct tggtaagctt gaatacttcg
     1201 gctacggtat tgataatagc cattcgttgt tcagtaaact tgcggtcggc attgataagc
     1261 tcgtacaaac gaaccagaac aactattttt tgtttctggg taagagtttt gttgattttc
     1321 ttacaaatac ccagaatacg aacggaatcc tttaccgaag taagttttcg tttgcccttt
     1381 tcctcatcgt tttcggcatt gtcttctttg tcgagcaggc ctgcatgctc atcaaagagt
     1441 ctgatgtatt cctttactgc ctcatcgttg agttgctggg tgaggaaatt aacaacatag
     1501 tcacgctcct tgagctcaac accttcgtcc tgctttgcaa tgattgcaaa gagttgcatt
     1561 aacgctttga gaatctcctc actcattctt cttcacttgg gcaaaaaacg gaaaagaacc
     1621 taactcaagg tgtagccaat ctgttcaaaa ggaacatcaa ctatatcggc gtactcctcg
     1681 ccagcttcct gcatatcctc gtcgtcctca gggtagtacc aacgcacaag tacgtcgtga
     1741 cccgcctcct tgagctgttc aagtttcatc agaatatcga ggagcagctt agagctggcc
     1801 gtgttaaagt acaccagctt aaaggtaaag atggttttgg gttttggcgc tgctgagtag
     1861 tcatcgagcc actgcagaat tggatcgtag aatgcagtaa cgtcctcggg caaggaacga
     1921 cccgagatct cgaagatttc gttttgagca tccagaatta cggttggggt atcatcggtc
     1981 cccattattt ttatggtttc catatcggta ctgttattct gatcttgaaa tggttgaagt
     2041 taaaacaaag aaatgggttt cctcgtcgat ggggaggaag tggtactcaa gcttccttcc
     2101 ggtttttcgg gctatgtcaa tgaaacccaa tccggctcca cccttctcgg aaaggcggcc
     2161 ttcgcgcatc tgggttttgt agagttgctt caaaccctcc ttgtcgagcg agttaatgtg
     2221 atcaaggatt tttgagagct cctcaatctt ggagttttca atcacattgc cagtagtaac
     2281 gtggtattcc gaatcgcctt tgctaaccat gaaaatacca cggccagcaa agagaaaatc
     2341 atcggagccg aaactatcgg catgtttgct gatgttctgg aggcattcca ccattacatg
     2401 gaaaaccttt ttctgaacgg agttcgactc ctcctccttg gccatatttg actcggtaag
     2461 ggaggtgaag gctttggtta tttgatgggt tatttcaccc tcgtaaacga gagtaatttc
     2521 atgagccttc atcgacttgt aaaagtcgta aacgaactca agaaaccctt taatattttt
     2581 tcgatccata gcccgttgag ttatatgagt tacaaatata ttacattttt aaaattcaat
     2641 accaattaaa agtatatcgt caacctgttt attttcaccc tggtaatcgg taagtgcttt
     2701 tgcaaacacg tcgttaagtt cgtgaatcgg tgtttttagg ttgttcataa tgatatcacg
     2761 aatactttga gctccaaact ttttcttttc agggccacca acctggtcaa ctataccatc
     2821 ggaaaagaag aaaaccttgt cgcccggttt atactctatg atatagttgg taaatggttc
     2881 ttcggccttt ttgggatgag gaataccgcc aattgctttg cggtcgccct tgtattcagt
     2941 tagttctcca ttccgtacaa ggtaaagcgg acggtgagcc cctgcatact ggagttcctt
     3001 gtttttgggg ttgattttgc aaaaagcaat atccatacca tcgcgggcat cggcatcggg
     3061 gcgatcctgc ttaagggttt tacgaacccc aaaatgtaga tggtcaagta tttgaccagc
     3121 tgtgtacgat gagtcgtggt caaccacgtt gttcagtgta aagtaaccaa taaaggagag
     3181 cagagcgcct ggaaccccat ggccagtaca gtcaacagca gctatgtaaa tgaaatcgtc
     3241 ctttatgaag aaccagggga agtcgccgct aacaacatct ctggggtggt agtaaatgaa
     3301 cgatttgggc aggtactgcc taatgattct gttatttggt aggattgatg tttgaatacg
     3361 ctgggcgtag ttaatacttt cggtgatttt gcggttcttt tcctgaattt ccctttcggt
     3421 ttgcttttgc tcagttatat cgtgggcaac aaacagtata gtttcaagct cgttctcatt
     3481 gaattcaggg atgccaacaa tgtgcattac cgtttcgccc cggttggtgg taaaagtaat
     3541 ttcgccttcg tacttttcct tttcaacctt aatggttttt atggcttcgc gaataaagtt
     3601 cgaaagcact ggaataccgt caagcgcggt tattgtttgg ttaaccaatt ccctgtagtc
     3661 aattccaagg taggtttcca ccatggggtt tgcatagaag aattgcccgg ctgggtttac
     3721 gcgcatgata atatcggggg agttctcgga aagcgattgc attttgctac gcatgcgctc
     3781 ctctttctcg gcgcgtttac gttcagtaat atcgcgtgag ttgatgatta taccaccaat
     3841 ggcagggtcg tcgagcaaat tacgaccggt tacttcaagg aaaatctttt caccatcctt
     3901 tttcataaag gtgtactgga tggtgatagc aatacgggga ttctccagga gctgctcaaa
     3961 catttcgtta aaagcggctt cgccctttcg ggttaggcga tcgatatcct tgccgctcat
     4021 catttcctgc ggggtataac ccaaaatttt ggttaccgag gggcttatgt aggttagctg
     4081 tttctcgcta ttatatatcg atataatttc cgatgcgttt tcaaggaggg agtgtaaacg
     4141 tttttgggca ttttcaactt cctggatttt agcttcgagc ttagcattcg atatctcaag
     4201 ttcttcttga gttatcttca tctcctcggc attctggcga agttcttcct cgttctcctt
     4261 aagttcttga gccatttgct gggcttcctg cagtaatttt tcagtttttt ggtttatgcg
     4321 caggttaaag atggtgcggg ctattatttc acccacctct ttcaggaatc gaatagtcag
     4381 ctcaggtatc tcatcgtcga gcgaggcaaa ttctaaaacc ccctcaagtt tctcatcggt
     4441 gataaggggt acaagcagta aacttttggg tttacgatcg cccaaaatac ctgaggtaat
     4501 ggtgacataa ttatcgggta cctcagtccg gtaaataaat tcacgctcgt aagcgcactc
     4561 gcctataagt ccctcgccaa tacgaaactc ctgtttaatg tacttacggc ggttgtatgc
     4621 atacgtggca aggttaacaa gtttctgctt ctcttcatcg taaaggtaaa ttgccccctg
     4681 tatggttcgt atgtagttta tcagcttaac cagtacgtcg tacgaaagct catcaatatt
     4741 gtggtgcaag cgtaggatgt gcgatatttc atctttcccc ttggttatcc agttctgctc
     4801 agcctctttt ttctggcttg caagtaggtt gtcgcgcatg ataaccaacg actttccaag
     4861 gatatcatcg tcaccaccaa cctgaaaagg tgcattatag tcgccttcgc caatacgttg
     4921 ggcaaagcgt gcatttttct caatgtcctc atcgcgctgc cggagttcct tctccagcaa
     4981 aagaatccta cggtagtgtc gggtaagttg tagccacgtt acataggcaa tcacaaaggg
     5041 agccaaatca ataatccaat gaatgggatt ggtggcatgt atatctgcga tgctggtcaa
     5101 gttaaagggg attcctctgg ataggatatc aacaatccat gcaatcacgg ggaacataag
     5161 gctaaccagg aaggcttgag ttaccctttg ccggattact tgtttgctat cggtggtcga
     5221 tataattccg aatgaaatca tgccgcagtt tggttattat tgattgacac ttaatatctc
     5281 tccaagttta cgtccgagct gagaaaaaag ttcctcgtgt tcgctagtaa agggtttgaa
     5341 tgaggctagc tctattatcc caatagttgt attttcggta gagattattg gggcaataat
     5401 taaatgttta ggcgaaccct tacccaggcc ggatagtata gtaatgtaac cttcgggtat
     5461 attatcaatg tttataagtt tttggttttt ggccacctgt cctgcgaggg tttccccttc
     5521 ccggtattcg ggtggggtag tctcggaaaa gaaagcgtag ccagctttaa agctaaaaac
     5581 atcggtttgt gaatttttaa ggtatagttg cccctgaaca atttcagcgg ttttggcaac
     5641 attggcaagg agcgcctcgc catattttac aatatcgtca gtaggaaggt tagggatgat
     5701 tttttcaagc aattttttgt agtcaagggt ttcttttcgg atttcctgag tggtttcctt
     5761 tttctgcgat ttcatgaatg attcaacctg cgcttttagc aattcaattt gggcttgaag
     5821 gccatcgata aggtttcgtt gcttaactac aataaagtag aaccacaaac caaaaaggaa
     5881 ggggagtagg caaaggccta taatccaagg agatattttt tgcccggttt ccaatgccgt
     5941 gttatagatc aagctgaaaa ccaataggga agtggctagc agcaaaacca atactataac
     6001 aggtacaaat cggttagtcg ggttaattgg tttcatgttt ttatactttg atgttttgaa
     6061 gaaacttaat aatctcgtta gtattaaaaa caaagtcaac aggcgtcatc ttgagcgaag
     6121 cttcggtcat agttctaacc tggcattcat cagggttttg gacaatggct agcccaccta
     6181 agtcctttat cttttttagc ccgtatgcgc catcgcggtt tgctccggag agaataattc
     6241 ccactaattt ttcacggtaa acctgagcag cggtaataaa tgaaaggtcg atgctggggc
     6301 gtgaatggtt aaccggctcc tcggttgaaa gggctatttt atttcctaat tccacataca
     6361 tgtggtaatt agctggagcc aggtatgctt tgccgggttt gatctgttcc ttatcgtttg
     6421 gttcaataat ttgaatgccc gatttgatgg aaagggcctc aacaaatccg ctgcgaacat
     6481 gctttaacct atgcaggcaa agaaggacag gtagagggta gtttgggggc aaagcgtgta
     6541 gtattcgggt aataacctga aagcttcctg ctgatcctcc tatgataact gctttataca
     6601 taaaaactag ttggtttttc gcttgtaaat tctttcagcc tcgttgtaca gagtaaattt
     6661 attactaacg ttggtatttt ccaaagtttc tttaacccca attgctaaat aacccccagc
     6721 taccaggcag ttggcaagtt tggcgaaaac cttctcgctg agaagctggt tgtagtaaat
     6781 tgcctggtta cggaagatta taagctttgg tgcacccgga tcctcgtcaa ttaatgtatt
     6841 ttgcttgatg aagtttacat ccttaataag gctaacgtca aaaataatgc tgtttgcttc
     6901 ggttcggtag tacgacgagt acttcccttt ctcgttaaaa cgttggtagt ttgcctcatt
     6961 cacctccata tctttaggat ccaagcgacc cgatgtgatg cgctgtatgg tgtgatcgga
     7021 gagtacgttg cagtataaat ttacctcgtt aagtaaattc aattccttta gaaggattga
     7081 taacgagtat agctcttcgc ccgagtcgaa cgatgcaatc catatgcggg gttttgatga
     7141 gcccgactta accagttcag gaatgatttc atcccttaga attctccaga acgatggatc
     7201 gcggaacatt tcggtggttt caggggtaat atcgtaaagg aattggtcta aaaagtcctt
     7261 gctactcttt agcttggcaa taaggccctc ggcatcgcga agcgcgtaaa gcgatatgat
     7321 gtactccagc ctccgcttta gtgaggtaag ggcataatcg cggaaatcga agccataatc
     7381 atcgagcaac gttttgatta cgttccgggt gtcaacaatt cctatttcgt aacgtatggt
     7441 catacttcac tatttcttga gctgttgctt aagctgttca ataacagcgt ttagttccat
     7501 ttctttccgt ttattctcgg ttatatccag ggcaatattt attattttgt aaaggttacc
     7561 actttcatcc atgattgggg tgtagttttc cagcacccag cgggtttcgc cttttacatc
     7621 aagtgagaac tcacgagttt gtttgattcc cttatgaagc gatttccaaa aacgttcaaa
     7681 ttctttgggt ttatttttag cggtaaaatc aatatcctga atgaatcggt tctcaatgtc
     7741 ttccttttta tcgcccagca cctctacatt tttgttattg atgtaggtaa taaggccatc
     7801 gggctctatt atggtaacca ggcagttttg gtcaagagcc ttgagcaggg tttccatctc
     7861 gcgctggcgc aggttcgata tctcctgctc ttccttaatg cgcttgatat tcttttcaat
     7921 ttctttggct tgctgctggg tcatcaattc aagtttcttt gactcggtta tatcgtgccc
     7981 cataaggagt atcttgatta gcaatcccat gggatcgaaa ccgggtgtga gtgaagccag
     8041 caggaagcgt tccctgccat tattcccaat caggtgaagg gttgtattga ttgattgtcc
     8101 gttaaggaca gatttccata gcttgctaaa ctcttcgcgc tgtgagtcga caattaagct
     8161 ctcaagcagc attccctcaa gttcaacctt gctgtagccg gtcatctcct ggaataccag
     8221 gttggagtcg ataataaaac catcgtcgtt aagtgagcat gataccaacg attggttaat
     8281 ggctgaggtg atgatttcga gttcaaggtt cttacgctgc atttcctcct gggtggcctg
     8341 catttcctcc atgttctggc gcatctcttc ttcctgtgca gccatttcct cgcgttgctg
     8401 ttgcgattgt tcaagcagct cacgggttcg gatattattc ttaaccgagt tcagggttga
     8461 ggctatgctc tcgctaacct tttcaacaaa ctcaatttgg tatggggcaa actcattgaa
     8521 cgacgctatc tctataacac caaaaatctc atcctcaagc ttaaggggaa caatgagcag
     8581 cgatgttggg gtggcctcac ccaacccgga ggtaatggca atgtaatcgc tggggatttc
     8641 tttcaggtaa atggtttgct tctcaacagc acaggtgccc acaagtccct cgccaagcat
     8701 aatacttttt tgaaggaatt tcttccggtt ataggcaaag gccgaaagga gttcgaggtg
     8761 tttttccgat tcgtactcct taaacatgaa aattcctccc tggttagcat caaggtagtt
     8821 taccaggttt tggataacat tatcggcaag gaggttaagg ttattgttgt tttgacgaag
     8881 tatatcgcca aacttggcaa gacctgcgtt aatccatgtg cggatatcat cctccttttt
     8941 acgcttagcc tcttcctgtt gggctcgttt taggctttcg cgcatttgta gtaaagccaa
     9001 tccaagctga tcgtttttgc tgagaggctt aaagtcaaaa tcgagtttgt tttgttcaat
     9061 ttgccgtgca aattcagtag tactcttaag cccgtcgatt aagctgttta gcgactcggc
     9121 taaggttccc agctcgctcc tgtcggtaac cttaacagct ggaggaatct cgccctgtgc
     9181 aagctgtttt agtcgaacgg agaggtgggc cagctgtttt gtgatgggtt taagcaaaag
     9241 ccatacaaca attacagata tcaacgaaga gaggaaaacg atggcaatca tggctctcct
     9301 gttagccttg acctgctcaa gaacttcggt tggacttact gaaatggcaa agtaggcatt
     9361 gtatggtttg tagtacttgt aaacaagtat tcggttctcc gtaatgtcat cggtaatatc
     9421 ctggtagtat atgaagccct cgttggcttt gaggttctta atttcctgaa tggtcttcat
     9481 tgggagtctt gctccctggc gggtaatgtg taaaacgtat tcgccgttcc tggaaatcaa
     9541 tgaggggaaa tcggttttgt tgaatgctgg cttattgaaa tagggcttta gttcaaagat
     9601 atcgtttgag gttaaacctt tcccagtttt ttgctcaact atgttagcaa tgtcaattaa
     9661 cctatcaatg taaacgtaag cctctttaac ggcgttatta atggttctgg tacgggaaaa
     9721 gtaatttagg attaaacctg caaaggcaaa gagcacaacc attaaaggga ttatggcaag
     9781 ttgaaccttt tgtgcaagtt tcatgttagc aaaagccttc atggtaaaat ctattttttt
     9841 atgtaagcct gacctaatcg ttcaaaacgc gtggcccaag ggccaagtat ggtttcgtgc
     9901 atcccaagca gtaatatccc cttggggtaa agattattat gaaagagttc aaaaaccttg
     9961 ttctggaggg agtagttaaa gtaaatgatt acgttacggc aaagtataag gtcgaacttg
    10021 gcaaaaaaga tattgccatc gaccacaagg tcgtgtttgc ggaaaatggg acgctcggtt
    10081 aagaactttt tcatggtgat agtatctctt accttatcga tatcgaagta cttttcgtat
    10141 ggaacgtcgt tatactcctc aaagttgagt gggttttgtt taataacctt atcgaaatta
    10201 tccagatagc cgaggttaaa gcggtatttg tatactcctt gcttagcagc atccagcaca
    10261 tcggagttta ggtcggttgc aaatattttt gccttgtcga gcatttccat ctcgttcagg
    10321 agaatcatca tggagtaaac ctcctgtccg gtggagcaac cggcatgcca aatatttatg
    10381 gttttgttgt tcttgaatcg gggtagaata cgggagcgca aaagatgcca aaccggtgga
    10441 tcgcggaata gctctgttgt attaaccgtt acttccttga ccactttctc cagaaaatcc
    10501 ctatcgagcc ggagcgaggt aagcagggag gttaggtcga ggttataatc ttcaagaatt
    10561 ttttgcaaac ggcgcttaag cgacttctcc gaatactcgg aaaagtcata ctttgaaaca
    10621 cttttaatag tatttacaaa atactgtaaa tcctggtcgg ttacctgcat acgctggctt
    10681 ggcattatct cattaagcag atatcatcaa caataatcag gctaaatata gatttttctt
    10741 aaatacaaac aaaatctaaa tggcaaagat agcattaact ttgattaaca tcacctaatg
    10801 gattataaga ttgcattttc cattgatact attggattgg catgagaaca aatgatagtt
    10861 taacagggtt atgatcggaa tgcctgaact ttaagtcggt ggttgcaata tcggtaatat
    10921 gaatattagg cgaaagcagg aagaaatcga gaataaccgt tctacactgg ttactgaatg
    10981 gcctatccag atatcgatta gtaggctttg atgggtcaaa agcccattgc catccgcctg
    11041 gcataaagct gctttcaatg gaaagaggtt tgaaagcctt ggtttcgtat gcatttccga
    11101 acctgtcggg cggaaaattg ggcggtagtt gattccagtc gcctccaatt atcacataat
    11161 ttcccttttc atactcgctt aatgctacct ggcgaatgaa actcatttct gccatcttga
    11221 ggctatcgtt ttcaaatgct gagttatggg tattgataag cacaaattca cggccattac
    11281 ttaccgggaa acgggtaacc agcaggcagc gacgtaggtt gaaaaccctg ttaggccatg
    11341 gcgataggcc aggcagctga actcttaccg cattcattgg ggtaaagcgg ctaagagtca
    11401 taattcctga atttacattg cccattgggt ttttaagtgg aaccggaaca aatgacacct
    11461 gatagttggt ggcaaagaag gtaaagtagt taaccgcttg gccgataatt tgaagttgat
    11521 tggtgttgta tgatcgacgc gatgccaaat ctacttcctg aagaaaaata aaatcggtgc
    11581 ggttttgtag aatgaatttt ttaatggaat cgaggttttc taatgttcgt gctttagtat
    11641 cgcgggtttg ctttccccca tcataaaaaa agtccatatt gtcgcctaac ccggcataac
    11701 caatgttcca tgtaatcagt tggtacggcg tacttatagc aactgtgtcg gggtttagtg
    11761 aaacttgaag ggtaatttcc ctttcaggat tgtagaaggt tatggtgctg tacaccataa
    11821 ataggattgc aaagataaat gcaaaagcta tagttataaa gccaagacgt agagtgtttt
    11881 taatcatttt tttaataatt gattatcaaa tttaaaccaa aaatgttatt ggttcaaacc
    11941 taattttgac gattttatat tattggctat ctttgacgca tattaattgc attagcatga
    12001 agtttggagt agttgtattc cctggttcca actgcgatca ggatacagta tatgtaatta
    12061 aggagattct tgagcaggaa gtagtcaccc tttggcacaa ggaaaccgat tttgatccca
    12121 atattgatgt gataatactg ccgggcggat tctcctatgg cgattactta cgatcagggg
    12181 ccctggccaa gttttcgcca ataatgaaca gggtaattga ttttgccaat aatggggggt
    12241 acgtgtttgg tatttgcaat ggtttccaaa ttctttgcga gagtggactt ttaccgggag
    12301 cattactgca caacaccagc caaaagttca tatgcaagaa tgttcacctg gtacctgaca
    12361 gcaagaacac tgctcttaca aagtacttac cccgcgacag ggcgcttaag gttcccattg
    12421 cccatggcga gggtcgttac tacgccgata aggatacact tgcccaaatg cgaattaacg
    12481 gtcaaatact tttccgctat tgcgatgaga ggggagtggt ttcggaaagt gccaatccta
    12541 atggctctgt ggagaatata gcaggtgttt gcaacgaccg ccacaatgtt tttggtatga
    12601 tgccccaccc agagcgtgcc tcggacgatg aactgggcaa taccgacggc agacttattt
    12661 tcgagtcaat gataaagtat atcagggagt tgagtaatta aagcatacgt aaatcgataa
    12721 ttggatcgtt gggcggatta cctatcagct caacgatttt ttttgcaacc tgctcggttg
    12781 aggcaagcat tcctttctgc ttgtactggt gaaacctctc ggattgtgga aagtatttgg
    12841 ggtcaacttt gcgaaccgag tcctgcatgg gggtgtcaac tatacctggc gctaatgaaa
    12901 ttaccctaac ctttgagtcg cctaacagtt tttgctcagc gcttagcacc attgaaaaca
    12961 tatcgatagc agccttggaa gcgcaatacc cgctccacga ttccactgca tggcgcgctg
    13021 ctccggaact gatgttcaca acggtaatta gtttagcaga gggtaggtat aacgcaagta
    13081 aacggttagt tagtattgca gggctaacgg tattaaccat gaatgttttc tcaagctgtt
    13141 cattatcgat tttacctata ggttttacat cgcctagtat gccagcatta tttataagta
    13201 caacgtgttt ggcattggga aagaaaggta tttctacctc tcttgctgct ccctgttggc
    13261 tgaaatcaat ttgaatatgt ttatacctat ggtggctaac tacatttcta cgtgagtatc
    13321 ctaccacata ggagctgtca ttgcttagta cttggcgagt aagcgccaac ccaattcccg
    13381 agctagtacc ggtaatatag aaataattca tatcctcctt tttagtatca aactaaacag
    13441 ctttataaaa cgatttgttt taaagctgtt agtatgaaaa gtatttactt gatgaagtgt
    13501 cgacgaaccc agcgggggaa aattatgcta cccaggaaga ggtaaggata gtatgtaacc
    13561 agtctccaca gcaaggccat tgccacagaa acaggaacaa ttaatgctgc atcaacaggg
    13621 ataaattctg aaaggtaacg ggtaaaaaca aactcggcaa agccactacc tccgggagtt
    13681 ggactcacta acatcattat ccacattacc a
//
LOCUS       DAOOWJ010000071        13441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4612_length_13441_cov_8.422232, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000071 DAOOWJ010000000
VERSION     DAOOWJ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 13441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13441
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4612_length_13441_cov_8.422232"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(618..1277)
                     /locus_tag="PLW22_07895"
     CDS             complement(618..1277)
                     /locus_tag="PLW22_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU74666.1"
                     /translation="MSISSNTKPDNLIIRNIILDLGGVVLDIDYSLTLKAFENMGVYL
                     GNHTTLTGSNELFNQFDCGLINPDTFRQEFNQMFMVALSPKEFDKAWNALLLSWDTER
                     LKLIERLKKNYRVYLLSNTNVIHFNHYNRLLVEETGKELKDYFHKAYLSFEMGIRKPQ
                     VQIFQRVLSENGLIPSETLFVDDTHEHIDAAKGLGIRTIHLKGGNMAKPLAEALKGLV
                     G"
     gene            1361..1434
                     /locus_tag="PLW22_07900"
     tRNA            1361..1434
                     /locus_tag="PLW22_07900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1395..1397,aa:Ile,seq:cat)
     gene            1588..2550
                     /locus_tag="PLW22_07905"
     CDS             1588..2550
                     /locus_tag="PLW22_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU74667.1"
                     /translation="MKNILVVGAGGQIGSELVPHLQKIYGTNNVVAADINTSCAHHFQ
                     NGPFEVLDALDAQKFAELVRKYRVDTIFNLVALLSATGEKNPQLAWKINMGALMNSLE
                     IARENHCAVFTPSSIGAFGPTSPKDNTPQDTVMRPTTVYGICKVTGEMLSDYYFLKYG
                     VDTRSVRFPGIISNVTLPGGGTTDYAVEIYYGAIQQKHYVCPLPQGTYLDMMYMPDAL
                     DACVQLMEADPSKLKHRNSFNVTAMSFEPSQIYATIKKRIPDFAMEYNIDPVKKAIAD
                     SWPNYMDDSCAREEWGWNPKWNLETMTDDMLKVIGEKYKAGLLK"
     gene            complement(2594..3298)
                     /locus_tag="PLW22_07910"
     CDS             complement(2594..3298)
                     /locus_tag="PLW22_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74668.1"
                     /translation="MVLKSIILSLLLLLPLWVLPQIIIEKPFAAELSHPELSIEQIAI
                     YPDSTVVSLTVVNNADSNGWFCADKNIYIENLNTRQRYYRIGSHGIPTCPTTHNFSRK
                     GETLSFELIFPPIPKNLNSINLIEDCNKACFFFKGIILSNKLNSDIRLFNLGMEYYTK
                     NDIAQAVECFSKVVEEIPANPTHVYGYAYYHLVMIFYTKGDKLTASFWLNQLKTSALP
                     NKDYFVRMVKRDTGME"
     gene            complement(3362..4981)
                     /locus_tag="PLW22_07915"
     CDS             complement(3362..4981)
                     /locus_tag="PLW22_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HOU74669.1"
                     /translation="MRKQLLTIIATGLTTLVMAGGIVTNTNQSATYVRMLARDASTSI
                     DAVYFNPAGLVKMENGFHLYLSNQTIFQKKTIENKFPLLNESKYVGDVAAPLFPDFYA
                     VYKKDKLALGFGIQPNAGGGSAKYNTGLPSFEIPVSVIPASLTANGITTTKYSADINF
                     DGSSVFWGAQINASYAINDMISLSLGARAILAKNTYNGFLKNILINPNQPAFGANYNG
                     ANMVPATQFFTDAANTLNAWSTGATSYYNGLEPIVNGGGGSTLLVNGTSVGLTSDQIT
                     QISGLLVAAGVPQSQIGSIDIQTAQATLGAAAPVFAGKSAAMAANATATADKEVDAIQ
                     TGTGYTPIIGANLTFGDKLNIGIKYEFRTKLTLTNDTKVDGTGLFPDGGKLRYDIPAI
                     LSAGFEYKILQSLRFASGFHYYFDKDAVIESAPGVKKDIEGNLYEIAAGLEYDITDKI
                     LVSAGYLYAKTGVGKGFQTDLTHSLTSNTVGFGGMFKVNDNLSLELAGLFTMYNSDSK
                     EIDYSAYGVPSAIETYNRTNVLFSIGAAYKF"
     gene            complement(5097..5399)
                     /locus_tag="PLW22_07920"
     CDS             complement(5097..5399)
                     /locus_tag="PLW22_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74670.1"
                     /translation="MIVENISIIIYISTKYSPYSMRRAKIYLAIVAYFTLVKRFLSKP
                     LYRFVMRIKQKLGIKSVTRCSYDGRVLPAGFEFFFNGGSHLNLGLEQGFLNQYLRN"
     gene            5482..6630
                     /locus_tag="PLW22_07925"
     CDS             5482..6630
                     /locus_tag="PLW22_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOU74671.1"
                     /translation="MYKKLILPFLLMLCQAVNAQTDSFQFTVKYEIPTSPIKDQQRTG
                     TCWSFATTSFVETELIRMGQPVFDISEMYFVRYAYEGKAQNYVRFQGANNFGQGGQAH
                     DVMNVIRKYGMVTEKEYPGLNYGSEVHAHAELEAVLKAFLDAVVKNPNKKLSTAWFPA
                     YRGILDAYLGAVPDKGNSPLSKVPFNPDDYIEFTSFNHHPFYKRVSLEIPDNWSHDLY
                     YNLPLDEMMQVITYALGNGYSVCWDGDVSEKSFAYRKGVALLPVSKFEELSGSDKERW
                     TGITEKDFTARMANFDAPVPEIKVTQENRQANFDNLTSTDDHLMHLTGMATDQNGTVY
                     FLTKNSWGTEGVYDGYLYMSESYVRMKTIAVMVHKNAIPAEIKKKLAL"
     gene            complement(6632..7612)
                     /locus_tag="PLW22_07930"
     CDS             complement(6632..7612)
                     /locus_tag="PLW22_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU74672.1"
                     /translation="MDVEKIFNISSDAEFENLALEVFRFQYKNVDIYRTYVDLLKVDP
                     NKVMEIYQIPFLPIQFFKSQQILANGLSPEIEFTSSGTTGSETSRHYVAKVDIYKQSY
                     TLGFRHFYGSEQQYCILALLPAYLERTGSSLVYMVDGLIRQSGNPHGGFYLNQLNELA
                     AKLQMLDESGQKCLLIGVSFALLDLAEIHSLRLKNTIVMETGGMKGRRREMVRDELHG
                     ILTAAFGVDSIHSEYGMTELLSQAYSTGKGVFSCPPWMRVLIRETNDPLTIAPVGATG
                     GINIIDFANLYSCAFIQTQDLGRLNHDGTFEVLGRMDSAPIRGCNLLVYG"
     gene            complement(7828..8190)
                     /locus_tag="PLW22_07935"
     CDS             complement(7828..8190)
                     /locus_tag="PLW22_07935"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOU74673.1"
                     /translation="MSQTSRNNDNFFERVYQVTRLVPFGRVTTYGAIARYLGATRSAR
                     MVGWALNSSSSQEEWVPAHRVVNRKGLLTGKIHFPGSNTMQQLLEAEGIRVEDNAVVD
                     FDKYFWDPNVELVKGSKK"
     gene            complement(8162..8872)
                     /gene="trmB"
                     /locus_tag="PLW22_07940"
     CDS             complement(8162..8872)
                     /gene="trmB"
                     /locus_tag="PLW22_07940"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOU74674.1"
                     /translation="MGKNKLRRFAENETFENLFQPSFQEVFNTDYRLKGQWQSGFFKN
                     QNPIVLELGCGKGEYTVGLARMFPDKNFIGIDIKGARMWRGAKTALEEGLKNVAFIRT
                     RIEHIKSFFSQDEVDEIWVTFPDPQPRPSKATKRLTSSRFLNYYIQFLKPNAIVHLKT
                     DSQPLHAYTKALVEQNNLKMLVCTSNLYSEVSNDPILGIKTFYEQQFLDQGLPITYIK
                     FQVNHSGPFHEPNIEEQR"
     gene            complement(8887..9363)
                     /locus_tag="PLW22_07945"
     CDS             complement(8887..9363)
                     /locus_tag="PLW22_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOU74675.1"
                     /translation="MTKLKLLVISVITIALFAACSDNYVYNGYVALPQEQWHADSIAS
                     FRIPVSDTMTYYNLYVNVRNTTDYPFQNLYLFIDIAAPNGAIARDTFECYLADDHGKW
                     LGKGRGKYRDNRFTYRQNVRFGAEGEYRITLQQAMRVEQLKGISEIGFRVELGSNK"
     gene            complement(9509..10471)
                     /gene="ricT"
                     /locus_tag="PLW22_07950"
                     /pseudo
     CDS             complement(9509..10471)
                     /gene="ricT"
                     /locus_tag="PLW22_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            complement(10566..11714)
                     /gene="holB"
                     /locus_tag="PLW22_07955"
     CDS             complement(10566..11714)
                     /gene="holB"
                     /locus_tag="PLW22_07955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOU74676.1"
                     /translation="MFFSDIVGQEQVKERLLLQVKENRVPHAMLLAGPPGTGKLAIAV
                     AFAQFLMCTNKNENDSCGVCPACKKVSKLIHPDLHFVYPGKAGKEKSDTADGATEAGT
                     DVLAIWRKMLLENPYMGETDWYDAIDLNNKQGMINVATASEVIRKLSLKSYESDVKVM
                     IIWLAERMNVQASNKLLKLIEEPPAKTFFILVSENTNLILKTILSRTQQVYVPPLTKS
                     EIAVVLTEKHGIAPNRAGDIARISNGSFYEALRLVNQNTGEYFEQYRNLMRLCYAKRY
                     IDLLDWVDDISRLGREEQKEFLSYFLKLTRESLMLNMGLDDITYLMGDEADFGKKFSP
                     FLGPANVNQVTKAFSHAYECIAQNGNPQITFADMVFSLSKHIGPNPKT"
     gene            11896..13158
                     /locus_tag="PLW22_07960"
     CDS             11896..13158
                     /locus_tag="PLW22_07960"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU74677.1"
                     /translation="MISIDSYSFKGKKAIVRVDFNVPLDKKTFEVTDDTRIRGAMPTI
                     KKILNDGGAAILMSHLGRPDGKPQDKYSLKHVIPAIEKNLGKSIKFAPDCVGEEAKKL
                     AAELKAGEVLLLENLRFYEEEEGKPYSLAEDASEEQKKEAKARVKESQKEFVKKLASL
                     ADCYVNDAFGTAHRAHASTALIAAHFPGNSMFGYLIESELKAMDKVLKAPEKPFTAIM
                     GGAKVSDKILIIENLLERVDNLIIGGGMTYTFIKAQGGEIGKSICEDDKLELARELLS
                     KAKAKGVNVYLPVDAVNADAFDANANTNISKIDQTPAGWMGLDIGPETVKLFSDVILK
                     SKTILWNGPMGVFEMDKFAQGTSEIAKAIAEATAKGAFSLVGGGDSVAAVNKNKLADK
                     ISYVSTGGGAMLEYMEGKELPGIKAIRG"
BASE COUNT         3717 a         2845 c         2913 g         3966 t
ORIGIN      
        1 atttggtgtt tagcgtttga aatggagccc aaatgacgca gatgatttag tttattgttg
       61 acagttggtg gttgtttgta aaagagaatt tagcccccta gtcatgctga gctagccgaa
      121 gcatctcatt gtactttaac tcgattataa taagattcct cgactagctc ggaatgacaa
      181 ttcctttaac atccacgctc cctttgtcat gctgagctag ccgaagcatc ttttaacctt
      241 taattcttga tttttttttg gcaatcgctc tttgtcatgc ttttcagttg cgatgcaacg
      301 attaagtgtt actctttggc atatacatta cgtgcatcgc tatacatgtg agagtagcca
      361 tagtattaca aatttttagc ccctacgggg ctggaaacgt tgttcgttga ccgtgaatcg
      421 ttgttcgtga atatcacgcc acacgttctg tgaaacacca tgtaaactct gtgtaatcct
      481 gtgataccaa tttagttgat ggttgttggt tgatagttga tagtttataa tagattacct
      541 aaaatcttga atctgaaatc ttaaatctga aattttaaat ttttatcctt tatcctttaa
      601 cctttaacct tgccatttca gcccactagc cccttaagcg cctcagcaag cggtttagcc
      661 atatttccgc ctttcaggtg gattgtacgt atccctaacc ctttagcagc atcgatgtgc
      721 tcatgggtat cgtcaacaaa aagtgtttct gaaggtatta agccgttctc gcttaacaca
      781 cgctgaaaga tttgaacttg tggtttcctg atgcccattt caaaggagag gtatgccttg
      841 tggaagtaat ccttaagctc tttgcctgtt tcctcaacaa gcagcctgtt gtagtggtta
      901 aaatggataa cgtttgtgtt gcttagcagg tataccctat aattcttttt taatcgttca
      961 attaatttga gcctttcggt atcccaactc agaagcaaag cgttccatgc tttatcgaac
     1021 tccttggggc tgagagcaac cataaacatt tggttgaact cctgcctaaa ggtatcaggg
     1081 ttaatcaaac cgcaatcgaa ctggttgaaa agctcgttac ttcctgtaag tgtagtatga
     1141 ttgcccaggt aaacacccat gttttcaaag gcttttaggg taaggctata atcaatatca
     1201 agtaccactc cacctaaatc aagtattata tttctgataa tcagattgtc aggttttgta
     1261 ttgctgctaa tactcatatt ttttggactc cctatttgaa aattcaaatg caaatatgta
     1321 attttgcaaa ccaaaataaa aacagtaaaa actgtttttt gggcccatag ctcagcaggt
     1381 tagagcatct gactcataat cagagggtcg ctggttcgag cccagctggg cccacaaacc
     1441 gccgcaaggc ggtttttttt tattcactcc atcaaaaaat ctgcttaaaa atataaattt
     1501 aaacgtgggt tttgggtttg aagtgttaac caaatactat acatttgcga aaaatcataa
     1561 gtataatcac cttctaaaaa taaaagcatg aaaaacatat tggttgttgg tgccggtggg
     1621 caaattggtt cagagctggt gccgcatctc caaaagatat atggaaccaa caatgttgtg
     1681 gctgccgata tcaacacaag ctgcgcccat cactttcaga atggtccgtt tgaggtgctc
     1741 gatgcccttg atgcccaaaa atttgctgaa ttggtgcgga agtatagggt tgatactatt
     1801 ttcaacctgg ttgcattact ttctgctact ggcgaaaaaa atccgcagct ggcctggaag
     1861 ataaacatgg gagcactgat gaactcgctt gagattgctc gcgagaacca ttgtgctgtg
     1921 tttaccccca gttccattgg cgcttttggc ccaacatcgc caaaggacaa cacccctcag
     1981 gataccgtta tgcgcccaac tactgtttac ggtatttgta aggttacagg cgaaatgctg
     2041 agcgattact acttcctaaa gtatggagta gatacccgca gtgtacgttt ccccggaatt
     2101 atttcaaatg ttactctccc tggtggtggt actaccgatt acgcagtaga gatttactat
     2161 ggagcaattc agcagaagca ttacgtttgc ccattgcccc aaggtacata tctcgatatg
     2221 atgtacatgc ccgatgccct tgatgcttgc gttcagctca tggaggcaga tccatcaaag
     2281 ctcaagcacc gtaacagctt taacgtaact gcaatgagtt ttgagccctc acaaatttat
     2341 gctaccataa aaaagcgcat ccccgatttt gccatggagt acaatatcga ccctgtaaag
     2401 aaagccatag ctgatagctg gcccaactac atggacgata gttgcgcccg tgaggagtgg
     2461 ggatggaacc ccaagtggaa cttggaaacc atgaccgacg atatgcttaa ggtgattgga
     2521 gaaaagtata aggcaggttt acttaaatag ctttagtata atgatacaaa gggaggtgtt
     2581 gagcctcctt tttttattcc attcccgtat cgcgcttaac cattcgaacg aagtagtcct
     2641 tgttaggaag agctgatgtt tttagttggt tcagccagaa gctagcagtg agcttatcgc
     2701 ccttagtata gaaaatcatt accaaatggt agtaggcgta cccataaaca tgggtaggat
     2761 tagcaggtat ttcctcaact accttgctga aacattcaac tgcttgtgca atatcgtttt
     2821 tggtgtaata ctccatgccc aggttgaata accttatatc ggagtttaac ttattgctta
     2881 gtattatgcc cttaaaaaag aaacaggctt tattgcaatc ctcaataagg ttgatactgt
     2941 tgaggttttt tggaatcgga ggaaaaataa gttcaaacga tagagtttcg cccttacggc
     3001 taaagttatg ggttgtagga catgttggaa taccgtgtga gccaatcctg taataccgtt
     3061 gtcgagtatt caggttttca atgtagatat ttttatcggc acaaaaccag ccattgctat
     3121 cggcgttatt cacaaccgta agacttacca ccgttgaatc cggataaatg gcaatctgtt
     3181 caatggaaag ctctgggtgc gagagttcag ccgcaaaggg tttttcaata atgatttgcg
     3241 gtaaaaccca taaagggagc aaaagtaaaa gtgataggat gattgatttt agaaccatat
     3301 agaaaaaatt taggggttga gaaccaaccc ctaaattagt ataattaatt aacatttaga
     3361 attagaactt ataggcagca cctatcgaga ataaaacatt ggtcctatta taggtttcaa
     3421 tagctgaggg aaccccataa gcagaataat cgatttcttt tgaatcagaa ttatacatgg
     3481 tgaacaatcc tgcaagttca agcgataggt tatcgtttac tttgaacatg ccaccaaagc
     3541 caacggtgtt agatgttaag ctatgggtta aatcggtttg aaagccttta cccacacctg
     3601 tttttgcgta aaggtaacca gctgaaacca gtattttatc agtaatatca tattctaaac
     3661 ctgcagcaat ttcgtaaaga ttaccttcga tatccttttt aacgcctggg gcgctttcaa
     3721 taactgcgtc tttatcaaag tagtaatgaa atccgcttgc aaagcgcaag gattgtaaaa
     3781 ttttatattc aaaacctgcc gataggatag caggaatatc gtaacgaagt tttcctccat
     3841 cggggaatag gccagtacca tcaacctttg tgtcgttggt cagtgttagt tttgttcgga
     3901 attcatattt aataccaatg ttaagtttat cgccaaaagt caagttagca ccaattatag
     3961 gagtataacc tgttccagtt tgaatagcat caacctcctt gtcagcagta gctgtagcat
     4021 ttgctgccat ggctgcagat ttaccagcaa atacaggggc ggcagcgccc agagttgctt
     4081 gagctgtctg aatatcaatt gaaccaattt gcgattgagg aacacctgca gctacaagga
     4141 gtccagaaat ttgagtgatt tgatcagatg ttaatccaac acttgtccca ttcactagta
     4201 atgttgaacc tcctccacca ttaactattg gttctagtcc attgtagtaa ctggttgcac
     4261 cggtagacca cgcattaagg gtgtttgctg catcagtaaa aaattgtgta gcgggaacca
     4321 tattagcgcc attgtaattt gcaccaaaag ctggttggtt aggatttatt agaatgtttt
     4381 taagaaagcc attataggtg tttttggcca aaattgctcg tgcaccaagt gagagtgaaa
     4441 tcatatcgtt tatagcgtaa ctggcattaa tttgtgcacc ccaaaaaaca cttgacccat
     4501 caaagttaat atctgcagag tatttagtag tagtaattcc attagctgtt aggcttgcag
     4561 gtattacaga tactgggatt tcaaagcttg gtaatcctgt attatacttt gcagagccgc
     4621 ctcctgcatt tggttgaatg ccaaaaccta aagctagttt atctttcttg taaactgcat
     4681 aaaaatcagg gaataatggc gctgcaacgt ctccaacata tttggattca tttaaaagcg
     4741 gaaatttgtt ttcgatagtt tttttctgaa aaatggtttg gttactcagg tagagatgaa
     4801 aaccattttc catttttact aaccccgcag ggttaaaata aacagcatcg atactggttg
     4861 atgcatcgcg agcaagcatt cgcacgtagg ttgcactttg gtttgtgttg gttacaatac
     4921 caccggccat taccagcgtg gttaaaccgg ttgcaataat ggttagtagt tgttttctca
     4981 tacttctcat tgtttaggtt agtaatagat tatattctga tttaaacgtt tgtattgaac
     5041 gatggtttga aacggaacga aaaatgttcc cctaatgata ggttaaacga tagaaatcaa
     5101 ttccttaggt actgatttaa aaagccttgc tcaaggccaa ggttgaggtg gctacctcca
     5161 ttaaagaaaa attcaaatcc agccggtaaa accctgccat cgtatgaaca acgggtgacc
     5221 gactttatgc caagtttttg tttgatgcgc ataacaaacc tatagagcgg tttgcttaag
     5281 aaccgcttta ctagagtaaa gtatgcaaca attgccagat aaatttttgc cctgcgcatc
     5341 gagtagggtg aatatttggt gctaatatag ataattatcg atatattttc aactatcaca
     5401 tctttttgta aaataattac atgaaaacgt ttgttggttt taattgcttt tgcataactt
     5461 gcataaataa atccgagttg tatgtataaa aagttaattc tgccattcct gcttatgctc
     5521 tgtcaggcag taaatgcaca aaccgattct tttcagttta cggtaaagta tgagatacct
     5581 acttcgccta taaaggatca gcaacgtact ggcacctgct ggagctttgc aaccacgtcg
     5641 tttgttgaaa ctgagctaat acgaatggga cagcccgtat ttgatatctc cgagatgtat
     5701 tttgtgcgct atgcctatga gggtaaggca cagaactatg ttagattcca gggggccaac
     5761 aattttggtc agggtggaca agcgcacgat gtaatgaatg ttatccgtaa gtatggtatg
     5821 gtaaccgaaa aagaatatcc gggattgaat tacggctccg aggttcacgc ccatgctgag
     5881 cttgaggctg tgcttaaagc atttctggat gctgtggtga aaaacccaaa caaaaaattg
     5941 tcaaccgcct ggttcccagc ataccgtgga attcttgatg cttaccttgg agctgtcccc
     6001 gataaaggaa attcaccttt atcaaaagtc ccctttaatc ctgacgatta cattgagttt
     6061 acctcattca accatcatcc gttctacaaa agggtatcgc ttgaaattcc cgataactgg
     6121 tcgcacgacc tttactacaa tctaccgctc gatgagatga tgcaggtaat aacctatgcc
     6181 ctggggaatg gctattccgt ttgctgggat ggcgatgtga gcgaaaaaag ttttgcatac
     6241 cgcaagggtg tggctctgtt gcccgtatca aaatttgaag agcttagtgg atccgataag
     6301 gagcgatgga ctggtattac tgaaaaggat tttaccgcac gcatggcaaa ttttgacgca
     6361 cctgtgcctg aaattaaggt tacgcaggaa aaccgccagg caaattttga caacctaacc
     6421 tccaccgacg accacttaat gcacctaacc ggtatggcca ccgatcagaa tggaacggtt
     6481 tactttttaa ccaagaacag ctggggaact gagggggtgt acgatggcta tctatacatg
     6541 tccgaatcgt atgtgcgtat gaaaactata gctgtgatgg ttcacaaaaa tgccattcct
     6601 gcggagataa agaaaaagct agcattgtag cctacccgta aacaagtagg ttgcagcccc
     6661 taatgggggc agaatccata cggccaagca cctcaaatgt tccatcgtga ttgagtcgac
     6721 ctaaatcctg cgtttgaata aaagcgcatg agtataggtt tgcaaaatcg attatgttaa
     6781 ttcctccagt agctcctaca ggcgctatgg taagcggatc gttagtttca cgaattaaaa
     6841 cacgcatcca gggtggacag ctgaaaactc cttttcccgt tgagtaggct tgcgatagta
     6901 gttcggtcat gccgtattcg gaatggatac tatcaacgcc aaatgctgcg gtaagtattc
     6961 catgtaactc atcgcgaacc atttccctac ggcggccttt catgccgcca gtttccataa
     7021 caatagtatt cttaagccta aggctgtgta tctcggcaag gtcaagcaag gcaaatgata
     7081 ctccaattaa caggcacttt tgaccggatt catcgagcat ctgaagcttt gccgctagct
     7141 cgtttagctg gttgaggtaa aaacctccgt gcgggttgcc cgactgcctg ataagcccat
     7201 cgaccatgta aaccaacgat gatccggtac gttccaggta agctggtagt agagctagta
     7261 tgcagtattg ctgctcgcta ccatagaagt ggcgaaaacc cagggtgtag ctttgtttgt
     7321 aaatgtcaac cttagcaaca taatgcctgc tggtttcgga acctgtggtt ccgctgcttg
     7381 taaactctat ttcgggcgaa agcccattgg caagaatttg ctggctctta aagaattgga
     7441 tgggaaggaa tggaatctga tagatttcca taaccttatt ggggtcaact tttagtaaat
     7501 caacataggt tcggtaaata tcaacatttt tatactgaaa cctaaatacc tcaagggcaa
     7561 ggttttcaaa ttcagcatca gagcttatat taaaaatctt ttctacgtcc attgcctata
     7621 aatcgtgaac cgttaattgt taaacagggt ttggtttata tagctagaat gttgacttcc
     7681 ttaatcatgc aaacccgaat ttttagagtt tggtgggtca tcccaaattt caaatttaag
     7741 attccaaaac ccgtcccgag ttagcgagtt caagtgcctg ctctggttta tggagttcaa
     7801 gaacctgacc aaatatgcga ctttattcta ttttttgctt ccttttacta actcaacgtt
     7861 aggatcccag aagtacttat cgaaatcaac cacagcatta tcttcaaccc ttatgccttc
     7921 cgcttcaagc agttgctgca tggtattgct accggggaag tggatttttc cggttagcaa
     7981 accttttcgg tttaccaccc tgtgcgcagg tacccactcc tcctggctcg atgatgagtt
     8041 tagcgcccaa ccaaccattc gggctgaacg ggttgctccc aggtatcggg caatggctcc
     8101 ataggtggtt acacgaccaa agggaaccaa ccgggttacc tggtaaaccc gttcaaagaa
     8161 attatcgttg ttcctcgatg tttggctcat ggaaagggcc tgaatggttt acttgaaatt
     8221 tgatatatgt aataggcaaa ccttggtcaa ggaattgctg ctcgtaaaag gttttaatgc
     8281 ctagaatggg atcgttgctt acctcggagt aaagattgct ggtgcaaacc agcattttaa
     8341 ggttattctg ctccaccaac gccttggtgt aggcatgtaa gggttgacta tcggttttaa
     8401 ggtgaactat ggcgtttggt tttaggaatt gtatgtagta attcaaaaat cggctcgatg
     8461 ttaaccgctt ggttgccttc gatgggcgtg gctggggatc ggggaaggtc acccatattt
     8521 catcaacctc gtcctgggaa aagaatgact taatatgctc aatgcgggtt cggataaaag
     8581 ccacattctt caagccctcc tcaagggctg tttttgcacc ccgccacatt cgggccccct
     8641 ttatatcgat accaataaaa tttttatcag ggaacattcg ggctaaaccc acggtgtact
     8701 cgccctttcc gcatccaagt tcaaggacaa tagggttttg atttttaaag aaacccgatt
     8761 gccattgtcc ctttaatctg taatcggtgt tgaaaacctc ctgaaacgaa ggttggaaaa
     8821 ggttctcaaa agtttcgttc tcggcaaaac gacgtagctt atttttcccc acagtaaaat
     8881 caaactttat ttattacttc ccagctcaac cctaaatcca atttcggaga taccttttaa
     8941 ctgctcaacc cgcatggctt gctgcagggt aatcctgtac tcaccttcgg caccaaacct
     9001 tacattttgc ctgtaggtaa agcggttatc gcggtattta cctcttcctt tccctaacca
     9061 tttgccatgg tcatcggcaa ggtaacattc aaatgtgtcg cgtgcaattg cgccattggg
     9121 cgcagctata tctataaaca ggtaaaggtt ttggaatggg taatcggtgg tattcctaac
     9181 gttcacgtaa aggttgtagt aggtcattgt atccgatacc ggaattcgga aactggcaat
     9241 gctatcggca tgccattgct cttgcggtaa agcaacgtat ccgttgtata catagttatc
     9301 ggaacatgcg gcaaatagcg caatggttat gaccgaaatg actaagagct taagctttgt
     9361 catcgttttt atgtttgttt tgtcctttgg gtttgcgacg ttttttcagt ttgggtttcg
     9421 attgcttatc gaagcgggta atgctatcat cctccaagcc atcggtaaaa tcgatatggg
     9481 taaccgcagc ttggggggca acattagtat cgagtaggct gggcacctta atgccttttc
     9541 ggtttagctc aattatctca tttacacgtt ccaccggaac ctcaataagg tttaccgatg
     9601 acgatggctc tgagccaaac cacatactac gcttgaaaat atcggtttta aagtgatagt
     9661 aagtggcatc tttggtttcg agctgtatgt ttccttcagg tatgtcctta cgggcatcaa
     9721 catagcttgc aagctcgtag ttcaagcagc acttcaactt accacattgg ccagctaatt
     9781 tttgggggtt aagtgatagc tcctgtattt ttgctgcatt tgtggtaacc gaatcgaatt
     9841 gggcaatcca ggtagagcag caaagctcgc ggccgcatgt gccgatgcct ccaattctac
     9901 cagcttcctg tcgggcacca atctggcgca tctcaacacg aatcttaaat tcctctgcaa
     9961 gaaccttgat aagctcacgg aaatcgacac gttcatcggc aatgtagtag aatatggctt
    10021 tggttttatc gccctgatac tcaacatcgc caatcttcat gtcgagcttt agcctttcgg
    10081 ctatcttgcg ggagcggatc atagtatcgt gttcaagcgc catggcttcg acccacttct
    10141 caatatcggc cttttttgcc ctgcggtata tcttttttag ctccgctgtt ttgggatcaa
    10201 cgcccatgcg cttcatctgg cgctcaacca gccatcctgt tagcgatact acaccaatat
    10261 cgtgaccggg cgatgcctca acggcaacaa tatcgccttt ctgtaggctt atctgattta
    10321 cattgcggta aaacccctta cgggtgttct tgaactgtat ctcaacaata tcgtattcct
    10381 ggagcgtttc cggaaggtcc tgaacccaat cgtaaacacc aagtttggag cagcaattgc
    10441 cgcaggagct atggtcagta acctcgttta tgtaaactgc gttgcccctt ggggtatata
    10501 ttttttccat attcaatatc aagctaaacc ttgcaaagtt aactaattaa acgtaaaacc
    10561 aactactatg ttttagggtt gggtccaata tgcttactca ggctaaacac catatcggca
    10621 aaggtgattt ggggattacc gttttgggca atgcactcgt aggcatggga aaatgctttg
    10681 gtgacctggt taacatttgc tggccccaga aaaggggaga actttttgcc aaaatcggct
    10741 tcgtcgccca tcaggtaggt aatatcgtcg agccccatgt taagcattaa gctttcgcgg
    10801 gtaagcttta ggaagtaact taggaactct ttttgttcct cgcgcccaag gcgcgagata
    10861 tcatcaaccc agtcgagcaa atcaatgtaa cgcttggcgt aacatagcct catcaggttt
    10921 cggtactgct caaaatactc tccggtgttc tggttaacca atctaagagc ctcatagaaa
    10981 ctaccattgg aaattcgtgc aatatcccca gccctattag gtgctatacc atgtttctca
    11041 gtaagaacaa cggctatttc gcttttcgtt aggggaggca catacacctg ttgggtgcgg
    11101 gaaaggatag ttttgaggat gaggtttgtg ttttcgctaa ccagtatgaa aaaggttttt
    11161 gcggggggct cctcaatgag ttttaaaagc ttattggatg cctgcacgtt catacgttca
    11221 gccagccaaa taatcataac cttaacatcg ctctcgtaac tttttaggct aagcttgcga
    11281 attacctctg aggcagtagc cacgttgatc atgccctgct tgttgttaag gtcaatagcg
    11341 tcgtaccagt ccgtttcgcc catgtagggg ttttccagta acattttacg ccaaatggca
    11401 agcacgtcgg taccagcttc cgtagcgcca tcggcagtat cggatttctc cttgcctgcc
    11461 ttgccggggt atacaaaatg caggtcgggg tggataagtt tcgaaacctt tttgcaggcg
    11521 gggcaaaccc cgcacgaatc gttctcgttt ttgttagtac acattaggaa ttgtgcaaag
    11581 gcaactgcta tcgcaagttt tcctgtaccc ggaggtcctg caagcagcat tgcatgtggc
    11641 acacggttct cctttacctg caatagcaac cgctccttaa cctgttcctg cccaactata
    11701 tcgctgaaaa acatatcgtt ccaaattatt ggctaaactc caaagttaac aattttatgg
    11761 tatagtcaac ttatagtttg aaagcctttc ccaaggcttt gttttcaacc gtttgcgtga
    11821 aaaatattga tttagactct gcaattcact tcgaattttg tatttttgct tcaaacccac
    11881 taaaaacaat tcaatatgat aagcattgac agttacagct ttaaggggaa gaaagccata
    11941 gttagggttg attttaatgt gcccctggat aagaaaacct ttgaggttac tgacgatacc
    12001 cgtattcgtg gcgcaatgcc aacaatcaag aagattctga acgacggtgg tgctgccatt
    12061 ctgatgtcgc atcttggccg ccccgatggg aaaccacaag ataagtattc gttaaagcat
    12121 gttattcccg caattgagaa aaacctgggt aagagcatca agtttgctcc cgattgtgtg
    12181 ggcgaggaag ccaaaaagct tgctgctgag ctcaaggccg gagaggtact attgctcgag
    12241 aatcttcgtt tttatgagga ggaagagggc aagccctata gcttagccga agatgctagc
    12301 gaggagcaaa aaaaggaagc caaggcaagg gttaaagaga gccaaaagga gtttgtaaaa
    12361 aaacttgcca gccttgccga ttgctacgtt aacgatgcct ttggtactgc gcaccgtgcc
    12421 catgcttcca ctgcgttaat tgctgctcat ttcccaggca acagcatgtt cggttacctg
    12481 attgagagcg agctaaaggc tatggataag gttctcaaag ctcctgaaaa gccattcact
    12541 gccattatgg gaggtgctaa ggtgtccgat aaaattttga taattgagaa tctgcttgag
    12601 cgcgttgaca acctgattat tggtggtgga atgacctaca catttatcaa ggctcagggt
    12661 ggagaaattg gaaaatccat atgcgaggac gataagcttg agcttgctcg cgagctgctt
    12721 tccaaggcca aggccaaagg ggttaacgtg tacctgcctg ttgacgctgt aaatgccgat
    12781 gcatttgatg caaatgctaa taccaatatc agcaagattg accaaacccc tgcggggtgg
    12841 atggggctcg atataggtcc tgaaaccgtt aagcttttca gcgatgttat cctaaaatcc
    12901 aaaaccatac tctggaatgg ccctatgggt gtttttgaga tggataagtt tgcccagggc
    12961 acctcggaaa ttgctaaggc tattgccgag gctacagcaa aaggggcatt cagccttgtt
    13021 ggcggtggcg attcagtagc cgcagtaaac aagaataagc ttgccgacaa aatatcgtac
    13081 gtttcaaccg gcggtggtgc catgcttgag tatatggaag gtaaggaact cccaggtatt
    13141 aaagccatca gaggctaaca ttactataaa aaccatattg tgccctaaac agaaaaagtt
    13201 tagggcattt ttatctgtat atttcataaa attgatatca atctagttga atttgttgat
    13261 taatgccatc tcggcctcgc tttttgcttt ttacttttta tttttttatt ttttactttt
    13321 cctctaactt cctctaactt cataatattc attctactcc ggcacattcc cacctaaagt
    13381 aacaacatgt ctagtcctga aataagtcta caggttagaa atagatttaa gcgacacaca
    13441 t
//
LOCUS       DAOOWJ010000072        13007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4809_length_13007_cov_7.973981, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000072 DAOOWJ010000000
VERSION     DAOOWJ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13007
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_13007_cov_7.973981"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="PLW22_07965"
     CDS             <1..1747
                     /locus_tag="PLW22_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOU74678.1"
                     /translation="NYPFQKLDVVLIPDFPYSGMEHPGAIFYRDSRILLDENPSVTQQ
                     LNQANLIAHEVSHQWFGNLVTMNWFNDVWLKEVFAGLMADKIVNPQYSKLNHQLSFVL
                     SHYPRAYSVDRTGGANPIRQNLDNLMLAGTLYGDIIYHKAPIALQQLELMLGEDKFRE
                     GLNIYLKKYAYSNAGWNELIEIFDNLSSDDIKSWSKRWIDAPGMPAIYVDIVSDVINI
                     SQTDNWLPMEFEVMIPTTAGYQTEKVRLNGKHTEIPMPKNFTGLKAIIPNSNGLGYGL
                     FVSADRDLTLLQLNELANNDVARASFFINLHELFLNHLVDTDTYFRFLANALSREKDP
                     QIRNYLLGNLETVWWQFLDHTNQIILSQTLQEALQGIFNNPKIPADERKPAFLTYCRT
                     SITPEAVEFIENIWAGRVQIKGISLSENDFIDLSFQLAVRDVHDADSILQVQEERIQN
                     ADRLAKFRFVRRAATPDEALRDDFFHSLLIAQNRRPEPWVAEGLRLFFHPLRANHSIH
                     YLNAALDLLPEIQQTNDIFFPKTWLDAVLYGHSSTEAAKIVTKWIQEHPQLSPNLKGK
                     LMQSADLLFRAAGSK"
     gene            complement(1748..2902)
                     /locus_tag="PLW22_07970"
     CDS             complement(1748..2902)
                     /locus_tag="PLW22_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOU74679.1"
                     /translation="MSSFRFIDLFAGVGGFHIAMSRLGGKCVFASEIDPSAAETYRIN
                     FGIDPLCDIREVDVSSIPQHDVLCAGFPCQAFSKAGKQQGFDDTRGTLFFEIKRILKH
                     HRPKYIVLENVRNLASHDGGRTWKVISENLKELGYIITEKPLIVSPHQVGVPQLRERV
                     FILGVHSSATKLRKLHFGIPPSHRNQTDGAIVLNGKADRRFAISNYEEYVLSAWDEFI
                     QGVGRLKLGFPIWANEFGKDYDLSNLPTWKAGFVQKNRMLYSQNKVFIDKWLKKYNYL
                     SEFVQTHTKFEWQAGEHIDTVWKGIIQFRPSGIRVKRPTEFPALVAMVHIPVVGWEKR
                     RLTPREVANLQSFPENYIINSNAQQAYKQFGNAVNVKVVEFLAKQLFMQA"
     gene            3074..3784
                     /locus_tag="PLW22_07975"
     CDS             3074..3784
                     /locus_tag="PLW22_07975"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOU74680.1"
                     /translation="MDNLIDSIRSDLKSQATEQNRIAGERFFKENVKLYGLKAAQVQT
                     IAKHYFSSQVKMMSKSEMLALSEQLWQSGFMEETFIACYFTERFVKHFSPEDFVTLEE
                     WVKKYINNWASCDTFCNHTIGDMLMLHPQLIEKVKLWAKSNNRWVRRAAAVSFIVPAR
                     KGLFHNHIVDIATMLLTDSDDMVQKGYGWMLKATSQSNQELVFNFVMSNKAKMPRTAL
                     RYAIEKMPLELKAKAMAK"
     gene            3883..5118
                     /locus_tag="PLW22_07980"
     CDS             3883..5118
                     /locus_tag="PLW22_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74681.1"
                     /translation="MKKSLFITAFSVFTAFISVAQEQTQKPKIEVKPYGYVAWETIFD
                     TYKSVDTRDGELYFYPLKANIDANGTDVNKKHQLQMLSLTTRLGLKISGPDVLGAKTS
                     AMVETDFFATATEYTRLLRVRHAIMTLKWQHSELIMGHYWHPIIVNEVIPSTLAFGAG
                     VPFHSLNRSPQIRFTYYPIEKVRLSATALTQGYHKSMGPAEAQRNSGLPELMAQAAFV
                     KNNSFIAGLSAGYKWLTPRLYITSSNTGTHKTVGQYLFSVFTAANLAKTNIKFETVYG
                     ENPTHLLMIGGYGRVTETTADSDYDYANLRTLSTWLDVDHKLGKWGIGVFAGYSKLLG
                     SNKNYSTIASYHRNDDMNYVYRISPRVSYKEENLVLGIEYMLTTAVYGKTFDAQHKVT
                     SSLDPVSNNRITISAKYSF"
     gene            5224..7164
                     /locus_tag="PLW22_07985"
     CDS             5224..7164
                     /locus_tag="PLW22_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOU74682.1"
                     /translation="MVRVKKALRIGLLLLVLIISLLFLSRNLIVKKIFSKTSARIKNR
                     YGLTVTASAVEVKGLNTLQLQNLLIVDTDTLLCSESFRIKLNPFHLVLLKINPKQIDI
                     SRATINIDNLIAQASKHTLQSSNERKNESSSNKRLYHLVKAFFGLSTATFNINNLHFT
                     YSDSAYTGKVTIEKWSYSNNNFTSTLTLNDGDRASRVTVTGTTSKRKNSLTATVTTEQ
                     PEAYIPFTTPFLGVKASFKKIFLDLNATHIEPGRIELNLVSSINSLLLDGKRIAQKPV
                     IIDSCGANMKIRAIPNSFVIDSLSTVSLNGFKTNLGFEYNPLPNRYVRFTLNTGENSW
                     QSLFDALPKGLFTNLWGIKVNGTFSYSLQVGVPLTNPDSLTIAPNLKSKGFYVLSYGN
                     TNLAMLADTFTHRAYIDNVYVRDIKVNPKSASYASLDQISPYLQWAVITSEDGGFYNH
                     RGFSLDGITYAMACNIREKRFARGGSTITQQLVKNVFLNQSKNIGRKAEEILITWITE
                     NSGVVSKERMLEVYLNIIEWGPDVFGIREASQYYFGKKPSSLTLPEALFLAYIIPRPA
                     KFRYLFDTEGKLKPFVTESFMFVANKMLNRGNITQQDYNNLATNPLVTLTGPAKDLLK
                     KESSLENIDSEGNFTEQEKETN"
     gene            7234..7902
                     /locus_tag="PLW22_07990"
     CDS             7234..7902
                     /locus_tag="PLW22_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU74683.1"
                     /translation="MNTQVSKNIEAVAESIQWFGQAAVKIMNCGKVIYIDPYQIKGND
                     KADLILITHSHFDHFSPDDIKKIVATHTWILCPSDVADEAQKFGAAKVIAVQPGFNDE
                     WHGIEIKTVPMYNLTKTDKHPKSKNWVGYLLNLCHVWLYHTGDTERIPEMKSIKTDII
                     LLPLGQTYTMNSVDDAVAAVLDTGASVAIPIHFGMYEGTTADVDKFKELLKDKVEVIV
                     LPKS"
     gene            complement(7907..8605)
                     /locus_tag="PLW22_07995"
     CDS             complement(7907..8605)
                     /locus_tag="PLW22_07995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU74684.1"
                     /translation="MRTFIIPDIHGCSKTLRFLLEVKLSVCKTDRLFFLGDYIDRGPD
                     TPGVVEYLLNLIDEGFNITCLKGNHEQMMLDCIKGDYEMSLWLMNSGFATTHQYESIV
                     TAIETDYGFLPKRHLNFFTSLKPYVVVDNQYLLVHGGFNYELPNPFADEYAMLWNRPT
                     EAPSSFMPGYKIIHGHTPRALAKIVEEFKDTQRRLYDLDAGCVYKGLYIPGTGYLTAL
                     QLENWRLEFVECLD"
     gene            8610..9410
                     /locus_tag="PLW22_08000"
     CDS             8610..9410
                     /locus_tag="PLW22_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU74685.1"
                     /translation="MVYINLTRKINFAKFFVVMASGYIIKKLQGKVNQAIYGYNLIDQ
                     GDKIMVGLSGGKDSYALLDLLVNRRKVLPIGFELHACHVQATDMTYRADIEFMEQYCA
                     QNNVKFHLRQISVEYNPNDRKPACFICSWKRRKMLFTTTREAGCNKLALGHHLDDAIE
                     TLLLNMINHSSISSIPPKLSMFNGEVFLIRPLTTCLDKELENYSQFMGFPSEKELCQF
                     DKDTHRNTVRELIQHMEQINRDARENIYRSMQNIFDEYIVPKPRKKGN"
     gene            complement(9411..10850)
                     /locus_tag="PLW22_08005"
     CDS             complement(9411..10850)
                     /locus_tag="PLW22_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOU74686.1"
                     /translation="MRPHVIIRYIGMVMLFNAAFIFVSFLISLYNKDAGQLPLLFSFL
                     ITLLWGVFPLVFVPKATDLSNKEGYLVVVLSWILSCFIGSLPLLLYGGEFTFVKALFE
                     SVSGYTTTGASILTNVEALPKGILFWRSSMHWVGGIGIVVFVLIILPSLGKAQMTLSK
                     MEISPLAQENFRYRTRKMLNVIMVVYLGLTVTQVILLRMFGMGLFDAVNHAFATVATG
                     GFSTKNNSVAYFNSVPIDIIIIVFMFLSGIHFGLLYSTITLKKNNIFNSPIVKYYFWI
                     TIIGIALVSINLHGKIYPTWGESIRYAAFQVVSLSTTTGFANADSSIWPPFSILIIIF
                     FTIQCACAGSTAGGMKLDRVVIFFQALKSQILKIQHPQAVIPVRIGKSVINDEIVNSV
                     VQFIVFYILVLFVSTLILTGLGYDSLTSLSATAACMGNAGPGFGLVGSMSNYSMLSDT
                     ALLVLSFDMLLGRLEIFGLLLLFMVKSWR"
     gene            complement(10847..>11068)
                     /locus_tag="PLW22_08010"
     CDS             complement(10847..>11068)
                     /locus_tag="PLW22_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74687.1"
                     /translation="IIVITSVIFTIIFNYMISYYLLSPIKRITKGIQNFTKYRRQYDV
                     TIETRDEMFELNESVKDLILTKSIQKKTE"
     assembly_gap    11069..11168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11169..11480)
                     /locus_tag="PLW22_08015"
     CDS             complement(<11169..11480)
                     /locus_tag="PLW22_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74688.1"
                     /translation="MFKIRYKIITGFAILGIMLVISGLISIYELTKLGNQVNRLLMDN
                     YRSIDFSKQMNFSLSLQERAVMLSIQGDKEKANSLFGNAVSTFNDNLLKASNNLTIPG
                     EA"
     gene            11814..12386
                     /locus_tag="PLW22_08020"
     CDS             11814..12386
                     /locus_tag="PLW22_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU74689.1"
                     /translation="MDEKELVQQVVQHNNHEAFSILVERYKKMVITTCMGFLGNFHDA
                     EDIAQDVFVELYESLPEFRNEAKLSTWLYRIAVNKSLNFLRRKKRETFLQNFLGNKSK
                     DNAGKTIDEIIGKQHSDSNLELKEQHKQLRHAMGMLPENQRIALVLSTYHELSYKEIA
                     EVMETSVSSVESLLFRAKNNLRKIMVKTKP"
     gene            12449..12898
                     /locus_tag="PLW22_08025"
     CDS             12449..12898
                     /locus_tag="PLW22_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOU74690.1"
                     /translation="MRCREVNSLMENYLKGKLDGNTHLNVEEHLGACTRCQQNLLIHK
                     SLKELVNEEASGYTDNPFFASRVISRIEARKISSHIDFRTRLIGYAAVAAALAIGLFI
                     GNQLGTLGSKVISTNTNAEMLAEEYIPSGTETIYEYDFEDLQNLNKK"
BASE COUNT         4097 a         2801 c         2471 g         3538 t
ORIGIN      
        1 caattacccc ttccagaagc tcgatgtggt tctgattccc gattttccat acagcggcat
       61 ggagcatccg ggagcaatat tttacaggga tagtcgtatt ttactcgacg aaaatccctc
      121 ggttacccaa cagctaaacc aggcaaacct aattgcgcac gaggtttcgc accagtggtt
      181 tgggaacctg gttaccatga actggttcaa cgatgtttgg cttaaggagg tatttgccgg
      241 acttatggcc gataaaattg ttaaccctca atactctaag ctaaatcatc agctcagctt
      301 tgtgctctca cattacccca gagcctactc ggtggacaga acaggcggcg caaaccctat
      361 ccgccaaaac ctcgacaatc ttatgcttgc cgggacactt tacggcgata tcatctacca
      421 taaagcaccc attgccctcc agcagcttga gctgatgcta ggtgaagata aattcaggga
      481 aggcctaaat atctacctga aaaagtatgc atacagcaat gccggttgga atgaattgat
      541 tgaaattttc gataacctct cgtccgatga tattaaatcg tggagcaaac gttggattga
      601 tgcacctggg atgcctgcca tatatgttga tatagtgagc gatgtaatca atattagcca
      661 aaccgacaac tggctcccca tggagtttga ggttatgata cctaccactg caggatacca
      721 aacagagaaa gttaggctta atggaaagca taccgaaata ccgatgccaa aaaatttcac
      781 ggggttaaaa gcaatcattc ctaacagtaa tggtttgggg tatggacttt tcgtttctgc
      841 tgatcgcgat ctaactcttt tacagctcaa tgaattagca aacaacgatg tggctcgcgc
      901 ttcattcttc atcaaccttc atgagctttt tctaaatcat ttagttgata ccgatacata
      961 cttccgtttc ctggccaatg cccttagccg agaaaaagac cctcaaataa gaaactacct
     1021 actaggcaat ctggaaactg tgtggtggca attccttgac cacacaaacc aaattatact
     1081 ttcgcaaacc ctgcaggaag ccttacaggg aatatttaac aacccaaaaa tacctgccga
     1141 tgagcgcaaa cctgctttcc tgacctattg cagaacgtct atcactcctg aggctgttga
     1201 attcattgaa aatatttggg caggtagagt tcaaatcaaa gggatttcac taagtgaaaa
     1261 cgacttcata gacctttcgt tccagcttgc ggtaagggac gttcacgatg ccgacagcat
     1321 tctgcaggtg caggaggaaa gaattcagaa tgccgatagg ctagccaagt tcaggtttgt
     1381 aaggcgtgct gccacacccg acgaagccct acgcgacgac tttttccaca gcttgctgat
     1441 tgctcaaaac cgtaggcctg agccatgggt tgccgaaggg ctgcgactct tttttcatcc
     1501 ccttcgagcc aaccattcca tccattacct aaacgctgca ctcgacctac tgcccgaaat
     1561 acagcaaacc aacgatatat tctttccaaa aacttggctc gatgcggtgc tttacggtca
     1621 tagctctacc gaagcagcaa aaattgtaac aaagtggatt caggaacacc cacaactatc
     1681 gcccaaccta aaaggcaagc taatgcaatc ggccgacctg cttttcagag ctgcaggtag
     1741 caagtaatta tgcttgcata aacagctgct tggcaagaaa ctcaaccacc tttacgttta
     1801 cagcattgcc aaactgttta taggcttgct gggcgttgct atttatgatg taattttcag
     1861 ggaaactctg aaggttagct acctcacgcg gcgttagccg gcgtttttcc cacccaacaa
     1921 ccgggatatg caccatagct accagtgctg gaaattccgt aggacgctta acccttatac
     1981 ccgaggggcg aaactgtatt attcccttcc aaaccgtatc gatatgttca ccggcttgcc
     2041 actcaaactt ggtgtgggtt tgaacaaact ccgaaaggta attgtacttt ttcaaccatt
     2101 tatcgataaa aactttgttt tggctgtaaa gcatgcgatt cttctgtaca aaaccagcct
     2161 tccatgtggg caaattgctt aaatcatagt cttttccaaa ctcattggcc caaatgggaa
     2221 agccaagctt aagtctgcct acaccttgaa taaattcatc ccatgccgat agcacatact
     2281 cctcgtaatt gctgatagca aaccgtctat cggccttgcc gttcagcact attgccccat
     2341 ctgtttgatt acgatgcgat ggcggtattc caaaatgtag tttccttagt tttgtggctg
     2401 acgagtgaac cccaaggatg aaaaccctct cgcgaagctg gggaacccca acctgatgcg
     2461 gactaacaat gagcggcttt tcagtgatga tatagcctaa ttcctttaaa ttttccgaaa
     2521 tcactttcca agtccttccc ccatcgtgcg atgcaagatt gcgaacattc tcaagcacaa
     2581 tgtatttggg tcgatgatgc ttcagaatac gctttatttc aaaaaatagc gtaccccggg
     2641 tatcgtcaaa tccctgttgc ttacctgctt ttgaaaaggc ttgacaagga aatccggcac
     2701 atagcacatc gtgctgtgga attgagctta catcaacttc acgaatatca cacaaagggt
     2761 caattccaaa attgattctg tatgtttcag cagcgcttgg gtcaatttcc gatgcaaaaa
     2821 cacacttacc tcccaaccta ctcatggcaa tatgaaaacc acctactccg gcaaaaagat
     2881 cgataaaccg aaaactgctc attttaaaaa tttatgccaa aaatagcatt tgtttagcta
     2941 catttatttt acttcttggt tttataaccc tttttatcat atctacacac ttaaggggtt
     3001 ccaaaccaat ttattacttt aatttcaaaa ccattgaatt aagaatatct ttgtaatatc
     3061 aaacataaaa gaaatggata atttaattga tagcatccgt agcgacctaa aaagccaagc
     3121 aaccgagcag aaccgaattg cgggcgaaag attctttaaa gagaatgtaa aactttatgg
     3181 gcttaaagcc gcacaggttc aaaccattgc taaacattat ttcagcagcc aggtaaaaat
     3241 gatgagtaaa agcgagatgc ttgctctgag cgaacagctt tggcaatcgg gattcatgga
     3301 agaaaccttt attgcatgct actttacaga gcgatttgta aaacattttt cgcctgagga
     3361 ctttgtaacc cttgaagagt gggtaaaaaa atacattaac aactgggcgt cgtgcgatac
     3421 cttttgcaac catacaattg gcgatatgct tatgttgcat ccgcaactaa ttgaaaaggt
     3481 aaagctttgg gcaaaatcca acaaccgatg ggttcggaga gcagcggcag ttagctttat
     3541 tgttcccgcc cgtaagggct tatttcacaa tcatattgtt gatattgcta ccatgctcct
     3601 taccgattcc gacgatatgg ttcaaaaggg ttacggatgg atgttaaaag ccacgagcca
     3661 atcaaatcag gaacttgtat tcaatttcgt gatgtcgaat aaagctaaaa tgcctcgaac
     3721 cgctttaaga tatgccattg aaaaaatgcc actggaactt aaagctaaag caatggctaa
     3781 atagctacac cctaacgttt taccttttac attgaaaatt ttctcacctt ttcaactatt
     3841 attaaagtgc attttaccaa atttgcgcaa aaattttctg agatgaaaaa atcactgttt
     3901 attactgcat tttctgtttt tactgcattt atttcagtag cacaggaaca aacccaaaaa
     3961 cctaaaattg aggttaaacc ttacggttac gtagcatggg aaaccatttt tgatacctat
     4021 aagtcagtcg atacacgcga tggcgagctt tacttctatc ccttaaaggc aaatatcgat
     4081 gctaacggaa ccgatgttaa caaaaagcat cagcttcaaa tgctttcgct aacaacgcgt
     4141 ttgggattaa aaatatccgg gcccgatgta cttggcgcca aaactagcgc aatggttgaa
     4201 accgattttt ttgcaactgc aactgaatat acacgcttac ttagggttag gcatgccata
     4261 atgaccctta aatggcagca ttccgagctc ataatgggac actactggca tccgattatt
     4321 gtaaacgaag taattccctc aactcttgcc tttggtgcag gcgttccctt ccactcgctg
     4381 aatcgctccc ctcaaatccg ttttacttac taccccattg aaaaggtaag gttatcagca
     4441 accgcgttaa cccaaggtta ccataaatcg atgggcccag ctgaggcaca acgcaactcc
     4501 ggactacccg aactcatggc acaggctgca ttcgtaaaaa acaattcatt tattgctggt
     4561 ttgtcggctg gttacaaatg gcttactccc cgactctata ttaccagctc taatactggc
     4621 acacataaaa cagttggtca atacctattt agtgtattta cagcagcaaa tctggctaag
     4681 actaatatca agtttgaaac agtttacggc gaaaatccaa cccaccttct tatgattggt
     4741 ggctacggta gggttactga aaccaccgct gatagcgatt acgactacgc aaacctgcgt
     4801 actcttagta catggctaga tgttgaccat aagttgggaa aatggggcat tggggttttt
     4861 gccggatact caaagctact cggttccaat aagaattact caaccattgc cagctaccac
     4921 cgtaacgacg atatgaatta tgtttaccgt atctcgccac gtgtaagcta caaggaagaa
     4981 aacctggtgc taggaattga atacatgtta accacagcgg tttacggtaa aaccttcgac
     5041 gctcagcata aggttacctc atcgctcgac cctgtaagca ataatcgtat tacaattagc
     5101 gcaaagtatt ccttctagat tgatttaata ctaaaacaga ttccttgtat ttaatagtgg
     5161 cttgcgtatt ggcaagccac ttttttatca ctttatggct aactttgcat aaaattaaag
     5221 gcgatggttc gggtaaaaaa ggctttacgc ataggtttat tactcctggt gttaattatt
     5281 tctcttctat ttctttcacg aaatcttatt gtaaaaaaaa tttttagtaa aacctctgca
     5341 agaataaaaa acaggtatgg tttaacagtt actgcaagcg cagttgaagt taaaggctta
     5401 aacacccttc agctacaaaa cctacttatt gttgataccg acactttgct ttgctctgaa
     5461 tcctttagga ttaagcttaa tccgttccat ttggtattac ttaaaatcaa tcccaagcaa
     5521 attgatatat ccagagctac gattaatatt gataatttga ttgctcaagc aagcaagcat
     5581 acacttcaaa gctcaaatga gagaaaaaat gaaagttcga gcaacaaaag gctttaccat
     5641 ttggttaagg ccttttttgg actttcaacc gcaaccttca atattaataa cctacacttt
     5701 acctattccg attctgctta taccggaaaa gtaaccattg aaaaatggag ttatagcaac
     5761 aataacttta cctcaacgct aaccctaaac gatggcgata gggcttcacg ggtaactgtt
     5821 accggaacaa ccagcaagcg taaaaatagc cttaccgcaa cggtcacaac cgagcaacct
     5881 gaagcctata ttccctttac cacacctttt cttggagtta aagcatcgtt caaaaaaatc
     5941 ttccttgatt taaatgctac ccatattgag cccggtagga tagagttaaa tttagtatcg
     6001 agcattaatt cacttttgct ggatggtaag cgtattgcgc aaaagccagt tataattgat
     6061 tcatgcgggg caaacatgaa aatcagggca atacccaata gtttcgttat tgactctctg
     6121 tcaacagtta gccttaatgg cttcaagaca aatctaggat ttgaatataa tccattacca
     6181 aaccgctatg taaggtttac actcaacact ggcgaaaata gctggcaatc gctctttgat
     6241 gccttaccta aagggttatt cacaaaccta tggggtataa aggtgaacgg aactttcagc
     6301 tactcgcttc aggttggtgt tcccctcaca aaccccgata gcctaaccat cgcaccaaac
     6361 ctaaaatcga aaggatttta tgtgctatcg tacggaaata ccaaccttgc catgctcgcc
     6421 gataccttta cccatagggc ttatatcgac aatgtgtatg ttagagatat taaagttaac
     6481 cccaaaagtg caagctacgc ttcacttgac caaattagtc catacctaca atgggctgta
     6541 ataacaagcg aggatggtgg cttttacaac catcgcgggt tcagcctcga tggtataacc
     6601 tatgcaatgg catgcaacat acgcgaaaaa aggtttgctc ggggtggaag tactattacc
     6661 caacagcttg taaaaaatgt atttctaaat cagagtaaga atattggacg taaagccgaa
     6721 gaaattctta taacctggat tacggaaaat agcggtgtag tatcaaagga aaggatgctt
     6781 gaggtttacc tgaatataat tgagtggggg cccgatgtgt tcggtattcg tgaggcaagt
     6841 caatactact ttggcaaaaa gcccagctca cttacgcttc ccgaagcttt attccttgcc
     6901 tacataatcc ccaggccagc taagtttcgt tacctattcg ataccgaagg gaagcttaag
     6961 ccctttgtaa ctgaaagttt catgtttgtg gcaaataaaa tgctaaaccg tggaaacatc
     7021 actcagcaag actacaataa tctggctacc aatcctctgg taacccttac agggccagca
     7081 aaagatttac tgaaaaaaga atcatcattg gaaaatatcg attctgaagg caattttact
     7141 gaacaggaaa aggaaacaaa ttagcatcaa cccctttaca tcgccctaaa aattcattaa
     7201 atttatgggt gtaaaccaaa tcggattgat gctatgaaca cacaggtatc aaagaatatt
     7261 gaagcagttg ctgaaagtat tcaatggttt gggcaagcag cagtaaaaat catgaactgc
     7321 ggaaaggtaa tatacatcga cccataccaa ataaaaggga acgataaagc cgatttaatt
     7381 ctaataactc actcccactt tgaccacttt tcgcccgacg atataaaaaa aattgtagcc
     7441 acacatacgt ggatactctg tccatccgat gttgccgatg aagcccaaaa atttggagca
     7501 gccaaggtta ttgctgttca gcctggcttt aacgatgaat ggcatggcat tgaaattaag
     7561 accgttccca tgtacaacct aactaaaacc gacaagcacc caaaatcaaa aaattgggtg
     7621 ggctacctgc ttaacctttg ccatgtttgg ctttaccaca ccggcgatac tgagcgtatt
     7681 cccgaaatga agagcattaa aactgatata atactgctac ccttgggcca aacctacacc
     7741 atgaacagcg ttgacgatgc ggttgccgcc gtactcgaca ctggcgcaag tgttgctatc
     7801 cctattcatt tcgggatgta tgagggaacc actgccgatg tggataagtt taaggaattg
     7861 cttaaagata aagtcgaagt gattgtgcta ccaaaatcct agcttgttag tcgaggcact
     7921 ccacaaactc cagcctccag ttttcaagct ggagtgctgt taggtatcct gttcctggaa
     7981 tgtaaagtcc cttgtaaaca cagccagcgt ccaaatcata aagccgacgt tgagtatcct
     8041 taaactcttc tactattttt gctaatgccc gtggagtatg gccatgaata attttatagc
     8101 cgggcataaa actgctaggg gcttcggttg gacggttcca caacatggca tactcatcgg
     8161 caaatggatt tggcagctca tagttaaaac ctccatgaac cagtaggtac tgattatcga
     8221 ctactacata aggttttaga gatgtaaaga aattcaaatg ccgtttaggc aaaaaaccat
     8281 aatctgtctc aattgctgtt acaattgact cgtactgatg cgtagtagca aaacctgaat
     8341 tcattaacca gagactcatc tcgtaatcgc ccttaatgca gtcgagcatc atttgctcgt
     8401 gattgccctt tagacaggta atattaaaac cctcatcaat taagtttaag agatattcta
     8461 caacaccagg tgtgtcaggg cctctatcta tataatcacc cagaaagaat agcctatcgg
     8521 ttttacaaac cgaaagtttt acctccaata aaaatcgaag ggtttttgaa cagccatgaa
     8581 tgtcggggat tataaaagtt ctcatggtaa tggtttatat aaatttaaca cgaaaaatta
     8641 actttgccaa gttttttgtg gttatggcaa gcggatacat tataaaaaaa cttcaaggga
     8701 aagttaatca ggccatttac ggttataatc taattgacca gggcgataaa atcatggttg
     8761 gtttatcggg tgggaaggat agttatgctc tgctggactt actggtaaac cgtcggaaag
     8821 ttcttcccat cggttttgaa cttcacgctt gccacgttca ggctaccgat atgacctacc
     8881 gcgccgatat tgaattcatg gagcaatact gtgcccaaaa caacgtgaaa tttcacttgc
     8941 gtcaaatcag tgtagagtac aacccaaacg ataggaaacc tgcatgcttt atttgcagct
     9001 ggaaacgacg taaaatgcta ttcacaacta cccgtgaggc cggttgtaat aagctggcac
     9061 tggggcatca cctggacgat gccattgaaa ccctactcct caacatgatt aaccactctt
     9121 caataagctc tattccccct aagctctcaa tgtttaacgg cgaggttttc ctaatccgac
     9181 ctttaaccac atgcctggat aaggaactgg aaaattatag ccagtttatg ggctttccat
     9241 cggaaaagga actttgccag ttcgataagg atacacaccg taatactgtc cgcgaactaa
     9301 tccaacacat ggaacagata aatcgcgatg cccgcgaaaa catttaccgt tccatgcaaa
     9361 atatcttcga tgagtatatt gttcccaagc cccgtaaaaa ggggaactaa ctatcgccac
     9421 gacttaacca tgaacaggag cagcaaacca aaaatttcca aacggccaag cagcatatcg
     9481 aacgaaagga caagcagagc cgtatcggat agcattgagt aatttgacat tgacccaacc
     9541 aatccaaagc cagggcctgc atttcccata caagcagcag tagctgaaag cgatgttaaa
     9601 ctatcgtatc ctaaaccggt aagaattaga gtgctaacga ataaaaccaa aatatagaaa
     9661 acaataaact gaaccacaga atttacaatc tcatcgttta tcacgctctt ccctatccga
     9721 acaggaataa cagcctgtgg gtgctgaatt tttaggattt gacttttcag cgcctggaaa
     9781 aagattacca ccctatcgag cttcatacct ccagctgttg aaccagcaca agcgcattga
     9841 atggtgaaga atattatgat tagaattgaa aatggtggcc aaattgatga atcggcattg
     9901 gcaaaacctg ttgtggtcga taacgataca acttggaatg ctgcatacct tatgctctca
     9961 ccccatgtag ggtatatttt accatgcagg ttaattgata ccaatgcaat acctatgata
    10021 gtaatccaaa agtaatactt tacaattggg gaattaaaaa tattattctt ttttagggta
    10081 attgtggaat acagtaaccc aaaatgaatt cccgataaga acatgaacac tataatgatg
    10141 atatcaattg gaacactatt gaaataggct acactattgt tttttgttga aaaacctcct
    10201 gttgctacag tggcaaatgc gtggtttacc gcatcgaaca atcccattcc aaacattctg
    10261 agcagtataa cctgggtaac ggttagccct aagtaaacca ccatgattac attcaacatt
    10321 tttcgggttc tgtacctaaa gttttcctgt gccagcggcg aaatctccat ttttgaaagg
    10381 gtcatctggg ctttacctaa cgatggtaga atgattagca caaatacaac tattcctata
    10441 cctcccaccc agtgcatgga tgagcgccag aacagaattc cttttggtag cgcctcaaca
    10501 ttggttagaa ttgatgcacc tgttgtggta taaccggaaa cactctcgaa taaagcctta
    10561 acaaaggtaa actcgccacc ataaagcagt agcggtaagg aaccaataaa gcacgataaa
    10621 atccagctaa gaactacaac caggtaaccc tccttgtttg agaggtcagt agccttaggg
    10681 acaaaaacca aagggaatac tccccaaagc agagttatta gaaagctgaa tagcaagggc
    10741 aattgcccag catccttatt atacaacgat atcaaaaacg atacaaagat aaatgctgca
    10801 ttaaatagca ttaccattcc aatgtatcgg attatcacat gtggtctcat tctgtcttct
    10861 tttgaatgct cttggtaaga attaggtcct taaccgactc gttaagctca aacatctcat
    10921 cgcgggtttc tattgttaca tcgtactgtc ttctatactt tgtaaaattc tgaatccctt
    10981 ttgttattct ttttattggg cttaacaggt agtatgaaat catgtagttg aaaataatgg
    11041 taaaaataac gctggtaata acaattatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnntg cctcgccagg tatggtcagg ttgttcgatg cctttaacag attgtcgttg
    11221 aatgttgaca ccgcattgcc aaaaagggaa tttgcctttt ccttatcacc ctgaatggat
    11281 aacataacag ccctttcctg gaggcttaag ctaaagttca tctgctttga gaaatcgata
    11341 ctccggtagt tgtccataag aagcctattg acctgatttc ccagcttggt aagctcataa
    11401 atggatatta accctgaaat aaccagcatt ataccaagta tggcaaagcc agttattatc
    11461 ttgtaacgaa ttttgaacat ggtatcagtt tattattgtc ctttatacag tatcacatca
    11521 gcaaattctg acaatttaaa aatatatgca aatgtataac taaagctcaa tagctattca
    11581 ccaaatacga actaaatgtt cagttcacac aactattcgg gaaagataaa cacattttgt
    11641 aatatcaaaa tctgaaaatc acagtattta cagcatgatg ttaaaaaaaa gtaaatgcta
    11701 gataacatgt agagttttta gtccagcatc aacatacgat gacatattat catattaacc
    11761 tgcgcaagtt ttttgtaact tgtttgtcta ttcatgaaac agttttccat tccatggacg
    11821 agaaagagtt agttcaacaa gttgttcaac ataataatca tgaagccttt agcattttag
    11881 ttgaaaggta caaaaaaatg gttattacca cctgtatggg ctttttaggt aactttcacg
    11941 atgccgaaga tattgcacaa gatgtgtttg ttgagctata cgagtcgttg cctgaattcc
    12001 gaaacgaagc aaaactctcc acctggcttt accgaatagc agtaaacaaa tcgcttaact
    12061 tcttaaggcg taaaaaaagg gaaacattct tacaaaactt tttaggcaat aaatcaaaag
    12121 ataatgcagg aaaaacaatc gacgaaatta ttggtaaaca acattctgat agtaaccttg
    12181 aattgaagga acagcacaaa cagcttcggc atgcaatggg catgctaccc gaaaatcagc
    12241 gaattgcgtt ggtgcttagt acataccatg aactttcgta caaagagatt gcggaagtaa
    12301 tggagacttc ggtttcgtcg gttgagtcgc ttttattcag ggctaaaaac aacctgcgca
    12361 aaataatggt taaaacaaaa ccctaaaaaa tgaatcgctt aacgcaagtt ttaagatatt
    12421 aagttgtcaa actaaaaaac aaataaaaat gaggtgcaga gaagtaaata gtctaatgga
    12481 gaattacctt aaaggcaaac ttgatggtaa tactcaccta aatgtagagg aacatttagg
    12541 cgcttgcacc aggtgtcagc aaaatctttt aatccataaa tcccttaaag aacttgtaaa
    12601 cgaagaggct tcaggatata ccgataatcc gttcttcgca agcagggtaa ttagcaggat
    12661 tgaggctcgc aaaatttcta gccatattga tttcagaaca cgtttaattg gatatgctgc
    12721 tgtggcagcc gcattggcaa taggattatt cattggaaac caacttggaa cactgggaag
    12781 taaagttatc tcaaccaata ccaatgccga aatgcttgct gaggagtaca ttccatcggg
    12841 aaccgaaacc atatatgagt atgactttga agatctacaa aatcttaaca aaaaatagag
    12901 catgaactac tttaccagaa cacgagtgct tgtagcaata attgtagtac ttggagtaac
    12961 cttactggct acaataggca caatggttta cggctactac aaacaaa
//
LOCUS       DAOOWJ010000073        12867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4857_length_12867_cov_8.375976, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000073 DAOOWJ010000000
VERSION     DAOOWJ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4857_length_12867_cov_8.375976"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /locus_tag="PLW22_08030"
     CDS             <1..1841
                     /locus_tag="PLW22_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU74691.1"
                     /translation="RLEGVDIPNPNHWGATGTTGGPVCMLNSNLLENSDFISGAFSAE
                     YGNAISGAFDLKMRSGNNEKYEFLGQIGFNGFEFGAEGPIAKSNGSSFIVNYRYSTLG
                     VFDKLGMDLGTVGVPYYQDISFKINLPRTKLGNISLFGLGGLSDIEIWDSRRDTLKDK
                     IDFYGGEGFDLTNGSSMGVVGLNHSFMVNQSTYTKLSVAVSAHQFKTVMDSLSTDLLQ
                     KFPYYRNNFIESTISGNLSFNKRFSTKSQIKIGVIAKLLAFNYYDSVFKKNDNAFLIL
                     RNDEGDALLTQPYINWQYRPTNTLTFNTGLHAIYFFLNREWSVEPRIAIKWAISSNSS
                     INLGYGLHSQVVSLSIVLGRVRLSDDSYIQPNKDLGFVRSHHFVAGYDWLLASHTRLK
                     AEVYYQYIFDAAVDAAESNSYSLLNQGASFVLELPDYVKNGGNGQNYGVELTLERFLN
                     KGYYYLLTASLFNSQYQGSDGITHSTAFNHHYITNALFGKEFNLSNSKKSKKTFSFDA
                     KVSYSGGKRATPWSAVYNPTENRYSREWDTSKAYELQLRDYFKTDLTIRYKLNKLGFT
                     QEWAIEVTNLFNNKNIYGDRFNTRTGESQWVYQLGIMVIPQYRITF"
     gene            1873..2532
                     /locus_tag="PLW22_08035"
     CDS             1873..2532
                     /locus_tag="PLW22_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOU74692.1"
                     /translation="MERKAAVFGSTGLVGYNLLRVLDENRNYHEIVAYRRNLNQSNPF
                     QKVRFVEFGANISIEPDTYDVFICLGTTMKKAGSKEAFKAVDLDMVIEIVHKASQAGV
                     KRIVAISSIGANQSSRNFYLRTKGQMEEELKKFDFELVAIVRPSILLGKRNEYRLGES
                     IGIWLFRAFRFIFVGPLRRYRGIDAGDVAKAMVQLALTAKGKVTVESEVLKQIADVYK
                     P"
     gene            2516..3076
                     /locus_tag="PLW22_08040"
     CDS             2516..3076
                     /locus_tag="PLW22_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU74693.1"
                     /translation="MFTNHSDYFYNLVQNRQSDRAYDTRPVGREVILRIIEAARLAPS
                     ACNAQPWHFIVVDEPELKNKLADAAASRVLGINHFTKQAPVHIVIVMEPANLNSNFGS
                     LVKGKTFPLIDIGIAAEHICLAAKAEGLGSCMLGWFDEPAVKKLLDIPKSRRVPLIIT
                     LGYPASDEVRQKRRKDFNEIVSFNKY"
     gene            complement(3073..4164)
                     /locus_tag="PLW22_08045"
     CDS             complement(3073..4164)
                     /locus_tag="PLW22_08045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU74694.1"
                     /translation="MRIQIFSTSYIVTDQRLQRVANSLAAYGYSVKVFCRKHKNLSYE
                     NNKFNVKYINPLFEKGPLFYFFYNLRIFLRILFSRSDIIYSNDLDTLPGCALGSIVRC
                     KKLIYDSHELFTEVPELIGRPITKFLWHIQEKIFVKRAKAVITVSEGVANELKKRYNL
                     KNIEVIRNVPIRTSTEVFKDKQPTIIYQGALNMGRGIELAIEMMKQLPCYKLLIVGKG
                     DIEIKLRRQMLESDLSGRVQFLGQLTPENLRLVTPTAWLGLSLEEDMGLNYRYALPNK
                     LFDYLAAHVPVLVSDLPEMKRVVEEYGIGIVAQSRKPIELANQIADFFEDKEGYENTL
                     KNVKKAAEKLCWQNEEPRLLNLINSLKKG"
     gene            4282..5295
                     /gene="murB"
                     /locus_tag="PLW22_08050"
     CDS             4282..5295
                     /gene="murB"
                     /locus_tag="PLW22_08050"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOU74695.1"
                     /translation="MFVIKKDISLKPYNTFGIDVKASYYARANTVEKILYAINFASYN
                     KLPIFVLGGGSNILFTRNIEGVVINPAIQGVQLQTDDEQYYVFRVGAGVVWDKFVEFA
                     AENNLGGIENLSHIPGLIGASPIQNIGAYGVEVKDTIVKVEAIEVSSRKLIEFNASEC
                     RFGYRDSIFKQELKGKVIITHVWFRLSRNPSFVLNYGNLAEEVEKLGEVTVQNVRKAV
                     INIRKSKLPDPAELGNAGSFFKNPVVDADKYNALKSEHPDLNGFQVSESFYKIPAGWL
                     IEKAGWKGKQVGRCGVHHKQALVLVNHGGATGTEILELSSSIQKSVKELFGIELEREV
                     NVF"
     gene            5340..6026
                     /locus_tag="PLW22_08055"
     CDS             5340..6026
                     /locus_tag="PLW22_08055"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOU74696.1"
                     /translation="MTNRIMDPIGRLMGLRYKSHPWHGVDVGPNAPSIVTTFIEMVTT
                     DTVKYEVDKVSGYLRIDRPQKYSNVLPALYGFIPQTLCDENVAQIARDKTERNDLAGD
                     GDPLDICVLTEKNIAHGNLLAYAIPIGGLRMIDGNQADDKIIAVLKDDAVYGHIRNID
                     ECPEAIITRLRHYFLTYKDLPGTHRDCEITHVYGVDEAHQIINAALDDYRKKFENLDM
                     ILSQYGMMKP"
     gene            complement(6062..8161)
                     /locus_tag="PLW22_08060"
     CDS             complement(6062..8161)
                     /locus_tag="PLW22_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOU74697.1"
                     /translation="MRYRSLRTEMMVNILGITIVIMAVTLTVVAFKSINTAKKTARET
                     SSLIASDVTRSVASYLEKPFETLYNINVTFSSLKLSGVKNREVYKDVLKKTLDLNKNY
                     LALWAMYEPNMLDENDSKYAGKDGYDEEGRFDISFYKANNTIKSEAGEVSMYSEDYYQ
                     IPFTSGNETVLEPYFYTYTGDETGHSYFETSVAIPVKESGKTIGVIGLDIDLLELSEN
                     IKNQKVYQTGYIFIASYDGIIAAHPNKELTGDTLSNILKIEIADYKKIVDSNSFSTVT
                     LGKGSKQSFVFINPIKIGKTLKPWSVVVVVPVNEVYSESRKLIITIVIVGIIAVALLI
                     MIILYQAGNITMPIFKAVRFANNIANGDLTHKLEHNRSDELGTLQSSLLQMQQKLIEM
                     VIEFKSSSGSIADASSHLNSTAQQVSQSAEELASSSEELSSTMEEMVSNIEQNSHHAI
                     EVEKISLELVTDAQKVKDASERSMASIGSIAEKIQIINDIAFQTNLLALNAAVEAARA
                     GEHGKGFAVVAAEVRRLAERSRMAADEINIQSGESVQITAKATELLNQIIPKIQKTTE
                     LIQEIAHASKEQLTGSEQVNNTTQQLSGVTQQNATISEELAASAEELAAQAEKLLEFA
                     ESFKIETAHGVKKDDSKHQTIESEKTTPKLEPKPKTSEKKVIKPLTKKKAEEPKQPHK
                     GIDIKLKPSDDSEYESF"
     gene            complement(8413..9135)
                     /locus_tag="PLW22_08065"
     CDS             complement(8413..9135)
                     /locus_tag="PLW22_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74698.1"
                     /translation="MKRVFYVLIAVLATFNAEVKSQESQSIKTQLKFDFVSRHLWRGM
                     RNNTTPAVQPTIRFDGNMFFGGFWASYSLGSENVQEIDIYTGLKYKNVDFTIIDYYNP
                     IDTAGWKGDFFEFRNSKTRHTLDAIITINPTSHFPLSLTFATMFYGYDKDSLSKKNLY
                     STYLEAEYTFFKNDNTRISFHIGGTPYKSYYASKTAITNTGITVTRNIHFNSNITIPV
                     KGSFILNPYTHQVFLLAAFTIL"
     gene            complement(9235..10644)
                     /locus_tag="PLW22_08070"
     CDS             complement(9235..10644)
                     /locus_tag="PLW22_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU74699.1"
                     /translation="MIKTHVIKEIVERLGFAPTVGQLEAINTLSEFTVQPTGNDCMLL
                     TGYAGTGKTSLIAAYVKALDKFSINYQLLAPTGRAAKVLAEYSGCNAYTIHKIIYRQQ
                     SNTDGFGRFTLNFNKSKNTIFIVDEASMISNQSFDDSTFGSGKLLTDLIEYVRMGKEC
                     KLMLVGDTAQLPPVGLDISPALNPAELESFGLNVRFAQLSEVIRQQFDSGILQNATWL
                     RELVDSSKNVFPKFSTEYADVKRITGGELLEYLSDEYSKNGREGNIVICYSNKRANRY
                     NQGIRAKILFHEEELSVGDYLMVARNNYYWVNDRPNIGFIANGEIVRVKRIGKRTELY
                     GFRFADVTFELLDYNNEEFDAKLILDSLYMDGPSLGSESMKMLYQEVLADYMHLKTKK
                     ARMKQIQNDPYFNAIQAKFAYAVTCHKAQGGQWPTVFVDQGFFNDDMLTREYLRWLYT
                     AFTRATQKVYLVNFDDKFF"
     gene            10732..11331
                     /locus_tag="PLW22_08075"
     CDS             10732..11331
                     /locus_tag="PLW22_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74700.1"
                     /translation="MKTVYQIALAVIIAILGYFLYESIMEPIRFEKEKNRRYNATIER
                     LKSIRTAQDAYRSRFGKYTGSFDTLINFMKEGQIKVVKQIGSEDDSLAVIQKKVFRDT
                     ILIPVRDTILKGYPYDSLRFVPFTGGEQFELAAGELKTQSGVSVKVFECKAPNKVILK
                     NLDRQEIINMDDLAKKLNKYPGLKVGSLTEATNNAGNWE"
     gene            11342..12190
                     /locus_tag="PLW22_08080"
     CDS             11342..12190
                     /locus_tag="PLW22_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOU74701.1"
                     /translation="MQEIDIIDETFDRDRTSTYELSIQVSLNGFSYAVKDTIRNTFIA
                     LFNTGWQGFSIGSVDFRNFIGKLTEAKPWLKGEFKKVIVAFNLPYFTIVPNEFFEPSK
                     SKLLLEQVHSIPDGFELGFTELPSLSLVIIFAAPHEFINEWKSLHGGSLFTHSLLSFI
                     QVPYSYTAKPFLQVDIDQQHLAIILHNNGQLLAANSYLCKSTTDVVYYTNALARSVID
                     NTDNLQLNLTGDGELMAGLPEEIGKYFNSITHQANFHKPVFFSYRLLRYRAEYFRLFN
                     LGVTCE"
     gene            12181..12723
                     /locus_tag="PLW22_08085"
     CDS             12181..12723
                     /locus_tag="PLW22_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOU74702.1"
                     /translation="MRIVSGSLRGRLINPPKNFRARPTTDFAKESLFNIIANHFDFEG
                     LRVLDLFSGTGSISYEFISRGAALVDSVEKDFHHWRFIRNTANQLKLIGLNPIKGDSF
                     SYIKSCNRQYDIIFADPPYDLQGIDTLPQIIFNHPVIVDGGWFILEHSNKYNFTDNQH
                     FLEHRVYGSVNFSIFQKKNF"
BASE COUNT         3630 a         2510 c         2688 g         4039 t
ORIGIN      
        1 ggcgcttaga gggtgttgat attcccaacc caaatcattg gggagcaacc ggaaccacag
       61 gtgggcctgt ttgtatgctt aacagtaacc ttttggaaaa ctccgacttt atttcagggg
      121 cattttcggc agagtatggt aatgccatct cgggtgcatt tgacttgaaa atgcgtagcg
      181 gaaacaacga aaaatatgag tttttgggtc aaataggctt taatggtttt gagtttggag
      241 ctgaggggcc gattgctaaa agtaatggtt cctcgttcat tgttaattac aggtactcaa
      301 ccctaggtgt ttttgataag cttggaatgg atctgggaac agtgggtgtg ccttattacc
      361 aggacatatc ttttaaaata aatttacctc gaactaagct tggaaatatc tccttgtttg
      421 gattaggcgg attaagcgat attgaaattt gggatagccg tagggatacc cttaaagata
      481 aaatcgattt ttatggtggc gaagggtttg accttactaa tggttccagt atgggtgttg
      541 tggggttaaa ccattcattc atggtaaatc agagtactta cacaaaactt tcggttgctg
      601 ttagcgctca ccagtttaaa acggtaatgg actcgctttc aaccgattta ttacaaaaat
      661 ttccatacta ccgcaataac tttattgaga gcacaatatc aggaaacctg tcatttaata
      721 aacggttctc aactaaaagc caaataaaaa taggtgtaat agccaagctg ctagcattta
      781 actattatga tagcgttttt aaaaagaacg ataacgcatt ccttattcta aggaatgatg
      841 agggtgatgc attgttaaca cagccttata ttaattggca gtatcgtcca accaatacgc
      901 ttacgttcaa taccggttta catgccattt actttttcct gaacagggaa tggtctgttg
      961 agcctcgcat tgccattaag tgggcaatat cttccaatag ttcaattaat ctagggtatg
     1021 ggttacatag tcaggttgtg tcgttatcca ttgtgcttgg gcgtgtacga cttagcgacg
     1081 actcttatat ccagccaaac aaggatttgg gctttgttcg tagccaccat tttgttgcgg
     1141 gttacgattg gttactagcc tcgcataccc ggcttaaagc ggaggtttac taccagtata
     1201 tttttgatgc tgctgtagat gcagctgaat ctaattctta ctcgctgtta aaccagggtg
     1261 caagttttgt tctggagtta ccggactacg ttaagaacgg tggcaatgga caaaactatg
     1321 gtgttgagtt aacattggag cgtttcctaa acaagggcta ctattaccta ttaactgcat
     1381 cactttttaa ttcacagtat cagggtagcg atggtattac ccacagcact gcatttaacc
     1441 accactacat tacaaatgct ctatttggta aggagtttaa tctgtccaat agcaaaaagt
     1501 caaagaagac ttttagtttt gatgccaagg tgtcatacag tggtggaaag cgagccactc
     1561 cttggtcagc tgtttacaac cctactgaga acaggtattc cagggaatgg gatacaagta
     1621 aggcttatga attacagctt cgcgattatt ttaaaaccga cttgactatt aggtacaagt
     1681 tgaacaagtt aggatttacc caggaatggg caattgaggt tacaaatttg tttaacaata
     1741 agaatatcta tggcgatagg tttaatacac gcacaggcga atcgcagtgg gtctatcagc
     1801 ttggaataat ggttataccc caatacagaa taacctttta gtggtttgtt tgttactttt
     1861 taaaaaactg ttatggaacg aaaagccgct gtatttggaa gcactggtct agttggttat
     1921 aacctgctcc gtgtgctcga tgagaataga aattatcatg agatagttgc ctaccgaagg
     1981 aatttaaacc aatcaaatcc attccaaaag gtcagatttg ttgagtttgg agcgaatatc
     2041 tcaattgagc ctgatactta tgatgttttc atatgcttgg gaactaccat gaagaaggct
     2101 gggagcaaag aggcatttaa agctgtagat ctcgatatgg ttattgaaat tgttcacaaa
     2161 gcttcccagg caggagttaa acgaattgtt gctatttcat ccataggtgc aaatcaaagt
     2221 tcacgtaact tttacctgcg gacaaaggga caaatggagg aggaactcaa aaagttcgat
     2281 tttgagctgg ttgctattgt tcgtccatca atcctacttg gtaagcggaa tgaatataga
     2341 ctaggtgaga gtatagggat ttggttattt cgagcgttta ggtttatatt tgtaggccca
     2401 ttaaggcgat accgaggcat tgatgcaggc gatgtggcta aagccatggt tcagcttgct
     2461 ttaactgcca agggcaaagt aactgtagag tccgaagttt taaagcaaat agcagatgtt
     2521 tacaaaccat agcgattact tttacaacct tgttcaaaac cggcagagcg atagggctta
     2581 cgatacacgt cctgttggta gggaagtaat tcttcgaatt attgaggcag cccgtttggc
     2641 accatcggca tgtaatgccc aaccctggca ttttattgta gttgatgaac cagaactaaa
     2701 aaataaactt gccgatgctg ctgcaagcag agtattgggc atcaaccact ttaccaaaca
     2761 ggctcccgtt cacatcgtta ttgtgatgga acccgctaat ctaaattcta acttcgggag
     2821 tttagtaaag ggcaaaacat tccctctcat tgatattgga attgctgctg agcatatttg
     2881 tcttgctgcc aaggcagagg gacttggtag ctgcatgctg ggttggtttg atgagcccgc
     2941 tgtgaaaaag ttgctggata ttcctaagtc gcgtagggtt cccctaatta ttacccttgg
     3001 ataccctgca agtgacgaag ttcgccagaa acggcgtaag gactttaacg aaatagtatc
     3061 gttcaacaag tactaaccct tttttaacga gttaatcagg ttaagcaacc tgggttcctc
     3121 gttctgccaa catagttttt ctgccgcttt ctttacattt ttcagagtat tttcatagcc
     3181 ctccttatcc tcaaaaaagt cggcaatctg gttggcaagt tctattggtt tccggctttg
     3241 agcaacaatg ccaattccat attcctcaac tacccttttc atttcaggta aatcacttac
     3301 aagcactggc acatgtgcag ctaaataatc aaatagttta ttgggcaaag cgtacctgta
     3361 gttcagtccc atatcctcct cgagcgatag acctaaccat gcagtagggg taacaagcct
     3421 taaattttct ggtgttagtt gccctaaaaa ctgaaccctg ccagaaagat cactttcaag
     3481 catctgccgg cgcagtttta tttcaatatc tcctttacca acaatgagca gcttgtagca
     3541 aggtaactgt ttcatcattt caatggccag ttcaatacct cttcccatat tcagggcacc
     3601 ttgatatata attgttggtt gtttatcttt gaaaacttcc gtggaagttc gtatgggaac
     3661 attcctgatt acctcaatgt ttttcaggtt gtaccttttc tttagctcat tagctacacc
     3721 ctctgaaact gtaattactg cctttgcacg tttaacaaag attttttcct gaatatgcca
     3781 gaggaatttt gtgataggtc ttccaatgag ctctggcact tcggtaaaaa gttcgtgact
     3841 atcataaatc aactttttac accttactat tgagcctaat gcgcatcccg gcaaggtatc
     3901 aagatcgttt gagtaaataa tgtcagagcg cgaaaaaagt attcgtaaaa aaattcttag
     3961 gttgtaaaag aaataaaaaa gaggcccctt ttcaaagaga gggtttatgt atttgacgtt
     4021 gaatttattg ttttcatagc tcaagttttt atgcttgcgg cagaatacct taactgagta
     4081 tccataagct gctagtgaat tggcaacgcg ttgtaaccgc tggtcggtta ctatatatga
     4141 tgtgctaaat atttggattc gcataagtga agtttactta ataacaaatg aatggctttg
     4201 aaggtttagt attagtaaag ctataaaaat ttacgatttt acataaaaaa gttatatttg
     4261 ttatcgcaaa ctaaaagcca aatgtttgtt attaagaaag atatatcgct taaaccttac
     4321 aacacctttg gaatagatgt aaaggcaagt tactacgcca gggcaaacac cgttgaaaag
     4381 attctatatg ccataaattt tgcttcgtat aataagttgc caatctttgt tctaggagga
     4441 ggtagtaaca ttttatttac aaggaatatt gagggcgtag taattaatcc ggctattcaa
     4501 ggagttcaac tccaaaccga cgacgaacaa tactacgtat ttagggttgg tgcaggtgtt
     4561 gtgtgggata aatttgttga atttgctgca gagaacaatc ttggtggaat tgaaaaccta
     4621 tcccatatac ctgggttaat tggcgcatcg cccatacaga acattggtgc atatggggtt
     4681 gaggtaaagg ataccatagt taaggttgaa gccatcgagg tatcttctcg aaaactcatt
     4741 gagttcaatg cttctgagtg ccgttttgga tatcgcgata gtatttttaa gcaggagcta
     4801 aaagggaagg tgattattac ccacgtatgg ttccggcttt caaggaaccc gagttttgta
     4861 ttgaactatg ggaaccttgc tgaggaagtt gaaaagcttg gtgaggttac agtacaaaat
     4921 gtacgcaaag ccgttatcaa catccggaaa agtaaattgc ccgacccggc tgagttaggt
     4981 aatgccggta gttttttcaa aaatcccgtt gttgatgctg ataaatacaa tgcgcttaaa
     5041 tcggaacatc cggatttaaa tggttttcag gtttcagaga gtttctataa aattcctgct
     5101 ggttggctta ttgaaaaagc cggatggaag ggtaagcaag taggtaggtg cggtgtgcat
     5161 cataagcagg ccttggtgct tgtaaaccat ggaggtgcaa ctggtactga aattttagaa
     5221 ttgtcctctt caatccaaaa atccgtgaag gaacttttcg gtatagagct tgagcgggag
     5281 gtgaacgttt tctaatctcg tttcatttat tacctttgcg ggctaaaatt ttttaagcaa
     5341 tgacaaatag gattatggat cccattggca ggctaatggg attgaggtat aaatcgcacc
     5401 cctggcatgg ggttgacgtt gggcctaatg cccccagcat agttacaact ttcattgaga
     5461 tggttacaac cgatacggtt aagtatgagg ttgataaggt atcgggttac ctccggatcg
     5521 atcgtccgca aaagtactca aatgtgcttc cagcacttta tggttttatt ccacaaaccc
     5581 tctgcgacga gaatgtggca caaattgccc gtgataaaac cgaacgtaac gatttagccg
     5641 gcgatggcga tccccttgac atttgcgtgt taaccgaaaa aaacattgca catgggaatt
     5701 tactagccta cgcaatcccc attgggggat tgaggatgat tgacggtaac caggccgacg
     5761 ataaaataat tgccgtttta aaggatgatg cagtttatgg gcatatccgc aatattgatg
     5821 aatgccctga ggcaatcatt acccgactga ggcattactt cctaacctac aaggatttac
     5881 cgggaactca ccgcgattgc gagattaccc atgtatatgg agttgatgag gcacaccaaa
     5941 tcataaatgc agcccttgac gattaccgga agaagtttga aaatctggat atgattctat
     6001 cgcagtacgg gatgatgaaa ccgtaaagta attgagggct gccatttgca gccctcaatt
     6061 gttagaaact ctcgtattca gaatcatcgc ttggttttaa cttaatatca atccccttgt
     6121 ggggttgctt gggctcctca gctttctttt ttgtaagggg ttttataacc ttcttttcgg
     6181 atgtttttgg tttgggttca agctttgggg ttgttttttc gctttcgata gtttggtgtt
     6241 tagagtcatc ctttttaaca ccatgagcgg tttcaatctt aaacgactca gcaaattcca
     6301 acaatttctc agcctgtgca gcaagttcct ctgcgctagc agctaattcc tctgaaatag
     6361 tggcattttg ttgagtaact ccgctaagct gttgggtggt attgttaacc tgctctgagc
     6421 ccgttagctg ttccttgctg gcgtgggcaa tttcctgaat cagttcggtt gttttctgaa
     6481 tttttggaat aatttgattc agtaactctg tggctttagc tgtaatttga acgctctcac
     6541 ccgattgtat atttatttcg tcggctgcca ttcggctacg ttcggctaac cttcgcactt
     6601 cagcggccac aactgcaaac ccttttccat gttcacctgc acgtgcagcc tcaacagcgg
     6661 cgtttagggc taacaggttt gtttggaatg ctatatcgtt aataatttga attttttcgg
     6721 caattgaacc aattgatgcc atacttcttt cggaggcatc cttcaccttt tgggcatcag
     6781 ttaccagttc aagcgatatc ttttcaacct caatggcatg gtgggagttt tgctcaatat
     6841 ttgaaaccat ttcctccatg gtgctcgaca gttcttccga cgatgaagct aactcctcag
     6901 cactttgcga tacctgctgg gctgtagagt ttaagtgtga gctggcatcg gctatacttc
     6961 cactactgct cttgaattca ataaccattt caattagctt ttgctgcatt tgaaggagcg
     7021 atgattggag tgttccaagc tcatcggatc ggttgtgctc cagcttgtgg gttaaatcgc
     7081 cattggctat attgttggca aatcttacgg ctttaaatat tggcatagta atattgccgg
     7141 cttggtatag tattatcata ataagaagcg caactgcaat tatgccaaca ataacgatcg
     7201 tgattattaa ttttcgactc tccgagtaaa cctcattaac gggcacaacc acaactaccg
     7261 accaaggctt aagggttttc ccaatcttta tagggtttat aaatacaaac gattgcttgc
     7321 ttcccttacc caatgtcacc gtgctaaatg aattgctatc aacaattttt ttgtaatcag
     7381 caatttcaat tttcagaata tttgaaagcg tatcgccagt taattcttta ttaggatgtg
     7441 cggctataat cccatcgtat gatgcaataa atatatagcc tgtttggtaa accttttgat
     7501 tcttgatatt ctcagaaagt tctaaaaggt caatatccaa accaattacc cctattgttt
     7561 ttccggactc ttttacaggg atagccaccg aggtttcaaa gtaactatgg ccagtttcgt
     7621 cgccggtgta tgtataaaag tacggctcaa gtacagtttc attgcctgat gtgaagggta
     7681 tctggtagta atcctcgctg tacattgaaa cctcgcccgc ttcgcttttt atggtgttgt
     7741 ttgctttgta aaatgagata tcaaatctac cctcttcatc ataaccatct ttacctgcat
     7801 attttgagtc attctcatca agcatgtttg gttcatacat tgcccaaagg gcaagataat
     7861 tcttatttaa gtcaagcgtt tttttgagta catccttata aacttctctg ttttttacac
     7921 cgctcaattt aagagagcta aatgtaacat tgatattgta tagcgtttca aagggttttt
     7981 caaggtagct ggcaacactc cgtgtaacat ccgatgctat caacgagctt gtttcccttg
     8041 ccgttttctt tgctgtgttt atacttttga atgccacaac ggttaaggtc acagccatta
     8101 ttacaatggt aatacccagg atgtttacca tcatttctgt ccttaagctc ctgtatctca
     8161 tactcttgat ttttgatatt atgcactgtg cttcctgcaa tatacggttg atgcaaataa
     8221 aagtcaaatt ttatttaatt aaatgttcgt tttaaacatt tgttaattaa ataacaatgc
     8281 ttggtagatg cttttcggga agggtcagtg tgtcgcttaa atctattaac taacctgtag
     8341 acttatttca ggactagaca acctttatcc tttatccttt aacctttatc ctttaacctt
     8401 taacctttat ccttacagta tggtaaacgc agctagtaaa aacacttggt gtgtgtaggg
     8461 gttaagaata aagcttccct taaccggaat ggttatgttt gagttaaagt gaatattcct
     8521 agtaactgtt attccagtat ttgtaattgc agtcttgctg gcgtagtaac ttttataggg
     8581 cgttccacca atatgaaatg aaatacgagt attgtcgttt ttaaagaaag tgtactccgc
     8641 ctcaaggtag gtggagtaaa ggttcttttt gcttaatgag tccttatcat aaccataaaa
     8701 catggttgca aaggttaaac ttagcgggaa atggcttgtt gggttaatgg tgattatagc
     8761 atcgagggtg tggcgggtct tactgttcct gaattcgaaa aagtcaccct tccaaccagc
     8821 agtatcaata gggttgtagt aatcaataat tgtaaaatca acgttcttgt actttaaacc
     8881 ggtgtatata tcaatttcct gaacgttttc ggaacctaag gaatacgatg cccaaaagcc
     8941 accaaaaaac atattgccat cgaatcggat agttggctgt acggcggggg ttgtattatt
     9001 tcgcatgcca cgccataggt ggcggcttac gaaatcgaat tttagttgag ttttaatact
     9061 ttgactttct tgactcttaa cctcagcatt aaaagtcgcc agtacggcaa ttaaaacata
     9121 aaaaacacgc ttcatacctc tttccctaaa attgactgca aagattcaac agcaaaacga
     9181 aagggagtaa ggttatgtta atgttgtaga gctgttttat gaaattttaa ctcgctaaaa
     9241 aaatttatcg tcaaagttta ccaggtaaac tttttgggtg gcccttgtaa aagcagtgta
     9301 gagccagcgc aagtactcgc gagttaacat gtcatcgttg aaaaacccct ggtcaacgaa
     9361 aacggtcggc cattgtccac cctgagcttt atggcaggta accgcatatg cgaattttgc
     9421 ttgaatggcg ttaaagtagg gatcattttg aatttgtttc atgcgagcct ttttagtttt
     9481 aaggtgcatg taatcggcca atacttcctg gtaaagcatt ttcatgcttt cgctccccag
     9541 cgatgggcca tccatgtata gagagtcgag tattagttta gcatcaaact cttcgttatt
     9601 atagtcaagc agctcaaatg taacatcagc aaaacggaaa ccataaagtt cagttctttt
     9661 acctatccgc ttaaccctta ctatttcgcc attggctata aagccaatat tgggcctatc
     9721 gttcacccag tagtagttgt tccgggcaac cattaggtaa tcacccacgc tgagttcctc
     9781 ctcgtggaat agaatttttg ctcgtattcc ctgattatat ctattagctc gtttgttaga
     9841 gtagcaaatt acaatattcc cttcgcgccc gtttttactg tactcatcgg agaggtattc
     9901 caggagttcc ccacctgtaa ttcttttcac atcggcatat tcagtgctga attttgggaa
     9961 tacatttttt gacgagtcaa ccagttccct cagccaagta gcattttgca aaattcctga
    10021 atcgaactgc tgtctaatta cctcggagag ctgtgcaaac ctaacattta aaccaaacga
    10081 ttctagctca gcagggttca atgccgggct aatatcaagg cctaccggag gtagctgtgc
    10141 ggtatcgccc acaagcatca gcttgcactc cttgcccatt ctgacatact caataaggtc
    10201 agtaagtagc ttgcccgatc caaatgtgct atcgtcgaac gactggttcg atatcatcga
    10261 agcctcatct acaataaata tggtgttttt tgatttgtta aagtttaggg taaagcgtcc
    10321 aaaaccatcg gtattggatt gttgcctata aattatttta tggattgtgt aagcattaca
    10381 tccggagtat tcagctaaaa cttttgctgc tcttccggta ggagcaagga gctggtagtt
    10441 tatactaaac ttgtccaaag ccttaacgta tgccgcaatt agcgatgttt taccggttcc
    10501 ggcataacct gtaagcaaca tacaatcatt accggtaggc tgaacagtaa actccgataa
    10561 ggtgttaatt gcttccagtt gaccaactgt aggggcaaaa cccaacctct caactatttc
    10621 cttaataaca tgtgttttaa tcacagcaat gcaatatttt ttgtaaattt aagttagaat
    10681 ttaaccagat gtttttaaat ttgcacgcaa acataaacca aaacacgaaa aatgaaaaca
    10741 gtttaccaaa ttgcacttgc agttatcata gcgatacttg gttatttcct gtatgagagc
    10801 ataatggagc caatccgttt tgaaaaggaa aaaaatcgta ggtacaatgc taccattgag
    10861 cgtttaaaaa gtattagaac tgctcaggat gcttaccgct cgcgttttgg taagtacacc
    10921 ggtagtttcg atacccttat caacttcatg aaggaaggtc agattaaggt tgttaagcag
    10981 attggttcgg aggacgactc ccttgctgtt attcagaaaa aagttttccg cgataccatt
    11041 ctcattccgg ttcgcgatac cattcttaag ggatatcctt acgattcgct acgttttgtg
    11101 ccgtttaccg gtggcgaaca gtttgagctg gcagcaggtg agcttaaaac acaatcgggt
    11161 gtaagcgtta aggtttttga gtgtaaagca ccaaacaagg ttatcctaaa gaaccttgat
    11221 cgtcaggaaa ttattaatat ggacgacctt gctaaaaaac ttaataagta tcctggctta
    11281 aaagttggtt ccttaaccga ggccaccaat aatgccggta actgggagta gtatattggc
    11341 aatgcaagaa attgacatca tagacgaaac ctttgatagg gatcggacat ctacatacga
    11401 actatccatt caggtatccc tgaatggatt ttcgtatgcg gtgaaggata ccatacgcaa
    11461 cactttcata gcactattta atacgggttg gcaaggcttt agtatcggta gtgttgattt
    11521 ccgtaatttt attggtaagc taactgaggc taaaccctgg cttaaggggg agttcaaaaa
    11581 ggttatagtt gcatttaacc taccttactt tacaattgtt cccaatgaat tctttgaacc
    11641 tagtaaatcg aaattactcc ttgagcaggt tcattccata cccgatggct ttgaattggg
    11701 ttttactgaa ttgccaagcc tatcgctagt aattatattc gctgcgcccc atgagtttat
    11761 taacgagtgg aaatcgttac atggtggctc tttatttact cattcattac tgtcatttat
    11821 tcaggttccc tacagttaca ccgcaaaacc ctttttacag gttgatattg accaacagca
    11881 tttagccata attcttcaca acaacggtca gcttcttgcg gccaatagct atttgtgtaa
    11941 atcaacaacc gatgttgttt attacaccaa tgcattggct aggtcagtta ttgataacac
    12001 tgataactta cagcttaacc tgactggtga tggagagtta atggcagggt tgcccgaaga
    12061 gataggtaaa tactttaact ctattaccca tcaggctaat tttcataaac cggtattttt
    12121 ctcataccga cttctacgtt accgtgcaga gtactttagg ctattcaatt tgggtgttac
    12181 atgcgaatag tatccggaag tttacgtgga cggcttatta atcctccaaa gaactttcgt
    12241 gctcgtccta ctaccgattt tgccaaggag agcctcttca acataattgc caatcatttc
    12301 gattttgagg ggttaagggt tcttgatttg ttttcgggca caggtagtat aagctacgaa
    12361 ttcatttcac gcggagccgc cttagtggat tcagttgaaa aggatttcca tcattggcga
    12421 ttcatccgta acactgcaaa ccagctaaaa cttattggat taaatcctat taagggagat
    12481 tcttttagct atattaagtc ttgtaacagg cagtacgata ttatttttgc cgaccccccc
    12541 tacgatttgc agggaattga taccttaccg caaatcatct ttaatcatcc agtaattgtc
    12601 gatggaggat ggtttattct tgaacattcc aataagtata actttacaga taatcagcat
    12661 tttttggaac accgagttta cggaagtgtt aatttcagta tttttcagaa aaaaaatttt
    12721 tagctgacta ttgcaagatt aaaaacaagg tattaccttt gcatcgcttt tcaagggaaa
    12781 agttaaagca aaagattggt ccggtagttc agctggttag aatacatgcc tgtcacgcat
    12841 ggggtcgcgg gttcgagtcc cgtccgg
//
LOCUS       DAOOWJ010000074        12724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_4930_length_12724_cov_7.089115, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000074 DAOOWJ010000000
VERSION     DAOOWJ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4930_length_12724_cov_7.089115"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            648..887
                     /locus_tag="PLW22_08090"
     CDS             648..887
                     /locus_tag="PLW22_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74703.1"
                     /translation="METISNCPKLEKCPIYLKNVFFNPNAGETYRKIYCTAGKEKYTS
                     CKRYLVSEKVGKPVPESVMPNCSLTVDEIIAKYNL"
     gene            900..2798
                     /locus_tag="PLW22_08095"
     CDS             900..2798
                     /locus_tag="PLW22_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOU74704.1"
                     /translation="MNLNNLPIGKRLALGFSLIIGLILVVAIAVFISNRKVRLESNKQ
                     VMLNNLLASNAALSSHLLDFIQSGNQVELDSAKFYFTKFKNQLEILKPVVTKKNYGLI
                     TIIEGNIKVVEDQFAILENSNQFIAQTATVLESSSNTALTIAARAMGGEVSRGVQQFL
                     NIRIMEKNYLISGNDELYRKWLTEIDDNTRLASRLGLSEIVRALEQYKGSFIKHVEAK
                     KVIFDAKSKSLEAVTHTQSLLQKGVILSAGLLNDSAKHGLTVIIFVVLSIVFLSSLIA
                     YRVTRSITEPVNSGVELAHNLSKGNLVIHASESAKRGDELGKLSRALNDMSEKLKEVI
                     STIYISSNHIAAASEQLSTTSQQLSQGASEQASSAEEVSASMEQMVANIQNNTENAKQ
                     AELIAFKSVEGIRKGYDSTTYALNSMKKIAEKVTIIGDIAFQTNILALNAAVEAARAG
                     EHGKGFAVVAAEVRKLAERSRLAAEEINRITHDGVKIADDAGRLLSEIVPDIEKTARL
                     VQEIAAASMEQNAGADQINSAIQQLNQVTQQNAAASEEMASGAEELNSQAQQLIDIVS
                     FFDTGGKETLTTLSNLKNVTKKIAQTSITPKTEFKESKRTKSKPKLSDGIDIKLSNNS
                     DDTNYEKF"
     gene            3055..3780
                     /locus_tag="PLW22_08100"
     CDS             3055..3780
                     /locus_tag="PLW22_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74705.1"
                     /translation="MTAVAKIIETRGVMVKEEKVKSLSERIYSNTMVLENYEPYPGYY
                     GKNVPTDVIPRSIFLILVREYDHMFLARTMQKLSREAKHACMGTYGYIDLEDHRYYCI
                     RIKNLRCFEEIPIIQQKLAEHGIDFRRSKPVNSMALITIEKTFLVERFDEGIYLDLAG
                     DNKYYIEIPQELKWDDFKLITSHVKNNLQNSFFDAALGYFWKVDGPQDVIRIYDLKAN
                     LERISEIRSKYLQEIERFNKTHS"
     gene            complement(3781..4452)
                     /gene="ung"
                     /locus_tag="PLW22_08105"
     CDS             complement(3781..4452)
                     /gene="ung"
                     /locus_tag="PLW22_08105"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOU74706.1"
                     /translation="MKPQIEETWKNVLMDEFKKDYFIKLKEFLVEEKKKYTVYPPGSQ
                     IFAAFNHTPFNSVKVVILGQDPYHGPGQAHGLCFSVPKGTPAPPSLQNIFKEINSDLG
                     LPIPNHGNLEKWAKQGVLLLNATLTVRANQAGSHQNKGWETFTDAAIKALSDQHKGLV
                     FILWGNYAQAKTCIIDTNKHFILTAPHPSPLSASRGFFGCKHFSKTNRLLTSISKEPI
                     DWSLD"
     gene            4628..4933
                     /locus_tag="PLW22_08110"
     CDS             4628..4933
                     /locus_tag="PLW22_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74707.1"
                     /translation="MKKVQVSKNKVKNYLSERLARSIVDADENALITVLRYNAIGGFE
                     YLSDEDLFEFLSTSIPELDFVRLAGADDDNLQLEVKKEYKDEEDAIIIDIQRAIQVI"
     gene            5012..6172
                     /locus_tag="PLW22_08115"
     CDS             5012..6172
                     /locus_tag="PLW22_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU74708.1"
                     /translation="MKNFEFFNPVRILFGEGQIDMITQELLPYQTILLTYGGGSIKRN
                     GVYDKVISALKGKKVLEFSGIEPNPRYETLIKAVKLARENNVDFVLAVGGGSVIDGTK
                     FIVASIPFEGEPWDILTKGIKVEKAVPFGTVLTLPATGSEMNAGAVISREATKEKYAF
                     GSPAVYPKFSVLDPTTTYSLPQNQVANGVVDAFAHVLEQYLTYPSNSPIQDRFAEGIL
                     QTLIEVGPKTLENPTDYDLRANFMWSCTMALNGLISTGVPTDWATHMIGHELTAFFGL
                     DHGVTLAIIYPALLSEMVEFKKEKLLQYGSRVWNLNGKNLTPERAIEETEKFFRSLGI
                     KTRLPEHGISFADTKPIVDRFAARGWKMGENKNITSDIVEKILKRAAELYRQ"
     gene            complement(6220..6765)
                     /locus_tag="PLW22_08120"
     CDS             complement(6220..6765)
                     /locus_tag="PLW22_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU74709.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGIMQDQEVAWMPSYGPEMR
                     GGTANVTVILSDSRISSPILQEFDTAIILNQQSLDKFEKMVKPGGTLIYDPNGITRHP
                     QRTDINIYRVEAAEEAARMKNAKTFNMIVLGAFLKVKPIVKLENVIKGLEKSLPQRHH
                     HLIPMNQEAISRGMEIVKKIN"
     gene            complement(6793..7554)
                     /locus_tag="PLW22_08125"
     CDS             complement(6793..7554)
                     /locus_tag="PLW22_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU74710.1"
                     /translation="MDVKDIIKEENLVYSKTKLLTDNIMHYCPGCTHGVVHKVLAEVI
                     DELGIQDKTIGVAPVGCSVLAYNYIDIDWHEAAHGRAPALATAINRLYPNKYVFTYQG
                     DGDLASIGTAEIIHACNRGENIVVIFINNGIYGMTGGQMAPTSLLDMPTSTTPYGRKA
                     ELNGYPLKITELIAQLPGTCYVTRQSGHTPAAVRKLKKAITKAFENTGKKKGTSFIEV
                     VSTCNSGWKLSPVKANEWMVEHMFPYYPLGDLKDE"
     gene            complement(7560..8645)
                     /locus_tag="PLW22_08130"
     CDS             complement(7560..8645)
                     /locus_tag="PLW22_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOU74711.1"
                     /translation="MAELKLMKGNEAIAEAAIRCGCDGYFGYPITPQSEVLETLMARK
                     PWEETGMVVLQAESEVAAINMVYGGAGCGKKVMTSSSSPGISLKQEGITYIAGAELPC
                     LIVNVVRGGPGLGTIQPAQSDYFQAVKGGGHGDYKLIVLAPASVQEMYDFVDLGFELA
                     FKYRNPAMILSDGIIGQMMEKVQIGPYKPRMTDEEIVAKYGSWATTGKTPNRQRNIIT
                     SLDLDAGRQEQFNLKLQRKYKEIEEKEVRFEKFACDDAEYLLVAYGSSSRICQKVVDM
                     AREKGIKVGLLRPITLFPYPTKAIQAMLGQLKGILAVEMSAGQMVEDVRLAVEGKVKV
                     EHYGRMGGMIPTPNDVLKALEEKIIKG"
     gene            complement(8685..8921)
                     /locus_tag="PLW22_08135"
     CDS             complement(8685..8921)
                     /locus_tag="PLW22_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU74712.1"
                     /translation="MAKVRGAIVVDVEKCKGCGLCVVACPSKVIELAREVNGKGYNYA
                     YMANPEACTGCANCALVCPDTVITVYRVKTEAEA"
     gene            complement(8967..10283)
                     /locus_tag="PLW22_08140"
     CDS             complement(8967..10283)
                     /locus_tag="PLW22_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU74713.1"
                     /translation="MKNTPIPYEVVKRKIQESKLPSVGKASIRELVKLVNEIEKETGI
                     KYVRMEMGVPGLEPSQIGIDAEIEALKRGVASKYPMIDGVPELKKEIARFVKNFINID
                     VDEQGCIPTVGSMQGAMAAFLVVNRCNATKDTVLFIDPGFPVQKQQLRVLGMSYETFD
                     VYNFRGDKLRDKLESYLKKGNISAILYSNPNNPSWICFTEKELQIIGELATTYDTIVI
                     EDLAYFAMDFRKDLSKPGQPPFQPTVANYTQNWLMLISSSKAFSYAGQRIGTMVISNS
                     LYKRRYPDLKRYFTSDEFGYAMLYGAVYSLSSGVCHSTQFGFAAMLKAANDGTFNFVE
                     SVREYGEKAKIMKKIFTENGFTIVYDMDEDKPLADGFYFTISYPGLTGEQLIEELLYY
                     GVSAISLAITGSERTEGLRACVSQVQRNQFSDLEERLKRFNQDHRN"
     gene            complement(10536..>12724)
                     /locus_tag="PLW22_08145"
     CDS             complement(10536..>12724)
                     /locus_tag="PLW22_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU74714.1"
                     /translation="GNDQATSVPNQYKSLINLSLSKVAGKNVRLAFVYENRNNQNKGT
                     QLAIDDILVTNTNIPDIELKAIPYKYSKVPHITFDSLFIDLKANIYNLGTVLNDTLTV
                     SASCDKISNLQSSVEITDTLESGEQRVITLSPKVKIMPTSESYTFNLNASTPPDTLTQ
                     NNTDNTTLEVSSSIFATDRGVKGGISFNPNTQIGNLYEFQRDGFIEGVEIGWAQNTAI
                     PETEYPIDFVVYILEINPASPSEAKTLTQEYFTKEYSANGGSIIYFLPKPAYCHKGFS
                     YYVLVKQTSDTPLGIGYDGNPYGAFWKVDQFSPLNATYMANQSIGNIAVRALVSEPVE
                     NPTVFFNIKNEQKEPAQNVKVRIVELDSTITTNAQGQASIKLNNGQYTYSIDSTGYSS
                     LKKKFTVFSQNLIINDSLVKAYNVKFRIVDTDSVPLPGARINVYPITLTSNENGEDST
                     MLPSGNYSLKVMLSNYMMKYPIDLLVEDKDTLYIVVMEQATTYNLTVNVKNTKGESLP
                     NADVSLMGYGTLKTNNEGNCAFNGLLPGNIMGSVKYYNYIWGSIFVDLTSDSTINVTL
                     IPEHYTATFYVTSKGNPIINAEIIIEGLDTLHTGLNGYAQSTYIPFGNSIPYTVKVKD
                     YYTHTGTFDIWDTDVLVTVNLTPLGVDDNYDDLNPTIYPNPTTDFITVTGINNYSISI
                     FDINGRVVYQNSNPDSKIDIGFLPKGIYIVKLNTSKGFSIHKVLKQ"
BASE COUNT         3680 a         2558 c         2645 g         3841 t
ORIGIN      
        1 tacatgcctg tcacgcatgg ggtcgcgggt tcgagtcccg tccggaccgc aaaaaagcct
       61 cgggttactc cggggctttt ttatttttcg atacacaagt caatgtacct cattttaatc
      121 caaacttttc aattgaattt ttcaattgac gaatctcctc ttaaagtctt actacatcct
      181 tattattatt agaacgtaaa agcttgaaaa tccaaggtaa gatttaactg ttccctttcc
      241 ctctaccttc atctaactcc ttcatattcc ttctattcct attcattcta actcattccc
      301 gcctcaagct tggcgtttca cggctcacgt ttcacgggct caaacttcct ctaacttctt
      361 ctaactccaa catattcctc ctattccgat tcattccatt tattccgttt cattccgttt
      421 cattccgact cattccccta aaaatttacc ctgttgaatt tgtaatttca tggtacatca
      481 gtttaatata taccacaaat caataattag gttcctaact cattttcagc aatatttatg
      541 ttgttattta gataacattt aaattgattt tctaaaatat tttttttgac aaatgatttt
      601 ttttctgatt aagaattgat taaatttagt atatctaaaa aaaaagtatg gaaacaatat
      661 caaactgtcc aaaactggaa aagtgcccca tttacctgaa aaacgttttt ttcaatccga
      721 atgcgggcga aacctaccga aaaatttact gcacagctgg taaagagaag tatacaagct
      781 gtaaacgcta ccttgtgtcg gagaaggttg gcaaaccagt acctgaatcc gttatgccta
      841 attgctcctt aacagttgat gaaattatag ctaagtataa tctttaaatc aaactattta
      901 tgaacttaaa caatttacca attgggaaac gattggcatt gggtttttca ttaattattg
      961 gtttaatatt agttgttgcc attgcagttt tcatttcgaa tcgtaaagta aggttggaga
     1021 gcaacaagca ggttatgctg aataatttat tagctagtaa cgctgcttta agttcacact
     1081 tgctcgattt tattcaatca ggtaatcagg ttgaactcga ctccgccaag ttctacttta
     1141 ccaaatttaa aaaccaactc gaaattctta agcccgttgt tactaaaaaa aactatgggc
     1201 taattacaat tattgaagga aatattaagg tagttgaaga tcaattcgca atacttgaaa
     1261 atagcaatca gtttatagct caaaccgcta cagtgctgga gagctcatcg aatactgctc
     1321 ttaccatagc cgcccgagct atgggaggtg aggtttcacg aggcgtacag cagtttttga
     1381 acattcggat aatggaaaag aattacctga tttcaggtaa tgatgaactg tatagaaaat
     1441 ggctaacgga aattgatgat aataccagac tagcatccag attagggctt agtgagatag
     1501 ttagggcact tgagcagtat aaaggaagtt tcattaaaca tgtcgaggcc aaaaaagtta
     1561 tttttgatgc caaaagtaaa tcccttgaag cggtaaccca tacccaaagt ttgctccaaa
     1621 agggagtgat tttatcggct ggattactga acgattcagc taaacatgga ttaacggtaa
     1681 taatttttgt ggttttgtct attgttttct tgagttcatt aattgcatac agggttaccc
     1741 gaagtattac tgaacctgta aactcaggtg tggagctagc ccataaccta tccaaaggga
     1801 acctggttat tcatgcaagt gagtcagcta aaagaggcga tgagctggga aagctttcac
     1861 gtgcccttaa cgacatgagc gagaagctga aggaggttat atcgacaata tatatctcct
     1921 caaatcacat tgcagctgca agcgaacaac tgagtaccac tagccaacag ctatcgcagg
     1981 gagcatcgga gcaggcaagt tctgctgagg aggtttctgc ctcaatggag caaatggttg
     2041 caaatattca aaataacacc gaaaatgcta agcaagccga gctcattgcc ttcaagagcg
     2101 tagagggcat ccgtaaggga tatgattcta ctacatatgc cctgaatagc atgaaaaaga
     2161 ttgcagaaaa ggttaccatt attggcgata ttgctttcca aaccaacata cttgccctta
     2221 atgctgcagt tgaagcagcc agggcaggtg agcatggcaa gggttttgcc gtggtggctg
     2281 ccgaagttcg taagcttgcc gaacgcagcc gtttggccgc cgaggaaatt aacagaatta
     2341 cccacgatgg cgttaaaatt gccgacgatg ctggtcggtt gctctcagag atagttcccg
     2401 acattgaaaa gaccgcaagg ctagttcagg agattgctgc agcaagtatg gagcagaatg
     2461 ctggagccga tcagataaac tctgccattc agcagcttaa ccaggttaca cagcagaatg
     2521 ctgctgctag cgaggaaatg gcttcaggtg ctgaggaact caacagccag gcccagcaac
     2581 taattgatat tgtatcattt tttgacaccg gtggaaaaga aaccctaaca acgttgagta
     2641 accttaagaa tgttactaaa aagattgctc aaacttcaat tactcctaaa accgaattca
     2701 aggaatccaa aagaacaaag tcaaaaccta agctttccga tggtatcgat ataaagctct
     2761 caaataatag cgacgacacc aattacgaaa agttttagca aaaagaggcg ataagcctct
     2821 ttttttgtct tgggatttta ctggacatga cttactctat tccaaattcg tcaattgaat
     2881 tttacacttg aggaatcgac taacataatc tgatattctg aaacctcgga atgaaagact
     2941 ttgataagcc tggattacct cgatatgcca atagaatcac gttatttaaa tgttttatgg
     3001 tgcatctatt gactaacctc ggtttatttt gtaaattgag aaaaattcaa cactatgaca
     3061 gccgttgcta agataattga aacccgtgga gtaatggtta aggaggaaaa ggttaaatcg
     3121 ctttcggagc gaatatactc caacaccatg gttcttgaaa actatgaacc atacccgggc
     3181 tactacggta agaatgtacc aaccgatgta atacctaggt caatcttttt aatcctagtt
     3241 agggagtacg accatatgtt tttagctcgg actatgcaaa aattatcccg tgaggctaaa
     3301 catgcttgca tgggtacata tggatatatt gacctggaag atcatcgtta ctactgtata
     3361 aggattaaaa acctcaggtg ttttgaggag attccaataa tccaacaaaa gcttgctgag
     3421 catggcatcg attttagacg tagcaagcct gtaaatagta tggcccttat tacaatagaa
     3481 aaaacatttc ttgttgaacg attcgacgag ggtatttatc tcgatttagc tggcgataac
     3541 aagtactaca ttgaaattcc ccaggaactg aaatgggacg actttaagct gattaccagt
     3601 catgttaaga ataacctaca gaatagtttt tttgatgctg ccttagggta tttctggaag
     3661 gttgacggcc cacaggatgt aataagaatt tacgatttaa aggccaacct ggaacgaata
     3721 tcggaaatac gttccaaata cctgcaggaa attgaacggt tcaataaaac ccattcgtaa
     3781 tcaatctaac gaccaatcaa taggctcttt actaatggag gtaaggagcc tatttgtttt
     3841 tgaaaaatgt ttacacccaa aaaaacctct gctagccgaa agtggcgatg ggtgcggagc
     3901 cgttaaaata aaatgcttgt tggtatctat aatacacgtt tttgcctgag catagttccc
     3961 ccagaggata aacactaacc ccttatgttg atcggaaagc gctttgatgg cagcatcggt
     4021 aaaggtttcc cagcccttgt tctgatgtga accagcctga tttgccctga ccgtgagcgt
     4081 tgcattcaaa agcagcacgc cctgttttgc ccatttttcc aagttaccgt gatttggtat
     4141 gggaagtcct aaatcgctgt ttatctcttt aaaaatattc tgaagcgagg gtggggcagg
     4201 agtaccttta ggaaccgaaa agcagagccc gtgggcttgc cctggtccat ggtaaggatc
     4261 ttgacctagg ataaccacct taacgctatt gaatggagta tgattaaatg ctgcaaaaat
     4321 ctgtgaacct ggagggtaaa cagtgtattt tttcttctcc tctaccaaaa actctttaag
     4381 tttgataaag taatcctttt taaactcatc cattaaaaca ttcttccagg tctcctcaat
     4441 ttgaggcttg atttcacctg ttgctatgtt catattcctg aaattaaaaa ttaaaacaaa
     4501 gtaaagtaaa tagtaaccac tttaataaat cacacgtaaa aaaaattaaa tttgtattta
     4561 gaacattggg aaaagttaat tgaatgcacc aatacaaaag tagtttaatc ggcaaaaagt
     4621 caaagccatg aagaaagttc aggtttcaaa aaacaaggtt aagaattacc tctcagaacg
     4681 tctagcacga agtattgtgg atgcagatga gaatgctttg attacagtgc tcagatacaa
     4741 tgcaataggt ggatttgaat acctttccga tgaggatttg tttgagtttt tgagcacatc
     4801 aataccagag cttgattttg ttaggcttgc cggggccgat gatgataact tgcaattgga
     4861 ggtgaaaaaa gagtacaaag atgaggaaga tgctatcatt atcgatatac aacgagctat
     4921 tcaagtaatc taatcaatcc gaacgaattt gtttaaccca tcgtaacatt ttgaacgatg
     4981 ggttgtttat tttaataaaa atgttaagcc catgaagaat tttgagtttt tcaatccggt
     5041 aagaattcta tttggcgaag gccaaattga tatgattaca caggaattgt taccttatca
     5101 aacgattctg ctaacctatg ggggtggaag tattaaacgg aatggcgttt acgataaggt
     5161 tattagcgcc ttaaaaggga aaaaagtatt agaatttagc ggaattgagc ctaatccacg
     5221 gtacgagacc ctcataaaag ctgttaagct tgcccgtgaa aataatgtcg attttgtgct
     5281 ggctgttggc ggcggttcag ttattgatgg aacaaagttt attgtggcat caattccttt
     5341 tgagggtgaa ccctgggata ttcttactaa aggcataaag gttgagaaag ctgtaccctt
     5401 tggaacagtg ttaacactcc ctgcgactgg ttcggagatg aatgctggcg cagttattag
     5461 tagggaggca accaaggaaa agtatgcttt tggtagtcct gctgtatacc caaagttctc
     5521 ggttcttgat cctacaacta catattcgct tcctcagaat caggtagcta atggggttgt
     5581 tgatgcattt gcacatgttc ttgagcagta ccttacatac ccatctaatt cgcccatcca
     5641 ggatcgattt gccgaaggta ttctacaaac gctcattgag gttggtccaa aaaccttgga
     5701 aaatccaacc gattacgacc taagagccaa ctttatgtgg agttgtacca tggctctcaa
     5761 tggattgatt tccactggcg tgcctaccga ttgggctacc cacatgatag gtcatgagct
     5821 taccgcattc tttggacttg atcacggagt tactcttgct ataatttatc cggctttgct
     5881 atccgaaatg gttgaattca aaaaagagaa gctactacag tatggcagta gggtttggaa
     5941 cctaaacgga aaaaatttaa cccctgagcg ggcaattgag gaaaccgaga aattctttag
     6001 gagtttgggc ataaaaacac gtttgccaga gcatggaatc agctttgccg acactaaacc
     6061 aattgttgac cgatttgctg caagaggatg gaagatgggc gaaaataaaa acatcacgtc
     6121 cgatattgtt gaaaaaatac taaaacgggc cgcggagcta tacagacaat aaaaaaaagc
     6181 ctgacgttta ggtcaggctt ttattttatc attcatcttc tagttgattt ttttcacaat
     6241 ctccattccc ctgcttattg cttcctggtt catgggaatt aggtgatggt gacgttgggg
     6301 caacgatttc tcaagccctt ttattacatt ttcaagctta acaatgggtt tcactttaag
     6361 gaaagctccc aaaacaatca tgttgaaggt cttagcgttc ttcattctag cagcttcctc
     6421 ggcagcctca accctatata tgttgatatc ggtacgctgc gggtgacgag taataccgtt
     6481 ggggtcatat atcagcgtgc cacccggttt taccatcttt tcgaacttat cgagcgactg
     6541 ctggttaaga atgattgcgg tatcaaattc ctgaagaatt ggggaactaa tacgtgaatc
     6601 gctaagaatt acagttacgt ttgcagtacc gccacgcatt tcgggaccat atgagggcat
     6661 ccaggctact tcctggtcct gcattatgcc tgagtaagca aggattttac ccattgatag
     6721 aacaccttgc ccaccaaaac cggctatgat gatttcttcg gtcatttttt tagtttttaa
     6781 attgattaac tactactcgt ctttcaaatc gccaagcggg taatatggga acatgtgctc
     6841 aaccatccac tcgtttgctt taacgggcga tagcttccaa ccggagttgc aggtcgatac
     6901 tacctcaatg aacgatgtgc cttttttctt tccggtattc tcaaaggcct tagtaatggc
     6961 ctttttgagt ttacgaacgg cagcgggtgt gtgtcccgac tggcgtgtaa cgtagcatgt
     7021 tccggggagt tgggctatta gctcggtaat ctttaagggg taaccgttaa gctcggcttt
     7081 tcgtccgtat ggagtggttg aggtgggcat gtccaataat gaagttggag ccatttgacc
     7141 tccggtcatc ccgtaaatac cattattgat gaaaataacc acaatatttt cgccacggtt
     7201 gcaggcgtgt atgatttcag cagtaccaat tgaggctagg tcgccatcgc cttggtaggt
     7261 aaaaacatac ttgttggggt ataaacggtt gatagcggta gcaagcgcag gtgcacgacc
     7321 gtgggctgcc tcatgccagt caatatcaat gtagttgtat gcaagcacag agcacccaac
     7381 cggtgcaaca ccaatggtct tgtcctgtat gcccagctca tcaataactt cagccagaac
     7441 tttatgaact acaccatggg tacaacccgg gcagtagtgc ataatattgt cggttaggag
     7501 cttggttttg gagtaaacca aattctcttc cttgattata tcttttacgt ccatcttttt
     7561 tagcctttaa taattttttc ttctaaagcc tttaaaacat catttggagt aggaatcatg
     7621 cctcccatgc ggccatagtg ttcaacctta accttacctt caacagccag acgaacatcc
     7681 tcaaccattt ggccggcact catttctact gcaaggattc ctttgagctg ccccagcata
     7741 gcttgaattg ccttggtagg ataggggaat agggttatgg gacgtagtaa tcctactttt
     7801 attccttttt cgcgggccat atcaacaact ttctggcaaa tacggctcga tgaaccatag
     7861 gcaactagca ggtactctgc atcgtcgcat gcaaattttt caaacctaac ctctttttcc
     7921 tcaatctctt tgtatttgcg ctggagtttt aggttgaact gctcctgacg gcctgcatcg
     7981 aggtcaagcg aggtgatgat attgcgttgg cggttagggg ttttaccggt tgtggcccat
     8041 gaaccatact tggctactat ttcctcgtcg gtcatgcggg gtttataggg gccaatttga
     8101 accttttcca tcatctggcc gatgatacca tcggaaagaa tcatggctgg attacggtac
     8161 ttgaaagcga gttcaaatcc aaggtcaaca aaatcgtaca tctcctgaac cgatgctggt
     8221 gcaagaacaa tcagtttata gtcgccatgt ccgccaccct taactgcctg gaagtagtcg
     8281 ctctgggcag gctgaatggt tcccagcccg ggacctccac gtactacgtt aacaattaag
     8341 caaggtagtt cagcaccagc aatgtaggta ataccttcct gtttcaagct tatcccgggg
     8401 cttgatgatg atgtcatgac ctttttaccg caaccagcac cgccataaac catattaata
     8461 gcggcaactt cgctctcggc ttgtaggaca accataccgg tttcttccca tggcttacga
     8521 gccataaggg tttcaagcac ctcggattgt ggggttatgg ggtagccaaa gtaaccatcg
     8581 caaccacaac gtatggctgc ctcggctatg gcctcattcc ctttcattaa ttttaactct
     8641 gccattttct ttaatgagtt tttgtttata caaaatgtcg gtttttaagc ctcagcttca
     8701 gttttaaccc tgtaaacagt gataaccgta tcgggacaaa ccaatgcgca gtttgcgcag
     8761 cctgtgcagg cttcggggtt ggccatgtag gcgtagttgt atcctttgcc atttacctcc
     8821 cttgccagtt caataacttt tgaagggcag gcaaccacac aaaggccaca tcctttacat
     8881 ttctctacat cgaccacaat tgctcctctt acttttgcca tattggtcaa atttattttt
     8941 gttgttttac tatttggttg aaacttttag ttcctgtgat cctggttaaa tcgttttaaa
     9001 cgctcctcca agtcggaaaa ctggttgcgc tgcacctgcg atacacaggc acgtaagccc
     9061 tcagtcctct cactaccggt tatggctaat gaaatggcgc taactccgta gtatagaagc
     9121 tcttcaatta gctgctctcc ggttaaaccc gggtacgata tggtaaaata aaatccatcg
     9181 gccaagggtt tgtcttcatc catatcgtaa actattgtaa aaccgttttc ggtaaaaatc
     9241 tttttcatta ttttggcctt ttcaccgtac tcacgaaccg attctacaaa gttgaaggtt
     9301 ccgtcgttgg ctgcttttag catggctgca aaaccgaact gtgtagaatg gcaaaccccc
     9361 gacgataggg agtaaacagc tccgtaaagc atggcgtagc caaactcatc ggaagtgaag
     9421 tagcgtttca ggtcagggta acgacgcttg taaagactat tgctaataac catagtgcca
     9481 atgcgctgac ctgcatagga gaatgccttg gagctggaaa taagcattaa ccagttttga
     9541 gtatagttag ccacagtagg ctggaacgga ggctgtcctg gtttggatag atccttgcgg
     9601 aagtccatgg caaagtacgc aaggtcctca ataacaatgg tgtcgtaggt tgtggctagt
     9661 tctccaataa tttgcaattc cttctcggtg aagcatatcc acgagggatt attgggattt
     9721 gagtaaagta ttgccgaaat attgcccttt ttgaggtacg attcaagctt gtcgcggagc
     9781 ttatcgccac ggaagttata tacatcaaaa gtttcgtagc tcattcccag taccctaagt
     9841 tgctgttttt gaacagggaa tccgggatcg atgaatagaa cggtatcttt tgttgcattg
     9901 caacggttta ccaccaggaa agcagccata gcaccctgca tggaacctac tgtaggaata
     9961 catccctgtt catcaacatc aatattgatg aagtttttta caaaccgtgc aatttccttt
    10021 tttagctctg gcacgccgtc aatcataggg tacttggagg caacccctcg cttcaatgct
    10081 tcaatctcag catcaatgcc aatttgtgat ggctccagcc cgggcactcc catttccatt
    10141 cttacgtact tgataccggt ttccttttca atctcattga caagtttaac cagctcacga
    10201 atcgaggcct tacctacact tggtaacttt gattcctgaa tctttcgctt tactacttcg
    10261 taggggatgg gcgtattctt catggtatgt tttataatgt attgacaatt tgcttattac
    10321 agatacatat caacagttca cacttcacga attaagcact acaagtttac aaaatatttc
    10381 ttttagtctt tcagaaaaac gtaaaaaaaa gtgataattt gttatcaatt taaattgata
    10441 attaaaataa tattgataaa aaatgatttt atgcttatag aatgtaacaa aaaaggagtg
    10501 ctttgcactc cttttttaac tacattattt tagaattact gctttagaac tttatgaata
    10561 ctaaaacctt ttgatgtatt aagcttaact atgtatatcc cctttggtaa gaaaccaata
    10621 tcaatcttgc tgtcaggatt actattctgg taaactaccc tcccgtttat atcaaaaata
    10681 gagatggaat aattattaat gccggtaact gtaataaaat cggtggtcgg atttgggtaa
    10741 attgtaggat ttagatcatc ataattgtca tctactccaa gaggcgtcag gttaacagta
    10801 acaagaacgt cggtatccca aatatcaaag gtaccagtgt gggtgtagta gtcttttact
    10861 tttactgtat atggtatgct gttcccgaag ggaatgtatg tggactgagc ataaccattt
    10921 agtccggtat gtaaggtgtc taaaccctca ataataattt cggcattgat aataggattt
    10981 cccttggatg taacgtaaaa agtggcagta tagtgctcag ggattagggt tacgttgata
    11041 gtgctatcgg aggtaagatc cacaaaaatt gatccccaaa tataattgta atacttaaca
    11101 gaacccatta tgtttccggg aagtagtccg ttgaaggcac agttaccctc gttatttgtt
    11161 ttaagggttc catagcccat taaactaaca tcagcattgg gtaagctttc acctttagtg
    11221 ttttttacat ttacagtcag gttgtaggtt gtagcttgct ccattacaac aatgtaaagg
    11281 gtatccttat cttcaaccag taaatcaatc gggtatttca tcatataatt ggaaagcatt
    11341 actttaagtg agtagtttcc agaaggtaac atggtactat cttcgccatt ctcattactt
    11401 gtgagggtaa tggggtaaac attaattcta gctccaggta gtggtacgga gtcagtgtca
    11461 acaatcctaa acttcacatt gtaggctttc acaagcgaat cgttaattat tagattctgc
    11521 gagaatactg tgaatttttt cttgagcgat gaatagcccg tagaatcgat gctataggtg
    11581 tattgaccat tgtttagctt gatggaggct tgcccctgtg cgtttgttgt aattgttgaa
    11641 tcgagctcaa caattcgtac cttaacattt tgggcgggtt ccttttgctc attcttaatg
    11701 ttgaagaaaa cagttggatt ttcaacaggc tcgcttacga gtgctcttac cgctatgttg
    11761 ccaatggatt ggttggccat atatgtggcg ttcagcgggg aaaattgatc aaccttccaa
    11821 aaggctccgt aaggatttcc atcgtatcca ataccaagag gagtatcgga ggtttgcttt
    11881 acaagaacat aatacgagaa ccctttatga caataagctg gcttaggcaa gaagtaaata
    11941 attgaaccgc cattagctga gtattccttt gtgaaatatt cctgggttaa agttttggct
    12001 tcacttggac tagccgggtt aatctcaagt atatacacta caaaatcgat cggatattcg
    12061 gtttcaggga ttgcagtgtt ttgggcccaa ccaatttcaa cgccttcaat aaaaccatca
    12121 cgttgaaact catagaggtt acctatttgg gtgtttggat taaatgatat gccccctttt
    12181 acacccctat cggtagcaaa gattgaacta cttacctcaa gggttgtatt atcggtattg
    12241 ttttgagtta aggtatcggg cggggtagaa gcattaaggt tgaaagtgta actttcagag
    12301 gtgggcataa tcttcacttt agggctaaga gttatcactc tctgctcccc actttcaagg
    12361 gtatcagtaa tttcaaccga tgattgtagg ttggatattt tatcgcacga tgctgaaact
    12421 gtcaatgtat cgtttaaaac tgttcccagg ttataaatat ttgctttaag atcgataaaa
    12481 agggagtcaa aagttatgtg aggtactttt gagtacttgt agggaattgc ttttaattca
    12541 atatcaggaa tgtttgtatt ggttactaga atatcatcga ttgcaagttg tgttccttta
    12601 ttctggttat tccgattctc atatacaaag gctaaccgaa catttttacc cgctactttt
    12661 gaaagtgata ggttgattag cgatttatac tggttgggta ccgatgtggc ctgatcatta
    12721 ccat
//
LOCUS       DAOOWJ010000075        12464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5063_length_12464_cov_6.617858, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000075 DAOOWJ010000000
VERSION     DAOOWJ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12464
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5063_length_12464_cov_6.617858"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLW22_08150"
     CDS             complement(<1..575)
                     /locus_tag="PLW22_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74715.1"
                     /translation="MAKEKIKPADQGIELSIEEKLRFLYKLQQVDSKIDKIKLLRGEL
                     PLEVQDLEDEIVGLTTRIDNYKKEIQDLETQINQKKIEIKDAEALIKKYQEQQKNVRN
                     NREYDSLTKEIEYQTLEIELCNKRIKEYTQQIKDKKQLVEQTEKTVEERTHDLNGKKQ
                     ELENITQETQKEEDELYRLSEEYAMNIEPRLL"
     gene            complement(579..1673)
                     /locus_tag="PLW22_08155"
     CDS             complement(579..1673)
                     /locus_tag="PLW22_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOU74716.1"
                     /translation="MTVREVTSILESFAPLAFQEDYDNSGLIVGDANMEVTGVLLTID
                     VTPQVVAEAVAGGANLIVAHHPVIFKGLKRITGKSYVEQVVVNAIKNNIAIYAAHTNI
                     DSVQGGVSFKMAEVLGLTSTHVLSPLTGQLVKLVTFVPTEYAEAVRQAMFNAGAGVIG
                     NYDSCSFNIQGQGSFRAGDGTNPFVGEKGTIHFEPEFRVETVVPRYKLNAVINAMVEA
                     HPYEEVAYDVYPLDLPYKKAGLGVVGDLPEPMNTIDFVKHVKQLFRVPCVRYTNPVKD
                     FISRVAICGGSAISLLNDAIAANADVFITADVKYHQFFDAENRIVIIDIGHFESEQFT
                     VDIFYDLLSKKISNFAVLKSKVRTNPINYI"
     gene            complement(1680..2018)
                     /locus_tag="PLW22_08160"
     CDS             complement(1680..2018)
                     /locus_tag="PLW22_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU74717.1"
                     /translation="MPYKEKKIEKYYYSIGEVAEMLGVNTSLIRFWEKNFDVIKPHKN
                     KKGNRYFTKNDIENLKLIYHLVKEQGMTLEGARKKLSAQKEELTDNYKIVECLTEIKG
                     MLIEIRDLLD"
     gene            complement(2034..3005)
                     /locus_tag="PLW22_08165"
     CDS             complement(2034..3005)
                     /locus_tag="PLW22_08165"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU74718.1"
                     /translation="MVKHKYKFHPEQLIFVRERKTIRDHLFRAAKYLLATFVIGFISY
                     LVFPIFIDTPEVRKLKRENQELLTNYELITRRIEQLNSVINNLQRRDEGIYRIIFEAE
                     PIPASIREAGIGGVNRYAELEKLSNSKLIIETTKKVDALTKKAYIQSRSFDEITDLAK
                     RKDEMLRSIPAIMPIRDKELTRVASSFGMRIHPFYKVLKMHNGMDFTAPTGTEIFATG
                     DGVVVDIVYAQRGYGNHVIIDHGFGYSTLYAHMSKVAVRKGQRVRRGMVIGYVGNTGT
                     SVAPHLHYEVHRNGQPVNPIHYYFNDLTPEQYEKLVDIASQPTQSFD"
     gene            complement(3012..3974)
                     /locus_tag="PLW22_08170"
     CDS             complement(3012..3974)
                     /locus_tag="PLW22_08170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU74719.1"
                     /translation="MAKFKYQFNPHTLKFDVIRIPLYKQFAKILIHLGFYAGVFLGLG
                     YAFSVIYDTPVEQSLKRANAEYVLKYELAQKQIFELSNALAVLEAKDNNIYRAIFEAD
                     TIPNTIRRGGYGGVDKYANLRSATNSLTLIKTLRALDELTWRAYVQSRSFDDVAEMAK
                     NKEKMILCVPAIQPINTKDLIRVSSLYGFRRDPINGAVTMHAGIDFVGSVGTPIYSTG
                     DGIVVEAGYSFNGYGNEVIVDHGFGYKTRYAHLSKVLTKVGQRVKRAEKIGLLGSSGK
                     STGPHLHYEVILRGKPVNPINYFNDMTEDDYQNMLKKYSSQFLD"
     gene            4126..6744
                     /gene="alaS"
                     /locus_tag="PLW22_08175"
     CDS             4126..6744
                     /gene="alaS"
                     /locus_tag="PLW22_08175"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOU74720.1"
                     /translation="MTSKELRQKFLDFFAGKSHQIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKDIFLGNAPVKYPRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     REAIDWAWEFLVDVLKIDKNRLYATIFEGSPDEGIERDLEAFECWKKHLPEGRILLGN
                     KKDNFWEMGDTGPCGPCTEIHIDLRDDDERKAIPGEQMVNKGHPQVIEIWNLVFIQFN
                     RKANGSLEPLPAKHVDTGMGFERLAMVVQGKKSNYDTDVFTPIIDRISQMCGIPYGTD
                     PKADVAMRVIADHLRAISFSIADGQLPSNVKAGYVIRRILRRAVRYGYTFLNFREPFI
                     DSLVAVLVEQMGDHYPELKAQQTLITNVINKEEATFLRTLETGIKLLEQLIEKAAKEN
                     RTEISGKDAFVLYDTYGFPLDLTELILREHNLSVNRSEFDAEMATQKERSRADAAVEA
                     DDWINVTNAEKVEFVGYDTLKTDTRIIRMRTVKTKGKEQYHVVLDRTPFYAESGGQVG
                     DSGLLESDSESIKVINTFKENNLIIHLVDRLPQNPNSPFFATVDYEKRIRTANNHSAT
                     HLLHNALRAILGTHVEQKGSLVHPDYLRFDFSHFQKMTDEEIRKVEQRVNKLIRQNIQ
                     LDEHRSVPMEQAQKMGAIALFGEKYGENVRVIKFGESVELCGGTHTKATGNIGYFKIV
                     SEGAIAAGVRRIEAITGEKAEEFVYSQIDTINQVKSTLGNPPNVIQAVSKLVDENNLL
                     KDQLNTLQAEKLKVVKAELKAKATERNGINLICERISIGNADAIKDLAFQLKNEVPNL
                     FLVLGSEADGKALLTIMINETLVEKHGLNAAAIVKEAAKEIQGGGGGQPFFATAGGKN
                     PDGLDKAIVKAKEAIK"
     gene            complement(6807..7382)
                     /gene="rsxA"
                     /locus_tag="PLW22_08180"
     CDS             complement(6807..7382)
                     /gene="rsxA"
                     /locus_tag="PLW22_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOU74721.1"
                     /translation="MEYLIIIIAAIFVNNVVLSQFLGICPFLGVSNKIDTSIGMAGAV
                     TFVIVLATMVTFLIQHYVLNPLGVGFMQTIVFILVIAALVQMVEIMLKKLSPALYQAL
                     GIFLPLITTNCAVLGVAILVIKKNYNLLEGVVFGGATAIGFGLALVIMAGIREQLDLA
                     NVPKGMKGVPIALLTAGILALAFMGFAGIVK"
     gene            complement(7394..7972)
                     /locus_tag="PLW22_08185"
     CDS             complement(7394..7972)
                     /locus_tag="PLW22_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOU74722.1"
                     /translation="MSKLYNLTKGIIKENPIFVIVLGMCPTLATTSSAMNGLGMGLAT
                     TFVLLGSNTVISLIARFIPDKVRIPSYIVVIATFVTIVDLVMQAYTQSLYEKLGIFIP
                     LIVVNCIVLGRAEAFANKNSVFDSILDGIGMGLGFTFALTLLGSVREILGAGSLFDHK
                     FISGDAILVFILSPGAFIALAYLIAIFNRLKK"
     gene            complement(7965..8546)
                     /locus_tag="PLW22_08190"
     CDS             complement(7965..8546)
                     /locus_tag="PLW22_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOU74723.1"
                     /translation="MAKVESNFKNMTATLLVITLVASAALGLVYQSTKEPIALAQQAK
                     INNAIKAVLPEFDNQPGNEAYTVAVDGGELTFYPATKEGQPVGTAVKTFSNNGFAGRI
                     ELMVGFLPDGTINKIAVISHKETPGLGDKMDPKKSNFSLQFEGKNPETFKLKVKKDGG
                     DVDAITASTISSRAYCDAVIRAYNALKEGGKHE"
     gene            complement(8539..9543)
                     /locus_tag="PLW22_08195"
     CDS             complement(8539..9543)
                     /locus_tag="PLW22_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOU74724.1"
                     /translation="MDSKLVVSPSPHVHSGESTSRLMFNVVLALMPAFAVSLYYFGVG
                     ALIVTLLSVLSCVLFEYLIQKFILKVQPTITDGSAIVTGLLLAFNVPSNLPWYIVVIG
                     SLFAIGVAKMSFGGLGSNPFNPALAARVFMLISFPVQMTSWPLPIESRLKYIDAITGP
                     TPLAILKEGLKKGDTISQIADQIPSHLQLFMGVMGGSLGEISALALLIGFAWLLYKKV
                     ITWHIPISIFTTIFVFTGILWLINPEQNADPIFHLVTGGVMIGAIYMATDYVTSPMHP
                     KGMLIFGIGIGLLTVIIRVWGAYPEGVSFAILIMNAFVPLINRYVKPKRFGEEVKRNG
                     "
     gene            complement(9575..10906)
                     /gene="rsxC"
                     /locus_tag="PLW22_08200"
     CDS             complement(9575..10906)
                     /gene="rsxC"
                     /locus_tag="PLW22_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOU74725.1"
                     /translation="MLKTFAKGGVHPPENKFSADRPIEVLPLPAKVSIPVSQHIGAPA
                     TIVVAKGDKVKVGQLIAQSAGFVSANIHSSVSGTVESVDPVLDSSGYKRQAVTIVVEG
                     DEWEPTIDRSPELKRTIDADAQTIIKKIAEAGIVGLGGATFPSHVKLSVPTGKKADVL
                     IINGVECEPYLTSDHRLMLEKGHELMVGIQLLMRALGVDKAMIGIENNKPDAIKYLSD
                     ISKDYQGISVHALKVKYPQGGEKQLIKALINREVPSGGLPIDIGAVVHNVGTAFAVYE
                     AVQKNKPLFERVVTVTGKNLAKPSNFLVRIGTPVSDLIAAAGGLPDDTAKVVNGGPMM
                     GKAVTDISVPVTKGTSGIIVFPANESRRKADSVCIRCAKCVTACPMGLEPYLLIRMSQ
                     RMMFDKMEHERVLDCMECGSCSFECPANLPLLDYIRLGKSEVNKIIRARKN"
     gene            complement(10935..11801)
                     /locus_tag="PLW22_08205"
     CDS             complement(10935..11801)
                     /locus_tag="PLW22_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOU74726.1"
                     /translation="MNSIIITAVLVLSLLGVALAIVLYLVAQKFKVIEDPRIDLVEAV
                     LPGANCGGCGYPGCRGFAEAFVKTDDIGSMFCPVGGNDTMAKAAEVLGQTAEAKDPMI
                     AVVRCSGSPEQRKRLVEYDGASSCKLATALYGGDTGCQYGCLGLGDCVAVCKFDAIYI
                     DPVTLLPVVDEEKCTACGACVKACPKFIIELRKKGPKGKRIFVSCINKDKGGPARKAC
                     QVACIGCGKCVKVCPHDAITLENNLAYIDYNKCKLCRKCVEECPTGAILEINFPPRKQ
                     KVEGQAENVENN"
     gene            complement(11820..12242)
                     /locus_tag="PLW22_08210"
     CDS             complement(11820..12242)
                     /locus_tag="PLW22_08210"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOU74727.1"
                     /translation="MSLKPNVIEHLGRVEAITATDIRVTIISQSACASCQAKGACSAA
                     DVAEKQVVVSKPYHNYTVGETVKVTMQQSLGFKALFLGYILPLIVLVVSLFVFNALGF
                     SESVSGLLSLAVLVPYYLMLYLFRKSIEKQFHFDIEKL"
BASE COUNT         3580 a         2851 c         2700 g         3333 t
ORIGIN      
        1 agcaatcggg gttcaatgtt catggcatac tcttcgctca ggcggtaaag ttcatcctcc
       61 tctttctggg tttcctgggt gatattctcg agttcctgtt tcttaccgtt caaatcgtgt
      121 gtacgctctt caacggtttt ctcggtttgc tcaaccagtt gctttttatc ctttatctgt
      181 tgggtgtact ccttgatgcg cttgttgcaa agctcaattt caagggtttg atattcaatc
      241 tccttggtaa gtgagtcgta ctccctgttg ttgcgaacgt ttttctgctg ctcctggtac
      301 ttcttgataa gagcctcagc atccttgatt tcaatttttt tctggttgat ttgggtctcc
      361 aaatcttgga tctctttctt gtagttatcg atacgggtag taagacccac aatttcatct
      421 tccagatcct gaacctcaag gggtagctcc ccgcgtagca atttaatctt atcaattttg
      481 gaatctacct gctgaagctt gtaaaggaaa cggagttttt cctcaatgga cagttcaatg
      541 ccctgatcag ccggtttgat cttctcttta gccataatct atatgtagtt aataggatta
      601 gttcgtactt tggattttaa gactgcaaaa ttagatattt tttttgagag taaatcataa
      661 aaaatatcaa cggtaaattg ttcgctttca aaatgtccga tatcaatgat tacaatccga
      721 ttttcggcat caaaaaactg atggtatttg acatcggcgg ttataaaaac atcggcattg
      781 gcagcaatag catcatttaa aagcgaaata gcgctacccc cgcaaatggc aactctggaa
      841 ataaaatcct taacaggatt tgtgtaccta acgcaaggga ctctgaatag ttgctttaca
      901 tgcttaacga aatcgatagt attcattggt tcaggcaagt cgcctaccac tcccaatcca
      961 gcctttttat agggtaaatc gagggggtaa acatcgtagg caacctcctc gtaggggtga
     1021 gcctcaacca tggcattaat taccgcgtta agtttataac ggggcacaac ggtctcaacc
     1081 ctaaactcgg gctcaaagtg tattgtgccc ttttcgccaa cgaacggatt tgtgccatcg
     1141 ccagccctga aggaaccctg cccttgaatg ttgaatgagc aagaatcgta gtttccaata
     1201 acccctgcgc cagcattgaa catagcctgc cgaacagcct cagcgtactc agtaggaaca
     1261 aaggtaacca gctttacgag ttgaccggta agcgggctta acacgtgggt gctggttaag
     1321 cctaaaactt cagccatttt gaagctaaca ccaccctgca cgctgtcgat attagtgtga
     1381 gcggcgtaaa tggcaatatt gttttttatg gcattaacca caacctgttc aacgtaactt
     1441 ttgccagtta tgcgcttaag ccccttaaaa attacagggt gatgggccac tatcaggttt
     1501 gcccctcctg caacagcctc agcaactact tggggtgtaa catcaattgt aagcaaaaca
     1561 ccagtcacct ccatgttagc atcgccaaca ataaggccgg agttatcgta atcttcctga
     1621 aaagcaagcg gagcaaacga ttccaggatt gaggttactt ctctaacagt cataaacgct
     1681 taatcgagca aatcgcgaat ttcaatgagc atgcccttga tctcggtgag gcactccaca
     1741 atcttgtaat tatcggtaag ttcctccttt tgagcggaaa gctttttacg ggcaccttca
     1801 agggtcattc cctgttcctt aaccagatgg taaatcagtt tcaggttttc gatatcgttc
     1861 ttggtaaagt agcgattgcc ttttttgttc ttgtggggct ttattacatc aaaatttttt
     1921 tcccagaagc ggattaacga ggtgtttacg cccaacatct cggccacttc gccaatggag
     1981 tagtagtact tttcaatttt tttctctttg tatggcattt tagtaaggat tggctaatcg
     2041 aaactttgtg tgggttgcga ggcaatatca accagtttct cgtattgctc gggtgttaaa
     2101 tcgttgaagt agtagtgaat ggggttaacg ggctggccat tccggtgcac ctcgtagtgt
     2161 aaatggggag ccaccgatgt tccagtgttg ccaacatatc caataaccat accgcgccta
     2221 acacgctgcc cctttcgtac ggcaaccttg ctcatgtgag cgtaaagggt tgaataacca
     2281 aagccgtggt caataataac atgattgcca tagccacgct gagcataaac aatatcaacc
     2341 actaccccat cgccagtagc aaatatttcg gttcctgttg gggcggtaaa gtccataccg
     2401 ttatgcattt taagcacctt atagaaagga tgaatgcgca tgccaaacga tgaggccaca
     2461 cgggttagct ccttatcgcg aataggcata atggcaggga tgctgcgcag catctcgtcc
     2521 ttgcgttttg ccaaatcggt tatctcatcg aatgagcggc tttggatgta tgcttttttg
     2581 gttagggcat caaccttctt ggtggtttca ataatcagct tgctattgga tagcttctcc
     2641 agctcagcat aacggtttac accacctata ccggcctcgc gaattgaggc aggaatgggt
     2701 tcggcctcaa aaatgatacg gtagataccc tcatcgcgac gctgtaaatt gttgattacg
     2761 gagtttagct gttcaatacg acgggttata agctcgtagt tagtaagcag ctcctgattt
     2821 tcgcgcttta gcttacgaac ctcaggggta tcgataaata ttgggaaaac taagtaggaa
     2881 ataaaaccaa ttacaaaggt ggccagtagg tattttgcag cacggaaaag gtgatctctt
     2941 atggttttcc tttccctgac aaagatcagc tgttccggat gaaatttata cttatgcttt
     3001 accatatagc cttaatccaa aaattgactt gaatacttct tgagcatatt ttggtagtcg
     3061 tcctcggtca tgtcgttaaa gtagttgatt ggattcacgg gtttaccacg gagaataacc
     3121 tcgtaatgga ggtgtgggcc agtgctttta cctgaactac ccagtaatcc tatcttctct
     3181 gcccgtttaa cccgttgtcc aacctttgtc agcaccttac tcaggtgggc atagcgtgtt
     3241 ttatagccaa agccatggtc aactataacc tcgttaccat agccgttgaa ggagtaaccg
     3301 gcttcaacta caataccgtc gccggtggaa tagataggag taccaaccga gcccacaaaa
     3361 tcaattcccg catgcatggt aacagccccg tttatgggat cgcgacggaa accgtacaag
     3421 cttgaaaccc taatcagatc ctttgtgttg atgggctgaa tggcaggaac gcataaaatc
     3481 attttttcct tattcttggc catttcagca acatcatcaa aagagcgcga ttgaacgtag
     3541 gctcgccagg taagctcatc gagcgccctt agagtcttta taagggttaa tgagttggtg
     3601 gctgaacgta ggttagcata cttatccaca ccgccataac cacccctacg aatggtgtta
     3661 ggaatggtat cggcctcaaa aatagcccgg taaatattat tgtccttggc ttcaagtacc
     3721 gccaaagcat tgctcagctc aaatatttgc ttttgagcca actcatattt aagtacgtac
     3781 tcagcattag cccttttaag cgactgctca accggagtat cgtatataac agaaaaagca
     3841 tagcctaacc ccaaaaaaac acctgcataa aatcccaaat gaattagtat ctttgcaaac
     3901 tgtttgtaaa gaggtattcg aataacatcg aatttgagtg tatgggggtt aaactggtat
     3961 ttaaattttg ccatgtcctt taacacaaac tgttacaatg taacacactt ttgacaaact
     4021 gcaacaaaac tatggtaaaa atatttttta cacaagtaaa aagaacaatt tttttacttt
     4081 aaatagttaa atagagaata ttaacatttt caatactaac tttttatgac ttcaaaggaa
     4141 ttgcgtcaga aatttttaga cttttttgct ggcaagtcgc accaaattgt gccttcggca
     4201 ccaatggtaa ttaaggatga cccaacgctg atgttcacca atgcaggcat gaaccagttt
     4261 aaggatattt tccttggcaa cgcaccggtt aagtatcccc gagtagccaa ctcgcagaaa
     4321 tgcctcaggg tatcgggcaa gcataacgat ttggaagagg ttggtcatga tacctaccac
     4381 cacaccatgt ttgagatgct tgggaactgg tcgtttggcg attactttaa acgcgaagcc
     4441 attgattggg cctgggagtt tctggttgat gtgcttaaaa tagataagaa caggctttac
     4501 gcaacaatat ttgagggtag ccctgacgaa ggaattgagc gcgaccttga ggcttttgag
     4561 tgctggaaga aacacctgcc cgaaggcaga atccttcttg gaaataaaaa ggataatttc
     4621 tgggaaatgg gcgatacagg accttgcgga ccctgtaccg agattcatat tgatttacgc
     4681 gatgatgatg agcgtaaggc tatacccggt gagcaaatgg ttaataaggg gcatccccag
     4741 gtaattgaaa tctggaacct tgtattcatc caattcaacc gtaaagccaa tggttcatta
     4801 gagccacttc cggccaagca tgttgatacg ggtatgggct ttgaacgttt ggccatggtg
     4861 gttcagggca aaaagagcaa ctacgataca gatgtattca cgccaattat tgaccgtata
     4921 tcgcaaatgt gcggaatacc ctatgggact gatcctaagg cagatgtggc catgcgcgtt
     4981 attgctgacc acctcagggc aatcagtttt tcaattgccg atgggcagct accatccaac
     5041 gttaaggctg ggtatgtaat acgccgcatt ctccgtaggg cggttcgata cggttacaca
     5101 ttcctgaact tccgcgaacc gtttatcgat agccttgtgg ccgttctggt tgaacaaatg
     5161 ggcgaccact accctgaact taaggctcag caaaccctca tcaccaatgt aatcaacaag
     5221 gaggaggcaa cattcctgcg cactcttgaa acgggtatca agctgcttga gcagcttatt
     5281 gaaaaggctg caaaggaaaa cagaaccgag atatcgggta aggatgcctt tgtgctctac
     5341 gatacctacg gttttccgct cgaccttact gaactcattt tgcgtgagca taacctgagc
     5401 gttaaccgct cagagttcga tgccgagatg gctacccaaa aggagcgctc cagagctgac
     5461 gctgcagttg aagctgacga ttggattaac gtgaccaatg cggaaaaggt agagtttgtt
     5521 gggtatgaca ctcttaaaac cgacacccgc attatccgta tgcgaacggt taaaaccaag
     5581 gggaaagagc agtaccacgt tgtgctcgac cgcactcctt tttacgctga atcgggcggt
     5641 caagtaggcg attcgggatt acttgagagc gactcggaat ccattaaggt gattaacacc
     5701 tttaaggaga acaaccttat catacatctt gtagatagat tgcctcaaaa ccccaactcg
     5761 ccgttctttg ccacagttga ttacgaaaaa cgaattcgaa ctgctaataa ccactcagcc
     5821 acacacctgc tacacaatgc gctacgcgca attctgggca cccatgtgga gcaaaaaggt
     5881 tcattggttc accccgatta cttgcgtttc gacttctcac acttccaaaa aatgaccgat
     5941 gaggagatcc gtaaggtaga acaacgggta aacaagctta tccgacaaaa catacagctc
     6001 gatgaacacc gaagtgttcc catggagcag gctcaaaaaa tgggagccat tgccctattt
     6061 ggcgaaaagt atggcgaaaa tgtaagggtt attaagtttg gcgagtcggt tgaactttgc
     6121 ggtggaaccc acaccaaggc aaccggtaac attggttact tcaaaatagt ttcggagggc
     6181 gccattgcag ccggggtacg ccgtattgaa gcaattaccg gcgaaaaagc cgaagagttt
     6241 gtttactcac agattgatac aatcaaccag gtaaagtcaa ctcttggcaa tccaccaaac
     6301 gttattcagg ctgtatccaa actggttgat gaaaacaacc tgctaaaaga tcagctaaat
     6361 accctacaag ccgaaaaact aaaagtggtt aaagctgaac ttaaagcaaa agctaccgag
     6421 cgtaatggta tcaacctgat ttgcgagcgc ataagcatag gtaatgccga tgccataaag
     6481 gatttagcat tccagctgaa aaatgaggtg ccaaaccttt tcctggtatt gggttctgaa
     6541 gccgatggca aagcgctgct taccattatg ataaacgaaa cgcttgttga gaagcatggc
     6601 ctaaatgcag ctgctattgt taaggaagcc gctaaggaaa tccaaggagg cggtggcgga
     6661 cagccattct ttgccacagc tggaggtaaa aaccccgatg gattggacaa agcgatagta
     6721 aaagctaagg aagcaataaa gtagaaatca taaaggctgc cccaaataag gcagccttta
     6781 atttattgtc aaaaaactaa acgattctac ttaacaatgc ctgcaaagcc cataaaggcc
     6841 agagcaagaa tacctgcggt gagtagagca atgggaaccc ccttcatccc tttaggaacg
     6901 tttgctaaat cgagctgttc acggattcca gccattatta caagtgctag tccaaaacca
     6961 attgctgttg cacctccaaa aacaacccct tcaagcaggt tatagttctt tttgataacc
     7021 agaatggcta caccaagaac agcacagttt gtagtgataa ggggaagaaa aatacctagg
     7081 gcctggtata atgcagggct caacttcttc agcataatct ccaccatttg caccagagca
     7141 gcaattacca gaatgaacac aatggtttgc ataaaaccaa ctcccaaagg attcagtacg
     7201 taatgctgaa tgaggaaagt aaccattgtt gccagtacaa ttacaaaggt aacggcaccc
     7261 gccataccaa tagatgtgtc tatcttattt gataccccca agaaaggaca aataccaagg
     7321 aattgcgata gaacaacgtt gtttacaaat atggcggcaa ttataattat tagatattcc
     7381 atactgtttc ctcctatttc ttgagacggt taaaaatggc aatcaggtag gcaagggcaa
     7441 taaatgcacc cggcgaaagg ataaatacaa gaattgcatc gcccgatata aacttatggt
     7501 caaacagtga gcctgcacct aggatttccc ttacagagcc taaaagagtt aaggcaaatg
     7561 taaagcccag ccccatacca ataccatcga gtattgagtc aaatacgctg tttttgttgg
     7621 caaaagcctc ggcacgacca agaactatgc agtttacaac aataagaggg atgaaaatac
     7681 caagtttctc gtaaagcgac tgggtgtagg cctgcataac caaatcgaca atggttacaa
     7741 atgtagcaat tactactatg tacgaaggaa tacgcacttt atcgggaata aagcgtgcaa
     7801 taagcgagat taccgtattg gaacctagca agacaaaggt tgttgcaagt cccatcccca
     7861 atccattcat ggctgaggac gtggtagcca gggtaggaca catcccaaga acaattacaa
     7921 atatgggatt ttcctttatt atccctttag tcaggttata tagcttactc atgcttacct
     7981 ccttccttta aagcgttata tgcacggatt actgcatcgc agtaggcgcg gctcgaaatg
     8041 gttgaagcag taatagcatc aacatctccc ccatcctttt taaccttaag cttaaatgtt
     8101 tcggggtttt tcccctcaaa ctgaaggcta aaatttgatt ttttggggtc catcttatcg
     8161 cctaaaccgg gagtttcctt gtgtgagata acagcaatct tattaatggt tccatcggga
     8221 aggaaaccaa ccatgagttc aattcgacct gcaaaaccat tgttgctaaa ggtttttact
     8281 gccgttccta caggttgccc ttcctttgta gccggataaa aggtaagttc gccaccatca
     8341 acagcaacag tgtaagcctc gtttccgggt tgattatcaa actcggggag tacagcttta
     8401 atggcattat ttatttttgc ctgctgcgca agggcaattg gctccttggt tgactgataa
     8461 acaaggccaa gcgccgcaga ggctaccagg gtaataacca gcagggtagc cgtcatattc
     8521 ttgaaattcg attccacctt agccattgcg ctttacctcc tctccaaaac gtttaggttt
     8581 aacatatcgg ttaattaatg gcacaaaagc gttcatgatt agaatggcaa acgaaacgcc
     8641 ctctgggtat gctccccata ccctaataat cactgttaac aaccctatac caataccaaa
     8701 aatcagcatt ccttttgggt gcatgggtga ggtaacataa tcggttgcca tgtatatggc
     8761 accaatcatt acacctcctg taaccaagtg gaaaattggg tcggcattct gttcagggtt
     8821 tattagccat aggatacccg tgaaaacaaa aatggttgtg aaaatagata ttggaatatg
     8881 ccaggttatc acctttttat agagtaacca cgcaaaacct atgagtagag ccaaggctga
     8941 tatttcgcca agcgaaccac ccataacccc catgaaaagc tgaaggtgcg agggaatttg
     9001 gtcagcaatt tgcgaaatgg tatcaccctt ttttaaccct tccttcagta tggccagggg
     9061 tgttgggcct gtaatagcat cgatatactt taaacgcgat tcaattggca gtggccaact
     9121 ggtcatctga accgggaagg atatcagcat gaaaacgcga gccgctaaag cggggttgaa
     9181 cggattgctt cccaacccac caaagctcat tttggcaact ccaatggcaa ataagctacc
     9241 aattacaaca atgtaccagg gcaagttgct tggaacgtta aaggcaagca gcaaacctgt
     9301 aacaatggct gaaccatcgg taatggtagg ctgaacctta aggatgaact tttgaataag
     9361 atattcaaac agaacacatg agagtaccga gagtaatgta actattaggg cacctacgcc
     9421 aaagtagtaa agtgaaaccg caaaggcggg cataagcgcc aataccacgt tgaacataag
     9481 tcggctggtc gattcgccgc tatgcacgtg gggtgatggt gaaactacaa gtttactatc
     9541 cattatcttt ttaagtttgg ctggttatgt caaattagtt ctttcgagcc ctgatgattt
     9601 tattcacttc gctcttacct agcctgatgt aatcgagcaa aggtaggttt gctgggcact
     9661 caaagctgca cgatccgcac tccatacaat cgagcacacg ctcatgctcc attttgtcga
     9721 acatcatacg ctggctcata cgaatgagta agtatggttc aagccccatg ggacaggctg
     9781 taacacactt ggcgcagcgt atacatacgc tatcagcctt acgtctcgat tcgtttgcag
     9841 ggaaaacgat tatacccgat gtacccttgg taaccggaac agaaatatcg gttaccgctt
     9901 tacccatcat ggggccgccg ttaaccacct tagcagtatc gtcgggtaaa ccaccggcag
     9961 cagctatcag gtcagaaacc ggtgtcccaa ttcgaactaa aaagtttgat ggtttggcta
    10021 agttcttccc ggttacggta accacgcgct caaaaagggg cttattcttt tgaactgcct
    10081 cgtaaaccgc aaaagctgta cccacattgt gtacaactgc acctatatct ataggtaagc
    10141 cacccgatgg aacctcgcgg ttgatgagcg ctttgataag ctgcttttca cccccttgag
    10201 ggtattttac ctttagcgca tgaacgctaa tgccttggta atcctttgaa atatcggaaa
    10261 gatatttaat ggcatcgggc ttgttattct caatgccaat cattgccttg tcgacaccta
    10321 aggccctcat gagtaactga atacccacca tcagctcatg ccccttttcg agcatcaagc
    10381 ggtgatccga tgtgaggtat ggttcacact caacaccgtt aattatgagc acatccgctt
    10441 ttttgccggt tggaaccgaa agtttaacat gcgatgggaa tgttgctccg ccaagaccta
    10501 caataccagc ctcggctatt ttcttgatga tggtttgcgc atcggcatcg attgtgcgct
    10561 ttagctcagg cgatctatca attgtgggtt cccactcatc gccctcaact acaattgtta
    10621 cagcctgacg cttgtagcca gaactgtcga gaactggatc aaccgattca actgtacccg
    10681 acaccgatga atgaatattg gccgagacaa atcctgcgct ttgagcaata agctgaccaa
    10741 ccttaacttt atcgccctta gcaaccacta tagttgcagg cgctccaatg tgctgcgata
    10801 caggaatgct tacctttgca ggtagcggca acacctcaat gggcctatcg gccgaaaatt
    10861 tattctcagg cggatgaacg cctcctttag caaatgtctt caacattact atatggttat
    10921 gttaggtgtt acaattaatt attttctaca ttctcagctt gcccttcgac cttttgctta
    10981 cgtggtggga agtttatttc aaggattgca ccagtaggac actcctccac gcacttacgg
    11041 caaagcttac acttgttgta atcgatatag gcaaggttat tttcaagggt gatggcatcg
    11101 tgggggcaaa ccttaacaca cttaccacag ccaatgcatg ccacctggca agccttacgg
    11161 gctgggccac ccttatcttt gttaatacac gatacaaaga tgcgcttacc cttgggcccc
    11221 tttttgcgaa gctcaatgat gaactttgga cacgccttaa cgcaagcacc gcaagcggta
    11281 cacttctcct catcaaccac gggtaaaagg gttaccggat cgatataaat ggcatcgaac
    11341 ttacaaacgg ctacgcaatc gcctaagcca aggcaaccat actggcaacc tgtatcgcca
    11401 ccatataggg ctgtggccag cttacacgat gatgcgccat cgtattccac aaggcgtttc
    11461 cgttgctctg ggctacctga gcatcgtaca acggcaatca tgggatcctt tgcctcggcg
    11521 gtctggccaa gaacctcggc agccttagcc atagtatcgt taccacccac agggcaaaac
    11581 atcgatccaa tatcgtcggt tttaacgaat gcctcggcaa agccccggca accgggatat
    11641 ccacacccgc cacagttagc cccgggcagt acggcctcaa ccaaatcgat acgcggatcc
    11701 tcaattacct taaatttctg agccacaagg tataacacta tggcaagcgc cacacctaaa
    11761 aggctaagaa ctaaaactgc tgtaataatg atagaattca tagattaggt tggttttact
    11821 tataatttct caatatcaaa atgaaactgt ttctcaatac ttttcctaaa taagtaaagc
    11881 ataaggtagt agggtacaag aacagctaac gagagcaaac ccgatactga ctcgctaaac
    11941 cccaaggcgt tgaacacaaa aagggaaaca acaagaacga ttaacggaag gatgtatccc
    12001 aaaaataaag ccttgaaacc aagcgattgc tgcatggtaa ccttaacggt ttcgcctacg
    12061 gtgtaattat ggtatggctt tgatactact acctgtttct cggcaacatc ggctgctgaa
    12121 caggcccctt tagcttgaca gctggcgcaa gcggactggc tgattattgt tacccgaata
    12181 tcggttgcag ttattgcctc aacacgtcct aaatgctcaa taacattggg ttttaaagac
    12241 atctacggaa aacgtttgaa atcaaccaca aaaataattt tctttagtat acaattcagc
    12301 cgtaaatgca atttggaatc attctaaaca tttgcaaaat aataatcaat ttcgattaga
    12361 tagccaaaca gactctcctt ttgtcatcct gagcgtagcg aaggatctat tgaatagtgt
    12421 gcagtgtaca ggggttttta gtgtttggtg tttggtgttt agtg
//
LOCUS       DAOOWJ010000076        12307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5136_length_12307_cov_7.711312, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000076 DAOOWJ010000000
VERSION     DAOOWJ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5136_length_12307_cov_7.711312"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..477
                     /locus_tag="PLW22_08215"
                     /pseudo
     CDS             250..477
                     /locus_tag="PLW22_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794072.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(646..2997)
                     /locus_tag="PLW22_08220"
     CDS             complement(646..2997)
                     /locus_tag="PLW22_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU74728.1"
                     /translation="MKRNYYRSNFILSVSAAVLTAIIFIFLYYTIGVRQRRFIYNDSK
                     WIAIQQSKNAASQVESYFINAIHSARSLVKQTKVLYEKRADRSEIVSLIHSYTKDNPD
                     FLAVWTMWEPNAFDGKDKQYRNTPPYDREGHLSVTFFRMADSIVFEYTEPEEYNEFYY
                     TVPKERNSEMVLEPYLYQYQGHPIEFYETSIVVPIIINNKFCGVYGIDLDLNYLQRKL
                     SEIKLQTKGHVSLISSDGEIVTHPDTAFIGKNIFSLAGTNDTLARYIVKSGLEQNYET
                     VCNFTKSEVFRTFYPIRVGKSDTQWSIMVQLDKREATLRSISLHNIALGLLIAGLILI
                     SYLIYNIFDRLRYEHQLILAKESAEQKERELQKMYYELQTSEEELRATNEELFATSEM
                     LRESNLMLTGAKEKAEEASRLKTAFLHNLSHEVRTPLNAIYGFSQMLNNIDISADESK
                     KFIQIIQDSSKQLVSIISDIITISSIETGQERITNTNIHLNTLLDELYQTFSAKLANK
                     PVELRVYKEFSDNEDEIFVDDLKLRQVLHHLLLNAVKFTHNGSIEFGYRLKSSNLEFY
                     VKDTGIGIEGKKQDVIFERFTQADENIHAEYGGTGLGLSICKGFLNLMGGAIWVESEL
                     GQGATFYFTLPYHPITSKESKDISIQSQTNNSEMKTILVAEDDINSFKLLKAVLKDFD
                     FQLLHADNGRKAVELCQNEDSICLVLMDIKMPELNGVSATKLIKELKPTLPVIAQTAY
                     ATLDDIEKNREVFDAYITKPLDIEQLKKIILEFCDRFGKSEKY"
     gene            complement(3023..3349)
                     /locus_tag="PLW22_08225"
     CDS             complement(3023..3349)
                     /locus_tag="PLW22_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOU74729.1"
                     /translation="MSFARVYPLYLTKIERKGRTREELNVIIKWLTGFDTATLESLIE
                     QNVTFEEFFRLATINPNAGLITGKVCGIKVEEIKNPLTQKIRYLDKLVDDLAKGKSIE
                     KIIPKP"
     gene            complement(3742..4197)
                     /locus_tag="PLW22_08230"
     CDS             complement(3742..4197)
                     /locus_tag="PLW22_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU74730.1"
                     /translation="MKEFSNINDILDFAIAREQDAVDFYNRLAAKARNNDMRHIFEQF
                     AREEMMHKARLAKIKEEGVITLPTEKVVDLKIADYVVLDSERDDMTYEQALVLAMKRE
                     KAAFKLYLALSEKVDKTEYKELFKQLAQEESRHKLRFELEYDEYVLKDN"
     gene            complement(4175..7888)
                     /locus_tag="PLW22_08235"
     CDS             complement(4175..7888)
                     /locus_tag="PLW22_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU74731.1"
                     /translation="MSELVNIVLNGKNTTASKGEYILDVARRHGIEIPTLCNDPRLEP
                     YSSCYVCVVEIEGQRNLQPSCSTRVAEGMKIITDNERVYKARKTALDLIMSNHYADCV
                     APCRERCPAGVDVQGYISLIEKGLYSEAIGLIKQVNPLPAICGRVCVRPCEAACRRNY
                     MDEGNGVGIDYLKRFAADRDMESEHHYKPTIAPSTGKKVAIIGAGPGGLSAAYFLQQK
                     GHQCDIYEAQPHAGGWLRYGIPEYRLPNDILDKEVSTITELGVKIFYNQRLGKNLSYK
                     TIAENYDATILTIGSQRGQLLGAEGDDADGVFSGIDFLRNMEVTGQRYDFKGKRIAVV
                     GGGNTAMDCCRTSIRCGSTDVTVIYRRTEAEMPANPIEIHESKLEGVKYMFLTTPVRV
                     NKTADGKVKSMTLIRMELGEPDASGRRRPVPVEGSEFEMEFDYILAAIGQKTEVDFLD
                     DINKHSRHGELKVTKWGDIEADRQTLQTGIPSVFAAGDGVTGPATIIEAIAQAKLASH
                     SCHLYLTGQPVVPPRKEFLSKKDNFRKLSSNDFVTRYQKQQREEMPVMDANQRVNFKE
                     VELGYTHEQAMHETARCLECGCVEYFECDLKRHADTYQADQLRFMGDHKEFDVNFTHP
                     FIEIDNNKCILCSRCVRVCKEIVGANALTLVKRGFDTFVAPAFGDNLADTTCESCGLC
                     ISACPTGAITENVPFKPGPVKTDKFETICGYCSVGCTLNFHHKSGYLMRVTGANGQVN
                     TDANLCMYGKFGYREFNSINRLTKPLKKEGDKFVEISYQEAFDIITQKIKSVAPDANA
                     FFAGAILTNEEQYLVQKLARTGAKTNNVGSFHYLGTGNGFTSDSIQSSMFGDISKANH
                     IYLFATQINRYNAVAGYMVNASKKPVTVIAKEESNLSNRSKEFIKVDSYYHFIKAVNH
                     YLLSGNKQNMVFIGDHCEGFGAYSANLLLEDYANLCEQAGCERSVIERFADEFNNDYG
                     ALLIFAEAEVSDNTAAEIMNLMLITGKLGKTAGGLIALKPKNNSHGLSDMGLNPNWGL
                     GLQDITNQEFADKLKRKWGVESLSSKKYNLVELLEKGSLSNILIVGEDPIGTAKHKAA
                     VKDYLQKVRFKVVIDAFLTETAQIADIVLPASLWYESGGSFTNTNHTIQQFEVGVKPK
                     VELTTADLLTKLLNRFGVNSPTDIYDIQTEILSLLPQTESGKHLQFTQTSSEGQHLQF
                     HAGCSYLMHNFDVEFENKLINAKTHSHERVFQH"
     gene            complement(7927..9705)
                     /locus_tag="PLW22_08240"
     CDS             complement(7927..9705)
                     /locus_tag="PLW22_08240"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOU74732.1"
                     /translation="MAETKVIVGLGSCGIAAGANKVYNRLKALKEADNLDFELAKTSC
                     IGMCFREPLVEVVDENGSMIYGDVDEDKAIEIIQQHVLGHNPIKEYVVKTDLFPTPDQ
                     SYFDGQVKIALRNCGYIDPENIHEYEARGGYQALRKIANEALLPEQIIQTIIDSGLRG
                     RGGGGFPTGMKWRFARANQSEEKYIICNADEGDPGAFMDRSLLEGDPHAVLEGMIIGA
                     YAIGATEGVIYCRAEYPLAIKRLNIALAQAREHGYLGKNIMGIQGFNFDIFIKEGAGA
                     FVCGEETALMASIEGKRGMPSKRPPFPAEKGLWQKPSNINNVETYANVPYIVANGAES
                     YNKYGTEKSKGTKVFALTGKIKHGGLVEVPMGTPLRDIIYKLGGGIQNDKKFKAVQLG
                     GPSGGCIPEHLIDIQVDYEQVTATGAIMGSGGMVVMDETTCMVDVARFFLDFTQKESC
                     GKCTFCRIGTKRMLEILTRITEGEGQEGDIEKLEELALQVRESSLCGLGQTAPNPVLT
                     TIKYFRHEYEAHIRDKKCPAHSCKKLLTYEIDPNKCTGCTVCAVKCPVKAIDGERKKV
                     HHINQDICIKCGDCYNSCRFDAITKS"
     gene            complement(9753..10286)
                     /gene="nuoE"
                     /locus_tag="PLW22_08245"
     CDS             complement(9753..10286)
                     /gene="nuoE"
                     /locus_tag="PLW22_08245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOU74733.1"
                     /translation="MRVQRILTKPASSIPADEVDLALIEPLIKKYKGKKGNLIPLLQG
                     VQSIYGYIPRSAFELIAHETGLTLSDMYGVATFYAQFRLSPVGKHIIKVCHGTACHVQ
                     GANAISTALKEALKVNDGETTPDKLFTLETVACLGCCSLAPVMMIDEETYGKLTGSSA
                     VKIVKDIKIKETQAKGE"
     gene            complement(10503..11858)
                     /gene="gdhA"
                     /locus_tag="PLW22_08250"
     CDS             complement(10503..11858)
                     /gene="gdhA"
                     /locus_tag="PLW22_08250"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOU74734.1"
                     /translation="MKNVALQEKVNNFMAGLIARNPNEPEFHQAAREVIESIMPYIDE
                     NPIYQDEKILERITEPERIVIFRVPWIDDKGNIQVNRGYRIEMNSAIGPYKGGLRFHP
                     TVNLSILKFLAFEQVFKNSLTSLPMGGGKGGSDFDPKGKSDKEVMRFCQSFMTELYRH
                     IGPDTDVPAGDIGVGGREIGYLFGQYKRIVNEFTGVLTGKGLKWGGSLIRPEATGYGN
                     VYFAQEMLKTRGESFKDKIVAVSGSGNVAQYTVEKVNELGGKVVTLSDSDGFIYDPKG
                     IDNEKLKFVMWLKNEKRGRIKEYAEKYGCDYFPAERPWGIKCDIALPSATQNEINGEE
                     ARTLVRNGCFCVSEGANMPSTPEAVDVFLNAKILYGPGKAANAGGVATSGLEMSQNSL
                     RLSWTREEVDQRLHQIMINIHETCRKYGTKEDGFINYVDGANIGGFVKVADAMIAQGV
                     V"
BASE COUNT         3155 a         2723 c         2698 g         3731 t
ORIGIN      
        1 ctaaatctcc cctgagaggg gagactttag ccctgcaata aggtttcctt attgcttctc
       61 ccctctcctg tcaggagagg ggaggggtga ggtcaatttc atgaataacg agttactaac
      121 aacgtttcca gccccgtagg ggcgtaatac ttgtaacaac atgattattc ccacatgtat
      181 agcgatgcac gcattaaatt ttccaaagag caaggggaaa gcgttgcacc gcaacggaaa
      241 agcccggcta acgagaatgc caatgggctg atcaggcagt tctttcccaa gaaaacatcc
      301 tttgaaaaca tcacggatga acaggttaaa tgggtggaag agatcctaaa caacagacca
      361 agaaaacgtt tggggtttct aactcccaaa gaaaaactgg aagaaatttt gtttaacaaa
      421 aaagttgcat ttgcaatgaa aatctatgat atatttatct gctcaattct cctctaactt
      481 cctctgactc cttcatattc tcaattcttc ttttaacttt tcacttttca cgtttaacga
      541 agtcaaacat cttctaactt catcatattc tgattgttcc gaatcattcc gaatcattcc
      601 cctaaagtta gcccagtaga attagttaat acaaagaact taggattaat acttttcgct
      661 tttcccgaat ctatcgcaaa actctaaaat tatttttttt agctgttcaa tatctaaggg
      721 ttttgtgata taggcatcga acacctccct gttcttttca atatcgtcaa gggtggcata
      781 agcagtttgg gctattacgg gtagagtagg ttttagctct ttaatgagct tagtggccga
      841 tactccgttg agttccggca ttttaatatc catgagtacc aggcagatag aatcttcgtt
      901 ttggcaaagc tctacagctt tgcggccatt atcggcgtgt aggagctgaa agtcaaaatc
      961 ttttagcacc gcttttagca gcttgaagct atttatatcg tcctcggcca caagaattgt
     1021 tttcatttcg gagttgttcg tttgtgattg gatactaata tctttactct ctttactggt
     1081 tattgggtga tatggtagtg taaagtaaaa ggttgcccct tgccctagct ctgattcaac
     1141 ccaaatagca ccgcccatta agtttaaaaa gcccttacat attgataaac ccaatccggt
     1201 tcccccatac tcagcatgga tattttcatc ggcctgggta aagcgttcaa aaatcacatc
     1261 ttgcttttta ccttcaattc ctatgccagt atccttgacg tagaattcca ggttgctact
     1321 tttaagccta taaccaaatt caatagagcc attgtgggtg aacttcactg catttaaaag
     1381 caagtggtgt agtacttgtc taagctttag gtcatcgaca aaaatttcat cttcattatc
     1441 ggaaaactcc ttgtaaaccc tcaattcaac tggtttattg gcaagttttg cagaaaaagt
     1501 ttggtaaagc tcatcgagca gggtattcag gtgtatgtta gtgttggtta ttcgctcctg
     1561 tccggtttca atggatgaaa ttgttatgat atcgctgatg atagaaacta attgcttgct
     1621 tgagtcctgt atgatttgaa tgaacttctt agattcgtct gctgagatat caatgttatt
     1681 gagcatttgc gagaaaccat aaattgcgtt taagggtgtt cgcacttcgt gacttaggtt
     1741 atgaagaaat gctgttttga gtctgctggc ctcctcggct ttttccttag ctccggtaag
     1801 cataaggttg ctctcacgta acatttcgga tgttgcgaaa agttcctcgt tggttgcccg
     1861 tagctcctct tcggatgttt gcagttcata gtacattttt tggagttcgc gctctttctg
     1921 ttcagcactt tccttagcta atatgagctg atgctcgtac cttagcctgt cgaaaatgtt
     1981 gtaaatcaaa taggaaataa ggatcaagcc agcaattaaa aggccaaggg caatgttatg
     2041 gagggaaatt gaacgtagag tggcctcacg cttatccaat tgaaccatta ttgaccactg
     2101 ggtgtcgctc ttaccaacac gaatggggta gaatgttcta aaaacctcac ttttagtaaa
     2161 gttgcaaaca gtttcatagt tctgctccag ccccgatttc acgatgtacc tggcaagggt
     2221 atcgttagtc ccagcaagcg agaaaatatt tttgccaatg aatgcggtgt ccggatgagt
     2281 aacaatttcg ccatcggatg aaattagcga cacgtgacct ttggtttgca gttttatttc
     2341 cgataatttt ctttgtaggt agttaaggtc gaggtcaata ccataaactc cgcagaactt
     2401 gttgttgatt attattggta ctacaattga tgtttcgtag aattcaattg ggtgtccctg
     2461 atactgatat aggtatggct ctaaaaccat ttcgctattc ctctctttgg gaacagtata
     2521 gtaaaactca ttgtattctt cgggttcagt gtactcaaac acaatgctgt cggccatgcg
     2581 gaaaaaagta accgatagat gtccttccct atcgtagggt ggggtgtttc ggtattgctt
     2641 atcctttcca tcgaatgcgt ttggctccca catagtccaa accgctaaaa aatcggggtt
     2701 atcctttgtg taactatgta taagactcac aatctccgac ctgtcggctc tcttttcgta
     2761 taaaaccttc gtttgcttta ctagcgaacg ggcggagtgt atggcattga tgaaatacga
     2821 ttcaacctga ctcgcagcat ttttggattg ctgaatggca atccacttgg agtcgttgta
     2881 aataaatcta cgttggcgaa caccaatggt atagtaaagg aaaataaaga ttatggcagt
     2941 tagaaccgcc gccgaaacag atagaatgaa gttggaccgg taatagttcc ttttcatgat
     3001 agattgtgaa aataagtatt gtttatggct ttgggattat cttttctata cttttccctt
     3061 ttgccaaatc atcaaccaac ttgtccaggt agcgtatttt ttgggttaaa ggattcttga
     3121 tttcctctac ctttattccg caaacctttc cggttattaa tcccgcattg gggttgattg
     3181 ttgcaagccg gaaaaactcc tcaaaggtta cattttgctc aatgagactt tccagcgtag
     3241 cagtgtcaaa acccgtaagc catttaatga taacgtttag ctcctcgcga gttcgccctt
     3301 tcctttcaat cttggtaagg taaagggggt aaacccttgc aaatgacatg cttgcaattt
     3361 tgctgtcgga atccatttcc ctttacaatg gtatttgtat aacaagctaa atatatggtg
     3421 atagtaatgc ttttcaaaat ataaatcgaa cttttttgtt ttttttatcc tttggagtga
     3481 ttggttgtgt tatgtgttga taatgagagg tatgaatact ttgtgttgat gtttattttt
     3541 cggtatttag tactaatagt gatagtgaat ttgtagcttt ttgtttacat agcagatgta
     3601 tcctgaattt caaattggtt tgcttaattg tttgttatgc catttgggaa ttcattagta
     3661 ttaatgaatt tgataaagtt tcaaagatat gtgacgaaaa taattaaaaa agccccgcca
     3721 gggggctttt agggatgggt tttagttatc ctttagaacg tattcatcgt attccaactc
     3781 aaaacgtagc ttatggcgcg attcttcctg tgccagctgc ttgaatagtt ccttgtactc
     3841 agttttatca actttttcag aaagggcaag gtatagctta aaggccgcct tttcgcgttt
     3901 catggccaga acaagtgcct gctcgtaggt catgtcgtcg cgttcggagt cgagcacaac
     3961 gtaatcggca atcttaaggt caaccacttt ctcggttggg agggtaataa ctccctcttc
     4021 ctttatcttg gcaagtcgtg ccttatgcat catttcctcg cgggcaaact gttcaaatat
     4081 atgcctcata tcgttgttcc gtgcttttgc ggccaaccgg ttgtaaaaat caaccgcatc
     4141 ctgttcacgg gctatggcaa agtccaaaat atcattaatg ttggaaaact ctttcatggc
     4201 tatgggtttt tgcgttaatc aatttgtttt caaattcaac atcaaagttg tgcatcaaat
     4261 agctgcaacc tgcatggaat tgaaggtgtt gcccctcgct ggaggtttgt gtaaactgta
     4321 gatgtttgcc ggattcagtc tgcggcagaa gcgatagtat ttcggtttgg atatcgtaaa
     4381 tatcggttgg gctattcacc ccaaacctat tcagcagctt ggtaagtaaa tcggcagttg
     4441 ttaactcaac cttaggtttt acacctacct caaactgctg gatggtatgg ttggtgttgg
     4501 tgaacgaacc tccactctca taccaaagcg aagcaggaag gactatatcg gcaatttggg
     4561 ctgtttcagt taaaaacgca tcgattacca ccttaaatct tactttttgt aggtaatcct
     4621 tcacggccgc tttatgcttg gcagttccaa ttgggtcttc gcctacaatg agaatatttg
     4681 ataaacttcc tttttccaaa agttcaacca ggttgtattt tttgctcgat agactttcaa
     4741 ccccccattt tctcttaagt ttgtcggcaa attcctggtt agttatatcc tgtaagccta
     4801 atccccaatt ggggtttaat cccatgtccg atagcccatg tgaattattt ttgggtttca
     4861 aagctattaa gccaccagct gttttgccca atttgccagt aatcagcatt aagttcataa
     4921 tttcagcagc ggtattatcg gaaacctcgg cttcggcaaa aattaacagt gcgccataat
     4981 cgttattgaa ttcatcggca aaacgctcaa taacactccg ttcgcaacca gcctgttcgc
     5041 aaaggtttgc gtaatcttcc agaagtaaat ttgcactgta agcgccaaaa ccttcacagt
     5101 ggtcgccaat aaacaccata ttttgcttgt taccacttag taggtaatgg ttaaccgctt
     5161 taatgaaatg gtagtatgag tctaccttta taaactcttt tgacctattg gacaggttcg
     5221 attcttcctt ggcaattacg gtcaccggtt ttttcgatgc gtttaccatg taaccagcca
     5281 cggcgttgta tcggtttatt tgagttgcaa ataggtagat atggttggct ttgcttatat
     5341 cgccaaacat tgaactctgg atactgtcag acgtaaatcc gttgcctgtg ccaaggtaat
     5401 ggaaactacc aacattattg gtttttgcgc ctgtccgagc aagtttctga accaggtatt
     5461 gctcctcgtt ggttaaaatt gcaccggcaa aaaaggcgtt cgcatcgggg gctacggatt
     5521 taatcttctg ggtgattata tcaaaagcct cttggtagct gatttcaaca aatttgtctc
     5581 cctctttttt tagtggcttg gttaaacggt tgatgctatt gaactccctg tacccaaact
     5641 taccgtacat gcataggttt gcatcggtgt tcacttgccc attagcacct gttacacgca
     5701 ttaggtatcc gctcttatgg tggaagttaa gcgtacagcc cacagagcag tatccacaaa
     5761 tggtttcgaa tttatcggtt ttaacgggtc cgggcttaaa aggtacattc tcagtgatag
     5821 ctccagtagg gcaagcagaa atacacaaac cacacgattc gcaagtggtg tcggccaggt
     5881 tgtcgccaaa ggcgggtgct acaaatgtgt cgaatccacg cttaacaagc gtaagggcgt
     5941 tggctcccac tatttccttg caaacgcgga cgcaccttga gcaaagtata cacttgttat
     6001 tatcaatttc gatgaaaggg tgggtgaagt taacgtcaaa ctccttatgg tcgcccatga
     6061 accggagttg gtcggcctga taggtatcgg cgtggcgttt taggtcgcac tcaaagtatt
     6121 caacacatcc acattctaag cagcgagcag tttcgtgcat ggcttgttca tgggtatagc
     6181 caagctcaac ttccttgaag ttgacacgct gatttgcgtc cattacgggc atttcctcgc
     6241 gctgctgttt ttggtatcgg gttacaaaat cgttgctgga cagcttgcgg aaattatcct
     6301 tcttgctaag gaactccttg cggggtggaa caactggctg tcccgtaagg tacaagtggc
     6361 agctatgcga ggcaagttta gcctgtgcta ttgcctcgat aatggttgca ggaccggtta
     6421 cgccatcgcc tgcagcaaaa accgatggta tgcctgtttg gagggtttgc ctatcagcct
     6481 caatatcgcc ccatttggta accttcagtt caccatgccg ggagtgtttg ttgatgtcgt
     6541 cgaggaaatc aacctcggtt ttttggccaa tggctgccag gatatagtcg aactccattt
     6601 caaactctga gccctcaacc ggaacagggc gtcgacgccc tgatgcatcg ggttcgccaa
     6661 gctccatgcg gataagagtc atcgatttta ccttgccatc ggcggttttg tttaccctaa
     6721 ccggtgtggt taggaacatg tactttacac cttcgagttt tgactcgtgt atttctattg
     6781 ggtttgcagg catttcggcc tcagttcggc gatagataac ggttacatcg gtgctaccgc
     6841 atcggattga ggttctgcag cagtccattg ctgtgttacc accgccaaca acggcaatgc
     6901 gtttaccttt gaagtcgtaa cgctggccag taacctccat gttgcgtagg aaatcgatgc
     6961 cggaaaaaac tccatcggca tcatcgcctt cagcgccaag caactgtccc ctttgggagc
     7021 caatggttag tatggttgca tcatagttct cggctatcgt tttgtagctt aaattcttgc
     7081 caaggcgctg gttgtaaaag atttttacac caagttcggt aatggttgac acctctttgt
     7141 ccagtatatc gttaggtaaa cgatattccg gtattccata ccggagccac ccccctgcgt
     7201 ggggttgggc ttcatatatg tcgcactgat gacctttctg ctgaaggaag tatgctgccg
     7261 acagtccacc gggaccagcc ccaataattg ccaccttttt ccctgtagag ggtgcaatgg
     7321 ttggtttgta gtggtgctcc gattccatat cgcggtcggc cgcaaagcgt tttaggtaat
     7381 cgataccaac cccgttgccc tcatccatgt aattcctacg gcatgcagcc tcacaagggc
     7441 gaacgcatac tctaccacag attgcaggta gggggttaac ctgtttgatt aaaccaatgg
     7501 cctcactgta aagtcctttc tcaatcagcg agatatagcc ctgaacatca acaccagccg
     7561 ggcagcgttc gcgacagggt gcaacacagt cggcgtaatg gttgctcatt attaggtcga
     7621 gcgctgtctt acgcgcctta taaaccctct cgttgtcagt gattattttc atgccttcgg
     7681 ccaccctggt ggagcaagag ggctggagat tccgttgacc ctcaatttca accacgcaaa
     7741 catagcacga cgagtagggc tcaagccttg ggtcgttgca aagggtaggg atttcgatgc
     7801 catgacggcg ggcaacatca agaatatact cgcctttgct tgcggttgtg tttttaccgt
     7861 ttagaactat attgaccaat tcgctcattt caaatcaatt taatgcgttt aatcatcaac
     7921 gtttggttaa ctcttggtaa tagcatcgaa acggcagctg ttgtagcaat cgccgcattt
     7981 aatgcaaata tcctgattga tgtggtgaac ttttttccgt tcgccatcaa tggctttaac
     8041 cgggcatttt acggcacaaa cggtacatcc ggtacacttg ttggggtcaa tctcataggt
     8101 aagtagtttc ttgcagctgt gggctgggca tttcttgtcg cggatgtgag cctcatactc
     8161 gtgccggaaa tacttgattg tggtaagtac agggttaggt gctgtttgac ccaagccgca
     8221 cagagaactc tcccttacct gcagggctaa ttcctcaagt ttttcaatat cgccttcctg
     8281 tccttcgcct tcggtgatgc gggtgagaat ctccagcatt cgtttggtac caatgcggca
     8341 gaaggtgcac ttgccacacg attccttttg ggtgaaatca aggaagaacc ttgccacatc
     8401 aaccatgcag gtggtttcgt ccatcactac catgccccca gaacccatga tggctccggt
     8461 tgcggtaacc tgttcataat cgacctgaat gtcgataaga tgttcgggaa tacaaccgcc
     8521 cgaagggcct ccaagctgaa cggccttgaa ctttttatcg ttttgtatgc caccgccaag
     8581 cttgtagata atatcgcgca gcggcgttcc catgggtact tcaacaagtc caccgtgttt
     8641 tattttgcct gttagggcaa ataccttagt tcccttgctc ttttcggtgc catacttgtt
     8701 gtacgactcg gctccgtttg caacaatgta aggtacattg gcgtaggtct ccacgttgtt
     8761 gatattcgat ggtttttgcc aaagaccttt ctcagcaggg aaaggaggac gttttgatgg
     8821 catacccctt ttcccttcaa tggaagccat aagggctgtt tcctcgccgc aaacaaaagc
     8881 tccggcaccc tccttaatga agatatcgaa attgaaacct tggattccca tgatgttttt
     8941 gcccaggtag ccatgctcac gagcctgagc caaagcaata tttagccttt taatggccaa
     9001 tggatactct gctcggcagt atattacccc ctcggtggcg cctatagcgt atgcgccgat
     9061 tatcatccct tcaagcacag catgggggtc gccttcgagc agtgaacgat ccatgaatgc
     9121 gcctgggtcg ccctcatcgg cgttgcaaat gatgtacttc tcttccgact gattggcgcg
     9181 ggcaaagcgc catttcatgc cggttggaaa acctccacca ccacggccac gtaggcctga
     9241 gtcaataatg gtttgaatga tttgctcagg caaaagggct tcgttagcaa tctttcttaa
     9301 agcctgataa ccacctcgtg cctcgtattc gtggatattc tcggggtcaa tatagccaca
     9361 attcctcagc gcaatcttga cctggccgtc aaaatacgat tgatcagggg tggggaataa
     9421 atcggttttt accacatact ccttaatggg attgtgtccc aaaacgtgtt gctgtataat
     9481 ttcaatggcc ttgtcctcat caacatcgcc ataaatcatt gatccattct catctaccac
     9541 ctcaacaagt ggttcgcgaa aacacatgcc aatacagctg gtttttgcca gctcaaagtc
     9601 caaattatcg gcttccttta gagcttttaa cctattgtat accttgttgg ctccggctgc
     9661 aattccgcaa ctacctaagc caacaataac ttttgtttca gccattttta ttacgcgtta
     9721 tattgttttt aaaggttgat gtaacttgtg ggttactcac ctttagcttg agtttccttt
     9781 atcttaatgt ccttaactat tttcacagct gagctaccgg taagctttcc gtaggtttct
     9841 tcatcaatca tcattaccgg tgcaagcgaa cagcatccta agcaggctac tgtttcgagc
     9901 gtaaatagct tatcgggcgt ggtttcaccg tcgtttacct taagggcctc tttcagggct
     9961 gttgaaatgg cattggcgcc ctgaacgtgg catgcagtgc catgacaaac cttaatgata
    10021 tgcttaccaa ccggcgatag gcgaaattgt gcgtagaatg tagccactcc atacatatcg
    10081 ctcagggtta agccggtttc atgggcaatt agctcaaagg cagagcgggg aatatagccg
    10141 taaatggact gtaccccttg tagcaatggg attaaatttc cttttttccc cttatacttt
    10201 ttgataagtg gctcaatcag cgctaaatcg acttcatcgg caggaatgct acttgctggt
    10261 tttgtgagta tacgttgaac tcgcatggtt aagaatgatt agatattcaa tgttttaggt
    10321 tgatatgaaa acatattgat tactcaatac aaaaaaaaga cttttttggt ttgattgttc
    10381 tattcatggg caataaaagg ggatgttttt caaagcgaaa aacggagcac aaggcaatgg
    10441 gcctcatgct ccgtttgtta caaaactata agttagcgca aatagttttc atctcaaatc
    10501 atttaaacta caccctgagc aatcatggca tcggcaactt taacaaaacc accgatgtta
    10561 gcgccatcaa cgtagttgat aaatccatcc tctttggttc cgtatttacg gcatgtttca
    10621 tggatgttta tcatgatttg gtgcaggcgt tggtcaacct cctcgcgggt ccatgacaag
    10681 cgaagcgagt tttgcgacat ctcaagcccc gaggtggcta caccaccagc attggcggct
    10741 ttacccgggc cgtaaagaat tttggcgttt aggaaaacat ccacagcctc gggggttgaa
    10801 ggcatgttgg ctccttccga tacacagaag caaccgttac gaacaagcgt tcttgcctcc
    10861 tcgccgttaa tttcgttctg agtggccgat ggcaatgcaa tatcgcattt aataccccat
    10921 gggcgttctg ctgggaaata gtcgcagcca tacttttcgg catactcctt aatacgtccg
    10981 cgtttctcat tcttcagcca catcacaaat tttagttttt cgttgtcaat tcccttagga
    11041 tcgtagataa atccatcgga atcggaaagg gtaacaacct tgcctccaag ttcgttaacc
    11101 ttttcaacgg tatactgggc aacattgcct gaacccgaaa cggcaacaat tttatcctta
    11161 aagctttcgc cacgggtttt aagcatttcc tgggcaaagt aaacattgcc gtaaccggta
    11221 gcctcagggc gaatgagcga accgccccac tttaaaccct tgcctgtgag cacaccggta
    11281 aactcattta caatgcgctt gtactggcca aacaggtagc caatttcacg tccacctacg
    11341 ccgatatcac ctgcaggtac gtcggtgtcg gggccaatgt ggcggtatag ctcggtcata
    11401 aagctttggc agaaacgcat tacttccttg tcgctcttcc ccttggggtc gaaatcggag
    11461 ccacccttgc cgccacccat tggtaacgat gttaggctgt tcttgaaaac ctgctcaaat
    11521 gctaaaaact tcagaatact caaatttacc gtggggtgga aacgcaatcc gcccttgtag
    11581 gggccaatgg cgctgttcat ctcaatgcgg taaccccggt taacctgaat gttacccttg
    11641 tcgtcaatcc agggaaccct gaaaattact atgcgctctg gttcggtgat gcgctcaagg
    11701 attttttcat cctggtaaat tggattctca tcaatgtagg gcattatgct ctcaattacc
    11761 tcgcgggcag cctgatggaa ctcgggctca ttagggttgc gggcaattag gcctgccata
    11821 aagttgttca ctttttcctg taaagcaacg tttttcatat tgtattagtt tggttggtta
    11881 aacatgaaaa tctgaatgga aaattaagac cttttttaag cgatgtaaat agatgtgatt
    11941 taaaaacagc gaattacgga agtgtcttct acgtgaaaca cgtaggccga ttacgagtat
    12001 ttcttatttt gcctaaataa tttgacttta ttgcgtggta tacctaaatt acaggttgaa
    12061 tttgaaggat aaaggctaaa acgtgaatcg tgaaccgtga accgttaaac gggatagaac
    12121 gatatgcaat accacctgta tttacgaagg ataaaggttc gaggttaata gttcaaggtt
    12181 aaaggataaa gggcaaagga taaaggttaa aggataaagg ttaaaggata aaggttaaag
    12241 gataaaggtt aaaggataaa gataaaagtt ggatacgatg aataacagca agccaaatcc
    12301 cgaggag
//
LOCUS       DAOOWJ010000077        11868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5348_length_11868_cov_8.396597, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000077 DAOOWJ010000000
VERSION     DAOOWJ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11868
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5348_length_11868_cov_8.396597"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            768..1223
                     /locus_tag="PLW22_08255"
     CDS             768..1223
                     /locus_tag="PLW22_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74735.1"
                     /translation="MKRVLYVFGLLVISFGLKAQTDTIPNQQQNTAQTLMNSKPGLQI
                     GGYGEVHYNQPLKSNTHTLGTLDAHRMVLFFGYNFSSKTQFVTEVEIEYAREVWVEQM
                     FIQYRLNRYANFRAGVLLVPMGIINEYHEPTAFNGVERPIIDNKIAPTT"
     gene            1326..1973
                     /locus_tag="PLW22_08260"
     CDS             1326..1973
                     /locus_tag="PLW22_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74736.1"
                     /translation="MFSGSKGLREGRQKASKAYMNQPAFTGKLEFYGVKNLNVGLSGY
                     FGKSNSKLYSNLSKDSLAKIQRADSSVVGISMVGLDTRYNFKGVKLTGQLYYISLSNT
                     NEYNVFTRTGTKLNDLGSAMLGYYVEVGYDIFQTLADTKKSLMPFIRYEAYNTHHSVE
                     APVVTNKSYSNTIITTGLTYALEKGVVLKADLQFYKSKAENSFSKTFNAGIGVNF"
     gene            1983..2510
                     /locus_tag="PLW22_08265"
     CDS             1983..2510
                     /locus_tag="PLW22_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOU74737.1"
                     /translation="MKLLMAIFWVLLSLQVGSEINFEPKQISREVKRISGSQTPIAKE
                     IMAYKTGKFFTYTNANPVSFSYVGRVFTCRTEGCKASDDNVNANGSEYFDYFILFDRS
                     GKILSLQIYSFEATHGQEITLKSWLNQFVGYNGKNTLTVGKEIDAISGATTSVHRLTE
                     DVIDKTRKLQEILHQ"
     gene            complement(2579..3589)
                     /locus_tag="PLW22_08270"
     CDS             complement(2579..3589)
                     /locus_tag="PLW22_08270"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOU74738.1"
                     /translation="MGKITAAITGVGGYVPEYILTNEELSRMVDTTDEWIMTRIGIKE
                     RRILKGEGLGTSDMAVHAVNELLRKTNTHPDEVDMLICGVVTPDMQFPATANIISDKC
                     GIKNAFGYDMNAGCSSFLYALVTASKFVESGSHKKVVVVGADKMSSITDYTDRTTCPI
                     FGDGAAAVLLEPNHDGFGVIDAKMQVDGSGRKHLHQVAGGSCYPASFETVAKRQHFIY
                     QEGQPVFKAAVSNMADVSVEMMERNNIKPEDLAWLVPHQANMRIIEATANRMGIDKDK
                     VMINIQRYGNTTSATIPLCLWEWESKLRKGDNIIIATFGAGFTWGAAYVKWAYDGDKI
                     AK"
     gene            complement(3592..4536)
                     /locus_tag="PLW22_08275"
     CDS             complement(3592..4536)
                     /locus_tag="PLW22_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HOU74739.1"
                     /translation="MIRIGVDAMGGDFAPDAAVSGAVLALKHLNADERIVLFGDQQRI
                     LEILARENANPTDFDIVHTSEVIEMGDHPSKAFAKKTDSSIVKGFMALAAGKIDGFAS
                     AGSTGAMMVGAFMVIKQIPGVIRPGIAGFIPVSESKLNVILDVGLNPDCRPDVLYQYG
                     KLGSIYAQQVLGIDNPRVALLNIGEEEEKGNLVTKSAFEMMKDSTDYTFVGNVEGHHL
                     FHDKKADVVVCDGFVGNVVLKEAEAFYHLLKKFSPENKFIERFNSENYGGTPVLGVNK
                     PVIIAHGASSPVAIMNMIKQTREVIVSDLCEKIKQAFM"
     gene            complement(4564..4749)
                     /gene="rpmF"
                     /locus_tag="PLW22_08280"
     CDS             complement(4564..4749)
                     /gene="rpmF"
                     /locus_tag="PLW22_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOU74740.1"
                     /translation="MAHPKSRISKQRRNKRRTHYKAEAPTLSTCSNCGAAVEYHRVCP
                     ECGYYRGKLAIEKAVNA"
     gene            complement(4775..5305)
                     /locus_tag="PLW22_08285"
     CDS             complement(4775..5305)
                     /locus_tag="PLW22_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOU74741.1"
                     /translation="MASILGAYSISYKGLKLGKHSFDFEINNDFFAEFPEGEVKEGTL
                     TAKVMMNKQNNLLEFDIVIKGKVKVTCDRCLEQFFLPIEYKGFLVAKIGNEEVEDQAD
                     IIFLTEDDHEVNLAQYLYESIHLSLPLKRYHGLKGSSIEDCDQEMLKYIKFEDDDQVE
                     VDPRWQALKDLLNNNN"
     gene            complement(5393..7192)
                     /gene="typA"
                     /locus_tag="PLW22_08290"
     CDS             complement(5393..7192)
                     /gene="typA"
                     /locus_tag="PLW22_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOU74742.1"
                     /translation="MKGIRNIAIIAHVDHGKTTLVDKMIIQGKLFKDHENPGELILDS
                     NDLERERGITILAKNVSVNYKGYKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GTMPQTRFVLQKALAIGLKPILVINKVDKPNCRPEEVQEQVYELMFSLDASEEQLEFP
                     TIYGSAKQGWMSNDWRKPSNDITPLLDCIIDYVPAPKQLKGTPQLLITSLDYSNYVGR
                     IAIGKLHRGTLRAGQNVSLAKRNGQVMRTKIKELLVFEGLERKKVDEVSSGDICALVG
                     IEGFDIGDTIADYENPEPLPTIAVDEPTMSMLFTINNSPFFGRDGKYVTSRHLKDRLM
                     QELEKNLALRVEETDSADAFTVYGRGVLHLSVLIETMRREGYELQVGQPKVIIKEIDG
                     VKYEPIEYLTIDVPEDVSGKAIEQVTRRKGTLISMDRRHDRVHLEFEIPSRGIIGLRN
                     IILTATAGEAIMAHRFKAFEPYRGEIETRTNGSLIAMESGTAYAYALGKLQDRGRFFI
                     DEHEEVYTGQVVGENNRSGDIVVNVTKSKKLTNMRASGSDEKIFLAPPIKFSLEEALE
                     YIQEDEYVEITPKAIRLRKIYLDENERKRRAKA"
     gene            7293..8237
                     /locus_tag="PLW22_08295"
     CDS             7293..8237
                     /locus_tag="PLW22_08295"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOU74743.1"
                     /translation="MTKLLTTILLSILTITVNAQVTDAENQLKEIKTDSLSGWKRGLA
                     VGINANQTSLTNWAAGGQSSFALNTVFTGFVSYKSPKTAWDNTIDLGYGFLSQEGVKY
                     TKKTDDKIDLMSKFGREAFKNFYYAVLLNIKTQFTTGYKYPTDSTRLKISNFFAPAYL
                     VGALGMDYKPNTHLSVFAAPLTGKLTFVTDTMLSNAGAFGVKKGETLREEIGGYVRVV
                     YTKNDFNAEWLKNLTFTTKLDLFSNYLDNPQNIVVNWETLVIMKVNKYINVNLATQLI
                     YDDKVKISKDNNGDGVIDSSGPRVQFKEIFGVGITFKL"
     gene            8292..9818
                     /locus_tag="PLW22_08300"
     CDS             8292..9818
                     /locus_tag="PLW22_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOU74744.1"
                     /translation="MPLLNSILNWVNTKRLYSIDLFRKYPFEVQRDVLFELLKQGSKT
                     EYGARYGFDGIRTIEEFQKRVPLVDYETIKPYIERLRQGEEKLLWPTDIKWFAKSSGT
                     TQSKSKFIPVSNEALEDCHFRGARDVLAIYMNLYPDNNLLSGKGLTLGGSHQVDNFNM
                     NSFYGDLSAILIENQPWWAEFIRTPSQKVALIPDWEEKLEKLTHETLNENVTSLAGVP
                     SWNLVMIKHLLSYTGKSNLLEIWPNLELFMHGGVSFAPYREQFQKVIPSPNMHYMETY
                     NASEGFFAIQDDPASDSMLLMLDYGIFYEFVPLDELTKPNPTALTIADVEVGRNYAMV
                     ITTNSGLWRYLIGDTVTFTSKHPHKIRITGRTRHFINVFGEEVIVENAERALEKACKA
                     TGALVAEYTAAPVYMGTDRKGCHEWFIEFVKQPSSIEEFARVLDSTLCEVNSDYEAKR
                     AKNVTLDFPVVTQAVQGTFFEWMRQRGKLGGQNKVPRLSNTREYIDELLAIHNQLKSK
                     "
     gene            9959..10573
                     /locus_tag="PLW22_08305"
     CDS             9959..10573
                     /locus_tag="PLW22_08305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU74745.1"
                     /translation="MHIAIAGNIGSGKTTLTGLLAKHYKWKPQYEDVDENPYLNDFYE
                     DMQRWSFNLQIYFLNSRFSQIVEIHRSGHDVIQDRTIYEDAHIFAPNLHDMGLMSTRD
                     FNNYLSLFNLMTSLVKPPDLLIYLRATVPTLVRQIQKRGRDYEKNIRLDYLQRLNERY
                     EAWIEGYKLGKLLIIDVDNLNFPDRPEDLRFIINKIDAQIHGLF"
     gene            complement(10670..11866)
                     /gene="kbl"
                     /locus_tag="PLW22_08310"
     CDS             complement(10670..11866)
                     /gene="kbl"
                     /locus_tag="PLW22_08310"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOU74746.1"
                     /translation="MYGKIKNDLQQQLENIKAEGLYKTERIITTPQGVEIVANGKKVL
                     NFCANNYLGLSSHPAVIEAGKKAIDEWGFGMSSVRFICGTQDLHKQLERKLSEFLGTE
                     DTILYSSAFDANGGIFEPILGEEDAIISDELNHASIIDGVRLCKAQRFRYKNNDMADL
                     EAKLQEAANCRYKMIVTDGVFSMDGIIAQVDKICDLADKYDAMVMVDDSHATGFVGKT
                     GRGTAEYCNALGRVDVISTTFGKALGGASGGCISGRKEIIDMLRQRSRPYLFSNSLMP
                     SIAGATLKVLNMLTETTELRDRLWENTQLFRKGMTEAGFRIVEGVHPITPIMFGHLPN
                     DAKLSQDFAAALLEEGIYVIGFYYPVVPKGKARIRVQISAAHTREHIQFAIEKFTKVG
                     KKLGVI"
BASE COUNT         3292 a         2690 c         2538 g         3348 t
ORIGIN      
        1 cgtgactcat gtggtatcgc ttactaagcc caccgccacg ttccaccccc gacccattgc
       61 cgggtcaggc ttttatgcct ctgcttgctt tttccgctcc attccctctc agggaaaagg
      121 gctggggtga ggtcctatcc aatgaacaac gaacaactta caagaatgac ttcatttgtc
      181 atcctgagta cagcgaagga tctctaatcc ttcccatgag attttcggct tagttcaaca
      241 tgacaataat ttcacgaaaa acgcttcaga cccgtaagga ctaaatgttt atgtaccaat
      301 gacctttccc tcatgtattg cgtgcatcgc ctaataattt gaaaaccaca ataaccaggg
      361 ttaaaaccct gagcttagct gatttgcccc tactgggctg gaaacggcgt tcgttgttcg
      421 tgtatcgttg ttcgtgattg ccactctgtg taacaacgtg ttgcactgtg taaccctgtg
      481 ataccaattt agttgatagt tgtttgttga tggttgatgg cttaagaaac caccttccat
      541 catgaatctg aaatctgaaa tctgaaattt taaattttta tcctttaacc ttaaaaaaca
      601 aacaccacca ctctccacac ttagaatatt gctctttaac aagttcagga tgttattgga
      661 gtcacaattc taaatagagt tgattgataa acatcaatac tatttttgca taaaatagaa
      721 ttagtctaaa taaatttgcg gcgaaaacaa ttaacaataa tgccgttatg aaaagagttt
      781 tgtatgtatt tggtttactt gtgatttcgt ttggtcttaa ggcccaaacc gacaccattc
      841 ccaatcagca gcaaaatact gctcaaacac taatgaacag taaaccaggg cttcaaattg
      901 ggggatatgg tgaggttcac tacaatcaac cgttaaagag caacacccac accctaggta
      961 cgctcgatgc tcatcgtatg gtgctgttct ttggttacaa tttttccagt aaaacgcagt
     1021 ttgtaaccga ggttgaaatt gagtatgcca gggaggtttg ggttgagcaa atgtttattc
     1081 aatacaggtt aaacaggtat gcaaacttta gagctggagt actgcttgtg ccaatgggta
     1141 ttattaatga gtaccatgaa ccaaccgctt tcaatggtgt tgaacgtccg attatcgaca
     1201 acaaaattgc gcccacaacc tagcgcgagg tagggctagg cttccaagga aatgtttctc
     1261 aggccaaaac caggtatcag ctctatgtag taaacggatt aaatggttac gatggaacca
     1321 atgctatgtt ttccggtagt aagggactta gggagggccg tcaaaaagcc tcaaaggctt
     1381 acatgaacca acctgcattt acaggtaaac tggagtttta tggggttaaa aatcttaatg
     1441 ttggattatc gggctatttt ggaaaatcga atagcaaact ttactcgaac ctcagtaagg
     1501 atagcctcgc aaaaatccaa agggccgact catcggttgt tggaatatca atggttggtt
     1561 tagatactcg ttacaatttc aaaggcgtta agcttactgg ccaactttac tacatttcac
     1621 tttctaatac caatgagtat aatgtattta ccaggactgg taccaagctt aacgatttag
     1681 gaagtgctat gctcggttat tacgttgagg ttgggtatga catatttcaa acgttggctg
     1741 acactaagaa aagcttaatg ccctttattc gttatgaggc ttacaacact caccattcag
     1801 ttgaggcacc agttgtaaca aataaaagtt atagtaatac tatcatcacc acaggcttga
     1861 cctacgcttt agaaaaagga gtagtactta aggcagattt acagttttat aaatccaaag
     1921 ccgaaaactc ctttagcaag acatttaatg cgggtatagg agtgaatttt taatcttgct
     1981 gtatgaaact gttgatggca attttttggg ttttattaag cttgcaggtt ggatctgaaa
     2041 tcaactttga acccaaacag attagcaggg aggttaagag aataagcggg agtcaaacgc
     2101 ccattgccaa ggagattatg gcctataaaa caggtaaatt cttcacttat accaatgcta
     2161 acccggtttc attttcctat gtaggaaggg tatttacatg cagaaccgaa ggttgcaagg
     2221 cttccgatga taatgttaat gccaatgggt ctgagtactt tgattacttt atccttttcg
     2281 acaggtcggg taaaattctc tcacttcaga tttatagctt tgaggccact cacggccagg
     2341 aaatcaccct aaagagttgg ttaaatcaat ttgttggcta caacggaaaa aatacgctga
     2401 cagtaggcaa agaaattgat gcaatatcag gcgctaccac ttcggttcat cgcttaacag
     2461 aagatgtaat tgataaaacc cgaaaacttc aggaaatact gcatcagtaa tgttagactc
     2521 taacaccaaa taataaaaaa gccgggctaa acccggcttt tttgttgaaa acaatatttt
     2581 actttgcaat tttatcacca tcgtacgccc actttacata ggctgcaccc caggtaaaac
     2641 ctgctccaaa ggttgcaata attatattat cgcccttgcg aagtttcgat tcccattccc
     2701 aaaggcatag agggattgtg gcactggttg tattgccgta gcgctgtatg ttaatcatca
     2761 ccttatcctt gtcaattccc atgcgatttg cagttgcttc aattattcgc atgtttgcct
     2821 ggtgtggaac aagccaagcc aaatcctcag gcttgatatt gttgcgctcc atcatctcaa
     2881 ccgacacatc ggccatgttt gaaacagcag ctttgaacac gggttggcct tcctggtaaa
     2941 tgaagtgctg gcgcttggct acagtttcga acgaagcagg gtagcatgaa ccaccggcca
     3001 cctggtgaag gtgtttgcgt cctgagccat caacctgcat cttggcgtct attacgccaa
     3061 agccatcatg gttaggttct aggagcacag ctgctgctcc gtcgccaaaa atgggacagg
     3121 tggtgcggtc agtgtaatcg gttatggacg acatcttgtc ggcgccaacc accacaacct
     3181 ttttatgtga acccgactcc acaaactttg atgctgtaac caaagcatac aggaaacttg
     3241 aacagcctgc attcatatcg taaccaaaag cattcttaat cccgcactta tcgcttataa
     3301 tgttagcggt agccgggaac tgcatgtcgg gggtaactac gccgcaaatt aacatgtcca
     3361 cctcatcggg gtgggtgttg gttttacgga gcagctcgtt aacggcatga actgccatat
     3421 cggaagttcc aagcccttcg ccctttagta ttcggcgctc cttaatacca atacgggtca
     3481 ttatccactc atcggtggta tcgaccattc tacttaactc ctcgttggtg aggatgtact
     3541 ctggcacata tcctccaacg ccagtaattg cggcagtaat ttttcccata gctacataaa
     3601 tgcttgttta attttttcgc aaaggtccga tactataacc tcgcgggttt gtttaatcat
     3661 attcattata gccacggggc tcgatgctcc atgggcaatt ataacgggtt tattaacccc
     3721 aagaactggc gtgccaccgt agttctcgga gttaaaacgc tcaatgaact tattttcagg
     3781 gctaaatttc ttaagcaggt ggtagaacgc ctcagcctcc ttgagtacta cgttacccac
     3841 aaacccatcg caaactacca catcggcctt tttatcgtgg aataggtgat ggccctcaac
     3901 gtttccaaca aaggtataat cggttgagtc cttcatcatc tcaaaggcgc ttttggttac
     3961 caggttgcct ttctcctcct cctcgccaat attcagtagg gcaacccttg ggttatcaat
     4021 gccaagcact tgctgggcgt aaattgaacc cagtttacca tactggtaaa gtacgtcggg
     4081 acggcaatcg gggtttaaac ccacatcgag aataacatta agcttacttt cggaaacagg
     4141 gataaaaccg gctattccgg gacgtataac accgggtatt tgctttatca ccatgaaagc
     4201 gccaaccatc atggctccag tgctgccagc actggcaaaa ccatcaattt tgccagcagc
     4261 caaagccata aagcctttaa ctatactgga atcggttttc ttggcaaacg ctttcgatgg
     4321 gtgatcgccc atttcaatca cctcagaggt gtgaactata tcaaaatcgg taggatttgc
     4381 attttcacgg gcaaggattt caagaatcct ttgctggtca ccgaaaagca caatgcgctc
     4441 atcggcatta agatgcttga gggctaaaac tgcacccgac acagcagcat cgggtgcaaa
     4501 atcgccgccc attgcatcaa cgccaattct aatcatcaat gcaggggttt atgaaattca
     4561 tttttaggcg ttaactgcct tctcgattgc taacttacca cggtaatagc cgcactcagg
     4621 gcaaaccctg tgatattcaa ctgctgctcc gcagtttgaa catgttgaaa gggttggagc
     4681 ttcggccttg tagtgagttc tacgcttgtt ccttctctgt ttagagattc gtgatttcgg
     4741 atgtgccatt tttcaacaaa ttatatagtc caacttagtt attattgttc aacaaatcct
     4801 ttaaagcctg ccagcgaggg tcaacctcaa cctggtcatc gtcttcaaac ttgatgtact
     4861 ttagcatttc ctggtcgcaa tcctcaatac tggatccttt taacccatgg taccgcttta
     4921 gcggtagact caggtgaatg ctctcgtaca ggtactgggc aaggtttacc tcatggtcat
     4981 cctcggttag gaagattata tcggcttgat cctcaacctc ctcgtttcca atttttgcca
     5041 caagaaaacc cttgtactca attggcagga agaattgttc caagcaccta tcgcaggtaa
     5101 cctttacctt tccttttatc actatgtcaa attcaagcag gttattctgc ttgttcatca
     5161 taacttttgc tgtgagcgtg ccttccttaa cctcaccctc agggaactca gcaaaaaaat
     5221 cgttgtttat ttcaaaatca aacgagtgtt tgccaagctt aagtcccttg tagctaattg
     5281 aatatgcgcc taaaatgctt gccactttct cacaaaatca acgtgcaaag ttataaaaaa
     5341 tcaattcaac ccaaaaaaag ttaaaaatat tcatcaaaaa agtaccgttc cgctatgctt
     5401 tagccctacg tttacgctcg ttttcgtcga ggtatatttt tcgtaggcgt atggctttag
     5461 gggtgatctc aacgtattcg tcctcctgga tgtactcaag tgcttcctca aggctaaact
     5521 tgatgggggg agcaaggaaa atcttttcgt cggaacctga ggcgcgcatg ttggtaagtt
     5581 ttttggactt tgtaacatta acaacaatat cgcctgaacg gttattctcg ccaaccactt
     5641 gacctgtgta aacctcctca tgttcatcga taaagaacct acccctgtcc tgtagcttgc
     5701 ccagggcgta agcataagcg gtaccgctct ccatggcaat tagtgaacca ttggtgcggg
     5761 tttcaatctc gccccggtag ggctcaaatg ccttaaagcg gtgcgccatg atggcttcgc
     5821 cggcagtagc cgttagaatg atgttacgga gcccaataat accgcgcgag ggtatctcga
     5881 actcaagatg aacacggtcg tggcggcgat ccattgagat tagtgtaccc ttgcggcggg
     5941 taacctgctc aatagccttt cccgatacat cctcaggaac atcaatggtt aggtactcaa
     6001 tgggttcgta ctttactcca tctatctcct taataattac cttgggctga ccaacctgaa
     6061 gctcataccc ttcgcgacgc atggtttcaa taagcacgga aaggtggagt actccacgac
     6121 cgtaaacggt aaaggcatcg gccgaatcgg tttcttcaac ccgaagggct aggttttttt
     6181 ctagctcctg cattagccta tcctttaggt gacgcgaggt aacgtatttc ccatcgcgac
     6241 caaagaacgg agagttatta atggtgaaga gcatgctcat ggtaggctca tccacggcaa
     6301 tggtaggaag tggctcgggg ttttcgtaat cggctatggt atcgccaatg tcaaaccctt
     6361 caatgccaac aagggcacag atatctcctg agcttacctc gtcaaccttt ttgcgttcaa
     6421 gaccttcaaa aacgagtagt tccttgatct ttgtacgcat tacttgtccg tttcgctttg
     6481 ccaacgatac gttttggcct gcccttaggg ttcccctgtg cagcttgcct atggcaatcc
     6541 ggccaacata gtttgagtag tcaagcgatg taataagaag ttggggcgtg ccttttagct
     6601 gttttggagc tggcacatag tcaataatac aatcaagcag aggggttata tcgtttgatg
     6661 gcttccgcca gtcgttgctc atccatccct gtttggcgct tccgtatatg gtagggaact
     6721 cgagctgttc ctctgaggca tcgaggctga acattagttc gtaaacttgc tcctgaacct
     6781 cctcagggcg acagtttggc ttatcaacct tgttaatcac cagaattggt tttaagccta
     6841 tggctaaagc tttttgaaga acaaaacggg tttggggcat agtcccctca aaggcatcaa
     6901 ccagaagcag cacaccatcg gccatgttta gaacgcgttc aacctcgcct ccaaaatcgc
     6961 tgtggccggg ggtgtcaatt atgttgattt tgtaaccctt gtagtttacc gatacgtttt
     7021 tggccagaat ggttatacca cgctcacgct ccaggtcgtt gctatccaaa atcagttcgc
     7081 ccgggttctc atgatcttta aaaagtttac cctgaatgat cattttatcc accagcgtgg
     7141 ttttcccatg atccacatgc gcaataattg caatgtttct gatacccttc atccccaaaa
     7201 aattagcccg caaagttaca gctttgaaat gaatattttc ccataataat aattagtatt
     7261 ttagcgtaaa ttaatgaata attcatgcag ccatgactaa attacttacc actattttgt
     7321 tatcgatact tactataact gtaaacgctc aggtaaccga cgctgaaaat cagcttaaag
     7381 agattaaaac cgattcgctt tccggatgga agagagggct ggccgtggga attaatgcca
     7441 accagacttc gttaaccaat tgggcggccg gcggacaaag ctcatttgca ctaaataccg
     7501 tttttacagg gtttgttagc tataaaagtc ccaaaactgc ttgggataac actattgact
     7561 tgggctacgg atttctgagc caggagggag ttaagtacac caaaaaaacc gacgataaaa
     7621 ttgacttaat gtcgaaattt ggacgtgagg ccttcaaaaa cttttactat gctgttttgc
     7681 tgaacattaa aacccaattc actaccggtt ataaataccc aaccgatagt acccgtctga
     7741 aaatttcgaa cttttttgct ccggcatacc tggttggggc attgggtatg gattataaac
     7801 cgaacaccca cctgagtgtt tttgcagcac cgttaaccgg aaagctaacc tttgttacgg
     7861 acaccatgct atcgaatgca ggagcatttg gcgtaaaaaa aggggaaaca ctcagagagg
     7921 aaattggcgg ttacgtgaga gtggtttaca ccaagaatga ctttaacgcc gaatggctca
     7981 aaaacctcac ctttacaact aagctcgacc tattctccaa ctaccttgat aacccacaaa
     8041 acattgtggt taactgggaa acgcttgtaa ttatgaaggt taacaagtac atcaatgtta
     8101 acctggcaac ccagctaatc tacgatgaca aggttaagat tagtaaggat aacaacggcg
     8161 atggtgtaat tgatagcagt gggccacgtg ttcagtttaa ggagattttt ggcgtgggta
     8221 ttaccttcaa gctttagccc ttttgatttt tgggcaaaat gacttagttt tgagttgata
     8281 attttttgat tatgccttta ctcaactcta tcctgaactg ggtaaacact aagcgcttat
     8341 acagcatcga tcttttccgg aaataccctt ttgaggtgca acgcgatgtg ctttttgagc
     8401 ttttaaaaca aggctcaaaa acggaatacg gagcacgata tggctttgat ggcatcagaa
     8461 ccattgaaga atttcagaag cgagtacccc ttgttgacta cgaaaccatt aagccataca
     8521 ttgaacggtt gaggcagggc gaggaaaagt tgctttggcc aaccgatatt aagtggtttg
     8581 ccaagtcatc gggaactacc cagagcaaga gtaagtttat tccagttagc aacgaggcgt
     8641 tggaggattg ccatttcagg ggggctcgcg atgtgcttgc catttacatg aacctttatc
     8701 cggacaataa ccttttaagc ggcaaaggac ttaccctagg tggtagccac caggttgata
     8761 actttaatat gaattccttt tacggcgatt tatcggctat tctgattgaa aatcaaccct
     8821 ggtgggctga gtttatcaga accccaagcc aaaaagtggc attaataccc gattgggaag
     8881 agaaactgga gaaactcact cacgagaccc ttaacgagaa tgttaccagc ctggcaggag
     8941 tcccttcatg gaatctggta atgattaagc acctgctgag ctacacaggc aaaagtaact
     9001 tgcttgagat atggccaaac cttgagcttt tcatgcatgg cggagtaagt tttgccccat
     9061 acagggagca gtttcaaaaa gttatcccat cgcctaacat gcactacatg gaaacatata
     9121 atgccagcga gggtttcttt gccattcagg acgaccctgc gtcggatagc atgttgctta
     9181 tgctcgacta cggtattttt tatgagtttg ttcctctcga tgagcttacc aagcctaatc
     9241 ctacagccct taccattgcc gatgttgagg ttggaaggaa ttatgccatg gtcattacca
     9301 ccaattctgg cctttggcgt tacctaattg gcgatacggt tacctttacc tcaaaacacc
     9361 ctcataaaat caggattaca ggcagaaccc gtcacttcat caatgttttt ggcgaagagg
     9421 ttattgttga aaacgctgag cgtgcacttg aaaaagcctg taaggctacc ggagcattgg
     9481 ttgctgagta caccgctgct cccgtttaca tgggaactga ccgtaaggga tgccatgaat
     9541 ggttcattga gtttgtaaaa cagccaagca gcatcgagga gtttgctcga gttcttgact
     9601 caacgctttg tgaggtcaat tccgactatg aggcaaaacg cgccaaaaat gtaacgctcg
     9661 attttccagt ggttacgcag gctgttcagg ggacattctt tgagtggatg cgccagcgcg
     9721 gtaaattggg tgggcagaat aaagttcctc ggctttcgaa tactcgcgag tatatcgatg
     9781 agctgcttgc cattcataac cagctaaaaa gtaagtagtc atcaaaatag ataggattta
     9841 tttacatttt tgacacattc atcaatctac aagctgtttt accttgccga ttaaaatcca
     9901 atttgtatct ttagaaacga aaacgtgaat taccaacctt aagtaattat tttaagttat
     9961 gcatatagcc attgcgggta atattggttc tggcaaaaca acccttacgg gtttactggc
    10021 caagcactac aagtggaaac cccagtacga ggatgttgat gaaaacccat accttaacga
    10081 cttttacgag gatatgcagc gctggagctt caacctccag atttacttcc taaacagcag
    10141 gtttagtcaa atagtggaga ttcaccgaag cggtcacgat gttattcagg atagaaccat
    10201 ttacgaggat gcccatattt ttgctccaaa cctccacgac atgggattga tgagcacccg
    10261 cgattttaac aactaccttt ccctgttcaa ccttatgacc tcgcttgtta aaccgcccga
    10321 tttgctaatt taccttcgtg ccacagtgcc aacgctggta aggcaaatcc agaaacgcgg
    10381 acgcgattac gaaaaaaata tccgcctcga ttacctccag cgccttaatg aacgttacga
    10441 ggcatggatt gaaggctaca agttaggcaa gctgctaata attgatgttg acaatctgaa
    10501 tttccccgat agacccgaag acctcaggtt tattatcaat aagatcgatg cgcaaataca
    10561 tggtttgttt tagataagaa aagggagtca aaaaaagact cccttcaatt tttatctgtg
    10621 agattgacag gttgataaag gtatcaatct ttctgatagg atgatttctt taaatcacac
    10681 ccaatttctt gcccaccttt gtgaactttt caatggcaaa ctggatatgt tcgcgggtgt
    10741 gagctgcact tatctgaacc cttatgcggg ctttaccttt aggtacaacg gggtaataga
    10801 aaccaatcac ataaataccc tcttcaagca aggctgcagc aaagtcctgc gatagcttag
    10861 catcgttggg taggtggcca aacattattg gggtaatggg gtgaacaccc tcaactatac
    10921 ggaaaccggc ttcggtcatt cctttacgga atagctgagt gttttcccaa agcctatcgc
    10981 gtagttcggt agtttcggta agcatgttaa gaactttaag tgttgctccg gcaatggaag
    11041 gcatgagcga gtttgaaaaa agatagggac gcgaacgctg acggagcatg tcgataatct
    11101 ccttacgacc agagatgcaa ccacccgatg cacctccaag tgcttttcca aacgtggttg
    11161 aaataacatc gaccctgccc agtgcattgc agtactcagc agtaccacga ccggttttgc
    11221 ccacaaaacc tgtagcatgt gaatcgtcca ccattaccat ggcgtcgtac ttatctgcta
    11281 ggtcgcatat cttgtcaacc tgagcaataa tcccatccat tgagaataca ccatcggtaa
    11341 caatcatttt gtaacggcag ttggcggcct cttgaagttt ggcctccagg tcggccatat
    11401 cgttattttt gtagcggaat cgctgagctt tgcaaagcct aacaccatca atgattgatg
    11461 catggttaag ttcgtcggaa ataattgcgt cctcctcgcc tagtatgggc tcaaaaatac
    11521 caccgtttgc atcgaatgcc gatgagtaaa gaattgtatc ctcggtccca agaaactcgc
    11581 ttagcttacg ctcaagctgc ttgtgtaggt cctgcgttcc gcaaataaag cgcaccgacg
    11641 acatgccaaa gccccactca tcaatagcct ttttgcctgc ctcaataact gcaggatgcg
    11701 acgagaggcc aaggtagtta ttggcgcaga agtttaaaac ctttttgccg ttggcaacta
    11761 tttcaactcc ttgcggggta gtgataatgc gttcagtttt gtaaagccct tcggccttga
    11821 tattctcaag ctgttgctgt aaatcgtttt tgatttttcc gtacatag
//
LOCUS       DAOOWJ010000078        11850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5359_length_11850_cov_8.391691, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000078 DAOOWJ010000000
VERSION     DAOOWJ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_11850_cov_8.391691"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..2834)
                     /locus_tag="PLW22_08315"
     CDS             complement(189..2834)
                     /locus_tag="PLW22_08315"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOU74747.1"
                     /translation="MKHNLTACSMLMAAMITFSFNTLLAQEMSKNTCTALHVNPHPPI
                     IDGALNDEVWQKDNWCSGFTQHEPHNGAEPSQQTEFMITYDNDYLYAAFRLHDTEPDK
                     IVARVTRRDDIEGDFVAIGIDSYFDKRTAFIFCVSASGTKMDMVMTENGDREDPNWDP
                     IWWAKTARNPNGWTAEMKIPLNQLRFSAADVQTWGIQVGRHLYRNQETDFWQHVPKDA
                     PGFVHLFGELHGLKGLTPKRQVEVAPYVVTKTESFKPEEGNPFMTGRRNGLNAGVDAK
                     IGLTNNFTLDLTVNPDFGQVEADPAVINLTEFETYFEEKRPFFIEGSNLLNFPLMFGD
                     GDLATNNLFYSRRIGRAPQHYPDLGDNEYARVPSNSTILGAAKLTGRSSSGLSVGLLQ
                     SVTGNAWANIDSAGNRSHEMVEPLTNYTVVSAKQEYNEGNTIVSGMVTSTNRALSGIM
                     ADEYHRNAFSGGVDLMHLWANKKYMVEAKLYGSHVNGSKDAILLTQTNSSHYFQRPDI
                     KHVKIDSSRTSLTGTGGVLQFGKVGEGHIRYMAAVAWRSPQLEINDIGYVQNVDDIFQ
                     VIWVGLRYWEPTKVYRSLNVNINQWTGWNFGGESTYKGGNINLNLQLPNYWYVGGGIN
                     WNGENLSVSELRGGPALKLPGGWNYWLSLNSDGRKKLIVGVSNSAYIGNYNSSVSHNY
                     SLSLTYKPLKSLLLRVSPSYSFSKSELSYVDNISTVSDTRYVRGHLNRNTWVMSLRVE
                     YSINPELSVQYYGRPYFTSGKYSRFKYITQPRAQKYTDRFLTYTDSQISYDADNEVYL
                     VDENTDGTTDYSFDNRNFNYLNLQSNLIIRWEYRPGSTVYFVWTHGKENFESVYSNSI
                     GNDIDNLWDSHPHNIFLVKFSYRFN"
     assembly_gap    3287..3386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3387..3784)
                     /locus_tag="PLW22_08320"
     CDS             complement(<3387..3784)
                     /locus_tag="PLW22_08320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOU74748.1"
                     /translation="MVAIKNPYEGLPEHGCFCCSSKNPIGLKLSFWYDETNQTVETRW
                     LPELNYQGYHGVLHGGIQSTLMDEVASWCIYILEGTAGVTHSMEIVYSKPMLIGKGQV
                     VVRGRIDNREKRLVTVHVELFDGESNLCTTG"
     gene            3898..4509
                     /locus_tag="PLW22_08325"
     CDS             3898..4509
                     /locus_tag="PLW22_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU74749.1"
                     /translation="MDTKHGNATRLKLMETARELFIAKGVDRVGVREIASKAGVNLSL
                     MNYYFQSKENLLEQIFEQSIKDNGQKIRQILNADMPLAEKIKVYINTYIDILTEDPLL
                     VPFVLSIIHRNAEQASRLKAVHTLYNTETFSNQLKHEAELGNIRKVDPEQFFVSMISL
                     ILFPFAIKWLIGYRMTLSNEQMAQFLEDRREHVYDLLMSALRP"
     gene            complement(4541..4831)
                     /locus_tag="PLW22_08330"
     CDS             complement(4541..4831)
                     /locus_tag="PLW22_08330"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOU74750.1"
                     /translation="MSDHNTKSLGEAIKAFIDAMKWNDNFNQASIRSDWERIVGRDIA
                     MHTTDIQFKEGILTLSLNSAALRQELHMRRKDLLTHINEYFGSEVVKEIRVK"
     gene            complement(4834..5943)
                     /locus_tag="PLW22_08335"
     CDS             complement(4834..5943)
                     /locus_tag="PLW22_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOU74751.1"
                     /translation="MYLKRLTAINFKSINQLDIEFDNKINCFVGNNGQGKTNLLDAIY
                     FLSMCKSSLSSTDLQVIRHGQDFFVLQGFYSRQGVDESIYCGLKQGEGKIFKRNGKAY
                     ERLADHIGFLPVVMVSPADNELVEGSGEERRKYMNGVISQIDHIYLDEVLRYNRLLAQ
                     RNSLLKTYSRTAFSYDLIEAIDIQLAEYASVIHSKRKAFVEQLIGILQEYYNKVSGGQ
                     EKVSLSYKSHLNDTPAETLFRENIEKDRVLQYTSVGIHRDDLTLLIDGYPIRNEGSQG
                     QQKTYLIALKLAQFDFISKVAGIKPLLLLDDIFDKLDTGRVHRLIDMVANDGFGQIFI
                     TDTNKNRLEEVLAKVTSGYRFFRVDNSEFELVESR"
     gene            6065..6757
                     /locus_tag="PLW22_08340"
     CDS             6065..6757
                     /locus_tag="PLW22_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74752.1"
                     /translation="MADKKKQQTTDTVESIDNALTRTEQFIEQNQKSLTIIVAAILAI
                     VAIYFGYKKLYLQPKENEASSQIFQAQYYFEQDSFRLALYGNENDLGFATIADRYGMT
                     RTGNLANYYAGICLRELGEYQKAIDYLKKFDAGDQMVTPIAYGAIGDCYVELNKLKDA
                     AKFYIKAANYEKNDFTTPIFLKKAGMVYEELKQYDKALDLYNIIKNDFTKSIEARDIE
                     KDIARINTLMGK"
     gene            6840..7343
                     /gene="ribH"
                     /locus_tag="PLW22_08345"
     CDS             6840..7343
                     /gene="ribH"
                     /locus_tag="PLW22_08345"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOU74753.1"
                     /translation="MATYLKNLSSYDPNMVPSAKNMKFGIVVSDWNDSVTHKLLEGAY
                     SALIEHGATDENILVKHVPGAFELTLGGQFMAEYADLDAVICLGCVIQGETRHFDFIC
                     QGVTYGITELNMNYNIPFIFGVLTTDNLEQALDRAGGKHGNKGVEAAITAIKMAALQK
                     EMEELEG"
     gene            7674..10706
                     /locus_tag="PLW22_08350"
     CDS             7674..10706
                     /locus_tag="PLW22_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU74754.1"
                     /translation="MKRVFWVFFPLIFAQTISAQQSNPLISKADSASQMRWIDSVFNS
                     LSLRERIAQLFMVAAYSNKDDSHVMEISQLVSKQGIGGLIFFQGGPVRQARQTNYYQS
                     LAKVPLLIGIDAEWGLGMRLDSTISYPRQMALGATNDTLLTYQMAADIAHQFKRMGIH
                     INFAPVIDINSNPLNPVIGSRAFGQDMEMVTRHGMAYTKGLQENGILACAKHFPGHGD
                     TYDDSHLTLPLVPHSANRIDSIELYPFKKLFAAGVASVMVAHLKIPSLEPDTLLPASL
                     SARIVGQKLFGELGFNGLAFTDAMNMKGVADFYNPVDANIRSLMAGNDILLFPSEIEN
                     TIKKVEQLIKSEQIPEELINIKCKKVLMAKYLVGLNKYRPINVDRLYNDLNSPYSQVL
                     RRKIIRNAITVIHNANNILPLRGLDTLRIAYIEIGENKGDAFREQLELYTAVHTFSIN
                     PEAEQAEYDTLLTNLEPYNLIIVGYHTIESKAAKGFGVSQQSSNFLFDLSFRKKVILN
                     LFGNPYSISRFANPLAFAAIIVSYDNSTDSQNLTAQTIFGGIPFSGRLSVTASDLFGI
                     NTGYASNQRIRLGYSIPEELGIGSEQLSEVDSLAMEIIRTGAAPGMQILAAKDGVVFY
                     NKAFGKPTYVSEYGNDIRMLYDIASVTKVAATLPPIMNLYNRGVLSFDSKLGQFIDLR
                     NFPDKSKLLLGDILRHQSGLQAWIPFYMNTLTSLEPNIPLWTETFSQTHPYKYNGKRY
                     ASRFAYPSPKFYRTDSISNFPNNVAYRIYSLASINDSIYRWINQSPISDAGKYRYSDL
                     GFLYLQKVIETVEQKRLDKVVEPLYSKLGMNYTCFNPLLRFDTERIAPTEHDPVFRKQ
                     LIWGHVHDPAAAMLGGVAGHAGLFANANDLAKLMQLYLNDGEYGGERFFAPSTVKSFA
                     NPATTNGNRRAYGFDKPNASGSSPAGTMASPESFGHTGFTGTMVWADPQNGLVFIFLS
                     NRVHPDAENTKLSAMNYRTRIHDVFYRAIQSISKPSGQ"
BASE COUNT         3415 a         2668 c         2383 g         3284 t
ORIGIN      
        1 atgtgtgtcg cttaaatcta tttctaacct gtagacttat ttcaggacta gacacaggaa
       61 tgagtcggaa tattgggaat gggaggaagt tagaggaagt tagaagaaaa gagaacagtt
      121 aaaccgacgt gggataaaat aaaaaaagcc ggatacaaat tccggctata cattgcggta
      181 tctttgcttt aattaaatcg atacgaaaac ttaaccaaga atatgttgtg cgggtgcgag
      241 tcccaaaggt tatcaatatc gttgccaatg gaattgctgt aaaccgattc aaaattttct
      301 ttcccatggg tccatacaaa gtaaactgtg gaaccggggc ggtattccca ccgaataatc
      361 aggtttgact gtaggttcaa gtaattgaaa ttcctattgt caaatgagta atcggttgtg
      421 ccatcggtgt tttcatctac cagatatacc tcattgtcgg cgtcataact tatctgtgaa
      481 tcggtatatg tcaaaaacct atcggtatac ttttgggccc tgggctgagt aatatacttg
      541 aatcgggaat acttaccgct tgtaaaatat ggcctaccat agtattgaac tgacagttca
      601 gggttaatgc tatactccac ccgtaacgac atcacccagg tgttcctatt cagatgtccc
      661 ctaacatacc ttgtatcact cacagtactt atgttatcca cataggaaag ttcactttta
      721 ctgaacgagt acgatgggct aacccttagc agtaaacttt ttagaggttt gtatgtcaat
      781 gacaatgagt aattatggct tacagagcta ttgtaattgc ctatataggc actattgctt
      841 acaccaacaa ttaatttctt ccgtccatcg gagttaaggc taagccagta gttccatccc
      901 ccgggtaatt ttagagcagg gccgcctcta agttccgata cactaaggtt ttcgccattc
      961 cagttaatac ctcccccaac ataccagtag ttgggcagct gaaggttcag gtttatgttt
     1021 ccgcccttat aggttgattc gccaccaaaa ttccagcctg tccattggtt aatattcaca
     1081 ttcaacgacc tgtaaacctt agtaggttcc cagtatcgca acccaaccca gataacctgg
     1141 aatatatcat caacattttg aacataacca atatcattta tttccagctg aggcgaacgc
     1201 caagcaacag cagccatgta gcgtatatgt ccctcgccca ctttgccgaa ttgaagcaca
     1261 cccccagttc cggtaagtga cgttctggat gagtctatct tcacatgttt aatatcgggg
     1321 cgttgaaaat agtgggatga gttggtttgg gttagcagaa tagcatcctt gcttccatta
     1381 acgtgactcc cgtatagctt ggcttcaacc atgtactttt tatttgccca taaatgcatt
     1441 aaatccactc cccccgagaa agcattacgg tggtactcat cggccattat tcccgatagt
     1501 gctctattgg ttgaggtaac cattcccgaa actattgtat taccctcatt gtattcctgt
     1561 tttgcggaaa caacggtata gtttgtgagc ggttcaacca tttcatggct gcggttgcca
     1621 gcagaatcga tatttgccca tgcattacct gtaacacttt gcagcagacc aaccgataaa
     1681 ccactacttg atcggccggt tagcttggca gccccaagaa tagttgagtt tgaaggtacc
     1741 cgtgcgtatt cgttatcgcc caaatcgggg taatgctggg gtgctctacc aattcttcgg
     1801 gagtaaaata gattgtttgt agccaggtcg ccatcgccaa acatcaacgg aaagttaagc
     1861 aggttgctcc cctcaatgaa aaagggacgt ttctcctcaa agtaggtctc aaattcagta
     1921 agatttatca cagcgggatc ggcctcaact tgaccaaaat cgggatttac agttaaatca
     1981 agggtaaagt tattggttaa accaattttg gcatcaactc cggcattcaa accattgcgc
     2041 cttccggtca taaaagggtt cccctcttcg ggtttaaaac tctctgtttt ggttacaaca
     2101 tacggtgcaa cctcaacctg gcgcttgggt gttaagcctt tcaggccatg tagctcgcca
     2161 aaaagatgca caaaaccagg cgcatcctta ggaacatgct gccagaaatc ggtttcctgg
     2221 ttcctgtaaa ggtgacgccc cacctgaata ccccaggttt gaacatcggc tgctgagaat
     2281 cggagttggt tcagggggat tttcatttcg gcagtccaac cgttcgggtt tcgtgcagtc
     2341 ttagcccacc aaataggatc ccagttaggg tcctccctat cgccattctc ggtcattacc
     2401 atgtccatct ttgtacctga agctgaaacg cagaaaataa atgcggttcg cttgtcaaag
     2461 taggagtcaa taccaattgc aacaaaatcg ccctcaatat catcgcgacg ggtaacgcgt
     2521 gctacaattt tatcgggctc tgtatcgtgc aaccggaaag cagcataaag gtaatcgtta
     2581 tcgtacgtaa tcataaactc cgtttgctgc gatggttcag caccattatg gggttcatgc
     2641 tgggtaaatc cactacacca attatctttt tgccaaacct catcgttcaa tgccccatca
     2701 attatagggg gatggggatt aacatgtaac gctgtgcatg tattttttga catttcctgg
     2761 gccaacaatg tattgaatga aaaagtaatc attgctgcca tcaacattga gcatgcggta
     2821 aggttatgct tcataggtaa aatagttatt ttaaccaaat gacgcatata tgacgcctaa
     2881 tgctgctcaa aggctacttt agaagtgtta ataattgcta aaagcgtgtg gacgttggct
     2941 ttgcaaaaga gttacgttga gtatagttga tagttgacgg ttggtagttg atggttggta
     3001 gttgatggtt gatggttgat agttgacagt atactgaaat actattctgg aaagatagta
     3061 aacacttaat gccaaaacgt ttaacggttt acggctaacg gtttatggct aacggttctc
     3121 gaacctttac tattttgtca ctactcacct ccgtccaaaa atgcatctct gccgggatat
     3181 ccatactttg acttggcgat ttcggttgga aaaacaaagt attttatttt accggtagtg
     3241 caaaggttgc tttctccatc aaaaagttca acatgcacgg ttaccannnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnccgg tagtgcaaag gttgctttct ccatcaaaaa
     3421 gttcaacatg cacggttacc aaacgttttt cacgattatc aattcggcct cgaactacca
     3481 cttggccttt accaattagc atcggcttac tgtaaacaat ctccatgctg tgggtaactc
     3541 ccgctgtacc ttcaagtatg tatatgcacc agcttgctac ctcgtccatt agggtcgatt
     3601 gaatgccacc atgcaaaaca ccgtggtaac cctgataatt tagctcaggt aaccaacggg
     3661 tttcaacggt ttgattagtt tcatcgtacc aaaagctgag ctttaaacca ataggattct
     3721 tactggagca gcaaaaacag ccgtgctctg gcaaaccctc gtatggattt tttattgcaa
     3781 ccatttcaat tcaattattt accttgacaa ttttaaaaca attttaaaaa acttaaacct
     3841 aaaacatatg tttattttaa acagatttta ataattttga gaaaaactta aaagagtatg
     3901 gataccaaac acggaaacgc aacccgttta aagttgatgg aaacagctcg ggagttgttt
     3961 attgcaaaag gagtcgatag agttggcgta cgtgaaattg catccaaagc cggcgttaat
     4021 ctatcgctca tgaactacta ctttcagagt aaggagaact tacttgagca gatctttgag
     4081 caatccatca aggataatgg acaaaaaatc aggcaaatac ttaatgccga catgccactt
     4141 gcagaaaaga ttaaggttta tatcaatact tacatcgata tattaactga agatcccttg
     4201 cttgttccct ttgtactttc aattattcat aggaatgccg aacaggcatc aagactaaaa
     4261 gcggttcata ccctttacaa caccgaaact ttttcaaacc agcttaaaca cgaggctgag
     4321 ctgggcaata ttcgaaaggt tgacccggaa cagttctttg ttagcatgat ctcgttaatc
     4381 ctcttcccgt ttgccattaa gtggctaatt ggttatagaa tgacattgag caatgagcaa
     4441 atggctcaat tccttgagga taggcgcgaa catgtttacg acttgctgat gagtgcactc
     4501 cgcccttagt acaacaaacc ccacaaaggc tgtggggttt ttacttcacc ctaatctctt
     4561 ttaccacttc gctcccaaag tactcattga tatgggtaag caaatccttc ctgcgcatat
     4621 gcagttcctg ccttagagcg gctgaattta ggcttagggt caatatccct tccttaaatt
     4681 ggatatcggt tgtatgcatg gctatatcgc ggcctacaat tctttcccaa tcggaacgga
     4741 ttgatgcctg attaaagtta tcgttccact tcatggcatc aatgaaagcc tttatggctt
     4801 cgcccaacga ttttgtgttg tgatcgctca tgattacctc gactcaacca gttcaaactc
     4861 agaattatca accctaaaaa agcgataccc cgacgttacc tttgccaaaa cctcctcgag
     4921 cctattcttg ttagtatcag taataaatat ttggccaaat ccatcatttg ccaccatatc
     4981 aattaaccta tgcaccctgc cggtatcgag cttgtcaaaa atatcgtcga gcagcaaaag
     5041 gggtttaatg cctgcaacct tagaaatgaa atcgaattgt gcaagcttaa gtgcaatcag
     5101 gtaagttttt tgttgcccct gagagccctc gttgcgaatg gggtatccat caattaaaag
     5161 cgtaaggtcg tcgcggtgaa ttccaaccga ggtgtactgc aaaaccctgt ccttttcgat
     5221 attctcccga aaaagtgtct cggcaggggt atcgttcagg tgcgacttgt agctcaacga
     5281 aaccttttcc tgtccgcccg acaccttgtt atagtactcc tggaggattc caataagttg
     5341 ctccacaaac gccttgcgtt ttgagtggat taccgaagca tactccgcta actgtatgtc
     5401 aatagcctca atcaaatcgt aactaaaagc ggtacgggaa taggttttta acaggctgtt
     5461 gcgttgagca agcaatcggt tatagcgcaa aacctcatcg aggtaaatat ggtcaatttg
     5521 tgaaataact ccattcatat acttgcggcg ctcctcgcca cttccctcaa ccagctcatt
     5581 atcggcaggc gaaaccataa ccaccggaag aaaaccaatg tggtcggcca gcctttcgta
     5641 ggctttaccg ttacgcttaa atattttccc ttcgccctgc tttaacccgc aatatatgct
     5701 ctcatcaaca ccttggcgcg agtaaaaacc ctgcagtaca aaaaaatcct gtccgtgacg
     5761 tatcacctgt aaatcggttg acgaaagcga actcttgcac atggaaagga agtatattgc
     5821 atcgagcagg tttgtcttac cctgaccatt gttccccaca aagcagttta tcttattatc
     5881 aaactcgata tcaagctgat ttatcgactt aaagtttatg gctgttagcc ttttaagata
     5941 catagtagct aaaaatgaat atttgcaaag gtaattaaac cgaaaatgtt tgcctaaaat
     6001 tttcagatat tacaaaaaca attacttttg cgattgcttt gttaaaaata aatattcatt
     6061 agttatggca gataaaaaga aacagcagac aaccgatact gtagaatcaa ttgacaacgc
     6121 tttaacccgt actgagcagt tcattgagca aaaccagaag agccttacca ttattgtagc
     6181 agcaatttta gctattgtgg ctatctattt tggttacaaa aaactttact tacagcccaa
     6241 ggagaacgag gctagctctc aaattttcca ggctcagtat tactttgagc aggattcgtt
     6301 tcgcctggca ctatatggta acgagaacga tttaggcttt gccactatag ctgataggta
     6361 cggaatgacc cgcaccggga acctggcaaa ttactatgca ggcatctgcc ttagggaact
     6421 tggtgaatac caaaaggcca ttgattacct aaaaaagttt gatgccggcg accaaatggt
     6481 taccccaatt gcttatggcg ctattggcga ttgctatgtg gaactcaaca aactaaagga
     6541 tgctgccaag ttttatatta aggccgccaa ttacgagaag aatgatttta ccacgccaat
     6601 tttcctgaaa aaggctggaa tggtttacga ggaactcaaa cagtacgata aggcccttga
     6661 cctttacaat atcatcaaga atgattttac aaagagcatt gaggctcgcg atattgaaaa
     6721 agatattgcg cgtataaaca cactgatggg aaagtaaaga ctaaacagag ttatatttaa
     6781 ggggtgcttt taaacacccc ttattttttt acaaattcgt atctttgaaa aaaaatccta
     6841 tggcaacata tctgaaaaac ctatcgagct atgaccctaa catggtgcct agcgcaaaga
     6901 acatgaagtt tggcattgtg gtttcggatt ggaacgattc tgtaacccat aagctacttg
     6961 agggtgccta tagcgcccta attgagcatg gcgccaccga cgaaaacatt cttgtaaagc
     7021 acgttccggg tgcctttgag ttaacccttg gcggacagtt catggccgag tatgccgatt
     7081 tggatgctgt tatatgcctt ggctgtgtta ttcagggcga aacccgccat ttcgatttta
     7141 tttgccaggg tgtaacatac ggaattaccg aactcaacat gaactataat attcctttca
     7201 tttttggagt gctaactacc gataaccttg aacaagcact cgaccgcgct ggagggaaac
     7261 acggcaacaa gggagttgaa gcagcaatca ctgccattaa aatggctgcg ctccaaaaag
     7321 aaatggagga actggaagga tagcggattc gttgttcgtt gatggttgtt cgttgatggt
     7381 tgttcgttga tggttgttcg ttgctcgtga tatttattac ctgatatttg acctaaggtt
     7441 tggcattata cgaaataagt gtaaaaaatt tgtatccccc ctttaacggt taacggttca
     7501 cgtttaacgg ataacggtta acgattcacg gctcacggtt cacggttcac gaacctcttt
     7561 taacttttca cttttaactc cactcccacc cttctcaaca aaatcagctt aaaaaatggc
     7621 acaatttcca gctaatgcta tatttgcatt accggagcct taaccaatta aaaatgaaac
     7681 gtgttttttg ggtatttttt cctttgatat ttgctcaaac aattagcgca cagcaaagca
     7741 acccattgat aagcaaagct gattcagcca gccaaatgcg ctggatcgat tccgttttca
     7801 acagcctaag cttaagggaa cgtattgcac agctttttat ggtggctgca tactccaaca
     7861 aggacgattc ccatgtaatg gagatttcgc aacttgtatc aaaacaagga attggtgggt
     7921 tgatattctt tcagggtgga cctgtccgtc aagcacggca aactaattac taccaatcgc
     7981 ttgcaaaggt tccccttctt attggcatcg acgccgaatg gggattagga atgcggttgg
     8041 atagcaccat ctcgtatccc aggcaaatgg cgcttggtgc caccaacgat accttgctaa
     8101 cctaccaaat ggcagccgat attgcccatc agttcaaacg aatgggtatc catatcaact
     8161 ttgcaccggt tatcgatatt aactccaatc cgctcaaccc cgttattggc tccagagctt
     8221 ttggtcagga catggaaatg gtaaccaggc atggcatggc ttacacaaaa ggactacagg
     8281 aaaatggtat tctggcttgt gctaagcatt ttcccggcca tggtgacacc tacgacgatt
     8341 cacacctaac cctacccctg gtgccacata gcgcaaaccg aatcgattcc atagaactct
     8401 accctttcaa aaagcttttt gctgctggtg tagccagtgt tatggttgca cacctaaaaa
     8461 ttccctccct tgaacccgat accctcttgc ccgccagcct atcggcccgc attgttggcc
     8521 aaaaactttt tggcgaactt gggtttaatg gccttgcatt taccgatgcc atgaatatga
     8581 aaggtgttgc tgatttctac aacccggttg atgccaacat acgttccctt atggccggca
     8641 acgacatact gctctttcca agcgagattg aaaatactat taaaaaagtt gaacagctta
     8701 ttaaaagcga acaaatcccc gaagaactta tcaacattaa atgcaaaaag gttcttatgg
     8761 ccaagtatct ggttggttta aataaatacc gcccaattaa tgttgatagg ctttataacg
     8821 atttaaacag tccttactcg caggtactcc gccggaaaat tatacgaaac gcaattactg
     8881 taatacacaa tgcaaataat atccttcccc ttagagggct cgatactctt aggattgctt
     8941 atattgaaat tggcgaaaat aaaggcgatg cattccggga gcagcttgag ctttacactg
     9001 ccgtgcatac tttctccatc aaccccgaag ccgagcaggc agagtacgat accctactta
     9061 ccaatctaga gccatacaac ctaattattg ttggttacca cactattgaa agcaaggctg
     9121 caaagggttt tggggtaagt caacaatcat caaatttcct gttcgaccta tcgttccgta
     9181 aaaaggtgat tcttaatctg tttggcaacc cctactccat tagcagattt gctaatcctt
     9241 tagcatttgc tgccataatt gtatcgtacg ataactcaac cgatagccag aaccttacag
     9301 cccaaaccat ttttggtggt attccgttct caggcaggtt aagcgtaacg gcaagcgacc
     9361 tgtttggcat taacaccggt tacgcatcaa accaaagaat tagacttgga tacagcatcc
     9421 ccgaggaact tggaataggg tcggaacagc tctcggaagt tgactccctt gcaatggaaa
     9481 taataagaac aggggctgct ccaggcatgc aaatccttgc agcaaaggat ggggttgtat
     9541 tctataacaa ggcctttggg aaacccacct acgtttcgga gtatggcaac gatatcagaa
     9601 tgctttacga tattgcatcg gttaccaagg ttgccgcaac gttgccacct attatgaacc
     9661 tgtataaccg aggtgtgctg tcgttcgatt caaaacttgg ccagtttatc gatttaagaa
     9721 acttccccga taagtcgaaa ctacttttag gtgatatctt gaggcatcaa tcgggtttac
     9781 aggcctggat tccattttat atgaacacat taacatcgtt ggaacctaac ataccgttgt
     9841 ggactgaaac attcagccaa actcatccct ataaatataa cgggaaacga tacgccagcc
     9901 ggtttgccta cccaagccct aagttctacc gaaccgattc aatcagcaac ttcccaaata
     9961 atgttgcata ccgcatatac tctttggcaa gtatcaacga ttccatttac cggtggatta
    10021 accagtcgcc aatttccgat gcgggtaaat accgatatag cgatttgggt ttcctatacc
    10081 tgcaaaaggt aattgaaaca gttgaacaga aaagattaga caaggtagtt gaaccgctgt
    10141 atagcaagtt agggatgaac tacacctgtt ttaatccgct actacgtttc gacaccgaaa
    10201 gaatagcccc aacagaacat gaccccgttt tccgtaaaca gctgatatgg gggcatgtac
    10261 acgatccggc tgctgccatg cttggcggag ttgctggtca tgcaggcctt tttgccaacg
    10321 ccaacgattt ggctaagctt atgcagctgt acctgaacga cggagaatac ggtggcgaac
    10381 gcttttttgc tccctcaacg gtaaaaagtt ttgccaaccc agctacaaca aatggaaacc
    10441 gaagggctta cggcttcgat aagcccaatg ccagcggttc gtcgcctgcg ggaacaatgg
    10501 cttcgcccga aagctttggc cacacaggtt ttacaggaac aatggtatgg gctgacccac
    10561 aaaacggatt ggtatttatt ttcctatcca accgtgttca ccccgatgct gaaaatacta
    10621 aactttcagc aatgaattac cgcacacgaa ttcacgatgt attttaccgt gccattcaaa
    10681 gcattagcaa gcctagtggg caataattga gaagaggcac aacaactgca ttccacaagg
    10741 tgtagaaaat tttctacacc tttttgcttt ttatattgga attcgtttca aactcaaatt
    10801 atataaacct gattctcttc tagttgtgat tttttcttaa ctattaaaat ctattcaata
    10861 attctggcac ggttttgtta ttacttatga cgtcccaaac accataaata cataaattgc
    10921 tgttgccacc agcagccagg ttgtccggat caccgcattt actgtcggcc atgcatcgaa
    10981 tccaccatta gtcccagatc taaaagacag tataacccgg acaacttttt tttatccaag
    11041 attcaatgtc gttaagatta caaaaatata catgcctgtc atcctgagcg tagcgaagga
    11101 tcactaatcg accccatgag acgtttcgct tagttcaaca tggcaatagt ttcacgaaca
    11161 acaaacaacg cttccagccc cgtaggggcg aaacatttgt aaccccatgg ctactcccac
    11221 atgtatagcg atgcacgaaa tgcctttacc aaagagcaaa ggtgaaactt tgcatcgcaa
    11281 aagaaaaatc attacaaaga attatttaca cgttctttct tgtcttgata caagaaagaa
    11341 ccaaagaaaa tcaaggctaa aaagcccgac ccgatgggta ccccgcgggc ggaactgcca
    11401 cgcgatacta ttcgccacgc ctgcggcgtg actcatgtgg tatcgcttac taagcccacc
    11461 gccacgttcc acccccgacc cattgccggg tcaggctttt atgcctctgc ttatgtcatt
    11521 ctgtgttggc tgaggaatct cattaatata tagggatgaa atcacaaaga gatgcttcga
    11581 cttggctcag catgacaaag ggagtgaggg agccaatggt attgtcattc cgagctagtc
    11641 gtggaatctt ttatgtcctt accgcttcca gccctgtagg ggcgaaacat ttgtaaccca
    11701 tggctactcc cacatgtata gcgatgcacg aaatgccttt accaaagagc aaaggtgaaa
    11761 ctttgcatcg caaaagaaaa atcattacaa agaattattt acacgttctt tcttgtcttg
    11821 atacaagaaa gaaccaaaga aaatcaaggc
//
LOCUS       DAOOWJ010000079        11776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5408_length_11776_cov_7.773483, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000079 DAOOWJ010000000
VERSION     DAOOWJ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5408_length_11776_cov_7.773483"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1095)
                     /gene="tsf"
                     /locus_tag="PLW22_08355"
     CDS             complement(268..1095)
                     /gene="tsf"
                     /locus_tag="PLW22_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOU74755.1"
                     /translation="MAEITAADVAKLRKLTGAGMMDCKNALVEANGDMERAIELIRER
                     GKAIANKRADREAGEGAALSKVNQAGSRGAMIVLNCETDFVAKNADFLGVANKILDHA
                     LNTAPANLDALKESSLDGRKVADFVAEFSGITGEKVELSYYEQVEAPMVVSYIHMGNK
                     LATLVGFSKAIDIQAAKDVAMQVAAMSPVAIDKDDVPEDIRQKEFEIGREQARLEGKP
                     ENMLDKIAEGKLKKFYKESTLLNQEFVKDNKLTIRQYLQSVDKDVKVTAFKRVSLNL"
     gene            complement(1126..1995)
                     /gene="rpsB"
                     /locus_tag="PLW22_08360"
     CDS             complement(1126..1995)
                     /gene="rpsB"
                     /locus_tag="PLW22_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOU74756.1"
                     /translation="MSRTNFQELLEAGVHFGHLKRKWNPKMAPYIFMEKNGIHIIDLH
                     KTVVKIDEAAAAVKQIAKSGRKILFVATKKQAKDIVAERIKAINMPYVTERWPGGMLT
                     NFPTIRKAVKKMNSIDKMMTDGTFDTLSKREKLQIQRQRAKLEKNLGSIANLNRLPAA
                     LFVVDVQKEYIAVREANRLNIPVIAMVDTCCDPTPIDFVIPANDDASKSIALIVDIMA
                     KAIQEGLEERKAEKDKEPAAAEKEEGVKTRARKAKKAADVVEEPEDEDEADEVEEVEK
                     PTATIVDDEIDEE"
     gene            complement(2115..2501)
                     /gene="rpsI"
                     /locus_tag="PLW22_08365"
     CDS             complement(2115..2501)
                     /gene="rpsI"
                     /locus_tag="PLW22_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOU74757.1"
                     /translation="MQVYNGLGRRKSAVARVYLREGKGNITINGRDLKEYFSSELLHY
                     VVKQPLMVLNATEGFDIKATLDGGGITGQAEALRLGIARALVEYNEANRAIMRANGFL
                     TRDPREVERKKPGRPKARKRFQFSKR"
     gene            complement(2510..2965)
                     /gene="rplM"
                     /locus_tag="PLW22_08370"
     CDS             complement(2510..2965)
                     /gene="rplM"
                     /locus_tag="PLW22_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOU74758.1"
                     /translation="MNTQSYKTVMANDATVKKEWVVIDATDQVLGRLSSQVAKILRGK
                     HKPSYTPHVDCGDNVIIINADKVRLTGRKLTDKEYVRHTGYPGGQRFSTPKEMLHRKP
                     IWVIEHAVKGMLPKNRLGAEMFRNLYVYAGSEHPHEAQQPKKIELSNIK"
     gene            complement(4549..5100)
                     /gene="def"
                     /locus_tag="PLW22_08375"
     CDS             complement(4549..5100)
                     /gene="def"
                     /locus_tag="PLW22_08375"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOU74759.1"
                     /translation="MIRTVYVYGSPVLRKVAQNITPDYPQLDALIADMFETMEQSDGI
                     GLAAPQIGLSIRLFVVDGTPLADDDPTLKDFRKAFINAQITERFGEKVIYNEGCLSLP
                     GLHEDVERESKVRIRYHDEKFVEHEEVYEGMAARIIQHEYDHLDGILFTDRVSPIRKQ
                     LLRSKLNAIAKGKYSASYKCRIG"
     gene            complement(5126..5545)
                     /gene="ruvX"
                     /locus_tag="PLW22_08380"
     CDS             complement(5126..5545)
                     /gene="ruvX"
                     /locus_tag="PLW22_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOU74760.1"
                     /translation="MGRIVALDIGRKRTGIAMTDPLRIIASPLETIPTHKVIDYLTEL
                     FLKENIDILVVGNPRQMNNTPSEAVNYIKPVINAIKKRFPEMPIELVDERFTSKMALQ
                     AMIDGGMKKKDRQEKSNVDKISAAIILQSYMEGLKYK"
     gene            complement(5555..6307)
                     /locus_tag="PLW22_08385"
     CDS             complement(5555..6307)
                     /locus_tag="PLW22_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU74761.1"
                     /translation="MLKALIVDDEIASLRTLELLLGHFEQHIEIIGVAYSASEGIDKV
                     TKYDPDIVFLDIEMPGGSGFDFLEGCPHRNFDVIFITAYNNYAIKAFKYSAIDFLLKP
                     IEVDELHEALEKVIRRRYSNFDGRNKYFALFENIKEIIPQKLVVSTNDKYECLDVGEI
                     VFVESVPNGTQIKLVNGSHFLANNPFGDICTLLEERNFSFINPSCLINLGMIRKISNK
                     PESNIELKNHELITVRGEYLERLNHQLENISK"
     gene            complement(6427..7776)
                     /locus_tag="PLW22_08390"
     CDS             complement(6427..7776)
                     /locus_tag="PLW22_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU74762.1"
                     /translation="MPTNRERIEEWSKAYWLLRFYQNFLFKLYFKTEIVGMEKLPPNT
                     TFIFAPNHQNALIDALAMLTLKHSWQPIFLARADIFKKPLVSKILTFLKIMPVYRIRD
                     GFENLSLNDSIFLKTMDIIRKGNGLVILPEGNHAGFKILRPLKKGIARIALQTEDASD
                     GSLNIHIVPVGLDYSNYVRYRSKLLIRIGEPFPVKKYLDLYRQNKALAYNALIDELSE
                     RIKAEMIHIEDEQNYYEYLFLTQLFPKRFIKENKLPNTLNQAFEISKRLILKLDRLKE
                     TDRSTFDEIIQLAHVLRDRISANRIKPQALPLKVSKAFNLLWMVPLFVVLSPLAIFGF
                     INHIIPIIIPYQLSKKFEDVQFHSSVRHAAGLLLLPLAYLIQTVIVGLILKSLAMALL
                     YLVLLPPAAVILYYWRKLFYKIRGLTSILIFAMRNRNEFELINNHFNRLMNDIKSIF"
     gene            complement(7807..9183)
                     /locus_tag="PLW22_08395"
     CDS             complement(7807..9183)
                     /locus_tag="PLW22_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOU74763.1"
                     /translation="MKRVSTITQKLLVFLLVILLSSLANAETIAKKIKTIPQGSDSAF
                     VTKTLGSPTEKQVVSRYFYQGNQAVFIDSVIRDIRLAEPHKKLKIKLKRQLKAEQKKE
                     TPISSLRIGMNISEALERAGEPDSIVKGEDWYYTNRHRIEIANGKVIKTEVHLKATLE
                     TLDWIWLNFTKGGLLFMNITLALIMFGIALQIKIEHFKLLLVSPKPVILGFLSQFLAL
                     PLFTFLLVLIIQPTPSVAMGMILVAACPGGNISNFISSLAKGNVALSITLTAIATLAA
                     IFMTPFNFAFWGKLYSAASNLVIPIEIDAWEMIRTVLILLGIPVILGIAFARFFPNTA
                     ERISKPFKIFSIVAFLGFVVAALAANFGYFLKYIHLIALIVIVHNGLGLLVGYTIPSI
                     FKLSKQDRRTIAIETGIQNSGLGLVLIFNPNLFDGLGGMAFIAAFWGIWHIVSGLGIA
                     YYWSRKPL"
     gene            9372..9893
                     /locus_tag="PLW22_08400"
     CDS             9372..9893
                     /locus_tag="PLW22_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOU74764.1"
                     /translation="MPVKIRLSRQGRKKRPFYHIVVADSRAPRDGRFIERIGSYNPLT
                     NPATIELNFDRALDWLQKGAQPTDTCRAILSYKGVMLKKHLLEGVKKGALTPEQAEAK
                     FQDWVSQKEAKIKAKIDSLAKGEAEEMKKRMEAESKVRDARAQEIAKKRTEMAAKEAD
                     SQAESTSEETENA"
     gene            9905..10426
                     /gene="rimM"
                     /locus_tag="PLW22_08405"
     CDS             9905..10426
                     /gene="rimM"
                     /locus_tag="PLW22_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOU74765.1"
                     /translation="MGDAGFVEIGYIQRTHGINGELAVSLNSSVEFNPEELESVFLEI
                     EGIPVPFFITRIRFQNPEKAIVKFDDVDSIDQAQELYGVKMLIPSTSIELEDEVYLSD
                     LVGYKVRNTDKSEVGVIVDYTEYSMNATFELVTPDGKHVLIPAADELIVEVDTSAKLL
                     EMELPEGLIDLNL"
     gene            complement(10421..10861)
                     /locus_tag="PLW22_08410"
     CDS             complement(10421..10861)
                     /locus_tag="PLW22_08410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU74766.1"
                     /translation="MSSNPKLHKFFHRTPIQIRFNDIDQLSHVTNSVYQQYFDLGRLK
                     YFTEVLQEKMNWEIEGLVLASITIDYLIPIRMWDNIEVLSKVYEIGNKSLKMRQEIFN
                     NTTGEVAAVSKAVMVCFSNAAGKSIPIPHRWRELILSFEKDLQL"
     gene            complement(10836..>11776)
                     /locus_tag="PLW22_08415"
     CDS             complement(10836..>11776)
                     /locus_tag="PLW22_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOU74767.1"
                     /translation="YELELELKETFQFFDFETVIGSVTNEYRVNRLFEAFKPQVVFHA
                     AAYKHVPMMENNPTEAVFNNVLGTKLVADIAVKFHVEKFVMISTDKAVNPTNVMGASK
                     RIAEIYTQTLNYTSQTKFITTRFGNVLGSNGSVIPRFKKQIENGGPITITHPDIIRYF
                     MTIPEACQLVLEAGAMGQGGEIFIFDMGKPVKILDLAKKMIKLSGLTYGKDIQISFTG
                     LRPGEKLYEELLNNKENTIPTYHPKIMIAKVNEYKQEEVMPKIMELLNTLPSHNNFRI
                     VSIMKDIVPEFVSRNSIFEEIDQQKKYRNEQQSKTA"
BASE COUNT         3169 a         2463 c         2613 g         3531 t
ORIGIN      
        1 ccttatatga gatccctcga ctacgctcgg gatgacaaca tcattggctc cctcattcac
       61 tttgtcatgt tgagtcccga aatatcggga tagactccag cgaaacatct catggggtag
      121 attagagatc ctttgctccc cactagggcg ggacaggccg cgcgggacga caggcatgta
      181 tatttttgtt aactaaacaa cattgagtta gatataataa aaaaggagag caaccctgct
      241 ctccttcaag gtgatatgct gtaaggatta taggttgagc gatacgcgtt tgaatgcggt
      301 taccttaacg tccttatcaa cgctttgcag gtactggcgt atggttaact tgttatcctt
      361 aacaaattcc tggttgagaa gggtgctctc cttgtagaat ttctttagct tgccttcggc
      421 aatcttatcg agcatgtttt cgggtttgcc ctcaaggcgt gcctgctcgc ggccaatttc
      481 aaactccttc tggcgaatat cttcgggaac atcgtccttg tcaatagcca cagggctcat
      541 ggcggcaacc tgcatggcaa catccttagc ggcctggata tcgattgctt tgctgaagcc
      601 taccagagta gccagcttgt tgcccatgtg gatgtatgaa accaccatgg gtgcttcaac
      661 ctgctcgtag tacgatagtt caaccttttc gccggtaata ccggagaact cggcaacaaa
      721 atcggctacc ttgcggccat cgaggctcga ttccttaagc gcgtcgaggt tggcaggtgc
      781 tgtattcagg gcatggtcaa gtattttatt ggctacgcca aggaaatcgg cattttttgc
      841 aacaaagtcg gtttcgcagt tgagcacaat catggcaccg cgggaaccgg cctggttaac
      901 ctttgaaagg gcagcgcctt cgccggcttc acggtcggca cgtttgttgg caatggcctt
      961 accgcgttcg cggataagtt caatggcgcg ttccatatcg ccgttagctt caaccagagc
     1021 attcttgcaa tccatcatgc ctgctccggt aagtttacga agttttgcta catcagcagc
     1081 tgtaatttca gccatggtat ttagtttttt ttagggtcta agttgttatt cttcgtcaat
     1141 ctcatcgtca acgatagtgg cagtgggttt ttcaacctct tcaacctcgt cggcttcatc
     1201 ctcatcttcg ggctcttcaa ctacatcggc agccttttta gccttgcgag cgcgggtttt
     1261 tacgccctct tctttttcgg cagcggcagg ttccttatct ttttcagcct tacgttcctc
     1321 aagcccttcc tgaatggctt tagccatgat gtcaacaatc agggcgatag atttggatgc
     1381 atcgtcgtta gcgggtatca caaaatcgat tggagtagga tcacagcagg tatcaaccat
     1441 tgcgataacc ggaatgttaa gcctgttggc ttcacgaacg gcgatgtatt ccttttgaac
     1501 gtcaaccaca aaaagggctg ctggtaagcg gttgaggttg gcaattgaac caaggttctt
     1561 ttccagcttg gcgcgttggc gttgaatttg gagcttttca cgctttgaaa gggtgtcgaa
     1621 agtgccgtcg gtcatcatct tatcgatgct gttcatcttt ttaacggctt tgcggatggt
     1681 ggggaagttg gtgagcatac caccaggcca acgctcagta acgtaaggca tgttaatagc
     1741 cttgatacgt tcggcaacaa tgtcctttgc ctgttttttg gtggcaacaa aaaggatctt
     1801 acgacccgat ttggcgattt gctttactgc agcggcagct tcatcgattt ttactactgt
     1861 tttgtgcagg tcgataatgt ggataccgtt tttctccata aagatgtaag gagccatctt
     1921 tgggttccac ttacgcttta agtgaccaaa gtgcacacct gcttcgagca gttcctgaaa
     1981 atttgttctt gacatttttt gtttgtttag attttttggc aatcgctcag tagtcccttt
     2041 cgggagtctg ggcaatttat tactaaactg cctgccaaat ggttaataaa tagtttgtta
     2101 aacaattgat agcattaacg tttgctgaac tggaatctct ttctggcttt tggcctaccg
     2161 ggtttcttac gttcaacctc acgaggatcg cgggttaaga aaccgttggc gcgcataata
     2221 gccctgtttg cctcgttgta ctcaacaagt gcgcgggcaa taccaagcct aagagcctca
     2281 gcctgaccgg ttataccacc accatcgaga gttgccttaa tgtcaaaacc ttcggtggcg
     2341 ttaagtacca ttagaggctg ctttaccacg tagtggagca gctctgagct gaaatactcc
     2401 ttgaggtcgc ggccatttat ggtgatgttg cctttacctt cgcggaggta aacacgggca
     2461 acagccgatt ttcttctacc taatccgtta taaacctgca taagttaact tatttaatgt
     2521 tgcttaattc aattttcttt ggttgttgtg cctcgtgcgg atgctcggaa ccagcataaa
     2581 cgtaaaggtt gcggaacatc tcggcgccaa gcctgttctt aggcagcatg ccctttacag
     2641 cgtgttcaat aacccaaatg ggcttgcgat gaagcatttc cttaggggtt gagaaacgct
     2701 gaccaccagg gtatccggtg tggcgtacat actccttatc ggttaactta cgaccggtta
     2761 aacgaacctt gtcggcattg atgataatta cattatcacc acaatcaacg tgtggggtgt
     2821 agctgggttt gtgtttcccc cttaggattt ttgctacttg gctcgatagg cgacctagca
     2881 cctggtcagt agcatcaatc acaacccact cctttttaac ggtggcatcg tttgccatta
     2941 ccgttttgta actttgcgta ttcactgcaa tctcctaatt tttaggttaa taccaacttt
     3001 tttgtgatcc tttaatttga taataagcgg gacgcaaaaa tacaactaat tatttaatta
     3061 gcaagggata gcttaaagaa aaatttttga ttgaactgaa gctgaagtgg ttagttttag
     3121 tgtttagggt atagtgttta gtgtatagta ggtttattat tattgtctag tcctaaagta
     3181 aggttccact taagtatgag ggagcaaggt atagcctcta agcctttatt cataactcag
     3241 gaattaatgc tgtttgattt aggatatttt tcatgtttag tcccgattat tcggaataga
     3301 ttccaacgaa acatctatct gtcatgctga gctagccgaa gtatctcttt gtacctttat
     3361 tccctttttt aaatgagatt cctcgactag ctcggaatga caatactttt gacatccacg
     3421 ttccctttgt catgctgagc ttgtcgaagc atctcttaac ctttaattct tgatgttttt
     3481 tgggcaattg atcttggcca tacttttctg ttgcgatgca acttttcagt gttgctcttt
     3541 ggaatgtaaa tgcgtgcatc gctgaacata tgggagtagc catcgggtta caaatatttc
     3601 gcccctacag ggctgggaag gttgtttgtt gttcgtgaaa ttgacctcac cccaagcccc
     3661 tctcctgaca ggagagggga gaagcaataa ggaaacctta ttgcagggct aaagtctccc
     3721 ctctcagggg agatttagag gggtcgttta ggttgaatat agaaaacggc ttcaccccag
     3781 cccttttcct gagagggaat ggagcggaag aagcaggcag tggcataaaa gcaagacctg
     3841 taaatgggtc gggcctttca gccttgatct tctttggttc tttcttgtat caagacatga
     3901 aagaacgtga aaaagctctt tgccatgctt atctgttgcg atgcaacgtt tcacccttgc
     3961 tctttggaat gccatagcgt gcatcgctat acaggtagga gtcgtgatgg agttacaaat
     4021 gtttagcccc tacggggctg gaaacggcaa tcgttgttcg ttattcgtga atcgttgttc
     4081 ttgaatgcca ttctgtgtaa caccgtgtta cactgagtaa ctctgtgata acaatttagt
     4141 tgatagttgt tggttgatag ttgatggttg atggttgata gttgatggtt gatagttgat
     4201 ggttgatagt tgatagttga tagttgatgg ttgatagttg atggcttaag aaaccacctt
     4261 cagtcatgaa tctgaaatct gaaatctgaa attttaaatt tttatccttt atcctttatc
     4321 ctttatcctt tatccttaga catcaccccc actgtacact gcacacggaa cactgtacac
     4381 ttaatatgag attcctccgc aaagtttatc ccggtatttc tgggatcagg ttggcaaaag
     4441 agcgtttttt gctagctgaa caacatttga attttcaagc tctcaaaggg atacgataag
     4501 agttttagtt gccaagaaaa atttagcagt caaaaactca tgtatcattt aaccaatccg
     4561 gcacttgtag cttgcgctat acttgccctt ggctatggca ttgagctttg agcggagcag
     4621 ctgcttgcgg atgggcgata cacggtcggt aaagagtatg ccatccaggt ggtcgtactc
     4681 atgctggatg atgcgggcag ccatgccctc atacacttcc tcgtgctcca caaatttctc
     4741 gtcgtggtat cgaatgcgaa ccttcgactc ccgttcaaca tcctcatgta agccgggtag
     4801 gcttaagcaa ccttcgttat aaattacttt ctcgccaaac cgttcagtaa tctgggcgtt
     4861 tatgaaggcc ttgcggaaat ccttaagggt tgggtcgtcg tcggcaaggg gggtgccgtc
     4921 aaccacaaaa agcctaatgg aaaggccaat ttggggggca gccaaaccaa taccatcgga
     4981 ttgctccatg gtttcgaaca tgtcagctat aagtgcatcg agctgggggt aatcgggtgt
     5041 tatattctga gccaccttgc gcaatactgg cgatccgtaa acgtaaactg ttcgtatcat
     5101 ctgtcctaat taacgcgtaa aattactact tatattttaa tccctccatg tagctttgca
     5161 ggataatggc agcgctgatt ttatctacat tgctcttttc ctgcctgtcc ttcttcttca
     5221 tgccaccatc aatcatggcc tgcaaggcca ttttggaggt aaacctttcg tccacaagtt
     5281 caatgggcat ttcgggaaac ctctttttga tggcgttaat aacgggcttt atgtagttaa
     5341 cagcttcgga aggggtattg ttcatttggc ggggattgcc cactaccagt atgtcgatgt
     5401 tttccttcag gaaaagttca gtaagataat caattacctt atgggtgggt atggtttcca
     5461 gcggcgatgc aataattcgc aacggatcgg tcatggctat gcccgttcgc ttacgaccaa
     5521 tatcaagtgc aacaattcta cccaaaacag cagcttattt tgaaatattc tcgagctgat
     5581 gattcaatcg ttccagatac tcgcccctta cggtaattag ctcgtggttt ttcagctcta
     5641 tattactttc tggcttattt gaaatcttgc ggatcattcc aaggttaatc aggcagcttg
     5701 ggttgatgaa gctaaaatta cgttcctcaa gcaaagtgca aatgtcgcca aaagggttgt
     5761 tagccagaaa atggctgcca tttaccagct tgatttgggt tccgttagga acactctcaa
     5821 caaaaacaat ttcgccaaca tcaaggcact cgtacttgtc atttgttgaa accaccaact
     5881 tctggggaat aatctccttg atattctcaa agagggcgaa atacttgtta cggccatcaa
     5941 agtttgagta acggcgacgg ataacctttt ccaacgcttc gtggagttca tcaacctcaa
     6001 ttggctttag taaaaaatcg atagcgctgt acttaaaggc cttgatggcg tagttattgt
     6061 atgcggttat aaagataaca tcaaagttcc ggtgaggaca accctcaagg aaatcgaagc
     6121 cgctacctcc tggcatttcg atgtcgagga aaacgatatc gggatcatat ttggttacct
     6181 tatcgatacc ttcactggca gaataagcaa caccaataat ctcaatatgc tgctcaaaat
     6241 gtcctaggag cagctcaagc gtacgtaacg aggcaatctc atcgtcaaca attagggcct
     6301 taagcatcgg gttaaaattt catcgtgcaa aaataagaat agttacattc attttcaaat
     6361 gtattggtat tacagcaaaa cctgacgttg tataggtata atgattacag aacaagatat
     6421 tccgaattaa aatatcgact taatatcatt cattaacctg ttgaaatggt tattgatgag
     6481 ttcaaactca tttctattcc tcatcgcaaa aatcaaaata ctggttaatc ctctaatctt
     6541 atagaacaac ttacgccagt agtaaagaat aactgctgcg ggaggtagta aaacaaggta
     6601 aagcaatgcc attgctaagc tttttagaat cagtccaaca attactgttt ggattaggta
     6661 tgcaagtggc aataacagca agcccgcagc atggcggact gaactgtgga actgaacgtc
     6721 ctcaaatttc ttacttagct ggtagggaat aattataggt atgatatggt tgatgaaacc
     6781 aaaaatggcc aaaggcgaca atacgacaaa aagtggaacc atccaaagca ggttaaaagc
     6841 tttacttacc tttagcggca aggcttgggg tttaatccgg ttggctgaaa tgcgatccct
     6901 cagcacatgg gctaactgaa taatctcatc gaaggtactc ctgtccgttt ccttaagtct
     6961 gtcgagtttt aaaatcaacc ttttgcttat ttcaaatgcc tggtttagag tattgggtag
     7021 tttgttctcc ttgataaacc tttttgggaa aagctgggtt aggaaaaggt actcgtaata
     7081 gttctgttca tcttcaatat gaatcatttc cgctttaatg cgctctgata gttcatctat
     7141 cagggcattg taggcaaggg ctttgttttg cctgtaaagg tcaaggtatt tcttaacagg
     7201 gaaaggttca ccaatccgga tgagcaattt gctcctatac ctaacatagt ttgagtagtc
     7261 cagcccaaca gggacaatat gaatgttaag tgagccatcg gaagcatcct cggtttgaag
     7321 ggcaatgcga gcaataccct tctttagcgg tcgtaatatt ttgaaaccag catggttacc
     7381 ctcggggaga attacaagac cgtttccttt acggattata tccatggttt ttaggaaaat
     7441 actatcgtta agtgagaggt tctcaaaacc atcgcgaatg cggtaaacgg gcatgatttt
     7501 tagaaaggta agtatttttg ataccagcgg tttcttaaaa atatcggcac gggcaaggaa
     7561 aatgggctgc cagctatgtt taagggttaa catggcaagc gcatcaatta gcgcattttg
     7621 gtggttgggg gcaaaaataa aggtagtgtt tggcggaagc ttttccatgc caacaatctc
     7681 ggttttaaag tagagcttaa acagaaagtt ttggtaaaac cttaaaagcc agtatgcttt
     7741 tgaccattcc tcaattcgtt cacgattggt gggcatagct atgttggatt aaaaacaaaa
     7801 atacatttag aggggttttc ttgaccagta ataggcaatc ccaagtcccg atactatatg
     7861 ccatataccc caaaaggcag cgataaaggc cattccgcca agtccatcga ataggttggg
     7921 gttaaagatt agcacaagtc caagtcctga attctgaata ccggtttcaa ttgctatagt
     7981 tcttcggtct tgcttgctaa gcttaaaaat gcttgggata gtatagccta ccagtaatcc
     8041 taatccatta tgtacaataa ctattagagc aatcagatgg atatatttca ggaagtaccc
     8101 aaagttagca gccagcgcgg caacaacaaa ccccaaaaat gccacaatgg aaaaaatctt
     8161 aaacggtttt gaaatgcgtt ccgcagtatt tgggaagaac cgggcaaagg cgataccgag
     8221 tataaccggt attccaagca gtatcagaac agttctaatc atttcccaag catcaatctc
     8281 aatgggtatt accaggttcg aggccgctga gtaaagtttt ccccaaaagg caaagttgaa
     8341 gggtgtcata aatattgccg caagggtggc aatggccgtc aaagtaattg acagtgccac
     8401 gtttcccttt gccaacgatg aaataaagtt agatatattt cctcccggac aagctgcaac
     8461 aagtatcata cccattgcaa cgcttggtgt tggttgaata attaatacta gtagaaatgt
     8521 aaacaaaggt aaagcaagga actgtgaaag gaaaccgaga ataacaggtt taggactaac
     8581 cagcaacagc ttaaaatgct ctatcttgat ttgaagtgcg ataccaaaca ttattaaagc
     8641 cagggtgatg ttcataaaaa gcaaaccacc ctttgtgaag tttagccata tccaatcaag
     8701 tgtctcaagt gtggctttta ggtgaacctc agttttaata acttttccgt tggctatttc
     8761 aatacgatgc ctgttggtgt agtaccaatc ctctcccttt actatactat cgggttcacc
     8821 agcgcgctcc agcgcttcag aaatattcat tccaatcctg agcgatgata tgggagtttc
     8881 ctttttctgt tcggctttaa gttgcctttt cagcttaatt tttagttttt tatgaggttc
     8941 cgctagccga atatccctta taacgctgtc aataaaaacg gcctgattcc cttggtagaa
     9001 atacctggaa accacctgtt tttctgttgg cgagccaagt gttttggtaa caaaagctga
     9061 atcgctacct tgtgggatgg tttttatctt tttggcaatg gtttcagcgt ttgctaacga
     9121 tgatagcagt attaccaata agaaaactaa cagcttttgc gtaatcgttg atactctttt
     9181 catgcttagt aggtttagtg ttttttaaca aacccatacc ctgcaaaggt tactattttt
     9241 tttcaaatag caattgtcaa aaatttatat caaaataaga ataaagcaat tttctgagtt
     9301 ccaaatcgta aaaaaaagta tatctttgcg ccgctaaagt aaaatagtag gtttaactaa
     9361 aagcgtaaag tatgcctgta aaaatcagac taagccgcca gggaaggaag aaaagacctt
     9421 tctaccacat tgtggtggcg gatagcaggg cgccacgaga tggcagattc attgagagga
     9481 ttggttcata caatcccctt acaaatcctg ctactattga attaaatttt gatagggcat
     9541 tggactggct gcaaaaaggc gctcaaccta ctgacacctg tagggctatc ctatcgtaca
     9601 aaggtgtaat gctaaagaaa caccttcttg agggtgttaa aaagggtgct cttacccccg
     9661 agcaagctga ggctaaattc caggattggg taagccaaaa ggaagctaag attaaggcta
     9721 agattgattc attggccaag ggcgaagccg aggagatgaa gaaacgcatg gaagccgaat
     9781 cgaaggttcg tgatgctcgc gctcaggaaa tagccaaaaa gcgcaccgaa atggccgcca
     9841 aagaagccga ttctcaagca gaatcaactt cagaggaaac cgaaaacgct taggcttaag
     9901 gtgcatgggg gatgccggtt tcgttgagat tggatatatc caaagaacac atggcattaa
     9961 cggtgaactg gcggtgagtt taaactcatc ggttgagttc aaccccgaag aattggaatc
    10021 ggtatttctt gaaattgaag gaataccggt tccttttttt attacccgta ttcgtttcca
    10081 aaaccctgaa aaagcaattg taaagttcga tgatgtggac tctattgacc aggcgcagga
    10141 actttacggt gttaaaatgt taatcccttc caccagtata gagctggagg acgaagttta
    10201 cctttccgat ttagttggat acaaagtgcg gaatactgat aaatcggagg ttggagtaat
    10261 agttgactac actgagtact ccatgaatgc aacgtttgag ctggttaccc ctgatggtaa
    10321 acatgtactg atcccagcag ctgacgaact gattgtggag gttgatactt cggctaagct
    10381 gcttgaaatg gaacttcctg aagggttgat tgatttaaac ctatagctgt aaatctttct
    10441 caaagcttaa tattaactcc ctccaacggt gcgggatggg aatggatttg ccggccgcat
    10501 tgctgaaaca aaccattaca gctttgctta ctgcggccac ttcaccggta gtgttgttaa
    10561 aaatctcctg ccgcattttc aggcttttgt tgcctatttc ataaacctta cttaatacct
    10621 caatattatc ccacatgcgt atgggaataa ggtaatcgat ggtgatactc gccagtacca
    10681 gcccctctat ctcccagttc attttttcct gtagaacctc agtaaaatat ttcaacctgc
    10741 ctaggtcaaa gtactgctgg taaaccgagt tggttacgtg tgaaagctga tcgatatcgt
    10801 taaacctgat ttggatgggt gtacggtgga aaaatttatg cagttttgga ttgctgctca
    10861 tttctgtact ttttttgctg gtcgatttct tcgaaaatcg agtttcggct tacgaattcg
    10921 gggacaatat ccttcattat acttactatc ctaaaattat tgtggcttgg aagagtgttt
    10981 aatagttcca ttattttagg catcacctct tcctgcttat actcgttaac tttagcaatc
    11041 ataatcttag ggtggtaggt agggatggtg ttttccttgt tgttaagtag ttcctcatac
    11101 agtttttccc cgggtctaag tccggtaaag ctgatttgaa tatccttacc ataggtaagt
    11161 cctgagagct taatcatttt cttggccaga tcgagaatct ttactggctt gcccatgtcg
    11221 aaaatgaata tttcaccgcc ttgccccatt gcccctgctt caagaacaag ctgacaagcc
    11281 tcggggatgg tcatgaagta cctgataatg tccggatgtg taatggtaat tggtccccca
    11341 ttctcaattt gcttcttgaa gcgtggaatg accgatccgt ttgaacccaa aacatttcca
    11401 aaacgggtag taataaactt tgtttgggaa gtatagttca gcgtttgggt gtatatctcg
    11461 gctatacgtt tgcttgcacc catcacattg gtggggttta ccgctttatc ggtggaaatc
    11521 attacaaatt tctcaacgtg aaatttaacg gctatatcgg caacgagttt tgttcccaaa
    11581 acgttgttaa acactgcttc ggtaggatta ttctccatca tgggaacatg cttgtaggca
    11641 gcagcatgga ataccacttg tggcttaaat gcctcaaaca gtctgttcac cctgtactcg
    11701 tttgtaacgc ttccaataac agtttcaaag tcgaaaaact gaaaagtttc cttcagctca
    11761 agctccaatt cgtata
//
LOCUS       DAOOWJ010000080        11736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5435_length_11736_cov_6.787261, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000080 DAOOWJ010000000
VERSION     DAOOWJ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5435_length_11736_cov_6.787261"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..75)
                     /locus_tag="PLW22_08420"
     tRNA            complement(2..75)
                     /locus_tag="PLW22_08420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Thr,seq:tgt)
     gene            complement(295..594)
                     /gene="raiA"
                     /locus_tag="PLW22_08425"
     CDS             complement(295..594)
                     /gene="raiA"
                     /locus_tag="PLW22_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOU74768.1"
                     /translation="MNVKIQSIKFDADRKLLDYVEKKAAKLERYFDNIVETEVYLKLE
                     NSQDMENKVVEFRLKVPGNDLFAQRKGKTFEEATDLCIDVLKGQVKKFKEKLRGV"
     gene            complement(615..1499)
                     /locus_tag="PLW22_08430"
     CDS             complement(615..1499)
                     /locus_tag="PLW22_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOU74769.1"
                     /translation="MDMLASFLKYLDIEKGYSSHTIRSYGDDLHSFFSYSGVNPASTE
                     AIEHISHRTIRAWMSNLVSQGLSTRSVNRKLSSLRTFYKYLLRQGVIANNPMKRVVSP
                     KTSKRLPEFVPEADMAKLNTNDLFGDDFEGLRNRLIITMFYFTGMRLSELVGLTLQSV
                     DVDSMTIKVMGKGSKERIIPIHPDLKDLVVEYLEERNKKFSDTVSFFVTPKGKPVYPK
                     LVYRVVNHYLTLITPLQKRSPHVLRHSFATHLLNKGADLNAIKELLGHSSLLATQVYT
                     HSTFEKLKNNYNQAHPRA"
     gene            complement(1587..1778)
                     /gene="rpsU"
                     /locus_tag="PLW22_08435"
     CDS             complement(1587..1778)
                     /gene="rpsU"
                     /locus_tag="PLW22_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOU74770.1"
                     /translation="MIIVPVKEGENIERALKKFKRKFEKTGIMRELRSRQAYVKPSVA
                     RREQIKRAIYIQKLQQQEE"
     gene            complement(1889..2794)
                     /locus_tag="PLW22_08440"
     CDS             complement(1889..2794)
                     /locus_tag="PLW22_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU74771.1"
                     /translation="MKRIWGSVKSFLYFSRGERNGTIILVLLVLLLAVAPYLYKKYFN
                     VVSPPDPMLVQKADSFFLTLQYHEPEKVIEPPSGIEAEMELPRTKKSFLFDPNTVSLD
                     SLMELGLSRKQAEVLVKYRSKGGRFRTPDDIDKIFVIDSATKARLKPLVRIAPVTDTI
                     NHDRVIDTKSILVELNSADTLSLLKLKGIGSNYAKRIVGYRNLLGGFYSIDQLGEVYG
                     FTPQLVDQLRPNIYIDTTRIRKININLIKYDDLRIHPYVNDYQAKAIIYYRSQKGTIK
                     TLDELWKNKLLPRDRFEKLKHYLDL"
     gene            complement(2861..>4210)
                     /locus_tag="PLW22_08445"
     CDS             complement(2861..>4210)
                     /locus_tag="PLW22_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HOU74772.1"
                     /translation="FVVMWLMVWGVYFTFRMGFINIRAFWHSIQIISGRLDKNSKKGE
                     VSHFQALSTALSATVGLGNIAGVAVAISVGGPGATFWMIMAGLLGMSLKFTECTLGQK
                     YRLIDSKGRVSGGPMYYLSQGLAKRNMKTLGNILAVTFSVLVIGASFGGGNMFQANQA
                     FSQMAFIIPWLNGKGAYFGIVMALLVGLVVLGGIKSIASVASRIVPFMVILYISASMV
                     IIFLNISRLGEVFSLILEQAFGLKSIAGGFFGAFIVGFQRGAFSNEAGIGSASIAHST
                     ARTDEPVSEGIVALLEPFIDTVVVCTMTALVINFAGLAGNSSGLQGAQLTSAAFATVL
                     PWFPYLLAIAILLFAFSTMISWSYYGLKGFNYLFEKRLNGIIPVPEASTKLYYIIFLL
                     CIVVGSASNLWAVMDFSDMMILGMAFPNILGMIFMAKEVRADLDSYWNRWKTGRLYR"
     assembly_gap    4211..4310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4311..5512)
                     /locus_tag="PLW22_08450"
     CDS             complement(<4311..5512)
                     /locus_tag="PLW22_08450"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOU74773.1"
                     /translation="MSVSNNRDSFGSKFGIIAAAAGSAVGLGNIWKFPYITGQNGGGA
                     FLLIYLLFVFLLGIPVMISEFSIGRRGQRNVFGSFKAISPGTPWYFVGIMGVVAAFVI
                     LSFYSAVAGWTLEYIVKSVSVGFGNQTTQQLGDAFQNFLVHPVRPIIWQLVFMALTAY
                     IVIGGIKNGIEKYNKFLMPLLVVFLLILCVRSITLPNASKGLAFLFKPDFSKVTIKTF
                     LFAMGQAFFSLSLGMGALITYASYFRKTDNLGRTALNVSLADTFIAVLAGVVIFPAVF
                     AFNIEPTAGPSLVFITLPEIFSNIPMGNLFGFIFFILLAVAALTSTVSVLEVVVAYFS
                     EELRINRLKATILASVSISVLGVLASLSFGPLSNIRLSGKTIFDMLDFLASNILLPLG
                     GMLIVIFVG"
     gene            complement(5646..6704)
                     /locus_tag="PLW22_08455"
     CDS             complement(5646..6704)
                     /locus_tag="PLW22_08455"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOU74774.1"
                     /translation="MNYDKIVELLGDNANYLLEHKCNTVPKEMLHLPGPDFMERVVYH
                     SNRSPQVMRSLQAMFYHGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVKL
                     AIEGGANAVASTFGVLASVARKYAHRIPFIVKINHNELLTYPNKYDQIMFGSVDEAWN
                     LGAVAVGATIYFGSEESNRQIIEVAQAFERAHELGMVTVLWCYLRNNGFKKDGVDYHV
                     AADLTSQANHLGVTIQADIIKQKAPENNGGFKAINFGKTHDKVYSQLTTDHPIDLTRY
                     QVVGCYNGRAGLINSGGASSGASDLAEAVYTAVVNKRAGGMGLISGRKAFQRPMDEGI
                     KILNAIQDVYLESKVDIA"
     gene            complement(6773..>8562)
                     /locus_tag="PLW22_08460"
     CDS             complement(6773..>8562)
                     /locus_tag="PLW22_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU74775.1"
                     /translation="ERERIETMLKRNGYFTFSKNFITFTADTNFRNNRVDLNLIIKNP
                     LRADENGKLKPIRFKRYKFRNVFIYPNYDPFKFSSYRDNGELDTICRASVYYVFHQNP
                     GVKLDVIEAFNQLKPNEMYSVDQVNRTQQNLSQIRLFKFVNIELEDVEAKEQSGEIDF
                     SDLDTGSESEEFGYLNCYIQLVPHTLQSYQVELVGTNTSGSLGAEGTLNYQHKNLFKG
                     AEVFDIKFRGLVETAQQKINLNNTLELGGSMGLTTPKYIGPYTSHVRVDKYAPSTQLT
                     ASYSFQRRPEYTRMIAGLQFGYVWKSSRFMLHTLNPIELNAISIQKISPEFQAQIDTT
                     FLKYSYISQIVTVSSYNMVYSNQNVKKASSYSYIRFNLELSGNALQAISKLSDRPKNS
                     NGAYTFFNTEFSQFVRSDINLTYHQVADENNSFAYRLYIGVGYPYGNSRALPFEKRFF
                     TGGANGVRAWQARSLGPGSYYQPAERFPNRTADIKLEANFEYRFRMVWKLEGAMFIDC
                     GNIWSMPGLDDRTGATFEWNKFYEQIAIGSGLGIRLNLGFFILRTDFGYKIFDPAINP
                     NEPYKPWVPFNRKFSISDITFNFGIGYPF"
     assembly_gap    8563..8662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8877..9626
                     /locus_tag="PLW22_08465"
     CDS             8877..9626
                     /locus_tag="PLW22_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU74776.1"
                     /translation="MLSRNSIKRVKSLQQKKFRNELGLFIAEGTKLVDELISSNFEIE
                     EIYHTEQYLVTNVNCLVHKVSQSEMERISGLVTPSPVLAVVRFPTYKLDVDPSKELVL
                     ALDTIQDPGNMGTIIRLADWFGIDNIVCSHGCADAFAPKAIQATMGAIAHVKVHYTDL
                     ITWLSQHTVNCPIIGACMDGENIYKAKKEHAGIIVMGNEGNGISPAVDKLITHRIHIP
                     TFARGRQKVESLNVAMATAIILSEFKGTLHQ"
     gene            complement(9654..>10052)
                     /locus_tag="PLW22_08470"
     CDS             complement(9654..>10052)
                     /locus_tag="PLW22_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOU74777.1"
                     /translation="FWHDLLEILLEMKNIRRVNVKVKTLEEKQKEVEFDERFAEQPVV
                     SIPLEAKPEVKEAKPSLDRIKKLHLKLRMEAFQIYRELIKQRTPERITDTLRTFQEQE
                     MLYANGRTKPGKIVTKAKPGQSYHNYGLAP"
     assembly_gap    10053..10152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10153..11381)
                     /locus_tag="PLW22_08475"
     CDS             complement(<10153..11381)
                     /locus_tag="PLW22_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU74778.1"
                     /translation="MPHDISIVLRPDEAANETAVVRAIARKIDIPTDSIRHYYITRKS
                     IDARKGKVLVNLCVRVFIGEEKIIDERPIFNYNDVRNAMPVIIVGAGPAGLFAALRLI
                     ELGFKPIVIERGKSVSHRKRDIAMLNREHQLNPDSNYAFGEGGAGTFSDGKLYTRSKK
                     RGDYRRILQALVDHGADPAILYEAHPHIGTNRLPGIITRIRETILQYGGEVLFNSRVT
                     ELILDSGQAVGVKLADGQRLHGKAVILATGHSARDIYHMLHNQSIALEAKPFAMGVRV
                     EHPQALIDAIQYHGIPRSEYLPAAAYSLVHQVEGRGVYSFCMCPGGFIVPAATGTNEV
                     VVNGMSPSDRNSRWANSGIVVEVRLDDLAGYAQHGIFAGLEFQAELERLAKQHGGYGV
                     QAPAQRLVDFVEGRFSS"
     gene            complement(11438..>11736)
                     /locus_tag="PLW22_08480"
     CDS             complement(11438..>11736)
                     /locus_tag="PLW22_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74779.1"
                     /translation="NVRPYLYLGTDVRIDLAAYKRLKVEKGVLLRLQKFDYYYEVGFG
                     VDFFLQYFKFSAEIKWSAGLLNMLSSDYVDEGKIYHDALGSLRSKFLIVSFHFE"
BASE COUNT         3430 a         2540 c         2271 g         3195 t
ORIGIN      
        1 gagccgatgg agggattcga acccccgacc agctgattac aaatcagctg ctctggccaa
       61 ctgagctaca tcggcaatca aagtgttttt gcactatttt aaggaacgtt tacctcctga
      121 aatcggattg caaatgtata acaattttaa ttatctgcaa aaatttttat ctttttatgt
      181 aacctttttt agtacaattt tcataatcca tctgtctgtt ttgacctgtt acagcaaaaa
      241 atgaagccct ttcgggcttc atctgttttg tttttagtct attttttggg tttgctatac
      301 ccctcttagc ttttccttaa actttttaac ttgacccttc agcacatcaa tacataggtc
      361 agtagcctct tcaaaggttt tccctttacg ttgggcaaac aaatcgttgc caggcacctt
      421 aaggcggaat tcaacaacct tgttttccat gtcctggcta ttttccaact ttaggtacac
      481 ctcggtttcc acgatgttgt caaaatagcg ctcaagtttt gcagctttct tctcaacgta
      541 atcaagaagc ttccggtcag catcaaactt gatggattga attttaacgt tcataataaa
      601 tcctcctttt ggttttaggc tcgtggatga gcctggttat aattgttttt aagtttttcg
      661 aatgtgctgt gggtgtacac ctgggtagcc agtaagcttg agtggccaag cagctctttg
      721 atggcattca aatcggcacc cttatttagc aaatgggttg caaagctgtg ccttagcacg
      781 tgggggctcc ttttttgaag tggtgtaatc aaggttaagt agtggtttac tactctgtat
      841 acgagttttg ggtaaaccgg tttacctttt ggcgtaacaa aaaaactaac agtatcggaa
      901 aattttttgt ttcgttcctc aaggtactcc acaaccaaat ctttaagatc cggatgaatg
      961 ggaatgatac gttccttgct acccttcccc attaccttaa tggtcattga atccacatcg
     1021 accgattgga gggtaagccc aactagctcc gataggcgca taccggtaaa atagaacatg
     1081 gttattatta gccggttacg taagccctca aaatcatcgc caaacaggtc gttggtatta
     1141 agttttgcca tgtcggcttc aggtacaaat tcaggtaagc gcttactggt tttgggcgaa
     1201 actacacgtt tcatgggatt gttagcaatt acgccttggc gtaataggta cttatagaat
     1261 gtgcgaagcg atgaaagttt acggttaacc gaacgggttg ataggccttg gctaactaag
     1321 ttcgacatcc atgccctgat ggtcctatgg ctaatgtgtt caatagcttc ggtacttgcg
     1381 ggattgactc ctgaatagct gaagaaggaa tgcagatcat cgccatacga gcgtatggtg
     1441 tgtgatgagt aacccttctc tatatcaagg tatttaagaa atgaggccag catatccatt
     1501 gaatgcaata tagttttttt caaactaaaa agcaaaaaaa aatcaggcaa aatagcctga
     1561 ttatgcattc aactgaacat cagcatctac tcttcctgct gctgtagctt ctgaatgtaa
     1621 atggctcttt tgatctgctc cctacgagca accgaaggtt taacgtaagc ttgacgggaa
     1681 cgtaactccc tcataatacc ggttttttca aacttacgct tgaatttttt tagagctctt
     1741 tcaatatttt cgccttcttt aactggtact ataatcatgt ttatcgaatt ttatgagctg
     1801 caaaattata atgttttgat tttaatacaa aatattttag tctttttttg tggaaattat
     1861 tccaattgtt tgtgaagtat caattttact ataaatcaag gtaatgtttt agcttttcga
     1921 accggtcgcg aggtaaaagc ttgttcttcc atagctcatc aagtgtttta atggtacctt
     1981 tttgtgagcg gtagtaaata attgctttag cctgataatc gttcacatag ggatggattc
     2041 tcaggtcatc gtacttaatt agatttatgt tgatttttcg aatacgggta gtgtcgatat
     2101 atatgtttgg ccttagctgg tcaaccagtt gcggagtaaa tccataaacc tcgccaagct
     2161 gatcaatact ataaaatcca ccgagtaggt tcctgtaacc cacaatccgc ttagcgtagt
     2221 tacttcctat acctttaagt tttagtaacg agagggtatc ggcactattg agctccacaa
     2281 gaatggactt ggtatcaatg accctatcat ggttaatagt atcggtaacc ggagctatac
     2341 gtaccagcgg ctttagcctt gcctttgtag ccgagtcgat aacaaaaatt ttatcgatat
     2401 cgtcaggagt cctgaaacgc ccccctttag acctatactt aacaagtacc tcagcttgct
     2461 tccggcttaa ccccaattcc attaaactgt cgaggcttac ggtgttggga tcaaataaaa
     2521 atgatttttt agttcgggga agttccatct cggcctcaat acccgaaggt ggctcaataa
     2581 ctttctcggg ctcatggtac tggagagtta aaaagaaact atcggctttt tgaacaagca
     2641 tgggatcggg aggggaaaca acattgaaat actttttata caggtacgga gccacagcca
     2701 agagcaaaac taatagaacc agaataatgg ttccatttcg ttcgcccctt gaaaagtata
     2761 agaagctttt aacgctaccc caaatccgct tcattgttag caaccagttc tactaatacc
     2821 aaatgtcgcg aaaaatgata aaagaatcaa taagtttacg ctacctatac aatctccctg
     2881 ttttccatct attccagtaa ctatccaaat cggcccgcac ctctttagcc ataaaaatca
     2941 ttccaagaat atttgggaaa gccatcccta agatcatcat atcggaaaaa tccatgacag
     3001 cccaaaggtt cgaagcgcta cccacaacaa tacaaagtaa aaaaatgatg tagtatagct
     3061 tggttgatgc ctcaggtaca ggaataatcc cgttcaaacg tttctcaaaa aggtagttaa
     3121 agcctttgag gccatagtat gaccagctga tcatggttga aaaagcgaat agtaagatag
     3181 caatagccag cagatatggg aaccaaggta atacagtggc aaatgcggct gaagttagct
     3241 gggctccctg taaaccagaa ctattaccag ctaaaccagc aaaatttatt actaaagcgg
     3301 tcatggtgca taccactaca gtatcgatga atggttcaag caaagctaca atgccctcac
     3361 taacaggctc atcggttcgg gcagttgagt gcgcaatgga agcggaacct atccctgcct
     3421 cgttggaaaa tgctccccgt tgaaacccaa caataaacgc accaaaaaaa ccaccagcaa
     3481 tggatttgag tccaaatgcc tgctctaaga tcagactaaa aacctcaccc aaccgtgaaa
     3541 tgtttagaaa gataattacc atggatgctg aaatgtagag gataaccatg aaaggaacta
     3601 tccgcgaggc aacactggca atgcttttta ttccacccag gactactaaa cccaccagca
     3661 aggccatgac aataccaaaa taggctccct taccatttaa ccagggtatg atgaaagcca
     3721 tttgtgaaaa tgcctgatta gcctgaaaca tatttccacc gccaaacgat gcgccaatta
     3781 ctaaaaccga aaaggttact gccagtatgt tacctaaagt tttcatgtta cgcttggcaa
     3841 gcccttggct caggtagtac attggccctc ccgataccct accctttgag tcaatcaatc
     3901 tatacttttg ccctagcgtg cactctgtaa atttaagcga catgcccaac agcccagcca
     3961 ttatcatcca gaatgttgcc ccgggccccc caacgcttat ggcaactgca acacccgcaa
     4021 tatttcccaa accaacagtt gctgaaagtg cggttgataa ggcttgaaaa tgggatacct
     4081 ctcccttttt gctattctta tcgagcctac ccgagatgat ttggattgag tgccaaaaag
     4141 cccggatgtt gataaatccc atgcggaaag taaaatagac tccccaaacc attagccaca
     4201 ttacaacaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccaacaaaaa
     4321 tcactattag cattcctccg agtggaagta agatgttaga ggccagaaaa tcgagcatat
     4381 cgaaaatggt tttgcccgag agcctgatat tgctcaaagg cccaaatgat agtgatgcta
     4441 aaacacccaa taccgatatg gaaaccgaag ctaaaatggt tgccttaagg cgattgatac
     4501 gcaactcctc tgagaaatat gccacaacca cttcaagcac cgataccgtt gaagttaacg
     4561 cagctacagc aagtaaaatg aaaaaaataa acccaaaaag attccccatt ggtatgttgc
     4621 taaaaatctc gggtagggtt ataaaaacca aacttggacc tgcagtaggt tcaatgttaa
     4681 aagcaaatac agcaggaaat atcactactc ctgctaaaac tgcaataaat gtatcggcta
     4741 atgatacgtt taatgccgtt ctacctaaat tgtcagtttt tcggaagtat gaagcataag
     4801 taattaaagc acccattcca agactaagtg agaaaaaagc ctgccccatg gcaaatagaa
     4861 atgtctttat ggttaccttg gaaaagtcgg gcttaaacag gaacgccaat ccttttgatg
     4921 cattgggtaa ggtaatagat cgtacacata gtattaaaag aaaaactact agcaatggca
     4981 ttaagaactt gttatatttc tcaataccat tttttattcc tcctattaca atatatgcag
     5041 taagtgccat aaatactaac tgccagataa tgggacgaac aggatgaacc aaaaagtttt
     5101 gaaaagcatc acccagttgc tgcgttgttt gattaccaaa tccaacagat actgatttta
     5161 ctatatactc cagtgtccaa ccagccacag cactataaaa cgataagatt acgaaggcgg
     5221 ccaccacccc cattataccc acaaaatacc atggagtgcc aggcgaaatt gccttaaatg
     5281 aaccaaatac attgcgctga ccacgcctgc caatggaaaa ttcagatatc ataacaggga
     5341 ttcccaaaag gaacacaaac agaagataga tgagcaaaaa agctcctcct ccattctgcc
     5401 ctgttatata tgggaatttc caaatgtttc ctaatcctac tgccgagccg gcagcggctg
     5461 caataatacc aaatttgctt ccaaagctat cgcgattatt agaaactgac ataggttttg
     5521 agtttaggtt taggttataa attttggcgc accaatttgg aaaaaaatgg acactaaaac
     5581 aagtgttagc gtaaaaaaag cccatcccgg acttcagaat gggcaatgtt ttgaaaaagc
     5641 atttgttaag ctatgtcgac cttactttca aggtaaacat cctgtatggc attcagaatt
     5701 ttaataccct catccatagg gcgttgaaaa gctttgcgac cgctaatgag tcccattcca
     5761 cctgcacgtt tattaactac agcggtataa acagcctcgg ccaaatcgct tgctcccgag
     5821 gaggcaccac ccgagttaat taaaccggct cgtccgttat agcaacctac tacctggtag
     5881 cgggtcaaat caatgggatg atcggtagtt agctgtgagt aaaccttatc gtgggttttt
     5941 ccgaagttta tagctttaaa gccaccatta ttctctggag ctttctgttt aataatatcg
     6001 gcctgaatgg taacccccaa atggtttgcc tggctggtta agtcggcagc tacgtggtag
     6061 tccacgccat cttttttgaa tccattgttt cgcaggtagc accaaagaac ggtaaccatt
     6121 cccaactcat gggcacgctc aaacgcctgg gcaacttcaa tgatttgccg attgctctcc
     6181 tcggaaccaa aatatattgt agccccaact gctactgcac ctaggttcca tgcctcatca
     6241 accgatccaa acataatctg atcgtactta ttgggatagg taagcagctc gttatggtta
     6301 atcttaacaa taaaaggtat gcggtgggca tatttacggg ctaccgatgc cagcacccca
     6361 aatgttgaag caaccgcatt agcaccaccc tcaatggcca acttaacaat gttttcagga
     6421 tcaaagtaaa tgggatttgg ggcgaacgat gcacctgcac tatgttcaat tccctgatca
     6481 acaggcaaaa tggacagata ccctgtacca gccaaccgtc catgatagaa catagcctgt
     6541 aagcttcgca ttacctgggg cgatcggttg ctatggtaaa ccacacgctc catgaaatcg
     6601 ggcccaggaa gatgaagcat ctcctttggt actgtattgc acttatgttc aagaagataa
     6661 ttggcgttat cgccaagcag ctcaactatt ttatcgtagt tcataggtat agaattttat
     6721 cggttctata caaacctacg aaaaatattg gtttaagtca agtagagtaa agctaaaagg
     6781 gatatccaat accaaaatta aaggttatat cgctgatgct aaactttcga ttaaacggaa
     6841 cccagggctt gtatggctca tttggattta ttgccggatc gaaaatttta taaccaaaat
     6901 cggttcgtaa aataaagaaa cctaaattca accttatccc cagtccacta ccaatggcaa
     6961 tctgctcata gaatttattc cattcaaagg tagcccctgt cctatcgtca agtccgggca
     7021 tcgaccaaat attgccgcaa tcgataaaca tagccccttc cagcttccaa accatacgga
     7081 agcggtactc aaaatttgct tccagcttta tatcggcagt gcggtttggg aagcgctcag
     7141 cgggttgata gtatgagcct gggcctaatg agcgcgcctg ccatgctcgt actccattgg
     7201 ctccacccgt aaaaaatcgc ttttcaaagg gcaatgcccg tgagttaccg taaggatagc
     7261 caaccccaat gtacaaacga tatgcgaagg agttgttctc atcggcaacc tgatggtatg
     7321 taagatttat atcgctccga acaaactgtg agaactccgt attgaaaaac gtatacgccc
     7381 cgtttgaatt ttttggtcta tcgctcagct tcgatattgc ttgaagagcg ttccccgaca
     7441 gttcaagatt aaagcgaatg taactgtagc tgcttgcctt tttaacgttt tggttactat
     7501 aaaccatgtt gtagcttgaa actgtcacta tctggcttat gtagctgtat ttaagaaagg
     7561 ttgtatcaat ctgagcctga aactctggac taatcttttg aattgaaatg gcatttagct
     7621 caattgggtt aagggtatgt agcataaagc gtgagctttt ccaaacataa ccgaactgta
     7681 acccggctat catacgggtg tactcagggc ggcgctgaaa gctgtacgag gcagtaagct
     7741 gggtgcttgg agcatattta tcgaccctaa catgcgaagt gtaaggtcct atgtactttg
     7801 gggtagtaag ccccattgaa cctccaagct caagggtatt attcaggttg attttttgct
     7861 gggcggtttc aacaagtcct cggaacttaa tatcaaatac ctctgcgcct ttaaataggt
     7921 ttttatgctg atagtttaac gtaccttcag cgccaagcga gccgctggta tttgttccta
     7981 ccaactccac ctgatagctc tgcagggtat gcggaaccag ctgaatataa cagttaagat
     8041 acccaaactc ctccgattca gaaccggtat ctaaatcaga gaaatcaatt tcaccgcttt
     8101 gttcctttgc ttctacgtct tccaattcaa tattaacaaa cttaaataac ctaatttgac
     8161 tcagattctg ctgagttctg ttgacttggt caacagaata catttcgttt ggttttaact
     8221 ggttaaatgc ctcaattaca tcgagcttca cacccgggtt ctggtgaaaa acatagtaca
     8281 cactagccct acaaattgta tcaagctcac cattatcacg gtacgagcta aacttgaatg
     8341 ggtcatagtt cggataaata aaaacattcc gaaacttgta tcgcttaaac cttatgggtt
     8401 tcagttttcc attctcatcg gcccggagtg gattttttat gataaggtta aggtcaaccc
     8461 tgttgttcct gaagttagta tcggcagtaa aggttatgaa attcttacta aaagtgaagt
     8521 agccatttcg ttttagcatt gtttcaattc gctccctttc ggnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnaaagtcac aggcataatc agagttaggt attatgaact
     8701 atacaaatgt aattataaca gcaattcgcc atctattttt tacagcaata gttatggatt
     8761 tatgaccaaa ttcatcaaaa tcagggccaa aataaaattc gcacatggga tgctaagaat
     8821 ttgaaaatgc tgataatatt agaaaaatgt tttattttgc cctaaaattc aggtaaatgt
     8881 tatcaagaaa tagcattaaa agggtaaaat cgttacaaca gaaaaaattc aggaatgaac
     8941 ttggactttt tatagccgaa ggtactaaac ttgtagatga gctcatcagt agcaattttg
     9001 aaattgagga gatataccat accgagcaat atttagtaac aaatgttaat tgcttggtac
     9061 acaaagtaag tcagtccgaa atggaacgga taagcgggct tgttacacca agcccagtgc
     9121 ttgccgtagt tcggttccca acatataaac tggatgttga cccaagtaaa gagttggttc
     9181 ttgctctgga taccattcaa gacccaggca acatgggtac cattatcaga ttagctgatt
     9241 ggtttgggat tgacaatatt gtttgttccc atggatgtgc cgacgctttt gccccaaaag
     9301 ctatccaggc aaccatgggt gccattgccc atgttaaggt tcattatacc gatctgatta
     9361 cctggcttag ccagcacact gttaactgcc caattattgg ggcatgtatg gatggtgaaa
     9421 atatttacaa ggcaaagaaa gaacacgcag gcattattgt gatgggtaac gagggtaacg
     9481 gcatcagccc tgctgttgac aagctaatta cacatcgtat ccacatacca acattcgcta
     9541 ggggtcgcca gaaagtagaa tcgttgaatg tagccatggc tacagccata atactctcag
     9601 aatttaaagg gacattacat caataaatct acctgcggaa gaacggtaaa aacctacggg
     9661 gccaagccat agttgtgata agactgaccg ggtttagcct tggttacaat tttgcctggc
     9721 ttggtacgcc cattggcgta aagcatttcc tgctcttgaa aagttcgtaa tgtatcggtg
     9781 atcctttcag gtgttcgttg ctttataagt tcccggtaaa tctggaacgc ttccatccga
     9841 agtttgagat gaagtttttt aatacgatca agcgacggtt tagcctcttt aacctcaggt
     9901 ttagcctcta agggaatgct aacaacaggc tgttcggcaa atctttcgtc aaactcaact
     9961 tccttttgtt tttcttcaag cgttttgact tttacgttta ccctgcggat gtttttcatt
    10021 tcgaggagta tctcaagcaa atcgtgccag aannnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nntttgatga gaaacgccct tctacgaagt caaccaaacg ctgagcggga
    10201 gcttgaactc catatccccc atgctgctta gcaagacgct ccaattcagc ctgaaactca
    10261 agtccggcaa aaatgccatg ctgagcatag ccagccaagt catctaaacg gacttcaaca
    10321 actattcctg agtttgccca gcgtgaattc ctatccgagg gcgacattcc attgactaca
    10381 acctcattgg ttcctgtagc ggcaggcaca ataaaaccac cgggacacat gcagaatgag
    10441 taaacccccc gtccctcaac ctgatgtacc agtgaatacg cagcagcagg taaatactcg
    10501 cttcggggta tgccatggta ttgaatggca tcgataagtg cttgtggatg ttcaaccctc
    10561 acccccatgg caaacggctt agcctccaag gcaatacttt gattgtgaag catgtgataa
    10621 atatcgcgag ccgaatgccc tgtggcaagt attacggctt taccatgtaa cctttgccca
    10681 tcggcaagtt taacaccaac cgcttgacca gaatctaaaa ttagctccgt aaccctacta
    10741 ttaaaaagca cctcaccccc atactgaaga atagtttccc ttatacgagt tataattccg
    10801 ggtagcctgt tggtgccaat atgcgggtga gcctcgtata gtattgccgg atcggctcca
    10861 tgatcaacca gagcctgaag aatgcgcctg taatcgcctc gctttttgga acgggtgtat
    10921 aacttgccat cggaaaaggt tccggcacca ccctcgccaa aggcgtagtt tgaatcgggg
    10981 ttaagctgat gttcccgatt tagcatagct atatcgcgct tacgatggct tacggatttg
    11041 ccacgctcaa ttacaattgg tttaaaacca agttcaatta accttaatgc agcaaataat
    11101 cctgctggcc ccgccccaac aataataacc ggcattgcat tacgaacatc attgtaattg
    11161 aaaatcggtc gttcatcaat aatcttttcc tcgccaataa aaaccctaac gcaaaggtta
    11221 accagaacct tacccttacg ggcatcaatt gatttccggg ttatatagta atgccggatt
    11281 gaatcggttg gtatatcaat tttccgtgca attgctctaa caactgctgt ttcatttgcc
    11341 gcctcatcgg gccttaacac aatagagata tcatgtggca taaggaaaaa ctctaataag
    11401 aaagcattaa cacagataag gacaaaaagg tttaaaccta ctcaaagtgg aacgaaacaa
    11461 tcaggaactt cgatcttaaa cttcccaggg catcgtggta aatctttccc tcatcaacat
    11521 aatcgctcga taacatatta agcaaaccgg ccgaccactt aatctcagcc gaaaacttaa
    11581 agtattggag gaagaaatcg acaccaaagc caacctcata gtagtagtcg aacttttgta
    11641 gccttaacag aacacccttt tcaaccttga ggcgcttgta tgcggctaaa tcaattctaa
    11701 cgtcagttcc caggtacaag taggggcgga cattac
//
LOCUS       DAOOWJ010000081        11585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5547_length_11585_cov_7.766782, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000081 DAOOWJ010000000
VERSION     DAOOWJ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11585
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5547_length_11585_cov_7.766782"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1169)
                     /locus_tag="PLW22_08485"
     CDS             complement(99..1169)
                     /locus_tag="PLW22_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOU74780.1"
                     /translation="MKKFLIVGLMFSVITQLSAQNKEDILRNLENPDTLKQQSAQTSS
                     DKQPMVIVRKIVKVVDGDSIIEERIDTVTSVNALTVDSDNMAWVNTMGDTIKAAKGDT
                     VVVRLGKKKMVIVDSKDKTVIEIPEKGKKSDEIIEYKEVKKFKGHWSGFELGINGFMD
                     KNQSMTQTGDIAWMDLKQARSWNTNINFTQYSIGFGTDKAGLVTGLGLEFNDYHFSNP
                     ITLKVENGVTVVDSSYIQAGYKVEKTKITMSHLTLPLLLEFQIPMGEKRKDRIYISAG
                     VIGGVRLGSHTKVVYDDGSRHKDKNRGDFNIATLRYGLTARMGYKGIRLFANYYPVQL
                     FEKGKGPELYPFSVGLVLVPFD"
     gene            complement(1246..2283)
                     /locus_tag="PLW22_08490"
     CDS             complement(1246..2283)
                     /locus_tag="PLW22_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74781.1"
                     /translation="MTINRNNYELFIIDYIDGKLSPELTDELMVFLNQNPDIALEVDG
                     LAEIKLNPTPVKAPMAKESLKRSTSLTESGITEADYLCIAELENDLTAHESLQLNELK
                     KNHPDIARLSTLYSRTKLIANPAEIFPRKASLKHARITPTLSRIAYATVSIAAVLLIG
                     IYINSLLISRMDSNTLVAVQRPTTTPEQENNKPAETIELTKPIDNTVQETRRKPNLQY
                     SKPATTVRVAQVAQVTETTNRVSDEEIEPIATIEPQVTQTPQQEAQPNISINKSNSLT
                     TQGSISAKQVQPQTKELTLGDIALKGVQKLAQSVGINVDVKQTEGNQAKKIVVESRLL
                     AVSATILPKEE"
     gene            complement(2288..2773)
                     /locus_tag="PLW22_08495"
     CDS             complement(2288..2773)
                     /locus_tag="PLW22_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU74782.1"
                     /translation="MTAEEFNRCVDLYSDSIYRFALKMLKNTDLAMDNVQDCFERLWV
                     KHREVDFAKAKSYLFTSAYHAAIDSIRKEKRKLEYAGEAFFETSTSNGYSDAKEIVDK
                     AVEKLPEIQKSVLLLRDYEGYSYEEIGEITGLNESQVKVYIYRARLFLKEQLVSIDNL
                     I"
     gene            2919..4220
                     /gene="purD"
                     /locus_tag="PLW22_08500"
     CDS             2919..4220
                     /gene="purD"
                     /locus_tag="PLW22_08500"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOU74783.1"
                     /translation="MDKKLNVLLLGSGGREHALAWKISQSPLLEKLFIAPGNPGTGYH
                     GQNVAINPLNFDEVKGFCVQSAIDMVIVGPEEPLVQGIHDSFLSDSNVKHIPVIGPQK
                     LGAMLEGSKDFAKEFMFRHGIPTAQYKTFTAEKFNQAKDFLKTLNPPYVLKADGLAAG
                     KGVVIVPSYDEACANLQEFFAGKFGAASQKVVIEEFLSGIELSVFVLTDGHSYIILPE
                     AKDYKRIGDGDTGPNTGGMGAVSPVPFANQDFMKKVEERIVKPTINGLRNDAIPYKGF
                     IFIGLMNVYGDPYVIEYNVRMGDPETEVVMPRIKSDLLAALLACSQNRLSEASIEVDS
                     QTATTVIVVSGGYPNSYEKGKEIATPASIPSNAILFHAGTSISNGKLVTAGGRVFACS
                     ALGNGIDQALEKSYTLAKAIKFDKLYYRNDIGNDLKKLQNQ"
     gene            4222..5217
                     /locus_tag="PLW22_08505"
     CDS             4222..5217
                     /locus_tag="PLW22_08505"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOU74784.1"
                     /translation="MLLKSAQKSRFGALVFILLLLFATWLTTLMGKSPTYSFLFDSFP
                     MPFYQAVANLLSHYPIGYKLTAVILFFISGIYLLVLNNKFIILKNRSYLPILFYALCS
                     AAFLPIQRLNPALVSVFLVLLSIDHLFAIYQKNAPLDNLFRAGLAIGLATLLYAPAIT
                     IAVWALLTLLVLRPFNVREWFVLLFAFTVPWLIYMLILFIINDDILKPYHAFVTSIFT
                     ESEPAIDDKLFWIFGGFLAIPFGVSLIGTYQKLAFQKIIIRRYYSIFLYLFLIGVVSF
                     FLVPGVSYEIFYFTAIPSAFIMSSYFADAKQNFWNELLLWLPIIGAIVVQVVMLI"
     gene            5291..5824
                     /locus_tag="PLW22_08510"
     CDS             5291..5824
                     /locus_tag="PLW22_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU74785.1"
                     /translation="MKYAIVDIETSGGSWQRDRITEIAIYIHNGNSITNEFKTLVNPE
                     TIIPYYITQLTGITNGMVANAPKFYEIAREVVLLTQDCVFVAHNAPFDYRFIQAEFKR
                     LGYDYHREVLCTVKLSRRVFPGYGSYSLGNICNTLGIEIENRHRAAGDALATTKLFEK
                     ILFETNGDFTPFVKAKR"
     gene            5942..6220
                     /locus_tag="PLW22_08515"
     CDS             5942..6220
                     /locus_tag="PLW22_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74786.1"
                     /translation="MNIRKLYSHPYSAILAALLTASVFIFMFIYLAVTQRKHIYESSK
                     EVSKEVLRQTAGETQLYFTAAYVVANSIQQRAIILKQKMEPATILRIF"
     gene            6205..9774
                     /locus_tag="PLW22_08520"
     CDS             6205..9774
                     /locus_tag="PLW22_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU74787.1"
                     /translation="MKNLLKDVLTSNPNILGAWVLWESNAYDQNDSRFNHSNEFPCNG
                     RFGITYFRDNDTLYSEIVPCNDYKSFFYTDAKQKRSAVLSEPYLFRYTNYSNLYFGCT
                     ISVPIMSDTAFLGVVGVDINLSFLQNAISSVKLFDTGFLYLVSPKGLIVSYPDSAKIR
                     KNFYLEESRADSAYTAKAFMGNEVSAETYSNYLKERVFRFYYPINLYLSDKPWMVISE
                     IPIEKASSRSRQVVWVATFVMVIGILLIIYLVLNIFERRKYQQRIEDALCEAEKQKAN
                     AVESFQNYLEIFNSTSEAIFIYDAETGVIIDVNDQMLKMYGLHSKEEVIGKHVDVFSS
                     GIEPYDANGAQERIKQAIKAGSAVFEWQSKRSDGSIFWSEVSLKFTFISGKKTVLAVV
                     RDVSDRKIAQQALEYRERKFRELADLLPLVVWEVDLNARFTYANNIGFKLFGYTQEDL
                     DKGITIIDLIAPEDRQRVAQNIKKLFIESKTSDEEYLALRKDGSIFPANIFSSVVYEN
                     NIPKGLRGILIDVTEKKKAEQEIKEREQLYRTVVESLNEALMMVDNNDRILFVNTRFT
                     EMFGYKPEEVIGKVGNEFLIPEQRRHEIVEANNQRKQGIKNVYEMQFKAKDGRMVDCM
                     VSGAPVYNAQGAVTGSIGAIVDITELRKAEKAYHETMMLFETLSHMSPVGIFRTDAQG
                     KTTYVNPKWCELSGLTPEQAKGDDWIKAVHPEDRQKILDGWYERVNLEESSSTEYRFL
                     KPDGSVVWVLGNALPEKNADGINGYIGTITDITQIKQAQEKLERSERRFRELTELLPQ
                     SIWEIDLNGMVTFVNKYGLESMGYTYEDFKKGVNIFETIVPEFREETIRLFKRRLQGE
                     TAPGLEYPAQRRNGERFPALVYSSPIVDNGIVVGLRGISIDITEIKKTQQELETYRNH
                     LELLVKERTEELEAVNEELTSANEELYNQREKLIETLQELKNAQQTLIQSEKMASLGL
                     LAAGVAHEINNPLNFIQGGIIAIEGFINDKFKAQKSELQPLVDAIKEGVKRSAQIVRS
                     LNQYSRADYTKHSNVDLHRVIDNCLILLSNQYRDRIEIIKHYNEDSTSIYCNEGQIHQ
                     SFMNILINAIQAIDGTGKITITTKSTDKTCRVIFEDTGCGIDHENLTKIFDPFFTTKE
                     PGVGTGLGLSITYNIIKEHKGNINIHSERGKGTKVTVVLPKSS"
     gene            9777..10163
                     /locus_tag="PLW22_08525"
     CDS             9777..10163
                     /locus_tag="PLW22_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU74788.1"
                     /translation="MDATGNISVLHIDDEPTNVLLFEVNFRRYYQVISSNSGADGLTK
                     LTQNPNIDVIFCDMKMPGMNGLEFAREAAKVKPGIPCFILTGYDISPEIYHAINEGWV
                     SGYIQKPFEVDQIISAVNKATRTTAD"
     gene            complement(10160..11086)
                     /locus_tag="PLW22_08530"
     CDS             complement(10160..11086)
                     /locus_tag="PLW22_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOU74789.1"
                     /translation="MISFWKRDIGLHPTAEWEPYCWVNVENPTNEEKRYLLDELGVPD
                     AFYNDIEDVDERPRIEYENGWFFILMRLPYKNTDLKIPYTTVPLGIIFKDEVFVSISF
                     YRCEVIPDFIQFSVRKGILIKDHFDQVLRIMLSSSVWFLKYLKQINNDIKEAENQLER
                     SIRNEELQDLLRIEKSLVFFTTSLKGNDILLHRIKNLRSYRDTYNPELLEDVEIELRQ
                     AQETTSVYSDILSGMMDAYASVISNNLNIVMKRLTSISIVLMIPTLIASFYGMNVPNN
                     METHPWAFGAIVIASITISVFALLIFMRKKWY"
BASE COUNT         3308 a         2478 c         2480 g         3319 t
ORIGIN      
        1 ctcctgacag gagaggggta ggggtgaggt ctgtttacac catcaactac aaaaaggcgg
       61 gtaaaacccg ccttttgtaa cctaaccact aattgtaact aatcaaaagg aaccagaaca
      121 agacctaccg agaagggata tagctcaggg cctttaccct tttcaaatag ctgaactggg
      181 tagtagttgg caaaaagcct tatgcccttg taacccatgc gggcggttaa cccataccgt
      241 agggtagcaa tgttaaaatc gccgcggttt ttatccttat ggcgtgagcc atcgtcgtaa
      301 accaccttgg tgtgagagcc aagccttaca ccacctatta caccggcgga gatgtaaatt
      361 ctgtccttac gcttttcgcc catgggtatc tgaaactcga ggagtagcgg aagagttagg
      421 tgcgacatgg taatctttgt cttttccacc ttatagccgg cctgaatata ggagctatcc
      481 acaacggtta cgccattttc aaccttaagg gttatgggat tggagaaatg gtaatcgtta
      541 aactcaagac ccaaaccggt aacaagacct gccttatcgg ttccaaaacc aatgctgtac
      601 tgggtaaagt tgatgttggt gttccatgag cgcgcctgct ttaaatccat ccaggctata
      661 tcgccggttt gcgtcatgga ctggttcttg tccatgaatc cgtttatacc cagctcaaaa
      721 cccgaccaat gtcccttgaa ctttttcact tccttgtact caataatttc gtcggacttt
      781 ttaccctttt cagggatttc aatcactgtt ttgtccttgg aatcaacaat caccatcttt
      841 ttcttaccga gtcttactac cactgtatcg cccttggcgg ccttaatggt atcgcccatg
      901 gtgttaaccc aagccatgtt gtccgaatca accgttaaag cattgaccga ggtaacggtg
      961 tcgatacgtt cctcaataat tgaatcgcca tcaacaacct ttacaatttt tcgaacaatt
     1021 accatgggtt gcttatcgct tgaagtttgt gcgctttgct gtttgagtgt gtcgggattt
     1081 tccagattac gaagtatgtc ctccttgttc tgtgcgctaa gttgggtgat aacggaaaac
     1141 atcaatccca caattaaaaa ctttttcatt gttttatcag atttagttta acattcggtt
     1201 gttttaccat acgacgcccg aatgcaaaaa atgttacagg ggtagttatt cttcttttgg
     1261 taaaatagtt gccgaaacgg ccagcagcct cgattcaaca acaatttttt tggcctgatt
     1321 gccttcggtt tgctttacat caacatttat tccaaccgat tgggcaagct tttgtacgcc
     1381 tttaagggcg atatcgccaa gggtgagctc ctttgtttgg ggttgaacct gtttggccga
     1441 aatagaacct tgagtagtta agctgttgct tttatttatg ctgatattgg gttgtgcttc
     1501 ctgctgaggt gtttgggtaa cctgaggctc aatggtggct attggttcaa tttcttcgtc
     1561 actaacccta ttagtggttt cagtaacctg agcaacctgg gcaaccctaa ctgtagtggc
     1621 gggtttggaa tattgtaagt tgggtttacg ccttgtttcc tgaaccgtat tgtcaattgg
     1681 tttggtaagc tcaatggttt ctgcaggttt attattctcc tgttcagggg tggtggttgg
     1741 cctttgtact gccaccagcg tgttgctgtc catcctgctg ataagcaacg agtttatgta
     1801 gataccaatt agcagaacag ctgcaatgct tacggttgca taggcaatgc gcgataatgt
     1861 tggggttata cgggcatgct ttaggctggc tttcctggga aagatttctg ctgggtttgc
     1921 tattaatttg gttctactgt atagggttga aagtcttgcg atatcggggt gatttttttt
     1981 aagctcgttt agctgcaagg attcatgggc cgttaaatca ttctccagct cggcaatgca
     2041 aaggtaatcg gcctcggtaa tgccgctttc ggttaaactg gtcgatcgct ttaggctttc
     2101 cttagccatt ggtgctttaa ccggagttgg gttaagctta atttcagcta gcccatcaac
     2161 ctcaagtgca atatcagggt tttgattcag gaaaaccata agctcatcgg taagctcagg
     2221 gctaagctta ccatcgatgt aatcgatgat aaaaagttcg tagttatttc ggtttatagt
     2281 catagcccta aatcaagtta tcgatactta ccagctgctc cttcaggaac aaccttgccc
     2341 tatagatgta aaccttaacc tgcgactcgt taagccctgt aatttcgcca atctcctcgt
     2401 acgagtaccc ctcgtaatca cgaagcagga gtaccgattt ttggatttct gggagtttct
     2461 caacagcctt atcaactatc tccttggcat cggaataacc gttagaggtt gatgtttcaa
     2521 aaaatgcttc acctgcatac tctagcttcc gcttttcctt ccttatgcta tcaatggcag
     2581 catggtatgc ggaggtaaac aggtacgatt ttgcttttgc aaagtccacc tcacggtgtt
     2641 ttacccataa ccgctcaaag caatcctgaa cgttatccat tgccaaatcg gtattcttta
     2701 gcatctttag cgcaaagcgg taaatgctat cggagtataa gtcaacgcat ctgttaaact
     2761 cttcagcggt catgaaccgt tacttggtta tcaactttca aatgtatgac gattaagttt
     2821 catttaagtt acaacgttga gtaaaaaatt gttttgcgcc aatcactttc cattttaaaa
     2881 cgataactac ctttgtactc aaacacctgc cttgaacgat ggataagaaa ctaaatgtac
     2941 ttttacttgg ctctggcggc cgtgagcacg ccttggcatg gaaaatttcg caaagtcccc
     3001 ttttggaaaa actctttata gctccgggaa accccggtac tggctaccat ggccagaacg
     3061 tagctattaa ccctttaaat ttcgatgagg ttaagggctt ttgtgttcag agcgcaattg
     3121 atatggtgat tgtaggcccg gaagaacctc ttgtacaggg cattcacgat tccttcctgt
     3181 ccgatagtaa cgttaagcat attccggtaa ttggccctca aaaattaggt gctatgcttg
     3241 agggtagcaa ggattttgca aaggagttca tgttccgaca tggaatcccc accgctcagt
     3301 ataaaacctt tacagcggaa aagttcaacc aggccaaaga ttttcttaaa accctaaatc
     3361 ccccatacgt tctcaaggcc gatggacttg cagccggaaa gggagtggtt attgtgccat
     3421 cgtacgacga ggcatgcgca aacctccagg agttttttgc gggtaaattt ggagctgcaa
     3481 gccaaaaggt tgtgattgag gagttcctga gcggaataga gctatcggtc tttgtgctaa
     3541 ccgatggcca ctcgtacata atcctccctg aggcaaagga ctacaagcgt ataggcgacg
     3601 gcgataccgg gccaaatact ggaggtatgg gggctgtgtc gcctgtacca ttcgccaatc
     3661 aggattttat gaaaaaggtt gaggagcgta ttgtaaagcc taccataaat ggactcagga
     3721 acgatgctat cccttacaaa ggctttattt ttattggact aatgaacgta tatggcgatc
     3781 cttacgtaat tgagtacaat gttaggatgg gcgatccgga aaccgaggtg gtaatgcctc
     3841 gcattaaatc cgaccttctt gcagccctgc tggcttgctc acagaatcgg ttatcggaag
     3901 catccattga ggttgattcc caaaccgcaa caacggttat tgttgtatcg gggggctacc
     3961 caaattcata tgagaaaggg aaggaaatag ctacacctgc ttctattccc tctaatgcta
     4021 ttctattcca cgccggaacc tccataagca acggtaaact ggttactgct ggcggacgag
     4081 tttttgcctg ttcggcgctg ggcaacggca tcgaccaagc ccttgaaaaa tcgtacaccc
     4141 ttgctaaagc catcaaattc gataagctgt actaccgaaa cgacattggc aacgatttaa
     4201 aaaaactgca aaaccaatag tatgcttctc aaaagtgccc agaaaagtag atttggagca
     4261 ttagtgttca ttttgcttct cctgtttgca acttggctta ctacccttat gggtaaatcg
     4321 ccaacctact cattcctttt cgatagtttt ccaatgccgt tctaccaagc tgtagcaaat
     4381 ttgcttagcc attaccctat tgggtacaag ctaacagctg taatcctttt ctttatatca
     4441 ggaatttacc tgctggtgtt aaacaataag tttatcatat taaagaatcg gtcgtacctg
     4501 ccaattctgt tttatgcact atgctctgcg gcctttttac ctattcaacg gttaaaccca
     4561 gcgctggtat cagtattcct ggtgctgctc agcatcgatc atttatttgc catttaccag
     4621 aaaaacgccc ccctcgacaa cctttttagg gcagggttag caataggttt ggctaccttg
     4681 ctttatgctc ctgcaataac tattgcagtt tgggctctac ttactttgct agtactgcgc
     4741 cccttcaatg ttagggaatg gtttgttttg ctattcgcct ttaccgttcc ctggcttatt
     4801 tacatgctta tacttttcat tattaacgat gatattctta aaccttacca tgcttttgta
     4861 acaagtattt tcaccgaatc ggaacctgct atcgacgata agctattttg gatttttgga
     4921 ggttttctgg ctattccctt cggggtatcg cttataggta cctatcaaaa attggctttt
     4981 caaaaaatta tcattcgccg ctactactcc attttccttt acctttttct gattggggtt
     5041 gtttcgttct ttttagttcc aggtgtatcg tatgagatat tctactttac tgctataccc
     5101 tcagcattca tcatgtcgtc gtattttgct gatgctaaac agaacttttg gaacgaactt
     5161 ttgctgtggt taccaattat tggtgcgata gtggttcagg ttgtaatgct gatttgaaaa
     5221 aaaatttgtt agtattgggg tatatcgagt tcatatgcca ctggtagcaa tacccttaac
     5281 tgttatgtaa atgaaatatg ccatagttga tattgaaaca tcgggaggta gctggcagcg
     5341 cgataggata actgaaattg ccatttacat tcacaatggg aatagtataa ccaacgaatt
     5401 taaaacactg gttaaccctg aaaccataat tccctactac attacacagc taacgggtat
     5461 cacaaatgga atggtggcca atgcgccaaa gttctacgaa attgctcggg aggtggtact
     5521 gctcacccag gattgcgtat ttgtagccca caatgcacca ttcgattacc ggtttattca
     5581 ggctgagttt aaacgtttgg gctatgatta tcaccgcgaa gtgctctgta ccgttaagct
     5641 tagccgacgg gttttcccgg gatatggctc atacagcctg ggcaacattt gcaacacttt
     5701 aggaattgaa attgaaaatc gccatagggc tgctggcgat gcactggcta caacaaaact
     5761 ctttgagaaa attctttttg aaactaacgg tgattttacc ccttttgtta aagccaaaag
     5821 gtaacatttt aataccctgg tattagctaa aacgcttttg gtcaaaatta tcggctcaat
     5881 caaatttttt tccatataaa atactctatc tttacgtata ggaaactgta catgcgggta
     5941 aatgaatatt cgtaaactat actcacatcc ctattcagca atacttgcag ccttgcttac
     6001 ggccagcgtg tttattttca tgtttatcta tctggcagta actcagcgta aacacattta
     6061 tgaatcgtcc aaggaggtct caaaggaggt gcttcgtcaa actgctggcg aaacccagct
     6121 ctatttcacc gcagcctatg tagttgcaaa ttcaattcag caacgagcaa taatactgaa
     6181 gcaaaaaatg gaacccgcaa cgatattaag aatcttttaa aggacgttct taccagcaac
     6241 cctaatattt taggggcttg ggttctatgg gaatccaatg cttacgacca gaatgattcc
     6301 cggtttaatc atagcaatga attcccttgc aatggacgtt tcgggataac atactttcgc
     6361 gataatgata cattatactc tgagattgtt ccatgcaacg attacaaatc gttcttttat
     6421 accgatgcaa aacaaaagcg cagtgcggtt ctctctgaac catacctgtt caggtacacc
     6481 aactatagca atctgtattt tggatgtacc atttcagttc ccataatgag cgatacagcc
     6541 ttcctgggtg tagtgggggt tgacataaat cttagctttc tacagaatgc aatatcttca
     6601 gtaaaacttt tcgacaccgg atttttatac ttggtttccc ctaaagggct aattgttagc
     6661 tatcctgatt ctgctaaaat tcggaaaaat ttttacctcg aagaaagtag agccgatagt
     6721 gcctataccg ccaaggcctt tatgggaaat gaagtttctg ctgaaaccta ttcaaattac
     6781 ctaaaagaaa gagtatttcg gttttactat ccaattaacc tatacctgag cgataaaccc
     6841 tggatggtaa ttagcgaaat accaattgag aaagcatcgt cacgttcaag gcaagtggta
     6901 tgggtagcaa cttttgtaat ggttattggt atactactta ttatttacct cgttctcaac
     6961 atctttgagc gccgtaaata ccagcaacgt atcgaggatg cactctgtga ggcagagaaa
     7021 cagaaagcca atgcggttga aagtttccaa aactaccttg aaatcttcaa ctctaccagc
     7081 gaagctattt ttatttacga tgccgaaaca ggagtaatta tcgatgtaaa cgaccagatg
     7141 cttaaaatgt atggattaca tagtaaggaa gaggtaatag gtaagcatgt cgatgttttc
     7201 tcctcaggaa ttgaaccata cgatgctaat ggagcccagg aacgaataaa acaagcaata
     7261 aaggctggta gtgctgtttt tgaatggcaa agcaaacgat ccgatggctc aattttctgg
     7321 tctgaggtat cgcttaagtt cacttttatt agcggtaaaa aaacggtgct tgctgtagtc
     7381 cgcgatgtta gcgatcggaa aattgcccag caagcactgg agtaccgcga gcgtaagttc
     7441 cgtgaactag ccgacctttt acccctggtt gtttgggagg ttgacctaaa tgcgcgattt
     7501 acctatgcca acaacattgg cttcaaactt ttcggctata cccaggagga tctggacaag
     7561 ggcatcacca ttatcgacct cattgcccct gaagacaggc agagggttgc ccaaaatatt
     7621 aaaaaactct ttattgaaag taaaacatct gacgaggagt accttgccct cagaaaggat
     7681 ggttcaattt tcccagccaa catattctca agcgttgttt acgagaacaa tatccctaaa
     7741 gggcttagag ggattctaat tgatgtaacc gaaaagaaaa aggctgaaca agaaatcaag
     7801 gaaagggaac aactctatag aactgtagtt gaaagcctga atgaggcgct tatgatggtc
     7861 gataacaacg accgtatact atttgtgaac acccgattta cagagatgtt tggatataag
     7921 ccggaagagg taattgggaa agtaggaaat gagtttttaa ttcctgagca aaggcgacat
     7981 gaaatagttg aggcaaataa tcaacggaaa caggggataa agaatgttta cgaaatgcag
     8041 ttcaaggcca aagatggccg aatggtcgat tgcatggtta gcggtgcacc agtttacaat
     8101 gctcagggtg ctgtaactgg ttctattgga gcaatagtcg atatcaccga actccgaaaa
     8161 gccgaaaaag cataccatga aaccatgatg cttttcgaga ccctctccca catgtcgccc
     8221 gtaggcatct tccgaaccga cgctcagggt aaaaccacct atgttaatcc aaagtggtgc
     8281 gaactatccg gattaacacc cgaacaagca aaaggcgatg actggattaa ggctgttcac
     8341 ccggaagata ggcaaaagat actcgatggt tggtacgaaa gggttaactt agaggaaagc
     8401 tcatcaaccg aataccgatt cctaaaaccc gatggttcgg tggtttgggt tctgggcaat
     8461 gctttgcccg aaaaaaatgc cgatggaatc aatggttata taggaaccat caccgatatc
     8521 acccaaataa aacaagccca ggaaaagctt gaacggagcg agcggcgatt ccgtgaactt
     8581 accgaactcc taccccaatc catttgggaa attgacctaa acggaatggt aacctttgtg
     8641 aacaagtatg gactggaaag tatgggttac acctatgagg actttaaaaa aggggtgaat
     8701 atttttgaaa ccattgttcc tgaatttagg gaagaaacta tcaggctttt taaacgacgg
     8761 ctacaggggg agactgcacc tgggcttgag tacccagcac aacgcaggaa cggcgaacga
     8821 ttccctgctc ttgtgtactc atcccctata gttgataacg gaatagttgt tgggttaagg
     8881 gggatttcca tcgatattac agaaataaag aaaacccaac aggaacttga aacctataga
     8941 aaccacttgg aactacttgt aaaagaacgt actgaggaac ttgaggcagt aaacgaggag
     9001 ctaacctcag ccaacgagga actttacaat cagcgcgaaa agctaataga aaccttacaa
     9061 gaactaaaga acgctcagca aaccctaatc caatcggaga aaatggcatc gcttggtttg
     9121 cttgcggctg gggttgctca cgaaataaat aaccccctga atttcataca gggaggcatt
     9181 attgccattg agggatttat caacgataag tttaaagcac aaaaaagcga gctacagcca
     9241 ttagttgatg ctatcaagga aggagttaaa cgctcagccc agattgtaag aagccttaac
     9301 cagtatagcc gtgccgacta tacaaaacac tcaaatgttg atttacacag ggtgattgac
     9361 aactgtctga tactactttc caatcagtac cgcgatagaa ttgagatcat aaagcattac
     9421 aacgaggatt caacaagtat ttactgtaac gagggccaaa tacaccaatc gttcatgaat
     9481 attctcatca atgccattca agctatcgat ggcacaggga aaataaccat aacaacaaaa
     9541 tcaacggaca aaacctgtag ggtgattttt gaggataccg gatgcggaat tgaccacgaa
     9601 aatttgacaa agattttcga tccgttcttt accaccaagg agcctggagt gggaacaggg
     9661 ctggggttat ccatcaccta caacatcata aaggaacaca aagggaatat taacatacac
     9721 tccgaaaggg gcaaaggaac aaaagttaca gtagttttac ctaaatcgag ctagccatgg
     9781 atgcaacagg aaatatcagc gtgctccaca tcgacgatga gccaacaaac gtactgcttt
     9841 ttgaggtaaa cttccggagg tattaccagg taataagttc caactccggt gctgatgggt
     9901 taacaaaact tacccaaaac ccaaacatcg atgttatttt ctgcgacatg aaaatgcctg
     9961 gtatgaacgg gcttgaattt gcccgtgagg cagcaaaggt aaaaccggga ataccctgct
    10021 tcatcctgac aggctacgat atttcgcccg aaatatacca tgctattaac gagggatggg
    10081 taagcggtta catacagaaa ccgtttgagg ttgatcaaat tatttcagct gtaaataagg
    10141 ctacccgaac aaccgctgat tagtaccact ttttccgcat gaatatcagc aaagcaaaaa
    10201 cagatatagt tatcgatgca ataactatag caccaaatgc ccagggatga gtttccatgt
    10261 tattgggaac attcatccca taaaaactag caattagtgt tggaatcata agcacaatgg
    10321 agatagaggt taaacgcttc atcacaatgt ttaagttatt ggatataacc gaagcgtaag
    10381 catccatcat accgcttaga atgtcggagt aaacgcttgt agtttcctgt gcctggcgta
    10441 actcaatctc tacgtcctcc aacagttccg gattataggt atcgcggtag ctacgcaggt
    10501 tttttatcct gtgcagcagt atatcgttgc cctttagcga tgtggtaaag aacaccaagc
    10561 tcttctcaat gcgaagcaga tcctgtagtt cctcgttacg gatggaacgt tcaagctggt
    10621 tttcggcctc cttaatgtcg ttgtttatct gttttaggta cttcaggaac catacacttg
    10681 acgatagcat tatcctgagt acctgatcga aatgatcctt tattaaaatt cccttacgaa
    10741 ccgagaattg aatgaaatca gggatcacct cgcaacggta gaaactgatt gacacaaata
    10801 cctcatcctt aaaaataata ccaagaggga ctgtggtgta gggaattttc agatcggtat
    10861 tcttataagg taatcgcatc aggataaaaa accacccgtt ctcatactca atacgggggc
    10921 gctcgtcaac gtcctcaata tcgttataga aagcatcagg taccccgagc tcatcgagta
    10981 agtaacgttt ttcctcgtta gttggatttt caacgtttac ccagcagtag ggttcccatt
    11041 cggcagttgg atgtaaacca atgtcccttt tccagaatga aatcatgtac tatccctcca
    11101 aaattttata gaatagaggt atagtacatt acctctattc cgggttaact atcagggtga
    11161 taatcgtcca tattgaaatt tgactttttt tccgaccgtg caaagttaat tttttttgtt
    11221 ttaatactac catcaaattt gaatgtaatg ctttttttgt gaatattctt tagctgactt
    11281 ataaattaga accaatgctt tttagggata tttctagtgg tgagtcccaa tttattgggc
    11341 cttggaacga taaaaccttt ggcattcgaa ctccccctgt aatgctgagc ccagtcgaag
    11401 cttctctttg agcttacaac ttttaactta caaaattcct cgaccaatag gaatggctta
    11461 tgtagtggca taaaagcctg acccgacaat gggtcggggg tggaacgttg cggtgggctt
    11521 tgtaagcgat accacatgag tcacgccgca ggcgtggcga atagtatcgc gtggcagttc
    11581 caccc
//
LOCUS       DAOOWJ010000082        11373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5685_length_11373_cov_7.555222, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000082 DAOOWJ010000000
VERSION     DAOOWJ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11373
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5685_length_11373_cov_7.555222"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLW22_08535"
     CDS             <1..750
                     /locus_tag="PLW22_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74790.1"
                     /translation="RIMANPQLKLMGLHTHIGTYIMTTDAYRIAAAKLSELAASIAQK
                     FNHYIAYIDMGGGFASRNTLRGAYLSGEDTTPSFDEYADAITSAIINSQIKPDKLPTV
                     ILETGRALIDDAGSIAGTVLANKRLTDGRRALIVDVGVNLLFTSFWYDHKINPTQSHS
                     GLVEETVIYGPLCMNIDVLRPSIMFPPLKTGEHFLISRTGAYNNTQWLQFITTRPNVI
                     LIDTNRKTHIIRKAESIDSIVSNENIPDHLK"
     gene            770..2944
                     /locus_tag="PLW22_08540"
     CDS             770..2944
                     /locus_tag="PLW22_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOU74791.1"
                     /translation="MRAINGLNTIIKGLIRSYSQIFFSSEYSLAILLLLITFIDFSIG
                     IAGLVSALVATLTSYLIKLDRETTAKGLYGFNALLVGFGLGFYYDISITLLLLAAIAG
                     FFTLLVNITLQGVLGKYYLPYLSLPFVLSMWLVLSATWQLTSTEINQSSVYLLNRIFD
                     VGGFNFIAIHDWWLNHVKSNFINGYFISLGSIFFQFNVLAGVLVALTLMAYSRIAFSL
                     SVIGYSVAYIAYLTFGLDLTTLGYSYIGFNFILSAIAIGGYYYIPSTASYWWAVAITP
                     VIAMVTIGLAIIFKTLGLPILSLPFNIVVLMFIYSMRFRISVSGLREVMVQEGKPELN
                     LYSYQSFAKRFPNYGWIRIRLPFYGVWTVNQGHHGNYTHKGEWAHAWDFIVLDSDGKQ
                     FKSDGHLQTDYYCYGKNVIAPADGEVVEVDDGVDDNPVGEININKNWGNTVIIRHAAG
                     LYSKLSHLRKGSITVKVGDKVKFGTKVGEVGNSGRSAYPHLHFQIQATEYIGSKTIKY
                     PLSSYIVNDTELKTFDYPATDQNVCNIESDPLLNSMLNFKPGKLISWEVQHENITENI
                     TWEVGITYLNKLFIKCNKTNSKAFFELDDVYFYFTHFEGSKKSLLYKFYLAAYRIPLT
                     YFEGIKVSDSIPVNKWFGGIALYLHDFIAPFIQLLQAEYRVKVKRIGSEFDIEGFRFE
                     TTVEGRFLNRTVLTERFDLEIRANGKVEIIQEQNNTLAKCEL"
     gene            2932..4200
                     /locus_tag="PLW22_08545"
     CDS             2932..4200
                     /locus_tag="PLW22_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74792.1"
                     /translation="MRTIIVIVSVLVLTYRLGAQTDIASLDRLNYQYFLGKDYKNLKR
                     TAKEILNQGNDYYYLRMRLGILAYENQRYAYAYRNFTRAKQQNPFDTVTNEYLYYCYL
                     LAGRQADALQFLRTLSENDKNSHLKSLPMAKAPELILSYSYTGYDVKKYHTNPLNFEA
                     IENLAVLNAGLAFNFSERANGFFQYSNTSKVATFYSPSDPSGNYDTYKQNQFYFSYKS
                     QITQGFEFSGYTHWVFFTDVSNVSTFGRRSSSINLSTESLFGLNFTLIGWYLRGVINF
                     NYSNFANSNQFATEAYITYLPFSNLNLYSTVSGFYQLDKNWGGTYQSNFDVGFKVFKY
                     LWVESGMMIGNSFLYSRNLGRELNNSFIIPSKSFYGSLVIPAKKFFLRVGGHYSEINN
                     YSWDLVNYTKTSKLVLNAFGVNATLTIKFQ"
     gene            4203..4817
                     /locus_tag="PLW22_08550"
     CDS             4203..4817
                     /locus_tag="PLW22_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74793.1"
                     /translation="MKVKNLILLVLTTMSVNLAAQDFAKLSVAFTESYAKEKEGKYAD
                     AAKPLKAMYDEKSYEVNLRLGWLTYLQGQFSESVGYYSKAIELMPYAIEPRLGIVLPA
                     SAMGNWGSVVEQYNKILSIDPNNTLVLYRMGMISYENKDFKKAYTYFEKVVNLYPFDY
                     YSVLMLGWTNYQLGKTKEAKILFQKALLYDPTSASAKEGLSLLK"
     gene            complement(4837..5292)
                     /gene="smpB"
                     /locus_tag="PLW22_08555"
     CDS             complement(4837..5292)
                     /gene="smpB"
                     /locus_tag="PLW22_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOU74794.1"
                     /translation="MENKNNIVIKNKKATFQYEIIETFTAGIVLKGTEIKSIRMGKAS
                     FVDSYCHFIGEELWIKGLHIAEYAWGTYNNHDPKQERKLLLTRKELIKLKRRSEEKGL
                     TIIALKLFINQRGLAKVDIALARGKQLHDKREDLKHKDARREMDRAMKR"
     gene            complement(5276..5536)
                     /locus_tag="PLW22_08560"
     CDS             complement(5276..5536)
                     /locus_tag="PLW22_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74795.1"
                     /translation="MVKLRHPNPTNKLNELNRLVWFGLLLFVSGTAAILIATNFFSQP
                     LLIAKNYMLIAIIAILLMLYVYRLVGITRKPLKTPETHGKQE"
     gene            5599..6522
                     /gene="miaA"
                     /locus_tag="PLW22_08565"
     CDS             5599..6522
                     /gene="miaA"
                     /locus_tag="PLW22_08565"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU74796.1"
                     /translation="MLDSKYDLVAIVGPTASGKTQLAAHLAARVNGEIISADSRQVYR
                     GMTIGTGKDLDDYVVEGRLIPYHLIDIVDAGYKFNVFEYQQHFIDAYNDIKKRNRLPI
                     LCGGSGLYIDAVLRGYKLLPVPENPELRRELEAKTDEELATILASYKKLHNTTDLDTR
                     KRTIRAIEIEEYYQRQPVDPKPFPQIRSIIFGVKHQRAAEMKRIRERLAQRLNAGMID
                     EVKALLNAGLTPDDLIYYGLEYKYITEYLIGKHSYDEMFEKLYIAIRQFAKRQMTWFR
                     GMERKGFTINWIAGELTLEEKISLVLNKIKE"
     gene            complement(6512..6835)
                     /locus_tag="PLW22_08570"
     CDS             complement(6512..6835)
                     /locus_tag="PLW22_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74797.1"
                     /translation="MHRNRKQIYWFVVILTVFFIVFCSISLVNHYLFRTYALDLGMFN
                     QALRNVAQLQKPTFTLGIAGEEIPFLATHFSPIIFLFTPLYFTRVYSDNDLLIFKRKE
                     RKAIL"
     gene            complement(6922..9030)
                     /locus_tag="PLW22_08575"
     CDS             complement(6922..9030)
                     /locus_tag="PLW22_08575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU74798.1"
                     /translation="MKRIVKPICLAAMAIIIGGSGCTDTEKQVKEPLKIDVSLTQEEK
                     DKGLLTPEIMWKFGRIGSSVISPDGKLLAYTTTYYNLQENKGVTNIYLIPTEGGEPVM
                     LTDEQGSESNIQWSADGQAIRFLSTRSGSSQIWEVKIDGTGLKQLTNFDFDINGFAFS
                     PDGKKIVYAKDVKMVDQTEDIHPDMPKAKVRIIDDLMYRHWDYWEDGKFSHLFVADYT
                     ENGVENAIDINEGEKWDTPMATDFDMDEVQWSPSGDKIVYASKKLYGKDYAMSTNSDI
                     YLYDLKTRITTNLSDGNPGYDRYPRFSPNGVFISWWSMATPLYESDQKRLFILNTNNN
                     KKTYLTEGFDQNVEYYVWDNDSRNIYFTSGIKGTEQLYKVDVATSKITQLTTGKHDYT
                     SCQYSNGVLTASRMSMSMATELYRINEQTGEATQLTFTNKHIYDHIKMGEVRERWVTT
                     TDGKKMLVWVILPPDFDSTKKYPALLYCQGGPQSTVSQFWSYRWNFQIMAANGYVVVA
                     PNRRGVPSFGQEWNRQISGDYSGQNIKDYLSAIDDVKKEPWVNEDKLGCVGASYGGYS
                     VFYLAGHHQKRFKAFIAHCGMFNLESFYPQTEELFFPTFDLGGAPWETDNKVAQRSYA
                     NSPHKFVKNWDTPIMIIVGEHDYRIPYTQSLEAFGAAQLLGVPSKLLFFPNGTHFVTK
                     PQEAVVWQREFFGWLDKWLK"
     gene            9180..10793
                     /locus_tag="PLW22_08580"
     CDS             9180..10793
                     /locus_tag="PLW22_08580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOU74799.1"
                     /translation="MRKLFPLVLLLSVLSACNSSNKLNTNAISYDDVEHDIMVLSADS
                     MMGRAPFTVGEVRATAYIQNRMKDLGLVPAFGDTYFQDVPMVSITTHLPEQVDVGLAS
                     GKLSLKVDDDICLWSPTTRAKVEISKAPLLFLGFGINAPEYGCNDFRDIDVKGKIIVV
                     LVNDPGYYLLDSSLFTGYAMTYYGRWRYKFEEAERQGALGCIIVHEDGPAGYPWAVAG
                     SKSNSPILYVDSKELDTPACTLNGWITREAAEQLFSRCGYSYEAIKKQATSKDFKPIN
                     LNATLSLSLSNSVERSVSKNVAGYIKGAEKPDEFIVYTAHWDHLGVGRPLNGDSIYNG
                     ASDNAAAIAWMLSIAKVFKQADAPKRSIIFLAPTAEESGLFGSEYFVKHCPFDITKAI
                     ACINYDVVLFLGKFKDVTVTGYGHSNLDSLLQVVAEEHDRYIALDPNPENGMFYRSDQ
                     LPFLKAGVPAIFAKGYSHQVELGREKTLELIQDYWQNVYHKPCDEYNPQRDRLDGLVD
                     DAVLFYEVGNKLTNSNIYPQWRKASEFYR"
BASE COUNT         3187 a         2270 c         2421 g         3495 t
ORIGIN      
        1 agaataatgg caaatcccca gcttaagtta atgggtttgc acacccatat cggaacatat
       61 ataatgacaa ccgatgcata ccgcattgct gctgcaaagc tgtcggaact agcggccagt
      121 attgcgcaaa agtttaacca ttacatagca tatatcgata tgggtggtgg ttttgcatcg
      181 cgtaacaccc tgcgaggtgc atacctttct ggtgaagata caaccccctc gttcgatgag
      241 tatgctgatg ccataacctc agcaattatc aactcacaaa ttaaacccga taaattaccc
      301 actgtaattc ttgaaacagg gagagcattg attgacgatg caggaagtat agcaggtaca
      361 gttttagcaa ataaacggct taccgatgga cgcagggcct taattgtaga tgttggtgtt
      421 aacttacttt ttacatcgtt ttggtacgac cacaagataa atcccacaca gtctcattcg
      481 ggcttagttg aggaaaccgt aatatatggc cctctctgta tgaatattga tgtattgcgc
      541 ccatcaatta tgtttcctcc cttgaaaaca ggtgagcact ttttgatatc ccgaacgggt
      601 gcctacaata acacccagtg gttacagttt attaccacca ggcctaacgt tatactaatt
      661 gatacaaacc gaaaaacaca tattatccgt aaagctgaga gcattgatag cattgtttca
      721 aatgagaata ttccagatca cctaaagtaa accatcagca tacttgttta tgagagcaat
      781 aaacggatta aatacaatca ttaagggact aatcaggagt tactcgcaaa tctttttttc
      841 aagcgaatac tctttagcca ttcttttgtt actcataacg tttatcgatt tttcgattgg
      901 tattgctggg ttggtttcag cattagtagc cacacttaca tcttatctga taaaactaga
      961 tagggaaaca accgctaagg gcttatatgg ttttaatgct ttactggttg gatttgggtt
     1021 aggattttac tatgatatta gcattacact tctgcttttg gctgccattg ccggattttt
     1081 tacattactt gttaatatta cactacaggg agttttagga aagtactact taccttacct
     1141 atcgcttcca tttgtgttaa gcatgtggct agtgctaagt gctacttggc agctgacatc
     1201 aaccgaaata aatcagagta gtgtctacct actcaataga atttttgatg ttggcggttt
     1261 taattttatt gccattcacg actggtggct taaccatgta aaatccaatt tcattaatgg
     1321 ttattttata tcgttgggct ctatattctt tcagtttaat gtgctagccg gggttttggt
     1381 agcattgaca ttgatggcat actcacgaat tgcattttcg ttatcggtta ttgggtacag
     1441 cgtggcttat attgcctatt taacttttgg tctagatttg actacgttgg ggtatagcta
     1501 tattgggttt aactttattc ttagcgcaat tgccattggg gggtattact atataccctc
     1561 aacggcttcg tactggtggg ctgtggccat tacacctgta attgcaatgg taactattgg
     1621 gctagcaatt atttttaaaa cgcttggctt gccaatcctt tcgctaccct ttaatattgt
     1681 tgtacttatg tttatttact ccatgcgttt taggatttcg gtttctgggt taagggaagt
     1741 gatggtgcag gaagggaaac ccgaactaaa tctttactcc tatcaatcgt ttgctaaacg
     1801 ttttcccaat tatgggtgga ttaggattag gttacctttt tacggagttt ggaccgtaaa
     1861 tcaaggtcat catggcaatt atactcacaa aggggagtgg gcgcatgcat gggatttcat
     1921 tgttttagac tccgatggta aacagtttaa aagtgatggt catttacaaa ccgattatta
     1981 ttgttacggg aaaaatgtaa ttgctcctgc cgatggcgaa gtggttgagg ttgacgacgg
     2041 tgttgatgat aatcctgtag gtgaaataaa tattaataag aattggggaa ataccgtaat
     2101 aattaggcat gcagctggtt tgtattccaa gctttcgcac ctcagaaagg gttcaattac
     2161 cgttaaggtt ggcgataaag taaagtttgg aacaaaagta ggtgaggttg gcaattcagg
     2221 gcgttcagct tacccccacc tccattttca gattcaggca accgagtaca ttggctccaa
     2281 aaccattaaa tacccgttat catcatacat tgttaatgat actgaactca aaacattcga
     2341 ttatcccgct actgaccaaa atgtttgcaa tattgaatct gatccattgc ttaattctat
     2401 gctaaacttt aaacctggca agttgattag ctgggaagtt caacatgaaa atattactga
     2461 aaatataacg tgggaagtgg gtattaccta tttaaataag ctatttataa agtgcaataa
     2521 aacaaattct aaagcattct ttgagcttga tgatgtgtat ttctacttta cccattttga
     2581 gggaagtaaa aaatcactat tatacaaatt ttaccttgct gcatatagga tacccttaac
     2641 ctattttgaa gggataaagg tatctgattc aatacccgta aataaatggt ttggtggaat
     2701 agctctttac ctccatgatt ttattgcgcc attcattcaa ttattgcaag ctgaatacag
     2761 ggtaaaggtt aaaagaattg gaagcgaatt tgacatcgaa ggttttaggt tcgaaacaac
     2821 cgttgagggc agatttctaa atagaacagt tctaacagaa agatttgatt tagaaattcg
     2881 tgcaaacggt aaagtagaaa ttattcagga acaaaataat acccttgcga aatgcgaact
     2941 ataattgtta tagtttcagt tttggtgtta acctacaggc taggtgcaca aaccgatatt
     3001 gcatcgcttg ataggttaaa ctatcaatat ttccttggta aggattataa gaatttaaaa
     3061 aggactgcca aagaaatttt aaatcagggg aatgattact actacctaag gatgaggtta
     3121 ggtattttgg cctatgaaaa tcaaaggtat gcttatgcgt atcgaaattt tactcgcgct
     3181 aaacagcaaa atcctttcga tactgtaaca aacgaatatt tgtactattg ctaccttctg
     3241 gcaggcaggc aggctgatgc acttcaattt ctccgcaccc tttccgagaa cgataaaaat
     3301 agtcatttaa aatccttacc catggctaag gctcctgaac taatattaag ctattcatat
     3361 acaggatacg atgtcaaaaa atatcacacc aaccccttaa actttgaggc cattgaaaat
     3421 ctggctgtat taaatgcagg attagctttc aatttttcag aaagggctaa cggttttttc
     3481 cagtactcta ataccagtaa agtagctact ttttactcac cctccgatcc ctcaggcaat
     3541 tacgatacat acaaacaaaa tcaattttat tttagttaca aaagccaaat tactcaaggt
     3601 tttgagtttt cagggtatac ccactgggta ttttttaccg atgtatcgaa cgtttctact
     3661 tttgggcgca ggagttcttc cataaattta tcaaccgaat cgttatttgg attaaacttt
     3721 acattaatag gttggtatct gagaggggtt attaatttca actacagcaa ctttgcaaat
     3781 tcaaatcagt ttgctacgga ggcttacatc acctatttgc ctttttcaaa tttaaacctg
     3841 tattcaacgg ttagtggttt ttaccaattg gataaaaact ggggaggtac ttatcagtcg
     3901 aattttgatg ttggtttcaa agtatttaaa tatttatggg ttgaatccgg gatgatgatt
     3961 ggcaattctt tcttgtattc acgtaattta ggcagggaac tgaacaactc atttatcatt
     4021 ccttctaaaa gtttttatgg tagtttagtt atccctgcta aaaagttttt tttaagggtt
     4081 gggggtcatt actctgagat aaacaactat tcctgggatt tggtgaatta tactaaaaca
     4141 tcaaagctag tacttaatgc atttggagta aatgctacat taaccattaa atttcaatag
     4201 ctatgaaggt caaaaatcta atcttactgg tgctgacaac tatgtcggta aacttagcgg
     4261 ctcaagattt tgctaaacta tcagtagcat tcactgaaag ctatgctaag gaaaaggaag
     4321 gcaagtatgc cgatgctgca aaacccctaa aggccatgta cgatgaaaaa tcgtatgagg
     4381 ttaatttaag gttgggatgg ttaacatacc tgcaaggtca atttagcgaa tcggtaggct
     4441 actactcaaa agctatagag cttatgccct atgccattga accccggttg ggaattgtac
     4501 tccccgcatc agcaatgggt aattggggtt cagtagttga gcagtataac aagatacttt
     4561 ccattgaccc taataatact cttgtgttgt atcgtatggg tatgataagc tatgagaata
     4621 aggattttaa aaaggcttac acttactttg aaaaggtagt aaatctttac ccctttgact
     4681 attacagcgt actaatgctg ggttggacta attaccagtt agggaaaaca aaggaagcca
     4741 agatactttt ccaaaaggcc ttactttacg accctacaag cgcaagcgct aaggagggat
     4801 taagcttact gaaatagtat caattgtttt ggcgatttaa cgtttcatag cccggtccat
     4861 ttcgcgccgg gcatctttat gtttaaggtc ttcgcgcttg tcatgtaact gtttaccgcg
     4921 tgcaagggca atgtcaacct ttgcaagccc acgctggttg atgaatagct ttagggcaat
     4981 gatggttaac cctttctctt ccgaacgtcg tttcagctta ataagttcct ttcgggtgag
     5041 cagtagttta cgctcctgct tggggtcgtg gttattgtat gtaccccagg catactcagc
     5101 tatgtggagc cctttaatcc aaagctcttc gcctataaag tggcagtaag agtccacaaa
     5161 gctggcttta cccattctta tcgattttat ttcggtccct ttcagcacta ttcctgcggt
     5221 gaaggtttca ataatctcat actgaaacgt ggcctttttg ttttttataa ctatgttatt
     5281 cttgttttcc atgtgtttca ggggttttaa gtggtttacg ggtaattcct actagccggt
     5341 aaacgtaaag catcagcaga atagcaataa ttgcaattag catatagttt tttgcgatta
     5401 gtagtggctg gctaaagaag ttggttgcaa taagtatggc tgctgtaccc gaaacaaata
     5461 gtaataaacc aaaccatacc aaacggttta gctcattcag cttgtttgtc ggattagggt
     5521 ggcgtaactt taccattgct cacaaccttt aggtctgcaa atttagctat ttttacctgc
     5581 ttaactactg ttgtgtagat gttggatagt aaatatgatt tagttgccat agttggccca
     5641 acggcaagtg gtaaaaccca actcgctgct cacctggccg cccgggtaaa tggcgaaata
     5701 attagcgccg attcccgaca ggtttatagg ggtatgacca ttggaaccgg aaaggatttg
     5761 gacgattatg ttgttgaagg gaggttaatc ccttaccatc ttattgatat tgttgatgcg
     5821 ggctataagt ttaacgtttt tgagtatcaa cagcatttta ttgatgcata caacgatata
     5881 aaaaaacgca accgcctacc aatactttgt ggtgggagtg gtctctatat cgatgcggtg
     5941 ctgcgagggt ataagctatt gccagttcct gaaaatcctg aactccgacg agagctggaa
     6001 gctaagacag atgaagagct ggctaccatt cttgcatcgt acaaaaaatt gcataatacc
     6061 accgatttgg atacccgtaa aaggacaatc agggcgattg agatagagga gtactaccaa
     6121 cgtcaacctg ttgatcctaa gccattcccg caaattagga gcataatttt tggtgtgaaa
     6181 caccagcgtg ctgccgagat gaaacgtatc agggagcgtc ttgctcagcg cttaaatgct
     6241 ggcatgattg atgaggtgaa ggcgctcttg aatgcaggtc tcactcccga cgatcttatt
     6301 tactatggac ttgaatacaa gtatattacc gaatatctga ttggaaaaca ttcctacgac
     6361 gaaatgtttg aaaagcttta catagccata cggcaatttg ctaaaaggca aatgacctgg
     6421 ttccggggga tggaacgtaa ggggtttacc attaactgga tagctggtga gttgaccctt
     6481 gaggaaaaaa tatcgttagt tcttaataaa atcaaagaat agccttacgc tcttttcttt
     6541 tgaatatcag taaatcgttg tcggaataaa ccctggtaaa gtatagggga gtaaacagaa
     6601 aaataattgg agagaagtgc gttgcaagga aagggatttc ctctcctgct ataccaagag
     6661 taaatgtagg tttttgaagt tgtgcaacat tccttaaggc ctgattaaac atgcccaaat
     6721 ccagagcgta ggttcggaat aggtaatggt taactaacga tattgagcaa aatacgataa
     6781 agaataccgt gagaattact acaaaccaat aaatttgttt cctgttccgg tgcatttcaa
     6841 atatttacat gatgtaaata taaaaaaaaa cgggagtaac ccttaaggaa ctcccgtttt
     6901 attatggatt tgattcagta tttactttaa ccacttatcg agccagccaa aaaactcgcg
     6961 ttgccaaaca acggcttcct gtggttttgt aacaaaatgg gtgccgttgg ggaagaatag
     7021 cagtttactt ggtacgccga gtagttgcgc agccccaaat gcttcgaggc tttgggtgta
     7081 aggtatacgg taatcatgct cacccactat aatcataata ggagtatccc agtttttaac
     7141 aaacttatgg ggcgagttgg cgtagcttcg ctgtgctact ttgttatcgg tttcccatgg
     7201 cgcgccgcca aggtcgaagg tggggaagaa tagttcctcg gtttgggggt agaacgattc
     7261 caggttaaac atgccgcagt gggcaataaa cgctttaaag cgtttctggt ggtgtccggc
     7321 aaggtagaac actgagtaac caccgtagct tgcgcctaca caacccagct tatcctcatt
     7381 aacccaaggt tctttcttaa catcatcaat ggcgcttagg taatccttaa tgttttgtcc
     7441 gctgtaatcg cctgagattt ggcggttcca ttcctgccca aacgatggaa caccacggcg
     7501 gttaggagcc accacaacgt aaccattggc tgccataatc tggaagttcc agcgatagct
     7561 ccagaactgg cttacggtgc tctgcgggcc accctggcag taaagtaggg cgggatactt
     7621 ctttgtggag tcgaaatcgg gaggaaggat tacccaaaca agcatctttt tcccatcggt
     7681 tgtggtaacc cagcgttcac gaacttcgcc catcttaata tggtcgtaaa tatgcttatt
     7741 ggtaaaggtt agctgagtgg cctcgccggt ttgctcatta atccggtaaa gctcggtagc
     7801 catgctcatg ctcatgcggc ttgcagtaag tacgccgttg gagtactggc agctggtgta
     7861 gtcgtgcttg ccagtagtga gctgggtaat ttttgatgtg gcaacatcaa ccttgtagag
     7921 ctgctcagta cccttaatcc cactagtaaa gtaaatattt cggctatcgt tgtcccaaac
     7981 gtagtactca acattctggt caaaaccctc ggtaagatag gttttcttgt tgttattggt
     8041 gttgagaata aaaagtcttt tctgatccga ttcgtaaaga ggggtggcca tactccacca
     8101 gctgatgaag actccgttag gagagaatcg tgggtagcgg tcgtaaccgg gattgccatc
     8161 ggataggttt gtggtgattc gggtttttag atcgtacagg tagatatcgg agttggtgct
     8221 catggcgtag tccttcccat aaagtttctt tgaggcgtaa acaatcttat cgccgcttgg
     8281 gctccactgc acttcatcca tgtcgaaatc ggtagccatg ggggtatccc acttttcacc
     8341 ctcgttgata tcaatggcat tttctacccc attttcagtg taatcggcaa caaacaggtg
     8401 gctaaacttg ccatcttccc agtaatccca gtggcgatac atcaaatcat caattatccg
     8461 taccttggcc tttggcatgt ccggatgtat atcctcggtt tggtcaacca tcttcacatc
     8521 cttggcgtac actatttttt tgccatcggg cgaaaaggca aagccgttaa tatcaaaatc
     8581 gaagttagtg agctgtttga ggccggttcc atcgattttt acttcccaaa tttgcgagga
     8641 accactacgg gtggacagga agcgtatggc ttggccatcg gcactccact gaatattcga
     8701 ttccgagcct tgctcatcgg tcagcattac tggctctcca ccctcggtag gaattaggta
     8761 gatatttgtt actcccttat tctcctgtag gttgtagtag gttgtggtgt aggcaagtag
     8821 tttgccatcg ggcgagatga ccgaactccc aatacgtccg aatttccaca tgatctcagg
     8881 tgtgagaagg cccttgtcct tttcctcctg ggttaacgat acgtcaatct ttagaggttc
     8941 cttaacttgt ttttcggtat cggtacagcc cgaaccgcca ataattattg ccattgcagc
     9001 aagacagatt ggttttacaa ttcgtttcat ggtgctttgg gttaggttac acaattttga
     9061 ccctaaagtt agcatttggt taaatatcaa cctatgattt ttactttact tcttttttta
     9121 gtcaaattaa aagactaggg ataaattaat ttttactttt gttcaaaact actaatgcca
     9181 tgcgaaaact atttcctttg gtactgcttc tatctgtcct atctgcctgc aatagcagca
     9241 ataagctgaa cacaaatgcc atatcctacg acgatgtgga acacgatata atggtgcttt
     9301 cggccgatag catgatggga cgtgcaccat ttaccgtggg cgaagtaaga gcgaccgcat
     9361 acattcaaaa caggatgaag gacttagggc ttgtgcccgc ttttggtgat acatattttc
     9421 aggatgttcc catggtttcc attacaactc atttacccga acaggttgat gtaggtttgg
     9481 caagcggtaa gcttagcctt aaggttgatg acgatatctg cctgtggagt ccaaccactc
     9541 gggcaaaggt tgaaatatcc aaagcaccct tgctattttt gggttttgga ataaacgcac
     9601 cagagtatgg ctgtaacgat ttcagggata tagatgtaaa gggcaagatc attgtggttt
     9661 tggtgaacga ccccggttac tacctgcttg actcatcgct ttttactggc tatgccatga
     9721 cctattacgg acgctggcgc tataagtttg aggaagccga acgccaagga gcgcttggct
     9781 gcataattgt tcatgaggat ggtcctgcag gttacccatg ggcggttgcg ggtagcaaga
     9841 gcaacagccc aatactttac gttgatagta aagaactcga tactccggcg tgcactctta
     9901 atggctggat aacccgtgag gccgccgagc aactattcag tcgctgtggc tatagctacg
     9961 aggcgataaa gaaacaggct accagtaagg attttaaacc tataaaccta aacgcaacat
    10021 tgtctctaag cttaagtaat agtgtagagc gttcagtatc aaagaatgtc gcaggttaca
    10081 ttaagggggc tgagaaaccc gatgagttta ttgtatatac cgctcactgg gatcaccttg
    10141 gggttgggcg cccactaaat ggcgattcca tctacaatgg agcatcggat aatgctgccg
    10201 caatagcatg gatgctatcc attgctaagg tatttaagca agccgatgca cccaaacgct
    10261 caattatctt ccttgcgcct accgctgagg agtcgggact gtttggctcg gagtactttg
    10321 ttaagcattg cccgttcgat ataacaaagg ctatagcctg cataaactac gatgtggttc
    10381 ttttcctcgg aaagttcaag gatgtaaccg taaccggtta cggacactcg aatctcgata
    10441 gtcttttaca ggtggttgca gaggaacacg accgctacat tgcccttgac ccaaatcccg
    10501 aaaatggaat gttttaccgg tccgaccagt taccatttct taaagccggt gttcccgcaa
    10561 tttttgcgaa agggtattcg catcaggttg agttggggcg ggaaaaaact cttgagctta
    10621 ttcaggatta ctggcaaaac gtttaccata aaccttgcga cgagtataat ccgcagcgcg
    10681 ataggttaga tggtttggtt gacgatgcgg ttctgttcta cgaagttggt aacaagttga
    10741 cgaatagtaa tatctatccg caatggcgca aagcatcgga gttttacagg taacctgccg
    10801 acgtttaatc gagattcgtc aattgaattt tcaattgacg aatccctcta aaaatattgc
    10861 tgcgttccaa tttttttgcc cggttaagtt agttaatcta tggtatttag aattaactgc
    10921 tcacattacc tctaacttct tctaacatca tcttattccg tctattccga ctcattccgg
    10981 cacaaaacag acttttaact tttaactttt aacggttcac ggttcacgaa cataaagctc
    11041 tccaaccccc tcatatcctc aattcttctt ttaactttta acttttcact agtcctaact
    11101 ccctctaact tcctctaact ccgtcatatt cttaattctt ccggttcacg gttcacggtt
    11161 cacgtttaac ttcctctaac ttcctctaac ttcctcatat tccagcttat tctggctcat
    11221 tccgactcat acctcaagaa attaacccgg ttgaatttgc tagttcattg catttgggtt
    11281 taaatattaa tgttattttt atgccgggat tcgatgtttc aattcgcgtt ttcaattttt
    11341 actatttgtt taaacaacag taactaaacc tta
//
LOCUS       DAOOWJ010000083        11362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5696_length_11362_cov_8.121429, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000083 DAOOWJ010000000
VERSION     DAOOWJ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5696_length_11362_cov_8.121429"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..717)
                     /locus_tag="PLW22_08585"
     CDS             complement(193..717)
                     /locus_tag="PLW22_08585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU74800.1"
                     /translation="MIKPYKLKYSIQSVNPLLVIIITTITLLLIESFYHYLFLAFDIH
                     HTSNLPKLPVETDPESHVKIAIRLVKVCIIAPLVETFIFQYLLFELFWRKLRWSLNVF
                     YGLSAFTFGLSHFYSIPTIIVTFTVCLVFNYVYAALAEKKLNLNAYFVIVAIHSLYNT
                     SVFVNHIFSYLGSL"
     gene            complement(770..970)
                     /locus_tag="PLW22_08590"
     CDS             complement(770..970)
                     /locus_tag="PLW22_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74801.1"
                     /translation="MVNFESFSVHEMNTSEMTDINGGFSWEDYLIGKGIDAALECMKE
                     LFIAYVNYSASTGGKYVIHHAQ"
     gene            complement(1954..5292)
                     /locus_tag="PLW22_08595"
     CDS             complement(1954..5292)
                     /locus_tag="PLW22_08595"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74802.1"
                     /translation="MKLKNNSGLEFEFHSNGGIKRISSNGIRIGLRDTNPFSMFGTSI
                     FLRERNTPKKYIMLFGTGSNSVFTFEDNCYFAKGKWNGIEYLISLQLHNTDSCWLWSI
                     ELTNGNSSPSNYDIIIIQDVGLKQLSVGPVNEYYVSQYTERLILDDPQYGAVVCCRQN
                     MKEPTGNPWLMAACKNGAASATTDGLYVYGNSYRKTKIPLALELDTLPGECAAESSVI
                     AIQSIPFDFSSGNNQQISFVFRFLPDHPSATSSTDLAILPNLFEQFKSKYPTTSNWEK
                     GKHNLFISSEFFPSQDLSENEINHFYSSEKRFPDFSNGKLLSFFYGKGKHVVLGEKDI
                     LAYRPHGHILQSNINLNQDDNIVSLTTWSFGVFNSHLKQGNTNFNTLLSINTSQFNLI
                     PETGQRIFVEINNRYYQLGVPSAYEMGLNYARWIYKLDSNIFQVTTWASPNTPVVNFD
                     FKVISGHPVNIIITNNLDELNGWQVKSSGFPNGLTFIPSPESLLSKNYPKAQYRLVIN
                     KSDCANLKFDNGNCIDIESSYANSFVVIKSDSTKQLSISFICELQNLFDQSTINNPDQ
                     QFQADLEDALAFREKSGLEITLSGNSDKIEIINEIIPWFESNALIHYLTPYGLEQFGG
                     AAWGTRDVSQGPVEMLLSLGKYNEVKKILQTIFSNQNPDGWWPQWWMFDRYKTIRAHE
                     AHGDIAYWCIIALSNYIINSGDVDILNSPLPYYTENNGDSREVTPLIEHVERLIDRIV
                     KSFIPGTALVPFGGGDWNDSLQPVNSQLAQRLVSSWTVEMNYQAFKLFETVYRLMGLT
                     DKAEALGEISTKIKTDFNKYLVTNGVVAGYGHLENDNTFTLLLHPSDSKTGVSYSILP
                     MNRGILSGIFDLNQAFNHQKIITNHLLGPDGARLMDKPLKYSGGLQKIFQRAESSTFF
                     GREIGLMYTHEHIRYAETLAVTGKAEELIRAILQAVPISYTNIVKQGDIRQSNCYYSS
                     SDVLFKNRYEADKLYPEVIKGNIKLHGGWRVYSSGPGIFIGIILGRLLGFRFSNEFVV
                     IDPVLPKEFDGLRVNFSFSNIPFSVTYHITKSTFSPLSLKVNHVEPPFTYEENPYRKG
                     GIRVERNLFFGLLNSKENRIEIVL"
     gene            complement(5289..7727)
                     /locus_tag="PLW22_08600"
     CDS             complement(5289..7727)
                     /locus_tag="PLW22_08600"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOU74803.1"
                     /translation="MNKFGIEGGYFSLNGRKVFLNSGEIHYFRIKRELWEEHLKKAKE
                     AGLKTISSYVPWVWHEVNEGEFDFSGTTQPERDLIGWIELCQKHGFTCIVKPGPFILA
                     EYRGAGLPDWFLERYGDTIKMHNSKGEAVLSDGVSLFSPAFLSKVEQWYNHVMPVICQ
                     KQIQNGGPIIMVQVCNEIGVFSWLAHQADYNEQVKEQFISFIKTRYSTIDELNTLWET
                     NYTSFQEIELPPDGNTPYKSKGDRSRDYEWHCFWRKYYANYLRLLSSWIRKHNISIPL
                     YHNLPGWIYGHGYEFPLNITMYEDLYKDKSELIFGVDHIPEFVSYRNLHDDRIINDIV
                     SAMQGEKPLFAAEFQSGSREYHVVTNPREMELFYKASIANGLKGWNYYMFSQGKNPPA
                     KGYSGDTFYWFTPLDNQAKPSSAFPVVQKMSRFIETHEELILTTKRKAEVCVLFYSPY
                     YATELERPEVGASELKFVPARIRRSAYFDGLLKALQVLNVDYDMVDLASSSVNDLLAY
                     KQVWLFSTDEMNAPDQQTIASFVTNGGNLVIFPYLPDREMNQKPCTVLRDTFKIKSSQ
                     PEIIDSPLIKLYNLTDIKCSNPLIAYTTEAIDGATPIAFTLRGSICGFEKNVGKGKVV
                     HLGTWLGFDTEGHLQAYTTILERSKAQLANASSTNNFITVRQKFNDTSESLLFVGNYY
                     NENQSGKITYKHPNTNERVQLPLIDGEIEIPPLYAFISPISKEIGKGINILHTTSDVI
                     GINSNKNIITIHLTGDPGLIGEMVLEGINITKIKSAEISEKPIMLIWHNNRIVLNYLH
                     SNKEILLTLKMLDK"
     gene            complement(7742..9745)
                     /locus_tag="PLW22_08605"
     CDS             complement(7742..9745)
                     /locus_tag="PLW22_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU74804.1"
                     /translation="MENNNLQAKSEYRIKISQLLAYGAGGIIPIALFNIAGILVGLMG
                     NISLGLSAFWLGTILIIPRLWDAIADPIIGHLSDNTRTRWGRRRPFLLVGGILVALFF
                     VAMWWIPKGETIRIWFPSESGYQWFQLSYILVTLLLFYTSCAIFEIPHGALGMEMTVD
                     PHERTRLFSAKSFVGNLFAMSTPWLFVLANLETFKGPGGNEADGMRYVSLMIAALLIP
                     LSFWWTAKLREPSFVRVQKQEKKPFWTDMKHTTKNRNFIRLTLAIFTLATGFNFVTLL
                     GSYIPIFYVFSGDKVAGAYLLGINGTIWAVTGLLAVFPLNWISPKLGKRNTLILSIAL
                     MATAQLSKIVCYNPEYPYLIIIPTILLSAGMLFFFTLGSSMVGDICDEDDLNTGHRKE
                     GSFYSVFWWFIKMGFAVASFVAGILIEFTQFDQNQVTKVDYLEGSIKEIKVNLTDKNV
                     LNYTDYIKKIKDINTDILGNAIKYDAFLERVNQHENPQTDSLKIQAKVALRQNLSITI
                     DSLKYWDSVLNTSSPDSVNLSIIAQKLNKQRMNFNLIKAQNNVYDLMNHFESIIAGKG
                     DGNHYKELLRDIAQINCVLQEYNIYDIDSVNIHKLDEITQKIPSLKRQSPFTLLMMRV
                     VEIGLPVIMSILSLIFIFRYNLTEKRLQEIKQTLTTRNKLQNQ"
     gene            complement(9758..>11362)
                     /locus_tag="PLW22_08610"
     CDS             complement(9758..>11362)
                     /locus_tag="PLW22_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74805.1"
                     /translation="VMAETDNLGLNGGFVKYTIKLKAGQSHTLFAIAPYHSKNINDSE
                     LKTKNIVDTIAKVSDYWRGKTEYVKFNLPSTAEHLLKTYRANLMYILINRDIHGIQPG
                     SRSYERSWMRDGSLTSSALLKSGITDEVKEFIEWYAQNLYENGKVPCVVDFRGPDPVA
                     EHDSHGQFIYLIHEYFSFTHDTAFLRKMNPYILRTIGYIESLIAERSTDYFRYGNDSL
                     RAFYGLVPESISHEGYSAKPMHSYWDNFFILKGLKDACDIQIALGNHEKYTRIKQVTD
                     TFRKNLYKSINLTIKNHNINYIPGCAELGDFDATSTTIALTPCNEFENLPKPEIYNTF
                     ERYYTYFSQRKTGQINWVNFTPYENRIIGSFIMLNQPERAHELIDYFLSQQRPQGWYH
                     WAEVVWNDYRTPAYIGDMPHTWVGSDFINAFRNIFVYEDIDHNTLCIGSALKQDWIDS
                     PQGISVQNLPTYYGNLSYSITKGNDHYKIKIGGNIRVPANGICIRNFNGSKLPKGVEV
                     NGTKLNTYNVNEIRVNEIPCEIKIYY"
BASE COUNT         3304 a         2174 c         2295 g         3589 t
ORIGIN      
        1 acaaggagag cagaaactca aacgaagcca aactgaacag agttaacttg caaaaaaggg
       61 ttgttcaaaa ggaaagagca agaaagtaat ttgaatatga tgcttgctga tctatagaaa
      121 cattgaagaa ataataactc tttgcaatgg gttgtcttta aattatgctt tcattgtcaa
      181 aatatttttg gatcaaaggc tgccaagata actaaaaata tgattaacaa aaactgatgt
      241 attgtatagc gaatgtatag caacaataac aaagtatgca tttaaattta gctttttctc
      301 agctaatgcc gcataaacat aattaaacac taagcaaact gtaaatgtta caattattgt
      361 tggaattgaa tagaagtgcg agagtccaaa ggtaaaggca ctcaaaccgt aaaacacatt
      421 caagctccaa cgtaatttgc gccagaacaa ttcaaatagc aagtattgga aaataaaagt
      481 ttctactaaa ggagcaataa tacatacctt aacaagacga atagcaattt tgacatgaga
      541 ctcaggatcg gtttcaactg gtagctttgg caggttgctt gtatggtgaa tgtcaaatgc
      601 taagaataaa tagtgataga atgattcaat taataataaa gttattgtcg tgataataat
      661 aaccaataaa ggattaactg attggatact gtattttaat ttatatggtt taatcatatt
      721 aaaaaaatta caaaaaatag ggttataata tttataaccc tattaataac tattgtgcat
      781 gatgtataac atattttcca cctgtactag ctgagtagtt gacatatgct ataaataatt
      841 ctttcataca ctctagagca gcatcaattc ccttgccaat taaataatcc tcccaactaa
      901 aaccaccgtt gatatcagtc atttcacttg tattcatttc gtgaacagag aatgattcaa
      961 aattaaccat agcaaaaata ctttttattg ttaaataatc aaaccctctt tctttatcct
     1021 gccgaggtta gcagtgtaac gttacagttg caaaattagg agtgttccgt acggaaaaca
     1081 agaacggaag tgaaacaccc ccattttttt ctactaattc ttttcggctt aggggtttct
     1141 tggaaaaact taatgaaatg tcaaaggtgt attcatagaa aaaagtttca cgtgacttgg
     1201 agtacacaat gggtgcacct cgcgatctta gttcctcaat catttcgtaa agcctagtac
     1261 ggcttattcc tagtttggtc gcaaactcct caggagtgcc ggttcgctgt tccttaacta
     1321 gtcgtccctt aaaaagtcag tttaaaattt tttcttgaga ataaaaaaac atggaaaggg
     1381 gaaaagagtg catgtaaaaa gaaaaatagt gtttggaaga aatggtaaat atttttcttc
     1441 cacctgttca taaccctttt gaagttaaat gctgcggctg ccagcatgat actgatgtta
     1501 tcgcctacta ttccccccgc tcgcgcggat ttaccttcgg tgagctccct tcggtcggtt
     1561 gtaatccgtg cggtttagtt cggcaaagcc aatccgtgcg gttatttcat aatgcaaagt
     1621 tcagcaaagg ccgctcgcgc ggatttgcaa tcccgctcgc gcggatttac cttcggtgag
     1681 ctcccttcgg tcggttgtaa tccgtgcgtt tgtagttcgg caaagccaat ccgtgcggtt
     1741 attttataag gtggtttcaa tgaggcaaag ctcgaataag taaaagtgta gtatgtattt
     1801 gtcacggact acaagtccgc gccagcaatg agtggcgtat gccgcacgcg tttaaaaacg
     1861 cgcgcgagcg aatgtagcga aaagtatgcc gtacacgtcc agaggcaggt gaatgggggt
     1921 tatggtaaat cccttgtctt aaacaatatt tttctaaagt acgatctcga tcctgttttc
     1981 cttgctgttc agcaaaccaa aaaataagtt tctttcaact cttattccgc cttttcggta
     2041 tgggttttcc tcgtaggtaa agggcggttc tacatgattt actttaagag agaggggact
     2101 aaaggttgat ttggtaatgt ggtaggtaac actaaatggt atgttactaa agctaaagtt
     2161 tacccttaag ccatcaaatt cttttggtag aactgggtca ataacaacga actcattgct
     2221 gaatcggaat ccgagtaacc tgcccaagat aatacctatg aaaatgccgg gtccactgga
     2281 gtaaactcgc cacccaccat gtaatttgat gtttccttta ataacctcgg ggtatagctt
     2341 atcggcctcg tacctatttt taaatagcac atcagagctg ctgtaatagc agtttgattg
     2401 tcgaatatcg ccctgtttta ctatgttagt gtaactaatt ggtacggctt gcagaatggc
     2461 acgaataagt tcttcggctt tgccagttac ggcaagagtt tccgcatatc taatatgttc
     2521 gtgagtgtac attaagccaa tttcccttcc aaaaaatgta ctgctttcgg cacgttggaa
     2581 aattttttgt aaacctccgc tatacttaag gggcttatcc ataagtcggg caccatcggg
     2641 gcctaaaagg tgatttgtta ttattttttg gtgattgaaa gcctgattta aatcaaaaat
     2701 cccgcttaga atacccctgt tcattgggag gatgctataa ctaactcctg ttttactatc
     2761 ggagggatga agaaggagtg taaaagtgtt atcgttttct aaatgtccgt acccggctac
     2821 aacaccattg gtaactaaat atttattaaa gtctgtttta attttagtac ttatttcacc
     2881 taatgcttcg gctttatcgg ttaaccccat taacctatag actgtttcga acagcttaaa
     2941 ggcctgataa ttcatttcaa ctgtccagct cgataccaaa cgttgtgcca attggctgtt
     3001 aaccggttga agcgaatcgt tccaatcgcc acccccaaat ggcactagtg cagtgccagg
     3061 aatgaacgat ttaacaatcc tatcaattaa ccgttcaaca tgttcaatta gtggagtaac
     3121 ctccctgctg tcaccattat tttcggtgta gtagggtaaa ggtgagttca gaatatcaac
     3181 atctccgcta tttatgatgt agttggagag ggctataata caccagtagg caatatcgcc
     3241 atgggcctcg tgagccctga tggttttata cctatcaaac atccaccatt gtggccacca
     3301 cccatcgggg ttctggttgg aaaagattgt ttgaagaatt ttttttacct cattgtactt
     3361 acctaaactt aacaacattt caacaggacc ctgggaaaca tcgcgtgtac cccatgctgc
     3421 acctccaaat tgttctaacc catagggagt gaggtaatgg attagagcgt ttgactcaaa
     3481 ccagggtata atctcgttaa tgatttcaat tttatcggaa tttcctgaaa gggttatttc
     3541 taatcccgat ttttctctaa atgccagagc atcttctaaa tctgcctgga actgttggtc
     3601 ggggttgttg atggttgact ggtcaaaaag gttttgaagc tcacaaataa aacttatgct
     3661 taactgtttt gttgaatcgg atttgataac gacaaacgaa ttggcatagc tgctttcaat
     3721 atcaatacaa ttgccgttat caaattttaa attagcacaa tccgatttgt ttataaccaa
     3781 acggtattgg gctttagggt aatttttaga aagaagactt tctgggctag ggataaaggt
     3841 taaaccgttc ggaaagcctg acgattttac ttgccaacca ttcagctcat cgaggttatt
     3901 ggtaatgata atgtttactg gatggccaga tattacctta aaatcaaaat ttacaactgg
     3961 tgtattaggt gaagcccatg ttgttacttg aaaaatatta ctatcgagct tataaatcca
     4021 tcgagcataa ttcaatccca tttcgtaggc cgatggtaca cctagctggt aatacctatt
     4081 gttgatttct acgaaaattc tttgacctgt ttcgggtata aggttgaatt gacttgtatt
     4141 tattgaaagt agtgtattga aattagtgtt tccttgtttt agatgagaat taaaaacacc
     4201 aaacgaccat gttgtaaggg aaacaatatt atcatcctga tttaggttga tattggactg
     4261 gagaatgtgc ccgtgaggac gataggccag aatatctttt tctccaagta caacatgctt
     4321 gcccttgcca taaaaaaaag aaagcaattt accgttacta aagtcaggga atcgtttctc
     4381 agaactataa aaatggttaa tttcattttc actcaaatcc tgggatggga aaaattcaga
     4441 gctaataaaa agattatgct ttcccttttc ccaattgctt gtggtagggt atttgctttt
     4501 gaattgttca aaaaggttag gtagaatggc taaatcggtt gatgaggttg ctgagggatg
     4561 atcgggcaaa aacctaaaga cgaaactgat ttgttggtta ttaccactgc tgaaatcgaa
     4621 tggtattgat tggatagcaa taactgacga ttcagcagca cattcgccgg gcaatgtatc
     4681 gagttctaaa gcaaggggga ttttagtttt tcggtatgag ttgccgtaaa cataaagccc
     4741 atcggtagtg gccgaggctg caccattttt acatgccgcc attaaccaag ggttaccggt
     4801 aggctctttc atgttttgcc ggcagcaaac cacagcacca tactgaggat catccaggat
     4861 tagcctttca gtgtattgac ttacgtagta ttcattcact gggccaactg atagctgctt
     4921 taagccaaca tcctgaatta ttattatgtc gtaattggaa ggtgaagaat tcccattggt
     4981 taattcaatt gaccatagcc agcaactatc tgtgttatga agctgaaggc tgattaagta
     5041 ttcaataccg ttccacttcc cttttgcaaa gtaacagtta tcctcaaaag taaaaacaga
     5101 attactgcct gtcccgaaaa gcattatgta tttcttgggg gtatttcttt ctctaagaaa
     5161 tatacttgtc ccaaacattg agaatgggtt ggtatcgcgt aaacctatac gaataccatt
     5221 agatgaaata cgctttattc cgccattact gtgaaattca aattctaaac ctgaattgtt
     5281 tttcagcttc atttgtcaag catttttagt gttaacaaaa tttccttgtt gctgtgtaag
     5341 tagttcaata ctatccgatt gttatgccaa atgagcataa tgggtttttc ggagatttcg
     5401 gccgacttaa ttttggtgat gtttatccct tcaagaacca tttcaccaat caatcctggg
     5461 tcgcccgtaa ggtgtatagt aatgatattt ttgtttgagt taatcccaat aacatcggag
     5521 gtagtatgaa gtatattaat gcctttacca atttctttac taattggtga aataaaggcg
     5581 taaagcggtg gaatctcaat ttctccatcg attagtggga gttgaaccct ttcgtttgta
     5641 ttgggatgct tataggtaat ttttccgctc tgattttcgt tgtaatagtt tccaacaaat
     5701 aaaagcgatt cagaggtatc attaaacttc tgacgtacgg taataaaatt gttagtgctc
     5761 gatgcgtttg ccaattgggc ttttgaacgc tctagtatgg ttgtgtaggc ttgcaaatgg
     5821 ccttcggtat caaatcctag ccaggtaccc aggtgaacta ctttgccctt tccaacattc
     5881 ttttcaaatc cgcaaatact tcccctcagg gtaaaggcta taggagtagc accatctata
     5941 gcctctgttg tataggcaat cagtgggttt gagcatttaa tatcggtcaa attataaagt
     6001 ttgattaagg gggaatcaat tatttccggt tgtgatgatt ttattttaaa agtatccctg
     6061 agtactgtgc aaggcttttg gttcatctcc ctatcgggta agtaggggaa aataaccaaa
     6121 ttaccaccat tagtcacaaa acttgcaatg gtttgctgat cgggagcatt catctcatcg
     6181 gtactaaaga gccaaacctg cttataggcc aacaaatcgt tcaccgatga gctggctagg
     6241 tcaaccatat cgtagtcaac attgagaacc tggagggctt tgagtaatcc atcgaaatat
     6301 gccgaccgac ggatccgagc tggaacaaat tttagttctg aagctccgac ttcaggacgc
     6361 tctagctcag tagcgtagta tggtgagtag aaaaggacac atacctcggc ttttctttta
     6421 gtggttagga tgagttcttc atgggtttca ataaacctac tcattttctg aacaacaggg
     6481 aatgcactcg atggtttagc ttggttatct aagggtgtaa accagtaaaa agtatcaccg
     6541 gaatatccct ttgcgggtgg gtttttaccc tgtgaaaaca tgtagtaatt ccatcctttt
     6601 aggccattgg caatacttgc cttgtaaaag agttccattt cacgcggatt ggtaaccaca
     6661 tggtattccc ttgatccact ctgaaactca gcggcaaaca gaggtttttc accctgcatt
     6721 gcactaacaa tgtcgttgat gattctatcg tcgtggaggt tcctatacga gacaaattca
     6781 ggaatgtggt caactccaaa tatcaactca cttttatcct tatataggtc ctcgtacatg
     6841 gtaatattga gggggaactc gtacccatga ccgtaaatcc atccgggaag gttatggtat
     6901 aagggaattg aaatattgtg cttacgaatc caactggata gtaatcgtaa gtagttagcg
     6961 taatactttc tccagaaaca gtgccattcg taatcgcggc tacgatcgcc tttggattta
     7021 taaggggtat tcccatcggg gggtaactca atttcctgaa acgaggtgta attagtttcc
     7081 caaagggtgt tcaactcatc aatcgttgaa taccttgttt tgatgaaact gataaattgc
     7141 tcttttacct gttcattgta atcggcttgg tgggcaagcc atgagaaaac tccaatttcg
     7201 ttgcaaacct gcaccattat tataggtccc ccattttgta tctgtttctg gcaaataacg
     7261 ggcattacat ggttatacca ttgttctact ttggaaagaa atgcggggct aaacaggcta
     7321 acgccatcgc tcagaacggc ctcgcctttg ctgttatgca ttttaatagt gtcgccatac
     7381 ctttccagaa accaatcggg caaaccagcg cctctgtatt cagcaagtat aaaaggacca
     7441 ggcttaacaa tacaggtaaa accatgtttt tggcaaagct ctatccatcc tataagatcg
     7501 cgttcaggtt gagttgttcc actaaagtca aactcacctt cgttaacctc gtgccaaacc
     7561 caaggaacat agctgctaat ggtttttagc cctgcctctt tggctttttt cagatgttct
     7621 tcccatagct cacgtttaat tctgaaataa tgaatttcac cggagttcag aaatactttt
     7681 ctaccattca aactgaaata tccgccttca attccaaact tgttcatagt atttctattt
     7741 tttactggtt ctgtagtttg tttcgggttg ttaatgtttg ctttatttct tgaagccgct
     7801 tttcggtaag gttatatcta aatatgaata tcaacgaaag tatactcata ataactggca
     7861 atccaatttc aactactctc atcataagta aagtgaacgg gctctgacgt tttagggaag
     7921 gaattttttg ggttatttcg tccagcttat ggatgttgac ggaatcgata tcataaatgt
     7981 tatactcttg gagcacgcaa tttatttgtg caatgtccct tagtaactct ttatagtggt
     8041 ttccgtcacc tttaccggca attattgatt caaaatggtt cattagatcg taaacattgt
     8101 tttgagcttt aattaaatta aagttcattc tttgtttatt taatttttgt gcaatgattg
     8161 ataggttgac agagtcggga ctcgatgtat ttagaacaga atcccaatat tttaggctgt
     8221 cgatagtaat gcttaggttc tgtcgtaaag ctactttggc ttgaattttt aggctgtcgg
     8281 tttgaggatt ttcgtgctga tttactcttt ccaaaaaagc atcgtattta attgcattac
     8341 caagtatatc agtgttaata tcttttattt tttttatgta atcggtgtag ttcaaaacgt
     8401 ttttatcggt tagatttact ttgatttcct ttatgctacc ttcaaggtag tcaacttttg
     8461 ttacctggtt ctggtcgaac tgagtgaact caataaggat tccggcaaca aagctggcca
     8521 cggcaaatcc cattttaata aaccaccaga aaaccgagta aaagcttcct tcctttctat
     8581 gcccagtatt taagtcatcc tcatcgcaaa tatcacctac cattgaggag ccaagggtga
     8641 agaagaatag cattcctgct gagagcagaa tagttggaat aattatcagg tacgggtatt
     8701 cagggttata acaaactatt tttgaaagct gtgcggtggc cattaatgca atggatagga
     8761 ttaatgtatt gcgtttgccg agtttagggc ttatccagtt caatggaaaa actgctaaaa
     8821 gccctgtaac agcccatatg gttccattaa tgccaagcag atatgctcca gctaccttat
     8881 cgcctgaaaa aacgtaaaaa ataggtatgt atgagcccaa cagcgttaca aagttgaaac
     8941 cggttgcaag ggtaaaaatg gcaagagtaa gcctaataaa attcctgttt tttgtggtat
     9001 gcttcatgtc ggtccagaaa ggttttttct cctgcttctg caccctcaca aagctaggtt
     9061 ctcgtagttt tgcagtccac caaaaggaga ggggtataag caaagctgca atcattaatg
     9121 aaacatagcg cattccatcg gcttcgttcc ctccggggcc tttaaatgtt tccaggttgg
     9181 cgagcacaaa gagccatggg gtgctcatgg caaaaagatt tcccacaaaa ctttttgcgc
     9241 taaacagccg tgtacgctca tgggggtcaa cggtcatttc catccctaat gcaccatgag
     9301 ggatttcaaa tattgcgcac gaggtgtaga aaagcagaag tgttacaagt atgtaggata
     9361 gctgaaacca ctgatagccc gactcagagg ggaaccaaat tcgtatggtt tcgcctttgg
     9421 gtatccacca catggccaca aagaacaatg ctaccagtat tcctcctacc agtaaaaagg
     9481 gtcgtcggcg gccccagcga gtgcgagtat tatccgatag atgcccaata atagggtcgg
     9541 ctattgcatc ccaaagtcgt ggtataatta gtattgttcc caaccaaaaa gcgcttaagc
     9601 ctaagcttat gttccccatg agcccaacaa ggatgccagc aatgttaaaa agggcaatgg
     9661 gtattatgcc gccagcccca taagctaata gctgtgatat ctttatccta tattcacttt
     9721 ttgcctgtaa attattgttt tccattacat taagttatta gtagtaaatt tttatttcgc
     9781 aggggatttc attaactcgt atctcattta cgttgtaggt attaagctta gtaccattca
     9841 cttctacccc tttgggaagc ttagaaccat taaagtttct gatacagatt ccattggctg
     9901 gaactctaat atttccgcca atttttattt tgtagtggtc gtttcctttt gtaatagagt
     9961 aagataagtt cccgtagtat gttggaaggt tttgaaccga aattccttga gggctgtcaa
    10021 tccaatcctg tttaagagct gagccaatac ataaggtgtt gtggtcaata tcttcgtaaa
    10081 caaaaatatt tctgaaggca ttaatgaagt cgctacctac ccaggtgtga ggcatatcgc
    10141 ctatgtaagc tggagttcta taatcattcc aaaccacttc agcccagtga taccatcctt
    10201 ggggccgttg ctggcttagg aagtaatcaa ttaactcatg ggctctttcg ggttgattga
    10261 gcattatgaa cgagccaata attctgttct catagggtgt aaagttgacc cagtttattt
    10321 ggcccgtttt tctctggcta aagtatgtat agtatctttc aaaggtattg taaatttcag
    10381 gtttaggaag attttcgaac tcattgcaag gagtaagtgc aattgttgtt gatgtggcat
    10441 caaaatctcc cagctcggca catcctggta tatagttaat attatggttc ttaatggtca
    10501 ggtttatcga tttatatagg tttttcctga aagtatcggt tacttgctta atgcgcgtat
    10561 atttttcatg atttcccaaa gcgatttgaa tatcgcaggc atccttgagc cctttaagga
    10621 tgaagaaatt atcccagtat gagtgcatag gttttgctga gtatccctcg tggctaattg
    10681 attcgggaac taaaccataa aaggctctca gactatcgtt tccatacctg aaataatcag
    10741 tggatcgttc ggctatcaac gattcgatgt aacctattgt tctgaggata taaggattca
    10801 tctttcgcag aaatgcggta tcgtgtgtaa aactgaagta ttcatgtatt aagtagataa
    10861 attgaccgtg actgtcatgc tctgcaacag ggtcgggacc tctaaagtct acaacacagg
    10921 gaaccttgcc gttttcataa agattttgag cataccactc aataaattcc ttaacttcat
    10981 cagtaatgcc cgatttcagt aatgccgatg aagtaagcga accatcacgc atccaacttc
    11041 tctcatatga acgggaaccg ggttgtatgc catgaatatc gcggttaatt aaaatataca
    11101 ttaggttggc acggtaggtt tttaataaat gttcggcagt gctgggtaaa ttgaatttta
    11161 cgtattcggt tttgccgcgc cagtagtccg acacttttgc aattgtatcg acgatatttt
    11221 ttgtttttaa ttctgagtca ttgatatttt ttgaatgata tggagcaata gcaaataaag
    11281 tatgggattg tccggccttt agctttatgg tgtatttaac aaatccacca tttaaaccca
    11341 aattgtcagt ttctgccatt ac
//
LOCUS       DAOOWJ010000084        11349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5710_length_11349_cov_7.061360, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000084 DAOOWJ010000000
VERSION     DAOOWJ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5710_length_11349_cov_7.061360"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /locus_tag="PLW22_08615"
     CDS             complement(<1..1997)
                     /locus_tag="PLW22_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOU74806.1"
                     /translation="MRKNLLGLVSICLMTCLLWVNQTQAVPAYPGLVQYKQANGKTLA
                     IFLKGDEKVKWATTEDGYTIMLNSNGVYEYAIQDMKGDLQLSGIEVNNIKERTAAEKE
                     LLSKVSKGLYYSPSQLSMMKSIWDIKSAEAQKAFPTTGNRKLICILMGFKDKAFTKTQ
                     SDFNNLFNQVGYSTGGATGSVKDYYLENSWGQFNLTVDVFGPYTASQNMSYYGANDAY
                     GNDKNPRALVTEAVNAADASANFANYDNDGDGTVDGVYVIYAGYGEEAGASADAIWAH
                     AWSITPVTKDGKTISKYSCSAELRGNSGTTITSIGVICHEFGHVLGAPDYYDTDYSTG
                     GQFEGTGKWDMMASGSWNNNGVTPAHHNAYTKVKVYGWATATVLSSATNVTVKPVKDN
                     KSFFQINSTTSGEYWIMENRQQVGFDAYVPGHGLIIYHVHKDVASASSSNNINATYPQ
                     KMYPVCASATTNPGTTSSTYGSINSTGCPFPGSSNKTSFTDATTPNMKSWAGANTGKP
                     MTSIAENATTKDITFAFMGGSGGGTAPTATTVAASSITTSSATLNGTVNANNATTTVT
                     FEWGTTTSLGNSINATPNSVTGTSNTNVTANLTGLSANTKYYYRVKAVSANGTTYGST
                     MNFTTAVNPTSVTLPYSQTFSSSSMPTGWTTQNTGSGITER"
     gene            2260..2961
                     /locus_tag="PLW22_08620"
     CDS             2260..2961
                     /locus_tag="PLW22_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU74807.1"
                     /translation="MDLKAIIVDDEPLAIAVIDGYLSRIPGVEVVATFNDGLPAFEYL
                     QENPVDIVFLDVEMPKLTGIELVRCLYNPPVVIITSANRDYAIEGFELNVADYILKPV
                     TFERLLKAFSKAKELLSKRAENTSPKDSEYLIFKESRKSVRVKQDEILYFESIKDYVK
                     VVTPNRTVITKQSISSLEELLDKEKFIRVHRSFIVALNRVDTFSTTSLEIGDIEIPIG
                     RLYKEDVIKILETKK"
     gene            2968..6219
                     /locus_tag="PLW22_08625"
     CDS             2968..6219
                     /locus_tag="PLW22_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU74808.1"
                     /translation="MLRRLKYPLLIIVLVQLTFANAQERGLLLSHYYSANTYQAATQN
                     WGIVQDKRGVIYFANGAGVLEFDGKNWQFIEVGKGASVRSIAIDSNDRIYVGGYGEFG
                     LLSPDKTGLLRYVSLSSHVDSAYKEFNEVWDTRVIADTVFFLTDRCVFSYYKGKISYF
                     PLEGNRVFYLSHVLGSRYVAHILNEGMFFIDSGNLKLIKGSQQFSSLKIHSVIPFNGK
                     YLLCTRENGLYSVDYENGVFENFNSLSVLSPNAQSVDSYFKTNLFYHGISVNDSLLAL
                     SSILGDVIIVDQKFRVVDVIDQCSIGVRSTANFLYYQPSGILWLALNNGIASVELSSP
                     FRYWNEQVGIDGVLSDVARKGDFFYLSTGSGLFYIEKRRKETFKPDRFNRLEGRYEQA
                     WQFLYFQEPTDSALLKPLWYGSNHNTHLLVAARNGVFEINKRSANRISRLELPYVLHQ
                     GRINRSYLFVGHENGVSRLKYKNGKWHDEALLCKTNRKIVSIGEDSNGNIWFSENLGG
                     VGCILNAQYEKNAAEVIWYDTSKGLPDAAYVRIFDIYDTILFIANSKFYSFNQDAKRF
                     EPFDIKSLNILEWLPSNNDTLMWRRISNQLLTDQYVTHFADSISWVSTDKGIFQVRPA
                     KGYNYFNLPAPIIYRAINMDSVLFNGNNYSLVEGDTVFGAYLYKKIADPIIDLGIVLP
                     YKRNSVKFSYSWPYFESDQPCQFSYQLVGNDEGWSEWSTDYRKEYTNLREGKYVFKVK
                     ARSVFGVESPVLEFRFEVKTPWYRTIYAYIGYALLVFLLIYSIFKIWHYKLILERDKL
                     ERLVKERTQEILLQKEELQVQAEHLRETYEWISEKNAMLEKQKSEIEQQKQKLEEINA
                     TKNKFFRIIAHDLRNPISTLVNSTAFLLTDIETLSSEKTKKFMGELNRLALTTYGLLE
                     NLLDWSSNEMGDIQNKPQRVSLALLVNQNIELVQNRLTDKNIKLTVDIPASIELYVDE
                     NMLNTVLRNLISNAVKFTRPGGEISIDASEVNGKCVIRVKDNGIGIPDQNIEKLFRID
                     KSIVTPGTQNEKGSGLGLLLCKEFVDKMGGTISVNSRQGFGTTFSIEFPVDIL"
     gene            6300..7505
                     /locus_tag="PLW22_08630"
     CDS             6300..7505
                     /locus_tag="PLW22_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOU74809.1"
                     /translation="MKRIKYFFSLLLFLIMAIGVVFAQDKKDELKEPYAFTDVKVIPT
                     TSVKNQNRSSTCWSFSGMAFLESELLRMGKPAVDLSPMFVVRNIYAMKADRYVRFNGT
                     NNFGPGGSFFDVLEAIKRFGIVPMEVYQGLSYGEESHVHGEVDAVTKAFVDAIVKNPN
                     RKLSTAWKKAFDGILDAYFGSLPQNFTYNGKTYTPELFAKQLGINPDDYVVITSFSHH
                     PFYQKFVLELPDNWIHGEAYNVPLSDFDRIIESAIDNGYPVAWASDVSDKGFNWSKGV
                     AIVPDFDDKENAGSDKERWTQLSNREKEKTLYSFEKPVKEMVITQEIRQLAFDNYQTT
                     DDHGMVLVGKATDQLGNAFYKVKNSWGADGKYKGYFYASRAFVLYKSTNIMVHKKAVP
                     VDLAKKLGI"
     gene            7615..7986
                     /locus_tag="PLW22_08635"
     CDS             7615..7986
                     /locus_tag="PLW22_08635"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU74810.1"
                     /translation="MTNLYIKGTTKTPEIDFKPGILQISGRSIAEDAVAFYQPVIKWI
                     EDYLKKPEPLTRLNLKMEYINSGSNRFIFTIMRMLDEAYSQGHNVVINWYFEEDDDTI
                     KNLGRDFQALVKAPFKMVEII"
     gene            complement(7983..8681)
                     /locus_tag="PLW22_08640"
     CDS             complement(7983..8681)
                     /locus_tag="PLW22_08640"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOU74811.1"
                     /translation="MKMLWLTLQADSFQLFGIDIINTNDFYTLLLRFILNTVILWIAV
                     RYLYYPRTYRKDYVFTYLMLGVVVFFMCFLLANVKLQLGFALGLFAVFGIIRYRTNPI
                     PIKEMTYLFFIIAISLINAISSKKVSYAELLFSNFALLGVALILEKGLFIKHLSQKVI
                     VYEKINLITPDKRAELIEDLKKRTGLEIKSVEIGKVDFVRDIAWLVVHFPAENFNDTN
                     TPQIDAREVVDDDD"
     gene            complement(8650..9423)
                     /locus_tag="PLW22_08645"
     CDS             complement(8650..9423)
                     /locus_tag="PLW22_08645"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HOU74812.1"
                     /translation="MKENDIKQLDFNYLNLDDVGKAQFMRRIDSKYLVQAEWAFEILS
                     FLKQHYFIVENKAQLMPEYFSEYFDSSGFTMYLDHHNRRAKRYKVRLRHYRASGDVFL
                     EVKTRMPSGETLKKRTEITNNSIDNPTVEAFVKKNIPFRFNELTRTLYTTFNRITLVS
                     FDFNERVTMDFNIQLKNPTHAVNLSDICVIESKRDKYNSSSFLINHLKQHGYRQTGFS
                     KYSIGCALLYNNLKSNNFKPIITHINKYKNENALVNTTS"
     gene            complement(9437..9853)
                     /locus_tag="PLW22_08650"
     CDS             complement(9437..9853)
                     /locus_tag="PLW22_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74813.1"
                     /translation="MKTKYQLIVVALVLLIACPSFSFAQKKKEIRKAGIKQKVEWRYI
                     YEDDKEIKYKEYEARFDKQGNILMEKTYDEKGNTIKHIEYKYDNEGNEIVQTTYNSKG
                     QMLEKVETSYSNGLKVEKKVYGPNGKLKSRKVFEYK"
     gene            9897..10058
                     /locus_tag="PLW22_08655"
     CDS             9897..10058
                     /locus_tag="PLW22_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOU74814.1"
                     /translation="MIMYKCKVCGYIYDENIGDPDRGIPAGTPFEELPDNWHCPVCSV
                     TKDYFEEFK"
     gene            complement(10156..>11349)
                     /gene="pepD"
                     /locus_tag="PLW22_08660"
     CDS             complement(10156..>11349)
                     /gene="pepD"
                     /locus_tag="PLW22_08660"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HOU74815.1"
                     /translation="DFNNDPIQTYVDGEWLKAKGTTLGADDGIGIAAQLAILASNDIG
                     HGPLECLFTVDEETGLTGAFEMKSGFFNSKILLNLDSEDEGELFIGCAGGIDTVATFA
                     FEPDRVPGDSVAMKLTVKGLLGGHSGDDIDKHRANSIKLINRFLWKGTQLYDLRLANL
                     DGGNLRNAIPREATAVFTTHQDDKAKLILTFESIRNQIYNEWRDAEPNLEITIDDAPL
                     PQFVIDEPTHFDLLNSLYACPHGVIAMSRQLKGLVETSTNLASVKFIQDNQILVTTSQ
                     RSAVESAKNDISNMVESVFRLANANIQHSDGYPGWAPNTNSQILEITKASYSKLFKVE
                     PVVRAIHAGLECGLFLEKYPYLDMISFGPTIKGAHSPDERLHIPSTLKFWELLLDVLK
                     NIPEN"
BASE COUNT         3266 a         2119 c         2459 g         3505 t
ORIGIN      
        1 caacgttcag ttataccgct acctgtgttc tgagttgtcc atcctgttgg catcgatgag
       61 ctactaaagg tttgggagta cggaagagta actgaagtgg ggttaacagc ggtggtaaag
      121 ttcatggtgc tgccataggt ggttccgttg gcgctaacag ctttaaccct gtagtagtat
      181 ttagtatttg ctgataaacc ggtaaggtta gcggtaacgt tggtatttga agttccggta
      241 acactattag gcgtagcatt gatgctgtta cctaaacttg ttgtagtacc ccattcaaaa
      301 gtaacagtgg ttgtagcatt attggcatta acagtaccat tcaaagtagc gctgctggtg
      361 gttattgatg aagcggctac agtggttgcg gttggtgcag tgcctccacc tgatccaccc
      421 ataaatgcaa aggtgatatc cttggtagtt gcattctcag caatactggt cataggcttg
      481 cccgtattag cgccagccca cgatttcatg ttaggggtag tagcatcggt aaatgatgtt
      541 ttgttgctac ttccggggaa tgggcaacct gtgctattaa tgctaccata ggtacttgat
      601 gtagtgccag ggtttgttgt tgcgcttgcg caaaccggat acattttttg tgggtaagta
      661 gcgttaatgt tattgctgct gcttgcactg gcaacatcct tatgaacatg gtaaattatc
      721 aagccatggc caggaacgta tgcatcaaaa cctacttgct ggcggttttc cataatccag
      781 tactcaccgc tggtagtaga gttaatctgg aagaaactct tgttgtcttt aactggttta
      841 actgttacgt ttgttgccga tgaaagaacg gttgcagttg cccatccgta aaccttaact
      901 ttagtgtatg cgttgtggtg agcgggtgtt acgccattgt tgttccatga gccgctggcc
      961 atcatatccc acttgcctgt tccctcaaac tgaccaccgg tgctataatc ggtatcatag
     1021 tagtcaggag cgccaagtac atgaccaaac tcatggcata taacaccaat agatgtaata
     1081 gttgttccgg agtttcctct tagttctgca gagcatgagt attttgaaat agttttcccg
     1141 tctttggtaa caggagtaat actccatgcg tgcgcccaaa tagcatcggc actggcacct
     1201 gcctcttcac cataacctgc ataaataaca tacactccat caactgtacc atcgccatca
     1261 ttatcgtagt tggcaaagtt agctgaggcg tcggctgcat taacggcttc agtaaccaaa
     1321 gctcttgggt tcttatcgtt accgtatgca tcgttagctc cgtagtacga catgttctgg
     1381 ctggcagtat aggggccaaa tacatcaacc gttaggttga attggcccca tgagttctca
     1441 aggtagtaat ccttaacgct accggttgca ccgccagtgc tatatcctac ctggttgaat
     1501 aggttgttaa aatcactttg ggttttagta aaggctttat ccttaaaccc catcagaata
     1561 catattagct ttctattgcc ggttgtgggg aaagctttct gtgcctcagc tgatttaatg
     1621 tcccaaatgc tcttcatcat tgatagctgg cttgggctat agtataaacc cttacttacc
     1681 tttgagagca gttccttttc agcagcagtt ctttccttga tgttgtttac ctcaatgcct
     1741 gaaagctgca aatcgccctt catgtcctga atggcatact cataaactcc attgctgttt
     1801 agcataatgg tgtaaccatc ctcagtggtt gcccacttaa ccttttcatc accctttagg
     1861 aaaatggcca gagttttacc attagcctgt ttgtactgta caagtcccgg ataagccggt
     1921 actgcctggg tttgatttac ccatagcagg catgtcataa gacaaatgct tactaaacca
     1981 agtagatttt ttctcatagc gtttttttgt ttaggttggt gtttagttgg atgcgcgcaa
     2041 ggtatataca ataggtgtta atgtcaatat ttttgtactc ttgaatgtgc ttatgtcgac
     2101 ataaaacagt ttttaacata cgaaatgcaa tttcaaaagc atataatgtt aaattattgt
     2161 atattaatat attgtataat ttttaatatt ttggatgcta agtatggaag tgcattatgc
     2221 taaaaagtat taaattgcga ggctaaactg taggttacaa tggatttaaa ggccattatt
     2281 gtcgacgatg aacccttagc cattgcggta attgatggtt acctctcccg tatacccggg
     2341 gtagaggtag tggctacctt taacgatgga cttcctgctt ttgagtatct tcaggaaaac
     2401 cctgtggata tagttttttt ggatgtagaa atgccaaagc ttacgggtat tgaacttgta
     2461 cggtgcttgt ataacccacc tgtggttatt ataacctcgg caaacaggga ctacgcaatt
     2521 gagggctttg agctaaatgt tgccgattat atccttaaac cagtcacttt tgaaagacta
     2581 cttaaagcat tctctaaggc taaagagctt ctttcaaaaa gagccgaaaa tacaagccct
     2641 aaggactcgg aataccttat atttaaggag agtagaaaga gcgtaagggt aaagcaggat
     2701 gaaatactct actttgaaag cataaaggac tacgttaaag tagtaacacc taacagaaca
     2761 gttataacca aacagagtat aagtagctta gaggagttgc tcgataaaga gaagtttatt
     2821 cgtgtacacc gctcttttat agttgcactt aatcgtgtgg ataccttttc caccacctcg
     2881 ctagaaattg gagatattga aatacctatt ggtaggctgt ataaggagga tgtgattaaa
     2941 atactggaaa ctaaaaaata aaattttgtg ttacggagat tgaaatatcc gttactgatc
     3001 attgttctag ttcagcttac ctttgccaat gcacaggagc gtggattgtt gctttcccat
     3061 tactattcag caaataccta tcaggctgct acgcagaact ggggtatagt tcaggataag
     3121 aggggtgtta tatactttgc aaatggagca ggggtgcttg aattcgatgg aaagaattgg
     3181 caatttattg aggttgggaa aggtgcctcg gtcaggagta ttgcaattga tagtaacgat
     3241 cgaatttacg ttggaggtta cggtgaattt ggtttgttaa gtccggataa aaccggcttg
     3301 ttgagatatg tgtcgctttc atcgcatgtc gattcagcat ataaggaatt caatgaggtt
     3361 tgggatacca gggttattgc cgatacggtg ttcttcttaa ctgacaggtg tgtctttagc
     3421 tattacaaag gaaaaatctc gtatttcccc cttgagggga atagggtgtt ttacctttca
     3481 catgttttag gttctagata cgttgctcat atcctcaatg agggaatgtt ttttattgac
     3541 agtggcaatt taaaacttat aaaagggagc cagcaatttt catctctgaa gattcattca
     3601 gtaattccat tcaatgggaa atatctgctt tgtactaggg aaaatggact gtattctgtt
     3661 gattatgaaa atggggtatt tgaaaatttt aactcccttt cagtactttc gcccaatgcg
     3721 caaagtgttg atagctactt caaaacgaat ttattttacc atggtatttc ggttaatgat
     3781 tctctacttg cactctcgtc aattttaggc gatgtaatca ttgttgatca gaaattcaga
     3841 gtggttgatg ttattgacca atgttcaatt ggtgttcgga gcacagcaaa tttcctgtat
     3901 tatcagccct caggtattct ttggctcgcc ctcaataacg gtatagccag cgttgaactt
     3961 tcttctcctt ttcgttactg gaacgaacag gttggtattg atggagtgct atccgatgtt
     4021 gcccgcaaag gcgatttttt ttacttgtca acaggcagcg ggttattcta cattgaaaaa
     4081 aggcgcaaag aaacctttaa gcccgatagg ttcaatcgtt tggaaggcag atacgaacag
     4141 gcatggcagt tcctatactt tcaggaacca accgattcag cattacttaa accactttgg
     4201 tacggaagca atcataatac gcacttgctg gttgctgcaa ggaatggagt ttttgagatt
     4261 aataaaagga gtgccaatag aataagccgg cttgagttac cctatgtcct tcatcagggt
     4321 aggatcaatc gttcttatct ttttgttggg catgaaaatg gtgtttcacg tttaaagtat
     4381 aagaatggaa agtggcacga tgaggcgttg ctatgcaaaa ctaaccgaaa aatagtaagc
     4441 ataggtgagg atagcaatgg aaatatttgg ttttctgaaa atttaggtgg tgttgggtgt
     4501 attttaaatg cacagtatga gaagaacgca gctgaagtaa tatggtacga cacctcaaaa
     4561 ggattacctg atgctgctta tgttcgaatt tttgatattt acgataccat actctttata
     4621 gctaacagca agttttactc ttttaaccaa gatgcgaaaa ggtttgagcc ttttgacata
     4681 aaatcgttaa acattctgga atggttgcca agcaacaacg acaccctaat gtggcgaagg
     4741 ataagcaacc agcttttaac tgaccaatat gttacccatt ttgcagactc aatatcgtgg
     4801 gtgtccaccg acaagggaat ctttcaggtt cggcctgcta aggggtataa ctactttaac
     4861 ctacctgccc caattattta tagggcgata aatatggact ctgtgctctt taatggtaac
     4921 aactatagcc tggttgaagg tgatacagtt tttggagcgt atttgtacaa aaaaatagca
     4981 gatcctatca ttgatttagg aatagtactt ccgtacaaaa ggaattctgt gaaattttca
     5041 tactcatggc catactttga atccgatcag ccttgtcaat tcagttacca gcttgttggt
     5101 aacgatgagg gatggtcaga gtggtcgact gattaccgga aggagtatac taatttacgt
     5161 gagggtaagt acgtatttaa ggttaaagca cgaagtgttt tcggggttga aagtcctgtt
     5221 ttagaatttc gtttcgaggt taaaacgcca tggtacagaa ctatttacgc atacattggc
     5281 tacgctttgc ttgttttttt actaatatat tcgatattta aaatttggca ttacaagtta
     5341 atactggagc gcgacaagct ggagcgcttg gttaaggaac gcacacagga aattctttta
     5401 caaaaggagg aacttcaggt tcaggcagaa catctcaggg aaacctacga atggatatcg
     5461 gaaaagaatg ccatgctcga aaagcaaaag agtgagattg aacaacaaaa acaaaagctc
     5521 gaagaaatta atgctacaaa aaacaaattc ttcaggatta ttgcacacga tcttcgcaac
     5581 cctattagca ctttggtaaa ctctactgcc tttcttttaa ccgatattga aacgttaagt
     5641 tcggaaaaaa caaaaaagtt tatgggggaa cttaataggc tggcattaac cacctatggg
     5701 ttacttgaaa acttactcga ctggtcatcg aacgagatgg gcgacataca aaataaaccc
     5761 caaagggttt cccttgcgct tttggtaaac caaaatattg agttagtaca gaatcgacta
     5821 acagacaaga atatcaagtt aaccgttgat atcccggcta gtattgaact ctatgttgat
     5881 gagaatatgc tgaatacagt tctgaggaac ttaatcagta atgctgtaaa atttacaaga
     5941 cctggaggtg aaatttcgat tgatgcttca gaagttaatg gtaagtgcgt aatcagagtt
     6001 aaagataacg gaattggtat tcccgaccaa aatatcgaaa aactgttcag gattgataaa
     6061 agcattgtaa cacctggtac ccaaaacgaa aagggttcgg ggttaggatt actactttgc
     6121 aaagaatttg ttgataaaat ggggggaaca atatcggtaa atagcaggca gggctttgga
     6181 actacattct ccattgagtt ccccgtagat atactttagc tcatctattc aaataatttt
     6241 agccggttaa accttatacg taaggcaaaa tcaaacttaa caaaccaaaa ttcatagtta
     6301 tgaagcggat aaagtacttt ttcagccttt tgctattcct aatcatggcg attggggtag
     6361 tgtttgctca ggataaaaag gacgagctaa aagagcccta tgcctttacc gatgttaagg
     6421 ttatacccac aacatcggtt aagaaccaga accgctcaag cacctgctgg agtttttcgg
     6481 gaatggcatt tctggagtca gagcttttac gaatgggaaa gccggctgtt gacctttcgc
     6541 ctatgtttgt ggtcaggaat atctacgcca tgaaagccga taggtatgtc aggttcaatg
     6601 gtacaaataa ttttggaccc ggtggttcat tcttcgatgt gctggaagcc attaagcggt
     6661 ttgggatagt acccatggaa gtgtatcagg gcttaagcta tggggaggaa tcgcatgtac
     6721 acggcgaggt tgatgccgtt actaaggctt ttgtagatgc aatagttaaa aatccaaacc
     6781 gtaagctctc caccgcttgg aaaaaagcat tcgatggtat actggatgcc tactttggtt
     6841 cattacccca gaattttacc tacaatggca aaacatacac accagaattg tttgcaaaac
     6901 agctaggaat aaatccagac gattacgtgg taataacctc gttcagccat catccctttt
     6961 accagaaatt tgttctggag ttacccgata actggataca tggcgaggct tacaatgtgc
     7021 ctttaagtga ctttgatagg attattgaaa gcgccatcga taacggctac ccggttgcct
     7081 gggcaagcga tgttagcgat aaagggttta actggagtaa gggtgtagcc attgtacctg
     7141 attttgatga caaggaaaat gccggttccg acaaggagcg ctggactcaa ctctcgaatc
     7201 gtgagaagga aaaaacttta tactcgttcg agaagcccgt taaggaaatg gtaattaccc
     7261 aggagataag acaacttgcc tttgataact atcaaaccac cgatgaccat ggtatggtgc
     7321 tggtaggcaa ggcaaccgat cagctgggga atgcatttta caaagtgaag aatagttggg
     7381 gtgccgatgg taaatacaaa ggatattttt atgcctcaag ggcatttgta ctatacaaat
     7441 caactaatat tatggttcat aaaaaagctg ttcctgttga tttagcaaag aaattgggaa
     7501 tttagtaaat acaccgaaca aatttatccg cccatcgttt aactattttg ggcggatttt
     7561 tttatttgta tggtataata tttataaatt tacatgctga taaagactta ggccatgaca
     7621 aatttataca tcaagggcac taccaaaaca ccggagattg attttaaacc gggtattctg
     7681 caaatttcag gacgctcaat tgctgaggat gcagttgctt tttaccagcc tgttattaaa
     7741 tggattgaag attaccttaa aaagcctgaa ccattaacca ggctcaacct gaagatggag
     7801 tacatcaata gcggttcaaa ccggtttatt ttcaccataa tgaggatgct tgatgaggcc
     7861 tactcacagg gacataatgt ggttattaac tggtactttg aggaggatga cgacaccatt
     7921 aaaaacttag gtcgtgattt tcaggccctg gtaaaggctc cattcaaaat ggtagagatt
     7981 atttaatcgt catcatctac tacttccctg gcatctattt gtggggtgtt agtatcgtta
     8041 aagttttcag cgggaaagtg aacaactagc caggctatat cgcgaacaaa atcaaccttg
     8101 ccaatttcta cactcttaat ttcaaggcca gttcgtttct ttagatcctc tataagttct
     8161 gcccttttat cgggggtaat gaggttgatt ttctcgtaaa ctatcacttt ctgtgataga
     8221 tgttttatga aaaggccttt ttcaagtatt agagccacgc ctaatagcgc aaaatttgaa
     8281 aataacagtt ctgcatagct aacctttttc gacgatatgg catttattaa cgatatggca
     8341 attatgaaaa agagataagt catctcttta attggtattg ggttggtccg gtagcgaata
     8401 atcccaaaaa cagcaaataa ccccaaagca aaaccaagct gtaacttgac gtttgcaagc
     8461 agaaagcaca taaaaaatac aaccacgcca agcataaggt atgtaaaaac ataatcttta
     8521 cggtaggtac gtgggtagta taggtaacgg acagcaatcc ataaaataac agtgttcagg
     8581 ataaaccgta ggagaagtgt gtaaaaatcg ttcgtgttta tgatatcgat tccaaatagc
     8641 tggaacgaat cagcttgtag tgttaaccaa agcattttca tttttgtact tgttaatgtg
     8701 cgtaattatt ggcttaaagt tattggactt taggttatta tacaaaaggg cgcacccaat
     8761 tgagtacttg ctaaaaccgg tttgtctata tccgtgttgc tttaaatgat ttataagaaa
     8821 gcttgacgag ttgtacttgt cccgttttga ttctattaca caaatatctg aaaggtttac
     8881 ggcatgggtt ggatttttga gctgtatatt gaagtccatt gtaacacgtt cattaaaatc
     8941 gaacgatact agggttatcc tgttaaaggt tgtatacaat gttcttgtta actcattaaa
     9001 cctgaaagga atattttttt taacaaaggc ttcaaccgtt ggattatcaa tggaattgtt
     9061 ggtaatttcg gttcgtttct ttaatgtttc gcctgaaggc attcgtgttt ttacctctag
     9121 gaatacatca ccactagctc tgtaatgcct gagccttacc ttataacgct tagccctcct
     9181 gttatggtgg tcaagataca tggtaaagcc actgctgtca aaatattccg aaaagtactc
     9241 aggcattagc tgtgctttat tctcaacaat aaagtagtgt tgttttaaga atgaaagtat
     9301 ttcaaatgcc cattctgcct gtactaaata cttactatcg atacggcgca taaattgcgc
     9361 cttgcccaca tcgtctaggt ttaagtagtt gaaatctaat tgtttaatat cattctcttt
     9421 catggattac actatactac ttatattcaa aaactttcct acttttcagt tttccattag
     9481 gcccatatac tttcttttct actttcaaac cattgctata gcttgtttcc actttttcaa
     9541 gcatttggcc tttactgtta taggtggttt gaactatttc gttcccttcg ttatcgtact
     9601 tgtattcaat gtgctttatg gtatttccct tttcgtcgta tgttttttcc attaaaatgt
     9661 tcccttgctt atcgaaccgt gcttcgtatt ccttatactt gatttcctta tcgtcctcat
     9721 aaatgtaacg ccattctact ttttgcttga tgcctgcttt acggatttcc tttttctttt
     9781 gagcaaaaga gaaggaagga caggctatca ataatactaa tgcaacaact atgagttggt
     9841 atttagtttt catttcagta aatttgataa attttttgca atttaatcaa aatagattga
     9901 ttatgtataa gtgtaaagtt tgtgggtata tatacgatga aaatattggt gaccccgatc
     9961 gtggtatacc ggccggaact ccttttgagg aactgccaga taattggcat tgtcccgtat
    10021 gtagtgtaac caaggattac tttgaggagt tcaagtagta ccctgtgtgc acgaaaacaa
    10081 gtagcccttt tattgaggaa aactaatgtg aaaaagaaaa gaacccgaat aaggttcttt
    10141 tctttttatt gaattttagt tttcagggat atttttaagt acatcgagta ataattccca
    10201 aaatttcagt gttgaaggta tgtgtaatct ttcatcgggc gaatgcgccc cttttatagt
    10261 tgggccaaac gatatcatgt cgaggtatgg atatttttcc aggaatagcc cacactccaa
    10321 acccgcatgg atagctctga ccacaggttc taccttgaat agttttgaat acgaggcttt
    10381 ggtaatttcc agaatttgcg agtttgtatt gggagcccag ccaggataac cgtcggaatg
    10441 ttggatgttt gcgttagcaa gcctgaaaac gctttcaacc atgttcgaga tgtcgttctt
    10501 agccgattca acggcactgc gttggctagt tgtaacaaga atttggttat cctgaatgaa
    10561 tttaacggat gccaaattgg tggaggtctc aactaatcct ttcagctgac gactcatggc
    10621 tataacgcca tgaggacagg cgtaaagtga gttgagcagg tcgaaatgcg tcggctcatc
    10681 aattacaaat tggggtaggg gtgcatcgtc gatggtaatt tcaaggttgg gctcggcatc
    10741 gcgccactca ttgtatattt ggtttcgaat gctttcaaag gtaaggatta gtttagcttt
    10801 atcgtcctga tgggtggtga atacggctgt tgcttcgcga ggaatagcgt tgcgtaggtt
    10861 gccaccatcg aggttggcta aacgaaggtc atagagctgc gtgcctttcc ataggaaacg
    10921 gttaattaat ttaatggaat tggctcggtg tttatcgata tcatcgcctg aatggccgcc
    10981 aagtaaacct ttaaccgtta gcttcatggc aaccgaatcg ccgggtaccc tgtcgggttc
    11041 aaaggcaaaa gttgccacag tatcaatacc cccggcacat ccgatgaaaa gttcaccctc
    11101 gtcttcggaa tcgaggttta ggagaatctt tgagttgaaa aatccgcttt tcatctcaaa
    11161 agcaccggtc aaacccgttt cttcatcaac cgtaaaaagg cattcaagtg gcccatgacc
    11221 aatgtcgttc gaggccagaa tggcaagttg tgctgcaatt ccaatgccat cgtcggcccc
    11281 aagggtagtg cccttggctt taagccattc cccatcaaca taggtttgta tggggtcgtt
    11341 gttaaaatc
//
LOCUS       DAOOWJ010000085        11099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_5857_length_11099_cov_8.056501, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000085 DAOOWJ010000000
VERSION     DAOOWJ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_11099_cov_8.056501"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2177
                     /locus_tag="PLW22_08665"
     CDS             <1..2177
                     /locus_tag="PLW22_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU74816.1"
                     /translation="LMLMKKLSEDKQGFNSIYMMLDSGARGSKEQIRQLCGMRGLMAK
                     PQKSGATTNEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTRRLV
                     DVSQDVIITESDCGTLRGLVATAIKKNEEVVETLYERILGRTTVHDVFHPLTGELIIA
                     AGEEITEEIAQIIEESPIEQVEIRSVLTCESRKGVCAKCYGRNLATGRMVQKGEAVGV
                     IAAQSIGEPGTQLTLRTFHVGGTAGSIISDSKIVARFDGRAEIEELKTVQRQDESGNN
                     YHIVIGRLAEMRIVDSNTGITLSTNNVPYGSKLYINNGDTVKKGQLICEWDPWNAVII
                     SEVSGKVGFDALIKEVTYREESDEQTGYREKVIIESRDKSKNPAVKILSDNEEVIKLY
                     NLPVSAHLTVEDGATIQAGDILAKIPRAVGKAGDITGGLPRVVELFEARNPSNPAIVS
                     EIDGEVSFGKVKRGNREIKVTSKTGEVKNYLVPLSKQILVQENDYVKAGTPLSDGAIT
                     PTDILAIKGPTEVQEYIVNEIQEVYRMQGVKINDKHFEVIVRQMMRKVEILDPGDTRF
                     LEGQLVDKWEFIEENDNIFEKKVVVDAGDAAHLRPGMIITARQLRDENSKLKRKDMKL
                     VEARDAVPATSNQVLQGITRAALQTNSFMSAASFQETTKVLNEAAIYGKVDTLEGLKE
                     NVICGHLIPAGTGLREFDKLVVYSKDEHQLIEKSKSSTATVK"
     gene            2292..2609
                     /locus_tag="PLW22_08670"
     CDS             2292..2609
                     /locus_tag="PLW22_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOU74817.1"
                     /translation="MNDKVNPINIELSDEVAQGIYSNLAIITHSSSEFIIDFVRIMPG
                     MPKAQVKTRVILTPDHAKRLLLALQDNMAKFEANMGTIKLPEGSEGMPPFPMNFGGKG
                     GEA"
     gene            2624..4690
                     /locus_tag="PLW22_08675"
     CDS             2624..4690
                     /locus_tag="PLW22_08675"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOU74818.1"
                     /translation="MNRVFTITMAFALAFVSCNKSDSLVVLSPSGKVSITFLINGKGQ
                     PSYSLLFDQKTIVKPSTLGFEFKDMPPLSDNIKVDSFKTRSFSEKWEMPWGDQRWVDN
                     TYNELKVFLSERKSPYRNFAVTFRAFNDGVGFRYEFPEQPGMDSVIILAENTNFNLTD
                     DHTCWWQPGDWDIYEHLYRTTPLCKIDAVNSQSKNPLAQTYIPENAVNTPVTMKTSDG
                     VYLCFHEADVNNYAGATLRVDTVNLSLQTALVGNDSGVKVKAKVPFNSPWRTIIITDK
                     PGDIVTSKLVLNLNEPNRIGDVSWFKPMKYVGIWWEMHLGKSSWDMASGKHGATTANA
                     KRYIDFAARNGIKGLLVEGWNTGWEHWIGFEEREGIFDFVTPYPDYNLKEVVRYAKSK
                     GVSLIMHHETSAAPRTYERQLDSAYRLMQSLGIHSVKTGYVGKIIPKGEYHQGQWMVN
                     HYRKVIETAARYQIAVDAHEPFKGTGEWRTYPNVIARENFRGQEFNAWSVDGGNPPEH
                     LTILPFTAQLAGPMDYTPGIFNIKLKPYKPDNQVNSTLAYQLALYVVIYSPIQMVADL
                     PENYEGHPAFQFIRDVGVDWEQTVVLGAAIGDYIAIAREERYSGRWFIGAITDENPRD
                     LSLKLDFLKPNVKYKATLYLDGPDAHWDKNPTSYRIETREVDSNTVIDLKLAPGGGAA
                     ISLFPF"
     gene            complement(4727..6145)
                     /locus_tag="PLW22_08680"
     CDS             complement(4727..6145)
                     /locus_tag="PLW22_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOU74819.1"
                     /translation="MYSFILAVLGLLLGYFLYGRFVERVFGANSQSQTPAYTMQDGVD
                     YIPMPWWKAFLIQFLNIAGLGPIVGAVLGAAYGPVAFLWIVLGNILAGAVHDYFSGMI
                     SIRMGGLSIPEITGKYLGISVKQFMRVFTLILMILVGAAFISGPAALMASLTPDYLTK
                     NVWVFIILTYYILATLLPIDKIIGRFYPIFGLALIIMAVGITIAIFTGGYLYDIPEIW
                     NNMHNMKANADKFPLFPMLFVTISCGAISGFHSTQSPMMARCLKNEKEGRKVFYGAMV
                     TEGIIALIWAAIGMSFYGGAQGLNSAIAGYNGEATIIVREISNTTLGAIGGILAILGV
                     VAAPITTGDTAFRSARLIVADFLKFSQKSLSNRVLVSLPLFIIGFTLTQVKFDIIWRY
                     MFWSNQVLATVVLWAITVYLAQEKKPYIITLLPALFMTAVVSNYITIAPEGFKLSHWV
                     GYVVGVLTPTVCLYVFAKYLKR"
     gene            complement(6252..8372)
                     /locus_tag="PLW22_08685"
     CDS             complement(6252..8372)
                     /locus_tag="PLW22_08685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOU74820.1"
                     /translation="MKSKGLLILTIGLMGTMMACTGKKGPENPFLGEYKTPFGVPPFD
                     KIKVEHYEPAIAEGIKQQLSEIDAITANAEAPTFENTIIPLDYSGELLERVVLTMENI
                     NSANTSDELQEVLKKVSPMLSKHRDDISMNPKLFERVKVVYENMAQNNYTPEQKLLIE
                     KTYKSFVRGGANLDSAKQAELRDINQKLSLLALQFNENLLAENNDYFIVIDKEEDLAG
                     LPESVKAMGAEEAKSRGMEGKWVYTIYKPSMIPFLTYAQNKELRDKLYYGYYMRGNNN
                     NAHDNKNIIKQIVDLRQKKAELLGYPTHAHYVLDVNMAKVPENVYNLLNKLWEPALKR
                     AKAERDDMQKLAKAEGAKYKIGPADWWYYAEKVRKAKYDLDEEMLRPYLSLDAVRSGI
                     FNLTEKLYGLKYVKRTDLPVYHPECEVYEVQEADGKHVGILYLDFFPRESKSVGAWCT
                     EYRSQRIMPDGKFISPVVSIVCNFSKPTGNSPALLSYDEAETFYHEFGHALHALFSKV
                     QYPGLRNVPRDFVELPSQIMEHWAGHKQYLKEYARHYQTGEPMPDELINKIEASGHFN
                     QGFATTEYLAASILDMDYHTLTADKAKIEDVVAFEKASMDKIGLIPEIWPRYRSTYFA
                     HIFSDVMGYSSGYYSYIWAEVLDSDAFEAFVESGDIFNKEIAGKFRTCVLEKGGSKDA
                     MELYKDFRGKEPSIEPLLKNRGLN"
     gene            8626..9972
                     /locus_tag="PLW22_08690"
     CDS             8626..9972
                     /locus_tag="PLW22_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOU74821.1"
                     /translation="MGAFHAYDIRGIYNKDFNKDDAYKVGFFLVDLLKTNRVLVGRDA
                     RESSDEIFEYLTRGITDAGADVYDLGLATTPMVYYATAKGNFNASVQITASHNPREYN
                     GMKVSRENAEPVGYDTGLKVIEEKIKNGEPIVPASSKGKVIEHNIRAEYIQFLNGFKR
                     DYSNLKIGIDCSNGMAALIIKDILGASPIYLYDELDGAFPNHEANPLIPENVVDLQNL
                     VKANGCDVGIIFDGDADRVMFVDENGRFISPDLMIGVLGHYFLEEKGLKGNVLQDIRT
                     SKAVGEYLSPMGATMHTWRVGRAFAAKKLREIDGIYGGELAGHYYFRDFFYSDSGMLA
                     CILILNVVSKMKAMGISLSQLISRIETYANSGEINFKLERKREAMDAVREHFTSVEQP
                     TASFDFDGYRVEFPDWWFNIRPSNTEPYLRFIAEAKTHKMLEEKVAKVREIIAKFS"
     gene            9982..10899
                     /locus_tag="PLW22_08695"
     CDS             9982..10899
                     /locus_tag="PLW22_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOU74822.1"
                     /translation="MNMNRLEIKGKHGTYLVNLADSTSVLKPSGEICSTYIGYEKQSG
                     RRVVVKRYHPWVNSASGYFWRIEREAQATSACSGLSSELILLEGIYYLVTDYIEGVSF
                     KELTRWRYHRKLNNYHLIYLASKALDALQKIHDTGFIHCDIKPSNIILTDPKNIANTD
                     VRIIDYGLARKPAEPLHSNEKALAFGLIYSAPEQVFNFWDLIDIQTDIYSMGVTLWAL
                     FSRSEPWVASNPLKTIHVQLTQPLPGNRRVADDLMSILLKSTSKAQLPKPPHYYTRTQ
                     LRALLQQAKSIRYASAVEMLKHIEQLGKR"
     gene            10974..11050
                     /locus_tag="PLW22_08700"
     tRNA            10974..11050
                     /locus_tag="PLW22_08700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11008..11010,aa:Pro,seq:ggg)
BASE COUNT         3093 a         2364 c         2496 g         3146 t
ORIGIN      
        1 ccttgatgct aatgaaaaag ctctccgagg acaagcaagg attcaactca atttacatga
       61 tgctcgactc cggtgctcgt ggttccaagg agcagattcg tcagctttgc ggtatgcgtg
      121 gtcttatggc taaaccgcaa aaatcaggtg ctactaccaa tgagattatt gaaaacccaa
      181 ttctttcgaa ctttaaggag ggtctttcgg tacttgagta cttcatctca acccacggtg
      241 cccgtaaggg tcttgccgat actgctctta aaacagccga cgctggttac cttacccgtc
      301 gtttggttga cgtatcgcag gatgtaatca ttaccgagag tgattgtggt acacttcgtg
      361 gacttgtggc tacagccatc aagaagaacg aggaagtggt tgaaaccctt tacgaaagaa
      421 ttcttggtag aaccacggta cacgacgtat tccatccgct aaccggcgaa cttattattg
      481 cagctgggga agagattact gaggaaattg ctcaaattat tgaggagtcg cccatcgagc
      541 aggttgaaat tcgttcagtg cttacctgtg aatcgcgcaa gggtgtttgc gccaagtgtt
      601 atggccgcaa cctggcaacc ggacgaatgg ttcagaaagg cgaggcagtt ggtgttattg
      661 cggctcagag tatcggtgag cctggtactc agcttacact gcgaaccttc cacgttgggg
      721 gtactgccgg tagcatcatt tctgactcaa agattgttgc ccgtttcgat ggtcgtgccg
      781 agattgaaga gctaaaaaca gttcaacgtc aggatgaaag tggcaacaac taccatatag
      841 ttattgggcg tttggctgag atgcgaattg tggattccaa cactggaatc accctttcca
      901 caaacaatgt gccttacggt tcaaagctat acatcaacaa tggtgatacg gtaaagaaag
      961 ggcaactcat ctgcgaatgg gatccatgga atgccgttat catttccgag gtttccggta
     1021 aggttggatt cgacgctctc atcaaggagg taacctaccg cgaggaaagc gacgagcaaa
     1081 ccggttaccg tgagaaagtg atcattgaat cgcgcgataa gagtaagaac cctgctgtaa
     1141 agatcctttc cgataacgag gaggtgatta agctttacaa cttacctgtt agcgctcacc
     1201 taaccgttga agatggtgca accattcagg caggtgatat tctggcaaag attccacgtg
     1261 ctgtaggtaa agcaggcgat attaccggtg gtctgcctcg agtagttgag ctttttgagg
     1321 ctcgtaaccc atcgaaccct gcaattgtat cggaaatcga cggtgaagta tcgttcggta
     1381 aggttaagcg tggtaatcgt gaaattaagg ttacctctaa gaccggtgag gttaagaact
     1441 accttgtgcc cctgtccaag caaatacttg ttcaggaaaa cgattacgta aaggctggta
     1501 cacctctttc ggacggtgca attaccccta ccgatattct tgcaatcaag ggtcctactg
     1561 aagtgcagga gtacattgtt aacgagattc aggaagttta ccgcatgcag ggtgtaaaga
     1621 tcaacgataa gcactttgag gttatcgttc gccagatgat gcgtaaggtt gaaatacttg
     1681 atccgggcga tacacgcttc cttgaaggac aacttgttga caagtgggag ttcattgagg
     1741 agaacgacaa tatcttcgaa aagaaggttg ttgttgatgc tggcgatgca gcccacctac
     1801 gtccaggtat gattattaca gcccgtcagc ttcgcgatga gaactcaaag ctgaaacgta
     1861 aggatatgaa gctggttgaa gctcgcgatg cagttcctgc aacatccaat caggtgcttc
     1921 agggtattac ccgcgctgcg ctgcaaacca acagctttat gtctgcagca tcgttccagg
     1981 aaaccacaaa ggtgcttaac gaagcagcca tttacggcaa ggttgacacc cttgagggat
     2041 tgaaagagaa cgttatttgc ggtcacctca ttccagcagg tactggtctc agagagttcg
     2101 ataagcttgt tgtttactca aaggatgaac atcagcttat tgagaaatca aagagttcaa
     2161 cagctactgt taagtaaatc agatagatta taggaaggag ccggtgaatg ccggctcttt
     2221 tttttgctca taatcgttaa ttagcaaaca aattcataac ttagccgtga aaacattaaa
     2281 accgtccagc tatgaacgat aaagtaaatc caatcaatat tgaacttagc gacgaggtag
     2341 ctcagggtat ctacagcaat cttgccatta ttacccattc atcctcggag tttatcattg
     2401 attttgtgcg tataatgccc ggaatgccca aggcacaagt aaaaacccgt gttattctaa
     2461 cgcccgacca tgctaaaaga ctcttgttag ctttacagga taacatggct aagtttgagg
     2521 ctaatatggg tacaattaag ttacccgaag gaagcgaggg aatgcctcca ttccccatga
     2581 attttggtgg taaaggtggt gaagcttaaa atactttttt gccatgaaca gagtttttac
     2641 cattacaatg gcttttgctc ttgcctttgt ttcgtgcaac aagagcgata gtttagttgt
     2701 tctatcacct tcaggcaagg tatctattac atttctgata aatggtaaag ggcaaccctc
     2761 atacagcttg ctttttgacc aaaaaacaat agttaaacct tcaacgctag gttttgagtt
     2821 taaggatatg ccacctttat cggataatat taaggttgac agtttcaaaa ccagatcatt
     2881 tagtgagaaa tgggaaatgc cctggggcga tcagcgatgg gttgataata cctacaatga
     2941 gcttaaggtt ttcctatcgg agcgaaaatc gccttaccgt aattttgctg ttacctttag
     3001 ggcttttaac gatggtgtag gatttcgcta cgagttccct gagcagcccg gaatggatag
     3061 cgtaattata ttagccgaaa atactaactt caatctaacc gatgaccaca cttgctggtg
     3121 gcagcccggc gactgggata tctacgagca tctataccgg acaaccccat tatgtaagat
     3181 tgatgctgta aatagccaga gtaagaaccc tttggctcaa acctatatcc ctgagaatgc
     3241 ggtaaacact cctgttacca tgaaaacctc cgatggggtt tacctctgct ttcacgaggc
     3301 cgatgtaaat aactacgccg gtgcaaccct aagggtcgat acggttaatc tatcgcttca
     3361 aacagcgctt gttggaaacg attcaggcgt gaaggttaaa gctaaagtcc cctttaactc
     3421 gccctggcga accataataa ttacagataa accaggcgat attgtaacat caaagctagt
     3481 gctaaacctt aacgagccca atagaattgg agatgtatcg tggtttaaac ccatgaagta
     3541 tgtgggtata tggtgggaaa tgcaccttgg caaatcgtcg tgggatatgg ccagcggaaa
     3601 acatggagcc acaacggcaa acgcaaagcg ctacatcgat tttgctgcgc gtaatggcat
     3661 aaaaggtttg ctggttgagg gttggaacac cggctgggag cattggattg gttttgagga
     3721 acgcgaggga attttcgatt ttgttactcc ctatccagac tataatttaa aagaggttgt
     3781 acgctatgcc aagagtaagg gtgtaagcct aatcatgcat cacgagacat cggctgcacc
     3841 ccgtacctat gaacggcaac tcgattctgc ttataggttg atgcaaagcc ttggcataca
     3901 ttcagtaaag actggatatg taggaaaaat tattcccaaa ggtgaatacc accaggggca
     3961 atggatggtt aaccactatc gcaaggtgat tgaaacggca gccagatacc aaattgctgt
     4021 tgatgcccat gagcccttta aaggtactgg tgagtggcga acctatccca atgttattgc
     4081 tcgcgagaac tttaggggac aggagtttaa tgcctggtca gttgatggtg gaaatccccc
     4141 ggaacacctt acaattctac cctttaccgc acagcttgct gggccaatgg attacactcc
     4201 gggcattttt aacatcaagc taaaacccta taagcccgat aatcaggtaa attcaacctt
     4261 agcctaccag cttgctcttt acgtggtgat atacagccct attcaaatgg ttgccgattt
     4321 gcctgaaaac tacgaaggtc accctgcctt ccagtttatc cgcgatgttg gggttgactg
     4381 ggagcaaacg gtggtattgg gtgctgccat aggcgattac attgccattg cccgcgagga
     4441 gcgttattca ggacgatggt ttattggagc aattaccgat gagaaccctc gagaccttag
     4501 cctaaaactt gattttctga aaccaaacgt taaatacaaa gcaactttgt accttgatgg
     4561 tcctgatgct cattgggata aaaatcctac atcgtataga atagagaccc gcgaggtgga
     4621 tagcaacacg gttattgacc ttaaacttgc acctggcggc ggcgctgcta tttcgctttt
     4681 cccattttaa taaatagtta aggctgccaa ttggcagcct tttttcctat cttttcaggt
     4741 atttagcaaa tacgtataaa caaactgttg gtgttaaaac gccaacaacg tagccaaccc
     4801 agtgtgaaag tttaaaaccc tcaggagcaa tagtaatgta gttgctaaca actgctgtca
     4861 taaataaagc tggcagcagt gttatgatgt agggtttctt ttcctgtgct aaataaacag
     4921 ttattgccca cagcacaact gtggcaagca cctggttact ccagaacatg tatcgccaaa
     4981 taatatcaaa ctttacctga gtaagcgtaa atccaataat aaaaagcggt aagctcacca
     5041 aaaccctatt cgataggctt ttctgcgaga actttaaaaa atcggcaaca attagtcgag
     5101 cgctcctaaa tgcggtgtcg ccagtagtta ttggtgcggc tacaactcca agaatggcaa
     5161 gtattccgcc aatggcgcca agtgtagtat tggaaatttc ccttacaata atagtggctt
     5221 caccattgta acctgcaata gctgagttta gcccctgcgc tcccccataa aaactcattc
     5281 ctattgcagc ccaaatcagt gcaataatac cttcggttac cattgctcca tagaatacct
     5341 ttcgtccctc cttttcgttt tttaggcaac gcgccatcat ggggctttgg gtggagtgga
     5401 agccgcttat tgctccacag gagattgtta caaacagcat tgggaataat gggaacttat
     5461 cggcattggc tttcatgttg tgcatgttgt tccatatttc aggaatatcg taaaggtagc
     5521 caccagtaaa tattgcaatg gtaattccaa cagccattat gatgagggct agaccaaaaa
     5581 tggggtaaaa acgaccaatt atcttatcga taggaagcag cgttgccaga atgtagtatg
     5641 tgagaataat aaacacccaa acatttttgg taagatagtc aggggttaac gatgccataa
     5701 gggctgctgg gcctgaaatg aatgctgcac ccacaagaat cattagtatt agcgtaaata
     5761 ctcgcatgaa ttgttttaca ctaatgccga ggtacttccc agtgatttca ggaatactaa
     5821 gcccgcccat gcgtatggaa atcattcccg agaaataatc atgaacggct ccggccagga
     5881 tattacctag tactatccat aaaaatgcta ctggtccgta ggctgctcca agtacggcac
     5941 ctacaatggg gccaagacca gcaatattaa ggaattgaat aaggaatgct ttccaccagg
     6001 gcatgggaat atagtcaacg ccatcctgca tggtgtaagc gggggtttgg ctttggctgt
     6061 ttgccccaaa tacccgctca acaaacctgc cgtataggaa ataacctaat agtaagccca
     6121 aaactgctag aatgaatgag tacatagatt tgattttttt ttagccaaat ttatttattt
     6181 cggttttatt ttttggggca gcaataaaaa aaagcagcta ttcacggctg ctttttctca
     6241 tttatcatgg tttagtttaa acccctattc ttcagcagtg gttcaatgct aggctcttta
     6301 ccacggaaat ccttatacag ctccatggca tcctttgaac cacccttttc aagaacgcaa
     6361 gtgcggaact tgccagcaat ttctttgttg aaaatatcgc cgctttcaac aaatgcttca
     6421 aaagcatcgc tatcgagtac ctcggcccaa atgtatgagt agtaaccgct tgagtaaccc
     6481 ataacatcgc taaatatatg tgcaaagtat gtgctgcggt aacggggcca aatctctggt
     6541 atcagcccga tcttgtccat agaggctttt tcaaaggcta ctacatcttc aatctttgcc
     6601 ttatcagccg ttagtgtgtg gtaatccatg tcgaggatcg atgctgctaa atactcggtt
     6661 gtggcaaaac cctggttaaa atgtccgcta gcttcaatct tattgattaa ctcatcgggc
     6721 atgggctctc cggtttggta gtggcgtgca tactctttca ggtattgttt atggccagcc
     6781 caatgttcca taatctggct aggtaactca acaaaatcgc ggggcacgtt gcgtaggcca
     6841 gggtactgca ccttactaaa aagcgcatgt aatgcatgcc caaactcatg gtagaaggtt
     6901 tcagcttcgt catagctgag gagtgctggt gagtttcctg ttggtttgct aaagttacat
     6961 actatcgata ctaccggtga aataaactta ccatcgggca ttatacgttg gctgcggtac
     7021 tcggtacacc aagctccaac cgatttgctt tcacggggga agaagtcgag gtatagtatg
     7081 ccaacatgct taccatcggc ttcctgaacc tcgtaaacct cgcattcagg gtgatatact
     7141 ggtaggtcgg tacgctttac atacttcaag ccgtaaagtt tttcagtgag gttgaaaatc
     7201 cctgaacgaa cagcatcgag cgataggtat gggcggagca tttcttcgtc caaatcatat
     7261 tttgccttac gcactttttc ggcatagtac caccagtcag cagggccaat tttatatttt
     7321 gcaccttcgg ctttggctag tttctgcata tcgtcacgct cagctttagc tcttttaagt
     7381 gctggttccc agagcttgtt caagaggttg taaacatttt ctggtacttt ggccatgtta
     7441 acatctaaaa cgtagtgagc atgggtagga taacccaaaa gttcagcttt tttctggcgt
     7501 aaatctacta tttgctttat tatattcttg ttatcgtgag cattgttgtt attaccacgc
     7561 atgtaatagc catagtacaa cttatccctt aactctttgt tctgtgcata ggttaggaaa
     7621 ggtatcatgc tgggtttgta aatggtataa acccatttgc cttccattcc cctcgatttg
     7681 gcttcctctg cacccatcgc ttttactgat tcgggtaatc cagccaaatc ctcctcctta
     7741 tcaatcacaa taaagtaatc attgttttct gctaaaaggt tctcattgaa ctgaagggca
     7801 agcagtgata gcttctggtt aatatcacgg agttcggctt gcttagctga atcaaggttt
     7861 gcaccacctc gaacaaacga tttgtaagtt ttttcaatca gcaacttctg ttctggggtg
     7921 taattgtttt gggccatgtt ctcgtaaacg actttaaccc tttcaaatag cttgggattc
     7981 atgctgatat catcccgatg tttcgaaagc atgggcgata cctttttaag aacctcctgt
     8041 agctcatcgc tggtattggc agagttgata ttttccatgg ttaaaaccac tcgctcaagc
     8101 agctctccgc tgtaatcaag cggtatgatg gtattttcaa aggtaggagc ttcagcgtta
     8161 gctgttattg catcaatttc gctcaactgc tgcttaatgc cctcagcaat agcaggctca
     8221 tagtgttcta ctttaatttt atcgaatggc ggaacaccaa agggggtttt gtactcgcca
     8281 aggaaaggat tctctggacc tttctttcct gtacatgcca tcattgttcc cattaaacca
     8341 atggttagaa ttagtaaacc tttacttttc atcgtctaaa agtttaagta ttgatagtta
     8401 gatttttagg tttgatttta atttcccgaa agttagcaat attgattata tttttatcaa
     8461 cccgggtaat gtttcgattt aatatcaccc ataaagttta ctttgtctct aattttttaa
     8521 tttttgggtt gatgatagtt gatactattt ttatgaatcg gtttagaata ttattttact
     8581 tttgcactct gtgaatagat gtttaacagg taaaaatgat gtattatggg agcatttcat
     8641 gcctacgaca ttagaggcat atacaacaag gatttcaata aagatgatgc ctataaggtt
     8701 ggattctttt tggttgacct gctaaaaacg aaccgtgtac tggttggtcg cgatgcacgc
     8761 gaatcgtccg atgaaatatt cgagtacctc acccgtggta tcaccgatgc cggagccgac
     8821 gtatacgatc ttggcttggc aacaacacct atggtttact atgccactgc aaagggtaat
     8881 tttaatgcat cggtgcagat tactgcctcg cacaatcccc gcgagtataa tggaatgaag
     8941 gtttcccggg agaatgctga acctgttggt tacgataccg gccttaaggt aattgaggaa
     9001 aagattaaaa acggtgagcc tattgttcct gcttcaagca aagggaaggt tattgagcat
     9061 aatattagag cagagtatat ccaattccta aacggcttta aacgcgatta ttccaacctc
     9121 aaaatcggga ttgattgctc caatggaatg gctgcactta tcattaagga tattcttggc
     9181 gcaagcccaa tttacctata cgatgagctc gatggcgctt tccctaatca tgaggccaac
     9241 cccctaatcc cagagaatgt ggttgatctg cagaatttgg ttaaggctaa tggttgcgat
     9301 gtgggtatca ttttcgacgg cgatgccgac cgcgttatgt ttgttgacga gaatggtcgc
     9361 tttatttcgc ccgacctcat gataggcgta ttaggtcact actttctgga ggagaaagga
     9421 ctaaagggta atgttttaca ggatatacgc acctccaagg ctgtgggcga gtatttatca
     9481 ccaatggggg caacaatgca tacctggagg gtaggacgtg cgtttgccgc taaaaagcta
     9541 cgcgagattg atggaattta tggtggcgag cttgcaggtc actactattt ccgcgatttc
     9601 ttctattccg attccggcat gctggcttgc atactcatac taaacgttgt gagcaagatg
     9661 aaagctatgg ggatatcgct atcgcagctc atatcacgaa tcgagaccta tgcaaattca
     9721 ggcgaaatca attttaagct tgagcgcaag cgcgaagcca tggatgccgt tcgcgagcat
     9781 tttacctctg tggagcaacc tactgcttcg ttcgattttg atggttaccg tgtggagttc
     9841 cccgattggt ggtttaacat tcgcccttca aacactgaac cctatctacg gtttatagct
     9901 gaagctaaaa cccacaaaat gcttgaggaa aaggttgcta aggtgcgtga aattattgct
     9961 aaattcagct aatacatata tatgaatatg aatcggctgg aaataaaagg taaacatgga
    10021 acttacctgg taaatctggc cgattctaca tctgttttaa agccaagcgg tgaaatttgc
    10081 tctacctata ttggctacga aaaacaatcg ggtaggagag ttgttgtaaa acggtatcac
    10141 ccttgggtta actccgcttc cggttacttt tggcgcatcg agcgcgaggc tcaagcaacc
    10201 tctgcatgct cgggcttatc ctccgagttg atactattgg agggcattta ctacttggtt
    10261 accgattaca ttgaaggtgt tagttttaag gaacttaccc gctggcgata ccatcgtaag
    10321 ctaaataact accatctcat atatcttgct tccaaagcat tagatgcatt gcaaaagatc
    10381 catgataccg gttttattca ttgcgacatt aaaccatcga atatcatttt aactgatcca
    10441 aaaaatattg caaacaccga tgttagaata atcgattatg gtttagcacg caaacctgct
    10501 gagccattac atagcaatga aaaagctctc gcttttggtt taatatattc cgctcctgag
    10561 caggtgttca atttttggga tttaatcgac attcaaaccg atatttactc aatgggagtt
    10621 acactttggg cgctattttc gcgtagcgag ccatgggtgg cgagtaaccc tcttaaaact
    10681 attcatgttc agttaaccca acccttacca gggaaccgaa gggttgctga tgatttaatg
    10741 agcatattgt taaaatcaac atcaaaggct caattgccca agccaccgca ctattacacc
    10801 cgaacccagt taagagcctt attgcaacaa gccaaaagta ttaggtatgc tagtgctgta
    10861 gagatgttaa agcatattga acaactggga aagaggtagc caaaatgtta aaaaagtttt
    10921 gctgctgtat tgtaatttaa aaaattcagt atctttgcac cgcaaaacaa tggcggggtg
    10981 tggcgcagtt ggctagcgca cttgcatggg gtgcaagtgg tcgtccgttc gagtcggatc
    11041 accccgacca aggtgaataa ggaggcagtc atgcctcctt ttttcatttc ctgcagttg
//
LOCUS       DAOOWJ010000086        10882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6018_length_10882_cov_7.419692, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000086 DAOOWJ010000000
VERSION     DAOOWJ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6018_length_10882_cov_7.419692"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..989
                     /locus_tag="PLW22_08705"
     CDS             264..989
                     /locus_tag="PLW22_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOU74823.1"
                     /translation="MCFTLSVRVEKHRLEKVMNARFREDFRFEPFYFVSAFDFPVIPV
                     VASGNPEYFTPMSWGLIPNWVKNPIEAQSIKEKTLNARFESILEKPSFRYSAKSNRCI
                     VPATGFFEWQHIHGKKIPWFIGLNNNEIMHLAGIYSTWTNTETGEMVDTFSIITIPAN
                     PLLEKIHNTKKRMPAILKPENAEKWLDKDLKTSLYSDIIKPVEQEYLKAYTVSPIISN
                     PKAKRNVPEVLEPYSYPEQQSLF"
     gene            1035..1736
                     /gene="sfsA"
                     /locus_tag="PLW22_08710"
     CDS             1035..1736
                     /gene="sfsA"
                     /locus_tag="PLW22_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOU74824.1"
                     /translation="MKFDQPLIHGRLVKRYKRFLADILLDNGNVVTVHCTNSGSMKSC
                     LEENAEVFISPSSNPNRKLPYTWEMIRINNGWVGINTSNPNRIAYDILKNHWIDELPT
                     FKTLIREVKYDDSRFDIYGESENQRWFFEVKNVTLKEGRFAQFPDAVTTRGQKHLSTL
                     IKAKAEGYHVVMIYIIQRTDVDIFAPAWSIDSAYAKELIKAFDAGVKLFAVQVNVTPE
                     SIEPLRLIPIDLIQK"
     gene            complement(2152..2841)
                     /locus_tag="PLW22_08715"
     CDS             complement(2152..2841)
                     /locus_tag="PLW22_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74825.1"
                     /translation="MYENILDAYQVDSTYMPILMLKGKVESDMKKYSQAEITFTRIAS
                     LGDSSFFTLKNLAIAKHSNGSYLEAENLFIKAYEMDSTDFLLNFVYAKTLQNVGERNL
                     ALDIISNTIKLLYPPDELVASFYELAGDIYNSGNKIDLAVENYQMALEKDKKNELKYL
                     NKIIWCKIYDKKFRDAEYLVHKYDSIAQEKYADDSIQLKNEQRKTGFYKDLLKKEFFF
                     MDDLKNGKFLV"
     gene            complement(2842..3444)
                     /locus_tag="PLW22_08720"
     CDS             complement(2842..3444)
                     /locus_tag="PLW22_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74826.1"
                     /translation="MKRITFCIGLSVMSLLGYGQIGNEILTSIALGKYSVALEQINEL
                     PPSPELIQFKAIAFGGLNMWDSAVVYYSKLTNIDTQLAKKGLARCYESVGNSSKAIDL
                     YKQILADDSLNASIITRYANLLRSSSLHNQALEQFQKLIELDSTCAKYWEMLGDQYKA
                     MNMTVESTTAYNKAYLLNSNNLFVASKYFAQLIKSNVPLQ"
     gene            3513..3968
                     /locus_tag="PLW22_08725"
     CDS             3513..3968
                     /locus_tag="PLW22_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74827.1"
                     /translation="MKKILIALTLFVAGTNLLLAQNINYDPELAKKLNADEYGMRLYT
                     FVLLKSGEKQNLSKSETDSIFRGHMNNIIRLENEGKLFVAGPFGANPYGYRGLFIFYE
                     TDINKVEEMVNTDPAVSSGILKPEIFNWYGSAALPIYLDYHNKIWNKMP"
     gene            4323..5525
                     /gene="trpB"
                     /locus_tag="PLW22_08730"
     CDS             4323..5525
                     /gene="trpB"
                     /locus_tag="PLW22_08730"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOU74828.1"
                     /translation="METIISNIQHEFVQPLSNMLKYNGRYGEYGGAYVPDVLAKPLER
                     LANEFDRLTKDSSFQKEFVQLLKHYVGRPSPLYRAKRLSEQVGSTIYLKREDLNHTGA
                     HKINNTIGQVLVAKRMGATEIIAETGAGQHGVATATAAALMGIPCKIFMGAKDAERQA
                     LNVRRMKLLGAEVITTTMGTATLKDAVDAALMYYIEHPESFYLLGSQVGPHPYPRMVG
                     YFQSVIGNEAREQILMQEGRLPNAIFACVGGGSNAIGLFSGFLNDVEVEIYGAEGGGE
                     AIPGNTAATLTFGKPTVFQGTLSYCLVDDNGNPCESHSVAAGLDYPGISPQHAYLKDT
                     SRAKYFAVTDTEAIKAFEMLSQLEGIIPAIESSHAVALAAKMLKNKNQLAIVNLSGRG
                     DKDVEREF"
     gene            5654..6463
                     /locus_tag="PLW22_08735"
     CDS             5654..6463
                     /locus_tag="PLW22_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOU74829.1"
                     /translation="MKKILLILLLPIGLFTNAQEITKVENAVGRQVIAGSISEDEARA
                     KAIAQAKVDALRKAGVTEHIQSYEMLYKSEVGNKFDEVFMSDMFSEIRGAVRKYNIVK
                     IDKGIDEFKNFYVEAIIDAEVIQYTTAKDPAFQVSIDGIKQGYQSGERMRYTVTPTQD
                     CYLNIFNLYENNASLIYPNQFEKSRLFKAGEKVTFPLSELIDGYTLEKSAKEPETNKL
                     VFVFTKDNIPFIKYKLNQEGDQLTTFEDISAWLFSISPDRRVNYFIQFVIY"
     gene            complement(6453..7145)
                     /locus_tag="PLW22_08740"
     CDS             complement(6453..7145)
                     /locus_tag="PLW22_08740"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOU74830.1"
                     /translation="MKCLMSWSGGKDSALALYRCINDHNHNIVGLVTTINSSTNRVSM
                     HGVALDVLKYQADAIGIPLHVITLPEKCSMDEYESIMGAFMSRMKQDGVEGVVFGDIF
                     LEDIRKYRERQLEGIGITPIFPLWGLASAEVASQFLDNHFKSIITCVDSDLLDESFVG
                     QFYTSAYIEKLPGGVDPCGENGEFHSFVTAGPIFQKPIEVKVISKIRKEYPNPQGGNP
                     KTFWFADIAASK"
     gene            complement(7154..8227)
                     /locus_tag="PLW22_08745"
     CDS             complement(7154..8227)
                     /locus_tag="PLW22_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74831.1"
                     /translation="MNRKFFNLTIFAFLAFAFFSCEKDDKKPGKFENGAIVLNEGSFM
                     QSNASVSWVSYQSDSVVNNVYENVNARPLGDVLQGGVKANGKIYLVLNNSGKVVIVNS
                     SDFIQTGEVSNLNNPRYAVVVDGKLFVSQWNGWGQKGAVMVYNANGSEKIDSIPVGTG
                     AEGIIYAAGKVWVANSGGYGVDSTVTVINTSTLQVEKTINVGYCPKEFTVDANGKVWV
                     LCSGTTIYDANWNVAGHKPSSLAAINTSTMSVEKSVNLFNDMHPSHIDISVDKQTIYF
                     GGGFGFNGIYNVQIVASSISQTPLVSGYFYGFNVNPINGDIYTLEAPNFTSAGSLKVY
                     NSDGSGPKKTASVGIGPNGVLFN"
     gene            complement(8208..10175)
                     /locus_tag="PLW22_08750"
     CDS             complement(8208..10175)
                     /locus_tag="PLW22_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU74832.1"
                     /translation="MYFRVGVILYAFAISGITAARAQMCLEDTIRVEEVTIYSTYRQL
                     HTIGTNTIKIDTLRLTNFEGKSLTELLQSTGVNIRSYGVGGLATIAVRGGGSSHTAVV
                     WNGINLQSPLNGGVNLSQFPVSLFSSVTVQHGGNGTVYGSGAVSGIVILENGGLLLQP
                     NGARAGFTYGSGNTRGANASVKIGNSKSALSLKYSGLIADNDFEFINTYKFGNPRERI
                     TNAQAAQHGILADAVLRFDNNALWNISGWFTHNDKNVQTLMSSSQISQANQLDNNFAV
                     SSNLTGNLPFFSLKLRNAFINGDNHFTDPASGISSLNTYKQLVNEIELKKTMLKHQEL
                     VVGLGYSVDIASSQSYTENALRNRISAYASLVSHLLGSRIKLVLSLRDELVDGSLIPL
                     VASSGLEYKATKSLMLRASAASSYRLPTLNDLYWSSTTYAVGNPNLEPEYGWNTDLGF
                     DYTIIQNALNLKFSGSYFISRLNRWIVWLPDAQDNNRWKPSNISTGKSEGFESFIILG
                     VKLMPISIRADLSYTLTNSKIYDSGSYNGKPMIYIPRHKVGASISIIYGQFNFGYNHS
                     FTSERYTDELNTLPLYNLGDIYMGYSCKAWGSAFTIGVGVNNVWNEQYQLVRNYAMPL
                     RNYFIRLNIDFQSKSTNIINLKTFKNEQKIF"
     regulatory      complement(10280..10468)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(10687..>10882)
                     /locus_tag="PLW22_08755"
     CDS             complement(10687..>10882)
                     /locus_tag="PLW22_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74833.1"
                     /translation="ALIFFGSFLYQDKKERKNGSLPCFPLAMQSFSIALCEKYCVHRL
                     TYGSIHRVTNISPLRGWKRC"
BASE COUNT         3366 a         2281 c         2098 g         3137 t
ORIGIN      
        1 tgtgtgtcgc ttaaatctat ttctaacctg tagacttatt tcaggactag acaagtctgt
       61 ttaaaggact aatactgttc tgttttgagg aatataaatt gcctttacga atctgattaa
      121 ggggagggtt attagtaaat acctcctctt attttggttt gtatgatttc ttacttaact
      181 agatatcaaa ggttttgaat gttaataaga ttttaagcag gttagtcttg taaacaaaga
      241 aatgttaaat catccaacac ctaatgtgtt tcacgttaag cgtacgagtt gaaaagcata
      301 gacttgagaa agtcatgaac gctcggttca gagaggattt tcgctttgaa cccttctact
      361 ttgtatcagc ttttgatttt cctgttattc ctgtggttgc atcgggtaat ccagaatatt
      421 ttacaccaat gagctggggc ctaataccca actgggtaaa aaaccctatc gaagctcaat
      481 ccattaaaga gaaaacgctg aatgcacgat tcgaaagcat tcttgaaaaa ccatcgttcc
      541 gatactcagc aaaaagcaac agatgcatag tacctgctac cggttttttt gaatggcagc
      601 atatacatgg taaaaaaata ccatggttta ttggcttaaa taataatgaa ataatgcatt
      661 tagctggaat ttacagcaca tggactaata ctgaaacagg tgaaatggtt gacaccttta
      721 gcattatcac tataccggca aacccattac tcgaaaaaat tcataatact aagaaacgaa
      781 tgccagccat acttaagccc gaaaatgcag aaaaatggtt ggataaggat ttaaaaacct
      841 ccctttactc tgatataatt aaacccgtag aacaggaata cttaaaggcg tatacagtat
      901 caccaattat ttcgaatcct aaggctaaaa ggaatgttcc cgaggttttg gaaccctact
      961 cataccctga gcaacaatcg cttttttgaa cattcaaggt gaaaacctgt ttaaacgcaa
     1021 catctgtaca taatatgaaa tttgaccaac ccctaatcca cggcaggctt gttaaacgct
     1081 ataaacgatt cttggccgat attctactcg acaatgggaa tgtagttaca gtgcattgca
     1141 ccaattcggg ctcaatgaaa agctgcctgg aagaaaacgc tgaggtattc ataagcccaa
     1201 gctctaatcc aaaccgaaag ttaccctata cctgggaaat gattaggatt aataatggct
     1261 gggtaggaat aaacacctcg aatcccaaca ggattgccta tgatattctg aaaaaccatt
     1321 ggattgatga gctacctaca tttaaaactt taataagaga agtcaaatac gacgatagcc
     1381 gtttcgacat ttacggtgaa tccgaaaatc aaagatggtt ctttgaggtg aaaaacgtaa
     1441 cgttgaagga agggaggttt gcccaattcc ccgatgctgt gacaacacgt gggcaaaaac
     1501 acctaagcac tctgattaaa gctaaagctg aaggttacca tgtagttatg atatacatta
     1561 ttcaacgcac cgatgtggat atatttgctc ccgcttggag tattgactct gcatatgcta
     1621 aagagctaat aaaggcgttt gatgcaggag taaaactatt tgccgttcag gtcaatgtta
     1681 cacctgaaag cattgagccg ttaaggctca tacccattga tttaattcaa aaatgatatt
     1741 gactattcag taacgcagct ttcacattta ccgaaccagc ggtaacggtt acgagctatt
     1801 atgtcataga aaaaatccct tataggagta ggtatcagca agaatattga cagtattggc
     1861 caaaacccac taaggtgcca acaaatcagt aatgctgcgg ttgattttgt gtaaactttt
     1921 ccctttagaa caaaaaccat tgattcttta tactgcttac taaccccata acgactgaca
     1981 atcagacctg catcatttga gccaaacggc atgaaactaa atagttgcct tttatcgtaa
     2041 cgctttataa agcgtacgct tttttggcat agattacacg atgagtcgta tagaattata
     2101 taagaaaggt gcaccatact ataggaacac cttttgaaag ggatttgttt attaaacaag
     2161 gaatttcccg ttttttaaat catccatgaa gaaaaactcc tttttaagta aatctttata
     2221 aaacccagtt tttctctgtt cgttttttag ctggatgctg tcatcagcat atttttcctg
     2281 ggcaatgcta tcgtatttat gtaccaggta ttcagcatct ctgaatttct tatcataaat
     2341 cttgcaccaa ataatcttat tcaggtactt aagctcattt tttttatctt tctcaagagc
     2401 catttgatag ttttcaactg ccaaatcaat tttgttgccg gagttataaa tatctcctgc
     2461 tagctcataa aatgatgcta ccagctcatc gggtgggtaa agaagcttaa tggtattact
     2521 tataatatcc agtgccagat tgcgctcacc aacattctgc aatgtttttg cgtaaacaaa
     2581 atttagaaga aagtcggtac tatccatctc ataagccttt ataaagagat tttctgcttc
     2641 cagatatgaa ccgttgctat gcttggcaat agcaagattt tttaaggtaa agaatgatga
     2701 gtcgccaagc gaagcaatcc ttgtgaaagt tatttcggcc tgactatact ttttcatatc
     2761 cgattcaacc ttccccttaa gcataagaat tggcatgtaa gttgagtcaa cctgataggc
     2821 atcaagaata ttctcgtaca cttactgtaa tggcacatta cttttaatca gttgagcgaa
     2881 gtattttgaa gcaacaaaaa gattattaga gtttaacaga tatgctttat tgtatgcggt
     2941 agtactttca acagtcatgt tcattgcctt gtactggtca cccaacattt cccagtattt
     3001 agcacatgtc gaatccaatt caattagctt ttggaactgt tccaaagcct ggttatgcaa
     3061 tgaactactc ctaagcaggt ttgcatacct ggttattatg ctagcattca aggaatcatc
     3121 tgctagtatt tgtttgtaaa ggtcaatggc tttggatgag ttaccaacac tctcgtaaca
     3181 cctggctaat cctttcttcg caagctgagt gtcgatattt gtaagttttg aatagtaaac
     3241 cactgcacta tcccacatgt ttaacccccc aaaagctatt gccttgaatt ggattaactc
     3301 aggtgatgga ggcaactcat taatttgttc aagagcaacg gaatacttac ctaatgcaat
     3361 acttgttaaa atttcattgc caatttgacc atagcctagt aatgacatta cgcttaatcc
     3421 tatgcaaaat gtaattcttt tcatactttt gttttatttg atatgacagt atattaattt
     3481 aacgctttgt gttgaacatt attatatgaa caatgaaaaa gatattgatt gcattaacac
     3541 tatttgttgc aggcacaaac cttttgcttg cacaaaacat taattacgat ccagagcttg
     3601 caaaaaagct aaatgccgat gaatacggta tgcggttata cacattcgtt ctactaaaat
     3661 cgggcgagaa gcaaaacctt tcaaaaagtg aaacagatag tatttttaga ggccatatga
     3721 acaacataat ccgtttagaa aacgaaggta aactctttgt tgctgggccg tttggagcaa
     3781 acccatatgg ttacagggga ttattcattt tttacgaaac tgacattaat aaagtggagg
     3841 aaatggttaa taccgatcca gcagtaagtt caggaattct aaaacccgaa atttttaact
     3901 ggtacggttc ggctgcattg cccatttacc ttgattatca caataaaata tggaacaaaa
     3961 tgccatagcc caatttggat tttaaaaaaa atctttttaa gattgcaaaa gaatagacaa
     4021 cgatgaaacg attaagcaat atttggtggt ggcacaaatt aatccgaaca cagggggatt
     4081 agttaggcca ttgctttgca atatacagcc gtaggttacc ccctacggct tttttaattt
     4141 aataagtaga tgatgatcca gttttaccga caaatacaac aaattgctgt tttcgctcca
     4201 tggtggtggc ggggcctccc cgcatagcca ttgttttact gtgagctacg tgccacaaag
     4261 gttcaaatca tctatcaagt acatttatca agtcattttt taaccctaaa aattttatca
     4321 tcatggaaac aataatttca aacatacagc atgaatttgt tcagccttta agcaacatgt
     4381 taaagtataa tggccgctat ggcgaatatg gcggagctta cgttcccgat gttttagcaa
     4441 agccactgga acgcttggcc aacgagtttg ataggcttac taaggattct agttttcaga
     4501 aagagtttgt acagctactc aagcactatg taggaagacc ctcgccccta tatagggcaa
     4561 aaaggctatc ggaacaagta ggatcgacca tttatcttaa acgcgaagat ttaaaccata
     4621 ccggcgctca taaaattaac aacaccattg gacaagtact ggttgcaaag cgcatgggtg
     4681 caacggaaat aattgctgaa accggtgccg gacaacacgg tgtggcaacc gcaacagccg
     4741 ctgcccttat gggaattcct tgcaagatat ttatgggagc aaaggatgcc gagcgacaag
     4801 cgctaaatgt gcgacgaatg aaacttttag gagccgaagt gataacaact acaatgggaa
     4861 cagcaactct aaaggatgct gttgacgcag ccttgatgta ctatatagaa catcccgaat
     4921 cgttttacct tttaggctca caggttgggc ctcaccccta ccccagaatg gtgggttact
     4981 ttcaaagcgt tattgggaac gaagctcgtg aacagatact tatgcaggag ggccgtttgc
     5041 ccaacgcaat ttttgcttgc gttggtggtg gctccaacgc tattggtctt ttctctggtt
     5101 ttttgaacga tgtagaggtt gaaatatatg gtgcagaagg cggtggcgaa gccattcccg
     5161 gtaatacagc cgctactctt acatttggta aaccaacagt attccaggga acgctatcct
     5221 actgcctggt tgacgacaac ggaaacccgt gcgagtcgca ctctgtagct gctgggttag
     5281 actatcctgg catcagcccg cagcacgctt accttaagga tactagtaga gccaagtatt
     5341 ttgcagttac cgataccgaa gccattaaag cttttgaaat gctctcgcag ctggagggaa
     5401 taatccctgc cattgaatcg agccatgcgg ttgcgttggc agccaaaatg ctaaaaaaca
     5461 agaaccagct tgccattgtc aatctttccg gacgtggcga taaggatgtg gaaagagagt
     5521 tttgaggtaa ggtttggaat aatcgttgct ggtgtgaagt gcccttaatg ggcacttcgc
     5581 ttttttaatg ggaaatgctt gccactatca atattttgca cgaaatttga agagaaaagt
     5641 gaaaaacatc gccatgaaaa aaatcttatt aattctcctg cttccaattg gtctatttac
     5701 caatgcccag gaaataacca aagttgagaa tgcagtgggc cgacaggtaa tagcaggaag
     5761 tattagcgaa gatgaagctc gtgccaaggc aattgcccaa gcaaaagttg acgctcttag
     5821 gaaagccgga gtaaccgagc atatccaatc gtatgaaatg ctttacaaga gtgaggttgg
     5881 aaacaaattc gacgaagttt ttatgagcga tatgttttcc gaaattcgtg gagcagtcag
     5941 aaaatataac atagtaaaaa tagacaaagg gattgatgag tttaagaatt tttacgttga
     6001 agctataatt gacgctgagg ttatacagta cacaacagct aaagaccctg cattccaggt
     6061 aagcatcgat ggaattaagc agggttacca aagcggcgaa cgtatgcgtt acacggttac
     6121 accaacccag gattgctacc tgaatatttt taacctttac gagaacaacg cttcgcttat
     6181 ttatcccaac cagtttgaga aaagtaggct atttaaagcg ggcgaaaagg tcacctttcc
     6241 tcttagcgag cttattgatg gttataccct tgaaaagagc gcaaaagagc ctgaaacgaa
     6301 caaactggtt tttgtgttca caaaagataa cataccattc attaagtata agttgaacca
     6361 ggaaggcgac cagctcacta cctttgagga tatttcggca tggttattca gcatatcgcc
     6421 cgatagacgg gttaactact ttatccaatt cgttatttac tagctgcaat atcggcaaac
     6481 caaaaagttt taggatttcc gccctgggga ttggggtatt cctttcttat tttgctaatt
     6541 acctttacct caatgggttt ttgaaagatt gggccagcag taacaaagct gtgaaactcg
     6601 ccattttcgc cgcaagggtc aactccacca ggaagttttt caatgtacgc tgaagtatag
     6661 aactgaccca caaaactctc gtcaagcagg tcgctatcaa cacaggtaat tattgattta
     6721 aaatggttat caaggaattg gcttgccact tcggccgacg caagacccca gagtggaaaa
     6781 atgggagtta taccaattcc ctccaactgt ctttcacggt atttcctaat atcctcaagg
     6841 aaaatatcac caaatactac cccttcaacc ccatcctgtt tcattctgct cataaatgct
     6901 cccataatac tttcgtattc atccatacta catttctcag ggagggtaat aacatgaagc
     6961 ggtattccaa ttgcatcagc ctgatatttc agcacgtcca atgcaacacc atgcattgat
     7021 actctatttg tagaagaatt tatggtggtt acaagaccaa caatgttatg gttatggtca
     7081 tttatacatc ggtataaagc cagagcggag tccttgcccc cgctccagct cattaaacat
     7141 ttcataaagg tatctagtta aaaagaacac catttggacc aatacccacg gaagcggttt
     7201 tctttggccc gctcccatcg ctattgtaaa cctttaaact acctgcactg gtaaaatttg
     7261 gcgcctcaag ggtataaata tcaccgttaa taggatttac attaaaccca taaaaataac
     7321 ctgaaaccaa tggggtttga ctaattgaac tagcaactat ttgcacattg tatatcccat
     7381 taaacccaaa acctccacca aagtaaatgg tttgcttatc cacactgata tcgatatgcg
     7441 atggatgcat atcattgaat aaattgacac tcttctccac tgacattgtt gatgtattta
     7501 tagccgcaag cgacgatggt ttatgaccag caacattcca gtttgcatcg taaattgtgg
     7561 tacctgagca aagtacccaa accttaccat ttgcatcaac ggtaaactct ttagggcaat
     7621 agccaacatt tatagttttt tcaacctgaa gggttgaagt atttattact gtaaccgttg
     7681 aatctacacc ataaccacca ctattagcaa cccatacctt accggctgca tatatgattc
     7741 cctcagcacc tgtacccact ggaattgaat cgattttttc agagccattg gcgttatata
     7801 ccattacagc acctttttgc ccccagccat tccattgcga aacaaataat ttgccatcga
     7861 ccaccacggc ataacgtggg ttattgagat tactaacttc gcctgtttga ataaaatcag
     7921 agctattaac aataacaacc ttgccggagt tattcagtac taagtagatt tttccattgg
     7981 cctttacccc accctgaagc acatcgccca aaggacgagc attaacattc tcatacacat
     8041 tattcaccac cgagtcggac tggtaactaa cccagctcac cgaagcattt gactgcatga
     8101 atgacccttc gttgagcaca atggctccgt tttcaaactt cccgggcttt ttgtcgtcct
     8161 tttcgcacga aaaaaatgca aatgccaaga aggcaaaaat ggttaggtta aaaaattttc
     8221 tgttcatttt taaaagtttt taagttgatt atattagttg atttggattg aaaatcgata
     8281 tttaaccgaa taaaataatt acgcaggggc atggcatagt tacgcacaag ctggtattgc
     8341 tcattccata cattattaac acctacccca atcgtgaacg cactccccca tgccttgcag
     8401 gagtacccca tataaatatc accaaggttg tataagggca acgtgtttag ctcatcggta
     8461 tatcgctccg atgtgaagga atgattatac ccaaaattaa actgcccgta aataattgag
     8521 attgatgctc caactttatg cctggggatg taaatcatgg gtttgccatt gtagctcccg
     8581 ctgtcgtata tctttgaatt ggttaaagtg taacttaaat ctgccctgat tgaaattggc
     8641 atcaacttga ctccaagaat tatgaaactt tcgaaccctt ccgatttgcc agtgctaata
     8701 ttactaggtt tccacctatt gttatcctgt gcgtcgggta accaaactat ccacctattt
     8761 aacctgctaa taaaatagga accagagaat ttgagattta aggcattctg aataattgtg
     8821 taatcaaagc ctaaatcggt attccatcca tattccggtt ccaaattggg gtttcccacc
     8881 gcataggtgg ttgatgacca ataaaggtcg ttaagcgttg gcaaacggta gcttgatgct
     8941 gctgaagccc ttagcatcaa actttttgtg gctttatact ccagtccact tgaagcaacc
     9001 aatggtataa gcgaaccatc aaccaactca tcgcgaagcg agaggacaag cttaattcta
     9061 ctacccagca aatggctcac aagtgaggcg taagctgaga tgcggttccg taaggcattt
     9121 tcagtgtagc tttgggatga agcaatatca acagaatacc ccaaacctac aaccaactcc
     9181 tgatgcttaa gcatcgtttt ttttagctca atctcgttta caagctgctt gtaggtgttc
     9241 aagctgctaa taccggaagc gggatcggta aaatgattat cgccatttat gaatgcgttc
     9301 ctgagtttga gagaaaaaaa tggcaaattc cctgtcaggt ttgacgatac agcaaaatta
     9361 ttatcgagct ggttcgcctg ggaaatctga gagctgctca tcaaagtttg gacattctta
     9421 tcgttatggg taaaccaacc cgaaatgttc caaagtgcat tattatcgaa acgtaataca
     9481 gcatcggcta atatcccatg ctgagctgcc tgtgcattgg taattcgctc acgtgggtta
     9541 ccaaacttgt aggtattaat gaattcaaaa tcgttatcgg caattaatcc gctatacttc
     9601 aaggataatg cactttttga attacctatc tttactgaag cattagcacc ccttgtgttt
     9661 ccgcttccat aggtaaagcc agcccttgct ccgtttggct gaagcagtag gcccccattt
     9721 tcaagaatca caatccccga tactgcacca ctgccataaa ctgttccatt gccaccatgc
     9781 tgaactgtaa ccgagctaaa taggctaacc gggaattgcg aaaggtttac tcctccattc
     9841 aaagggcttt gcaggtttat tccgttccaa accacagcgg tatgcgatga gcccccaccc
     9901 ctaacagcta ttgtggcaag tccaccaact ccatatgaac gaatatttac cccagttgat
     9961 tgaagtaact cagtaaggct tttgccttca aaattcgtca accttaaagt gtcaattttt
    10021 attgtgtttg tcccgattgt atggagctgg cggtaagttg agtaaatagt aacctcctcc
    10081 acacgtatgg tatcctcaag acacatttgt gctctggcag ctgtaatgcc cgaaatggca
    10141 aaagcgtata aaattacacc gactctaaaa tacattgcta caaatgatta acggttttta
    10201 atcattcgca gcgctcggac ttagtgaaaa tagaacagaa aaaaagtttg tgttctatcc
    10261 ttcgcttttc acccgaaagc tacgaaaaaa gcaaattggc aggtcttctg gcttgctcaa
    10321 ccttttacag ccttcccata atgcaattac attacagtgg caggggagta aaaggccttt
    10381 gaatgagctt acagctgcgg ggacagcccc ggatttacac cgggttccct tttaagcctc
    10441 actaggcacc aataacactg caatattact ccgtttatat gaactatgca acagctcagg
    10501 gttatgtttt tgaacagaat gttaacacgg cacgaggcat agcgatttag tatcacattt
    10561 caatgtcgtt tagttaacaa aaatacacat gcctgtcatc ctgcgcacag cgaaggatct
    10621 ctaatcgacc ccatgagatg tttcgcttac gctcaacatg acaatagttt cacgagcaac
    10681 gaacaactaa caacgcttcc agccccgtag gggcgaaata tttgtaaccc tatgaatact
    10741 cccatatgta aggcgatgca cgcagtactt ttcacaaaga gcaatactga aactttgcat
    10801 cgctaaggga aagcaaggca aagagccatt tttacgttct ttcttgtctt gatacaagaa
    10861 agaaccaaag aaaatcaagg ct
//
LOCUS       DAOOWJ010000087        10792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6092_length_10792_cov_8.838875, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000087 DAOOWJ010000000
VERSION     DAOOWJ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6092_length_10792_cov_8.838875"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(345..2726)
                     /locus_tag="PLW22_08760"
     CDS             complement(345..2726)
                     /locus_tag="PLW22_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU74834.1"
                     /translation="MKYRLLALITVTFLVSSCQKSLRIEHATTSPAVHDLLSVIQLAG
                     DSTLVYLTDYFPDPQAIDSVSIPSHFQYSISDSKAYLTLKADNQSLPWLSYIFIWAKG
                     DKYCLLVRKNRKLPVTFTFDPQGKKVKSVQLAGQINDWNPSSTNLTFDGKVYKTTKLL
                     NPGKYHYQVVIDGKWMLDPANPTFEDNGMGGYNSVLVVGNTNPEELPKLVPFSTDGNK
                     LLIKAIGNVDKFIVLWQNYLMDDKFITSKEEFYEITIPENARGYKRSHIRIWAYNQQG
                     ESNNLLIPLDGNRVVINPSQLSRSDKHAQIMYFLLVDRFKNGNPSNDFKVNDPEIHPK
                     ANYFGGDLAGVTQKVKDGYFTKLGINTIWLSPITQNPLGAWGLYPQPRTKFSGYHGYW
                     PVSSSKVDFRFGTESELNELIAEAHKRNMNVILDYVANHVHQEHPLYKQHPDWATSLY
                     LPDGSLNTERWDEYRLTTWFDTFLPTLDLERPEVYEPMTDSALFWVSHYDLDGFRHDA
                     TKHIHENFWRRLTQKIKLNLPNKPVYQIGETYGSHELIASYIGSGMLDAQFDFNIYDA
                     SVAAFARNDYPFTKLDGALHQTFSYFGWINLMGYISGNQDRGRFISYAGGALRFGEDA
                     KKAGWTREIGVGDSTAYAKLCMLNAFNMTIPGVPTIYYGDEFGMPGGNDPDNRRMMKF
                     EGLSTLEQQTLETVTKLVNIRRNNLALVYGSFQTILVNDNVYAYVRKYFDNTVVVIFN
                     KSNQEQVIDLQLPEYMSDSVLYPNFNSKYSIQNGKLEVTLKPNSFEIFTTRIN"
     gene            complement(2747..4597)
                     /locus_tag="PLW22_08765"
     CDS             complement(2747..4597)
                     /locus_tag="PLW22_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOU74835.1"
                     /translation="MFKNRISLLMSTMFMVMTSLAQKPVERIEPPCWWVGMNNPNLQL
                     LVYGKNIASAQVSINNPNIDLVGIDRVENPNYIFINLTVKPDAKPGSIDIKFTFPKGK
                     PLTYAYELKARQVGSANRKGFDNSDVVYLVYPDRFSNGNPSIDQVSGYTDKVDRNEPY
                     ARHGGDIQGILNHLDYFKQLGVTALWLNPVLESNQPSWSYHGYAITDFYRVDPRLGSN
                     ELYREFIDKAHAQGIKVVKDMVFNHCGGEHWWMKDVPMKSWFNLYPDFKRTNYRIPTT
                     FDPHASVADSSLMADGWFDGHMPDLNQRNPYLANYLIQNSIWWIEYAGLDGIRMDTHP
                     YCDKHFMARWCDAVQHEYPNFSIVGETWVNYPAWVAYWQKDAKNRDGYNSNLEIVMDF
                     PLMFAMHKAFDEKEGWDTGLARLYEIIAHDFVYPNPDKLFIFADNHDIGRFQRDSSQA
                     LGRLKLAMAFLLTTRGIPQIYYGTEILLPGDDAKGHGDIRRDFPGGWPTDSKSAFTPE
                     GRTKRQNEIWNYMSTILNWRKTATAIHNGKLTHFIPENEVYVYFRHNSEQTVMVILHN
                     GYQPKVLDTSRFAEFLSNKTSGIDIISGNKVENLAKIQLSPRSAMIIELK"
     gene            complement(4747..5811)
                     /gene="fbaA"
                     /locus_tag="PLW22_08770"
     CDS             complement(4747..5811)
                     /gene="fbaA"
                     /locus_tag="PLW22_08770"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOU74836.1"
                     /translation="MKRFKPGVITGKELQELFAIAKAEQFALPAVNVTGTNTMNAVLE
                     TARELGSPVIIQFSNSGASFIAGKSLSNDGQQASILGAISGAKHVHLLAEAYGVPVVL
                     HTDHAAKKLLPWIDGLLDAGEKHFRETGKPLFSSHMLDLSEESLHENIEICKRYLERM
                     AKMGMTLEIELGVTGGEEDGVDNTGVDSSRLYTQPEEVAYAYEELMKISPNFTIAASF
                     GNVHGVYKPGNVVLNPIILKNSQELIKQKYATADKPVNFVFHGGSGSEKEKITEAISY
                     GVIKMNIDTDLQWAFWEGIKNYYEAKKGYLQGQIGNPEGEDAPNKKYYDPRVWLRKGE
                     ESIVARLKEAFADLNALGRG"
     gene            complement(5861..8185)
                     /locus_tag="PLW22_08775"
     CDS             complement(5861..8185)
                     /locus_tag="PLW22_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HOU74837.1"
                     /translation="MNPYLKHDEWCIIEEGFIPENTRASESIFSIGNGAMGQRANFEE
                     KFTGSTLQGSYIGGVYYPDKTRVGWWKNGYPEYFAKVINSTNWIGINVIVNGEELDLA
                     KCKVQDFRRVLNMKEGYLERSFIAELQNGIKLAVKSTRFLSMVDSELGAIRYSVKPLN
                     ADANIEVHPYLDADVHNEDSNYGEKFWEVVDIKGTSAFSYIVSRTKKLDFHVGHAMRN
                     FIYVNGQHQNVQPQVNTSAMYVDNRFTINAKAGDEVTLYKYAAVISSLNHEKGTFPKV
                     LEKLIDTAVSKGFDKMINEQRNAWAEIWQHSDITIEGDVAAQQGIRFNIFQLSQTYTG
                     KDERLNIGPKGFTGEKYGGSTYWDTEAYCLPFYMVTHGQKVARQLLLYRYKQLGKAIE
                     NAQKLGFTDGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAFAIYNYVRYTGDKSYL
                     VNFGLEVLIAIARFWAQRVNFSQEKGKYVMLGVTGPNEYENNVNNNWYTSTIACWCME
                     YAEEVASYVKSTDPSKYAELTKSIHFDESKEIAKWRDIRNNMFFPVDEKLGIFLQQEG
                     YMDKEQILVKDLDPKERPLNQKWSWDRILRSCFIKQADVLQGLYFLEDRYDTDTIKRN
                     YEFYEARTVHESSLSPCVHSVLASRIGNVDKAYELYLRTARLDLDDYNREVKEGLHIT
                     SMAGTWISIVEGFGGMRVRNNMLTFNPLIPDKWKSYSFKVWFRDNILKIMVTSSQITI
                     NNESGNAITLKIYGVEHSVPASSSISVPVQKQIF"
     gene            complement(8191..8853)
                     /gene="pgmB"
                     /locus_tag="PLW22_08780"
     CDS             complement(8191..8853)
                     /gene="pgmB"
                     /locus_tag="PLW22_08780"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOU74838.1"
                     /translation="MNEIKACIFDLDGVVVDTAKYHYLAWKRLAQELGFEFTEQHNER
                     LKGVSRMRSLEILLEVGGLSFPLDEMERMAAKKNAWYVEYIERMTPDEILPGAKELLE
                     ELRANGIKVSLGSASKNSPLILERIGLKNHFDAVIDGNSVTVAKPAPDIFLVAAKSLG
                     VNPEFCVVFEDAEAGIEAAINAGMRTVGIGNPQILNRADVVVPNLKGFTYSKLIELFL
                     KK"
     gene            complement(8858..10186)
                     /locus_tag="PLW22_08785"
     CDS             complement(8858..10186)
                     /locus_tag="PLW22_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU74839.1"
                     /translation="MAKKPKLSFWQIWNLSFGFLGVQAGFALQNANVSRILSNYGADL
                     HHLSFFWLVAPIMGLIIQPWVGAASDRTWTRMGRRKPFIFGGAIAASLGMFFMPNSHI
                     AIAILPPVIFGAIMLALMDASFNVTFQPFRSLVADMTPEEQTNTGYSIQTLLINLGAV
                     IGSFLPFVLTNLLGVGNTAPEGQVPPSVVWSFYIGGSILLISVLWTVFTTKEYNPDEF
                     KQYNQSDSVVVEAEKKSFWQTLTSMPGIMGKLAIVQFFSWFALFAMWVYTTPAVAQHI
                     WGTAIGDSTSPEYNEAANWVGVLFGLYSVFAAIFAIFLDKIANRLGRVKTYSISLLFG
                     ALGYLSMYIFTGQYALILSMVGVGIAWAAILAMPYAILARSLPADRMGVYMGIFNITV
                     VVPQIVSGLLLGTILKFVFNEKAIYMIVLAGLSMLLASASAILFVKEKKA"
BASE COUNT         3079 a         2355 c         2335 g         3023 t
ORIGIN      
        1 agcaggtaga gcacatccct tttaaggatg gggtcctggg ttcgaatccc agctgaatca
       61 ccaaaaagcc cgatggaaac gtcgggcttt tgtattaagg ttaaaggtta aaggttaaag
      121 gttaaagata aaaaggggta aaagttaaag agagcctctt ccgggaattt atttgatttt
      181 gcatctaatg taaatcaaac ctttggctat tgttgataca attataataa acacaaaaga
      241 agtatcgtaa agcaaaatcc tatacacaac cttttgctct ccaaaagtaa aaaaggtcgg
      301 attattgcta atccgaccat tttattggtt agaaattcat tagcttaatt tattctggtt
      361 gtgaatattt cgaaactgtt tggcttaagc gtaacttcaa gtttcccgtt ttggattgag
      421 tacttagaat tgaagtttgg atataatacg ctatcactca tatattcagg aagttgtagg
      481 tcgataactt gttcctggtt cgacttgttg aagattacaa ctacggtatt gtcaaaatac
      541 ttacgaacgt aagcgtaaac attatcgttt acgaggatgg tttggaatga gccgtaaacc
      601 agagccaggt tatttctgcg gatgtttaca agctttgtaa cagtttcaag ggtttgttgc
      661 tcaagggttg ataatccttc aaacttcatc atgcggcggt tgtcggggtc gttgccaccg
      721 ggcattccaa actcgtcgcc gtagtatatg gttggaacac cgggaatggt catgttgaat
      781 gcgttaagca tgcagagttt agcataagca gtactatcgc caacgccaat ttcgcgggtc
      841 caaccagcct ttttggcatc ctcaccgaaa cgtaaggctc caccggcata ggaaataaaa
      901 cgacccctat cctgatttcc tgagatatag cccataaggt taatccagcc aaaatagctg
      961 aatgtttggt gcagtgctcc atcaagctta gtaaaggggt agtcgttgcg ggcaaaagcg
     1021 gctaccgagg catcgtaaat gttaaaatcg aactgggcgt cgagcatgcc gctgccaatg
     1081 tagcttgcaa tgagttcgtg gctgccatag gtttcgccaa tctggtaaac cggtttattg
     1141 ggtaggttaa gcttgatttt ttgggtaagc ctacgccaga aattctcgtg aatatgttta
     1201 gtggcatcgt ggcggaatcc atcgaggtcg taatgggaaa cccagaataa agctgagtcg
     1261 gtcatgggtt cataaacctc aggacgttcc aggtcgagcg ttggaagaaa cgtatcgaac
     1321 caagttgtaa ggcgatactc atcccagcgt tcggtgttta gtgaaccatc gggcaggtaa
     1381 agcgatgtag cccagtcggg atgctgcttg taaaggggat gctcctggtg aacgtggttg
     1441 gccacgtaat cgagtataac gttcatgttt cgcttatggg cttcagcaat aagctcatta
     1501 agttcactct cagtcccaaa cctaaagtcg accttggaag aagaaaccgg ccagtagcca
     1561 tggtagcccg aaaatttggt gcggggttgt ggatataatc cccatgcccc taaggggttt
     1621 tgagtaattg gtgaaagcca tatagtattg attcccagtt tggtaaaata tccatcctta
     1681 accttttggg taacgccagc caggtcgcca ccaaagtaat ttgccttggg gtgtatttcg
     1741 ggatcgttca ccttgaaatc gttggatggg ttgccgttct taaagcggtc aaccaaaagg
     1801 aaatacatga tttgagcatg cttatcgcta cgggaaagct gcgacggatt tataaccacc
     1861 ctatttccat caagcggtat aagtagattg ttcgattccc cttgctggtt gtaggcccaa
     1921 atgcgtatat gggatcgctt gtaacctcgg gcattttcag gtattgttat ttcgtaaaat
     1981 tcttccttag aggtaataaa tttatcatcc attaagtaat tctgccacag aacaataaac
     2041 ttatctacat ttcctatcgc tttaattaac aatttatttc catccgttga gaatggaacg
     2101 agtttgggta gctcctcagg atttgtattg ccaacaacta acactgagtt atacccaccc
     2161 attccattat cttcaaaagt ggggtttgct gggtcaagca tccacttgcc gtcaataaca
     2221 acctggtaat ggtacttccc tggatttaaa agcttagtag ttttataaac ctttccatcg
     2281 aatgtaagat ttgtagaact gggattccag tcgtttattt gccctgcaag ctgaacgctt
     2341 tttacctttt tcccctgagg atcgaaggtg aaggttacag gaagtttacg atttttccga
     2401 accaataaac agtatttatc gcccttagcc cagataaaaa tgtatgagag ccatggcaat
     2461 gactgattgt cagctttcaa agtaaggtat gccttactat cggaaatgct atactgaaaa
     2521 tgtgagggta tagatacgga atcaatcgcc tgaggatcag ggaagtaatc ggttaggtaa
     2581 accaatgtgc tgtcgccagc aagctgaatt actgaaagca aatcatgaac tgccgggctg
     2641 gtagtagcat gttcaatccg taaacttttt tggcatgaac ttactaaaaa ggttacggta
     2701 atcaatgcta ataatctgta tttcatatac ataatatttt aaagatttac ttcaactcaa
     2761 ttatcatagc cgagcggggc gaaagctgaa tctttgcaag gttttcaacc ttatttccgg
     2821 aaataatatc aattccgctc gttttgttgg aaaggaactc agcaaagcga gaggtatcca
     2881 aaaccttggg ttgataaccg ttatgaagaa taaccatcac ggtttgctct gagttatggc
     2941 ggaaataaac atatacctca ttctcaggga taaaatgggt taattttccg ttgtgaatgg
     3001 cagtagccgt tttgcgccaa ttcaggatgg tagacatgta gttccagatt tcattttggc
     3061 gctttgttct accctcaggg gtaaaagccg acttggaatc ggttggccat ccgccgggaa
     3121 aatcgcggcg aatatcgcca tgccccttgg catcatcgcc gggcaataaa atctcagttc
     3181 cgtagtaaat ttgaggaatg ccacgtgtgg taagcaggaa tgccatggcg agctttaaac
     3241 ggcctaatgc ctgagaagaa tcgcgctgga aacggccgat atcgtgatta tcggcaaaga
     3301 tgaaaagctt atcgggattt gggtaaacaa agtcgtgggc aataatctcg taaaggcgag
     3361 ccagtcccgt atcccaaccc tccttctcat caaatgcctt gtgcatggca aacatcaggg
     3421 ggaaatccat tacaatctct aggttcgagt tatagccatc gcggtttttg gcatcctttt
     3481 gccagtatgc cacccaagca ggatagttta cccaggtttc gccaacaatg ctgaagttgg
     3541 gatattcatg ctggactgca tcgcaccagc gggccatgaa atgcttatcg caatacggat
     3601 gggtatccat acggatacca tccaacccgg catactcaat ccaccagatg ctgttctgta
     3661 ttaggtagtt ggcaaggtat gggtttcgtt ggttaaggtc gggcatgtgg ccgtcgaacc
     3721 aaccgtcggc cattaggctg ctatcggcca ctgacgcatg cgggtcgaaa gtggtaggaa
     3781 tgcggtagtt tgtccgtttg aaatcggggt aaaggttgaa ccagcttttc attggcacat
     3841 ccttcatcca ccagtgctca cccccacagt ggttgaaaac catatcctta actaccttga
     3901 tgccctgggc gtgtgcttta tcaatgaatt ccctgtaaag ctcattgcta cccaggcggg
     3961 ggtcaaccct gtaaaaatcg gttatggcat agccatggta cgaccacgaa ggctggttgc
     4021 tctcgagcac aggatttaac caaagcgctg ttactcccag ctgtttaaaa tagtcgaggt
     4081 gattcagaat accctgaata tcgccgccgt gacgggcgta aggttcattg cggtcaacct
     4141 tatcagtata tccgctaact tggtcaatgg aagggtttcc gttggaaaac ctgtcggggt
     4201 aaaccaggta aaccacatcg gaattatcaa agcctttgcg gttggctgag ccaacttgac
     4261 gggctttaag ttcgtaagca taagtaagtg gtttgccttt aggaaaggta aatttaatat
     4321 ctatactccc tggcttggca tcgggtttaa cggttaggtt gatgaaaata tagtttggat
     4381 tttcaaccct atcgatacct acaagatcga tattggggtt gttgatagaa acctgtgccg
     4441 aggcaatatt tttcccgtaa accagaagct gaaggtttgg attattcata ccaacccacc
     4501 agcaaggggg ttcgatacgc tcaaccggtt tttgagcaag tgatgtcatc accataaaca
     4561 tagtagacat cagtaaagat atgcggtttt taaacatggt tattggtttt agagtgattt
     4621 ttcctgaaaa caacaagggc tgtcagggaa gcccccaaca gcccaaattc taataaataa
     4681 ctatttggat atgaatactt ttattaccag ttggataaaa atccggaata tggaacaagc
     4741 tattcattac ccacgaccta aagcattaag atcggcaaaa gcctccttga gacgggcaac
     4801 aattgactct tcacctttgc gaagccaaac gcgaggatcg taatacttct tgttgggagc
     4861 atcttcccct tcgggattcc caatttgacc ctgcaggtaa cccttcttgg cctcgtaata
     4921 gtttttgatg ccttcccaga aagcccactg caaatcggta tcaatattca tctttattac
     4981 tccatagctg atggcctcag taattttttc cttttcggaa ccgctaccac catggaatac
     5041 aaagtttaca ggcttatcgg ctgtagcata tttctgctta attagctcct gtgagttctt
     5101 aaggataatt gggttaagaa ccacattgcc gggcttgtaa acgccatgca catttccaaa
     5161 cgatgctgct atggtgaaat tggggctaat tttcatcagc tcctcatatg cgtaagccac
     5221 ttcctcgggt tgggtgtata atcttgagct gtcaacacca gtattatcaa cgccatcctc
     5281 ctcgccgcca gtaacgccaa gctcaatttc cagggtcatt cccattttgg ccattcgctc
     5341 caggtaacgc ttgcagattt cgatattttc atgaagcgat tcctcggaaa gatcgagcat
     5401 gtgtgagctg aaaagaggtt ttcctgtttc gcggaaatgt ttttcgcccg catcgagcaa
     5461 gccatcaatc catggaagaa gttttttggc agcgtggtcg gtgtgaagta caacgggaac
     5521 accataggcc tcggccagca aatgaacatg cttagctccg cttattgccc caagtatact
     5581 agcctgttga ccgtcgttgg atagcgattt gcctgcaata aacgatgccc cgctgtttga
     5641 aaactgaata ataaccggtg agcccaactc acgggcggtt tccagcacgg cattcattgt
     5701 attagttcca gtaacgttaa ctgcaggtag agcaaactgc tcagccttag caattgcaaa
     5761 tagttcctgt aactcttttc cggttattac gcctggttta aaacgtttca tgattttgta
     5821 gttttaagaa atacaacgca aaggtaatct tttttcaggc ttagaaaatt tgtttctgaa
     5881 ccggaacgct tattgagctg gaggcaggaa ctgaatgctc aacaccgtaa atcttaaggg
     5941 ttattgcatt cccgctttca ttgttaatgg ttatttgtga actggttacc attatcttaa
     6001 gaatgttatc gcggaaccaa accttaaagc tatacgattt ccacttatcg ggaataagcg
     6061 ggttaaaagt gagcatgttg tttcgaacgc gcattccacc aaaaccttca acaatggaaa
     6121 tccaggtgcc ggccatactg gtaatgtgga gtccctcctt aacctcgcgg ttgtaatcgt
     6181 cgagatcgag gcgagctgtt cgtaggtaaa gctcataagc tttatcaaca ttgcctatac
     6241 gagatgccag aacgctatga acgcatggtg aaagcgatga ttcatgaact gttcgggcct
     6301 cgtaaaactc ataattacgt ttaatggtat cggtatcgta cctgtcctca aggaagtata
     6361 atccctgtaa aacatcggct tgcttaatga agcaggaacg cagaatacga tcccaactcc
     6421 atttctgatt taacggacgc tccttgggat caagatcctt aacaagaatc tgctccttgt
     6481 ccatataccc ttcctgttgc aggaatattc ctaacttttc atcaacaggg aagaacatat
     6541 tgttacggat atcgcgccac ttggctatct ccttagattc atcaaaatgg attgatttag
     6601 tcaactcagc atacttggat ggatcggttg atttaacgta agatgctacc tcctcagcat
     6661 actccatgca ccagcaagca atggttgaag tgtaccagtt gttattaacg ttattctcat
     6721 actcattagg gccggtaaca cccagcataa cgtacttacc cttttcctgc gagaagttca
     6781 cacgctgtgc ccaaaaacga gcaattgcaa tgagcacttc aaggccaaag tttaccaagt
     6841 aacttttatc gcctgtatag cgaacgtaat tgtaaattgc aaaagcaatg gcaccattac
     6901 gatgaatctc ctcaaaggta atttcccact cgttgtggca ctcctcaccg ttcatggtaa
     6961 ccattggata aagggctgca ccatcggtaa agccaagttt ttgtgcattt tcaatagcct
     7021 ttccaagttg cttgtagcgg tagagcagaa gttgacgggc aaccttttga ccgtgtgtaa
     7081 ccatatagaa aggtaaacag tatgcctcgg tatcccaata ggttgatcca ccatactttt
     7141 cacctgtaaa gccttttggg ccaatgttca agcgttcatc cttgcccgta taggtttggc
     7201 tgagctggaa aatattaaag cggataccct gctgagcagc cacatcgccc tcaatggtga
     7261 tatcgctatg ttgccaaatc tcagcccatg cattacgctg ctcgttaatc attttgtcga
     7321 aaccttttga tacggcagta tcaataagct tttcgagaac cttagggaat gttccctttt
     7381 catggttcag gcttgagatt acagcagcat acttgtagag tgttacttca tcgccagcct
     7441 ttgcattaat ggtaaacctg ttgtcgacat acatagccga ggtgtttact tgcggctgaa
     7501 cattctgatg ctgaccgtta acgtaaataa agttacgcat ggcatggcca acgtggaaat
     7561 cgagcttttt agtacggctt acaatgtagc tgaaagctga tgtgccttta atatcgacca
     7621 cttcccagaa cttctcgcca tagttggaat cctcattgtg aacatcggca tcgaggtatg
     7681 ggtgaacctc gatattagca tcggcattaa gcggtttaac agagtaacgt atagcgccaa
     7741 gttcagaatc gaccatactt aaaaaccgtg ttgacttaac ggctaacttt ataccgttct
     7801 gtagctcggc aataaatgaa cgttcaaggt atccctcctt catgttgagt accctacgga
     7861 aatcctgaac tttacacttg gccaggtcga gctcctcgcc gtttacaatt acattgattc
     7921 caatccagtt ggtagagttg ataaccttgg cgaaatactc agggtagcca tttttccacc
     7981 aacccactcg ggttttatcg gggtagtaaa cgccacctat gtagcttccc tgaagcgtgc
     8041 tgccggtgaa tttctcttca aagttagcac gctgtcccat agcaccattt ccaatgctga
     8101 aaatactctc cgaagcgcga gtattttcag gaataaatcc ctcttcaatg atgcaccact
     8161 cgtcgtgttt aagataaggg ttcatatcga ttactttttt aagaatagtt caattaattt
     8221 gctatatgta aaacctttaa gattaggcac aaccacatcg gcacggttca gaatttgagg
     8281 attgccaata ccaacagtgc gcatacctgc attaattgca gcctcaatac cggcttcggc
     8341 atcctcaaat acaacacaaa actcaggatt taccccaagc gattttgcag caaccaggaa
     8401 gatatcaggt gccggtttgg ctactgtaac cgagttacca tcaattacag catcgaaatg
     8461 attttttaaa ccaatccgtt caaggataag gggcgaattt ttgcttgcag aaccaagaga
     8521 aacttttatg ccattagccc ttaactcttc cagcaattcc tttgcgccag gaagaatctc
     8581 atcgggggtc attcgttcaa tgtactccac gtaccaggca tttttcttgg cagccattcg
     8641 ttccatttca tccaatggaa acgacaaacc accaacctcc agcaaaattt caagggagcg
     8701 catacggcta actcctttaa ggcgctcgtt atgctgctcg gtaaactcaa agcccaactc
     8761 ctgcgcaagg cgcttccaag ccaagtagtg atactttgca gtatcgacca ctaccccatc
     8821 taaatcgaaa atacaagcct taatctcatt catcgggtta agcctttttc tctttaacaa
     8881 aaagaatagc gcttgccgaa gctaaaagca tcgataaacc agcaagaact atcatataga
     8941 ttgccttttc gttgaaaaca aatttgagaa tagttccaag cagtaaacca cttacaattt
     9001 gcggtactac cacggttatg ttgaaaatgc ccatatacac tcccattcta tcggctggta
     9061 gcgaacgcgc aagaattgcg taaggcatag ccagaatggc agcccaagca attccaacac
     9121 ctaccattga aagaataaga gcatattgac cggtaaatat gtacatcgat aaatagccta
     9181 atgcaccaaa aagaagcgaa atggaatagg tttttactct acccaagcga ttagctatct
     9241 tgtcgaggaa aatggcaaaa atggccgcaa aaacgctata taatccaaaa agaaccccta
     9301 cccagttagc cgcttcgtta tactcaggag aggtggaatc gccaatggct gttccccaaa
     9361 tatgctgggc aacggctggt gttgtataaa cccacatggc aaacagagca aaccaagaaa
     9421 agaactgaac aatggctagt ttgcccatta tacccggcat tgatgttaaa gtttgccaga
     9481 aacttttttt ctcagcttca acaacaacgc tatcggattg gttgtactgt ttaaactcat
     9541 cagggttata ttccttggtt gtgaacacag tccacaaaac cgatatcaac agaatagaac
     9601 caccaatata aaacgaccaa accacggagg ggggaacttg tccctcagga gcggtattac
     9661 ccactccaag caaattagtg aggacgaaag gtaggaaaga accaataacg gcacccagat
     9721 tgataagcag ggtttgaatt gaataaccag tattggtttg ctcttcgggg gtcatgtcgg
     9781 caaccaaaga gcgaaaaggc tggaaagtaa cgttgaacga agcatccatt aatgccagca
     9841 ttattgctcc aaaaattaca ggaggtagaa tggctattgc aatatgtgag ttaggcataa
     9901 aaaacattcc taatgaagcc gcaatagcac ctccaaaaat aaaaggttta cggcgcccca
     9961 ttctagtcca tgtcctatcg cttgctgctc ctacccatgg ctgaatgatt agccccatga
    10021 ttggagctac aagccagaaa aaagagaggt gatgaaggtc agcaccatag tttgatagta
    10081 tgcgtgaaac attggcattt tgcagggcaa aacctgcctg aacccctaaa aagccaaagc
    10141 taaggttcca aatttgccaa aagctgagtt taggtttctt tgccataaag ctatttttag
    10201 ttttcaaggt gtccaaaatt aaagtaaaaa aatcttaaaa aaaacataac ataaagttaa
    10261 aacccttatg aaataagggt ttcagaacat gaaagaggca tttcaatcgt ttacgaaggg
    10321 ttgaagaaat atttcaaaaa ttttcattac ggaaaccctt gaacatcagg aaaaccgcgt
    10381 aaaatcaacc gtaaacgttt gcggtaaatt tatcgatggg tgtgttttga tattgaattt
    10441 aaattgaacg cagatgacgc agattgtgtg gatttactct gtgaaacgat taaagttgat
    10501 ggttgttagt tgattgttga ttgttgatag tttggtgctg cctcgtctta tcctgtcatg
    10561 ctgagcgcaa ccgaagcatc actatgtgct atcatccctt atttaatgag attcctcgac
    10621 aagctcggaa tgacaaaaaa catacttctt ctactccatt gtcatgctga gcaccgccga
    10681 agcatctctt tttacctcca attcatttat accttttgat taggttccaa gcagtcactt
    10741 ttgggaatgt ttttcctctt gcgatgcaac gtttcaccct tgctctttgg aa
//
LOCUS       DAOOWJ010000088        10753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6120_length_10753_cov_7.740606, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000088 DAOOWJ010000000
VERSION     DAOOWJ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_10753_cov_7.740606"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..847
                     /locus_tag="PLW22_08790"
     CDS             401..847
                     /locus_tag="PLW22_08790"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOU74840.1"
                     /translation="MKIKYEMLRKLTVYLMSVLLLLASKADTLVYQDSPEAIVKKSAE
                     AIEKGDVENLTRNFFKTVEIDLLGDENFYSQAQSVLLLRSFFEKNIPVKFTINHQGAK
                     DLTAFAIGTLQCKNGLFRISIFLKTDQGKTYIHQFRIEPESAINPK"
     gene            852..1703
                     /gene="nadC"
                     /locus_tag="PLW22_08795"
     CDS             852..1703
                     /gene="nadC"
                     /locus_tag="PLW22_08795"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOU74841.1"
                     /translation="MNTYDNLIDRLIDLAIAEDLGDGDHSSLSCIPYNAAGVVQLMMK
                     QEGVIAGLRIAERIYKRIESDVQVDFLKTDGEFLQPGDIAFRAYGRVHALLQAERIVL
                     NVLQRMSGVATQTREYVKLCEGTKAKVLDTRKTTPGMRVLDKMAVAIGGGLNHRMGLF
                     DMIMLKDNHIDFAGGIQEAIRMASEYLAKTGRNLDIEVETRSLKDVETVLRVGGVRRI
                     MLDNFTVADTRKAVELINGRFETESSGGINLSTIRDYALCGVDYISVGAITHQIKSID
                     MNLKKID"
     gene            1797..2669
                     /locus_tag="PLW22_08800"
     CDS             1797..2669
                     /locus_tag="PLW22_08800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOU74842.1"
                     /translation="MPSLHGVPVVDVIRYFFRGIVNGAITTRASAVAFSFFLATVPLL
                     IFIFTLIPYLPLENFQFQLLQLAKGIMPTYAYQTVEATLVEVVTTKSSGLLSFGFLAA
                     IFFSHNGVSALIDAFNATYHTIETRSFVEQHLIALLLTFLLPSIVILGIVLLFFGETL
                     LNILGDWGVLHSSSILFIAMIIKWLLAVLVFFFGISLVYYLAPSRKDKFSFFSPGAIL
                     ATVLIILTSFAFSYYVNRFGQYNKFYGSLGGLIAFQLWLYFNAFGLILGFDLNASIRS
                     AINGRRQRIDIQMD"
     assembly_gap    2733..2832
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2833..3060
                     /locus_tag="PLW22_08805"
     CDS             <2833..3060
                     /locus_tag="PLW22_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU74843.1"
                     /translation="SPQSTVIPNRKYLERLMADFEEEFKGKTVKRPPNWGGYTLVPTL
                     FEFWQGRPNRLHDRIQYTLINNQWKIERLAP"
     gene            complement(3057..6854)
                     /locus_tag="PLW22_08810"
     CDS             complement(3057..6854)
                     /locus_tag="PLW22_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU74844.1"
                     /translation="MASKVALLIINQILPVDKFETILEAKGYSCLTLDRISSDIELPI
                     FNIAIVGLDNEKTYLKLKEKTDDYDGVILFFSGKHYNEIASQIAKLPESLHIEPNFSG
                     KELELAIQLQLLKKQIQKAESFASSNFDTNHVRLKKIITEEQAWRIIFEQSPNGVLLS
                     DDEGNIIYTNSAATDILGYTRDEFLKMRFHDLAPKELAEQVDTNIKKILRGESLITEV
                     YNIRKDGSKRYVQLHETRVIFPNGKYGIMVISTDISRAKQAEEALKESMEMYRILVEH
                     SNDGIVFVKEGIVIYGNPRIIEILNAKPEEFLNQPITKFIHPSQKEFLLQRYKNRLAG
                     KSEPTIYETILVSSQNEEINVEFNVNLVDQAGEKIDIVFIRDITTRKQAEKAIKESEE
                     SYKGLFNNSSEGILIIDSSGVILDANRSGQILFGYSKDELLGKTIHEITDNTQTSLEE
                     VTRSVRLAYQGHTQKMEFWGIRKNNDLVPIDMVFNRGDYFGLSVVFAMCHDITDRKNA
                     EAVLRESEEKYRTLTEQIPVGLYRILSNGQIVYCNPAFAEIFGFSSSQAATGSNIKSF
                     LRDFDKWLVDCSTAKLPIEVEMLRADGSTIWVQNILQPNVTENPDATYFDGMLTDVTD
                     RKVAIDELTESEAKFRAMLLAIPDHLFRLNREGILIDFAPTDTAFYPEITNLHLGKNI
                     AEIFPSSIYEKYSEALSYASITGKLQRFEYSFKQENSIFYYEARIVPAGEKIFLVLLR
                     DITQQKKFEEEIRILAQTIMNANDSISITDLDGNFIYVNPAFTKVYGYSQTEILGKNI
                     NLIRPIGFDSDLSKEIHSATQKGGWKGEILNVRKDGTIFPIFLSTSPVYNEFNEPIAS
                     VGIANDITERKLIEQELIRAKEQAEESDRLKTAFLSNMSHEIRSPMNAVLGFIQLLKA
                     EESITETGKQYIELIENSGNQLLALIEDIIDISKIQSNQIRINKTSFDFNELLRELYT
                     IYSNQLIRKADQKTLLFKPEMPQESPFTIYSDPVRVRQILNNLLSNAIKFTPAGHIRF
                     GYTLMIDEDMPYIQCYVQDTGIGISPEKQALIFERFRQADDTYTRIYGGSGLGLAISK
                     GLVDLLGGHIWVESTEGQGSTFYFTIPFQEGTESGKSLKKEEQKTLSSELLKGKKILV
                     VEDISEIRLYFERLLEPTGAKTLFAHNAREARVLFKKHKDISVVLLDIRLPDADGYTI
                     AAEFKRLRPDLPIIAQTAYALQSEFNKSIESGCDDYVTKPIDSPLLIQKILSLIEKKS
                     DNH"
     gene            complement(6991..7461)
                     /locus_tag="PLW22_08815"
     CDS             complement(6991..7461)
                     /locus_tag="PLW22_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74845.1"
                     /translation="MIIIEIPGRKTLRIEHLVLDFNGTLAIDGKLIEGVRERLQQLST
                     QLMVHVITADTFGTATHELRGIDCKVKVLEKGRQDKQKKIYINELGKNKCATIGNGYN
                     DWQMLKHAALSIATIQAEGASTLSLKQASIVCYNVNHALDLLLNPNRLVATLRR"
     gene            complement(7468..7935)
                     /locus_tag="PLW22_08820"
     CDS             complement(7468..7935)
                     /locus_tag="PLW22_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOU74846.1"
                     /translation="MEIERKFLVDLPKWEKLDKPNPIRIVQGYLSTNPEQTIRVRVWA
                     DKAFMTIKGKVQGISREEIEFEIPTGKANELLTKFCKAVIKKNRYLIVHNSKTWEVDV
                     FEGENAGLVLAEIELGSEDEKFTVPHWITIEVTDDYRYYNSYLSENPYSSWKK"
     gene            complement(7993..9051)
                     /gene="arsB"
                     /locus_tag="PLW22_08825"
     CDS             complement(7993..9051)
                     /gene="arsB"
                     /locus_tag="PLW22_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOU74847.1"
                     /translation="MKKRLKFLDRYLTLWIFLAMAIGVLSGHFFPGIADFWDSMKPSP
                     ESTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNYRILSLSLVQNWLIGPVLMFALAI
                     TFYKLFPSETNLQYMYGIIMIGLARCIAMVIVWNDLAKGDTEYAAGLVAFNSIFQVLF
                     YSVYAYVFIAILPGWFGIPTNNSVEQITIGQIAESVFIYLGIPFIAGFITRFTLVRLK
                     GDNWFNTKFIPKISPLTLIALLFTIFVMFSLKGEYIVKIPMDVVLIAIPLLAYFVIMF
                     LVSFFMSRKAGATYEQTTTLSFTAASNNFELAIAVAIAVFGISSGEAFAAVIGPLVEV
                     PVMIGLVNVAFYFKKRFF"
     gene            complement(9055..9468)
                     /locus_tag="PLW22_08830"
     CDS             complement(9055..9468)
                     /locus_tag="PLW22_08830"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOU74848.1"
                     /translation="MTRILILCTGNSCRSQMAEGFLKSFDKQLTVESAGTEPAAQVNP
                     NAVKVMAEVGIDISKNKPKLVDQFIGQEWDYVITVCDNAKETCPAFLGKVKHRLHIGF
                     EDPAEAKGTPEEVMAVFRKVRDEIKDRFSEFTKTI"
     gene            complement(9482..9898)
                     /locus_tag="PLW22_08835"
     CDS             complement(9482..9898)
                     /locus_tag="PLW22_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOU74849.1"
                     /translation="MEANPFSGNGFMSGGFLNLTPRDAYYEAKTGKAIIVDVREENLT
                     GYKRFDVPKVIYLPLSELKQNLGLLPTDFPLVIADSTGLRSHEAMEILIARGFTLIAN
                     LAGGIVEWERDGLPLVINNKERLDGSCMCQLRPRFK"
     gene            complement(9911..10198)
                     /locus_tag="PLW22_08840"
     CDS             complement(9911..10198)
                     /locus_tag="PLW22_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74850.1"
                     /translation="MKLSVKAKEELLKVLETIDENSKGIRIYSTQGCCGPSVQMDVAS
                     APQPGETATSIDGVDFYIEDSLAETLAEVTLDHNGFGFLMNGLKKSGSCCG"
     gene            complement(10217..10546)
                     /locus_tag="PLW22_08845"
     CDS             complement(10217..10546)
                     /locus_tag="PLW22_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU74851.1"
                     /translation="MNKSEVFDEKLQELARFARAISHPARLAILQYLAETRTCISGDI
                     SQNLPLSRTTVSQHLKELKELGLIHGEIDGLRINYCLCSESIHKYLDEFDSFFGKVRS
                     ASVNCEI"
BASE COUNT         2832 a         2208 c         2284 g         3329 t
ORIGIN      
        1 taaacattta tttccgcaaa ggtaatagag ttttgagccg aattgccatg aattagttga
       61 ggaatgagtc ggaaagagtc ggaattgaat gaaaatgatg gagttagagg aagttaaaag
      121 aagtatgagg gaaagtgaac agttaaaccc aactaaaata aatcgggaca gttcgaaata
      181 attcggaata atgcggaatg attagatttt gttggtcgat tcgtcaattg atttttttat
      241 taaccagatt gcgtcaaatt attttgttta cactgattcc caatcttcat aaaaatactt
      301 aattttgaaa tgtgcatttt aatttgcagg ttgatattcg ctttctggct tataatttgt
      361 tgtttagcta ttctatattt tacctttggg gaattaaaag atgaaaatta agtacgagat
      421 gttgcggaaa ttaacagttt acttgatgtc tgtattgctg ttgcttgcat caaaagccga
      481 tactttagtt tatcaggatt cgccagaagc aatagttaag aagagtgccg aggcaattga
      541 aaagggcgat gtggaaaatc ttacccgaaa cttcttcaaa acagttgaaa tcgacttact
      601 tggcgatgaa aatttttata gtcaggcgca gtcagtgcta ctgctccgtt ctttttttga
      661 gaaaaatatt cctgtcaagt tcaccattaa ccatcagggt gctaaggatt taacagcatt
      721 tgccatagga actttgcagt gcaagaatgg attattccgc atatcgatat ttttaaagac
      781 cgatcaggga aaaacataca ttcatcagtt caggattgag cctgagtcgg ccataaatcc
      841 aaagtaagcc aatgaataca tacgataatc ttattgatag gctaattgac ttagccattg
      901 ctgaggactt aggcgatggc gatcactctt cactctcctg tataccatac aatgcggcgg
      961 gcgttgtgca gctcatgatg aagcaggagg gtgttattgc cggtttacgg attgctgagc
     1021 gaatatacaa acggattgaa agtgatgttc aggttgattt tttgaaaacc gatggtgagt
     1081 ttctccaacc cggcgatatt gcctttaggg cttatggcag ggttcatgca cttttgcagg
     1141 ccgaaagaat tgttcttaac gttctccagc gcatgagtgg tgttgccacc caaacccgag
     1201 agtatgttaa actttgcgag ggaacaaagg caaaagtgct cgatactcgt aaaaccacac
     1261 ccggtatgcg tgttctcgat aaaatggcgg ttgccattgg cggtggctta aatcacagaa
     1321 tgggactttt tgatatgatt atgcttaagg ataaccacat tgactttgca ggaggaattc
     1381 aggaggcaat tcgtatggca agcgaatatc ttgccaaaac cggccgaaac ctcgatattg
     1441 aggttgaaac ccgcagttta aaggatgtgg aaaccgtttt acgtgtaggc ggcgttcggc
     1501 gcatcatgct cgataatttt acggttgccg atacccgcaa ggctgttgag ctgattaatg
     1561 gccgtttcga aacggaatcg tcgggaggga taaacctcag caccatacgc gactacgccc
     1621 tttgcggtgt cgattacata tcggttgggg ctattaccca tcaaatcaaa agcatcgata
     1681 tgaatctaaa gaaaattgat taaggaatgg taaagccttt caggaaatat aaggcatcca
     1741 taaagcgctt taaaagcgca gcagctttgt tccgtcgaag gttaaaatat tttaccgtgc
     1801 caagcctaca tggggttccg gtagtagatg taattcgata ttttttccgc ggcattgtaa
     1861 atggtgctat tactacaagg gcatcggctg tggcctttag cttttttctg gcaacggttc
     1921 ccctgctaat ttttatcttc acgcttattc catacctacc acttgagaat tttcagtttc
     1981 agctgcttca gcttgcaaag gggattatgc caacctacgc ctaccaaacg gttgaggcaa
     2041 ctttggttga ggtggttacc actaagagct ccggtttact ttcgtttggt ttccttgcgg
     2101 ccatattttt cagccacaat ggtgtaagcg cccttattga tgcgttcaat gccacatacc
     2161 atacaattga aacccgtagc tttgttgagc aacacctaat tgcactgctt ttaacctttc
     2221 tgttgccatc aatagtgata cttggtattg tgctgctgtt ttttggggag accctattga
     2281 acattttggg cgactggggt gttttacatt catcgtcgat tttgtttatt gctatgatta
     2341 ttaagtggtt gcttgccgtg ctggttttct tttttggtat atcgctggtt tactaccttg
     2401 ccccttcgcg caaggacaag tttagctttt tctcacccgg agccatactt gcaaccgtac
     2461 tcataattct tacctccttt gccttttcat actatgtgaa ccgttttgga cagtataata
     2521 agttttatgg ttcactggga gggctaatag cttttcagct ttggctctac tttaatgcct
     2581 ttgggttgat acttggcttc gacctaaatg ccagtatcag aagcgccatt aatggtcgta
     2641 ggcagcgaat cgacatacag atggattaat tttaatgatg atgtcatgag ccctaaagac
     2701 cttaacgcac tcagacatga gtattccttg aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nntcgcctca gagcacagtg attcccaacc gcaagtacct ggaacgctta
     2881 atggccgatt ttgaggaaga atttaaaggg aaaaccgtaa aacgcccacc caactggggt
     2941 ggatacactc tagtacctac actttttgaa ttctggcagg ggcgtcctaa ccgtttacac
     3001 gataggatac agtacacact gattaataat caatggaaaa ttgagcgctt agctccttag
     3061 tgattatcgg atttcttttc tattaagctt aatatttttt ggatgagcag gggtgagtcg
     3121 atgggcttgg ttacgtaatc gtcgcagccg ctctctatac tcttgttaaa ctccgattgg
     3181 agtgcatatg ctgtttgggc aataatgggc aaatcggggc gcaggcgttt gaactccgca
     3241 gctattgtgt aaccatcggc atcgggaagc ctgatgtcga gcagaacaac gctaatatcc
     3301 ttatgctttt tgaatagcac tcgtgcctcg cgtgcattat gtgcaaaaag agtttttgca
     3361 ccggttggtt caagtaagcg ctcaaagtat agcctaatct cactgatatc ctccactact
     3421 aatatctttt tgccttttag caactctgac gatagggttt tttgttcctc tttttttaag
     3481 ctcttaccac tctcagtgcc ttcctggaaa ggaattgtga agtagaatgt tgagccttga
     3541 ccctcagtgc tttctaccca aatatgtcct cctagcaggt cgactaatcc tttggatatg
     3601 gctaatccta atccactacc accgtaaata cgggtatagg tatcgtcggc ttgccggaag
     3661 cgctcaaaaa taagggcctg tttttcgggc gatataccaa ttccagtatc ctgaacatag
     3721 cactgaatgt agggcatatc ttcatctatc atgagggtat atccaaatcg gatatggcca
     3781 gctggggtaa acttaatggc attgctcagt aaattgttaa ggatttgcct taccctcacg
     3841 ggatcggagt atatagtaaa tggtgattcc tgtggcattt cgggcttaaa caggagggtt
     3901 ttttgatcgg ctttccgtat gagctggttg gagtaaatgg tgtaaagttc ccgtaaaagc
     3961 tcgttaaaat cgaatgaagt tttgttgatt cttatttggt tggactgaat ctttgagata
     4021 tcaataatgt cttcaattaa ggcaagaagc tggtttccgc tattttcaat cagttcaatg
     4081 tattgctttc cggtttcagt aatactttcc tctgctttaa gcagttggat aaatccaagc
     4141 actgcattca tgggcgagcg gatttcgtgg ctcatgtttg agaggaacgc cgtttttaac
     4201 ctgtccgact cctcggcctg ttccttagcc cttataagct cctgttcaat taacttgcgc
     4261 tcagtaatgt cgttagcaat tcccaccgat gcaattggct cgttgaactc gttgtataca
     4321 ggtgaggtgg agaggaatat tgggaagatt gttccatcct ttcgcacatt aagaatctcg
     4381 cctttccatc cccctttctg ggtagctgaa tgaatctcct tgctgaggtc ggagtcgaaa
     4441 cctattggtc tgattaagtt aatgtttttt cccagtattt ccgtttggct atagccataa
     4501 actttagtga aggcaggatt gacgtatata aaattcccat cgaggtcagt gattgatatg
     4561 ctgtcgttgg cattcattat ggtttgtgcc agaatccgta tctcctcctc aaatttcttt
     4621 tgttgggtga tgtcgcgcag caaaaccaga aatatctttt caccggctgg aactatgcga
     4681 gcctcgtagt aaaatatgga attttcttgc ttgaacgagt actcaaacct ttgaagtttc
     4741 ccggttattg aagcatagct aagcgcctcg gagtattttt cgtatatgga tgatgggaaa
     4801 atttccgcta tgtttttgcc caggtgaagg ttagttattt ccggataaaa tgctgtatcg
     4861 gtgggggcaa agtcgataag gatgccttcg cggttaagcc ggaacagatg gtcaggtatt
     4921 gctaagagca tggctctgaa cttagcctcg ctttcagtaa gctcatctat ggcaacttta
     4981 cgatcggtta cgtctgttag cataccatca aagtatgtgg catcggggtt ttcggtaaca
     5041 ttgggctgga gaatattttg aacccatatg gtggagccgt cggccctcaa catttcaacc
     5101 tcaattggca acttggcagt tgagcagtca accaaccact tgtcaaaatc tctaaggaac
     5161 gatttaatat tactgccggt ggccgcttgg gaactactaa acccaaaaat ttcagcaaat
     5221 gcgggattgc agtaaacaat ttgcccgttg ctaagaattc ggtaaagacc cacaggaatc
     5281 tgctcagtaa gtgtacggta cttttcctcg ctttcgcgga gcactgcctc ggcatttttc
     5341 cggtcggtta tatcgtgaca catggcaaaa acaaccgaaa gcccaaagta gtcgcctcgg
     5401 ttaaaaacca tatcaatagg aacaagatca ttgttcttcc taatccccca aaattccatt
     5461 ttttgggtat gaccctggta tgcaagcctt accgaacggg taacctcctc gagcgatgtt
     5521 tgtgtattat cggtaatttc atgaattgtt tttccgagta gctcatcctt gctgtatccg
     5581 aagagtattt gtccgcttct attggcatca agaattacgc cgcttgagtc aataataaga
     5641 ataccttcgc tggagttgtt gaataatccc ttatagcttt cctcgctctc ttttatggcc
     5701 ttttcggcct gttttcttgt ggtgatatcg cggatgaaaa caatatcgat cttttcaccc
     5761 gcctgatcga ctaaatttac gttaaattca acattaattt cctcattctg gctcgacacg
     5821 agaatggttt cgtaaattgt tggctcagat ttacccgcaa ggcggttttt atatcgctgg
     5881 agcagaaatt ctttttggct tggatgtata aacttagtta ttggttggtt aaggaactct
     5941 tcgggtttgg cgtttagaat ctcaatgata cggggattcc cgtatattac gattccttcc
     6001 tttacaaata caataccatc gttggagtgt tcaactagaa tcctatacat ctccatcgat
     6061 tccttaaggg cctcttcggc ttgcttggct cggcttatat cggttgaaat aaccattatg
     6121 ccatacttcc cgtttggaaa aataaccctg gtttcgtgaa gttggacata gcgcttactg
     6181 ccgtcttttc gaatgttata gacctcagtg attagggatt cgcctcgcag tatctttttg
     6241 atattggtat ctacctgctc cgctagctcc ttgggcgcaa ggtcgtgaaa gcgcattttt
     6301 aggaactcgt cgcgagtgta gcctaaaata tcggtagcgg cagaattggt gtatattatg
     6361 ttcccttcat cgtcgctgag gagaacgccg ttaggcgatt gctcaaagat tattctccaa
     6421 gcctgttcct cggttataat ctttttaagc ctgacatggt ttgtgtcaaa gttgctgctg
     6481 gcaaaacttt cagctttttg tatctgcttt ttgagcagct gtagctgaat agcgagctct
     6541 agttctttcc ctgaaaaatt aggctcaatg tgtaaactct caggcagttt tgctatttgg
     6601 ctggcaattt cattgtaatg ttttccggaa aagaaaagta tcaccccgtc gtagtcgtca
     6661 gttttctctt ttagttttag gtacgttttt tcattgtcca gacctactat ggcaatatta
     6721 aagataggta gctcaatatc ggaggaaatt ctatccaagg ttaggcacga ataacccttt
     6781 gcttccagta ttgtttcaaa tttatcaacc ggaagaatct gattaataat aagtaacgca
     6841 accttcgatg ccaactccta ttagtttttc atgcgtgtaa tatatggtat ttttacgttt
     6901 attaacaaat ttaaggctcc tttttggtgt gttttttacc gaaaaggact aatggttgac
     6961 aaatgctgga gttggattga ttaattgcgt ttaccttcta agagtagcta ccaaacggtt
     7021 gggattaagt aaaagatcga gtgcgtggtt tacattgtag catacaattg aagcctgctt
     7081 cagggaaagc gttgaagcac cttctgcttg aattgttgca atactcaagg cggcatgctt
     7141 tagcatttgc caatcgttgt aaccattgcc aatagtggcg catttatttt tcccaagctc
     7201 atttatgtag atttttttct gcttgtcctg ccgccccttt tcaagtacct taactttaca
     7261 gtcaatacct cttagttcgt gggttgcagt accaaaggta tcggcggtaa tcacatgaac
     7321 catcagctga gttgataatt gctgcaagcg ttctcttacc ccttcaatca atttgccatc
     7381 aatggcaaga gtgccgttaa agtcgagcac caagtgctca atccgtagag tttttctacc
     7441 gggtatctca atgattatca tggtcaatca ttttttccag ctagagtatg ggttttcaga
     7501 tagatacgaa ttgtaatatc tataatcgtc ggttacttca atggttatcc agtggggtac
     7561 cgtaaatttt tcgtcctctg agccaagttc aatctctgca agtactagtc cagcgttttc
     7621 gccttcaaac acatcaacct cccaggtttt gctattatga acaattaggt atctgttttt
     7681 tttgataact gctttacaaa acttggtaag cagttcattg gccttaccgg taggtatttc
     7741 aaactcaatc tcctcgcggg atatcccttg aactttgcct ttaatggtca taaaggcttt
     7801 atcggcccaa actcttaccc ttatagtttg ctcagggttt gtgctaagat aaccctgtac
     7861 aattcttata gggtttggtt tgtctagttt ctcccattta gggagatcaa ccaaaaattt
     7921 acgctcaatt tccatacagt aaaaataaaa aaagcccgcc catatagcgg gccttatata
     7981 gctttttgtg ggttaaaaaa atcttttttt gaagtagaat gcaacattta caagtccaat
     8041 catcacagga acttcaacca gtgggccaat tacggcagca aaggcttcgc ctgaactgat
     8101 accaaaaaca gcaatggcaa cggctatggc aagctcaaaa ttgttgctag cagcagtaaa
     8161 ggagagagtg gtggtttgct cgtacgtggc acctgctttt cggctcatga agaaggatac
     8221 aaggaacatt attacgaaat atgcgagcag aggaatagca ataagcacaa catccatggg
     8281 gatttttact atgtattccc ctttaaggga gaacattaca aaaatcgtaa agagtaacgc
     8341 aattaaagtt aatgggctta ttttaggtat aaatttggtg ttaaaccaat tatcgccctt
     8401 taacctaact agggtaaaac gtgtaataaa accagcaatg aaaggtatcc caaggtaaat
     8461 aaaaacactt tcagctattt ggccaatggt aatttgttca acagagttgt tggtgggtat
     8521 tccaaaccag ccgggtagta tggctataaa tacataagcg tatactgagt aaaagagaac
     8581 ctgaaagata gagttaaaag caaccaagcc agccgcatac tcagtatcgc ctttggccaa
     8641 atcgttccaa acaataacca tagcaatgca acgtgcaagc cctatcatta tgatgccgta
     8701 catgtattgc aggttggttt cgctggggaa cagcttgtaa aaagttattg ctagtgcaaa
     8761 catgagtact ggccctatga gccaattctg aacaagcgaa agcgaaagaa ttctgtaatt
     8821 tcggaaaaca tcgccaagct cctcatactt tactttagca aggggcggat acatcattag
     8881 tattagtcca attgcaatgg gaatattggt tgtgctttcg ggcgaaggtt tcattgaatc
     8941 ccaaaaatca gctattcccg ggaaaaagtg gccacttagt acacctatgg ccattgccaa
     9001 aaaaatccac agggttaagt acctgtccag aaacttcaga cgttttttca tgaatcaaat
     9061 tgttttagta aactcactaa acctatcctt aatctcgtct ctaacttttc ggaataccgc
     9121 cattacctcc tcaggtgttc cttttgcttc ggccggatcc tcaaaaccaa tgtgaagcct
     9181 atgctttact ttacccagga atgccggaca ggtttccttg gcattatcgc aaaccgtaat
     9241 cacgtaatcc cattcctggc caataaactg gtcaaccagt ttgggtttgt ttttgctaat
     9301 atcaatgccc acctcggcca tcacctttac ggcgtttggg tttacctgtg cggccggttc
     9361 tgttccggcc gattcaacgg ttagctgttt atcgaacgat ttcagaaatc cttcagccat
     9421 ctggcttcga cagctgttgc ctgtgcataa aatcagtatt cttgtcattt tgatataaag
     9481 tttacttaaa ccttggcctt agctgacaca tacaggagcc atcgaggcgt tccttgttgt
     9541 tgattactaa tggaagccca tcgcgttccc actcaacaat accgccggca aggttagcaa
     9601 ttaatgtgaa acctctggca ataagtattt ccattgcttc gtggcttcgc aaccctgttg
     9661 agtcggcaat tactagcggg aaatcagtgg gaagtaaccc taggttctgc tttagctcac
     9721 ttaaaggtaa gtagataacc ttgggtacgt cgaagcgttt gtagcctgta aggttctctt
     9781 ccctaacatc aacaattatg gctttccccg ttttagcctc gtagtaagca tcgcgtgggg
     9841 ttaggttgag aaaacctccg ctcataaaac cattaccgga aaagggattg gcttccatgg
     9901 cattctgttt ttagccgcag caactaccgc tttttttcag gccattcatc aggaatccaa
     9961 acccgttatg gtcgagggta acctcggcaa gggtttcagc taatgaatct tcaatgtaga
    10021 aatctacacc atcgatgcta gtggcagttt cgccgggctg tggtgcgctt gcaacatcca
    10081 tttgcactga agggccacag catccctgcg tgctgtatat acggattccc tttgagtttt
    10141 catcaatggt ttctaaaacc tttaatagtt cttcctttgc ttttactgat agtttcatag
    10201 ttaaattatt tatggattaa atctcacaat ttactgatgc gctccttact ttaccgaaaa
    10261 aggagtcaaa ctcatcgagg tatttatgta tgctctccga gcagaggcag tagtttatcc
    10321 gtagtccatc aatttcgcca tgtatgagcc ctagttcctt tagctccttt aaatgctgcg
    10381 aaacggtagt gcggctaagc ggcaggttct gcgaaatatc gcccgatatg catgtcctgg
    10441 tttcggcaag gtactggagt attgccaacc gagctggatg cgaaatggcc ctggcaaacc
    10501 ttgccaattc ctgcagcttc tcgtcaaaaa cttctgactt attcatacca ttgtatttat
    10561 gtcgtaaaca tacgacatat ttttggctta aacaacaatt caggtaaaat gtttcaaaca
    10621 aattttaaga tattgattag tatatataca gttcttccct cgctcaaaat ctgcgatttt
    10681 tttaatccat aagtggcgga ctctgcccac atactttaca atcggtaagt tttccgttta
    10741 gggtatatgt aaa
//
LOCUS       DAOOWJ010000089        10679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6179_length_10679_cov_7.770990, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000089 DAOOWJ010000000
VERSION     DAOOWJ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6179_length_10679_cov_7.770990"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..122)
                     /locus_tag="PLW22_08850"
     tRNA            complement(49..122)
                     /locus_tag="PLW22_08850"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:Met,seq:cat)
     gene            complement(407..1324)
                     /locus_tag="PLW22_08855"
     CDS             complement(407..1324)
                     /locus_tag="PLW22_08855"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOU74852.1"
                     /translation="MEKRVSFLLLVMLAISVIGCGTKKSNEYANLESISSNRSEAFTE
                     TVVDDIAAPVAKSVAAVDIEPKVIKTANVSIEVADYLKSRQKVDSIVAKHKGFVTNEA
                     FQETDTQKSNSITIRVPAQNFDHLLSDIATIAKRVDYQNIYTQDVTEEYFDVKTRLEN
                     KQEVERTYRKHLREAKNIEDILKIENKLAEIRSEIESAQGRLKYIDNQVNLSTVSLYL
                     YQKLEYKYIPEQLPGFWQRVKEGFHWGWKGFLWFLILLVKLWPLWLVGAIAYFTVILI
                     EKQIKSRKKKEKKAKKLQKKAKQTDETVM"
     gene            complement(1330..1545)
                     /locus_tag="PLW22_08860"
     CDS             complement(1330..1545)
                     /locus_tag="PLW22_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOU74853.1"
                     /translation="MIEFDIETEYIQLDKLLKACRLVYSGGEAHQLVDEGLVKLNGTV
                     ETRRRAKIRPGDKVQVGSETIVVKTKK"
     gene            1633..2556
                     /gene="queG"
                     /locus_tag="PLW22_08865"
     CDS             1633..2556
                     /gene="queG"
                     /locus_tag="PLW22_08865"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOU74854.1"
                     /translation="MVKAEDIISIALGVGFHACGVSRAENLDNLEGVYFKWLHNQYHA
                     TMAYMERNIDLRLDPTMLVPGAKSVISVLLSYNTGDVPVNIQSPRIARYACRVDYHFL
                     MKKMLWELLNRMRKSYGEINGRAFVDSAPVMEREWAVRAGLGWIGKNSNLINRNLGSY
                     VFIGELIVDIEIEPTIHLERDGCGSCTRCMDACPNSAIVAPGTVDARKCISYLTIERK
                     QLIADDEVSNLKGWCFGCDICQEVCPWNSKAVRVNGVDQETHIFSGIKVAELSSLSES
                     EFTYKFGNTPLERAGYEKIMGAIKLITRAKG"
     gene            complement(2592..4010)
                     /gene="ahcY"
                     /locus_tag="PLW22_08870"
     CDS             complement(2592..4010)
                     /gene="ahcY"
                     /locus_tag="PLW22_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOU74855.1"
                     /translation="MEQVIQATLPYKVKDIALAEWGRKEITIAEKEMPGLMSLRKKYG
                     PHKPLKGARITGSLHMTIQTAVLIETLVELGAQVRWASCNIFSTQDHAAAAIAAAGIP
                     VFAWKGETLEEYWWCTAQALSFPEGKGPNLIVDDGGDATLLVHWGYKAENDKSFLDRV
                     PSSHEEKVILSLLKDILAKEPNKWHNLVKELKGVSEETTTGVHRLYQMLERKELLIPA
                     INVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVCGYGDVGKGCAHSMRSY
                     GARVIVTEIDPICALQAAMEGFEVKTVEDTLDEGNIYVTATGNCDVITLEHMQRMKDQ
                     AIVCNIGHFDNEIQMDRLNSQKDVKRINIKPQVDQYTFPDGHSIFILAEGRLVNLGCA
                     TGHPSFVMSNSFSNQTLAQIELWTKNLPVDVYRLPKHLDEEVARLHLEQIGVKLTQLS
                     PKQAEYIGVKPEGPYKPEHYRY"
     gene            complement(4022..5032)
                     /locus_tag="PLW22_08875"
     CDS             complement(4022..5032)
                     /locus_tag="PLW22_08875"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOU74856.1"
                     /translation="MSKHKPNMIIIQRPNQTRRKELALTLGFFDGVHLGHRELIQTVV
                     HDAEIMGLESAVLTFWPHPRLVLHKDPEKLRFLNTLNEKAKIIAKFGIDYFIVQEFSR
                     DFFNLDATDFIKLLVYEYKVKHFVIGADHRFGKGGSGDSDLLNKLSGEMGFTVKVIPS
                     QRIEGIDVSSTKIREALLTGNLELANQMLGYPYLITGAVQSGNQLGRRIGFPTANIRP
                     NDPLKLIPKEGVYAVVVNANDTIYKGMLNIGFRPTVGTDNKQTIEVNIFDFEEDLYNS
                     GIEVALVARLRDEKRFASVELLKEQLVIDKQNALEALKNEKVERYQNLFLTLRPERNN
                     NQ"
     gene            complement(5188..9441)
                     /gene="glgP"
                     /locus_tag="PLW22_08880"
     CDS             complement(5188..9441)
                     /gene="glgP"
                     /locus_tag="PLW22_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOU74857.1"
                     /translation="MKDKLLKPDYLFEVSWEVCNKVGGIHTVVSTKASLLEDELKNNY
                     ITIGPDIIKDTERNFEFTEDPNLFKAWKSQALSEGISVRVGRWNIPCKPIAILVNFSG
                     LISQKDTIFKLLWERFKLDSITGQWDYIEPALFGYAAGKVIESFTNFNLSLRHRVVAQ
                     FHEWMTGAGLLYLKQNSPQIGTVFTTHATVLGRSIAGNGIPLYSNIDKYNPDHKAKEF
                     NVVAKHSLESIAAKEADCFTTVSEITAQECKYFLGNEVDLITPNGFDGSFIPKPEEEF
                     KLRKLEGKIKFYEVAEAMLSNDVAKDSLVIGISGRYEFKNKGIDVFLDALAKLNRENN
                     LEKEVLAFLLIPAGHHGPRKDVFHNLRDGKESDNYIKLDDTHVTHYLNDPESEIILKR
                     IRALGLNNASTDKVKVFWVPCYLNGNDGIFNKPYYDLLIGMDITVFPSYYEPWGYTPL
                     ESLAFRVPTITTTLAGFGDWINKHYNNPKDAIRIIHRTDDNDPEVVEQIALTIDYIMK
                     LDNNKKLNLAHNADEISRVALWENFIDYYFQAYDKALKVVGDRTQMYMEFERQEVLPT
                     VERKFKQYQPNWVRLIIQKRLPEKLKPLDEISRNLWWSWNTDAKEIFETIDKDLWNEC
                     EHNPIQFLERISLPRFQELENDTAFVDKLQSVYLKFTSYMMKRYERKGPRIAYFSMEF
                     GLHSSLKLYSGGLGVLAGDYLKEASDRNIDMVGIGLLYRYGYFTQNLSASGQQVAVYD
                     QQNFTQSVATPVRNENGKWITVKVAFPGRDVFVRVWKVEVGRTDLYLLDTDFEDNQPL
                     DRTITHHLYGGDLENRFKQEMILGIAGIRTLNELGITPDIYHINEGHAAFIGLERLRR
                     YIVEEKLSFPEALELVRASSLFTTHTPVPAGHDAFPEELVRTYMAHYPERLKITWDQF
                     MDLGRMTPGDTTERFSTSHLAIRLSQEVNGVSWLHGEVSRKMFAGMWPCYFPNELHIS
                     YVTNGVHFPTWTAREWKAILEDSSDSSELGYTLPNWNAIENLPDNKIWDIRNKLRKRL
                     IKLINKRLNNPNLVRFESPRQVIEIQEKLNDKILTLGFARRFATYKRAWLLFKDQDRL
                     AELVNNPQRPVQIFFAGKAHPHDKAGQDLIKKIIEISKQPRFLGRIIFLQNYDMELAR
                     RMVQGVDVWLNTPTRPMEASGTSGQKAVMNGVLHFSVLDGWWVEGYRENAGWALPMEP
                     TYPQEEFQDELDAELIYTILENEVVPAFYERTADDVPQKWISFIKKSMTQVASNFTTL
                     RMINDYQNRFYNKLYYRFNELSENDFEEAKQISAWKRRITRSWDEIEVIRVKQFDMNR
                     EPILLGNEYEAEVVLDLGSLAPDEIGVELVVADLIENQQVTVKRVHEFELAGIEGSRA
                     TYRLKLIPVEPGAFECGIRIFPKNPLLPHRMDFCLVRWI"
     gene            complement(9903..10679)
                     /locus_tag="PLW22_08885"
                     /pseudo
     CDS             complement(9903..10679)
                     /locus_tag="PLW22_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
BASE COUNT         2714 a         2282 c         2387 g         3296 t
ORIGIN      
        1 aaaataaaaa agcccgcaaa ctcactgttt acgggctttt cgactttgta gcgagagccg
       61 ggattgaacc ggcgacctca tgattatgaa tcatgcgctc taaccaactg agctacctcg
      121 ccaactaact tatatttcaa aaaagaacgt ttacatttcg aaccggcgac cttccccata
      181 aaatcgggac gctctaacca actgagctac ctcgccatca ctattttaaa ttgccacttg
      241 cttcgatatg cagcattcta gcatgtttca gcaggtaatg caattttttt tagcactgca
      301 aaaataagta aattttttat tcgcaaaata gttttaaggt tatttgcaat tttttctgca
      361 gggcagtcta attcaacgct gtatttcaag ccaaaagggt tacccgttac atgacggttt
      421 catcggtttg cttggccttt ttttgcagtt tcttagcctt cttttccttc tttttgcgac
      481 tttttatctg cttttcaatt agaataactg taaaatatgc aatagcacca actagccaga
      541 gtggccaaag cttaaccaga aggattagga accaaagaaa acctttccat ccccaatgga
      601 atccttcttt taccctttgc cagaacccag gcaattgctc agggatatat ttgtattcca
      661 atttctggta cagatataac gacacggtgc ttaggttaac ctgattgtcg atatacttta
      721 accttccctg agccgattca atttcggaac ggatttcggc caacttgttc tcaatcttca
      781 gaatatcctc aatgttcttt gcctcgcgca gatgtttcct gtaggttctt tcaacttcct
      841 gcttgttctc caagcgtgtt ttaacatcga aatactcttc ggttacatcc tgggtatata
      901 tattctggta atcgactctt ttggcaatag tagcaatatc gctaagtaga tggtcaaagt
      961 tttgagccgg taccctaatg gttatgctgt tacttttttg ggtatcggtt tcttggaatg
     1021 cttcgttagt tacaaagcct ttatgcttgg ccacaattga atctactttt tggcgcgatt
     1081 ttaggtaatc ggctacctca atgctaacat tggctgtttt aattactttt ggctcaatat
     1141 caaccgcagc aacacttttt gctacaggag ctgcaatatc atcaacaacc gtttcggtaa
     1201 aggcttcact ccgatttgaa ctaatcgatt cgagatttgc gtactcattt gacttttttg
     1261 tgccacagcc aataactgat attgccagca ttaccagcaa aagaaaagat actctttttt
     1321 ccataacatc tatttttttg tttttaccac tattgtttca gaaccaacct gaaccttgtc
     1381 gccaggtctt atttttgctc tccgccgggt ttcaactgta ccgttcagtt taacaagacc
     1441 ttcgtcaaca agctggtggg cttcgccacc gctataaaca aggcggcacg ccttgagtag
     1501 cttatccagc tgaatatatt ccgtttcaat atcaaactca atcatactaa tatgtcaaat
     1561 ttttaccaag ttaagtaaaa aagctaaatt tcgattctat ttaacaatct ttgtatttgc
     1621 aaatacttac cgatggtaaa ggctgaagat attatcagta ttgcgctagg tgttggtttt
     1681 catgcttgtg gggtgagtcg ggccgaaaac ttggataatc ttgaaggggt atacttcaag
     1741 tggttgcata atcagtacca tgcaaccatg gcctacatgg agcggaatat tgaccttagg
     1801 cttgatccta ccatgttggt tccaggggcg aaatcggtga taagtgtttt actttcgtac
     1861 aatacaggcg atgtgcctgt taacatacaa tccccccgaa ttgctcgtta tgcctgcagg
     1921 gttgattacc attttctgat gaaaaaaatg ctctgggagt tgttgaacag aatgcggaaa
     1981 agctatggtg aaataaatgg acgagcattt gttgattctg cgccggtaat ggagcgtgag
     2041 tgggcagttc gtgctgggtt gggatggatt gggaagaact cgaaccttat taacaggaac
     2101 ttaggttcct acgtttttat tggggaactt attgttgata ttgaaattga gccaaccatt
     2161 cacctggagc gagatgggtg tggctcttgt acccgatgca tggacgcctg ccccaatagt
     2221 gctatagttg ccccgggtac tgtagatgcg cggaagtgca tatcgtactt aactattgag
     2281 cgtaaacagt tgattgccga tgatgaggtg agcaacctaa aaggatggtg ttttggctgc
     2341 gacatctgtc aggaggtttg cccatggaac agcaaggctg tgcgggttaa tggagttgat
     2401 caggaaaccc atatttttag tgggattaaa gttgccgaac tttcaagtct tagcgagagt
     2461 gaatttactt ataaatttgg caatactccg cttgaacgcg caggatatga aaagattatg
     2521 ggagcaataa agttaattac tagagcaaaa ggatagaaaa actgccggag gttcccggca
     2581 gttctaattg tttagtatcg gtaatgttcg ggtttgtatg gtccttcggg tttaacacca
     2641 atatattccg cttgttttgg gctcaactgg gttagtttaa caccaatttg ctcaaggtgc
     2701 aagcgagcaa cttcctcgtc gaggtgtttg ggaagtcggt acacatcaac aggtaggttt
     2761 ttagtccaga gctcaatttg tgcaagggtt tggttggaga aactgttgct catcacaaag
     2821 ctgggatggc ctgtggcgca gccaaggtta accagtcggc cttcggccag gataaaaatg
     2881 gagtggccat cggggaatgt gtactgatcc acctgtggct taatattgat gcgcttaaca
     2941 tccttttggg agttgagcct atccatttga atctcgttgt caaaatggcc gatattgcaa
     3001 acaatggctt gatccttcat gcgttgcata tgctcaagag taataacatc gcagttgcct
     3061 gtagcggtaa cgtagatatt accctcatcg agagtgtcct caactgtttt cacctcaaaa
     3121 ccttccatgg cagcctgaag ggcgcatatg ggatcaattt cagttacaat cacacgggcg
     3181 ccgtaggagc gcatgctgtg agcacaaccc tttcctacat cgccgtaacc gcataccaca
     3241 acaaccttac cggcaatcat aacatcggtt gcccgcttaa tgccatcggc aagcgattcg
     3301 cggcacccat agaggttgtc gaacttactc ttggtaaccg agtcgttaac atttatggcg
     3361 ggtataagta gttctttacg ctcaagcatt tggtaaaggc gatgaacccc ggtagtagtt
     3421 tcttccgaaa cacctttaag ttccttcacc aggttatgcc acttgttagg ctctttagcc
     3481 aaaatatctt tgagtagtga gagtataacc ttttcttcgt gcgacgatgg caccctatcg
     3541 aggaacgact tgtcgttctc ggctttgtaa ccccaatgta caaggagtgt agcatcgcca
     3601 ccatcgtcaa caatgaggtt tgggccttta ccttcgggga acgaaagagc ctgcgcggta
     3661 caccaccagt actcttcaag tgtttctcct ttccatgcaa atacaggtat gcctgcagct
     3721 gctatggctg ctgcagcatg atcctgagtt gagaaaatgt tgcaacttgc ccaacgaacc
     3781 tgtgctccca gttctacaag ggtttcaata agtacagcgg tttgtatggt catgtggagc
     3841 gaaccggtaa tgcgtgcccc tttaaggggc ttgtgaggac cgtacttctt acgaagcgac
     3901 attagtcccg gcatttcctt ttcggcaata gtgatttctt tccttcccca ttctgccaat
     3961 gcgatatcct taaccttgta gggtaaggta gcctgaatta cttgttccat taatattgat
     4021 tttattggtt attattcctt tcgggccgta aagttaggaa taagttttga taacgttcaa
     4081 ctttttcgtt ctttaaggct tccagggcat tctgtttatc aataacaagt tgctctttca
     4141 agagttcaac ggaggcaaaa cgtttttcgt cccttaaccg ggcgacaagg gcaacctcaa
     4201 tgcctgaatt gtaaaggtct tcctcaaaat cgaaaatgtt tacttcaata gtttgcttgt
     4261 tgtcagtacc aactgttggt ctaaaaccaa tgttaagcat tcccttgtaa atggtgtcgt
     4321 tggcgttaac aaccactgca tatacccctt ccttgggaat aagcttaaga gggtcgtttg
     4381 gacgaatgtt ggcagtgggg aaaccgattc ttcgtccaag ctgattaccg gattgaacag
     4441 caccggttat aaggtatggg taacccagca tctggtttgc cagttcaagg tttccagtta
     4501 ggagcgcttc gcgtatcttt gtggagctaa catcaattcc ttcaattctt tgcgatggga
     4561 tcactttcac ggtgaaaccc atttcgccag aaagtttatt caaaaggtct gaatcgcctg
     4621 atccaccttt tccaaagcga tgatcggcac caattacaaa atgcttaacc ttatattcgt
     4681 aaaccagcaa tttgatgaaa tcggtggcat ccaaattgaa aaaatcccgg gaaaattcct
     4741 gaacaataaa atagtctatt ccaaacttgg caattatttt agctttctcg ttaagcgtgt
     4801 taaggaaacg aagtttctca ggatctttat ggaggacaag tctggggtgt ggccagaaag
     4861 ttagcacagc cgattccaaa cccatgattt cggcatcgtg aactactgtt tggattagct
     4921 cgcggtgtcc aaggtgaacg ccatcaaaaa agcctaaggt aagtgctaat tccttacggc
     4981 gcgtttgatt tggacgttga ataataatca tgttaggctt gtgcttactc acatgctaat
     5041 ttgcaaaaat ttattgtttc aaagtaatat tattgttcat gatatttttc ttgttgacca
     5101 aagcataaaa agcattgacg aaccgacgta aacttttggt aaggggaaaa ggcttgcatt
     5161 aaccttttcc ccaatgcggt ttttccatta tatccatcga accaagcaaa aatccatgcg
     5221 gtggggcagt agtgggtttt ttgggaaaat tctaatacca cactcaaacg caccgggttc
     5281 aacggggatc agttttaacc tgtaagtagc tcttgagcct tcaattccag caagttcaaa
     5341 ttcatgaaca cgcttaacgg ttacttgttg gttctcaata aggtcggcaa ccacaagctc
     5401 tacccctatt tcatcggggg caagtgaacc caaatcgaga acaacttcag cctcatactc
     5461 attacccagc aatatgggtt cacggttcat gtcaaactgt ttaacccgta tcacttcaat
     5521 ctcgtcccag ctacgagtaa tgcggcgttt ccatgcggaa atctgtttgg cttcctcaaa
     5581 atcgttttcg gatagctcat taaacctata gtatagcttg ttgtaaaacc ggttttggta
     5641 gtcgtttatc attctaagcg ttgtaaagtt cgatgccact tgggtcatgg actttttgat
     5701 aaacgaaatc cacttttgag ggacatcgtc ggcggtgcgc tcatagaatg cgggtacaac
     5761 ctcattctca agtatggtat agatgagttc agcatcgagt tcgtcctgga actcctcttg
     5821 ggggtatgtg ggttccattg gtagtgccca accggcattc tcgcggtagc cttcaaccca
     5881 ccatccatcc agaacgctaa agtgaagcac tccattcatt acggcttttt gaccgctggt
     5941 tcctgaagct tccatgggac gggtgggggt gttgagccaa acatcaacac cttgtaccat
     6001 tcgtcgggcc agttccatat cgtagttttg caggaagatt attctgccaa ggaaacgtgg
     6061 ttgcttggat atttcaataa tcttcttgat taaatcctgg ccggctttat cgtgtgggtg
     6121 ggctttgcca gcaaaaaaga tttgaacagg tcgttggggg ttgttgacta gttccgctaa
     6181 cctatcctga tccttaaata gcaaccatgc ccttttgtag gtggcaaatc ggcgggcaaa
     6241 acctagggta agtatcttgt cgttcagctt ttcttgtatc tcaataacct gacgtgggct
     6301 ttcaaaccta accaggttcg gattatttaa ccgcttgtta attagtttga taaggcgttt
     6361 ccttagcttg tttcgaatat cccatatctt attatcgggc aagttctcaa ttgcgttcca
     6421 gttaggaagt gtgtagccca attcagaact gtcagaacta tcttccagta ttgctttcca
     6481 ttcacgggca gtccatgtag gaaagtgaac gccatttgtt acatagctta tatgaagttc
     6541 attggggaag tagcagggcc acatgccggc aaacatttta cggctaacct cgccatggag
     6601 ccagctaacc ccgttaacct cttgcgagag ccttatggct agatggctcg ttgaaaaacg
     6661 ctcggttgtg tcgccagggg tcattcggcc taaatccatg aattgatccc aggttatttt
     6721 taacctttct gggtaatgag ccatgtaggt tcgtactaac tcttcaggga aggcatcgtg
     6781 gccggcagga acaggtgtat gggtagtgaa aaggctcgag gccctaacaa gttccaaagc
     6841 ttcagggaat gatagttttt cttctacaat gtaacggcgt aaacgttcta gcccaataaa
     6901 tgcggcatga ccctcgttga tgtggtagat atcgggggta attcctaatt cgtttaaggt
     6961 tctaatgccc gctataccaa gaatcatttc ctgcttaaac cggttttcca agtcgccacc
     7021 gtaaaggtgg tgagtgatgg tacggtcaag gggctggtta tcctcaaagt cggtgtccag
     7081 gaggtaaagg tcggttctcc ccacttcaac tttccaaacc cggacgaata catcgcgtcc
     7141 agggaatgcc accttaacgg tgatccactt accgttttcg ttacgcacag gggtggcaac
     7201 gctctgggta aagttttgct ggtcgtaaac agcaacctgt tggccggaag cggaaaggtt
     7261 ttgtgtgaaa tatccatacc ggtataaaag gcctatacca accatatcga tattgcggtc
     7321 cgaagcctct ttcaggtagt cgcctgctag tacaccaaga cctcctgagt aaagtttaag
     7381 tgagctgtgc aggccaaact ccatgctgaa gtatgcaatt cgggggcctt ttcgctcgta
     7441 gcgtttcatc atgtatgagg taaacttcag gtaaaccgat tgaagtttat cgacaaacgc
     7501 agtatcgttt tctaattcct ggaagcgggg taaacttatt ctttccagga actgaatggg
     7561 gttgtgctcg cattcgttcc acaagtcctt atcaattgtt tcaaatattt ctttggcatc
     7621 ggtattccag gaccaccata ggttacgtga aatttcatcg aggggtttta gtttttccgg
     7681 taagcgtttt tggattatca acctaaccca gttgggttga tactgtttga atttccgctc
     7741 aactgtaggc aaaacctctt gccgttcaaa ctccatgtac atttgtgtgc gatcgcctac
     7801 aaccttaagg gctttgtcgt atgcttgaaa gtagtagtca ataaagtttt cccaaagggc
     7861 aacgcgcgat atctcgtcgg cattatgggc tagattcaac ttcttgttgt tgtcgagttt
     7921 catgatgtag tcgatggtta aagcaatttg ctcaaccact tcggggtcat tatcgtcggt
     7981 acggtgaatt attctaatgg catccttggg gttattgtaa tgcttattga tccaatcgcc
     8041 aaaacccgca agggttgtgg taattgtagg aactctaaag gctaaacttt caagcggtgt
     8101 ataaccccag ggttcatagt acgatgggaa aacagtgata tccatcccta tgagcaaatc
     8161 gtagtatggc ttattaaaaa taccatcgtt tccgttgagg taacagggga cccagaatac
     8221 tttaacctta tcggtggaag cattgtttag tcccagtgca cgaatccgtt ttagtattat
     8281 ctcggattcg ggatcgttga ggtagtgtgt aacgtgcgta tcatcaagtt tgatgtagtt
     8341 atcgctttcc ttcccatcgc gtagattatg gaatacatct ttcctgggtc cgtgatgacc
     8401 tgcaggaatc aacaggaatg caagtacctc cttttcaagg ttattttcgc ggtttagctt
     8461 tgcaagcgca tccaggaaaa catcaattcc tttattcttg aactcgtaac gcccgctgat
     8521 gccaatcact aagctatcct ttgctacatc gtttgagagc atagcctcgg caacttcgta
     8581 aaactttatt ttgccttcaa gtttccttag cttaaactcc tcttcgggtt tagggataaa
     8641 ggagccatcg aaaccgttag gagtaataag atcaacctca ttgccaagaa aatacttgca
     8701 ttcctgagct gtaatttcgc ttacagtggt aaaacaatcg gcttccttgg cagcaattga
     8761 ttcaagggag tgcttggcta caacgttaaa ttccttggct ttatgatcgg gattatactt
     8821 atcgatattg ctgtatagtg ggatgccatt accggcaata ctacgtccta aaacagtggc
     8881 atgggtggtg aatactgttc ctatttgagg tgaattttgc tttaggtaaa ggagtccagc
     8941 gcctgtcatc cattcatgga attgggcaac caccctgtgg cgtaggctca ggttgaagtt
     9001 tgtgaaactc tcaattactt ttccggcagc ataaccaaaa agggctggct caatgtagtc
     9061 ccattgtccg gtaatggaat cgagtttaaa acgttcccaa agcagcttga atatcgtatc
     9121 tttttgtgat attaagcctg aaaagtttac cagtatagca atcggcttgc agggtatatt
     9181 ccatcgcccg actctcactg agataccttc cgataaagct tgacttttcc atgctttaaa
     9241 aaggttggga tcttcagtaa actcaaagtt gcgttctgta tctttgatga tatcggggcc
     9301 aatggtgatg tagttgtttt taagttcatc ctccagcaac gaagccttag tggaaacaac
     9361 ggtatgtatt ccacctacct tgttacaaac ttcccagctt acctcgaata ggtagtcggg
     9421 cttcagtaat ttatccttca tactttgaac ctttatttag ttttgttttt cccggtagat
     9481 tttcggggtg tagtgctttc ctttttctcc ttaacggctt tgctcttagt tgctgatgtt
     9541 ttagctttag cagttgttgt tttagctttg gcagttgctg ctttagcttt ggatgccgat
     9601 gcttcagttt tcttgaccgt agtcttcgga acttcagttt tacctaccgg cttggctttc
     9661 atggattttg gggtttctgt tttgacagtc tttgaggtag ctttttttac cgattcctgg
     9721 gtggttgaag ttttggtagg cttaggcttt gctgattttg ttgtttttgg cttaacttct
     9781 ttagctttca gcacggcctg aagttcatta atttttccct gatataattc aatgagtttg
     9841 atagcctctt cgggattgtc aatcttttcg gtttcagctg gttcttcaat cagcttgctt
     9901 ttaagcctta tgatgaaatc gctaagaaca ttcataaagt ttatgaatgc ctcgtatgga
     9961 gtgtcgtatg ggttaaagta tttatgtacg tcgccatcgc tgaaccattt ggtgcacatg
    10021 tagtagaaat ggtcgctggt ttgcaggtaa agccagtcgc gtttgatgtt ggggtcgtca
    10081 attttttcaa ccatatcggc taggttgtaa agctcattga aagcgctatc ctgcagttca
    10141 ttaccaagcc atgccgtaag gtcacgttcc tcatcggccc aggagatggg gtgtggaacg
    10201 tgaagcagag ccacgggttg atgcttctgt gaagcctccg agggggttag aaattcaaag
    10261 ttggtattgg caaaaacctt accgggtaaa gcacgcataa agtcgaaaat accagtttcc
    10321 gcccactgat gttcaccaaa agtttcgtaa tccatgaaaa ggtttacaat ttcctcattg
    10381 gggttgatgt tgttcaacca gtgcacatac ttttcggtgg taagaggcca ctcgctccag
    10441 tcgcggttgg agaacctaaa ggcaatatca tcacttaacc taaagttctt tagtagtaat
    10501 cgaagctttg ggttaatggc attggagtaa acaaagttag agctcttcca gccaaggatg
    10561 tgtttggctc cctcggtgag catggtattc aaacccatgt cggcaaccat tgcgcctatc
    10621 tcatcggaat atatgagctc tgtattgcgg aagcttttgg gcttataccc tataagatt
//
LOCUS       DAOOWJ010000090        10679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6180_length_10679_cov_7.542545, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000090 DAOOWJ010000000
VERSION     DAOOWJ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6180_length_10679_cov_7.542545"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2691
                     /gene="ppdK"
                     /locus_tag="PLW22_08890"
     CDS             <1..2691
                     /gene="ppdK"
                     /locus_tag="PLW22_08890"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOU74858.1"
                     /translation="KTAEGKADMKNLLGGKGANLAEMCLLGLPVPAGFTITTDTCNEY
                     YKNNHELPAELIPEIWDAMAKVEQMMGMKYGDAENPLLVSCRSGARKSMPGMMDTVLN
                     IGLCSSTIPGLIKKTNNPRFVWDSYRRLIMMYADVVMEKAEGIEPVDGKGIRKQLDDI
                     LDEYKHSKGYKSDTDLTADDLKFLAEEFKKQVKKVLGKEFPDDAKEQLLGGIKAVFKS
                     WNGKKAVSYRRIEGIPDDWGTAVNVQTMVFGNMGDTSATGVAFTRNPATGDNVFYGEW
                     LINAQGEDVVAGIRTPNPLNNDTKNEQNKHLPSMEELMPGTYKELVEIRNTLEKAFKD
                     MQDIEFTIQEGKLYMLQCRAGKRTGTAALNMAMDMLHEGLIDEKTAVLRVDPIQLDEL
                     LHPICDPKEEAKVTPLVKGLPAGPGAAVGKVVFTAEDAVAWARKGEKVILLREETNPE
                     DVEGMRAAMGILTARGGMTSHAALVARGWGKCCIVGAGALKVNAADKTAKIEGSSVVI
                     KEGDILTLNGTKGHVYNAELKLMEATENPRFQEFMKLVDKYRKMGVRTNADTPEDAKV
                     ARDFGAEGIGLFRTEHMFYGKGSEEPLFLLRKMILSSTEAERRKALDELFVYVKRDVK
                     NTMLAMDTLPVTFRLLDPPLHEFVPQSPEKQAELAKALGITVEAIVKRGEALHEVNPM
                     MGHRGVRLGVTYPEVSEMQIRAIFEATAELMKEGKTPKPEIMVPVTCDVSELDFTKKI
                     VDRVYAEVCNKFGIKAIEYKYGTMIEIPRACLLADRMAKTAEFFSFGTNDLTQMTFGF
                     SRDDIGGFLHDYLDKKMLAADPFQTIDQDGVGQLIEMAVAKGRNTRPELKVGICGEQG
                     GDPASVEFCFKNGLTYVSCSPFRVPIARLAAAQASIKYGK"
     gene            2769..4172
                     /locus_tag="PLW22_08895"
     CDS             2769..4172
                     /locus_tag="PLW22_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOU74859.1"
                     /translation="MYLKLRYYLFIPLLLFIGKTNITFGQDPVIDSAWVISTYNYLLM
                     VEADSGNIEKVRTLLYLGADPNTSDENGVTPLMFAVQSGNIDLVELLIDNGANVNAYP
                     TNGNTALHAAVIAANDSVAELLIKKGADVNAKNVKGLTPLHFAVWYGLPYLTDILIYY
                     GSDLNAIDYQGNTPLMLAVFSGAKLSTRLLLENGANPNIPDNKGVTPLMVAAQFNDTV
                     ICNYLTTYGADLSLCDSRGANALCYAIANKSNEIVKCLSEFDALKFPLKKSYYQIARE
                     YNNKEAMQFFASNGIKTKIKPSITSVYFGIGTSFSKHEFLLGYHLGVFEQITQTEFSL
                     GYLYRPSATASLTTIDGQLYQYWEKRNILQLNIRKYYNLFSYKGKNIISFAAIGAGPA
                     FRNYRGTSSNPKTHFTTNFSGGLSIKGDAMEYSIGVGYNFYKELGVSPYFYTISAKIH
                     FNVLNPKVVYKNVKHVQ"
     gene            4162..4584
                     /locus_tag="PLW22_08900"
     CDS             4162..4584
                     /locus_tag="PLW22_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74860.1"
                     /translation="MFSKIFKLLTILLIVLAPYSCEEMEFFIVNCDECYELKPDKIDV
                     RLKFTINNENQVVYFELYSGNPENGVLFFSGNTTISEKVFQFDANSYYSIKAYYTSKG
                     RQIIVIDATTVKLKYDKSSCSSPCYTISGDILDARLRY"
     gene            4722..6419
                     /locus_tag="PLW22_08905"
     CDS             4722..6419
                     /locus_tag="PLW22_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOU74861.1"
                     /translation="MKRQLLIRDVTLRDGQQSLFATRMTQKQVERVLPYYREAGFYAM
                     EVWGGAVPDSVMRYLNEDPWYRLESIKAAIGDTSKLTALSRGRNLFGYNPYPEEVIEG
                     FNRNAVKSGISIMRIFDALNDTENIRSTVKYVKENGGMADCTVCFTVDPKFSRKDRIK
                     AMLSGKTLPRKIFTEKYYIAKAKELEAMGADMITLKDMAGLVTPEQSAAIISALKREI
                     KVPIDFHTHCTPGYGLASTLTAIVNGVDIVDTAIWNFAGGPAAPAFELIQIFCDKMGI
                     DTGVNLKAVASINAELKTIRTELKELDTYELPIDFDITSYNLPARIDNLFEQAILFAQ
                     SGKYDALLDACQAIERYFNFPEPDENVKNAEIPGGMYTNMLAQLKQLKLENLLPRVLE
                     IIPTVRLDAGCPPLVTPTSQIVGAQAVNCAIDESKGQPFYTNKSIQFVNLVKGIYGRT
                     PLPIDPQFRLKIAGVAEETPYDTSNYKKQPNPAFPEYGENVKLAMNEKEELLLELFPT
                     VAEKYLRDKVVNHYESIKRQKEEEVQRKIQEEKQKYYSMTEEQRWKRLLDGLQQYFC"
     gene            complement(6476..8977)
                     /locus_tag="PLW22_08910"
     CDS             complement(6476..8977)
                     /locus_tag="PLW22_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOU74862.1"
                     /translation="MKKLFLLLLAVMANVLLVISQPVHVTKANYDLPARFSPKKIDKM
                     VFSTSVDAHWLKKSTRFWYSYETPSGKSYYIVDPAKKSKSLLFDNVDMAAQLSRLTKE
                     PFDAQHLPIQKIKFIKNETVIQFEVKSTLVDEDKTDDEENDMKQKKDDKAKAKKDKKT
                     YYFEYDLNTRKLTLLDDYKKPKENPEWANISPNGEYVVYSKNHNLFWMDKENYEKAKK
                     NEKDSTIVEHQLTTDGEKYYSYGYNTKEEDNVEEVKNKDNRKPAYITWSHDSRYFAMV
                     RTDQRKVKDLWVIKTLSNPRPTLETYKYHMPGEKEAPQDEILVFDMNNRDKVIKPDIK
                     AFKDQDVSILNYRRLASERDDDYVPTRWLSKYNDRLYFQRTSRDLKRIDVCYVDIKTG
                     EVKTVIEERLNTYIDFNGIALINNEKEIIHWSERDGWGHFYLYDNQGNLIRQITSGPF
                     HCERLVGYNETTKTLFFTANGREPNEDPYYLHLYRIGIDGTGLRLLNKGNFDHSISMD
                     DNQQYIVNNYSRVNTIPESKLIDNQGNDLLFLEKADLTLLFEAGYKFPEPFTVKAADG
                     ITDLYGVMYKPFDFDSTKQYPIILYVYPGPQTEAVNKSFSARMDRTDRLAQFGFIVIT
                     VGNRGGHPARSKWYHNYGYGNLRNYGLADKKAATEQLAYRHKYIDINRVGIHGHSGGG
                     FMTAAAMLTFPDFFKVGVANAGNHENNIYNRWWSEKHHGVKEIADTAGNVTFEYNIDK
                     NSEIAKNLKGHLLITTGEIDNNVHPGNTIRLANALIKANKRFDFFMYPGQRHGYGDMT
                     EYHFWLTGDYFCKHLLGDYDTSIDITPMNNEIERK"
     gene            9077..>10679
                     /locus_tag="PLW22_08915"
     CDS             9077..>10679
                     /locus_tag="PLW22_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU74863.1"
                     /translation="MKKLFILLLCIYPLLGNAQYFPVTIAEGSNFQSTSTYADVMNYI
                     KMLEKASKHIKTETIATSIEGREIPLMVVANPMPKTPRDIGNRIVIYIQANIHAGEVE
                     GKEASLMFVRDLLKNPNNPLFKNIVLLVCPILNADGNESISPKNRLWQNGPVNGVGVR
                     HNGQMLDLNRDAMKLETPEMVGVVTNVLNRWDPSIVMDCHTTNGSYHQEPVTFTWMMN
                     PNGNRELINYMRDRMMPWVSTQLSVKYKTLNCFYGEFIDQKDYEKGWISYASEPRYFT
                     NYIGLRNRLSILNENYVYAPFDERVYGCYNLIASVCDYAMQNSKEIKDLLAKADREAI
                     QFPKTTPAPEFAITYEGRPTPQWVTILTYEAEAYTDEQGRERFRKTDRKRTVTVPYIA
                     DYYPTSTIPLPFAYIVRNDKVVTNLLKNHGIQFQTLTDTVTLNVEQFTIDSLKPAPRI
                     NQGHYNSNIFGKYVKANRLFTTNYVMVKTSQPLGQLAAYLLEPQSDDGLVYWNFWDRY
                     LVPQWGNNYNPVPVFRITDEKEFGRV"
BASE COUNT         3059 a         2410 c         2352 g         2858 t
ORIGIN      
        1 aagacggccg aaggaaaagc agacatgaag aatctgcttg gcggtaaggg tgcaaacctt
       61 gcagaaatgt gcttgttagg tttaccagta cccgcaggtt ttaccattac aaccgatacc
      121 tgtaatgagt attacaagaa taaccatgag ctacctgccg aattgatccc tgaaatatgg
      181 gatgccatgg caaaggttga gcagatgatg ggcatgaagt atggcgatgc agagaatcca
      241 cttctagtga gctgccgctc aggtgcccgc aagtccatgc ccggtatgat ggacaccgta
      301 ctgaacattg gtctttgctc ttcaacaatt cccggtctta ttaagaaaac caacaaccca
      361 agatttgttt gggactcgta ccgtcgtcta attatgatgt atgccgacgt tgtgatggaa
      421 aaggcggaag gcattgagcc agttgatggt aagggaatcc gcaagcaact cgatgatatt
      481 ctcgacgaat acaaacatag caagggatac aagagcgata cagatttaac tgccgacgat
      541 cttaaattct tagctgagga gttcaagaag caggttaaga aggttcttgg caaggaattc
      601 cccgatgatg ctaaggaaca actcctgggt ggtattaaag ccgtgttcaa gagctggaat
      661 ggtaagaaag ccgtttcgta tcgtcgtatt gagggtattc ccgatgattg gggaacagcc
      721 gttaacgttc aaaccatggt attcggtaac atgggcgata cctcagccac cggtgtagcc
      781 tttacccgca accctgcaac cggcgacaat gtattctatg gtgaatggct tatcaatgcc
      841 cagggtgagg atgtggttgc cggtattcgt acgccaaacc ctcttaacaa cgacaccaag
      901 aacgagcaaa ataagcatct tcccagcatg gaggaactta tgccaggcac ctacaaggaa
      961 ctggttgaaa tccgcaacac ccttgaaaaa gctttcaagg atatgcagga tattgagttc
     1021 accattcagg aaggtaagct atacatgtta cagtgccgtg ccggtaagcg tacaggtact
     1081 gctgccctga acatggccat ggatatgcta cacgaaggat tgatcgatga aaagaccgcc
     1141 gttcttcgtg ttgaccccat acagctcgat gaactacttc accccatttg cgatcccaag
     1201 gaagaagcta aagtaactcc gcttgtaaag ggattacccg ctggtcctgg tgcagcagta
     1261 ggtaaagttg tattcacagc cgaagatgca gtggcatggg cacgtaaagg cgaaaaggta
     1321 atcctgctcc gcgaagaaac caaccccgag gacgtagaag gtatgcgtgc cgcaatgggt
     1381 attcttaccg ctcgtggagg tatgacctca catgcagctc ttgttgctcg tggctggggt
     1441 aagtgctgca ttgttggtgc aggagcactc aaagtaaatg ctgctgataa aactgccaag
     1501 attgaaggtt caagcgttgt tattaaagaa ggtgatattc taaccctcaa cggtaccaag
     1561 ggacacgtat ataatgctga actaaaactt atggaagcca ctgagaatcc tcgcttccag
     1621 gagtttatga agttggttga caagtaccgt aagatgggtg ttcgcaccaa tgccgatacc
     1681 cctgaggatg ccaaggtagc acgtgacttt ggagccgaag gtatcggtct gttccgtacc
     1741 gagcatatgt tttacggtaa gggttccgaa gagccattgt tcttgctccg caagatgatt
     1801 cttagctcaa ccgaggcaga gcgccgtaag gcattggatg agctattcgt ttacgttaag
     1861 cgtgatgtta agaacaccat gcttgccatg gacaccctac ccgttacctt ccgtctgctc
     1921 gacccacccc tacatgagtt tgtaccccaa agccccgaaa aacaagctga actagccaag
     1981 gctttaggaa taacagttga agcaattgtt aaacgtggcg aggcccttca cgaggttaac
     2041 cccatgatgg gtcaccgtgg cgtgcgcctt ggtgtaacct atcccgaagt ttccgaaatg
     2101 cagatccgtg ccatttttga ggctacagct gagctgatga aggaaggcaa aactcctaag
     2161 cctgaaatca tggtacccgt tacctgcgat gtatcggaac tcgatttcac caagaaaatt
     2221 gttgaccgcg tttacgctga ggtttgcaat aagtttggca tcaaagccat tgagtataag
     2281 tacggcacaa tgattgagat acctcgcgcc tgcttacttg ccgacagaat ggcaaaaaca
     2341 gccgagttct tctcctttgg taccaacgac ctcacccaga tgacctttgg tttcagccgt
     2401 gatgatattg gcggattcct ccacgattat ctggataaga aaatgcttgc agctgaccca
     2461 ttccaaacca tcgatcagga tggcgtaggc cagctaattg agatggcagt ggccaagggc
     2521 cgcaatacac gccctgaact taaggtaggt atttgcggcg aacagggtgg cgatcctgct
     2581 tcggttgagt tctgtttcaa gaacggctta acctacgtaa gctgctcacc attccgcgtt
     2641 cccattgcac gcctagccgc tgctcaagca agtattaaat acggaaagta attctggtat
     2701 aatatttgta cgaaaacgcc ctgaaatcag ggcgtttttt tatacttttg cgtattacaa
     2761 tattttttat gtatttaaaa ttaaggtatt acctgtttat acccttactc ttatttatag
     2821 ggaaaacgaa tataacattt ggacaagatc ctgtaatcga ttcagcatgg gttatatcaa
     2881 cctacaacta ccttttaatg gttgaagcgg attcaggcaa tattgaaaaa gttagaacgc
     2941 tattatacct tggagccgac ccaaacacaa gcgatgaaaa cggtgtaacc ccattgatgt
     3001 ttgcagttca atcgggcaac atagatttag tagaattgct cattgataat ggggcaaatg
     3061 taaacgctta ccctaccaat ggcaacacgg ctctccatgc tgctgttata gctgcaaacg
     3121 atagtgttgc tgagttgctt attaaaaagg gggcagatgt aaatgccaaa aatgtgaaag
     3181 ggcttacccc tctgcatttt gctgtgtggt atggactccc atacctcacc gacatcttaa
     3241 tctactacgg ctccgacctt aacgctattg attaccaagg aaatacaccg ctaatgttag
     3301 cagtattcag cggggcaaag ctaagcacaa ggttgctcct tgagaatggc gccaacccaa
     3361 atatacccga taataaaggg gtgacaccct tgatggttgc tgcacaattc aacgatactg
     3421 ttatctgcaa ctaccttact acctatggtg ctgatttatc gctctgcgat tccaggggtg
     3481 ctaatgccct ttgctatgct attgccaata aatcgaacga aattgttaaa tgtttgagcg
     3541 aatttgatgc ccttaagttc cctcttaaaa agagttatta ccaaattgcc cgcgaataca
     3601 acaataagga agccatgcag ttttttgcca gtaatggaat taaaactaaa atcaaaccat
     3661 caattacttc agtttacttt ggcataggaa caagcttttc aaaacatgag tttctattag
     3721 gctatcattt gggggttttt gaacaaataa cccaaacaga gttctcgctg ggttacctct
     3781 accggccatc tgcaactgcc tcgctaacca ctatcgacgg acaactttac cagtattggg
     3841 aaaagagaaa catactacag ctaaacatcc gcaaatacta taacctgttt agctacaaag
     3901 ggaaaaacat tatttcgttt gcggcaattg gtgcaggacc agcgttccgg aactacaggg
     3961 gaacatcctc gaacccgaaa acacatttta caaccaactt ttctgggggt ctatcgataa
     4021 aaggtgatgc aatggagtat tcgataggtg ttgggtataa tttttataaa gagcttggag
     4081 tatcacccta tttctacacc atatcagcca aaatccattt taatgtgtta aatcctaagg
     4141 ttgtatacaa aaatgtaaaa catgttcagt aagatattta agttactaac cattttgcta
     4201 attgttctgg cgccctactc ctgtgaggaa atggagttct ttatagttaa ctgcgatgag
     4261 tgttatgaat tgaaacctga caaaattgat gttcgcctaa aattcaccat taacaatgag
     4321 aaccaagtag tttactttga actatacagt ggaaatcctg aaaacggagt tttgtttttt
     4381 agtggaaata ctaccataag cgaaaaagta ttccagttcg atgccaactc gtactactca
     4441 ataaaagcgt attacacaag taaaggaagg caaataattg taattgatgc aactacagtt
     4501 aaactaaaat atgataagtc gtcgtgctcc agcccctgtt acacaatttc aggcgatata
     4561 cttgatgctc gcctcaggta ttaaaacatg ttttttagca atgttttaaa accatgtttt
     4621 caaactacta aaaaccaacc gataattcaa aacaatatta gttacttcgc cgtaagtatt
     4681 agtgtttagc aagtatcagt ataaatataa aattgattac ggtgaagaga caactgctta
     4741 tcagggatgt aacccttaga gatggacagc aatcgctgtt tgccacccga atgactcaga
     4801 aacaggttga aagggtatta ccttactacc gtgaagccgg gttttacgcc atggaggtat
     4861 ggggtggtgc cgttcccgac tccgtaatgc gatatctgaa cgaagatcca tggtataggc
     4921 tcgaaagcat taaagctgct attggcgata cttcaaaact cacggccctt tccaggggac
     4981 gcaatctttt tgggtacaac ccatatcccg aagaggtgat tgaaggattt aaccgcaatg
     5041 cggttaaatc ggggatttcc atcatgcgta tattcgatgc ccttaacgat acagagaata
     5101 ttcgcagcac cgtaaagtat gtaaaggaaa atggaggcat ggccgactgt accgtttgct
     5161 ttaccgttga ccctaaattt tcccgtaagg ataggattaa agccatgcta agcggtaaaa
     5221 ccttaccccg taaaatcttt actgaaaagt attatatagc taaggccaag gagctcgagg
     5281 ccatgggtgc cgatatgatt accctaaaag atatggccgg attggttacc cctgagcagt
     5341 cggctgcaat tatttcggcg ctaaaacgcg agattaaagt gcctatcgat ttccatacgc
     5401 actgtacacc gggttatggt ttggcatcaa cccttacagc cattgtaaat ggggttgata
     5461 ttgttgatac tgccatatgg aattttgctg gtggtccagc agcaccagca tttgaactta
     5521 ttcagatttt ctgcgataaa atggggatag atactggtgt taacctgaag gctgtagcca
     5581 gtattaacgc agaacttaaa accattcgca cagagctaaa ggaacttgac acctacgagt
     5641 tacccattga ctttgatata accagctata atctccctgc acgtatcgat aacctgtttg
     5701 agcaagccat tctgtttgcc cagtcgggca agtacgatgc cctacttgat gcttgccagg
     5761 ccatagagcg ttactttaat ttccccgaac ccgatgagaa cgttaaaaac gctgagattc
     5821 cggggggaat gtacaccaat atgctggcac agctcaagca gctaaagctt gaaaacctac
     5881 ttccaagggt gctagaaatc atcccaaccg taaggctcga cgccggttgc cctccgctgg
     5941 ttacacccac cagccagatt gttggtgcgc aagccgtaaa ctgtgctatt gacgagagta
     6001 aaggacagcc attctacacc aataaatcca tacagttcgt aaaccttgtt aaagggatat
     6061 acgggagaac tcccttacca attgacccgc aattccgctt gaagattgcc ggcgtagccg
     6121 aggaaacccc ttacgacaca tctaactaca aaaaacagcc aaacccagcg ttccctgaat
     6181 atggtgagaa tgtgaaattg gccatgaacg agaaggaaga attgctgctt gagctattcc
     6241 ctaccgtagc ggaaaaatat cttcgcgata aagtggtaaa ccattatgag tcaattaagc
     6301 gccagaagga ggaagaggtt cagcgtaaaa ttcaggagga aaaacaaaag tactactcaa
     6361 tgaccgagga acaacgctgg aaaaggcttc tggatggatt acagcaatat ttctgttaac
     6421 ttcaataggt ttaggttaaa agttaggttg aaaaaaaggg gctcttgccc cttttttatt
     6481 ttcgttctat ttcgttgttc atgggtgtta tatcaatact cgtgtcgtaa tcgcccagca
     6541 aatgcttgca gaagtaatcg ccggtaagcc agaaatggta ctcggtcatg tcgccatagc
     6601 catgtcgctg gccagggtac atgaagaaat cgaaacgttt attggcctta atcagagcat
     6661 tggcaagcct aatggtgttt cccggatgaa cgttattatc tatttcgcca gtagtaataa
     6721 gtagatgccc tttaaggttc ttagcaattt cagagttctt atcgatgttg tactcaaacg
     6781 ttacattacc ggccgtatcg gcaatttcct taacgccatg gtgtttttca ctccaccagc
     6841 ggttgtagat gttattctcg tggttgccgg cattagccac tccaacctta aagaaatcgg
     6901 ggaaggttag catggctgct gcagtcataa aaccgccacc tgaatggcca tggataccca
     6961 cacggttaat atcaatatat ttatgtcgat atgccaactg ctcagtagca gcctttttat
     7021 ccgctaagcc atagttgcgc aggttgccat agccatagtt atgataccac tttgaacgag
     7081 cagggtgtcc accccgatta ccaacggtaa taactataaa tccgaactgg gccagcctat
     7141 cggttctatc cattcgtgcg gagaacgact tattcaccgc ctcggtctgt gggccagggt
     7201 acacgtaaag gataatgggg tattgtttag tagaatcgaa atcgaaaggt ttgtacatta
     7261 cgccatacaa atcggtaatg ccatcggcag ctttaacggt aaatggctcg gggaatttgt
     7321 agccagcctc gaatagcagt gttaaatcag ccttttcaag gaaaagtaaa tcgttgccct
     7381 gattatcaat caatttggac tccggaatag tattaacccg cgaatagttg ttcactatat
     7441 actgctgatt atcgtccatg ctgatggaat ggtcaaaatt acccttattc agcagcctta
     7501 ggccagtacc atcaataccg atccgataca gatgaaggta gtatggatcc tcattgggtt
     7561 cacgtccatt tgcggtgaag aacagcgttt tggtggtttc gttgtagcca acaaggcgct
     7621 cgcaatggaa tggtccactg gttatctgcc ttatgagatt tccctggtta tcgtacaggt
     7681 aaaaatgccc ccaaccatcg cgctccgacc agtgaataat ctctttttca ttattaatca
     7741 acgcaatgcc attgaaatcg atataagtgt ttaagcgctc ctctataacc gttttaacct
     7801 cacctgtttt aatatcaaca tagcaaacat caatccgctt gaggtcgcgc gatgtacgct
     7861 ggaagtagag cctatcgttg tacttggaga gccatcgggt tgggacatag tcatcgtcgc
     7921 gttcactggc caatcgacgg taattcagga ttgaaacatc ctgatcctta aatgctttga
     7981 tatcgggttt tatcacctta tccctattat tcatatcaaa aacgagtatc tcgtcctgtg
     8041 gggcttcctt ttcacctggc atgtggtact tataggtttc cagggttggc ctgggattgc
     8101 taagagtctt gatcacccat aaatccttaa ccttacgctg gtcggtacga accatggcga
     8161 agtaacgcga gtcgtgcgac catgtaatgt aagctggctt acggttgtcc ttattcttta
     8221 cctcctctac attatcctcc tccttggtat tatacccgta gctatagtac ttctcaccat
     8281 cagtggttag ctgatgctca acaatggtgg aatccttctc gtttttctta gctttttcgt
     8341 agttctcctt gtccatccag aatagattgt ggttcttgct gtaaaccaca tactcgccat
     8401 taggcgatat atttgcccac tcaggatttt cctttggttt cttataatcg tcgagcaggg
     8461 ttagcttgcg ggtgttcaaa tcgtactcaa agtagtacgt tttcttgtcc tttttggcct
     8521 tggctttatc gtcctttttc tgcttcatgt cattctcctc atcgtcggtt ttatcttcat
     8581 cgaccaacgt gcttttcacc tcaaactgaa tgacagtctc gtttttaatg aacttgattt
     8641 tttgtatagg tagatgctgc gcatcgaacg gctccttggt gagccttgag agctgagcag
     8701 ccatatcaac attatcgaac agcaacgact tgctcttttt tgccggatca actatgtaat
     8761 agcttttgcc cgaaggggtt tcgtaactat accagaaacg cgtactcttc ttaagccagt
     8821 gggcatcaac gctggtacta aaaaccattt tatcaatttt ttttggcgaa aacctggcgg
     8881 gcagatcgta attagcttta gtaacatgta caggttggct aatcacaagc aatacgtttg
     8941 ccattactgc caatagtaac agaaaaagct ttttcatgat tttagagttt tgggttgttt
     9001 tactaacttt gattacaaca gctaagctga gtttaattaa acttcgaaca aattgaaata
     9061 tcaaacaaat aaaaaaatga aaaaactatt tattcttctg ctttgtatct atccactgct
     9121 cggaaacgcc caatactttc cggttacaat agctgagggt agcaactttc agtcaacatc
     9181 aacctatgcc gatgttatga actacatcaa gatgcttgag aaagcatcga agcacataaa
     9241 aaccgaaacc attgctacca gcattgaagg acgcgaaatc ccgctaatgg ttgtggcaaa
     9301 cccaatgccc aaaacacccc gcgatattgg taaccggatt gtgatatata tacaggctaa
     9361 catacatgcg ggggaggtgg aaggcaagga agcatcgctg atgtttgttc gcgacttgct
     9421 taaaaacccc aataatccac tattcaaaaa cattgttctg ctggtttgcc ctatactcaa
     9481 tgccgatggt aacgaatcca tcagtccgaa aaaccgcctt tggcagaacg ggcctgttaa
     9541 cggtgttggc gtaaggcata acgggcaaat gctcgacctg aacagggatg ccatgaaact
     9601 ggaaactccc gaaatggttg gcgtagtaac caacgtatta aaccgctggg atccttccat
     9661 tgttatggat tgccacacca ccaacggatc ctaccatcag gagccggtta cctttacctg
     9721 gatgatgaac cccaacggta atcgcgaact cataaactac atgcgcgata ggatgatgcc
     9781 ctgggtatcg acccagctat cggtcaagta taaaacgcta aactgcttct acggtgagtt
     9841 tatcgaccaa aaggattacg aaaagggttg gatatcgtac gcttctgaac cccgctactt
     9901 caccaactac attgggttaa ggaataggct tagcattttg aacgagaact acgtttacgc
     9961 cccattcgac gaacgggttt acgggtgcta taacctgatt gcctcagttt gcgattacgc
    10021 catgcaaaac agcaaggaaa taaaggattt gcttgccaaa gccgacagag aggctattca
    10081 gtttcccaaa accacacccg ctcctgagtt tgccataacc tacgagggta ggcctacgcc
    10141 ccagtgggta accatcctca cctacgaggc cgaagcctat accgacgagc agggacggga
    10201 acgcttccgc aaaaccgacc gtaagcgcac ggttactgtt ccctacattg ccgactacta
    10261 ccccacctca accattccgc tcccctttgc ctacattgtt agaaacgaca aagtggttac
    10321 caatctcctt aaaaatcacg gaatacaatt ccaaacccta accgatacgg taaccctaaa
    10381 tgtagagcag tttacaatcg actcgctaaa gccagcccct cgaattaacc aggggcacta
    10441 taacagcaat atctttggca aatacgttaa ggccaatagg ctattcacaa ccaactacgt
    10501 gatggttaaa acctcgcaac cccttgggca actggctgct tacctgcttg agccacagtc
    10561 ggacgatggg ttggtttatt ggaatttctg ggaccgttac cttgtgccac aatggggcaa
    10621 caactacaac ccggtaccgg ttttcaggat aaccgatgag aaagagtttg gcagggtaa
//
LOCUS       DAOOWJ010000091        10582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6261_length_10582_cov_7.776575, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000091 DAOOWJ010000000
VERSION     DAOOWJ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10582
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6261_length_10582_cov_7.776575"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..2730
                     /locus_tag="PLW22_08920"
     CDS             418..2730
                     /locus_tag="PLW22_08920"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOU74864.1"
                     /translation="MKLLIAVFKRFFFLLGLPLLVLTGYAQGVVENNPLFDDSEVARI
                     DITINPTYLQSILQAGNEESNTEYPATFSFTSSKFTAVVENAGFRLRGNTSRYAQKKS
                     FKVSFNTFEPGNKLKGIEKLNLNGEHNDPSIVRSKLVWDLMGEIGLPAPRANHVRLYI
                     NNQYYGLYINVEHIDENFVKARFGNNDGNLYKCLYPADLTYRSSDPNAYKFVQNGYRV
                     YDLKTNTEQDNYSDLAQLIDIINRTSISDLPAKLEPIFDVNNFLKYLAVEALTGNWDG
                     YSYNKNNFYLYRNTATGRFEFIPYDTDNTFGIDWFNIDWATRNVTTWVSSQARPLTKN
                     ILAVDVYRKRYYFYLKQLINGAFSTNSIQSKAIALRSKIEAYATEDPFRPLDYGWSSS
                     DFYSSYFNALGGHVKYGLIPYVTKRIQYANSQFSIGDIPPIVSNVSWLAYGYKVPVTV
                     FADVDDEEKANIKLYFKADDSNWQSFEMQNTNRNHYISTLGPWSKPVKAISFYLECTD
                     KSGKTTREPLIGEYSVKFNQIAIPICINEVMTSNQSTYPDEYGNYSDWVELYNYGKNP
                     ISLQGMSISDSLGKPGKWALPSITINPGEFLLLWADGEPEKGNNHLPFRLSNQGEEIG
                     LFTSKTDGYKLIDGYQFGKIAKNESFGYYPNAVGLPQALLSPTPGKSNVYTSKRIDMV
                     LPEIIAYPNPFTESVTITLSYQPAYDYTISIVNLQGITVLEHYMKKDDNPKLTWRPQN
                     LSKGTYLIVFTIHGKQNQLIKPQKVIFEKF"
     gene            2735..3439
                     /locus_tag="PLW22_08925"
     CDS             2735..3439
                     /locus_tag="PLW22_08925"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOU74865.1"
                     /translation="MIRNKLALIFASTLLIVTNASSQVARTLWSGAELQLPFSKAFRV
                     TFSPELRLTDEKRIDDFILEAEAQFRIHKLLQPTFSYRYYRFYDDPGEYFNGQRLGAG
                     INSSVELGRFKLTNRIYYFHRLINRYGYGYSIETRRELREGIKITYNIRRTKLEPFVQ
                     AEIYYDISPDRNHEFSRVRIRTGLEYPFSKRSSIDLFFQMQDKLNTSNPLRTYTIGIF
                     YNYKLPTPKPKSVDIE"
     gene            3498..5459
                     /locus_tag="PLW22_08930"
     CDS             3498..5459
                     /locus_tag="PLW22_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU74866.1"
                     /translation="MISVNNLTLSFGGFDLFKGISFQLNDNEHVGLVGKNGAGKTTLL
                     RLIAGELQPTKGSISLPSDIRLGYLPQQMKLASGKTVIEETRKAFAETIQIKNDINHI
                     SEQISHRTDYESKEYLELINKLTELNDRYAILEGDSADARAEQTLLGLGFKRADFSRQ
                     TTEFSGGWRMRIELAKILLQNPQVLLLDEPTNHLDIESIQWLEDYLKDFQGALLLISH
                     DRAFLDNVTQRTIEISLGNVYDYRVPYSQYVELRRERREQQMAAYRNQQKLIEDTQEF
                     IDRFRYKATKAVQVQSRIKMLERMDIIEVDEEDSATLNIRFPEAPRSGDVVIEAKEVS
                     KSFGNKHVFSDVHFTIERGQKVAFVGRNGEGKTTMSRIIVGELPYDGHLKIGHNVTIG
                     YFAQNQDELLDGSLTVLETIDHVAVGDIRTRMRDILGAFLFRGEDVDKRVSVLSGGER
                     NRLAMAKLMLQPYNLLVLDEPTNHLDMRSKDVLKNALQAFNGTLILVSHDRDFLNGLV
                     DKIYEFTDGKVKEHLGGIYDFLQRKKLQNLKELERKQVEQKAKNSTSANSEQKIQWEE
                     RKELDRQIRKLESQVEKLEKEIGMLESTIAETDTKLSNPTAHGIDLSDKDFFKKYESH
                     KTELMGKMEQWENLLAQVEELKTKRNNTN"
     gene            5462..6202
                     /gene="rlmB"
                     /locus_tag="PLW22_08935"
     CDS             5462..6202
                     /gene="rlmB"
                     /locus_tag="PLW22_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOU74867.1"
                     /translation="MENSENLIFGIRAVLEALNAGAKISKVIVRQGLTGNLASELMGL
                     LKHNNIPFQYLPNEAFTRYKDKNHQGVIAFTSPIEVYRVEDILPQLQEKTNSPFLLIL
                     DSITDVRNFGAIVRTAECAGVDAIIVPAKGAANIGGDAIKTSAGALYNIPICRVGSLK
                     MLSYQLRDYGYRIVSASERGEKLLYEANLTGPIAIVMGAEDTGIHPEVLRLSHEIVRI
                     PLKGKIESLNVSVATGIVLFEAVRQRTL"
     gene            complement(6197..7120)
                     /locus_tag="PLW22_08940"
     CDS             complement(6197..7120)
                     /locus_tag="PLW22_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU74868.1"
                     /translation="MVKSKNVALVLGSGGARGLAHIGVIEELTRRGYSITSISGSSMG
                     ALVGGLLAANRLDDYKNWILKLDKIDILTLIDFTFSSKGIVKGQKVFEKMQELNMVPD
                     VNIEDLPLPYTAVAVDILSNQLIVFNSGKLQTAIRASISIPSVFTPLPYNGTLLVDGG
                     VLSPLPLEYVRRTKNDLLVAVDLNAIIPYDLAVPTNKQSKNSWVKDNPKIEQLVAKWD
                     KFFGHNAAAKESEVKAKQSPKSIGYFDLAMRSVQLMQSQLTQYAIKVTPPDVLVPISK
                     NSATVFEFYKAAELIEYGRLQCEKVLDEKGL"
     gene            complement(7200..7673)
                     /locus_tag="PLW22_08945"
     CDS             complement(7200..7673)
                     /locus_tag="PLW22_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOU74869.1"
                     /translation="MSEHEKFMDEAIRLAVDGVMAGDGGPFGAIVVKDGKIVGRGNNH
                     VTSTNDPTAHAEVMAIRDACKNLNTFQLNGCVLYTSCEPCPMCFGAIYWARLDKVYYS
                     ANRHMASYHGFDDSFIYDEVNLSFDRRRIPFIHVSPEKGVVPFREWDNKENKVKY"
     gene            complement(7786..9063)
                     /locus_tag="PLW22_08950"
     CDS             complement(7786..9063)
                     /locus_tag="PLW22_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOU74870.1"
                     /translation="MEGVEEQVIRKYLFLENLESWFLSQGVSETFAQVFKVVISLATI
                     LILALIADFIAKRIFLTTMTSVARRTKTDWDDVLVEKRFFHRLAHFAPAIVVYLTLGI
                     ALYDYSPKVTLFLQALTKIYMIFIAISVFNAFLDTVNEIYQELPYAKNRPIKGYLQVL
                     KIIIYVFAGILIIGIIINKNPASILVGLGASTAVLMLVFKDVITGLVASIQLSANEML
                     KLGDWIEMPGRNLDGTVVDISLTTVKVQNFDNTITTVPPFALINESFKNWRGMEESAG
                     RRVMKSIIIDSKTVKFCDKELIQKLSNINLIASKLKDIDVDKLHETDPIQLMNAGAQT
                     NLNLFRQYVQEYLNHHPEVNKHLTLVVRLKQPVEYGIPMEIYCFLKEKRFVQYEIIQS
                     EILDHIMAALPVFDLKVFQRMSSDDIRAIAKGN"
     gene            complement(9108..9473)
                     /locus_tag="PLW22_08955"
     CDS             complement(9108..9473)
                     /locus_tag="PLW22_08955"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOU74871.1"
                     /translation="MEIQGKLIKKLDQQTGHGKNGAWVKQEFIIETQEQYPRKICISL
                     WGDKVRELDPIQEGSQIKVAINIESREFNGRWYTDVRAWRIEPMSKGATPEQAPELPP
                     FNDNLYQPMEGGSDDDLPF"
     gene            complement(9539..10261)
                     /locus_tag="PLW22_08960"
     CDS             complement(9539..10261)
                     /locus_tag="PLW22_08960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU74872.1"
                     /translation="MKLKNLLVAAIAIPVVVSCQNSNSKVGKVILKSETDSLSYALGV
                     NIAGSFENAKIDEINYKAMARAIQDFFSKDSSKIKMNDEEAVKYIQDFFNRREMRLAQ
                     ENLEASNKFLDENKTKEGVVVDSSGLQYKIIEEGTGLMPTAEDVVKVHYKGTLIDGTQ
                     FDSSYDRGEPAQFKLNQVIPGWTIGLQKMKVGSKFILYIPSELGYGERVRPGSPIGPN
                     QALIFEVELLDIVKENAQDSKK"
     gene            complement(10359..>10582)
                     /locus_tag="PLW22_08965"
     CDS             complement(10359..>10582)
                     /locus_tag="PLW22_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HOU74873.1"
                     /translation="YFDAFAPDDQPEMWSIENFKKLFNALAPNGALVTYSAKGLVKQN
                     LRQAGFNVERLQGPPGKRHMVRAWKTPDH"
BASE COUNT         3135 a         2367 c         2154 g         2926 t
ORIGIN      
        1 ccacgcgata ctattcgcca cgcctgcggc gtggctcatg tggtatcgct tactagaccc
       61 atcgccacgt tccactcccg acccattagc gggtctggct tttatgccat tgcctgcttc
      121 ttcctctcca ttccctctca ggggaaagga ctggggtaaa gccgttttct aaatttaacc
      181 tagacgaccc ctctaaatct cccctgaaag gggagacttt aacggtgatc ccctctcctg
      241 ccaggagagg ggtttggggt gaggtcattt tccacgagca acaaacaacg aactacgtct
      301 ccagccccgt aggggcgtat taccaatgta aagttcagca atcgattgca tggcttttac
      361 ctcaaatcta tttgaaattc ttcataaatc taccgttaat aatacctttg ctatcttatg
      421 aaattgttaa ttgcagtgtt taaaagattt tttttcctat tagggttacc gcttttagta
      481 ctgactgggt acgcacaagg agtagttgag aacaatccgc tgttcgatga tagtgaggtt
      541 gcacgtatcg atatcacaat taatcctaca tacttacaaa gtatactgca ggctggaaat
      601 gaagagagta acactgaata ccctgccact ttcagcttca cttcatcgaa atttacggcc
      661 gtagtggaga atgctggttt caggttaagg ggtaatacct cgcgctacgc ccaaaagaaa
      721 tcgtttaagg tatcgtttaa tacctttgaa cctggaaata agttgaaggg tattgaaaaa
      781 ttgaacctaa acggtgaaca taacgaccca tcgattgttc gctccaagct tgtttgggat
      841 ttaatgggtg aaattggctt accagctcca cgggcaaacc atgttaggct ttacattaac
      901 aatcagtatt acgggctata catcaacgtt gaacatatag atgagaattt tgtgaaagca
      961 cggtttggca acaatgatgg aaatctttat aaatgtctat atccggccga tttaacatac
     1021 cggagcagtg accctaatgc ctataaattt gttcaaaacg gataccgtgt atatgatcta
     1081 aaaaccaata ccgaacagga caactattcc gatcttgcac aacttattga tattattaac
     1141 cgcacatcca tatcggattt gccagctaaa cttgagccaa tatttgatgt taataatttc
     1201 ctgaaatact tagcagttga ggcgttaacg ggcaactggg atggatactc atacaacaag
     1261 aacaactttt acctttacag aaatacagcc accggacgct ttgagttcat accctacgat
     1321 accgataaca catttggaat tgactggttc aatattgact gggcaacacg caacgttacc
     1381 acatgggtaa gcagccaggc gcgaccttta acaaaaaaca tacttgcagt tgatgtttac
     1441 cgcaagcgct actactttta cctaaagcag ctaataaacg gagcattttc aactaattcc
     1501 attcagtcca aggctattgc tctacgatca aaaattgaag cctacgccac tgaagatcct
     1561 tttcgcccac tcgattacgg atggagtagt tccgatttct atagctccta cttcaatgca
     1621 cttggcgggc atgttaagta cggtttaatc ccttacgtaa ccaagcgcat acagtacgca
     1681 aactctcagt ttagtatcgg tgatattccg ccaattgttt ccaatgttag ctggttagct
     1741 tacggttaca aagttccagt tacagtattt gctgatgttg acgatgaaga gaaggctaac
     1801 ataaaactat acttcaaagc tgatgatagc aattggcaaa gctttgaaat gcaaaacacc
     1861 aaccgcaacc actacatttc cactttgggg ccatggagca aacctgttaa agctatcagc
     1921 ttttaccttg aatgcaccga taagagcggt aaaacaaccc gcgagccact catcggtgag
     1981 tattcagtaa aattcaatca aattgcaata cccatttgca ttaacgaggt gatgacatcc
     2041 aaccaatcca cctatcccga tgagtatggc aactactccg actgggttga gctttacaac
     2101 tacggcaaaa atcccatatc gctacagggc atgagcataa gcgatagctt gggtaaaccc
     2161 gggaaatggg ctttgcccag catcactatt aaccctggcg agtttctgct tctttgggcc
     2221 gatggtgaac ccgaaaaagg caataaccac ttacctttcc gactcagtaa ccaaggcgaa
     2281 gaaattgggc tttttacatc taaaaccgat gggtacaagc tgattgatgg ttaccaattt
     2341 ggcaaaatag ccaaaaatga aagttttggc tactacccca atgctgtcgg cttgcctcaa
     2401 gcactgctta gcccaacacc cggaaaaagt aatgtgtaca catccaaacg gattgatatg
     2461 gtgttacctg aaattattgc ctaccccaac ccatttactg aatctgttac aattacccta
     2521 tcgtatcaac cggcctacga ctacactatt tcaatagtta accttcaagg aattaccgtg
     2581 ctggagcatt acatgaaaaa ggatgataac ccaaagctaa cctggcgccc acaaaacctt
     2641 tcaaagggta catacttaat agtttttaca attcacggca agcaaaatca acttattaaa
     2701 ccgcaaaaag ttatttttga aaaattttga attgatgata cggaataaac tggcattgat
     2761 ttttgcatct actttactaa tagtcacaaa tgctagctcg caggttgctc gcacattatg
     2821 gagcggtgct gaactccagc ttcccttttc aaaagcattt cgtgttacat tctcccctga
     2881 gctaaggcta accgacgaaa agagaattga tgattttatt cttgaagccg aggctcaatt
     2941 tcgtatccac aaactcctgc agcctacttt ttcatacagg tattacaggt tttacgatga
     3001 tccgggtgaa tactttaatg gtcaacggtt aggagcaggt ataaactcat cggttgagtt
     3061 gggtcgattt aaactgacca atcgcattta ctactttcat aggctaataa accgttacgg
     3121 ttatggctac tccattgaaa caaggcgcga actccgcgaa ggtatcaaaa taacatacaa
     3181 cataaggaga acaaagcttg aaccctttgt gcaggccgaa atatactacg atatttcacc
     3241 cgaccgcaac catgaatttt caagggtcag aatcagaacc ggtttggaat accctttctc
     3301 aaagcggtcg agtatagacc tgtttttcca aatgcaggat aagctgaata caagcaaccc
     3361 actccgaacc tacactattg gtatttttta caactataaa ctacctacgc caaaacccaa
     3421 atcggttgat atagagtaaa tgtattatta accattttaa tgtattacct ttgcagcaat
     3481 tttattgctg ttttacaatg atttcggtta ataatcttac gcttagcttt ggtgggttcg
     3541 acctatttaa ggggataagc tttcagctaa acgataatga gcatgttggg cttgttggaa
     3601 agaatggagc aggtaaaacc acattgctaa ggctaattgc tggcgaacta caacccacaa
     3661 agggaagtat ctcattaccc agtgatatta ggttagggta tttgccccaa caaatgaagc
     3721 tggcctcggg aaaaaccgta atagaggaaa cccgcaaggc tttcgccgaa acaattcaaa
     3781 taaaaaatga cattaaccac atctcggagc aaatttcaca ccgcaccgat tacgaaagca
     3841 aggagtacct tgagcttatc aataagctca ccgaattaaa cgaccgctat gccatacttg
     3901 aaggcgatag cgccgatgca cgggcagagc aaaccctttt gggcttaggt tttaaacgag
     3961 ccgattttag ccgtcaaacc actgagttta gcgggggatg gcgaatgcgt attgagctgg
     4021 ctaaaatatt actccagaac ccacaggttt tgctcctcga cgagccaacc aatcatttgg
     4081 atattgaatc gatacagtgg ttggaggatt accttaaaga ctttcagggt gccctactgc
     4141 ttatttcgca cgaccgtgca tttctggata atgttaccca gcgaaccatt gaaatctcgc
     4201 ttggcaatgt ttacgattac cgtgtaccct actcgcaata cgtggagttg cgccgtgaac
     4261 ggcgcgaaca gcaaatggcc gcctaccgca accaacaaaa gctcattgag gatacccagg
     4321 agtttattga caggttccgt tacaaggcca ccaaggctgt tcaggtgcaa tcgcgtatta
     4381 agatgcttga acgaatggat attattgagg ttgatgagga agatagtgct accctgaata
     4441 tccgtttccc cgaagcaccc cgctcaggcg atgtggttat tgaggccaag gaggtttcga
     4501 agagctttgg aaacaagcat gttttttccg atgtccattt taccattgaa cgcggccaaa
     4561 aggtagcctt tgttggccgt aatggcgaag ggaaaactac catgagccgc ataattgttg
     4621 gtgaactccc ttacgatgga cacctcaaaa ttggtcataa tgttaccata ggttactttg
     4681 cccaaaatca ggatgagctg ctcgatggga gcctcactgt acttgaaacc attgaccacg
     4741 tggctgtagg agatattcgc acccgtatgc gcgatatact tggcgcattc ctcttccgtg
     4801 gtgaggatgt tgataaacgg gttagcgtac tatccggagg tgaacggaac cggcttgcca
     4861 tggcaaagct aatgcttcaa ccctacaacc tgcttgtact cgacgagccc accaaccacc
     4921 tcgatatgcg atcgaaggat gttctgaaaa atgcccttca ggcatttaat ggaaccctaa
     4981 tccttgtttc gcatgaccgc gacttcctaa acgggctggt agataaaatc tatgaattta
     5041 ccgatggaaa ggtaaaagaa caccttggcg gtatatacga ttttttgcag cgcaaaaagt
     5101 tgcaaaacct aaaggaattg gaacgtaaac aggttgagca aaaagcaaaa aactcaacca
     5161 gcgcaaactc cgaacaaaaa atacagtggg aggaacgcaa agaactcgac cggcaaatcc
     5221 gcaagctgga gtcgcaggtt gagaagctgg aaaaggaaat cggaatgctg gaaagcacta
     5281 ttgccgaaac cgatactaag ctatcgaacc ctacagccca tggcattgac ctatccgata
     5341 aggatttttt caaaaaatat gaaagccata agactgaact tatgggaaaa atggagcagt
     5401 gggaaaatct acttgctcaa gttgaagaac taaaaacaaa acgaaacaac acaaactaac
     5461 aatggaaaac tcggagaacc taatatttgg cataagagcc gttttggaag cacttaatgc
     5521 tggagcaaaa attagcaaag tgatagtacg ccaagggctt accggcaacc tagcatcaga
     5581 acttatggga ttgctaaagc ataataacat tccctttcag tatctaccca acgaggcttt
     5641 tactaggtat aaggataaaa atcatcaggg ggttattgct tttacctcac caattgaggt
     5701 ttatagggtt gaagatattt tacctcaact acaggaaaaa accaactcgc cattcctact
     5761 tatcctcgac agcattaccg atgtacgcaa ctttggtgca attgttcgaa ctgccgaatg
     5821 cgcaggcgtt gatgccatta ttgttccagc caaaggagcc gccaatattg gcggcgatgc
     5881 cataaaaacc tcggctggcg cactatacaa catccccatt tgtagggtag gtagcctaaa
     5941 aatgctaagc tatcagctac gcgattatgg ttataggatt gtttcggcaa gcgaaagggg
     6001 cgaaaagttg ctctatgaag ctaaccttac cgggccaatt gctattgtta tgggagcaga
     6061 ggatacaggc atacaccccg aagtgctaag gctatcgcat gaaattgttc gtataccatt
     6121 aaagggtaaa attgagtcgc taaatgtttc ggtggccact ggcattgttc tgtttgaagc
     6181 cgttaggcag cgaaccctat aacccctttt cgtccagcac cttttcgcat tgtaagcgtc
     6241 cgtactcaat cagctcggca gccttgtaaa attcaaaaac agtagccgaa ttttttgata
     6301 tgggaacaag cacatcgggc ggggttacct ttattgcata ctgggttaac tgcgactgca
     6361 tgagttgcac cgaacgcatg gctaaatcga agtagccaat actttttggc gattgcttag
     6421 cttttacctc gctttcctta gctgccgcat tgtgcccaaa gaatttatcc cacttggcaa
     6481 caagttgctc aattttaggg ttatccttaa cccagctgtt tttggattgc ttattggttg
     6541 gcacagccag atcgtaaggg ataatggcat tcaaatcaac agccaccagt aaatcgtttt
     6601 tagttcgcct aacatactcc aggggtaatg gcgaaagcac tccaccatca acaagtaatg
     6661 taccattgta tggcaatggt gtgaaaacgc ttgggatgga aatggatgcg cgaatggcgg
     6721 tttggagctt gccagaatta aaaacaataa gttgattact taaaatgtcg actgcaacag
     6781 cagtgtatgg taggggcaaa tcctctatgt ttacatcagg aaccatattg agttcctgca
     6841 tcttttcaaa aaccttttgc cctttaacaa taccttttga tgagaatgta aaatcgatca
     6901 gcgttaggat atcaatttta tcgagcttga gtatccagtt tttgtaatca tcaagcctgt
     6961 tggcagccag caagcctccc acaagtgccc ccattgatga gcctgagata gatgtaatac
     7021 tataacccct tcgggttaac tcctcaatca ccccaatatg ggctaaaccc cgagcgccac
     7081 cactcccaag taccagtgca acattctttg atttaaccat agttaattgc aacaattatt
     7141 tgaataccaa tatttttact caaagaatgc taataaaggc aaaattgagg aacaaccaat
     7201 taatacttta ccttgttctc cttattatcc cactccctaa agggtacaac acccttttcg
     7261 ggactaacat gaatgaatgg gatacgcctg cggtcgaacg ataggtttac ctcatcgtaa
     7321 ataaagctat cgtcgaaacc gtggtacgat gccatatggc ggttagctga atagtaaacc
     7381 ttatcgagac gagcccagta aatggcccca aagcacatgg ggcaaggctc acatgaggta
     7441 tagagaacac aaccgtttag ctgaaaggta ttcaggttct tacatgcatc gcgtatggcc
     7501 attacctcag cgtgagcggt tgggtcgttt gttgatgtaa catggttatt gccacgccct
     7561 acaatttttc catccttaac cacaatagcg ccaaaagggc ctccatcgcc agccattacg
     7621 ccatcaacgg ctaaccttat ggcctcgtcc ataaattttt cgtgttcgct catagcttac
     7681 tattaaatct ctaatttact aagaaaatat tcaacggcaa ttataccaaa tatttattga
     7741 aacagaagcg aaacccaatg gttggattcc gcttccggaa gctaattagt tacctttagc
     7801 aattgctcta atatcatcgc tcgacattcg ctgaaaaacc tttaaatcaa aaacaggaag
     7861 cgcagccatg atatggtcaa gaatttccga ttgtataatt tcatactgaa caaatcgttt
     7921 ttccttgagg aagcagtata tctccatggg aataccatac tcaacaggct gtttcagcct
     7981 aacaacaagg gtaaggtgtt tattaacctc agggtgatgg tttaggtatt cctgaacata
     8041 ttgcctgaaa aggttcaggt tggtttgcgc tccggcattc atcaactgaa tggggtcggt
     8101 ttcatgcagc ttgtcaacat caatgtcctt aagcttgctg gctatcaggt ttatattcga
     8161 cagtttctgt ataagttcct tatcgcagaa cttaacggtt ttggagtcaa taataatcga
     8221 tttcattacc ctacggccag ccgactcttc cataccacgc cagtttttga agctctcgtt
     8281 gattagcgca aaaggcggaa ctgtagtaat ggtattgtca aagttttgaa cctttactgt
     8341 ggtaaggcta atatcaacta cagttccatc gaggtttctt ccgggcatct caatccaatc
     8401 gccaagcttt agcatctcat tagccgatag ctggattgat gcaaccaaac cagttattac
     8461 atccttgaat accaacatca aaacggcggt tgaggctcct aaacctacca gaatcgatgc
     8521 cggattttta ttgattatta ttcctataat caaaatgcct gcaaaaacat aaataataat
     8581 ttttagaacc tgcaggtatc ctttaattgg tctattcttg gcatatggta attcctggta
     8641 aatttcgtta actgtgtcga gaaaagcatt aaatacacta atggcaataa aaatcatgta
     8701 gatttttgta agtgcctgta aaaacagtgt aaccttgggt gaatagtcgt acagggctat
     8761 accaagggtc aagtacacca caatggcagg agcaaagtgc gctaaacggt gaaagaatcg
     8821 tttttcaacc aaaacatcgt cccagtcggt tttggttcgc ctagctacac tagtcatggt
     8881 ggttaaaaaa atccgctttg caataaaatc ggcaattaat gcaagtatta aaatggttgc
     8941 aagcgatatt accaccttga aaacctgggc aaaagtttcg ctaacgcctt ggcttaagaa
     9001 ccacgactca aggttctcga gaaaaagata cttgcgaatt acttgttcct ctacaccttc
     9061 catgttcaag tattttttgt tgatagtttc ctaaagcaaa attacagcta aaaaggtaag
     9121 tcatcgtcac ttccaccctc cataggctga taaagattat cattgaatgg gggaagttca
     9181 ggggcttgtt cgggtgtggc acccttgctc attggttcaa ttcgccatgc cctaacatcg
     9241 gtgtaccacc gaccgttgaa ctcgcgcgat tcaatgttga ttgcgacttt tatttgactg
     9301 ccctcctgaa ttggatcaag ctctctcacc ttatcgcccc aaagcgatat gcaaatcttt
     9361 cgggggtact gctcctgcgt ttcaataatg aattcctgct tgacccaagc gccatttttc
     9421 ccatgcccgg tttgctggtc gagcttttta ataagtttac cttggatttc catttttcaa
     9481 tcgattaaaa aaccgggcga atgatcaacc cggttatgta tgattgacta taattaaact
     9541 acttcttgct gtcttgagca ttttccttta caatgtcaag cagttcaacc tcaaaaatca
     9601 gggcttggtt agggccaatt gggcttcccg gtcgaaccct ttccccgtaa cctaactcac
     9661 tagggatgta aagaatgaac ttagagccta ctttcatttt ttgcaaacca atagtccagc
     9721 caggaataac ctggttgagt ttaaactggg cgggttctcc cctgtcgtac gatgaatcga
     9781 attgggttcc atcaatcaga gtacccttgt aatgaacctt taccacatcc tcagctgtag
     9841 gcataagccc tgtaccttcc tcaataatct tatactgcaa acccgatgag tcaacaacta
     9901 caccttcctt ggttttgttc tcatcaagga acttgttgct tgcctctagg ttttcctgag
     9961 caaggcgcat ctcgcgacgg ttaaagaaat cctgaatgta tttaacggct tcctcatcat
    10021 tcatcttgat tttggaagag tctttactga aaaaatcctg aatggcgcga gccattgcct
    10081 tgtagttaat ttcatcgatc ttggcattct caaaactacc ggcaatgttt acgccgagtg
    10141 catagctcaa gctatcggtt tcagatttta ggataacctt gccaacctta ctatttgaat
    10201 tttggcacga tacaactaca ggtattgcaa tggcagcaac gagtaaattt tttagcttca
    10261 tggtgatatt gatttaaatt agttacttat caataaattc tctgcgaata tactacactt
    10321 tttgaataag aaaaaaatgt tagctttttt tgaagatttt aatgatcggg agttttccaa
    10381 gctcttacca tgtgcctttt accgggaggg ccttgcagcc tttcaacgtt aaagcctgcc
    10441 tgccgtaaat tctgctttac cagcccttta gccgaatagg ttaccaatgc gccgttaggt
    10501 gctaaagcat tgaatagttt tttgaaattt tcaatactcc acatctcggg ctggtcgtca
    10561 ggagcaaaag catcaaagta aa
//
LOCUS       DAOOWJ010000092        10441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6378_length_10441_cov_7.814462, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000092 DAOOWJ010000000
VERSION     DAOOWJ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_10441_cov_7.814462"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="PLW22_08970"
     CDS             <1..1421
                     /locus_tag="PLW22_08970"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOU74874.1"
                     /translation="WSVSNTNKAGGSAYEMKCTYQNVNPGTTRLITPAINTSGVSQVT
                     LTFRHMLDAYSTGVTLRVQTSNDKTNWTNTSWSVATSSTNINATSVTVNITTNLNSAT
                     TYIAFVAEGNLYNIDYWYIDNVSVAAGSGATTPTVTTGSVSNITSSTATVAGNVTADG
                     GATVTERGICYSTSQNPTTANSKVASGSGTGSFSANLSGLAANTTYYARAYAINAQGT
                     SYGSQVSFTTTTSTVTYCASKGSNSSYEWIDLVQFAGINRTSGNDGGYKDMTSLQATV
                     ARGTTYTIYFSAGFKSSSYTEYWAVWIDYNKNGTFDTNEKVVSGSSSSSGTLSASITI
                     PTTATLGVTRMRVSMKYNAAPTACETFSYGEVEDYSVNITSGTSAPSTDNPFAEELGN
                     EKVDIFTIYPNPASEKINVVLNGIEGEVSARIYDMRGAVVKFQMLTDRNNTIEINDLA
                     PGVYMISIDDEKQPITKQFIKK"
     gene            1666..2061
                     /locus_tag="PLW22_08975"
     CDS             1666..2061
                     /locus_tag="PLW22_08975"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU74875.1"
                     /translation="MEFEVTFQGKKQVVAHIGEHDILTDQPVYGGGDNQGPAPFTLFL
                     ASIGTCAGIYVKSFCDQRGIPTDNIKIVQKHQFNPLTHMIERLELEINLPADFPEKYK
                     EAVIKAADQCAVKKHLHNPPQMSVTTKSI"
     gene            complement(2112..4298)
                     /locus_tag="PLW22_08980"
     CDS             complement(2112..4298)
                     /locus_tag="PLW22_08980"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOU74876.1"
                     /translation="MRAIKNLTSWLLLFLISSNVLADEGMWMLNLIGKNISAMQTLGL
                     KLTAEDIYSVNKSSLKDAIVQFDDGSCSAEMVSPNGLFFTNHHCGVDAVQELSTVSNN
                     LLENGYWAKSYAEELPAKGKTALILVDITDVTSDILTGISPSLSNREYFEAINQKMQY
                     LQDSVEAKSGYHVVVRPFFNYNEFYMFRYERYLDVRLVGVPPASIGNFGGDVDNWHWP
                     RHTGDFCIFRIYTSPDGKPAQYSPKNIPLKSKNYLKINLQGVQEGDFAMIIGFPGRTH
                     RYASSYEAMFNREVVANFKRTVWGEMINAIKKAQQTDPAIKVNYTDKHDYLINFYQKD
                     VWQAESMYKYSVVERLAAREDSLKAWANSNPFLYSRYVTSIPVLENYYQTVTQNRWEE
                     LEGAISALSFYPVDVYKNVTACNDFVSAIIMQGKPYSRLSFKRDYIRQEAKLLEKRLP
                     KIFEKYHIYPDVQLYSIAFGALLNNSGEFNNLGMLNAIKKVEDISTSYPYYIMAFFEK
                     SYFTSPENLKKLIKRPYLDSLVKDPLFILYHNYNMLWDSIYNSMYEAKQNYNRAMQLY
                     TKGIMEMNKGKLLYPDANSTPRLTYGKVKGYRPADGMAYKPFTTLDGVMEKEDPNSDV
                     FKVPEKLRKLWERKDYGPYARNGVMPVCFLTDNDITGGNSGSGVLDANGNLIGIAFDG
                     NAEAMACDFMYEPETQRTIVVDIRYVLFIIDKFGESKHIMNELTIL"
     gene            complement(4314..5534)
                     /locus_tag="PLW22_08985"
     CDS             complement(4314..5534)
                     /locus_tag="PLW22_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOU74877.1"
                     /translation="MKRRILVVARYLLAIVFIFSGFVKGIDPWGTAYKLHDYFNAFHF
                     HFLDNLALPLSVLLCAAELYIGLLLLSGVKQKIAAWGAFLFMVVFTPLTLLLAITNPI
                     SDCGCFGDAIKLSNWGTFFKNLVFFAAAAFLFTNRREELSRQNPIKQNLLSLLLLAIA
                     ILPSVHGIRHQPIIDFRPFHIGANLLQYTSIPEGAPHDVYRTILYYQKDGVVKEFDET
                     NYPWNDSTWTFVDSKSYLVEKGYTPPIANFTLYTEDGHNYTDSILYSKGDLILVVIPN
                     IKYLSEPAIARFKTIQKQARDKGVKILGLTSAAGTDLANLENQHGLFFTWLQADEVML
                     KTIVRSYPGMLYLYNGTIIGKWHWRDIPETLFKTEQPLSFLVTASQKKWEYAIASILS
                     LSLILILILFNRNK"
     gene            complement(5546..6238)
                     /gene="tmk"
                     /locus_tag="PLW22_08990"
     CDS             complement(5546..6238)
                     /gene="tmk"
                     /locus_tag="PLW22_08990"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOU74878.1"
                     /translation="MALIVIEGLDGAGKSTQIDLLTQHLKTKSIPCHFLHFPRVDAPF
                     FGDLIARFLRGDLGTIDRVDPYVVALLYASDRMDAAPMIRQWLNEKHVVLLDRYVYSN
                     VAFQCAKLNNPHKINLLREWILKLEFDYYAIPKPNLNIFLNVPFEFTRQRLTEQRIGE
                     SRQYLQGKDDIHEKDLSFQEKVRNIYLQQQQFDPNFVVLDCTDKEGHMLKPEAISSMI
                     LNEIAKRNLLNL"
     gene            6327..7208
                     /gene="folP"
                     /locus_tag="PLW22_08995"
     CDS             6327..7208
                     /gene="folP"
                     /locus_tag="PLW22_08995"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOU74879.1"
                     /translation="MEEKRSVFAPRKTLQFNGRLITIEKPLVMGIINITNDSFFSGSR
                     YRFSHSIARRAKQIVEEGGQIIDIGACSTRPGAKPVSERDEIKRLHKALAAIRRSYPE
                     AIISVDTFRASVAEWAVKEFRVDIINDISAGQMDSKMFETIAELNVPYILMHMQGTPE
                     NMQQNPQYENVIRDLILFFNDKIEKLKQLNVKDIIIDPGFGFGKTIEHNYTILKNLET
                     FKLLELPILVGISRKSMIYKPLNTKPEQALNGTTVLNTLAILKGASILRVHDVKQAAE
                     TIRLLELLNAQPEFQFI"
     gene            7213..8016
                     /gene="cdaA"
                     /locus_tag="PLW22_09000"
     CDS             7213..8016
                     /gene="cdaA"
                     /locus_tag="PLW22_09000"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOU74880.1"
                     /translation="MELLPIPLFIHVRVLDIVDIVIVAFLLYQIYLLIRGTAAIKIFS
                     GIVLLYFIWLVVKALNMELLGNLLGQVIGVGVIALIIVFQQEIRKFLLLLGSRYTTKG
                     KSALESFLLGTDQNEVSQTNLDAIVKACKNLSETKTGALIAITRNSELEVYSQTGDII
                     NAELSSRLLENIFFKNSPLHDGAVIIKGNKIHAARCVLPISENLNLPPKFGMRHKSAL
                     GLTEVTDALVIVVSEETGKISLADNGSIDYGISLTDLKEKLEQKLQAKR"
     gene            8066..9310
                     /locus_tag="PLW22_09005"
     CDS             8066..9310
                     /locus_tag="PLW22_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74881.1"
                     /translation="MIKYQITKSDTKIYGEVTIPPTRPMSNKFLVIKVYKDANLPTGQ
                     GSEIGNEKIINQSIFKEGLKSKSSRTARAIRHIRAFINYFGGEWILSSSNILKDSTTG
                     KIVALLQKYGLKVQFEERIGNPPFRLIGKNLKGKILRVEGSINSKMIEARLLIDPSLN
                     SEEIIELKNFIISQGYSTMTLRALQFMGVNIDWREDEVLIEHQIFDGSELSIEADWSL
                     ASYWYEAVALADKGEIAIKGLKPESFQCDTAVKDLFTRLGVTTIIAENAIILKRKGRV
                     TKQFEHNFKQHPDLAPAAMFTCLALGIPFTISGIDYFHTKDPDRLNAIKAEFQKLGAT
                     INVQLENDVEVLKFDGKSKLKNLKVLECDSQNDHRVAMSLAPYAILGKRVVMNNARIT
                     SKSYPTFWDEIKKLGFEVTVLQ"
     gene            complement(9375..9848)
                     /locus_tag="PLW22_09010"
     CDS             complement(9375..9848)
                     /locus_tag="PLW22_09010"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOU74882.1"
                     /translation="MENLKYAGFWWRFLAYILDAIIIGVIQWIIVIPVLALIGFSAAS
                     AASNGITETEAIGMVGAIVGTAMITYLALIVLMWLYFAIMESSKFQGTVGKIAIGLVV
                     TDMEGNRISFARATGRYFGKILSSLIFMIGYILAGFTQKKQALHDMLAGTLVWKK"
BASE COUNT         3265 a         2147 c         2115 g         2914 t
ORIGIN      
        1 gttggagcgt ttccaacacc aataaggctg gcggatcggc ctacgaaatg aaatgtactt
       61 accagaatgt taacccgggc accacacgcc tgatcacacc tgccataaac acatcaggtg
      121 taagccaggt aacattaaca ttccgccata tgcttgatgc atacagcacc ggtgttactt
      181 tgagagttca aacctcgaac gataagacca attggaccaa cacatcttgg tcagtggcta
      241 cctcaagcac caatattaac gcaactagtg ttactgttaa cattacaaca aacctcaact
      301 ctgcaacaac atatatagct tttgttgccg aaggtaatct ctacaacatt gactactggt
      361 atattgacaa tgtttctgtt gccgcaggta gcggagcaac tacaccaacg gttactactg
      421 gcagtgtaag taacattact tccagcacag ccacggtagc cggtaatgta acagccgatg
      481 gtggtgcaac tgtaactgaa cgcggtattt gctacagcac atcacaaaat ccaaccacag
      541 caaatagcaa ggtagcaagt ggttcgggta ctggatcgtt cagtgcaaac ctcagtggtt
      601 tggctgctaa caccacatac tacgctcgtg catacgcaat taacgcccaa ggcacatcgt
      661 acggtagcca ggttagcttt accacaacta ccagcacggt tacctactgc gcttcaaagg
      721 gtagcaactc cagctacgaa tggattgacc ttgttcaatt tgcaggaatc aatcgtactt
      781 caggcaacga tggtggctac aaggacatga ccagtctgca ggctaccgtt gcacgcggaa
      841 ccacatacac aatctacttc agtgcaggat tcaagagtag ctcctatacc gagtactggg
      901 ctgtatggat tgactacaac aagaatggca cttttgatac taatgaaaag gtagttagcg
      961 gttcatcatc gagcagcgga accctttcgg ctagtattac cattcctaca actgctaccc
     1021 ttggcgttac tcgcatgcgt gtaagcatga aatacaatgc tgcacctacc gcttgtgaaa
     1081 catttagcta cggcgaggtt gaggactaca gcgtaaatat tacaagcgga acatcagctc
     1141 cttcaaccga caatcctttt gctgaggagc ttggtaacga aaaggttgat attttcacca
     1201 tctatcctaa ccctgcttcc gaaaagataa atgttgttct taacggcatt gaaggtgaag
     1261 tatcggctcg tatatacgat atgcgtggcg cggttgttaa attccagatg ctaaccgaca
     1321 gaaataacac aattgagatt aatgatttgg ctcctggtgt ttacatgatc tcaattgacg
     1381 atgaaaaaca gcctataaca aaacaattta tcaaaaagta atagctgcta gagtgtttta
     1441 tgcgaagagg ggtgtcgatt ttcggcaccc cttttctttt ctattaaccg aatatgcaaa
     1501 atggcaatta atttcaaaat cctatcactc ggtaagaatt tccacttaat aatgaacctg
     1561 caattaacac tatttagaaa ttttccaaat aatttatatg atatgtaaac atttacatat
     1621 gttattgtgt tataattttg caaaactaaa aaataattaa aacatatgga atttgaagtt
     1681 acatttcaag gcaaaaagca ggttgttgct cacattggag agcacgatat tttaaccgat
     1741 caaccggttt atggaggtgg cgataaccaa ggcccagctc cattcacatt atttttggca
     1801 tcaataggta cctgtgcagg tatttacgta aaatcgttct gtgaccagcg gggcatacca
     1861 accgacaata ttaaaattgt gcaaaagcat cagtttaacc ccttgacaca catgattgaa
     1921 aggttggaac tcgaaatcaa ccttcctgcc gattttcctg agaaatacaa ggaggccgtt
     1981 attaaggcgg ccgaccagtg tgcggttaaa aagcacctgc ataacccgcc tcaaatgagc
     2041 gtaaccacta agagcatata ataaaaaact cccgctttct gcgggagttt ttatttaaga
     2101 gatatggatt tttagagtat tgttaactca ttcatgatat gctttgattc accgaactta
     2161 tcgataataa atagtacata cctaatatca accacaatgg ttcgttgggt ttcgggttcg
     2221 tacataaagt cgcaggccat ggcttcagcg ttgccgtcaa aggctattcc tatcaaattc
     2281 ccattagcat cgagcactcc gctaccggaa ttaccaccgg taatatcgtt gtcggtaagg
     2341 aagcaaactg gcattacgcc attcctagca tacgggccat aatccttacg ttcccatagc
     2401 ttacgtagct tttcaggcac tttaaacacg tcggaattgg gatcttcctt ttccattact
     2461 ccatcaaggg ttgtaaaggg tttgtatgcc atcccatcgg cagggcgata accctttacc
     2521 ttcccatagg ttaaccgggg tgttgaattg gcatcggggt ataaaagttt acccttgttc
     2581 atttccatta ttcctttggt atagagctgc attgctctgt tgtagttctg ctttgcctca
     2641 tacatggagt tgtatatgga atcccaaagc atattgtaat tatggtatag gatgaagagc
     2701 ggatctttta ccaatgaatc caagtaaggt cttttaatca gtttctttag attttcaggc
     2761 gaggtgaaat aggatttctc aaagaaagcc attatatagt atggataaga ggtgctaata
     2821 tcttcgactt ttttaatggc attaagcatt cccaaattat tgaattcacc actgttgttt
     2881 agcagtgcgc caaaagcaat agagtatagc tgaacatcgg ggtaaatatg gtatttttca
     2941 aagatttttg gtaatcgttt ttcaagcagt ttagcctcct ggcgaatgta atccctttta
     3001 aaacttaatc ggctgtaggg cttgccctgc atgatgattg cggaaacgaa atcgttgcaa
     3061 gcggtaacat ttttatatac gtcaaccggg taaaacgaca gagctgaaat ggctccctca
     3121 agctcctccc agcggttttg ggttacagtc tggtagtaat tttccaatac tgggatactg
     3181 gtaacatagc ggctgtaaag aaaaggattg ctattagccc aagcctttag gctatcctcc
     3241 cgggcagcaa ggcgttcaac aacgctatac ttatacattg actcggcttg ccaaacatct
     3301 ttttggtaaa agtttataag atagtcgtgc ttatcggtat agttaacctt aattgcgggg
     3361 tcggtttgtt gggctttttt aattgcgtta atcatttccc cccaaacggt acgcttaaag
     3421 ttggctacca cctcgcggtt aaacatagcc tcataggacg aagcataacg gtgcgttctt
     3481 cccggaaatc cgattatcat ggcaaaatcg ccctcctgaa ccccttgcag gttaattttt
     3541 aagtaattct ttgatttcaa cggaatgttt tttgggctgt actgagcagg tttaccatcg
     3601 ggagaggtgt atatacgaaa tatacagaaa tcgcctgtat ggcgaggcca atgccagtta
     3661 tccacatccc ctccaaaatt tccaattgat gcgggaggaa ctccaaccaa acgaacatca
     3721 aggtaacgct cgtaacggaa catgtaaaac tcattgtagt taaaaaacgg ccttaccacc
     3781 acgtgatacc cgctttttgc ttcaacggaa tcctgaaggt attgcatctt ctggttaatg
     3841 gcttcaaagt attccctgtt ggaaagcgat ggactgatgc cggtaagaat atcggaggtt
     3901 acatcggtaa tgtcaacaag tataagcgcc gttttgcctt tagccggcaa ttcttcagca
     3961 tacgatttag cccagtaacc attctctaac aaattgttgg aaacggtgct taattcctga
     4021 acagcatcaa cgccacaatg gtgattggtg aagaaaagac catttggcga gaccatttca
     4081 gcactacagc taccatcatc gaattgaact atggcatcct ttaggctcga tttattaaca
     4141 ctgtaaatat cctcagctgt caattttagt cctagcgttt gcattgccga aatgttttta
     4201 cctataaggt taagcatcca catgccctca tcggcaagca cattggaaga aataagaaaa
     4261 agtaaaagcc aactggtcag gttctttatt gctctcatat tggaaatttt gatttatttg
     4321 tttcgattaa acaagatcag aattaatatt aaagatagcg ataatattga agcaatagca
     4381 tactcccact ttttttgtga agcagttact aaaaatgata aaggttgctc ggttttaaaa
     4441 agggtttcag ggatgtcgcg ccagtgccat ttgccaatta ttgtaccatt gtataaataa
     4501 agcataccag ggtacgatcg gactatggtt tttagcataa cctcatcggc ttgaagccat
     4561 gtaaagaata aaccatgttg gttttccagg tttgccaaat ctgttccagc agccgatgtt
     4621 aacccgagga ttttaacgcc tttatcgcga gcctgtttct gaatagtttt aaacctagct
     4681 attgcgggtt ccgataagta tttaatattt ggaattacaa ctaaaatcaa atcaccttta
     4741 gagtaaagaa tgctatcggt atagttatgt ccatcttcag tgtacaacgt gaagttagct
     4801 ataggagggg tgtagccttt ttctactaaa tatgactttg aatcaacaaa agtccatgta
     4861 gaatcattcc acgggtagtt ggtttcatca aactccttta ccacgccatc cttctggtag
     4921 taaagaattg ttctgtaaac atcgtgaggt gccccttcag ggattgaggt gtactgtaat
     4981 aaattagcac caatgtggaa aggtctaaaa tcgataatgg gctgatggcg tatgccatga
     5041 acagagggta aaattgcaat ggcaagaagc agtaatgaaa gcaaattctg tttgatagga
     5101 ttttgccttg atagttcctc cctacggttg gtaaaaagga atgcagccgc agcaaaaaaa
     5161 actaaatttt taaaaaaggt tccccaattc gatagtttaa tggcatcgcc aaaacaacca
     5221 caatcagaga tgggattggt gatagccagc aaaagggtca ggggtgtaaa tactaccatg
     5281 aataggaatg ccccccatgc agcaatcttc tgttttaccc ctgatagtaa tagcaagcca
     5341 atgtacagct cagcggcgca aagcaaaaca gaaagaggga gtgctaaatt gtccaggaaa
     5401 tggaaatgaa acgcattaaa gtaatcatgt aacttgtaag cagtacccca cgggtcaatt
     5461 ccctttacaa accctgagaa aataaaaact attgccaaca gataccttgc aactactaaa
     5521 attctccttt tcattgccat tttctttata aattcaacaa attccgtttt gcaatttcat
     5581 tcagtatcat tgacgaaatt gcttcaggct tgagcatatg cccctctttg tcagtacaat
     5641 ctaatacaac aaaatttggg tcgaactgtt gctgctgtag gtagatattc cgaactttct
     5701 cctgaaatga taggtccttt tcgtgaatat cgtccttacc ctgcaagtat tgccttgact
     5761 caccaattct ttgctcagtt agacgttggc gggtaaattc aaaaggtaca tttaaaaaga
     5821 tatttaagtt gggtttaggt attgcatagt aatcaaattc cagctttaaa atccattccc
     5881 ttaacaggtt aattttatgg ggattgttaa gctttgcaca ctgaaaggca acgttggagt
     5941 atacatacct atcgagcaga accacatgct tttcattaag ccattggcga atcattggag
     6001 cagcatccat cctatccgaa gcatacaaaa gggctaccac ataaggatca accctgtcga
     6061 tagttcccaa atcgccacgt agaaacctag caataagatc gccaaaaaat ggggcatcaa
     6121 ctctaggaaa gtgaaggaaa tggcaaggta tggactttgt tttaaggtgt tgtgtaagta
     6181 agtcaatttg ggttgatttg cctgcaccat cgagcccttc aataacaatt aaagccatct
     6241 gcgaaaaaat taaaaaacaa catctgtaaa cttaacacaa tgtttcgtaa ttttagcatt
     6301 tgaaacattt tgcaagatag gaaaaaatgg aagaaaaacg ttcagttttc gcgccaagaa
     6361 aaacgttgca gttcaacggc agactcataa ccattgagaa acccttggtt atgggaataa
     6421 taaacataac aaacgattca ttttttagcg gtagccggta caggttttcg cacagcatag
     6481 cccgcagggc aaaacaaata gttgaggaag gtggccagat aattgacatt ggtgcttgct
     6541 caacccgacc gggtgcgaaa ccggtaagtg agcgggatga aataaaacgc ctccacaaag
     6601 cgttggctgc tattcgaagg agttatcctg aagccataat ctcagtagac accttccgtg
     6661 cttcggttgc agaatgggct gtaaaggaat ttcgcgttga tataatcaac gatatatcgg
     6721 caggacagat ggacagcaag atgtttgaaa ctattgctga actaaatgta ccctacatac
     6781 tcatgcatat gcaaggtaca cccgagaata tgcagcaaaa ccctcaatac gaaaatgtta
     6841 tcagagatct cattctattc tttaacgata aaattgagaa gctgaaacag cttaacgtta
     6901 aggatataat tattgacccc ggctttggtt ttggcaaaac cattgagcat aactatacta
     6961 tcctaaaaaa ccttgagaca tttaaactac tagaacttcc gatacttgta ggcatttcaa
     7021 ggaagtcgat gatatacaaa ccactaaaca ctaaacccga acaagcatta aatgggacaa
     7081 cggttctgaa cacgttagca attcttaagg gtgccagcat acttagggtt catgatgtta
     7141 aacaagctgc tgaaaccata agattgcttg aactacttaa tgctcaacct gaatttcaat
     7201 tcatttagcc ccatggaatt attaccgata ccgcttttta tacatgtaag agtgctcgac
     7261 attgtcgaca ttgtaattgt agcatttctt ctttaccaaa tatacctttt aattagaggg
     7321 actgccgcca taaaaatttt ttcagggatt gtgctcctgt acttcatttg gctagttgta
     7381 aaagcattga acatggagtt acttggcaat ttgcttggcc aggtaattgg ggttggtgtt
     7441 attgccctca ttattgtttt ccagcaggag ataagaaagt ttttgttgct gcttgggtcg
     7501 cggtacacaa ctaaagggaa aagtgccttg gagtctttct tgctaggtac ggatcagaat
     7561 gaggttagcc aaacaaacct cgatgcaata gtaaaggctt gcaaaaacct atcggaaacc
     7621 aaaacagggg cactaattgc cattacccga aactcagagc ttgaggtata ctctcaaacg
     7681 ggcgacatta ttaacgcaga actctcaagc cggctgcttg aaaatatttt cttcaaaaat
     7741 agcccattac acgatggggc ggtaataatc aaaggcaata agattcatgc tgcccgatgt
     7801 gttttgccaa tatcggagaa ccttaattta cccccaaagt tcggaatgag gcataaatcg
     7861 gccttaggat tgacagaggt tacggatgcc ttggtaattg ttgtttcaga agaaacaggc
     7921 aaaataagct tagccgacaa tggcagtatc gattatggca ttagccttac tgacctaaaa
     7981 gagaaactag aacaaaagtt acaagctaaa cgataggata ataaagttta tgttacattt
     8041 gttcaacacc taaatcttat cagcaatgat taagtaccaa atcaccaaat cggacacaaa
     8101 gatttatggt gaggttacaa taccccccac ccgaccaatg tcaaataagt ttttagtaat
     8161 taaggtttac aaagatgcaa atcttcccac aggacagggt tctgaaattg gcaacgaaaa
     8221 aattattaac caaagcatct ttaaggaagg actaaagagc aagagtagcc gtacagcccg
     8281 agcaattagg catatcaggg cctttatcaa ctactttggt ggcgagtgga tactttcatc
     8341 gtcaaacatc ttaaaggaca gcaccaccgg taaaattgta gcgctactcc aaaagtatgg
     8401 gttaaaggtt cagttcgaag aacgaattgg taatccgcct ttcagactta ttggaaagaa
     8461 tttgaagggg aaaatacttc gggttgaagg atcaataaat agcaagatga ttgaagcacg
     8521 attgcttatc gacccaagtt taaattccga agaaattatt gaactaaaaa actttatcat
     8581 ttcccagggt tactccacca tgacccttcg agcgcttcag ttcatgggcg taaatataga
     8641 ttggcgtgag gatgaggtgc ttattgaaca tcagatcttt gatggtagtg aactttccat
     8701 tgaagctgat tggtcgttag catcgtactg gtacgaggca gtagcccttg ccgataaagg
     8761 ggagattgct atcaaaggtc tgaaacccga aagttttcag tgcgatactg ctgtaaaaga
     8821 cttattcaca cggttaggtg taaccacaat tattgcagag aatgcaataa ttcttaaacg
     8881 caaaggaagg gtaacaaagc aatttgaaca taattttaag caacaccccg atttagcccc
     8941 tgccgcgatg tttacctgcc ttgcactagg cattcctttt acaattagtg gaattgatta
     9001 cttccatacc aaagaccccg acaggctcaa tgccattaaa gccgaattcc agaaacttgg
     9061 tgcaacaatt aatgttcaac tggaaaatga tgttgaagtt ctgaagtttg atggaaaatc
     9121 caaactaaaa aacttgaaag ttctagagtg cgattcacaa aacgatcacc gtgtagccat
     9181 gtcgctagcc ccctacgcaa ttcttggcaa gagagtggtt atgaacaatg ctcgtataac
     9241 cagtaaatca tacccaactt tttgggatga gattaaaaag ttaggttttg aggtaacagt
     9301 tttgcagtaa aaagaaaatt cttaaacagc aaaagggtga catctctgcc acccttttct
     9361 atttttggta taatctactt tttccaaact aatgtaccag caagcatatc gtgaagtgct
     9421 tgtttctttt gggtaaaacc tgctaaaata taccctatca tgaaaatcaa gctggaaaga
     9481 atttttccga aatagcgtcc ggtagctcgg gcaaacgata tacgatttcc ttccatatca
     9541 gttacaacaa ggccaatagc aatcttacca accgttcctt ggaactttga cgactccatt
     9601 atggcaaagt ataaccacat tagtacaatc aacgccaaat aggtaatcat ggctgtacca
     9661 acaattgcgc caaccatacc aatggcttca gtctcagtaa tcccatttga cgcagccgag
     9721 gccgctgaga atccaattaa tgcaagaaca ggtataacaa taatccactg aattacacca
     9781 ataattattg catcaagaat gtacgccaga aagcgccacc aaaaacctgc atactttaag
     9841 ttttccataa ttcgtaaggt ttaagtttag tttatggtta tttaaatcaa cagcaatata
     9901 ataaattcca tgtagtttca aaacaatcaa acctaattat ctacaaattc ttgccaaatt
     9961 gctatttctg gaaccaatgt tgtttagtta aggttatttg tcatgtttcg cttgcgcgaa
    10021 acatgacgct gtcgtgctga gtccggattt atagggatag acttcgctaa aacatctcaa
    10081 cgatggatga atagagatcc ttcgctgcgc tcaagatgac aagcggagtt gacagttgat
    10141 ggttgatagt tgttagtgat aaaaaatgca tttcatgaaa cacttaaaac caaacactac
    10201 tctccatttt ccctttaaac tgcacactgt acactgcaca ctattcaaaa gatccttcgc
    10261 tgcactcagg atgacaaatg aaactattcg tataagttgt tcgttattag tgggatacga
    10321 cctcacccca gcccttttcc ctgagaggga gtggagcgga agaagcaggc agaggtataa
    10381 aagcctgacc cgacaatggg tcgggggtgg aacgtggcgg tgggcctagt aagcgatacc
    10441 a
//
LOCUS       DAOOWJ010000093        10210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6561_length_10210_cov_6.426292, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000093 DAOOWJ010000000
VERSION     DAOOWJ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6561_length_10210_cov_6.426292"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1088)
                     /locus_tag="PLW22_09015"
     CDS             complement(<1..>1088)
                     /locus_tag="PLW22_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU74883.1"
                     /translation="KGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAE
                     LYKPFVIRKLIERGIVKTVKSAKKIVDRRDPVVWDILENVMKGHPVLLNRAPTLHRLG
                     IQAFQPRLIEGKAIQLHPLSCTAFNADFDGDQMAVHLPLGNEAILEAQILMLGSHNIL
                     NPANGAPITVPSQDMVLGLYYITKPRKGAKGEGTIFYSPEEVIIAYNEKAVDLHAIIK
                     VRLNTLDENGQPKQQIIETTVGRVIFNQYVPAEMGFINELLTKKSLRDISGKVLKKTS
                     VSKTAQFLDDIKDLGYYMAFKGGLSFNIDDVIIPDEKAELVEEGYAQVDEVMSNYNMG
                     LITNNERYNQIIDIWTHTNAKLTLMLMK"
     assembly_gap    1089..1188
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1189..1655)
                     /locus_tag="PLW22_09020"
     CDS             complement(<1189..1655)
                     /locus_tag="PLW22_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU74884.1"
                     /translation="MSFRRENKVKSNFSKITISLASPEEILERSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIS
                     LVVPVAHIWYFRSQPNKIGYLLGLPAKKLDAIIYYERYVVVNPGIKAADGINQ"
     gene            complement(1723..5550)
                     /gene="rpoB"
                     /locus_tag="PLW22_09025"
     CDS             complement(1723..5550)
                     /gene="rpoB"
                     /locus_tag="PLW22_09025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOU74885.1"
                     /translation="MSQNKTNNARINFSKTKSLYEYPDFLDIQLKSFMEFFKLGSTPE
                     ERKNEGLFKVFQENFPITDTRNNFVLEFLDYYVDPPRYSIEECLDRGLTYSVPLKAKL
                     KLYCTDPEHEDFDTVVQDVYLGTIPYMTPRGSFVINGAERVVVSQLHRSPGVFFGQSV
                     HPNGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFETDKDIL
                     QIFDLAEEVKVSKATLTKVVGQRLAARVLKSWIEDFVDEDTGEVVSIERNEVILDRET
                     VIEKDHIDLIIDSGVKSILVHKESQSISDYAIIYNTLQKDPSNSEKEAVVHIYRQLRN
                     SEPPDEATARDVIDKLFFSDKRYDLGDVGRYRINKKLNLNTSSDIKVLTKEDIIEIIK
                     YLIELVNSKAEVDDIDHLSNRRVRTVGEQMAAQFGVGLARMARTIRERMNVRDNEVFT
                     PIDLINSKTLSSVINSFFGTNQLSQFMDQTNPLAEMTHKRRLSALGPGGLTRERAGFE
                     VRDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINDLGFIETPYRKVENGKVGLTSN
                     DIVYLSAEEEEAKIIAQANAPLTDDGTFVNNRVKARYEGDFPLSESESVDLMDVAPNQ
                     IASIAASLIPFLEHDDANRALMGSNMMRQAVPLLQPETPIVGTGLEGQVVKDSRTQIV
                     AEENGVVEYVDADEIVVRYDKTEEELFVSFDPEIKRYKLPKFKKTNQNTSITLKPIVT
                     KGQRIVKGQVLTEGYATRDGELALGRNLKVAFMPWKGYNFEDAIVISERLLRDDVFTS
                     IHVDEYILEVRDTKRGMEELTADIPNVSEEATKNLDENGMIRIGANVVPGDILIGKIT
                     PKGESDPTPEEKLLRAIFGDKAGDVKDASLKASPSLYGVVIDKMLFSRSIKDGKEAKK
                     AKTNEKALLEKLDNEFNKQVAELKNRLVDKLFSLVNGKTSQGVYDNFDQEIVARGVKF
                     TQKILSDIDYMNVNPNKWTTDKAKNDLIKAVIQNYILKWKEYDAEFKRKKFNATIGDE
                     LPAGIMQLAKVYIAKKRKITVGDKMAGRHGNKGIVAKIVRDEDMPFLEDGTPVDIVLN
                     PLGVPSRMNLGQIYETVLGWAGQKLGIKFSTPIFDGATLEDITKYTDMAGLPRFGRTY
                     LYDGGTGERFDQPATVGVIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQR
                     FGEMEVWALEAFGASHILQEILTIKSDDVVGRAKAYESIVKGEPMPEPGIPESLNVLL
                     HELRGLGLSVNLD"
     gene            complement(5650..6030)
                     /gene="rplL"
                     /locus_tag="PLW22_09030"
     CDS             complement(5650..6030)
                     /gene="rplL"
                     /locus_tag="PLW22_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOU74886.1"
                     /translation="MADLRKFAEDLVNLSVKEVNELAKILKEEYGIEPAAAAVAVAAA
                     PAAGGEGAAAAEKTQFDVILKSAGGAKLQVVKLVKELTGLGLKEAKDLVDAAPKAVKE
                     GVSKEEAESLKAQLEEAGAEVELK"
     gene            complement(6066..6593)
                     /gene="rplJ"
                     /locus_tag="PLW22_09035"
     CDS             complement(6066..6593)
                     /gene="rplJ"
                     /locus_tag="PLW22_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOU74887.1"
                     /translation="MRREDKNALIDKLTEQINQYPHFYLTDTSELNAEVTHKLRKTCF
                     EKKVKLVVVKNTLLKKALERSGKSTDFSPIFEALKGSTSVMFTETGNVPAKLIKDFRK
                     AHPKPLFKAAYVEESIYVGESQLDVLATLKSKNELIADVIALLQSPAKNVIGSLQSGG
                     QKLSGVIKTLSEREK"
     gene            complement(6620..7321)
                     /gene="rplA"
                     /locus_tag="PLW22_09040"
     CDS             complement(6620..7321)
                     /gene="rplA"
                     /locus_tag="PLW22_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOU74888.1"
                     /translation="MTKLTKNRKLALSKVEPGKTYKLSEAAALLKEITYTKFDASVDI
                     DIRLGVDPRKANQMVRGVVTLPHGTGKNVRVLVLCTPDKEEAARQAGADYVGLDDYIE
                     KIKGGWTDVDVIITMPSVMAKLGPLGRILGPRGLMPNPKSGTVTMEVEKAVKEVKQGK
                     IDFKVDKFGIVHSSIGKISFEPDKIVDNALEFINTIIKLKPTSAKGTYIKSVYLSTTM
                     SPGIAIDTKSLTASN"
     gene            complement(7342..7782)
                     /gene="rplK"
                     /locus_tag="PLW22_09045"
     CDS             complement(7342..7782)
                     /gene="rplK"
                     /locus_tag="PLW22_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOU74889.1"
                     /translation="MAKEVAGFIKLQIKGGAANPSPPIGPALGSKGVNIMEFCKQFNA
                     RTQAQAGKVLPVVITYYTDKSFDFVVKTPPVSVTLLEITKQQKGSAEPNRKKVASVTW
                     EQVRQIAQEKMQDLNCFTIESAMKMVAGTARSMGITVTGEFPGN"
     gene            complement(7804..8352)
                     /gene="nusG"
                     /locus_tag="PLW22_09050"
     CDS             complement(7804..8352)
                     /gene="nusG"
                     /locus_tag="PLW22_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOU74890.1"
                     /translation="MEENVKKWYVLRAIGGKEKKVKELVENEIKRLKLDDFISQVLIP
                     TEKVYQIRNGKKISKERIYYPGYVLIEAALVGEIPHILRNIPNVLGFLGDPKTGEPIP
                     LRQAEVNRILGKVDELVDSEEELNVPFYVGETVKVIDGPFNSFSGVIEEINEEKKKLK
                     VMVKIFGRKTPLELSFMQVEKE"
     gene            complement(8381..8572)
                     /gene="secE"
                     /locus_tag="PLW22_09055"
     CDS             complement(8381..8572)
                     /gene="secE"
                     /locus_tag="PLW22_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOU74891.1"
                     /translation="MKITQYLHDVYTEMVQKVSWPTWKELQSSALIVMIASLLIALVV
                     FVMDFSFERLMEFVYRVLS"
     gene            complement(8592..8664)
                     /locus_tag="PLW22_09060"
     tRNA            complement(8592..8664)
                     /locus_tag="PLW22_09060"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8629..8631),aa:Trp,seq:cca)
     gene            complement(8709..9899)
                     /gene="tuf"
                     /locus_tag="PLW22_09065"
     CDS             complement(8709..9899)
                     /gene="tuf"
                     /locus_tag="PLW22_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOU74892.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKILAEKGLSEF
                     RSFDSIDNAPEEKERGITINTAHVEYQTENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVNVPRIVVFLNKCDMVDDAEMLDLVEMEVRELLSF
                     YQFDGDNVPVIRGSALGALNGEAQWVEKVMELMKAVDEYIPIPPRDNEKPFLMPVEDV
                     FSITGRGTVATGRIETGVIHTGDEVEIVGLTNDKPMKSVVTGVEMFRKILDQGEVGDN
                     VGLLLRGIDKKDIKRGMVIAKPGSITPHTKFKAEIYVLKKEEGGRHTPFHNHYRPQFY
                     LRTLDVTGEIMLPEGVEMVMPGDNVTITVELIYPVAINKNLRFAIREGGRTVGAGQVI
                     DIIE"
     gene            complement(9966..10037)
                     /locus_tag="PLW22_09070"
     tRNA            complement(9966..10037)
                     /locus_tag="PLW22_09070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10003..10005),aa:Thr,seq:ggt)
     gene            complement(10048..10120)
                     /locus_tag="PLW22_09075"
     tRNA            complement(10048..10120)
                     /locus_tag="PLW22_09075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10085..10087),aa:Gly,seq:tcc)
BASE COUNT         2654 a         2387 c         2171 g         2898 t
ORIGIN      
        1 tttttcatta gcatcaaggt caacttggcg ttggtatgtg tccagatgtc gataatctgg
       61 ttgtaacgct cgttgttggt tatgagaccc atgttgtagt tggacataac ctcgtcaacc
      121 tgtgcgtatc cttcttcaac caattcggct ttctcatcgg gaatgataac atcgtcgatg
      181 ttgaatgaca gacctccctt gaatgccatg tagtatccca agtccttgat atcatcgagg
      241 aattgagcgg ttttggacac gctggtcttc ttaagaacct taccactgat atcgcgaagc
      301 gatttcttgg tcagtaactc attaatgaag cccatctcag caggaacgta ttggttaaat
      361 attacgcgtc ctactgtagt ttcaattatt tgttgcttag gttgaccgtt ttcatctaag
      421 gtatttaacc taaccttgat aatagcatgt aggtcaacgg ctttctcatt gtaagcaatg
      481 ataacctcct cgggtgagta gaaaattgtt ccctcgccct tggcaccctt acggggtttt
      541 gttatgtagt atagtcccag taccatgtcc tgcgaaggta cagtgatagg agcaccgttt
      601 gcagggttca aaatgttgtg ggaacctaac atgagtatct gggcctccag aatagcctcg
      661 tttccaaggg gtaagtgtac agccatttgg tcaccgtcaa agtcggcgtt aaacgcagta
      721 cacgacagtg ggtggagctg aatggcctta ccctcaataa gacggggctg gaaagcctga
      781 atacctagcc tgtgaagggt tggagcacgg ttcagtagaa cgggatggcc cttcatcacg
      841 ttctcaagga tatcccatac aactggatcg cgcctgtcaa ctatcttttt ggccgatttt
      901 acggttttaa caatacctct ctcaataagc ttacggatta caaaaggctt gtaaagctct
      961 gcagccatat cctttggcaa tccgcactcg tgcatcttta actctgggcc aacaacaata
     1021 accgaacgtg cagaatagtc gacacgttta cccaagaggt tttggcggaa acgtccctgc
     1081 ttacccttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ctgattaata
     1201 ccatcggcag ccttaatgcc tggattaaca accacgtaac gctcgtagta tataatggca
     1261 tcgagttttt tagcaggtag tccaagcagg tagccaatct tgttgggttg tgaacggaaa
     1321 taccagatat gagcaacagg aacaacaagg ctgatatgtc ccatgcgttc acggcgtacc
     1381 ttcttctcgg taacctctac cccacatcgg tcgcaaacta tccccttgta cctgatacgc
     1441 ttgtactttc cgcagtggca ctcgtagtcc ttcacaggac caaaaatgcg ttcgcagaac
     1501 aaaccgtcgc gttcgggttt gtatgttcta tagttaatgg tttcaggttt aagcacctca
     1561 ccgcttgaac gctccaaaat ctcctcgggg gatgctaagc taatggtgat ttttgaaaag
     1621 ttgcttttaa ccttattctc ccttctgaac gacatattat tcgtgttata agtttgttat
     1681 gccaatccgg aaagggcctt tgccctttcc acggcatatt gattaatcca gatttacact
     1741 caaccctaaa ccacggagct catgaagcaa tacgttaagc gattcaggaa tgcccggttc
     1801 aggcattggt tctcccttaa ctatcgactc gtatgcttta gctcttccta ctacatcgtc
     1861 ggacttaatg gtaaggattt cctgaaggat gtgcgaagcg ccaaatgctt ccagtgccca
     1921 cacctccatt tcaccaaaac gctgaccacc aaactgagcc ttaccaccaa ggggttgctg
     1981 agtaatgagt gagtaaggtc caatggaacg ggcatgcatc ttatcgtcaa ccatgtgacc
     2041 gagcttaagc atgtaaataa cccctacagt agcaggttgg tcgaaccttt cacctgttcc
     2101 gccatcgtaa aggtatgttc taccaaaacg gggtagccca gccatatcgg tgtacttggt
     2161 aatatcttca agagtagcac catcaaaaat gggtgtacta aacttaatgc ccagcttttg
     2221 acctgcccat ccaagtacgg tttcgtaaat ctgaccgagg ttcatacgtg atggcacgcc
     2281 aagggggtta agcactatgt ccacaggagt accatcctct aggaatggca tatcctcatc
     2341 gcgaacaatt tttgctacaa tacccttgtt accgtgtcga ccagccattt tatcacctac
     2401 ggtgatttta cgtttcttgg cgatgtaaac cttggccagc tgcattattc cagcaggaag
     2461 ctcatcgcca attgttgcgt tgaacttctt acgcttgaat tcagcatcgt actccttcca
     2521 cttaaggatg taattctgta ttactgcttt aatcaggtcg ttcttagcct tatcggtagt
     2581 ccacttgttg gggtttacgt tcatgtaatc aatgtccgac aggatttttt gggtgaactt
     2641 aacaccacga gcaactattt cctgatcgaa attatcgtaa acaccctggc tggtcttacc
     2701 gttaactagt gagaaaagct tatcaactaa acggttctta agttcagcaa cctgtttatt
     2761 aaactcattg tcgagtttct caagcagagc cttctcatta gtcttggcct ttttagcctc
     2821 cttaccatcc ttaatggaac gtgagaataa catcttatca ataaccaccc cgtaaagcga
     2881 aggtgaagcc tttagtgagg catccttaac atcaccagcc ttatcgccga agatagccct
     2941 taagagtttc tcttcagggg ttgggtccga ttctccttta ggagtaattt taccaataag
     3001 gatatcaccc ggaacaacat tagcgccaat tctaatcata ccgttttcgt cgaggttttt
     3061 tgtagcctct tcggaaacgt taggaatatc ggcggtaagc tcttccatac ctcgcttggt
     3121 atcgcgaacc tcaaggatgt actcatcaac atgaattgat gtaaacacat cgtcgcggag
     3181 taaacgctca ctgattacaa tagcatcctc aaagttgtag cctttccaag gcatgaatgc
     3241 aaccttaagg tttctaccca aggccaactc tccatctctg gttgcatacc cttcggtcag
     3301 aacttgtcct tttacaatac gttgtccctt tgtaacaatg ggcttaagcg taatggaggt
     3361 gttctggttg gtttttttga acttaggtag cttgtagcgt ttaatttcgg gatcgaaact
     3421 tacaaagagt tcctcttcgg ttttatcgta acgaacaact atttcgtcgg catcaacata
     3481 ctcaacaaca ccattctcct cagctacaat ctgagttcgg ctatccttaa caacctgacc
     3541 ctctaagcct gtaccaacaa ttggggtttc gggctggaga aggggtacag cttgtcgcat
     3601 catgttcgat cccatgagcg cacggttagc gtcgtcgtgc tcaaggaacg gaattagcga
     3661 tgcagctatt gacgcaatct ggtttggagc aacgtccatt aggtcaaccg attcactttc
     3721 ggataacggg aaatcgccct cgtagcgagc tttcaccctg ttgtttacaa aagtaccgtc
     3781 atcggtaagg ggagcgtttg cctgtgcaat aatttttgct tcctcttcct cggcgctcag
     3841 gtaaacaata tcgttactag tcaggccaac tttaccattc tcaaccttgc ggtaaggggt
     3901 ttcaataaac ccaaggtcgt tgatctttgc ataaacgcaa agggatgaga taagaccaat
     3961 gttaggccct tcaggggttt caatggggca aaggcgtccg taatgtgtat agtgaacgtc
     4021 gcgaacctcg aaaccagctc gttcgcgagt aagaccacca ggtcccaaag ccgataaacg
     4081 acgcttgtgg gtcatttcgg ccagcgggtt ggtctgatcc atgaactgcg aaagctggtt
     4141 tgttccgaag aacgagttga taaccgacga gagtgttttg gagttaatca gatcgatagg
     4201 agtaaacacc tcgttatcgc gaacgttcat acgctcacgg atggttctgg ccatgcgggc
     4261 taaaccaacg ccaaactgag cagccatctg ttcacccacg gttcgcaccc tacggttact
     4321 taggtggtca atatcatcaa cctcagcctt tgagttaacc agctcaatga gatacttaat
     4381 aatctcaatg atatcttcct tggttaacac cttaatgtcg gacgaggtgt tcaggttaag
     4441 ctttttgttg atacggtaac gtcctacatc acccaagtcg taacgtttat cggagaagaa
     4501 tagcttatcg ataacgtcgc gggcggtagc ctcatcgggt ggttcggagt tacggagctg
     4561 cctgtaaatg tgtaccacag cctccttctc agagttactg gggtcttttt gcagcgtgtt
     4621 atagattatg gcataatcgg aaatgctctg tgattcttta tgaacaagaa ttgacttaac
     4681 accggagtcg ataattaaat cgatgtggtc cttttcaatg acggtttcgc ggtcaaggat
     4741 aacctcgtta cgctcaattg aaaccacctc accggtatct tcatcaacaa agtcctcaat
     4801 ccaactcttg agaacgcgag cggccaaacg ttgccccact accttggtga gagtggcttt
     4861 tgaaaccttt acctcttctg ctaagtcgaa tatctgcagg atatccttat cggtctcaaa
     4921 tccaatggcg cgcaaaagtg tagtaacggg caatttcttt ttacgatcga tgtatgcata
     4981 catcacgtta ttgatgtcgg tggcaaactc aatccacgat cccttgaagg ggataatacg
     5041 tgcggagtag agcttggtac cgttaggatg tacgctttgg ccaaagaaca caccgggcga
     5101 gcgatgaagc tgcgaaacaa ctactcgttc agctccatta attacaaaac taccgcgggg
     5161 tgtcatgtag ggaattgttc ccaggtatac atcctgaact accgtatcaa aatcctcatg
     5221 ttcgggatcg gtacagtata gctttaactt tgcctttagt ggtacgctgt aggttaaccc
     5281 tctatccaga cattcctcaa tggaatagcg gggaggatca acgtagtaat cgagaaattc
     5341 gagtacaaaa ttgttcctgg tatcggtaat tggaaaattc tcctgaaaca ctttgaacag
     5401 gccttcgttc ttacgctctt cgggagttga acccagcttg aagaactcca taaacgactt
     5461 tagctgaata tccagaaaat cgggatactc gtataggctc tttgttttgg agaaattgat
     5521 acgtgcgttg ttagttttat tttgagacat tgcgaacgaa ttggttgaac tataaaataa
     5581 acataacaag aaaaaggctt agaatctatc gggatagatt ctaaacctta gaaccactat
     5641 aggttaggac tatttaagtt caacttctgc tcctgcctct tcaagttgtg cttttaatga
     5701 ttcagcttct tctttagaaa caccttcctt aacggcctta ggagcagcat caactagatc
     5761 cttagcttcc tttagaccaa gaccggtaag ttcctttaca agcttaacaa cctgcaattt
     5821 agcaccgcca gctgacttaa gaataacgtc aaactgagtt ttttcagcgg cagcagcacc
     5881 ttcgccacca gcagcgggag cagcagcaac agctacagct gcagcagcag gttcaatacc
     5941 atactcttcc ttgagtattt tagccaattc gttaacttcc tttactgaga ggttaaccaa
     6001 atcttctgca aattttctaa gatctgccat tttagtaagt gtttaatttg ttctcaaaat
     6061 aatttttact tttccctttc agagagtgtt ttgataacac ccgataattt ttgaccacca
     6121 gattgtagcg aaccgataac gttctttgcg ggcgactgga gtagtgcaat aacatcggca
     6181 ataagctcgt tcttgctctt cagggttgcc agcacatcaa gttgactctc gccaacatat
     6241 atcgattctt caacataggc agccttaaat agaggctttg gatgagcctt gcgaaaatcc
     6301 ttgataagct tagccggaac attacctgtt tcagtaaaca ttactgaggt tgagcctttc
     6361 aatgcttcaa agattggcga gaaatcagtt gatttgccgc ttctctcaag ggctttcttc
     6421 agcaatgtgt tcttaaccac taccagcttt acctttttct caaagcaggt cttacgtaac
     6481 ttgtgagtta cctctgcgtt aagttccgag gtatcggtaa ggtaaaaatg ggggtactgg
     6541 ttaatttgct cggtcagctt atcaatcagt gcgtttttat cttcccttct cataacaaat
     6601 tgggtctgtg taaacgttat taattcgaag ccgtgagcga cttggtgtca atagcaatac
     6661 cggggctcat ggtagtggaa aggtatacac tcttaatgta ggtaccttta gccgacgtag
     6721 gtttgagctt aatgatggta ttgataaact caagcgcatt atcaacaatc ttgtcaggct
     6781 cgaatgaaat ttttccgatt gatgagtgga ctataccaaa cttgtcaacc ttaaagtcaa
     6841 tcttaccctg ctttacctcc ttaacagcct tttcaacttc catggtaacc gtaccactct
     6901 tggggtttgg catcaaaccg cgggggccaa gaatacgtcc taaaggacct agcttagcca
     6961 taaccgaggg catggtaata ataacatcca catcggtcca tccgcccttg atcttctcga
     7021 tgtaatcgtc aaggcctacg tagtcggcgc cagcctggcg agcagcctcc tccttatcgg
     7081 gagtacagag taccagaacg cgaacgttct taccggtacc atgcggaagg gttaccacac
     7141 cacgaaccat ctggttggcc ttacgggggt caacccctag gcgaatatca atatcaaccg
     7201 atgcatcgaa cttggtgtat gtaatttcct tcaacagtgc ggctgcttcc gatagcttgt
     7261 aagtcttacc gggctctact ttcgacagag caagcttcct gttttttgtc agtttagtca
     7321 ttgcgcaaat gaggttaaat gttaattccc cggaaactct ccggtaacag tgatacccat
     7381 gctgcgggct gtacctgcca ccatcttcat tgctgattca atggtaaaac agttcagatc
     7441 ctgcatcttt tcctgggcaa tttgtcgaac ttgctcccag gttacagagg caaccttttt
     7501 cctgttaggc tcagccgatc ccttttgttg cttggttatc tcaagcaaag ttacggatac
     7561 cggtggggtt tttacgacaa aatcgaacga tttgtcggta taatatgtaa taaccacagg
     7621 tagcactttg cctgcctgtg cttgagttct ggcgttgaac tgcttgcaga actccataat
     7681 atttaccccc ttggagccaa gagcaggacc aattggtggt gaagggtttg cagcaccacc
     7741 cttaatctgg agtttaataa atccagctac ttctttagcc atagtgcgtt tgctctttaa
     7801 ttattactcc ttttcaactt gcataaaact taactccagt ggagttttac ggccgaagat
     7861 tttcaccata accttcagct tcttcttctc ttcgttaatc tcttcgatta ctccagaaaa
     7921 agaattgaat ggtccatcaa ttactttaac tgtttcacca acgtagaacg gaacattcaa
     7981 ctcttcctcg ctgtccacca attcatctac cttgccaagt attctattga cttcggcttg
     8041 acgaagtgga atcggttctc cggtttttgg gtcacctaaa aaaccaagga cattggggat
     8101 gtttcgtaag atgtgaggaa tttcacctac cagtgcagct tcaatcagaa cgtagccagg
     8161 gtagtagatt ctttccttgc tgattttttt gccatttcgg atctggtaaa ctttctcagt
     8221 aggaataaga acctgagaga taaagtcgtc gagtttcaaa cgcttgattt cgttttccac
     8281 caactccttt accttcttct ccttaccacc aatggcccta agaacatacc attttttaac
     8341 attctcttcc attacagctc cttcttaggc cgttctattg ttagctaagt acacggtaaa
     8401 cgaactccat caatctttcg aaagagaagt ccataacgaa gacaaccagt gcgattaaaa
     8461 gggaagcaat cataacaatt aatgcactgg actgaagttc tttccatgtt ggccacgaaa
     8521 ctttttgcac catctcggtg taaacgtcgt gcaggtactg tgtaattttc atgtcaacag
     8581 tttgtgttta gcacgggtgg agagactcga actcccagcc aatggttttg gagaccacta
     8641 ctctaccaat tgagctacac ccgtataaga cccccacggt tcaaatgaac cgttggggta
     8701 ctattgtatt actcaataat gtcaattacc tgaccagcac caacagtcct accaccttcg
     8761 cggatagcaa agcgtaggtt cttgttgata gcaacagggt agataagttc tacagtgata
     8821 gttacgttat cgccaggcat taccatttca acgccttcgg gaagcataat ttcaccggtt
     8881 acgtcgaggg tacgtaggta gaactgagga cggtagtggt tgtggaaagg agtgtgacgg
     8941 ccaccctctt ctttcttcaa cacgtaaatc tcagctttga acttggtgtg aggggtaatt
     9001 gagcctggct tagcaataac cataccacgc ttaatatcct tcttgtcaat accgcggagt
     9061 agtagaccta cgttgtcgcc aacttcaccc tgatcaagga tcttacggaa catttcaaca
     9121 ccggtaacta ctgacttcat aggtttgtcg ttggttaaac ctacgatttc aacttcgtcg
     9181 ccagtgtgga taacgccagt ttcaattcta ccggtagcaa cagtaccacg accagtgatt
     9241 gagaatacgt cctcaacagg cattaggaat ggcttttcgt tgtcacgtgg aggaatggga
     9301 atgtactcat caacagcttt catgagttcc attacctttt caacccactg agcctcaccg
     9361 tttagggcac caagagcaga accacggata acgggtacgt tatcgccatc gaactgatag
     9421 aagctcaaga gctcacgtac ttccatttca acgaggtcaa gcatttcagc atcgtcaacc
     9481 atgtcgcact tgttcaggaa cacaacgatc ctaggaacgt ttacctgacg agcgagaagg
     9541 atgtgctcac gggtttgagg cataggacca tcagtagcag ctacaacaag gatagcgccg
     9601 tccatctggg cagcaccagt aatcatgttc ttaacatagt cagcgtgacc aggacagtca
     9661 acgtgagcgt agtgacggtt ctcagtttga tactcaacgt gagcggtgtt aatggtaata
     9721 ccgcgctctt tttcttcagg agcgttgtcg atagagtcga atgaacggaa ttcggaaaga
     9781 cccttttcag ctaatatttt agtaatagca gcggtaaggg tagtcttacc atggtcaacg
     9841 tggccaatgg taccgatgtt cacgtgcggt ttcgacctat caaatttttc tttagccata
     9901 gttttctggt attaatttaa aattagtatt aaaaacttga ttgtctcata caacacaaaa
     9961 aattgagccg atgacgagaa ttgaactcgt gaccccttcc ttaccaagga agtgctctac
    10021 ctctgagcta catcggctta caggtagagc gggagacgag actcgaaccc gcgaccctca
    10081 gcttggaagg ctgatgctct accgactgag ctactcccgc atgccaccaa ccctgcagga
    10141 ctggtgtcaa ccctgttttg tggggagagc aggattcgaa cctacgaagg cataagccag
    10201 cagatttaca
//
LOCUS       DAOOWJ010000094        10097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6664_length_10097_cov_8.819558, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000094 DAOOWJ010000000
VERSION     DAOOWJ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6664_length_10097_cov_8.819558"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            637..1038
                     /locus_tag="PLW22_09080"
     CDS             637..1038
                     /locus_tag="PLW22_09080"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOU74893.1"
                     /translation="MKGTVSTNQKVELEYQVNTSPGVLFKRLSTPHGLSEWFANDVNL
                     SGNVFTFIWDGDTKKAELVDKKENKFVKFRWITEQNEAGHHFAFKLTKDELTGEVSLT
                     VIEEVNEEEDIDEVVSLWNYQIGELKRLIGA"
     gene            1110..2588
                     /locus_tag="PLW22_09085"
     CDS             1110..2588
                     /locus_tag="PLW22_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU74894.1"
                     /translation="MKTLHRFILKSYIGPLVMTFFITMFVLLMQFLWKYIDDLVGKGL
                     SWNVIAELLLYASAGLLPMALPLATLLASLMTMGNLGENNELLAMKSAGISLPRILTP
                     LIVLTIFISVGAFFFSNNVLPYTNLKISTLLYSVKQQKPELIIKEGVFTNLIEGYSIK
                     VGDKDPNTGLMRRILIYDHSKDEENTTVTYADSGYMKPTADQKFLVATLYSGNSYKEV
                     RDNRKPRYSYKEKLPAQKQTFEKEVIIFELKGFELQRADESFFKDSYQTQNIAQLEKT
                     EDSLSKAFNTQIESFSQHVIRSSIFRNPYWGRRQNDAKVTRSLNVDSLYNTLPPVEKI
                     SALERAMEFARSTKSYVSSSKDEFFYKQKYIARHMIEWHRKFTLAFACFVFFFIGAPL
                     GAIIRKGGLGTPVVISVLFFIIYYIINITGEKFARELYWNPFMGMWISSFILLPLGIF
                     LSYKATTDSVIMNADFYIEKVKKLFSFSTTRKKHTELSPKTE"
     gene            2596..3804
                     /locus_tag="PLW22_09090"
     CDS             2596..3804
                     /locus_tag="PLW22_09090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU74895.1"
                     /translation="MRILIISHKPPYPSVDGGTLATLNMCLGLAKAGNNVTVLTLSTQ
                     KHPSSLEIIPNEIKALINFEILHVNIKTTLLGYILNFIFSLRPYNIERFISTPFKRLI
                     RKILLNEYFDVVQLEGLYLYPYISTIRSHFRGTLSLRSHNVEHHIWLKLAENETNKLK
                     RFYFRILAKRLARVESNISSKVDALVAIAEPDRQWFVENNFNKPTITIPAGYLKEEVA
                     EDIEPVDFPSICYIGALDWLPNTEGLNWFINWVWPRIQSEIPEIEFHIAGRNASEELA
                     ERLITERNIIFHGQVANSSTFLSRCPIMVVPLLSGSGIRIKIIEGMYLQRAIVATSMA
                     VKGIDIKHNEHILIADSPDEFAKAVCSLIYNPDLGKKIAHNAMEFAQNNLDAIVLAEK
                     LTQFYKQLEG"
     gene            3801..>4039
                     /locus_tag="PLW22_09095"
     CDS             3801..>4039
                     /locus_tag="PLW22_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU74896.1"
                     /translation="MMLHFLFWLTIGIVLYTYCIYLLVLWILCVKREKNNSPENNISQ
                     LPEVTIVIAAHNEEKYILAKYQNTLALNYPSGKLF"
     assembly_gap    4040..4139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4140..4667
                     /locus_tag="PLW22_09100"
     CDS             <4140..4667
                     /locus_tag="PLW22_09100"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU74897.1"
                     /translation="ILDDFEISASIIKQGYKVKYENGAVASETGSLTVEDEKKRKVRI
                     AAGCFQALGRHAYLLNPFKNTEVAFKFFSHKLLRWVLVPPAIVGAPFLNAAILLLYPD
                     SSIIYLVSMLTITFFYLLVAIGYLFRNRSNVHPVVTFPYYGFMMNITMLKGLIIFLTG
                     KQTHIWEKANRKADI"
     gene            4735..5970
                     /locus_tag="PLW22_09105"
     CDS             4735..5970
                     /locus_tag="PLW22_09105"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOU74898.1"
                     /translation="MANNDEIKLNTIEEAIEELRNGRVIIVVDDEDRENEGDFIAAAE
                     LITPEIINFMAKYGRGLICASLPEKRCDELELELMVGRNTALHHTAFTVSVDLIGNGC
                     TTGISAADRAKTIKALVDPNTKPEDLARPGHIFPLRAKEKGVLRRPGHTEAAIDLTQL
                     AGLTPGGVLVEIMNDDGTMARLPQLVQVAQEFNLKIVTIKDLISYRLKFESIVEKGVK
                     VKLPTEFGDFALIPFRQKSNGLEHVALIKGEWEPDEPVLVRVHSSCVTGDIFGSYRCD
                     CGPQLHEAMKMIDNAGKGVLVYLNQEGRGIGLFNKIHAYKLQEEGMDTVDANVELGFQ
                     PDERDYGVGASILREVGVKNMRLITNNPKKMSGLEGYGLHVVENIPLVIPPNPYNEFY
                     LKTKHERMGHINLFNPKKD"
     gene            5977..6957
                     /gene="fmt"
                     /locus_tag="PLW22_09110"
     CDS             5977..6957
                     /gene="fmt"
                     /locus_tag="PLW22_09110"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOU74899.1"
                     /translation="MPAKESPRIVYMGTPEFAVEPLRAIIEAGYNVSAVVTVPDKPAG
                     RGLKLQPSPVKVFAEQNHIPVLQPEKLKDPQFIQTLKEINPDIAVVVAFRMLPRDVWQ
                     IPRLGTFNLHASLLPQYRGAAPINWAIINGEKETGITTFLLDEQIDTGNILLQKSVPI
                     GETETAGELHDRLMYQGAGLVVETICMLTAGNINPIPQNQIKISGELKPAPKIFKETC
                     RVDWNNPSQKVFNHIRGLSPYPAAWTELKTKRNNVETIIPIKIYQAEKLDQAQSQRPG
                     MLKTDGKTHLHVFCSQGAIALKTVQQAGKKAMGIEEFLRGWQNTELIEVL"
     gene            complement(6952..7887)
                     /locus_tag="PLW22_09115"
     CDS             complement(6952..7887)
                     /locus_tag="PLW22_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOU74900.1"
                     /translation="MNRILVALFFILTGIHLSVFSQNKISREEYISMYSHIAVNNMKQ
                     FGVPASITLAQAMLESDNGNSTLAVKANNHFGIKCHKDWTGATIYHDDDRKGECFRKY
                     KNPEQSFNDHSLFLRGGKRYAFLFDLTPTDYKAWAHGLKKAGYATNPKYAELLIKIIE
                     DNELYRFDQGITVAIKPPKPVVTDWDNYEIDLYKTRPVFIRNRVKYIVAKDGDTFESL
                     ARELDLMPWQLYRYNDLTRDSVIRPGQELYIQPKRWKADRSNPVHTAEEGETMYKISQ
                     MYGVKLKSLYRKNRMKPGDEPEVGQLIYLRKRKPL"
     gene            complement(7896..8594)
                     /locus_tag="PLW22_09120"
     CDS             complement(7896..8594)
                     /locus_tag="PLW22_09120"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU74901.1"
                     /translation="MINFSSDRILYEDNHFLAVNKNCGEIVQADKTGDESLVETVKRF
                     IKERDSKPGRVFLEVTHRIDRPVSGVVLFAKTSKGLSRINNLFQEGKITKKYWAIVKN
                     QPPVESQTLVHYLLRDNKKNKSFVYHREVHGSKIAKLKYELVGRTENYYLLVIDLITG
                     RHHQIRAQLSKIGCPIRGDLKYGYPRSNPDGGIDLHSRVFAFTHPISGEEIKIVAPPP
                     SEKLWSEFLLLGLE"
     gene            complement(8603..9418)
                     /gene="panB"
                     /locus_tag="PLW22_09125"
     CDS             complement(8603..9418)
                     /gene="panB"
                     /locus_tag="PLW22_09125"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOU74902.1"
                     /translation="MSSESKVKVVTTHVLQEMKVRGEKIAMLTGYDYSMARILDAAGI
                     DVLLVGDSASNVMAGHISTLPITLDQMIYHASSVVRGVKRALVVVDMPFGTYQGNSKL
                     ALRSAIRIMKESGADAVKLEGGSEIIDSVERILSAGIPVMGHLGLTPQSIHKFGTYVV
                     RAREEAEAKKLVEDAHLLERTGCFAIVLEKIPAALGKQVASELKIPVIGIGAGSGVDG
                     QVLVTHDMLGINQEFSPRFLRRYHNLYAEMLGAFQAYISDVKSLDFPNEREQY"
     gene            9727..>10097
                     /locus_tag="PLW22_09130"
     CDS             9727..>10097
                     /locus_tag="PLW22_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOU74903.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGNEPVVITNSEGKRTTPSVVAFAE
                     NGERKVGDPAKRQAIINPQKTIFSIKRFMGETYDKVAQVEVPRVPYKVVRGENNTPRV
                     DIDGRLYTPQEISSMILQKMK"
BASE COUNT         3000 a         2196 c         2032 g         2769 t
ORIGIN      
        1 aggtcgccgg ttcaatcccg gctctcgcta caaagtcgaa aagcccgtaa acagtgagtt
       61 tgcgggcttt tttattttca acctttacca ataataaccc agttccccct ctgggtagct
      121 cagttggtta gagcgtcccg atttcatcgg gaaggtcgcc ggttcaatcc cggctctctc
      181 tacaaagccg aaaagcccgt aaacagtgag tttgcgggct tttttgtttt taacctttac
      241 caatagtaac ctagttcccc cctgtgggta gctcagttgg ttagagcatc ccagactcat
      301 aggaaagtcg ctggttagga atgtaaacgt tcttatttga gatattagtt agttgacgag
      361 gtagctcagc cagtcagagc gtcccgattt catcgggaag gtcgccggtt caataggagt
      421 catagaggtt ataatatcac ttactttaac atcaaggtac ttggccacaa cggattgcgc
      481 cctcttaaat aggctaacaa tttctttgtt tattaacaac ctattgttaa taaatacctt
      541 caaaacattg gcataagtat tgcattttat ttttaaattg cttatgctaa gttcaagaat
      601 tggtatgagc agagcgtaaa tttaaaacat tcaagtatga aagggacagt aagtactaat
      661 caaaaggttg aacttgagta tcaggttaac acctctcctg gtgtgctttt taaaaggtta
      721 agcacccccc atggtttatc ggaatggttt gctaacgatg taaacctttc agggaatgtg
      781 ttcactttca tctgggacgg agataccaaa aaagctgaat tggttgataa aaaggaaaat
      841 aagtttgtta agtttcgatg gattactgag cagaatgagg ctggccacca ttttgctttt
      901 aaacttacaa aagatgaact caccggcgag gtatcgctta ccgtaattga agaggttaat
      961 gaggaggagg atatcgatga ggttgtttca ctctggaact accaaattgg tgaactcaaa
     1021 cggctgattg gggcataagc taaatataac atctatcact ttcaaggaaa aacattacat
     1081 ttgtgatgtt tttccttaat ttttaatccg tgaaaacact tcatcgtttt atcctgaaat
     1141 cgtatattgg cccgctagta atgacatttt tcattactat gttcgtactg ctcatgcagt
     1201 ttctctggaa gtatatcgac gatttggttg ggaaagggct gagctggaat gttattgcag
     1261 agcttctttt atacgcatcg gcaggacttt tgcccatggc attgccactt gcaaccctac
     1321 tcgcctcgct tatgaccatg ggcaacctag gggagaataa tgagctgctt gccatgaaat
     1381 cggcaggaat ctcactaccc cggatactta ctccacttat agtacttacc atttttatta
     1441 gcgttggcgc attctttttc tcaaacaatg ttctgcccta cacaaacctt aaaatatcga
     1501 cactgctcta ctcggttaag caacaaaaac cagaacttat aataaaggaa ggggttttta
     1561 ccaacctgat tgaaggatac agtataaaag taggcgacaa agacccaaat accggattaa
     1621 tgcgccgaat ccttatttac gatcacagca aggacgaaga aaacactacc gttacctacg
     1681 ccgattccgg ttacatgaaa cccactgccg atcaaaagtt tttggtagcc acactctata
     1741 gcggtaatag ctacaaggag gttagggata atcgtaagcc ccgttacagc tataaggaaa
     1801 aactcccagc ccagaagcaa acctttgaaa aggaagtaat catatttgaa ctaaaaggtt
     1861 ttgaacttca gcgtgccgat gagagttttt tcaaggacag ctaccaaacc caaaatattg
     1921 cccagctaga aaaaacggag gattcacttt ccaaagcatt taacactcaa atagaatcat
     1981 tctcgcaaca tgtaatcagg agtagtatct tccgaaatcc atactggggc cgtcgccaaa
     2041 acgatgctaa agtcacacgt agtttgaatg ttgattccct ttacaataca ctacctcccg
     2101 tagaaaaaat ttcggcactg gagcgtgcca tggaatttgc ccgctcaacc aagagctatg
     2161 tctcatcgtc aaaggatgaa tttttctata aacaaaagta tattgccagg catatgattg
     2221 aatggcaccg aaaattcacc cttgcatttg catgctttgt attctttttc ataggcgcac
     2281 cacttggggc cataatcagg aaaggcggat tgggtacacc tgttgtgatt tcggttcttt
     2341 tcttcattat ctactacatc ataaacatta ccggcgaaaa gtttgctcgc gagctttact
     2401 ggaacccctt tatgggcatg tggatttcat cgtttattct tcttccccta gggatattcc
     2461 tctcttacaa ggcaacaacc gattcagtga tcatgaatgc agatttctac attgaaaagg
     2521 ttaaaaagct gtttagcttt agcacgacaa gaaagaagca taccgaatta tcgcctaaaa
     2581 ccgaataaat tttaaatgcg aatccttata atttcacaca aaccacccta ccccagtgtt
     2641 gacggaggaa cactggcaac ccttaatatg tgcctagggc tagccaaagc aggcaataat
     2701 gtaaccgtac taaccttaag tacccaaaag cacccaagct cactggaaat aattccaaat
     2761 gagattaaag ctcttattaa ctttgaaata cttcacgtta atattaaaac aaccctttta
     2821 ggatacatcc tcaacttcat tttttcgctt cgcccctata acattgagcg attcataagt
     2881 acccccttta aaaggttaat aagaaaaatt cttctgaacg agtattttga tgttgttcag
     2941 cttgagggtt tataccttta cccatacatt agtaccattc gcagtcattt taggggaacc
     3001 ctttcgctta ggagccataa tgtggagcat catatttggc taaaacttgc cgaaaacgag
     3061 accaataagc tcaaaaggtt ctattttagg attcttgcca aacgtttggc cagagttgaa
     3121 tcgaacattt caagtaaggt tgatgccctt gtagctatag ctgagcccga caggcaatgg
     3181 tttgtggaaa ataattttaa caaaccaaca attactatcc ccgctgggta cttaaaggag
     3241 gaggttgcag aagatattga gcctgttgac ttcccatcaa tatgttacat aggcgcattg
     3301 gattggttgc caaatacaga aggtttaaat tggtttatca actgggtttg gccacgtatt
     3361 caatcggaaa ttcctgagat agagtttcat attgccggac gaaacgcctc agaggaactg
     3421 gcagaaagac ttattaccga gcgcaacatc atttttcatg gccaggtagc caattcatct
     3481 acttttctga gtagatgccc aataatggtc gtacctctac tctcaggtag tggaattcgg
     3541 attaaaatta ttgagggcat gtacctgcaa agagccattg ttgccacaag tatggctgtt
     3601 aagggtattg atatcaagca caacgagcac attctcatag ccgattcgcc cgatgaattt
     3661 gccaaagcgg tatgctcgct aatctacaac cccgacctag gaaagaaaat tgcgcataac
     3721 gccatggaat ttgctcaaaa caacctcgat gctattgtgc tagccgaaaa acttacccag
     3781 ttttacaaac aacttgaggg atgatgctcc attttctttt ctggctaaca attggtatag
     3841 ttctttacac atactgcata tacttactgg tactttggat attatgtgta aaacgggaaa
     3901 aaaacaattc acctgaaaac aatattagtc aacttccgga agtgaccatc gttattgccg
     3961 cacataacga ggaaaaatac atattagcaa aatatcaaaa caccctagcc ctgaactacc
     4021 cttccggaaa attattccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     4141 tactcgacga ctttgaaata tcggcaagta ttattaaaca gggatataag gttaaatacg
     4201 aaaatggagc agtagcttcg gaaactggct ccctcacggt agaggatgag aaaaaacgaa
     4261 aggtaagaat agctgctggg tgctttcaag cacttggtag gcatgcatat ctacttaatc
     4321 catttaaaaa taccgaagtg gcttttaagt ttttttcaca taaactttta cgatgggttt
     4381 tggtacctcc tgcaattgtt ggtgcaccgt tcttaaatgc tgccattcta ttattatatc
     4441 cagatagttc cattatctac ttagtttcca tgcttactat tacttttttt taccttttag
     4501 tagccatagg ataccttttc cggaatcgca gcaatgtgca tcccgtggtt accttcccat
     4561 actatggttt tatgatgaat attaccatgc ttaaagggtt aattattttc ttaactggca
     4621 aacaaacaca tatttgggaa aaggcaaacc gaaaagctga catttagcaa gatatttttc
     4681 ctttcaaaat actaattttg cgtcatcctt tttaatcatt ttattggcag cacaatggca
     4741 aacaacgacg agattaaact aaataccatc gaagaggcga ttgaagagct tcgtaatgga
     4801 agagtaataa tagtggttga cgatgaggat agggagaacg agggcgattt cattgctgct
     4861 gctgagctaa tcacacctga aataattaac tttatggcta agtatgggcg tgggctaatt
     4921 tgtgcatcac tgcccgaaaa gcgctgcgat gagttggagc ttgagctcat ggtaggccgt
     4981 aacaccgctc tgcaccatac agcctttacc gtatcggtcg accttattgg aaacggatgt
     5041 acaactggta tatcggcagc cgatagagcc aaaaccataa aggcactcgt tgaccccaat
     5101 actaaaccgg aagaccttgc ccgaccaggt catattttcc cgctacgtgc caaagaaaag
     5161 ggcgttttac gtcgcccagg ccataccgag gcagctattg accttacaca gcttgccggc
     5221 ctaacccccg gtggtgtatt ggttgaaata atgaacgacg atggcaccat ggcacgcctt
     5281 ccccagcttg ttcaggttgc ccaggagttc aacctgaaaa ttgtaaccat aaaggaccta
     5341 attagctata ggctgaagtt tgaaagtatt gttgagaaag gtgttaaggt aaaactccct
     5401 acggagtttg gcgattttgc actaatacct ttccgccaga agtcgaacgg gcttgagcat
     5461 gttgccctaa ttaaaggcga atgggaaccc gatgagcccg ttctggttcg cgtacactcc
     5521 tcgtgtgtta ccggcgatat ttttggctcg taccgttgcg attgcggccc tcagctccat
     5581 gaggccatga agatgattga caatgcaggt aaaggggtgc tggtttacct taaccaggaa
     5641 gggcgtggca taggcctttt caacaagata catgcatata aactccaaga ggaagggatg
     5701 gatacagttg atgccaacgt ggaacttgga ttccagcccg atgagcgcga ctatggtgtg
     5761 ggagcaagta ttttgcgcga agttggtgta aagaacatgc ggcttattac caacaaccct
     5821 aaaaagatga gtgggcttga agggtatgga ttacacgtag ttgaaaatat tcctttagta
     5881 ataccaccaa atccttacaa cgaattttac ctaaaaacta aacacgaacg catgggacac
     5941 atcaaccttt tcaaccccaa aaaggactaa aacccaatgc cagcaaagga atcaccccgt
     6001 attgtttaca tgggcacgcc cgagtttgcc gttgaaccac ttagggcgat tattgaagca
     6061 gggtacaacg tgtcagctgt agtcactgtt ccagataaac ccgcaggccg aggtttaaaa
     6121 cttcagcctt caccggttaa ggtatttgcc gagcaaaacc atattcccgt acttcagcca
     6181 gaaaagctca aggatccgca gttcattcaa acactgaaag agataaatcc cgatattgcc
     6241 gttgttgttg cctttagaat gttaccaagg gatgtttggc aaattcctcg tttaggaacc
     6301 tttaaccttc atgcttcgct cttaccccag tacaggggcg ctgcacccat taattgggca
     6361 atcataaatg gtgaaaagga aaccggaatt accacttttt tacttgatga acagattgat
     6421 acgggtaata tccttcttca aaagagtgta cccataggcg aaaccgaaac cgccggcgaa
     6481 ctccacgata ggctaatgta tcagggggca ggacttgttg ttgaaactat atgcatgctt
     6541 actgctggca acatcaatcc aattcctcaa aaccaaatca agataagtgg agagctaaaa
     6601 ccagccccaa aaatttttaa ggaaacctgt cgtgttgatt ggaacaatcc atcccaaaaa
     6661 gttttcaacc acattagggg gcttagccca taccctgctg catggactga acttaaaacc
     6721 aaaagaaaca atgttgaaac aattatacct ataaagattt accaggcgga aaaacttgac
     6781 caggctcaat cacaaaggcc tggaatgtta aaaaccgatg gaaaaacaca cctccatgtt
     6841 ttttgttcac agggtgcaat tgcgctaaaa actgttcagc aggcaggtaa aaaagccatg
     6901 ggaattgaag agttcctaag gggatggcag aatactgagc ttatcgaagt actataacgg
     6961 tttacgtttc ctcaagtaaa ttagttgacc cacctctggt tcatcgcccg gtttcattct
     7021 atttttccgg taaagtgact ttaacttaac gccgtacatc tgcgaaattt tatacatggt
     7081 ttcgccctct tccgctgtat gaaccggatt cgacctatct gctttccatc gtttgggctg
     7141 tatgtatagt tcctgcccag gacgaatcac tgaatcgcga gtcaaatcgt tgtacctgta
     7201 aagctgccag ggcattaaat ctaactccct agctaacgat tcaaaagtat cgccatcctt
     7261 ggcaacaata tattttaccc tgtttcggat gaaaacaggc cgagttttgt aaaggtcaat
     7321 ttcgtaatta tcccaatcgg ttacaaccgg tttgggtggc ttaatggcaa cggtaattcc
     7381 ctgatcgaat cggtaaagct cattgtcctc aattatctta ataagcagct ctgcatactt
     7441 tgggtttgta gcataaccag ctttcttgag cccatgggcc catgccttat aatcggttgg
     7501 ggtcaaatcg aataggaaag catagcgctt tccgcctctg agaaaaagac tatggtcgtt
     7561 aaacgattgc tccggatttt tgtattttct aaagcactcc ccttttcggt cgtcatcgtg
     7621 gtaaatggtt gcaccagtcc agtccttatg gcactttatc ccaaagtggt tattggcctt
     7681 tacggccaat gtgctattcc cattatcgct ttccagcatg gcctgggcaa gagtaatact
     7741 tgcaggtacg ccaaactgct tcatattatt aacggcaata tgcgaataca tgctaatgta
     7801 ttcctcgcgc gaaatcttgt tttgagaaaa tacagataga tgaatccctg taaggataaa
     7861 aaacagtgca actaaaatcc tattcatagc taaaactact ccaagcctaa caacaaaaac
     7921 tcactccata acttctcgct gggaggagga gctacaattt ttatttcttc cccagaaatt
     7981 ggatgggtaa atgcaaaaac ccttgaatgc agatcgatac cgccatcggg gttagaacgg
     8041 ggatagccat attttaaatc gcctcgaatt ggacatccaa ttttcgagag ctgagcacgt
     8101 atttggtgat gcctaccggt aatcaaatct attactaaca ggtagtaatt ttcagttcta
     8161 ccaacaagct catacttaag cttggcaatt tttgagccgt gcacctcacg gtgatataca
     8221 aacgacttat tttttttgtt atcgcgcaaa aggtaatgaa caagggtttg cgattcaaca
     8281 gggggttggt tctttacaat agcccaatac tttttggtga ttttcccttc ctgaaaaaga
     8341 ttattgatac ggcttaaccc tttgctggtt ttggcaaaaa gcactactcc actcacaggc
     8401 ctatcaatcc ggtgggtcac ttccaggaat acgcgacctg gcttactgtc gcgctcttta
     8461 ataaaccgct ttaccgtttc aaccaacgac tcatcgccgg ttttgtcggc ctgcacaatc
     8521 tcaccacagt tcttatttac cgctaaaaaa tggttatcct cgtaaaggat tctgtcgctt
     8581 gaaaaattta tcatcttcag tattagtatt gctcacgttc attgggaaaa tcgagcgatt
     8641 tcacatcgct aatataggct tgaaatgctc caagcatttc agcgtaaagg ttgtggtacc
     8701 gcctgagaaa gcgtggtgag aactcctggt taataccaag catatcgtgg gtaactagca
     8761 cttgaccatc aactccactg ccagcaccaa ttccaattac gggtattttc agctccgagg
     8821 caacctgttt tcctaatgcg gcgggaattt tttcaagcac aatggcaaaa cagcctgttc
     8881 tttccagcaa atgtgcatct tcaaccaatt tcttggcctc agcttcctcg cgtgccctta
     8941 caacgtaagt tccaaactta tggattgact gaggggtaag ccctagatgc cccataaccg
     9001 gtatccctgc agataggatt cgctccacgg agtcaataat ctccgagcca ccctcgagct
     9061 taacggcatc ggctccgctc tccttcataa ttcgaatggc cgaacgtaat gctaatttgg
     9121 agttgccctg gtaggttcca aagggcatat caacaacaac caatgcacgc tttactccac
     9181 gtaccactga actggcatgg taaatcatct ggtcaagggt gatagggagt gttgatatat
     9241 ggcctgccat tacattcgaa gcagagtcgc ctaccagcaa tacgtcaatt ccagcggcat
     9301 cgagtatccg ggccattgaa tagtcgtacc ctgtcagcat tgcaattttt tcacccctga
     9361 ccttcatttc ctgaagcaca tgggtggtta ccactttaac tttactctcg ctcgacataa
     9421 gtattaaact ttaaggattg tcaaaggtaa tctattattc aggaaaacct aagctaattt
     9481 attaaaattg aaaaaactga caaaaagaca caattaatga ctactatctg aaaacctgaa
     9541 ccttataaat atatcaactc aaatataaac acagaaacct gacaaaggat tactgaaaaa
     9601 ctaaaaattt tgtgacagat tttcgtcttt cagtccattt ttggtgttgg cacaatgatt
     9661 gaacaaagtg aaaggaatgt ttaaaaacga aacaaatcaa aagtataaca ataaaaatct
     9721 aaagcaatga gcaaaataat tggaattgac ctaggaacaa ccaactcatg cgttgccgta
     9781 atggaaggta acgaaccggt tgttataaca aacagcgagg ggaaacgcac taccccatcg
     9841 gtagttgctt ttgccgaaaa cggtgaacgc aaggttggcg acccagcaaa gcgccaggcc
     9901 atcattaacc cccaaaaaac catattctca attaagcggt tcatgggcga aacctacgat
     9961 aaggttgcac aggttgaagt accacgtgta ccctataagg tggttcgtgg cgaaaataac
    10021 acaccccgag tagatattga tggccgcctg tacacccctc aggaaatttc atcgatgatt
    10081 cttcagaaaa tgaagaa
//
LOCUS       DAOOWJ010000095        10084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6676_length_10084_cov_10.457573, whole genome shotgun
            sequence.
ACCESSION   DAOOWJ010000095 DAOOWJ010000000
VERSION     DAOOWJ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6676_length_10084_cov_10.457573"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            617..760
                     /locus_tag="PLW22_09135"
     CDS             617..760
                     /locus_tag="PLW22_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74904.1"
                     /translation="MRHFFKYLDVKFGKEYVFGFVIVVMLLFGIAALRGYLKEEKDSN
                     KSK"
     assembly_gap    1175..1274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1314..1387)
                     /locus_tag="PLW22_09140"
     tRNA            complement(1314..1387)
                     /locus_tag="PLW22_09140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1351..1353),aa:Arg,seq:tct)
     gene            complement(1454..2113)
                     /gene="hisIE"
                     /locus_tag="PLW22_09145"
     CDS             complement(1454..2113)
                     /gene="hisIE"
                     /locus_tag="PLW22_09145"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOU74905.1"
                     /translation="MTTLNFDKLNGLLPAIIQDAATGKVLMLGFMNREALEVTRKTGL
                     ATFYSRTRRQIWTKGETSGNFLKVTEIIPDCDGDTLLVKVTPQGPVCHTGNDTCFNET
                     NGNTLGFIDYLEEIIENRKQASADQSYTAKLLAAGTKRIAKKVGEEAVELALEAENGT
                     DERLLDEAADLIYHTLVLLSSRNLKFSKVIETLRTRHTDSKVSNSEVEDFKRGEKQWG
                     K"
     gene            complement(2128..2883)
                     /gene="hisF"
                     /locus_tag="PLW22_09150"
     CDS             complement(2128..2883)
                     /gene="hisF"
                     /locus_tag="PLW22_09150"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOU74906.1"
                     /translation="MLAKRIIPCLDIKNGRTVKGVNFENLADAGDPVYLAEKYYNEGA
                     DELVLLDISATLEGRETFIEVVRNVAKAIAIPFTVGGGISSVDQAERLIKAGADKISI
                     NTAAIQKPELIGKMAYAFGRQCVVVAIDTMLTDKGWEVVSHAGTKRTGIKALEWAKTC
                     EILGAGEILLTSHTSDGTREGFAVDITRKISQSISIPVIASGGAGLPEHFAEVLTTGM
                     ADAALAAGVFHYGVLSIPQVKKYLQSRNIEVRY"
     gene            complement(2865..3581)
                     /locus_tag="PLW22_09155"
     CDS             complement(2865..3581)
                     /locus_tag="PLW22_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOU74907.1"
                     /translation="MLVVPAIDIIDGKCVRLFQGDFLRVKEYDLNPVDQAKVFADMGL
                     EHLHLIDLDGAKDGAPRNLRVLEQIVKSTSLKVDFGGGLRDLNSVNLAISAGAWKVNL
                     GSMLLQANEWFMNDIPTQKVIAAVDCEHFMVKTNGWQNQTRVRVDELLNRLLSLRIEH
                     FTITDIGRDGTLTAPAFSLYEDLRQKFPDITIWASGGVISVADVINLKKMAINGAIVG
                     KAYYENKINLKELLQCLQNE"
     gene            complement(3575..4171)
                     /gene="hisH"
                     /locus_tag="PLW22_09160"
     CDS             complement(3575..4171)
                     /gene="hisH"
                     /locus_tag="PLW22_09160"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOU74908.1"
                     /translation="MKTNITIGIIQYGAGNQASVMNALDHLGVKNIPIVTPLDISKAD
                     KLIIPGVGHAKRAMEQLKETGLDAALQEATIPLLGICLGMQLLGEKSEEGDATCLRLC
                     SFNTVKFNLSLKIPQMGWNKVTLGSNPLFSGLPQEEWFYFVHSYYVSKNQYTIATCNY
                     GIEFTAAVQHKNFWGVQFHPEKSGKVGLQVLKNFVELC"
     gene            complement(4174..5256)
                     /gene="hisB"
                     /locus_tag="PLW22_09165"
     CDS             complement(4174..5256)
                     /gene="hisB"
                     /locus_tag="PLW22_09165"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOU74909.1"
                     /translation="MKPILFIDRDGTLIKEPTDYQIDSFEKLDFMPGVFSWLGRICKE
                     FDFYLVMVTNQDGLGTSSFPEDGFLGPHQVMLRALNAEGIQFDEILIDRSFPDENLPT
                     RKPGIDLVKHLLNDDFDIANSFVIGDRETDELFARNIGCQAIRLGTHATIPGSFTAMG
                     WEEVYNLVKPKRLAAISRKTNETDIAVNINLDGTGQAKVNTGIGLFDHMLEQLAKHSG
                     IDIDIVCLGDLRVDEHHTIEDVGITLGEALRKALGDKAGISRYGFALPMDESRAMVLL
                     DFGGRPYLVFEGSFSREFVGQMPTEMVKHFFHSLAYSAQVTLHIDFDGENDHHKVEAI
                     FKAFARSLGMAIKKDGNSIPSTKGII"
     gene            complement(5253..6314)
                     /gene="hisC"
                     /locus_tag="PLW22_09170"
     CDS             complement(5253..6314)
                     /gene="hisC"
                     /locus_tag="PLW22_09170"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOU74910.1"
                     /translation="MMTLSETLELVRPNIMRLKPYSSARNEFTGGNAILLDANENPYN
                     LWNDDLIVNRYPDPLQREAKLKIAEIKGVSPEQIFLGNGSDEAVDLIIRCFCEPSTDS
                     IVIFSPTYGMYSVCAEVNNVQVKEFILNNDFSINLQQFFNGISPSTKVVFICSPNNPT
                     GNAQPIETIEQILSKFRGIVVVDEAYIDFCPEKTYLSLLNRYPNLVVLQTFSKAWAMA
                     GARIGIAFANPKIIATLNKVKFPYNIGVPSLSFLSRAIEQYSNTKSLISQIVSERELL
                     RLRLQQLSCVKMVYPSDSNFLLVKTTDANSVYNYLLNNGIVVRNRTHEPLCNNCLRIT
                     VGKPYENSKLIQILNTYRP"
     gene            complement(6319..7596)
                     /gene="hisD"
                     /locus_tag="PLW22_09175"
     CDS             complement(6319..7596)
                     /gene="hisD"
                     /locus_tag="PLW22_09175"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOU74911.1"
                     /translation="MVTITDKTNINYSELLKRPVEYNPEIRTTVQEIIEKVRVQGDSA
                     LRLLTKKFDGIDIDRFEVDEQTIASSRNQVGENLKAAIEIAARNIERFHAAQKVSDVE
                     VVTMPGVSCKLVYRPLERVGLYVPGGTAPLISTVLMLGVPAKVAGCNELIVCTPPPVN
                     PIILYACSIVGAKVFTVGGAQAIAAMAFGTETIPRVDKIFGPGNSYVTEAKMQVSAMG
                     IPIDIPAGPSELLVIADESAIPQFVAADLLSQAEHGADSQVVLISNCNSIINETLDEI
                     NKQIKNLPRMDIALKALENSYAIKVETVERAIEISNTYAPEHLILAVASPEKYETMIK
                     NAGSVFMGNYTPESAGDYASGTNHTLPTSGFARSWGGVNLLSFTKTITYQSITPEGIQ
                     ELGNCIVEIANAERLTAHANAVKLRMEKLSNQS"
     gene            complement(7608..8468)
                     /gene="hisG"
                     /locus_tag="PLW22_09180"
     CDS             complement(7608..8468)
                     /gene="hisG"
                     /locus_tag="PLW22_09180"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOU74912.1"
                     /translation="MNTKITLAIQKSGRLNEGTIELLNRCGIRISNGNNHLKALAEGF
                     PMEVLLLRDDDIPQYVADGVADIGILGYNEVLEKGKDIEVIRELGFSRCRLSIAVPRD
                     VNYSGRQWLSGKRIATSYPEILKTFLNQNAIEADIHTISGSVEITPGIGMADAIFDIV
                     SSGGTLLSNNLKEVDVIMKSEAVLVANKSLDEQKSELLKKLEFRIEAVRKAQRNKYIL
                     LNAPKDKLNQIISLIPGMKSPTVLPLAIEGWCSLHSVVNEDDFWEVIGRLKDAGAEGI
                     LVVPIEKMVI"
     gene            complement(9300..9482)
                     /locus_tag="PLW22_09185"
     CDS             complement(9300..9482)
                     /locus_tag="PLW22_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74913.1"
                     /translation="MGTPRVELPRDTIRHACSVAHMVSLTRPTATFHPRPIAVSGFYA
                     SACFFRSIPPQGKGLG"
     gene            complement(9898..>10084)
                     /locus_tag="PLW22_09190"
     CDS             complement(9898..>10084)
                     /locus_tag="PLW22_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74914.1"
                     /translation="PMGTPRVELPRDTIRHACSVAHMVSLTRPTATFHPRPIAVSGFY
                     ASACFFRSIPPQGKGLG"
BASE COUNT         2613 a         2154 c         2143 g         3074 t
ORIGIN      
        1 tttgtcacgg actacaagtc cgtgccagca atgagtgacg tatgccgcac gcgtttaaag
       61 acgcgcgcga gcgagggtaa agtttaagat aattcttttg aacacgcgcg ccacctcttg
      121 ttagcgcacg tctgcggacg cgttcgatag cgaagttgga gagaccatag gtcaaacgca
      181 gtgccttggc cagacaaaaa aagtttttgt acgtgcctga acaaggaaat ttttttctac
      241 atttactaaa ggttgctggg cggataggtg tatgatgcac gcgtttaaaa acgtgcacga
      301 acattagaaa agtattttaa tatcattagt gaaaaacttg aggtatcacc agaatttgta
      361 acgaaacgtt tattaaaata cggtattaat aaaatcttta ttgaaagtct atatgaaagt
      421 ttaacaaaag ctactgttaa acaacaaatt gataaatcaa tttatgaata tgagcaaaga
      481 aaaacagaat aaagacttaa aaagtttgca ggaaaatctt ttaggttttt ttgtttctgg
      541 atttatattg ttaatgctgt tttttttcta tgattaaggt atttatcagg aaggaataag
      601 tacaaaatca aaagcaatgc gtcatttttt taaatattta gatgtcaaat ttggcaaaga
      661 atatgttttt ggttttgtta ttgttgtgat gctattattt ggaatagctg ctcttagggg
      721 ttatttgaaa gaagaaaaag atagtaataa aagcaaataa taaaccagct cgcgcggatt
      781 tgtaatccgt gcggttctag ttttcaatga ggcacatctc gaatgaataa taaagtgtgt
      841 aagaggtatt tgtcacgggc tacaagcccg cgccagcaat gagtggcata tgcagtacgc
      901 gtttacaaac gcgcgcgagc gatgggcctt cggctcatga ttccaccctg ttcttgacac
      961 taaaaggaga aggctatcca actataggat agcctttggt aaatgtgagt tttttcaagt
     1021 gatccgggcg ggattagttc gccttcggct catgatcccg ccctgatgtt tttctaagaa
     1081 tttaaggcta tccttgttca gaatagcctt tggtaaatat gagttttttc aagtgatccg
     1141 ggcgggatta gttcgccttc ggctcatgat cccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnntgttca gaatagcctt tggtaaatat gagttttttc aagtgatccg
     1321 ggcgggattc gaacccacga cccacagctt agaaggctgt tgctctatcc aactgagcta
     1381 ccggaccggc ttcgatattt cgaagcgcaa aattaatcat tactttcaaa aaataaaaca
     1441 ccgtacatca tttttacttg ccccactgtt tttcgcctcg cttaaaatct tcaacctcac
     1501 tgttgctcac ttttgaatcg gtgtgccggg ttctgagtgt ttcaataact ttactgaatt
     1561 taaggttacg gctcgatagc agaaccaggg tgtggtaaat caaatcggcg gcttcgtcga
     1621 gcaaccgctc gtcggtacca ttttcggcct caagcgcaag ctcaacggct tcctcgccta
     1681 cctttttggc tatgcgtttg gtgcctgcag ctaaaagttt ggctgtgtag ctttggtcgg
     1741 cacttgcttg cttacggttt tcaattattt cctcaaggta atcaataaag cctaaggtgt
     1801 taccatttgt ctcattgaaa caggtatcgt ttcctgtgtg acacaccggc ccttgtggtg
     1861 taacctttac cagaagcgta tcgccatcgc aatcgggaat tatctccgtc accttaagaa
     1921 aatttcctga ggtttcgcct tttgtccata tttgtcggcg tgttctgctg tagaatgtgg
     1981 caagccccgt tttacgggta acttcaagtg cttcgcgatt cataaaccca agcataagca
     2041 ctttacctgt ggctgcatcc tgaataattg caggaagcaa tccatttagt ttgtcaaaat
     2101 ttagtgtagt catagcatct gtttttatta gtatctaact tcaatatttc tgctttgtaa
     2161 gtattttttt acctgtggaa ttgaaagtac gccgtaatgg aacacgcctg cagccagggc
     2221 tgcatcggcc atacctgtgg ttaatacttc tgcaaaatgc tcaggcaatc ctgcacctcc
     2281 cgatgcaatg acaggaatac tgatgctttg ggaaattttt cgggtgatgt caactgcaaa
     2341 cccttccctt gttccatcgg atgtgtgtga agtgagtagt atttcaccag ctccaagtat
     2401 ttcgcaggtt tttgcccatt caagggcttt tatgcccgtt ctttttgttc ctgcatggct
     2461 tacaacctcc cagcctttat cggtaagcat ggtatcgatg gcaacaacca cacattgcct
     2521 tccaaaggca taggccattt tgccaataag ctctggtttt tgaattgcgg cagtgttaat
     2581 ggatatctta tcggcgccgg ctttaataag cctttcagct tgatctactg aacttattcc
     2641 cccaccaaca gtgaaaggaa tagctatagc tttagccaca ttgcgcacaa cttcaataaa
     2701 ggtttcgcgc ccttcaaggg tagcggagat atcaagcagc actagctcat cggctccttc
     2761 gttgtaatac ttttcggcca gatatactgg atcgcccgca tcggcaaggt tttcaaaatt
     2821 cacaccttta acggttcggc cattcttgat gtcgaggcat ggtattattc gttttgcaag
     2881 cattgtagta gttcttttag gttaatcttg ttctcgtagt atgctttacc aacaatagct
     2941 ccatttatgg ccattttttt aaggttaata acatcagcta cagagataac ccctccgcta
     3001 gcccaaattg ttatatcggg gaatttctgt cgtaaatctt cgtaaaggct aaaagcgggt
     3061 gcggttaatg taccatcgcg gcctatgtcg gtaatagtga aatgttcaat tctcaaactt
     3121 aataggcgat ttaaaagctc gtcaactctc accctggttt gattttgcca cccattagtt
     3181 ttaaccataa agtgctcaca atccactgcg gctataactt tctgggttgg tatatcgttc
     3241 ataaaccatt cattggcctg taaaagcatt gaaccgaggt taaccttcca tgccccagcg
     3301 ctgatagcaa ggtttactga gtttaagtca cggagtcccc ctccaaaatc gacctttagg
     3361 gaggtggatt ttacaatttg ttccagaacc ctcaggttcc tgggagcgcc atcttttgca
     3421 ccgtcaaggt cgataaggtg taggtgttcc aatcccatat cggcaaatac tttggcttgg
     3481 tctacgggat ttaaatcata ctccttaact cttaaaaagt cgccctggaa tagcctaacg
     3541 catttcccat caattatatc gattgctggc actactaaca tagctctaca aagtttttaa
     3601 gtacttgtaa tcctactttt ccgctttttt cggggtgaaa ttgaactccc caaaaatttt
     3661 tatgctgaac tgctgcagta aattcaatgc catagttaca agtggcaatc gtgtactggt
     3721 ttttagaaac atagtagctg tgaacaaagt agaaccattc ctcttggggc aatccactaa
     3781 acaaaggatt gctgcctagc gttactttgt tccaacccat ttggggtatt ttaagcgata
     3841 aattaaactt aacagtatta aagctgcata acctcaggca agtggcatca ccctcctcgc
     3901 tcttctcccc aaggagttgc atacccaggc aaatgccaag caacggaatg gtggcttcct
     3961 gtaaagcggc atcaagccca gtttccttta actgttccat tgctcttttg gcatgtccaa
     4021 ccccgggaat aataagtttg tcagctttgc tgatatccag tggcgtgaca attggaatat
     4081 ttttcactcc taagtggtcc agagcattca ttaccgaagc ctgattgccg gctccgtatt
     4141 gaattatacc tatggtaata ttcgttttca tagttaaata ataccttttg tgctgggaat
     4201 actattacca tcctttttaa ttgccattcc aaggctccgt gcaaatgcct taaaaattgc
     4261 ttccacctta tggtggtcgt tttcgccatc aaaatcgata tgcaatgtta cttgggcgga
     4321 gtaggcaagc gaatggaaaa agtgcttaac catttcggtt ggcatttgtc ctacgaattc
     4381 gcgagaaaaa ctgccctcga ataccaagta tggcctaccg ccaaagtcaa gcaacaccat
     4441 tgctctgctt tcgtccatgg gtaaggcaaa tccgtatcgc gaaataccag ccttatcgcc
     4501 tagagccttt cgtaacgcct cgccaagagt aatgcccaca tcctcaatgg tgtggtgctc
     4561 gtctacccgt aaatcgccaa ggcatacaat gtcaatatct attcctgagt gcttggcgag
     4621 ttgctcaagc atgtggtcaa agagtccaat acctgtattt acctttgcct ggcctgttcc
     4681 atcaaggtta atgtttacag cgatgtcagt ttcgttggtt ttacgtgaaa ttgcagcaag
     4741 ccttttaggc tttactaggt tatatacttc ctcccagccc atagctgtaa aactacctgg
     4801 tatggttgcg tgggttccaa ggcgaattgc ttggcatcca atgtttctgg caaatagttc
     4861 atcggtttcc ctatcgccaa taacaaagga gttggcaatg tcaaaatcat catttagaag
     4921 atgtttaacc aggtcaatac ccggtttgcg tgtgggtaaa ttctcgtcag ggaaggaacg
     4981 gtcaataagt atctcatcga actgaatacc ctcggcatta agtgctctaa gcattacctg
     5041 gtgtgggccc aaaaaaccat cctcagggaa actggacgtt ccaagaccat cctgattggt
     5101 taccattacc aggtaaaagt caaattcctt gcatattcgt cctagccatg aaaacactcc
     5161 aggcataaag tccagctttt caaagctgtc aatttggtaa tctgtgggct cctttattaa
     5221 ggtgccatcg cggtcgataa aaagtattgg tttcatggtc tgtacgtgtt aaggatctga
     5281 attaacttgc tattttcata gggtttaccc actgtaatac gtaaacagtt attgcataat
     5341 ggttcatggg tgcggtttcg gaccactatt ccattgttca gcaaataatt atatactgag
     5401 ttagcatcgg tagtctttac cagcaaaaag tttgaatccg aaggataaac cattttaaca
     5461 cacgacaatt gctgaagtct taatctcaaa agttcccttt cgctaactat ctgacttatc
     5521 aacgattttg tgtttgaata ttgctctatg gctctagata gaaatgaaag cgagggaaca
     5581 ccaatgttgt atgggaactt aaccttgtta agtgtggcaa ttattttggg gtttgcaaaa
     5641 gcaatcccaa ttcgagctcc tgccatagcc caagcctttg aaaaggtttg caaaacaact
     5701 aggtttgggt aacggtttaa gaggctaaga taagtttttt cagggcagaa atcgatgtag
     5761 gcttcatcaa ccacaactat accacgaaat tttgatagaa tttgctcaat tgtttcaatc
     5821 ggttgagcat tgccagtggg attgttcgga ctacatatga atacaacttt ggtgctaggt
     5881 gatataccat taaagaattg ttggaggttg atactaaaat cgttgttcag aataaattct
     5941 ttgacctgca cgttgttaac ctcagcgcaa accgagtaca tcccataggt tggtgaaaat
     6001 attacaatgc tatcggttga tggttcacaa aagcaacgga ttatgaggtc aactgcttca
     6061 tcgctaccat tgcccagaaa aatttgctca ggtgaaactc ccttgatttc tgctattttc
     6121 agttttgctt cacgctgcag tgggtcgggg tagcggttta caatcaaatc gtcgttccaa
     6181 aggttgtaag ggttttcgtt agcatcgagc aaaatagcat tgcctccagt aaactcattt
     6241 cgcgccgatg agtaaggttt tagcctcata atatttgggc gaaccagttc taatgtctca
     6301 cttagtgtca tcatggtgtt aactttgatt acttagtttt tccattctta gcttaactgc
     6361 attggcgtgt gcagtcaatc gttcagcgtt agctatttca actatgcaat taccaagttc
     6421 ctgaatccct tcaggggtaa ttgactggta agtaatggtt tttgtgaacg aaagcagatt
     6481 tacgccaccc caacttcttg caaatccgct tgtaggaagg gtatgattgg ttccgcttgc
     6541 ataatcgcct gccgattcgg gtgtgtaatt ccccatgaaa accgaacccg cattctttat
     6601 cattgtctca tatttttcag gacttgcaac tgcaagtatc aggtgttccg gggcataggt
     6661 gttgcttatt tcaattgctc gttcaactgt ttccacctta atggcatagc tgttttccag
     6721 cgcttttagc gctatgtcca ttctaggtag atttttaatc tgtttgttaa tctcatctaa
     6781 agtttcgtta ataattgagt tgcaattgct tattagcaca acctggctat cggccccatg
     6841 ctctgcttgc gataggaggt cggcagcaac aaattgtgga atggcacttt cgtcggctat
     6901 tacaagtaac tccgatggtc cggcaggtat atcaatagga attcccatgg cagaaacctg
     6961 catttttgct tcggttacat agctattccc cggcccaaaa atcttatcaa cccttggaat
     7021 ggtttcagtt ccaaatgcca tggcagctat ggcttgtgca ccaccaactg tgaatacctt
     7081 tgccccaact atgctacaag cataaagaat tattgggttt actggtggag gagtgcaaac
     7141 aataagttca ttgcatccgg ctacctttgc agggacaccc agcattagca ctgtcgatat
     7201 tagtggggct gtcccacccg gaacataaag gcctacgcgt tccaggggtc ggtaaacaag
     7261 cttgcagctt acaccgggca tagtaaccac ttcaacgtcg cttacttttt gggctgcgtg
     7321 aaacctttca atgttccttg cggctatttc aattgcagct ttaagatttt ccccaacctg
     7381 attgcggcta cttgcaatgg tttgttcatc aacctcaaac ctatcgatat cgataccatc
     7441 aaactttttg gtcagcaacc gtagggcaga atcgccctga accctgacct tttcaataat
     7501 ttcctgaaca gtagttctta tctcagggtt atactcaacc ggtcgcttaa gtagctcaga
     7561 gtagttaatg ttggttttat cagtaattgt aaccatagct ttagctttta aataaccatt
     7621 ttttcgatag gcactacaag tattccctcg gcacctgcat ctttcagcct accaataact
     7681 tcccaaaaat catcctcgtt gactaccgag tgaagtgagc accacccctc gatagccaat
     7741 ggtaaaacgg ttgggctttt catcccaggt atcaggctaa tgatttggtt cagcttatcc
     7801 tttggagcat ttagtagtat gtacttgttg cgttgtgctt tacgcacagc ctcaattcgg
     7861 aactcaagct ttttaaggag ttcgctcttt tgctcatcta agcttttatt ggcaacaaga
     7921 acagcctcgc ttttcattat aacatcaacc tctttgaggt tgttgcttag caatgttcct
     7981 ccgctactta caatatcgaa tatggcatcg gccattccaa tcccgggcgt tatttctact
     8041 gaaccgctaa tggtgtgaat atcagcttca attgcgtttt gattcaggaa tgttttaagg
     8101 atttcaggat atgatgttgc tatccgcttt cccgatagcc attgcctgcc actatagttt
     8161 acatcgcggg gaacagcaat ggaaagccgg catcgggaaa agcccagttc acggataacc
     8221 tcgatatctt tccctttctc aagaacctcg ttgtatccaa gaatgcctat atcggcaacc
     8281 ccatcggcaa cgtattgcgg aatatcgtca tcccttagca gtagtacttc cattgggaaa
     8341 ccctcagcca acgctttaag atggttgttt ccgtttgaaa ttcttattcc gcatcggttc
     8401 agtagttcaa tggttccttc gtttaatcgg ccgctttttt ggatggccag agtaattttt
     8461 gtgttcatat caacttgttt ttggtgattt actaaatgca gaaagggtct gccttttgaa
     8521 tggcagaccc ttgaataagg tagtctcttt atcgatttac tattggttat accaaatgct
     8581 catctgccat tcgccagaag tgtgaatggt gatgatgatg aagagtggta taactagtag
     8641 ctatcatttg cggtttgttg ttctgcaaat aaaagtaact tttattaaaa atccaaatca
     8701 gcatagaaaa attaatgaaa caccattgga atgtagcttg tagaaaaaat gtgattcaat
     8761 gttatttagt taaggttatt tgtcatgttg cgcacaagcg aaacatcacg ctgtcgtgct
     8821 gagtcccgat ttatagggat tggcttcgcc gaagcatcac attgctcatt tttcttcaat
     8881 cctttgaaga gatccttcaa catagctcgg gatactaaaa accctatctt cctttttgct
     8941 cacttgtcat gctgagctag gcgaagcatc tctttgcctt aaaccctcta atacttgtta
     9001 attttttgga aactgctctt tgcggtgttt ttatattgcg atgcaatgcc tcacccttgc
     9061 tctttggcat atgcattgcg tgcatcgcta tacatatggg aataatcatg gtgttacaaa
     9121 tattacgccc ctacggggct ggaagcgttg ttagttgttc gttgctcgtg aaactattgt
     9181 catgttgagc gtaagcgaaa aatctcatgg ggccgattag agatccttcg ctacgctcag
     9241 gatgacaaat aaaattattc gtgtaagttg ctcgttgttc tttgatcgtg aaattgacct
     9301 caccccagcc cttttccctg agggggaatg gagcggaaga agcatgcgga ggcataaaag
     9361 cctgacacgg caatgggtcg ggggtggaac gttgcggtgg gtctagtaag cgataccata
     9421 tgagccacgc tgcaggcgtg gcgaatagta tcgcgtggca gttccacccg cggggtaccc
     9481 atcgggtcgg gcttttcagc cttgattttc tttggttctt tcttgtatca agacaagaaa
     9541 gaacgtgaaa ataattcttt gtaattattt ttcttttgcg atgcaacgct tcagtgttgc
     9601 tctctggaaa aggtgttgcg tgcatcgcaa aataatttga acacctcaat accctgggtt
     9661 aaaaccctgg gttaatgtga tttgcccttt cagggcgaat gatgcacgcg tcgggatacg
     9721 tgcgcaaacg agagggctgg aagcgttgtt agttgttcgt tgcacgtgaa gttattgtca
     9781 tattgagcgt aagcgaaaaa tctcatgggg ccgattagag atccttcgct acgctcagga
     9841 tgacaaataa aattattcgt gtaagttgct cgttgttctt tgatcgtgaa attgacctca
     9901 ccccagccct tttccctgag ggggaatgga gcggaagaag catgcggagg cataaaagcc
     9961 tgacacggca atgggtcggg ggtggaacgt tgcggtgggt ctagtaagcg ataccatatg
    10021 agccacgctg caggcgtggc gaatagtatc gcgtggcagt tccacccgcg gggtacccat
    10081 cggg
//
LOCUS       DAOOWJ010000096         9964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6759_length_9964_cov_7.325764, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000096 DAOOWJ010000000
VERSION     DAOOWJ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6759_length_9964_cov_7.325764"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..2822
                     /locus_tag="PLW22_09195"
     CDS             276..2822
                     /locus_tag="PLW22_09195"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU74915.1"
                     /translation="MRAKVVSITLLALTFAFFTVNASERDSLLNLLSASKGKDKVEIL
                     IKLTEATSGTDEAIKYADEAIVAARKINDELLAKAYLNKGYALSENYQDNDAIEYYRK
                     ALELAQKNGFKSSILEAYKGISTSYFYIDSFGESQRYASLLLQQALELDSLRYQAYAY
                     FRLGKILQEQGKNDSALHYIRRSLGLREKLGDWKEIALTYNGLGRIYFDLAQYDSAIW
                     CYSKEVELKEQMGDQPAYLAIAYLNIGRTHIAISNFQLALEQYQKALRTFESINHQEG
                     IATCNSGLGMIYENLSQSILATDENEANYLKALGYHKTALELFHNLQKKKEEGQTLQN
                     IGNVFSRLATNRYVARFGEMWEDSLYKIKQTEILASFDSARHYYNKALSIFEELHEEG
                     EIAKVNTNIGSIYSWARDWEKANTYINRALQLARKNNLLYETTAALYAKGESLFRQGR
                     FDETEIVFIECSRLSEKLGLKETLRYSYERLSKLYEHRGDLVRAYAYFKQAVKIKDEI
                     FSEKSQRVMAEMQTKYETEKKEQQLVLMKNKDELQKSIIQRQKLMIIGAIAGSFLILM
                     VALLMFKMFRDKQRANIILEEKNALITRQKQEITDSIKYASRIQTAVLPTSALISDVL
                     PDHFVLFLPRDIVSGDFYWMTRKNEKIVLVAADCTGHGVPGAFMSMLGVSFLYEIVNK
                     ESILQPAEILNNLRNHIKTTLSQTGKIEEQKDGMDISLCVIDKQNQKLEWAGAYNPLY
                     QISNGELIEYKADKMPVAVHINDFNLFTNHEIEYQPGDAFYMFSDGYADQFGGTDGRK
                     FMLKRLKETLLSIWEKPMAEQKEILHKIHMDWRGEQNDQVDDILIIGFRV"
     gene            complement(2812..4080)
                     /locus_tag="PLW22_09200"
     CDS             complement(2812..4080)
                     /locus_tag="PLW22_09200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU74916.1"
                     /translation="MWVKYFTKKGFSPKIDVNPSVNLGICVVIPCYNEPSLIESLGSI
                     AGCNLPACDVEVIVVVNHPEGVHYDVLENSNKAVKDIDEANRLWGCERLRFHAVKAFN
                     LPRKQAGVGYARQIGMDEAAFRLLTVGTKQGIIACFDADSQCMPNYLAELYELWMQYP
                     KTHACSVRFEHPLSGDEFPAEIYKGITAYELHLRYYNQASRFIGFPFAYHTVGSSMAC
                     SAEAYVKFGGMNRHQAGEDFYFLQKIIPHGHFRELNTTCVYPSPRPSNRVPFGTGRAM
                     SKLVYSREPISTYHLESFLNLEPLFQNVTTLYNYSGNQIERFIELLAYPLSDFLFQVD
                     AARAIENVKSNASSAKTFKKRFFLWFDAFMLLKYMNFAAERYYGRQPVEEQSKSLLAC
                     MGYKPLQTLRTRDLLLFYREMESVEWSAKL"
     gene            complement(4166..4771)
                     /locus_tag="PLW22_09205"
     CDS             complement(4166..4771)
                     /locus_tag="PLW22_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU74917.1"
                     /translation="MKKTNLIINILFGVAIVVLFVLHFTMGSKRGDVKGTEGNAVLTE
                     QFTAAWVNMDTLMNSYDMYFDMKKELEESGRKKEADLNAKSRSFEKEAYDFQDKVQKG
                     LLTRSEAQQLQTNLAAKEQQLYQLRDEMRMQLAEEEQVKLRLIHNSIIEYLKEYNADK
                     GYHLILSNTFGGPLLYGNPANDITKEVLEGLNKKYAADKKK"
     gene            complement(4915..5298)
                     /locus_tag="PLW22_09210"
     CDS             complement(4915..5298)
                     /locus_tag="PLW22_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOU74918.1"
                     /translation="MSLSFDPKDFDSIIVVGDRVLIKPKNPDERTKAGLLLPPGVQEK
                     QKAHSGYVVKVGPGYPIPMFTEADEPWKKSRNDAKYFPLQAQVGDLAVYLHENAIEVE
                     FNSEKYVVVPFSSILLLFRDEGLLE"
     gene            5464..5880
                     /locus_tag="PLW22_09215"
     CDS             5464..5880
                     /locus_tag="PLW22_09215"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOU74919.1"
                     /translation="MGSIEIFNSHYAVGIYNPEKKCVEVTWHGNQTIEEYKALFEALL
                     EFQRNSGLEIKGYLSDIRDQGVVNPDSRKWFEQVALPQAINQGLKWAAVVFDGNTFKR
                     YYLNLILQVANIYGLPLKFFNSKEDAYKWAETPNLK"
     gene            6213..8336
                     /locus_tag="PLW22_09220"
     CDS             6213..8336
                     /locus_tag="PLW22_09220"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOU74920.1"
                     /translation="MTQVPANIHEVPFVRLAIPLIAGILIQYYLNPLNANLTIGLILI
                     LLLLLIYDRIFIKSWEKRWIFGLIINLLLALFGSLLVTINRPETTFKSNSNYSMLVRL
                     TEPPVTGSNSIKVKAEIIDIKTYANLESPKPVLLYFLLSDSASHGLNYGDILALSGNL
                     KEFSEPPNPFQFNMKRYMYLLGIVYYIRVNQGCWEKVGSNPSKIFKLAYMAQSYVIET
                     LKGYGIKGQELAVISALMLGYKSLLDDEIRQVYSSVGAMHILAVSGLHVGLIFSVVVI
                     IITLLPKRRYWLAIRLGIALTVLWGYALVTGLSPSVCRATIMFSLFAIGQTAGLKTNS
                     YNTLAAAAFLLLVSNPFMLFNVGFQLSFAAVLSIVFFHPTIYSVLKPKSKLGDYIWSL
                     LSVSAAAQILTLPLTLYYFGQFPVVFLVTNLIAIPLATIALYLSLITIAFSLIEQLGF
                     LFAKILGFVTWLLNSGLKLVDTIPFSSISSIYFTSWQAILLLLSILMLSVYLVNRYRP
                     FLQYSLISLIGVLAISALHLVRVKNQAELVVFPVPKSSVICVNSNGNALLVLYDSIDT
                     KFSTDGYIRNRALQKALEINLNFYQEQEHSNQLKIIKKSGLALLVAGNKTIALAYNDS
                     ARFLKSIKPLNVDILIANRFYSNSLLNIITPQKVIIDPSIKSAYANRLSSTLIKNNIA
                     FYNTSVSGFFEYNLNETNPKTFASQ"
     gene            8414..9754
                     /gene="trkA"
                     /locus_tag="PLW22_09225"
     CDS             8414..9754
                     /gene="trkA"
                     /locus_tag="PLW22_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOU74921.1"
                     /translation="MYIIVVGAGEVGTHLAKMLAKEYHDVTVIDPDEEMLSHVTASAD
                     VLTIQGSATSIKVLKDANIKKADLFIAVAHSEETNIVSATLAKKLGAKKVIARIDKWE
                     YLQPNNKEIFLNLGIDSLIYPERLAAREIITLLGQTSSTEFVDFSGGKLSLIVFKLEE
                     ESPVVNYTLLQATKNTQNLDYRAVAISREGITIIPRGNDQFKVNDLVYVIANQNGIKE
                     MLEYSGKKNIDVNNLMILGGSRIAIHLATELERSMNVKLIEVDPEKSEKLAAQFKNAL
                     VINGDGRDTDLLMEEGLQYMDAFVAVTGNSETNILSCIIAKRMGVKKTIAEIENINYI
                     RLAESIGVDSVINKKLITASRIFRYTMSTDVSTIKCLTGSDAEVLEFIVKPGAPITKG
                     KLKDIGFPKDAIVGGVVRGDNVFIAKGDTEIKPYDRVVVFALPTAINKVGKYFN"
     gene            complement(9747..>9964)
                     /locus_tag="PLW22_09230"
     CDS             complement(9747..>9964)
                     /locus_tag="PLW22_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU74922.1"
                     /translation="TDYGVGMPRETADRLFNTVESLIQPGTMNERGSGLGLIICKQLV
                     TDLGGQIKVTSEQQRGSTFTVSLPNLS"
BASE COUNT         2942 a         2170 c         2051 g         2801 t
ORIGIN      
        1 atgtgtgtcg cttaaatcta tttctaacct gtagacttat ttcaggacta gacatttgat
       61 ttcgtttaac ggttcacggt tcacgtttaa cgaacaaaac tccctccaat tctacattcc
      121 gagtattccg tttcattccg cctcattccg cctcattcct caagaaatta gcccagctaa
      181 atttgataat tcatagcaca taggttcatt tttatcaaat tcctttgcat aatgctgtaa
      241 tctttagtaa tttgctcacg aatcattgtg ttaccatgag ggcaaaagtc gtatcaatca
      301 ccctattagc gctaaccttt gcatttttta cggtaaatgc aagtgagcgc gatagcttac
      361 ttaaccttct ttcggcctca aagggtaagg ataaggttga aatccttata aagcttaccg
      421 aggcaacctc cggcaccgac gaggccatta agtatgccga tgaggccatt gtggctgcgc
      481 gcaaaataaa cgatgagctg cttgcaaaag cttacctgaa taagggctac gccctatcgg
      541 aaaactacca ggataatgat gccattgagt actaccgcaa agcgcttgag ctggcgcaaa
      601 agaatggctt taaaagttcg attctggagg cctataaggg aatttctaca tcgtacttct
      661 atatcgattc gtttggggag tcgcaaaggt atgccagcct gttgcttcaa caagctcttg
      721 agcttgatag ccttaggtac caggcatacg catacttcag gctagggaaa atacttcagg
      781 aacagggcaa aaacgattca gccctacatt acattcgtcg ctccctgggg ttaagggaaa
      841 aacttggcga ctggaaggag atagccctta cctacaatgg gcttgggcgt atttactttg
      901 accttgccca gtacgatagc gcaatatggt gctacagcaa ggaggtagag cttaaggagc
      961 aaatgggcga ccaacctgcc taccttgcca ttgcctacct caacataggg agaactcaca
     1021 ttgccataag taactttcag cttgcccttg agcagtacca aaaggcactc cgtacttttg
     1081 aaagcataaa ccaccaggaa ggaatagcca cctgtaactc ggggttgggt atgatttacg
     1141 agaatctatc gcaaagcatt cttgccaccg acgagaacga ggccaactac cttaaagcac
     1201 tgggctacca taaaacagct cttgaactat tccataatct gcaaaagaaa aaggaggagg
     1261 gccaaaccct tcagaatatt ggcaatgttt tttcgcgatt agccactaac cgttatgttg
     1321 cccgctttgg cgaaatgtgg gaagactccc tttacaagat taagcaaacc gaaattcttg
     1381 cctcgttcga tagcgcaagg cattactaca ataaagccct tagcattttt gaagagctgc
     1441 atgaggaagg cgagatagcc aaggtaaaca ccaatattgg cagcatttac agctgggctc
     1501 gcgactggga aaaggcaaac acctatatca accgtgctct gcaactggca cgaaaaaata
     1561 atctgcttta cgaaaccaca gcagcccttt acgcaaaggg cgagagccta ttccgtcagg
     1621 gacgattcga tgagactgaa attgtattca ttgaatgttc cagactatcg gaaaaacttg
     1681 ggttaaagga aactctacgt tacagctacg agcgcctatc gaagctatac gagcatcgtg
     1741 gcgatttggt acgcgcatat gcatacttta aacaagcggt caaaataaag gatgaaattt
     1801 tcagtgaaaa gagccagagg gtgatggccg aaatgcaaac caagtacgaa accgaaaaga
     1861 aggaacaaca gcttgttttg atgaagaata aggatgaact ccaaaagtcg ataatccagc
     1921 gtcaaaagct aatgattatt ggcgctattg ccggttcgtt ccttatactc atggttgccc
     1981 tactcatgtt caagatgttc cgcgataagc aacgcgccaa tattattctg gaagaaaaaa
     2041 atgcgctgat tacccgccag aaacaggaaa ttaccgatag cattaagtat gccagccgca
     2101 ttcaaacagc tgttttgcct acatcggctt taatctccga tgtgctcccc gaccactttg
     2161 tgcttttcct gccccgcgat atagttagcg gtgactttta ctggatgacc cgtaagaacg
     2221 agaaaattgt actggttgct gccgactgta ccggacatgg tgtgcccggt gcgttcatga
     2281 gcatgctggg tgtgtcgttc ctttacgaga ttgtaaataa ggaaagcata cttcaaccag
     2341 ccgagatact taacaacctc cgtaaccaca ttaaaacaac cctaagccaa accggtaaga
     2401 tagaggagca aaaggacgga atggacattt cgctttgcgt aatcgataag caaaaccaaa
     2461 aactggagtg ggctggcgcc tacaatccgc tttaccaaat aagtaatggc gaacttattg
     2521 agtataaggc cgataaaatg cctgttgctg ttcacatcaa cgacttcaac ttgttcacca
     2581 atcacgaaat tgaataccag cccggcgacg ccttttacat gttctccgat ggatacgccg
     2641 accagtttgg cggtaccgat ggaagaaaat tcatgctaaa acgcctcaag gaaaccttac
     2701 ttagcatctg ggaaaagcca atggctgaac aaaaggaaat acttcataag attcacatgg
     2761 attggcgcgg tgagcaaaac gaccaggttg acgatatact gattattggg ttcagagttt
     2821 agctgaccac tccacactct ccatctccct atagaacagg agcaaatccc ttgtcctgag
     2881 cgtttgtaat ggtttatagc ccatgcaggc caaaaggctt tttgactgct cctcaactgg
     2941 ttgtctgccg tagtaccttt ccgcagcaaa gttcatatac ttcaatagca taaaggcatc
     3001 gaaccaaagg aagaatcgtt ttttaaacgt tttagccgat gatgcattgg attttacatt
     3061 ttcaatggct ctggcagcat caacctgaaa aagaaaatca ctcaaaggat aagccagcaa
     3121 ttcaataaac ctttcgattt gattaccact gtaattgtat agcgtagtaa cattctgaaa
     3181 cagcggttca aggttaagaa acgattcaag gtgataggta gatattggct cgcggctgta
     3241 aaccagctta ctcattgccc tgcccgttcc aaagggaacc ctgttcgagg gtcttggcga
     3301 agggtaaacg caggtggtgt taagttcccg gaagtgtcca tggggaataa tcttttgaag
     3361 aaagtaaaaa tcctcccctg cctgatggcg attcattccg ccaaatttta cgtatgcttc
     3421 agcactgcaa gccattgatg aacctactgt atggtaggca aacggaaaac caataaaacg
     3481 cgaagcctga ttatagtacc ggaggtgtag ctcataggcg gtaatgcctt tgtatatctc
     3541 cgcaggaaac tcatcgcctg aaagaggatg ttcaaagcga actgaacatg catgggtttt
     3601 tgggtattgc atccacagct cataaagttc cgcaaggtag tttggcatgc actgtgagtc
     3661 ggcatcaaaa caggctatga ttccctgttt cgtaccaacg gtaagcagcc taaaagccgc
     3721 ctcgtccata ccaatctgcc gggcatatcc aacaccagcc tgtttgcgcg gtaaattgaa
     3781 tgccttaacg gcatgaaatc gcaaccgctc gcaaccccat aatcggttcg cctcatcaat
     3841 gtctttgact gctttattgg aattctccaa cacatcataa tgtacccctt cgggatgatt
     3901 tactacaact attacctcaa catcgcaggc gggtaaattg caaccggcaa tcgaccccaa
     3961 cgattctatt aagctaggtt cattgtaaca gggaataaca acacaaatcc caaggttaac
     4021 cgaagggtta acatcaattt taggcgaaaa accctttttg gtgaagtatt ttacccacat
     4081 ggcgttgcaa aaatagcgaa accctttctc atgaaaaaca ttcgaaaggg tttcgggttg
     4141 gtttaggttt agtataggtg ggtatttatt tctttttatc agcagcgtac tttttgttta
     4201 agccctcaag tacttccttg gtaatatcgt tagcgggatt cccgtataat agcgggccac
     4261 caaaggtgtt actgagaatt aaatggtaac ccttatcggc gttgtactcc ttaaggtact
     4321 caatgatact gttgtgaatc aaccgaagct taacctgttc ttcttcggca agctgcatgc
     4381 gcatctcatc gcgaagctgg tatagctgct gctctttagc ggcaaggtta gtttgaagct
     4441 gctgagcctc ggagcgggtc aataaaccct tttgaacctt atcctgaaaa tcgtaggctt
     4501 ccttctcaaa gcttcgcgat tttgcgttaa ggtctgcctc tttctttcta ccgctttctt
     4561 caagttcttt tttcatgtcg aagtacatat cgtagctatt catgagggtg tccatgttaa
     4621 cccatgcggc agtaaattgt tccgttaata cggcattccc ttcggttcct ttcacatcac
     4681 ctcttttgct acccatagta aaatggagaa caaaaagaac aacaatggca acgccaaaca
     4741 gaatgttgat tattaggttt gttttcttca taattaattg atattactga ttagtatatt
     4801 tcaagttatt caaaacgcac attaaatcaa acatccacaa acatactcac gcaaatataa
     4861 atgaaaatga aacataacaa aatatttcag ccagtttttt cccatttaaa acgattattc
     4921 caataatcct tcgtcgcgga aaagtaataa aattgatgag aaaggaacaa ccacatactt
     4981 ctcgctgttg aactcaacct caatagcatt ttcgtggagg taaacggcca ggtcgcccac
     5041 ctgcgcttgt aggggaaagt acttagcatc gttcctactc tttttccaag gctcatcggc
     5101 ctcagtaaac atggggattg ggtatccggg tccaaccttt accacatagc cgctgtgggc
     5161 cttttgcttt tcctgaactc ctggaggtag caaaagccca gccttagtgc gttcatcggg
     5221 gtttttgggc ttaatcagaa ccctatcgcc tactacaata atactgtcaa aatctttggg
     5281 atcgaatgaa agtgacatat tactcattgt ttatcacagc aaaattatcg ttttgttttt
     5341 aaatgtacgt tttttgtact tagatttagt tattctatcc acaaaattat gatgtgtgtt
     5401 attagcttat tgacttgcac actgtatatt actatcttgc aactgacaac cattacaaca
     5461 tttatgggtt ctatagaaat tttcaacagc cattatgcgg tagggatata caaccccgaa
     5521 aaaaaatgcg ttgaagttac atggcacggg aatcaaacca ttgaggagta caaagcgctt
     5581 tttgaagcac ttttggaatt ccagcgtaac tccggattag agataaaagg gtatctttcc
     5641 gacatacgag accagggtgt agtaaacccc gacagccgaa aatggtttga gcaggtagcg
     5701 ctaccacagg ctattaacca gggcttaaaa tgggctgcag ttgttttcga tgggaataca
     5761 ttcaagcgtt actacctgaa ccttatcctt caggtagcaa acatttatgg attgccccta
     5821 aaatttttca attcaaagga ggatgcctat aaatgggctg aaacacccaa tctcaaatag
     5881 tttacaacta atcatattga cccttccctt taacccaagt gtcatgattt aacatattca
     5941 acctgactaa tttttagaag tttgagtcgg catgagtcgg aatagacagt atatgatgga
     6001 gttagagaaa gttagaggat ttcgtcaatt gattatttca attgacgaat cgactaagaa
     6061 atataatcat tcctagactc cggaatgaaa gattatgaaa agtcaggctt gcctcgataa
     6121 atcaatagaa acaacttgtt taaatttttt tgcgtttcta ttgaataatc tcaattaaaa
     6181 aagttgtaac ttgaattgtt gttacaacca atatgaccca agttcccgct aatatccatg
     6241 aggttccttt cgttcgactg gctataccgt taatagctgg tatccttatt cagtattacc
     6301 taaatccgct caatgccaat ttgacaatag gcctaatatt gattttacta ttgctgttga
     6361 tttatgaccg aatttttatc aaatcttggg aaaagcgctg gatttttggg ttgattataa
     6421 atcttctact cgcacttttt ggaagtttgc tggtaaccat aaataggcct gaaacaacct
     6481 ttaaatcaaa ttcaaactat agcatgctag taaggcttac tgagccacct gttacaggaa
     6541 gcaattcaat taaggttaaa gccgaaatta tcgatattaa aacatatgct aatcttgaat
     6601 cgcccaaacc ggtgttgcta tactttttac tgtcggatag tgccagccat ggattaaatt
     6661 atggcgatat attagcacta tcgggaaatc taaaggaatt ttcggaacca cccaacccct
     6721 tccagttcaa catgaaaagg tacatgtacc ttttaggaat tgtttactat atcagggtta
     6781 accagggttg ctgggaaaaa gttggaagta atccaagtaa aatcttcaag ttagcataca
     6841 tggcccaaag ctatgtaatt gaaaccctga aaggatacgg aataaaaggc caagaactgg
     6901 cagtaatatc agcgttaatg ctggggtata aatcgttgct cgacgatgaa atccgacagg
     6961 tttactcttc ggtaggggcc atgcatattc ttgccgtgtc gggtttgcat gtggggctga
     7021 tttttagcgt ggtggtaata atcatcaccc ttttacccaa gcgccgttac tggttggcaa
     7081 tacgattggg tatagctctt acagtactat ggggttacgc cctggttaca gggctttcac
     7141 catcggtatg tagggctacc attatgtttt ctctctttgc catagggcaa acagcaggct
     7201 taaaaaccaa tagctacaac accctggcgg cagcggcttt tttgcttttg gttagcaatc
     7261 cgttcatgct attcaacgtg ggctttcaac tctcgtttgc cgctgttctg tctattgtat
     7321 tttttcatcc taccatttac agtgttctta aacctaaaag taagcttggc gattatattt
     7381 ggtcgctctt gtcggtttcg gcggcagcgc aaatccttac cctaccccta acgctttact
     7441 actttggcca attcccggta gttttcctgg ttacaaatct tattgcaatt ccgctggcaa
     7501 caatagctct ttacctatct ttaattacga tagctttttc gttaatagag caattgggct
     7561 ttctgtttgc taaaattctt gggtttgtca cgtggctttt aaattcgggg cttaagctag
     7621 ttgacaccat tcccttttcc agcataagtt caatttactt taccagctgg caagcaatac
     7681 tgcttttgct ttcaatccta atgttatcgg tatatctagt aaatcgatac agaccttttt
     7741 tgcaatattc attaatttcg cttataggag tacttgccat tagtgcatta catttggtga
     7801 gggttaaaaa tcaggcggag ctggttgtat ttccggtacc caaaagttca gtaatatgtg
     7861 taaacagcaa cggaaacgct ctattggttc tttacgattc aatcgatacc aagttttcaa
     7921 cggatgggta catcaggaat cgggcgctac aaaaagccct tgaaataaac ctaaactttt
     7981 accaagagca agagcattca aaccaactaa aaattatcaa aaaatcgggt ttggcattac
     8041 ttgttgctgg aaacaaaacc attgcattag cctataacga tagcgcaaga ttccttaaaa
     8101 gcataaagcc cttaaatgtt gacatactta ttgctaatcg cttttatagc aatagtttat
     8161 taaacatcat aaccccacaa aaggtgatta ttgacccaag cattaaatca gcatacgcta
     8221 atcggctttc atcaacactg attaagaaca atatcgcttt ttacaataca agcgtttcgg
     8281 gcttttttga atataacctc aacgaaacga atccgaaaac ctttgcatct cagtaagtaa
     8341 taattattta tactcaggta cattttgatt aaaaaagatg ttactttgca atttattttt
     8401 gaatacataa cctatgtaca ttattgttgt tggtgcaggc gaggtaggaa cgcacttggc
     8461 caaaatgtta gccaaggaat accacgatgt tacagtgatt gaccccgatg aggagatgct
     8521 tagtcacgta acagccagtg ccgatgtact caccatacag ggttcagcaa catcgataaa
     8581 ggtattgaag gatgctaaca taaaaaaggc cgacctgttc attgccgttg ctcattccga
     8641 ggaaaccaat atagtctcgg caacccttgc caaaaagctt ggtgccaaaa aggtaattgc
     8701 ccgtatagac aagtgggaat acctacaacc caacaacaag gagattttcc ttaacctagg
     8761 catcgattcg ctcatctatc ccgaaaggct agccgcacgc gaaattatta ccctgcttgg
     8821 acagaccagc tctactgagt ttgtcgattt ctccggtgga aaactctccc tcatagtgtt
     8881 caaactcgag gaggaatccc cggttgttaa ctacactctg cttcaggcta ccaagaatac
     8941 tcagaatctg gattatagag ccgttgccat atcgcgcgag ggtataacca ttatcccccg
     9001 gggtaacgac caatttaagg taaacgacct ggtttacgtt attgccaacc agaatggtat
     9061 aaaagagatg ttggagtact ccggaaaaaa gaacattgat gttaacaacc tgatgattct
     9121 gggaggaagc cgtatagcca ttcatctagc cactgaactg gagcgttcca tgaacgtaaa
     9181 actgattgag gttgaccccg aaaagagcga aaagttagcc gctcagttca agaatgccct
     9241 tgttattaat ggcgatggac gcgataccga cttgctgatg gaggaagggc ttcagtacat
     9301 ggatgccttt gtggcagtta ccggcaattc ggaaaccaat attctgtcgt gtatcattgc
     9361 taaacgaatg ggggttaaaa aaactattgc cgaaattgaa aatatcaact acataaggtt
     9421 agctgaaagt attggtgttg atagcgtgat taataagaaa ttgattacgg caagccgtat
     9481 attccgctac accatgagca ccgacgtttc caccatcaag tgccttaccg gttccgatgc
     9541 cgaagtactt gaatttattg ttaaaccggg tgccccaata accaaaggta aactcaaaga
     9601 tattggtttc ccaaaagatg ccatagttgg aggtgtggtg cgtggcgata acgtctttat
     9661 tgctaaaggc gatacggaga ttaaacctta cgaccgagta gttgtatttg ccctcccaac
     9721 ggcaatcaac aaggttggta agtactttaa ctaaggtttg gaaggctaac cgtaaaagta
     9781 ctcccccgct gctgctcgct ggttacctta atctgcccgc ctaaatcggt tactagctgc
     9841 ttgcagatta ttaaaccaag cccgctgccc cgctcgttca tcgtaccagg ttgtatcaat
     9901 gattctactg tattaaaaag cctatcggca gtttcccttg gcatccccac cccgtaatcg
     9961 gtaa
//
LOCUS       DAOOWJ010000097         9930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_6786_length_9930_cov_7.654278, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000097 DAOOWJ010000000
VERSION     DAOOWJ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6786_length_9930_cov_7.654278"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLW22_09235"
     CDS             complement(<1..367)
                     /locus_tag="PLW22_09235"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOU74923.1"
                     /translation="MNKISILLFSLTHCCLAIRAGQAQDRRDFGIQAGTTYYYGEFNE
                     VKPLYSPSFAMGVIYRYNINHNYAIRASAIYATVSGSSSSANIIPNPNISFSKNIIGA
                     EAMGEFNFYSINPTSSRKAN"
     gene            complement(367..1146)
                     /locus_tag="PLW22_09240"
     CDS             complement(367..1146)
                     /locus_tag="PLW22_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74924.1"
                     /translation="MRKVIYLIVGLLSGLVLHAQTWKQNRIDVYIGLPVNHYFGDIGN
                     SANTNFISSIKDFRIRALRGGFGGGVGFKVNPFISAQASLNTGFLGNTDDGSYYSSRD
                     YGFSTFYSELTLKGIYYIIPESHQNYYYRIMDFRGGLRHINKPFSLYVFAGAGGLFFS
                     ATPNEKLLSRTPPPGFTKPEVDDSKNFTLVIPVGVGAKYEFYPRFQIGVELGARYVTT
                     DYLDAFSSVYSNHNDMYYTINFNVYYKIPYQKLLKRSFWQF"
     gene            complement(1235..2110)
                     /locus_tag="PLW22_09245"
     CDS             complement(1235..2110)
                     /locus_tag="PLW22_09245"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOU74925.1"
                     /translation="MTIAVYGKRIDAEFAEATRNLFAALEKYRVNVLIYLPLLDYIKS
                     NLSYTPHIGGTFITTQDLKETDVMISLGGDGTFLDSIALVQDMGIPIMGINLGRLGFL
                     ATTSIPEIDNSIDLLMASKYSIEKRSMLQLVSDSKLFDPFPYALNDMVIRNSVAGLLH
                     VNTYINGDYLSTYWADGLIIATPTGSTAYSLSVGGPILTPNLSAFVLSAIAPHHLTVR
                     PLVIPDDSLIELEVSGRDEELIVSLDSRNIRVPQPVKLMLNKAHFNANVICLKGTTFY
                     RTIRSKLLWGMDKRN"
     gene            complement(2115..2780)
                     /locus_tag="PLW22_09250"
     CDS             complement(2115..2780)
                     /locus_tag="PLW22_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU74926.1"
                     /translation="MVAKDMISDVIPPLKTSDTGITALNWMDIFKVSHLPIVNDKEFL
                     GLISESDIYDLNMPEEPLGNHQLSLLRPYVIEDQHVFEVMEVLSRLKLSLVPVLDSSK
                     NYLGVITLMELLHYFTELSALRHKGSVIVLEMNVNDFSLSQICQIVEGNDAKILAAYI
                     TSHPNSTQMELTLKLNVTDVTSIKQTFQRYNYNVLGAYMKQDDESDLLNDRLNYLFKF
                     LDI"
     gene            2834..3550
                     /locus_tag="PLW22_09255"
     CDS             2834..3550
                     /locus_tag="PLW22_09255"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOU74927.1"
                     /translation="MTRLSVNINKIATLRNARGGNIPNVLKAAIDCERFGAEGITVHP
                     RPDERHIRYQDVRDIRPVITTEFNIEGYPSESFIELVLSVTPHQVTLVPDPPNALTSN
                     TGWDTIANKDFLKKVIARFQEHGIRTSIFVNADPLMVKHAADTGTNRIELYTEPYATH
                     FPENPAQAIEPFVKAAEAAMEVGLGLNAGHDLNLDNLNYFARNIPNLLEVSIGHALIC
                     DALYYGLENTIQMYLRQLRF"
     gene            3551..4345
                     /locus_tag="PLW22_09260"
     CDS             3551..4345
                     /locus_tag="PLW22_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU74928.1"
                     /translation="MELFYRQLGQGKPLVILHGLYGLSDNWISIAKNLESSYSLIIPD
                     QRNHGQSPHSPDHTYQHLVNDLLELTNKLKLDSFYLMGHSMGGRVAMLFQSQYPFKVE
                     KLVVVDVAPWSYSPEDEWFRSAYAEHMRIIEALKALRPDEIASRMDADKVLQQNNLNE
                     RLRNFLLKNLKRKSLGFEWQINLNALENNLEHLLLGVEVDKTSALGANVLFIRGENSN
                     YIPAYQAGRLKEIYPNCQVVSIPEAGHWVHAEKPEMFISALKSFLG"
     gene            complement(4342..5067)
                     /locus_tag="PLW22_09265"
     CDS             complement(4342..5067)
                     /locus_tag="PLW22_09265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU74929.1"
                     /translation="MFTDVKLDEILFLDIETVPQYPEYNQMPEEFKKLWDRKAKTLAK
                     PDETPEAIYERAGIYAEFGKIICISVGIIVPSNGNYTFRVKSYYGDSEIDLLKGFAEM
                     VNRFSSKKPNLNLCAHNGKEFDFPYIARRMLVNGIKIPDILNVAGKKPWEVKFLDTME
                     LWKFGDFKHYTSLNLLSYIFGIPSPKDDIDGSEVAKCYYELNDLNRIVIYCEKDVLTV
                     AQLFLRYNGFPIISPENVSRSEV"
     gene            complement(5070..5693)
                     /gene="udk"
                     /locus_tag="PLW22_09270"
     CDS             complement(5070..5693)
                     /gene="udk"
                     /locus_tag="PLW22_09270"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOU74930.1"
                     /translation="MLIIGIAGGTGSGKTTVVNKIIDLLPKGEVVVIPQDSYYRDNSH
                     LPLEQRQEMNFDHPDSIEFELLVQHVKDLKAGKPIEQPIYSYLTCARAPETIKIEPRH
                     VVIIEGILIFTCEELRKLMDIKVFVDADADDRLSRVIARDIVERGRSVNKVLERYEKT
                     VKPMHLQFIEPTKRYADIIVPQGGNNSVAINILTSIIEKTLRSHNLL"
     gene            5780..6775
                     /locus_tag="PLW22_09275"
     CDS             5780..6775
                     /locus_tag="PLW22_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOU74931.1"
                     /translation="MSNSLYPLKFKPILKERIWGGTRLHHSMGKQFPNPEEKYGESWE
                     VSGVEGDVSVVQNGFLAGNTLNELIEVYMGDLMGESLYERFGEEFPLLIKLIDASETL
                     SIQVHPDDETARERHHAYGKTEMWYIIDADRDALIYTGFEKDITREEYIQAVKEGNLE
                     RYLHREKAIPGDVFFIPAGRVHAIGKGVLLAEIQQTSDITYRIFDWNRVDGSGKSREL
                     HTELAVDVIDFARVRNPKSHPVDEVNKTSELSTCKYFTVNRLTFNNAMQRDYNMLDSF
                     VIYLCIDGECSVVYASGSSERLTKGETLLLPADLKDIILRPQGKCSLLEVYVNQA"
     gene            6944..7519
                     /locus_tag="PLW22_09280"
     CDS             6944..7519
                     /locus_tag="PLW22_09280"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOU74932.1"
                     /translation="MDSVIYFIKSTLANEIAASGFPLIYKGEMNHQIMRSFAFMANRK
                     IAEMNVPTATRKRVFHIMIECLQNITKHSDDYDEKEKQIGNGLFVVGKERDSFYIVTG
                     NLVRNDKVPILEQRLDRLNSASPSQLKEMFMQQMVEGELNEKGGAGLGLIDIARKSKR
                     KLYYHFVPYENNRHFFILAVTIPISAEEDDF"
     gene            7698..8603
                     /locus_tag="PLW22_09285"
     CDS             7698..8603
                     /locus_tag="PLW22_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74933.1"
                     /translation="MSKDKIAQNKIETITQYTHRLQNNKVDSKGYKSVETTYDHNGNI
                     VEVVNYKPNGVVASKHLYKYDDRGRKIEYQQFQELPKKGFALAFRQAFTYDSKGNKLT
                     EIGYDGQTTYRVAYSYLPDGKQKDITKYSANNAVEEKWVFEYSTSKVKISIYKPAETL
                     NKIIERTLDSQGRVIDEKNLTPAGKELGRTAFKYGDGDMLLTKVEYYGGELRATYDYV
                     YDEKKQMVEVYMTKPGTSKILYSSYKYDKNGNLLEEKWYEDGASDYSRRNYKVDNQGN
                     INEVDAYYSDYKYRVVYRYSYKFSR"
     gene            8709..>9930
                     /locus_tag="PLW22_09290"
     CDS             8709..>9930
                     /locus_tag="PLW22_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74934.1"
                     /translation="MRHYLKLFLFFALAITSISLFAQEIGRYPLVNFHHRQYNGHSQS
                     WAITQDNRGMIYVANNVGVIEYDGSEWKHISINNALARCLDTDPTGRVWVGGQDEVGY
                     LAADSANSLKYYSLSKLLPERFAKPGLIRQIYHTGQAVYFSTFKALFRINSDLTVNYW
                     EPKTQFHRSYYVDNFIFINQRDYGLTYLYNDSLVKVPGTEILGTQMVYTILPFDYQYL
                     LVGTQTNGFYLLNKQALTTRTLPTDFKTLIPFKTSNDNFFITNRIYSGIMLDEGLWAI
                     GTYSGGVAIINHSGKIVRTISKETGLQDNTAWYLYVDNSKNIWMALNNGISYSPFHSQ
                     LTSWTEQDGISGVIQSAVNFKGTIYYSSNTGVHVKTNSHFERVSGINDLAWGLFIYTD
                     ESGKEHLLSGTANGI"
BASE COUNT         2897 a         2055 c         2158 g         2820 t
ORIGIN      
        1 ggtttgcttt gcggctgctg gttgggttta tggagtaaaa gttaaattcg cccatcgctt
       61 ccgctccaat tatatttttt gaaaagctga tgttggggtt aggaattatg tttgccgatg
      121 aggacgagcc gctaacagtg gcataaattg ccgatgccct tatggcgtag ttgtgattta
      181 tgttataccg gtaaattact cccatggcaa atgaagggct atagagtggc tttacctcgt
      241 taaactcacc gtagtagtag gtagttcctg cctgaatccc aaaatcgcgt cggtcctgtg
      301 cttggccagc tcgtatggct aagcagcaat gggttaagga aaatagtaaa atgcttattt
      361 tattcattaa aattgccaaa atgagcgttt aagtaatttt tggtaaggaa ttttgtagta
      421 aacgttaaag tttatggtat agtacatatc gttgtggttt gagtataccg acgagaatgc
      481 atcgaggtaa tcggtggtta cgtaacgggc tccaagctca acccctatct gaaaacgggg
      541 ataaaactca tactttgctc ctacaccaac aggtattacc agcgtaaaat tctttgaatc
      601 gtcaacctcg ggtttagtaa atcctggtgg tggtgttctg gatagtagct tttcgttggg
      661 tgttgcactg aaaaacaaac caccggcacc tgcaaatacg taaaggctaa atggcttatt
      721 gatatggcgc aaaccacccc taaagtccat aatgcggtag tagtaattct gatggctctc
      781 gggaattatg tagtaaatcc ctttaagggt taactcggaa taaaaagtag aaaacccata
      841 gtctctggag ctgtagtacg agccatcgtc ggtgttacca aggaaacccg tattcaggct
      901 tgcttgtgcc gatatgaatg gattaacctt aaaaccaaca cctccgccaa aacctccgcg
      961 taaggctcta attctaaaat ccttgatgct gctaataaaa ttggtgttag ctgagttgcc
     1021 aatatctcca aaatagtgat taactggcaa tccaatgtaa acatcaatgc gattttgttt
     1081 ccacgtttgg gcatgcaaaa ccaagcccga caataacccc actatcaaat atattacctt
     1141 acgcatggca aatatttatc agcctcaaat atagtaaatg tttaactact atatatttaa
     1201 aacgaattaa atgcaatttt gtttcaacca ccagtcagtt tcttttgtcc atgccccata
     1261 gcaatttgct cctaatggtt ctgtaaaagg ttgttccttt taagcagatg acattggcat
     1321 tgaaatgagc tttattaagc ataagtttaa cgggttgtgg gactctaatg tttcttgaat
     1381 ccagggaaac tatcagctct tcgtctctcc ctgacacttc aagttcaatt aggctgtcgt
     1441 caggaattac aaggggacgg acggttaaat ggtgaggggc aattgccgaa agtacaaacg
     1501 ccgataggtt tggggtaagt attgggccac caacactgag cgagtatgct gttgaacctg
     1561 taggtgttgc aattattaac ccgtcggccc agtatgtgct cagataatcg ccgttgatgt
     1621 aggtattaac atggagtagt ccagcaacgg agttacggat aaccatatcg ttgagagcgt
     1681 atgggaatgg atcaaaaagt ttactgtcgc ttacaagctg gagcatgctt cgcttctcaa
     1741 tggagtactt tgacgccatc agcagatcaa tggaattgtc aatttcaggg attgacgttg
     1801 ttgccaaaaa gcccaaccta cctaagttaa ttcccataat ggggattccc atatcctgaa
     1861 ctagggctat agaatcaaga aaggtaccgt cgccaccaag gctaatcatt acatcggttt
     1921 cctttagatc ctgagtagtg ataaacgtgc cgccgatatg tggtgtgtac gataggtttg
     1981 attttatgta atcgagtagt ggaaggtaaa tcaaaacatt gacccgatat ttttcgagtg
     2041 ctgcaaaaag atttcgggtt gcctcggcaa attccgcatc tattcttttc ccgtatactg
     2101 ctatggtcat ttgcttatat gtccaaaaat ttgaatagat aatttagcct atcgtttagc
     2161 aagtcacttt cgtcatcctg cttcatgtat gctcccagaa cgttatagtt ataacgctga
     2221 aaggtttgct ttattgaggt aacatcggtg acattaagtt tgagcgttag ctccatttgg
     2281 gtggagtttg ggtgcgaggt aatataagcg gcaagaattt ttgcatcgtt tccctccaca
     2341 atctgacaaa tttgcgacaa gctaaaatcg ttaacgttca tctcaagaac aataacgctt
     2401 cccttatgcc tgagggctga aagttccgta aagtaatgca gaagttccat tagggttatt
     2461 acacccaagt agttttttga gctatcgagt actggtacta acgatagctt taggcgcgat
     2521 agtacctcca ttacctcaaa aacatgttgg tcctcaatta catatggtcg caaaagcgaa
     2581 agctgatggt taccaagggg ctcctcgggc atgttgaggt catagatatc gctctctgat
     2641 attaagccaa ggaattcttt atcgtttaca ataggtaggt gcgacacctt aaaaatatcc
     2701 atccagttca aagccgttat tccggtatcg gaggttttga gtggaggaat aacatcggaa
     2761 atcatgtcct tggctaccat atttcttgta aaaaacgatt acttttgtac aaagttaaac
     2821 ttatttgatt gcaatgacac gtttaagtgt aaatataaac aaaattgcca ccttacgcaa
     2881 tgcccggggt ggaaatattc caaatgtgct gaaagcagcc attgattgcg aacgttttgg
     2941 tgccgaaggg attactgttc atcctcgccc cgatgagcgc catatccgtt atcaggatgt
     3001 tcgcgatatt cgcccggtaa ttaccaccga gtttaacatt gaggggtatc cttcggaatc
     3061 gttcatagag ttagttctta gtgttacccc acaccaggtt accctagtac ctgatccacc
     3121 caatgccctt acctccaata ccgggtggga tactattgcc aacaaggact ttcttaaaaa
     3181 agttattgct cgttttcagg agcatggtat cagaacttca atatttgtga atgcggatcc
     3241 cctgatggtt aagcatgctg ccgataccgg aacaaatcgg attgagctat atactgagcc
     3301 atacgctaca catttccctg aaaatcctgc acaggctatt gagccatttg taaaagctgc
     3361 tgaagcagct atggaagttg gccttggatt gaacgcaggc cacgacctta acctcgacaa
     3421 cctgaactac tttgcccgaa atattcccaa cctgctcgaa gtgtccattg ggcatgcact
     3481 tatttgtgat gccctgtact acgggctgga aaataccatt cagatgtacc tccgtcagct
     3541 tcgcttttaa atggaactct tttacaggca gcttggacag ggtaagccat tagtaattct
     3601 acatggcctt tacggcttgt ccgataactg gatttccata gccaaaaacc ttgaaagtag
     3661 ttactctctc ataatccccg atcagcgaaa ccacgggcaa agccctcatt cacctgatca
     3721 tacctaccag catttagtaa atgatttgct tgagctaacc aataagttga aactggatag
     3781 tttctacctg atggggcact caatgggggg gcgtgtggca atgcttttcc aatcgcagta
     3841 tccattcaaa gtggaaaaac tggttgtggt tgatgttgcc ccgtggagct atagccccga
     3901 ggatgagtgg ttccgatcag cgtatgcaga gcacatgcga attattgagg ccctaaaggc
     3961 tttgaggcct gacgaaatag cctcacgtat ggatgccgat aaggtattgc agcaaaacaa
     4021 tctcaacgag aggttgagaa actttctgct aaaaaacctt aaaaggaagt cccttggatt
     4081 tgagtggcaa ataaacctca atgcacttga aaataacctg gaacaccttt tactgggtgt
     4141 tgaggttgac aaaacatcgg cattgggggc caatgtgctg ttcatccgtg gcgagaattc
     4201 aaattatata cctgcctatc aggcaggtag gctaaaggag atttacccta attgccaagt
     4261 tgttagcatt cccgaggctg gtcattgggt gcatgccgaa aagccagaaa tgtttatctc
     4321 ggcattaaag agttttttgg gttaaacctc ggagcggctt acgttttcgg gcgaaataat
     4381 tggaaaaccg ttatacctca ggaaaagctg cgctactgtt aacacatcct tttcacagta
     4441 aatcacaatc cggttcaaat cgttcagttc atagtagcac ttagccactt cgctcccatc
     4501 aatatcatcc ttaggtgagg gaataccaaa gatgtatgat agcaggttca gcgaagtgta
     4561 atgcttgaaa tcgccaaatt tccatagttc catggtatcg aggaatttta cctcccatgg
     4621 ctttttgcca gccacattaa ggatatcggg tattttaata ccgttaacca gcattcggcg
     4681 ggcaatgtat gggaagtcga attccttgcc attatgggcg caaaggttta ggttaggttt
     4741 tttggaacta aaccggttaa ccatttcggc aaagcccttg agtaaatcaa tctcactgtc
     4801 accatagtat gattttactc tgaaagtgta attgccattg gagggcacaa ttatgcccac
     4861 ggaaatacaa ataatctttc caaactcggc ataaattccg gcgcgctcat atattgcttc
     4921 aggcgtttca tcgggcttag ccaatgtttt tgcctttcta tcccaaagct ttttaaattc
     4981 ctcgggcatt tggttatact cagggtattg aggaaccgtt tcaatgtcca ggaaaagaat
     5041 ttcatcgagc ttaacatcgg tgaacatggc tacagcaaat tatgtgaacg gagtgttttt
     5101 tcaataattg atgtaagaat atttatggca accgaattgt tcccaccctg aggtacaatt
     5161 atatcggcat aacgtttagt aggctcaatg aactgcaggt gcatgggctt tacagtcttt
     5221 tcgtaacgct ccagtacctt gttaactgaa cggccacgtt ccacaatatc gcgggctatt
     5281 acacgcgata gcctatcgtc ggcatcggca tcaacaaaaa ccttgatatc cattaacttc
     5341 ctgagttcct cacaggtaaa aatcaggata ccttcaataa ttaccacgtg gcggggttca
     5401 atcttaatgg tttcaggtgc tctggcgcat gttaggtacg agtaaatggg ttgttcaatg
     5461 ggtttcccgg ctttcagatc cttaacatgt tgaaccagca gctcaaactc aatggaatcg
     5521 ggatggtcaa agttcatttc ctgacgttgc tctaagggta agtgtgagtt atcgcggtag
     5581 taggaatcct gagggataac aaccacctct cccttgggaa gcaaatcaat aatcttgttt
     5641 acaactgtag ttttacctga gccggtacca ccggcaatac caattattag catcctatca
     5701 acttttagtg ttacctttgc aaaagtagca atgcaagtta attatttaaa tagttttcag
     5761 caaaaataaa ctgttggcaa tgagcaattc attataccct ttaaagttta aacctatcct
     5821 taaggagcgc atttggggag gtaccaggtt gcaccattcc atgggtaagc aattcccaaa
     5881 ccccgaagaa aaatatggtg agagctggga agtatcggga gttgaaggcg atgtgtcggt
     5941 tgtgcagaat ggttttttgg ctggtaatac cttgaatgaa ctgattgagg tttacatggg
     6001 cgaccttatg ggcgaaagcc tttacgagag gtttggcgag gagttcccct tgctcattaa
     6061 gctcatcgat gcttccgaaa ctctttcaat acaggtacac cccgacgatg aaaccgctcg
     6121 ggaacgtcat catgcctacg gcaaaaccga aatgtggtat atcattgatg ccgataggga
     6181 tgctctcata tacactggat ttgaaaaaga tattacccgg gaagaataca ttcaggcggt
     6241 aaaggagggg aacctggagc gttacctaca tagggagaaa gccatacccg gcgacgtttt
     6301 ctttatcccg gcaggcaggg tgcatgccat tgggaaagga gtactgctgg ccgagattca
     6361 gcaaacctcc gatatcactt acagaatttt cgattggaat agagttgatg gctcaggcaa
     6421 atcccgtgaa ctccataccg agctggctgt ggatgtaatc gatttcgcca gggttcgcaa
     6481 ccccaaatct cacccggtgg atgaggtgaa taaaacaagt gaactttcaa cttgtaagta
     6541 ctttaccgtt aacaggctta cctttaataa cgcaatgcaa cgcgactata acatgctcga
     6601 ctcctttgtt atttaccttt gtattgatgg agaatgtagt gttgtttacg catcgggttc
     6661 cagcgagcgg ctaacaaaag gcgagacact ccttttgcct gccgatctta aggatatcat
     6721 tttacggccg caaggcaaat gctcattgct cgaggtttat gtaaatcagg catgagtttt
     6781 gatgaggtag attataaatt tttaaagagc catttagttc atgaattaaa aaattcattt
     6841 tatcattaaa ttgcattgtt tcataaaaaa ggaagacaag ttcatgagat aaaattttgc
     6901 gggataaaaa ttatttactt atctttaact gatatttttg cgaatggata gcgtcattta
     6961 tttcataaaa tcgaccctag caaatgaaat cgcagcaagt ggttttcccc tgatatataa
     7021 gggcgaaatg aaccaccaaa tcatgcgttc atttgccttt atggcaaata ggaagattgc
     7081 tgaaatgaat gtccccactg caacccgcaa aagggttttc cacatcatga ttgaatgctt
     7141 gcagaacatc accaaacatt ccgacgatta cgatgaaaaa gagaaacaga ttggcaatgg
     7201 tttatttgtg gtggggaagg aacgcgattc cttttacatt gtaaccggca accttgttcg
     7261 gaacgataag gttcccattc tggagcaaag gctcgatagg ttaaatagcg ccagtccatc
     7321 gcaacttaag gagatgttca tgcagcagat ggttgaagga gaacttaatg agaagggtgg
     7381 agccggttta ggattaattg atattgcgcg caaatcgaaa cgcaaactat attaccattt
     7441 tgttccttac gagaataatc gccacttttt catacttgct gttactatcc caatatcagc
     7501 cgaagaggac gatttttaat tttttcatca ttcataagtt gaaaaggtat ggaatttgta
     7561 taaaatcatt tgatttattc taactttgcc gtaacagttt accaagtaaa catttttaca
     7621 tgaaagcttt gagtattttt ttgtttcttg cgggtatttc gtttgggtct ctaactgctc
     7681 aaacaataac cgaactcatg agcaaggata aaattgctca gaacaaaata gaaaccatta
     7741 cccagtacac tcaccgttta cagaacaata aggttgatag taaaggctat aaatcggttg
     7801 agaccacata cgaccacaat ggaaatatag tggaagtggt taactacaaa cccaatggag
     7861 tagttgcctc aaagcatttg tataaatacg acgatagagg acgaaaaatt gagtaccaac
     7921 agttccaaga actccccaaa aaaggatttg ccttggcatt ccgtcaagct tttacctacg
     7981 actctaaagg caataagctt accgaaatag gttacgatgg ccaaacaacc taccgtgtgg
     8041 cttactccta tttgcccgat ggcaagcaaa aggatattac caaatactcg gcaaacaatg
     8101 ccgtggagga gaaatgggtt tttgagtatt caaccagcaa ggttaaaata tcaatttata
     8161 agcccgcaga aactttaaac aaaatcattg agcggactct cgattcgcag gggcgggtaa
     8221 ttgatgaaaa aaaccttacc cctgccggta aggagttagg ccgaacagcc tttaagtatg
     8281 gcgacggcga tatgctatta actaaggtag agtactacgg aggtgagcta cgtgctacct
     8341 acgattatgt ttacgatgaa aaaaagcaga tggttgaggt ttacatgact aaacctggca
     8401 ctagcaagat actttacagc tcatacaagt acgataaaaa cggcaacctg cttgaggaaa
     8461 aatggtacga ggacggcgct tcggactatt cgcgccgcaa ctacaaggtg gataaccagg
     8521 ggaatatcaa tgaggttgat gcctactact ccgactacaa gtaccgcgtt gtttacaggt
     8581 actcctataa gttctccagg taaaacatgc taactgattt atacgcaaag ggtctaaaaa
     8641 gacccttttt tgcttgcttc aatatcaata aattaataac ttgctggtgt ttttaaatca
     8701 aaggggctgt gaggcattat ttaaaactgt ttctgttttt tgcattagca attacaagca
     8761 ttagcctttt tgcacaggaa attggccgct atccgcttgt caacttccat catcggcagt
     8821 ataatggaca ttcgcagagc tgggcaatta cccaggacaa cagggggatg atttacgtgg
     8881 ccaataatgt tggcgtaatt gagtacgatg gctccgagtg gaagcatatt tcaattaaca
     8941 atgcccttgc ccgatgccta gacaccgacc ccactggcag ggtttgggtt ggaggtcagg
     9001 atgaggttgg ataccttgcc gccgattcgg ctaactcact taagtactat tccctttcta
     9061 agctgttgcc cgagcgcttt gccaagcctg gtcttataag gcaaatctac cacaccggtc
     9121 aggcagttta cttttcaact tttaaagctt tatttagaat taacagcgat ttaaccgtta
     9181 actactggga gcccaaaacc cagttccatc gctcatacta tgtggataat ttcattttta
     9241 ttaaccagcg cgattacggc ttaacctacc tttataacga ttcactggta aaagtacccg
     9301 gaaccgaaat tcttggtaca caaatggttt acaccatcct gccattcgat taccagtact
     9361 tactggttgg aacgcaaaca aacggatttt accttcttaa taagcaggca ctaaccactc
     9421 gcaccttacc caccgatttt aaaacgctca tcccgtttaa aacatcaaac gataattttt
     9481 ttatcaccaa caggatttac tctggaataa tgcttgatga gggactttgg gctattggaa
     9541 cctattccgg aggcgttgcc attatcaatc acagcgggaa aattgttaga accatcagca
     9601 aggaaaccgg actacaggac aataccgctt ggtatttata tgtcgataac tccaaaaata
     9661 tttggatggc attaaataat ggtatatcgt actcgccttt tcattcgcag ctaacaagtt
     9721 ggactgagca ggacggaata agcggggtta tacagtcggc tgtaaatttc aagggaacca
     9781 tttactactc ttccaatacg ggtgtccacg ttaaaacaaa ttcgcacttt gaaagggtaa
     9841 gcggcattaa cgatttagca tgggggcttt tcatctacac cgatgaaagc ggcaaggaac
     9901 acttgctctc aggaaccgca aacggaattt
//
LOCUS       DAOOWJ010000098         9612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_7076_length_9612_cov_8.758502, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000098 DAOOWJ010000000
VERSION     DAOOWJ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7076_length_9612_cov_8.758502"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            545..1720
                     /locus_tag="PLW22_09295"
     CDS             545..1720
                     /locus_tag="PLW22_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOU74935.1"
                     /translation="MRFGKIVLTFSVSVFLITARAQEILPGARLMSNYLPLVQGKRVA
                     VVANHTTLVSSKHLVDTLLSVGVTVKKIFSPEHGFRGDIEAGELIGNYTDKVTGLPVI
                     SLYGGSKKPKPADLSDVDVLIFDLQDVGVRFYTYISTMHYVMEACAENNKPLIILDRP
                     NPNGFYVDGPVLDTAYRSFVGMHPVPLVHGMTIGEFAQMINGEGWLKGKIQCNLTVIP
                     CKGYTHSMTTALPVRPSPNLPNHLSILLYPSLGLFEGTVVSVGRGTDYPFQVFGFPNF
                     PDKGFRYIPVEKRGASLNPPYKGKPVYGIDLRDYSVNYFLDRREIILDWLIYSYNNYP
                     EKDKFFNNFFNLLAGTSVLRQQIENGLTPSEIRATWQPALEQFKLLRKKYLLYPDFE"
     gene            1747..2379
                     /locus_tag="PLW22_09300"
     CDS             1747..2379
                     /locus_tag="PLW22_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOU74936.1"
                     /translation="MAKKNTHYVVWEGLKPGIYGSWEECQKQIKGFPGAKYKGFPSML
                     AAQRAYEMGYLAYNSMNPEQEALFNVNENTPKPIYPSLAVDAAWNTATLEMEYQGVDC
                     QTGKLIFHQGPFPDATNNIGEFLAIVHGLAHLKKLNSEIPIYTDSMTAICWVRAKKAR
                     TKLIQTERNKNLFELLERAEKWLRENTWKNPLLKWETQYWGEIPADFGRK"
     gene            2398..3639
                     /locus_tag="PLW22_09305"
     CDS             2398..3639
                     /locus_tag="PLW22_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU74937.1"
                     /translation="MSFESYIAKRFLSGSDSQSLSRPFIRITIASVALSVAVMIVAIA
                     VITGFKREIKQKVVGFGSHIQITNYDSNQSLETNPIPTNLPVIDEVKKVKGVRHIQRF
                     AIKAGIIKTDTDIQGVVLKGIDSDFDWTFFQKNLVDGAIFKITDSTTTTASLISKTLA
                     NLLKLKVGDTYDMFFVQEPPRYRRFTVAGIYDTKMVEFDKMFVLCDIKHIQRLNGWSQ
                     NQATGFEVLVDDIDRVDDLWVEVDDIAGFVFLDDGSRLKVQSIIDKYPNIFDWLNLQD
                     INAAVLIVLMLLVAAINMISGLLIIILEKTSTIGLLKSMGTSNRSVRKIFLIQSAFIM
                     IRGLFIGTAAGLILCFLQKQFGIVKLDEAFYFLSEVPINIVWWHVIVLNVLAFAVGVA
                     MLVVPSMVISRISPDKTLKFE"
     gene            3659..4723
                     /gene="lpxK"
                     /locus_tag="PLW22_09310"
     CDS             3659..4723
                     /gene="lpxK"
                     /locus_tag="PLW22_09310"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOU74938.1"
                     /translation="MQKLRLLLLPFSLLYWFAVKIRNLLFDINVLRSFEFDLPTICVG
                     NLTVGGTGKTPHTDYLISLLKNNYKTALLSRGYGRKTKGFRIVEQSSLAVEVGDEPLQ
                     LKLKHPDVACAVHEDRLNGVVEVMGSFPETEVVVLDDAFQHRWIKAGLNILMVDYNRP
                     VYADFFLPAGNLRDSISEIKRANIVIVSKCPDNLTTHEQKLIAQRIKIKQNQDIYFTS
                     VSYGKPKAVFSNYEDCNFNPNETIFALSGIANPKPFIHQLEKNYKLVGKKTFADHHNF
                     TVKEILSIFEVTSNDVMLVTTEKDAARIRGLNLPSDVKSRIYYLPLEVYFINNNENRF
                     NSQILNYVGENKPNSNLYQE"
     gene            4680..5489
                     /locus_tag="PLW22_09315"
     CDS             4680..5489
                     /locus_tag="PLW22_09315"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU74939.1"
                     /translation="MLEKINQTVTYIKSKVTIEPEVGIILGTGLGGLVREIENQQALE
                     YKDIPNFPVSTVEGHSGRLIFGTLGGKKVVAMQGRFHYYEGYDMKQVTFPVRVMKFLG
                     IKYLFVSNASGGVNPDFEIGDLMIINDHINLMPNPLLGPNINELGPRFPDMHKPYEHD
                     LIALAQKIGAELGFKLQIGCYVGTTGPTFETPKEYQYFRIIGGDTVGMSTVPEVIVAR
                     HMNLPVFAISIITDLGVPGKIVEVSHEEVQKVGAMAEQKMTRIFKEMLTRL"
     gene            complement(6326..6712)
                     /locus_tag="PLW22_09320"
     CDS             complement(6326..6712)
                     /locus_tag="PLW22_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74940.1"
                     /translation="MKKITLIKEKTLKNVPLLVLSTKDKYILSEKHNTITIDENDYIQ
                     ISQMWCKSKKYYSDFFDSQESYFIKVKLNRTILLLAFISTFGGALMYKYYAHNLLFFL
                     PMTLCVIYIFSYLTIFSNRYFFIKKM"
     gene            complement(6833..7051)
                     /locus_tag="PLW22_09325"
     CDS             complement(6833..7051)
                     /locus_tag="PLW22_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74941.1"
                     /translation="METRKLSLQEMEVTEGGSKVLDCISQVTGGMGVLWGTAAALAFG
                     SNPVGWALIGLSAISFAAGVASNPGACD"
     gene            complement(7185..9368)
                     /locus_tag="PLW22_09330"
     CDS             complement(7185..9368)
                     /locus_tag="PLW22_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOU74942.1"
                     /translation="MKIDKRIAKTFVLQKNQSDCGVACLSSIIKFHGGVPNLEKLRIA
                     SGTSKQGTTLLGLYQVANSIGFNAEGMQADVAFLREINQPVILHVVVNGSMLHYVVCY
                     KVSQNSFLIGDPALGLKWMNEAELLDIWKSGKLLTLVPDEKRFKKEKTERSLKWDWFK
                     KLLREDLSLLTTILLMGIAVAVLGLATAVFAQVFIDRLIPSGNATRIYAAIGLVSILL
                     TARLLIGYYRQQLMLRQGVDINVRITGSFFADLLLLPMGFFTSRKIGDMVARLNDIGR
                     IQQTVSFFFGELLINALVVVVSLAAVFLYNIWVGLILLMGVPLFVWVAYRYNRAIING
                     QRDAMMAYSLNESSYINTIGNIQPIKSFRKETFFTRVGLLTFRFFQDKVYHIGSIGIT
                     VQLLTGMIGLAITIGVIILSAIQLQSGQMSTGVFMAVFSLSGTILPALASIAFANVQL
                     QGARIAFERMYEFSSMDKECLKADEVEKERTEKFQELCLQDVSFRYPGRGLLLENINF
                     SVRKGEHVTIFGDNGTGKSTLISLIQRFYSPESGKLLINGKDAETYSIQSYRGLIGVV
                     PQDVTLTNATLLANITLSDSEKEALEAVETLNRYGLVPFFEKFPQGFHTMLGEGGVQI
                     SGGQKQLVGLARALVCKPQLLLLDELTAHMDRSTENFVLELLAQLKTSMGILSITHSI
                     RNASVSDRIIVLSGSGQVEIAGNHNELLKSQNLYSAAWQSFTKTH"
     gene            complement(9371..>9612)
                     /locus_tag="PLW22_09335"
     CDS             complement(9371..>9612)
                     /locus_tag="PLW22_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74943.1"
                     /translation="NILLLSHQSTNELLRIQANYNLDNYANFEMLKMDELHTEEPFLS
                     ASNPSIFVFDHNGRLMLQKKGYTRPELLIKQLLHK"
BASE COUNT         2789 a         1995 c         2039 g         2789 t
ORIGIN      
        1 gcatcgaaac agaaaaatca ttacaaagag cgattgccga aaaaccaatc aaaaggtgat
       61 gaagggagat agagatgctt cggctagctc agcatgacaa gagtgcagaa aggaagatag
      121 ggtttttgta atcccaagcg tagtcgaaga atctcattaa atagtgaatg aaggtataat
      181 gagatgcttc ggcaaagtct atccctataa atcaggactc agcaagacag cataatgttt
      241 ctcttgcgcg caacatgaca aataaactta actaagcaac attgatagat aatccatcat
      301 tttaaaacga gagtaaaaat caatgaaatc taaaaaattc actttgagat atttcaccat
      361 tttatccttt atcctttaac ctttatcctt aattacgttt taccggttct gaatttttca
      421 gctaaactca gcctcccgta gagttgttta tgcagagcat ggttctcccc ctttcttttt
      481 tctgtataaa ttattttcta ccttagcaaa accataattg gcgcatattt tgcaaaagga
      541 aggtatgagg tttggtaaaa tagtattaac cttttcggtt tcagtatttc tgataaccgc
      601 aagggcacag gaaattttgc ctggtgctcg tttgatgagc aactaccttc cgttggttca
      661 gggcaaacgg gtggctgttg tagccaacca tactacgctg gttagttcaa agcacctggt
      721 cgatactctg ctttcagttg gagtaaccgt aaaaaaaata tttagcccgg agcatggttt
      781 tcggggcgat attgaggctg gcgagcttat tggaaactat accgataagg ttacgggatt
      841 acctgtaatt tcgctgtatg gcggaagtaa aaaacccaaa ccagccgacc tgagcgatgt
      901 tgatgttcta atttttgatt tgcaggatgt tggagtaagg ttttacacct acatctcaac
      961 catgcattac gtaatggagg cctgcgcgga aaataacaag ccgttgataa tactcgatag
     1021 gccaaaccct aacggttttt acgttgatgg gccagtactg gacaccgctt accgctcgtt
     1081 tgtgggaatg catcctgtac ccctggttca cggtatgacc attggtgagt ttgcccaaat
     1141 gataaacggt gagggctggt taaaggggaa aattcagtgt aacctcacag ttattccctg
     1201 taagggttac acccacagca tgaccactgc cctacccgtt cgcccctccc ctaacctccc
     1261 caatcacctt tccattctgc tttacccttc gcttgggctg tttgagggga cggtggtaag
     1321 cgtaggccgt ggaaccgatt accccttcca ggtttttggt ttccctaatt tccccgataa
     1381 gggttttagg tacataccgg ttgagaaacg tggggcaagc ttgaatccgc cctacaaggg
     1441 caaaccggtt tacggtatcg acctaaggga ctactcggta aactactttc tggaccgccg
     1501 cgagataatt ctcgattggc taatatactc ctacaacaac taccctgaaa aagataaatt
     1561 cttcaataac ttcttcaacc tgcttgccgg aactagcgtg ctgcgccagc aaatagaaaa
     1621 cgggttaacc ccttcggaaa ttagagccac ctggcagcca gcgcttgagc agttcaaact
     1681 gcttcgaaaa aagtacctgc tttaccccga ttttgaataa ctttgacact tgattactgt
     1741 aaaagaatgg caaagaaaaa tacacactat gtggtttggg aagggctaaa acctggcatt
     1801 tatgggagtt gggaggagtg tcaaaagcaa ataaagggct ttccgggtgc aaagtataaa
     1861 ggatttccaa gcatgcttgc cgcacagcgt gcttatgaaa tgggttactt ggcatacaat
     1921 agcatgaacc ccgagcagga agcactattt aatgttaacg agaatacccc aaaaccaata
     1981 tacccctcat tggctgttga tgcagcctgg aatactgcta ctcttgaaat ggaataccaa
     2041 ggggtagatt gtcaaaccgg caagctgatt ttccatcaag gacctttccc cgacgccacc
     2101 aataacatag gcgagttcct tgccattgtc catgggttag cgcatctgaa aaagctgaat
     2161 agcgaaatac ccatttacac cgactccatg actgccatat gttgggttag ggcaaagaaa
     2221 gcacgtacca aacttattca aacggaaagg aataagaatc tctttgaact actagagcgg
     2281 gccgaaaaat ggttaaggga aaacacatgg aaaaaccctt tgcttaaatg ggaaacccag
     2341 tactggggcg aaatccctgc cgattttggc agaaaataaa caaaatgcca actacaagtg
     2401 agttttgaaa gctacatagc aaaacgattc ctaagcggtt ccgatagcca aagcctatcg
     2461 aggccattta tccgtattac cattgcctcg gtagccctga gcgtggctgt tatgattgtg
     2521 gccatagccg ttattaccgg ctttaaaagg gagattaaac aaaaggttgt gggttttgga
     2581 agccatattc aaataacaaa ctacgacagt aaccaatcgc ttgaaaccaa tcccatacca
     2641 acaaaccttc cggtaattga tgaggtaaaa aaagtaaaag gggttaggca tattcagcgc
     2701 tttgccatta aggcaggtat cataaagacc gacaccgata ttcaaggagt tgtcctaaag
     2761 gggatagatt ccgatttcga ttggactttt ttccaaaaga acctagttga tggggcgatt
     2821 ttcaaaatta ccgactcaac cacaaccact gcatcgctta tttccaaaac gctggcaaac
     2881 cttttaaagt taaaggtagg cgatacctac gatatgttct ttgttcaaga accaccccgt
     2941 tatagaaggt ttacggttgc tggtatttac gataccaaaa tggttgagtt cgataaaatg
     3001 tttgtgcttt gcgacattaa gcacatccag aggctcaacg ggtggagcca gaaccaggcc
     3061 acaggatttg aggttttggt tgatgatatc gatagggttg atgacctatg ggttgaggtt
     3121 gatgatattg caggctttgt tttcctcgac gatgggtcaa ggctaaaggt tcaaagcatt
     3181 atcgataagt acccaaatat ttttgactgg ttgaacctgc aggatattaa cgctgccgtt
     3241 ctaattgttt taatgttgct agtggcagca attaacatga tttcgggctt actcattatt
     3301 attctggaaa aaacatctac cattggcctt ttgaaatcga tgggaacatc gaaccggagc
     3361 gttagaaaga tatttttgat acagtcggcg ttcattatga tacgcggact ttttattgga
     3421 accgcagcgg gtttgatact ttgttttttg caaaagcagt ttggtatagt taagctcgat
     3481 gaggcatttt acttcctatc cgaagtgccc atcaacatag tttggtggca tgtaattgtt
     3541 ttgaatgttt tagcctttgc cgttggagtg gctatgctgg tggtgccatc gatggtaatt
     3601 tcgcggatta gccccgataa aacacttaaa tttgagtagt aactttatca gtaaaaccat
     3661 gcaaaagctt cgtcttttac ttttgccctt ttcgttgctt tactggtttg cagtaaagat
     3721 tcggaacctc ctttttgata tcaatgttct gcgcagcttt gagttcgacc ttccaaccat
     3781 ttgtgttggt aacttaaccg tggggggaac aggcaaaacc ccgcataccg attaccttat
     3841 tagtttactg aaaaacaact ataaaacggc actgctaagc cgtgggtatg gtcgtaaaac
     3901 caaaggtttc agaattgttg agcaatcgtc gctggccgtt gaggtaggcg atgaacccct
     3961 tcagcttaag ctcaaacacc ccgatgttgc ctgtgccgtg catgaggaca ggctaaacgg
     4021 cgtggtggag gtaatgggct cgttccctga gaccgaggtt gttgttctgg acgatgcctt
     4081 ccaacaccgt tggataaaag cagggcttaa catcttaatg gtggattaca accgaccggt
     4141 ttacgccgat ttctttttgc ctgccgggaa tctccgcgac agcatctcgg agattaagcg
     4201 ggccaatatt gttatagtga gcaaatgtcc cgataacctt acaacccatg agcaaaagtt
     4261 aatagcccaa aggattaaaa tcaagcaaaa ccaggatatt tattttacct cagtgagcta
     4321 tggtaagcca aaggctgttt ttagcaatta cgaagactgt aattttaatc caaatgaaac
     4381 catcttcgct ttatcgggaa tagcaaatcc caagccattt atccatcaac tcgaaaaaaa
     4441 ttataaattg gtaggaaaga agacctttgc cgaccatcat aattttacgg ttaaggaaat
     4501 tctatctatt tttgaggtca cctcaaacga tgtaatgctg gtaaccaccg aaaaggatgc
     4561 agctcgtatt cgtgggctaa acctgccatc ggatgtaaaa agtaggattt actacttgcc
     4621 gcttgaggtt tactttatca ataacaacga aaataggttt aacagtcaaa tactcaatta
     4681 tgttggagaa aataaaccaa acagtaacct atatcaagag taaggttacc attgagcccg
     4741 aagtggggat tatacttgga accggtttgg gtggcttagt tcgtgaaatt gaaaatcaac
     4801 aggcattgga gtataaggat atacctaact ttcctgtttc cactgttgaa ggacactcgg
     4861 gacgattaat atttggcacc cttggcggta aaaaggttgt agccatgcag ggccgcttcc
     4921 actactacga gggttacgat atgaaacagg taaccttccc ggttagggtg atgaagttcc
     4981 ttggcattaa gtatcttttt gtatcaaatg ccagcggggg cgtaaacccc gactttgaga
     5041 ttggcgattt gatgataatt aacgaccata tcaacctaat gcccaatccg ctcttaggtc
     5101 ccaatatcaa tgagctcgga ccccgtttcc ccgatatgca taaaccctac gaacacgatt
     5161 tgattgcctt agcccaaaag attggggctg aactgggctt caaattgcag attggatgct
     5221 acgtgggaac tactggccca acctttgaaa cacctaagga atatcagtat ttccgtatta
     5281 ttggcggtga cacagtgggt atgtcaaccg ttccggaggt tattgttgcc cgtcacatga
     5341 acctgcccgt gtttgccata tccattatta ccgatttggg tgttccaggc aaaattgttg
     5401 aggtgtcgca cgaggaggtt caaaaggtag gagccatggc cgaacaaaag atgacaagaa
     5461 ttttcaagga gatgctcaca aggctataat ccttaatgct attcgcaggg agaggctacc
     5521 tgtttagggt ggcctttttt attaagttga tggttgatag ttgatggttg atggttgacg
     5581 gttgttcgtg agtgtattgt catgttgaga gctagcgaaa aatcctaata catagatttt
     5641 cattgcaaat gcaaaaaatc tacctatgat taaaaacggc aatagcagta ttctaaaact
     5701 cttcctgcaa atgaaaagga gagaatagtt caggggactt tggagttcag ctgaactaat
     5761 gagctggcaa ttgtttatgc cttcataatt ctcatagcgg ataggggtta aaaataatgc
     5821 aaggtttata ggcctaattc actagatctt gcaaatattt ttccgaaaac cacttgctat
     5881 attattagta gtcaaactgt cattatatta ataagggtca actagttagg gctaacggct
     5941 gggggttggt aaagtgcagg agtttgaaac gatttcctat cccacgctac aaagcttcaa
     6001 aaaaccaata aatctttcta aactactaaa tacctgcatt ttattaaccc cttgttatgg
     6061 gcaggttttt taatttgaga tgaaagagtg tttcttacgt atagaaatta gtgtaatggt
     6121 agataatatt tagattaaag aatccaccct ctaagaaggt ggacttaata ttgactccta
     6181 caaggagcca atattaagtc cacggttgtt cttctaaacg ttcttggtct tttggatatt
     6241 tcacgtactt tttgctcttg tcttcatcaa ggcctattgt gtctatacaa taccctgtac
     6301 atttagttat tacaggattg tctttttaca tcttttttat gaaaaagtat ctgttgctaa
     6361 atatcgttaa ataggaaaat atataaataa cgcacaatgt cataggcaag aaaaaaagca
     6421 agttatgtgc atagtactta tacattagag cacccccaaa ggttgaaata aaagctaaca
     6481 acaatattgt tcgatttaat tttactttta taaaataact ttcctgtgaa tcaaaaaaat
     6541 cagagtagta tttttttgac ttacaccaca tttggcttat ttgaatataa tcattttcat
     6601 caattgtgat ggtattatgc ttttctgaca aaatatattt gtctttagta gacaaaacca
     6661 ataaaggtac attctttaag gttttttctt ttattaaagt tatctttttc atgatttttg
     6721 ctcattttta aagaaggttt gctcttaatt atttgacaaa ccttcttttg ggtaatactt
     6781 gctgagttca attttgctga ggataatttg atttgtttat cagcctcgtt gactaatcac
     6841 aagcacctgg gttactagca acacctgcgg caaaactaat tgcggaaagt ccgataagtg
     6901 cccagccaac agggtttgac ccaaatgcta aagctgcggc agtaccccat aagactccca
     6961 taccaccagt aacttgtgag atgcaatcta acactttgct acccccttca gtcacttcca
     7021 tttcctgcaa agataatttt cttgtttcca ttttactaat tttttaaggt taataataaa
     7081 gttaacttac aagtgctaaa ttattgaact gaattctcat caccaaagaa aaatgtgaca
     7141 aattccgtga acggtcaaaa atagcgtccg agcgggattt tttttcagtg cgtttttgta
     7201 aaagactgcc aagcagcact atacagattt tggcttttca acagctcatt atggtttccg
     7261 gctatttcaa cctgaccact acccgacagc acaataatcc tgtcggaaac ggaagcgttt
     7321 ctaatactgt gcgtaatgct aagaatgccc atgctggttt tgagttgggc tagcagctcc
     7381 aaaacaaagt tctctgtact tctgtccatg tgtgcggtta gctcatcgag cagcagcaat
     7441 tgaggtttac aaactaatgc acgagccaac cctaccagct gcttttgccc tccagaaatt
     7501 tgaacacctc cctcgccgag catggtatga aaaccctgtg gaaatttctc aaagaatggg
     7561 actaaaccat acctgttcaa tgtttcaacg gcttctaagg cttccttttc actatccgaa
     7621 agggttatgt ttgcgagtaa agttgcgttt gttagcgtta catcttgtgg gacaactcct
     7681 ataaggcctc tataagattg gattgaatag gtttcagcat cctttccgtt aatcagtaat
     7741 ttacccgatt cagggctgta gaaccgctgt ataagagaaa ttaatgtgct tttacccgtg
     7801 ccgttatcgc caaaaatagt aacgtgctcc cctttacgga cggaaaagtt tatgttttcg
     7861 agtaacagcc ctctgcctgg atacctaaat gagacatcct gtaggcacag ttcttgaaat
     7921 ttttcagtgc gctctttttc aacttcgtca gcctttaagc actccttatc catgctggag
     7981 aactcgtaca tccgctcaaa ggcaattcgt gcgccctgta gctgcacgtt ggcaaaggca
     8041 atggaagcta aagctggaag gatggtaccg cttagactaa aaacagccat aaagacaccc
     8101 gttgacattt gccccgattg cagctgaatg gccgataata tgattacacc gatagtaata
     8161 gccagtccga tcatgcccgt aagcagctgc acggttatac caatacttcc gatatgatat
     8221 actttgtcct gaaaaaaacg aaaggttagc aatcccaccc ttgtaaaaaa ggtttccttt
     8281 ctaaagcttt taatgggctg gatattgcca atggtgttga tgtagctgct ctcgttgagg
     8341 gagtatgcca tcatggcatc cctttgaccg ttaataatgg ctctgttgta gcggtatgct
     8401 acccatacaa atagcggcac gcccataagc agaatcaaac caacccatat gttgtaaaga
     8461 aaaactgcgg ctagtgaaac aacaacaaca agggcattaa ttagtagctc tccgaaaaag
     8521 aaagaaaccg tttgctgtat gcgccctatg tcgtttagcc ttgccaccat gtcgccaatt
     8581 ttacggcttg tgaagaagcc catggggagc aggagcaaat cggcaaagaa gctacctgta
     8641 attcttacgt taatgtcaac accctgtcgg agcatgagct gctgcctgta gtagccaatt
     8701 agcagccttg ctgtcagtaa tattgaaacc aaaccaatag ctgcataaat gcgagtggca
     8761 ttaccagatg gtataagcct gtcgatgaat acctgagcaa aaacggctgt agctaacccc
     8821 agcactgcaa cagcaattcc cattagaagg atggttgtta aaaggcttaa atcctccctc
     8881 aacaactttt taaaccaatc ccatttcagg ctgcgctcag ttttctcttt tttaaacctt
     8941 ttctcgtcag gtactagcgt tagcaatttt ccgcttttcc agatgtccaa cagttcagct
     9001 tcattcatcc actttaagcc aagggccggg tcgccaatta ggaaactatt ttgcgatact
     9061 ttgtagcaca ccacgtagtg tagcattgag ccattcacaa caacatgcag aataacaggc
     9121 tggttaattt ctctgaggaa tgccacgtca gcctgcattc cctcggcgtt aaaccctatg
     9181 ctgttggcta cttggtataa gcctagtagc gtggtgccct gcttgcttgt accgctggca
     9241 attctcaatt tttccaaatt tggaacgcct ccgtggaact ttatgatgct gctcaggcag
     9301 gcgaccccgc aatcggactg gtttttttgt agaacaaagg ttttcgctat tcttttgtca
     9361 attttcatgg ctatttatga agaagctgtt ttattagtag ctcggggcgg gtataaccct
     9421 ttttctgtaa cattaacctg ccgttatggt caaatacaaa aatggatggg ttggatgcgc
     9481 taaggaatgg ctcttctgta tgcagctcat ccatttttag catttcaaag ttggcatagt
     9541 tgtctaggtt gtaattcgcc tgtatgcgaa gcagctcgtt tgtgctctga tgggaaagca
     9601 acagtatgtt gt
//
LOCUS       DAOOWJ010000099         9289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_7364_length_9289_cov_6.167966, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000099 DAOOWJ010000000
VERSION     DAOOWJ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7364_length_9289_cov_6.167966"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1596
                     /locus_tag="PLW22_09340"
     CDS             253..1596
                     /locus_tag="PLW22_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74944.1"
                     /translation="MNRKKSNLLLIAVALLGLMVTSCDKNDDDTPKGTKKTVSGEISQ
                     NTLWEAKDTIILDGYVYVKSGATLTIEPGTLIKGLSDSKACLIVERGARIVANGTAEK
                     PIIFTSDKPVGERKPGDWAGIIICGKAPINQTGGEAEIEGGTGAVYGGTDPNDNSGIL
                     RYVRIEFAGYEVSNGNEINGLTMGGVGRGTTIEYVQVSFGRDDSFEWFGGTVNTKYLI
                     SYRGGDDDFDTDNGFSGNVQFGLILRGPEEADKSDASNGFESDNDANGSSNSPLTSAK
                     FSNITILGPYATKTQANVNPNHKFGLRLRRNTRLNLYNSIIAGFNTGISLEGPSMELY
                     NAASGPEIKNCLMAGMVKNFNAKTDGTSLTQAQYETMFNAGNNTLYAENTQLQLTNAW
                     NWGNVNAMPQSGSPALTGASFTGLTGFETVTFRGAFGTQNWAQGWTNWDPVNTNY"
     gene            1697..2155
                     /locus_tag="PLW22_09345"
     CDS             1697..2155
                     /locus_tag="PLW22_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOU74945.1"
                     /translation="MKLFLTLLISLVSLVSFAQTNVVEREGKYYDSNGKLYSGRYTSY
                     YDNGNIKVEALITNGLPDGEMKLYYPTGKIQEIRTYSLGEKNGRWEKWNEMGIKVSEA
                     NFVKGKKHGKWYVWDDNGTLRYDMTYTDGEKSGTWIIYDENGKETSRKEY"
     gene            2244..3041
                     /locus_tag="PLW22_09350"
     CDS             2244..3041
                     /locus_tag="PLW22_09350"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU74946.1"
                     /translation="MISFKNRLVWITGASSGIGKELALQLAEEGAHLIISSNDAQELS
                     HVADECREFTSFCQEYPFDLSSPVEVKNTAETIINTYGQIYLLINNGGISQRSLVKDT
                     PVEIDRKIMEIDYFSYVILTKTVLPGMIEAGEGFIAATSSISGKFGFPLRSSYAAAKH
                     AIQGFFETLRAEMKPHNISVTIAYPGRIQTNISLRAIDQNGKEHGKMDPGQQNGLSAK
                     ECARQYIKAIKKRKPEVFIGKSEIFMVHIKRLFPSIFFRIVTKIKPT"
     gene            3047..4123
                     /locus_tag="PLW22_09355"
     CDS             3047..4123
                     /locus_tag="PLW22_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU74947.1"
                     /translation="MSKIIYGIQQVGIGVSNLSEAWKWYKEHLGFDIRIFEDNTVAEL
                     MLPYTGGQPQQRHAALALNMQGGGGLEIWQYKGRTPLMPEFQVLLGDKGIFAAKIKCP
                     NVKKAYEYFERMGVPVSQLAVNPNGDEHFFIKDPFGNFFEIYSHSYIFKDEKKPVSGV
                     GGAIIGVSDIENALKVYRDILGYDTVLYDKSGNFDDVAELPGGKGSFRRMLLTHSENR
                     KGSFSNFFGKSEIELVQALDREPNKIFKNRFWGDLGFIHLCFDMKNMNDLENECVAKS
                     FPFTVNSNVKHNEKGSFDMGEAAGHFSYIEDPDGTLIEFVETHRLPLIKPLGIQLNMD
                     KRDPHKPIPNWITKLLSLKRVKNL"
     gene            4736..6907
                     /locus_tag="PLW22_09360"
     CDS             4736..6907
                     /locus_tag="PLW22_09360"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOU74948.1"
                     /translation="MRINSILSIFLLVQSAYSQTINHWETAIFASDTWRYRVNQTTTP
                     TGWTLHSFEDSQWPQGPGGFGYGDNDDATVVPSCISVSIRTTFIVSDVDEMAMAVLSV
                     DYDDAFIAWINGVEVARSAGLQGVNVNWDTPSSENREAMMYTGGNPTDFLIKMDVLSS
                     VLRSGLNVLAIEVHNTGINSSDLSIIPFLSFGITVNNTYYRPTPEWFNPPVTSLSSNL
                     PIVIIDTQGQAIQSDNEIVATMKVVDKQSPPNSIADNDFTYNGTIHLEYRGQSSLWND
                     WPKKSYNVELINALGENIDSALMGMPPGNDWALYGPYNDKSLIRNALAYEIGRRMGRY
                     APRTAFCELILNEQYAGLYLLIEKVRRDKNRVNIAKLTQNDISGDDLTGGYIVKIDKG
                     DFDEFGWASPYTHNGKPINFLWHYPKPENILPVQKNYIRNYITTFENTLNSSYWNDPF
                     LGYYRYVNMASAVDYFLVNELSKNIDAYRISTFLHKDKDSKGGKLTFGPMWDYDLTFG
                     NANYYSGDSPQGWVFKSISTDDYYQPPFWWDKFTTDDKFNNMARCRWEELKSSGLLTR
                     TGVHAIIDSMTTLIQDARIRNYNTFNSLGVWVWPNAFVGSTYEQEVNYLKNFVSSRLL
                     WLDANLPGVCKDEPNPIMISAIPNPFNEYFDLRVILPEVGQITLKLTSISGSVVWQMS
                     ENATLTSFVYRYQAQDLQKGIYILTAYLNGKYLGAVKVIKG"
     gene            complement(6916..7407)
                     /locus_tag="PLW22_09365"
     CDS             complement(6916..7407)
                     /locus_tag="PLW22_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOU74949.1"
                     /translation="MIKGENHKALTPQKAMERLQYLCARQEKCTHDLVQKLKQWGFAQ
                     AEIDKIVGKLSSDGYVDDNRFARLYVREKSRINKWGPIKLRAMLVSKGIAKDIIDQAL
                     NELRYAENPVNLFDLLNKKMPQVKAKNRFDLRNKLIRFGASRGFALDEVIDTVNRLLG
                     AEE"
     gene            complement(7404..8294)
                     /gene="prmC"
                     /locus_tag="PLW22_09370"
     CDS             complement(7404..8294)
                     /gene="prmC"
                     /locus_tag="PLW22_09370"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOU74950.1"
                     /translation="MFYTKLSNFMEHITLSGLLNHVSRSLSGSFNENELHTIKKYIAE
                     AVLHVPYHQVFINKHHEVKHEDAQEVERIISELISGKPLQYAIGLAYFSDLVLKVTPS
                     VLIPRPETEELVSLIVKENRIVAPRILDVGTGSGCIALALAKHIPDASVAAIDISTDA
                     LDVARQNAKSNNLNVNFFQGDILQPTVLDGYTFDIIVSNPPYIRDSEKVLMNRNVLDF
                     EPHIALFVPDSDPLKFYRAIALHSVRRLETMGWLWFEINEALGSEIVSLLKSLGFGNV
                     LLIDDFRGKPRFVKAQKCNP"
     gene            8308..>9289
                     /gene="ribD"
                     /locus_tag="PLW22_09375"
     CDS             8308..>9289
                     /gene="ribD"
                     /locus_tag="PLW22_09375"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOU74951.1"
                     /translation="MENADVHRKYMARCLELALGGQGNVAPNPMVGAVIVHNNQIIGE
                     GFHAMYGQAHAEVNAINSVQNKELLKESTLYVSLEPCSHYGKTPPCTDRIVEYEIPRV
                     VIATTDPNPKVAGRGIEILRQNGKEVIVGVLEKEAIELNRRFITFHTKNRPYVILKWA
                     QTMDGFIDKKREPNEPVGSNWITNELARSLVHRWRSEEQAVLVGTNTVEKDNPQLNVR
                     NWSGRSPIRVVVDRKLRLPLNSNVFDGSQPTLLVTGNNSSALARKTEFSGIDNLEIIT
                     IDFVKGVEAQILKELAEREILSVIIEGGAMLLNSFIQKNLWDEARMFVGNK"
BASE COUNT         2805 a         1990 c         1955 g         2539 t
ORIGIN      
        1 ctgctgaggt tgaggtgaaa aaaagttcaa aggaggtaaa gattgagtta aacacagttg
       61 agtagaattt agaagttaac ataaaatact aaaccagagt cggatgccct aatccggctc
      121 ttttttttca ccttaacctt aatgtaacaa actgctaatg taaagctaag ctcaccttaa
      181 cgttaaagtt cgatttgctg gatacttttg gattgtgaaa aagaaataaa ctaacctcta
      241 aatactaatt gtatgaatcg gaaaaaaagt aatttgttat taattgcagt ggccctatta
      301 ggcttaatgg taacatcgtg cgataagaac gacgacgata cccccaaagg aaccaaaaaa
      361 accgtatcgg gtgaaatttc acaaaacacc ctttgggaag caaaggacac cattattctc
      421 gatggctatg tgtatgttaa atcaggagca accttaacca ttgaaccagg tacactgata
      481 aaagggttaa gcgactcaaa ggcatgcctt attgtggaac gtggagctag aattgtagca
      541 aacggaactg ctgaaaaacc tattattttc accagcgata agccagtagg agaacgtaaa
      601 cccggcgact gggcagggat aattatttgc ggtaaagccc ctattaacca aaccgggggt
      661 gaagcagaaa ttgaaggtgg aaccggtgct gtatacggtg gaacagaccc taacgataac
      721 tcaggcatac taaggtatgt gcgaattgag tttgctggct atgaagtttc caatggtaac
      781 gaaattaatg gtttaaccat gggcggcgta ggccgaggaa ctaccattga gtatgtacag
      841 gtttcattcg gccgcgatga ttcattcgaa tggtttggcg gaacagtaaa tactaaatac
      901 ctgatttcct atcgcggtgg cgacgacgac tttgataccg ataatggttt cagcggtaat
      961 gtgcagtttg gtttaatcct tcgtggtccg gaagaagccg ataagagcga tgcttccaac
     1021 ggttttgaat cggataatga tgctaacggt tcatcaaata gccctcttac ttcagcaaag
     1081 tttagcaaca ttacaattct tggcccttat gccactaaaa ctcaagccaa cgtaaacccc
     1141 aaccataaat tcggcttaag gttacgccga aatacccgtt tgaacctata caactccatt
     1201 atagctggtt ttaatactgg aatttctcta gaaggaccat cgatggaact ttacaatgct
     1261 gccagtggtc ccgaaatcaa gaattgttta atggctggta tggtaaagaa ctttaatgct
     1321 aaaaccgatg gaacaagctt aacacaggcg caatacgaaa ccatgtttaa tgcaggtaac
     1381 aatacccttt atgctgaaaa cacccaactt caacttacga atgcctggaa ctggggtaat
     1441 gtaaacgcca tgccacaaag cggttcccct gcattaactg gtgcttcgtt taccggactc
     1501 acaggatttg agacagttac tttccgcggt gcttttggaa cccaaaactg ggctcaggga
     1561 tggaccaact gggatcctgt taataccaat tactagtgct tttatttcat aaaatcaata
     1621 aacttcccga tattcgggaa gtttttttgc accttttttg aagtaattat cgtaaaataa
     1681 gtctaaaatg cacattatga aattattttt aacgttactc atctcattag tttcgctggt
     1741 tagctttgcc caaaccaatg ttgttgaacg cgagggtaaa tattatgata gtaatgggaa
     1801 attgtattca ggtcgctaca cctcctatta tgacaatgga aacattaaag ttgaagcctt
     1861 aatcaccaac ggactacccg atggcgaaat gaaactatac tacccaaccg gaaaaattca
     1921 ggaaattagg acctacagcc ttggtgaaaa gaatggccga tgggagaaat ggaacgaaat
     1981 gggcataaag gtttccgaag caaactttgt gaagggtaaa aaacatggga aatggtatgt
     2041 ttgggacgat aacggcactc tccgatacga tatgacctat accgatggcg aaaaaagcgg
     2101 tacctggatc atttatgacg aaaacgggaa ggaaacctca cggaaagaat actaggttct
     2161 cagaattaac tttataaaaa agtgttacat ttgacatcaa aatttttggg catacacatg
     2221 ctcaaagtat aatttgatgt ttgatgattt catttaaaaa ccgacttgtt tggataactg
     2281 gtgcctcatc gggcatagga aaggaattgg ctttacagct cgctgaggaa ggggctcatc
     2341 tgattatctc gtcgaacgat gcccaggagt taagccatgt ggccgatgag tgccgtgagt
     2401 tcacctcgtt ctgccaggaa tatccttttg acctttccag tcccgtagag gtaaaaaaca
     2461 ctgctgaaac cattattaat acttatggtc agatatacct gctaatcaat aacggtggca
     2521 taagccaacg ttcacttgtg aaagacacac ctgtagaaat agaccgtaaa attatggaga
     2581 tagattactt ctcctacgta atactcacta aaacggttct tccaggaatg attgaggcag
     2641 gtgagggttt tatagcagcc acaagcagca tttcgggtaa attcggtttt ccattgaggt
     2701 cctcgtatgc agcagcaaag cacgccattc agggtttctt tgaaacccta cgtgcggaaa
     2761 tgaaacccca caacatttca gttaccattg cctaccccgg tcgtattcaa acaaacatct
     2821 cgctcagagc tattgatcag aatggaaaag aacatggcaa aatggatccg gggcagcaga
     2881 acgggttaag tgcaaaggaa tgtgcccgac agtacattaa agccataaag aaacgcaaac
     2941 ctgaggtttt tataggtaaa agtgagattt ttatggtgca cattaagcgc cttttcccaa
     3001 gcatcttttt cagaattgta acaaaaatca aacctacata aacactatgt cgaaaattat
     3061 atacggtatc caacaggttg ggataggggt atcaaacctc tccgaggcat ggaaatggta
     3121 caaggagcat ctgggttttg atattcgaat ttttgaggat aatactgtgg ctgagcttat
     3181 gctcccctat acgggtggac aacctcaaca gcgccacgct gcacttgctc taaacatgca
     3241 aggaggtggt ggattggaaa tatggcagta taagggccga actccactta tgccagaatt
     3301 tcaggtattg cttggcgata aagggatctt tgcagcaaaa atcaaatgtc ccaatgtaaa
     3361 aaaagcctat gagtactttg aaagaatggg ggttcctgtt agccaacttg cggtaaatcc
     3421 aaatggcgat gagcattttt ttattaaaga tccatttggt aatttctttg aaatatacag
     3481 ccacagctac attttcaagg acgagaaaaa gcctgtttct ggggttggtg gagcaattat
     3541 tggcgttagc gatattgaaa acgcccttaa ggtttaccgt gatatcctgg gatacgatac
     3601 cgttttatac gacaaatcag gaaattttga tgatgtggca gaactccccg gaggcaaggg
     3661 aagcttccgt agaatgctac tcacccactc cgaaaaccga aaaggcagct tcagcaattt
     3721 ctttggtaaa tcggaaatag aactggttca ggcactcgat cgcgaaccca acaaaatatt
     3781 caaaaaccgt ttctggggcg atttagggtt tattcacctg tgttttgaca tgaaaaacat
     3841 gaacgacctg gaaaatgaat gcgtagctaa gagtttccca ttcactgtaa actccaacgt
     3901 aaagcacaac gaaaagggta gctttgacat gggtgaagca gccggtcatt tctcatacat
     3961 tgaggatccc gatgggactc tcattgaatt tgttgaaact caccgtctgc ccctcattaa
     4021 gcccttgggc atacagctta atatggataa acgcgaccca cataaaccaa ttcctaattg
     4081 gattacaaag ctgttaagcc taaaaagagt aaagaatttg tagaaatata aggctaccct
     4141 caaggtagcc ttaactttta ttccttttgt ttttaggaaa caacactctt ttgccagcat
     4201 gaataattgc aaaattctta aaccgagatt agtgaataga aacacaaaaa aaaacatgta
     4261 aatagcttgt ttctattggc ttatagaggc aatcccgact ctaaaatatt actgcgtcca
     4321 ttatttatta gcccggttga atttgttaat tcataatact ttggcttaac ggttcacgtt
     4381 taactattcc tctaactttc tctatctcta ccatattcaa aattcttctt ttaactctta
     4441 acggttcacg gttcacgttt aacgaactaa aaattcttct aacttcctct aactccatca
     4501 tattatgcct attccgcctc attcccgcct aaaatcacac atttaacttt tcacttttca
     4561 cttttcacaa ttcctctaac ttcctctaac tccatcatat tctgtctata ccgattcatt
     4621 cctcctcatt ccattaacaa tgtttctgtc tccccatttt ttagctacat catttctttc
     4681 atagcaacta ttggcttaat catttttgta tttttgaggg ttaagttttt gttaaatgcg
     4741 cataaattct attctttcaa ttttcctttt ggtacaaagt gcctattctc aaaccattaa
     4801 tcactgggaa acagccattt ttgccagcga tacctggcgg tatagggtaa accaaaccac
     4861 aaccccaaca ggatggacac tacacagctt tgaagatagt caatggcctc aagggcccgg
     4921 tggctttggg tacggcgata acgacgatgc cacagttgtt cccagctgca tatcggtaag
     4981 catcagaact acctttattg tttccgatgt tgatgaaatg gcaatggccg tgctaagtgt
     5041 ggattacgac gatgccttta ttgcatggat taatggagtt gaagttgcac gctctgcagg
     5101 tttacaggga gtaaatgtaa actgggatac accatcatcg gaaaatagag aggctatgat
     5161 gtacaccggt ggaaatccca ccgatttttt aataaaaatg gatgttctga gcagcgtact
     5221 aaggagtggt ttgaatgttt tggccataga ggttcacaac acgggtatca attcatcgga
     5281 tttgagtatc attccttttt tatcgtttgg tataacggtg aataacactt actatcgccc
     5341 tacaccggag tggtttaacc cacccgtaac ctcgcttagc agcaatttgc ccattgtgat
     5401 tattgatacc caaggccaag ccattcaatc cgacaacgaa atagtggcaa ccatgaaagt
     5461 tgttgataag caaagccccc ctaacagtat agccgataac gattttacct acaatggcac
     5521 tattcatctt gagtaccgtg gccaatcgtc gctatggaac gactggccaa agaaaagcta
     5581 caatgtcgag ctaattaacg cactgggcga aaacattgac tctgccctga tgggaatgcc
     5641 ccccggcaac gactgggcgc tttacgggcc ttacaacgat aaatcgctca ttcgtaacgc
     5701 tcttgcatac gaaattggta ggcgcatggg gcgctatgcc cccagaacgg ctttctgtga
     5761 gctaatcctc aacgaacagt atgccggcct ataccttctt attgagaaag ttcgtcgcga
     5821 taaaaaccgc gtgaatattg ctaaacttac ccaaaacgat atttcaggcg atgatttaac
     5881 tggtggctac atagttaaaa ttgataaggg tgattttgat gagttcggtt gggcatcgcc
     5941 ctatacacac aacggaaagc ccattaattt cctttggcac taccctaaac cagagaatat
     6001 attaccggtt caaaagaatt atatccgcaa ctacatcacc acctttgaga ataccctaaa
     6061 ctcatcgtac tggaacgacc ctttcctggg ctactaccgc tacgttaata tggcatcggc
     6121 tgttgattac tttttggtta atgaattatc aaaaaacatt gatgcctacc gcataagcac
     6181 tttcctgcac aaggataagg atagtaaggg aggtaagtta acctttggcc caatgtggga
     6241 ttacgacctt acctttggca atgccaacta ttacagtggc gactcgccac agggttgggt
     6301 tttcaaatcg attagcaccg acgattacta ccaacctccc ttttggtggg ataagtttac
     6361 caccgacgat aagttcaaca atatggctcg atgccgatgg gaagaactta aaagtagtgg
     6421 attactaacc cgtaccggtg tccatgccat tatcgatagc atgacaacgc taatacaaga
     6481 tgccagaata cgaaactaca ataccttcaa ctcacttggt gtatgggttt ggcctaatgc
     6541 atttgttgga agcacatacg agcaggaggt caactacctg aaaaatttcg tctccagccg
     6601 gttactttgg cttgatgcta acctacccgg ggtatgcaag gacgagccta atccaattat
     6661 gatctcagca atacccaatc cgttcaacga atacttcgac ctaagggtaa tactacccga
     6721 agtaggtcaa atcaccctaa aacttacatc catttcagga tcagtggttt ggcaaatgag
     6781 cgaaaatgca accctaacgt cgtttgtgta ccgatatcag gcccaggatt tgcagaaagg
     6841 gatttacata ctcacagcat atctcaatgg taagtatctt ggcgcggtta aggtgattaa
     6901 gggctaataa ggtttttact cttcagctcc aagcaaacgg ttaaccgtat caatcacttc
     6961 gtccagggca aagccccgcg aagcgccaaa ccgaattagc ttattacgca agtcgaacct
     7021 gtttttagcc tttacctggg gcattttctt gttaaggagg tcaaaaagat taaccggatt
     7081 ttcggcatat ctaagctcat taagggcctg atcgattata tcctttgcaa taccctttga
     7141 aacaagcatt gctctaagct ttattggtcc ccatttgttt attcggcttt tttccctaac
     7201 gtaaagccgg gcaaagcggt tatcgtccac atacccatcg gatgagagct tgccaactat
     7261 tttatcaatt tctgcttgag caaaacccca ctgcttaagc ttttgcacca aatcgtgagt
     7321 gcatttctcc tgtcgggcac aaaggtattg taagcgctcc atggcctttt ggggagttaa
     7381 cgctttatgg ttttcgcctt taatcatggg ttacactttt gggccttaac aaaccggggt
     7441 ttgcctctaa aatcgtcaat cagcaaaaca ttaccaaatc ctaatgattt taaaaggctg
     7501 acaatctctg aaccaagagc ctcattaatt tcaaaccaaa gccagcccat agtttccaaa
     7561 cgcctaacgg agtgtagggc tattgctcgg tagaacttta aggggtcgga atcggggaca
     7621 aacagagcaa tgtgcggctc aaagtccagt acattcctgt tcattagcac cttttccgaa
     7681 tccctaatgt agggcggatt gctaacaatg atatcgaagg tataaccgtc gagcactgtg
     7741 ggttgtaaaa tatcaccctg aaaaaaattc acattcaaat tatttgattt tgcattctgc
     7801 cgcgccacat caagggcatc ggtggaaata tcgattgcag caacgcttgc atcgggaata
     7861 tgctttgcta gtgcaagggc aatacaaccg cttcctgttc ctacatcaag aattcttggg
     7921 gcaacaatcc tattctcctt cacaattaac gacaccagct cttcggtttc ggggcgcgga
     7981 ataagaaccg agggcgtaac ctttaaaact aagtcggaaa agtaagccaa accaatggcg
     8041 tattgcaatg gcttaccgct tatgagttca gaaatgattc gttcaacctc ctgagcatcc
     8101 tcgtgcttaa cctcatgatg cttgttgata aacacctgat ggtatggaac atgtaacacc
     8161 gcctctgcta tgtacttttt tatggtgtgt agttcgttct cattgaaact tccactaaga
     8221 ctcctggaaa catgattaag taaaccgcta agggtgatat gctccataaa attgcttaac
     8281 tttgtgtaaa acaaaattac attttttatg gagaatgccg atgttcaccg aaaatacatg
     8341 gcccgatgcc ttgaacttgc cttgggtggg cagggtaatg ttgccccaaa tcctatggtt
     8401 ggtgccgtta ttgttcataa taaccaaata attggagagg gttttcacgc catgtacggg
     8461 caagcccatg ctgaggtgaa tgcaattaat tcggttcaga acaaggagtt actcaaagaa
     8521 tcaacactat acgtttcgct tgagccatgc tcacactatg gcaaaacccc accctgcacc
     8581 gacaggattg ttgaatacga aattccccgc gtggtgattg ccaccactga ccctaacccc
     8641 aaggtagccg gaagggggat tgagatactc agacaaaacg gcaaggaagt tattgtaggt
     8701 gtactggaga aggaggccat tgagcttaat aggcgtttta ttaccttcca taccaagaac
     8761 cgaccctatg taattttgaa atgggctcaa accatggatg gtttcattga taaaaagagg
     8821 gagcccaacg aacctgtagg ctcaaactgg ataaccaatg agctggcacg ttcacttgtt
     8881 caccgctggc gcagcgaaga acaggctgtg cttgttggaa ctaacacggt ggaaaaggat
     8941 aatccccagc ttaacgtacg gaactggagt ggtcgttcac caatacgcgt ggttgttgac
     9001 cgcaagctgc gcttgcccct taactccaat gtgtttgatg gaagccagcc aacccttctt
     9061 gttaccggaa acaatagtag cgctttggcg cgaaaaactg aattttcagg aatcgataac
     9121 ctggagatta taaccattga ctttgtaaaa ggcgtagagg ctcaaatact taaggaatta
     9181 gcagaaaggg aaatactttc agtaataatt gagggcggag caatgctact caacagcttt
     9241 attcagaaaa acctctggga cgaagcccga atgtttgtgg gcaataagt
//
LOCUS       DAOOWJ010000100         9273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_7384_length_9273_cov_6.795292, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000100 DAOOWJ010000000
VERSION     DAOOWJ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7384_length_9273_cov_6.795292"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLW22_09380"
     CDS             <1..666
                     /locus_tag="PLW22_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU74952.1"
                     /translation="VKSNPGMKLSKLVEDAGWNVKVYADGKGNSFKTPINSEPKSLIS
                     DKFEVKPKYSSSIERPVSIMLSGSAGEGVQSAAEFLAKAAIVSGLNSTKKGSYPVTVG
                     VGFSASEVIVSPKQILYTGSPVPDVLIITSTDGLGYARAAAGRMKGGVIYIDETLDAP
                     ETGARIVKVPFRERVGAKNSAMYAVFYAAHYEKFYPIEALKDVFLSNKIAEKVNIESL
                     LQF"
     gene            850..2052
                     /locus_tag="PLW22_09385"
     CDS             850..2052
                     /locus_tag="PLW22_09385"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU74953.1"
                     /translation="MEQQKKSPIIAILTGGGDVPGLNPAIRAITIRALREGFSVIGIR
                     RGWAGLIEIIRDKHVDNSHCFEVLTEERVNKAFRTGGTFLHTSRTNPAKVAKDNVPEH
                     LKAKYNKDINDLTPEVIKNLSFLGIDYLIPIGGDDTLSYGVRLYQEGFKVIAIPKTMD
                     NDVTGTDYCIGFSTCVTRTIALTDMLRTSAGSHERILVMEVFGRYAGFTAMLPTMAGV
                     ANRCVIPEYKFNIERLTELLVEDRNRNPSKYSTVLVSEGATFEGLEQMVFQSSERDAF
                     GHAKLGGIGEIVSRKIKELTPKFNNGKTINIISQNLGYLVRGGEPDAIDSIVPMAFGN
                     LALDLILKGVHGRLVVLKNGRYDNMPIDVVTSKKKIVNVERYYNTERLRPFYKSFEMQ
                     PLFIMTSE"
     gene            2056..2436
                     /locus_tag="PLW22_09390"
     CDS             2056..2436
                     /locus_tag="PLW22_09390"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOU74954.1"
                     /translation="MQIILQILDYFFVGLHTFIVLFNLFGWIWARFRRWNLALLILTG
                     LSWFGLGIFYGWGYCPLTDWHFRVLQGLGRTPQETSYISYLLNRLLQLKVDDTLVDSA
                     TLICFMVALVTSVILNVRGLRLKD"
     gene            complement(2549..3235)
                     /locus_tag="PLW22_09395"
     CDS             complement(2549..3235)
                     /locus_tag="PLW22_09395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HOU74955.1"
                     /translation="MKIVFILVEPAVPENVGAAARAFKTMGFNDLRLVNPCDYLSVDA
                     RKLAHASNDILENASVYNTLEEALHDIDFAFATSARDRWIKLDIIDSHEMPKFIEGKG
                     DTISNVAVVFGREESGLTNHEINLCQRVTTIPLKTKYPSLNLAQSVMVYAYILSGINL
                     GENQAAKADTTQSFQALSKKVDAVMKAIDLGPDKLIYGRLMERLSELGEQDIKLLHSI
                     TTELIQKLNR"
     gene            3348..4379
                     /locus_tag="PLW22_09400"
     CDS             3348..4379
                     /locus_tag="PLW22_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU74956.1"
                     /translation="MKKILLFAIFASGVTITAAQVKLTQETHGFFADLKNPMVLTSYV
                     EPGQSGSNVVWDFSKLQVQNSFTGNIEGFYTSKCCSKFTKGNVVLEEFGNFFVFESTP
                     TSLEQVGYMSANGVTRFEYSKPFIKMRYPFSYGDFYSGNYEGNYIVNDNVIGTISGTY
                     QVEGDGFGKLILPNGQTLNNVLRVKEVRTTKQVFSSGSALITDITYRWYVAQHRFPVL
                     VLIRSEVKGENSEPVLSTRAAYNSNVMNATTSIPTNQISALSLSAYPNPYTDRVKIEF
                     TMAQQDQVNLSIYDMSGKLVKEIENNTLDSGLKHYEFSAKSLGLTSGAYILKLKVGQI
                     EETRKLLEL"
     gene            4898..6259
                     /locus_tag="PLW22_09405"
     CDS             4898..6259
                     /locus_tag="PLW22_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOU74957.1"
                     /translation="MFGRDSFNKHRVRELKVGEVKGKGPVIYWMSRDQRVADNWALIY
                     AKLKANEHKVPLRVVFTLTAAFPAANLRHYDFMLKGLAEVEKSLAHEGIPFNLLRGEP
                     AEQLWEFAERVEASLIVSDFDPLWVKRDWKSQLNQRITIPHHEVDAHNVVPCWYASPK
                     LEFGAYTLRPKIKKHLSTFLTDFPDVKLQCKSEPNPHLTRWHEVLVWLNVSDEVKPVK
                     AFEAGENAAHAMLSEFISLRLKGYAEKRNDPTLEWQSNLSPYLHFGQISAQRVAIEVL
                     KAPAPEADKQALLEELIVRRELSDNFCFYNPGYSRFAGFPDWAKESHRIHRFDVREYL
                     YTLNEFEIAHTHDPLWNAAQLQLVNKGKMHGYMRMYWAKKILEWTTCPEEAMRFAIYL
                     NDKYSLDGRDPNGYTGIAWSIGGVHDRAWPERPIFGKIRYMNFDGCKRKFDVDKYIKM
                     NLD"
     gene            6301..7524
                     /locus_tag="PLW22_09410"
     CDS             6301..7524
                     /locus_tag="PLW22_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HOU74958.1"
                     /translation="MKRFIHLVSIFTIGALLFQSNLSKAQANKSPDGYVLLISLDGFR
                     WDYPNMYNTPNIDAFGRSGVRAQSLISCYPSKTFPNHYSIATGLHPDHHGIVNNSFYD
                     SDLGYYRLGDRMSVENGKFYGGEPIWVTAEKQGIKTASFYWVGSEAPIQGIQPTYWKR
                     YDQKISFVQRVDTVIKWFTLPIEKRPQLVTFYYHEPDWVSHDFGPVSPQTQKVVEQLD
                     SLIGYFLNRLAELPIAGNLNIIILSDHGMTAISSARVVNLSEYFSKDIFKYISGGNPV
                     YTLQPKPEHYREVLAKLKSIPHLKVWERNKIPKRYIYGNNPRINDILVEAELGWSVTW
                     SHDKESYTGGTHGYDNLIPDMQGIFYARGPAFKSGYVHPSFFNVNVYSIIANILNLKP
                     AKTDGNLEEVQGMLK"
     gene            complement(7557..8603)
                     /locus_tag="PLW22_09415"
     CDS             complement(7557..8603)
                     /locus_tag="PLW22_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU74959.1"
                     /translation="MVILPVAAQTEQNLENKVNELLKENESLNHRLDVLEKKIDDVTW
                     FHRVGDLAFIDKVYIYGPPPANVKNPEQTGATNPVKFWSYVFIPKNIDPNKKYPMLVF
                     PHGGVHGDFTTYYTHIVREMLAQGYIIVAPEYRGSTGYGSEHWHRIDYGGLENEDVFA
                     SRNYMIENYDFIDKNRVGIIGWSHGGMIALHNIFDHPDSYACAFAGVPVSDMILRYGY
                     AADDYRKLYDADYHIGKTPREDINEYRRRSPAWQAHKLKTPLLIHTNTNDDDVYVIEV
                     EALIKSLKAEGKKFEYEIFQDMPGGHSFDRIDTKAARQVRVKIYKFLAQYLNPPKPIK
                     SVEDINRAAYLPMK"
     gene            complement(8883..>9273)
                     /locus_tag="PLW22_09420"
     CDS             complement(8883..>9273)
                     /locus_tag="PLW22_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU74960.1"
                     /translation="GYILVLFIQMLGYFSIALLIIVLTRNIALTIIVYLLYFALIEPI
                     IRLFLPDSAAQFLPVKIISNLTPMPDFLGIMAKQFNVNDQLDASTLNALDAINNGTSL
                     TQNIVIAIIYIALFTVLSYYVFRRKDF"
BASE COUNT         2588 a         1910 c         2074 g         2701 t
ORIGIN      
        1 gtaaaatcga atccgggaat gaagcttagc aagctcgttg aggatgccgg ttggaacgtt
       61 aaagtttatg ccgatggcaa gggtaattcg ttcaaaacac ctattaacag tgaacctaag
      121 agtttgattt cggacaaatt tgaggtgaaa cctaagtact ccagcagtat agagaggcca
      181 gttagcataa tgttgtcggg ttctgctggc gagggtgttc agtcggctgc cgaattcctg
      241 gcaaaggctg ccattgtatc gggtcttaat agtaccaaaa aaggaagtta cccggttacg
      301 gttggtgttg gattctcggc ttcggaggta attgtttctc ctaagcagat cctttacact
      361 ggctcgccgg ttcccgatgt acttataatc acatcgaccg acggtttggg ctatgcccgc
      421 gcagcggctg gcagaatgaa aggaggagtt atctatattg atgaaacatt ggatgcgcct
      481 gaaaccggtg ctcgcattgt taaagttcct ttccgcgaga gggtaggggc aaagaatagc
      541 gccatgtatg ctgttttcta cgccgctcat tatgaaaaat tctacccaat tgaggctctc
      601 aaagatgttt tcctttccaa taaaatagcc gaaaaagtaa atatcgaaag tttgttgcag
      661 ttttgaccta gatatcgata ggaacacaat aaatagttaa actatttgct tctatcgacg
      721 aacttgggtt taagttagtt tatttgaagc atttttaggg gctgcccaat atgggtagct
      781 ctttttttct attgacttta gctcagaata aacttatatt taatcgtttt taaattaaaa
      841 tttaaagcta tggaacagca aaagaaatct ccaattattg caatacttac cggcgggggc
      901 gatgtgcccg gacttaaccc agccatacga gctataacca tcagggcgtt acgcgaaggt
      961 tttagcgtga ttggaatacg gcgtggttgg gctggtttga ttgagattat ccgcgataag
     1021 catgtggata atagccattg ctttgaggtt ttaactgagg agcgtgttaa taaagcattt
     1081 cgtaccggtg gaacattttt acacacctcg cgcacaaacc ctgctaaggt tgcaaaggat
     1141 aatgtacctg aacacctaaa agcaaaatat aacaaggata taaacgattt aacacccgag
     1201 gttattaaaa atctatcttt tttgggcatc gactacctaa tacccattgg gggtgacgat
     1261 accctcagct atggcgttag gctttaccag gaagggttta aggtgatagc tatccctaaa
     1321 accatggata acgatgttac cggaaccgat tactgcatag ggtttagcac ttgcgttaca
     1381 cgcactattg cattaaccga tatgttacga acctcggcag gctcgcatga acgaatactg
     1441 gtgatggagg tttttggccg atatgccgga tttaccgcca tgcttcctac tatggctggc
     1501 gtggctaacc gctgcgtaat acctgagtac aaattcaaca ttgaacggct taccgagctg
     1561 ttagttgagg atagaaatcg aaacccaagt aagtactcaa cagttttagt ttcggagggg
     1621 gctacctttg aagggcttga gcaaatggtt ttccagagta gtgaacgcga tgcatttgga
     1681 catgctaaac tcggcggtat tggtgagatt gtttcccgaa agattaagga gctaacgccc
     1741 aagttcaaca acggtaaaac tataaacatt ataagtcaga accttggcta tttggttcgt
     1801 ggcggggagc ccgatgctat tgattccatt gtccccatgg catttggcaa ccttgcactc
     1861 gatcttatcc tgaagggtgt tcacggaagg cttgtagttc ttaagaatgg tcgttacgat
     1921 aatatgccta ttgatgtggt taccagcaag aagaagattg tgaacgtgga gcgttactat
     1981 aatactgaac gtttacgtcc gttctataag agctttgaga tgcaaccact gttcatcatg
     2041 accagcgagt aaaaaatgca aataatcctt caaatacttg actacttttt tgttgggttg
     2101 catacattca tagtactatt taatcttttt ggttggatat gggccaggtt ccggaggtgg
     2161 aatctggcct tgcttattct tacaggtctc agctggtttg ggcttggcat attctacggt
     2221 tggggctatt gtcctttaac tgattggcac ttcagggtac tccaaggact tggcagaaca
     2281 cctcaagaaa catcgtatat atcctatttg ttaaataggc ttttacagct aaaagttgac
     2341 gatacactgg ttgatagcgc taccctaatt tgttttatgg tggctttagt tacttcagtt
     2401 attctgaacg ttaggggatt aaggttaaag gattaaggtt aaaggataaa ggataaagga
     2461 taaaaaagtt gaaggatgaa tgggtttcat attgtattga tatgagatac tgacttctga
     2521 ttcattgctt tccatcctat ttttccattt acctgttcag tttctgaatt agctcggttg
     2581 ttatggagtg cagcagttta atatcctgct caccgagttc gctgaggcgc tccatgagcc
     2641 taccgtatat tagcttatcg ggtccaaggt caatagcctt catcactgca tcaacttttt
     2701 ttgaaagtgc ctgaaacgac tgggtggtgt cggctttggc tgcttggttc tcgcctaggt
     2761 tgattcccga tagaatatag gcataaacca tcaccgattg tgccaggttt agcgatgggt
     2821 atttagtttt aagcggaata gtagtaaccc tttggcaaag atttatctcg tggttggtaa
     2881 gccccgactc ctctcggcca aatacaacgg ccacattgct gattgtgtcg ccttttccct
     2941 caataaattt cggcatttca tggctgtcga taatatccaa ctttatccac ctatcgcggg
     3001 ccgacgtggc aaaggcaaag tcgatatcgt gcagtgcttc ctctagtgta ttataaacac
     3061 tggcattctc aaggatatcg ttagaggcgt gtgccagctt gcgggcatca accgaaaggt
     3121 aatcgcaggg gtttaccagt cgtaagtcgt tgaaacccat tgttttaaag gctcgggcgg
     3181 ctgcgccaac attctcgggt acagcaggtt ccacaagaat aaatacaatt ttcatctctt
     3241 ttaggaatta tcgcaaaatt aacaatgaaa ttagaactat ttgccttaat ctattgattt
     3301 gagttggctg tatgattaaa tttgttttta cacaaaaggg taatcgtatg aaaaagattt
     3361 tactatttgc catttttgca tcgggcgtaa ccattactgc tgcccaggta aagctaacgc
     3421 aggaaaccca tggtttcttt gccgatttaa agaacccaat ggttctaact agctatgttg
     3481 agcccggaca gtcggggagc aacgtggtat gggacttcag caagttgcag gttcagaatt
     3541 cttttaccgg taatattgaa ggtttttaca cttcaaagtg ttgcagcaag tttaccaagg
     3601 gtaatgtggt acttgaggag ttcggaaact tctttgtttt tgaatcaacc ccaacaagtc
     3661 ttgagcaggt tggttacatg tcggcaaatg gggtaacccg ctttgagtat agcaaaccct
     3721 ttattaaaat gcgttaccca tttagctatg gcgattttta cagtggcaat tacgagggta
     3781 attatattgt aaacgataat gtaattggta caatctccgg cacttaccag gttgagggcg
     3841 atggctttgg aaagttgata cttccaaacg gtcaaacact taataatgtg cttcgtgtta
     3901 aggaggttag aaccaccaag caggtattca gtagcggttc ggctttaatc accgatataa
     3961 cctaccgttg gtatgtagct cagcataggt ttccagtact agtcctaatc cgtagcgagg
     4021 tcaaaggcga gaattcagaa ccagttttaa gtacacgtgc tgcgtataat agcaatgtga
     4081 tgaatgctac cacatcaatt ccaaccaatc aaatatcagc tctcagctta agcgcttacc
     4141 ctaatccata caccgatagg gtcaaaattg agtttaccat ggcccaacag gatcaggtta
     4201 accttagcat ctacgatatg agcggcaagc tggtaaagga aattgagaac aacaccctcg
     4261 attctggcct aaaacactat gagttctcag caaagtccct gggcttaacc agtggtgctt
     4321 acatactcaa gctaaaggtt ggtcaaattg aggaaacacg caagctgttg gagttataga
     4381 tgttaaatat ctttattgaa ggccgggtaa tcccggcttt tatattaacc aagactagtc
     4441 aatagaaata cattaaaaca ttcaaataac ttgtttctat tgacttatcg aggtaatccc
     4501 gacttatcaa aatcttacat ttcgatatct cggaacgatt agattttgtt agacgattcg
     4561 tcaattgaaa aattcaattg gaaatatcct ctaataatgt ttgcgcatcc ccattttttt
     4621 aagtcctttt ttatttgata attcatggta cttcggttta gctgttcact tttcactttt
     4681 cacttttcac aattcctcta acttcctcta acttcctcta actccatcat attctgccta
     4741 ttccgattca ttccgactca ttcatcaaaa atcagcatgg ttgaatttgc taattcatgg
     4801 ttcatgggca tgatattgac ctatttccct aaaatttatt aaacagattt caagaactca
     4861 atgtttttgg ctatttttac cttaaacctg taaattgatg ttcggtaggg atagctttaa
     4921 taaacatagg gtaagggaac ttaaggtggg cgaggtaaaa gggaagggcc cggttatcta
     4981 ctggatgtcg cgtgaccaac gcgttgccga taactgggcg cttatttatg ccaagctaaa
     5041 ggcaaatgag cataaggttc ccctccgggt tgtatttacc cttaccgcgg cattccctgc
     5101 tgccaatctc cgtcattacg atttcatgct caaaggcctt gcggaggttg aaaaaagcct
     5161 tgcccatgag ggaatccctt tcaacctcct taggggcgaa cccgctgaac agctttggga
     5221 atttgccgaa agggttgaag cttcacttat tgtaagtgat tttgacccgc tttgggttaa
     5281 acgcgattgg aaatcgcagc ttaaccagcg tataaccata ccccatcatg aggttgatgc
     5341 tcacaatgtg gttccctgtt ggtatgcttc gcctaaattg gagtttgggg cgtataccct
     5401 tcgacccaag ataaagaaac acctatcaac cttcctgacc gattttccag atgtaaagtt
     5461 gcagtgtaaa tcggaaccta atccccatct tacacgttgg cacgaggtgc tggtttggct
     5521 caatgtttcc gatgaggtta agcctgttaa ggcttttgag gccggcgaga acgctgccca
     5581 cgcaatgctc tccgaattta tttcgctcag gctaaagggc tatgccgaaa aacgtaacga
     5641 ccccactctc gagtggcaat cgaacctatc gccctatttg cattttggcc aaatatcggc
     5701 ccagcgagtt gccattgagg tgctaaaggc acccgcgcct gaagccgata agcaggcatt
     5761 actggaggag ttgattgtta ggcgtgaact ctccgataat ttctgttttt acaacccggg
     5821 ttattcccga tttgccggat ttcccgattg ggctaaggaa tcgcaccgca ttcaccgttt
     5881 cgatgttagg gaatacctct ataccctaaa cgaattcgaa attgctcaca cgcacgatcc
     5941 actttggaat gctgcacagc tgcagctggt aaataaaggg aaaatgcatg gctacatgcg
     6001 tatgtactgg gctaaaaaga tactggagtg gactacctgc cctgaggaag ccatgcgttt
     6061 tgccatttac ctgaacgata agtacagcct cgatggtcgc gatcccaatg gctatactgg
     6121 aattgcatgg tcaattggag gggtgcacga cagggcttgg cctgaaaggc caatatttgg
     6181 taaaatccgt tacatgaact ttgacggctg taaacgtaag ttcgatgttg ataagtatat
     6241 taaaatgaat ctagattaaa cctttagccc tttggtacgt ttaactgtaa acaaattccg
     6301 atgaaacgat ttatacattt agtatcgatt tttaccattg gagccttgct atttcaaagc
     6361 aatctatcta aagctcaggc taacaaatca cccgatggct atgtcttact catttcgctc
     6421 gatggcttta ggtgggacta cccaaatatg tataacacac caaatatcga tgcatttgga
     6481 cgaagcggcg ttcgtgcaca atcgctcatc agctgctatc catcaaaaac ctttcccaac
     6541 cattacagca ttgcaaccgg tttgcacccc gaccatcacg gtattgtcaa caattcattc
     6601 tatgattcgg atttaggcta ctatcggctt ggcgacagga tgagcgttga aaacggcaag
     6661 ttttacggtg gcgaaccaat ttgggtaacc gccgaaaagc aaggcattaa aacagcatcg
     6721 ttttactggg taggttccga agccccaata caaggtattc agcccaccta ctggaaaagg
     6781 tacgaccaga aaatttcgtt tgtgcagcgt gtcgacaccg taattaagtg gtttactctt
     6841 cctattgaaa aacgccccca attggttacc ttttactacc atgaacccga ttgggtcagt
     6901 cacgactttg gccctgtttc gcctcaaacc caaaaggttg ttgagcagct cgatagttta
     6961 atcggatatt tccttaatcg cttggcggaa ttgccaattg ccggaaatct gaatatcatc
     7021 atcctgtccg atcatggcat gacagcgatt tcgtccgcta gggtagttaa cctttcggag
     7081 tatttcagca aggatatctt caagtacata tcgggaggga accctgttta cacactacaa
     7141 cccaaacccg aacattacag ggaggtgcta gccaaattaa aatccattcc tcacctcaag
     7201 gtgtgggagc gcaacaaaat tcctaaacga tatatttacg gcaacaaccc tcgtataaac
     7261 gatatactgg ttgaagccga gttaggctgg agtgtaacat ggtcgcatga caaggaaagt
     7321 tacaccggtg gaactcacgg ctacgataac ctaatccccg atatgcaagg aattttctat
     7381 gcccgtggcc ccgctttcaa aagcgggtat gtgcacccgt cgtttttcaa cgttaacgtt
     7441 tactctatta ttgccaacat cctgaaccta aaacccgcaa aaaccgatgg gaatttggag
     7501 gaggttcagg gaatgctgaa ataaaaaaaa gaagggtgtt tacaccctac ttttttttat
     7561 ttcataggca agtacgccgc cctgttgata tcttcaaccg attttattgg tttgggcggg
     7621 ttaaggtact gagccaggaa cttgtaaatt tttactctaa cctgccgtgc agccttagta
     7681 tcaattctgt cgaatgaatg gccaccgggc atatcctgga atatctcgta ctcaaacttt
     7741 ttcccttcgg ccttaagtga ttttataagg gcttccactt caataacata cacatcgtcg
     7801 tcgttggtgt tggtgtggat tagcagaggg gtttttagct tatgggcttg ccatgccggc
     7861 gatcgacggc ggtactcgtt aatatcttct cggggagttt tgccaatatg gtagtcggca
     7921 tcgtaaagct tgcggtagtc atcggcggcg taaccatagc gcagtatcat gtccgatacg
     7981 ggtacgccgg caaaagcaca ggcatagctg tcggggtggt caaaaatgtt gtgcagggca
     8041 atcatgcctc cgtggctcca tcctataatg cccacacggt ttttgtcaat aaaatcgtag
     8101 ttttcaatca tgtagtttcg gctggcaaaa acatcctcgt tttccagccc accataatca
     8161 atccgatgcc aatgctctga accgtaaccc gtactacctc gatattcagg tgctacaata
     8221 atatatccct gagcaagcat ttctctaaca atatgtgtat agtaggtagt aaaatcgccg
     8281 tgaaccccac catgcggaaa aacgagcatg ggatatttct tgttggggtc tatatttttt
     8341 ggaataaata catagctcca aaacttaaca ggattggtgg cacctgtttg ttcgggattt
     8401 ttaacatttg caggaggagg gccataaatg taaaccttgt cgataaaagc taagtctcca
     8461 acccgatgga accaggtaac atcatcaatt tttttttcca gcacatcaag cctgtggttt
     8521 agactctcat tctcttttag taactcatta accttgttct caaggttttg ttcggtttgt
     8581 gcagccactg gcagtattac catggccata aggaatgcag atagatatcg tttcataaag
     8641 tattttttta tattgattct tttaacttca aatggtttag caaagataaa taattaagcc
     8701 gaataggcaa ggagaatgga taggtgggaa acgtgaatag ttataagagt tagctaaacg
     8761 ttaaccttta aacgggaatg attcggcatg attcggaatg aaggagttac aggaagttaa
     8821 aggagaattg aaaagttaaa tgaggctctt gaatccccct ttgcgtggca gtttttttat
     8881 ccttaaaagt ctttccttct aaaaacatag tacgataaaa ctgtgaaaag agcaatgtaa
     8941 atgatagcaa tcacaatatt ctgggtaagc gatgtgccat tatttatggc atcgagggcg
     9001 tttaaggtgc tggcgtctaa ctggtcgttt acgttgaact gctttgccat aatgcccagg
     9061 aaatcgggca tgggagtgag atttgaaatt attttaacag gcaaaaactg agcagcagaa
     9121 tcaggtagga ataggcgaat aattggttca attagagcaa agtatagcag gtaaactata
     9181 atggttaagg caatattcct ggtaagtaca ataattagga gcgctatgga aaagtagcca
     9241 agcatttgaa tgaaaagtac cagaatgtaa ccg
//
LOCUS       DAOOWJ010000101         9148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_7534_length_9148_cov_7.632904, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000101 DAOOWJ010000000
VERSION     DAOOWJ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7534_length_9148_cov_7.632904"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLW22_09425"
     CDS             <1..672
                     /locus_tag="PLW22_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU74961.1"
                     /translation="SFIAFICSNLLAITLLSMTLNYTIFFYNRKFIITSDPQEVLIAD
                     STFFCWGDTKPEDISKLIDFFQSHTEVSTLFACTSDPKTTMKHFTNSFNHIEAAGGVV
                     CNDKNEILLIKRLGRWDLPKGKVEPNEPIHDAALREVSEETGVKGITLGKQITTTYHT
                     YTLNGKQMLKSTHWFNMYTPKCDTLKPQTEEGIEQVKWVKRSDIFVYLADSYSSLLSV
                     LKELD"
     gene            complement(669..2084)
                     /locus_tag="PLW22_09430"
     CDS             complement(669..2084)
                     /locus_tag="PLW22_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU74962.1"
                     /translation="MAKFKFLLLFLLLFIGVECKIGAQEAMIFGNAAEYKGSDIIFYT
                     YSDYITETEYEVGRCKVERDGKFNAKLKVLETSFVFAHLGVYRIYFFAEPGKRYELVL
                     PPREDKTEAQRLNPFFRETDLQVGIKNVDRNDINFLINAFDLRFNQDFDQIVQDAYRG
                     RQHNIDSLITRIEEKFAGTGNQFFDAYREYRYGLLRQITFIKKSTATSKELFLNRPIL
                     YRNPAYMELFNLVYDKYFLFFSRTSQGNAIFDDISRYKSLGRLKRTLATDDVLSNDTL
                     MEMVILKSLHDEFFSDNFSRSALLTILDSIYRTTTIPEHVIVAENIRNRITKLLPGFV
                     PAPFSLNDVNGREVSLDRFKGKYVYLNFCTTTSYTCLKEFVQLEKISKQYKKHLEVVT
                     ISADDNIDDLKSFLENSKYDWTFLHFGNKPEILKDYDIRVYPTYFLIGPDRKMILSPA
                     PSPEEGFERRFFQLLRSKGEI"
     gene            complement(2089..2559)
                     /gene="coaD"
                     /locus_tag="PLW22_09435"
     CDS             complement(2089..2559)
                     /gene="coaD"
                     /locus_tag="PLW22_09435"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOU74963.1"
                     /translation="MEKVALFPGSFDPITVGHESIVYRSLSLFDKVIVAIGYNANKKA
                     FFSIEKRIAMIQKVFAQEPRVQVISYEGLTVDLCNRLGVRYILRGLRTSADFEFERAI
                     AQVNKLMHPNIETVFMLTAPQHTPVNSTIIRDILLHNGDPSQFIPSAINIKDYL"
     gene            complement(2679..5135)
                     /gene="lon"
                     /locus_tag="PLW22_09440"
     CDS             complement(2679..5135)
                     /gene="lon"
                     /locus_tag="PLW22_09440"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOU74964.1"
                     /translation="MKKKFGLSILNQQMDDTNDSEVNFIPILAEGEVPEVKKADIPDV
                     LPIMALRNTVLFPGVLMPITVGREKSLNLIASLPPKKKIIGVLSQVDARIEDPSSGDL
                     FKVGTIAQVIKVLELPDGNSSVILQGLSRFRVDQFVSEEPYFMARVLELAELLPNEDD
                     TEFIALTESIRDLTIRIIHLSPNIPKEASFAIKNIDNPKMLVNFISANSDIKTDEKQA
                     LLEEHDLKRRAEQLLRILTRELQMLELKHDIQNKVKHDLDQQQREYFLQQQMKTIQDE
                     LGGNPIEQEINELKERAKAKKWSKEVAEHFDKEVNKLNRMNPMAGEYSVQLNYVQLLL
                     DLPWNEYTNDNFNLDHAKNILDEDHFGLEQVKERILEHLAVLKLKGDLKAPILCLYGP
                     PGVGKTSLGKSVARALGRKYARISLGGMHDEAEIRGHRKTYIGAMPGRIIQSLKKVKS
                     SNPVIILDEIDKVGKDFHGDPASALLEVLDPEQNSTFHDNFVEVEYDLSKVLFITTAN
                     TVATINPALRDRMEMIEVSGYSLEEKVEIAKRHLLPNQLKEHGLNSDALELSDKVLAK
                     VIQEYTRESGVRNLNQKIAKLVRYAARMIASEQKYNPKLTNKDIEKILGVPRYTKELY
                     QGNDIAGVVTGLAWTEVGGEILFVETSLSRGKGNLTLTGNLGEVMKESAILALEYIKS
                     HADYLGIKHEVFEKWNVHIHVPEGAIPKDGPSAGITMVTSLASAFTQRKVRANVAMTG
                     EITLRGKVLPVGGIKEKILAAKRANITDIILSADNRKDVSEIDATYVKGLKFHYVSNI
                     HEVLDLALLNEKVDKPMNID"
     gene            complement(5349..6539)
                     /locus_tag="PLW22_09445"
     CDS             complement(5349..6539)
                     /locus_tag="PLW22_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOU74965.1"
                     /translation="MINTIVLFDDQRRDDLLPLTFTRPVAEIRMGILTISQKWSMLTG
                     ASVHYLTQDYLQTKYPNAPKGEPVLLVNGGICPDSNLVDKIRALKPNQAIVKGDTLIA
                     AMLNSTVDTLPAAGLFEKTFEYPDDILEVKYPWDIFRNNGEAIVRDYSLITKGRKSQP
                     ISATNTVLNPDNVFIEEGARVECAILNAETGPIYIGKDAEVMEGSIVRGPFALGEHSA
                     LKMGAKIYGPTTVGPYCKVGGEVNNSVFFAFSNKAHDGFIGNSVIGEWCNLGADTNNS
                     NLKNNYASVKLWSYRKKGFVDTGLQFCGLIMGDHSKCGINTMFNTGTVVGVSANIFGD
                     GFPRNFIPCFSWGGAAGFTTYQPQKAFETARIMMSRRDLPFTDIDKEILLKVFELTRE
                     YRMY"
     gene            complement(6642..6902)
                     /locus_tag="PLW22_09450"
     CDS             complement(6642..6902)
                     /locus_tag="PLW22_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOU74966.1"
                     /translation="MKKGIHPENYRLVAFKDMSNEHVFITKSAVNTKDTITIDGVEYP
                     LVKVEISNTSHPFYTGKVKLVDTAGRVDKFMNRYKKHMDNKK"
     gene            complement(7014..7769)
                     /locus_tag="PLW22_09455"
     CDS             complement(7014..7769)
                     /locus_tag="PLW22_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOU74967.1"
                     /translation="MNLTKSSNPALSDRIFSKAGYASGAEVMTLQGTLNKALLMLALV
                     ILGAAYTWRIFFESVNPASVVPWMFVGGIGGFITSLVVIFAPKTSPYTAPVYALLEGI
                     FLGGISAYFASSYGAGIIMQAIGLTLAVFFLMLLAYRSGTIRATGKFKVMVLAATGAI
                     ALTYFISFVLSFFGISLGFIHGSGTFGIIFSLIVVGVAALNIIIDLDFIDRASASGAP
                     KFMEWYGAFGLMVTLIWLYIEILKLLAKLNRRN"
     gene            complement(7834..8493)
                     /locus_tag="PLW22_09460"
     CDS             complement(7834..8493)
                     /locus_tag="PLW22_09460"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOU74968.1"
                     /translation="MATLEKVLEIKSQIIHRASGEVAENMRMAGIIYKVNYGVPIPEL
                     KELAKPYKGDHDLALELYAEDIRECKILASLIADPERLTGEQIDEWAAEFTNPEIVEQ
                     VCGNLFWKSEFALSRSIEWCLSNDELLRKAGLLIIARKASDTAIKESLLEPYIGIIEN
                     MAEEMTDLTQSAVQFALREIAKRSPELAARVSETASRMTESENELAAWVGNELLFELT
                     E"
BASE COUNT         2609 a         2107 c         1848 g         2584 t
ORIGIN      
        1 agctttattg cgtttatttg cagtaattta ttggctataa ctttgctatc catgactttg
       61 aattatacca ttttctttta caatcgcaaa tttattatca cttcggaccc ccaagaagtg
      121 ctcatagccg actctacatt tttctgttgg ggcgacacta agcccgaaga catttcaaag
      181 ttaatcgatt ttttccaaag ccataccgag gtgtccacac tttttgcctg cacctccgac
      241 cccaaaacaa caatgaaaca tttcactaac agctttaacc acattgaggc agccggtgga
      301 gttgtatgca atgacaagaa tgagatttta ctcattaagc gtttgggccg ctgggatttg
      361 cccaaaggca aggtagagcc aaacgaacct attcacgatg ccgctttacg cgaagttagt
      421 gaggaaaccg gggtaaaagg cattactctt ggaaagcaaa ttacaacaac ctaccacacc
      481 tatactttaa atggcaagca aatgctaaaa tccacgcatt ggtttaatat gtatacccct
      541 aaatgcgata ccctaaaacc ccaaaccgaa gagggaatag aacaggtaaa gtgggttaag
      601 cgttccgata tctttgtata ccttgccgat agctactcaa gcctgctctc tgttctcaag
      661 gagttggact aaatttcccc tttgcttcgg agcaattgaa agaatcggcg ttcaaagccc
      721 tcctcgggcg aaggcgctgg tgaaagaatc attttccggt caggcccaat caaaaaatac
      781 gttggatata cacgtatatc gtaatccttg agtatttccg gtttattccc aaagtgtagg
      841 aatgtccaat cgtacttact gttttccaaa aagcttttta aatcatcaat attatcgtcg
      901 gctgaaatgg tgacaacctc cagatgcttc ttgtactgct tgcttatttt ttcaagctga
      961 acaaactcct ttaaacaagt gtagcttgtg gtggtgcaaa agttcaggta aacatacttg
     1021 cccttgaatc tatcaagcga aacctcacga ccattaacat cgttgagcga aaaaggtgca
     1081 ggaacaaaac ccggtaaaag cttggtaatc ctgttcctga tattctccgc aactatcaca
     1141 tgttccggta tggtggtggt tctgtaaatg gagtcaagaa tggtaagcag agccgatcgg
     1201 gagaaattat cgctgaaaaa ctcatcgtgc aacgatttaa gaattaccat ctccatcagg
     1261 gtatcgttac taagcacatc gtcggtggca agggtacgct taagcctgcc caaactcttg
     1321 tagcgcgaaa tatcatcaaa aatggcattc ccctgcgatg ttcttgaaaa gaacaaaaag
     1381 tacttgtcgt aaaccagatt gaacaattcc atgtatgccg ggttacggta caatataggc
     1441 ctattcagga acagctcttt tgatgtggca gtagattttt ttatgaaggt gatttgcctg
     1501 agcaaaccgt acctgtactc acggtaagca tcgaaaaact ggttgccggt tccggcaaac
     1561 ttttcctcaa ttctggttat taacgaatcg atattatgct gacggccacg gtatgcatcc
     1621 tgcacaatct gatcaaaatc ttggttgaag cgcaagtcga atgcatttat caggaagttg
     1681 atatcgttcc tatcaacatt ttttatgccc acctgcaaat cggtttcacg gaaaaaagga
     1741 ttgagtcgtt gtgcctcggt tttatcctcg cgagggggaa gcactagctc ataccgttta
     1801 ccaggctcgg caaagaaata aatcctataa actccaaggt gggcaaaaac aaagctggtt
     1861 tcaagcactt taagctttgc gttaaacttg ccatcgcgct caaccttaca cctaccaacc
     1921 tcgtattcgg tttcagttat gtaatcggaa taggtataaa atattatatc ggagcccttg
     1981 tattcagcag cattgccaaa aatcatggcc tcctgggcgc cgattttaca ctcaacgcca
     2041 ataaacagca gtaagaataa taaaagaaac ttaaatttag ccataagttt aaagataatc
     2101 cttgatgtta atggctgatg ggataaactg cgatgggtcg ccattatgca aaagaatatc
     2161 gcggataatg gttgagttaa cgggtgtgtg ctggggtgca gtaagcatga ataccgtttc
     2221 aatattaggg tgcattagct tatttacctg cgcaatggca cgttcaaact caaagtcggc
     2281 tgaagtacgt agtccccgta ggatgtaccg aacgccaagc ctattgcaaa gatctacggt
     2341 aaggccctcg tagctaatca cctgaacacg tggttcttga gcaaacacct tttggatcat
     2401 ggcaatcctt ttctcaatgc tgaaaaaagc ctttttattg gcgttatagc caatagcaac
     2461 tatcacctta tcgaaaaggg ataatgatcg gtaaacaatt gattcgtgac cgactgtaat
     2521 aggatcgaac gaacccggaa acagggcaac tttttccatg gctaagttta tttagctcca
     2581 gcaattttca tacaattata ggtaaaattg acgacttaat agttaataaa caaaaaaaga
     2641 cccttaattc agggtctttt catgcattat attcacggct aatcaatatt cataggttta
     2701 tcgaccttct cgtttagcaa agctaaatca agcacctcgt ggatgttgct aacgtaatgg
     2761 aatttcaacc ctttaacata tgtagcatca atctcggaaa catccttgcg attatcggcc
     2821 gaaagaatga tatcggtaat gttggcacgt ttagctgcaa gtattttctc cttgatacca
     2881 cctactggca gtaccttgcc tctaagtgtt atctccccag tcattgctac gtttgcccta
     2941 actttacgct gggtaaatgc cgatgctagt gaggtcacca tcgtaatacc agccgaaggc
     3001 ccatcctttg ggattgcccc ttcgggtaca tggatgtgta cattccattt ctcaaaaacc
     3061 tcgtgtttaa ttccaagata atcggcgtgc gattttatat attcaagggc cagaatggcc
     3121 gactctttca ttacctcgcc cagattaccc gtaagggtga gattaccttt gccgcggcta
     3181 agactggttt ccacaaaaag tatttcgcca ccaacttcag tccaagccag accggtaaca
     3241 acaccagcaa tatcgttacc ttggtaaagt tcctttgtat agcgcggaac tccaagaatc
     3301 ttttcaatat ccttattggt tagcttggga ttatactttt gctccgaggc aatcatacgg
     3361 gcagcatagc gaaccaactt tgcaattttc tggttcaggt ttcgaacgcc gctctcccgg
     3421 gtgtattcct ggattacctt ggccaatact ttatcgctca actccaaggc atcactattc
     3481 aagccatgct ccttgagttg gttgggcagc aaatggcgct tagcaatctc gaccttttcc
     3541 tccaagctgt acccgctaac ctcaatcatt tccatacggt cgcgcagcgc aggatttatg
     3601 gtagccacgg tattggcagt ggtaataaac agcacctttg aaagatcata ttccacctcc
     3661 acaaagttat cgtggaatgt gctattctgc tcggggtcga gcacttccag caaagccgag
     3721 gcgggatcgc catggaaatc tttgcccacc ttatcaatct catcgaggat gatgacagga
     3781 tttgacgatt ttaccttttt caggctttga attatgcggc cgggcatggc gccaatgtac
     3841 gttttgcggt gaccacgaat ctcagcctca tcgtgcatac ctcctaacga gatacgagca
     3901 tactttcgac ccaatgctct ggcaaccgac ttgccaagcg aggttttacc cactcccgga
     3961 ggtccgtata agcataggat tggcgccttt aggtcgcctt taagtttgag cacagccaaa
     4021 tgctccagga tacgctcctt tacctgctct aggccaaagt ggtcttcatc gaggatattt
     4081 ttggcatgat caaggttgaa attgtcattg gtgtactcat tccacggcaa gtcgagcagt
     4141 agctgaacgt agttcagctg aaccgaatac tccccggcca tggggttcat acggttgagt
     4201 ttgttgacct ccttatcaaa atgttcagca acttccttac tccacttttt ggccttagcg
     4261 cgctccttga gctcatttat ctcctgctca atggggtttc ctcccagctc atcctgaatg
     4321 gttttcatct gctgctgaag gaaatactca cgctgctgct ggtcaagatc gtgcttaacc
     4381 ttgttctgaa tatcgtgttt caactcaagc atttgcagct cacgggttaa tattcgcagc
     4441 agctgctcag cccgtctctt aaggtcgtgc tcttcaagca atgcttgttt ttcatcagtc
     4501 ttaatatcgc tatttgccga tataaagttc acaagcattt tggggttatc aatattcttt
     4561 atggcaaacg aagcctcctt gggtatattt ggcgaaagat gtattattcg aatggtgaga
     4621 tcccttattg attcggttaa agctatgaac tctgtatcgt cctcgtttgg taaaagctca
     4681 gccagttcca gaacgcgagc cataaagtat ggctcctcac ttacaaattg gtcgacacgg
     4741 aatcgcgata atccctgaag tataactgac gagttgccat cgggtagctc aagcacctta
     4801 ataacctgag caatggtgcc aaccttaaaa agatcgcctg atgagggatc ctcaatacgg
     4861 gcatcgactt gcgagagcac cccaattatt ttcttctttg gtggtagact tgcaattagg
     4921 tttagcgatt tctcccgccc aacggtaatt ggcatcaata caccgggaaa gagaacagtg
     4981 ttacgcaatg ccattatggg tagcacatcg gggatatctg cctttttgac ctcgggcact
     5041 tcgccctctg ccaaaatggg tataaagttt acttctgaat cgttagtatc gtccatttgc
     5101 tgatttagta ttgatagtcc aaactttttc ttcattttat ctcctctcaa ttttctgaca
     5161 atatgacaca ttaaagggta aaactaaccc agcttactat atggtcaatt gctatgccaa
     5221 aaagtagttg atggttgttg gtattaaaac cttgaacccc tcagagcgga acaggccatt
     5281 aaaccctgaa tatctaccct ttatccttta tcctttaacc tttacccttt atcctttacc
     5341 ctttatcctt aatacatcct gtattctcgg gttagctcaa aaaccttaag taggatttcc
     5401 ttgtcgatat cggtaaatgg caaatcgcga cgcgacatca taatccgggc ggtttcaaaa
     5461 gctttttggg gttgataggt tgtaaagccg gctgcccctc cccacgaaaa gcaagggata
     5521 aaatttcggg ggaaaccatc gccaaaaatg ttggcactta caccaaccac tgtgccggta
     5581 ttaaacatag tatttatgcc acattttgaa tggtcgccca ttattagccc acagaactgc
     5641 aatccggtat caacaaatcc cttttttcgg tagctccaaa gctttacaga agcgtaattg
     5701 tttttgaggt ttgagttatt ggtatcggca cccagattac accattcgcc tattaccgaa
     5761 ttaccgataa aaccatcgtg agccttgttt gaaaaagcaa aaaacacgct gttattaacc
     5821 tcgccgccaa ccttgcagta tggacctaca gtggtagggc catatatttt tgcacccatt
     5881 ttcagcgctg aatgctcacc aagggcaaac gggccacgca ctatggaacc ctccattact
     5941 tcggcatcct tccctatgta tatcggacca gtttcggcat taagaattgc gcactccacg
     6001 cgagcaccct cctcaataaa aacattatca gggtttaaaa cagtattggt agctgaaatt
     6061 ggttgcgact tccgcccctt agtgataagg ctataatcgc gaacaatggc ttcgccattg
     6121 tttcggaaaa tatcccaggg atacttgacc tcgagaatat catccggata ctcaaacgtc
     6181 ttctcaaaca agcccgctgc gggtaaagtg tccactgtgc tattaagcat tgccgctata
     6241 agcgtgtcgc ccttaacaat tgcctggtta ggtttgagcg ctcttatttt atcaaccaaa
     6301 ttgctatcgg ggcaaatacc tccatttacc aacaaaacag gttccccttt gggggcattg
     6361 ggatatttgg tctgaaggta atcctgcgtt aaatagtgaa ccgatgcccc ggttaacatt
     6421 gaccactttt gggatatggt aagaataccc atgcggattt cagccaccgg acgggtaaag
     6481 gttaaaggca gtaaatcgtc gcgcctttgg tcatcaaaaa gaactatggt gttaatcata
     6541 taaatcgatt tattttgaac aaagttacca aaaaaagtaa cccttttaaa acaaaaaaag
     6601 ctcttatacc taagagcttt tcctgaatta tttgagcaac attacttttt gttatccatg
     6661 tgctttttgt agcggttcat gaacttatcc acacgaccgg cggtgtcaac cagcttaacc
     6721 ttaccggtgt agaatgggtg tgaagtgttc gaaatttcaa cctttaccaa aggatactca
     6781 actccatcga tagtaatggt gtccttggtg ttaacagccg acttggtgat aaatacatgc
     6841 tcattggaca tgtccttgaa tgccaccaga cggtaatttt ccggatgtat tccctttttc
     6901 attgtattat aaatttgttt acttccaact tcggtttaat gggctgcaaa gttatcaact
     6961 tatatttaaa tatcaaacta ttgcagctaa aaaatgattt atccggataa gttttaattt
     7021 cgcctgttca gtttggcaag cagtttaaga atctcaatgt aaagccagat aagggtaacc
     7081 atcaagccaa atgctccata ccactccatg aacttgggtg cacccgatgc tgaagcccta
     7141 tcaataaaat cgagatcgat aatgatatta agcgctgcaa caccaactac tattaggcta
     7201 aagatgattc caaaggttcc gctaccatga ataaaaccca agcttatgcc aaagaaactg
     7261 agtacaaagg aaataaagta ggttaatgca atagcgccag tggctgctaa aaccatcacc
     7321 ttaaattttc ctgtagctct gattgttccg ctacggtaag ccaatagcat caggaagaaa
     7381 acggctaggg ttaacccaat tgcctgcatt attataccag caccatacga tgatgcaaag
     7441 taggccgata ttccaccaag gaaaatcccc tcaagcaagg cataaaccgg agcagtgtaa
     7501 ggtgaagttt tgggcgcaaa tatcaccacc aacgatgtaa taaaaccgcc tattcctcca
     7561 acaaacatcc acggaacaac cgatgcaggg ttgacactct cgaaaaagat acgccaggta
     7621 taagctgctc ccagaattac cagcgcaagc atcagcaatg ccttattcaa agttccctga
     7681 agtgtcataa cctcagcacc cgaagcataa ccagcttttg aaaatatcct gtccgatagg
     7741 gcaggatttg acgatttagt taagttcata caaatacctt gtttaaaatt caatacgcat
     7801 tattcaaaaa ttatgccgtg caaaagtagc agattattcg gttaattcaa aaagtagctc
     7861 attaccaacc catgcagcca gctcgttctc ggactcggtc atcctactgg ctgtttccga
     7921 aacccttgca gccagttcag gactgcgttt agcaatttcg cgtaaggcga actgaactgc
     7981 cgactgggtt aaatcggtca tctcctcggc catattctca atgatgccaa tataaggttc
     8041 aagcaaactc tctttaatgg ctgtatcgga tgccttacgt gcaataatta gcaaacctgc
     8101 tttacgcaaa agctcatcgt tgcttaggca ccattcaatg ctacgcgaaa gcgcaaattc
     8161 cgatttccag aacaagttac cgcaaacctg ttccacaatt tcggggttgg taaactccgc
     8221 agcccactca tcaatctgct cacctgtcaa tctttcaggg tcggcaatca ggcttgccag
     8281 tattttacac tccctgatat cttcggcata aagttctaac gccaggtcat ggtcgccctt
     8341 atagggcttt gccaactcct tgagttcagg aataggaacg ccatagttta ctttgtaaat
     8401 aataccggcc atacgcatgt tctctgccac ttcgccacta gccctgtgta ttatctgcga
     8461 ttttatctcc aagacctttt ccaacgttgc cataagtttt ccaattttgc tcaaaattac
     8521 actgaaaaag tgcagattca aagctttggg ggaaatagtt cagcctaagt gccatgaatt
     8581 ttgaggaatg ggtcggaatg aatcggaatg agtcggatta gacgaatatg atgaagttag
     8641 aggaagttta agttcgttaa acgtgaaaag tgaaaagtta aaagaagaat tgagcagata
     8701 aataaataca tcaggagtcc gacaaaagtt tttaggaacc aaaaactcgt acttattcaa
     8761 ttaccgcgta gcggttagac tgttgtaata gaatagaaag gagcatactt tatattcctc
     8821 ttataagata aaggttttta atagaaaatc aatgtcgttt agttaacaaa aatattcatg
     8881 cctgtcttcc tgagcggcct gtcccgccct agcggggagc gaaggatcta caatcgaccc
     8941 attgagacgt ttcgcttgcg ctcaacatgg caatagttcc acgaacaacg aacaacgctt
     9001 ccagccccgt aggggcgtaa tatttgtaac tccatggcta ctccctcatg tgttgcgatg
     9061 cacgcaatac attttccaaa gagcaagggg gaagcgttgc atcgcaacgg aaaagcatta
     9121 caaagagtta tttaaacgtt ctttcttg
//
LOCUS       DAOOWJ010000102         9034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_7667_length_9034_cov_7.953558, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000102 DAOOWJ010000000
VERSION     DAOOWJ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7667_length_9034_cov_7.953558"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLW22_09465"
     CDS             <1..203
                     /locus_tag="PLW22_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOU74969.1"
                     /translation="NLITPEWRLFLAVGFCGGFTTFSSLANDAFILLQGREFLYLSAY
                     TAASFFLGLLAIFAGRYIIQII"
     gene            208..567
                     /locus_tag="PLW22_09470"
     CDS             208..567
                     /locus_tag="PLW22_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOU74970.1"
                     /translation="MENFSKALRLRIYISSTDKLKHSLLFETIVYQAKRYGLAGATVY
                     KGIMGYGASSVIHSYKFWEITEKLPVTVEVVDLEENIRRFYDKVKPFLEDMRYGCMVT
                     AEPIEVWLYKEGSKRDY"
     gene            730..2076
                     /gene="tig"
                     /locus_tag="PLW22_09475"
     CDS             730..2076
                     /gene="tig"
                     /locus_tag="PLW22_09475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOU74971.1"
                     /translation="MNIVREDIDSLNTTIRVKVEKNDYAESVETSLRNYRKKANINGF
                     RPGMAPMSLIRKMYYRYVIADEVTKLASDKLFEFIKENKIRTLGEPIPSEKQTPINWE
                     TDETFELAWDLGIAPEIDIKFTKKDKFPFYKILPNDEIRKSYIDNYRNRNGKYVSVDV
                     TDDKGLIKATLTELNDDESPRENGITVEGASISVALVADEAERKKLTGVKVGDVIVID
                     THKAFPNQADRSALFRVKKEELENVNPLFQITVTETLTFAPAELNQEFFDAVYGEGVV
                     NSEEEFHKKIEEEIERNLAYESEYRFGIDLKEKLLEKLKIELPKEFLIRWLVAINEGK
                     FTREQIENEFPMFEKDLKWQLISNKVAEEQNFTVTNEELEDFAMGYARSQFAAYGMSF
                     LPDEYIKRYATNILKNRDEVRKMQERILDKKVIEWFKANVNLDTKEITIDEFNKLK"
     gene            2212..2880
                     /gene="clpP"
                     /locus_tag="PLW22_09480"
     CDS             2212..2880
                     /gene="clpP"
                     /locus_tag="PLW22_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOU74972.1"
                     /translation="MREFEKYAIKHRGINSLTLHQYTSMSNGYISPTIIEERQLNIAT
                     MDVFSRLMMDRIIFLGVPINDDVANIIQAQLLFLESTDPSKDIQIYINSPGGSVYAGL
                     GIYDTMQYISSDVATICTGMAASMAAVLLTAGEKGKRTALPHSRVMIHQPMGGAEGQA
                     SDIEIVTREILKLREELYEIIATHSGNPIERIRKDSDRDYWMTAQEALEYGMIDEVLM
                     RNKK"
     gene            complement(2934..3716)
                     /locus_tag="PLW22_09485"
     CDS             complement(2934..3716)
                     /locus_tag="PLW22_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU74973.1"
                     /translation="MKRLIISAFMIVFTNASISQVSEMYKAEIFNSKGIQLPYRILYP
                     KNFNSTQKYPLVLFLHGSGERGSDNVKQLTHGAKLFASDSVMDKYPAIVVFPQCPAES
                     YWANVKIVNQSDGNRSFDFPAKGKPTPAMAAVIELLDSIVKLSYIDRNRLYVGGLSMG
                     GMGTFELIARKPKMFTAAFPICGGGSPKNVKRYAKRVKIWVFHGEADDVVPLYHSQRM
                     VDAIKQAKGDVRFTVYPGVGHNSWDNAFAEPELLPWLFSKSK"
     gene            complement(3917..4615)
                     /gene="radC"
                     /locus_tag="PLW22_09490"
     CDS             complement(3917..4615)
                     /gene="radC"
                     /locus_tag="PLW22_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOU74974.1"
                     /translation="MENSEKRLTIKDLAEDDRPREKMERKGASSLSDAELIAILLRAG
                     NASETAVDVAQRLLGQVNNSLNELARLSLHQFTKIKGIGRTKAITILAALELGRRRSA
                     EGPVNRERIFSSRSVIDIFQPALADLPHEEFWVLLLNKANRIIERVKVSQGGVGGTVV
                     DPKLVLKHAVERLASAIIVVHNHPSGNPQPSDKDIALTEKLKNAAQLFDITLLDHVIV
                     TDGECYSFADNGKM"
     gene            4731..5534
                     /locus_tag="PLW22_09495"
     CDS             4731..5534
                     /locus_tag="PLW22_09495"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HOU74975.1"
                     /translation="MQSKNLIRSALLVAIVALAYYTYTQIVANSFNNLTFSADSVVFK
                     RGCLHPDSSCFRVVYRFPVTVGKGSSRIEKLIVSDFVGNMDEEIDRNDFAHLKDMLVK
                     NSISYDSSYVEFLKQFGTGVEWYIDVDFKQILRTNRLITFRYEQSSYTGGAHDMYGYH
                     YLNIDLKKSKILKLTDLFTDMDRFKAIAQKAFTVQYLNNPADKTEYWLADDGSLVLAN
                     EFGLTSKGLLLHYNVYEIACFARGDIELLIPYPDLKEILVDAYRKELMN"
     gene            complement(5621..7120)
                     /gene="hutH"
                     /locus_tag="PLW22_09500"
     CDS             complement(5621..7120)
                     /gene="hutH"
                     /locus_tag="PLW22_09500"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOU74976.1"
                     /translation="MAKVHTISPKPLSWDVIENLIDEPHTLELSEESRQLIAKCRDYL
                     DRKMENEETPIYGITTGFGSLCNKTISKDELSQLQKNLVMSHACSTGDMVKPEIVKLM
                     LFLKAHALSLGHSGVQVETVQRIIDFYNNDVIPVVYDRGSLGASGDLAPLSNLFLPLI
                     GMGEVHYDGQIVSAADVLKKFGWKPIELKSKEGLALLNGTQFMSAHGVYAMVKAFKLA
                     KLADIIGAVSLEAFDGRIDPFHENIQNVRPHQGQIETARAFRKILDGSELIKRPKKHV
                     QDPYSFRCIPQVHGASKDAINYVAGVLLTEINSVTDNPTIWPDEDLVISGGNFHGQPL
                     ALAYDFLAIALAELGNISERRTAQLILGLRGLPEFLVANPGLNSGFMIPQYAAAAMVS
                     QNKQLCAPASVDSIVSSNGQEDHVSMGANAATKLLRVVDNLERILAIELFNAAQALEF
                     RRPAKSSPYIEEFIASYRSIVGFIEQDRIMYKEMDKTVEFLRQGKFQRE"
     gene            complement(7138..8514)
                     /gene="hisS"
                     /locus_tag="PLW22_09505"
     CDS             complement(7138..8514)
                     /gene="hisS"
                     /locus_tag="PLW22_09505"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOU74977.1"
                     /translation="MAKTKPSIPKGTRDFGPDQMVRRNYIFDTIRTVFSKYGYSPIET
                     PAMENLSTLLGKYGDEGDKLLFKILNSGDFIDKVEPSLLKPGNSNAASLAICEKGLRY
                     DLTVPFARFVVQYQNEITFPFKRYQIQPVWRADRPQKGRYREFYQCDVDVIGSNSLLN
                     EAELVLIINEVFGKLNVRTLLKVNNRKILSGIAEVIGHADKLVDITVAIDKIEKIGID
                     AVNAELRERGIDEVAIQKLQPIIQLQGSNAQKLSTLRNVLQGSPIGQKGIDEMEELFR
                     YIEAWGSLSTQVELDLSLARGLNYYTGAIFEVKAQDAEIGSICGGGRYDDLTGIFGLP
                     NVSGVGVSFGADRIYDVMQQLNLFPESLTSTTQVMFANFGTTEVAYCMPAARKLRDAG
                     ISVEIYPDAAKVKKQFEYANKRNIRFVVIAGETEIQNRQFSVKNMATGNQVTIPFDGI
                     EEHIKLNL"
BASE COUNT         2577 a         2040 c         1970 g         2447 t
ORIGIN      
        1 gcaacctaat cacgcctgag tggcgtttgt tcttggcagt gggcttttgt gggggattca
       61 ccaccttttc atcattggca aacgatgcct tcatactgct tcaaggccgc gaatttttgt
      121 atctttctgc ttacacggca gccagcttct ttttgggcct gttggcaatt tttgccggtc
      181 gctacatcat ccaaattatc taacactatg gaaaatttca gtaaagctct acgactacgc
      241 atctacatta gctcaaccga taagctgaag cattcgcttc tttttgaaac cattgtttac
      301 caagcaaagc gatatggctt ggctggtgcc accgtttata aaggaattat ggggtatggc
      361 gcaagttcgg taatccactc atacaaattt tgggaaatta ctgagaaatt gccggttacc
      421 gttgaagtgg ttgacctgga ggaaaatatc cgcagattct acgataaagt taagcctttt
      481 cttgaggata tgcgatacgg ctgtatggtt actgcagaac ccattgaggt gtggctctac
      541 aaggagggtt caaaacgcga ttactaagct tccatccgct cagtgctaac aatcacccgg
      601 tcattacagt taaacttcgt ttagtagctt gctgattatt ccactattag ctacctttgt
      661 ggacttaatt ttaacatagt tactgacgta aagtataata aatcaagcaa ctaaatcaat
      721 tcacatagta tgaatattgt tagggaagat atcgattcgt taaacacaac catacgggtt
      781 aaggttgaga aaaacgatta cgctgaaagt gttgagacta gcctgaggaa ttaccgcaaa
      841 aaggcaaaca tcaatggttt ccgtccggga atggccccta tgtcgcttat tcgcaagatg
      901 tactaccgct atgtcatagc cgatgaggta accaagctgg cctcggataa actttttgaa
      961 tttattaagg aaaataagat ccgaaccctt ggcgaaccca ttccaagcga aaaacaaacc
     1021 cctattaact gggaaaccga cgaaaccttt gaactcgcct gggacttagg tattgcccct
     1081 gaaattgata tcaagtttac caaaaaggac aaattcccat tctacaagat cttaccaaac
     1141 gacgagatac gcaagagtta catcgacaac taccgcaacc gcaacggcaa gtatgtaagt
     1201 gttgatgtta ccgatgataa ggggctgatt aaggctaccc ttaccgaact caacgacgac
     1261 gaatcgccac gcgaaaacgg cataaccgtt gagggtgcgt ctatttccgt tgcccttgtt
     1321 gccgatgagg ctgaacgcaa aaagctcacc ggtgttaagg ttggcgatgt gattgttatt
     1381 gatacccaca aggcattccc caaccaagcc gacaggtcgg cgctattccg agttaagaag
     1441 gaggaactgg aaaacgttaa tcccctattc cagataacgg ttactgaaac attaaccttt
     1501 gccccagcag agcttaacca agagtttttt gatgcggttt acggcgaggg tgttgttaac
     1561 agcgaggaag aatttcacaa gaaaattgag gaggagattg aacgcaacct tgcctatgaa
     1621 agcgaatacc gctttggtat cgacctaaag gaaaagctgc tcgagaaact taaaattgaa
     1681 cttcccaagg aattcctaat ccgctggttg gttgccatca acgagggcaa gtttacccgt
     1741 gagcagattg aaaacgagtt ccccatgttt gaaaaagacc ttaagtggca gctcatcagc
     1801 aataaggttg ccgaagagca gaactttacc gttactaacg aagaacttga ggattttgcc
     1861 atgggatacg ctcgcagcca gtttgctgcc tacggcatga gcttcctacc cgatgaatac
     1921 atcaagcgtt acgcaaccaa tatcctcaag aaccgcgacg aggttcgtaa aatgcaggag
     1981 cgcatactgg acaaaaaggt tattgaatgg ttcaaggcta acgtaaacct ggacaccaag
     2041 gagataacca ttgatgagtt caacaagctt aagtaataaa gcaaaacata gcatagccgg
     2101 tttgtaaacc ggctttttat tttttggttt gttagtaaat tgtagccact ccgctttttt
     2161 gttttaacaa atgtgagtat tttgcgttac taatacaaaa aattaaaggc tatgagagaa
     2221 tttgaaaaat atgcaataaa acaccggggt attaacagcc taaccctaca tcagtataca
     2281 tcgatgtcaa acggctacat ctcaccaacc atcattgagg agcgccagct caacattgcc
     2341 accatggacg tgttctcgcg ccttatgatg gacaggatta ttttcctggg tgttcccatt
     2401 aacgacgatg tagctaacat cattcaagca cagctgctat tccttgaatc gaccgatcca
     2461 tcgaaggata ttcaaatcta cataaactca cccggtggga gtgtttacgc tggcttggga
     2521 atttatgaca ccatgcagta cataagctcc gacgtggcaa ccatctgtac cggcatggct
     2581 gcatcaatgg cagctgttct gcttaccgca ggcgaaaagg ggaaacgaac cgccctaccc
     2641 cactcacgtg taatgattca ccagcccatg ggtggagctg aggggcaagc ttccgatatc
     2701 gagattgtta cccgcgaaat acttaagctc cgcgaggaac tttacgagat tattgccacc
     2761 cactcaggca atcccattga acgtatccgt aaggactccg accgtgacta ctggatgacc
     2821 gcacaggaag cacttgaata cggtatgatt gacgaagtgc ttatgcgtaa taagaagtaa
     2881 tttggaaccc gaaaaaaatt aaaggctgca ttttatgcag ccttttttag tatctacttg
     2941 cttttactaa acaaccaggg taacagctct ggttcagcaa aggcattatc ccagctatta
     3001 tgcccaactc caggataaac cgtaaagcgc acatcgccct tagcctgttt aatggcatcg
     3061 accatgcgct gggaatggta taggggaaca acatcgtcgg cctcgccgtg aaatacccaa
     3121 attttaaccc ttttggcata tctttttaca tttttaggac taccaccacc acaaatggga
     3181 aatgcagcag taaacatttt aggctttcgg gcaatcagct caaaggtacc cattcctccc
     3241 attgatagtc cgccaacgta taacctattc ctatcaatat agctcaactt tacgattgaa
     3301 tccagaagtt caataacggc agccattgct ggggttggct tacctttagc cggaaaatca
     3361 aaggaccgat taccatcgga ttgatttaca atttttacat tagcccagta gctctcagca
     3421 ggacattgcg gaaaaactac aattgcgggg tatttatcca taacagaatc agatgcaaac
     3481 agcttagccc catgggtaag ctgcttaaca ttatcggaac cccgttcgcc agagccatgc
     3541 aggaaaagaa ctaatggata cttttgggta gaattaaagt ttttaggata cagaatacgg
     3601 tagggcaact gaataccttt agaattgaat atctccgcct tatacatttc actaacctgc
     3661 gaaattgagg catttgtaaa tacaatcatg aaagcagata tgattaacct tttcatttcc
     3721 tcaaaattta taatgggtaa agttagtgaa tcatagttta aaatgtgctg ttttcagtag
     3781 taattggtta ttagtaacca tcaacaaaaa aagtatctca aagcgacaag gtgttcatac
     3841 agtgtttaca aggtgtatgg gtgtaaatgt agtagcatcc acaaacaacg attaacaatt
     3901 aaccagcaga taatatttac atttttccgt tatcggcaaa agagtagcat tcgccatcgg
     3961 ttactatcac gtgatcgagc agggtgatgt caaatagctg cgcagcattc ttgagctttt
     4021 cggttagtgc aatatcctta tcgctgggct gggggttacc cgatggatga ttgtgaacca
     4081 ctattatagc cgaagcaagc cgctcaacgg cgtgttttag caccagctta gggtcaacca
     4141 ctgtgcctcc cactccgcct tggctcacct ttaccctttc aattatccta ttggccttat
     4201 tcagaagcaa aacccaaaac tcctcgtgtg gtaaatcggc aagcgcaggt tgaaatatat
     4261 cgattacgct ccggctcgaa aaaatccttt ctcggtttac tggtccctca gcactccgcc
     4321 tgcgtcctag ctcaagcgca gccagtatgg taatcgcttt ggttctgcca atccccttta
     4381 ttttggtaaa ctgatgcagg ctcagacgag caagctcgtt gagactatta ttcacctgcc
     4441 caagtagccg ttgggcaaca tcaaccgctg tctcacttgc attgccagct ctaagcagaa
     4501 tagctatcag ttccgcatcg ctaaggcttg aggctccttt tcgctccatt ttctcgcgtg
     4561 gacggtcgtc ctcagccaaa tcttttatgg ttaaccgctt ttcggagttt tccatgacca
     4621 tggctttgaa taggttacat aagttaaaca gaacggaaat aaaaagcaag cagttttatc
     4681 aaaatcaata taaatagcaa tatatttgat gttactaatc gaagaacgct atgcaaagta
     4741 agaacctgat tcgctcagcg ctgctggtgg caattgttgc cctggcttac tatacctaca
     4801 cccaaattgt ggcaaatagt ttcaacaatt taaccttttc ggccgactcg gttgttttca
     4861 aacgcggttg cctacatccc gactcatcgt gttttagggt tgtttaccgt ttccctgtta
     4921 ccgttggtaa gggtagctcc cgtattgaaa agctaattgt aagcgatttt gtaggaaaca
     4981 tggatgagga gattgataga aacgattttg ctcacctaaa ggatatgctg gtaaaaaact
     5041 ccataagcta cgacagttcc tacgtagagt tcctgaaaca atttggtact ggcgttgagt
     5101 ggtacattga tgttgatttt aaacaaatat tacgtacaaa tcgtttgatt actttccgtt
     5161 acgagcaatc gagttacact ggcggtgccc acgatatgta tggttaccat tacctgaata
     5221 tcgacctaaa aaaatcaaaa atcctaaaat tgaccgacct gtttacggat atggaccgct
     5281 tcaaagccat tgcccaaaag gcctttacgg tgcagtacct gaataatcct gccgacaaaa
     5341 ccgaatactg gttggccgac gatggctctt tggttcttgc caatgagttt ggtttaacca
     5401 gtaagggttt gctgctccat tacaatgttt acgagatagc ttgctttgct cgcggcgata
     5461 ttgagctgtt aatcccctac cctgatttaa aagagatttt ggttgatgct taccgaaagg
     5521 aattgatgaa ctaaaaccca agtctttttt acaatagctt aactaaagaa aaaaccgaca
     5581 gccaaaacag ctgtcggtta tttttttaag gaaatcttat ttactcccgc tgaaacttac
     5641 cctgtctgag gaattcaacg gttttgtcca tctccttata cattatccta tcctgctcaa
     5701 taaaacctac tatggaacgg tagctggcaa taaattcctc aatgtaaggc gatgattttg
     5761 caggcctacg gaactcaaga gcttgggctg cattgaatag ctcaatggcc agtatgcgct
     5821 caaggttatc gaccacccta agcagctttg tggctgcatt ggcacccatg ctaacatggt
     5881 cttcctggcc gtttgaggaa acaatggaat cgaccgatgc gggagcacaa agctgcttgt
     5941 tctggcttac catggctgca gcagcatact gtggtatcat aaaaccggag tttagtccgg
     6001 gattagcaac cagaaattca ggtaaaccgc gtaagccaag aataagctgc gctgttcggc
     6061 gttccgagat gttgccaagc tctgcaaggg caatggcaag gaaatcgtat gctaaggcca
     6121 gcggctgccc atggaaatta ccacccgaaa ttaccaaatc ctcatcgggc caaatggttg
     6181 ggttatcagt aaccgagttt atctcggtta gcaatactcc tgccacatag ttaatggcat
     6241 cctttgaggc tccgtgcacc tgcggaatac accggaatga gtagggatcc tgaacgtgct
     6301 ttttggggcg cttaatgagc tcgcttccat caagtatttt tcggaaagca cgggccgttt
     6361 caatttgtcc ctgatgcgga cgaacattct gaatattttc atggaatggg tcaatacgac
     6421 catcgaacgc ctcaagtgaa actgcgccta ttatatcggc tagcttggcc agcttaaaag
     6481 ccttaaccat ggcgtaaacg ccatgtgcac tcataaactg cgtgccatta agcagggcaa
     6541 gcccttcctt cgacttcagt tcaatgggct tccagccaaa ttttttgagt acatcggcag
     6601 ccgaaactat ttggccatcg taatgcacct cacccatacc aattagcggc aggaacaggt
     6661 ttgaaagggg cgccaaatcg cccgatgctc ctagtgagcc acggtcgtaa acaacgggaa
     6721 ttacatcgtt attgtagaaa tcgataatac gctgaacggt ttcaacctga acacccgaat
     6781 gtcctaacga tagtgcgtga gccttaagaa agagcatcag ctttacaatt tcgggcttaa
     6841 ccatatcgcc tgtgctgcag gcatgcgaca taacaagatt tttctgcagc tgtgagagtt
     6901 catccttcga tatggttttg ttgcatagtg aaccaaaacc agtggtaata ccgtaaatag
     6961 gggtctcctc attctccatc ttacggtcaa ggtaatcgcg gcacttggct ataagctggc
     7021 gcgattcctc ggaaagctca agggtatgag gctcgtcaat caaattttca attacatccc
     7081 agcttaaggg cttgggcgat atggtatgaa cttttgccat ggttaatggt ggttttttta
     7141 taggttcaac ttgatatgtt cttcaatgcc atcaaaagga atggtaacct gattgccggt
     7201 tgccatgttt ttaaccgaaa actgacggtt ttgaatttcg gtttcacctg caattaccac
     7261 aaagcgaatg ttgcgcttgt ttgcatactc aaactgcttt ttcaccttag cagcatcggg
     7321 gtaaatctca acgctaattc cggcatcgcg gagcttacgg gctgcaggca tgcagtatgc
     7381 aacctcagtt gtcccaaagt ttgcaaacat tacctgggtg gtggaggtaa gactctccgg
     7441 gaaaagattt aactgctgca ttacatcgta aatgcggtcg gcgccaaacg aaacccctac
     7501 cccgctcaca ttgggcaaac caaaaatacc ggttagatcg tcgtaccggc caccgccaca
     7561 aatgctacca atctcagcat cctgggcttt tacctcaaat atggcaccgg tgtagtagtt
     7621 aagccctcgc gcaagcgaaa ggtcgagttc cacctgtgtt gataagctcc cccaagcctc
     7681 aatgtatcgg aaaagctcct ccatttcatc aattcccttc tggcctatgg ggctgccctg
     7741 caaaacattg cgtaaggtgg aaagtttttg ggcgttgctg ccctgtaact gaattatggg
     7801 ttgcaatttc tgaatggcaa cctcatcgat tccgcgctca cgcaattcgg cgttaacagc
     7861 atcaatacct atcttctcaa tcttatcaat tgctacagta atatcaacca gcttatcggc
     7921 atgaccaata acctcggcta ttccgcttag tattttgcgg ttatttacct tgagcagggt
     7981 gcgcacgttg agctttccaa aaacctcatt gataattagc accagctcag cctcattaag
     8041 caacgagttt gaaccaataa catcaacatc gcactggtag aactcgcggt aacgaccctt
     8101 ttgtggcctg tcggcacgcc aaacgggctg tatttggtat cgtttaaagg ggaaagtaat
     8161 ctcgttctgg tactgcacca caaatcgggc aaaaggaaca gtaaggtcgt aacgcaaacc
     8221 cttttcgcat attgctagcg atgcggcatt tgagtttcct ggtttcagta ggcttggctc
     8281 aaccttatcg ataaaatcgc ctgagttaag aattttgaaa agaagcttgt cgccttcgtc
     8341 gccatacttg ccaagtaggg ttgaaaggtt ctccatggca ggcgtttcga ttggcgaata
     8401 accatacttg ctaaaaactg tacgaatggt atcgaaaata taattcctcc gaaccatttg
     8461 atcaggacca aaatcgcgag ttcccttggg aatgcttggt tttgtctttg ccattgtgtt
     8521 gctgtttgaa acgactgcaa acctacaatt tttttgggag aaaaaggcaa atgaaggtta
     8581 aaggaagtgc atgaatcgtt aaccgtgagc cgtgagccgt taaacgagtt ttttagagaa
     8641 tgtcaggaag ttagaggagg ttagaagaag tgcatgaatc gttaaccgtg aaccgttaaa
     8701 cggaatctaa ttattaccta ttgtcattcc gagtcccgat atatcgggat agactatgcc
     8761 gaggaatctc gattgattta aaacgtgcac cgtgaaacgt gagccgtgag ccgttaaacg
     8821 aaatctaata tcacctattg tcattcggag ctcgtcgagg aatctcaatt gaactaaaac
     8881 gttaaccgtg agacgtgtga cgttaaacga gttttttaga gagtgtctgg aagttagagg
     8941 aagttagagg aagttagaag aaatgcatga atcgttaacc gtgagccgtg aaccgttaaa
     9001 cgaaatctaa tatcaccatt tgtcattccg agtc
//
LOCUS       DAOOWJ010000103         9005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_7699_length_9005_cov_7.385028, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000103 DAOOWJ010000000
VERSION     DAOOWJ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7699_length_9005_cov_7.385028"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..470
                     /locus_tag="PLW22_09510"
     CDS             6..470
                     /locus_tag="PLW22_09510"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOU74978.1"
                     /translation="MNKYKTIALVAHDNRKRDLVEWVEWNWAKLLKHKLICTGTTGRL
                     VEEALTKMNNDAGNHLQLTRLKSGPLGGDQQLGALIAEGKIDILIFFWDPMQAQPHDV
                     DVKALLRIATVYNIPTATNRSTADFLISSPLIEQEYTPKLKDYSQYINRDVY"
     gene            483..767
                     /locus_tag="PLW22_09515"
     CDS             483..767
                     /locus_tag="PLW22_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74979.1"
                     /translation="MERNEYRENVKKAIDEIFDQIEALRAKADNASDKVRQEYNEKIK
                     ELEMLRNQMESKLDELAQAADSKWDEVKDTVNSSLESFKEGFKKLGSLFD"
     gene            786..983
                     /locus_tag="PLW22_09520"
     CDS             786..983
                     /locus_tag="PLW22_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74980.1"
                     /translation="MNAFELSLIVGIVLLLIAWIFVLTDILRNRFPERNMWIIYLIIT
                     PPLAVLVYPIVRERLLRNARK"
     gene            complement(996..1439)
                     /locus_tag="PLW22_09525"
     CDS             complement(996..1439)
                     /locus_tag="PLW22_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74981.1"
                     /translation="MSEKNYDPRMHTAEHILNQTMVRLYNCGRAFSSHVEKKKSKCDY
                     RIPRALTDEELKTIESRVNEVISQNLNVTEEFTDKSNASTFLDLSKLPADAPESIRVI
                     RVGNYDACACIGPHVANTKEIGRFKIISADYSDGTVRIRFKLENE"
     gene            1575..2468
                     /locus_tag="PLW22_09530"
     CDS             1575..2468
                     /locus_tag="PLW22_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU74982.1"
                     /translation="MFDLSKKRWQWAAMLFMAFVWGASFILMKRGLDALSYVQVAALR
                     IFFSFLILLPLSIKNLKLINRQNFWPLLGCGLLGNFFPAFLFTLSETQISSSLAGFLN
                     GLTPFFTVIAGLLLFKHKPNAFQSIGVLIGFVGASILITNGNFSSFGHVNLYALLVVL
                     ATMMYGINSNIIRFKLSGLTGVQITSLIFFMIGIPAGIILISTDIGAAMQHPAFATSI
                     LATIVLATFGSVITLFVYNNLIHHTSAIFASSVTYIIPFFALLWGILDGDHIGALQIV
                     SLVIILIGVYLSSLRRKPKLL"
     gene            2572..3474
                     /gene="ppk2"
                     /locus_tag="PLW22_09535"
     CDS             2572..3474
                     /gene="ppk2"
                     /locus_tag="PLW22_09535"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOU74983.1"
                     /translation="MTKDKKDKKKKQADKPKETKKTKKHHLPDLGNDHYETVEAHVDR
                     MDKKTYEHEIEKLHVELVKLLEWIKHQKLKVVLLFEGRDAAGKGGVIKTIAERLNPRF
                     CRIVALGVPTEKEKTQWYFQRYVAHLPAAGEMVLFDRSWYNRAGVEKVMGFCSEEEYT
                     EFLRSCPEFERMLVRSGIILIKYWFSVSDKEQERRFQERIDDPTKRWKLSPMDIESRN
                     RWVEYSKAKDVMFAHTDIKQAPWWVVRADDKRRARLNCIHHLLSLIPYEDLTPPPVKL
                     EPRRIDESYVRPPITDQNFVPEIY"
     gene            complement(3554..3946)
                     /locus_tag="PLW22_09540"
     CDS             complement(3554..3946)
                     /locus_tag="PLW22_09540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU74984.1"
                     /translation="MDVFKLEFEVRDYECDLQGIVNNARYLNYLEHTRHKYLLSKGID
                     FARLHNEGIDLVVSRIEIDYKYSLTSGDIFVVRLTTHKEGHLRMIFDQEVIKMPEGKL
                     AVKAKVIGVGLKNGRPIKIDSIPGFDNL"
     gene            complement(3959..5221)
                     /locus_tag="PLW22_09545"
     CDS             complement(3959..5221)
                     /locus_tag="PLW22_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOU74985.1"
                     /translation="MNSQKSKGNFLPMLIIGALFFIFGFVTWLNGILIPYFKISCELS
                     DFEATLVAFAFYISYTVMALPASWVLKKTGFHRGMTVGLLVMAIGTLVFVPAALSRAY
                     WMFLSGLFIMGAGLAVLQTAANPYITIIGPRESAARRISIMGICNKLAGAFAPLILAY
                     YILNDGDAFVKSLEGMDSIARAAALDSLAHRVIGPYLVMTSILVFLGIAVRYAPLPDV
                     ETEDDSVDSVTDFTNKSSIFQFPQLILGAIALFFYVGVEVIAGDTIIRYGISQGFKIE
                     TAKVFTTYTLIAMIVGYLAGILLIPKLISQRTALIASALLGIALSIVVVLASPTYSIL
                     ALALLGLANALVWPAIWPLAIHDLGKFIKTGSAVLIMAIAGGAILPLLWGRLSDSISP
                     QMAYIILIPSYFIILYYSVSGYKLRNWK"
     gene            complement(5304..6566)
                     /locus_tag="PLW22_09550"
     CDS             complement(5304..6566)
                     /locus_tag="PLW22_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOU74986.1"
                     /translation="MYALADCNNFYASCERVFDPSLIGRPVVVLSNNDGCVIARSNEA
                     KALGIKMGEPAFKIKELIEKANVAVFSSNYTLYGDMSQRVMNTLASFTPDIEIYSIDE
                     AFLGLHGFSYVDLMDYARHIRKTTMRNTGIPISLGLAPTKTLAKVANHIAKKQPQHQG
                     VYMIANEQQRVDALKGFEIGEVWGIGRQYSKFLARYGVNTAYDFTRMPAGWVRQHMSV
                     VGLRTQKELLGIPCIDLEHTAPPKKAIATTRSFGEMQTDLGYLSEAVASFAASCAQKL
                     RKQQSVAQVVMVFAHTNYFRDDLPQYSASKTITLPVPTSSTMELIHYALIGLKSIYKP
                     GFRYKKAGVIVTGISTNQHIQTSLFDSIDREKHSKLMVVVDKLNDKYGRHTVKVAAQG
                     AGRKWKLRQERISPMYTTRWDDIITVKV"
     gene            complement(6569..7009)
                     /gene="umuD"
                     /locus_tag="PLW22_09555"
     CDS             complement(6569..7009)
                     /gene="umuD"
                     /locus_tag="PLW22_09555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOU74987.1"
                     /translation="MRLIKATPKIDFFTADTTSDLSLPLAEGIKAGFPSPAQDYIDLA
                     FDLNKELVKNPSSTFYGRVRGDSMVDEGINDGDILVIDKSLPPTDGRKAVCYIDGEFT
                     LKTIRIKKDGIYLMPANPAYKPIKVTEENDFVVWGIVTYVIHKF"
     gene            complement(7006..7368)
                     /locus_tag="PLW22_09560"
     CDS             complement(7006..7368)
                     /locus_tag="PLW22_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74988.1"
                     /translation="MDSSSTIKILVDNEKGLSADERTKLIEGTDSIYIDSRLDYHKRL
                     ARRQTVSFVLLILFALFAFGVVMFPSADPFTAGVLKGLVAGYLAALLVLVPKTTKNHS
                     RIAFVISVVKQINSPKQA"
     gene            complement(7474..8289)
                     /locus_tag="PLW22_09565"
     CDS             complement(7474..8289)
                     /locus_tag="PLW22_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74989.1"
                     /translation="MKPFKKIASYILVISLLASCATPNPVIRLKSKETNTTWESGKEF
                     TSYFKDGFVVHCAYHGTTDAYLVFDIEVINSTDEDFLVCPEKMVMFTDSGKWDPVINQ
                     IVYSTFPIYAADPELEILKNELAQSQIEANQKNLATASIAVAALALPVMVATASADAK
                     DTKQRAVNRAEVAAAAAGTTLNFAEFSQEMNNIRSDLIYASTDAWINNSLRKTTLSKN
                     EAVRGLVYFKKPDLKMYRDLRIDVPIGENNFISFNYKVNLYYPSNPNVNPAYR"
BASE COUNT         2601 a         2022 c         1893 g         2489 t
ORIGIN      
        1 taaaagtgaa taagtacaaa accatagccc tagtagcaca cgataaccgc aaacgggacc
       61 ttgtggaatg ggttgagtgg aactgggcaa aattactaaa gcacaagctc atttgcacag
      121 gcacaaccgg ccgtcttgtt gaggaggccc taaccaagat gaataacgat gccgggaatc
      181 atttgcagct tactcgcctt aaatcgggtc cacttggtgg cgaccagcag ctaggcgctt
      241 taatagctga agggaagatt gacatcctaa tctttttctg ggatcccatg caggctcagc
      301 ctcacgatgt ggatgttaag gctttgcttc gcattgctac ggtttacaat attcccacag
      361 ccaccaaccg ttcaacagcc gacttcctaa taagttcgcc acttattgaa caggagtaca
      421 ccccaaaact aaaggattac agccagtaca taaacagaga cgtttactaa aaatattgag
      481 ccatggaacg taacgagtac cgcgaaaatg taaaaaaagc cattgacgaa attttcgacc
      541 agattgaagc attgagggct aaagccgata atgcctccga taaggtaagg caggagtaca
      601 atgaaaaaat caaggagctt gaaatgcttc gtaatcaaat ggaaagcaag ctcgatgaac
      661 ttgcccaggc agccgatagc aagtgggacg aggtgaaaga tactgtaaat tccagccttg
      721 aatcgttcaa ggagggcttc aagaagttag gttcgctttt cgattagcca atacttcact
      781 tacaaatgaa tgcatttgaa ctttccctaa ttgtagggat tgtactacta ctaattgctt
      841 ggatttttgt tcttaccgat atccttcgta acagattccc tgaacggaat atgtggatta
      901 tttacttaat cattacccct cccctggctg tgttagtata tcctatagtt cgggaaagac
      961 ttttaagaaa tgcaagaaag tagtgtgagt tgaaattact cattttcaag cttaaacctt
     1021 atccgtacag ttccatcgga gtaatcggcc gagataattt tgaatctccc tatttccttg
     1081 gtgttagcca cgtgcggacc aatacaggcg caagcatcgt agtttcctac cctaataacc
     1141 ctgattgatt ccggggcatc agcgggtaat ttgcttaaat caaggaaagt tgacgcattg
     1201 cttttatcgg taaactcctc ggttacattt aggttttgtg agataacttc attcacccta
     1261 ctctcaattg tttttagttc ctcatcagtc agagctcttg ggatgcgata gtcgcacttg
     1321 gacttttttt tctccacatg gctactgaac gccctaccac aattgtaaag ccgcaccatt
     1381 gtttggttga gaatatgttc ggcggtatgc atacggggat cgtagttctt ttcgctcatt
     1441 tcagtttaaa ttttcatgta aaaatagtaa aaagggaaag caatattagc agaaacaaat
     1501 aggctgtaaa tgttgacagg aaaaagcacc catcaggcca aaaacaatta cttttgccgg
     1561 ataaaacttg tcaaatgttc gatttatcca aaaaacgctg gcagtgggct gccatgcttt
     1621 tcatggcttt tgtttgggga gcctcgttta tcctgatgaa gcgcggtctc gatgcgctaa
     1681 gctacgttca ggtggctgcg ctccgaatat ttttcagctt tttgatactt ctaccccttt
     1741 caataaaaaa cctaaagctt ataaaccgcc agaatttttg gccgctacta ggatgcggat
     1801 tactgggtaa ctttttccca gcattccttt ttacactttc tgaaacccag atctccagct
     1861 cgcttgcagg attcctcaac gggcttaccc ctttctttac ggtaattgcg ggtttgttgt
     1921 tattcaaaca taagcccaat gctttccaaa gtataggcgt tctaatcgga tttgttgggg
     1981 catcaattct aataaccaac ggaaattttt cgtcgtttgg ccatgtaaac ctttatgcac
     2041 tgctagttgt gctggccact atgatgtatg ggattaattc caatattatt cggtttaaac
     2101 tatcggggtt aaccggagtg caaattacat cgctaatctt tttcatgatt ggcattccgg
     2161 ctggtattat acttatatca acagatattg gggctgccat gcagcaccca gcatttgcaa
     2221 cgagcattct ggctaccatt gtgctagcaa catttggctc agtaattact ctatttgtgt
     2281 ataataatct tattcaccac accagcgcaa tttttgcttc atcggtaacc tacattattc
     2341 ctttttttgc gttactatgg ggcattcttg atggcgacca tattggagca ctacaaattg
     2401 tctcgttggt aataatcctg attggggttt acctctcaag cttacgccgc aagccaaaat
     2461 tgctttaaaa tcaggtcatt ttaataaaat taaatcaatt ttatccaaaa agggttgact
     2521 ttacccttat ttgtccatac ctttatttta aagtcaaaaa ccttgtttga catgacaaag
     2581 gacaaaaagg ataaaaaaaa gaaacaggcc gataaaccaa aagaaacaaa aaaaaccaaa
     2641 aagcatcacc tgcctgattt aggaaacgac cattacgaaa cggtagaggc ccatgttgat
     2701 aggatggata aaaagaccta tgagcatgaa attgaaaaac tccatgtgga actggtaaaa
     2761 ctccttgaat ggataaagca ccaaaaattg aaggttgttt tactgttcga ggggcgcgac
     2821 gctgccggaa agggcggtgt aattaaaacc atagccgaac gactaaaccc tcgtttttgc
     2881 cgaattgttg ctctaggcgt gcctaccgaa aaagagaaaa cccaatggta tttccagcga
     2941 tatgtagccc acctacccgc cgccggcgag atggttctgt ttgaccggag ctggtataac
     3001 cgagctggcg ttgaaaaggt catgggattt tgttctgagg aggagtacac tgagtttcta
     3061 cgctcatgcc ctgagtttga acgtatgctg gttcgttcgg gtataatact cattaaatac
     3121 tggttctccg ttagcgacaa ggaacaggaa cgtcgctttc aggagcgtat cgacgatcca
     3181 accaagcgat ggaaactcag ccccatggat attgaatcgc gtaaccgctg ggtggaatac
     3241 tccaaggcaa aggatgttat gttcgcccat accgatataa agcaagctcc ctggtgggta
     3301 gttcgagccg acgataaaag gcgtgcccgc cttaactgta tccatcacct gctaagcctc
     3361 ataccttacg aagatcttac cccaccaccg gttaagcttg aaccccgacg gattgacgag
     3421 agctatgttc gtccacctat caccgaccag aactttgttc ccgaaattta ttaattgcaa
     3481 atagggctgc tcgcatgggc agcccaaaat gtttaaaatc gccaataaaa aaaaatcaat
     3541 tgataaatcg cggttaaagg ttgtcaaagc ccggtatact atcaatcttg attggacggc
     3601 cattttttag cccaacgcca attaccttag ccttaacggc taatttcccc tcgggcattt
     3661 taatcacttc ctgatcaaag atcattcgta aatggccctc cttgtgggtg gttaaccgaa
     3721 caacaaatat atcgccactg gttagcgagt acttgtagtc aatttcaata cgtgacacca
     3781 caaggtcaat gccctcattg tgcaacctgg caaaatcgat tcccttgctc aacaagtact
     3841 tgtgacgggt atgttcaagg tagttcaggt agcgagcatt gttaacaata ccctgcaagt
     3901 cgcattcgta atcacgaacc tcaaactcta gcttaaaaac atccataatt tttacgtttt
     3961 acttccagtt ccttaactta tatccgctca cagagtagta aagaattata aagtacgatg
     4021 gtatcagaat aatgtatgcc atttggggcg aaattgaatc ggatagtcgg ccccaaagca
     4081 agggcagaat ggcaccaccc gcaatggcca tgataagcac cgccgaaccc gttttaataa
     4141 atttgcctaa atcgtgaatg gccaatggcc agatggcagg ccaaaccaat gcatttgcta
     4201 aaccaagcag tgcaagtgca agaattgagt aagttggcga tgccaaaact acgacaatac
     4261 ttagagcgat acccagcaaa gcagatgcaa taagagccgt gcgctgcgaa ataagttttg
     4321 gtatcagtaa tattccagcc aggtatccaa ctatcatggc aataagggta tatgttgtaa
     4381 aaacctttgc ggtttcaatt ttaaaacctt gcgatattcc atagcgaatt atggtatcgc
     4441 ctgctattac ctcaacacca acatagaaaa acagggcaat agcaccaagt atcagctgcg
     4501 ggaactggaa aatacttgat ttattagtga aatcagtaac agaatctacg gaatcatcct
     4561 ctgtttccac gtcgggcagc ggggcataac gtacggcaat ccccaggaaa accagtattg
     4621 atgtcattac caggtacggc ccaatcactc tgtgcgctag gctatcgaga gccgcagcgc
     4681 gggcaatact gtccatcccc tcaagtgatt tcacaaaggc atcgccatcg ttaaggatgt
     4741 agtaggccag tataagcgga gcaaaggctc cagctaactt attacaaatc cccattatac
     4801 ttatacgcct tgcagcactc tcgcggggtc ctattatggt gatatacgga tttgcagctg
     4861 tttgcagcac agccaaccct gctcccataa taaacaggcc actcagaaac atccagtaag
     4921 ctctgctcaa cgctgcggga acaaacacaa gggttcctat agccatcacc agtaaaccta
     4981 ctgtcatgcc cctgtgaaaa cctgtctttt tcaacaccca cgatgccggc aaagccatta
     5041 cggtatagga gatataaaag gcaaaagcta ccagggtagc ttcaaaatcg ctaagctcac
     5101 acgaaatttt aaaatacgga ataaggatgc cgtttaacca ggttacaaaa ccaaaaatga
     5161 agaataacgc tccaataatg agcataggca aaaaattgcc tttacttttt tgagagttca
     5221 ttgcagttgt tttaggttaa ggtaccccaa aaatagaaaa atattgatga gcgaagaggc
     5281 caaatcaaaa taaaaaagga aacttaaacc tttacagtaa taatatcgtc ccagcgagtg
     5341 gtgtacatgg gcgaaatacg ttcctggcgt aatttccatt tacgtccagc cccctgagcg
     5401 gctactttaa ccgtgtgcct gccatactta tcgtttagtt tatccacaac caccattagt
     5461 ttactgtgtt tttccctgtc aatgctatcg aagagcgagg tttgaatatg ctggttggtt
     5521 gaaatacccg ttacaattac tcccgccttc ttgtaccgga atcccggctt gtatatactt
     5581 tttaatccaa taagggcgta gtgtatcagc tccatggtgc tgctcgtggg cacaggcagg
     5641 gttatggttt tacttgccga gtactggggt aaatcgtcgc gaaaatagtt ggtgtgtgca
     5701 aaaaccatta ccacttgcgc aaccgactgc tgcttacgca atttctgggc gcaactcgct
     5761 gcaaacgatg ccaccgcctc actcaggtat ccaaggtcag tttgcatctc accaaaggaa
     5821 cgggttgttg caattgcctt tttgggtgga gcggtatgtt ccaaatcaat acagggtatt
     5881 cccagtaact ccttttgggt tcggagtcca accactgaca tgtgctgcct tacccaacct
     5941 gcgggcattc gggtaaaatc gtatgctgta ttaacaccat agcgggccag aaacttactg
     6001 tactgccggc ctattcccca aacctcacca atctcgaacc cttttagcgc gtcaacacgc
     6061 tgctgctcgt tggcaatcat ataaactccc tggtgttgtg gctgcttttt tgcgatatgg
     6121 ttagcaacct ttgctaacgt tttggtaggg gctaagccca agcttatggg aataccggta
     6181 ttccgcatgg tggttttccg aatatgtcgg gcataatcca ttaaatcaac atagctgaaa
     6241 ccgtgcagcc caagaaaagc ctcatcaatg gaatatattt cgatatcggg ggtaaagctg
     6301 gcaagggtat tcatcaccct ttggctcata tcgccataaa gcgtatagtt tgatgagaag
     6361 acagcaacat tggctttttc aatcagttcc ttaatcttga aggctggctc acccattttg
     6421 ataccaagag cttttgcctc gttgctgcgg gcaattacac aaccatcgtt gttcgacaga
     6481 acaacaacag gcctcccaat gagcgaaggg tcaaaaacac gctcgcacga agcgtaaaag
     6541 ttattgcaat cggcaagagc gtacatggct aaaacttatg gatgacgtag gttacaatac
     6601 cccataccac aaagtcgttt tcctcagtaa cctttatggg tttatatgcc ggatttgccg
     6661 gcataaggta gattccatcc tttttaatcc gaatggtttt aagcgtaaac tcaccatcga
     6721 tatagcaaac tgctttacgc ccatcggtgg gtggtaacga tttgtcgata accagaatat
     6781 cgccatcgtt aatcccctca tcaaccattg aatcgccacg gacacgacca taaaaagttg
     6841 aacttggatt cttgacaagt tccttgttta aatcaaaggc taaatcgata tagtcctgcg
     6901 ctggcgacgg aaaaccagcc tttattcctt cggccaaagg aagtgaaagg tcggatgttg
     6961 tgtcggcagt aaagaaatct atcttaggtg tagcttttat cagcctcatg cttgtttagg
     7021 ggagttaatc tgtttaacta cactaataac aaatgcaatt cgcgaatggt ttttagttgt
     7081 tttaggaaca agaacaagga gtgcagcaag ataccctgca accaatcctt ttagtactcc
     7141 tgcagtaaaa ggatcggcgc taggaaacat taccacgcca aaggcaaaaa gtgcaaaaag
     7201 tatcagcaaa acaaagctta cagtttgcct tcgggctaac cgcttgtggt aatcgagcct
     7261 tgaatcaatg taaatactat cagtgccctc aattagtttt gtacgttcat cggccgacaa
     7321 tcccttttcg ttatcaacca atatcttaat tgttgaactg ctgtccataa cattcagatt
     7381 ttttacaaat ttactacatt aaaccatgaa cttatcagga atactaaaaa gaaaaaaggc
     7441 cctcaggcct tttcccttga ttgttaagca aatctaccta tatgcgggat taacattcgg
     7501 atttgagggg tagtacaggt ttaccttgta gttaaaggat atgaaattat tctcgccaat
     7561 gggaacatca atgcgtaaat ccctgtacat tttcagatcg ggctttttaa agtaaactag
     7621 cccacggact gcctcgtttt tagatagggt tgttttgcgg agtgagttat tgatccatgc
     7681 atctgttgat gcgtaaatca gatcgcttcg gatattattc atctcctggg agaattcggc
     7741 aaagttaagg gttgtccctg ccgctgctgc agccacctcg gctcggttaa ctgcacgctg
     7801 tttggtatcc ttggcatcgg ctgaggctgt tgcaaccatt accggtaatg ccaatgcagc
     7861 tactgcaatt gaagcagtag caaggttttt ctgattagcc tcaatctgcg attgcgccaa
     7921 ttcattttta agaatttcta gttcgggatc agcggcatag attgggaatg tgctgtacac
     7981 aatctggttg attaccggat cccacttccc actatcggta aacatcacca ttttttcggg
     8041 gcaaaccaga aaatcctcat cggtagagtt aatcacctct atatcgaaca ccaagtaggc
     8101 atctgtagta ccatggtaag cgcaatgaac cacaaagcca tccttaaagt aactggtaaa
     8161 ttctttccca ctttcccagg ttgtatttgt ttccttagat tttaacctga taactgggtt
     8221 tggagttgca caagaagcca ataagcttat tacaaggatg taactggcta tctttttaaa
     8281 aggtttcata gattttaggt agttggttta acgtaagccg tcaaataaca tgccatgaac
     8341 ctaaaaaaat caagcattct catacattaa tttgatgttg aaaaacacct atggaacaat
     8401 ttaaatgaaa gatcagccca gttgggttaa aaacgagatt cctcgctgtg ttcgggatga
     8461 cggtttacga tattcgtgaa tcgctgaaca ttgttcgtaa cacctttgtc atgttgagcg
     8521 gtagcgaaac atctctttag ggccgattag agttccttcg ctccccgcta gggcgggaca
     8581 ggccgctcag gatgacaagc ggagttgaca gttgatagtt gatagtgaaa aaaagcattt
     8641 cacgaaacat ctaactccaa acacccctat acactgtaca ctgtacactt ttcaatagat
     8701 cattcgctac gctcaggatg acaaatgaag ttgttcgtgt aagttgttcg ttgctcgtga
     8761 aactattgtc atgctgagct agccgaagca tctcctttcc accttttatg agatccctcg
     8821 ctttgctcgg gatgacaaaa accctatctt ccttttctgc tcccttgtca tgctgagcta
     8881 ggcgaagcat ctctttacct tcaactcttc ttttttaatg cgattcctta accaacacgg
     8941 agtgccatac gcagaggcat aaaagcctga cccggcaatg ggtcgggggt ggaacgtggc
     9001 ggtgg
//
LOCUS       DAOOWJ010000104         8984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_7726_length_8984_cov_7.560197, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000104 DAOOWJ010000000
VERSION     DAOOWJ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7726_length_8984_cov_7.560197"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1799
                     /locus_tag="PLW22_09570"
     CDS             129..1799
                     /locus_tag="PLW22_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74990.1"
                     /translation="MKTSRFLKSLFIVGLAGLGGCYDFSQFDNIKVEPISPKMVVPVV
                     KSTISFNDIVEREDANTIIFTKPGDTRFFIAFRDTMDLFNAADRYSFPDFNFTKQYQL
                     DAGEVPGVPVPAGQTWGPFTKQYTETYNSIAGAEVKLVKLSAGNINMVITNNFQNSIS
                     AIIRFPSIRDQLGNPLVFAMNTTLPSQTYTNNQDLTDYTLNLTSGLLYNTLTVEVELT
                     IHSLGNPITLSDDANIQVSLTGLDFDYLQGKLNETFPLNDINLTLDAFRTSIDADFSL
                     SEPKLTMTFENLFGIPLAFSTVNFDASNTNTSKQVSLVNEGVSPTGSLLLTQPNNIKY
                     LTSLTQQQPEVDLKYVDRNNSNLEDFLAIAPNEILISPTVTVGDPTTNHDYFVRKTST
                     LRMINEIEFPLAGWVDNLEICDTLEVELPDLEKDLKLVNDNALKIRLKFKFINSIPFN
                     IYIQGYFLDDANTLLTQLYDEASELMLVKSSAINPTTGKTSTPTTHWAYIDISKSKYD
                     QMKNATNLVIQARMQTGGNTHQNVVVESTNTLETMFSVELEGNVDLNN"
     gene            1822..3168
                     /locus_tag="PLW22_09575"
     CDS             1822..3168
                     /locus_tag="PLW22_09575"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOU74991.1"
                     /translation="MKRYIFSTFILFTSLLAKAQPEVTLPYMRNVFQASFVNPTVMPE
                     HTFSMGFSVFGQAISNGFQPKSFMNYRSDTMYVNLNSLLGDMNDKNLIYVGENVDIFH
                     IRVKGKSSFTWFAMRQNATVTLQYPRELFSLAIEGNKQFVGSTLDLSNLKSDVSVYNE
                     YTIGYLKDLPQWTFAGRVSLLQGLANISFNPKVMQVEIDTAMYAHTATADGQLRTSGT
                     PKNSEGDISFDNVDDQWVTNRLTGFGNMGFAISGGATYKFDERMKLSFGFSDIGFIKW
                     KDEIQTYTLKGTTKFEGLDILGDFLRGNDVDVDSLLQKMEDDFTRDTVAVSYTTWLNP
                     KFNFSATYDLTRRTTLGLTFATFYNKRLYPSITLGVSQGIGRFFQLVGTASIYQRSIP
                     NLGVGIVIKPGPLQLFVVADNLYLAIDPLAFTNANVRVGINFVFGRVNPPQGLPYR"
     gene            3219..>5311
                     /locus_tag="PLW22_09580"
     CDS             3219..>5311
                     /locus_tag="PLW22_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU74992.1"
                     /translation="MKLKVVSRILITLSIISFGSKVFAQTGFTPQKLSGEINTGYAEI
                     NPILSPDGKTLYFTRVNHPENRFGEVDSQDIWCTTLNPDGTWSEAKRLPNTINLGRYN
                     AIFGILDDGKTFVINGHYTESGKYWTERGLSVIERIDENTWGTPRPLNVKGFTRMNKG
                     LTTTAYLTPDGKYLLTSFSKRAGGKNHSIYLSVKEGDGYSKPQKVKIGDGNLGESYEA
                     PFLSKDGNALFFSCKVDGNNNIYMANRTDDTYLNWSTPTALNDTINTPGWENYYRLNA
                     KESWAYYCSSSSKKEHSEIMRVKIYEEFPYVKLTGLVMNKAEESLMLADTGYNILVNG
                     KKSDSLKIDRASASYELLIPFGKKHSLLPELANWVGIPDTLDLTAVKEYSEMSKNLYL
                     QPIPLVRIYGKIINTRTGLPINPEMKYQILVNGVTSDSVKYEAEIAKYGMTLPLGNRY
                     ILSLNLPNFTSKTDTIDVTNAKYYTEKQVDFYATTVPWVEVNGVALDNSTFTPIIGAS
                     APKLKINGSTADSVSIDPVTGEFKVRLPFGKKYVTAIESKDYNPLDNQLDLTGYVEFT
                     QVKHEVYAERKDANMAILSGKVINLKTGQPLEKEVPVKLKVNGTETRGFIYDSTSASY
                     TLKLPVGVSYDVLPSVKNFYNKYEQVDLTKVKKGTKIAKNFYVTPIEVGQSVNIDFIY
                     FDVGKATLKPQSFRSL"
     assembly_gap    5312..5411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5412..5990
                     /locus_tag="PLW22_09585"
     CDS             <5412..5990
                     /locus_tag="PLW22_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOU74993.1"
                     /translation="MVALLALVNPIQKVFVMTTLQAQFDEAKTRFIATKATLTAFIVL
                     IFFLLLGELVFSYVFHVELYAFQITSGIVLFYNGINGLQKGTFIRVDSGVNLKDITAV
                     PIAIPMIAGPATITAAVTFPAHYGHTNTIVSMAVALGVNFLFMYYSRPIGKALNRFNL
                     MGPLVRIFGLIVATIGVQMMLSGIASFWISIR"
     gene            6064..6894
                     /locus_tag="PLW22_09590"
     CDS             6064..6894
                     /locus_tag="PLW22_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU74994.1"
                     /translation="MQQNLIDIDNVIASKSPRLQRALPRFIIRYLKRIIHQDEINEVL
                     LNHGDKQGVDFIDEVLKVMQVTYSVKGIENLKPDGRYLFASNHPLGGLDGMILIHLLG
                     KRYPEVKFPVNDLLMHINQLHNIFIPINKHGAQGSQNVKLMDATFASDAQVLYFPAGL
                     CSRKQHGRIEDLEWKKSFIVKAIQHKRDVVPVFFSGHNSNFFYNLAKLRTFLGIKANI
                     EMLYLVDEMFKQKGKRIAVIIGEPIPCTTFDSSKSPAEWAKWVKSVVYELGKMNFNKF
                     "
     gene            6900..7868
                     /locus_tag="PLW22_09595"
     CDS             6900..7868
                     /locus_tag="PLW22_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU74995.1"
                     /translation="MKQIIEPVDRELLLAELTPDKLVRHTNNANNELYIFTAHNAPNL
                     MREVGRLRELTFRSAGGGTGEEVDIDEYDTCENCYKQLIVWDPHEKEILGGYRFHSVD
                     PSKGEEINLSTKHLFHFSNKFIQEYMPQMIELGRSFVQPKYQSLSLSGKGLYALDNLW
                     DGLGALIVLNPHIRYFFGKVTMYRTYNVEARNILLYFFEKYFRDAENLVTPIHPIEVN
                     IDRQAMAQVFTGKNYEEDYKILSKKIREFGERIPPLINSYMNLSPSMKVFGTVINPYF
                     GEVEETAILITIPDIYQHKVERHISTFIKKLKDKYHLGLRWGKDSK"
     gene            complement(7942..>8984)
                     /locus_tag="PLW22_09600"
     CDS             complement(7942..>8984)
                     /locus_tag="PLW22_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOU74996.1"
                     /translation="DVKEIKPGYALIIKRNGTILHEEIRIPQARKSCSFERIYFSRGT
                     DKDIYKERKRLGEFLTPAILKAINYDHENTVFSYIPNTAETAFLGMVKGIEDYIKIEK
                     KRRILEKGANLTEKDIDEIISTRLRVEKIAVKDVKLRTFIAQDKGREDLVGHVYDITY
                     GTLRPGVDNLVVIDDSIVRGTTLRESILRILDRLNPARIIVVSSSPQIRYPDCYGIDM
                     AKLSDFVAFRAAIELLKETGQEGVINSVYRKCKEQQNLPKEQIINYVKEIYRPFTDEQ
                     ISQKIAQLLTPKDVKAEVKLVYQSIENLHKSCPNDQGDWYFTGDYPTPGGNRVVNNSF
                     INFIEGRNQRAY"
BASE COUNT         2643 a         1809 c         1853 g         2579 t
ORIGIN      
        1 aattaattca aagtctaaca gtattatgga ttagttaaga atagtttgac tcgtattagt
       61 attattcgta acttgagcag tcttttaaat aggaattatt aacattgatt gaagtaaaaa
      121 aggggaatat gaaaactagt agattcttaa aatcactgtt tattgttggt ttggctggac
      181 tgggagggtg ttacgatttc tcgcagttcg ataacatcaa ggttgaaccc atttcgccta
      241 aaatggttgt cccggtggtt aagtcaacca tttcgttcaa cgatattgtt gagcgagagg
      301 atgctaatac aataatattt accaaacctg gcgatacccg gttttttata gcattcaggg
      361 atactatgga tttgtttaac gcagccgata ggtattcgtt tccggatttc aactttacca
      421 agcagtatca gctcgatgca ggtgaagtgc caggtgttcc tgtgcccgca ggacaaacat
      481 ggggaccatt tacaaagcaa tacactgaaa catacaatag cattgctgga gctgaagtaa
      541 agttagtaaa gctatcggct gggaatataa acatggtaat aaccaataat ttccagaaca
      601 gcattagcgc aattatccgg ttcccttcta tacgcgacca attagggaac cctctagttt
      661 ttgccatgaa tactactctt cccagccaga cttacaccaa taaccaagac ctaacggatt
      721 acactctaaa ccttactagt ggtctcttat ataacaccct tacagtggag gttgaattaa
      781 ccatacattc gttgggtaat cctataactc tatcagatga tgctaacatc caagtttccc
      841 ttacaggatt agattttgat tacttacagg gtaagctaaa tgagacattt cccttgaacg
      901 acatcaacct tactctcgat gcttttagaa catcgattga tgccgacttt tccctttcgg
      961 aaccaaagct taccatgact tttgagaatt tatttggcat accacttgca ttcagcactg
     1021 ttaactttga tgcctcaaat actaacacca gtaaacaggt tagccttgtg aatgagggag
     1081 tatcaccaac cgggtcgttg ctccttactc agcccaacaa cattaagtat ttaacctcgt
     1141 taacacaaca gcaacccgag gttgacttaa aatacgttga tcgtaacaat tcaaaccttg
     1201 aggattttct ggccatagca cccaatgaaa tcctaatcag cccaacggta accgttggtg
     1261 atcccactac caatcacgat tactttgttc gcaaaacttc aacactaaga atgataaacg
     1321 agattgaatt tccccttgcc ggctgggttg ataatttaga aatttgtgat accctagagg
     1381 ttgaactacc cgatttggaa aaggatttaa aacttgtaaa cgataacgct cttaaaataa
     1441 ggttgaagtt taagtttatc aatagcattc catttaatat ttatattcaa ggatactttc
     1501 tggacgatgc taacacatta cttacccagc tttacgacga ggcttcggag ctgatgctgg
     1561 ttaaaagctc tgcaataaat ccaacgacag gtaaaacatc aacccccaca actcattggg
     1621 cttacatcga tataagcaag agcaagtacg atcaaatgaa aaacgcaacc aatctggtta
     1681 ttcaagcccg aatgcaaacc ggaggtaaca cccaccaaaa tgtagttgtg gaatcaacca
     1741 acacccttga aacaatgttt agcgttgagt tagagggtaa tgtggattta aataattagt
     1801 attcagaaat atatgactcc aatgaaacga tatatttttt ccacattcat tttatttacc
     1861 tcactgctgg caaaagccca gcccgaggta accctgccat acatgcgtaa tgtgtttcag
     1921 gcatcgtttg taaaccctac cgtaatgccc gagcatacct ttagcatggg atttagcgta
     1981 tttggtcaag ccataagcaa tggttttcag ccaaagagtt tcatgaacta tcggagcgat
     2041 acgatgtatg ttaaccttaa cagccttttg ggcgatatga acgataaaaa tttaatctat
     2101 gttggggaaa acgtcgatat ctttcatata cgtgtgaaag ggaaatcgtc ctttacctgg
     2161 tttgccatgc gtcagaacgc aacagtaaca ctccagtatc cacgtgaact ttttagctta
     2221 gctattgagg ggaataagca gtttgttggc tcaaccttgg atttatccaa cctaaaatcg
     2281 gatgttagcg tttacaacga gtacaccatt ggatacctta aagatttacc ccaatggact
     2341 tttgcaggaa gggtaagttt gctgcaaggt ttagccaata tcagttttaa tcccaaagta
     2401 atgcaggttg aaattgatac tgccatgtat gctcatactg ctactgccga cggtcaactc
     2461 cgtacctcgg gcacacctaa aaacagtgaa ggcgatatca gttttgataa tgttgatgac
     2521 caatgggtta ccaataggct tactggtttt gggaacatgg ggtttgccat tagtggaggc
     2581 gcaacataca agttcgatga aaggatgaaa ctatcctttg ggttttccga tataggattt
     2641 ataaaatgga aggatgaaat tcaaacatat acccttaaag gaaccaccaa atttgaaggt
     2701 ttagatattc ttggcgattt cctgagagga aacgatgttg acgttgactc gttactacag
     2761 aaaatggaag acgattttac ccgcgatacc gtagcggttt cctacaccac ttggttaaat
     2821 cctaagttta acttttcggc cacttacgat ttaacccgaa ggacaaccct tggccttacc
     2881 tttgcaacat tttataataa acgattatac ccttcaataa cgcttggtgt ttctcagggc
     2941 attggccgct tcttccagct agtaggaaca gcttcaattt accaaagatc catacccaac
     3001 ctgggggtag gaattgtaat taaaccaggg cctttgcagc tatttgttgt agccgataat
     3061 ctgtacctgg caattgaccc gctggcattc accaacgcca atgtgagggt aggaatcaat
     3121 tttgtttttg gcagggttaa tccacctcaa gggttgcctt accgctaatt ttccaaatac
     3181 cgattattca gcaattattc aatttaaagg taaaagcgat gaaactaaaa gttgtaagca
     3241 gaatcctcat aacactcagc ataattagtt ttggttccaa ggtgtttgca caaaccggtt
     3301 ttactccaca aaagctaagt ggcgaaatta atacagggta tgctgaaatc aatccaatac
     3361 taagtcccga tgggaaaacc ctttatttca caagggtaaa tcaccccgag aataggtttg
     3421 gcgaagtcga tagtcaagat atttggtgca caacattgaa ccccgatggc acatggagcg
     3481 aagctaaacg cttacccaat accattaacc ttggtcgtta caatgcaatt tttggcatac
     3541 tcgatgatgg taaaacgttt gtaatcaatg gccattacac tgagagtgga aagtactgga
     3601 cagaaagagg cctatcagta attgagagga ttgatgaaaa cacatggggt acgcccaggc
     3661 cgttgaatgt gaagggattt actaggatga acaagggttt aacaacaact gcatacctta
     3721 cccccgatgg gaaatattta cttacgtcgt tcagtaagcg tgccggtggg aaaaatcatt
     3781 caatctacct ttcggtaaag gagggcgatg gatactctaa gccccaaaag gttaaaattg
     3841 gtgatgggaa cctcggcgaa agctatgagg cgccattcct aagcaaggat ggcaatgcgc
     3901 ttttcttcag ctgtaaggtt gatgggaaca acaatatata catggctaac cgaacggacg
     3961 atacttacct aaactggtcg acgcccactg ccctgaacga taccattaat acaccgggtt
     4021 gggaaaacta ctaccgttta aacgctaagg aaagttgggc ctactactgc tcatcaagtt
     4081 caaagaagga gcattcggaa attatgcggg taaagattta cgaagagttc ccctacgtaa
     4141 agttaaccgg gcttgtaatg aacaaggccg aggagagtct tatgcttgcc gataccggat
     4201 acaacattct tgttaatggg aaaaagtcgg atagcttaaa aattgatagg gcctcagctt
     4261 cgtatgagct acttattccc tttggaaaaa aacattcctt acttcctgag ctggctaact
     4321 gggtgggcat ccccgataca ttggatttaa ctgccgttaa ggaatactct gaaatgtcaa
     4381 agaatttata cctgcagccc ataccattag taaggattta cggtaagata ataaatactc
     4441 gaactggact ccctattaac cctgaaatga aataccagat tttggtaaat ggggttacaa
     4501 gcgattcggt aaagtatgag gccgaaatag ccaaatacgg tatgacccta ccattaggaa
     4561 acaggtatat actctccctt aacttgccca actttacaag taaaaccgat actattgatg
     4621 taaccaatgc aaagtactat acggagaaac aggttgattt ttatgccaca acagtaccct
     4681 gggttgaggt gaatggggtt gcgctcgata attcaacctt tacgccaata attggggctt
     4741 cagcaccaaa acttaagatt aatggttcaa ctgccgatag cgttagcatc gatccagtaa
     4801 ccggtgagtt taaggtacga ttgccttttg gaaagaagta cgttacagca attgagtcaa
     4861 aggattacaa tccgttagat aatcagctcg atttaaccgg ttacgttgag tttacccagg
     4921 ttaagcatga ggtttacgct gagcgtaaag atgctaacat ggcaatacta agcggtaaag
     4981 ttattaactt gaagacagga caaccccttg aaaaagaggt gcccgtgaag ctgaaggtaa
     5041 acggaactga aaccagagga tttatttacg actccacatc ggcaagctat accttgaagt
     5101 tgccagttgg tgtgtcatac gacgttctgc cttccgttaa aaacttttac aataagtacg
     5161 agcaggttga cctaaccaaa gtgaaaaagg gaactaaaat tgccaagaac ttttacgtaa
     5221 ctccaattga ggttggtcaa tcagttaata ttgacttcat ctactttgac gttggtaaag
     5281 caaccctgaa accacagtcg ttccgctcac tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn natggttgcc ctgctggcat tagtaaatcc aatacagaag gtatttgtta
     5461 tgaccaccct acaggctcag tttgatgagg caaaaactcg gtttattgcc actaaggcta
     5521 cacttacagc gtttattgtc ttgatattct ttctgctttt aggcgaattg gtatttagct
     5581 atgtatttca tgttgagcta tatgcttttc aaattacatc gggcatagtg cttttttaca
     5641 atggaataaa tggattgcag aagggcactt tcatacgagt tgattcaggc gtaaacctta
     5701 aggatataac ggcggttcca atagccattc cgatgattgc tgggcccgcc accattactg
     5761 cagcggttac tttcccagca cactacggtc acaccaatac cattgtatca atggcggtag
     5821 ccttgggggt aaacttcctt ttcatgtatt actcaaggcc aataggaaag gcccttaacc
     5881 ggttcaacct aatgggccca ctcgtgcgta tattcgggct cattgttgct accattggtg
     5941 tacagatgat gcttagcgga atagctagct tttggatttc aatccgatag attgcagtat
     6001 caggaagaat gtagaataac catacttggg ttttattata tttgcactta aatttattac
     6061 gaagtgcaac aaaacctaat cgacatcgat aatgtaattg cctccaaaag tcctcgactt
     6121 caaagggcat taccccgttt tataattcgg taccttaaac gaatcattca tcaggatgag
     6181 attaatgagg tgcttctgaa tcatggcgat aagcagggag tagattttat tgacgaagtg
     6241 cttaaggtta tgcaggtgac ctattcggta aaaggaattg aaaatcttaa gcccgatggt
     6301 aggtacttgt ttgcctcgaa ccaccccctt ggaggtctgg acgggatgat tctcattcat
     6361 cttttgggta aacgttaccc cgaagtaaaa ttccctgtga acgatttgct aatgcatatt
     6421 aatcagctac ataacatatt catacccatt aataagcatg gtgcgcaggg ctcccaaaat
     6481 gtaaagttga tggacgctac ctttgcatcc gatgcacagg tactttactt cccggcaggc
     6541 ctatgctctc gcaagcagca tggccgaatt gaggatttgg agtggaaaaa aagctttatt
     6601 gtaaaagcaa ttcagcataa gcgcgatgtg gtgcccgttt tctttagtgg ccacaactcc
     6661 aatttctttt ataatttggc aaagctcaga acttttttag gaataaaggc aaatatagag
     6721 atgctttacc ttgttgatga gatgtttaag caaaaaggca aacgcattgc tgtaataata
     6781 ggggagccaa ttccctgcac cactttcgat agttctaaat ctccagctga atgggcaaaa
     6841 tgggttaaat cggtagttta cgagttggga aaaatgaact ttaataaatt ttaaccgaca
     6901 tgaaacaaat tattgaaccg gttgacaggg agctgctcct tgcggagctt acccccgata
     6961 aacttgttag gcataccaat aacgcaaaca acgagctcta catatttaca gcccacaatg
     7021 ccccaaacct gatgcgtgag gttggacggt taagggaact cacattcaga tcggcaggtg
     7081 gtggaaccgg tgaggaggta gatatcgatg agtacgacac ctgcgaaaat tgttacaagc
     7141 agcttattgt atgggatccc catgaaaagg agatacttgg tggctaccgc tttcatagcg
     7201 ttgatccctc taaaggcgag gagataaatc tatctaccaa acatttgttt cacttttcca
     7261 ataagtttat tcaggaatat atgcctcaaa tgattgagtt gggtcgatca tttgttcagc
     7321 ctaaatatca atcactttcc cttagcggaa agggattgta tgcgctcgat aacctttggg
     7381 atggcctggg agcactcatt gtgctaaatc ctcatatcag gtatttcttc ggcaaggtta
     7441 ccatgtatcg cacgtacaat gttgaagcac gtaatatttt gctttacttt tttgaaaagt
     7501 atttcaggga tgctgagaac ctggtaacac caattcatcc aattgaggta aatatcgata
     7561 ggcaggctat ggcccaggtt tttactggta agaattatga agaagactac aagattctgt
     7621 ccaaaaaaat tcgcgagttt ggcgagcgta ttccaccgct tattaactct tacatgaact
     7681 taagcccatc catgaaagtg tttggaactg ttattaatcc ttactttggc gaagttgaag
     7741 aaacagctat tctcataacc attcctgata tttaccagca taaggttgag cggcatatat
     7801 caacctttat aaagaagtta aaggataagt accacttggg tttaaggtgg gggaaggatt
     7861 caaaataacc ctgaaagtta aacctctact atttatcctt ttttttatcc tttaaccttt
     7921 atcctttaac ctttatcctt gtcaatacgc cctttgattt cggccctcaa taaagttgat
     7981 aaacgaattg tttaccactc ggtttccacc gggggtgggg tagtcgcctg taaagtacca
     8041 gtcgccttgg tcgttagggc aagacttgtg tagattttca atggattggt aaactagttt
     8101 tacttctgcc tttacatcct ttggggtaag aagctgagct attttttgcg aaatctgctc
     8161 atcggtaaac gggcggtaga tttcttttac atagttaatt atttgctctt tgggcaggtt
     8221 ttgttgctct ttgcacttgc ggtaaacgct attaataaca ccctcttgtc cggtttcctt
     8281 gagcagttca atggctgcac ggaaggccac aaaatcgctc agtttggcca tatcaattcc
     8341 gtagcaatcg gggtagcgaa tttgtggcga tgatgataca acaattatgc gggcagggtt
     8401 aagcctatca agtattcgga gaatgctttc ccgaagggtt gtccctcgaa caattgaatc
     8461 gtcaattacc accaggttat caactccagg gcgaagagta ccataggtga tgtcgtaaac
     8521 atgcccaacc aaatcttcgc gacctttatc ctgtgcaata aatgtgcgta gcttaacgtc
     8581 ttttacggca attttttcta cccttaggcg agtggatatt atttcatcga tatccttttc
     8641 ggtaaggtta gccccttttt ccagaattct gcgtttcttt tcaattttta tgtagtcctc
     8701 tatgcctttt accatgccaa gaaaagctgt ttcggcagta ttgggaatgt atgaaaaaac
     8761 agtattctca tggtcgtagt ttatggcttt cagtatggca ggggttaaaa attcgcctag
     8821 ccgtttacgt tccttgtata tgtccttatc ggttccccgt gagaaataaa tccgttcaaa
     8881 cgagcatgat tttcgtgctt ggggtattct tatttcctcg tgtagtattg taccattgcg
     8941 cttaattata agcgcatagc cgggcttaat ttcttttaca tcgt
//
LOCUS       DAOOWJ010000105         8974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_7739_length_8974_cov_7.363382, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000105 DAOOWJ010000000
VERSION     DAOOWJ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7739_length_8974_cov_7.363382"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PLW22_09605"
     CDS             <1..1124
                     /locus_tag="PLW22_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU74997.1"
                     /translation="RQAMYEHVVIRTRKGFYTPTYHGIDWNFMAEHYRKFLPHIGNGF
                     EFAELLSELLGELNVSHSGARFYLNNPNGDQTASLGIIPDVTYQGQGIRVDEILLGGP
                     LDRKELSLSTPFIITAIDGNTIGTDKDWAYYLNRKADRYILLTLTELSNGKERQVTVK
                     PTNLSQESRLLYRRWVKRNADEVEKLSNGTLGYVHIPGMSDGPYRNIYGEMMGKYYDR
                     SGVVVDTRFNGGGDLVSDLAMFFTGTKFITYHNHVRELGSEPTFRWTKPTVALVNEAN
                     YSDGSCFACGYKQLGIGKLIGMPVPGTCSFAGWETLQDNQIRWGMVPVSAKDMYGVWM
                     ENVEAIPDYVIMNEPSVAPKGRDQQLEKAIKALMEATVK"
     gene            1585..3582
                     /gene="gatE"
                     /locus_tag="PLW22_09610"
     CDS             1585..3582
                     /gene="gatE"
                     /locus_tag="PLW22_09610"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOU74998.1"
                     /translation="MAKKQTPGKHFDPQKNYRETMKAIGYVTRAEATPKDYERIGFKS
                     GLEVHQQLKTKQKLFCRCPAGIYHDDNDYHAEIIRHMRPTLSEMGEYDGTALMEFKTR
                     KEIVYRINNKTTCTYEIDDTPPFPINREALEIALEISLLCKLNIVGEVHITRKQYLDG
                     SIPTGFQRTAILGVEGQIQLKNKKVGLIQLSIEEDACREISDVGHRRIYKTDRLGMPL
                     IETVTHPQCLTPDELREAADYIRFLNRSTGKVRTGIGAARQDTNVSCEGGTRVEIKGV
                     SHNKWLPMLSHNECFRQWALLHIRKLLLERVPLPEKWVMRHIEIDPYSQGIEHAVANR
                     FADMGYKVVAIALPAFHSILSHFTQPGKCFTNELADRLKVIACIERPNITSNEDLEIP
                     LSESDTTRLASLLKASPDDAIVLIWGPANDIPTAIETIEERCRMAFEGVPRETRRPLA
                     DGTTIFERVLPGADRMYPDTDSAPIPLDEAYIEKLRERLPIDIWHRFQQLAQMQIPED
                     AHPFLLRRNLVPVIEEIVGLGFPSRYVGSLLGHKLKRIEGKYPKHKDFNYSRIVELFR
                     FIKENKLHHSIAKLMLPVVYQYPNMDFNSVLTSINFKRKTEEELLAPVDYLIEKFKEI
                     KIAKKDKPENAINWVMGQLHKQALGNVDPAVLHSRIKQRIS"
     gene            3590..4993
                     /gene="gatD"
                     /locus_tag="PLW22_09615"
     CDS             3590..4993
                     /gene="gatD"
                     /locus_tag="PLW22_09615"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOU74999.1"
                     /translation="MNNQKIMEDIFQGYKGRALEVLKKFNVRVWGKAIIETTRGEFQG
                     TVLPRAENDDDTHIVLKIATGYNIGIDIFTIKAMKEVGYYKATYKIPEKEFPYTPNLP
                     KVKLLGTGGTIASRLDYRTGAVIPAFSPGELYGAVPELADICNLSTEKLFAVFSENMG
                     PEQYKALAIAIGKEIENGIDGIIIGHGTDTLSHTAAALTFMVQNSPIPIVLVGSQRSS
                     DRPSSDAALNLMHAAYTAGHSDIAEVMVCMFGPTSDEYGFLHRGTRVRKMHSSYRSTF
                     RTIGDTPLATVSLKDGVKPIKDTYNHRRKDRKAKILPYFEEKVSIVYYYPNMKPDIID
                     SLVDNGYRGIIIAGTGLGHVNKPLYPAIERAKKKGVHMFMTVQTLWGYAHMYVYDTGR
                     DLMAKGIIPAENMLPETAYIKLGWVLGQTDDPEEVKRLMLTPINDEITPREPYNGYLI
                     YQGGVPEVEEFIRTFRK"
     gene            complement(6286..7920)
                     /gene="lnt"
                     /locus_tag="PLW22_09620"
     CDS             complement(6286..7920)
                     /gene="lnt"
                     /locus_tag="PLW22_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOU75000.1"
                     /translation="MQLKNLFKSNFFLMLLSGLLLALPWYEGLPSYTIFFGFVPLFVS
                     YYRLVEQKTGFWKIFGFVYLSFFTWNILATWWIYNATVIGAVLAVLITALLMTIVFSI
                     YHFIHINTSKTIGAFAFITFWIAWEQFFHNSEITWPWLTLGNSLGNHPMVIQWYEYTG
                     VLGGSLWILVVNFFILSQITAYRERVYGRIAWFFSDVLLFFTIPFALSLYIYFNHEEK
                     GEKVNVLIVQPNIDPYNEKFSGLTNEQQMDIMLNLAEENIDSTIRYVVCPETAIDDRI
                     WEHQLADNESVKRLKAFVDRHPGVMWITGLVSLKLYEPNEPVSHTARKLPYDERYYYD
                     RYNSAIQVDTLFNLPIYHKSKLVVGVEMMPYPQYLKFLEKLSIDLGGITGSLGTQPDR
                     GVFRSTDGKYGVGPIICYESVFGEYVAGYVQNGANLLFVITNDGWWGDTPGYRQHLSF
                     SRIRAIEMRRSIARSANTGISAIINQRGEVIDSLGWWKRGVIKGSVYANNATTYYARH
                     GDYIGNISAFMALLSLAYGISRFLMRRKKQESMSFS"
     gene            8022..>8974
                     /locus_tag="PLW22_09625"
     CDS             8022..>8974
                     /locus_tag="PLW22_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU75001.1"
                     /translation="MALPEIHPYIGGEFISTNETYHVHCPWDNSPIAKVHLAGEKELE
                     QAILLAQQAERPMAELAAFEKHEILMRIAQGIKENRNSFAKTLALEACKPIKLAYGEI
                     DRAIQTFIIAAEEAKRPPAELIRIDWTPQGKGKEGLVKCFPVGLIAGIAPFNFPLNLS
                     VHKIAPAIAAGCPIILKPARSTPLSALKLAEIIDSTPLPKGALSILPTNREVGSKLVT
                     DSRFALLSFTGSPQVGWNMKNQAGHKRVVLELGGNAGVVVTPSANLEQAVSKCLSGAF
                     AYAGQVCIHTQRIFVHQAVFTAFTEKFLQGTANLKNGHPLDT"
BASE COUNT         2594 a         2090 c         1986 g         2304 t
ORIGIN      
        1 aaaggcaagc catgtatgaa catgtggtta tcagaacccg aaaaggattt tacacaccca
       61 cataccacgg cattgactgg aatttcatgg cagaacacta ccggaaattc ctccctcata
      121 taggaaacgg gtttgagttt gcagagctgc tcagcgagct gcttggcgaa cttaacgtat
      181 cgcattcagg tgcacgcttc tacctcaaca accccaatgg cgaccaaacc gcttcgcttg
      241 gtattatacc cgatgtaacc tatcaagggc aaggcattag ggttgatgag attctgcttg
      301 gggggccact tgaccgtaag gaacttagcc tctctacacc cttcattatt actgcaattg
      361 acgggaatac cattggaact gataaggatt gggcatacta cctgaatcga aaagccgaca
      421 ggtatattct acttacctta acagaactaa gtaatggaaa ggaaagacag gttacagtga
      481 aacctacaaa cctatcccag gagtcccgat tgctataccg ccgttgggtt aagcgtaacg
      541 ccgatgaggt tgaaaagttg agcaacggta cattgggtta tgtccatatt ccagggatga
      601 gcgatggccc ttaccgcaat atttatggtg aaatgatggg aaaatattac gacaggtcag
      661 gcgtagtggt tgatacccgt ttcaatggtg gtggcgacct ggtttcggat ttggctatgt
      721 tctttaccgg cactaagttt atcacatacc ataaccacgt tcgcgaactt ggctccgagc
      781 caactttccg atggacaaaa ccaactgtag cacttgtaaa cgaagcaaac tacagcgatg
      841 gtagttgctt tgcttgtggc tacaagcaat tgggtattgg gaaacttatt ggaatgcctg
      901 ttcctggcac atgtagcttt gctggttggg aaacacttca ggataatcaa atccgctggg
      961 gtatggtacc ggtcagtgcc aaagatatgt atggtgtttg gatggaaaat gttgaagcaa
     1021 ttcccgatta cgtgataatg aatgaaccat cagttgcacc taagggacgc gaccagcaac
     1081 tcgaaaaggc cattaaagct ttaatggaag caactgtaaa gtagtgtttc aatctttcta
     1141 agagatttgg ctcaggctat gcctgagctt tttcgtttct agaccgctgc tgtcccatcg
     1201 aaattcatta gggttaactt cattgccaca ccttttcaag cctgacttta tttgatttta
     1261 gcataaaacg accgccccaa aaggggcggt cgttttttta gattggattt tggtttggtt
     1321 ttagattaca ttttgaatgg tttcaaaata attgcaaaac cagagaaaaa catttaacta
     1381 gcatcaaaca ctatgccact agcaatcatt tcaatttaaa attccgataa atcatcaaca
     1441 aaaaaccact aaaaagttaa ttttctgtta cttaatgatt atacgcaatt tttcaagcaa
     1501 tcacatagca ctgataaatc atttggaaat tcctagcagt tttttaactt agaaggctgt
     1561 taaccaaccc acacctaaac caaaatggcc aaaaaacaaa cgcccggtaa gcatttcgac
     1621 ccacaaaaga actaccggga aaccatgaag gctattggct atgtaacccg agccgaggcc
     1681 acccctaagg attatgaacg cattggattc aaatcggggc ttgaagttca tcagcaactc
     1741 aaaaccaagc aaaaactatt ctgccgctgt ccggcaggca tttaccacga cgataatgac
     1801 taccatgcgg aaattattcg ccatatgcga ccaaccctta gcgaaatggg cgaatacgac
     1861 ggtactgccc ttatggagtt taaaacccga aaggagattg tttaccgcat aaacaacaaa
     1921 accacctgca cttacgaaat tgacgacacg cctccttttc ccataaaccg agaggcgctg
     1981 gaaatagctc ttgaaatatc attgctttgc aagttaaaca ttgtgggcga ggttcacatt
     2041 acccgaaagc aatacctgga cggttccatc ccaaccggat tccaacgaac cgccattctg
     2101 ggtgttgagg gtcagattca gctaaagaat aaaaaggttg gactaatcca gctcagtatt
     2161 gaggaggatg cctgtcgtga aatctcagat gttgggcatc gtcgcatata caaaaccgac
     2221 aggttgggca tgccgctaat tgaaaccgtt acgcatcctc agtgccttac tcccgatgag
     2281 ctccgcgagg ctgctgacta tattcggttt ctgaaccgta gtaccggcaa ggttcgtact
     2341 ggtattggtg ctgcccgaca ggacacaaac gtaagctgcg agggtggaac acgtgtggaa
     2401 atcaagggag tttcgcataa caagtggcta ccaatgcttt cgcataatga gtgtttcaga
     2461 cagtgggcgc tactccacat cagaaaattg cttttggagc gtgtgccttt gcccgaaaag
     2521 tgggtaatgc gccatattga aatcgacccc tactcacaag gaattgagca tgcggttgca
     2581 aataggtttg ccgatatggg ttacaaggtt gttgcaattg cccttccggc atttcatagc
     2641 atactctcgc atttcaccca gcccggaaag tgcttcacca atgagctagc tgataggctc
     2701 aaggttatag cctgcattga acgacccaac atcactagta atgaggattt ggaaattccg
     2761 ctttctgaaa gcgacactac acgtttagca tcgctgctca aagcctcacc cgacgatgca
     2821 attgtactta tttggggccc cgccaacgat atcccaacag ctattgaaac cattgaagag
     2881 cggtgccgca tggcctttga gggggttcca cgcgaaaccc gccgcccgct tgccgatgga
     2941 acaaccattt ttgaacgcgt tctacccggt gccgaccgca tgtaccccga taccgactca
     3001 gcccccattc ctctggacga ggcctatatt gagaaattgc gtgagcgatt acctatcgac
     3061 atttggcacc gtttccaaca gcttgcccaa atgcaaattc ctgaggatgc ccatcccttc
     3121 ctgttaaggc gaaacctggt tccggttatc gaggaaattg ttggtttggg attcccttcg
     3181 cgctatgtag gttcactgct tgggcataag ctgaaacgaa ttgaagggaa ataccctaaa
     3241 cataaggatt ttaactactc ccgaattgta gaactattcc gtttcattaa agagaacaag
     3301 ctgcatcact ccatagctaa gcttatgctt ccggttgttt accagtaccc caacatggat
     3361 ttcaactcgg tactaaccag tatcaacttt aagcgaaaga ccgaggaaga actgcttgct
     3421 cctgtagatt acctcattga aaaattcaag gagataaaga ttgccaaaaa ggataagccg
     3481 gagaatgcta taaattgggt aatgggacag ctgcataagc aagcactggg gaatgttgac
     3541 cctgccgtat tgcactcaag gataaagcaa cgaattagct aaaaactcaa tgaataacca
     3601 aaagattatg gaagatattt ttcagggata taaaggccgt gcgcttgaag ttctaaaaaa
     3661 gttcaacgtt agggtttggg gaaaagccat cattgaaacc acccgaggtg agtttcaggg
     3721 tactgtttta ccccgtgccg aaaacgacga cgacactcac attgtgctaa agatagcaac
     3781 gggttacaat attggtatcg acatttttac aattaaagca atgaaagagg tggggtatta
     3841 caaggccacc tataaaattc cagaaaagga gttcccatac actcctaacc ttcccaaggt
     3901 taaactgctg ggaaccggag gtactattgc atcgcgactc gattaccgaa ctggagctgt
     3961 aataccagca ttctcgcctg gcgaactata cggcgctgta cccgaactag ccgatatttg
     4021 caacctatca acagagaagt tatttgcagt attcagtgag aacatgggcc cagaacagta
     4081 caaggcgctt gcaatagcca ttggcaaaga gattgaaaac gggattgatg gaataataat
     4141 tggtcatggc accgacaccc ttagtcacac tgcagcagca ctcaccttta tggttcaaaa
     4201 ctcaccaatc cccattgtac tggttggctc ccagcgaagt agcgaccgcc ccagcagcga
     4261 tgcagcgctt aacctgatgc atgctgcata cacagcaggc cattccgata ttgccgaggt
     4321 aatggtatgt atgtttggcc ccacctccga cgagtacgga ttccttcaca ggggcacaag
     4381 ggtacgtaaa atgcactcct cgtaccgcag caccttccgt accattggcg atacgccgct
     4441 ggcaaccgtt agcctaaagg atggcgttaa acccattaag gacacctaca accaccgccg
     4501 taaggacaga aaagccaaaa tacttcccta ctttgaagaa aaagtatcga tagtgtacta
     4561 ctacccaaat atgaaacccg atattatcga ctcattggtc gataatggat ataggggtat
     4621 aataattgcc gggacaggac ttggccacgt taacaaacct ctataccccg ccattgagcg
     4681 ggctaaaaag aagggtgttc acatgttcat gaccgttcaa accctttggg ggtatgccca
     4741 catgtacgtg tacgacaccg gtcgcgacct gatggccaag ggaatcatcc ccgccgagaa
     4801 catgctcccc gaaactgcct acatcaaatt gggttgggtg cttggccaaa ccgatgaccc
     4861 cgaggaggtg aaacgcctga tgcttactcc cattaacgat gagattaccc cacgtgaacc
     4921 ctataacggt tatctcattt accaaggtgg cgtgcccgag gttgaggagt tcatcaggac
     4981 gttcaggaag taaacaaggt tttaaatgtc acacaataac tgagatttga tgcctatggc
     5041 aaatctgtgc cattttgtgc aatgtttttt ctacaaggac taaactaaac agttcgccca
     5101 ttgttacaat tcctgtttta atgttgttta gttaaagtta ttgtcatgct tagcgcaagc
     5161 aaaacatcac gctgtcgtgc tgagtcccga attatatagg gttagacttc gccgaagcat
     5221 cttattgctc ttttcacttc aatcctttga ggagatccct cggccttgtt tgggatgaca
     5281 aaaaccctat cttcctttct gctcccttgt catgctgagc tagctgaagc atctctttac
     5341 ctttaatcct tgttattttt tgtgtaaacc gctctttgcc atacttttct gttgcgatgc
     5401 aacgtttcag tgtaactctt tggcaaaggt atagcgtgca tcgttttaga gcaggagtag
     5461 ccatggagtt acaaatatta cgcccctacg gggctggaag cgttgtttgt tgttcgtgaa
     5521 gctattgcca tgttgagcta agcgaaacgt cacatgagat cgattagaga tccttcgcta
     5581 cgctcaggat gacaaatgaa gttattcgtg taggttgctc gttgttcgtg ggatacgacc
     5641 tcaccccaac ccttttccct gagagggaat ggagcggaag aagcaagcag aggcataaaa
     5701 gcccgacccg gcaatgggtc gggggtggaa cgtggcggtg ggtttttgta agcgatacca
     5761 catgagtcac gccacaggcg tggcgaatag tatcgcgcgg cagttccacc cgcggggtac
     5821 ccatcgggtc gggcttttca gccttgattt tctttggttc tttcttgtat caagacaaga
     5881 aagaacataa aaaacgctct ttgtaatacc tttctgttgc gatgcaaagc ttcacctttg
     5941 ctctttggaa aatgtattgc gtgcatcgca aaataatttt aacacctcaa taccctgggt
     6001 taaaacccag ggctattgtg atttgccctt taagggcgat ttatgtgtgc gtctaaagac
     6061 gtgtgctaac tagtgagttc ttaaatctta aatcgagatc cctcgattac gctcgggatg
     6121 acaatggagt tttaaatgtt ttgctcctag gggctggaag cgttgttcgt tgttcgtgca
     6181 atcgttgttc ttgaagctat tgccatgttt agctaagcga aacgtctcat gggatcgatt
     6241 agagatcctt cgctgtgctc aggatgacag gcatgtatat ttttgttaac taaacgacat
     6301 tgattcctgt ttcttccttc gcatcaggaa ccttgaaata ccataggcta gtgagagtaa
     6361 agccatgaac gccgagatat ttcctatgta atcgccatga cgggcatagt atgtagtagc
     6421 attattggca tatacagagc cttttattac tccccgcttc caccaaccaa gggaatcaat
     6481 aacctcgccg cgctggttta tgatggccga tataccggta ttggcggaac gggctattga
     6541 acggcgcatc tcaatggcgc gaatgcgcga gaacgagagg tgttgcctgt agcccggcgt
     6601 atcgccccac caaccatcgt ttgtgataac aaagagcagg tttgctccat tttgcacata
     6661 acctgccaca tactccccaa aaaccgattc gtagcagatt attggcccta cgccatactt
     6721 accatcggtt gaacggaaaa cacctctatc gggctgtgtt cctaagcttc cggtaattcc
     6781 ccctaagtcg attgaaagtt tctcaagaaa cttaaggtat tgcgggtagg gcatcatctc
     6841 aaccccaaca accagtttgg atttgtggta aatgggcaaa ttgaacaaag tatcgacctg
     6901 aatggctgag ttatacctat cgtagtaata cctttcatcg taaggcagct ttcgggcagt
     6961 atgcgacact ggctcgttgg gctcataaag cttaagcgat accaaaccgg ttatccacat
     7021 cacaccggga tgcctatcaa caaaggcttt cagcctttta acactctcgt tatccgctag
     7081 ctgatgctcc caaatgcggt cgtcgatggc cgtctcagga cataccacat aacgaatggt
     7141 actgtcaatg ttctcttcag caagattaag catgatgtcc atttgctgct cgttagttaa
     7201 cccgctgaac ttttcattgt agggatcaat gttaggttga actatgagca cattcacttt
     7261 ttcgcccttc tcctcgtggt taaagtagat gtaaagcgac agggcaaaag ggattgtgaa
     7321 aaagaggaga acatcggaaa agaaccatgc aatgcgacca taaactctct cgcggtaagc
     7381 agtaatctgc gaaagaataa aaaagttaac cactaaaatc caaagcgaac ctcccaacac
     7441 acctgtatac tcgtaccact ggatcaccat aggatgatta cccaggctat tgcctagggt
     7501 tagccatggc caggttattt cggaattatg aaaaaattgc tcccaggcaa tccaaaaagt
     7561 tataaacgca aaagcaccaa tggttttact cgtatttata tgaatgaaat ggtaaatgct
     7621 gaaaacaatg gtcattagca aagccgtaat caaaacagcc aatacggctc caatcacagt
     7681 agcgttataa atccaccagg tagctaaaat gttccaggtg aaaaaagaca ggtacacaaa
     7741 cccaaatatc ttccaaaaac cagttttttg ctcaactagc ctatagtatg atacaaaaag
     7801 gggaacaaag ccaaaaaaaa tcgtatagct tggcaaaccc tcataccatg gtagcgctaa
     7861 aagcaacccg gaaagaagca tcagaaaaaa attagattta aacagattct ttagctgcat
     7921 ggtggatgtt attttaaaaa cgatgtgtaa agataaacga tttgccgttc ttactaatcc
     7981 aattaaagct atttttgggt gtaatttaaa atttgcttgc tatggctcta cctgaaatac
     8041 acccatacat tggaggtgaa ttcatttcca caaacgaaac ctaccatgtt cattgtccat
     8101 gggacaattc tcccattgcc aaggttcatc ttgctggaga gaaagaactt gaacaggcaa
     8161 tactgctagc acagcaagcc gagcgaccaa tggcagaact ggcagccttt gaaaaacacg
     8221 agatattaat gcgtattgct cagggaataa aggaaaaccg caatagcttt gccaaaactc
     8281 ttgcccttga ggcttgcaaa cccataaagt tggcctatgg cgagattgac agggccatac
     8341 aaactttcat aattgcagcc gaggaagcta aaaggccacc tgcagagctc atacgaatcg
     8401 attggacacc tcaaggaaaa ggtaaagagg gactggtaaa gtgctttccc gttggcctaa
     8461 ttgcaggtat agcaccattc aacttccctc taaaccttag cgttcataaa atagctcccg
     8521 ccattgcggc tggctgtcca attattttaa agccagcccg ttcaacaccc ctatcggctt
     8581 taaaacttgc cgagattatt gactctaccc cactacccaa gggtgcactt tcaatactgc
     8641 ccaccaaccg tgaggttggt agcaaactgg taaccgactc caggtttgcc ttgctatcat
     8701 ttaccggttc accccaggtt ggttggaata tgaaaaatca ggctggacat aaacgcgtag
     8761 tgcttgaact tggaggcaac gcaggtgtag tagtaactcc atcagccaac cttgaacagg
     8821 ccgtttcaaa gtgcctgagc ggtgcctttg cctatgcagg gcaggtgtgc attcacaccc
     8881 aacgaatttt tgtacatcaa gcagttttca ccgccttcac cgaaaagttc ctacaaggca
     8941 cagcaaatct taagaatggt cacccgctgg atac
//
LOCUS       DAOOWJ010000106         8959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_7754_length_8959_cov_6.733041, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000106 DAOOWJ010000000
VERSION     DAOOWJ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7754_length_8959_cov_6.733041"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLW22_09630"
     CDS             complement(<1..252)
                     /locus_tag="PLW22_09630"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU75002.1"
                     /translation="MKKILSVLIVSMLALNLIAANEDAKKESAEATSNMVSTISGKVV
                     DKTTGEALAGVAVKLDENTVVYTDFEGNFTINLTQPKAKL"
     gene            complement(430..3240)
                     /locus_tag="PLW22_09635"
     CDS             complement(430..3240)
                     /locus_tag="PLW22_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU75003.1"
                     /translation="MVKTNNKIVLLLLSGFFVVFEAWAQKGSIEGVVYDRSHNETLVG
                     ANVIIEGTTTGTITDFDGRFTLSNLNPGTYNVLVSFISYQPVILNNIKVEPNKSTVVK
                     VELEEITTAIEGVTVTAARKTNTEVAMLSTIKASLTVASGISAQQITRSQDRDASEVI
                     RRVPGVSIIDDRFVVVRGLNQRYNNAWINNASTPSSEADQKAFSFDVIPSAMIDNMVI
                     YKSPSPELPADFTGGFIKISTKNMPDENFLSIGYATGFNTSTNYGNFKQITKSKTDWL
                     GFDDGTRNLPADFPSTLKGLTNSQLAGLGQKLNTSWAPQSVQAVPDQKFNLSFGRKWQ
                     KGNKRLGNITSLSYSNTFETEEVNHSEYQVQLVPGQEPPFNHNYTDSIFTRKVKIGLL
                     HNWSFFTGKGSKIEFRNLFNLIGKSNAIIRNGLNGHEGFTIRSYQDNFQNRTTYSSQL
                     GGEHRWGDNQKNKLDWVTGYALSYRNEPDLKQLRTTLQDEPLIPHYNEYYAAVGITPS
                     VSDAGRVYMKLVEHIASLGVNYERKLKIGNWEPTLKSGTYGEFKARNFDARILGFAKN
                     SSYTESVWLPIDQIFSKENINTSPDGFLLRESTSKSDSYNVSSILGSAYIALNTNITK
                     KLSFYGGLRAEWMNQILNSYDRYGRPVKVEDDGIDIFPSANITYNFNEKNLIRLAAGI
                     SINRPEYREIAPFYFYNFTDEAEFVGNTNLKNCLIHNYDLRWELYPNTGETFSIGLFY
                     KKFNGPIELVFKPAGARPLFTYNNAEGAYSFGVEVDLRKSLQAIPLLKDFYMVLNASY
                     IHSKVIFPEGSIFRNRPMQGQSPYVVNAGIFYQNDKNNLSVSVQYNVIGDRILAVGIP
                     FQNQDQDIPDYYEKHQHLVDITVSKEFGKKVEIKAGVKNLLNQKYNTYQPFIGSNNND
                     MTMNNKLYNQGITFSLGANLRF"
     gene            3426..4118
                     /locus_tag="PLW22_09640"
     CDS             3426..4118
                     /locus_tag="PLW22_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU75004.1"
                     /translation="MNVSRYRILIVDDEPDIVEFLSYNLKKEGFQIQTASNGREAIER
                     AIDFRPHLILLDVMMPDMDGIETCEQLRKQPSTSNTLIAFLTARSEDYSQIAGFDAGG
                     DDYITKPIKPKVLISRIKALLKRSGTLIDDAESPDTIPAEGIYIDKERYSVYVNGNEL
                     SLPKKEFELLALLYSKPQKVFTRDEIYQAVWGDDIVVGDRTIDVHIRKLREKLGDDYI
                     KTVKGVGYKFTS"
     gene            4253..4660
                     /locus_tag="PLW22_09645"
     CDS             4253..4660
                     /locus_tag="PLW22_09645"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOU75005.1"
                     /translation="MQIAIWKPEYSVGVKSIDEDHQKLFDLLNQLFESMTKGKGKEII
                     NDIISELERYAVFHFGREESYFRVTNYPFALQHIKEHQYFKQKVAELKKDVALNKGMV
                     APDVLGFLSDWLKNHIAKSDKAYEDHLKKHAVI"
     gene            complement(4794..>6229)
                     /locus_tag="PLW22_09650"
     CDS             complement(4794..>6229)
                     /locus_tag="PLW22_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOU75006.1"
                     /translation="ALSFAGNDLVNFIGVSMAGLKAYQIHAANPDIAPESLRMTELAG
                     EVSTNFMYLFIAGLIMVITLWTSRKARTVTDTEVGLARQDAGMERFGSTQFSRIIVRI
                     ARRFAEHVDKVTPRFIRKFVAKQFDTTKAPRYSNPKDAPSFDLVRASVNLVMASILIS
                     SATALKLPLSTTYVTFMVAMGTSLSDKAWGRESAVYRITGVITVIAGWFFTAFVTFTI
                     AFGIATALYFGKAPALIILLAIAIGLLIKSHLLHKKKTKQLTDEEIVDEVSIVNRCTN
                     ELRKYLKQSIKVYNRTIDGLTTENRRQLKEVNEEVEALNMEAKKLKYNVYYVLKQLEA
                     DSIETGHYYVQVIDYLRELAHSLEFVSRPSLQHVENQHKGLTREQAAELKDLSNQLSD
                     LFDDILEMIKQNDYTRVPDAIKKQQDILEDLNKIRKKQVKRIKQGETGTRNSVLYLGL
                     LNEIKNLLLHTINILKAQRDFILNNIE"
     assembly_gap    6230..6329
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6330..6856)
                     /locus_tag="PLW22_09655"
     CDS             complement(<6330..6856)
                     /locus_tag="PLW22_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOU75007.1"
                     /translation="METYYLFIVGILFLLAASDLMVGVANDAVNFLNSAIGSKVAKRH
                     WILLVASAGVLVGSMFSSGMMEVARKGVFRPENFYFTEMMVIFLAVMITDVIVLDFFN
                     TFGLPTSTTVSLVFELLGSAVAVSIFKIMNTEGETISNLGNYINSGKALAIISGILSS
                     VVVAFVVGMVVQYIAR"
     gene            complement(6966..7694)
                     /locus_tag="PLW22_09660"
     CDS             complement(6966..7694)
                     /locus_tag="PLW22_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU75008.1"
                     /translation="MVKAVIVDDEIHARESLSRMLNVYYPSITVIGKAESIKSAYDLI
                     KTQCPDIVFLDMQLTDGHGFDLLKMFENIPFKVIIISAYQEYAVKAFKFSAVDYLLKP
                     VDPDDLVKAVDIALAQALNDKQQERLNALIENTRNIGKKDKLLALRNSDGTYIVNTSE
                     IVRCESDNNATIFYLVDGNQVRVNRTLKEFDEMLNECGFLRCHQSHLVNQRHIEKISR
                     FPSSLITMNNGDVIPVSFRKRKSI"
     gene            complement(7694..8443)
                     /locus_tag="PLW22_09665"
     CDS             complement(7694..8443)
                     /locus_tag="PLW22_09665"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOU75009.1"
                     /translation="MFISSTPQVTLLVIHSVVFAIAIIYLITSRKRLKERILRYEQQV
                     LLSQMNPHFVFNSLTAIQSYIFRNEPHTASKYLASFAKLTRLILENSRCELCSLEREI
                     ATLKLYLDLQKLRFEGRFDYTITVAEQIDPDATLIPPMLAQPLIENAIEHGLSGINWA
                     GKIEIRYTLTKPKQLVIEVEDNGIGIEKSREVQESKGKQYRSLATEITKERIKNLKKL
                     GNSGIKLNITDKSTLSGSTSGTIAQIVIPLK"
     gene            8539..8949
                     /locus_tag="PLW22_09670"
     CDS             8539..8949
                     /locus_tag="PLW22_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75010.1"
                     /translation="MKRVVSIILLGLGVGMMLVHSLVPHHHHNGEVCFVEAAQMCCQH
                     SNGNHHDSPHSHSHDQSCNLLNDLTQAEKFRVIDISQVISNLNVVKDFFANLNRCIDT
                     AVRLELSQRIYISGISKTWQIPIISCHTLRAPPF"
BASE COUNT         2530 a         1763 c         1800 g         2766 t
ORIGIN      
        1 taattttgcc ttaggctgag taaggtttat tgtaaagtta ccctcaaaat cggtataaac
       61 aactgtattc tcatcaagct taaccgctac accggccaga gcctcgcctg ttgttttatc
      121 aactaccttt ccactaatgg tgctaaccat gttggaagta gcttcagccg attccttttt
      181 agcatcttcg ttagcagcta taaggtttag tgccaacatg ctaactatta aaactgacag
      241 tatttttttc attgctttat ggttttctca tttaactact gcaaagtaaa tactccaata
      301 ttgcctcatt attacccaaa aatgatttgt atgttaagtt tatgtaaagt gtataaatca
      361 tgctaggaaa aagaaaagcc ggatgttaaa actaatctaa acatccggca ttggtggtta
      421 aaacctacac taaaacctaa gattggcacc aagggaaaat gttataccct ggttgtataa
      481 tttattgttc atagtcatat cgttgttgtt tgaacctatg aaaggctggt atgtgttata
      541 tttttggttt aaaagatttt ttacgccagc tttaatttca acctttttac caaactcttt
      601 cgatacagtt atgtcaacta aatgttggtg cttttcgtag taatcgggaa tatcctggtc
      661 ctggttctgg aaaggaattc caaccgccag aattctatcc cctataacat tgtactgcac
      721 ggaaacgctt aggttgtttt tatcgttctg gtagaaaata ccagcattaa ccacgtatgg
      781 agattggccc tgcatgggtc tattcctgaa aatacttcct tcagggaaaa taaccttact
      841 atgtatatac gacgcattca agaccatgta aaaatcttta agcaatggaa tggcttgcag
      901 ggacttgcga aggtcaactt ctacaccaaa gctataggcc ccttcagcat tattgtaagt
      961 gaagaggggt cgagcaccag ctggtttaaa aacaagttca attgggccat taaacttttt
     1021 ataaaaaagc ccaattgaaa aggtttcacc agtattggga taaagttccc agcgtaagtc
     1081 gtagttatga atgaggcagt tcttaaggtt tgtgttcccc acaaattcag cttcatcggt
     1141 aaagttatag aagtagaatg gggcaatctc gcggtattca ggacggttta tagagatacc
     1201 tgctgccagc ctgataaggt ttttttcgtt aaagttatag gtaatatttg ctgatgggaa
     1261 aatatcaatg ccatcatcct caactttaac tggcctaccg taacggtcgt aactgttcag
     1321 aatctggttc atccattccg ccctaagtcc cccataaaat gataattttt tggtaatatt
     1381 tgtgttaagg gctatgtatg ctgatcccaa aatggatgaa acgttgtatg agtcagattt
     1441 tgaggttgac tctctgagca aaaacccatc ggggcttgtg ttgatgtttt ccttactaaa
     1501 aatctgatcg ataggtaacc aaaccgattc agtgtacgat gaatttttgg caaatcccag
     1561 aatgcgtgcg tcaaagttgc gtgccttaaa ttccccgtaa gtaccgcttt ttagggtagg
     1621 ttcccaattc cctattttta attttctttc gtagttgact cctaaactgg caatatgctc
     1681 aacaagcttc atgtaaactc tacccgcatc gcttactgag ggggtaattc caactgctgc
     1741 gtaatactca ttgtagtgtg gaataagggg ttcatcctgc agggtagtgc gtagctgctt
     1801 caggtcaggc tcgttcctgt atgaaagagc atagcctgta acccaatcta atttattctt
     1861 ttgattgtcg ccccagcggt gttctccacc aagctgtgat gagtaggttg ttcggttttg
     1921 aaagttatcc tggtaggaac gaatggtaaa accttcatgc ccatttagcc catttcggat
     1981 tattgcattg cttttaccaa ttaggttaaa aaggttacga aattcaattt tactcccttt
     2041 accagtgaaa aacgaccagt tgtgaagcaa gccaattttt acttttcggg taaatatact
     2101 atcggtatag ttatggttaa agggaggttc ctggccaggc actaactgca cctggtattc
     2161 actatgatta acttcttcag tctcaaaggt gttgctatag gaaagtgaag taatgttacc
     2221 caagcgttta ttccctttct gccattttct tccaaaggaa aggttgaact tttggtcagg
     2281 tactgcttgt acactttggg gcgcccaact ggtgtttaat ttctgcccta atcctgctag
     2341 ctgtgagttt gttaagcctt taagtgttga tggaaagtcg gcgggtaaat tccttgtacc
     2401 gtcatcaaag cccagccaat cggttttact tttggttatc tgcttaaaat tgccatagtt
     2461 tgtgctagtg ttgaacccag tagcataacc aatgctaaga aagttttcat cgggcatatt
     2521 tttcgtagag attttaataa atccacccgt aaagtccgct ggtagctctg gcgaggggct
     2581 tttgtaaatc accatgttgt caatcatggc acttggaata acatcgaagg aaaaagcttt
     2641 ttgatcggct tcgctggaag gagttgaagc attgttaatc caggcgttgt tataacgctg
     2701 gtttaaccct cgcaccacaa caaacctgtc gtcaataatt gaaactccgg gaacacgtcg
     2761 tataacctcc gaagcgtcgc gatcctgaga acgggtaatt tgctgtgccg atattccgct
     2821 tgccacagta agacttgcct ttatggtgct taacatggca acctcagtgt ttgttttacg
     2881 tgcggctgtt acagtaaccc cttcaatggc agtggttatt tcttcaagct caacttttac
     2941 tactgtactt ttattaggct caactttaat gttattcaaa attacgggtt ggtaggagat
     3001 aaaagaaacc agaacattgt aagttcccgg attaagattt gataaggtaa acctaccatc
     3061 aaaatcggta atagtaccgg tagtggtacc ctcaattatt acattggcac ccacaagggt
     3121 ctcgttgtgc gacctgtcgt aaaccacacc ttcaattgat cctttttgtg cccaggcctc
     3181 aaaaacaaca aaaaaacctg aaagcaaaag caatacaatc ttattattag ttttcaccat
     3241 agtttgttta gtatttccta cttttttaca gctacaaagt aagccacatt aaatggcata
     3301 gattttaatt ccacgttacc gttttgttaa tctaacccta acccgttttg taaaaaagtt
     3361 aactttgagt taactttaaa cacatgctaa gccttttata tttgtaaaaa caaaaattta
     3421 aaggaatgaa tgtatcacgg tatagaatcc tgatagttga tgatgaaccc gatattgtag
     3481 agttcctcag ctataacctt aaaaaggagg ggtttcaaat tcaaactgcc tccaatggcc
     3541 gtgaggccat cgaaagggct atcgatttcc gaccacacct aatactcctc gacgtaatga
     3601 tgcctgacat ggatggaatt gaaacctgtg aacagcttcg taaacagcca tcaacttcaa
     3661 atactttaat agccttcctt acagcgcgca gcgaggacta cagccaaatt gcaggattcg
     3721 atgctggggg cgacgattac atcaccaagc caattaagcc aaaagtgctg ataagcagaa
     3781 taaaggcatt actcaaacga agcggcaccc tcattgatga tgctgaaagc cctgatacta
     3841 taccagccga aggaatttat atagataagg aacgctactc cgtttatgta aatgggaatg
     3901 agctatcgct cccaaaaaag gagtttgagc tacttgcttt gctttacagc aaaccccaaa
     3961 aggttttcac ccgcgatgaa atttaccaag ccgtttgggg cgatgacatt gtggttggcg
     4021 atagaactat tgatgttcat atccgcaagc tacgcgaaaa acttggtgat gactacatta
     4081 aaactgtaaa gggggttggt tacaagttta cttcgtaaat caagttgatg ttgtatatta
     4141 tttgccccca atttgtaaaa ttaccctaaa cccaattgga gtaatgtttg taaagatata
     4201 tttttgaaaa aataattttt aatttgatag ctcaattttt gttaccattt atatgcaaat
     4261 agcaatttgg aaacccgagt atagtgttgg tgtaaaaagt attgatgagg atcaccaaaa
     4321 actcttcgat ttacttaacc agctttttga gtcaatgact aagggtaaag ggaaagaaat
     4381 aataaatgat attatttcag agctggaaag gtatgcggtt ttccattttg ggagagagga
     4441 gtcgtatttt agggtaacta actacccatt tgctcttcag catattaagg agcatcagta
     4501 cttcaagcag aaagtagcag agcttaaaaa ggatgttgca ttaaacaaag gcatggttgc
     4561 tccagatgta cttggtttcc tgagcgattg gttaaagaat cacattgcca aaagcgataa
     4621 ggcctacgaa gatcatttaa agaaacacgc tgtaatttaa taaatacaat ttatttccat
     4681 tttaaaatgg ttgacacagg ttttatggtc ttgaattaat tttagttcaa tccataaaac
     4741 ctgtgtcatt tctattataa tgatattgtt tcttagcttt ataaaatggg ctgctactca
     4801 atattgttta gaataaagtc ccgttgagct ttcaggatgt taattgtatg taaaagcagg
     4861 ttcttaattt cgtttagcaa tccaagataa agcacgctat tgcgagttcc tgtttcaccc
     4921 tgtttaatac gttttacctg tttcttgcgt attttgttca gatcttcaag gatatcctgc
     4981 tgctttttga tagcatcggg aacgcgggtg taatcgttct gcttaatcat ttccagaata
     5041 tcatcgaaga gatccgaaag ctggttgctc aaatccttca gttcagcagc ctgctcacgg
     5101 gttaacccct tatgttggtt ctcaacatgc tgtaagctgg ggcgtgagac aaactccagg
     5161 gagtgggcaa gctcacgcag gtaatcaatt acctgaacgt aataatgacc agtttcaatt
     5221 gagtcggctt caagctgctt gagaacatag tatacgttat actttaactt tttggcttcc
     5281 atgttaaggg cctccacctc ctcgtttacc tccttaagct gccggcggtt ctcggttgtt
     5341 agcccatcaa tggttctatt gtaaaccttt attgactgtt ttaagtattt acgtaattca
     5401 ttggtgcatc ggtttacgat actaacttca tcaactatct cctcgtcagt taattgcttg
     5461 gtctttttct tgtgcaacag gtgtgattta attagtagac caatagctat agccagtaaa
     5521 ataattagtg cgggtgcttt gccaaagtaa agtgcggtag ctattccaaa agcaattgta
     5581 aaggttacaa aggcagtaaa gaaccatcct gcaataactg taattactcc tgtaatacga
     5641 taaactgcgc tttcgcgacc ccaggcttta tcgctcagcg atgttcccat agcaaccata
     5701 aaggttacgt aggtggttga aagagggagc tttagtgcgg tagccgaact gattagtata
     5761 cttgccatta caaggttaac ggaggctctt actaaatcga acgatggggc gtccttgggg
     5821 ttagagtatc ggggtgcttt agtggtatcg aactgcttag ccacaaattt tctgataaaa
     5881 cggggtgtaa ctttatcgac atgctctgca aaccttcggg ctattcgaac aattatcctt
     5941 gaaaactgtg tacttccaaa ccgctccata cctgcatcct gacgggctaa gcccacttcg
     6001 gtatcggtaa ctgttcttgc tttgcgcgat gtccaaaggg taattaccat tattaagcct
     6061 gcaatgaata ggtacataaa gttagtgctt acctcaccag ctaattcagt cattctcagc
     6121 gattcaggag cgatatcggg atttgctgca tgtatttggt aagcttttag tccagccatc
     6181 gaaacaccaa taaagttcac aaggtcgttc ccggcaaacg ataatgctan nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gtgcaatgta ctgaacaacc attccgacta
     6361 caaaggcaac aaccactgag ctcaatatcc ccgatataat tgctagggct ttaccactat
     6421 ttatgtaatt tcccaggttt gaaatggttt caccctcggt attcattatc ttgaaaatgg
     6481 aaacagcaac tgccgaacct aaaagttcaa acacaaggga aaccgtagtt gaggtgggta
     6541 gtccaaaggt gttaaagaag tcgagaacaa taacatcggt aatcattacg gcaaggaaaa
     6601 taaccatcat ctctgtaaag tagaagtttt ccggccggaa tacgcccttt cgggcaactt
     6661 ccatcatacc gcttgaaaac attgaaccta ctaatactcc tgccgaagca accaacagta
     6721 tccaatgcct tttagcaact tttgacccta tggctgagtt aaggaaatta acagcatcgt
     6781 ttgccacacc taccatcagg tccgatgcag ccagtagaaa aaggatacct acaataaata
     6841 aatagtatgt ttccattgta ttatagtttg acgcgcaaaa ttaccctttc aaatgcttgg
     6901 tttcttaaat gccctattac ttttaggtta acccaatgtt aagttaatgt taagtgggtt
     6961 aaaaatcaaa ttgattttct ttttctaaac gatactggaa tcacatcgcc attgttcatg
     7021 gtgattaatg atgaagggaa ccgggaaatc ttttcaatat gccgttgatt caccaaatgc
     7081 gattgatggc agcgtaaaaa tccgcattcg ttaagcattt cgtcaaactc cttgagagtt
     7141 cggtttaccc gtacttggtt gccatccaca aggtaaaaaa tggttgcatt gttatcggat
     7201 tcgcatcgaa caatttctga agtgttaaca atataggttc catcggagtt cctgagtgct
     7261 agtagtttat cctttttccc aatattcctg gtgttttcaa taagggcatt gaggcgctcc
     7321 tgttgcttat cgttcagggc ttgggctagg gcaatatcta cagctttaac aagatcatcg
     7381 gggtctacgg gcttaagaag atagtctaca gcgctaaact taaaggcttt gactgcatat
     7441 tcctgatagg cggaaattat aatcacctta aaaggtatat tttcaaacat cttcagcagg
     7501 tcaaacccat gcccatcggt taactgcatg tcgaggaata caatatcggg gcattgggtt
     7561 tttattaaat cgtaggcgct ttttatgctt tcagctttac ctataacggt tatagatggg
     7621 taatatacat taagcattcg ggatagtgat tcccgcgcat gtatctcgtc gtcaactata
     7681 actgctttta ccattacttt aggggtatta caatttgggc tatagttccc gaggtgctcc
     7741 cacttagggt cgatttatcg gtaatgttta gcttgattcc cgaattccca agctttttga
     7801 ggtttttaat acgttccttg gttatttcgg tagctaacga cctgtattgt ttccctttac
     7861 tctcttgaac ttcgcgcgat ttctcaattc ctataccgtt gtcttccact tcaattacca
     7921 gttgctttgg tttagttagg gtatagcgaa tctctatttt acctgcccag ttaatgcccg
     7981 acaagccatg ctcaatggca ttttcaatga gcggttgcgc aagcataggt gggattagtg
     8041 ttgcatcggg gtcaatttgc tctgcaacgg ttatggtata gtcgaacctt ccttcaaacc
     8101 ttaacttctg taagtcgagg tagagcttaa gggtggcaat ttctctctct aagctacaca
     8161 gctcacaacg agagttttca aggattaggc gggtaagctt tgcaaagctt gcgagatact
     8221 tactggcggt atggggttcg ttgcggaaaa tatagctttg aatggctgtt aaggagttga
     8281 atacaaaatg cggattcatt tgcgagagca aaacttgctg ttcgtatctt aaaatccgct
     8341 cttttagcct ttttctgctg gtaataaggt agattatggc aatggcaaac accacggaat
     8401 gaataacaag cagagttact tgtggagtgg aagatatgaa catggtgcac aattgtttga
     8461 tgcaaatata tgttttttgg ttaaaccatt aagtgggaat tcaatcgaaa agaataaatt
     8521 tgcagccaat tacttttcat gaaaagggtc gtttcaataa tactactggg gctgggtgtg
     8581 gggatgatgt tggttcattc acttgttccg catcaccacc acaatggcga ggtttgcttt
     8641 gttgaggctg ctcagatgtg ttgccagcat agcaatggta accaccacga ttccccccat
     8701 agccattccc acgatcagtc atgcaacctt ttgaatgacc ttacccaggc cgaaaagttt
     8761 agggttattg atatttcgca ggtaatctca aatcttaacg ttgttaagga tttttttgct
     8821 aatttaaata gatgtatcga tacagccgta aggctggaac tttcacaaag aatctacatt
     8881 tctggtattt ctaaaacctg gcagattcca attattagct gccacacatt aagggctccc
     8941 cctttctaaa tcgatttag
//
LOCUS       DAOOWJ010000107         8743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_8015_length_8743_cov_7.359461, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000107 DAOOWJ010000000
VERSION     DAOOWJ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8015_length_8743_cov_7.359461"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1170
                     /locus_tag="PLW22_09675"
     CDS             145..1170
                     /locus_tag="PLW22_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75011.1"
                     /translation="MKSKLTEWADLVEPVRSVPESERWGYRTKTTLSARFNGNIWEFG
                     MWRRDELVPIPNCPVHAPELNRVLALLADSFPREHSFALAYVVISGAQVVLVIKSKQE
                     PTLTWLSEDVAQGLASMGIEGIWYHMNPSTGRRIFEKSGWMLIWGKTRSTDYHGSLYG
                     PAAFQQLIPELYNESLEEAERFLNPNGNSALIDLYCGTGNSMRRWINRGANVIGAELG
                     GEAVECARLNAPQAMVLRGACRQRVPQIAEWTDKNRYQGKEILLYVNPPRTGLEPEVL
                     EWISKQGLPNRIAYLSCSPGTLSKNLNWLCTNGYRLVRCVPFDFFPQTHHVETLALLE
                     RVSADTY"
     gene            complement(1151..2632)
                     /locus_tag="PLW22_09680"
     CDS             complement(1151..2632)
                     /locus_tag="PLW22_09680"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOU75012.1"
                     /translation="MNFTTENFLLIGSLLLFISLIAGKTSFRLGVPTLIFFLFVGMLA
                     GTSFSGLVVFNDAITAQFIGVVALNFILFAGGFETNWFSIKPILWHGVTLSTLGVVLT
                     ALFSGLITWLVTDFSITESFLIGSIISSTDAAAVFSILRSKNLALKGNLRPTLELESG
                     SNDPMAYLLTIIFLGLATSPSLSFWEVVKLFLSQIIIGVSMGVGFGYASKYIINNIKL
                     EYEGLYPVLAITLMLLTFSITDFIGGNGFLAVYLSAILLGNSHIMHKKIILKMFDGLA
                     WLMQIILFITLGLLVNAGEIIPHIGNGLLIAFGLMFIARPLSVFISLSPYRMKNRERW
                     YIAWVGLRGAVPIVLATYPLIAGMAKAQEIFNIVFFITITSVLIQGTSLIRVAKWLRV
                     LLPQRVKQITPVDVMLRENISTEMVEVDIPDDSCVVGKRIVDLNFPEKSLIVLIQRND
                     RYISPTGSTVIEPNDKLIIVAEDKTSLKESFSCLSLDANTYQR"
     gene            complement(2637..3158)
                     /locus_tag="PLW22_09685"
     CDS             complement(2637..3158)
                     /locus_tag="PLW22_09685"
                     /inference="COORDINATES: protein motif:HMM:NF022766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HOU75013.1"
                     /translation="MRYSTLIIALALGVLFGCKHHEKQTVKLAKFDPVHIKVKNPVKK
                     VRAQTLYLPVYSSIPFAEGNSFYGLSAFMAVHNTDFNNPIYIISVYYLDTNGNLIKDF
                     LQSRVDTVMPMQSVNYFVPESDRSGVGANFILEWMADSAVNEPLVETIMVSLTSGQGV
                     SFLSEGKILNELY"
     gene            3236..4441
                     /locus_tag="PLW22_09690"
     CDS             3236..4441
                     /locus_tag="PLW22_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75014.1"
                     /translation="MKRHALLLALILLVAQAFACTTAIISGKATPDGRPLLWKHRDAD
                     DFNNKVVFEVGPRFKYLALINSSDPERHAWAGTNNAGFAIMNSASYNIKPKTDSSKVE
                     DLEGYIMKLALGSCATITDFAQMLDTLPKPWGVSANFGVIDAKGGAGYFEVNNFSYRF
                     YDANSTTDAPEGYLIRSNFSVSGEPNKGAGYIRFHTASNLFKEAKAANGLTHEFLLTK
                     ASVSLRQDITGIDLSKEVSKAEKIYPFRDFIVRYHSSSTVVVQGVLPKEPTDFATMWV
                     KLGFQPASVAIPLWVGAGGILPDIITAPDGLNAKLCDFSLKLKKDCFPLSSWTEGENY
                     VLLSKLINKEQTGIIQLTNIFDKEIIARTKTLYDKWTKFEFNPIEAYNFYLTLNRDVE
                     KFYKNHFDL"
     gene            4438..5127
                     /locus_tag="PLW22_09695"
     CDS             4438..5127
                     /locus_tag="PLW22_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOU75015.1"
                     /translation="MTTRKRLVVLTGAGISAESGIRTFRDMGGLWEEYDVMEVASIDG
                     WKKNPQLVLEFYNQRRRQLLTVEPNAAHKVLAEVQNLFNVFVITQNVDNLHERAGSKN
                     ILHLHGELTKARSTGNPSRIIDIGYNDIKWGDTVDGYQLRPHIVWFGEEVPMMNEAVK
                     LVQTAEIFAVIGTSLNVYPAAGLLDHAPKGCPIYLVDPNDINIWRRDVTVIKEKASVG
                     VPKLIEMIKNE"
     gene            5120..5731
                     /locus_tag="PLW22_09700"
     CDS             5120..5731
                     /locus_tag="PLW22_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOU75016.1"
                     /translation="MNRVLLLGFVLLCLGSTQMQGQSFRGGLLAGVAATQVDGDTYGG
                     YDKAGPIAGFWVERSKLSGWFYRLSFRFIQKGSYAKSRSTDIPDFYRMRLNYFDLPVL
                     AGYRFVNGFNVVLGLSGGYLSKATEENSLGPYPQEEVSTFKKFEIAGIAGMEYNYSSK
                     WRFGLNLSYSILPVRPYKDNISYRMNNGQYNRVIEFVSIYRIQ"
     gene            5731..6849
                     /locus_tag="PLW22_09705"
     CDS             5731..6849
                     /locus_tag="PLW22_09705"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOU75017.1"
                     /translation="MLLKRKLFFTLTLALFVLLHSGKAQEGRYSIDIKERPGFIIPHH
                     SHIAYFLKENVNAIQLNFGITTDGSKFWHKEFNYPIVGLGLYYSGLGNDTLYGKLTGL
                     YTFINRPFLDNTKRFNISNTLSVGLSYASKWHNPISNRSNMVLSTPLNVFFQYEIGCN
                     YRISTNHNVSASIGLTHASNGSVKEPNLGFNIFTTALGYRYSFGNPNPVRSELPDDIT
                     PFSSWNFGLFASIKSIDAFSGKQYGIIGLSAEKLYRFAPLSMAGLELTAYKDNSIPDL
                     VMDKSEDAVTLNKNDNLALTINATYLMGFNRIGIVFQPGIYLRNQYYGFGAFSNKVGL
                     RINIYDGLILGVAIKAHWFAQADFVEFGIKNSFNRHEK"
     gene            6839..7564
                     /locus_tag="PLW22_09710"
     CDS             6839..7564
                     /locus_tag="PLW22_09710"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOU75018.1"
                     /translation="MKNRIYLLILFFGIFYSCSLISDYTLGDMAERRVNLPATFEKID
                     IKGSFSITLLPDTVGFAVVKCHEKVMHDVEVKIQNNTLFLRENVKSRWLKDYPIIDIE
                     LHLTEIPMIEIHQPCKFTIPYTFKSHAFYLVDWGNYVDCDVNIEVDFLKIDVSGKSFG
                     TYRATGQAEHATLYARGAALFDLSQTQVKRCTANQGSTVKMDIWVTDNLMANITSTGD
                     IHLRGNPTIELNSQGKGELIRTP"
     gene            complement(7625..>8743)
                     /locus_tag="PLW22_09715"
     CDS             complement(7625..>8743)
                     /locus_tag="PLW22_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU75019.1"
                     /translation="KTDSKADRGGLIGSFFLALTTVIVSLSCVGPIVGALLVEAASGL
                     GAKPIVGMFGFSLAFSIPFTLFAIFPSWLNSLPRSGGWMNSVKVFLGFIVLAFSLKFL
                     LTIDQGYQLEMFSREGYIAIWMAIFTLLGLYLLGKIKFKFDSDLQHLGVFRLFLAIAS
                     FAFVFYLLPGLFGAPLKTISGLLPPQSSQSFDLKPNQVIVSQNTPSFNDENSLCEEPR
                     FADFLHLPHGLNGYFDYDQALRCAREKGKPLFIDFVGHTCANCKVMEAQVWSDPRVLE
                     RLRRDYIIVALYVDDRTELPESEWVTSARDGKVKKTIGKKNADFQATRFNVNGQPYYV
                     LLDNEEKLLTEPRGFDLNVEAFIQFLDKGVEEFKKRNQ"
BASE COUNT         2570 a         2011 c         1901 g         2261 t
ORIGIN      
        1 taattcatgg caattcggct caaaactcta ttacctttgc ggaaataaat gtttatgctg
       61 ccacagggtt gtaatccgaa atgtagaggg tgtagccaca gggattggtc catgaccgat
      121 agccttgagc agaaacaagc attcgtgaaa agtaaactaa ctgaatgggc cgatttggtt
      181 gaacccgtgc gctcagtacc ggagagtgag cgttggggct atcgcactaa aactacccta
      241 agcgcaaggt ttaatggaaa tatttgggag tttggaatgt ggcggcgcga cgagcttgtt
      301 ccaattccca actgccccgt acatgcaccg gagttgaata gagtactcgc cctgcttgcg
      361 gattcatttc cgcgggaaca ttcttttgca cttgcatacg tagtaatttc aggcgcacag
      421 gttgttctgg taatcaaatc gaagcaggaa ccaacattaa cctggcttag tgaagatgtt
      481 gcacaaggac ttgccagcat gggaattgaa gggatatggt accatatgaa tccttcaacg
      541 ggccgaagga tttttgagaa atccggatgg atgttaattt gggggaaaac ccgttcaact
      601 gactaccatg gctcacttta tggccccgct gcttttcagc agttgatacc cgaactgtac
      661 aacgaatcgc tggaggaggc ggaaaggttt ttaaacccta atgggaatag cgctcttatt
      721 gacttatact gcggaaccgg aaactcaatg cgacgatgga ttaaccgggg ggctaatgta
      781 attggggctg agcttggagg cgaggctgtg gagtgcgccc gtttgaatgc tccccaagct
      841 atggttttgc gcggtgcttg ccggcaaaga gttccacaaa ttgctgaatg gactgataaa
      901 aatcgctatc aagggaaaga aatattactt tacgtaaacc caccacgcac ggggcttgaa
      961 cctgaggttc tagaatggat ttcaaaacaa gggcttccca atcgaattgc ctacctatcg
     1021 tgtagcccag gcaccctctc aaagaacttg aattggctct gcacaaacgg ataccgatta
     1081 gtacgatgtg tgcccttcga tttcttccca caaacacacc atgttgaaac gcttgctttg
     1141 cttgagagag ttagcgctga tacgtattag catctaaact caaacagcta aaactttcct
     1201 ttaacgaggt tttatcttcg gccacaatta tgagcttatc gttaggctca ataactgttg
     1261 agcccgtagg cgaaatatac ctgtcgttgc gttgaattag cacgataagc gatttttcag
     1321 gaaaatttag gtcaactatt cgtttcccta caacgcagct atcgtcgggt atgtcaactt
     1381 ctaccatttc ggtgctaata ttttccctga gcataacatc aacaggggta atttgcttaa
     1441 cccgttgcgg taacaaaacc cttaaccact ttgctactcg aataagcgac gtaccctgta
     1501 ttaacaccga cgttatggtg ataaaaaata ctatattgaa tatctcttga gccttagcca
     1561 ttccggcaat caaggggtat gtggcaagta caataggcac agcaccacgc aatcccaccc
     1621 aagcaatgta ccaccgttca cgattcttca tcctataagg ggataggctt atgaaaacgc
     1681 tcaatggtcg tgcaataaac attaacccaa aggctataag caacccgttt cctatgtgcg
     1741 gaataatttc acccgcattc accagtaaac caagtgtaat gaaaaggatt atttgcataa
     1801 gccaagccaa accatcgaac attttcagaa tgatcttttt atgcattatg tggctatttc
     1861 ccagaagtat tgctgataag taaacggcta aaaacccatt ccctccaata aaatcggtta
     1921 tggaaaaggt taacaacatc agggtaattg ctaatacagg gtataaccct tcatattcga
     1981 gtttgatgtt gttaattatg tatttacttg catacccaaa gcccacaccc atgctaactc
     2041 caattattat ctggcttaga aagagtttta caacctccca gaatgatagc gatgggcttg
     2101 ttgcaagtcc aagaaaaatg atggtcaata gataagccat agggtcatta ctaccactct
     2161 caagctcaag ggttggcctc agattgccct ttagggctaa attttttgag cgaagtattg
     2221 agaaaacagc tgccgcatcg gttgacgata taattgagcc aattaaaaag ctctccgtaa
     2281 tactaaaatc agttactagc caggttataa gccccgagaa aagagccgtt aacaccaccc
     2341 ctagggtgga aagggttaca ccatgccata aaatcggctt aatgctaaac caattggttt
     2401 cgaacccccc tgcaaataga ataaagttta aggcaacaac cccaatgaac tgagcagtaa
     2461 tggcatcgtt aaaaacaact agtcctgaaa aagacgtgcc tgcaagcatt ccaacaaaca
     2521 aaaaaaagat gagcgttggg acacccaagc ggaacgatgt ttttccggct attaagctaa
     2581 taaatagcag caacgaacca ataagcaaaa agttttcggt ggtaaaattc atcagcctaa
     2641 tacaattcgt tcaaaatttt accttcgctt aaaaacgata ctccctgccc actggttaaa
     2701 cttaccatga tggtctcaac caggggctcg tttacagcag aatcggccat ccactccaga
     2761 atgaaatttg cgcccactcc gctcctgtcg ctttccggga caaagtagtt aactgattgc
     2821 atgggcatta ctgtatcaac tctcgattgc aaaaagtcct ttatcaggtt cccattggta
     2881 tcgaggtagt aaacgctaat aatgtaaatt ggattattaa aatcggtatt atgaacagcc
     2941 ataaacgcgc ttaacccata aaacgaattt ccctcggcaa atggtatgct ggagtaaacc
     3001 ggtaagtaga gcgtttgtgc cctaaccttt ttcacggggt tcttcacctt aatgtgaact
     3061 gggtcaaatt tagccagttt aacagtttgt ttttcatggt gtttacagcc aaaaagaaca
     3121 cctaaagcta gggcgataat taacgttgag tatctcataa ccaatatggt ttagtttgac
     3181 aattatttca tatcttgcaa tataaaaaat tagttctatt taagccaatt ttaccatgaa
     3241 aaggcatgca cttttacttg cattgattct tttggtcgct caagcttttg cgtgtaccac
     3301 agccattatc agcggtaagg ctacacccga tggacgaccg ctgctatgga agcaccgcga
     3361 tgccgatgac tttaacaaca aggttgtttt tgaggttggg ccacgtttta aatacttagc
     3421 gctcatcaac tcaagtgacc ctgagcgtca tgcctgggct ggcaccaaca acgccggatt
     3481 tgccataatg aactcggctt cgtacaacat caaacccaaa accgattcat caaaagttga
     3541 agaccttgag ggatacatca tgaaattggc attgggcagc tgcgccacca ttaccgattt
     3601 tgcccaaatg ctcgatacac tcccaaaacc gtggggcgta tcggcaaact ttggggtaat
     3661 cgatgccaag ggtggagcag gttactttga ggttaacaat tttagctacc gcttttacga
     3721 tgccaacagt acaaccgatg ctcccgaggg gtatttgatc cgttcaaact tttcagtttc
     3781 gggcgaaccc aataaaggag caggctacat tcgctttcat acggcatcaa acctgtttaa
     3841 ggaagctaag gcagctaacg ggctaacgca tgaattcctc ttaactaaag cctccgttag
     3901 cctaaggcag gatatcaccg gaattgattt atcaaaagag gtctcaaagg ccgaaaagat
     3961 ttaccctttc cgcgacttta tagtccgata ccactcatcg tcaaccgtag tggtgcaggg
     4021 tgttctgccc aaggagccta ccgattttgc caccatgtgg gtaaagttgg gctttcagcc
     4081 tgcatcggtt gccattcctc tgtgggttgg cgcaggtggc atactacccg atattattac
     4141 tgcccccgat gggctaaacg ccaagctatg cgatttttcc cttaaactaa aaaaggactg
     4201 cttcccgctt agctcatgga ccgaagggga aaactatgtg ctgctaagca aactcattaa
     4261 caaggagcaa accgggataa ttcagttaac caacattttc gataaggaaa ttattgcccg
     4321 caccaaaacg ctttacgata aatggacaaa atttgaattt aatcccattg aagcatacaa
     4381 tttctaccta acccttaaca gggatgttga aaagttctat aaaaaccatt tcgatttatg
     4441 accacacgta aacgactggt agttctcacg ggagcaggca ttagcgccga aagcggaatt
     4501 cgcactttcc gcgacatggg agggctttgg gaagagtatg atgttatgga ggtggcaagc
     4561 atcgatggat ggaagaaaaa cccacagctc gtactggaat tctacaacca acgaaggcga
     4621 caactgctaa ccgttgagcc caatgcagcc cacaaggtgc ttgccgaggt ccaaaacctc
     4681 ttcaacgtat ttgtaatcac ccagaatgtc gataacctgc atgagcgtgc cggcagtaaa
     4741 aatattcttc accttcacgg tgagttaacc aaggcaaggt caaccggcaa cccaagtaga
     4801 attatcgata ttggctacaa cgacattaaa tggggcgata ctgttgatgg ataccagcta
     4861 cgcccgcaca ttgtttggtt tggcgaagaa gttcccatga tgaacgaggc tgttaaactg
     4921 gttcaaacag ccgagatttt tgctgttatt ggcacctcgc tgaatgttta ccctgcagct
     4981 ggactgctcg atcatgcccc taagggttgt cccatatacc tagttgaccc aaacgatata
     5041 aatatctggc ggcgcgatgt tacggtgatt aaggagaaag catcggtggg ggttccaaaa
     5101 cttattgaga tgattaaaaa tgaatagggt actactcttg gggtttgttt tactgtgttt
     5161 ggggagcact caaatgcaag gccaaagctt taggggaggc ctactagccg gggtagccgc
     5221 cacgcaagtt gatggcgata cctacggcgg ttacgacaag gcaggaccca ttgcgggctt
     5281 ttgggtagaa cgcagtaagc tttccggatg gttttaccgc ttaagtttcc ggttcatcca
     5341 aaaaggtagt tatgctaaaa gtcgctctac cgatattccc gatttttacc gcatgcgtct
     5401 caactacttc gacctacctg tactggcagg atatcgtttt gtaaacggct tcaatgtcgt
     5461 tttggggcta tcgggtggct acctgagcaa agctaccgaa gagaattcgc ttgggccata
     5521 ccctcaggag gaagtatcaa cctttaagaa atttgaaatt gccggtattg ccggcatgga
     5581 gtataactac agctccaaat ggaggtttgg ccttaacctt tcctattcca tcctgcctgt
     5641 taggccttac aaggataata tatcgtaccg aatgaacaat gggcagtata atcgagttat
     5701 agagttcgtt tcaatttaca ggatacagtg atgttactca aacgcaaatt gttttttact
     5761 cttacgcttg ctctttttgt gttacttcat agcggcaaag cacaggaagg taggtacagt
     5821 atcgatataa aggagcggcc cggatttata atcccgcacc atagccacat agcctacttt
     5881 ctcaaggaaa atgtgaacgc cattcaactt aactttggca taaccaccga tggtagcaag
     5941 ttctggcata aggagttcaa ctaccccatt gtaggcttgg ggctatacta ttctggcttg
     6001 gggaacgata ctctttacgg taaacttacg ggtttataca ccttcataaa tcggccattt
     6061 ttggacaaca ccaaacggtt caatattagt aacacgctct cggtaggttt gagctatgcc
     6121 tcaaaatggc ataaccccat aagcaaccgc agcaacatgg tactaagtac acccctgaac
     6181 gttttcttcc agtacgagat tggatgcaat tacaggataa gcacaaacca taatgtctcg
     6241 gcaagtattg gacttaccca tgcatcgaac ggaagcgtaa aggaaccaaa cctgggattt
     6301 aacattttca ccaccgcact gggttaccgt tactcatttg gaaatccaaa tcctgtaaga
     6361 agtgaattgc ctgatgatat caccccattc tcatcatgga attttggact gttcgccagc
     6421 ataaaatcga tagatgcctt cagcggaaaa cagtatggca ttatcggcct atcggccgag
     6481 aaactatacc gctttgcacc cctatccatg gcagggcttg aactaacagc ctacaaggat
     6541 aactcgatac ccgacttagt aatggacaaa tcagaagatg cggttacact taacaaaaac
     6601 gacaacctgg ccttgacaat caatgcaaca tacctcatgg gcttcaatcg cattggtatt
     6661 gtttttcagc ccggtattta cctgcgaaac caatattatg gctttggcgc attttccaac
     6721 aaggtgggat tgcgcatcaa tatctatgac ggcttaatcc taggagttgc cattaaagct
     6781 cattggtttg cccaagccga ttttgttgaa tttggaatta aaaactcttt caataggcat
     6841 gaaaaataga atatacttat tgatactttt ttttggcatt ttctattcct gtagcctgat
     6901 aagcgattat acactgggcg atatggccga gcgccgcgta aacctacccg ccacctttga
     6961 aaaaattgat attaagggtt cgtttagcat caccctactc cccgacaccg ttggttttgc
     7021 ggtggttaaa tgccacgaaa aggttatgca cgatgttgag gttaaaattc agaacaacac
     7081 gctattccta agggaaaatg taaaaagccg atggctaaag gactacccca ttatcgacat
     7141 tgagttacac ctaaccgaaa tccccatgat tgaaatccac caaccctgta aatttactat
     7201 cccctacacc tttaaaagcc atgcatttta cttggtggac tggggcaact acgttgattg
     7261 cgatgtaaac attgaagttg attttcttaa gatcgacgtt tcaggcaaga gctttggaac
     7321 ataccgggca acaggccagg ccgagcatgc cacactgtat gctcgtggtg ctgctctttt
     7381 cgacctttcg caaacacagg taaaacgctg cactgcaaac caaggctcaa cggttaaaat
     7441 ggatatttgg gtaaccgata acctcatggc aaacattacc tcaaccggtg atattcactt
     7501 gcgcgggaac cctacaattg agctaaatag tcaaggaaaa ggggaattga ttagaacgcc
     7561 atagccactg cacaaatcaa aagaggccgc ctttggggca gcctcaatgt tcaaaaccca
     7621 atacttactg gttacgtttc ttaaactctt caaccccttt atcgaggaac tggataaacg
     7681 cctccacatt aaggtcaaat ccgcggggct cggtaagtaa tttttcctcg ttatcgagta
     7741 acacgtaata tggttgacca ttaacgttga accgggtagc ctgaaaatcg gcattctttt
     7801 tgccaatggt tttctttacc ttgccatcgc gagccgatgt tacccattcg ctttcgggta
     7861 attcggttct atcatccacg taaagggcaa caattatgta gtcgcggcgt agccgctcca
     7921 gcacccgtgg atcgctccaa acctgcgcct ccatcacctt acagttagca caggtatgcc
     7981 caacaaaatc gataaacaag ggctttccct tttcccgtgc gcaacgcaaa gcctggtcgt
     8041 aatcaaaata accatttaga ccatgaggta gatgaaggaa atcggcaaag cgaggctctt
     8101 cacacagcga gttttcatca ttaaaagagg gtgtattctg gctaacaata acctgattcg
     8161 gcttcaagtc aaaactttgg ctgctttgcg ggggtaataa accggaaatg gttttaagcg
     8221 gtgccccaaa taacccggga agcagataga aaacaaaggc aaagctggca atggccagga
     8281 ataaacggaa aactcccaag tgctgaaggt cggaatcgaa tttaaacttg attttaccca
     8341 aaaggtaaag ccccagcagt gtgaatatag ccatccagat ggcaatgtaa ccctcgcgac
     8401 taaacatctc aagctggtaa ccctgatcga tggtaagcag gaacttgagc gaaaaggcaa
     8461 gcacaatgaa tcccagaaaa acctttactg aattcatcca tccaccgctg cggggtagcg
     8521 agttaagcca cgacgggaaa attgcaaaca gggtgaacgg tatggagaaa gccagcgaaa
     8581 agccaaacat ccccacaatg ggttttgcac ccagccccga tgcagcctct actagcagcg
     8641 caccaacaat ggggcctacg cacgaaaggc ttacaataac tgttgttaaa gccaaaaaga
     8701 acgagccaat tagtcctccc ctatcggcct tgctatcggt ttt
//
LOCUS       DAOOWJ010000108         8682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_8087_length_8682_cov_7.958618, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000108 DAOOWJ010000000
VERSION     DAOOWJ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8087_length_8682_cov_7.958618"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLW22_09720"
     CDS             complement(<1..183)
                     /locus_tag="PLW22_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HOU75020.1"
                     /translation="MEVNRFESIKEGTILLIDKPYDWTSFDVVGKLRSLIKKYTGDKS
                     TKIGHAGTLDPLATGLL"
     gene            complement(187..963)
                     /locus_tag="PLW22_09725"
     CDS             complement(187..963)
                     /locus_tag="PLW22_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOU75021.1"
                     /translation="MTIFEAIIIGIIQGLTEFLPVSSSGHIELSKALLGVQLENNLEF
                     SIAVHVATVLSTLLVFRKEIIDLFSGFFSLKLNSQTHYVFKLIISAVPVGIIGVLFKD
                     QIETLFEGNLLVVGSMLLVTAILLALTRIVKPKNTKPVGYFDSVVIGLAQAVAVMPGL
                     SRSGATISTGLLLGKDRDEVARFSFLMVIIPVLGAAFLDIIKGDISNTITAPVVAGFV
                     AAFVSGFLACSFMLRIVRKGNLYWFALYCALVGIVALFLA"
     gene            complement(990..1259)
                     /locus_tag="PLW22_09730"
     CDS             complement(990..1259)
                     /locus_tag="PLW22_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOU75022.1"
                     /translation="MSAKNTPKKEIQEKENLFPLSWMNYKLMIIGFAIIVLGFILMAG
                     GGSTDPNVFNPDIFSFRRITLAPVVVLFGFAFVGYAIMKREKKDS"
     gene            complement(1279..2157)
                     /locus_tag="PLW22_09735"
     CDS             complement(1279..2157)
                     /locus_tag="PLW22_09735"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOU75023.1"
                     /translation="MANSVNRATKGRLRSSYISSIISISLVLFTLGLLGVLVISAKKL
                     SAYVKENIGFDIYLNEGVTDADALYLKKQLDASIYIKSTQYFTQQDAAKMMANELGED
                     FIAYLGYNPLNAYIEARLKADYANNDSIARIEGWLKRFPQVKEVDYQRSLINLVNDNV
                     SRISLVILTFAGLMLFISLVLINNTIRLSVYSKRFIINTMKLVGASWGFIRRPFLLKS
                     ILHALYASFIAIALLIGLLYAINKEVADLLAILDPPSIVLVFAAITAIGIVINFLATL
                     MAVNKFLRSSTDDLYY"
     gene            complement(2158..4572)
                     /locus_tag="PLW22_09740"
     CDS             complement(2158..4572)
                     /locus_tag="PLW22_09740"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOU75024.1"
                     /translation="MNSKFSKIKPHIVALLLFMAIPLAYFYPQLQGKRLAQHDIAMWK
                     GSAKEILDYKAKTGEESLWTNSMFGGMPAYLISANYKGNLLKHVDSFLNVVGRPASYI
                     FLTMLGFYILLLVFGVNPWLAIVGSIAYGLSTYFLIVIGAGHNAKIHAIAYVAPTIAG
                     MILALRGQLFAGLAVFGLAFGLNLNAGHPQITYYGALVMASVYLYYFVEAFKAKALRM
                     FTKATGILLLAAVLAFGANFTYLWFTNDYGKDTIRGKSELTHDRDNRTSGLDKDYATQ
                     WSYGIAETFNLLIPNLMGGSSTMDVGEKSETFRFLRQNGVPYGQASSIAQNLPTYWGP
                     QPSTSGPVYLGAIVVFLFVFGMFILKGRFKWFLFGITVLAIMLSWGKNFNFLTDLFLN
                     YFPFYNKFRTVSMILVIVELTIPLLGILALKEIVDGKVTKDDFWKSFKWSVGLVGGIC
                     LLFLLLGKGMLSFSGSIDNQLGWPKEIVEVMRKDRQALMWNDAFRSLFFVLAGAVTIA
                     LYYMKKLKPTLFFALLGLLITVDLWVVNKRFMDNKNFELPQQVEAPFTPTDADKQILA
                     DNDPNFRVFNLTVSPFNDASTSYFHKSIGGYHGAKLRRYQDLIDFHLSKGNIAVFNML
                     NTKYIIQPGKEGPVAVYNPDALGHAWFVDSVKVVNIADEEIEALNGFNPKSIAVVDKR
                     FEQYLRGFEQGKSGQGKIELTEYRPNRLKYRSDSPVSQLAVFSEIYYPKGWQAYLDGT
                     PADHFRVNYVLRAMQIPAGNHEIEFVFDPPMWKKGMYIDLASSLLLILTFVGWVSFSL
                     YKTFKE"
     gene            complement(4589..6139)
                     /locus_tag="PLW22_09745"
     CDS             complement(4589..6139)
                     /locus_tag="PLW22_09745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOU75025.1"
                     /translation="MTQEELFKKLIAHSKEYGFVFPSSEIYDGLSAVYDYGQNGVELK
                     NNIRRYWWEAMTRLNENIVGIDAAIFMHPTTWVASGHVGAFNDPLIDNKDSKKRYRAD
                     VLIEEWIEKQREKIDKEIEKARKRFGDAFNEELFRQTNPRVVELSESITTVNHRMVEA
                     LEQNNLAEVKKIIEDCEIVCPISGTRNWTEVRQFNLMFETKLGSVSDEANTIYLRPET
                     AQGIFVNYINVQKTGRMKIPFGIAQVGKAFRNEIVARQFIFRMREFEQMEMQYFVRPG
                     EEMKWYEFWMEKRMRWHRAMGFGDNKYRFHKHEKLAHYANAASDIEFEFPFGFKEVEG
                     IHSRTDFDLANHQKHSGKKLQYFDPDINQSYIPYVIETSIGLDRTFLLVLSAAYAEEK
                     VAKEDGTSDERVVLRIPPALAPVKLAVFPLVKKDGLPEMARKIMDDLKFDFACQYEEK
                     DTIGKRYRRHDAIGTPFCITVDHQTLDDETVTIRYRDTMEQERVPASRLREIMNDKVS
                     LTNLLRQI"
     gene            6275..>7378
                     /locus_tag="PLW22_09750"
     CDS             6275..>7378
                     /locus_tag="PLW22_09750"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HOU75026.1"
                     /translation="MVHLKPFITTITKYLVLLLAFTSAVHAQYLANFTNNKSTKIDEV
                     KPLSQIPEYKIKAALILNIASNIEWPNERNIKEFGILIVDSDTLIYNEIRSVETSYKL
                     KGKPIRVNLSSPPVSKPTEYSIIYLSHSYSPLVNSIYQQIVGKGVLIVTDEGTERLLT
                     MVNLFLNQEKQSVSFEINRHTLEENSFTLSPKLVALGGSFIDIKELYLKTYHQLIEEN
                     KKLQQLQTDLTRYQQEKTEYESEIRTLNERVNRLNQNTAVLEKQYSNASVSLKQKDSL
                     LLIATKELAEKVAESQKLQTAINKQVALINSSRSQLDSLNKQIVQTQKVLDQKQKEIE
                     EQDIVISEKESIILKQQRRFFIMLIALVGISFTL"
     assembly_gap    7379..7478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7479..8573
                     /locus_tag="PLW22_09755"
     CDS             <7479..8573
                     /locus_tag="PLW22_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU75027.1"
                     /translation="SEYESIRNTKEGRKLFVVLKWIVLPGYSHDYSRVLLSITDITKL
                     KEYEKELTQHRDHLEEIVQERTQQIIQLNKMLQESNEELLLKTEELEQTIHLLEDAQK
                     QLVQQEKMASLGMLTAGIAHEINNPINFISGSQQAIESLLDELWNSLNIYREKALKHS
                     PKNITYELETNNSIQVEELYSSIKLMLRNIEIGIERTTGIIRSLKAFVRSDSKEYVPV
                     SIPETIADVLNMLRGNYKDRIEIIQHYDQDLPKVKCYPNQLHQVVMNLLANAIDAIPG
                     EGKIYISGQYSSSDNNVTIRIKDTGTGIPSDIQEKIFDPFFTTKDAGKGTGLGLYITY
                     NIIKSLNGDIKVNSAPNQGAEFTVTLTACE"
BASE COUNT         2669 a         1910 c         1723 g         2280 t
ORIGIN      
        1 caataggccc gtagccaatg ggtcaagggt tcctgcatgg ccaattttag ttgatttgtc
       61 gccagtgtat tttttaatca acgatcgtaa cttccccaca acatcgaacg aagtccaatc
      121 gtatggctta tcgataagta atatggtacc ctccttaatg ctctcgaaac ggtttacctc
      181 catgggctag gctaaaaaca gtgcaactat accaaccagc gcacagtaaa gtgcaaacca
      241 gtaaagattc cctttccgaa caatgcgaag catgaatgag caggccaaaa agcccgatac
      301 aaatgcagca acaaatcctg caactacggg cgcagtaatg gtgtttgaaa tatcaccttt
      361 gataatatcg agaaaagcag ctccaagtac gggaataata accataagga acgaaaagcg
      421 agctacctca tcgcggtcct tgccaagcaa aagtccggta gagatggtgg cacccgaacg
      481 acttaagccg ggcataacgg caacagcctg ggctagacct atgacaacag agtcgaaata
      541 tcctactggt tttgtgtttt tgggttttac tattcgggtt aaagcaagaa gaatggctgt
      601 aacaagcaac atggaaccca ctaccaataa atttccctca aaaagagtct caatttggtc
      661 cttgaataaa acacctataa taccaacagg aaccgctgat attataagct taaatacgta
      721 atgggtttgg ctgttcagct ttaagctgaa gaatcccgaa aataaatcga ttatctcctt
      781 acggaaaaca agtagggtgc ttaaaaccgt agccacatga actgcaatgc taaactccag
      841 gttgttttcg agttgaactc caagcagagc ctttgatagt tcaatgtgtc cgctactact
      901 tacaggtaag aactcggtta aaccctgtat aatgccaata attatggcct caaaaatggt
      961 catttcaaat actttttaat gaaaaaaaat tagctatcct ttttctcgcg tttcattatg
     1021 gcgtaaccca caaatgcgaa cccaaacagt acaacaacgg gggcaagcgt aatacgtcgg
     1081 aaactgaaaa tgtcggggtt gaacacattg gggtcagttg atccaccgcc tgccatgaga
     1141 atgaaaccca gaactattat ggcaaagcca attatcatca gcttatagtt catccagctt
     1201 aagggaaaaa gattctcttt ttcctgtatc tcctttttgg gggtattttt tgcagacata
     1261 actgttaatt ttatcgggtt aatagtaaag atcatcggtg cttgagcgta aaaatttatt
     1321 cactgccatc agggttgcta aaaagttaat aactattcca attgcagtaa ttgctgcaaa
     1381 tacaagcaca atgcttggcg gatcgaggat ggcaagcaaa tcggcaacct ccttgtttat
     1441 agcatacagc aatcctatta acaaagcaat ggctataaag gaagcgtata gggcatgtaa
     1501 aatgcttttc agcaggaaag gtcgtcgtat aaaaccccag cttgccccaa ctaatttcat
     1561 agtgttaatg ataaatcgct tggagtaaac ggacaaccta atagtattgt taatgagcac
     1621 aagggaaatg aaaagcatca gtcctgcaaa tgtcagtatc accaagctaa ttctactaac
     1681 attatcgtta accagattga tgagtgagcg ctggtagtca acctccttta cctgggggaa
     1741 acgctttaac cacccctcaa tgcgtgcaat gctatcgtta ttggcgtaat cggctttaag
     1801 ccttgcctca atgtatgcat ttaatgggtt gtatcccagg taggcaataa aatcctcgcc
     1861 aagctcatta gccatcattt tagcggcatc ttgttgtgtg aagtactggg ttgattttat
     1921 gtagatacta gcatcgagct gctttttcag gtaaagggca tcggcatcgg taactccctc
     1981 gttcaggtag atatcgaacc caatgttttc cttaacgtac gctgaaagtt tctttgcgct
     2041 aataaccaaa accccgagca aacccaaggt gaaaagcact aaggagatgc ttattattga
     2101 tgagatatag gaactcctca acctcccttt agttgccctg tttactgaat tagccattta
     2161 ctccttaaat gttttataca aactaaagct tacccaacca acaaaggtaa gtattaatag
     2221 caacgatgaa gccaaatcga tatacattcc ttttttccac ataggcgggt caaatacaaa
     2281 ctcaatttca tggttaccag caggtatttg catggctctt aacacgtaat ttaccctgaa
     2341 atgatcggct ggtgtgccat cgaggtacgc ttgccaacct ttgggatagt aaatctcaga
     2401 gaaaacagct agctgagaaa ctggcgaatc gctcctatac ttcagcctat taggacggta
     2461 ttcagtaagt tcaatcttac cctgacccga tttaccctgc tcaaaacctc taaggtattg
     2521 ctcaaatcgt ttatcgacta ctgctattga ttttgggttg aagccattca gggcctcaat
     2581 ctcctcatcg gcaatattaa caactttaac tgagtccaca aaccatgcat ggccaagtgc
     2641 atcggggtta taaactgcaa cgggaccttc cttgccgggt tgtataatat actttgtgtt
     2701 taacatgtta aacacagcaa tattaccctt ggatagatga aaatcaatta aatcctgata
     2761 gcgacgcagc ttggctccat ggtatccgcc aattgattta tggaaatagg atgtggatgc
     2821 atcgttgaat gggctaacgg taaggttaaa cacacggaaa tttggatcgt tatcggctag
     2881 tatctgctta tcggcatcgg ttggggtaaa gggggcttca acctgctggg gtaactcaaa
     2941 gttcttgtta tccataaagc gcttgtttac tacccacaaa tccacagtaa tgagcaggcc
     3001 cagcaaggca aagaatagtg ttggcttcaa ctttttcatg tagtaaagcg caatggtaac
     3061 tgcaccggca agtacaaaga atagtgagcg gaaagcatcg ttccacatca gcgcttgcct
     3121 atccttgcgc attacctcaa caatctcctt gggccaaccc agctgattgt caattgaacc
     3181 gctaaaggat aacattccct tgccaagcag caggaacagc aggcaaattc ctccaaccaa
     3241 tcccactgac catttaaatg atttccaaaa atcatccttt gtaaccttgc catcaactat
     3301 ttccttaagg gcaagtattc caagcaaagg aatggttaac tcaactataa ccagtatcat
     3361 cgataccgtt cggaacttgt tgtaaaaggg gaagtaattc aggaataaat cggtaagaaa
     3421 attaaaattt ttaccccacg agagcattat tgccagaaca gtaatcccaa acaaaaacca
     3481 cttaaatcta cctttaagga taaacatccc gaatacaaaa agaaaaacta caattgcacc
     3541 aaggtaaaca ggaccagagg ttgatggctg tgggccccag tatgtgggta ggttttgtgc
     3601 tatggaacta gcttgaccat agggaacacc attctgcctg agaaaacgga atgtttccga
     3661 tttttcgcca acatccatgg tgcttgaacc gcccatcaaa ttagggataa gcaggttaaa
     3721 agtttcagct attccgtaac tccactgggt tgcatagtcc ttgtccagcc ccgaagttct
     3781 attatcccta tcatgggtta actcagattt gcctctgatt gtatccttgc catagtcgtt
     3841 agtgaaccat aggtaggtaa agttagcgcc aaaagccaga actgctgcca gaagcaaaat
     3901 cccggtagcc ttggtaaaca tccttaaggc ttttgcctta aatgcctcaa caaaatagta
     3961 gagatagaca gaggccatta caagtgcacc gtagtaggtg atttgcgggt gccctgcatt
     4021 gagattgagt ccaaaagcta gtccaaaaac tgccaaacca gcaaaaagct gtccacgtaa
     4081 agccaaaatc attccagcaa tggttggggc aacataagct atggcatgaa tcttagcgtt
     4141 atgccctgct ccaataacta tgagaaaata ggttgatagg ccataggcaa ttgaacccac
     4201 aatggcaagc caagggttta ccccaaaaac aagaagtaga atataaaatc ctaacatggt
     4261 taggaagata tagctggcag gccgtcccac cacatttagg aagctatcga catgctttag
     4321 caggtttccc ttgtagttag ccgaaatgag atatgccggc attcctccaa acatactgtt
     4381 tgtccaaagc gactcctcgc ctgtttttgc cttgtaatcc aaaatttctt tcgctgagcc
     4441 tttccacata gcaatatcgt gctgagctag ccttttaccc tgtagctgtg ggtagaaata
     4501 agctaacgga atggccatga aaagcagcaa ggctactata tggggcttta tcttactaaa
     4561 ttttgaattc atgccggcaa gtgtttattt atatttgacg tagtaggtta gttagcgaaa
     4621 ccttatcgtt cattatctcg cgcaggcgcg atgcgggaac acgctcttgc tccatggtat
     4681 cgcggtagcg aatggttacc gtttcatcgt cgagcgtttg gtggtcgacg gtgatacaga
     4741 atggagtgcc tattgcatcg tggcggcggt aacgcttgcc aatagtatcc ttttcctcgt
     4801 actggcatgc aaaatcgaat tttaggtcgt ccattatttt gcgcgccatc tcgggtaatc
     4861 catccttttt aaccagtggg aatacagcaa gcttaacagg agccagggct ggcggtatgc
     4921 gaagcactac gcgctcatcg ctggtaccat cctccttagc cactttttcc tcagcgtaag
     4981 cagccgaaag aacaagcagg aaagtacggt ctaagccaat tgatgtttca attacgtaag
     5041 gaatatagct ttggttgatg tcgggatcaa agtactgcag ctttttaccc gagtgctttt
     5101 ggtggttagc caaatcgaaa tcggtgcggg aatgaatccc ctccacctct ttaaacccga
     5161 atgggaattc aaactcgata tcggaagcgg cattggcata atgggcaagt ttctcgtgct
     5221 tatggaagcg gtacttgttg tcgccaaacc ccatggcacg atgccaacgc atgcgtttct
     5281 ccatccagaa ctcataccat ttcatttcct cgccagggcg aacaaaatac tgcatttcca
     5341 tttgttcaaa ctcacgcatg cggaatatga actgccgggc aacaatctcg ttgcgaaaag
     5401 cctttcccac ctgagcaatt ccaaaaggaa ttttcatacg cccggttttc tgaacgttga
     5461 tgtagttaac aaaaataccc tgagcggttt cgggcctaag gtagattgtg tttgcctcat
     5521 cgctaaccga gcctagcttg gtttcaaaca tcaggttaaa ctggcgtacc tcagtccagt
     5581 tgcgggtacc ggaaataggg caaactatct cgcaatcctc aattatcttt ttaacctcag
     5641 ccaggttgtt ctgttccaga gcctcaacca tcctatggtt aacggtggta atactttcgg
     5701 agagttcaac tacccttggg ttggtttggc ggaaaagttc ttcgttaaaa gcatcgccaa
     5761 aacgcttgcg ggctttttca atctctttat ctatcttttc gcgctgtttt tctatccatt
     5821 cctcaatgag gacatcggca cggtaacgtt tcttactatc cttgttgtcg ataagagggt
     5881 cattgaaagc gcccacatgc cccgatgcca cccaggttgt agggtgcatg aagatagccg
     5941 catcaatacc aacaatgttt tcgttcaggc gtgtcatagc ctcccaccag taacggcgta
     6001 tgttattctt cagctcaaca ccgttctgcc cgtaatcgta aacagcgcta agcccgtcgt
     6061 aaatctcgct cgatgggaat acaaatccat actccttgct atgtgcaatc agctttttga
     6121 atagttcctc ctgtgtcata aatgctttaa atgaatttcc agtaactcac aaaaatagtt
     6181 caatttcttt acatccaaaa aaaatgagtt ttggcaaata tgttttgact ttgtaaatgg
     6241 catttcatat attaccaaaa attttgatac tgcaatggtt cacttaaagc ctttcataac
     6301 aaccattaca aaatatcttg tactactttt agcattcact agcgcggttc atgcacagta
     6361 tcttgcaaat ttcacaaata acaagtcaac aaaaattgat gaggtaaaac ctttatcaca
     6421 aatccctgaa tataaaatta aagcagccct tatccttaat attgccagta acattgaatg
     6481 gccaaacgaa aggaacatta aagaattcgg aatactaatt gttgacagcg atacacttat
     6541 atacaatgaa atcagaagtg ttgaaaccag ctacaagctt aaaggtaaac ccataagagt
     6601 aaatctttcg tcgcctccag taagtaaacc aacagagtat tcaattattt acctaagcca
     6661 cagctacagc cctttagtta actcaattta ccagcaaata gttggtaaag gtgtgctcat
     6721 tgttaccgat gaaggaactg aacggctact taccatggta aacctttttc tcaaccagga
     6781 aaaacaaagt gtgtcattcg agattaaccg gcataccctt gaagagaata gttttacgct
     6841 cagcccaaaa cttgttgccc ttggtgggag ctttattgat attaaagagc tttacctaaa
     6901 aacctaccac cagctaattg aagagaataa aaaactgcag caactacaaa ccgacctaac
     6961 ccgctaccag caagaaaaaa cagaatacga aagcgagatc agaacgctaa acgaaagggt
     7021 taatagatta aatcaaaata ctgcagttct tgagaagcag tattccaatg caagtgtttc
     7081 acttaagcaa aaagatagcc tgctcctaat tgcaacaaag gagcttgccg aaaaagttgc
     7141 agagagtcaa aaactacaga ctgccattaa taaacaggtg gcattgataa actcctcgcg
     7201 tagccaactg gactcactga acaaacaaat tgttcaaacc caaaaagttt tagaccagaa
     7261 acaaaaggag attgaggagc aggacatagt tatatcggaa aaggagagta tcattcttaa
     7321 gcagcaaaga cgattcttta ttatgcttat tgcccttgta ggaatttcat ttactctgnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag cgaatacgaa agtataagga
     7501 atacaaagga aggcaggaag ctttttgtgg ttttaaaatg gatagttcta cccggatact
     7561 cccacgatta ctccagagtg ttgctatcaa ttactgacat aacaaagctc aaggaatacg
     7621 agaaagagtt gacccaacac cgtgatcatc tggaggaaat tgttcaagaa cgcacccaac
     7681 agattatcca gctgaacaaa atgctccagg agtcaaatga agaactactg cttaaaaccg
     7741 aggagttaga acaaaccatc catttactgg aagatgccca aaaacaactt gtacagcagg
     7801 aaaaaatggc ctcacttggt atgctcaccg ctggtattgc gcatgaaatc aacaacccca
     7861 ttaacttcat tagcggtagc caacaggcta ttgaatcgct gctcgatgag ctttggaata
     7921 gtctgaacat ttaccgcgaa aaggccctaa agcactcgcc caaaaacatt acctatgaac
     7981 ttgaaaccaa taatagcatt caggttgagg aactttacag ctccataaag ctaatgcttc
     8041 ggaatattga aattggtatt gagagaacta ctggcattat caggagccta aaagccttcg
     8101 ttcggtccga tagtaaagag tacgttccgg tttcaatacc cgaaaccatt gccgatgtgc
     8161 tcaacatgct gcggggtaac tataaggata ggattgagat tattcagcac tacgaccagg
     8221 accttcccaa agtcaaatgt taccctaacc agctacatca ggttgtaatg aatttgttag
     8281 ccaatgccat tgatgccata ccgggcgaag gaaaaattta cataagcggt cagtactcca
     8341 gctccgataa caacgttacc attcgtataa aagacactgg cactggaatc ccaagcgata
     8401 tccaggaaaa gatcttcgac ccgttcttca ccactaagga tgctggtaaa ggaactggtt
     8461 tggggcttta catcacctac aatattataa agtcattaaa tggcgatata aaagtaaact
     8521 cagcaccaaa tcaaggggct gagtttacag taacactaac agcttgcgag taaatatagt
     8581 ttctcaacta ttttcaaacc ttttaaaagc tgactcaatt atctcacgaa gctcatcctt
     8641 cttccagggc ttcatgatgt agcggtacac caactcctca tt
//
LOCUS       DAOOWJ010000109         8614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_8154_length_8614_cov_7.185886, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000109 DAOOWJ010000000
VERSION     DAOOWJ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8154_length_8614_cov_7.185886"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLW22_09760"
     CDS             <1..753
                     /locus_tag="PLW22_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOU75028.1"
                     /translation="ASATNGIICALDVIRTGKANVVITGGSEASVNEIGVAGFNALQA
                     LSTNNEEYKTASRPFDATRDGFVMGEGAGCLILEEYEHAKARGAKIYCELVGGGMSAD
                     AYHLTAPHPEGLGARKVMENALRDAGMKPEDIDYINVHGTATPLGDVAETKAIIQVFG
                     EHAYKLNISSTKSMTGHLLGAAGAIEALAAVMAVYKDVIPPTINLKEVDPEIDSKLNL
                     TPNVAQKRTVRAALSNTFGFGGHNASIIVKKM"
     gene            763..1557
                     /gene="rnc"
                     /locus_tag="PLW22_09765"
     CDS             763..1557
                     /gene="rnc"
                     /locus_tag="PLW22_09765"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOU75029.1"
                     /translation="MLKFLSKPIRRLRLTAADKEFYQNLKKILGFAPDNLDVYKLALV
                     HRSASLIISPGQKVDNERLEYLGDAILDAIVADYLFAEFPNSDEGFLTKMRAKIVSRN
                     NMNQLAISIGLPDVVKVNNGALNHQKYIYGNALEAIVGAMFIDKGFKFTSDVVVNNFL
                     RRHINLEELESIEYDYKSRILEIAQKLHIKVAFDTHDYGDNKQKPQFEAILLWNGKEI
                     GRGLGQSKKEAEQQASMQGIDLAEGELQSEKQKEDTPNEDPKNELI"
     gene            complement(1593..2171)
                     /locus_tag="PLW22_09770"
     CDS             complement(1593..2171)
                     /locus_tag="PLW22_09770"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOU75030.1"
                     /translation="MNQADIKNIINESVNTLRSGGIILYPTDTIWGLGCDATNQEAVT
                     RLMALKKRNDSRGFIILVDLPARIPSYVVDIPEMAWDLIELSDKPLTLVFDKGKNLAP
                     SVYGEKGTIAIRVTRSKFCHQLISRFGRPIVSTSANICGSPFPENFEAIPQEIVSGVD
                     YVVPKIIDTESTGKPSSIIALGKGNQIRIIRE"
     gene            complement(2184..3230)
                     /locus_tag="PLW22_09775"
     CDS             complement(2184..3230)
                     /locus_tag="PLW22_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOU75031.1"
                     /translation="MKAILFCINPYSFGILKPLNDELIARGNEVLWYVPDRIADKFPF
                     KNESIVTNSIKTLYDFKSDAIFVPGNEVPHYLSGVKVQVFHGLAGEKKGHFRIRNYFD
                     LYLTQGPYFTSRFEDLAKKHKDFKVKETGWCKLDTLFGSKNSFNNEREEILKNSNKKR
                     IVLYAPTFSPSLTCAKHAKDKIFEIAETEDVFVIIKFHDLMDKAIAEEYIALAANRPN
                     VTVSEERNILKFLIISDIMISDTSSVVYEFTLLDKPVVTVNSKSHNICWRDINDVEHL
                     RKALIEELEQDNFRENRKLIIEQYHPYSDGKSSARMIEAVEDYIRNFGVPEKRKLNIY
                     RKYLMNKMFGKEPK"
     gene            complement(3246..3935)
                     /locus_tag="PLW22_09780"
     CDS             complement(3246..3935)
                     /locus_tag="PLW22_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOU75032.1"
                     /translation="MKIVILAAGIGSRLGNPLPKPLTVLKSGKSIMQQQIDNLTTYFD
                     IDDINVVVGFKKDVIMESFPNLTYIYNQNFDVTNTSKSLLKALNKYKNTSVLWLNGDV
                     VFDVEILKMLLPLIQQNQSFVCVNNSAVGEEEVKYNLDSNNFIKEISKKVVDAKGEAV
                     GINFISSNDIKTFIKYLEECNDNDYFERGLELAIEKEGIKVLPVDISSHKCLEIDFKE
                     DLENANRLFNK"
     gene            complement(4040..4822)
                     /locus_tag="PLW22_09785"
     CDS             complement(4040..4822)
                     /locus_tag="PLW22_09785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU75033.1"
                     /translation="MPKISAVIITLNEERNIERCLNSVLKVADEIVVVDSFSTDSTRS
                     ICEKFGARFIQNTFTGYRDQKNFAIEQASFDCILSLDADEALSPELEKSILEVKNNWT
                     CDAYKFSRLNNYCGQWIYHSNWYPDRKIRLFDRRKGRWGGLNIHETVKMQPGSKICTL
                     PGDLLHWSLHTYEEHIDKANRFSTISANEYYRMGVKSSIFKIIFNSSWRFFKAYFLKL
                     GFLDGYNGFVISSLSAYTSFLKYIKLHQLYINDRKKRNNKHT"
     gene            4939..5553
                     /locus_tag="PLW22_09790"
     CDS             4939..5553
                     /locus_tag="PLW22_09790"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HOU75034.1"
                     /translation="MGNKNIPNLTKGNLARARKMLFDVVEVLDNANIVYHLEGGTLLG
                     IVRDGDLLPWDHDVDLSIMITDTPKFLKIKKELYRKGYKVTSKKSKHDIHPFRKGDYK
                     IFKIKKMIPSVVKWLFPRYKKNYVVLDIFIKVNDGNYCYWQAMNKLMRVSNKYYASYE
                     TVNYRGASLKVPNDYKDYLTQKYGDWSVSVKEWVCGEDEKTIVS"
     gene            complement(5727..7943)
                     /locus_tag="PLW22_09795"
     CDS             complement(5727..7943)
                     /locus_tag="PLW22_09795"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOU75035.1"
                     /translation="MIIDHLFWIVPIASVIALAFAYHFFRQMMREDEGTDLMKKIAQH
                     VRTGAMSYLKQQYKVVVIVFIVLMLLFVFLAFGLGVQNKWTPFAFLTGGFFSGLAGFF
                     GMKTATYASARTANAAKNSLNHGLRVAFRSGAVMGLVVVGLAVLDISLWYLILNYFID
                     EPDSSHKLIIITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLYESYAGSILATAALGATAFAATGVDMQYKAILA
                     PMLIASVGIVLSILGIYMVRTKEGATMRDLLRSLGVGVNVSSALIAILTFVILYALGL
                     QNWMGISFSVVTGLLAGIIIGLSTEYFTSHSYKPTRKISESAQTGPATVIISGVGLGM
                     VSTAIPVITIAIAILIAFLSAINFDVKHMLDAQNIQLGLYGIGIAAVGMLSTLGITLA
                     TDAYGPIADNAGGNAEMSHLDPEVRKRTDALDALGNTTAATGKGFAIGSAALTALALL
                     ASYIEEIKIGLIRIGENVITLANGDTIDTAKATIVDFMNYYQVNLMNPVVLVGVFIGA
                     MMAFLFSGLTMNAVGRAAQKMVEEVRRQFREIKGIMEGEAEPDYARCVAISTRGAQIE
                     MILPSLLAIILPIVTGLVLGVAGVLGLLAGGLAAGFVLAIFMANSGGAWDNAKKYIEE
                     GNFGGKGSNNHKAAVVGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGLTAAFSLL"
BASE COUNT         2569 a         1823 c         1768 g         2454 t
ORIGIN      
        1 gcatcggcaa ccaacggtat tatttgtgcc ctcgatgtaa tccgaaccgg taaagcaaac
       61 gttgttataa ccggaggttc tgaggcctca gttaacgaga ttggcgtggc tggattcaat
      121 gcgcttcagg cactatcaac taacaacgag gaatacaaaa ccgcctcgcg tccgttcgat
      181 gctacccgcg atggttttgt gatgggcgaa ggtgcaggtt gccttatcct ggaggagtat
      241 gagcatgcta aagcccgtgg ggctaaaatt tactgtgagc tggtaggcgg cggcatgtcg
      301 gccgatgcat accacctgac agcacctcac cccgaggggc ttggtgcccg taaggttatg
      361 gaaaatgcct tacgcgatgc cggcatgaaa cccgaggata tcgattacat taacgttcac
      421 ggtacagcca ctccacttgg cgatgttgct gaaacaaaag ccattatcca agtttttggt
      481 gagcatgcct acaagctaaa tatcagctca acaaaatcga tgaccggtca cctgcttggt
      541 gctgctggtg ccattgaggc tcttgctgct gttatggccg tttataaaga tgtaatacca
      601 ccaaccatca acctaaaaga ggttgatcct gaaattgaca gcaagcttaa cctaacgcca
      661 aatgttgctc aaaagcgaac tgttcgtgct gctttaagta acacctttgg ttttggtgga
      721 cacaacgcat ccatcatcgt aaaaaagatg tagtttttca tcgtgttaaa attcttaagt
      781 aaacccatac gtcggttacg cttaacggcc gccgataaag aattttatca gaatcttaaa
      841 aaaattttgg ggtttgcccc cgataatcta gatgtttaca agctggcgct ggttcacagg
      901 tcagcttcgc ttattatatc gcctggtcaa aaggttgata atgaaaggct ggaatacctt
      961 ggcgatgcca tactcgatgc cattgtggcc gactacctat ttgctgaatt ccccaatagc
     1021 gacgagggtt tccttactaa aatgcgggca aagattgtta gccgcaacaa tatgaaccag
     1081 cttgcaatca gtattgggct acctgacgtg gtcaaggtaa ataatggtgc ccttaaccac
     1141 caaaagtata tttacggtaa tgccctggaa gccatagttg gcgccatgtt tatcgataag
     1201 ggatttaagt ttaccagcga tgtggtggta aacaacttcc tacggcgcca tatcaacctt
     1261 gaagagctgg aaagcattga atacgattac aagagccgaa ttctggaaat tgcccaaaag
     1321 cttcacatta aggttgcctt tgatacccac gattacggcg ataataagca aaaaccacag
     1381 tttgaggcaa tcctactctg gaacggaaaa gagattggcc gtggcctagg ccaatcgaaa
     1441 aaggaagcgg aacagcaagc ctcaatgcaa ggaattgact tagccgaggg cgaattacaa
     1501 agcgaaaaac aaaaggaaga tacaccgaac gaagatccca aaaacgagct aatttaaacc
     1561 ctttggccac accgctcagc gcaaggtttt gtttactccc taataattct aatctggtta
     1621 cctttcccta aggcaataat cgatgaaggt tttccggttg attctgtatc gataatctta
     1681 ggaaccacat agtcaacccc cgatactatt tcctggggaa tagcttcgaa attctctggg
     1741 aatggagagc cgcaaatatt tgcagaggtt gacactattg gacggccaaa ccttgaaata
     1801 agttgatggc agaatttgct ccgggtaaca cggatggcta ttgtcccttt ttcaccgtaa
     1861 accgatgggg caagattctt tcccttatca aacacaaggg taagcggttt atcggataat
     1921 tcaattagat cccaagccat ttcggggata tccaccacgt atgaagggat tcgggctggc
     1981 aaatcaacca gaataataaa acccctacta tcgttacgtt tttttaacgc catgagtctg
     2041 gtcacagcct cctggttggt ggcatcacag ccaagccccc atatggtatc ggtggggtag
     2101 aggataattc cgcccgaccg aagggtatta accgattcgt tgattatgtt tttaatatca
     2161 gcctgattca attgcaatgg tttctacttg ggctccttcc caaacatttt attcataagg
     2221 tatttccggt agatgttaag ctttcgcttt tcgggtaccc caaaatttct tatataatct
     2281 tcaaccgctt caatcatcct tgccgatgat ttcccatcgg agtaagggtg atactgttca
     2341 atgataagtt ttcggttttc cctaaaatta tcttgctcaa gttcttcaat aagggccttt
     2401 ctaagatgtt caacatcatt aatatccctc cagcaaatgt tgtgcgattt ggagttgaca
     2461 gtaacaaccg gtttatcaag aagagtgaat tcgtatacca ccgaggaggt atcgctaatc
     2521 ataatatcgg aaatgatcaa aaactttaaa atgttccttt cctctgaaac tgtaacattt
     2581 ggtcgattgg ctgctaatgc aatatattcc tctgcaatag ccttatccat taggtcgtgg
     2641 aacttgatta tcacgaaaac atcctcagtt tcggcaatct cgaaaatttt atcttttgca
     2701 tgtttggcgc aagttaacga gggggaaaag gttggagcat aaagcactat tcttttttta
     2761 ttggaattct ttaggatttc ctctcgctcg ttgttgaagc tattcttgct cccaaaaaga
     2821 gtgtccagct tacaccaccc cgtttctttt accttaaaat ctttgtgctt tttagccagg
     2881 tcttcaaaac gggaggtaaa gtaaggccct tgggttaaat ataggtcaaa gtagtttctg
     2941 attcggaaat gtcccttttt ctctcccgct agcccatgga aaacctgaac ctttacacca
     3001 ctcaggtagt gaggaacttc gtttcctggc acaaaaatgg catcactttt aaaatcgtag
     3061 agggttttaa tggaattggt tactattgat tcgtttttga aagggaattt atcagctatt
     3121 ctgtccggaa cataccagag aacctcattt ccgcgggcaa taagctcatc gttcaatggt
     3181 tttagtatgc caaatgagta ggggtttatg caaaagagta tagctttcac agcaaaaagt
     3241 attgtttact tgttaaaaag tctgttggca ttctccagat cttccttaaa atcaatttct
     3301 aagcacttgt ggctggaaat atcaaccggt aaaactttaa taccttcctt ttcaattgca
     3361 agctcaagcc cgcgttcaaa gtaatcgtta tcgttacact cctctaggta tttgataaag
     3421 gtttttatat cgttactcga aataaagtta ataccaacag cttccccttt ggcatcgacc
     3481 actttcttgg aaatctcctt aataaaatta ttgctgtcaa ggttgtattt tacctcttcc
     3541 tctcccaccg cactattatt aacgcaaaca aatgattggt tctgctgaat tagcgggagc
     3601 aacattttta gtatttcaac atcgaaaaca acatcgccgt taagccataa aaccgaggtg
     3661 tttttatact tgtttaaagc ctttaatagg ctttttgatg tattagtaac atcaaaattt
     3721 tggttgtaga tgtaggttag gttagggaaa ctctccatta ttacatcctt tttgaaacca
     3781 acaacaacat taatgtcatc gatgtcaaaa taggtggtca gattatcgat ttgctgttgc
     3841 atgatcgatt ttcccgattt aagcactgtt aagggtttgg gcaaggggtt tcctagcctt
     3901 gagcctattc ctgcagcaag aattactatt ttcataggag taattttgtg gttaatgaaa
     3961 gcaataatac attaattttt aaaaaaatcc caaaaaggtt tattaagcct aagggatgta
     4021 actaattagt tcaatacaat caggtatgct tattgtttct ctttttacga tcgttaatgt
     4081 agagctgatg cagcttgatg tatttaagaa agctggtgta ggccgataag gagcttataa
     4141 caaacccatt gtatccgtcc agaaatccta gttttagaaa gtaagctttg aagaaacgcc
     4201 acgatgagtt aaaaattatt ttaaagatac tgctcttaac acccattcgg tagtactcat
     4261 ttgccgaaat tgtactgaac cggttagcct tgtcgatatg ctcctcgtag gtgtggaggc
     4321 tccagtgaag caggtcgccc ggtaaagtac atattttaga acccggttgc atttttactg
     4381 tttcgtgaat gttcaatccc ccccagcgcc cttttctgcg gtcaaacagc ctgatttttc
     4441 tatcggggta ccaattggaa tgataaatcc actgcccgca gtagttgttg agcctgctga
     4501 atttataggc atcgcacgtc caattatttt ttacctccag tattgatttt tcaagttcag
     4561 gcgacaaggc ctcatcggca tcaagggaaa ggatacaatc aaacgacgcc tgctctattg
     4621 caaagttttt ctgatcccga taacccgtaa aagtattttg aataaatctt gctccaaact
     4681 tttcgcagat actcctggtt gaatcggtag aaaaggaatc gaccaccaca atttcatcag
     4741 caaccttaag aacagaattt aaacacctct caatattcct ttcctcgttt aaagttataa
     4801 taacagctga aatttttggc attttcaaga attttgaaca gcaaatatat tgaaatttct
     4861 aaaacacttt ttagccccct tgttttgagt attaagtttc tgaataaatt ctatttttgc
     4921 accttaaata ttaactaaat gggtaataaa aatattccta atttgaccaa aggaaattta
     4981 gctagagcaa ggaaaatgct ttttgatgtt gtcgaggtgc ttgataatgc taacattgtt
     5041 taccatcttg aagggggaac ccttttaggc attgttagag atggcgacct actcccctgg
     5101 gatcatgatg tggatctgtc aatcatgatt accgataccc caaaattctt aaagatcaaa
     5161 aaggaattat accgcaaggg ctacaaggta acttcaaaga aaagcaaaca cgatattcat
     5221 ccctttagga agggggatta taaaatattt aaaatcaaga aaatgattcc ttcagtggtt
     5281 aagtggctat tcccaaggta taagaagaac tatgtagttt tagatatttt cattaaggtt
     5341 aacgacggta attactgcta ctggcaggct atgaataagc tcatgagggt aagcaataaa
     5401 tactatgctt catatgaaac agtgaactac cgtggagcaa gcctcaaggt gccaaacgat
     5461 tataaggatt accttaccca aaagtatggc gactggtcag tatccgttaa ggaatgggtt
     5521 tgcggtgagg atgaaaagac gatagttagt tagagtttag taatcctcat aaagtaaaac
     5581 tatcgtacat ttcgcctagt aaaatcatta cacctgttcc aatttttttg aatgatttca
     5641 ttgatataaa cctttcccat ttagttatcg caaaaaatta aagacccgac ttgcgccggg
     5701 tcttatttta tttaggtatt taaattttac agtaagctaa aggctgctgt tagccctgcc
     5761 attacaattg ataccatgct catgagtttg ataaggatgt tgaggctggg gcccgaggta
     5821 tccttgaagg gatcgccaac tgtatcgcct acaactgcag ccttgtggtt gttggatccc
     5881 ttgcctccaa agttcccttc ttcaatatac ttcttggcat tatcccaagc tccgcctgag
     5941 tttgccataa atattgcaag aacaaagcct gcggcaagac caccggcaag taatcccaaa
     6001 acacctgcta cgccaagaac caagccggta acaatgggta aaattattgc caaaagcgat
     6061 ggcagaatca tctcgatttg agcaccgcga gttgaaatgg ccacgcagcg agcataatcg
     6121 ggttcggctt caccttccat gatgccctta atttcacgga actgacgacg aacttcctca
     6181 accatttttt gtgcagcacg gccaacagcg ttcatggtta agccactaaa caggaaggcc
     6241 atcatggctc caataaaaac gccaacaagc acaacggggt tcattaggtt aacctggtag
     6301 tagttcataa agtccacaat ggttgccttt gcggtatcaa tggtgtcgcc attggcaagt
     6361 gtaattacat tctcaccaat acggattaac ccaattttaa tctcctcaat gtaggaggct
     6421 aaaagcgcta gagcggttaa agctgcggag cctatggcaa atcctttacc agtagctgca
     6481 gttgtattac ccaaagcatc aagtgcatcg gtacgtttgc gaacctcggg gtctaagtgg
     6541 ctcatttcag cgttacctcc agcattgtcg gcaattggac cgtaagcatc ggtagccagg
     6601 gtaataccca gtgtagaaag cattccgaca gcggcaatac ctataccgta taggcctaac
     6661 tgaatgtttt gggcatcgag catgtgctta acatcgaaat tgatggccga aaggaatgca
     6721 attagtattg ctattgcaat ggtaataaca ggtatggcag ttgacaccat tcctaaaccc
     6781 acccctgaga tgataaccgt agctggtccg gtttgagcgc tttcagaaat cttgcgggta
     6841 ggcttatatg agtgcgaggt gaagtattcc gttgatagtc caattataat gccagccagc
     6901 aaccctgtaa caaccgagaa ggatattccc atccagttct gcaggcctaa tgcgtaaagg
     6961 attacaaagg taaggattgc aattagtgct gagctcacgt taacgcccac acccaacgag
     7021 cgaagcaggt cacgcatggt tgcgccctct ttggtacgaa ccatataaat gcctagaatg
     7081 gaaagcacta tgccaacact agcaattaac atgggtgcaa ggattgcttt gtactgcatg
     7141 tctactccgg ttgcagcaaa tgctgttgcc cctagggctg cagtggcaag aattgaccct
     7201 gcatatgatt catataagtc agcacccata ccggctacat cgcccacatt atcgcccacg
     7261 ttatcggcaa tggtggctgg gttgcggggg tcatcctcgg ggatacctgc ctcaacctta
     7321 cctacaaggt cggcacccac atcagcggcc ttagtataaa taccaccacc cacacgggca
     7381 aacagtgctt gggttgaggc acccattcca aaggtaagca ttgtggtggt aatgatgatt
     7441 aacttatgag agctatcggg ttcatcgata aagtagttga ggatcagata ccataaggaa
     7501 atatcgagta ctgccagacc aaccacaacc aatcccatta ctgcacccga cctgaatgct
     7561 accctaaggc catggtttag ggagttctta gcagcgttag ccgttcgagc cgatgcatag
     7621 gtggcggttt tcattccaaa aaatccggct aaaccggaaa agaaaccacc ggttaggaat
     7681 gcaaacggtg tccatttgtt ttgcactccc agtccaaatg ccaagaaaac aaaaagtaac
     7741 atcagcacga tgaatacaat aaccaccacc ttgtactgct gttttaggta cgacatggca
     7801 cctgtacgta cgtgctgtgc aatctttttc atcaggtcag taccctcatc ttccctcatc
     7861 atttgcctaa aaaaatggta ggcaaacgcc agggcaatca ccgatgcaat cggtacaatc
     7921 cagaacaaat gatcaataat cataggttta ataatttagg ttattatcga tatatttaga
     7981 tagataattt tcatgtaaac atttagctaa tatacaaagt gtaatttaag gtgagtatat
     8041 tcaaacaaaa aaaagaggtt gacttaaaag ttttttcatt aacaaatttt attaaaagat
     8101 gttcgttgaa agattcaata actttggttt ggtagaattt aaattctgtt ttcaatttcg
     8161 tttagttacc aaaagtaaac caccatttgt caaactgatc tagtcgaagg gttcagtgta
     8221 caggggaagt aaggataaag gataaaggtt aaaggataaa ggataaaaat ttaaaatttc
     8281 agatttcaga ttcatgattt aaggtggttt cttaaaccat caactatcaa ctatcaacca
     8341 tcaactaaat tgatatcaca gtgtaaatct gcctaatctg tgtcatctgc gttccatctc
     8401 atttcacgaa caacgattca cgaacaacga acaacgcatc ctgccctgta ggggctaaac
     8461 atttgtaatt ccataactac tcccatatat atagcgatgc acgtaataca ttttccaaag
     8521 agcaaggaca aaaagttgca tcgcaacaga aaggcaatgc aaagagcgat ttacacgttc
     8581 tttcttgtct tgatacaaga aagaaccaaa gaaa
//
LOCUS       DAOOWJ010000110         8418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_8391_length_8418_cov_7.314720, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000110 DAOOWJ010000000
VERSION     DAOOWJ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8391_length_8418_cov_7.314720"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1114
                     /locus_tag="PLW22_09800"
     CDS             11..1114
                     /locus_tag="PLW22_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOU75036.1"
                     /translation="MANEKLKTEEGTNPSIQKDAKPLIIAGPCSAESPQQLVEVAQEL
                     KKNGFVNYMRAGVWKPRTTPNSFEGYGTEALIWLNQVKQETGMPFATEVATERHVYEC
                     LKFGADLLWIGARTVSNPFAIQEIASALRGVQIPVLVKNPLSPDLKLWEGAINRLRLA
                     GVKEVGAIHRGFTVWHASPFRNFPMWEVALQLKANNPELRVISDPSHISGDAQYIELL
                     SNRAINLGFDGLMVEVHPNPKIALSDAKQQLTPNQFNLLISNIFEAKQANTELMNELR
                     AEIDALDELLVWALSNRMAIATEIARVKSETGQKIIQPDRWNEVLNRVIGKGHKMGLR
                     TDFVKRLFNSIHRESLQTQEAITQNRTEMVKFG"
     gene            1141..2160
                     /gene="aroB"
                     /locus_tag="PLW22_09805"
     CDS             1141..2160
                     /gene="aroB"
                     /locus_tag="PLW22_09805"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOU75037.1"
                     /translation="METILIEGQNGKSTIIVDERFEEVTKYLPKKKHFIITDKNIEKY
                     YATVFPEAPFFSINPGEPSKQLGYIESIYRWLLENNADRNSFILGIGGGVVCDIAGFV
                     ASTYMRGVEFGFVSTSLLSQVDASVGGKNGVDLDGYKNIIGTFTQPRFVLCDTRLLKT
                     LPHEELVNGFAEMVKHALISDIKLFEQLEHDCIALLNLNQPEISKHIAESIRVKASIV
                     TADERESGIRKLLNFGHTWGHAVETLTGISHGKAVSIGMEFACRLSMEKGLISNRDYL
                     RIINLLQSFGLPTCMNIEPSRVFKTLVKDKKRNANSIDFVLLMGIGVPKIETMALEEL
                     KKFAE"
     gene            2173..2826
                     /locus_tag="PLW22_09810"
     CDS             2173..2826
                     /locus_tag="PLW22_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOU75038.1"
                     /translation="MKAIRPDICVSIGNVDFHAISNLLPKLSLAEIRIDLLNLNRDEL
                     KLLFGLHDNLIATYRPKESEFDVMLNLLTDTIGYGAAWVDVDIDTPTEIIREITAIAQ
                     AANCKVIISYHNYTETPTYARLKKITENAQTYDVSLVKIACMANSTSDCARVLSLYEE
                     NTRIVAFCMGQVGTITRVAAPLLGAPFTYAALPGNQTAPGQLDYIYLESLLDSLKPL"
     gene            2828..3748
                     /locus_tag="PLW22_09815"
     CDS             2828..3748
                     /locus_tag="PLW22_09815"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOU75039.1"
                     /translation="METYAVFGNPILHSKSPQLFNSVFRGMGIDAKYTRIRPRSAHDL
                     TNIILNYSIKGANITTPFKEDVIKYLNHVSDEVAEIHGVNTITNQNGKLTGYNTDWNG
                     VVNALVNAGVTLIGRKVLVLGAGPAATAAVYGLKKAGAVVHIANRTTQKAIEIGKRLN
                     VNYIDFDEVSHKLSYFEVVVSALLPNANPFLGVNIPRNLVLLDANYRPSSLSEQFMSF
                     GCRVISGKQWLIHQAVEAFKIFTGKAPDVEIMSVAVENELTLNYLKAQWIQRVDNKKL
                     FTDYDLLISASNDNEFKQFLDEEISKTFGS"
     gene            3717..4964
                     /gene="aroA"
                     /locus_tag="PLW22_09820"
     CDS             3717..4964
                     /gene="aroA"
                     /locus_tag="PLW22_09820"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOU75040.1"
                     /translation="MKKLVKPSAVKGTVQAPASKSVAQRAIALATLAEGQSIIYNAGN
                     SNDCLAAIDTCKKLGAHIVGDGSKLTITGGPKSPNDTLHCGESGLSIRMFAAIASIFN
                     TPVTLTGSGSLTNRPMNMVTDGLAQLGVRCTTNNGKLPITVQGPIQGGYITVDGSQSS
                     QALTGLLMASPFAQNDVTIKVDNLNSKPYIDLTIETMKAFGVRVDELPGNTYRIAVNQ
                     KYKASNFNVEGDWSGAAFMLVAGAIAGEVEVININPKSLQADRAIVEALLSAGASLSI
                     RENSIVAKRANLKAFDFDARNCPDLFPPLVALAANCNGQSKILGVNRLHGKESDRALT
                     LQQEFGKMGVKIELVGNLMVVNGTSIKSAITYSHGDHRIAMACAVAALSANGDVEIEN
                     AEAVNKSYPNFFDDLYKMQVDNF"
     gene            4974..5960
                     /locus_tag="PLW22_09825"
     CDS             4974..5960
                     /locus_tag="PLW22_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOU75041.1"
                     /translation="MNTFGRIVRVSIFGESHGAMVGVTIDGLPHGIKLNADDFTDDLA
                     RRRAGAIGTTPRVEGDIPEIATGWFNGYTTGAPLTILFRNENTKSGDYDKLKDVPRPG
                     HSDFVAYHKFDGYNDYRGGGHFSGRLTLGLVAAGVVAKKVIPQVSIEARLIEAGGKTN
                     IDEAIAEAQKQNDSIGGIVECVATGIPIGLGEPFFDSAESVISHLAFSIPAVKGIEFG
                     SGFAASRMKGSEHNDPIIDSKGTTATNNAGGVNGGITNGNPLVFRIAVKPTSSIAKPQ
                     QTLNIKSEKIETLMVEGRHDTCIALRVPVVVEAITAIALADMLLIERTRKNR"
     gene            complement(5990..7210)
                     /locus_tag="PLW22_09830"
     CDS             complement(5990..7210)
                     /locus_tag="PLW22_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU75042.1"
                     /translation="MPKHYKIGILRETKTPPDRRVALTPEQVVEVIKKFDNVDVVVQP
                     SELRCYKDDEYLRLGVKLQDDLSDCDLLVGVKETKIPTIIPGKTYMEFAHVAKKQPHN
                     QKLIQAFAANGNTVIDYEYLTDKNGVRLVAFGHWAGVVGSYNALRAIYIKHKLGELPP
                     AHTLHDYNELKGILKKIKLPPLKFVITGGGRVAGGAIEVLGQAGIAEATHEEFLTKEF
                     DHPVYTRLDPWHYAKRNDGKPFEWDYWVNNPADHQSTFLPYAQTADVFIACHYWDFRS
                     PHFFTVDDMKQPDFRISIIADVSCDIPGPIPSTIKASTIADPFFDFNPATGKEEPAFS
                     CGMNVTVMSIDNLPGELPRDASEFFGRLLIDKVLPHLLCDDCEGVIERATILKDGKLT
                     PRYAYLQDYLEGKE"
     gene            7526..>8418
                     /locus_tag="PLW22_09835"
     CDS             7526..>8418
                     /locus_tag="PLW22_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU75043.1"
                     /translation="MGKEKKFITCDGNYAAAHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WSAFGRKNIFGDTVKVVEMQSEAGAAGAVHGSLQSGALTSTFTASQGLLLMIPNMYKI
                     SGELLPGVFHVSARSIAAQALSIFGDHSDVMSTRQCGFAMLATGGVQEVMDLAGVAHL
                     AAIKTRVPFLHFFDGFRTSHEIQKIEVLENNDLAPLIDQKALQAFRDRALNPEHPVTR
                     GTAQNPDIYFQTREAANKFYDAVPDVVEAYMQEITKLTGREYHPFNYYGDPNAENIII
                     AMGSVTDTLKETVDYLMSKGE"
BASE COUNT         2506 a         1819 c         1920 g         2173 t
ORIGIN      
        1 atactaaacc atggcaaacg aaaagttaaa aactgaagaa ggcactaacc catcaatcca
       61 aaaagatgcc aagccactga ttattgcagg gccttgcagt gctgagtcgc cacaacaact
      121 ggtagaagtg gctcaggaac ttaaaaagaa tgggtttgtt aactacatgc gggctggagt
      181 atggaagccg cgcacaaccc ctaactcatt tgagggctat ggaactgaag ccctaatctg
      241 gcttaaccag gtaaagcagg aaaccggtat gccctttgcc accgaggtgg caaccgagag
      301 gcatgtttac gagtgcctga agttcggtgc cgatctgcta tggattggtg cacgcaccgt
      361 ttccaatccc tttgctatac aggaaattgc atcggctttg aggggtgttc aaatcccggt
      421 tctggtaaaa aatccgttaa gccccgacct aaagctctgg gagggtgcaa ttaaccgact
      481 aaggctggca ggagtaaaag aggttggcgc catacaccgg gggtttaccg tttggcacgc
      541 atcgcctttc cgaaacttcc caatgtggga ggtagcccta cagctaaaag ctaacaatcc
      601 tgagctcagg gttatttcag accctagcca tatctctggc gatgcgcagt atattgagct
      661 actctcaaac cgcgcaatca accttggatt cgacgggcta atggttgaag tacatccaaa
      721 ccctaagata gcccttagcg atgccaagca acagctaaca cccaatcaat ttaacttgtt
      781 gattagcaat atttttgagg caaaacaggc taatacagag ctaatgaatg agctaagagc
      841 tgaaattgat gccttggatg aattacttgt ttgggcattg tcaaaccgga tggcgatagc
      901 aactgaaata gcccgcgtta aaagtgaaac gggtcaaaaa atcattcaac ccgaccgctg
      961 gaacgaggta ttaaacaggg tgattgggaa gggacataaa atggggctca gaactgattt
     1021 tgtgaaaaga ctttttaata gcatccatcg cgaatcgtta caaacacagg aagcaatcac
     1081 ccaaaacagg actgaaatgg taaaatttgg ttaactttgg cataatcctt aaatgcaaga
     1141 atggaaacca ttttaattga aggccaaaac ggtaaatcaa caataattgt tgacgaaaga
     1201 tttgaggaag ttaccaaata tcttcctaaa aaaaagcatt tcattattac cgacaaaaac
     1261 attgaaaagt attatgccac ggtgtttcca gaggcaccct ttttctcaat taatccgggt
     1321 gaaccatcaa aacagttagg gtatattgag agtatttacc gctggttgtt ggagaacaac
     1381 gccgatcgta atagctttat actgggaatt ggaggaggtg tagtttgcga tattgctggt
     1441 tttgtggctt ccacctacat gcgtggggtt gagtttgggt ttgtgtcaac cagcttactt
     1501 tcacaggttg atgcaagtgt aggcggcaaa aatggggttg accttgatgg atataagaat
     1561 attattggaa cgtttaccca gccccgattt gtgctttgcg atactcgctt gctaaaaaca
     1621 ttgccccacg aggaacttgt gaatggtttc gccgagatgg taaagcatgc cctaatctcg
     1681 gatatcaaac tttttgaaca gcttgagcac gactgtattg ctctgcttaa cctaaatcaa
     1741 cctgaaatat caaaacatat agcggaatcc attagagtta aagcaagcat tgttactgcc
     1801 gatgaacgtg aaagcggaat tagaaagctg ctcaattttg gccatacatg gggtcacgcc
     1861 gttgaaacac taaccggaat atcgcacggt aaggcggtta gcattggaat ggagtttgcc
     1921 tgcaggctct ccatggagaa aggacttatt agcaacaggg actacctgag aattattaac
     1981 ctgctacaat catttggact cccaacctgc atgaacattg aacccagtag ggtattcaaa
     2041 actctggtaa aggacaaaaa gcggaatgcc aacagtatcg actttgttct tttaatggga
     2101 attggagtcc ctaaaattga aacaatggca ttagaggaac taaaaaagtt tgcagaataa
     2161 aggttatgat gcatgaaagc tatccgcccc gatatatgtg ttagtatagg aaatgttgat
     2221 ttccatgcaa ttagcaattt gctacccaaa ctttcgcttg ccgaaatcag gattgatttg
     2281 ctcaatttga acagggatga gctaaagctg ctatttggct tacacgataa ccttattgca
     2341 acctaccggc ctaaagagtc agaattcgat gtaatgctta atctccttac tgatactatt
     2401 gggtatggcg cagcctgggt tgatgttgat attgacacgc ctactgaaat tatcagggaa
     2461 ataacagcta tagcccaagc cgctaactgc aaggtaataa tctcatacca taactatact
     2521 gaaaccccta catatgctag gctaaaaaaa attactgaga atgcccaaac ctatgatgta
     2581 tctttagtta aaatagcctg tatggctaac agtactagcg attgcgcccg cgtcctttcc
     2641 ctttatgaag aaaataccag aatagttgct ttttgtatgg ggcaggttgg aacaattact
     2701 cgcgttgcgg ctccattact aggcgccccg ttcacctatg cagcattacc cggaaatcaa
     2761 actgcaccgg gtcagcttga ctatatatac ttagaatccc tacttgactc cctaaaaccc
     2821 ctatagtatg gaaacctacg cagtttttgg taatcctata ctgcacagca aaagcccaca
     2881 gctattcaat agcgttttca ggggaatggg tatcgatgcc aaatacacca ggattcgccc
     2941 cagaagtgca catgacctaa ccaacatcat cctaaactat agtattaaag gtgctaatat
     3001 taccacaccc tttaaggaag atgttatcaa atacctgaac catgttagcg atgaagtagc
     3061 cgaaatacac ggggtaaata ccataaccaa ccagaacggc aaacttaccg gttacaatac
     3121 cgattggaac ggtgttgtaa atgctttggt taacgcaggt gttaccctaa taggtaggaa
     3181 agtgctggta ttaggtgctg gtccagctgc aacagctgct gtatatggac tgaaaaaagc
     3241 aggtgcagtg gtgcatattg ccaacagaac cacccaaaaa gccattgaaa taggtaaaag
     3301 actaaacgta aattacatag attttgacga agtttcccat aaactctcct actttgaggt
     3361 agtagtttcg gctttactcc ctaacgcaaa cccttttttg ggggttaaca taccccgaaa
     3421 tctagttcta ctcgatgcca attaccgccc ttcgagccta agcgagcaat ttatgagctt
     3481 tgggtgtagg gtaatttcag gtaagcaatg gcttatccat caagcagtgg aggcgtttaa
     3541 aatttttact ggtaaagcgc ctgatgttga aatcatgagc gtagcggtag aaaatgagtt
     3601 aacacttaac tacctgaaag cccaatggat acaaagggta gataataaaa aattgtttac
     3661 tgattacgat ttacttatat cggcaagcaa cgataatgaa tttaagcaat tcttggatga
     3721 agaaattagt aaaaccttcg gcagttaaag ggacggttca agcgccagca tcgaaaagcg
     3781 ttgcccaacg ggcaattgca ttggcaacct tggccgaagg acaatcgata atttacaatg
     3841 caggcaacag taacgattgc cttgccgcca tcgacacttg taagaaactt ggtgcccata
     3901 tagtgggcga tgggagcaaa ctaaccatta cgggaggccc caaaagccct aatgatacat
     3961 tgcattgtgg cgaatcgggg ttaagcatca ggatgtttgc tgccattgca tccatattca
     4021 atacgcccgt aacccttacc ggtagcggct cattaaccaa ccgcccaatg aacatggtta
     4081 ccgatggttt agcgcagctt ggcgttaggt gtaccactaa caacgggaag ttaccaatta
     4141 ctgttcaggg acccatacag ggtggataca ttactgtaga tggctcacaa agttcacagg
     4201 cacttaccgg cttactcatg gcttcgcctt ttgcccaaaa cgatgtaacc attaaggttg
     4261 ataacttgaa tagcaaacca tatatcgacc ttaccataga aaccatgaag gcatttggcg
     4321 ttagggttga tgaactgcca ggcaacactt accgcatagc cgtaaaccag aaatataaag
     4381 ccagtaattt taacgtggag ggggactgga gtggagccgc attcatgctg gtggctgggg
     4441 caattgcagg tgaggttgag gttataaaca ttaacccaaa atcgttacaa gccgataggg
     4501 ctatagttga agccttactg agtgccggag cttcgctatc gattcgtgaa aacagcatag
     4561 tagccaaaag agccaacctg aaggctttcg attttgatgc acgtaactgt cctgaccttt
     4621 ttccgccact tgtggcgctt gcagctaact gcaatggaca gtcaaaaata cttggggtaa
     4681 accgtttaca cggaaaggaa tcggacagag cgcttaccct acagcaagag tttgggaaaa
     4741 tgggcgttaa aattgaactt gttggcaacc tgatggtagt aaatggaacg agtatcaaat
     4801 cggcaattac atactcgcat ggcgaccatc gcattgccat ggcatgtgct gttgcagcac
     4861 tttcagcaaa cggagatgtt gaaattgaga atgctgaggc ggtaaacaaa tcgtatccaa
     4921 acttttttga cgatctttac aaaatgcaag tcgataattt ttagggcaac catatgaaca
     4981 catttggtag aatagtcagg gtcagtatct ttggcgaatc gcatggagcc atggtaggtg
     5041 taactattga tggcttgcca catggaataa agctaaatgc tgacgacttt acagacgatc
     5101 tagcccgccg tagggctggt gcaataggaa ccactccccg tgttgagggc gatatacccg
     5161 agattgctac tggttggttt aacggctaca ctacaggtgc ccctttaaca atactcttca
     5221 ggaatgagaa tacaaaatcg ggcgattacg ataagttgaa agatgttccc cgtcccgggc
     5281 attccgattt tgttgcatac cataagtttg acggttacaa cgattaccgc gggggcggac
     5341 atttctcggg ccgactaacc cttgggctgg ttgcagccgg tgtggtggcc aaaaaggtaa
     5401 taccccaggt tagcattgaa gcaaggctca ttgaggcagg aggcaaaact aatattgacg
     5461 aagccattgc cgaagcccaa aagcaaaacg attccatagg tggaattgtg gaatgcgttg
     5521 ccacgggaat ccccataggc ctcggtgaac catttttcga ttcagctgaa tcggtaataa
     5581 gccatctggc gttttccatt cctgccgtaa aaggtattga gtttggatca ggctttgccg
     5641 cgtcgcgcat gaaaggctcg gaacataatg acccgataat tgatagtaaa ggaactacag
     5701 ctactaacaa tgccggaggg gtgaacggtg gtatcacaaa cggaaaccca cttgtgttcc
     5761 gaattgcggt taaaccaaca tcgagcattg ctaaaccgca gcaaaccctg aatataaaat
     5821 ccgaaaaaat tgaaactcta atggttgaag gcagacacga tacctgcatt gcgctaaggg
     5881 ttccggttgt agtagaggcc attacagcca ttgctttagc cgatatgcta ttaattgaaa
     5941 ggacaagaaa aaaccggtga ttatgccacc ggtttcaaat tcctaccatt cactctttcc
     6001 cttcgaggta atcctgaagg taagcatacc ggggtgttaa cttcccatct tttaggatag
     6061 tggctcgctc tatcacacct tcgcaatcgt cgcatagcag gtgcggtagc accttgtcga
     6121 tgagcaaacg gccaaaaaac tcactggcat cgcggggtaa ttcgcccggt aagttatcga
     6181 ttgacatcac ggtaacgttc atcccgcatg agaatgcggg ctcttccttt ccggtagctg
     6241 gattaaagtc gaagaaagga tcggcaatgg tggaggcctt tatggttgag ggtattggac
     6301 cgggtatatc gcagctcaca tcggcaatga ttgagattct gaaatcgggt tgtttcatat
     6361 catcaacggt gaagaagtgg ggtgaacgga aatcccagta gtggcaagca ataaacacat
     6421 cggcagtttg tgcatagggt aagaaagtac tctggtgatc ggctggatta ttcacccagt
     6481 aatcccattc aaagggttta ccgtcattgc gtttggcgta atgccatggg tcgaggcggg
     6541 tgtaaaccgg atgatcgaat tccttggtca ggaactcttc gtgtgtagct tcagctatac
     6601 cagcttgtcc aagcacttct atggcaccac ctgccaccct accaccaccg gtaattacaa
     6661 acttcaacgg gggtaacttg atttttttga gaatgccctt tagctcatta tagtcgtgca
     6721 acgtatgagc aggaggcaat tctcctaact tgtgtttaat gtaaattgcc cttagggcat
     6781 tatacgatcc aacaactcct gcccagtgac caaatgcaac taaacgaacg ccattcttat
     6841 cggtaagata ctcatagtca ataaccgtat tgccattagc cgcaaaagcc tgtatcagtt
     6901 tctgattgtg aggctgcttt ttagccacat gtgcaaattc catgtaagtt ttacccggaa
     6961 tgattgttgg tattttggtt tccttaacgc caacgagcaa gtcacaatcc gataaatcgt
     7021 cctggagctt taccccaagg cgcaagtatt catcatcctt atagcacctt aactcgctgg
     7081 gttgaaccac aacatcaaca ttgtcgaatt tcttaatcac ctcaacaacc tgctcggggg
     7141 ttaaggctac gcgcctatcg ggtggcgttt tggtttccct taaaattcca atcttataat
     7201 gtttaggcat atggcttggt tttaactttt tttagcaaaa attgaatttt gcgaaactaa
     7261 taaaacgata tcacatttga aaaaattttt aaatggatgt taaataaaag aattattgct
     7321 tacgatttat aacatatcga gcaaaaaata tatcgaattg taatattata ctccaataac
     7381 agatgtttat ttcaaacgtt ttcaggttgt tagcgtgtaa acacatcgct gtaaattgtt
     7441 gtaaagcatg aaaaaaatca ggtaatagta ttaactttgc atgggcttaa aaattattac
     7501 taaccatcat aacaatcaaa ctgaaatggg aaaagaaaaa aaatttatta cttgtgatgg
     7561 gaactatgct gctgcgcaca tagcatatat gtttagtgag gttgcagcta tttaccccat
     7621 caccccatcg tccaccatgg ccgagtatgt tgacgagtgg tcggcatttg gtcgaaagaa
     7681 catatttggc gacactgtaa aggtggttga aatgcaaagc gaggccggag ctgcaggagc
     7741 agtgcacggc tcattgcaat caggtgcatt aacatcaacc ttcactgcat cgcaagggtt
     7801 gttgctgatg atccccaaca tgtataagat ttcgggagag ttactcccgg gtgtattcca
     7861 cgtttcggct cgtagtattg cagcgcaagc gctatcgatc tttggcgatc acagcgacgt
     7921 gatgagtacc cgccagtgcg gatttgccat gctggcaact ggtggagtgc aggaagtaat
     7981 ggaccttgct ggtgtggctc accttgcagc tatcaaaacc cgagttcctt tccttcactt
     8041 cttcgacggt ttcagaacat cacacgaaat ccagaagatt gaagttttgg agaacaacga
     8101 ccttgccccg ctcattgacc agaaggcatt acaggcattc cgcgacaggg ctctcaaccc
     8161 tgaacatcct gttacacgcg gtacagccca aaaccccgat atctacttcc agactcgtga
     8221 ggctgctaac aagttctacg atgccgttcc cgatgttgtg gaagcataca tgcaggaaat
     8281 taccaagcta accggtcgcg agtaccaccc attcaactac tacggtgacc ccaacgctga
     8341 gaatatcatc attgcaatgg gttcagttac cgataccctt aaggaaaccg ttgactacct
     8401 gatgtcgaaa ggcgaaaa
//
LOCUS       DAOOWJ010000111         8391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_8427_length_8391_cov_6.904031, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000111 DAOOWJ010000000
VERSION     DAOOWJ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8427_length_8391_cov_6.904031"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /gene="wecB"
                     /locus_tag="PLW22_09840"
     CDS             complement(<1..1050)
                     /gene="wecB"
                     /locus_tag="PLW22_09840"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOU75044.1"
                     /translation="MGSKKVILTVLGARPQFVKAAAVSRQLQAFDIPEIIVHTGQHFD
                     QNMSDVFFSEMSIPAPAYNLNINSLNHGAMTGRMLELVEEVILKEKPSAVMVYGDTNS
                     TLAGALAAVKLHVRVIHVEAGLRSFNMAMPEEVNRILTDRISSLLFCPTDAAVANLKA
                     EGFDGFKAQIVKCGDVMQDAAYFYLDKAKRESKILNELSLSNDFVLATIHRQENTDDP
                     SNLKNIIDGLNSINRETRVVVPLHPRTRKILEQMGISPEFTAINPVGYFDMLMLISNC
                     KLVMTDSGGLQKEAFFFGKHCITLREQTEWVELVEHGFNQLAGPNADTIYHAYSEMIN
                     KRSNFDIDLYGNGKAS"
     gene            complement(1043..2356)
                     /locus_tag="PLW22_09845"
     CDS             complement(1043..2356)
                     /locus_tag="PLW22_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU75045.1"
                     /translation="MSQTSRKRVLIVTYYWPPSGGVAVQRWLKFVKYLRDFGWEPIVY
                     TPENPESFYDDHSLFKDIPENIEVIKRPIWEPYDIYKWITGRKGKKLGLGFADDSGKK
                     VGLAKLMVWIRGNILIPDPRVFWVRPSVKFLKRYLKQHPADAIVTTGPPHSMHLIGLN
                     LKRKLNIPWLADFRDPWTNIDFYHELRLTKFANWYHHRLERKVVKTADHVVVVSQQMK
                     REFESLGSNKVFVLTNGFDTDDIPAGETQSNDSFSICYVGTMNSARNPHVLWQALSRI
                     CAENDEFAQKLRIQMAGQIDISVTTAIKSYGLEKNFEHHGLLPHDRALALQKNAQILL
                     LVINNTPNAKGIVTGKFFEYLAVGKQILTIGPTDSDIAEIVHETASGDVIDYTDVEGA
                     TQTLVKYFNLFKQGNLTPEPKGIEKYSRKALTGRLSEILNQITNG"
     gene            2476..3432
                     /locus_tag="PLW22_09850"
     CDS             2476..3432
                     /locus_tag="PLW22_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HOU75046.1"
                     /translation="MVLFKVVAWLILVYGIIALVAKKARPSGFDLPFALPAILVGAIL
                     VVILSLIVVVGPQEVAVVTTPSGVRSEPLHTGWHVIAPWNDIHYMDKTVWVYTCANAI
                     GEGEKPNSDAIWAPTKDGIKLGFDVSVSWRIDANEAPWIYQNVTENDGGRSGRYLWLE
                     ENVIRAKLKSALALTVSGYTPIEAYSTKRNDIQQEILNRIKDECKQYRIIIDNVDVRE
                     VYYNEEYEKAINAKKLAEQEALRLIEVTKQKEEQLKQATIDKNIAIEKAKGESEALKI
                     KGQSISSNPKIIELEWINKWNGQLPTYMMGNGQGIMVNLNNK"
     gene            complement(3502..4023)
                     /locus_tag="PLW22_09855"
     CDS             complement(3502..4023)
                     /locus_tag="PLW22_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOU75047.1"
                     /translation="MLYIGCSDSRVTAEELMGANPGDVFVYRNIANLVPAGDLASMAI
                     INYAVTVLRVEHIVVCGHYHCGGIEAALQPKDMGILNPWLRNIRDIYRHHRHEIETSN
                     SITDRQRRLVELNVREQCVNVIKAPEVQKAIRSKTLNVHGWVFDMETGLLKDLKVDTD
                     AILKEIMSIYRLE"
     gene            complement(4132..5673)
                     /locus_tag="PLW22_09860"
     CDS             complement(4132..5673)
                     /locus_tag="PLW22_09860"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOU75048.1"
                     /translation="MKTLDELYQELERRNAIAEQAGGSDRIAKQHESGKKTARERIVE
                     LLDPGTFNEFDKFVTHRTNDFGMDKNRIPGDGVVTGYGKIDGRLVYVYAYDFTVFGGT
                     LSRANADKIIKLQQLATRMGAPIIGLNDSGGARIQEGVQSLAGYAEIFYNNVRSSGVI
                     PQVTCIMGPCAGGAVYSPALTDFIIMVKDSSYMFVTGPDVIKAVTHEDVTKDELGGAV
                     THNAKSGVAHFIAENDEQALMLVRELMSFLPVNNMEEPPVFPTTDDPLREDESLQTLI
                     PADPNKPYDIKELILTVVDDKNFLEVQPLFAKNIVVGFARMAGHTVGIVANQPAHLAG
                     VLDIDASVKGARFIRFCDSFNIPIVTFVDVPGFLPGVNQESNGIIRHGAKLLYAYSEA
                     TVPKIAVITRKAYGGAYCVMSSKQVGADVNYAYPMAEIAVMGPEGAVNILYRNKIDSE
                     ETRQELIDEYRETFANPYRAAELGYIDEVIYPKQTRAKLIQALEMTRTKSQQNPPRKH
                     GNIVL"
     gene            complement(5706..6215)
                     /locus_tag="PLW22_09865"
     CDS             complement(5706..6215)
                     /locus_tag="PLW22_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOU75049.1"
                     /translation="MGLEVRIGDRIAEVKLVRRDENIAVFEVDGVEYEVDIAQVENGV
                     FSIIHNGKSFNIEIIPGDGPKNYYVNTFYNSHEVEIIDAQSRYQRSRKALDDHAADKI
                     ISTPMPGRVVKIPVSEGETVSKGTTVIVISAMKMESEYKSPVDGIVKKIYVAEGDNIN
                     ANQPLVEIE"
     gene            complement(6233..7744)
                     /gene="accC"
                     /locus_tag="PLW22_09870"
     CDS             complement(6233..7744)
                     /gene="accC"
                     /locus_tag="PLW22_09870"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOU75050.1"
                     /translation="MIKKVLIANRGEIAVRVIRSCREMGILSVAVYSDADRRALHVRY
                     ADEAYHIGPSPSRESYLNGDKIIEVALKAGADAIHPGYGFLSENAEFARKCAAAGITF
                     IGPDPDAINQMGDKITARQTMIAAGVPVVPGTQQKVSDMETVRKIVDEIGLPVIIKAS
                     AGGGGKGMRLVRRPEDLISAVKMAQSEALSAFGDDTVYIEKYVESPHHIEFQILADRH
                     GNTVHLFERECSIQRRHQKVIEETPSPFLTPELREEMGKAAVAAAKAVNYVGAGTIEF
                     LVDGNRNFYFLEMNTRLQVEHPITERVTGIDLVKEQIRIASGLPISFRQDEIAQHGHA
                     IECRILAEDPFNNFMPSPGLIRHISEPNGLGVRTDGYAYEGYEIPIYYDPMISKLITW
                     GRDRTEAINRMKRALFEYKITGVKTSIRFLERIMDNHDFRMGNYNTHFIPEHLDELMD
                     IQPRGSKIFEDMAIIAAYFDFITKMEKVAMSDFTVTHSDNWKTYGLKRSLLPY"
BASE COUNT         2153 a         1951 c         1928 g         2359 t
ORIGIN      
        1 cgatgctttc ccatttccat acaaatcgat gtcgaagttc gatcttttat ttatcatttc
       61 actgtaagca tggtaaatgg tatcggcatt ggggcctgct aattgattaa agccatgctc
      121 aaccaattca acccactcgg tttgttcgcg cagagtaatg cagtgttttc caaaaaagaa
      181 agcctctttc tgtaaccctc cgctatcggt cataaccagc ttgcaattgc taataagcat
      241 cagcatatca aaatacccaa ccggattaat agcagtaaac tcaggggaaa ttcccatttg
      301 ctcaagtatt ttcctggttc ttggatgcaa tggaaccact acacgtgttt cacggttaat
      361 actattcagc ccatcaataa tgtttttgag gtttgatgga tcatcggtgt tctcctgcct
      421 gtgaatggtt gctaaaacaa agtcattgct gagcgatagc tcgtttaata ttttcgattc
      481 acgttttgct ttgtcgaggt agaaataggc cgcgtcctgc attacatcgc cacacttaac
      541 aatttgagct ttaaagccat caaagccttc ggctttaagg ttggcaaccg cggcatcggt
      601 tgggcagaat agaaggcttg aaatgcggtc ggtaagaatt cggttcacct cctcgggcat
      661 ggccatgttg aacgagcgta aaccggcttc aacgtggatt acccttacat gcaacttaac
      721 agcagcaagc gctccggcaa gggttgagtt tgtatcgcca taaaccataa cagccgatgg
      781 cttctccttc aggattacct cttcaaccag ctcgagcatc cgcccggtca ttgccccatg
      841 gttaaggctg ttgatgttca ggttgtaggc aggtgccggt atgctcatct cgctgaaaaa
      901 cacatcggac atgttttggt caaaatgctg accggtgtga acaattatct cgggaatgtc
      961 aaatgcctgc agttggcggc taacagccgc cgcctttaca aactgagggc gtgccccaag
     1021 aacagtaaga attacctttt tactacccat ttgtgatttg gtttaaaatc tcggacaacc
     1081 tgccggtaag ggctttacga gagtatttct caataccctt gggttcggga gtgaggttgc
     1141 cctgcttgaa taagttaaag tatttgacga gtgtttgagt ggccccctca acatcggtat
     1201 aatcaataac atcgccagag gccgtttcgt gcactatttc tgctatatcg ctatcggtgg
     1261 ggccaatggt aagaatttgc ttgccaacag caaggtactc aaagaacttc ccagtcacaa
     1321 ttccctttgc gtttggggta ttattgatga caagcagcag aatttgagcg tttttttgaa
     1381 gcgccaatgc ccggtcgtgt ggaagtaagc catggtgctc aaagttcttt tcaagcccat
     1441 aactctttat ggccgtagta accgagatat caatctggcc agccatttgt atcctcaact
     1501 tttgggcaaa ctcatcgttt tccgcgcata tcctacttaa cgcttgccat aaaacatgtg
     1561 ggttacgggc tgagttcatg gttcccacat agcaaatgga aaaactatcg ttactttggg
     1621 tttcaccagc agggatgtca tccgtatcaa agccgttggt taaaacgaaa actttattac
     1681 tgcctaagct ttcaaattcc cgtttcattt gctgcgaaac tacaacaacg tgatcggcag
     1741 tttttacaac tttccgctca agcctatggt gataccagtt ggcaaacttg gtaagcctca
     1801 gctcatggta aaaatcgata tttgtccatg ggtctcggaa atcggcaagc catggtatat
     1861 tgagtttacg cttcagatta agtccaatta gatgcatgga gtgaggtggc cctgtggtaa
     1921 caatggcatc agctgggtgt tgcttaaggt aacgctttaa aaactttacc gatggcctaa
     1981 cccagaatac gcgcggatcg ggtatcaaga tgttaccccg tatccaaacc atcagctttg
     2041 ccaaacccac ttttttaccg ctatcgtcgg caaagccaag tcctaacttt ttcccttttc
     2101 gccctgttat ccatttgtaa atgtcgtagg gttcccaaat tggccgttta attacctcaa
     2161 tgttctcagg gatatcttta aacagggaat ggtcgtcgta aaaggattca gggttctcag
     2221 gggtgtaaac aattggctcc cagccaaaat cgcgaaggta ctttacaaac tttagccaac
     2281 gttgcacggc aacaccaccc gatggaggcc agtagtatgt tactatcagt acccttttac
     2341 gcgatgtttg actcatacaa aaatttttgt taaagataga tagattagcc aattccaaca
     2401 aatgggggca tcacttgatt gtcaattcaa atgtttttca tacctttcct aatctaactt
     2461 aaaatgcata agtgtatggt actctttaaa gttgttgcgt ggttaatcct tgtttatggc
     2521 ataattgcat tggttgccaa aaaggcaagg ccatcggggt ttgaccttcc ctttgctttg
     2581 ccagccattt tggttggtgc tatacttgta gtgatacttt cgctgattgt ggttgttggg
     2641 ccgcaggagg ttgctgtggt taccacacca agtggtgtcc gctctgaacc tctgcacacc
     2701 ggatggcatg taatagcccc gtggaacgat attcactata tggataaaac cgtttgggtt
     2761 tacacctgtg ccaatgctat tggcgagggc gaaaaaccta actccgatgc tatttgggca
     2821 cctaccaagg acggtattaa gcttggtttt gatgtatccg tttcgtggcg cattgatgcc
     2881 aatgaagctc cttggatata ccaaaacgtt acggaaaatg acggtggccg atcgggacgt
     2941 tacctctggc ttgaggagaa tgtaatccgc gcaaagctga aatcggcatt agctctaacc
     3001 gtttcgggtt atactcccat tgaggcatac agcactaagc ggaacgacat ccaacaggaa
     3061 atccttaacc gcattaagga tgaatgcaag cagtatcgaa taataattga taacgtggat
     3121 gtgcgcgagg tttactataa cgaggagtac gaaaaggcta ttaatgctaa aaaattagct
     3181 gagcaggaag ccttaaggtt aattgaggta accaagcaaa aggaagagca gctcaagcag
     3241 gctactatcg ataagaatat tgccattgag aaagctaagg gagagtcgga ggctcttaaa
     3301 atcaagggtc agtccattag tagcaatcct aagatcattg aacttgaatg gatcaataag
     3361 tggaatggtc agcttcccac atacatgatg gggaatggcc agggaataat ggtcaacctt
     3421 aacaacaagt agtaacccct aatgaactag cgaagccggt aatcccggct tcgtttgcta
     3481 tgatttctgc aaagaaaata tctattcaag gcgatatatc gacataattt ccttaagtat
     3541 agcatcggta tcgaccttta aatctttcag caggcccgtt tccatatcga aaacccaacc
     3601 atgcacgttg agtgttttgg aacggatagc tttttgaact tctggggcct tgataacgtt
     3661 tacgcattgc tccctcacgt tcagttccac caaacgtctt tgtctatcgg taatagagtt
     3721 gcttgtttca atttcatgtc gatggtggcg ataaatatca cgtatattcc gtagccaggg
     3781 attgagtata cccatatctt taggctgcag tgcagcctca atgcctccac agtggtagtg
     3841 accgcaaact acaatatgtt caaccctaag taccgttaca gcatagttta ttattgccat
     3901 tgaggcaagg tcgccagcgg gaacaaggtt ggcaatatta cggtaaacaa aaacatcgcc
     3961 cggatttgcc cccataagct cttcagctgt aacccggcta tcggaacagc caatgtaaag
     4021 cattttgggt tgctggttta gcgaaaggtt tcgaaagaaa ctggggttta cgcgcttgcg
     4081 cgcttcggcc cagttcctgt tattctcaaa aacctttttg atgtccctga actacaaaac
     4141 aatattgcca tgcttgcggg gtgggttctg ctggctcttg gtacgggtca tttccaatgc
     4201 ctgaatcagc ttggcacgag tttgctttgg gtagataacc tcatcgatat agcccagttc
     4261 cgcagcacgg taggggtttg caaatgtctc gcggtactca tcaattagtt cctgacgggt
     4321 ttcctcgctg tcaattttat tcctgtacag tatgtttact gctccctcgg ggcccataac
     4381 agcaatctca gccatgggat aggcgtagtt aacatcggcg ccaacctgct tgctggacat
     4441 tacacagtat gcaccaccgt atgccttgcg ggtgattacc gcaattttag gaactgttgc
     4501 ttccgagtaa gcgtatagca gctttgctcc atggcgaata ataccattcg attcctgatt
     4561 aacacccggt agaaatcccg gaacatcaac aaaggttaca atgggaatgt tgaacgagtc
     4621 gcagaagcga ataaagcggg cgcccttaac cgatgcgtcg atatcgagca cgccggcaag
     4681 atgggcaggc tggttggcaa ctatgccaac ggtgtgtccc gccatacgtg caaagccaac
     4741 aacaatgttt ttggcaaaga gcggctgaac ctccagaaag tttttatcgt caaccacagt
     4801 taggattaat tccttgatat cgtagggttt atttgggtcc gctgggatta gggtttgtag
     4861 actctcgtcc tcacggagcg ggtcatcggt tgttgggaaa acgggtggct cctccatgtt
     4921 gttaacgggg aggaaactca ttagctccct aacaagcata agcgcctgtt cgtcgttttc
     4981 ggcaatgaag tgcgctacgc cgcttttggc attatgggta acggctccgc ccagctcatc
     5041 cttggtaaca tcctcgtgtg taacggcttt tatcacatcg ggtccggtaa caaacatgta
     5101 gctggaatcc ttaaccataa taataaaatc ggttagggct ggtgagtaca ctgcaccacc
     5161 agcgcaaggt cccattatgc aggttacttg tgggataacc ccggaactac gaacgttgtt
     5221 gtagaatatc tcggcatagc cagccaaact ctgaacacct tcctgaatac gtgcaccacc
     5281 cgagtcgttc aacccaataa ttggtgcccc catacgggtg gccagctgct gaagcttgat
     5341 aatcttatcg gcgttagccc tactaagcgt accgccaaaa acagtaaaat cgtatgcata
     5401 aacgtaaacc aaccgtccgt caattttgcc ataaccggtt accacgccgt cgccgggtat
     5461 tcggttctta tccatgccaa aatcattggt acggtgggtc acaaacttgt caaattcatt
     5521 gaaagtaccg gggtcgagca gttcaacaat acgttccctt gcagtttttt tcccactttc
     5581 gtgttgtttg gcaatacggt cggatccgcc agcctgctcg gctatggcat tgcgtcgctc
     5641 taactcctga tatagctcat caagtgtttt catggaaaaa aataataaag gtttaaggat
     5701 agaatttact caatctcaac aagcggttgg ttggcattga tattatcgcc ctcagccacg
     5761 tatatcttct ttacaatccc atcaacgggg cttttgtact cgctttccat tttcatggcc
     5821 gatataacaa tcaccgttgt tccttttgaa acggtttcgc cttcggatac cggaatctta
     5881 accaccctac cgggcatagg tgttgagatt atcttatcgg cagcatggtc gtcgagagcc
     5941 tttcggcttc gttggtatcg gctttgggcg tcaataattt ccacctcgtg ggagttgtaa
     6001 aaggtattca cgtagtaatt ttttggccca tcgccaggaa taatctcaat gttaaacgat
     6061 ttgccattat ggattatgga aaaaacaccg ttctcaacct gggcaatgtc cacctcgtac
     6121 tcaacgccat caacctcaaa aacggcaata ttttcatcac ggcgcaccag cttaacctcg
     6181 gctatgcgat cgccaattct aacttcaagt cccattgttc aagcatgttt gtttagtaag
     6241 gaagcaagct gcgctttaac ccgtatgttt tccagttatc ggagtgggtt acggtaaaat
     6301 cgctcatagc caccttttcc atcttggtga taaaatcgaa ataggctgca attattgcca
     6361 tgtcctcaaa gattttacta ccgcgaggct gaatatccat cagctcatca aggtgttcag
     6421 gtatgaagtg ggtgttgtag ttacccatcc taaagtcgtg gttatccatg atacgctcaa
     6481 ggaagcggat ggatgttttc accccggtga ttttgtactc aaacagagcc cgtttcattc
     6541 ggttgatggc ctcggtacgg tcgcgccccc aagtgataag cttgctaatc atggggtcgt
     6601 agtaaatagg aatttcgtag ccctcgtaag catagccatc ggttctaaca ccaagcccat
     6661 tgggctcgct gatatgcctg ataaggccgg gcgatggcat aaagttgttg aacggatcct
     6721 cggcaagtat gcggcactca atggcatgcc cgtgctgggc tatctcatcc tgcctgaacg
     6781 atattggtaa tccggaagca attcggattt gctccttaac aaggtcgatt ccggttacac
     6841 gctcggtaat agggtgttca acctgtaggc gggtgttcat ttcaaggaag tagaagttgc
     6901 ggttgccatc aactaggaac tcaatggtac ctgcaccaac atagtttacc gctttagcgg
     6961 cagccacggc tgctttcccc atttcctccc taagctcagg ggtaaggaag ggcgaggggg
     7021 tctcctcgat caccttttgg tggcggcgct gtatggagca ctcgcgttca aagaggtgaa
     7081 cagtattgcc atgcctgtcg gcaagtatct gaaactcgat gtggtggggc gattcaacat
     7141 acttctcaat atacactgta tcgtcgccaa aggccgacaa tgcctccgat tgagccattt
     7201 taacggccga aataagatct tcgggacggc gaaccagtcg cattcccttg cctccaccac
     7261 cggccgaggc tttaattata acgggcaaac caatctcatc aacaattttg cgaacggttt
     7321 ccatgtccga aaccttttgc tgggttccgg gtaccacggg tacgccggca gcaatcatgg
     7381 tttggcgggc ggttatctta tcgcccattt ggttaatggc atcggggtcg ggcccaataa
     7441 aggttatgcc tgcggcggcg catttacggg caaactcagc gttttccgaa aggaaaccgt
     7501 agccagggtg aatggcatcg gcacctgcct taagggccac ctcaataatc ttatcgccat
     7561 tcaggtagct ctcgcgcgag ggcgatggcc caatgtggta tgcctcatcg gcatagcgca
     7621 catggagtgc acgtcggtcg gcatcggagt aaaccgcaac ggagagtatc cccatctcgc
     7681 ggcatgatcg gataacccgg actgcaatct cgccccggtt agcaattaat acttttttaa
     7741 tcatagttgt cccagatatg aatccgaggc aaaggtatgg aaaaaaatat tttttgtgca
     7801 agcattttta tacaaatatg ttgttcggca taattttata gcatgcttgt ttttgctgca
     7861 aacgttttaa atcaacactt tcggtaaaac gttcgtttga ttatttttaa tcgttctaaa
     7921 ggaagttgat ggttttgggt ttgctataac acagaatacg cagagttaca cagtgtaaca
     7981 tcgtgaaaca ctgtgcaact ctgtgatact tgctgctgtt tagttcacga gacgattatc
     8041 atgctgagct agccgaagca tccctttgta ccttcattcc ctttctcgct tttgattggt
     8101 tttccggcaa tcgctttttg ggattttttt ccattgcgat gcaacgcttc accctggctc
     8161 ttcggaatgc catgccgtgc atcgctatac atttaggatt aactataggt ttacaaatgt
     8221 ttcgccccta cggggctgga aaggttgatc gttgttcgtt gatcgttgat cgttgttcgt
     8281 aaaagagcct gaaccctaaa catccatcca ttacccttta tcatttatcc ttaaggtttg
     8341 tctagtcctg aaataagtct acaggttaga aatagattta agcgacacac a
//
LOCUS       DAOOWJ010000112         8294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_8563_length_8294_cov_8.054618, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000112 DAOOWJ010000000
VERSION     DAOOWJ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8563_length_8294_cov_8.054618"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..186)
                     /locus_tag="PLW22_09875"
     tRNA            complement(113..186)
                     /locus_tag="PLW22_09875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Arg,seq:acg)
     gene            complement(271..639)
                     /gene="rplS"
                     /locus_tag="PLW22_09880"
     CDS             complement(271..639)
                     /gene="rplS"
                     /locus_tag="PLW22_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOU75051.1"
                     /translation="MDLIKIAEQAFVEKKNHPDFKSGDTVVVHYKIKEGNKERIQNFR
                     GVVIQRKGTGATETFTVRKISGGVGVERIFPLHSPFIEQIEVAKEGRVRRARIYYLRN
                     LTGKKARIKEKKRGVEGENM"
     gene            complement(813..1904)
                     /gene="hydE"
                     /locus_tag="PLW22_09885"
     CDS             complement(813..1904)
                     /gene="hydE"
                     /locus_tag="PLW22_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOU75052.1"
                     /translation="MIIDNLLNKDNLNKEELVYLLGCEGEEKTKLFTKAAEVKAKYIG
                     RKVYFRGLIEMSNICRKNCLYCGIRSGNTNVHRYNLTDDEVIEAAKFAWENRYGSIAI
                     QTGEVSTKEFVDRIENLLDKINKATNHELGITLSLGEQTEETYRRWFNKGAIRYLLRI
                     EASNRELYAKLHPNDKHHDFDERLECLRALRRVGYQVGTGVMIGLPFQTLEHLADDLL
                     WMQEIDVDMVGMGPYVEHPDTPLYQYRDKLLPLTERFNLALKMTALLRILMKDINIAA
                     PTALQAIDPLGREKAIKVGANIVMPNITPGAYRNDYKLYENKPCTDDAADDCKNCLEA
                     RIQLTGDEIGYGEHGNSRHFYRRRNDTTI"
     gene            1986..2678
                     /locus_tag="PLW22_09890"
     CDS             1986..2678
                     /locus_tag="PLW22_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75053.1"
                     /translation="MERAKAIGQIIAVIKQLKKETDELFAPENIINHSELLIEEYFQQ
                     LFNPFLKKVKADIRSLPLDEYLPIELDSWIRILKMKLVAIERGEHTEKYAEVLYIKQR
                     FYPELIDVLKELQLEALEAEFDARVDREQLSKPILGPNTIVLSIESVAMLITMLYNFN
                     IISGAVPIDDICRSFAAMTGYNAGELKQLLRFDEENGRLTSIVKAEDMDVLLKLLRSI
                     VARIENLRQYTD"
     gene            complement(2675..3070)
                     /locus_tag="PLW22_09895"
     CDS             complement(2675..3070)
                     /locus_tag="PLW22_09895"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU75054.1"
                     /translation="MKALRIEAEIDSPEIYFNPDTKVFSISGISHPENAKEFYQQILD
                     WLDEYYEFIKNQEPSKIIVDLNFRYINSTSYKYLRDVLRKISSFRSANFEVEVIWNYH
                     EEDEELLHEGEVLFELPDINLPYKCIPYN"
     gene            complement(3391..>3629)
                     /locus_tag="PLW22_09900"
     CDS             complement(3391..>3629)
                     /locus_tag="PLW22_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOU75055.1"
                     /translation="SWGIALFEYFFQVPANRLGFRENGGPFSLVELKVIQEAITLVVF
                     MGFTLFAFKTETIRTNHIIGFGFLILAVYFIFKK"
     assembly_gap    3630..3729
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3763..4629
                     /locus_tag="PLW22_09905"
     CDS             3763..4629
                     /locus_tag="PLW22_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOU75056.1"
                     /translation="MTGYGKAERVFGNKKIVVEVRSLNSKQFDFNLRLPAIYRELEME
                     IRTKAAKELERGKVDMSISIEETAESQAIPINDSVFMAYYRQLSDIASKNSISFNPAE
                     VVPAILRLPEVIRAQKEEIAPEELDAVLQACAEALNALIQFRKQEGAILEKDLLSRVN
                     LILDYLQRVEPFEKQRIHDVRKRISDSLKQIDANIKVDDNRFEQEVIYYLEKLDITEE
                     KVRLRQHCSYFTETVNQPESVGRKLGFIAQEMGREINTLGSKANNAEIQKIVVMMKDE
                     LEKIKEQSLNIL"
     gene            4633..5205
                     /gene="gmk"
                     /locus_tag="PLW22_09910"
     CDS             4633..5205
                     /gene="gmk"
                     /locus_tag="PLW22_09910"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOU75057.1"
                     /translation="MEGKLLIFSAPSGAGKTTIVRHLLEKFPQLEFSVSACSRMPRDG
                     EVNGKDYYFLTVDEFRNRIEKGEFVEWEEVYPGSYYGTLWSEVKRIWDKGHHVMFDVD
                     VKGGVNLKRKFPNNSLAVFVMPPSIDELRERLLKRGTETEESLKVRIGKAQEEMTYAN
                     QFDYVLTNNQVETAFAEAERIVENFLLKKL"
     gene            5202..5804
                     /gene="nadD"
                     /locus_tag="PLW22_09915"
     CDS             5202..5804
                     /gene="nadD"
                     /locus_tag="PLW22_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOU75058.1"
                     /translation="MKVGLFFGSFNPIHTGHLVIAEYILEFFDLSEIWFVVSPQNPLK
                     SPDDLEKDEHRLNMARLAVHENNKRLKVCDIEMSMPRPSYTIDTLKKLGELYPENEFA
                     LIIGSDTLQTLPQWKSYKELVSSYDFLVYPRECNAYKQDFPTERFRLVDAPIIGISST
                     HIRSIIAKGKDVLGYVPDDVWDYIERHGLYGYKGIKISNL"
     gene            5806..6063
                     /locus_tag="PLW22_09920"
     CDS             5806..6063
                     /locus_tag="PLW22_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU75059.1"
                     /translation="MKEFEDLLQEGRLAEAEELLLTLDQADDIVLYSWGRLYSRKGEE
                     AKAISYYAKALEINPNNEEARVRLEIAREIFSFRDPNLYNH"
     gene            complement(6060..7400)
                     /locus_tag="PLW22_09925"
     CDS             complement(6060..7400)
                     /locus_tag="PLW22_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU75060.1"
                     /translation="MMRSKTKNRLQTISILLLFITSSFSASHAQSADTLKISDAIKIG
                     LENNFSLRIARNEELVARNNNTLGAAGFFPKVDISSSASGSNYNIRQELATDEVNDTK
                     NYQTTSVYGGVQLSWTLFDGFAMFANRQRLSTLEGINSIAYQLKVEDVVSDIIVNYYT
                     LSAEKQLLDIYKEILRLSADRLNIAREKAAIGTSYLLAVMQAEVDYRTDSTQVLRQIN
                     RIKDLTITLNKLLGRDPSIQFEIESKIPEVKLYNQDSVITMLKSNNKELLISRLNLRL
                     KQIAIKEAQASRYPRLTLSSAYNFSQSNTPDGSTTVSRNIGPSYGVTLGVPLFNGFNV
                     NRNITNARIYMENQEFNIQALENDLTAEAVRLTNNLMLATNLVEIEKKSALLAKQNSD
                     ISIEKYRVGLISDLELRDAQIRYLNAEFRYQNALIQAKSAEIAIQVLIGNLQMP"
     gene            complement(7387..>8294)
                     /locus_tag="PLW22_09930"
     CDS             complement(7387..>8294)
                     /locus_tag="PLW22_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU75061.1"
                     /translation="TVRDVADALQLYFSGQRYGFFIRNGKQYYVIGQADRPFRDDPDD
                     LKNIYVRSTKGNLVDLGSLIRTSEQSIPPQRYRYNRYISATFSASPAPGYTLGQGIDA
                     MREISKEVLDDSFSTTLTGISQQFEESSNSLYFAFLLALILVYLVLAAQFESFRDPLI
                     IMFTVPLALAGAVLSLWMLGHTLNIFSQIGIIVLVGIVTKNGILIVEFANQKKIAGMN
                     KFEAVTFAAKQRLRPILMTSLATIFGTLPIALALGAASTSRIPMGVTIIGGLLFALIL
                     TLYVIPALYTYVSTKKPNVIRHDEE"
BASE COUNT         2324 a         1616 c         1830 g         2424 t
ORIGIN      
        1 aagtgacccc ggagggatta gttccgcttc gcttcatgat ccctccggca atgcttgcaa
       61 gggtttcttt accaaataaa gaaagccagt ctgtattgac tggcttatca agtgaccccg
      121 gagggattca aacccccaac cttctgatcc gtagtcagat gctctattca gttgagctac
      181 ggagtcgttt gttttgcgtg tgcaaagata gatataattt tttttcctgc aaagggtggt
      241 caaaaaaaaa atccccaaac ggggattttt ttacatattt tcgccttcaa cgccacgttt
      301 cttttctttg atgcgtgctt ttttaccggt aaggttacgc aggtagtaaa tacgagccct
      361 acgcactcta ccttccttgg caacttcaat ttgttcaatg aaaggtgagt gtaaagggaa
      421 aatcctttct acgccaacac cccctgaaat tttacgaacg gtaaaggttt cagtagcgcc
      481 ggttccctta cgctgaatta caacacccct gaagttctga atacgctcct tgttaccttc
      541 cttaatcttg tagtgaacaa ccactgtgtc gccactcttg aaatcggggt gattcttttt
      601 ctcaacaaat gcttgttcgg caatcttaat gagatccatt ttatactgtt ttatttgatt
      661 aacactaagg tgtggaatat taccggctcc taagtcctga taagagatta cacaccaatt
      721 cggccgcaaa attatttact ttttgggaaa ctaccaaaaa tagaagcaat taattgaaaa
      781 attttaggta aaatccaatt ggattgtcct tactatatag tcgtatcgtt tcttctccgg
      841 taaaagtgtc ttgagttccc atgctcacca tatccaattt catcgccggt tagctgaata
      901 cgtgcttcga ggcaattctt gcaatcatcg gcagcatcgt cggtgcaggg tttgttctcg
      961 tatagcttgt aatcgttacg gtaggcaccg ggagttatgt ttggcataac gatattagct
     1021 cccactttta tggccttttc tctaccaaga gggtcaatag cctgaagtgc ggttggcgca
     1081 gcaatgttga tatccttcat gaggattcga agcaaggctg tcattttaag agccaggtta
     1141 aagcgttcgg taagcggaag aagtttatct cgatactggt aaagtggggt atcggggtgt
     1201 tcaacgtaag ggcccatgcc aaccatgtcc acatctattt cctgcatcca aagcaggtcg
     1261 tcggcaaggt gctctagggt ttgaaaaggc agcccaatca ttacccctgt tccaacctga
     1321 tatcctacac gtcggagggc cctcaggcat tccagccgtt cgtcaaaatc gtgatgctta
     1381 tcgttgggat gaagctttgc atagagttca cggttcgagg cctcaatgcg gagcaggtaa
     1441 cgaatggctc ctttgttaaa ccatcgccgg tatgtttcct cggtctgctc accaagcgaa
     1501 agtgtaattc ccagctcatg gtttgtggct ttgttgattt tatccagtag gttttctatt
     1561 ctatcaacaa actctttagt gcttacctcg ccagtttgaa tggcaatgct tccgtaacgg
     1621 ttttcccagg caaatttggc tgcctcaatt acctcatcgt cagttaaatt ataccgatga
     1681 acgttggtat tgccagaacg gatgccgcag tacaaacagt ttttacggca aatattcgac
     1741 atttcaatta gacctctgaa gtaaaccttg cgtccaatat acttagcctt tacctcagca
     1801 gcctttgtga aaagctttgt tttttcttcg ccctcgcagc ccaagaggta tacgagttct
     1861 tccttattta aattatcttt atttaaaaga ttatcgataa tcattgtata ttagtttgta
     1921 ttttttccta agtttaccaa ctaaaatggt ggttggtaat cctttgcaaa aataatttaa
     1981 ttcccatgga acgagctaag gcaataggtc aaataatagc tgttataaaa cagctcaaaa
     2041 aggaaacgga tgagctattt gcacctgaga atattattaa ccattcggaa ttgcttattg
     2101 aggagtattt ccagcaactt ttcaatccgt ttttaaaaaa ggttaaagcc gatattcgtt
     2161 ctttaccatt ggatgagtat ttacccattg agctggactc atggattaga atactaaaaa
     2221 tgaaactagt tgccattgag cgcggtgagc acaccgagaa gtatgccgag gtgctatata
     2281 tcaagcaaag gttttacccg gagctaattg atgttttaaa ggagttgcag cttgaggcac
     2341 ttgaagccga atttgatgct cgtgttgata gggagcaact atcaaagcca attcttggcc
     2401 ctaacaccat agttctctcc attgaatcgg tagctatgct tattaccatg ctatacaact
     2461 tcaatatcat ttcgggtgcg gttcccattg atgatatttg caggagtttt gccgcgatga
     2521 ccgggtataa tgcaggcgaa ctgaaacagc tgcttaggtt tgatgaggag aatggccgat
     2581 taacatcaat agtaaaagcc gaggatatgg atgtgctgct taagctattg cggagtattg
     2641 tggcaagaat cgagaactta cgtcagtata ccgattagtt atagggtatg catttgtagg
     2701 gcaggttgat atcgggtagt tcaaacagaa cttcaccctc atggagcagt tcctcatcct
     2761 cctcatggta gttccatata acctcaactt caaagtttgc gcttcgaaac gacgatattt
     2821 ttcttaaaac gtcgcggaga tacttgtatg aagtggaatt gatataccta aaattaaggt
     2881 caacaataat ttttgatggt tcctggtttt ttatgaactc atagtattca tcaagccaat
     2941 cgagtatttg ctgataaaat tccttggcat tttccgggtg cgaaataccg gaaatggaaa
     3001 atactttggt atcggggttg aagtatattt cgggtgaatc aatctcagcc tctattctta
     3061 aagctttcat tgcttctttt tcaatttaca acgtaaaatt aaacctttag cgttattaat
     3121 agcaaagttt taacaacatc tttgatgaaa ataaaaaata aatgggtgaa gctataaacc
     3181 ttttaggtaa aatattatca gtaataaaac actttttaac tttttgcggt tagtaaaatg
     3241 taattttggt tttatatttg tggtatacac attagcttcc ggtactttca tggagagttg
     3301 gttagttagt tcaaggaagc tttcgatttt cgagtaaaat cggagtgttt ggggcgaagg
     3361 aggtcaactt gacctccttc atttcattat ttacttcttg aaaatgaagt aaacagctaa
     3421 tatcagaaac ccaaatccta ttatgtggtt tgtccgaatt gtttcggttt taaaagcaaa
     3481 aagggtaaag cccataaata ctaccagtgt aatagcctcc tgaatcactt tgagctcaac
     3541 aagcgaaaag gggccaccat tctccctgaa acctaaacgg ttagcgggaa cctgaaaaaa
     3601 gtattcgaat agggctatgc cccagctaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnng tgatatttaa atatacttgc atgctagtat cgatgaccgg ctatggaaaa
     3781 gccgaaaggg tttttggaaa caaaaaaatt gttgttgagg ttcgttcact taactcaaag
     3841 cagttcgatt ttaacctccg cttgccagca atttaccgtg agctggagat ggagatcaga
     3901 actaaagccg ccaaggaatt agagcgcgga aaagttgata tgtccattag cattgaggag
     3961 actgccgagt cgcaggccat acccataaac gattccgttt ttatggctta ctaccggcag
     4021 ctaagcgata ttgcttcaaa aaatagcatt agcttcaacc ctgccgaggt tgttccggcc
     4081 attctccgat tgcctgaggt tattagggca cagaaggaag agattgcccc cgaagagctt
     4141 gacgcagttt tgcaggcatg cgctgaagcg ttgaatgcgt taattcagtt ccgaaagcag
     4201 gagggcgcaa ttcttgaaaa ggatttactt agccgtgtta acctgatact tgattacttg
     4261 caaagggttg aaccttttga aaagcagcgc attcacgatg ttcgtaaacg gatttcggat
     4321 agcttaaagc agattgatgc caatattaag gttgacgaca accgttttga gcaggaggtt
     4381 atttactacc ttgaaaagct tgacattacc gaggaaaaag tccgtttgcg acagcattgc
     4441 agctacttta cggaaacagt taaccagcct gaatcggttg gacgtaagct tgggtttata
     4501 gcccaggaaa tgggacgcga aataaacact ctgggttcaa aggcaaacaa tgccgaaatc
     4561 caaaaaattg tggtgatgat gaaggatgag cttgagaaaa tcaaggagca atcgttaaac
     4621 atcttgtagg taatggaagg caagctatta atattttcag ccccttcggg tgcaggtaaa
     4681 actaccattg taaggcattt gttggaaaaa tttcctcaac ttgaattttc ggtatcggct
     4741 tgcagccgaa tgccgcgcga cggagaggtt aacggtaagg attactattt ccttactgtt
     4801 gatgaattcc ggaataggat tgagaaaggc gaatttgtgg agtgggaaga ggtttatcca
     4861 gggtcatact acggaacgct gtggtctgaa gttaaacgta tttgggacaa aggacaccat
     4921 gtaatgttcg atgtagatgt taaaggaggt gttaacctca agcggaagtt tccgaataac
     4981 tcgttggcag tatttgtgat gcctccttcc attgacgagc tgcgtgaacg actacttaaa
     5041 aggggaacag aaaccgagga gtcgctaaag gtgcgtattg gtaaagcaca ggaggaaatg
     5101 acctatgcaa atcagttcga ttatgtatta accaataatc aggtagaaac tgcatttgcc
     5161 gaggctgagc gtatagttga aaatttttta ttgaaaaaat tatgaaagta ggactattct
     5221 ttgggtcgtt taacccaata cacacggggc atctggtaat agccgaatat atacttgagt
     5281 ttttcgattt atcggagata tggtttgtgg ttagcccaca aaatccgcta aaaagccctg
     5341 acgatttgga aaaggatgag cataggttaa acatggctcg tttagctgtg cacgagaaca
     5401 ataaaaggct taaggtttgc gatattgaaa tgtccatgcc tcgcccttca tacaccatcg
     5461 ataccctcaa aaagctgggc gaactttatc cggaaaatga gtttgctttg attattggct
     5521 ccgatacgct tcagaccctc ccacaatgga aaagctacaa ggaattggta tccagctatg
     5581 actttttggt gtatccccgt gaatgtaacg cttacaagca agattttcct actgaaaggt
     5641 ttagattggt tgatgcgcca attataggta tttcctccac tcacatacgt tcaattatag
     5701 caaaaggcaa agatgttttg gggtatgtac ccgatgatgt atgggactac attgagcggc
     5761 atggactata cggttacaag gggataaaga tttcaaattt atagaatgaa agagtttgag
     5821 gatttgcttc aggaaggaag gcttgctgag gccgaggagt tacttttaac actggatcaa
     5881 gctgacgata tcgttttgta tagctggggc cgcttgtatt ccagaaaagg cgaggaagca
     5941 aaggccattt cgtactacgc taaagcatta gagattaacc ccaataatga ggaggcccgg
     6001 gtaagactcg agattgcaag agaaatattc tcgtttcgcg atccaaacct atacaaccat
     6061 tagggcatct gcaaattccc tattaggacc tgaattgcaa tttcagccga tttggcctgt
     6121 attaaggcat tttgatacct aaactcagca ttaaggtaac ggatttgggc gtcgcgtagc
     6181 tcaaggtcac ttattagccc aacacgatac ttctcaatgg agatatcgga gttttgcttt
     6241 gccagtagtg ccgatttttt ttcaatctct accaggtttg ttgccagcat cagattattc
     6301 gtaagtctta cggcttcggc agttaaatca ttctccaaag cctgtatatt aaattcctga
     6361 ttctccatgt agatgcgagc attggtaatg ttcctgttta cattgaaccc gttgaatagt
     6421 ggaacgccaa gtgtaactcc gtacgaaggt cctatgttcc gggaaacggt ggttgaccca
     6481 tcaggtgtgt tgctttgact aaaattataa gcggatgaga gagtaagacg agggtatcgt
     6541 gaggcttgag cttccttaat ggcaatttgc tttaaacgta aatttaaccg tgatataagc
     6601 aactccttgt tgttgctttt gagcatggta attactgaat cctgattgta aagctttact
     6661 tcagggattt tactttcaat ttcaaattga attgatggat cacgcccaag gagtttgttc
     6721 agggtaatgg taaggtcttt aatcctgtta atttgccgta acacttgagt tgaatcggtt
     6781 ctgtaatcaa cctcagcttg cataacggca agcaggtagc tggtaccaat ggctgctttt
     6841 tcgcgggcaa tattaagcct atcggctgaa aggcgcaaaa tctctttata aatatcgagc
     6901 agctgctttt cggccgaaag ggtatagtag ttaacaataa tatccgatac tacatcctca
     6961 accttaagct gataagcgat gctgtttatt ccctccagtg tacttagtcg ctgccggttg
     7021 gcaaacatgg caaaaccatc aaagagtgtc catgatagct gaacccctcc ataaactgaa
     7081 gtggtttgat agttttttgt atcgttaacc tcgtcggttg ccagttcctg gcgtatatta
     7141 tagttgcttc cgctggccga ggaactaata tctactttag gaaagaatcc tgctgcccct
     7201 aaggtgttgt tgtttcgagc aactagttcc tcgtttcggg ctattctaag ggagaagttg
     7261 ttctccaacc caatcttaat ggcatcggaa attttgagcg tatcagctga ttgtgcatgt
     7321 gaggctgaaa atgagcttgt aataaacaaa agcaatatcg atatagtctg taatctattt
     7381 tttgttttac tcctcatcat ggcggataac atttggtttt ttggttgaaa cataggtgta
     7441 aagtgctggt attacgtata gcgttagaat aagagcaaag agtaatcctc caataatggt
     7501 aacccccatt gggatgcggc ttgttgatgc ggctcctaat gctaaggcaa tgggcaatgt
     7561 tccgaaaatg gtggcaaggc tggtcatcag aatgggacgt agcctctgct tagcggcaaa
     7621 ggtaactgct tcaaatttat tcatgcccgc aatctttttc tggttggcga attcaacaat
     7681 cagaatacca tttttggtaa caattccaac cagcacaatt attccaatct ggctgaaaat
     7741 gttaagggtg tgtcctaaca tccacaacga cagaactgcc ccagccaaag cgagtggaac
     7801 tgtaaacata atgataagtg ggtcacgaaa actttcgaac tgtgctgcta aaaccaggta
     7861 aacaagaatt agcgcaagca gaaatgcaaa gtataggctg ttggagcttt cctcaaactg
     7921 ctgcgagata ccggttaaag tagtggaaaa actatcgtcg agtacttcct tggaaatctc
     7981 gcgcatggca tcgatgcctt gccctaatgt atacccaggt gcaggcgatg cgctgaatgt
     8041 agccgaaatg taacggttat acctgtaacg ctgtggagga atactctgct cgctagttct
     8101 gattagcgaa cctaaatcga caaggtttcc ctttgtgctc cgcacgtata tgttttttaa
     8161 gtcatcggga tcatctcgga atggtctgtc ggcttggcct attacgtagt actgcttccc
     8221 gttccgaata aagaaaccgt agcgctgtcc gctgaagtaa agctgaaggg catcggcaac
     8281 atcccttacg gtaa
//
LOCUS       DAOOWJ010000113         8274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_8606_length_8274_cov_7.385327, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000113 DAOOWJ010000000
VERSION     DAOOWJ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8606_length_8274_cov_7.385327"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..969
                     /locus_tag="PLW22_09935"
     CDS             52..969
                     /locus_tag="PLW22_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75062.1"
                     /translation="MQMFRYLKLIVLNRNAILVFAVIAGIIVGEHAGSLKFLTFPALA
                     LTMTFSMTGMSLVQLRSEPHIFKPMLTGIALNYVAFSAVMLPLAYWLMPTKELFYGFV
                     IIAATPPGVAIIPFSFILRGNVVYAIISVTGAFLGSILIAPLMVKLFATSSGINPTDL
                     LLLMLQLVVAPLVVSRFLLWKPIKPFIEKVRGKVVDWGFAILIFVAVGLNRQVFFSSP
                     ALLLSITGILVVTIFGLGHFTNILARHLNISQEKRIPMVMLIGIKSSGFSVFTALTLF
                     GKEAAIPSAVMAVVVLLYLIFLSIKSKAL"
     gene            970..1731
                     /locus_tag="PLW22_09940"
     CDS             970..1731
                     /locus_tag="PLW22_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU75063.1"
                     /translation="MKITFLGTGTSQGMPVIACPCPVCQSTDPRDWRLRTSALIEHQG
                     INIAIDAGPDFRQQMLRAKVKTLQAILLTHEHRDHISGLDDVRAYNWLQKRPMDIWGE
                     ARVLNELKREYSYVFAEHKYPGIPEFELHSIDGQPFEVMGINIIPIRVYHYKLPIYGF
                     RFGDLTYITDANFIPEDEKEKIIGSKYLVINALRTQKHISHFSLPEALELIAQLSPRK
                     AFITHIGHQMGFHNEVAKTLPENVCLAYDGLELEF"
     gene            complement(1728..2909)
                     /locus_tag="PLW22_09945"
     CDS             complement(1728..2909)
                     /locus_tag="PLW22_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU75064.1"
                     /translation="MKNNILNHNLIRLYILKVSHWFMLVMPIVVLFYKENGLNVSQVF
                     ILQSVYSLSIVLLEIPSGYFADALGRKNTIFIGAVLGFIGFAIYSISYGFWGFLMAEV
                     VLGFGQSMISGADSALLYDSLVDAKQQDRYIKHEGRMTSIGNFAEASAGILGGLLAAV
                     SIRYPYYGQTLVALLAVPAAFTLIEPKTEHNRLELGWKQIVDVVRFALVKNLRLRWNI
                     LLSSVVGASTLTMAWFVQPWLIRADTPVEAYGAIWTVLNLLVGVAAMFAYRVELKLGR
                     NRTTGVLILFLFLGFVMAGFIRSYWAMPFIVIFYLARGVATPILKDSINRIAPAPMRA
                     TILSVRNFIIRIIFSVWGPFYGWLTDRWSLKVALITAGFIFIILSTLTFIALFKVERE
                     N"
     gene            complement(2991..4520)
                     /locus_tag="PLW22_09950"
     CDS             complement(2991..4520)
                     /locus_tag="PLW22_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOU75065.1"
                     /translation="MMHKEFIYHPRDFRKIELWKNVKDEEWYNPQWQLKNSIRSVDQL
                     KHVIKLNDYQEQEIRRVVDELERQGKDPLRITPYYASLMDEDPFHPDIPLEEKGNKRL
                     DPIFWQSVPTPANLLFPNTGREASMNEGSRSYGAAYQRYPNRVALFVAQNTSCASYCV
                     HCQRAKSLDAMCDVNIELINKGLFYINFNKNIDEVLVTGGDALRVSKKRLQYVLEELS
                     KMEHIRAIRIATRVPVVLPMAVTDEILTLIKESANRYTKGQEKYVYFMTHVNHYKEIT
                     ADFAAAIKRINSYGFTVRKQTVFLNHVNDYFVTLAKTFRRMFWVGVHPYYLLQCHKEK
                     GIVHFISPIQVGQIYMKHMQGWLSGIVRPNYAVNIEGGGGKVLLMPLNYDNLNIDLKI
                     EDKISESKAKVYTWDDKEIFDYEALGRATPDEYNKALRIMDKFIGRKGVFVPKIILID
                     NNGNYIETTNRTKLPTFEKQKKAVLLGYETNQSGMPITNPAKISKKLDELYQKSTFSK
                     K"
     gene            complement(4578..5828)
                     /locus_tag="PLW22_09955"
     CDS             complement(4578..5828)
                     /locus_tag="PLW22_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU75066.1"
                     /translation="MNRFFLLILSLNLLVVISGCKKPKRYDLIVENATISTDSGLVDG
                     KTILVMGDTIAGIIDAGEPVRTRKVVDADGGIVMPGFIDSHTSIACFFKSNGKAFDTH
                     PKSLTTFYRSIVSKHFLPYGVTTIFDAEPDSAWVPQIVSWKPNPKLTDVMVARSVEMG
                     KSACNPSQSPYVFIKGIYDSKNQEHAGDNCFWILTGVQNGLNYPKNIEGIQSIMNSLL
                     LLTGSSDKVLNRIANIYGIRQNIPDELLAIEGISYLAENSPVLLDSVAAILGSNNASV
                     STGLYRIKHFVDSGFTTCGIEPTEAIKQRLKYGFAHLMEYVKALHTKGVELRIGYNMP
                     FDGSAFAKEQNLLLESGFPVNEIGKISSFNSAKAFAIHGKKGKIAIGYTADLVVFLKN
                     DAGNSFDFTKVKRVIKGGRAEKMK"
     gene            complement(5958..8054)
                     /locus_tag="PLW22_09960"
     CDS             complement(5958..8054)
                     /locus_tag="PLW22_09960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOU75067.1"
                     /translation="MRKILFLMPLAAIVCGFALVKTENPFFTEYKTPFKVPPFNEIKL
                     EHYVPAFEKGIEEQQKEIDAIVNNPETPNFKNTILALDKSGRLLTKVSTVFYSLNSAE
                     TSPEMQAIARQVAPKVTAHRDNISLNDKLFKRIKAVYDNRMNLKLDSLQLRTVERYYD
                     DFVRNGANLNDDDKAKLREINQKLSQLGLKFGENLLDETNKNFILVIDKPEDLEGLSK
                     DIIDAAAITAKQFGHEGKWVFTLQKPSMIPFLQYSKKRELREKLYMGYVMRCNNNDKF
                     DNKAILLEIANLRAQKAKLLGYETFNHYVISNNMAKTPDAVYSFLNQVMEPALNAAIR
                     DRDAMQAIIDREGGNFKLAPWDWWYYAEKLKKEKYDLDEAELKPYFKLENVRDGMFYV
                     ANKLYGITFTKRTDLPIYHPEVEAFEVKDSKGQHVGLLYLDYHPRPGKRVGAWCGRFR
                     QQTYENGKKVAPIVNIVTNFTRPTETTPALLTWDEVETLFHEFGHALHGLFTDGPYDR
                     IAGSVPRDMVELPSQIMEHWAGEPQVLKFYAKHYQTGEVMPDALIEKLQRSSKFNQGF
                     ATVEYIAAAILDLDWHSFTEPKQVDVLEFENQSMSRIKLIPEIYPRYRTSYFSHIVGG
                     YASGYYVYLWAEVLDSDAFNAFVETGDIFNKDVAAKFRKYVLAEGGNNDGMVQYLKFR
                     GKAPSIDPLLRNRGFK"
BASE COUNT         2394 a         1791 c         1649 g         2440 t
ORIGIN      
        1 ccattttttc aatccttcta tctatattta ctaaaatttt ttacgaaggt aatgcaaatg
       61 ttccgatatc taaagcttat agtattgaac cggaatgcaa tattggtttt tgctgtaatt
      121 gcagggatta ttgtgggtga gcatgcaggt tccttgaagt ttctgacatt ccctgcacta
      181 gcattaacca tgacattctc catgacgggc atgagccttg tgcagctaag gagcgaacca
      241 catatattca aaccaatgct aaccggtatc gctttaaatt acgttgcatt ctcagctgtt
      301 atgctaccac tggcatactg gctgatgcct accaaagaac ttttttacgg atttgtaatc
      361 attgcagcca cccctccggg tgtagccata atccctttct catttattct gagaggtaac
      421 gtggtttacg ccattatttc ggttacagga gcatttctgg gttctatcct aattgcacct
      481 ttaatggtaa aactctttgc tacctcatcg ggaatcaacc ctactgactt acttttgctg
      541 atgcttcagc ttgtggttgc tccgttggta gtgtcacgct ttttgctttg gaaacccatt
      601 aagccattta ttgaaaaggt tagaggtaaa gttgtggact gggggtttgc aatactaatt
      661 tttgtggctg tagggttaaa caggcaggtt ttcttttctt cccctgctct actacttagc
      721 attacaggta ttttggttgt tactatcttt ggacttgggc attttaccaa tatcctggca
      781 cgacacctga atatctctca agaaaaaaga ataccaatgg taatgcttat tgggattaag
      841 agctctggtt tttcggtttt cactgccctt accctttttg gtaaagaagc ggcaatacct
      901 tccgctgtga tggcagtggt tgttttgctc tatttgatat ttttgtcaat caaatcaaaa
      961 gcattgtagg tgaagataac ttttcttggc acaggaactt cgcaaggtat gcccgttatt
     1021 gcatgcccat gtccggtttg ccaatcaacc gacccacgcg actggcgact tagaacatct
     1081 gcactcattg aacatcaagg aataaacata gcaattgatg ctggccccga tttccggcag
     1141 caaatgctcc gggccaaggt aaaaaccctt caggccatac tgcttaccca tgagcaccgc
     1201 gaccatattt ccggccttga cgatgttagg gcctacaact ggcttcagaa acgccccatg
     1261 gatatttggg gcgaagcaag ggtgcttaat gagctgaaaa gggaatatag ctatgttttt
     1321 gctgagcata aatacccagg aatccctgaa tttgaactgc atagcatcga tggtcaacca
     1381 tttgaggtga tgggcattaa catcatccca ataagggttt accactataa gttacccata
     1441 tatgggttta gatttggcga tttaacatac attaccgatg caaactttat tcccgaggat
     1501 gagaaggaaa agattattgg ctcaaagtat ttggtaataa atgcactaag gacgcaaaaa
     1561 cacatctcgc acttttcact gcctgaggct cttgaattga ttgctcagct cagcccccga
     1621 aaagctttca tcacacacat tggccatcaa atgggatttc acaacgaagt agcgaaaaca
     1681 ttacccgaaa atgtatgcct tgcctacgat gggcttgagc tagagtttta attttccctt
     1741 tcaactttga aaagtgcaat aaatgtaagg gttgagagga taataaagat aaatcctgcg
     1801 gttattaagg ctacttttaa actccaccta tcggtaagcc aaccgtaaaa gggcccccaa
     1861 accgaaaata ttatccgaat aataaaattt cgaaccgata gtattgttgc cctcattgga
     1921 gcaggagcaa ttctgttaat gctatctttt agtatagggg tagctactcc cctagccagg
     1981 tagaaaatca caataaatgg cattgcccag taactcctaa taaaacccgc cattacaaag
     2041 ccaagaaata gaaaaagaat taaaactcca gtggttctat tccttcccaa tttgagttca
     2101 accctatagg caaacatggc agcaacaccc actagcagat ttagcactgt ccaaatggca
     2161 ccgtaggcct ccactggcgt atcagcacgt atcagccacg gttgaacaaa ccaggccatg
     2221 gttaaagttg atgctcccac caccgatgaa agcaatatgt tccagcgtaa gcgcaagttc
     2281 ttaacgagag caaaccttac aacatcaact atttgcttcc agcccagttc caatcggtta
     2341 tgctcggttt tgggttcaat aagcgtaaag gcagccggaa cagctaaaag ggcaacaagg
     2401 gtttgaccgt agtatgggta acgtatgcta acggccgcaa gcaatccgcc aagaatacct
     2461 gctgaagctt cagcaaaatt acctattgag gtcatcctac cttcgtgctt tatataccta
     2521 tcttgctgct tagcgtcaac aaggctgtcg taaagcagag ccgaatcggc acccgaaatc
     2581 atactttgcc caaaaccaag cacaacctca gccattaaga atccccaaaa accataggaa
     2641 attgaataga ttgcaaagcc aataaagccc aaaaccgccc caatgaaaat ggtgtttttt
     2701 cgccctagtg catcggcaaa gtaacccgat gggatttcga gcaatacaat ggaaagtgaa
     2761 taaacacttt gaaggataaa tacctgactt acgttaagcc cattctcctt gtaaaataaa
     2821 acaactatgg gcataaccag cataaaccaa tgggagacct tgagaatgta aaggcggatt
     2881 agattatggt ttaatatgtt attcttcatt gcagtaaaaa tgcaaatata aagaaaagag
     2941 gccatgctga aaattcagca tgacctatag tgcggaggaa actatcagat ttattttttt
     3001 gaaaaagtag atttttgata aagctcatcg agctttttac taatctttgc aggatttgtt
     3061 ataggcatac ctgattggtt agtctcataa ccaagcagta cagccttttt ttgcttttca
     3121 aatgttggaa gtttagtgcg gttggttgtt tctatgtaat ttccattgtt atcaatgaga
     3181 attattttag gtacaaatac ccccttccta ccaataaact tatccataat tcttaatgct
     3241 ttgttatact catcgggtgt tgcacgtcct agtgcctcgt aatcaaatat ttccttatca
     3301 tcccaggtgt aaacctttgc tttactctcc gaaattttat cttcaatttt caggtcaata
     3361 tttagattat catagtttaa gggcattaat aggactttac cgcctcctcc ttcaatattt
     3421 acagcataat ttggtctaac aatacccgat aaccatccct gcatgtgttt catataaatt
     3481 tgaccaacct gtatgggtga tataaaatga acaatccctt tctctttatg gcattgtagc
     3541 aggtaatatg gatgaacccc aacccaaaac atacggcgaa aggttttggc aagggtaaca
     3601 aaatagtcgt taacatgatt caagaaaaca gtttgtttac gaacagtaaa accataacta
     3661 tttatacgct taatggccgc tgcaaaatca gcagtaattt ctttatagtg atttacatgg
     3721 gtcatgaagt aaacgtattt ctcttgaccc tttgtataac gattggccga ttctttaatt
     3781 aatgttagaa tctcgtcagt tacagccata ggtaaaacca ctggaactct tgtagcaatt
     3841 cttatggcac gaatatgttc cattttactc aattcctcta aaacgtattg caagcgtttc
     3901 ttgcttaccc gcaaagcatc gccaccagta accaatacct catcaatatt tttattgaag
     3961 ttgatataaa ataagccctt gttaataagt tcaatattca catcacacat agcatcaagc
     4021 gattttgccc tttggcaatg aacacaatag gatgcgcaac ttgtgttttg cgcaacaaaa
     4081 agtgccactc tattggggta acgctgatag gctgcaccgt aacttcttga cccctcattc
     4141 attgatgcct ctctcccggt atttggaaat aaaagattgg ctggtgtagg aacactttgc
     4201 caaaaaattg gatcgagcct tttgtttcct ttttcctcta aaggaatatc gggatgaaat
     4261 ggatcttcat ccattagcga agcgtagtaa ggagtaattc ggagcggatc cttcccctgg
     4321 cgttccagct catcaacaac acgtcgtatc tcttgctcct gataatcgtt taatttaatg
     4381 acatgtttta gctgatcaac actccggatt gaatttttta gctgccattg aggattgtac
     4441 cactcctcat ctttaacatt cttccaaagt tctatttttc gaaaatctct tgggtggtaa
     4501 atgaattcct tgtgcatcat tgtttgttta aatttattgt taattatact taagtcaatc
     4561 acaattggaa attaccctta cttcattttt tcagctcttc cgcctttaat tactctcttt
     4621 accttagtaa aatcgaaact atttccagcg tcatttttta ggaataccac caggtcggca
     4681 gtatagccta tggctatttt accttttttg ccatgaatgg caaaagcctt agcagaatta
     4741 aacgatgata ttttaccaat ttcgttaact ggaaaacccg actctagtag aaggttctgt
     4801 tcctttgcaa aggctgagcc gtcaaatggc atgttatagc caatgcgtag ctcaaccccc
     4861 tttgtatgca gggcttttac atattccata aggtgagcaa atccatactt taagcgttgc
     4921 ttaattgctt cggttggctc aataccgcaa gtggtaaagc ccgaatctac aaaatgtttt
     4981 atccggtaaa gccctgtgga aaccgaagca ttgtttgacc caagaatagc ggcaacggaa
     5041 tcgagcaaga cagggctatt ttcagccaaa tacgatatcc cctcaatggc caacagctca
     5101 tcgggtatat tttgcctaat gccataaata ttcgcaatcc tatttaacac tttatcggat
     5161 gagccagtta acaggagtaa cgaattcata attgattgta tcccctcgat atttttagga
     5221 tagttcaaac cattttgaac tccagtaaga atccaaaagc aattatcccc cgcgtgttcc
     5281 tggtttttgc tatcgtaaat acccttaata aaaacgtagg gtgattggct gggattacat
     5341 gcagattttc ccatttcaac cgaccgtgca accataacat cggtaagttt tgggtttggc
     5401 ttccagctta cgatttgagg aacccaagcg gaatcgggtt cagcatcaaa aatagtggtt
     5461 accccatagg gtaaaaaatg cttggataca atgcttcggt aaaaggttgt caggctttta
     5521 gggtgagtat caaaagcttt gccgttcgac ttgaaaaaac aggcaattga tgtatggcta
     5581 tcaataaaac cgggcataac tatgccacca tcggcatcaa ccaccttgcg ggtgcgaaca
     5641 ggctcgccgg catctattat tccagcaatg gtatcaccca tcactaaaat ggtttttcca
     5701 tcaaccaaac cgctatcggt agaaatggtg gcgttttcca caatcaaatc gtatcgtttg
     5761 ggctttttac aaccgcttat aacaactaaa aggttaaggc taagaataag taaaaaaaat
     5821 cgattcatag ctttacaaag attacatggg ttagaaacac tgataaacgg gcaaacactt
     5881 taaacacctt gtaaatataa gaataaacgc ttaaagaaaa aaaattaggg gttgcgtttg
     5941 caacccctat tcatattcta cttaaatccc cggtttcgta aaagcggatc aattgatggt
     6001 gctttaccac ggaatttcag atattgaacc ataccatcgt tgttaccccc ctcggcaagc
     6061 acatacttac ggaacttggc agcaacatcc ttgttgaaga tatcgcccgt ttcaacaaat
     6121 gcgttaaagg catcggaatc gagcacctcg gcccataggt aaacgtaata ccccgatgcg
     6181 tatccgccaa caatatggct aaagtatgag gtacggtaac gggggtatat ctctgggatt
     6241 agcttgatac ggctcatcga ttggttctca aactcaagca catcaacctg tttaggctca
     6301 gtgaaactat gccaatccaa atcgagaatg gccgctgcaa tgtactccac ggtagcaaaa
     6361 ccctggttga attttgagct gcgttgcaac ttttcaatca gcgcatcggg cataacctcg
     6421 ccggtttggt aatgcttagc gtagaacttc aggacttgtg gctcacctgc ccagtgttcc
     6481 ataatttgcg agggaagctc aaccatatcg cgtggaacac tgccagctat tctatcgtaa
     6541 ggcccatcgg tgaacaggcc gtgcagggca tgcccaaact catggaacag ggtctcaacc
     6601 tcgtcccagg taagtagagc tggcgtggtt tccgtaggac gtgtaaagtt tgttacaata
     6661 tttactattg gggcaacctt tttgccgttc tcataggttt gctgacggaa acgcccacac
     6721 catgcaccca cgcgtttacc cggacgtgga tggtaatcga ggtatagtaa tcccacgtgt
     6781 tgacccttac tatccttaac ctcaaaagcc tcaacctcag gatggtaaat gggcaaatcg
     6841 gtacgtttgg tgaatgttat accgtaaagc ttgttggcaa cgtagaacat accatcgcga
     6901 acattctcga gcttaaagta gggtttaagc tcagcctcat ccaaatcgta cttttccttt
     6961 tttagcttct cggcatagta ccaccaatcc caaggagcaa gcttgaagtt gccaccctcg
     7021 cggtcaatta ttgcctgcat ggcatcccta tcgcgaatgg cagcattcaa tgctggttcc
     7081 attacctggt tcaggaagct gtaaacagca tcgggtgtct tggccatatt attgctaata
     7141 acataatggt taaaggtttc atagcctaac agctttgctt tttgagccct taggtttgca
     7201 atctcaagta gtatggcctt gttatcgaac ttatcgttgt tattgcagcg cattacatag
     7261 cccatgtaaa gtttttccct aagctcacgt tttttggagt actgtaggaa cggtatcatg
     7321 ctgggttttt gaagggtgaa cacccacttc ccctcatggc caaactgctt ggcggtaatg
     7381 gcagctgcat caataatatc ctttgaaagc ccctcaagat cttcgggttt atcgataacc
     7441 agaatgaaat tcttgttggt ctcgtcgagc agattctccc caaatttcaa tcccagctgc
     7501 gaaagcttct ggtttatctc gcgcagcttg gctttatcgt cgtcgttcag gtttgcacca
     7561 ttacgaacaa aatcgtcgta atacctttca acggttcgta gctgaaggct gtcgagctta
     7621 aggttcatac ggttatcgta aaccgccttt attctcttga acagcttgtc gtttaggctt
     7681 atattgtcgc gatgtgccgt aacctttggg gcaacctggc gggctatagc ctgcatctca
     7741 ggcgatgttt cggcactgtt taagctgtaa aaaaccgtgc tcacctttgt tagcaaccta
     7801 cccgacttgt ccagagcaag aatggtattt ttaaaattgg gtgtttcagg attgttaaca
     7861 atggcgtcaa tctccttttg ctgctcttca ataccctttt caaaagcggg aacgtagtgc
     7921 tccagcttta tctcgttaaa aggcggtacc ttaaatggtg ttttatactc ggtaaagaat
     7981 gggttttcgg ttttaaccaa cgcaaacccg caaactattg ctgctaatgg cataaggaaa
     8041 agtatttttc tcatagtttt agtttttggt tttataatct ttggatgaga aatagaaagt
     8101 aaggtttaat aaggataaag gttaaaggat aaagggtaaa ggataaaggc tgaagactga
     8161 aagtgaaaag tgaaaagtga aaagcgaaaa gtgaaaagcg aaaagtgaaa ggataagata
     8221 tgtctagtcc tgaaataagt ctacaggtta gaaatagatt taagcgacac acat
//
LOCUS       DAOOWJ010000114         8135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_8807_length_8135_cov_8.215347, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000114 DAOOWJ010000000
VERSION     DAOOWJ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8807_length_8135_cov_8.215347"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1106
                     /locus_tag="PLW22_09965"
     CDS             279..1106
                     /locus_tag="PLW22_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU75068.1"
                     /translation="MTKALNKLSGKVVVITGASSGIGKALTYEFSKYNTKLVIGARNI
                     DELKAIASELSSVGVEVLPVKTDVTSESDCKNLVEQAISRYGQIDILINNAGVSMRAL
                     FKDVDLKVIRHLMDVNFWGTVYCTKYALPYLLETKGSVVGVSSIAGFHGLPGRCGYSA
                     SKFAMHGFLETLRIENLKNGLHVMIAAPGFTATNVRFSALTADGTPQGMSPRDEKNMM
                     TPEEVAKIIVNGIAKRRRNIIMGTEGKISVLAQRILPTVIDRLLYGYMAKEPNSPFK"
     gene            1157..2632
                     /gene="cls"
                     /locus_tag="PLW22_09970"
     CDS             1157..2632
                     /gene="cls"
                     /locus_tag="PLW22_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOU75069.1"
                     /translation="MVALNILTETFLNHLTTLLWAIYIITVLSTAAMIIHEKRDPAKT
                     STWVLLLITLPILGLLLYIVFGQNRRKEKLFSRKEIQDIEQIEYLSHKQVIRFSSKTD
                     IHHKLADHLSIITLLLNNSKALLTEKNEVEIFQTGNQAFDSIIESLYSATSSIHIEFY
                     IINDDKLGNRIKNILITKAKEGVKVRVLFDDVGSWSLPKRYVKELKDAGIEIYPFMEV
                     RFPLLTSRVNYRNHRKVVVVDGKVGYLGGMNIADRYIEGTRRLGQWYDTMLKIEGEAV
                     HSLQVIFLIDWYFVTGKIIRERNIYFPQPTVTSYHPLQIVTSGPDSDWASIMQAFFHA
                     ITRANKHIYISTPYFIPNESILTALKTAALSGIDVRIILPGKSDSTVVYWSSLSYVSE
                     LLEAGIKVYLYQNGFNHSKVMMIDGKLAMVGSANMDIRSFEDNFEVAAFIYDNQVTER
                     LEENFLSNLVQSQVITPQSWSKRPLKNSYKEALARLISPLF"
     gene            2689..3336
                     /locus_tag="PLW22_09975"
     CDS             2689..3336
                     /locus_tag="PLW22_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU75070.1"
                     /translation="MKRTLFILALTSLSLFSNAQFFRMGLKGGISSSNVKFSKATITD
                     GANQYIVEQGDAKLGFHAGLFARVQIMGFFVQPELLFSRSQGEVMLKDVTLNDVYNEM
                     QKFNKFDIPVIVGWKFGPARVGLGPVATILISENDGLKDKLADLTNQTVKNNFRSATF
                     GYQVGVGLDILKTLTLDLKYEGNLSKLGTGITLGGTDYSFDQRNPQIIMSVGFFF"
     gene            3549..4946
                     /locus_tag="PLW22_09980"
     CDS             3549..4946
                     /locus_tag="PLW22_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU75071.1"
                     /translation="MALVSTATTYFITHNYHKRKLSANAVNSSSHSVLLEQLREEIQK
                     LKIQCKELQIENDEQADTLAKAHLEIQRLEAAVQNAKIRAEEADILKSNFLANMSHEI
                     RTPMNGILGFAQLLKNEESQEKRDRYIDIIYHNGMMLVNLIDDIIDLSKIDAGQLTVN
                     PADCNLDNLMFEVYTFFNEIKFKQEKEHINIRLLNLNDDESNILYTDAQRLRQVLVNL
                     IGNALKFTERGSVEFGYLINENKSNIEFFVRDTGIGIPKDKQDIIFDRFRQVQEGSTR
                     KYGGTGIGLYISKHIIELLGGDIWFESEVGQGTTFHFSLPYHESKSVNDGSVIFKAKN
                     PEYKWEGITILIAEDIDTNYKYLSTVLADTKAKILWARNGEEAVSMVLNGSPVDIVLM
                     DIQMPVVDGYEATLRIRENYPDLPIIAQTAYALPHDNLKCFEVGCNDYVSKPINAHLL
                     KKKINDLLNVHVKSS"
     gene            complement(4933..5739)
                     /gene="kdsA"
                     /locus_tag="PLW22_09985"
     CDS             complement(4933..5739)
                     /gene="kdsA"
                     /locus_tag="PLW22_09985"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOU75072.1"
                     /translation="MKGIKHIESGNFFLMAGPCVVESRDNVMRIAETVSSITDKLKIP
                     YIFKASYRKANRSRIDSFAGIGDEKALKILGEVNERFGLPIVTDIHSAPEAAMAAEYV
                     DILQIPAFLCRQTDLLIAAAQTGKFVNIKKGQFVSPDSMKFAAQKVVDSGNTNVILTD
                     RGTMFGYHDLIVDFRAIPTMRAFGFPVVVDVTHSLQQPNQTTGVTGGRPDMIETIARA
                     AIATGADGLFMETHFNPAEALSDGANMLNIKYLEGLLERLVAIRQVVKNF"
     gene            complement(5768..7048)
                     /locus_tag="PLW22_09990"
     CDS             complement(5768..7048)
                     /locus_tag="PLW22_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOU75073.1"
                     /translation="MPTIPLAERMRPRNLNDYVGQEHLVGSKGVIREMVTRGTITSFI
                     LWGPPGVGKTTLARVVANELNRPFYTLSAISSGVKDVRDVIEKAKSQKFFNSPSPILF
                     IDEIHRFSKSQQDSLLGAVEQGVFTLIGATTENPSFEVITPLLSRCQVYILKPLSNEE
                     LELLFVRATTTDPELKKFRFELRERDALYRFSGGDARKLYNIIELITSTQNTNNQVVI
                     TNKAVVDVLQQNLAIYDKNGEQHYDIISAFIKSVRGSDPNAAVYWLARMIEGGEDPEF
                     IARRLVILAAEDIGLANPNALLMATSCFQAVQMIGMPESRIILSEATIYLATSPKSNS
                     AYEAIGKAQGIVKETGDLPVPLHLRNAPTRLMKDIGYGIDYKYAHSFPGNFVEQEFLP
                     EKLSGTSIYKPQDNSREQEIAARIKALWKGKYNK"
     gene            7174..>8135
                     /locus_tag="PLW22_09995"
     CDS             7174..>8135
                     /locus_tag="PLW22_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75074.1"
                     /translation="MKLLRKSIVLLLSLIAGTLYAQEDVAKFLKGSLNDAEKLSKAYL
                     EPFGKSLGTSLNSGWYNSANPHGILGFDITFTVPITIPPSSDKTFDVSKLNLEYWQVK
                     SGSSSTSPTVTGKSSNSILTDKATGLSELNMPDGANLKFVPAPMIQVAKGLPFHTEIV
                     GRFLPTINISSVGNFGLWGVGVKNEFKEFIPFVKRLPFSMSVLFGYTQFASSFDINKA
                     QKQKLNFKSSGYTARLLVSKSIPVLTVYGGLGYNHSTTDISLKGTYNVEGVGTVTDPV
                     AFDFANNGFTANLGLRLKLAILAFHFDYSLGNYGIFNAGVGINFR"
BASE COUNT         2354 a         1645 c         1676 g         2460 t
ORIGIN      
        1 acccaggacc ccatccttaa aagggatgtg ctctacctgc tgagctactg aatctaccta
       61 ttttgttgag aactactttt gttttttgcg ttgcaaagat agactctttc tctaaaatat
      121 acaaattttt ctttcatttc aaatgtaaat attgttctag acattgatta tcagattgat
      181 gaattatgtt aaaaagatta aatgttacac tttaacagta attcaaactc atgtaagcca
      241 ttatttaata gattagctga acaatatttt aaaataccat gacaaaggca ttaaataaac
      301 tatcgggaaa agtagttgtt attactggtg catcatcggg cattggaaag gctttaacct
      361 acgagttttc aaagtataac accaagctgg ttattggtgc tcgtaacatc gatgagctta
      421 aggctattgc atctgaacta tcctcagttg gagtagaggt gctgcctgta aaaaccgatg
      481 tgaccagcga gagtgactgt aaaaacttag ttgaacaggc cataagcagg tacggacaaa
      541 tcgatatact tatcaataac gcaggcgttt ccatgcgtgc cctttttaag gatgttgacc
      601 ttaaggttat tcggcatctc atggatgtaa atttttgggg tactgtttac tgtacaaagt
      661 atgcattacc ctatttgctt gagacaaaag gttcagtagt aggtgtgagt tccattgctg
      721 gattccacgg attaccaggc cgatgtgggt attcagcatc aaaatttgca atgcatggtt
      781 tcctggaaac cttacgtatc gaaaatctca agaacggttt gcatgtaatg attgcggctc
      841 ctgggtttac agctactaac gtcagattct cggctttaac cgccgatggt acccctcagg
      901 gtatgtcgcc tcgcgatgag aaaaacatga tgactcccga ggaggttgca aaaattattg
      961 ttaatggaat tgctaaacgc cgtaggaata ttatcatggg taccgaaggt aaaatatcgg
     1021 tactggccca acgcattctg ccaactgtga tcgatcggct tctatacgga tacatggcaa
     1081 aagagcccaa ttcgcccttt aaataggtta tttgcataat ttcccgaatt tttcttaatt
     1141 agagaaaaat ttgggtatgg ttgccttgaa catactaacc gaaacgtttt taaaccacct
     1201 aactacgcta ctatgggcga tttatatcat taccgtgctt tctacggccg caatgattat
     1261 ccatgaaaag cgcgatcccg ctaaaacgtc cacttgggtt ttacttctta tcactttacc
     1321 aatacttggg ttattacttt atatcgtatt tggccaaaac cgtagaaagg aaaagctgtt
     1381 tagccgtaaa gagattcagg atattgaaca aattgagtat ttaagccata agcaggttat
     1441 ccgcttttcc tctaaaaccg atattcacca taagcttgct gatcatttaa gtattatcac
     1501 ccttttgctc aacaacagca aggccctgct aactgaaaag aatgaggttg agattttcca
     1561 aacaggtaat caagccttcg attccataat tgaatcacta tactcagcca cttcaagtat
     1621 tcatatcgag ttttatatta ttaatgacga taagctgggt aaccgaatta aaaatatcct
     1681 gataacaaag gctaaagagg gcgttaaggt tagagtccta ttcgatgatg tagggagttg
     1741 gagcctacca aaaaggtatg tcaaggagct aaaagatgct ggcatagaga tttatccgtt
     1801 catggaggtt cgattcccct tgcttaccag tagggtaaac taccgaaacc accgaaaagt
     1861 agtagtggtc gatggaaaag ttggttattt aggtggaatg aatattgctg accggtacat
     1921 cgagggtacc cgaaggcttg gccagtggta cgacaccatg ttgaaaatcg agggtgaggc
     1981 agttcattca cttcaggtta tctttctgat tgattggtac tttgttactg gaaaaatcat
     2041 cagggaacgt aatatttact ttcctcagcc aaccgttacc agttaccatc ccttacaaat
     2101 tgtaacaagc ggtcccgatt ccgactgggc aagtattatg caagcctttt ttcatgccat
     2161 aacacgtgcc aataagcata tctatatatc tacgccttac tttataccta acgaaagtat
     2221 cctaacagcc cttaaaacag cagcactaag tgggattgat gttaggatta ttttacccgg
     2281 aaaatccgat tctaccgttg tttactggag ttcactctct tatgtttccg agctactgga
     2341 ggcaggtatt aaggtttacc tttaccaaaa tggctttaac cactccaagg tgatgatgat
     2401 tgatggtaag ctggccatgg tggggtcggc taacatggat atccgcagct ttgaggataa
     2461 ctttgaggtg gcggctttca tttacgataa tcaggttact gaaaggcttg aggagaattt
     2521 cttatccaac ctggtacaaa gccaggttat tacaccccaa agctggagta aacgtccgct
     2581 gaaaaatagc tacaaggaag cattggcacg attaattagt ccactctttt aaatctagca
     2641 cgcaatttga taatagtaga tcattaaacc tttttaaatc cgattactat gaaaagaaca
     2701 ctttttattt tagcactcac aagcctttcc ctgttctcta acgctcagtt ctttagaatg
     2761 gggcttaagg gcggcattag ctcatccaat gttaagttta gtaaggcaac cattaccgac
     2821 ggtgcaaacc aatacattgt ggagcagggc gatgctaagc ttggatttca cgcagggctt
     2881 tttgcccggg tgcaaattat gggtttcttt gttcagcctg agctgctttt ttcccgctcg
     2941 cagggcgagg ttatgcttaa ggatgttacc ctaaacgatg tttacaacga aatgcaaaag
     3001 ttcaacaagt tcgatatccc agttattgtt ggatggaaat ttggtcctgc tagggttgga
     3061 cttggaccgg ttgcaacaat cttaatctct gaaaacgatg gtcttaaaga taaattagcc
     3121 gatttgacca accaaacagt gaaaaataat ttcaggtcgg caacttttgg gtaccaggta
     3181 ggagtagggc tcgatattct taaaaccctt accctcgacc ttaaatacga aggcaacctc
     3241 agcaaacttg gaactggcat cactttgggt ggtaccgact actcattcga tcaacgcaat
     3301 ccacaaatca tcatgagcgt tggtttcttc ttttaggctt tatttaatgt cagaaggttt
     3361 ctttagaatg attctaagga aaccttttgt catttaaaac gtgtcgttta tttaaactat
     3421 aaatctattc aaggaattct taggcttttt aggcaaataa ttattgttat tgtatttaaa
     3481 aagttgaata tttgaattcg catagaataa ttatgttgga atttatgggt atcggaatta
     3541 taatcatcgt ggcgttagta tctacagcca ctacatattt cattacacat aattaccata
     3601 aaagaaaatt atctgcaaat gcagttaata gttcatccca ctcggttttg cttgaacagc
     3661 ttagggagga gattcaaaag ttaaagattc aatgtaaaga gctacaaatt gaaaacgatg
     3721 aacaggccga tacacttgca aaagcacatc tagagataca acgactcgaa gcagcagtcc
     3781 aaaatgccaa gattagggcc gaggaagccg atatcctcaa atcaaacttc ctggccaata
     3841 tgagccatga gattcgcacc ccaatgaatg gcatattagg atttgcccag ctcctaaaga
     3901 atgaggaatc gcaggaaaag cgcgataggt atatcgatat catttaccat aacggcatga
     3961 tgctggttaa tctcatagat gatattatcg atttatccaa gatagatgct ggacagctaa
     4021 ccgttaatcc tgccgactgt aacctcgata acctgatgtt tgaggtatac acatttttca
     4081 acgagattaa gttcaagcag gaaaaagagc atattaatat tcgcttactc aacctgaacg
     4141 acgatgaatc aaatatccta tacaccgatg cccaacgttt acgtcaggtt ctggttaacc
     4201 ttattggtaa tgctctgaaa tttactgaaa ggggatcggt tgagtttggt tacctgatta
     4261 acgagaataa gagcaatatt gaattttttg taagggatac aggaattggc attccaaagg
     4321 ataagcagga tattattttc gacaggttcc gtcaggtaca agaggggtca acccgtaaat
     4381 acggcggtac tggcatcggg ttgtacattt ctaaacatat cattgagttg ctcggtggcg
     4441 atatctggtt tgagtcggag gtcgggcagg ggactacatt ccacttttcc ctcccttacc
     4501 acgaatcaaa gagcgttaac gatggttctg taattttcaa agccaaaaat cctgaatata
     4561 agtgggaggg cataactatc ctgatagctg aggatattga taccaactat aaataccttt
     4621 ccactgttct agccgataca aaagccaaaa ttctttgggc aagaaatggc gaggaagcag
     4681 tatccatggt actcaatggt tccccagttg atattgttct aatggacatt caaatgcctg
     4741 tggttgatgg gtatgaggca actttaagaa taagagaaaa ttatcctgat ttaccaatta
     4801 ttgcacaaac cgcctacgct ctacctcacg ataatcttaa atgttttgag gtgggctgta
     4861 acgactatgt ttcaaagcct attaacgccc acctcctaaa gaaaaagatt aacgatttgc
     4921 taaacgttca cgttaaaagt tcttaaccac ttgcctgatc gcaacaagtc gctcaagtaa
     4981 tccttccagg tactttatat ttagcatgtt tgccccatcc gataatgctt cggctgggtt
     5041 aaaatgggtt tccataaaga gtccatcggc tcctgttgca atggcagcac gggcaatggt
     5101 ttcaatcata tcgggtctgc cacccgtaac cccggtggtt tggtttggtt gctgaagcga
     5161 gtgggtaaca tcaacaacaa caggaaatcc aaatgctcgc atggttggta tggccctaaa
     5221 atcaactatt agatcatgat acccaaacat tgttcctctg tcagttagaa taacgttggt
     5281 attgcctgaa tcaacaactt tttgcgctgc aaatttcatg gagtcgggtg atacaaattg
     5341 cccttttttg atatttacaa actttcctgt ttgggctgct gcaatcagta ggtctgtttg
     5401 tctgcacagg aatgccggaa tctgaagaat atcaacatat tcagccgcca tggcggcttc
     5461 tggggcacta tgaatgtctg taacaatggg taagccaaat cgctcattta cttcgcctag
     5521 aattttcagt gccttttcat cgccaatacc agcaaatgaa tcgattcttg aacggtttgc
     5581 cttgcggtat gaggctttaa aaatgtaagg gattttaagt ttgtctgtga tgctcgatac
     5641 agtttctgca atcctcatta cgttatcgcg gctttcaact acacaaggtc cggccatcaa
     5701 aaaaaagttg ccggattcaa tgtgcttaat tcctttaatc tttgcaatag tgttcatatt
     5761 attagcatta cttgttatac ttacctttcc atagtgcctt tatccttgca gcaatttcct
     5821 gctcacggct attgtcctgc ggtttataaa ttgaggttcc ggaaagcttt tcgggtagga
     5881 attcctgctc cacaaaattt cctggaaagc tatgggcata cttatagtca ataccatagc
     5941 caatatcctt catcaacctg gttggtgcat tgcggagatg gagtggcacg ggcaagtcac
     6001 cggtttcttt tactatccct tgtgctttgc caattgcctc gtaggctgaa ttgcttttgg
     6061 ggcttgttgc cagataaatg gttgcctcgg ataggataat tcgtgactcc ggcataccaa
     6121 tcatctgaac tgcctgaaaa cagctggtgg ccatgagtaa cgcatttggg ttagccaagc
     6181 ctatgtcctc agcggctaat ataactaacc tgcgtgcaat gaattcgggg tcctcgccac
     6241 cttcaatcat acgcgccaac cagtaaacag cagcattggg atcgctaccc cttaccgatt
     6301 taataaatgc cgatatgatg tcatagtgct gctctccgtt cttatcgtaa attgccaggt
     6361 tttgctgcag cacatcaact acggctttat tggttattac aacctggtta ttggtatttt
     6421 gtgttgatgt tataagttca atgatattat atagcttgcg ggcatcgcct cctgagaatc
     6481 tgtaaagagc atccctttca cggagttcaa acctaaattt tttcagctca ggatcggtgg
     6541 ttgttgctct aacaaaaagt agttcaagtt cctcattgga gagcggttta agtatataaa
     6601 cctggcaacg tgaaagtaat ggtgtaatca cctcaaatga ggggttctcg gttgtagcgc
     6661 caattaatgt gaaaacacct tgctccaccg caccaagcag tgagtcctgt tgcgacttac
     6721 tgaatcggtg tatctcatca ataaaaagaa tgggcgaggg tgagttgaag aatttttgtg
     6781 attttgcctt ttcaatcaca tccctaacat cttttacacc tgaacttatt gcgcttaggg
     6841 tgtaaaaggg tctatttaac tcgtttgcta caaccctagc cagagttgtt tttcctacgc
     6901 ccggagggcc ccatagaata aacgaggtta tagtacctct agttaccatt tcccgaataa
     6961 cccctttgct acccacaaga tgctcctgcc ctacataatc gttcagatta cggggtcgca
     7021 tcctttcagc taagggtatt gtcggcatgg gaaaaatatt tccacaaaat taggtaataa
     7081 tgcctttttt acggttcatt ttttaataaa tattgacatt gaactctttt tatgctttat
     7141 ttgtttaaaa ttaaaccctt taaaaaaata gatatgaagc tgttaaggaa atctatcgtc
     7201 ctgcttctaa gcctaatagc aggaacgctt tacgcgcagg aagatgttgc taaattcctg
     7261 aaaggaagcc tgaacgatgc cgaaaagctt agtaaagcat accttgaacc ttttggtaag
     7321 agtttgggaa caagcttgaa ttctgggtgg tacaattctg caaatcctca tggaatttta
     7381 gggttcgaca tcacctttac tgttccaatt acaataccac caagttccga taaaacgttt
     7441 gacgtttcaa agctaaacct ggaatactgg caggtaaaat ctggttctag ttccacctca
     7501 ccaacagtaa cgggtaaaag ttcaaactcc attttaactg ataaagcaac agggctaagc
     7561 gagttaaaca tgcccgatgg ggccaacctt aaatttgttc ctgccccaat gattcaggta
     7621 gcaaaaggat taccctttca caccgagata gttggtcgtt tcttaccaac cataaatatt
     7681 tcaagcgttg gaaactttgg tttgtggggt gttggtgtaa aaaacgagtt caaggaattt
     7741 attccttttg taaaacgatt gccctttagc atgtcggttc ttttcggtta cactcagttc
     7801 gcttcatcgt tcgacatcaa taaggcacaa aagcaaaagc ttaattttaa atcgtcgggt
     7861 tatacggctc gcttacttgt ttctaagtcc attccagttt taactgttta tggcggtttg
     7921 gggtataacc attcaacaac cgatatttca cttaagggaa cctataacgt tgaaggcgtt
     7981 gggactgtta ctgaccccgt tgctttcgac tttgccaata acggttttac tgccaatctt
     8041 gggttaaggc ttaagctagc aatccttgct ttccatttcg actactcact tggcaattat
     8101 ggtattttta atgccggggt tggaattaat ttccg
//
LOCUS       DAOOWJ010000115         8010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_8989_length_8010_cov_8.554745, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000115 DAOOWJ010000000
VERSION     DAOOWJ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8989_length_8010_cov_8.554745"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3670
                     /locus_tag="PLW22_10000"
     CDS             <1..3670
                     /locus_tag="PLW22_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU75075.1"
                     /translation="TNINSAIATIRVSATKLNFTSTPTRVGVNEPFTVKVKPTDANGN
                     ADVNFAGNVELLLGSGSGELELISENPAPIINGETQLEANYSTPGIFTLIARNPSLND
                     AVSGNITSGDADGFAAPLIQPTDTVIFTQNNSYAAAAQEIIRFKLKDPGSTDGLPLNV
                     SRIKLLAFNPSSLPELTRMVQGFVLDNGTITIAPKSYTFSGNVLQIDFNEGDVVVNDT
                     DSANFFLKVFLKDRNLVDAFRFQFYIPSSNHGWTAFDNSTAFNSTFPSTIYGRPCRIE
                     VQADRLKFLTQPFGVNPNEPFSVSVMACDSRGNVDREFQAYAALEVYNGTGSLIVNPL
                     LQPLNNGIATWNGVKLTATGTYRLKSYFGWLADAISENIYCGYNHTCPVSEYFESILP
                     AWSGIENWTTSRTDPITGEYSLVHAGDPAANQSVLSIPIETTLLGKASEWSITIRNGS
                     WDPSSENYFYLAFASTHENPTDTQAEGYAVGINPSSGNDNIAVWQFKEGKRTNIIQSA
                     FDWNENDQVKITVTLSPNGTIKLWFTPKSTGLKIFGGEAKIAVPQCSYMAFVFAYTAS
                     RAGQLWIDDMSLCTTDFAPVISSARASNLNTVRVNFSKPVSIQSAQNVQNYKLKTLTN
                     QNIGIQSIRIDGNQMVSITTQKLPFEKLKLEVRNIEDMNGYSLPDSIELGFTAQGNMG
                     RLIFNEIMANPVPSNGLPEYEYIELFNPGTDTVFTEGWKLTLNEKTVTMPADTIPPKS
                     YALVGGTTAMSNFNVYGKTIAITSFPALLNDGMLLRLYDQQENLIGFANYTKEWYNDS
                     IRNVGGYSLECIDYSNLAEGKNNWRASIASEGGTPCALNSVMAINPDVTPPQLYYIEV
                     PDAKTIRLCFSESMDSLTATLHENYTTGLGINNINIGELYTRVTVELTEPLEAEKIYE
                     ISISGLTDFAGNAIPDTAIRIGLPQLASEGDIVINEILFNPYVGGTDFVELFNRSSKC
                     FDLSQIKIANRNETDLSLKDPDSASPLPWLFFPGDYAVITTNPNQVTQFYSCQNPSSF
                     IKTSRLAAFNNDKGYVVLLNSENEVIDELRYTEKMHNKLLNDFKGVSLERINPSMPTN
                     QSSTWQSAAQAAGFATPTYRNSQYSELSGEKSNFSLTPETFSPDGDGRDDYLLISYQL
                     PEPGYVANIRVFNANGIEIYRLANNMTLGTSGQLRWDGTDTKNRRVNSGIYIVYFEYF
                     NLSGEVKREKKTCVVGYR"
     gene            3729..4517
                     /locus_tag="PLW22_10005"
     CDS             3729..4517
                     /locus_tag="PLW22_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU75076.1"
                     /translation="MEGYIELPKCTLRYRISGSGPAVVLLHGYLESLTIWEELTMELE
                     PDYTVLAVDLPGHGKSICKEEVIGMDFMADSVSAVMRAVSISKAHLVGHSMGGYASLA
                     FAERYPNWLTGLCLLHSTPNADTHEKRSARMADIKLVKEGKKEQIVENNIPRLFANHN
                     QQKLMGEINHIKSIARKTSDSGIVGALNGMAQRPDRNHIIDQALFPVTMIFGKHDNLI
                     SMEVAQTLEERHRKARTIYLENSGHMGFIEEPEQTFSAIKSILK"
     gene            4734..5192
                     /locus_tag="PLW22_10010"
     CDS             4734..5192
                     /locus_tag="PLW22_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOU75077.1"
                     /translation="MEKSKYPYGTKQPQFDRRYLQMAKIWAKNSYCKRRQVGALIVKE
                     RMIISDGYNGTPSGFENICEDETGKTKPYVLHAEANAITKVSKSSNSSEGATLYVTSS
                     PCMECSKLIIQAGIRRVVYCDEYHNLDGIELLKRAGIEVVKIEIEDINEF"
     gene            5208..6839
                     /locus_tag="PLW22_10015"
     CDS             5208..6839
                     /locus_tag="PLW22_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU75078.1"
                     /translation="MSYPNSRKDIIYPVVIAIVLVIGVALGLILGGRVEKPNLLVYPR
                     TDKLSNVIRYIADEYVDTISTETLVENTIPAVLKNLDPHSVYIPAKDLQSVNEPLEGG
                     FDGIGIQFNMNNDTVVVINTIPGGPSEKLGILAGDRIVKVNGKNIAGVKFPMDSVPKL
                     LKGPTGTRVKVSIKRTDVPDLIDFDIVRDKIPIYSVDVAYMPTKDIGYIKINTFAKTT
                     YKEFLEAMVKLTEKGMTKLILDLRSNSGGYMDAAINIANEFLPANRLIVYTQGKNRPR
                     QEAKSNGKGFFQDLPLTILIDEFSASASEILAGAIQDNDRGIIVGRRSFGKGLVQEQV
                     YFSDGSALRLTIARYYTPSGRSIQKPYKPGDEEYFYEIGNRYLHGEFQNADSIQFADS
                     LKYYTIGGRVVYGGGGIMPDFFVPLDTVGITPYYQQVSRRNLIYRYAFKFADEHRKQT
                     RALKSFDEVDRYLKKFNILTDFVGFAKQNGVEPDPNDLAQSKLIIETQLKATIARNII
                     DNEGFYPYIRYIDETLIKAIDLCEKGNCVLPLNRN"
     gene            7239..7793
                     /locus_tag="PLW22_10020"
     CDS             7239..7793
                     /locus_tag="PLW22_10020"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HOU75079.1"
                     /translation="MEIDTNQTEEIIEVGKANRLALLMLFPIAAITIIPFVIIYGFGP
                     LANGVIFLKNHQLQILVAFVLLIFVHEGLHGVTWAFFAKNGFKSIHFGIKWSYLTPYC
                     HCNEPMKRKHYMLGGIMPGLITGVIPIIIAMVTANGWLLISGIFLTAAAGGDILVLIR
                     VLKYPSNLIFMDHPKEIGFIVFKS"
BASE COUNT         2434 a         1773 c         1697 g         2106 t
ORIGIN      
        1 aaccaatatt aactcggcaa tagccacaat tagggtaagt gctacaaaac taaactttac
       61 ctcaactccc acccgtgtcg gggtgaacga accatttact gttaaggtta aacccaccga
      121 tgcaaacggg aatgcagatg ttaacttcgc aggtaacgtg gaattgcttc ttggctcggg
      181 cagtggtgaa cttgagttga tatcggaaaa tccagcgcca atcataaatg gcgaaactca
      241 gcttgaagcc aactattcca ctcccgggat attcaccctc atagcccgaa acccaagcct
      301 taacgatgct gtatcgggta acatcaccag tggcgatgcc gatggttttg ctgcaccttt
      361 aattcagcct accgatacag ttatttttac ccaaaataac agctacgctg cagcagcaca
      421 ggaaattatt cgctttaaac tgaaagaccc gggtagcacc gatggtctgc cgctaaatgt
      481 tagccgcatt aagttactcg cattcaatcc aagcagttta ccagaactaa cccgtatggt
      541 tcagggattt gttttggata atggcacgat aacaattgcg ccaaaatcgt acacgtttag
      601 cggtaatgtt cttcaaatcg atttcaacga gggtgacgtt gtagtaaacg ataccgattc
      661 ggccaacttt ttcctcaaag tctttttaaa ggacagaaac ctggtggatg cttttcggtt
      721 tcagttctat attccatcca gcaatcacgg ctggactgcc ttcgacaata gcacagcctt
      781 taacagcacc ttcccctcca ccatttatgg cagaccatgc cgcattgagg tacaggccga
      841 caggctaaag ttcttaaccc agccctttgg ggttaatccc aatgaaccct ttagcgttag
      901 cgtaatggcc tgcgactccc gcggtaacgt tgaccgggaa tttcaggctt atgcggccct
      961 tgaagtttat aatggtacgg gcagccttat tgttaaccca ctcctacaac ctctcaacaa
     1021 tgggattgcc acatggaacg gggtaaaact cacagccacc ggaacatacc ggcttaaatc
     1081 gtactttggc tggttggccg atgccatatc ggaaaatatc tactgcgggt acaaccatac
     1141 ctgcccagtt agtgagtact ttgagagtat tctgcctgca tggagcggca ttgaaaactg
     1201 gacaacctcg cgaaccgatc caataaccgg ggaatactca cttgtccatg ccggcgaccc
     1261 tgcggcaaat caatcggtac tgagtatccc tattgaaacg accctgctgg ggaaagcctc
     1321 tgagtggagt ataaccatca gaaatgggag ctgggatcca tcgtcggaaa actactttta
     1381 cctggcgttt gcctcaacac acgaaaatcc gacggacaca caggctgagg gttatgccgt
     1441 aggcataaac ccatcaagtg ggaacgataa cattgcggtt tggcagttta aggaaggtaa
     1501 gcgaaccaac attattcagt cagcttttga ctggaatgag aatgaccaag ttaagattac
     1561 tgttaccctg tcgcccaacg gaaccataaa actatggttt acaccaaaat caacagggct
     1621 taaaatcttt ggaggcgaag ctaagattgc agtacctcag tgcagctaca tggcatttgt
     1681 atttgcctac acagcgagcc gcgctggtca actttggatt gatgacatga gcctttgtac
     1741 taccgatttt gcaccagtta tcagcagcgc acgggcttca aacctaaaca cagtaagagt
     1801 aaacttttcc aaacctgttt caattcaatc ggctcagaac gttcagaact acaagctaaa
     1861 aacgctaaca aaccaaaata tcggcataca atccataagg attgacggta accaaatggt
     1921 ttcaataacc acacaaaaat tgccttttga aaagttaaaa ctggaggtaa ggaatatcga
     1981 agatatgaat ggatacagcc tccccgatag cattgaactt ggtttcacag cacagggtaa
     2041 catgggaagg ttgattttta acgagataat ggcaaaccct gtaccctcaa atggccttcc
     2101 ggagtacgaa tacattgaac tttttaaccc tggaaccgat actgttttta ccgagggctg
     2161 gaaacttact ctaaacgaaa aaactgttac aatgcccgcc gatacaattc caccaaaatc
     2221 gtatgcgctg gttggcggaa cgaccgccat gagtaacttt aatgtgtacg gtaaaaccat
     2281 cgccattaca tcgtttcctg cattgctcaa cgatggtatg ctactaaggc tgtacgatca
     2341 gcaagagaat ctgataggat ttgctaacta cacaaaggaa tggtacaacg actcaatccg
     2401 gaacgtcggt ggttactcgc ttgagtgtat cgactattca aaccttgccg agggtaagaa
     2461 caactggcgt gcaagtatag caagcgaggg aggaactccc tgtgcactaa actcggtaat
     2521 ggcaataaat cccgatgtca ccccgcctca actatactat attgaagtcc ccgatgctaa
     2581 aaccattcgt ttatgcttca gtgagtcaat ggatagccta actgcaacgc ttcacgaaaa
     2641 ctacacaaca gggctaggta taaataacat aaacatcgga gaactttata cgcgggttac
     2701 agttgaacta acagaaccgt tagaggccga aaaaatttac gagatatcca tttcaggttt
     2761 aaccgatttt gcagggaatg ctattcccga cacagcaata cggataggac tacctcaact
     2821 tgccagtgag ggcgatattg tgataaacga aatactattc aacccctatg ttggcggaac
     2881 cgattttgtg gagctattca accggtcaag taaatgtttt gacctatcgc agataaaaat
     2941 tgccaaccga aacgaaaccg acctttccct aaaagacccc gatagtgcat cacctctacc
     3001 ctggcttttt ttcccgggcg attatgccgt aattaccaca aatcctaacc aagtgaccca
     3061 gttctactcc tgccaaaacc cttcgtcttt tatcaaaact tccagattag ctgcctttaa
     3121 caacgataaa ggttacgtag tgttattaaa ttcagaaaat gaagtgattg atgaattgcg
     3181 atataccgag aaaatgcaca acaagttgct taacgatttt aagggagtgt cgcttgaacg
     3241 cataaatccc agcatgccaa ccaaccaatc aagcacatgg caatcggctg cacaggcagc
     3301 cggatttgcc acaccaacat accgtaactc acagtactct gagctaagcg gagaaaaaag
     3361 caacttcagc ttaacacctg aaaccttctc gcccgatggc gatggccgcg atgactacct
     3421 cctcattagc taccagctac ctgaacccgg atatgttgca aacatcaggg ttttcaatgc
     3481 aaacggcatt gaaatctaca ggcttgcaaa taatatgacc cttggaacct cggggcaact
     3541 ccgatgggat ggaaccgata ccaaaaaccg acgggtaaat tcagggattt acattgttta
     3601 ctttgaatac ttcaatcttt cgggcgaggt taaacgcgaa aagaaaacct gcgtggttgg
     3661 atataggtag aattttttgg ttttacaaaa taattggtgc tattttggaa aacaaaaaat
     3721 gattggttat ggaaggctat attgaactcc ccaagtgtac cctaaggtac cggattagcg
     3781 gaagtggtcc ggcagtggtg cttttacatg gttatctgga gagcctcacc atttgggagg
     3841 agttaaccat ggaactcgaa cccgattaca ctgtccttgc agtagaccta ccgggtcatg
     3901 gcaaatccat ttgcaaggag gaggtgattg gcatggattt catggccgat agcgttagtg
     3961 ctgtaatgcg ggcggtgtca attagcaaag cacatctagt tggacactcc atgggcggat
     4021 acgcaagctt agcctttgcc gaacgatacc caaactggct cacagggctt tgcctgctcc
     4081 attcaacccc caatgccgat actcatgaga aacgttcagc tcgcatggcc gatatcaaac
     4141 ttgtaaaaga aggtaaaaag gagcaaattg ttgaaaacaa cattcctcgc ctatttgcca
     4201 accataacca acagaaactt atgggcgaaa taaaccatat aaaatcaatt gcccggaaaa
     4261 ctagcgattc agggattgtt ggagcactta acggtatggc tcagcgtccc gaccgaaatc
     4321 acattattga tcaagcgcta ttccccgtta caatgatatt tggcaagcac gataacctga
     4381 tatcaatgga agtggctcaa acccttgaag aacgccatag aaaagcaaga acaatttacc
     4441 ttgaaaattc gggacacatg ggctttattg aagaaccaga acaaactttc tcagcaatta
     4501 aatcgatact aaagtaagat ttaaagaaac ttaattgttg gattaaagga aaccgctcca
     4561 cgtattagaa ttattaactt tactgctaat gcacttagga taatacataa atgctaagca
     4621 ctaactggag tcaaacaaac attttgctta tttacctgca cttgtaactg ttatttttta
     4681 ataaatttcg acattgttta aaatcgcagt ttccttaaag ttatctttat agcatggaaa
     4741 aaagtaagta tccatacgga accaagcagc cccaatttga taggcgttac ctgcaaatgg
     4801 caaaaatttg ggctaaaaac tcatactgca aacgcaggca ggttggtgcg ctaattgtta
     4861 aggagcggat gattatttcc gatggttaca acggaacgcc atcggggttt gagaatattt
     4921 gtgaggatga gaccggtaaa accaagcctt acgttttaca cgccgaggca aatgcaataa
     4981 ccaaagtgtc aaagtcgagc aatagctctg agggagccac cctttatgtt acatcatcac
     5041 cctgtatgga atgctccaag ctgattattc aggcaggcat acgcagagtg gtttactgcg
     5101 atgagtatca caatcttgat ggaattgaac tcttgaaacg tgcaggcatt gaggtagtga
     5161 aaattgaaat tgaagatatt aatgaatttt aaacaagctg aaataccatg agttacccaa
     5221 actccaggaa agatattatt tacccagtag tcattgccat tgtgctagtt attggagttg
     5281 cccttgggct gattctaggc ggaagagttg agaaacccaa cctactcgtt tacccacgca
     5341 ccgataagct cagcaatgtt attcgttaca ttgccgatga gtatgtcgat actatttcaa
     5401 cggagacttt agttgagaat accataccag cagtgcttaa aaacctcgac cctcactcgg
     5461 tttacatacc ggccaaggat ttacaatcgg taaatgaacc actggaggga ggcttcgacg
     5521 gtattggtat ccagtttaat atgaacaacg atactgttgt ggttattaac accattcccg
     5581 gtggcccatc ggaaaaatta ggaatactgg ccggcgatag aattgttaag gtaaacggga
     5641 agaacattgc cggtgtaaaa ttccccatgg atagtgttcc taagctgctt aaaggaccta
     5701 ccggaacaag agtaaaggta agcattaagc gaaccgacgt tcctgatctt atagattttg
     5761 atattgttag ggataaaata cccatttaca gcgttgatgt agcctatatg cccacaaagg
     5821 atattggtta tatcaaaatc aacacctttg ccaaaaccac ctacaaggag ttccttgagg
     5881 caatggtaaa gctaaccgaa aagggcatga ccaagcttat tctcgaccta cgcagtaata
     5941 gtggaggcta catggatgca gctattaaca ttgccaatga gtttcttcct gccaacaggt
     6001 tgattgttta cacccagggg aaaaaccgtc cgcgacagga agcaaagtcg aacggaaaag
     6061 gattctttca ggatttaccc ctcaccattc ttatcgatga attctcggca tcggcaagtg
     6121 aaattcttgc cggtgcaata caggataacg accgtggcat tattgtgggg cgtcgttcgt
     6181 tcggcaaagg attggttcag gagcaggttt acttttccga tggctctgcc cttagactaa
     6241 ctatagcacg ctattacacc ccttcggggc ggagcataca aaaaccctat aaacctggcg
     6301 atgaggaata cttttacgaa attggcaacc gttacctgca cggtgagttt cagaatgccg
     6361 atagtattca gtttgccgat tcgctcaagt actacaccat aggtggtaga gtggtttacg
     6421 gcggaggcgg aataatgccc gatttctttg ttccgctcga tacggttggt atcacccctt
     6481 actaccaaca ggtttcgcgc cgaaacctta tttaccgcta cgcctttaag tttgccgatg
     6541 agcacaggaa gcaaacccgt gcactgaaga gtttcgacga ggttgaccgt tacctcaaaa
     6601 agtttaacat cctgaccgat tttgttggct ttgccaaaca aaatggagtt gagcctgacc
     6661 ctaatgattt agcccaatcc aagctgatta ttgaaaccca gctgaaggca accattgccc
     6721 gtaatatcat cgacaatgaa ggtttttacc cctacattcg atatattgac gaaaccctga
     6781 ttaaagccat agacctttgc gagaaaggca actgtgtttt gcccttaaat aggaattagt
     6841 tgatggttgt tcgtaagaat attatcatga taagcgctag cgaaacaata ctctgtcatg
     6901 ctgagcttgc cgaagcatct ttttgctcat ttttcacttt tagctttgac tgagatcctt
     6961 cgctaacgct caggatgaca agtggaggtt gttttttgat agtgaatcct ttggaacaca
     7021 gataacgcag attttgctga attacactga gaaacaccgt gtaactttgt gtaacactgt
     7081 gatactggta tagttgatag ttgatggttt tagcaagtaa aaaggattct taagagaata
     7141 atatttatat tccctaattc aggaattatt atatcgattt aagttagctg aataaaaaaa
     7201 aattattttg gtaccacaac taggggtata ctttttaaat ggaaatagac accaatcaaa
     7261 cagaagagat tatagaggtt ggcaaggcta acaggcttgc ccttttaatg ctcttcccaa
     7321 ttgcagcaat tacaatcata ccctttgtga ttatttatgg ttttgggcca cttgccaacg
     7381 gtgtgatttt tttgaaaaat caccagctgc aaattttggt tgcattcgtt ctgcttatat
     7441 tcgtccacga gggattacat ggtgtaacgt gggctttttt tgcaaaaaac ggattcaagt
     7501 cgatacattt tgggattaag tggtcatacc ttacccccta ctgccattgc aacgaaccaa
     7561 tgaaacgcaa acactacatg ctaggaggta taatgccagg gcttattacc ggcgtaatcc
     7621 caataataat cgccatggta acagctaacg gctggttatt aatttcaggt attttcctta
     7681 ccgcagcagc cggcggcgat atcctagttc tgatacgggt gctaaaatat ccttcaaacc
     7741 tcatatttat ggatcatcct aaggagattg ggtttatagt atttaaaagc tgaaacagat
     7801 taaacaatta cccatcgtcc cattttagtt ttggggtaat gtatttaact attgcctaca
     7861 ttcaccctcc gcaacaggaa tggttttgat ttcattacgt gagactcacc gtaaactttt
     7921 tctgctaact ttgtgcataa ttaaaaaaat cacaactatg tacggaaaaa tcaaaaacga
     7981 tttacagcaa cagcttgaga atatcaaggc
//
LOCUS       DAOOWJ010000116         7775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_9354_length_7775_cov_7.457254, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000116 DAOOWJ010000000
VERSION     DAOOWJ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9354_length_7775_cov_7.457254"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   14..496
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=14..50
                     /rpt_unit_seq="gtcttaatccttgttttagtggactttactctctgac"
     gene            complement(717..2024)
                     /locus_tag="PLW22_10025"
     CDS             complement(717..2024)
                     /locus_tag="PLW22_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75080.1"
                     /translation="MATIFDYAQQLHNLCVADRYSDALKLYETKIVKQFRQREIDRSF
                     LVINCVTNAYCKTNQWNKGISYGLSKLDCLARMRTDVDYALKTIGWQLLKALIPNPPM
                     VKPETLDFEKVEQLLKMLKERAACSDIALKLYLVWIRMLPGLQVDYLTGQRFYNLFQP
                     EELINWPAEVPEKQLFKPIEVWYLNHSKYLYQHQRYQECCNLCDIGLNQANALSKDCQ
                     AWLTRRYALALKQMGDMNRAITLMEVLARKKLDWFIYHELGELYFQSNDAQNGEKCLV
                     LAYRFGGHNPSKVNLYERLGDHCMNIRMPNIATEFYQLALKVRTEEGWKIPDSLMQKL
                     PTKTTHTDSAQLFRSLLRVFQKTIEVNDMLFGAGEITRILHEGPNGDGFITDTEGNTV
                     YFRFSQANFDSDEVENGYPVKFKARRVLHRGEKRWQATKVYRD"
     gene            complement(2049..2528)
                     /gene="cas2"
                     /locus_tag="PLW22_10030"
     CDS             complement(2049..2528)
                     /gene="cas2"
                     /locus_tag="PLW22_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOU75081.1"
                     /translation="MPRKKRIPLTLTEKLKKIKTAGLSATLPPNQYDPYQEKIDDLNK
                     RIERVLGIFNKTVKTAEMVYFIMYDIENDKVRNLIAKYLKKNGCVRVQRSVFLTSSDR
                     EKFSEIHQTLKQVQEFYDNTDSIIIVPVTPDSIRTMKLIGQNINIDLALGNTNTLFF"
     gene            complement(2531..3556)
                     /locus_tag="PLW22_10035"
     CDS             complement(2531..3556)
                     /locus_tag="PLW22_10035"
                     /inference="COORDINATES: protein motif:HMM:NF040562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated primase-polymerase type B"
                     /protein_id="HOU75082.1"
                     /translation="MEPLGVELTRGNSIVSPNDPLRKIDVTDLYQLLVNPPSELESAI
                     NQLRTILSINPKKYQELKRMLPYVTCGMFNPPYRRTANFTSIGCMIVDLDHLSQHGVN
                     AEELKARLKVDPRVLLCFCSPSADGLKLLFKLSQKIYDAARYSMAYKMFITQFANQHG
                     VAHLVDRVTSDVTRATFISIDANAYFNPNAEAVPLHTLINFDSPSSINTAQKQIQEFE
                     FSLPPENTAPKEKNILPSDILQKIKESLNPNIKTKAEKIIYVPQELEPAVEKVKSRMQ
                     ELGISVKQIENIHYGKKFVFELDGRFAQLNLFYGKQGYKVVVTPVRNADAELAEVSRL
                     ILCEIFM"
     gene            complement(3562..4539)
                     /gene="cas1"
                     /locus_tag="PLW22_10040"
     CDS             complement(3562..4539)
                     /gene="cas1"
                     /locus_tag="PLW22_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOU75083.1"
                     /translation="MELVLNTFGTSLQVEDGLFLVVSKDGKQTVDPSTVKSILINRGA
                     KISSDAALLAIANEIDVLFVDATGKPGGRLWSIKYGSISEIRRAQLEFLYSPKAVKWI
                     KNLLRTKIDNQIGLLLSLDAEHENQKVALEQCVNRLRDYQTKIEGVDAPFVNDIAPSL
                     RGWEGAASRAYFATLNLFLPPEYQFKERSQHPATDVFNCLLNYAYGILYGKVEGALIK
                     AGIDPYAGIFHRDDYNRPALVFDVIELYRIWADYVVFSLCCQKVIDQECFSIKDDGSY
                     WLENMGKRILIQSMNDYLDEIIYIKGIARSRLNHIELQAHSLAQTFLKG"
     gene            complement(4548..5951)
                     /locus_tag="PLW22_10045"
     CDS             complement(4548..5951)
                     /locus_tag="PLW22_10045"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU75084.1"
                     /translation="MNLNKEQKDALAKLIEFVKGNEHDTFILKGYAGTGKTVLVNELL
                     QSPEIKEIPVQLLASTGRAAKILSLKTNRAAQTVHSYIYTFVYDEFNEEKKTIKVQFA
                     LRELNIDLTDTIYIVDEASMLSNHKDNTMEEGLQFGSGKLLTDFFTFCQGRKVIFVGD
                     PAQLPPINTLFSPALNEEYLQNTFKRKVTSTFLTQVMRYSKTSGIYYNTSRIREQLEK
                     PVKPKYLSIIATQFPDMDVVSLDYMLIENYVNTIKKKGYESAILVAYTNKTCNQLNEL
                     VRKKLFTNYQLPQKGDLCIVAKNNYLHGIENGQHIYIQSISEKEERVGQLHFRDIIYY
                     LITPNGIDKSREYCGKVIMEFLLREKPTITHEEEYELYKRFFATIDPEKRKNKQYVID
                     AFSKDPYIHALRIRYGYAITCHKAQGGEWDTLFLILEKVLFGQTKDNPEFLYRWVYTA
                     LTRPVNKVVLLDNICIK"
     gene            6126..6452
                     /locus_tag="PLW22_10050"
     CDS             6126..6452
                     /locus_tag="PLW22_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75085.1"
                     /translation="MNALDIMPFGRNKGYQIGMVYLFDPGYINWALAETDKFCITDME
                     SLEQFKPFVNPSTGFYYEYLALDELNFFIHYFDFTELLYLGSVDFKFSEKAKMQNLFK
                     LKRFGH"
     gene            7024..7701
                     /locus_tag="PLW22_10055"
     CDS             7024..7701
                     /locus_tag="PLW22_10055"
                     /inference="COORDINATES: protein motif:HMM:NF036560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HOU75086.1"
                     /translation="MKKLRSLYIGFENPIASSEVVAFRSAIAEKAGRKHILFHNHLDS
                     NEYLYSYPRIQYKVVNQRPAIVCIEEGVDEIHHFFQKPNRDLMLYDRLYNVKVAKLQV
                     NQFTMQVWETPFTYNLYRWHALNQENYTLFRNLTDDNARHELLERILTGNILSFAKGI
                     GWTIDKEVKVKVNAIKKQRWLQLKDKKVLGFDIEFTTNVFLPNYIGLGKNVSIGFGIV
                     KQKKTHK"
BASE COUNT         2048 a         1574 c         1570 g         2583 t
ORIGIN      
        1 aataaataat atagtcttaa tccttgtttt agtggacttt actctctgac agtcatatca
       61 gccatcctat ccaataaatg gctaacgcgt cttaatcctt gttttagtgg actttactct
      121 ctgacttatt taaaatacca tttacagatt tagaaatact tgtcttaatc cttgttttag
      181 tggactttac tctctgactg ctaccattac catttcagcc aactctaggg aacaaggtct
      241 taatccttgt tttagtggac tttactctct gacctacctg gacgaaattt cctagcagca
      301 ttctcccccc agtcttaatc cttgttttag tggactttac tctctgacgg ccattgaggt
      361 ggattttggg gggaggggcc acctgtctta atccttgttt tagtggactt tactctctga
      421 ccttttaaaa tataaagtta tattcatgta agtccttacg tcttaatcct tgttttagtg
      481 gactttactc tctgactgta ctttttgaaa actattgtaa aacagtatgt tacaatacgg
      541 ttttaacaaa actttaaact ttttagctga atttggggtt gtttttttgg gggtgcaaag
      601 gtacatactt tttggttttg ttttacaagt tggtttcatt tttatttcag catcttgcaa
      661 tcaaagttaa aaaaactttt ttccaagatt ttcatttgaa caactttttt ttaaaattaa
      721 tcgcggtaca ccttggtagc ctgccaccgt ttctcgcctc ggtgtagaac ccttcttgct
      781 ttaaatttta cggggtatcc attttcaacc tcatcggagt caaaattggc ttgggaaaac
      841 ctgaagtata ccgtattacc ctcggtatcg gttataaaac catcgccatt ggggccctca
      901 tgaagtatgc gggtaatttc tccggctcca aaaagcatgt cgttcacctc aatggttttt
      961 tggaataccc ttagtagcga tcggaaaagc tgtgccgaat cagtatgggt tgttttggtg
     1021 ggtagttttt gcatgaggct atcgggtatt ttccagccct cttcggttct taccttcagg
     1081 gcaagttgat agaattctgt cgctatattt ggcatcctta tattcatgca atggtcgccc
     1141 aaacgctcgt agaggttaac cttgctcggg ttatgcccac caaacctgta tgccaggacg
     1201 aggcattttt cgccattttg tgcatcgtta ctctggaaat atagttcgcc cagttcgtgg
     1261 tatataaacc agtccagttt ctttcgagct aacacttcca ttagggtgat agcgcgattc
     1321 atgtcgccca tttgctttaa agctaatgca taccttcggg ttagccaagc ttggcaatcc
     1381 ttgcttagag catttgcttg gtttaacccg atatcgcaca ggttgcagca ttcctggtat
     1441 cgttggtgct ggtaaaggta cttggagtgg ttcaggtacc aaacctcaat aggtttaaag
     1501 agttgctttt cgggcacttc agctggccag ttaatcagtt cttccggttg gaacaggttg
     1561 taaaatcgtt gtcctgttaa ataatcaacc tgcaatcctg gtaacatcct gatccatacc
     1621 aggtatagct ttaatgctat atcgctgcag gcggccctct ccttaagcat tttcagcaat
     1681 tgctcaacct tttcaaaatc taaagtttca ggtttaacca ttggtggatt tggaatcaat
     1741 gcctttaaca attgccaacc aattgttttt aaggcgtaat caacatcggt cctcattctt
     1801 gctaaacaat ccagcttact taacccatag cttatccctt tattccattg gtttgtcttg
     1861 cagtaggcat tggtaacgca gtttataacc aggaaacttc tatctatctc cctttgtctg
     1921 aattgcttta ctatttttgt ttcatacagt ttaagcgcat cgctatatcg atctgctaca
     1981 cacaggttgt ggagctgttg agcgtaatca aaaattgtag ccatgtttgt tttggttttt
     2041 gttattagct aaaaaaacag ggtgttggtg ttcccaagcg caaggtcgat gttaatgttc
     2101 tgtcctataa gtttcatggt tcttatgcta tcgggcgtta ccggaacaat tattatacta
     2161 tcggtattgt cgtaaaactc ctgtacctgt ttcagcgttt gatggatctc ggaaaacttt
     2221 tccctatccg atgatgttag gaaaaccgaa cgttgaaccc ttacacatcc attcttttta
     2281 aggtacttag ctattaggtt tcgaacctta tcgttttcta tgtcgtacat tataaagtaa
     2341 accatctctg ctgtttttac agttttgtta aagattccta gcactcgttc aatgcgcttg
     2401 ttcaggtcgt caattttctc ctggtatggg tcgtactggt tgggtggcag ggtagcactt
     2461 aacccggctg ttttaatttt tttaagtttt tcggttaggg tgaggggtat gcgtttcttg
     2521 cgcggcatgg ttacataaaa atttcgcaaa gtattagtct gcttacttcg gcaagttcgg
     2581 catcggcatt acgaactggt gttaccacca ctttataccc ttgcttaccg taaaacaggt
     2641 taagctgtgc aaaccggcca tcgagctcaa atacaaattt tttcccgtaa tggatattct
     2701 caatctgttt aacgcttatg cccagctcct gcattcggct ttttactttt tccacggcgg
     2761 gttcaagctc ctgtggcacg taaataattt tttcagcctt tgtttttatg tttgggttca
     2821 ggctttcctt tattttttgg aggatatcgg atggtaaaat gtttttttct tttggggcag
     2881 tattttcagg aggtaagcta aattcaaatt cttgtatttg cttttgggcg gtgttgatag
     2941 agctggggct atcaaagttt ataagtgtat gcaggggtac cgcttcggcg ttaggattaa
     3001 agtacgcatt ggcatcaatg ctaatgaagg tggcacgggt aacatcggag gtaaccctat
     3061 caaccaaatg cgcaactcca tgctggtttg caaattgtgt aataaacatt ttataggcca
     3121 tgctgtacct tgctgcgtcg taaatctttt ggctgagctt gaataatagc ttgagtccat
     3181 cggcgcttgg tgagcaaaaa cacagcagta cgcgggggtc aacctttaac cttgctttta
     3241 actcctccgc attaacccca tgctgcgata gatgatccag atcaacaatc atgcatccaa
     3301 tggaagtaaa gttagcggtt cgcctgtagg gtgggttaaa cattccgcag gtaacgtatg
     3361 gcagcatacg ctttagctcc tggtatttct tgggattgat agataatatg gtgcgcagct
     3421 ggtttattgc cgattccagc tccgatggtg ggttaaccag caactggtac aggtcagtta
     3481 catcaatttt ccgtaacggg tcgttggggc ttacaatgct gttccccctt gttagctcaa
     3541 ctcctaacgg ttccatattg cttatccttt taaaaatgtt tgtgctaagc tatgagcctg
     3601 tagttcaata tggtttaaac gacttcgggc tatacctttt atatatataa tttcatccag
     3661 gtagtcgttc attgactgaa taagaattcg tttccccata ttttcgagcc agtaggagcc
     3721 atcgtcctta atactgaagc attcctggtc aataaccttt tggcaacaga ggctaaaaac
     3781 tacgtaatcg gcccaaatgc ggtaaagttc aataacatca aaaactaatg ccgggcgatt
     3841 atagtcgtcg cggtggaata ttccagcata ggggtcaatt cctgctttaa taagagcccc
     3901 ttcaaccttt ccgtacagta tgccataggc atagttaagt aggcaattaa acacatcggt
     3961 tgccgggtgt tggcttcgtt ccttgaattg gtattcaggt ggcaaaaaca ggttaagggt
     4021 ggcaaagtaa gcacgcgatg ctgctccctc ccaacccctc aggctggggg caatgtcgtt
     4081 cacaaagggg gcgtcaactc cttcaatttt ggtttggtaa tcgcgtaacc tatttacaca
     4141 ttgttccaat gcaacctttt gattttcatg ttccgcatca aggctgagca acaaaccaat
     4201 ctggttatca atttttgttc gtagtaagtt tttaatccat tttacagcct taggagagta
     4261 aagaaattcg agttgagccc ttcggatctc cgaaatggat ccatacttaa tgctccacag
     4321 ccggcctccg ggtttaccgg tggcatctac aaatagcacg tcaatctcat tggctatggc
     4381 aagcaatgcc gcgtcgctgc ttatttttgc cccacggttt attagtatcg attttactgt
     4441 ggaggggtca acggtttgct taccatcctt actcaccaca aggaatagcc catcctcaac
     4501 ttgtaggctt gttccaaagg tgttaagtac aagttccatg gcttaatcta ttttatgcag
     4561 atattatcga gtaaaactac cttgtttacc ggtcgggtta atgctgtgta aacccaacgg
     4621 tataggaatt ctggattatc cttggtttgt ccaaataaaa ctttttctag tatcaggaat
     4681 agagtatccc attcaccccc ctgtgcctta tggcatgtaa tagcataacc atacctaatc
     4741 cttaatgcat gaatatatgg gtcttttgaa aaggcatcaa ttacgtattg tttgtttttt
     4801 cttttttctg gatcaatggt tgcaaaaaaa cgtttatata gctcatactc ctcctcatgt
     4861 gttattgtgg gtttttcgcg gagtaaaaat tccattatta ccttcccgca gtattccctg
     4921 gatttatcaa taccatttgg ggttatcagg tagtatatta tatccctaaa gtgtaattgc
     4981 ccaacacgct cttctttctc cgatatgctt tgaatgtaga tatgctgacc gttttcgata
     5041 ccatggaggt agttgttttt ggccacaatg caaaggtcgc ctttttgtgg gagctggtaa
     5101 tttgtaaaaa gctttttacg caccagctca ttcagctggt tacaggtttt attggtataa
     5161 gcaaccagta tagcgctttc gtaacctttc ttcttaattg tgtttacgta attctcaatt
     5221 agcatgtagt caagcgatac tacatccata tccgggaatt gggtggcaat aatgctcaag
     5281 tattttggtt ttacaggttt ttcaagttgc tccctaattc gtgaagtgtt gtagtaaatg
     5341 cccgatgttt ttgagtatcg cattacctgg gtaaggaacg ttgaagtaac cttacgctta
     5401 aaggtgttct gtaggtattc ctcattcagt gcaggcgaga atagggtgtt aatgggtggt
     5461 agctgcgctg gatcgcccac aaaaataact ttgcgtcctt ggcaaaaggt aaagaaatcg
     5521 gttagcagct taccgctacc aaactgcaat ccctcttcca tggtgttatc cttatggttc
     5581 gataacatcg atgcctcatc aactatataa atggtatcgg tcaaatcaat gttgagttct
     5641 cttagcgcaa attgcacttt tatagttttc ttctcctcgt taaactcgtc gtaaacaaag
     5701 gtgtatatgt agctgtgaac ggtttgagca gcacggttgg ttttgagcga gaggattttt
     5761 gccgctcttc cggttgatgc aagcagttgc acaggtatct cctttatttc aggactttgt
     5821 aatagctcat taaccaacac agtttttccg gttcctgcat acccctttag tataaaggta
     5881 tcgtgctcat tgccttttac aaattcaata agttttgcaa gggcatcctt ttgttctttg
     5941 ttcaggttca ttgcaataat attttagaat attatgtttt gggcttattc ataggatgtt
     6001 aagctataat tcataattac tcacagtttg cctagcgcat tcgttaaaat aataagttga
     6061 agatataatt tttttcaatt tattatattc gtaatagtta accttaccta cttattaaaa
     6121 aaattatgaa tgctcttgat ataatgccat tcggcagaaa caaaggctat cagataggga
     6181 tggtttatct ctttgatcca ggctatataa attgggcatt ggccgaaacc gacaaatttt
     6241 gtataactga tatggaaagc cttgaacagt ttaagccatt tgttaaccct tcaacaggct
     6301 tttactatga gtatcttgcc ctcgatgagt tgaacttctt tatccactac tttgatttca
     6361 ccgagttgct ttacttgggg tcggttgatt tcaagtttag cgagaaagca aaaatgcaaa
     6421 acctatttaa gcttaaacgt tttggccact aagcgtcaaa accttactgg gcaaatacaa
     6481 aacccgaaga tcagatttac tttctccagc tacttgatga agatcttccg agttgtgagc
     6541 ttgaggttaa gtacatgggg aagcgtttaa cccaacgtaa tttcccatac tatctgctaa
     6601 cattcgataa ttctaaggga ataattaact tcctgcccca ttgctccaat tgcaagctcg
     6661 ccctactcaa ggatgatgaa gttgatattg aaccagtttt tatcaagttg accgaaggtg
     6721 gtacatacag gtttttaaat gacaaccaag gcttttcaat agggtaaagg tataaccagg
     6781 ttagttatat gcgtccataa attgtttgat gttaccattt cttttagttc aagcatatct
     6841 ttagttctat tttaaagagg gtttaaaaaa tagtacatgc caattgcatt agaccacttg
     6901 ttaacaacct gttaataaaa aatgcttacc ataagttttg tcaattttaa atttattatt
     6961 taatttagga tagcggttgg gattgttggg tgtgggagta cttaacctca taataaccta
     7021 acattgaaaa agttacgctc gctatacatt ggttttgaaa atcccattgc cagctcagag
     7081 gttgtggcat tccgttcggc aatagccgag aaagccggca ggaaacacat actattccac
     7141 aaccatctgg acagtaatga gtatctatac agttatcccc gcattcagta caaggtagta
     7201 aaccagcgcc ccgccatagt atgcatagag gaaggggttg atgaaatcca tcactttttt
     7261 caaaaaccca acagggatct catgctatac gacaggcttt acaacgtaaa ggtggccaag
     7321 ttgcaggtca accagtttac catgcaggta tgggaaaccc ctttcaccta taacctgtac
     7381 cgctggcatg cccttaacca ggaaaactat acgcttttcc gcaacctaac cgacgataat
     7441 gcaaggcatg agctgcttga acgcattctt actggtaaca tcctttcgtt tgccaagggc
     7501 ataggttgga ccatcgataa agaagtaaag gttaaggtta atgctataaa aaaacaacgt
     7561 tggctacaat tgaaggacaa aaaggtgctt ggtttcgaca ttgagtttac aaccaacgtt
     7621 tttctcccta actacatagg gttgggcaaa aacgtaagta taggttttgg aattgtgaaa
     7681 caaaaaaaaa cgcataaatg aataacgata aagataaaat acgtcagcaa atatatttag
     7741 gtgctcttct gcatgatatt gggaagtttt atcaa
//
LOCUS       DAOOWJ010000117         7571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_9699_length_7571_cov_9.339941, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000117 DAOOWJ010000000
VERSION     DAOOWJ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9699_length_7571_cov_9.339941"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLW22_10060"
     CDS             complement(<1..448)
                     /locus_tag="PLW22_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU75087.1"
                     /translation="MPRTKQQVEAIRQQRVMQIFDAALDVFAHKGFHSATIADIAQKA
                     GIAKGLLYHYYSSKEELLSHIVINGMNNLIEKFDENHDGVLTDAEMELFINDMFRKIV
                     ENRNYWKLYFSVMLQPNVFSKELMQKLEGIYEPMVSILIQYFRRKGY"
     gene            857..1630
                     /locus_tag="PLW22_10065"
     CDS             857..1630
                     /locus_tag="PLW22_10065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU75088.1"
                     /translation="MEQENNSIHDFDIALICEYFSYVERQGPGSPEVTIKALSFIDNL
                     TQNAKIADIGCGTGGQTMVLAQNTPGTIIGIDLFPQFVDIFNQNATKLGLTDKAKAIV
                     GSMDNLPFQVEELDLIWSEGAIYNIGFERGLTEWRRFLKKGGYVAVTEASWFTPERPA
                     EIEQFWLDAYPEIDTIPSKVAIMQKAGYIPVATFILPENCWTEHFYAPQVTAQKLFME
                     KYAGNQNAMRLVENLLHEAQLYARYKDYYGYVFYIGKKM"
     gene            1931..3847
                     /locus_tag="PLW22_10070"
     CDS             1931..3847
                     /locus_tag="PLW22_10070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOU75089.1"
                     /translation="MSVGYSEDSIKTLEWQEHIRKRPGMYIGKLGDGSQPDDGIYILL
                     KEVVDNSIDEFMMGNGKRIDIQLNEKTVSIRDYGRGIPQGKLIDVASKMNTGAKYDSK
                     VFKKSVGLNGVGIKAVNALSNQFIIKSIREGLVREAEFAGGILVKDKQYPANGEANGT
                     YVQFTPDEAVFGQYSFQDEYIETMIRNYTYLNTNLTISFNGKEFISKNGLLDLLNENI
                     TTEPLYPIIHLKGEDIEVAITHGVGYGEDYYTFVNGQHTTQGGTHLTAFREAYVKTIR
                     EFYKKDFDPSDIRTSIIAAISIKVEDPVFESQTKTKLGSKDMGPNGPSVKQFITDFIK
                     EKLDNFLHRNSETAQILLQKIIESEKERKAISGIKKIARERAKKASLHNRKLRDCRIH
                     YNSKDDRAHESTLFITEGDSASGSITKSRDVNTQAVFSLRGKPLNTYGLTKKIVYENE
                     EFNLLQAALDIEEDMENLRYNKIVIATDADVDGMHIRLLLITFFLQFFPELIRQEHLF
                     ILQTPLFRVRNKKKTVYCYTPEEKEKAIAELGPNPEITRFKGLGEISPDEFKNFIGPD
                     MRLDPVRLTKEDQIHDLLEFYMGKNTPDRQDFIINNLRIEEDIIDEEPAPKADENNID
                     EEGNHDVENKNLVA"
     gene            3874..6528
                     /locus_tag="PLW22_10075"
     CDS             3874..6528
                     /locus_tag="PLW22_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOU75090.1"
                     /translation="MRLDDFEADELLSEGEGKPVSPELHAKIERITGEAAKKTHITGM
                     YKDWFLDYASYVILERAVPHLDDGLKPVQRRILHAMKRLDDGRYNKVANIIGYTMQYH
                     PHGDASIGEALVQLGQKDLLIDTQGNWGNILTGDSAAAPRYIEARLSKFALEVVFNPK
                     TTEWMLSYDGRNQEPVTLPVKFPLLLAQGVEGIAVGLASKILPHNFNELIDAAISYLK
                     GKDFELYPDFPTGGLADCSRYNDGLRGGAVKVRARIQKLDRKTLAITEIPFGKTTTSL
                     IESILKANDKGKIKIKRIDDNTAENVEILIHLHPDANPDMTIDALYAFTDCEMSISTN
                     SCVIYDDKPRFMGVKDILRASVDRTKELLTMELNIRLAELEEDWHFSSLEKIFFEERI
                     YRELEKDTETWDLVIEAIERGFDPYRKLLRREITRDDILKLTEKPVRKISKFDIKKAD
                     EHIKAVEAEMEQVKHHLANIVTYTIDYYQQIKKKYGKGRDRKTELRSFDTIVATKVVV
                     ANEKLYVNRAEGFVGTGLKKDEYVCDCSDIDDVIVILRNGKYYIRKVQEKDFFGKDIV
                     HVGVYKKNDTRTIYNVVYRDGLNGDIMMKRCAINGTTRDKEYDITKGTQGSQILWLSA
                     NPNGETEVLKVYLKPRPRLRNLILELDFSQLAVKGRGSQGNIFTRYAIHKIMLKEKIA
                     GAVEGVKVWYDPEVMRLNHEGQGELLGEFLPDERVLVVNPDGTYFLANTNYALRFDVQ
                     PLLVEKFNPHKVFSAVYFDGEQKQYYLKRFRFEPTEKTQSFIPDEYSSKLIALNADVF
                     PALKLVFGGKNANREPENIDVDAFIAVKGYKAKGKRLTTFDVKRVEFIEPLEKERSAF
                     EEETSGIPDSESEDNDSGSSAEQMSLEL"
     gene            6642..7154
                     /locus_tag="PLW22_10080"
     CDS             6642..7154
                     /locus_tag="PLW22_10080"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOU75091.1"
                     /translation="MKIFLIGFMSSGKTTVGRELAHFLSYQFLDLDEYIETKHNRTIK
                     QIFETKGEEHFRLIENEALKEVCSFNGDFVISSGGGTSCFYNNIDYMNRNGITVYLRL
                     EVSTLVSRLVESKIDRPLLWGKTREELNDYIIRVLDERKKFYEKAQIIVDADNTKPID
                     LAKLIISALE"
     gene            7276..>7571
                     /locus_tag="PLW22_10085"
     CDS             7276..>7571
                     /locus_tag="PLW22_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75092.1"
                     /translation="MRYILGGLCLVLTFNTPVFAQVENQSLNSDSIYEQRQINQMDSL
                     LYQWYIQQPNEDNSLVADIEGDTIEGPELPDSFYIKRLQTINSLIDLPYNNIVR"
BASE COUNT         2335 a         1650 c         1653 g         1933 t
ORIGIN      
        1 gatagccctt gcgcctaaag tactgaatta agattgatac cattggttca tagataccct
       61 caagtttttg catcaactcc ttgctgaaaa cgttgggttg aagcataacg gagaagtaca
      121 acttccagta gttgcggttt tcgacaatct tacggaacat atcgttaata aacagttcca
      181 tctctgcatc ggttagtacc ccatcgtggt tttcatcaaa cttttctatc aaattgttca
      241 ttccattaat cacgatatgg gagagcagct cctccttgga gctatagtaa tggtaaagca
      301 acccttttgc aattcctgcc ttttgggcga tgtcggcaat ggttgccgaa tgaaaaccct
      361 tgtgggcaaa aacatcaagt gcagcgtcaa aaatttgcat aactcgctgc tggcggattg
      421 cttcaacttg ctgctttgtg cgtggcatat ttatttttgg ttgaacggtc ggtcaaatat
      481 aaaaaattgt tttttaccat tcaatttttt tggaagaaaa tttttacgtt tgattcctga
      541 attgataaac actacgaaaa atcgagatta tcaataaaaa aatctattca atctacccta
      601 aacttaactg atacataatt tgttgtaaaa caaaattgaa actcaaaaaa atatgcttac
      661 ctttgaaagg tctaaaactt ttggatgtat ttatcgaatt cggagctggt aggttaggta
      721 atcttgagta aatcacaaga ttacacaacc atttctgttt ggcagcaact tgccatgtaa
      781 tatcttttat aaattttcaa acaatttaat ggcggcaaag ccatgctttt gcctgttaaa
      841 aaataactac tagatcatgg aacaagaaaa caactccata cacgatttcg atatagcatt
      901 aatatgtgag tatttttcgt atgtcgaacg tcaagggcca ggcagccctg aggtaacaat
      961 caaggcattg agttttatcg acaatcttac ccaaaacgcc aaaattgccg atataggatg
     1021 cggaacaggg gggcaaacca tggtgcttgc ccaaaacaca ccgggaacaa taataggtat
     1081 tgacctgttc ccacaatttg ttgatatttt caaccagaat gctaccaagc taggcctaac
     1141 ggataaagcc aaagcaatag ttggatcaat ggacaacctg ccctttcagg tcgaagagct
     1201 agaccttata tggtcagagg gagccatcta taacattggt tttgagcgcg gcctaaccga
     1261 atggcggcga ttcctcaaga aagggggcta cgtggcagta actgaagcat cgtggtttac
     1321 gccagagcgt ccagcagaaa ttgagcagtt ctggttagat gcttacccgg aaattgacac
     1381 cattccaagc aaggtggcaa tcatgcaaaa ggctggttat attccggttg ctactttcat
     1441 actgcccgaa aactgttgga ctgagcattt ttatgctcca caagtaacag cacagaaact
     1501 tttcatggaa aagtatgcag gtaatcagaa tgccatgcga ttagttgaaa acctactaca
     1561 cgaggctcag ctctacgcta ggtacaagga ttactatggc tatgtttttt acattgggaa
     1621 gaaaatgtag ttgattagaa ggaaggggct acaccctcaa atgccgtagc cctctctttt
     1681 tttacacatc cacacagata tttacaagaa acaataccca aacattgccg ttaacgcata
     1741 gctaattctc ccatgtttcg gaaacaacga gtttggttta atgatttaaa accgcaagcc
     1801 aaattattac aataactcat taaattccaa tcggtttaca agtcgcccaa acctgttgat
     1861 atttatttgg gtaaaaaaag ttcatgccaa ttcaatttgg ttatttttgc agcaaactgg
     1921 agaagtagtc atgagtgttg gatattctga agatagtatt aaaacgttgg aatggcagga
     1981 gcacattcgc aagcgcccgg gtatgtatat cggtaagctt ggcgacggct ctcagcccga
     2041 cgatggcatt tacattctgc ttaaggaggt ggttgacaac tccattgatg agttcatgat
     2101 gggaaacggt aaacgtatag acattcagct taacgagaaa actgtttcca ttcgcgacta
     2161 tggccgtggt atcccgcaag ggaaactaat tgacgtggcc tccaagatga acacgggtgc
     2221 caagtatgac tccaaggttt tcaagaaatc ggtaggctta aatggggttg gtataaaggc
     2281 ggttaatgcg ctttccaacc agtttatcat aaagagtatt cgcgagggtt tagttcgcga
     2341 ggccgagttt gccggcggca tccttgttaa agataagcaa taccccgcca atggcgaggc
     2401 aaacggtact tacgttcagt ttactcctga cgaggcggta ttcggtcaat actcattcca
     2461 ggatgagtat atcgaaacca tgatccgaaa ctacacctac ctcaacacca acctaaccat
     2521 cagctttaac ggcaaggagt ttatctcaaa aaacgggctt ctcgacctac tgaacgaaaa
     2581 cataacaaca gagccactct accccattat tcacctgaaa ggtgaagata ttgaggtggc
     2641 aattacccac ggtgttggct acggcgaaga ctactatacc tttgtgaacg gccagcatac
     2701 cactcagggc ggtacacacc taaccgcatt ccgcgaagcc tacgttaaaa ccatccggga
     2761 gttctataaa aaggactttg acccatcgga tattcgtact tccattatag cagccataag
     2821 cattaaggtt gaagatcccg tatttgagtc acaaaccaaa accaagcttg gcagcaagga
     2881 catgggccct aacggaccct cggtaaagca attcatcacc gattttatta aggaaaaact
     2941 cgataatttc ctgcatcgca atagcgaaac tgcgcaaatt ctgctacaaa agattattga
     3001 gtccgaaaag gaacgtaaag ctatatcggg tatcaaaaag atagcccgtg agcgggctaa
     3061 aaaggcaagc ctgcacaacc gcaagctacg cgattgcagg atccattaca acagcaagga
     3121 tgaccgtgcg catgaaagta ccctttttat tacagagggc gactccgcaa gcggttcaat
     3181 caccaaatcg cgcgatgtaa atacacaggc agtattctcg ctccgcggta aaccgttaaa
     3241 cacctacggg ttaaccaaaa agatagttta cgagaacgag gagtttaacc tgctacaagc
     3301 tgcgctggac attgaggagg acatggaaaa cctccgctac aacaaaattg taatagccac
     3361 cgatgccgat gtggacggta tgcacatcag gctgctgctc ataacctttt tcctacagtt
     3421 cttccctgag ctaatccgcc aggagcacct cttcatcctt caaaccccac ttttcagggt
     3481 tcgtaataag aagaagaccg tttactgcta tacccccgag gagaaggaaa aggcaatagc
     3541 cgaacttggc ccaaaccctg aaataacaag gtttaaaggg ttaggtgaaa tttcgcccga
     3601 tgagtttaag aacttcattg gccccgacat gaggctcgac ccggttcgtt tgaccaagga
     3661 agaccaaata catgaccttt tggagttcta catgggcaag aacacgcccg accgtcagga
     3721 tttcattatc aataatctca gaattgaaga ggatattatt gatgaggaac cagccccaaa
     3781 agccgatgag aataacatcg acgaggaagg caaccatgac gttgagaata aaaacctggt
     3841 agcataaaca tcaacctaca atagctcaag aaaatgcgat tggacgattt tgaagcggat
     3901 gagcttttaa gcgaaggcga ggggaaacct gtttcgccag aacttcatgc caaaatagaa
     3961 aggattaccg gcgaggccgc caaaaagacc cacatcaccg ggatgtacaa ggactggttt
     4021 ttggactatg cctcgtatgt tatactggaa cgcgccgttc cacacctcga tgacgggtta
     4081 aagcccgtgc agcgccgtat actgcacgcc atgaaacggc tcgacgatgg gcgatacaat
     4141 aaggtggcca atattatcgg ttacaccatg cagtaccatc cccacggcga cgcatcaatt
     4201 ggcgaagcct tggttcagct aggccaaaaa gatttgttga ttgacaccca gggtaactgg
     4261 ggcaacatac ttacaggcga cagtgcagcc gcaccccgtt acattgaggc taggctatca
     4321 aaattcgccc ttgaggtggt tttcaacccc aaaaccactg agtggatgct cagctacgac
     4381 ggccgtaacc aggagcctgt aacgcttccg gttaagttcc cgcttttgct agcgcaagga
     4441 gtggaaggta ttgcggttgg tttagcctca aaaattttac cccacaactt taatgagcta
     4501 attgatgcgg caatcagcta cctaaagggt aaggattttg aactttaccc cgatttcccc
     4561 acaggtggct tagccgattg tagccgttac aacgatggtt tacgcggcgg agcggttaag
     4621 gtacgcgccc gcatccaaaa actcgaccgt aaaacccttg ccataaccga gattcccttt
     4681 gggaaaacca ccacatcgct tatcgaatca atccttaaag cgaacgacaa agggaagatt
     4741 aaaatcaaaa ggattgacga taataccgct gagaatgtag aaattctcat ccacctgcac
     4801 cccgacgcca accccgatat gacaattgat gccctttacg cctttaccga ctgcgagatg
     4861 tcaatatcaa ccaactcatg cgtcatttac gacgataagc cccgtttcat gggcgttaag
     4921 gatatcctta gggcttcggt ggatagaact aaggaactcc ttaccatgga acttaacatt
     4981 aggctggcag agctggagga ggattggcat ttctcgtcgc tggaaaagat attctttgaa
     5041 gagcgcattt atcgtgagct tgaaaaggat acagaaacat gggatttggt aatagaagcc
     5101 attgagaggg gttttgaccc ttaccgcaaa ctactccggc gcgagattac ccgcgacgat
     5161 attctgaagc taaccgagaa gcccgtgcgc aagatatcga aattcgatat caaaaaggcc
     5221 gatgagcata tcaaggccgt ggaagccgaa atggagcaag ttaagcatca ccttgccaat
     5281 atagtaactt ataccatcga ttactaccag caaatcaaaa agaagtacgg gaaaggaaga
     5341 gatcgcaaaa ccgaactccg cagcttcgat accattgttg ccactaaggt ggttgtggca
     5401 aacgaaaaac tttacgttaa ccgagccgaa ggctttgtgg gtacaggtct gaaaaaagac
     5461 gaatacgttt gcgactgctc cgatatcgac gatgtaatag taattctccg caatggcaag
     5521 tactacatcc gtaaggttca ggaaaaggac ttctttggta aggatattgt ccacgttgga
     5581 gtttataaga agaacgatac ccgaacaatt tacaatgtgg tttaccgcga tggcctaaac
     5641 ggcgatatca tgatgaagcg atgcgccatt aacggaacca cacgcgataa ggagtacgac
     5701 ataacaaagg ggacacaggg ttcacaaata ctctggcttt cggcaaaccc aaatggtgaa
     5761 accgaagtac tgaaggttta cctgaaacca aggccacgcc tccgtaacct tatacttgaa
     5821 ctggatttca gccaactggc agttaaagga cgtggctccc agggaaatat ctttacccga
     5881 tacgccatcc acaaaatcat gctcaaggaa aagattgctg gcgcagtgga gggtgtaaag
     5941 gtttggtacg atcctgaggt tatgcgcctg aaccacgagg gacaaggtga actgcttggc
     6001 gaattcttgc ccgatgaacg cgttctggtt gtgaaccccg atggtaccta tttccttgcc
     6061 aacaccaatt atgccctcag gttcgatgtg caacccctac tggttgaaaa gtttaacccc
     6121 cacaaggtat tctcggccgt ttatttcgat ggcgagcaaa aacagtacta ccttaaacgt
     6181 ttccgttttg aacccaccga gaaaacccaa tcctttatcc ccgacgagta tagctcaaaa
     6241 cttatagctt tgaatgccga cgtattcccg gctttgaaac ttgtttttgg aggcaaaaac
     6301 gcaaatcgcg aacccgagaa tatcgatgtt gatgccttta ttgccgtaaa gggctacaag
     6361 gcaaaaggca aacgtctaac cacgttcgat gtaaaacggg ttgagtttat tgaacccctg
     6421 gaaaaggaaa gatcagcttt tgaggaagaa acaagcggaa ttcctgattc agaatcggaa
     6481 gataacgatt caggttcttc agctgagcaa atgagcttgg aactttagca aaaaagagca
     6541 gtttttctgc tcttttttta tatctttgat ctcccttaat tttttttata aaatattgtg
     6601 aattagacat tttgcattaa acttaaataa agcattaagc gatgaagatt tttctgattg
     6661 gattcatgtc gagcggaaaa accacagttg gaagggagtt ggctcatttc ctaagttacc
     6721 aattcctcga tttggatgag tatattgaaa ccaagcacaa tagaacaatt aaacaaattt
     6781 ttgaaacaaa aggcgaagag cacttccggc ttattgaaaa cgaagcactt aaagaggttt
     6841 gttcatttaa cggcgatttt gtgatttcat cgggtggtgg aacatcgtgc ttttacaaca
     6901 atatcgacta catgaaccga aatggcatta ctgtttacct taggcttgag gtttcaaccc
     6961 tggtttcgcg tttggtggaa tcaaaaatcg ataggccttt actttggggt aaaactcgcg
     7021 aggagctgaa cgattacatt atacgtgttc ttgacgaacg aaaaaaattt tacgagaaag
     7081 ctcaaatcat tgtggatgcc gataatacca agccaatcga tttggcgaaa ttaataatat
     7141 cagcacttga gtagataggc atattagcat tttcggatgc aatagacata gttttactaa
     7201 aacttttagg ccattcctct tgattttttc tttaaaatgc ttatgtttgc aataatttga
     7261 aacagaatga aggcgatgcg atacattttg ggtggattgt gcttagtact aacttttaac
     7321 actccagtat ttgctcaggt tgagaaccag agtttaaatt ccgactccat ttatgagcaa
     7381 agacaaataa accagatgga tagcctgctt taccagtggt atatccagca acccaatgaa
     7441 gataatagcc tggtagccga tattgagggc gacaccattg aagggcctga actccccgac
     7501 tcattttaca taaagcgtct gcaaaccatc aactcattaa ttgatttacc ctacaataat
     7561 attgttagga g
//
LOCUS       DAOOWJ010000118         7542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_9763_length_7542_cov_7.512355, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000118 DAOOWJ010000000
VERSION     DAOOWJ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9763_length_7542_cov_7.512355"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..905
                     /locus_tag="PLW22_10090"
     CDS             111..905
                     /locus_tag="PLW22_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOU75093.1"
                     /translation="MEGNAVIPVLENVWEKSTDPVLQSRIENIIDSIQYNYLAGEFQK
                     WAQSPEMDLLQGTILVAKIQYPELNPSPIHEELEKIRRDIWLELNSSLTALEKVKIIN
                     HVIFTLHGYKPDTSNYFAPKNYFINTLIETKQGGPIILSLFYAIIAQKLGLPIYCVNL
                     PHNFVLGYKDRYYQKTSDYPAEEKRSILFYINPFNQGSLFGAKEIELFLKQQKIEEEI
                     NFYLPCSNKESILQLIYNASVALQRSNRQNKAILFNRIHDLLADKD"
     gene            complement(917..1150)
                     /locus_tag="PLW22_10095"
     CDS             complement(917..1150)
                     /locus_tag="PLW22_10095"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU75094.1"
                     /translation="MKKNEKIICHCNGVSEKAVVKTIHQKGAIGMNDIQRLTLAATGC
                     RRCFAQVEEILLSELDKLKVNGLQLKINFIEDK"
     gene            1230..1583
                     /gene="rpsF"
                     /locus_tag="PLW22_10100"
     CDS             1230..1583
                     /gene="rpsF"
                     /locus_tag="PLW22_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOU75095.1"
                     /translation="MLNQYETVFIATPVLSEAQMKETVTKFRDFITENGGEIVHEESW
                     GLRKLAYPIQKKTTGFYHLIEFKADGALIDKLETQYRRDERIIRFLTVKMDKHHVEFA
                     QKRRNAKVEPKTMEG"
     gene            1587..1856
                     /gene="rpsR"
                     /locus_tag="PLW22_10105"
     CDS             1587..1856
                     /gene="rpsR"
                     /locus_tag="PLW22_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOU75096.1"
                     /translation="MSQNQSEIRYLTPPSVEIKKKKYCRFRKNNIKYIDYKDPEFLKK
                     FLNEQGRILPRRITGTSQKYQRKVATAVKRARHLALLPFVTDLLK"
     gene            1876..2322
                     /gene="rplI"
                     /locus_tag="PLW22_10110"
     CDS             1876..2322
                     /gene="rplI"
                     /locus_tag="PLW22_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOU75097.1"
                     /translation="MEIILLQDVPNLGNKNDVVTVRNGYARNYLIPRGMAVTATESAK
                     KVIAENIKQQAHKEAKIKAEAEQLAKKLEGVKLTIGAKTSSTGKIFGSVNNIQIAEAL
                     AAKGFEIDRKSITLSEDQVKEVGTYKAEIKLHREVKVSIEFEVVSE"
     gene            complement(2390..2770)
                     /locus_tag="PLW22_10115"
     CDS             complement(2390..2770)
                     /locus_tag="PLW22_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU75098.1"
                     /translation="MEKKIICTEKAPKAVGPYSQAVEANGFLFISGQIPIDPSTGKVV
                     EGRIKEQTEQVLKNIGAILKEAGLDYKNVVKTTCLLSDMDNFAAMNEVYARYFTSEMP
                     ARAAYGVVRLPLGVMVEIECIAVR"
     gene            complement(2787..5372)
                     /locus_tag="PLW22_10120"
     CDS             complement(2787..5372)
                     /locus_tag="PLW22_10120"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOU75099.1"
                     /translation="MRFRFTLLLLLINQLALLANRFDSVALKQMLAYAKPDTVSTPDS
                     AKNKKSAIDDPVLTNADDSIVYSLDGKIAYLYGNGVVTYQNLELKANYIEFDMETKQV
                     YASGLPDSTGKISGKPKFKEGSQSFEMEKIYYNFDTRRAKIIGVVTEQSGGFMHSAVT
                     KKMEDDVVNLQDGKYTTCDLDHPHFYIAITKGKMIPDKKIVAGPAYLVIEDVPFPLII
                     PFGFFPNTRKRAAGVLIPEYGEENNRGLFLRNGGFYFGMGDYMDQRLTFDVYSRGSWG
                     VRGQTSYKLRYKFSGSLNLEYSTIVTSDKGLPDYSKQNSYWIRWSHNQDPKASPNSTF
                     QANVNFGSSNYNRYNARSINTALSNTFNSSISYSKTWPGSPFSLSTSLTHSQNTINKS
                     VDLNFPRVAFNMSRIYPFKRKNAIGVPKWYEKVGVSLNTSLENRVQVKEDNLFKRTVF
                     DSLQNGMKHSIPVSTSFNLLKFITVSPGVNYNEFWYLQTIEKYWNADSNRVEIDTLRG
                     FKRGYEYSTSVSMSTKVYGMFQFSKKSKVQAIRHVITPSVSLGYRPDFSDPKYGFYRT
                     VQSDSTGKTTRYSIFEKGIYGGPGGGKSGVVSFALSNLLEMKVKSANDTVNPVKKVKI
                     IDGLSMNASYNLLADSLNWSEVSVSGRTGLFDGKVNINYSGGFNLYAINEQGRIYNRY
                     EWNESGKLARFTRGNIGVDFSLNSKKDGQANSSTAGRVGGAGEIPPGTAPDGSNFGES
                     LGTMYGLDYVDFSVPWNVRFSYNLSYSKPALQSSLVQTLSFSGSISLTPKLKIGFNSG
                     YDFKNKKLTTTSLNFYRDLHCWEMRLTVIPIGYYKSFSFQINVKSSILQDLKFTKRDH
                     YLDNQ"
     gene            5485..6597
                     /locus_tag="PLW22_10125"
     CDS             5485..6597
                     /locus_tag="PLW22_10125"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOU75100.1"
                     /translation="MALLIISLFSANTTKSQHLSAYSVSKIVIDAGHGGKDPGTVGKI
                     SKEKDITLSIAKRVGELINQNLPGVKVVYTRSDDTFIPLDERSRIANRESADLFISIH
                     CNAAPNKGVTGTETYVMGLHKSNENLDVAMRENAVITYEDEYHEKYEGYDPNSAESFI
                     IFTMLQNAFLEQSLDFASIIQGDLKERAKRPDRGVRQAGFLVLWKTSMPSVLVEVGYL
                     SNLKEEVFLNSNEGQELIASSIYRAIKAYKQKIDAIVESNQKTVKPDSSKPENIKNES
                     TTMAKVNTIEFKVQIASSQTPINSKGTQFNGINDIDTLVVNGVYKYLVGGSQSYPEAL
                     EYCKVVKQKFPDAFVVAIENGEIIPIKKALEKINSN"
     gene            6657..>7542
                     /locus_tag="PLW22_10130"
     CDS             6657..>7542
                     /locus_tag="PLW22_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOU75101.1"
                     /translation="MKLKLTRETKIGLFTLTILVVMFWGVNFLKGKNIFSPNNVYYAI
                     FKSVDGLKNTDAVLINGFKVGLVKNIEFHDLKSGRFRVVLLVNKKYSLPRNTTAKLVS
                     TDIMGGKAIKLEVAPDSLFYQPGDTLPTSIEVGLLDQLAYQMSPVKEKAEVMMSEIAK
                     TLVVLQEVLNDENKKNLSEGIENLNRTLHNIANLSAGLDTMVNSPKGSIRLSLSNIES
                     ISSNIRKNNADITNTIKNLSNLSDSLSKANLYSTLVKLDSSLIQLQQALTKVSSGEGT
                     LGKLVYNDSLYRNMESASL"
BASE COUNT         2349 a         1605 c         1492 g         2096 t
ORIGIN      
        1 tagcaacatt agagtagagc atattggatg ccaagtacat aaaatcgctc atttccctcc
       61 tcgatgaccc tgataccaat gtgtttaaca cggtatcaga aaaaatcatc atggagggga
      121 acgcggttat tcctgttctg gagaacgttt gggagaaatc aaccgaccct gtactgcaaa
      181 gcaggattga gaacatcatt gattccattc agtacaatta cctggcaggg gaatttcaaa
      241 aatgggcaca atcgcccgaa atggacttac tccaagggac aatacttgtt gccaaaatcc
      301 aataccccga acttaacccc tctcccattc acgaagaatt agaaaagatc aggcgcgaca
      361 tttggcttga acttaacagt agccttactg cactggagaa agtaaaaatc atcaaccacg
      421 ttattttcac attacatggc tataaacccg atacatcaaa ctactttgct cccaaaaact
      481 attttattaa caccctaata gaaacgaaac agggcggacc tattatacta tctctcttct
      541 atgcgattat tgcgcaaaag cttggcttac ccatttactg tgttaaccta ccccacaatt
      601 ttgtgcttgg atataaggat aggtattacc aaaaaaccag cgactatccg gctgaggaaa
      661 aaagaagcat tctcttttac atcaaccctt ttaaccaagg atcgcttttc ggtgctaaag
      721 agattgaact gtttctaaag caacaaaaga ttgaggaaga gataaacttc tacctgccat
      781 gcagcaataa ggaatcaatt ctgcagctaa tctataacgc atcggtagcc ttgcaacgtt
      841 caaaccgaca gaataaagcc atccttttca atagaattca cgatttactg gccgacaaag
      901 attaggagca taaacatcat ttgtcttcga taaagttgat tttcagctgt aatccattta
      961 ccttgagttt atccaactcg gaaagaagaa tttcttcaac ctgagcaaaa catcttcggc
     1021 atccagtggc agccaaagtt agtctttgga tatcattcat cccaattgcg cccttttgat
     1081 gaattgtttt aacgacagcc ttttccgaaa cgccattgca atggcaaatg attttttcgt
     1141 tttttttcat gggaatggat taaaaatgtt ttgcagttac aagtaaaagg cttaaatttg
     1201 cgcgctaatt taaataaaat caattaacta tgttaaatca gtacgaaacc gttttcattg
     1261 caactcccgt tttgtctgag gcccagatga aggaaacggt taccaagttc agggatttca
     1321 tcactgagaa tggtggtgaa attgtccatg aagagagttg gggattaaga aagttggcct
     1381 accccatcca gaaaaagacc accggttttt accacctcat tgagtttaaa gccgatggcg
     1441 cattgattga taagctggaa acccagtacc gtcgtgatga gcgtattatc cgcttcctaa
     1501 ccgtgaagat ggataagcat catgttgagt ttgctcagaa aagacgcaat gccaaggttg
     1561 aacctaaaac tatggagggt taaacaatga gccagaatca atcagaaatc agatatctca
     1621 cgcccccaag tgttgagatt aaaaagaaaa agtactgccg tttccgcaag aacaacatca
     1681 agtacattga ctacaaggat cctgagttcc tcaaaaagtt cttgaacgaa cagggaagaa
     1741 ttctacctcg tcgtattact ggaacttcgc agaagtatca gcgtaaggta gcaactgccg
     1801 ttaaaagggc acgccacctg gcattgcttc catttgtaac cgatttatta aagtaacagg
     1861 taggaggaca agcacatgga aatcatacta cttcaggatg ttcccaattt gggtaataag
     1921 aacgatgttg ttaccgttcg taacggctac gctcgcaact acctgatccc aagaggaatg
     1981 gctgtaaccg ctaccgaatc ggccaagaag gtaattgctg aaaacattaa acagcaagcc
     2041 cataaggaag caaaaatcaa agccgaagct gaacaacttg ccaagaaact tgagggcgtt
     2101 aagcttacca ttggtgccaa gacaagctca accggtaaga tttttggatc agtaaataat
     2161 atccaaatag ctgaggcttt agcagcaaaa ggcttcgaaa tcgatcgcaa gagcattacg
     2221 ctatccgaag atcaggttaa agaggttggt acctataagg ccgaaatcaa acttcatcgc
     2281 gaagttaagg ttagcattga gttcgaagtt gtttccgagt aaactaattg aaccaaataa
     2341 acaaaaggct gcttataata gcagcctttt gtttttggtg attatcgggt tagcgaactg
     2401 ctatacattc aatttcaacc atcacaccta agggtagtcg aactacacca taagcagccc
     2461 tagcaggcat ttcggatgtg aagtagcgag cgtaaacctc gttcatggca gcaaagttat
     2521 ccatatcgct cagcaggcag gtggttttaa caacattctt atagtcgagg cctgcctcct
     2581 tgagaattgc tccaatattc ttcaaaacct gttcggtttg ctctttaatt ctcccttcaa
     2641 ctaccttgcc ggttgaggga tcgatgggta tttgccctga aatgaacaag aaaccgtttg
     2701 cttcaacagc ctggctgtaa ggccctacag ccttaggtgc cttttcggta caaattattt
     2761 tcttttccat ggttattttg gtttggttat tggttatcga gatagtgatc gcgcttggta
     2821 aacttaaggt cttgtagaat tgacgacttt acgtttatct ggaaactaaa actcttatag
     2881 tagccaatgg gaattacagt taagcgcatt tcccagcaat gcaagtcgcg ataaaagtta
     2941 agcgaagtgg ttgtaagctt tttgtttttg aagtcgtagc ctgagttaaa accaatcttc
     3001 aactttggag taaggctaat gcttcctgaa aagcttaaag tttgcactaa cgatgactgc
     3061 aatgcaggtt tactatagct taggttgtag ctaaacctca cattccaggg aaccgagaaa
     3121 tcgacataat caagtccgta cattgttcca agcgattcgc caaagtttga gccatcggga
     3181 gccgttcccg gaggtatctc acccgctccc ccaactcttc ctgcggtgga tgaattggcc
     3241 tgcccatcct ttttgctatt caatgagaaa tcgacgccta tattccctct ggtaaacctt
     3301 gccagcttac cgctctcgtt ccattcatac ctattgtaaa ttcgcccttg ctcatttatg
     3361 gcatacaggt taaaaccgcc agaatagttg atgtttacct tcccatcaaa caggcctgtt
     3421 ctaccgctaa ccgacacctc agaccagttc aaagaatcgg ctaaaaggtt atatgaggcg
     3481 ttcattgaca atccatcaat aatttttact tttttaaccg ggttaaccgt atcgtttgcc
     3541 gatttaacct tcatttccag caggtttgaa agtgcgaaag aaactacccc cgatttgcct
     3601 ccacctggtc ccccataaat acctttctca aaaattgaat acctggttgt tttaccagtg
     3661 gaatcggatt gaactgtcct ataaaaaccg tatttgggat cagaaaaatc ggggcgatag
     3721 cctaaggaaa ccgaaggggt aatcacatgc cttattgctt gcacttttga tttcttagag
     3781 aactgaaaca ttccataaac cttggtgctc attgaaaccg atgtgctgta ctcataaccc
     3841 cttttaaacc cacgcagtgt atcaatctca accctgttag agtcagcatt ccaatacttc
     3901 tcaattgttt gcaagtacca aaactcgttg tagttaactc cggggctaac agttatgaac
     3961 tttaaaaggt tgaacgaggt agaaacagga attgaatgtt tcattccatt ttgcagacta
     4021 tcgaatactg tacgtttaaa tagattatct tcctttacct gaaccctgtt ttccaggcta
     4081 gtatttaggc taaccccaac cttctcgtac catttgggta ccccaattgc atttttccgt
     4141 ttaaaagggt aaatcctgct catattaaaa gccaccctcg gaaaattcag atcaaccgat
     4201 ttatttattg tgttttgaga gtgtgtcaac gaagttgata agctaaatgg ggagccaggc
     4261 caggtttttg aatacgaaat acttgagtta aacgtatttg agagcgcagt atttatacta
     4321 cgggcgttat acctgttata gttcgatgaa ccaaagttta catttgcctg aaaagttgaa
     4381 tttggcgaag cttttgggtc ctgattatgt gaccaacgaa tccagtacga gttctgttta
     4441 ctgtaatcgg gtaaaccttt atcgcttgtt acaatagtag aatattctaa atttaaagat
     4501 cctgaaaact tatacctgag tttatatgat gtttgccctc gtacacccca cgaccccctt
     4561 gagtatacat caaaagttaa acgttggtcc atgtaatcgc ccatgccgaa gtaaaatcca
     4621 ccatttctaa gaaatagtcc tctgttattc tcctcaccgt attcgggtat taaaacgcca
     4681 gctgcgcgtt tacgggtgtt agggaaaaaa ccaaacggaa taatgagggg aaaaggtaca
     4741 tcctcaatga caaggtatgc aggaccagca acaatcttct tatcgggtat catcttaccc
     4801 tttgtaattg caatgtaaaa gtgaggatgg tctaaatcgc aggtggtata cttaccatcc
     4861 tgaaggttta ccacatcgtc ctccatcttt tttgttactg cgctatgcat gaagccgcct
     4921 gactgttcag ttaccacacc tataatttta gccctacgtg tatcaaagtt gtagtaaatc
     4981 ttttccattt caaacgactg gcttccctcc ttaaactttg gtttaccgct aatttttccg
     5041 gtactatcgg gtaatccgct tgcgtaaacc tgcttggttt ccatgtcaaa ctcaatgtag
     5101 ttagccttca gctcaagatt ttgataggta accacaccat taccatacag atatgcaatt
     5161 ttaccatcga gggagtatac aattgaatcg tcggcgttgg taagtactgg atcgtcgatg
     5221 gcactttttt tgttcttagc actatcggga gttgaaacgg tatcgggttt ggcgtaagcc
     5281 aacatctgtt ttaaggccac agagtcgaac ctgttagcaa gtaatgccaa ctggtttata
     5341 agtaaaagca gtaaagtaaa cctaaatctc aactttaatc ctatttttgt gaacaaattg
     5401 agctcaaaac tacttaaaaa aataaaagaa tacgcaaggg gtatgaggcg ggaatataaa
     5461 tttttatcaa catttaactt ttatttggca ttactaatta tttcactttt ttcagccaat
     5521 acaactaaat cgcagcacct aagcgcatac agtgtatcaa aaattgttat cgatgcgggt
     5581 catggaggca aggatcctgg aacagttggc aaaatcagca aggaaaagga cataaccctt
     5641 tccattgcca agagggttgg agagttaatt aaccagaact taccgggcgt gaaagtggtt
     5701 tacacccgat ccgatgatac ctttataccc ctagacgaac gtagcagaat tgctaaccgt
     5761 gaaagcgccg atttgtttat atcaatccat tgcaatgctg cacccaataa gggtgttacc
     5821 ggaaccgaaa cctacgttat ggggttgcat aagagcaacg agaaccttga tgtagcgatg
     5881 cgcgagaatg ctgttattac ctatgaggat gaataccacg agaaatatga aggttatgac
     5941 cctaactccg ccgaatcgtt cataattttt accatgcttc aaaatgcttt cctggagcaa
     6001 agcctcgatt ttgcctcaat tatccaaggt gatttaaagg aacgtgctaa gcgtcccgat
     6061 agaggtgtta ggcaggcagg ctttcttgtg ctttggaaaa catcaatgcc aagtgttctt
     6121 gtggaggttg gctaccttag caatctaaaa gaggaggtgt ttttgaattc aaatgaaggg
     6181 caagaactta ttgcttcatc aatttacaga gccattaaag cctacaagca aaaaattgac
     6241 gcaattgtag aatcaaacca gaaaacggta aagcccgact catcaaaacc agaaaatata
     6301 aagaatgaat cgacaacaat ggcaaaagta aatacaatag agtttaaggt tcaaatagcc
     6361 tcatcacaaa ccccaatcaa cagtaaagga actcagttta atggcataaa tgatattgac
     6421 acccttgtgg ttaatggagt ttacaaatac ttggttgggg gttcgcagtc gtaccctgaa
     6481 gcactggaat actgcaaggt tgttaaacaa aagtttcctg atgcctttgt agtagccatt
     6541 gaaaacggag agattattcc cattaaaaaa gccttagaga agattaactc aaactagatg
     6601 cctactttcc caataattga gtaattttga aatgtaaaca accaaaagaa ccagccatga
     6661 aactcaaact aacccgcgaa acaaaaattg gcctgtttac ccttaccata cttgttgtaa
     6721 tgttctgggg agtaaacttt ttaaagggta aaaatatttt tagcccaaac aatgtttact
     6781 acgccatttt caaatcggtt gatgggttaa aaaataccga tgccgtgcta attaacggct
     6841 ttaaggttgg cctggttaaa aacatagagt ttcacgactt aaagtcgggg aggtttcggg
     6901 tggtattact ggtaaataag aagtacagtc tcccccgaaa cactactgcc aaactagtaa
     6961 gcaccgatat aatgggaggt aaggccataa agctcgaggt agcccccgat agcctgttct
     7021 accaacctgg cgacactctt cctacatcca ttgaggttgg actcctcgat caacttgcct
     7081 accaaatgtc gcccgtaaag gaaaaagcag aggtaatgat gagcgagatt gctaaaaccc
     7141 ttgttgtact tcaggaagtg cttaacgatg aaaataaaaa gaacctgtcg gagggtattg
     7201 agaacctcaa tagaaccctc cataacattg caaacctgag tgctggcctt gacaccatgg
     7261 ttaacagccc aaaaggttcc atcaggttat cgctaagcaa tatcgagtca atttcatcaa
     7321 atatccgaaa gaacaatgcg gacattacaa acactattaa aaacctttca aacttaagcg
     7381 attcactatc taaggctaac ctttacagca cgcttgtaaa gttagactcc tcgctaatcc
     7441 agctccaaca agctttaaca aaagtaagtt caggtgaggg cacattgggc aaactggttt
     7501 acaatgactc gctataccgt aacatggaat cggcatcatt ac
//
LOCUS       DAOOWJ010000119         7468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_9906_length_7468_cov_4.167948, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000119 DAOOWJ010000000
VERSION     DAOOWJ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9906_length_7468_cov_4.167948"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1514
                     /locus_tag="PLW22_10135"
     CDS             3..1514
                     /locus_tag="PLW22_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOU75102.1"
                     /translation="MAIALLENKQLVELNKERSNVQFSVGDIYLGKVKKIMPGLNAAF
                     VDVGYEKDAFLHYLDLGPQFKTLQKYVQDHYSRKNKTIPISKYNMLPDIDKGGKITDV
                     LSSGQPVLVQIAKEPISTKGPRLTSEISIAGRNIVLVPFNDKVSISQKITAPEERKRL
                     KRLMESILPRNFGVIVRTAAEGKKVAVLDAELRSLIKRWEITVEKLKNPGYPTLLTSE
                     IKRTSAILRDMLNGSFNSIHVNDENVYEEILEYIESIAPEKEKIVKLYTGNVPIFDQF
                     GVEKQIKSFFGRTVSIKSGAYLIIEHTEALHVIDVNSGNRSKSSNDQETNALEVNLAA
                     TDEIARQLRLRDMGGIIVVDFIDMHNNENRQMVYERMKELMSRDRAKHNILPLTKFGL
                     MQITRQRVRPEMHIDTSEKCPVCNGSGKVTATILLDEQIENQLSLICATQKYRFVTIK
                     LHPYIAAYIKKGFPSKRFKWALKFKCAVNLLPMSSYHFLEYHFFDKNDNEIEL"
     gene            1669..2184
                     /locus_tag="PLW22_10140"
     CDS             1669..2184
                     /locus_tag="PLW22_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75103.1"
                     /translation="MLASLLAVEVESCENDESSDYDNSLTISGSVTIDGKTYSNPTFN
                     MDTSMEVEGYTYLTYNPTAQIYYTAIYPKTEMIDAGSGYNVHYYLVLEDNKPGTASLT
                     GMIIFHKNDNLVLFLESKDVQTTIDKIDPIGGFIEATYSGTFSDNSNTPKTYTVTGKI
                     KSKRVEEPTKK"
     gene            complement(2210..4465)
                     /locus_tag="PLW22_10145"
     CDS             complement(2210..4465)
                     /locus_tag="PLW22_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOU75104.1"
                     /translation="MRNVLLAIVVLTAFLSCNKTNEVNLIPKPVSFKLRDGSFTISGK
                     TKVKVDSTDSLAFENAAYLVELFNSKGVSCALESAINDDLLNCIIFTRTSCPDSLGAE
                     GYMIDVNPKAITIRANQSAGFFYAIQTLRQLMPANFEKNDFRAKSLTIPYVVIVDKPR
                     FSWRGLNLDCCRHFMNKDFVKRYIDLLAYHKMNVLHWHLTEDQGWRIEIKKYPKVTEV
                     GAWRTNTDGTVYGGFYTQDDIREVVAYAAKRHVMVVPEIEMPGHSVAALAAYPHLSCT
                     GEPLKVETNWGVFKDIYCAGNDSTFIFLQDVLSEVIELFPSPYIHIGGDEAPKYRWEN
                     CPKCRARLKTEKLSNYEELQRYFITRISKFLETKGKTIIGWDEILEGGRPHNAMVQAW
                     RGIDRASEALKANAPVVVSPTSHCYFDYPVEVTNLQKVYSFDPVPDKADSAKVPLVLG
                     GECNMWTEYAPQETVDSKVFPRLLAISEVLWTYSNQREYPEFLKRVRYHYNRLDALGV
                     SYGFEQSAVEFKSRIGDDRKSMAVELVKGQDNLEVRYTTNGDEPTEKSTPYRKPILLT
                     SPTTLKAAAFNNGYRVGDVFERAFMVSKSLGKPIELGFKPSEKYMGGGLQALVDGRLG
                     TPKYNDGIWQAVQGEDIVVTVDLGSEQPINSVAIGFLQNIPSWIFLPGCVDVFVSADN
                     STFERIAQLDCPDSIKQQPMLIYRFNASKVKEIKGRFVRFVAHNPGPCPDWHPASGSP
                     TWLFADEVVVE"
     gene            complement(4478..7426)
                     /locus_tag="PLW22_10150"
     CDS             complement(4478..7426)
                     /locus_tag="PLW22_10150"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOU75105.1"
                     /translation="MKPLVLVTVLMQAINLWAQNGPADYVNPFIGTDFHGHTYPGATV
                     PFGMVQLSPDTRLTGWDGCSGYHYSDRVIYGFTHTHLSGTGASDYGDILFAPVTGKSP
                     VFENSKYSSPFNKKNEQAKAGYYSVVLDKYKVKAELTTTVRVGFHRYTYAKPNEPAMV
                     IDLSHRDKVLEAWFKVTGKNELRGFRRSSQWAKDQKIYFVARFSEPFVAGPKFTDDSG
                     EMSSLSNEDGTIEVHGTKLKAWVQFPKLKNKVLLVKVGISFVSEEGALKNLEAEVPGW
                     DFDGVVEAARKQWNEELSKIQVEGGVPEELTTFYTAMYHAMVVPNIFSDVDGLYRTMD
                     GNIHSAEGFTPYTVFSIWDTYRAWHPLMTIIDTKRTSDYINTFLSHYRYGGRLPVWEL
                     AANETDCMIGYHSVSVITDAWVKGIRGFDANYAMQAMKHSANLNHFGLQFYKHFGFIP
                     GDMEHESVSKTLEYAYDDWCIAQMAKGLGKDDDYISFIKRAQSYKNLFDPETRLMRPR
                     LNGGFKHNFNPAEVDQHFTEANSWQYSFYVPHDVTGLIYLHGGMEKFEAKLDELFNAP
                     SQLAGRNQADITGLIGQYAHGNEPSHHMAFLYNYVGKPWKTQQLVKRIMNEMYSHKPD
                     GICGNEDCGQMSAWYILSAMGFYPLCPGSTQYAIGSPLFPKITINLENGKKFTVIAEN
                     VNKQNIYIQKATLNGNAISRSWIDHAEIMAGGELVLIMSSTPNETWGTQPADLPSTSI
                     DDNPITVAPFLVAASDVFTDTLTVSISSVQPDVQIFYAVEPIDKPLPQTSWIQGDRVN
                     VTESRKVRAFAILPNGQTSAILEGVYRKVNSIANVQLKSKYSSLYTAGGDLGLVDGVR
                     GSKNFRIGGWQGYQGQDFEAIVDLGELKPVRQIAAGFLQDASPWIMMPREVVFSVSTD
                     GQNFIVVGSVKSDIPESQMEPTIKDFTVDVDTKARFVKVFAKAFGPLPSWHESAGEPS
                     WLFIDEITVK"
BASE COUNT         2139 a         1775 c         1545 g         2009 t
ORIGIN      
        1 aaattgcgat tgccctgctc gaaaataagc agctggtaga gctcaataag gagcgaagca
       61 atgttcagtt ctcggttggc gacatctatc tcggtaaagt taaaaagatt atgcccgggt
      121 tgaatgcagc ttttgtagat gtaggctatg aaaaagatgc atttcttcat taccttgacc
      181 tgggcccaca gtttaaaaca ctccagaaat acgtacagga tcactactcg cgcaaaaaca
      241 aaaccattcc catttcaaag tacaacatgc ttcccgatat cgacaagggc gggaagataa
      301 cggatgtgct tagttcggga caacctgtac tggtgcaaat tgccaaggaa cccatttcga
      361 ccaaagggcc aaggcttacc tccgaaattt ccattgcagg aaggaacatt gttcttgttc
      421 cctttaacga caaggtttca atctcgcaaa aaattacagc ccccgaggaa cgtaagcgcc
      481 tcaaaaggtt gatggaaagt attttaccaa gaaattttgg agttattgtt cgcactgccg
      541 ctgaaggtaa aaaggttgcc gtactcgatg cagaactgcg aagcttaatt aaacgatggg
      601 aaataacagt tgaaaaactt aaaaaccctg gctatcctac cctcttgaca agcgaaatca
      661 aacggacatc ggctatcctg agggatatgc ttaacggttc ttttaacagc attcacgtaa
      721 acgacgagaa tgtttacgaa gaaatactcg agtacatcga aagcattgca cctgaaaagg
      781 aaaagattgt aaagctctac accggtaatg tgccaatatt cgaccaattt ggcgttgaaa
      841 agcagatcaa atcgttcttt ggccgaaccg tttccattaa gagtggtgca taccttatta
      901 ttgagcatac cgaggctttg catgtaattg atgttaacag cggcaacagg tcaaagtcgt
      961 cgaacgacca ggaaacaaat gcccttgagg taaacctggc tgcaactgac gagattgccc
     1021 gacagctgag gttgcgcgac atgggtggaa taattgttgt ggatttcatc gacatgcaca
     1081 acaatgagaa taggcaaatg gtatatgagc gcatgaagga gctcatgagc cgcgaccgtg
     1141 ccaagcacaa tattttgccg ctaaccaagt tcggattgat gcagattacc cgtcaacggg
     1201 tacgtcccga aatgcatatc gacaccagcg agaaatgtcc ggtttgcaac ggcagcggca
     1261 aggttactgc caccatcctg ctcgacgagc agatagagaa ccaactatcg ttgatttgtg
     1321 ccacccaaaa gtatcgcttt gtaaccatta agctacatcc atatattgct gcatatatca
     1381 aaaaggggtt cccttcaaaa cgattcaaat gggcattaaa gtttaaatgc gccgttaacc
     1441 tattgcccat gtcgtcgtac cattttctgg aataccattt cttcgataaa aacgataacg
     1501 agatagaact ttagccaacc aagtggggtg tcagggcatg acacccctta tttatattaa
     1561 aaataatgca attgttcaaa atattttata aacttttgcc atatattctg taaatttatt
     1621 ttgtaaacca aactcttcaa tctatctata aaacgcatat catacatttt gcttgcaagc
     1681 cttttggctg ttgaagtgga atcgtgtgag aacgatgaga gtagcgatta cgataactcc
     1741 ctaaccatta gtggcagcgt aacaattgat ggtaaaacct actccaaccc aacctttaac
     1801 atggatacat ccatggaagt ggaaggttat acgtatttaa cctataaccc taccgctcaa
     1861 atctattaca cagccatcta ccccaaaacg gagatgattg atgccggtag cgggtacaat
     1921 gtacattact acctggtact tgaagataat aaacccggta ctgctagtct tactggaatg
     1981 attatattcc ataaaaatga taatcttgtc cttttccttg agtcaaaaga tgttcaaaca
     2041 accattgaca agattgaccc aattggtgga ttcattgagg ctacttatag cggaactttt
     2101 tccgacaata gcaatactcc aaaaacatac actgtaaccg gaaaaattaa atccaaacgg
     2161 gttgaagaac ccactaaaaa ataaaagaag ccggtgcaaa ccgacttttt tattccacca
     2221 ctacctcatc ggcaaaaagc catgtaggtg agccggaggc aggatgccag tcggggcaag
     2281 gcccgggatt gtgagcaaca aaacgtacaa aacgcccttt aatttccttt acttttgaag
     2341 cattaaacct ataaatgagc atcggttgct gctttatact atccggacaa tccagctgag
     2401 cgattcgctc aaaagtagaa ttatcagccg aaacaaacac atctacacag cctggcaaaa
     2461 atatccatga tgggatattc tgaagaaaac caatggcaac gctattgatt ggctgctcac
     2521 tgccaaggtc aacggtaact actatatcct cgccttgcac ggcttgccaa atgccatcgt
     2581 tgtacttggg cgttcccaac cgaccgtcca ccaaggcttg caaacctcca cccatgtact
     2641 tttcagatgg cttaaagcca agttcaatgg gcttacctaa acttttcgat accataaatg
     2701 ccctttcaaa aacatcaccc actcgatagc cattgttaaa tgctgccgcc tttaaggtag
     2761 ttggagaagt taacaaaatt ggtttacgat agggtgtgct tttctcggtg ggttcatcgc
     2821 cgttggtggt gtaacgtacc tcaaggttat cctgcccttt aaccagctca acggccatgg
     2881 attttctatc atctccgatt ctcgacttaa actcaactgc cgattgctca aacccatagc
     2941 ttactcctaa agcatcgagg cggttgtaat ggtagcgaac acgcttcaga aattcaggat
     3001 attcacgttg attcgaatat gtccacaaaa cctccgatat ggcaagcaag cgtgggaata
     3061 cctttgaatc gactgtctcc tgaggggcat actcagtcca catgttgcac tctccgccaa
     3121 gtaccagcgg aaccttcgct gagtcggctt tatcgggtac agggtcaaag ctgtaaactt
     3181 tttgaaggtt agtaacctca acgggataat cgaagtaaca atggcttgtt ggcgacacta
     3241 caactggtgc gttagcctta agggcttccg aagccctatc gatacctcgc caagcctgaa
     3301 ccatagcatt atgcggacgc ccaccctcca aaatctcatc ccaaccaatt atggttttcc
     3361 ctttagtttc aaggaatttg gatatacgag taataaaata acgctgcaac tcctcataat
     3421 tactaagttt ttcggtttta agtcgtgccc tacacttagg gcaattctcc caacggtact
     3481 ttggtgcctc atcgccacct atatggatat atggcgatgg aaaaagttcg ataacctctg
     3541 ataaaacatc ttgcaaaaag ataaaggtag agtcatttcc cgcgcaatag atatctttaa
     3601 aaactcccca atttgtttct acttttaaag gttcacccgt acacgaaaga tgtgggtaag
     3661 cagctaaagc agccaccgaa tgaccgggca tctcgatttc aggaacaacc attacatggc
     3721 gttttgcggc ataagcaacc acctcacgaa tatcgtcctg tgtataaaag ccaccgtaaa
     3781 cggtaccatc ggtattggtg cgccatgctc ccacctctgt taccttagga tacttcttta
     3841 tctcgattcg ccaaccctgg tcttcggtaa gatgccagtg cagcacattc attttatggt
     3901 atgccagcaa atcgatgtag cgtttaacaa agtccttatt catgaaatga cggcagcagt
     3961 caagatttag ccctctccac gaaaaacgcg gtttatcaac aataacaaca taaggtatgg
     4021 ttaacgattt tgccctaaaa tcattttttt cgaaattggc aggcatcagc tgcctaaggg
     4081 tttgaatggc atagaaaaag ccagcggatt gattagccct aatagtaatg gctttgggat
     4141 taacatcaat catatagccc tctgcaccta agctatcggg gcaactggtt cgtgtaaaaa
     4201 tgatacaatt gagcaaatca tcgttaattg ctgattccaa tgcgcagctc acccctttgc
     4261 tgttaaacag ttcaacaagg taggcagcat tttcaaaggc caaactatcg gtactatcaa
     4321 cctttacttt ggttttgcca ctaattgtaa agctaccatc acgcagctta aagctgacgg
     4381 gtttaggaat tagattaacc tcgttggttt tgttgcacga taaaaacgct gtaagcacta
     4441 cgattgccaa taatacattt cgcatggctt aaagttttta ctttacggtg atttcatcga
     4501 taaaaagcca cgagggttcg ccggcactct cgtgccacga tggtagaggg ccaaaagcct
     4561 tggcaaacac ctttacaaat cgggctttag tgtcaacatc cacagtaaag tcctttatgg
     4621 ttggctccat ttggctttca ggaatatcac ttttaacaga cccaactaca atgaagtttt
     4681 gcccatcggt cgataccgaa aaaactacct cgcgaggcat cattatccaa ggacttgcat
     4741 cctgcagaaa acctgctgcg atttgcctaa ctggttttag ctcgcctaag tcgacaatgg
     4801 cctcaaaatc ttgtccctga tagccctgcc atccgccaat cctaaaattt ttgcttcctc
     4861 tcaccccatc aaccaatccc aaatcgccac cggccgtgta gagtgagctg tattttgatt
     4921 tcagttgaac attagcaatg ctgtttacct ttctgtatac cccttccaaa atggcacttg
     4981 tttgcccgtt gggcaaaatg gcaaaggcac gaacctttcg actctcggta acattaaccc
     5041 tatcgccctg aatccagctg gtttgaggca aaggtttatc tattggctca accgcataga
     5101 aaatttgaac atcgggctga accgatgata tggaaactgt taaggtatcg gtaaacacat
     5161 cagatgctgc aaccaaaaat ggcgctactg ttattggatt gtcatcaatt gaggtgctgg
     5221 gcaaatcagc aggctgtgta ccccaggttt cgtttggggt actggacatt attaaaacca
     5281 gttcaccacc ggccattatc tcagcgtggt caatccaact ccttgaaatg gcattcccat
     5341 taagcgtagc tttttgaatg taaatattct gtttgttgac attttcggca atcaccgtaa
     5401 acttcttgcc attctcaagg tttatagtga ttttggggaa tagcggtgaa ccaattgcat
     5461 actgggttga accgggacaa agcgggtaaa agcccatggc gctcaaaatg taccatgcgc
     5521 tcatctgtcc gcaatcctca ttgccacaga taccatcggg cttgtggcta tacatctcgt
     5581 tcattatccg tttaacaagc tgctgggttt tccaaggctt acccacgtaa ttgtaaagaa
     5641 aagccatgtg gtgactaggc tcattgccat gggcgtactg cccaatcaaa cctgtaatat
     5701 cggcctgatt acgtccggca agttgcgagg gcgcattaaa gagttcatcg agctttgcct
     5761 caaacttttc catccctcca tgtaggtata tcaaaccagt aacatcgtgt gggacataga
     5821 agctatactg ccagctattt gcctctgtaa aatgctggtc aacctcggca gggttgaaat
     5881 tgtgcttaaa acctccgttc aggcggggac gcattaaccg ggtttcgggg tcgaacaggt
     5941 tcttatacga ctgggcgcgt ttgataaaag agatgtagtc atcgtccttc cctaaacctt
     6001 tggccatttg ggcaatgcac cagtcgtcgt aggcatattc gagtgttttc gataccgatt
     6061 cgtgctccat atcgcccgga atgaacccga aatgcttgta gaattgcaac ccaaaatggt
     6121 tcaggtttgc gctatgtttc atggcctgca tcgcgtaatt ggcatcaaat cctcgaatgc
     6181 ccttaaccca tgcatcggta ataaccgata cggaatggta gccaatcatg caatcggttt
     6241 cgttggctgc aagttcccaa accggcaacc taccaccata gcggtagtgc gacaggaatg
     6301 tgttaatgta atcggatgtg cgcttggtat caataatggt catcaacgga tgccaagcac
     6361 ggtaggtatc ccaaatggaa aacaccgtgt acggtgtaaa accttcggca ctatgaatat
     6421 ttccatccat ggtacggtac aatccatcca catcggaaaa aatatttgga acaaccatgg
     6481 catggtacat ggcggtatag aacgtggtta actcctcagg tacccctcct tcaacctgaa
     6541 tttttgatag ctcctcgttc cactgcttgc gggctgcctc aaccacccca tcaaaatccc
     6601 agccgggaac ttcagcctca agattcttga gcgcaccctc ctcgctcaca aatgaaattc
     6661 ccactttaac cagtaaaact ttgtttttca actttgggaa ttgcacccat gctttcagtt
     6721 ttgttccgtg aacctcaatt gtaccatcct cgttgctaag cgaactcatt tcaccgctat
     6781 cgtcggtaaa ctttggcccg gcaacaaagg gctccgagaa tcgcgctaca aagtatattt
     6841 tttggtcttt agcccactgc gacgagcggc ggaaaccacg caattcgttt ttacccgtta
     6901 ccttaaacca tgcctccaac accttatcgc gatgcgaaag gtcaataacc atggctggtt
     6961 cgttgggttt agcataagtg tacctatgaa atcccaccct gaccgtggta gttagctcag
     7021 ccttcacctt atatttatcg agaactaccg agtagtaacc ggcttttgcc tgctcattct
     7081 ttttgttaaa cggcgatgag tacttgctat tctcaaaaac aggactttta ccggtgaccg
     7141 gtgcaaataa aatatcgcca tagtccgatg cccccgtacc gcttaggtgt gtgtgtgtga
     7201 atccgtaaat aaccctatcg gaatagtggt aacccgagca tccatcccat cccgttaatc
     7261 gtgtatcggg gcttagctga accattccaa agggaactgt agccccgggg taggtgtgac
     7321 cgtgaaaatc ggtaccaata aaagggttta cataatcggc aggaccattc tgcgcccaaa
     7381 ggtttattgc ctgcataagt accgttacta ataccagtgg tttcatacta tttaggttta
     7441 tgatttattc cgatgtttac ctttttat
//
LOCUS       DAOOWJ010000120         7347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_10140_length_7347_cov_7.930609, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000120 DAOOWJ010000000
VERSION     DAOOWJ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10140_length_7347_cov_7.930609"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            682..1641
                     /locus_tag="PLW22_10155"
     CDS             682..1641
                     /locus_tag="PLW22_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOU75106.1"
                     /translation="MDNRKLILIGLVVTAVCFFVLMLSFIPRPNSGFERYKSLNPWSD
                     SVYQSSLIFAIPIPENLSFAGEEVPLQYFDVRESLDRELHVNTFWHSQTVLLLKRANR
                     YFPIIEPILKKNNIPDDFKYLAVAESGLTQAVSPSKAVGFWQILEGTAKEYGLEINKE
                     VDERYHIEKSTEVACQYFKKAYEKYGTWTMAAASYNFGMNGITKQIDRQDSNSYYDMV
                     LGDETGRYVYRILALKVIFENPKQYGFFLDSTDLYPPLEYTEVKVDSTITSIASFARQ
                     YGVNYKLIKLFNPWLRENYLPGKQGKSYLIKIPRKGFREEAYK"
     gene            1650..4508
                     /gene="uvrA"
                     /locus_tag="PLW22_10160"
     CDS             1650..4508
                     /gene="uvrA"
                     /locus_tag="PLW22_10160"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU75107.1"
                     /translation="MFQDIDKSALTDEGEIVVEGARVHNLRNVDVTIPRQKLTVITGL
                     SGSGKSSLAFDTIYAEGQRRYMETMSAYARQFLGALERPDVDKISGLSPVISIEQKTT
                     SQNPRSTVGTITEIYDFLRLLYARASTAYSYQTGEEMVRYTDEQIINLINERFTGKRT
                     AILAPVVRGRKGHYKELFDQLRRKGYLNARIDGEITELRPAMKLDRYKTHYIELLVDK
                     FRVGEGDLKRLSQSVATAMNIGKGIIMVLDMDNGEYRYFSRNLMCPTTGISYDEPEPY
                     TFSFNSPKGACTHCNGLGYINQADIERIIPDPQLSIKRGGIQPLGPYKNSLIFWQLEA
                     IASKYDFSLSDPIGQIPDEALNVILYGSEEQFKLKHPSIGVNSSYFLSFEGVISYIQN
                     HQTNDELNGKGDRWANQFIKQVTCPTCKGTRLKEESLWFKVDGKSIADVSAMDIETLW
                     HWLQGLENRLGSRQKAIATEILKEIRERVRFLLDVGLGYLSLNRGSSTLSGGESQRIR
                     LATQIGSKLVNVLYILDEPSIGLHQRDNIKLINSLKQLRDAGNSVIVVEHDEEMIRSA
                     DYVVDIGPRAGRHGGKIVATGTPSEILMSNSLTAQYLNGQLKIPIPKERRKGNGKFLT
                     VKGARGNNLKNITVKFPLGTFICVTGVSGSGKSTLINETLYPALSRHLYRSYAQPLEH
                     DAIEGIENIDKVIDVDQSPIGRTPRSNPATYTNVFTDIRKLFELTPDAKIRGYKAGRF
                     SFNVKGGRCETCKGGGVQVIEMNFLPDVYVKCPDCFGRRYNRETLEVKYKGKNISEVL
                     DMTINQAVEFFDSIPQIHQKLKTLQDVGLGYITLGQQSTTLSGGEAQRVKLATELAKR
                     DTGKTLYILDEPTTGLHFDDVRILLEVLNKLVDRGNTVIVIEHNLDVIKSADYIVDLG
                     PEGGAAGGQVVCSGTPEDIAKSGCGYTSEFLTKYLFRE"
     gene            4566..4943
                     /locus_tag="PLW22_10165"
     CDS             4566..4943
                     /locus_tag="PLW22_10165"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU75108.1"
                     /translation="MDTLKIEKRIDSPMVLADGESGYFEVNGKSLPEDAIEFYKPLEK
                     YAQEYVKSPKKKTTINLKLEYLNTSSSKKLLDIIGYFEALPSQGYEVVLNWYHRDEDQ
                     DMIDEGIEFAHMTSLKVNFIVEQ"
     gene            4952..6853
                     /locus_tag="PLW22_10170"
     CDS             4952..6853
                     /locus_tag="PLW22_10170"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU75109.1"
                     /translation="MFRFVQYYVLLILFAFAHFTAQSQTTPLGSSKELQNSLQQAKEL
                     ESNGDLNGALSIYNKTATAYWVNGKLTEARDIFLKAASVAEKLGNQNALKVIYTNLGM
                     VFVDLEQYSEAIAQFEKSLAINRSQKNKAETTATLINIANAYKELDKYQKALDYAEQA
                     NTIALEINDAKLLRNTYSLLAEIHEALGNSEKSSEYFSLYTAFSRKIQREEQLKKEAE
                     AQKIVEEAKGELQVIKSEKELTEKELSEKQRVLETVKDSLDKIEQLTQEQKLQIDLLN
                     KENALREEKIKNQILVRNIFIVIIAATIGMLGLITHYYLQKKRSNEELARQKAMVEAK
                     SQELMQALQQIEKQNRDITSSINYAQRIQQALLPTYDQLVSYIPNAFIMFRPREIVSG
                     DFYWFAAYGGQNIAASKQNRHMIALPNIPKNDFGFLIAAVDCTGHGVPGAFMSMIGLN
                     LLDVIVRSGLTQPNLILNELHKQVRYLLKQENNDSRDGMDMALIRIKGDGRTIEFAGA
                     KNPIVYITNNQCFTIKGDPVPIGGLQKEEKREFTLHTITIDSPTSIYLFSDGYIDQFG
                     GPENLKFTSKRFREMLFENHNLPMVAQQEKLETALDEWIGSENNQIDDVLVIGLHLTG
                     KPFKIDSQT"
     gene            6903..>7347
                     /locus_tag="PLW22_10175"
     CDS             6903..>7347
                     /locus_tag="PLW22_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75110.1"
                     /translation="MSKQYFGVLVFLFLCSSVLAQENANNVYVNDNGDVFVKADAPIF
                     FFISPENQPSQKVLIPSTDKAANPMYFDGDGKHYLVYEGKGEKVRFLIWADGVGPKSS
                     LRVEKGLLFNHNNRVYVEDRASFTPSAIDSKSGVKQSFIAVDNQPF"
BASE COUNT         2266 a         1635 c         1633 g         1813 t
ORIGIN      
        1 ccaccgccac gttccacccc cgacccattg ccgggtcagg cttttatgcc tctgcctgct
       61 tcttccgctt cattccctct caggaataaa gtctgggatg aagccgtttt ccacaaacaa
      121 cccagatgac ccctctaaat ctcccctgag aggggagact ttagccctgc aataaggtct
      181 cctaattgct tccccctctc ctgtcaggag aggggtgggg tgaggtcgtt ttttacgaaa
      241 aacgaacaac gagttactag caacgcttcc agccccgcaa gggcgtaata tttgtaacac
      301 tatgattatt cccacatgta tagcgatgca cgtaatacat tttccaaaga gcaacactga
      361 aactttgcat cgcaacagaa cagcatggct aagagcagct tccaaaaaaa caacaaggat
      421 taatgctaaa gagatgcttc ggcgaagtat ttccctacaa atcgggactc agcacgacgg
      481 ggagctattt cggcagtaat tatcccgtaa attggaaatc aaaatgacaa aaatccctaa
      541 ttaaacggta ttggcggtat ttttgataaa tcaaaatatt gaaggggtat gccccttttg
      601 aagatcagcg ctaaattgta tttttgtaga ttaagttaac aaattggttc gggtaacgtt
      661 aaatggtaaa agttcattta catggataat agaaaactta ttttaatagg attggttgta
      721 actgccgtat gcttttttgt tttaatgcta tcgttcattc caaggcctaa ttcgggattt
      781 gagaggtata aatcgttaaa cccttggagc gactcggttt accaatcgtc gctaattttt
      841 gcaattccca tacctgagaa tttgagtttt gccggcgaag aagttcccct tcagtatttc
      901 gatgttcgcg aatcgcttga ccgtgaacta catgttaaca ccttctggca ctcgcaaacg
      961 gttcttctgc taaagcgagc aaaccgctac ttcccaatta tagagccaat actcaaaaaa
     1021 aacaatatac ccgacgattt taaatacctt gctgttgcag agagtggcct tacccaggca
     1081 gtatcacctt ccaaagcagt tggattctgg caaatacttg agggtacagc taaagaatac
     1141 gggcttgaga ttaataaaga ggtggatgag cgataccata ttgaaaagtc aactgaggta
     1201 gcctgccagt actttaagaa agcttatgag aagtatggta catggaccat ggcagcagca
     1261 tcgtacaact ttggaatgaa cggtattaca aagcaaattg accggcagga tagtaattcg
     1321 tattacgata tggttttagg cgacgaaacc ggcaggtatg tgtaccgtat attagcgctt
     1381 aaggtgattt ttgagaaccc caagcagtac ggtttctttc tggatagtac tgacctttat
     1441 ccaccacttg agtacactga ggttaaagtt gacagcacca taaccagcat tgctagtttt
     1501 gcaaggcagt acggcgttaa ctataaactc atcaaactct ttaatccctg gcttagggaa
     1561 aattacttgc caggcaagca aggcaaatcg taccttataa agataccacg taaaggtttc
     1621 cgtgaggagg catataaata ggttttagca tgtttcagga tattgataaa tcggccttaa
     1681 ccgatgaggg cgaaattgtt gttgagggtg cacgcgtgca taacctgcgg aatgttgatg
     1741 ttacaattcc acggcaaaag ctgacggtaa ttaccggcct gagcggcagc ggtaaatcat
     1801 cattagcatt tgataccata tatgctgagg gtcagcggcg ctacatggaa accatgtcgg
     1861 cctatgcccg gcaattcctg ggtgctcttg agcgacctga tgttgataag atttcagggc
     1921 ttagccctgt gatttccatt gagcagaaaa ccaccagcca aaatccccgc tccactgtgg
     1981 ggacaattac agaaatctac gatttcctcc gtctacttta cgccagagcg tcaacagcct
     2041 actcatacca aaccggggag gaaatggttc gttataccga tgagcaaatc attaacctga
     2101 taaacgaacg gtttactggt aaacgaaccg ccatactggc gcctgtggtt cgtggacgta
     2161 aggggcacta caaggaactt tttgaccagc taaggcgcaa gggttacctc aacgcccgta
     2221 tcgatgggga aataacggag ctacgaccgg ccatgaagct cgaccgttac aaaacccact
     2281 acattgaact tcttgttgac aaattccgtg taggcgaggg cgatttaaaa aggttaagcc
     2341 agtcggtggc tacagcaatg aacattggca agggaatcat catggtgctt gatatggata
     2401 acggtgaata ccggtacttt agccgcaacc tgatgtgtcc tacaactggc atttcgtacg
     2461 atgagcctga gccatacaca tttagcttta actctcccaa aggggcatgt acacactgta
     2521 atgggttggg ttacataaac caagccgata ttgaacgaat tatccccgac ccacagctga
     2581 gcattaaacg aggtggtatt caacccttgg gaccatataa aaactcactg atattctggc
     2641 aactggaagc aattgcctca aagtacgatt tttccctttc tgaccccatt ggacaaatcc
     2701 ctgatgaggc gctaaacgtg attctttacg gttccgaaga acagtttaag ctaaaacatc
     2761 caagcattgg ggtaaacagc tcatacttcc tgagctttga aggggtaatc agctacatcc
     2821 aaaaccatca aaccaacgat gagctgaatg gcaagggcga ccggtgggca aaccagttca
     2881 tcaaacaggt aacctgccca acctgcaagg gaacaaggtt gaaagaagaa tcgctatggt
     2941 tcaaggttga tggtaaaagc atagccgatg tttcagccat ggacattgaa acactctggc
     3001 attggctaca gggtcttgag aatcgtcttg gcagcaggca aaaggcaata gccaccgaaa
     3061 tacttaaaga gatcagggag cgcgtacgct ttttgctcga cgtaggattg ggttaccttt
     3121 cgttaaaccg cggttcttca accctatcgg gtggcgagag ccaacgcatc aggctggcta
     3181 cccaaattgg ttcaaaactg gttaatgtgc tttacatact cgatgagcca agtattgggc
     3241 ttcatcagcg cgataacatc aagctcataa actcgcttaa gcagctgcgc gatgcaggca
     3301 actccgtgat agtggttgaa cacgatgagg agatgatacg ctcagccgat tacgtagtag
     3361 atataggccc acgcgccggc aggcatggcg ggaaaatagt tgcaacaggc actccctcgg
     3421 aaatactgat gtccaactcg cttaccgctc agtacctcaa tggtcaactt aaaattccta
     3481 ttcctaaaga gcggcgcaag ggtaacggaa aattcctaac cgtaaaggga gcacgaggca
     3541 ataacctgaa aaatatcacc gtgaaatttc ctttaggtac atttatctgt gtaacgggcg
     3601 tatcgggtag cggtaaatca accttaataa atgaaacgct ataccctgcc ctcagccgcc
     3661 atttgtaccg ttcatatgct caaccgttgg aacacgatgc gatagaagga attgaaaaca
     3721 ttgacaaagt gattgatgtt gaccaatcgc ccattggacg cacgccccgt tcgaacccag
     3781 caacatatac taacgtattt acggatattc gcaagctctt tgagttaacc cccgatgcaa
     3841 aaataagggg atacaaggct ggacgcttct cattcaacgt taagggcgga cgatgtgaaa
     3901 cctgtaaggg cggtggcgtt caggtaatag aaatgaattt cctccccgac gtttacgtaa
     3961 agtgtcccga ctgttttgga cgtcggtata accgcgaaac gcttgaggta aagtacaaag
     4021 gtaaaaatat cagcgaggtg ctggacatga ccattaacca ggccgtggaa ttctttgaca
     4081 gcataccgca aatccatcag aagttaaaaa cgctccagga cgttggactg ggctatataa
     4141 cattggggca acaatccact accctatcag ggggtgaagc ccagcgtgtg aagctggcca
     4201 cagaattagc caaacgcgat accgggaaaa ccctctacat tctggacgag cccactacag
     4261 gcttacactt tgacgatgta cgcattctgc ttgaggtgct caacaaactt gttgaccgtg
     4321 gtaacacggt tatagtaatt gagcataatt tggatgtaat taaatcggcc gactacattg
     4381 ttgaccttgg ccccgaggga ggagcagcgg gagggcaagt ggtttgctct ggcacccccg
     4441 aagatattgc aaaatcgggt tgtggttaca catcggaatt tcttaccaaa tacttattca
     4501 gggagtagcc attttggaac ttttaccgac ccaagttcgt taaataaaaa gtaaatgcta
     4561 gcggtatgga tacactaaaa attgagaaac gaatcgactc acccatggtg ctggccgatg
     4621 gtgaatcggg atactttgag gtgaatggca aatcgttgcc cgaagatgcc attgaattct
     4681 ataaacccct tgaaaaatac gcccaggagt acgtaaaatc accaaagaaa aaaacaacca
     4741 ttaacctaaa attggagtac ctcaacacct cgtcgtcaaa aaaactactc gatatcatag
     4801 gttactttga agcattgcca tcgcaggggt acgaggtggt acttaactgg tatcaccgcg
     4861 atgaggacca ggacatgatt gatgagggta ttgagtttgc ccacatgact agccttaaag
     4921 ttaactttat agtggagcaa tagaactaag tatgtttagg tttgttcaat actatgtttt
     4981 attgattttg tttgcatttg cacattttac tgcgcaatcg caaacaaccc cgctaggcag
     5041 cagtaaggaa ctccaaaata gcttacagca agccaaggaa cttgaaagta atggcgacct
     5101 aaacggtgca ctttcaattt acaataaaac cgcaaccgct tactgggtta acggaaagct
     5161 taccgaggct cgcgacattt tcctaaaagc agcatcggtt gctgaaaaac ttggaaacca
     5221 aaatgcactc aaggtaattt acaccaacct tgggatggtg tttgttgact tggaacagta
     5281 tagcgaggcc attgcgcagt tcgaaaaaag tttagccata aaccgctcac agaaaaataa
     5341 agcggaaaca acagcaaccc ttattaacat agcaaatgct tacaaagaat tggacaagta
     5401 ccaaaaagcc cttgattacg ctgaacaggc caataccatt gcacttgaga ttaacgatgc
     5461 aaagcttctg aggaacacat actcactact ggctgaaata cacgaggcac ttggaaattc
     5521 tgaaaaatcg tcggagtact tctccctcta tacagccttt tccagaaaaa ttcaacgcga
     5581 ggaacagctc aaaaaggaag cagaggcaca gaaaattgtt gaggaagcaa agggagaact
     5641 ccaggtaata aaaagtgaaa aagagctcac agaaaaggaa ctcagcgaaa agcagcgagt
     5701 acttgaaaca gtaaaggata gtctcgataa aattgaacag ctaacgcaag agcaaaagtt
     5761 acagatagac ctgctaaata aggagaatgc actgcgcgaa gaaaaaatca aaaatcagat
     5821 tctggttcgc aacatcttta ttgtaatcat agcggctacc ataggcatgc ttggcctaat
     5881 aacccattac taccttcaaa agaaacgctc caacgaggaa cttgcacgcc aaaaagccat
     5941 ggttgaggcc aaaagccagg aactgatgca agccctccaa cagattgaga aacaaaaccg
     6001 cgatataacc agcagcatta actatgcaca gcgaatccag caagccttat tacccacata
     6061 cgaccaattg gtcagctaca tacccaatgc attcatcatg ttccgtccgc gggagattgt
     6121 aagtggcgat ttttactggt ttgctgccta tggcggccaa aatatagccg ccagtaagca
     6181 aaaccgacac atgatagctt tgcccaatat ccccaagaac gattttggtt ttctgattgc
     6241 agcagttgac tgcacaggtc acggtgtgcc cggagcattc atgtccatga ttgggctaaa
     6301 cctactcgat gtaattgtac gcagcggatt aacccaaccc aacctaatac taaatgagct
     6361 gcacaagcag gtacgttacc tgcttaaaca ggaaaataac gatagccgcg atggcatgga
     6421 catggccctt atacgaatca aaggcgatgg ccgaacaata gagtttgccg gagccaaaaa
     6481 tcccattgtt tacataacca acaaccagtg tttcaccatt aaaggcgacc ctgtacccat
     6541 tggcggttta caaaaggaag aaaaacgaga gtttacccta cataccatta ctatcgattc
     6601 acctaccagt atttacctat tttccgatgg atatatcgac cagtttggcg gacccgagaa
     6661 ccttaagttt acatccaaac gattcaggga gatgctattt gagaaccata atttacctat
     6721 ggtggctcaa caagaaaaac ttgaaacagc tcttgatgaa tggattggct ccgaaaataa
     6781 ccaaattgac gatgttcttg tcattgggct tcacctaaca ggtaaacctt ttaaaattga
     6841 tagccaaact taactgcaaa ttgataaaaa cttttatttt tgtgtataca tcacattaaa
     6901 ccatgagtaa gcaatatttt ggggttttag tttttttgtt tctttgttca tcggtgttgg
     6961 ctcaagaaaa tgccaacaat gtttatgtga acgacaatgg cgatgttttt gtaaaagccg
     7021 atgcgcctat attctttttc atcagccccg agaaccagcc ctcccagaaa gtgctcattc
     7081 ccagtaccga caaggccgct aacccgatgt attttgatgg cgacggtaaa cactacctgg
     7141 tgtatgaggg gaagggtgaa aaggtacgct tcctaatttg ggccgatgga gtgggtccta
     7201 aatcaagctt aagggttgaa aaaggacttc tctttaacca caataacagg gtttatgtgg
     7261 aggacagggc atcatttact cccagtgcaa tcgattcaaa atcgggtgta aagcaatcat
     7321 ttattgccgt tgataaccaa ccgtttg
//
LOCUS       DAOOWJ010000121         7296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_10222_length_7296_cov_7.328270, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000121 DAOOWJ010000000
VERSION     DAOOWJ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10222_length_7296_cov_7.328270"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="nfo"
                     /locus_tag="PLW22_10180"
     CDS             complement(<1..677)
                     /gene="nfo"
                     /locus_tag="PLW22_10180"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOU75111.1"
                     /translation="MKFIGAHVSAAGGVENAPVNAHSIGATAFALFTKNQRQWEAPPL
                     TQKSIDTFKANCEKYGYTPSQILPHDSYLINLGNPDRDALEKSRNAFLDEMVRCQQLG
                     LDRLNFHPGSHLNKISEEESLLLIADSINMALERTQGVTAVIENTSGQGSNLGYTFDQ
                     IRYIIDHVNDQSRVGVCIDTCHAFTAGYDISTPEAFAKTFSLFDRVIGFKFLKGIHLN
                     DTKKGLG"
     gene            780..1142
                     /locus_tag="PLW22_10185"
     CDS             780..1142
                     /locus_tag="PLW22_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75112.1"
                     /translation="MDSLTGKWTFTEEFECGTDKGFAFLEQKGDLIVGYLEYEECIED
                     EEPFMVRQIVEGNVQGHTIVLKGLEVMHPDGTPILNYNLDVLEGTYTHEKKIVGHSYD
                     SEDVCGVFVMTRVVGDEL"
     gene            1267..1713
                     /locus_tag="PLW22_10190"
     CDS             1267..1713
                     /locus_tag="PLW22_10190"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOU75113.1"
                     /translation="MPKIRLTKEFRFEMAHALEGYDGLCRHMHGHSYILAVTVIGEPI
                     DKQGHPKLGMVMDFGDLKRIVNPLIVDRFDHAVAVMANTPTHTKLREAGFNRIEALPF
                     QPTCENMIHYFAEIIKANLPVNVTLHHLKLNETATSYAEWYATDNL"
     gene            1729..4500
                     /gene="polA"
                     /locus_tag="PLW22_10195"
     CDS             1729..4500
                     /gene="polA"
                     /locus_tag="PLW22_10195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOU75114.1"
                     /translation="MKKRLLLLDAYALIYRAYYAFINRPMRNSKGMNTSAIYGFIRAT
                     LDAIKKFNPTHVAVAFDVSGKTFRNDIYPDYKAQRDETPEDIRASVPVIKNLLNALNI
                     PIIEKEGFEADDVIGTLATKANPKEFEVIMMTPDKDYGQLLSENIIIAKPGRSGNEME
                     IVTAEQFCKDYDIQDPKQFIDILALWGDASDNIKGVNKVGEKTAAKLISQFGSIDNLL
                     QNLDKLSPAQRENFEAALNIIPLNRKLVSIVTNIDLNVDIENDLAVKPPKTNELVPIL
                     EELEFRSMLKEFTATPKVEKPQYVQGSLFDMPATETPKPAINLKTINDFEVDYRIAQT
                     TEERRILIKELENSSEFAFDTETTGLDPISVELVGLSFAISPRKAWWVPVPSDQNQAK
                     EVVKEFANVFGTETIAKVGQNIKFDMQVLLSYGIDIKGHLYDTMLAHYLLEPDSRHNL
                     DYLSEIYLSYKPISIEELIGKKGSKQGSMRNVEATLITRYACEDADLALQLKHILFSK
                     LEEQGLTNLYFALEAPLVEVLTYMERNGVSLDTKSLKDYGEILSAQLVELESEIRQMA
                     GVPDLNISSPKQLGEVLFEKLKISSDAKLTKTRQYSTNEEELQKYIDAHPIVPKILEF
                     RGIKKLLSTYIETLPTLVNPKTGKIHTSFNQAVASTGRLSSNNPNLQNIPIRDENGRE
                     IRKAFIPSPGDYLLLSADYSQIELRLMAHMSTDPAMLEAFEKGQDIHAATAAKIFRIP
                     LNEVTADQRRKAKTANFGMIYGISAFGLSQRLGISRTEAKELIDGYFTTYKGVKEYME
                     KCVSQARERGWVETIFGRKRYLPDINSNNQVVRGLAERNAINAPIQGSAADIIKMAMI
                     RIHDEFKSGKLKSKMIIQVHDELVFDVYRPELDEVKAIVKKCMEGAAALSVPLEVEIG
                     TGSNWLEAH"
     gene            complement(4497..5525)
                     /gene="dusB"
                     /locus_tag="PLW22_10200"
     CDS             complement(4497..5525)
                     /gene="dusB"
                     /locus_tag="PLW22_10200"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOU75115.1"
                     /translation="MKIGNIDLGDKPVLLAPMEDVTDPSFRYMCKHFGADMMYTEFIN
                     ADGLIRDAGSSVAKLTIYDYERPIGIQLYGHIIESMVEAARVATEANPDVIDINFGCP
                     VKKIANRGAGSGMMRDVPKMVEMTRRIVEATHLPVTVKTRLGWDDQSKNIVEIAERLQ
                     DVGIKAITIHGRTRAQLYTGKADWTLIGEVKSNPRMHIPVIGNGDIDSPIRAKEMFTR
                     YGVDGIMIGRATYGRPWIFAEVKHYLKTGELMKPLSTTEKVEIARLHFEHSLQVKGAK
                     TGMLEMRRHFGSYFKGLPHFKDIRLKLLTTLNPDEVYSLLNLIAERYGDYVVDESINP
                     SPWADKQD"
     gene            complement(5614..5874)
                     /locus_tag="PLW22_10205"
     CDS             complement(5614..5874)
                     /locus_tag="PLW22_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75116.1"
                     /translation="MEKRKADRMAGIIKIPVVCIICSSFRAFKLVKLSPKTTCTPDAP
                     NIKPKSNIPINEKNAELIKMIAPKITIFPINDFDSKDPYSIN"
     gene            5906..6538
                     /locus_tag="PLW22_10210"
     CDS             5906..6538
                     /locus_tag="PLW22_10210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU75117.1"
                     /translation="MKFQTILKTFIIVLISLPAINLLASINQLIPMSDWMVEMERKAE
                     AITKAFLLTDSIWAYLVNILMIAILPAVGEELIFRGILQRKLVEFTRSRWIGILLAAA
                     LFSAIHMQFQGFIPRFALGVAFGYLLEATGSIWVPIAAHFFNNAIAATGYMLMGMGAA
                     DKRIENIGGIYDLWILGLVSLVATSLLIVSIKREFERESELKQTGDSVIE"
     gene            complement(6506..>7296)
                     /locus_tag="PLW22_10215"
     CDS             complement(6506..>7296)
                     /locus_tag="PLW22_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75118.1"
                     /translation="LYSQVISLYRSEQNKIDSAMPYRRLPNTDSARIRALKAAYKMGK
                     NTHPQELAFSQATFSKVELFINSYEKAIAHYKAAYDTQVERSKDYQQLVKKARLYLSH
                     FIQVVNMAIARGELQPAVRELYGMDIDESKLPSLNTEKDLIEWGKKIIEGEMARTRKG
                     MTPITNPTIAVVKVRYDQFLEAYNYQKVLQHNTQRMLNELNELRKKADEIILTIWNEV
                     EEHYKDLSDEERREKATAYGLVYVYRKNEIKGLSLLDNAIPRLF"
BASE COUNT         2010 a         1585 c         1676 g         2025 t
ORIGIN      
        1 ctgcctagcc cttttttggt gtcgttaagg tgaatgccct taaggaattt aaaaccaatc
       61 actctatcga aaaggctaaa ggttttggca aaagcttcgg gtgtcgatat atcgtacccg
      121 gccgtaaagg catggcatgt gtcaatgcaa accccaacac gcgattggtc gttaacgtgg
      181 tcgataatat accttatttg atcaaaggtg tagccaaggt tacttccctg ccccgacgtg
      241 ttttcaatta ccgccgttac tccctgtgtg cgctccaaag ccatattaat tgaatcggca
      301 atgagcaaaa ggctctcctc ttcagaaatt ttattcaaat ggctgcccgg atgaaagtta
      361 agcctatcga ggccaagctg ctggcaacgc accatttcat ctagaaatgc attgcgcgat
      421 ttttcaagcg catccctatc ggggttacca aggttaataa ggtaactatc gtgtggtaga
      481 atttgtgagg gtgtgtagcc atacttctca cagtttgctt taaaagtatc aatgcttttc
      541 tgggtgagtg gcggggcttc ccattggcgt tggtttttgg taaaaagtgc aaacgctgtg
      601 gctcctatac tatgggcatt tacgggggcg ttctcaaccc cacctgccgc gcttacatgg
      661 gctccaatga acttcatgac agtaaagttg aaagggttca ttaatttgcg taaatttact
      721 caataaacaa agactttgag ttagcgttca ctatctttgt aaaaattgaa tttttataca
      781 tggatagctt aacagggaaa tggacattta ctgaggagtt tgagtgcggt accgataagg
      841 gttttgcctt tctggagcaa aaaggtgatc ttattgttgg atacctggag tacgaagagt
      901 gtattgagga tgaagaacca ttcatggtaa ggcaaattgt agagggtaac gttcaaggtc
      961 acactattgt tctaaaaggc ctggaagtga tgcaccccga tgggactccc attctgaatt
     1021 acaatctcga tgtgcttgag ggtacttaca cccatgaaaa aaagattgtg ggtcacagct
     1081 acgattccga ggatgtttgt ggggtatttg tgatgacaag agtagttggc gacgaattgt
     1141 aaacgttggg aaatggaata tttttaacat ttaacctaaa accacataaa ttgcatcaag
     1201 tccttgaacc ttacccttta acttttaact tttaactttt aacttttaac ttgtaaacct
     1261 acccatatgc caaaaatccg tttaaccaaa gagtttcggt ttgagatggc ccatgccctt
     1321 gagggctacg acggcctatg ccgacacatg cacggtcatt catacatact tgcggttacc
     1381 gttattggtg agcctatcga taaacaaggg cacccaaagc tgggtatggt gatggatttt
     1441 ggcgacctaa aacgcatagt taacccccta attgttgata ggttcgacca tgctgtggcc
     1501 gtaatggcca ataccccaac gcatacaaag cttagggaag ccggtttcaa tcgtattgag
     1561 gccctgccat tccaacccac ctgtgagaat atgattcact actttgctga aattataaag
     1621 gctaatcttc cggtcaatgt aacactacat cacctaaaac ttaacgagac tgccacatcg
     1681 tatgccgaat ggtatgcaac cgacaacctg taactgttta ccaaaaccat gaaaaaacgc
     1741 ctgctattgc tcgatgctta tgccctaatt tacagggcat actatgcctt tatcaatcgc
     1801 ccaatgcgta acagcaaggg aatgaataca tcggctattt acggttttat cagggccaca
     1861 ctcgatgcta tcaaaaagtt taaccctacc catgtggcgg tagcattcga tgtatcgggc
     1921 aaaaccttca ggaacgacat ttaccccgat tacaaagccc agcgcgatga aacgcctgag
     1981 gatattaggg catcggttcc tgttattaaa aaccttttaa acgctcttaa cattccaatt
     2041 attgaaaagg agggatttga ggccgacgat gttattggca cacttgccac aaaggcaaac
     2101 cccaaggagt ttgaagtgat aatgatgacc cccgataagg actacggtca acttttaagc
     2161 gagaacatta taattgccaa gccgggtagg agtggaaacg agatggagat tgttacagca
     2221 gagcaattct gtaaggatta cgatatccaa gatccaaagc aatttatcga tattcttgcc
     2281 ctttggggcg atgcctccga taatatcaag ggtgttaaca aggtgggtga gaaaaccgct
     2341 gctaagttaa tttcccagtt tgggagcatc gataatctac ttcaaaatct cgataagctt
     2401 agcccagcac aaagggagaa ctttgaggct gcacttaaca ttattccgct taaccgtaag
     2461 ctggtaagta ttgttaccaa tatcgatctc aacgttgata ttgaaaacga ccttgccgtg
     2521 aaaccgccta aaaccaatga gctagtacca atactggagg agctggagtt taggtcgatg
     2581 ctcaaggagt ttactgcaac gccaaaagtg gaaaagccac agtacgtcca gggctcgctg
     2641 ttcgatatgc ctgcaaccga aacaccaaag ccagccatta acctcaaaac cattaacgat
     2701 tttgaggttg attaccgcat tgcgcaaacc acagaggaac gtcgaattct aattaaagaa
     2761 ctggaaaatt ccagtgagtt tgctttcgat accgagacaa ctgggcttga cccaatatcg
     2821 gttgagctgg ttggcttatc ctttgccatt tcgccgcgta aggcttggtg ggttccagta
     2881 ccatcggatc aaaaccaggc aaaagaggta gttaaggagt ttgccaatgt atttggtacc
     2941 gaaaccattg ccaaggtagg acagaacatt aagttcgata tgcaggtgct gttaagctat
     3001 ggaattgaca ttaaggggca cctttacgat accatgcttg cccattacct gttagaaccc
     3061 gactcgcgcc ataatctcga ttacctttcc gaaatctacc tgagctacaa gcctattagc
     3121 attgaggaac ttatcgggaa gaagggttcc aagcagggta gcatgcgcaa tgttgaggct
     3181 acgctaatta cccgatatgc ctgcgaggat gccgatttag ctttgcagct caagcatata
     3241 ctgttctcta agctcgagga gcagggctta accaatctgt attttgccct tgaagcaccg
     3301 ctagttgagg tgctaactta catggagcgc aatggtgtat cgctcgacac caaaagctta
     3361 aaggattacg gggagattct ctcagcacag ctggttgagc tcgaaagtga gatacgccaa
     3421 atggccggag tgcccgattt gaatatctca tcgcccaaac agcttggcga ggttctgttt
     3481 gaaaagctta agatatcgtc cgatgccaag cttaccaaaa ccaggcagta ctcaaccaac
     3541 gaggaggaac tccagaagta tatcgatgcc catcctattg ttcccaaaat attggagttt
     3601 agaggcataa agaaattgct ttcaacctat atcgaaaccc tacccacgct ggtaaatcca
     3661 aaaacaggga aaatacacac ttcgttcaat caggcggtag cttcaaccgg aaggttaagc
     3721 tctaacaatc ctaacctgca aaatattccc atacgcgatg agaacggacg tgagattcgg
     3781 aaggctttta ttccatcgcc aggcgattac ctgctactat cagccgacta ctcccagata
     3841 gagctgcgcc ttatggcgca tatgagtacc gaccccgcca tgctagaggc ttttgaaaag
     3901 gggcaggata ttcacgctgc aaccgctgca aaaatattca ggatacccct gaatgaggtt
     3961 actgctgacc aacgccgaaa ggccaagaca gccaactttg gaatgatata cggcatatca
     4021 gcgtttggct tatcgcagcg gttaggtatt tcaagaaccg aagcaaagga gctgattgat
     4081 ggctacttca caacctacaa gggagtgaaa gagtacatgg aaaaatgcgt ttcgcaggct
     4141 cgtgagcggg gatgggttga aactattttt ggacgtaagc gctacctgcc cgatatcaac
     4201 tcaaacaacc aggtggttcg tgggcttgct gagcggaacg caattaatgc tcccattcag
     4261 ggctcggccg ccgatatcat caaaatggcg atgattagaa tacacgacga gttcaagagt
     4321 ggcaagctaa agtccaaaat gataattcag gttcacgacg agttggtatt cgatgtttac
     4381 cgcccagagc tagatgaggt aaaagcaata gtaaagaaat gcatggaggg tgcagccgca
     4441 cttagcgttc cgctggaggt ggagattggt actggcagca actggcttga ggcgcactag
     4501 tcttgcttgt cggcccaggg cgagggatta atggactcgt caaccacata gtcgccataa
     4561 cgttcagcta ttaagtttag gagtgagtaa acctcatcgg ggttaagggt ggttagtagt
     4621 tttagcctga tatccttgaa gtgtggtaat cctttaaagt atgagccaaa atgccttcgc
     4681 atttcgagca ttccggtttt ggctccttta acctgcagcg aatgctcaaa gtgcaagcgg
     4741 gcaatttcaa ccttttcagt ggtagagagg ggtttcataa gctcaccggt tttaaggtaa
     4801 tgcttcacct cagcaaaaat ccatggacga ccataggtgg cgcgcccaat cataattcca
     4861 tcaaccccgt aacgggtaaa catctccttg gccctaattg gactatcaat atcgccgttg
     4921 cctataacgg gaatgtgcat ccgaggatta ctcttaacct caccaatgag tgtccaatcg
     4981 gctttgccgg tataaagttg agcgcgggtg cggccatgaa tggtaatggc ctttatccca
     5041 acatcctgaa ggcgttcagc aatttcaaca atatttttcg attggtcgtc ccaacccaag
     5101 cgggttttaa ctgtaactgg caaatgggta gcctccacaa ttcggcgggt catctcaacc
     5161 attttgggaa catcgcgcat catacctgag cctgcgccgc ggttggcaat ctttttaacc
     5221 gggcatccaa agtttatatc aataacatcg gggtttgcct cggtggcaac ccgggcagcc
     5281 tcaaccatgc tctcaattat atggccgtaa agctgaatgc caatgggtcg ttcgtaatcg
     5341 taaatggtta gttttgctac actgctccca gcatcgcgga taagcccatc ggcattaata
     5401 aactcagtat acatcatatc ggcaccaaag tgcttgcaca tgtaccggaa cgatgggtcg
     5461 gttacatcct ccatgggagc cagtaacaca ggtttatcgc ctaaatcaat attccctatt
     5521 ttcatccatt tgattttgcc atgcaaaaat atcaaaagaa aactgttaaa ggctttgttg
     5581 aaagctgtta tgaattactt ttgtaaaaat tttttagttt atggaatatg ggtcttttga
     5641 atcaaaatca tttattggaa agatggtgat ttttggagct atcattttta ttagctccgc
     5701 gtttttttcg ttaattggaa tattgctttt aggtttaatg tttggtgcat cgggagtgca
     5761 ggttgtttta ggagacaact taactaattt aaatgctcta aaactgctgc aaataataca
     5821 aaccacagga atttttatta taccagccat tctatcggcc tttctttttt ccaaaaaacc
     5881 atggagttac ctatgctttg aaaaggtgaa attccaaaca atactcaaaa catttattat
     5941 agtacttatc tcacttccgg ccataaacct tttggcatcc atcaaccagc tgattcccat
     6001 gagcgactgg atggtagaga tggaaaggaa ggcagaggct attacaaagg cctttctgtt
     6061 aaccgatagt atttgggctt acctggttaa tatattgatg attgccatcc tccccgcagt
     6121 aggtgaggag ctcattttca ggggaattct tcagcggaag ttagttgagt ttacacgctc
     6181 gcgatggatt ggtattttgc ttgcggctgc gctgtttagc gctatacaca tgcagtttca
     6241 aggttttata ccccgctttg cgctgggcgt tgcgtttggt tacctgcttg aggctacagg
     6301 tagtatatgg gttcccatag ctgcgcattt ctttaacaat gccattgccg ccacaggtta
     6361 tatgcttatg ggtatgggtg cggccgataa gagaattgaa aatattggtg gtatatatga
     6421 cctttggata cttgggcttg ttagcctggt agcaacttcg ctgctaatag taagtattaa
     6481 aagggagttt gaacgggaaa gcgaactaaa acagacgggg gatagcgtta tcgagtagcg
     6541 ataacccctt tatctcgttt tttctataaa cgtaaaccaa accgtaagcg gttgcctttt
     6601 ccctacgttc ctcatccgac aggtctttgt agtgttcctc cacttcgttc cagatggtta
     6661 ggattatttc atcggctttt ttacggagtt cattcagctc attaagcatg cgctgagtat
     6721 tgtgttggag cactttttgg tagttatagg cttcaagaaa ctggtcgtag cgcaccttaa
     6781 caacagcaat agtggggttg gtaattggtg tcatcccttt acgggtgcgt gccatttcac
     6841 cctcaattat ctttttgccc cattctatga ggtctttttc agtattgagt gagggtagct
     6901 ttgactcatc aatatccata ccgtaaagtt cccgcactgc tggctgtagt tcaccccggg
     6961 caatggccat gtttactacc tgaataaagt gcgaaaggta caatctagcc ttttttacca
     7021 gttgctggta atccttgctt cgttcaacct gagtatcgta tgccgccttg tagtgtgcta
     7081 tagccttttc gtatgagttt atgaatagtt ccaccttgga aaatgtcgcc tgcgaaaagg
     7141 ctaattcctg cgggtgggta tttttgccca ttttataggc agctttgagc gcgcgaatac
     7201 gagcgctatc ggtgttgggt aatcggcggt agggcatagc actatcaatt ttgttttgct
     7261 ctgaacgata aaggcttatc acttgcgaat ataatc
//
LOCUS       DAOOWJ010000122         6962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_10904_length_6962_cov_7.665412, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000122 DAOOWJ010000000
VERSION     DAOOWJ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10904_length_6962_cov_7.665412"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..99)
                     /locus_tag="PLW22_10220"
     tRNA            complement(26..99)
                     /locus_tag="PLW22_10220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Pro,seq:tgg)
     gene            complement(116..204)
                     /locus_tag="PLW22_10225"
     tRNA            complement(116..204)
                     /locus_tag="PLW22_10225"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Ser,seq:gct)
     gene            complement(336..1316)
                     /gene="floA"
                     /locus_tag="PLW22_10230"
     CDS             complement(336..1316)
                     /gene="floA"
                     /locus_tag="PLW22_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695856.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOU75119.1"
                     /translation="MNEVGALLLIIALSVVGLWIIFYFVPLGLWFQALVSGVRISLLQ
                     LIFMRWRKVPPRVIVQAMIEGTKAGLTLNRNEMEAHFLAGGRVSKVVHALVSAQKANI
                     PLDFKMATAIDLAGRDVFEAVQMSVNPKVINTPPVTAVAKDGIQLITKARVTVRANIK
                     QLVGGAGEETVLARVGEGIVTAIGSSLSHKEVLENPDKISKTVLDKGLDAGTAFEILS
                     IDIADIDIGRNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKAQEARAKVI
                     EAEAEIPKAIAEAFRSGNLGVMDYYRIKNIEADTNMRDNIAKSGNEPKKK"
     gene            complement(1326..1784)
                     /locus_tag="PLW22_10235"
     CDS             complement(1326..1784)
                     /locus_tag="PLW22_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOU75120.1"
                     /translation="MFWIIALIILGLFLLVVEFFMIPGVTVAGIGGFLIIIYAVYSAY
                     ADHGTTAGHLTLLFTAIASIATLVLSLRARTWKRVSLETSISSKAVEELENEIQVGDE
                     GVTISRLAPMGKASIKGKTVEASTIGELINPNTPIVVVHVETNKIIVKPK"
     gene            complement(1795..2076)
                     /locus_tag="PLW22_10240"
     CDS             complement(1795..2076)
                     /locus_tag="PLW22_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOU75121.1"
                     /translation="MAVKEKVQGKGKLLPSAELEYILILHNDDVNTFDFVIDTLVEVC
                     NHDPHQAEQCALITHFKGKCDIKHGEFEMLEGMMNELHRRGLSATIASN"
     gene            complement(2112..4898)
                     /locus_tag="PLW22_10245"
     CDS             complement(2112..4898)
                     /locus_tag="PLW22_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75122.1"
                     /translation="MKWLKIIALLPFFFSSVSSWCQVDSIGAKSGGMQRAMADSLLNA
                     KSYTKAIPKYKELLVQFPRDPEIKYALGVCYLYGTRDVEMALKYLKDASTSDLNTMVY
                     FYLAEALRQSYQFNEAIDYYRRFVVSGGSPDIKLDEVEKLVTACENGSFLTRYVYSPT
                     VFDRKQASPSNFLNYYSSLLPEGSFTPVPENLRTVTDKQMGYTSVMFYPKNIKPGDYL
                     YYSSYGKSTSWGTDIFRIQLLDNGKWSKPENLGDIINSSLNEEYPFIMPDGVTLYFAS
                     KGHYGMGGYDIYKSVYNADKKQWSTPENLGFPVNSTYDDFLFLTGNNDTLACFTSNRL
                     SMPDTLNVFLIRNDINPTRRTPNSYKELLSIARLNPVESKPSASPKVPSSKVETDKPK
                     AEAVENKKPAKFNSVENDPEYSRVLAKGFAAQKLADSLKIKLEDLRGRFDYVTTAEQR
                     VRLEKQVTKVEDDMLAAQKEADLLFARASQIEQEYLTGKRKPKDESNTSFVADNPDFI
                     YQAQFASTVFRTDEINRLADAEKLTPQLIRFRNFALERRDAYNRCLSANDNNNACTAE
                     YNQLVAAMKQYSDHFKKYWDKKYQIYSDCIEVARVKSGNRDTEVRKLTTDAQNNFRAA
                     MAILNNLDSTGQVESVFEASLLRDLGLMQFDLAFAKIWRLRLMEQQLLSNVIKLERNI
                     FGQSSLNLAQEEQKPTKTVEEVEVDMPKLKSEPAIKQATPTIAIIDEIPADFGVTDKP
                     YYSDEKPIPIDEPLPSGVIYRIQIGAFSAPQKPSFFKGMVPVYGYKTGNIIRYYIGNL
                     TRYSDAEKALVTVKQKGFKDAFIVAWLNGTRITTQRAQQLEDAVQQVQQSKTDSRLYI
                     VEVGRYKTPLTDSEINTVRNLSQGKELTRKVDSNGDHIYIIGNFNSIDEANRVKENLI
                     ASGMLKAVVIDITP"
     gene            5040..5285
                     /locus_tag="PLW22_10250"
     CDS             5040..5285
                     /locus_tag="PLW22_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HOU75123.1"
                     /translation="MKVNQSIAISENGFIFNPITGDTFTLNDSGFQILTMLKSGKSQD
                     EIKDKLLSEYEVDELVLERYLQDFISDLKRNNLLLEE"
     gene            5290..6336
                     /locus_tag="PLW22_10255"
     CDS             5290..6336
                     /locus_tag="PLW22_10255"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOU75124.1"
                     /translation="MERKKITIAITGLNNIDSPGPGMPVIRGIKESTDFDTRIVGLAY
                     DHLEPGIYMKEWVDKTYMIPYPSKGKEALFERIKEINAKENIDLIIPNFDAELFSFIK
                     LEKQINQLGIKTFLPTLEQFEERHKSSLPEYGKKYGVNVPKSEEAYSMNDLANVLRSY
                     TYPVMVKGKYYDAYKAYSQEQAQAYFLKVSAKWGYPVIVQEFVEGTEVNVIALGDGNG
                     QVISAVAMRKQYITDKGKAWGGVTIADDSMLELTHKIISQTKWRGGMELELIRTADNK
                     LYILEINPRIPAWVYLAVGAGQNLPEALVKLAMGIDVKPYTSYEIGKMFIRYSWDMIV
                     DIDMFSKLVTSGEL"
     gene            6357..>6962
                     /locus_tag="PLW22_10260"
     CDS             6357..>6962
                     /locus_tag="PLW22_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75125.1"
                     /translation="MEKKNFERPTITKLTPGMPSKHGVGIAVAPVQFIDGIKVDELIE
                     KYGSPLYIFSESVIRKNVNDLKRAFSVRYPKVQIAWSYKTNYLDAICRVFHQEGSWAE
                     VVSGFEYEKARLNGIPGEHIIFNGPGKTEAELLRAAKEGAYIHIDHFDELFLLAKVCQ
                     ANSVNARVAIRVNMDVGIYPKWDRFGFNYENGEAWDAINRIM"
BASE COUNT         1944 a         1439 c         1569 g         2010 t
ORIGIN      
        1 aagcggtttg cttccttgtc accagtcggg gtggtgcgac tcgaacgcac gaccccctgc
       61 tcccaaagca ggtgcgctaa ccaactgcgc cacaccccga acgttaaact ttcagcggag
      121 agggggggat tcgaaccccc ggtacccctt gcggagtacg acggtttagc aaaccgttgg
      181 tttaagccac tcacccacct ctccgggctt acttaatcag ccttgtttcc gctaaaagca
      241 acgcaaaagt ataaaatctt attttatgtg caaaataatt acaaaaaaaa gagggattgc
      301 tccctctctt ctattgatta tcagtttact tatagttatt tctttttagg ctcgttgccc
      361 gatttggcaa tgttatcgcg catgttggtg tcggcctcaa tattcttaat acgatagtag
      421 tccatcacac caaggttacc gctacggaat gcctcggcaa tagctttagg aatctcagct
      481 tcagcttcaa ttacctttgc acgggcttcc tgggctttgg ctttcatttc ctgctcaagg
      541 gccactgcca ttgccctgcg ctcctcggcc ttagcctgtg caatattttt atcggcgtta
      601 gcttgatcca tttgcagtac ggctccaatg ttacggccta tatcaatatc ggcaatatca
      661 atcgaaagga tttcgaaagc ggttcctgca tctaacccct tatcgagaac ggttttggat
      721 attttatcgg ggttttcaag cacttccttg tggctaagcg atgaacctat ggcggtaaca
      781 ataccctcgc caacacgggc caaaacggtt tcctcaccag ctccaccaac cagctgcttg
      841 atgtttgccc tcacggtaac acgggctttg gtaatgagct gaataccatc ctttgctacg
      901 gctgttactg ggggtgtatt aatcactttt gggtttaccg acatctgaac agcttcaaat
      961 acatcgcggc cggcaaggtc aatggcagtt gccattttaa aatcgagggg gatattggcc
     1021 ttttgtgccg ataccaaggc atgaacaact ttggaaaccc gaccaccagc caggaagtga
     1081 gcctccatct cgttacggtt aagggtaagg cctgcctttg tcccctcaat catggcctgt
     1141 actattaccc gtggcggaac cttacgccag cgcataaaaa taagttgcag gagcgatatc
     1201 ctaactcccg ataccagcgc ttggaaccaa agtccaagag gtacaaagta aaagattatc
     1261 cataagccta caacgctcag ggcaataatc agaagtagtg caccaacttc attcatagtt
     1321 tagagttatt taggtttgac aataatttta ttagtttcaa catgtactac aacaatgggt
     1381 gtgtttgggt taataagttc acctatggtt gaggcctcga cggttttacc cttaatggag
     1441 gctttgccca ttggtgcaag acggctgatt gtaacccctt catcgcccac ctgtatttcg
     1501 ttctctagct cttcaacagc ttttgaactg atacttgttt cgagcgaaac acgtttccag
     1561 gttcttgccc ttaatgataa aacaagagtt gcaattgatg caattgctgt aaaaaggagc
     1621 gttaaatgtc ctgctgtggt tccatggtcg gcgtaggcag agtaaacagc gtatataatg
     1681 attaaaaaac ctccaatgcc agcaactgta acaccgggaa tcataaagaa ttctaccacc
     1741 agcaaaaaca gacctaaaat gataagtgca ataatccaga acatagttta gctattagtt
     1801 tgatgcaatg gtagcgctaa gccctcggcg atgcaactcg ttcatcatac cctcaagcat
     1861 ttcaaattcg ccatgcttaa tatcgcactt gcctttgaaa tgggtaataa gggcgcattg
     1921 ctctgcctga tgaggatcgt ggttgcaaac ctcaaccaag gtatcgatta caaaatcaaa
     1981 ggtattaaca tcgtcgttgt gaaggattaa aatgtactca agttctgccg aaggcagaag
     2041 ttttccttta ccttgaacct tttccttaac tgccatatca cgtaaacatt tattaggcta
     2101 atatagctat tttatggtgt aatatcaata acaaccgcct tgagcattcc gcttgcaatc
     2161 agattttcct ttacgcggtt ggcctcatcg atactgttaa aatttccaat gatgtaaatg
     2221 tgatcgccat tggaatcaac cttacgggtt agctcctttc cctgcgagag gtttcgaaca
     2281 gtattgattt ccgagtcggt taaaggggtt ttgtatcgtc ctacttctac tatgtacaag
     2341 cggctgtcag tctttgactg ttgaacctgc tgtactgcgt cctcaagttg ctgagcgcgt
     2401 tgggttgtaa tacgtgtccc gttgagccag gccacgataa aagcatcttt gaaacccttt
     2461 tgcttaactg taacaagcgc cttttccgca tcggagtagc gggtaaggtt acctatgtag
     2521 tagcgaatga tgtttccggt tttatatccg taaacaggaa ccataccctt aaagaatgaa
     2581 ggtttttgtg gggcgctgaa agcgccaatc tgaatacgat agattacccc actgggaagt
     2641 ggttcatcaa tgggtattgg cttttcgtcg gaatagtagg gtttatcggt aacgccaaag
     2701 tcagcgggta tttcgtcaat tatggcaatg gttggtgtgg cctgcttaat ggccggttca
     2761 ctttttagct taggcatgtc tacttcaacc tcttccacgg tcttggtagg tttttgttcc
     2821 tcctgagcta ggtttaggct tgattggcca aaaatatttc tttcaagctt aatgacattg
     2881 cttagcagct gttgttccat aaggcgtaat cgccaaattt ttgcaaaggc aaggtcaaac
     2941 tgcattaacc ctaagtccct taaaagtgaa gcctcaaaaa cgctttcaac ctggccggtt
     3001 gaatcgaggt tgttaagaat ggccattgcg gccctaaagt tgttttgggc atcggtggtt
     3061 agctttcgaa cttcggtatc gcggttacca ctttttacac gggctacttc aatgcaatcg
     3121 gagtaaattt gatatttttt atcccaatac tttttaaaat ggtcggagta ttgtttcatg
     3181 gctgcaacca gctgattata ttcagctgtg caagcattat tgttgtcatt agctgagagg
     3241 catctattgt aggcatccct cctctcaagg gcaaaatttc tgaaacggat aagctgtggt
     3301 gttaactttt cggcgtcggc aagtctattg atttcgtcgg tgcggaaaac ggttgaggca
     3361 aactgtgcct gatagataaa atcggggtta tcggcaacaa acgaggtgtt gctttcgtcc
     3421 tttggtttgc gtttaccggt aaggtattcc tgctcaattt ggctggcacg ggcaaaaagc
     3481 aaatcggcct ccttttgggc agcaagcata tcatcctcaa ccttggttac ctgcttttcg
     3541 agcctaaccc gttgctcggc tgtagttaca taatcgaaac ggcctcgcaa gtcctctagc
     3601 ttaattttaa gcgaatcggc aagtttttgt gcagcaaagc ctttggctaa aacacgacta
     3661 tactcagggt cgttttctac ggagttgaac tttgctggtt ttttattctc aacggcttca
     3721 gccttaggtt tatcggtttc tacttttgaa ctgggtactt taggtgaggc actaggtttt
     3781 gactcaacag gattaagcct tgctatggag agcaattcct tgtagctgtt tggagtgcgt
     3841 cgggtagggt tgatgtcgtt tcggataagg aaaacattta atgtatcggg catgcttagt
     3901 cggttcgagg taaagcatgc aagtgtgtcg ttgttaccag ttaggaataa aaaatcatcg
     3961 tacgttgagt ttaccggaaa gcctaaattc tcaggggtac tccactgctt tttatcggcg
     4021 ttataaaccg atttgtaaat atcgtagcca cccattccgt aatgtccctt cgatgcaaag
     4081 taaagggtaa cgccatcggg cataataaat gggtactcct catttaacga ggaattaata
     4141 atatcgccta aattctcggg cttgctccac ttcccgttat caagtagctg aatgcggaaa
     4201 atatcggtac cccacgatgt ggacttgcca tagctggagt agtaaaggta atcgccgggt
     4261 ttaatattct tggggtaaaa cattacagag gtgtaaccca tttgcttgtc ggtgactgtg
     4321 cgaaggtttt cggggactgg agtaaaagaa ccttcgggca atagcgatga gtagtagttc
     4381 agaaagttac tggggctagc ctgttttcgg tcgaaaacag tagggctata aacgtaacgg
     4441 gtgaggaagg agccgttctc acatgcagtt acaagttttt caacttcatc gagtttaatg
     4501 tcgggtgatc ctccgcttac aacaaatcgg cggtagtagt caatggcttc gttaaactgg
     4561 tacgactgtc gtaaggcctc ggcaagataa aagtaaacca ttgtattgag atcggaagtt
     4621 gatgcatcct tcaggtattt cagcgccatc tctacatctc ttgtcccgta aaggtaacat
     4681 acacccaatg catactttat ttcaggatcg cgggggaatt ggacaagtag ttccttgtat
     4741 ttagggattg ccttagtgta gctttttgca tttagtaatg agtcagccat agctctctgc
     4801 atgcctccac tttttgctcc gatgctatct acctgacacc acgatgaaac tgatgaaaag
     4861 aaaaatggga gtaaggctat tattttcaac cacttcatat cttcaatgcg ttggaatatt
     4921 ttaagacatt caaaaatagt gtattaaagc caaattgaaa aatattaatt tttaaagtag
     4981 ggtattttta aaatgggttg taacaaaaaa tatagtaatt taacataaaa ctccttgcca
     5041 tgaaagtaaa tcaaagcata gccataagcg agaacgggtt tatattcaac ccaattacag
     5101 gagacacatt cacacttaac gattccggct ttcaaatttt aacaatgcta aaatccggaa
     5161 aatcgcaaga tgaaattaag gataagttgc tttcagagta tgaagttgac gagttagtcc
     5221 tggaaaggta tttacaggat tttattagcg atttaaagcg gaataattta ctacttgagg
     5281 agtaggacca tggagcgtaa aaaaataaca atagccatta ccggcctcaa taatattgat
     5341 agcccggggc ctggtatgcc agtaattaga ggcataaaag agtcgactga ctttgatacc
     5401 agaatagttg gtttggctta cgatcaccta gaacccggaa tctacatgaa ggaatgggtg
     5461 gataaaacct acatgattcc ttacccctca aaaggtaaag aggcattatt tgaaagaatc
     5521 aaggaaatca atgctaaaga aaacatcgat ctgattattc ccaatttcga tgctgaactt
     5581 ttctcgttta taaagcttga aaaacaaatt aaccagcttg gtattaaaac atttcttcct
     5641 accctggagc agtttgagga gcggcataaa tcaagcttgc cagagtacgg taaaaagtat
     5701 ggcgtaaatg ttccaaaaag cgaagaggcg tacagtatga atgaccttgc aaatgtttta
     5761 cgttcatata catacccagt catggtaaag ggaaaatact atgatgccta caaggcatac
     5821 tctcaggaac aggctcaagc ctattttttg aaagtgtcag ctaagtgggg gtatcctgta
     5881 attgtgcagg aatttgttga aggaaccgaa gtgaacgtaa ttgcccttgg cgatgggaat
     5941 gggcaggtga tttcagcggt tgccatgcgt aagcagtata ttacagacaa aggcaaagcc
     6001 tggggagggg taaccatcgc ggacgattca atgcttgagc taactcataa aatcatttca
     6061 caaacaaagt ggaggggtgg catggagctg gagcttattc gcacagccga taacaagcta
     6121 tatatccttg aaataaatcc tcgaattccg gcttgggttt accttgctgt tggtgccgga
     6181 caaaatttac ccgaagcatt agtaaagctt gccatgggaa tagatgttaa gccctacacg
     6241 tcttacgaaa ttgggaaaat gtttatccgt tactcatggg atatgatagt agatattgac
     6301 atgttttcaa agctggtaac cagtggagaa ttgtagaata ctaaaaggtt aaactaatgg
     6361 aaaagaaaaa ttttgaacgt ccaaccatca caaagctaac gcccggaatg ccctcaaagc
     6421 atggcgtagg cattgcagta gcacctgtgc aatttattga tggtataaaa gttgacgaac
     6481 taattgaaaa gtatggctcc ccactttata tcttttcgga atcggtaatt cgcaaaaatg
     6541 ttaatgattt aaaacgcgca tttagcgtgc gttacccaaa agtgcaaatt gcatggagct
     6601 ataaaactaa ctacctggat gccatttgca gggtttttca tcaggaagga tcatgggccg
     6661 aagtggtttc gggatttgaa tacgaaaagg ctcgactcaa tggcattccc ggtgaacata
     6721 ttatttttaa cggcccagga aaaacagagg ccgaactttt aagggcagct aaggagggtg
     6781 cttacattca cattgatcat ttcgatgagc ttttccttct tgccaaggta tgtcaagcca
     6841 atagtgttaa tgcccgggtt gctattaggg tcaacatgga tgtaggtatt tatcccaaat
     6901 gggataggtt tgggtttaac tatgaaaatg gcgaggcttg ggacgcaatt aacagaataa
     6961 tg
//
LOCUS       DAOOWJ010000123         6930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_10987_length_6930_cov_7.000873, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000123 DAOOWJ010000000
VERSION     DAOOWJ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10987_length_6930_cov_7.000873"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLW22_10265"
     CDS             complement(<1..622)
                     /locus_tag="PLW22_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU75126.1"
                     /translation="MDQINIAELYRLVKDFFEHDTTGHDWWHVQRVTQMALHIAKAEG
                     ANLALVEPAALVHDTDDWKLSGANGYPNATRMLLAVGYNNHQVDRILEIVEQVSYKGA
                     GVDTAPSSLEAMVVQDADRLDAIGAIGIARAFAFGGSKHRPLYLPNVKPQLHSTFEEY
                     KTAKGHTINHFYEKLLLLKDRMNTATAKALAEERHRFMESFLEQFYR"
     gene            complement(705..1097)
                     /locus_tag="PLW22_10270"
     CDS             complement(705..1097)
                     /locus_tag="PLW22_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75127.1"
                     /translation="MGIQYYGICKNCGRRFKIMDGGGFTFHMLRCDTCGKHKSVNFEQ
                     LGELHLRYLKGLNQPYSLATAHYDNLIRELFPGEPLPKDSYEAAIEEFVGKCKCGGQF
                     RFNASPRCPRCRSTDFEEDPNGDAFLYD"
     gene            complement(1190..2329)
                     /locus_tag="PLW22_10275"
     CDS             complement(1190..2329)
                     /locus_tag="PLW22_10275"
                     /inference="COORDINATES: protein motif:HMM:NF025642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HOU75128.1"
                     /translation="MLGKSTLSYGKVKEQHLTRWIFWNKQNGTTPEKFVVEVSNSTGT
                     VLSIPSNFSFLSSAEHLDTFCNPLGIDWLVKAFPYPILFAHALLLKPIIERRVTNPLA
                     LAQLIVSDELSEKVSPRMFYRNVLSQPFFDSWDKINVALLLLMVAQNAQTTLERIEKL
                     TMQYARNYQYFRYINLAYYCMVKADCCIAHQKLSEMLELLSKENIQSSGDVSPIRFNN
                     SYDSTRELLAGFHDYAINLKSLSNLSQKPKPLYTDSLTLPKGITLIDTPEALAAEGKK
                     MRNCVASYTRELMAKRSFFLHVSGDEKATVQVEKNSAKRCFEITQIKGVANVEVSKET
                     VNRVRKAISTFDAQRFFFLNYRKKSNKRKNIQSGPTLFDGLEGWE"
     gene            2688..3854
                     /locus_tag="PLW22_10280"
     CDS             2688..3854
                     /locus_tag="PLW22_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU75129.1"
                     /translation="MEQLNIIYHELTDKVNTRFTRYLLPKINWNARLIAITGPRGVGK
                     TTLMLQKIKIENKIDESLYVSLDNIYFSNHTLFELAGYFYRYGGRHLFIDEVHKYETW
                     SQEIKNIYDSFPDLQIVFSGSSILDIYKGFGDLSRRVVPYHLNGLSFREYLIFETGQD
                     SEAYSLDQILSQKVKIKLENPLIHFKKYLNHGYYPFYKDDDFMIRLTGTVNAVLEVDI
                     PKYLDLKVSTIDKLKHLLQIISESVPFKPNYSKIADLIGVSRNLLPDYLAYLEKAGLI
                     LELTNETKGIRRLGKVNKVYLNNPNLAYMLSGENANIGNIRETFFFNQLKLNHKVFSS
                     ETVDFKVEGNEFEIGGKSKGNKQISKLSNAYIVKDDIEYGFGNTIPLWHFGLLY"
     gene            3967..5253
                     /locus_tag="PLW22_10285"
     CDS             3967..5253
                     /locus_tag="PLW22_10285"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU75130.1"
                     /translation="MTRRIQPFAVYSDGKGNIYENPKLFAAGRSGNRVYPLYLDEMIP
                     LPYGSELFELPGRKVLGYSRKGELVESNQGIACAAFIAPAHTQLSIAAWVNTPNAPVL
                     PLYAYTAIGWYNGQFYVPAIRIDPDTRQDCENFNQKIIIKRGKELKLRFPNNRLIHHL
                     VDNCAFTYLCPAARNYVMNRWEAPLPTSPTCNARCVGCISKQDKSESPVHESQHRLTF
                     VPTVDEIVEVAVPHLETAPNPVVSFGQGCEGEPLMQWELLRDAIKAIRAKTSKGIINL
                     NTNGSRPKAVEELFRAGLNSIRVSMNSAQEKWYNLYIRPVNFTQADAVESLRIAHRFN
                     AWSSINYFVMPGITDTAEELNALKVIIESARINMIQWRNFNIDPDWLFSRIEFNGQGE
                     PLGVNQIMEQIRILYPWVYFGYFNPPLNIIEQYQFK"
     gene            5264..5494
                     /locus_tag="PLW22_10290"
     CDS             5264..5494
                     /locus_tag="PLW22_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75131.1"
                     /translation="METYICKACNLKFDANGTKTEWIDAAFGPCSKLIAKCPECGNEC
                     DEFRIPKQGSTDSYQHASHKPACDSCCGGCGI"
     gene            5514..5759
                     /locus_tag="PLW22_10295"
     CDS             5514..5759
                     /locus_tag="PLW22_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU75132.1"
                     /translation="MVEIKVLGIGCPNCHKLEAMCHEVVNELGIDAHIESVTDMNQFM
                     DYGVLLTPALVINNEVKVQGKLPLKPTLVNWIKQGDK"
     gene            complement(5797..6729)
                     /locus_tag="PLW22_10300"
     CDS             complement(5797..6729)
                     /locus_tag="PLW22_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOU75133.1"
                     /translation="MALKNQRKDLIRIGVFYDGNYFLHVSNYYNYFHQRKKRLNIAGL
                     HEFIRHMVANEEGVDPNFCQLIEAHFFRGRISAQEASQRGNQLYNDRVFDDILMSEGV
                     ITHYLPIRPRGGRREDKGIDVWLALEAFEQTLYKRFNVLVLITSDGDYVPLIRKINSL
                     GTKVMVLAWDFDYTDDQGREVTTRTSQELLREVTYPLPMHELINDGLKRKDKVVENLF
                     AVQDYSQPRQATKEIITDEEAKRARILSVNNGYGFIQYPPNNLFFHYQDVEDGEFEEL
                     IEGDMVEFVIGINEKGQEVAKRVRKVPDYDVNTW"
BASE COUNT         2047 a         1512 c         1416 g         1955 t
ORIGIN      
        1 ccctgtagaa ttgctctaaa aaactttcca taaacctatg cctctcctcg gccaaggctt
       61 tagccgtagc ggtgttcatc ctatccttca ggagtaaaag cttttcgtag aaatggttaa
      121 tggtgtgccc tttggccgtt ttatactcct caaaggttga atgtagctga ggtttcacat
      181 tgggaaggta caagggacgg tgctttgaac ccccaaaggc gaaagcccgg gcaatgccaa
      241 ttgcgccaat ggcatcgagc ctatcggcat cctgaacaac catggcctcc aggctgctag
      301 gtgcagtatc gaccccagcc cctttgtacg aaacttgctc cactatttcc aaaatcctat
      361 caacctgatg attgttgtac ccaacagcta agagcattcg ggtggcattg ggatatccat
      421 tggcccccga aagtttccag tcgtcggtat cgtgaacaag cgctgctggc tcaaccaggg
      481 caaggtttgc cccctcagcc ttggcaatat gtaaagccat ttgagttacc ctttgaacat
      541 gccaccagtc gtgcccggta gtatcgtgct caaaaaaatc ctttaccagc ctgtaaagct
      601 cggctatatt gatttgatcc atcccaattc tttgccgcta atatcgtttt tttatacgag
      661 actaagaact aatttgaagc gcctatatct attttcaaaa tttattagtc gtaaagaaat
      721 gcatccccat taggatcttc ctcaaaatcg gtagaacgac agcgcgggca gcggggtgac
      781 gcattaaacc taaactgccc gccacactta catttgccca caaattcctc aattgcagcc
      841 tcatagctat ccttcggcag aggctcaccc ggaaaaagtt ccctgattaa attatcgtag
      901 tgtgcggtag caaggctata tggctgattc aaacctttaa gataccttaa atgtaattcg
      961 cccaactgct caaagtttac agacttatgt tttccgcatg tatcgcagcg gagcatgtga
     1021 aaagtaaatc caccgccatc cattatttta aaacgtcggc cgcaattttt acaaatacca
     1081 taatactgta tacccatagc ttttaaaatt ttattttgat gacaaatatg aaatgttgaa
     1141 acgacaaatt ctgtcggtat taaaattagt ggaaattttt gccatttggc tactcccacc
     1201 cctcaagtcc atcgaaaaga gttgggccag attgaatgtt tttgcgcttg ttgctctttt
     1261 ttctgtagtt aaggaagaaa aatcgctggg catcaaaagt tgaaattgcc tttcttaccc
     1321 tgtttaccgt ttccttgctc acctcaacat tggccacccc ttttatttga gtgatttcaa
     1381 agcatcgttt agcagagttc ttctcaactt ggacagttgc tttttcatcg ccgctgacat
     1441 gcaggaaaaa cgaacgtttt gccattaatt cccgcgtata tgaggccaca cagttccgca
     1501 tttttttgcc ttcggcagca agggcctcag gcgtatcaat cagagtaatc cccttgggta
     1561 gggttaagct atcagtataa aggggttttg gtttttggct taagtttgac aaggacttta
     1621 ggtttattgc ataatcgtgg aaaccggcca ataactcgcg ggttgagtcg taactattat
     1681 tgaacctgat tgggctaaca tcacctgaac tttgaatatt ttccttggat agcaattcga
     1741 gcatttcgct cagcttttga tgtgcaatgc agcaatcagc ctttaccata cagtagtagg
     1801 ccaagttgat gtaacggaaa tactgataat ttcgggcata ctgcatagtt aacttttcaa
     1861 tacgctctag tgtagtttga gcattttgag caaccatgag gagcagtaaa gccacgttta
     1921 tcttgtccca cgagtcgaaa aagggttgcg ataacacatt cctgtaaaac atccggggcg
     1981 aaaccttttc tgacaattca tcgctaacta ttaattgtgc gagcgccaaa ggattggtaa
     2041 ctcttcgttc aataattggc ttaagcagca aagcatgtgc gaaaagaata gggtaaggaa
     2101 acgcttttac tagccaatct atcccaagcg gattacagaa tgtgtccaaa tgttcggcag
     2161 agctgaggaa actaaaattc gaaggaattg agagcactgt gcctgtggaa ttgcttacct
     2221 caaccacaaa cttttcaggc gtagtgccgt tttgcttgtt ccaaaaaatc catcgtgtaa
     2281 ggtgttgctc cttaacttta ccgtagctca aagttgattt ccccagcata aactaatgat
     2341 atgataaatt atcacccctt gcctcccaac ctaaaattag gaactaaacc gtgcgaaacc
     2401 aaggtttgaa ctatcaaatt ttaaacatgt taataattta actatattgc tgtctgtctt
     2461 tttcttatac ataacataga cctgatatcc gaaagtgaaa tgtacccaga aatcagaata
     2521 tcaaaaccac atgttgcatt tctcctgcta ataggttatt gacaaaggaa gattgaaata
     2581 atgattacat taaaagttgc gtgtttcagt tttttgcata ttacactgtg caaataaaca
     2641 ataaaatgca cagtatattg tgaaataaat taactttacc taaaaaaatg gagcagctta
     2701 acatcattta ccacgagtta acagataagg taaataccag atttactagg tatttgcttc
     2761 caaaaatcaa ctggaatgcc agacttatag ccattacagg gccaagagga gttggaaaaa
     2821 ctacactaat gctacaaaaa attaagattg aaaacaaaat agatgaaagt ctttacgttt
     2881 cgctcgataa tatttatttt tcaaaccata cattgttcga attggctggt tacttttata
     2941 ggtatggcgg ccgccattta tttattgatg aggtacataa gtatgaaacc tggtcacaag
     3001 aaataaaaaa tatttacgat agttttcccg atttgcaaat tgtgtttagc ggtagttcta
     3061 ttctcgatat ttataaaggt tttggtgatt taagcaggcg tgttgtcccc tatcatttaa
     3121 atggattatc atttcgcgaa tatttgatat tcgaaacagg gcaagactcg gaagcatata
     3181 gccttgacca aatactttct caaaaagtga aaataaaact cgaaaatcct ttgattcact
     3241 tcaaaaagta cttaaatcat gggtattacc cgttttacaa ggacgacgat tttatgattc
     3301 gattaacagg aactgtaaat gctgttttag aggtagatat tccaaaatat ttagacttga
     3361 aggtttcaac catcgataaa ctaaaacatt tgttgcaaat aatttccgaa agtgttcctt
     3421 ttaaaccaaa ttactctaaa attgctgatt taatcggggt atccagaaat ttattacctg
     3481 attatcttgc ttatcttgaa aaagcaggct taattcttga gttaaccaac gaaaccaaag
     3541 gtattagaag gctaggaaaa gtaaacaagg tatacctgaa caatccgaac ttagcctaca
     3601 tgctaagtgg tgaaaatgcc aatattggca atatcagaga aacatttttc ttcaatcaac
     3661 ttaaattaaa tcacaaagta ttttcttctg aaacagtaga ttttaaggta gaaggtaatg
     3721 aatttgaaat aggtggcaag agcaaaggca ataaacagat ttccaaactt tccaatgcct
     3781 atatagtaaa agacgacatt gaatatggtt ttggaaatac catcccgctt tggcattttg
     3841 gtttgttgta ttagtaatgg ctattttacc ccaatcccca tttctaaaca ctaactattt
     3901 cccctttgta ttcaaaatcg gctaatggta aaatgtttac tacctttgtt gccaataaca
     3961 aaagttatga ctcgacgtat tcaacccttt gccgtttaca gcgatggcaa gggaaatatt
     4021 tatgaaaatc ccaaactatt tgcagcggga cgctcgggaa accgtgttta ccccctttac
     4081 ctcgatgaaa tgatacccct gccctatggc agcgaacttt ttgaacttcc cggtcgtaag
     4141 gttttaggct attcccgaaa gggcgaactg gttgaaagca accaaggaat tgcctgcgct
     4201 gcctttattg cccctgccca cactcaactc tctattgctg catgggttaa cacacccaat
     4261 gcacctgttc taccactcta cgcctataca gctattggct ggtacaacgg gcaattctac
     4321 gtcccagcca tccgaataga ccccgatact cgacaggatt gcgagaactt caaccagaaa
     4381 atcattataa aacggggcaa ggaacttaaa ttgcgattcc caaacaatag gctaatccac
     4441 cacttggtgg ataactgcgc ctttacctac ctttgccctg cagcgcgaaa ctacgtgatg
     4501 aataggtggg aggccccttt acccacctcg cctacctgta acgcacgctg cgtgggatgt
     4561 atttccaaac aggacaaaag tgaatcgccg gtacacgagt cgcagcatag gcttaccttt
     4621 gtgcctactg tcgatgaaat agtagaagtg gccgtaccgc acctcgaaac agcacctaac
     4681 ccggtggtga gctttggcca aggttgcgag ggggaacccc tgatgcagtg ggagttgcta
     4741 cgcgatgcca ttaaggctat cagagctaaa acctcaaaag gtattattaa cctgaatacc
     4801 aatggcagta ggcccaaagc agttgaggaa ctttttcggg ctggacttaa cagcatcagg
     4861 gtaagcatga actctgctca ggaaaaatgg tacaatctct acatccgtcc ggtaaacttt
     4921 actcaagccg atgccgttga gtcgctcaga attgcccacc ggtttaacgc atggtcgtca
     4981 atcaactact ttgtaatgcc gggtattacc gatacagccg aagagctgaa tgcgctgaaa
     5041 gtaattattg aatctgcccg tattaacatg atacagtggc gcaacttcaa catagacccc
     5101 gattggcttt ttagccgcat tgaatttaat gggcaaggag aaccccttgg agtgaatcaa
     5161 ataatggaac aaatacgtat actctacccc tgggtttact ttgggtactt taacccgccc
     5221 ttaaacatta tcgaacagta tcagtttaaa tagatataga tctatggaaa cgtacatctg
     5281 taaggcttgc aatttgaagt ttgatgctaa cgggactaaa accgaatgga ttgatgccgc
     5341 atttggtcca tgcagtaagt tgatagcaaa gtgcccagaa tgcgggaacg agtgcgatga
     5401 attccgtatt cccaagcagg gttcaaccga tagctaccaa catgcttcac acaagccagc
     5461 ctgcgattcc tgctgcggtg gatgtggaat ataacgctaa aagctaaaat gatatggttg
     5521 agattaaagt tcttggtata ggctgcccaa actgccacaa gctggaggct atgtgccacg
     5581 aggtggtaaa cgagctgggg attgatgctc acattgagag cgtaacggat atgaatcagt
     5641 ttatggatta tggggtgcta ctaaccccag ccctggttat aaataacgag gttaaggttc
     5701 aggggaaatt accccttaag cccactcttg taaactggat taaacaaggg gacaaatagc
     5761 atgaatgcct gcaaaagcag gcattcagtg taaattctac caagtattca catcgtaatc
     5821 gggcaccttg cgaactcgct tggctacctc ctggcctttc tcgttgatgc caattacaaa
     5881 ctcaaccata tcgccctcaa tgagttcctc aaactcacca tcttcaacat cctggtagtg
     5941 gaagaaaagg ttgttgggcg gatactgaat gaaaccgtag ccattgttca cacttaaaat
     6001 gcgtgcgcgc ttggcttcct catcggtaat gatttcttta gtcgcctgtc ggggctggct
     6061 gtagtcctga accgcaaaga ggttctcaac caccttatcc ttacgcttta acccatcgtt
     6121 aatgagttcg tgcatgggta gggggtaagt aacctcacga agcaactcct gcgaggtgcg
     6181 cgtggttacc tcgcggcctt ggtcgtcggt gtaatcaaaa tcccacgcta aaaccataac
     6241 cttagttccc aacgagttga ttttgcggat aagcggaacg tagtcgccat cggaggtgat
     6301 aagcaccaga acgttaaagc gcttgtagag cgtttgctca aaggcttcaa gggcaagcca
     6361 aacatcaatt cctttatcct cgcggcgtcc accacgcggg cgaatgggaa ggtagtgggt
     6421 aataacccct tcggacatca ggatatcatc gaataccctg tcgttgtaga gctgatttcc
     6481 acgctggctt gcctcctggg cgctgatgcg gccacggaaa aaatgtgcct caatcagctg
     6541 gcagaaatta gggtctacac cctcctcgtt ggcaaccata tgccgaataa actcatgtaa
     6601 accggcaata ttcaacctct ttttccgctg atggaagtag ttgtagtagt tgcttacatg
     6661 caggaagtag ttcccgtcgt agaacacgcc aatgcgtatc aaatcctttc tctggttttt
     6721 tagagccatg tttaatagat tttggattgt taattaactt aattagctgc tagtctcttt
     6781 gagtgcaaag aggctgcaaa tataattctt tagcaatcac acaaaaaaaa tatatctaaa
     6841 taatgcgata tcttaatgca aacgtttaca ggtcgataat tccattttta atggcatact
     6901 taaccaaatc gacagtagtg gttaactcca
//
LOCUS       DAOOWJ010000124         6927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_10990_length_6927_cov_7.991269, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000124 DAOOWJ010000000
VERSION     DAOOWJ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10990_length_6927_cov_7.991269"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..4114)
                     /locus_tag="PLW22_10305"
     CDS             complement(86..4114)
                     /locus_tag="PLW22_10305"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU75134.1"
                     /translation="MENPIVIGILENAAILIASAVLYDFIWLKSDKSRKIRTQILLGL
                     LIAGIGIVLMNSPWILVEGIVFDTRSVLLTISGLFFGGVTTVVAAVIVATSRLMMGGA
                     GMYMGVAVIVSSSLISLLWRKVRPDIIANKNLGEVYLVSIIVHLVMLGCTIFLPEENR
                     MATLLVIWWPVILIYPLATVLIAKLLFSRKENWKQKDKLKESEERYRLIFENNPNPMW
                     IQDLETLNFLEVNRAAITHYGYTREEFLNMTLKDIRHEDDLSKLNGDIQALDGIYQTS
                     FSCIHKKKNGEIIHVQVASHRITYKGRPAKHVMVSDVSELVKSHKLLSQKEQEFREIF
                     NSGFDSIQIVDTESGKIIDCNNITMEMYGFTKEEIINGIIGDLSANIEPFTKEKAQEY
                     IKKTIKGEPQDFEWLARKKNGETFWVNVRLKLVEIGGVNRVMAIIRDISDRKRTEQNF
                     IESEQRFLRVMHFSKDAIGILDENFHFIDCNDSAARLHGFKSRIELLKNSPHPSTLSP
                     QTQPCGSSSYELANKMIEIALEQGFNKFEWLHKKVNGQKFWAEVSLTPIVFNGKRMIY
                     AVWRDISDQKNKQNRIERLLKILDESINEVYIFRCDNLKFIEFNKTAVNNLGYSPEEL
                     LELTALDLKPYLTAEKFNELLKPLNDGTSSKIYFETIHRRKNGTEYPVEVYVEKTKAS
                     DIDVYNAIVLDISQRKKYLAELKQREHELREIFDSTLEAILIYDIETSKIVDCNRQTQ
                     TLYGYSRDELIGMPLGTLSANDEKYNIDEATKWAEKALTVGPQTYTWLGKKKSGETFW
                     VEISLKKSTIGGVDRLMAVVRDITERIAHQHEIERREKEYSEIFNATAEAILIYDPHA
                     NLIVDCNNRAVEMYGYNDKRGFLSIGLNALGANVEPYTNDKFGYYTQKAAKEGPQRYD
                     WLAKRKNGEHFWIEVNLRLTKIGGVERELIVIRDITDRKKFEDDLVKAKEKAEESDRL
                     KSAFLANLSHEIRTPMNAIMGFGDLLKYSQDEQKRKEYIDIIQKSGFRLMDIINETVE
                     IAKLDTGLIKTNPETFSLDQLMQDIYNELKIKISGSRELELIYLPPNTTSETYIYTDR
                     VKLQQILINLITNGIKYTPRGAVAFGYEVKDNRVVFNVKDTGIGIDRKNREMVFKRFY
                     RVENPLTIKVGGIGLGLAITKAYTELLGGSIKLDSELGKGTSVTVTLPFMTGEPKSEI
                     FVELPQEQLKGENELILVAEDDEYNFLYINEILREFNYKCIRAANGEEAVNIAHNNEN
                     IKLILMDLKMPTMDGFKAFEEIRKFKPQIPIVAQTAYVLGEDKVKIETYGFDGYLTKP
                     LKKDELLRMVNEKLKRELKDKG"
     gene            4637..6217
                     /locus_tag="PLW22_10310"
     CDS             4637..6217
                     /locus_tag="PLW22_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOU75135.1"
                     /translation="MIFEDEVKRRRTFAIVSHPDAGKTTLTEKLLLFGGAIHIAGAVK
                     SNKIKKTATSDFMEIERQRGISVSTSVMEFEYQNYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVIDHAKGVEAQTRKLMEVCRMRKTPVIIFINKLDRPGNDPFDLLDEIEKELQ
                     IKVRPLSWPISQGPSFKGVYNLFEQKLFLFSGENKQTIADDIVEISDLNSPELDSYIK
                     PYTNKFLEEIEIVNEVYPEFDIKTYLSGEVAPVFFGSALNNFGVKELLDCFVQIAPHP
                     GPITTDTRTVTPFEQKFSGFVFKIFANMDPNHRNRIAFLKICSGRFERNVPYFHVRLG
                     KSFRFSSPNAFLADKKSIVEFAYPGDIIGLPDSGNFKIGDTLTEGEVMNFKGIPNFAP
                     ELFRYIENADPLKYKQLAKGIEQLTDEGVAQLFTQKSNGRKIIGTVGALQFDVIQFRL
                     EHEYGAKCRYEPINLYKACWIETNNKAQLDDFRIRKYNNLALDKHGREVFLADSPYSL
                     QMAIEKFPDIKFHFTSEF"
BASE COUNT         1942 a         1508 c         1237 g         2240 t
ORIGIN      
        1 attatttttg tccatacaca taagtttgtg tagacatttt tcaggacggg tcacctttta
       61 tcctttatcc tttaaccttt atcctttaac ctttatcctt taactccctc ttcaactttt
      121 cgtttaccat tctcagtagt tcatcttttt taagtggctt agttaggtaa ccatcaaaac
      181 cgtaggtttc aattttgact ttatcttcac caagcacata agcggtttgg gctactatgg
      241 gtatttgggg tttaaatttc cttatttcct caaaggcttt gaatccatcc atagtgggca
      301 ttttcaagtc cattaggata agtttgatat tttcgttatt atgggcaatg tttacagctt
      361 cctccccgtt tgcagctcta atgcatttgt agttgaattc acgtagtatt tcatttatat
      421 acagaaagtt atactcatcg tcttcagcaa ctagtataag ctcattctcc ccttttagct
      481 gttcttgtgg taactccaca aatatctcag atttaggttc accggtcatg aagggtaatg
      541 ttacagttac cgatgtgcct tttccgagtt ccgagtccaa ctttatgctt ccacccaaaa
      601 gctcagtgta agcttttgta atggcaagcc caagcccaat tccacctacc ttaattgtta
      661 agggattttc aaccctataa aaacgcttaa aaaccatctc cctattctta cggtcaatcc
      721 caatgccagt atccttaacg ttgaacacca cacggttatc ttttacttca taaccgaatg
      781 ccaccgctcc acgaggggtg tattttattc cgtttgttat caggtttatt agtatttgtt
      841 gcagcttgac cctgtcagta taaatataag tttccgaggt tgtgtttggg ggtaaataga
      901 ttagttcgag ttccctactt ccagatattt ttattttcag ctcattgtaa atatcctgca
      961 ttagctggtc aaggctaaag gtttcaggat tggttttaat tagcccggta tcgagtttgg
     1021 ctatctcaac agtttcatta ataatatcca ttaaacggaa gccgcttttc tggataatat
     1081 caatatactc ctttcttttc tgttcatcct gtgaatattt taaaagatcg ccaaacccca
     1141 ttatggcgtt catgggtgtt ctaatttcgt gcgacaggtt ggccagaaag gccgacttta
     1201 acctatcact ttcctcagct ttttcttttg cctttactag gtcatcttca aatttttttc
     1261 ggtcagtaat atcgcggatt actataagtt cccgttcaac acctccaatt tttgtaagac
     1321 gaaggtttac ttcaatccag aaatgctcac cgtttttacg ttttgcaagc caatcgtacc
     1381 gttgggggcc ctccttggct gctttttggg tataatatcc aaatttgtcg tttgtgtatg
     1441 gctcaacatt ggctccaagg gcattcaacc caatgctcaa aaaccctctt ttgtcattat
     1501 acccatacat ttcaactgcc ctattgttac aatcaactat aaggttagca tgtgggtcat
     1561 aaatcagaat tgcctcggca gtagcgttga atatctcgct gtactccttc tcgcgtcttt
     1621 caatttcatg ctggtgggca atacgttcgg taatatcgcg cactacagcc ataagtctat
     1681 caacccctcc aatggttgac tttttgagcg atatctctac ccagaatgtt tcgccgcttt
     1741 tctttttacc cagccatgtg taggtttgtg gtccaactgt caacgctttc tcagcccatt
     1801 tagtagcttc atcaatattg tacttttcat cgtttgcact gagggtacct aatggcatac
     1861 caattagctc atccctgctg tatccgtaaa gagtttgtgt ttgcctatta cagtcaacaa
     1921 ttttactggt ttcaatgtcg tatatcaaaa tggcttcaag ggtagagtca aaaatttcgc
     1981 ggagttcatg ttcgcgttgt ttcaattcag ccaaatattt ttttctttgg ctaatatcta
     2041 agacaattgc gttgtaaaca tcgatatctg aagcctttgt tttctcaaca tagacctcaa
     2101 cgggatattc agtaccattc ttacggcgat gtattgtctc aaaatatatc ttagatgaag
     2161 ttccgtcgtt aagaggtttt aacaattcgt taaatttttc agctgttagg tatggtttta
     2221 aatcaagtgc ggtaagctca agtaattcct ctggtgaata acctaagtta ttcacagcag
     2281 ttttgttaaa ttcaataaat tttagattgt cgcatcggaa aatatatact tcgtttatac
     2341 tctcatccaa tatctttagt agtctctcaa ttcgattttg cttgtttttt tgatcagata
     2401 tatcgcgcca tacagcatat atcatccgtt ttccattaaa tacaattggt gttagcgaca
     2461 cctctgccca gaatttttgt ccatttactt ttttatggag ccattcaaat ttgttaaaac
     2521 cctgttcaag tgcaatttca atcattttgt tggctagctc atacgatgaa ctcccacaag
     2581 gttgagtttg tggacttaga gtcgaagggt gaggtgagtt tttaagcagt tcaatacgcg
     2641 atttaaaacc atggagtcgt gccgcagagt cattacaatc aataaagtga aaattttcat
     2701 ccagtattcc aattgcatcc tttgagaaat gcattacacg taaaaaccgt tgttcacttt
     2761 caataaagtt ttgctctgtc ctttttctat ccgaaatatc tcttataatg gccattaccc
     2821 tattaacacc tccaatttca actagcttga gtcgcacgtt aacccaaaat gtttccccat
     2881 tttttttacg tgctagccat tcaaaatcct ggggttcgcc tttaatggtt ttttttatgt
     2941 actcttgagc tttttctttg gtaaagggtt ctatgtttgc acttaaatca ccaataatcc
     3001 cattaataat ttcttccttt gtgaaaccat acatctccat tgtaatattg ttacaatcaa
     3061 ttatttttcc actttcggtg tcaactattt gaatggagtc aaatcctgag ttaaatatct
     3121 ccctgaattc ttgttctttc tggcttagta atttatgcga cttaaccaat tcacttacat
     3181 cgctaaccat gacatgtttc gctggtcttc ccttgtaagt aattctatgc gatgctactt
     3241 gaacatgtat aatttcaccg ttctttttct tatgtatgca gctaaaggat gtttgataaa
     3301 taccgtcaag ggcttgtatg tcaccattca attttgaaag gtcatcttca tgtctgatgt
     3361 cttttagagt catgtttaaa aactcttccc tggtataacc gtaatgagta atggcagccc
     3421 tattaacttc caaaaaattt agtgtttcaa gatcttgaat ccacatggga ttgggattgt
     3481 tttcaaaaat caaacggtat cgctcctcgc tttccttaag tttatctttc tgtttccagt
     3541 tttccttcct actaaaaagt agctttgcta ttaggactgt agctaaaggg taaattagta
     3601 ttactggcca ccatattaca agcagggtgg ccattctgtt ttcttcgggt aagaaaattg
     3661 tgcatccaag catcaccagg tgaactataa tgctaaccaa gtaaacctca ccaaggtttt
     3721 tattcgcaat tatgtctgga cgaactttcc gccaaagcag gctgataagc gatgaactta
     3781 ctattactgc cactcccata tacatcccag caccacccat catcaatcgg gatgtagcaa
     3841 caattaccgc tgcaaccact gtggttaccc cgccaaaaaa cagtcccgaa atggtgagca
     3901 ataccgatcg ggtatcgaat actatccctt caactaatat ccatgggcta ttcataagaa
     3961 ctatacctat tcccgcaatg agtaggccaa gcaaaatctg tgtgcgaatc tttctacttt
     4021 tatcggactt taaccaaata aaatcgtata gaactgcaga tgcaattagt attgctgcat
     4081 tttcaagtat tccaataact atgggatttt ccatggatgt aaaaaatttg gtatcaccat
     4141 aaatttaggc aatattttat cttaaatcaa taatcaataa tcaataacca atattcttta
     4201 gttattgata tatgtcatat tgagtcctga tataccaaga aatactccat tgaaacatct
     4261 ctcttttatg ctgaaccctg ccgaagcatc actttacctt tataacccat taatggatcc
     4321 ctggctgcga tctgaatgac aaaaggattt gacggttgat tgactcaaaa ttgaacgcaa
     4381 attgcactta ttaagttgat ttacacaggt taaactaacc aaacccaaaa cactaaacac
     4441 caaacacaat cacccctgta cactgaacac tgcacactat aaataaaatc cctcgcaacg
     4501 ctcagaatga ttaaagggat gtaaactatt taaggctaat catcataaat catcaaccct
     4561 tactttcttt ttcttcgcac cttaattcat ttaaaggcta ataattgcta attttgcaag
     4621 ccataaatga agatatatga tttttgagga cgaagttaag cgccgccgaa catttgccat
     4681 tgttagccat cccgatgccg gtaagacaac cctaaccgaa aagttgctgc tctttggcgg
     4741 agcaattcac attgcaggtg ccgttaaaag caataagatt aaaaagactg ccacgtccga
     4801 tttcatggag atagagcgcc agcgtggtat ttcggtttcc acatcggtaa tggagtttga
     4861 gtatcagaac tataaaatta acattctcga tacacctggt caccaggatt ttgccgagga
     4921 tacatatcgt acccttactg ctgtcgatag cgttataatt gttattgacc atgcaaaagg
     4981 cgttgaggcg caaacccgca agctcatgga ggtttgccgc atgcgtaaaa ctcccgtaat
     5041 catctttatc aacaagctcg ataggcctgg caacgaccca ttcgacctgc tcgatgagat
     5101 tgagaaggaa cttcagatta aggtacgccc gcttagctgg ccaattagcc aggggccatc
     5161 gttcaagggg gtttacaacc tttttgagca gaaactcttc cttttctccg gtgagaataa
     5221 gcaaaccatt gccgacgata tagttgagat atctgatttg aactcgcctg aactggattc
     5281 ttacattaag ccctacacca ataagtttct tgaggagatt gagattgtta acgaggttta
     5341 cccggagttc gatatcaaaa cctacctttc cggcgaggtg gctcccgttt tctttggtag
     5401 tgccctaaac aactttgggg ttaaggaact cctggattgt tttgtacaaa ttgctcccca
     5461 tcccggccct atcactaccg atacccgtac ggttacacca tttgagcaaa aatttagcgg
     5521 atttgtgttt aaaatttttg ccaacatgga tcccaatcac cgtaacagga tagcattcct
     5581 aaagatatgc tctggtcggt ttgagcgtaa tgttccatac ttccacgtaa gattaggaaa
     5641 atcgttccgt ttttccagcc ccaacgcatt cttggccgat aaaaagtcga tagtggagtt
     5701 tgcatacccc ggcgacataa ttggcttgcc cgattcaggc aatttcaaga ttggcgatac
     5761 ccttactgag ggcgaggtta tgaactttaa aggtatccct aactttgccc ctgagctttt
     5821 ccgctacatt gagaatgccg accccttaaa gtataagcaa ctggcaaaag gaattgagca
     5881 gctaaccgat gagggtgtag cccagctctt cacccaaaaa tccaatggta ggaagattat
     5941 aggtacagtg ggtgctttac agtttgatgt tattcagttc cggttggagc atgagtatgg
     6001 tgctaaatgc cgctacgaac ccataaacct ttacaaggct tgctggattg aaaccaataa
     6061 caaggcccaa ctcgacgatt tccgtattcg aaagtataat aaccttgccc tcgataagca
     6121 tgggcgtgag gtattcctgg ccgattcacc ttactcccta caaatggcta ttgaaaagtt
     6181 ccccgatatt aagttccact tcacttcaga gttttaatat aggttgttag ttgtttgttg
     6241 atggttgata gttattacaa aatgtttgac acagtaaagt actgtgaaac cctgtattaa
     6301 tttgtgatac ctgataaact ttattgagga aattttttca ggtttaccgt gaacagtcct
     6361 gtaggagcta aacatttata actccattgt cattccgagc aagtcgagga atctcattaa
     6421 atacgggatg atagcacata gagatgcttc ggcaagctca gcatgacacg ataggacggg
     6481 gttacaccaa actatcaaca atcaaacaac aaccatcaac ttaaatcgtt tcacagggta
     6541 aatccaccca atctgcgtca tctgcattcc attgcattag caagtatcac agagttacac
     6601 agtgtaacac ggtgtttcac agaattgcaa tcacgaacaa gcatcaactt tatatttgtc
     6661 atcctgagcg cagcgaagga tctctaatcg acacattgag atgtttcgct tgcgctcaac
     6721 atgacaatag tttcacgagc aacgaacaac gctcccagcc ccgtaggggc gtaatatttg
     6781 taacacaata gttactccct catgcatagc gatgcacgcc atggcattcc aaagagcaag
     6841 ggtgaaacat tgcatcgcaa aagaaaaaac agggcaaaga gcggtttaca tgttctttct
     6901 tgtcttgata caagaaagaa ccaaaga
//
LOCUS       DAOOWJ010000125         6851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_11142_length_6851_cov_7.077987, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000125 DAOOWJ010000000
VERSION     DAOOWJ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11142_length_6851_cov_7.077987"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(502..2394)
                     /gene="speA"
                     /locus_tag="PLW22_10315"
     CDS             complement(502..2394)
                     /gene="speA"
                     /locus_tag="PLW22_10315"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOU75136.1"
                     /translation="MRKWRIEDSAELYNINGWGVKYFSINEKGNVVVTPKSNGVEIDL
                     RELVDELQVRDVAAPMLIRFTDILDNRIEDMSRSFKQAAEEYGYQGKNFIIYPIKVNQ
                     MRPVVEEIINHGKKFNIGLEAGSKPELHAVIASNPDNESLIICNGYKDEDYIELALLA
                     QKMGKKIFIVVEKLNELKVINKVSQRLKIKPNLGIRIKLASSGSGKWEESGGDVSKFG
                     LTSSELLQALDFLDKNDLKGSLKLVHFHIGSQVTKIRRIKIALREASQFYVQLRLMGY
                     NVEFVDIGGGLGVDYDGTRSSNSESSINYSLQEYVNDSVSTLVDASDKNNIPHPNIIT
                     ESGRSLTAHHSVLIFEVLETASLPSWDEDDELPADAHELVVELYEQWSKLNQSRMLET
                     WHDAQQIREEALDLFSLGMLDLKTRAQIEKLFWSIARETFEMTSQLKHIPDELKSLPK
                     LLADKYFCNFSLFQSLPDAWAIDQVFPIMPIQRLNEKPDRVATIQDVTCDSDGKIDNF
                     ISTKNISYFLPVHSIKSNDSYYIGVFLVGAYQEILGDLHNLFGDTNAVHVSVDDEGYS
                     IDQIIDGETVAEVLEYVQYNPKKMVRTLETWVTSSVKAGVISPKEGKEFLSNYRSGLY
                     GYTYLE"
     gene            complement(2563..4497)
                     /locus_tag="PLW22_10320"
     CDS             complement(2563..4497)
                     /locus_tag="PLW22_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU75137.1"
                     /translation="MQATRYIIVILTLVLCQLSVNAQSVKKKYTISNKRVIEQYEKAL
                     SSFDRYDYITAKKLLVECVQKEPKFIEAYLVLSQVYMGMGELNLAIESILKAQSIDKD
                     FHPRAYYILGLLYFSTGEYQKALNSFETFLTYKDKRATMNDSARKMIEKCQFALNQIA
                     NPVPFVPKNIGEGVNSDLDEYWPSLSVDERTLVYTVRLPKNPDKGIKGTKWQEDLYIS
                     YRNEDGQWGRGEPVKSPLNTEFNEGAQAVSSDGKTIYFTICRGVCNLYQSVLEPDGFW
                     SLPQKLPAAINSERYSEKQPSISPDGKTLYFVSNRPGGLGKFDIWKSELKPDGTWGNP
                     VNLGDVINTNESEQSPFIHFDNKTLYFSSSGHMGMGGHDIFVSRLDSNGAWTKPVNLG
                     YPINTHRDEDGLIVNARGNIAYYSTDIKPENGRDIFTFELYPEVRPTPTSYVLGTVRN
                     SKTLEPVEASIKLVDLKLDRVVMDIVSALKGEFLVCLPVSNRYGLFVSASGYLFHSEH
                     FDLTEYYSFDKPLKLDVLLQPIEKDEVITLRNIFFAFDSYELLPESRPELNYLLDILK
                     RNPEMRIEISGHTDDTGSAEYNLKLSEQRAKAVADYLISQGIDSARLKWVGYGKSKPV
                     APNSTPEGRTLNRRTEVKIL"
     gene            complement(4680..5492)
                     /gene="pgeF"
                     /locus_tag="PLW22_10325"
     CDS             complement(4680..5492)
                     /gene="pgeF"
                     /locus_tag="PLW22_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOU75138.1"
                     /translation="MPLIYKDILYFKFDNLMQYQSAITHFVSSRIGCWDDEDFTIGLN
                     GYLDNKIVLRNREYLATKFGFKAADFVFAEQVHGVKVEVVTPMQKGNGAFSKNDALQH
                     SDAWITNYPGICLVAQAADCVPILFYDPVNNAIGAAHAGWKGTVNRIAANVVKSMISE
                     YGTNPANLLVGIGPSAGPCCYEVGDDVASVVNKEFPQKYDLLLYYPDREKPVLDLWRA
                     NLYALLEVGVPVGNIELAGLCTICNNHVFFSARCGDRGRFGGAIMLNVLGQN"
     gene            complement(5614..6690)
                     /locus_tag="PLW22_10330"
     CDS             complement(5614..6690)
                     /locus_tag="PLW22_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75139.1"
                     /translation="MKSIRFFPVVLLLLVANSVLAKVIPQTREYSKEFQVTDKSKLVV
                     ENRFGQVNIQNWDKSAISIYVQVKVDNSNATRAKALLDAIEVELSQEGDVVKAITSFN
                     EEFIRSNRRMFEMSSDELSIDYTIKMPKNVDVQIENKFGDIYINELNSHLAVELKYGN
                     IKIDKLTRGDSESLNTIDVSYGNASIVEANWIKTQLKYGKLEVQKLVAGVVLSRYSKI
                     SLSNVSSLVLDSKYDKYEIGQVNNLVGESGYTVFNIDVLNKKFNLNAKYGDVKIASVD
                     ANFENISFNAAYTGLKASIPSGTSYLLNANVSFGKVDIGNVPSKLNRIEGNTTVELSG
                     VVGESANPKARVEINMKYGNATLY"
BASE COUNT         1927 a         1590 c         1327 g         2007 t
ORIGIN      
        1 taggcccacc gccacgttcc acccccgacc cattgccggg tcaggctttt atgccactgc
       61 gtatggtaca ccgtgttggt tgaggaatcg cattaaagaa gagttgaagg tactaagaga
      121 tgcttcgact gggctcagca tgacatgaag actgaattct gtttttacta acaacaaaca
      181 attgtctgct atcaactaaa ttactttcaa attgttatgc agtgtaaatc ttaacaatct
      241 aagtgttctg aattaactaa ctcatcgagg caaaaaatag gaacactgta agctttttga
      301 agagattagt caatacatac acaataaaaa ttacataatt atcgagataa tcccgattta
      361 tcaaaatctt tcattccgaa gttacgagat gattagattt tattagtcga ttcttcaatt
      421 gattttttca actaacgaac ttgaaaccag aaaagaaaaa agcccaccta tgtgggcttt
      481 tatatcctat tctatgcaaa tttactcaag gtaagtgtac ccatataagc ctgagcggta
      541 attggacaag aattcctttc cttccttagg cgaaataacc ccagctttaa ccgagctggt
      601 aacccaggtt tccagggtac gaaccatttt ctttgggttg tactgtacat actccaacac
      661 ctcagctaca gtttcaccat caattatttg atcaatgcta tacccttcat cgtcaaccga
      721 tacgtgaaca gcattggtat cgccaaacag gttgtggagg tcacccagta tttcctggta
      781 agcacctaca aggaatactc caatgtaata ggaatcgttg gacttgatgg agtgaaccgg
      841 taggaaatac gaaatatttt tggttgaaat aaagttatca atcttaccat cggagtcgca
      901 ggttacatcc tgtatggtgg caaccctgtc gggtttttcg tttaaacgct gaataggcat
      961 aatggggaac acctgatcga tagcccaggc atcgggcaac gactggaaca gcgaaaaatt
     1021 acagaaatac ttatcggcta agagtttagg caacgatttt agctcatcgg gtatatgctt
     1081 aagctgtgag gtcatctcaa aggtttcgcg ggcaattgac cagaaaagct tttcgatttg
     1141 cgcacgggtt ttaaggtcga gcatgccaag gctaaacagg tcgagtgcct cctccctgat
     1201 ttgctgggca tcgtgccaag tttcaagcat tctggactgg tttagcttgc tccactgctc
     1261 ataaagttcc accaccagtt catgggcatc ggccggtagc tcatcatcct catcccaaga
     1321 gggaagcgaa gcagtttcca gtacctcaaa aatgagcacg gagtgatgtg cagttaacga
     1381 cctgccggat tcagtaatga tattggggtg cggaatgtta tttttatcgc tggcatcaac
     1441 cagggttgat accgagtcgt ttacgtactc ctgaaggcta tagttaatgc tactctcact
     1501 gtttgatgag cgggttccat cgtagtcaac acctagtcca ccaccaatat caacaaactc
     1561 tacgttatag cccattaagc gaagctgaac gtaaaactgc gatgcctcgc gcagggcaat
     1621 ttttatacgc ctgattttgg ttacctggct tccaatgtgg aagtgaacca gctttaagct
     1681 accctttagg tcattcttat ccagaaaatc cagagcttgt aggagctcac tggatgttaa
     1741 cccgaacttg ctgacatcgc cacctgactc ctcccatttg ccactaccgg agctagccaa
     1801 cttaatccta atgccaaggt ttggcttaat tttaagtcgc tgtgagactt tattgattac
     1861 cttcagctca ttgagctttt caactacaat aaatatcttt ttccccatct tttgggcaag
     1921 cagagccagc tcaatgtaat cctcatcctt gtaaccgttg cagataatca gcgactcgtt
     1981 atcggggtta cttgcaataa ctgcatgaag ttctggcttt gagccagcct ccagtccaat
     2041 attaaacttt ttaccgtggt tgataatctc ctcaaccaca gggcgcatct ggttaacctt
     2101 aatggggtaa atgatgaagt ttttaccctg atagccgtac tcctcggctg cctgcttgaa
     2161 cgagcgcgac atatcctcga ttctattgtc gagaatatcg gtgaaacgaa ttagcatggg
     2221 cgcagccaca tcgcgaacct gtaactcatc aaccagttcg cgcaggtcaa tctccacgcc
     2281 attacttttt ggggtaacca ctacatttcc cttttcgtta atcgaaaagt actttacccc
     2341 ccagccgttg atattgtaga gttcggctga atcctcaatg cgccattttc tcatctctct
     2401 ttaccagata ttgctaagtt aaacatctat catacagtat cttgctatta aacttttaag
     2461 gcttgcgaag ttatattgtt atttcaacaa ttacaataca gtaatgagaa atatggttca
     2521 aaaccgttaa ataatgtcaa ctcatataaa accataagcc acctaaagaa ttttgacctc
     2581 cgttctgcgg ttcagcgtcc taccctcagg cgtactgtta ggggcaaccg gtttgctttt
     2641 accatatcca acccacttca gccttgccga atcaatccct tgcgaaatta ggtagtcggc
     2701 cactgctttt gcgcgctgct cggatagctt caggttgtac tcggcacttc ccgtatcgtc
     2761 agtgtgcccg ctaatctcaa tcctcatttc ggggttacgt ttaagaatgt ccaacaggta
     2821 gtttagctca ggtctcgatt cgggtaatag ctcgtacgag tcaaatgcaa aaaagatatt
     2881 cctgagggtt atcacctcat ccttttcaat gggttgcagt agaacatcaa gctttaatgg
     2941 cttatcgaaa gagtagtatt ctgtcaaatc gaaatgctcg gaatggaaaa gataccccga
     3001 tgccgaaaca aacaaaccgt acctattgct tactggtagg caaaccagga actcaccctt
     3061 caatgcggaa actatatcca ttacaaccct atcaagcttt aaatcgacca gcttaatgct
     3121 cgcctcaact ggctcaaggg ttttactgtt tcgcacagta ccaagaacat acgatgttgg
     3181 agttggacgc acctcgggat aaagttcaaa ggtgaaaata tcccgtccat tttccggttt
     3241 gatgtcagtg gaatagtagg ctatgttacc cctagcattt acaattaagc catcctcatc
     3301 gcgatgggtg ttaattggat aacccaggtt gaccggcttt gtccaggcac cgttactatc
     3361 gagccgactt acaaagatat cgtgaccgcc catccccatg tggcccgacg atgaaaagta
     3421 tagagtttta ttatcgaaat ggatgaaagg cgattgttca ctctcgttgg tattaatcac
     3481 atcacccaaa ttcaccgggt ttccccaggt accatcaggc ttaagctctg acttccatat
     3541 atcgaactta ccaaggccac ccggcctatt acttacaaag tatagcgttt taccatcggg
     3601 ggatatggaa ggctgctttt ccgaatacct ttcagaattt atggcagctg gtaatttctg
     3661 cggcaaactc caaaatccat cgggttctag aaccgattgg tataaattac atactccgcg
     3721 acaaattgta aagtatatgg tcttgccatc ggacgaaacc gcttgggctc cttcattaaa
     3781 ctcggtgttc aggggtgatt ttacaggctc tcctcttccc cattgaccgt cctcatttct
     3841 gtaggaaatg taaaggtctt cctgccactt ggttcccttg attcccttat cgggattctt
     3901 gggcaagcga acggtataaa ccagtgtacg ctcatcgacc gaaaggcttg gccagtactc
     3961 atccaaatcg gaattaaccc cttcgcctat attttttggt acaaaaggaa ccgggttggc
     4021 aatctgattt agggcaaact ggcacttctc aatcattttg cgtgccgaat cgttcatggt
     4081 tgccctttta tctttgtatg tcaaaaaagt ttcgaagctg ttgagcgcct tttggtactc
     4141 ccctgttgaa aagtaaagca accccaaaat gtagtaggct cttggatgaa aatctttatc
     4201 gatgctttgg gctttcagaa tactctcaat ggctaagtta agctccccca ttcccatgta
     4261 aacctgggaa agcacaaggt atgcctcaat aaactttggc tctttctgaa cacattcaac
     4321 tagcaacttt ttagctgtta tataatcgta acggtcaaat gaactcagag ctttttcgta
     4381 ctgttcaatt actcgtttat tactaattgt atactttttc ttgacactct gtgcatttac
     4441 actcagctga caaagcacta gggtaagaat aactataata tatcgtgtag cctgcataac
     4501 ttacctctat attattttga tacgaaataa aggtaatcat tgttttcgaa aatgacacca
     4561 agttgatagc ttagaactta tgcgtgaaaa atttaccaga acctaaagtg atatcctcct
     4621 ggctattcaa gatgtacagt tcaacccaaa ccacttccag caataagaat agaaaaaggt
     4681 taattctggc caagcacatt aagcattatg gcaccgccaa acctacctct atcaccacag
     4741 cgagccgaga agaatacatg gttattgcaa atggtacata aaccagctag ttcaatatta
     4801 cccacaggta caccaacctc taataatgca taaaggtttg cacgccaaag gtcaagaaca
     4861 ggcttttccc tatcgggata gtatagtaaa agatcgtact tttggggaaa ctctttatta
     4921 acaactgaag caacatcatc gcccacttcg tagcagcatg gccctgccga agggcctatt
     4981 cctactaaaa ggttggcggg gtttgttccg tactcgctaa tcatagactt taccacattt
     5041 gctgcaattc gattaaccgt tcccttccag cccgcatggg ctgcacctat tgcattattc
     5101 actgggtcgt agaaaagaat gggaacgcaa tcggcagcct gggctacaag acaaattccc
     5161 gggtagttgg ttatccatgc atcggaatgc tgaagggcat cgtttttgct aaaagcccca
     5221 ttcccctttt gcattggcgt aacaacctca accttaactc catgcacctg ctcagcaaaa
     5281 acaaaatcgg ctgctttaaa tccaaacttg gttgcaaggt actccctatt cctaaggaca
     5341 attttattgt ccaagtaacc attcaagcca attgtaaaat cctcatcatc ccagcaacca
     5401 atgcgcgaag aaacaaagtg agtgattgcc gattggtatt gcattaaatt atcgaacttg
     5461 aagtacaaaa tatctttgta gattaaaggc attcgctgtt ttttggtatg taacaaaagt
     5521 aactaaaaat cggccaccaa taaaaaataa aatccgacga actgtcggat tttagcacct
     5581 aactgttagg ttttgggtat gaatggtggg acactagtag agcgttgcat taccgtactt
     5641 catgtttatt tcaacccttg cctttgggtt agcggactcg cctacaaccc ccgatagctc
     5701 aaccgttgta ttcccctcaa tacgattaag tttacttggt acgttaccaa tatcaacctt
     5761 cccgaacgac acatttgcat tcagcaaata agatgttcca gatggaattg aagcttttaa
     5821 tccggtatag gcagcgttaa agctgatgtt ttcaaaatta gcatctaccg atgcaatctt
     5881 aacatcacca tacttggcgt tgaggttaaa ctttttattg aggacatcga tattaaaaac
     5941 cgtatatccc gattcaccaa ccagattgtt tacctgtcca atttcatact tatcgtactt
     6001 ggaatccaat actaacgaac ttacgttcga caggctaatt tttgagtatc gcgatagaac
     6061 aacccctgca accagtttct gtacttcgag tttaccatac ttcaactggg tcttaatcca
     6121 gttggcctca acaatagatg cattaccata gctgacatca atggtattga gggattcgga
     6181 gtctccccgg gttagcttat caatcttgat attaccgtac ttcagctcta ctgcaagatg
     6241 gctgtttaac tcattgatat agatatcgcc aaatttattc tctatctgaa catcaacatt
     6301 cttgggcatt ttaattgtat agtcaatgga caactcatcg ctcgacattt cgaacattct
     6361 gcggtttgac cttatgaact cctcattgaa tgaggtaatg gcctttacca catcaccttc
     6421 ctggctaagc tcaacttcaa tggcatcgag taaggctttg gccctggttg cgtttgaatt
     6481 gtcaacctta acctgcacgt aaattgatat agcactttta tcccagttct ggatgtttac
     6541 ttgaccaaac ctattctcaa ccaccaattt ggatttatcg gtaacctgga attccttgct
     6601 atactccctg gtctgaggga ttacttttgc taatacgcta ttagcaacaa gtagcaacag
     6661 aacaaccgga aaaaacctaa tacttttcat attgtttgag tttaatgatt tctacttttt
     6721 tactctcgag cctgttttaa agcttgttca acgcgttcca tggtttccag tcgaatctgg
     6781 taactctgaa tgattgcgcc caaggcaaca tcgctccttg gattagctga tatctcagat
     6841 tttagcaggg g
//
LOCUS       DAOOWJ010000126         6830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_11183_length_6830_cov_5.979779, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000126 DAOOWJ010000000
VERSION     DAOOWJ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11183_length_6830_cov_5.979779"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            578..2599
                     /locus_tag="PLW22_10335"
     CDS             578..2599
                     /locus_tag="PLW22_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU75140.1"
                     /translation="MDEVEINAILAELDDLRKKYEELKALHDAQNKIQSDIESRLLDS
                     EARNAALLSANPDMMFVFDKQGYFIDYNGGLNEMYAPPEFFVGKRVDEVLPAPIATLT
                     HEKIKQLFETGKMQVYDYDLVIQGKTMFFESRLVKYGTDKALAVVRNITQRKNYEITL
                     KESELRYQELYNLLRLLADTAPDMIWAKDAQKRYIFANRAMCQELLNASDTNEPVGKT
                     DLYFALREREKHPEDPNWHTFGELCMDSDEITMRELRPMQFDEYGNVKGKFMFLDVHK
                     APIFSNDGKLMGVVGTARNITEKKEMEEKLKLSEQTYKGILNSISEAVYIQDENGVFI
                     DVNDTATRIYGYPREYFIGRTPEFLSAPGYNNLEQVAQCVKKAFHGEPQVFEFWGIRH
                     DGAYFPKIVSLTPGVYFGKNCVIAVARDITEQKNWEKEILEAKSKAEENDKLKSAFLA
                     NMSHEIRTPLNGIMGFVQLLRNPNLSKDDLDKYLNVIERSGERLNELLTGLLDMARIE
                     AGQVKVIYKRTNIASLVQYVTEFFTPDARVKGLGITHKINIPEQLVEIETDGYLLQDI
                     FFNLIKNAIKYTNKGNITIGCEYFDSSLLFKVSDTGIGVPRDKQKVIFDRFVRANNDL
                     TNPVDGAGLGLSIAKAYVERLGGKIWLESEPGRGSTFYFTIPLSPNVNF"
     gene            complement(2879..4984)
                     /locus_tag="PLW22_10340"
     CDS             complement(2879..4984)
                     /locus_tag="PLW22_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU75141.1"
                     /translation="MIRALKNTFLFFATLIISGCSLVPTAPLAEQHDTLLVMFGDTRI
                     DPYFWLRNRESQKVIDHLLAENAYTYAVMEPTLSLQDRLVAEMRSRKRGDDITVPVFM
                     NGYYYYIRYEEGKEYPINCRRKGSMNAPEEIILDENEVAAGHRYCQIASIDISPDNHF
                     MAFGVDTLGKRLFTIYFKDLASGRILEHKIENTTGEVAWTKDCRFVFYAFKDSTLRPS
                     AIYRHDILQPYDDVQVYKETDKTFRAHVFTTKSNSFVIISTSNGIASECWYLDAEKPL
                     DKFRLIAGREQNLEYSVDHSGSHFYFLTNLNAPNFRVLRAPVTNPTLKNWKEVIPADS
                     SVLILTMEAFKNHIVTTERRDALLKVRVINLLNMEQHYIDFGEEVYTALPSVNPEFNT
                     NLFYYYYTSFTTPGSTCEYNMTTRKKRLLKQNEVLGGFDPTKYESKRIWATAPDSTTI
                     PISLVYRKGTRFSANTPLLLYGYGAYGYSINTTFDPNLISLLDRGVVYAVAHVRGGQE
                     MGRHWYNQGKLLNKKNSFTDFVACAEKLIADGYTSASKLIAHGSSAGGLLVGAALNMR
                     PDLFKAVVAEVPFVDVLTSMLDSTQALTTVEYNEWGNPHDSTFYYYIKSYSPYDQIKK
                     MPYPNMLVTSGYNDNQVQYFEPVKWVAKLRANNTGNSQILLWIDMSLGHSQSSGRYLQ
                     LKDQAFIYAYMLNQWGITE"
     gene            5131..5634
                     /locus_tag="PLW22_10345"
     CDS             5131..5634
                     /locus_tag="PLW22_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU75142.1"
                     /translation="MWRSFFTVLIVVGLFGCKTNRHTYSYDTIDVVDFKMLEPQLSKS
                     TDTVYVVNFWATWCAPCVKEFPYFDEAAKKFSGSKVRFLMVSLDFPNQLEKSLKPFVK
                     SRTNRLPVVLLDDPNQNEWINKVSPDWSGALPATLIYKGSYKVFIEGPIELADITSNI
                     DSLLVKP"
     gene            5648..6247
                     /locus_tag="PLW22_10350"
     CDS             5648..6247
                     /locus_tag="PLW22_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU75143.1"
                     /translation="MKRLFLMLMATFMVAGLYAQGLKIGDKAPDFRLKNVDGKMVSLA
                     DYTQAKGFIVIFSCNHCPFVKAYEDRMIALHEKYASKGFPVIAINSNDPEVVPEDSFD
                     KMVERAQQKKFPFVYLFDEGQKIYPLYGAARTPHVYILSKKANDLVVEYIGTIDDNYK
                     DASAVKEKYVENAVDALLSGKKPAVTETKAVGCSIKVKK"
     gene            6322..>6830
                     /locus_tag="PLW22_10355"
     CDS             6322..>6830
                     /locus_tag="PLW22_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU75144.1"
                     /translation="MDGLRVVPTADGSSTLYSERFKATYHSVNGALTESMHVFIKSGF
                     EAILLNKKNVDVLEIGMGTCLNAALTGWVAQMHGVKVHYTGIELFPPTGEVLNSTGYH
                     AVLELECAKLWEKIVAASAIEPVPVSTWFTVSKLYVDFIGWNPNHMYDLVYFDAFAPD
                     DQPEMWSIE"
BASE COUNT         2077 a         1301 c         1485 g         1967 t
ORIGIN      
        1 atgcggcaag ttacaaaaaa atagcatacc tgcaattggc tcagcccaat atgcagctct
       61 ggctatgtta ttttttgcaa aacccaaaag gtagttgttg acattttgat taaaccaagg
      121 tgcaatgaat taaaaattca aacgggataa tttttggagg aatgaatcgg aatgaatcgg
      181 aatgagtcgg aatgagtcgg aatgaatcgg aatatgagga agttagagga agttctagtt
      241 cgttaaacgt gaaccgtgaa ccgtgaaccg gaagaattaa gaatatgacg gagttagagg
      301 aagttagagg aaaatagaac agataaatct aagaattttg ataatcaaat ttaacctaac
      361 aaaaaataga acgcaggtta cactgattaa atagaattgc agattttcaa taatcaccca
      421 acaccaaaca ctaaacacta aacactaaac actaaacact aaacacctta tatacaatta
      481 actaaaaaac gttgattcaa atcccaaaaa aagtagctga tttttttatt gttatgtaaa
      541 tttacaatat cttgaagtta aaattgaaag aataattatg gatgaggttg agatcaatgc
      601 aatacttgca gagcttgatg atttaaggaa gaagtacgag gagttaaagg ctcttcatga
      661 tgcccaaaat aagattcaaa gcgatataga aagcagattg cttgatagcg aggcccgtaa
      721 tgctgcactg ttatcggcca accctgatat gatgtttgtt ttcgataagc aaggttattt
      781 tattgactac aatggagggc ttaatgagat gtatgctcct ccggaattct ttgtgggtaa
      841 gagagtagat gaggtgttac cagcaccaat tgcaacgcta acccacgaaa agataaagca
      901 gctttttgaa accggtaaaa tgcaggttta cgactacgac cttgttattc agggtaaaac
      961 aatgtttttt gaaagccgcc ttgttaagta tggcactgat aaagcattag ccgtagtaag
     1021 aaatattacc caaaggaaga actatgaaat taccctaaag gagagtgaac tccgatatca
     1081 ggaactatat aaccttttac gattacttgc cgatacggct cccgatatga tttgggcaaa
     1141 ggatgctcaa aagcgatata tttttgccaa tagggcaatg tgtcaagagc tgctcaatgc
     1201 gtctgacacc aatgaaccag taggtaaaac cgatttatat tttgctcttc gcgaaaggga
     1261 gaaacatcca gaggatccca actggcatac ttttggggag ctatgtatgg attccgatga
     1321 aattacaatg cgcgagctac gccccatgca gtttgatgag tatggtaatg ttaagggcaa
     1381 attcatgttc cttgatgttc ataaggcccc cattttcagt aacgatggaa agttaatggg
     1441 agttgttggt acggcccgta acattaccga aaagaaggag atggaggaaa agttaaaact
     1501 atcagaacaa acatacaaag gaatcctaaa cagtatttcg gaggcagttt acatacagga
     1561 tgaaaacgga gtttttatag atgtaaacga tacagccacc cgaatttacg gttaccctcg
     1621 tgaatacttt ataggcagaa ctcctgaatt cctgtcggcg ccagggtata ataacctgga
     1681 gcaggttgcg cagtgcgtga aaaaggcttt ccatggcgaa cctcaggttt ttgaattctg
     1741 ggggatacga cacgatgggg cctacttccc aaagattgta agccttacgc caggagtata
     1801 ttttggtaaa aattgtgtaa ttgcggtggc acgcgatatt actgaacaaa agaactggga
     1861 aaaagagata ctcgaggcaa agagtaaagc agaggaaaat gataagctaa aatcggcctt
     1921 tctggccaat atgagccatg aaattagaac tccattgaac ggcataatgg ggtttgtgca
     1981 gcttttacgc aacccaaatt tgtccaaaga cgatttggat aaatacctaa atgtaattga
     2041 aagaagtggg gagaggctta atgaactgtt aaccggcctc ctcgatatgg ctagaattga
     2101 ggcaggccaa gtcaaagtaa tttacaaaag gaccaatatt gcaagcctgg tacagtacgt
     2161 aaccgaattt tttacccccg atgcaagggt aaaaggctta ggaataactc ataaaatcaa
     2221 tatccctgag cagttagttg agattgaaac cgatggctac ctgttacagg atatcttttt
     2281 taacctgatt aaaaatgcca taaaatatac aaataaggga aacataacca ttggttgtga
     2341 gtattttgat agttcactac tatttaaggt aagcgataca ggtataggag taccacggga
     2401 taaacaaaaa gtgatttttg atcgctttgt gcgagccaat aatgatttga ctaatccggt
     2461 tgatggtgct ggtttgggat tatcaatagc aaaggcttat gttgaaagac ttggtggtaa
     2521 aatttggttg gaaagcgaac ccggtagagg ctcaaccttc tacttcacta ttcccttaag
     2581 tccaaatgtt aacttctaat tcatcaactg actaaaattc gtcaactgtt tttttcgata
     2641 aacagctccc ctctttaaat gtttccgcct tccattttga ttatctcagc tgaatttatt
     2701 aatccatagc aattcggatt aactgttcac tttttgctat tcctctaact tcctctaact
     2761 ccctcatatt ccgactcatt ccgattcatt ccgtctcatt cctcaagaaa taaatccgtt
     2821 tgaattttac aatttatggc acacgggtta aatagccgtc tccctttgcc agaaatactt
     2881 attcggttat accccactgg tttaacatgt aggcatatat aaaagcctga tccttgagct
     2941 gaaggtacct acccgatgat tggctatggc caagcgacat atcaatccaa agcaaaattt
     3001 ggctgttacc ggtattatta gcccgtagct tagccaccca ctttacgggc tcaaagtatt
     3061 gcacttgatt gtcgttgtaa cccgatgtaa caagcatgtt agggtagggc atctttttaa
     3121 tttggtcgta gggcgagtac gattttatgt agtagtagaa ggtggaatcg tgaggatttc
     3181 cccactcgtt gtattcaaca gtagttaatg cttgtgttga atcgagcatc gatgtaagca
     3241 catctacaaa aggcacctct gcaaccacag ctttgaatag atcggggcgc atgtttaatg
     3301 ctgctcccac aagtaatccg ccagcactgc taccatgagc tataagtttt gaagctgagg
     3361 tatagccatc ggcaataagt ttctcggcgc aagcaacaaa atcggtaaaa gagtttttct
     3421 tgttcaggag tttgccttgg ttgtaccagt gccgacccat ctcctgccca cctcgcacat
     3481 gggcaactgc atatactacc cccctgtcaa gtaagctaat gagatttgga tcgaaggtgg
     3541 tgttgatgga gtaaccgtaa gcaccataac cgtaaaggag caagggtgta tttgcgctaa
     3601 acctggtacc tttcctatat accagtgata ttggaatcgt agtactgtcc ggggcagttg
     3661 cccaaatccg ctttgactca tatttagtag gatcaaaacc acccagaact tcattttgct
     3721 tgagcagcct ttttttacgg gtagtcatgt tgtattcgca agtggaaccg ggagtggtaa
     3781 acgaggtgta gtagtaataa aaaagattgg tgttaaactc agggtttact gaaggaagag
     3841 cagtataaac ctcctcgcca aaatcgatat aatgctgctc catattcagt aggtttatta
     3901 cccttacttt caatagcgca tcgcggcgtt cagttgttac tatgtggttt ttaaaggctt
     3961 ccatggtaag aattagtacc gaggagtcgg cagggattac ctctttccag ttcttaagtg
     4021 ttgggttggt aactggagcc ctaagcaccc tgaaattggg agcgtttagg ttggttaaaa
     4081 agtaaaaatg actacctgag tggtcaacgc tatactcaag attctgttct cgccccgcta
     4141 tcaatctaaa tttatctaag ggcttttcgg cgtccaggta ccagcactca cttgcaattc
     4201 cattggaagt tgatattatt acgaagctgt tcgattttgt ggtaaacaca tgtgccctga
     4261 aagttttgtc ggtttcctta taaacctgga catcatcgta tggttggagt atatcgtggc
     4321 ggtaaatggc tgatgggcgc agggtgctat ccttgaaggc ataaaaaaca aatcggcaat
     4381 ccttagtcca ggcaacctca ccggttgtgt tttcaatttt atgctccaaa atccgacccg
     4441 atgccaaatc cttgaagtat atggtaaaaa gccgtttccc cagggtatca accccaaatg
     4501 ccatgaaatg gttatcgggc gagatatcaa tgctagcaat ctggcagtag cgatgacctg
     4561 cagcaacctc attctcatca agtattattt cttcaggcgc attcatggag ccctttcgcc
     4621 tacagttaat gggatactcc ttgccctcct cgtatcgaat atagtagtag tagccattca
     4681 tgaatacggg tacagtaata tcgtcgcctc gtttgcgcga gcgcatttca gcaaccaacc
     4741 tatcttgcag gcttaaggta ggctccatta ctgcatatgt ataggcattc tcggccagta
     4801 aatggtcaat taccttttgc gattcgcgat tccgtaacca gaagtaagga tcgatgcgtg
     4861 tatccccaaa cataaccaga agcgtatcgt gttgttcggc taaaggtgcg gtgggtacta
     4921 aggaacagcc cgaaattatg agggtagcaa aaaaaagaaa agtatttttt aaggctctta
     4981 tcatctacac caaaatgagt tttaaacatc aaatttatta cttccgttgg tttttaccaa
     5041 attgctgtta ctataaattg aatagcgtgc ataataaacg gcatgtgaac aaagaaacat
     5101 tttgtttgtt aaacagaaaa tattcgggca atgtggcgat cgttttttac agttcttatt
     5161 gtggtaggtc tgtttggttg taagacaaac aggcatactt atagttacga caccattgat
     5221 gttgttgact ttaagatgct tgagccacag ctctcaaaat caaccgatac ggtttacgta
     5281 gttaactttt gggctacttg gtgtgcgcca tgcgttaagg aatttccata ctttgatgaa
     5341 gctgcaaaaa aattctccgg tagcaaggta agattcttaa tggttagcct cgatttcccc
     5401 aatcagcttg agaagagctt aaaaccattt gtaaaatcca gaactaatcg tttacctgta
     5461 gttttgctcg atgatcctaa tcagaatgag tggatcaata aggtgagccc cgactggagc
     5521 ggtgcgcttc ctgcaaccct aatatataaa ggcagctata aagtttttat tgaagggcct
     5581 attgaattag ccgatataac ttcaaatatt gatagtttac ttgtcaaacc ttaaaactaa
     5641 tgaatctatg aaacgcttgt ttttgatgct aatggctact tttatggtgg ccgggctata
     5701 cgctcagggt ttaaaaattg gcgataaggc ccctgacttt cgcctgaaaa atgttgatgg
     5761 caaaatggtt tcactggccg attacactca ggccaaaggt tttattgtaa tcttttcgtg
     5821 taatcattgc ccttttgtga aagcctatga ggataggatg atagccttac acgaaaagta
     5881 tgcctctaaa gggtttcctg taattgccat caactctaac gatcctgagg ttgtacctga
     5941 agactcgttc gataaaatgg ttgagcgagc ccagcaaaag aaattcccat ttgtgtacct
     6001 cttcgatgag ggacaaaaga tttacccgct ttacggagca gctcgtacac cccatgttta
     6061 cattctaagt aaaaaagcaa acgatttggt tgttgagtac atcggtacaa ttgacgataa
     6121 ctataaggat gcctcggccg taaaggaaaa gtacgttgaa aatgctgttg atgctttact
     6181 ttcgggtaag aaacctgccg ttacagaaac taaagcggtg ggttgctcaa taaaagtgaa
     6241 aaagtagttt aaagcaatat tattgattaa gccgctactg agctagcggc ttttttgtta
     6301 gctttgccta aataatctgc tatggatgga ttaagggtag ttccaactgc cgatggtagt
     6361 tccacgcttt acagcgaaag gtttaaggca acctaccatt ccgtaaacgg tgctctaacg
     6421 gaatcgatgc atgttttcat aaaatcgggg tttgaggcca ttttattaaa taaaaaaaac
     6481 gttgatgtgc ttgagattgg aatgggaacc tgcttaaatg ctgctcttac aggatgggta
     6541 gcacaaatgc atggtgttaa agtacattac accggtatag agcttttccc tcctacgggt
     6601 gaagtgctaa actccacggg ttaccatgct gttttagagc ttgaatgcgc aaagctttgg
     6661 gaaaaaattg ttgctgcatc agccattgag cctgttccag tgtctacttg gtttacagtt
     6721 tcaaagttgt atgttgattt cataggttgg aatcctaacc atatgtacga tttagtttac
     6781 tttgatgctt ttgctcctga cgaccagccc gagatgtgga gtattgaaaa
//
LOCUS       DAOOWJ010000127         6829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_11186_length_6829_cov_6.489076, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000127 DAOOWJ010000000
VERSION     DAOOWJ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11186_length_6829_cov_6.489076"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLW22_10360"
     CDS             complement(<1..240)
                     /locus_tag="PLW22_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU75145.1"
                     /translation="MRRIFSKTVITLSLVSYFTDIASEMLYPVMPIYLKSIGFTALLI
                     GILEGVAEATAGLSKGYFGELSDRLGKRVIFIRWGY"
     gene            381..3194
                     /locus_tag="PLW22_10365"
     CDS             381..3194
                     /locus_tag="PLW22_10365"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU75146.1"
                     /translation="MTIKKSIVLTFSMLVLASIQVLGQGWNTPVPVDPNVRYGKLENG
                     LTYYVRKNTEPKQRAEFYIAQNVGAILEEDSQNGLAHFLEHMAFNGTKNFPGKGIINY
                     FETVGVKFGFNINAFTSVDETVYNLSDVPTTREGIIDSALLVLHDWSHFISLLPEEIE
                     SERGVIREEWRTGRNAERRMYKQLMPVMYKGSKYAERDVIGDINVINNFKHQELVDYY
                     NKWYRPDLQSIVVVGDIDVDQVEAKIKSLFADIPAPVNPAPRPFYELPDNDEPLVGVA
                     TDPEARNAIMYLYFKAPATPKEAKNLGYLRTDMVNELISSMISSRLNELVQKPNPPFV
                     FGASGYFNLVRTKDALAFYAALRPDNMIDGVKGLIRESERVKQYGFNATELDRAKSDY
                     LRSLENQLKEKDKQKNEKYVWECVDHFLKGEPMPGIEFEYMFASQLLPTIKIEEINAV
                     AKQLVTDKNVILTLMAPQKDGITVPNQEQLLAALKEVKAENIEAYVDKVITKPLVEKI
                     EKPGKVKKENVKNVYGTTEWILSNGVKVVVKQTDFKEDQVVVDAFSAGGVSLASIDEL
                     PSASMATQIITNAGVGEFTSTDLEKMLAGKMVNVRPVIAESYEGFSGEASPKDFETLL
                     QLIYLYMTAPRIDEENFNTYMGRMKAFMANASSDPRMAFRDSITVALANHNPRVMPMN
                     LDYLNKVDFNKSINFYKNRFADASDFTFIFTGNVSPVEIKPLVEKYLGALPTVKRKDV
                     AKDNGVRPPKGKVQNYFTKPMQTTKSSVFVGFTGEVEYNVMNDILADYLTSILRTRYL
                     EEIREKEGGTYGVGVRVSLRKFPVNSFVVQIQFDTDPKLRDKLIGIVYSEIEKIKTNG
                     PLADDLNKSKEFMLKEYEQNQRENSYWTNVIRAKYEDGLDFDSKYVEMVKAVDVNAIK
                     EFANKIFGQGNVVEVVMTSEEQK"
     gene            3289..4413
                     /locus_tag="PLW22_10370"
     CDS             3289..4413
                     /locus_tag="PLW22_10370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU75147.1"
                     /translation="MRPLLSLVLTIICSIGFAQYDSTLRLIFIGDVMGHVPQIQSAYD
                     SSTGRYDYTDVFSELKGLLTSADYSIANLEVTLGGEPFTGYPQFSSPDELVDGLMAAG
                     VNVLVTANNHSVDRGGDGIRRTIDVLAAKNLLFTGTFLDSTDRSIRNPIVLNKNGVKL
                     ALLNYTYGTNGIPAPSPYLVNLIDTVSMANDIARAQALGVDDIVVFIHWGNEYERLPN
                     MQQKRLTGWLHGKGVRIIIGSHPHVVQPAEFNRFENDFRLVVYSLGNFVSNQRRRYCD
                     GGLVVLVDLMKQNGKLEVSGVGYIPVWVDTYFSSGKRKYKVFPVGMYEGKSNQFLSAA
                     SDSAFKVFVDDTRSLLNANNINFPEVKLVNGEWVVPLLSR"
     gene            complement(4403..>4652)
                     /locus_tag="PLW22_10375"
     CDS             complement(4403..>4652)
                     /locus_tag="PLW22_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOU75148.1"
                     /translation="RISDISESHGCPLARILRKRLHRLGIRQGVKVVYSPEEVPENAT
                     RPCEGEPNKKTTIGTISYMPPLFGCMIASVVVRDLLND"
     assembly_gap    4653..4752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4753..4955)
                     /locus_tag="PLW22_10380"
     CDS             complement(<4753..4955)
                     /locus_tag="PLW22_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOU75149.1"
                     /translation="MVPTWLERTELLLGDKAVEKLSQKHVLIIGLGGVGAYAAEQICR
                     AGIGALTIADGDTIHESNINRQLP"
     gene            complement(5011..5691)
                     /locus_tag="PLW22_10385"
     CDS             complement(5011..5691)
                     /locus_tag="PLW22_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU75150.1"
                     /translation="MKLVNFHTHKPQFNSNSICVVPYDVDSYNLYSSSHYKTIGLHPW
                     KTESSEISHWLGKLNELAKQPDFIGIGEIGLDRLKGAPLKNQIDIFEKILETAKEFKK
                     PIIIHCVRCWTELIAILSKSKYSELPKAIHGFRAKADVAKQLVDNGFYLSFGTALIHA
                     TPELAEALTLVPRNKLFLETDDTEIPIYEVYDAAADILDSTIEELVSVTNQNLLTFFG
                     NTLSDKSK"
     gene            complement(5723..5995)
                     /locus_tag="PLW22_10390"
     CDS             complement(5723..5995)
                     /locus_tag="PLW22_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOU75151.1"
                     /translation="MKTIAITVKGKVQGVGFRYFVMRHANEIGVNGFVKNLPNGDVYI
                     EASGTNSQLEQLILLCNQGPAHSNVENVNVKDIQQSFDIIGFQILG"
     gene            6050..6541
                     /locus_tag="PLW22_10395"
     CDS             6050..6541
                     /locus_tag="PLW22_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOU75152.1"
                     /translation="MILDYFLLVFSITLILLGLAGCILPVIPGPPLAWLGLLLAKFTS
                     FCSVDWITIMVMAGVTLIVTLLDYLFPAWIATKMGGSNWGGWGTIIGLLVGVVFLGPL
                     GAVVGPFVGALVGEMLRKQPQNNTKNNPWLSAVGSFLGFMLGVGVKLITVGVIIYFLV
                     VSF"
     gene            complement(6545..6829)
                     /locus_tag="PLW22_10400"
     CDS             complement(6545..6829)
                     /locus_tag="PLW22_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU75153.1"
                     /translation="MGNIAYTMTADELKELCSPFGNVVSAKIITDRATGKSKGYGFVE
                     FDNEESAQKAIKELNDTQVKGRNIKVNSAFRKTDQPQASKGKEEEPDTKS"
BASE COUNT         1885 a         1342 c         1478 g         2024 t
ORIGIN      
        1 ataaccccac ctaatgaaaa tcactcgttt ccccaacctg tccgatagct caccaaagta
       61 acccttgctt aatccggcgg ttgcttcggc aacgccctca agaattccta ttagcaatgc
      121 agtaaatcct atcgatttta agtagatagg cattacagga taaagcatct cacttgcaat
      181 atcggtgaaa tagctaacca gggagagggt gatgacagtc tttgaaaaaa tgcgtctcat
      241 aaattaaact ttatacttaa tttgatgtca tagggttaca aattactgtt aaaaccttta
      301 agttttcttt taggttttta ataaatgtgt aattttgggt tgaattctag aaacaaacct
      361 acattaaatt ttaaaaagtt atgacaatta aaaaaagtat cgtattaacg ttttcaatgc
      421 ttgttttagc aagtattcag gttcttggtc aaggatggaa tactcctgta ccggttgatc
      481 caaacgtacg ctatggtaag ttggaaaatg gtcttactta ctatgtacgc aaaaacacag
      541 aacccaagca gcgtgctgag ttctacattg cccaaaacgt aggagcaatc ctggaggagg
      601 atagccaaaa cggattagct cacttcctgg agcatatggc tttcaatgga actaagaact
      661 tcccgggcaa gggtatcatc aactactttg agaccgttgg ggtgaaattt ggttttaaca
      721 tcaatgcttt cacctcagta gatgaaacag tttacaacct atcggatgtt ccaactaccc
      781 gcgagggtat tattgatagc gctttactgg tacttcatga ttggtcgcac tttatttcgc
      841 ttcttcccga ggagattgaa tcagagcgag gtgtaatccg cgaggagtgg cgtacaggcc
      901 gtaacgctga gcgcagaatg tataaacagc tcatgcctgt tatgtacaag ggatctaagt
      961 acgctgagcg tgatgtaata ggcgatatca atgttatcaa caactttaag caccaggaac
     1021 tggttgacta ctacaataaa tggtatcgtc cggatttaca atcgattgta gtggttggtg
     1081 atattgatgt tgatcaggtt gaggccaaga ttaaatcgtt atttgctgat attccagctc
     1141 cggttaatcc tgcgccccgt cctttctatg aacttcccga caacgatgag ccccttgtag
     1201 gtgttgctac cgatccggag gctcgcaatg ccatcatgta cctatacttt aaggcaccgg
     1261 caacacctaa agaggcaaaa aacttgggtt atctccgtac cgatatggta aatgagctca
     1321 tctcgtcgat gatttcatca cgattaaacg aacttgttca gaaacctaat cctccttttg
     1381 tgtttggtgc atcgggttac ttcaatctgg ttagaaccaa ggatgctctt gcattttatg
     1441 cagctcttcg tcccgataac atgattgatg gtgttaaggg acttattcgt gagtcggaac
     1501 gcgtaaaaca atatggattc aatgctacag agctcgatag ggcaaaatcc gattacttac
     1561 gctcacttga gaatcaactc aaggaaaagg acaaacaaaa gaacgagaag tacgtttggg
     1621 aatgtgttga tcatttcctg aaaggcgaac ccatgccagg catcgaattt gaatacatgt
     1681 ttgcctcaca gctattgcct acaattaaga ttgaagaaat aaatgcggtt gccaaacagc
     1741 ttgttaccga taaaaatgtc attctaaccc ttatggctcc ccaaaaggat ggcatcactg
     1801 ttccaaatca ggaacaattg cttgctgccc taaaggaagt aaaggccgaa aacattgagg
     1861 catacgttga taaggtaata accaagccat tggttgaaaa gattgaaaag cccggtaagg
     1921 ttaaaaagga aaacgtaaag aatgtatacg gtaccactga gtggattctc tccaatggag
     1981 taaaggttgt tgtgaagcaa accgatttca aggaggacca ggttgttgtt gatgctttca
     2041 gtgcaggtgg cgtttcgctt gcatccatcg atgagctacc ctcggctagt atggccaccc
     2101 aaattattac caatgccggt gtgggcgaat ttaccagcac tgaccttgaa aagatgcttg
     2161 ctggcaagat ggttaatgta cgtccggtta ttgccgagag ctacgaaggt tttagcggtg
     2221 aagcatcgcc taaggatttt gaaaccttac tacagctcat ctacttgtac atgacagcac
     2281 cacgtatcga tgaggaaaac ttcaatacct atatgggtag aatgaaggct ttcatggcca
     2341 atgcctcgtc cgatccacgc atggccttcc gcgatagcat taccgttgcc ctggcaaacc
     2401 acaaccccag ggttatgcca atgaaccttg attacctgaa caaggttgac tttaacaaga
     2461 gcatcaactt ctataagaat cgctttgccg atgcttccga tttcactttt atctttaccg
     2521 gaaatgttag cccagttgag ataaagcctt tggttgaaaa atacctgggc gcattgccca
     2581 ccgttaagcg taaagatgtt gccaaggata atggagtaag acctcctaaa ggcaaggttc
     2641 agaattactt tacaaagcca atgcaaacca ccaagtcgtc agtatttgtt gggtttaccg
     2701 gtgaagttga gtataacgtg atgaacgata tacttgccga ctacctcaca tccatattac
     2761 gcacccgtta tcttgaggaa attcgtgaaa aagaaggtgg tacttatggt gttggagtta
     2821 gggtatcgct ccgcaaattc cctgtaaact cctttgtggt tcagatccag ttcgataccg
     2881 atcctaagct tcgcgataaa ctaattggta ttgtttactc tgagattgaa aagattaaga
     2941 ccaatggacc attggccgat gatctgaaca agagcaagga gttcatgctc aaggagtacg
     3001 agcaaaacca gcgcgaaaac tcttactgga caaatgtgat tagggcaaaa tacgaggatg
     3061 gccttgattt tgactcaaag tatgtggaga tggtaaaggc tgttgatgtg aatgccatta
     3121 aggaatttgc caataagatt tttggacagg gtaatgttgt tgaggtagta atgacctccg
     3181 aagaacagaa atagcattag gtttttcaat acaaaggggt atccgtaatg ggtacccctt
     3241 ttttgttgat aggaaagttt ttcctatcat tgtacaaaca tcatagttat gagacctcta
     3301 ctatccctgg tgctaactat tatatgcagt attggttttg ctcaatacga tagcacccta
     3361 cgcttaattt ttattggtga tgtaatgggc catgttcctc aaatccaatc ggcatacgat
     3421 agttcaaccg ggcgatacga ttataccgat gttttttctg aattaaaagg cttgctcact
     3481 agtgccgatt attccattgc caaccttgag gtaactttag gaggagaacc ctttactggt
     3541 taccctcagt ttagctcacc cgatgaactt gtcgatggtt taatggcggc tggtgttaat
     3601 gttttggtta ccgccaataa ccattcagtt gataggggag gggatggtat aaggagaacc
     3661 attgatgttc ttgcggccaa aaacttactt tttacaggga cattcctcga ttcaactgac
     3721 aggtcaattc gaaatcctat tgttctaaat aaaaatgggg taaagcttgc cttgcttaac
     3781 tatacctatg gaaccaatgg aattccagcc ccaagcccat acttggtcaa tttgattgat
     3841 acagttagca tggcaaatga tattgctaga gcacaagctc taggagtgga cgatattgtt
     3901 gtgttcattc actggggcaa tgagtacgaa cgtttaccta acatgcagca gaaacgatta
     3961 actggttggc tgcatggtaa aggggttcgt attattattg gttcgcaccc ccatgttgtt
     4021 cagcccgctg agtttaaccg tttcgagaac gattttagac tggttgttta ctcgcttggc
     4081 aactttgttt ccaaccagcg tcgaaggtac tgcgatggag gtttagttgt tcttgttgat
     4141 ttaatgaagc aaaatggaaa gctagaagta agtggtgtgg gatacattcc ggtttgggtt
     4201 gatacatact tttcaagtgg aaagcgtaag tataaggttt tcccggtggg gatgtatgag
     4261 ggcaaaagca atcagttcct ttcagcagca agcgactccg ctttcaaggt ttttgttgat
     4321 gatactcgta gtttgctgaa cgccaacaat ataaatttcc ccgaagtaaa gctagtaaat
     4381 ggcgaatggg tggtgccttt gcttagtcgt taagcaaatc cctaacaacc actgaggcaa
     4441 tcatgcaacc aaagagtggc ggcatgtacg aaatggttcc aatggtagtt ttcttattgg
     4501 gttcaccctc gcagggtctt gtggcattct cgggaacttc ctcgggcgaa taaacaacct
     4561 taaccccctg ccgtattccc aagcggtgta gtcgttttct taggattcta gcaagtgggc
     4621 atccatgcga ttcggaaata tcggaaatcc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnggcaattg gcgattaatg tttgattcgt ggatagtatc gccatcagca
     4801 atggttagtg caccaattcc tgcacggcag atttgttcgg cagcgtatgc gcctactccg
     4861 ccaagcccaa taattagtac atgcttttgc gatagctttt caacggcttt gtcgccaagt
     4921 aatagttcag ttcgttcaag ccaggtggga accatatcat gttttttgct cagcaaaggt
     4981 cgtaatcagt atcattaaat ccaataacct ttacttgctt ttatcggaaa gggtgttccc
     5041 aaaaaatgtt aacaggtttt ggtttgtaac actaacaagt tcctctatgg tggagtcaag
     5101 aatatcggcg gctgcgtcgt aaacctcata aatgggtatc tcggtatcgt cagtttcaag
     5161 gaatagttta tttcttggaa caagcgttaa agcctcagct aactctggtg tggcatgtat
     5221 aagcgcggtt ccgaatgata ggtagaatcc attgtcaaca agctgtttgg ctacatcggc
     5281 tttggctcta aagccatgta tggctttagg tagttcacta tattttgatt ttgatagtat
     5341 tgctatcagc tcagtccagc atctaacaca gtgaattatt attggctttt taaattcttt
     5401 ggcagtttca agaatttttt caaaaatatc gatttgattc tttaaaggag cgccttttag
     5461 cctatctaac cctatttccc ctattccaat aaaatcaggc tgcttagcca gctcatttag
     5521 cttgccaagc caatggctaa tctcactgct ttcggttttc caggggtgta aaccaattgt
     5581 tttgtagtgc gatgaactat aaaggttata actatcaaca tcgtaaggca caacacaaat
     5641 gctattgctg ttgaattgag gtttatgtgt atgaaagtta accagcttca ttgttaaatt
     5701 ctttatgtgg ttttgaacta agttaaccaa gtatttggaa tcctataata tcaaatgact
     5761 gttgaatatc tttaacattg acgttttcta catttgaatg tgcaggtccc tggttgcaca
     5821 gtaaaatcag ttgctcaagc tggctgtttg ttccgcttgc ttcaatgtaa acatcgccat
     5881 tgggcaagtt cttcacaaaa ccattaacac caatttcatt ggcatggcgc attacaaagt
     5941 atctaaaacc tacaccctga acctttccct ttaccgttat tgcaatggtt ttcattctag
     6001 gaaaaaatga tatatttacc aaagttattg aataagaaca aaaaagcaaa tgatactaga
     6061 ttactttttg cttgtatttt ccattacgct aatactgctg ggactggcag ggtgtatttt
     6121 acctgtaatt ccagggccgc cgctagcgtg gctggggctg ttgctggcaa agtttacatc
     6181 gttttgctct gtggactgga tcaccattat ggttatggca ggtgtaactc taattgtaac
     6241 cctgctcgat tacttgttcc ctgcctggat agccacaaaa atgggtggaa gtaattgggg
     6301 tggctggggt acaatcattg gcctacttgt tggggtagta tttttagggc cacttggagc
     6361 cgttgttgga ccttttgtag gagcgcttgt tggcgagatg ctaaggaaac aaccccaaaa
     6421 taataccaaa aataatccct ggctttcggc agtaggatca tttcttggat ttatgttagg
     6481 agtaggtgta aagctaatta cagtaggtgt aataatctac tttttagtag ttagctttta
     6541 atctttagga tttagtatcg ggctcctcct ctttaccctt gcttgcttgg ggttgatcgg
     6601 tttttcggaa tgcgctatta accttaatat tccgaccctt aacctgagtg tcgttaagtt
     6661 cttttatggc tttctgagcc gattcctcat tatcaaactc aacaaaaccg tagccttttg
     6721 attttccggt agcacgatcg gtaatgattt ttgctgaaac cacatttcca aaaggcgagc
     6781 aaagttcctt taattcatcg gcggtcattg tatacgcgat gtttccaat
//
LOCUS       DAOOWJ010000128         6775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_11311_length_6775_cov_5.561607, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000128 DAOOWJ010000000
VERSION     DAOOWJ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11311_length_6775_cov_5.561607"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="PLW22_10405"
     CDS             complement(<1..1454)
                     /locus_tag="PLW22_10405"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOU75154.1"
                     /translation="MATLERIRNRAGVLVSVVIGLALFAFILGDFITSGGVFFNRSQM
                     EIAEIAGSSISYEEYQARVSENENLQKLFSQQSSLNEQQMLQIREQVWQDLLRANILQ
                     PEYKRLGLVIHEDELFDMVQGRNIHPFISQQFGDPQTGEVNKAFLTNFLQNLDRDPRA
                     KAYWLFIENEIQKDRLFNKYLTLVRKGLGVTSLQAKRAVNDRTSKVNFDYAVASYQSI
                     SDSLVNVSNSDIKAYYKSHQNEYKQTASRDIEYVVFPILPSEADVNDAEAWIQKALPD
                     FIGASDPAQYVTLNSDSKFDSKYYKASELPENVRAWAFSKPVGSLLEVYRDGDSFKAS
                     RLVDVKMMPDSVKARHILIGLRSQSAEEREKAKAKADSIMNVLKRGGSWEQLAAKHSD
                     DPGSKDKGGDLGWFPSGMMVPEFDKASFSSNKGEIKVVETQFGFHVLQVVDRGKEEKK
                     VQIATLERKIVPSSYTTSQVYNKAAAFAGNNRDY"
     gene            complement(1573..>1995)
                     /locus_tag="PLW22_10410"
     CDS             complement(1573..>1995)
                     /locus_tag="PLW22_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOU75155.1"
                     /translation="DFFPETMKANELLTHFIKAHKSMAIVVDEFGGTSGLVTIEDIIE
                     EIFGEIDDEHDLNELVEKKLSDKEYILSGRVEVSHVNEAYGLGIPESDEYETIAGFIL
                     HHYQSLPKPGDSLKIQNFEFKILRVNKTRIDLVRLVID"
     assembly_gap    1996..2095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2096..2288
                     /locus_tag="PLW22_10415"
     CDS             <2096..2288
                     /locus_tag="PLW22_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75156.1"
                     /translation="SKRNLFDDRKAISTPEKKAENKIATAITSSCVPIFYYWFSIFFR
                     LLLRKKYISKATAAITKNM"
     gene            complement(2482..3054)
                     /gene="lptC"
                     /locus_tag="PLW22_10420"
     CDS             complement(2482..3054)
                     /gene="lptC"
                     /locus_tag="PLW22_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOU75157.1"
                     /translation="MIRLNIKVFKNMVVLTLVGATSLFFVSCKGDSEAIVYLSDIKTT
                     PGVTAYNSEVVYTEGGKVVLKVIAPETVYYQFADEPYTEFPKGITVYTYDNELKQESS
                     LTANYAIYYEKKLLWQAKNNVVAKNRKGEQLYTEELYWDQNKRIIYTNVNVKINSVSG
                     IIYGKGMIADENFDNWEVKEPYDGEFEVDQ"
     gene            complement(3057..4394)
                     /locus_tag="PLW22_10425"
     CDS             complement(3057..4394)
                     /locus_tag="PLW22_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75158.1"
                     /translation="MKFRNYIRLFAGVLLVSVSTSSIAQSYLENPKYGPDEESRKECA
                     KNLSLYGTFYQQSNYKDAYKPWQNVIRICPAASINTYIRGVKLLKARYEEVADPAQKK
                     VLVDSLMMIYDLRITHFDKKGMVLGQKAEDLFTLAPDRYEEAFNIAKEAISIEKANSN
                     PSTLFAYVSIAKAMYDNKKLTADQVIELYSQVSDYIDAQVTANPSDDKAKQAKDGIDA
                     IFAGMGVANCDNVIALFEPRFKATPEDVNLCKKIRSLMSANRCTSHPLYLNASIQIFK
                     GEPNADLALDIARLEYEAKNAREAEKYYNEAINLESVVEKRANIYYELALLTFTELKN
                     LPKAREIALKAIEENPNMGKAYKLIGDIYASERNCGSNDFEKKTVYWVAVDKYIKAKQ
                     VDPELTDAMDNLISTYSQYYPSKEDIFFYDLKVGDSYTVGCWINERTTVRDRK"
     gene            complement(4424..5725)
                     /locus_tag="PLW22_10430"
     CDS             complement(4424..5725)
                     /locus_tag="PLW22_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75159.1"
                     /translation="MYRIAFRRFLILAGLLSLSEFAGAQNLNTYSPYSRYGIGVLSQP
                     GFTQNRSMGGVSQPYRSETVINFNNPASYAQRDSMSFLFDFGIETNTSGFTGYDQYLN
                     PQTSRNSAGGIHHIAISFPITKRIGFAAGLTPYSFVGYRLLRFETDSYILSTIGRIKY
                     YHTGRGGINQAFVGMGVSPVKNLAVGFNMLYYFGSLDYNNDIEFPLSYNPYTNGYIRS
                     SFQVSDINFNFGVQYSLVLDKENNTNITLGGTYQLGKNINMTQKWFATLESNLLDTLY
                     PHPDRQSKLELPWSVNAGLAFTYKDKLTIMGEYFTQDWSKTTPFNVNTPMAKMESVRF
                     GVEFTPNRMDLKSYFKKVNYRAGFFTNKSNLLVSGKQINDFGITFGAGLPLKGKTKVN
                     LSFEIGKRGTDESYLIKETFKAFNFSFTFYDYPWFFKRKYN"
     gene            complement(5718..6437)
                     /locus_tag="PLW22_10435"
     CDS             complement(5718..6437)
                     /locus_tag="PLW22_10435"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOU75160.1"
                     /translation="MNLIIDIGNTLSKVAIADGKEVRFFQSFHELDIDDLMPIINDFK
                     PERCIVSAVGKIPVNTLVWLRQNLPVHIAGANTNIPLKNEYSTPETLGFDRLALAVGA
                     NFLFPNQNVLVIDAGTAITYDLVTSEGVYKGGAISPGLRLRFKALNDYTAKLPLIEHF
                     KIVDIVGTSTVECISSGVINGTVLEIEGYVGKINSIYEGCAVVLTGGDANFLANMLKN
                     SIFVNPFLMVIGLNRILEFNV"
     gene            complement(6454..>6775)
                     /locus_tag="PLW22_10440"
     CDS             complement(6454..>6775)
                     /locus_tag="PLW22_10440"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75161.1"
                     /translation="LRDRFGPIPDPVIELMSIVRLRWIAVELGIEKIILKNGKAHAYF
                     ITNQLSPFYRTSTFERIINYILRNPGIFSMKEGNEKLILTIKDVKGVGKAIDILQQML
                     DNVR"
BASE COUNT         1921 a         1463 c         1240 g         2051 t
ORIGIN      
        1 tcgtaatcac ggttattccc tgcaaaagct gcagctttat tgtaaacctg cgatgttgta
       61 taagagctag gaacaatttt gcgttcaagg gtagcaatct gtactttctt ttcctccttg
      121 cccctatcaa caacctgaag tacatggaag ccaaactggg tttctacaac cttaatctca
      181 cccttgttgc tgctaaacga tgccttatcg aactcgggaa ccatcatacc actggggaac
      241 caacctaaat caccgccctt gtcctttgaa ccgggatcgt cggaatgttt agctgctaac
      301 tgctcccaac taccaccgcg ttttaaaacg ttcataatac tgtcggcctt ggctttagct
      361 ttttcgcgct cctcagcgga ttgacttctc aaacctataa gtatgtggcg agcctttacg
      421 ctgtcgggca tcattttaac atcaaccaac ctcgaagcct tgaagctatc gccatcgcga
      481 taaacctcca acaaacttcc aactggtttt gaaaaagccc atgcccgaac attctcaggt
      541 aattccgaag ccttataata cttagaatcg aattttgagt cggaattcag ggtaacatac
      601 tgagcgggat cggaagcgcc aataaaatca ggcaaagcct tttggatcca tgcttctgca
      661 tcgttcacat cggcttccga tggaagaatg gggaacacaa cgtactcaat atcgcgcgaa
      721 gctgtttgct tgtattcgtt ctggtgcgac ttgtagtatg cctttatatc gctattggaa
      781 acattaacca aagaatcaga aattgattgg tacgaagcaa cagcatagtc aaagttaacc
      841 ttgctggttc ggtcgtttac agcacgtttg gcttgaagcg aggtaacgcc taatccttta
      901 cggactaaag taaggtattt gttgaataat ctatctttct gtatttcatt ctcaatgaat
      961 aaccagtagg ctttagccct tgggtctcta tccaaattct gaaggaagtt tgtaagaaac
     1021 gctttgttca cttcgccggt ttgagggtcg ccaaactgtt gactgataaa tgggtgtatg
     1081 ttccttccct gaaccatgtc aaaaagttcg tcctcatgaa tcacaagccc taaacgctta
     1141 tattcgggtt ggagaatatt agccctaagc agatcctgcc aaacctgttc acggatttga
     1201 agcatttgtt gctcgttaag cgatgattgt tgcgagaaaa gcttctgtaa attctcattt
     1261 tcgctaaccc tagcctggta ttcctcgtag gatatagaac taccggctat ctcggcaatt
     1321 tccatttgtg agcgattaaa gaacactcct cccgatgtaa taaaatcgcc aagtataaag
     1381 gcaaaaagag ccaatccaat tactacagaa accaatacgc ctgcacggtt tctaattctt
     1441 tcaagtgttg ccatctacaa gtatatttta aggtacaata atatttttcg agccacgaat
     1501 atagtaattt tcaacaataa gcagtttaac tgcctgataa ttttttaaca accgttaaaa
     1561 accagtagta acctagtcaa taaccaaccg aaccaggtcg attcgcgttt tattcaccct
     1621 tagaatttta aactcaaagt tttggatttt taacgaatcg ccaggttttg gtaaactctg
     1681 gtagtggtga agaatgaatc cggctatagt ctcatactcg tccgattcag gaatgccaag
     1741 cccataagcc tcattaacat gcgatacctc aacgcgccct gaaaggatgt actccttatc
     1801 ggaaagcttt ttctcaacca gctcgtttaa atcgtgttca tcgtcaattt caccaaaaat
     1861 ttcttcaatg atatcctcaa tggtaaccaa tcccgaggtc cctccaaact catctaccac
     1921 aatagccata cttttatggg ctttaatgaa gtgggttagt agttcattgg ccttcatggt
     1981 ttcagggaaa aaatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcaa
     2101 agcgtaactt atttgatgat agaaaagcta tctcaacacc cgaaaagaaa gccgaaaaca
     2161 aaatagcaac cgctattacc agtagctgtg ttcccatctt ttactattgg ttctccattt
     2221 tctttcgcct acttcttcgc aaaaaataca ttagtaaagc aactgcagcc attacaaaaa
     2281 acatgtagct gttgcttaat cctttgtagg tagcccaaat tcccataccc aaacaaaata
     2341 gggcaagggc aagccagatt aactctagga taaatgccaa cttattcatg taaaaaagta
     2401 tgtatggttt acaataggta aaggtacaaa aaaaggattg tatgtcaata caacccttaa
     2461 atgctttaaa cgatttaatt attactggtc aacctcaaac tcgccatcgt agggctcttt
     2521 aacttcccag ttatcaaaat tttcatcggc aatcattccc tttccatata taatgccgct
     2581 aacgctatta attttaacat taacgttagt ataaatgatg cgtttattct gatcccagta
     2641 aagttcttcc gtgtaaagct gttcgccctt cctgttttta gccactacgt tgttctttgc
     2701 ctgccaaagc aatttctttt cgtagtaaat ggcatagttt gctgtaaggc tcgactcctg
     2761 ctttaactca ttatcgtaag tgtaaactgt tatcccttta ggaaactcgg tgtagggctc
     2821 atcggcaaac tggtagtaaa ccgtttcagg ggctattact tttaaaacca ctttaccccc
     2881 ctcagtgtaa actacctctg aattataggc tgtaacaccg ggtgtagttt ttatatcgga
     2941 aaggtaaact atagcctcag aatcgccctt acaagaaacg aaaaataaag aagtagcccc
     3001 caccagggtg aggactacca tattcttaaa tacttttatg tttagcctaa tcatttttac
     3061 tttctatccc ttacagttgt acgctcatta atccagcatc ctactgtgta tgaatcgcca
     3121 acctttaaat cgtagaagaa gatgtcctct tttgaggggt agtattggct gtaagtgctt
     3181 ataaggttat ccatcgcatc ggtaagttca ggatcaacct gctttgcctt aatgtattta
     3241 tccactgcaa cccagtaaac agtcttcttt tcaaaatcgt tgctgccaca gttgcgctcg
     3301 ctggcatata tatcgccaat cagtttatag gctttaccca tgttgggatt ttcctcaatg
     3361 gcctttaatg caatttctct ggctttgggc aaattcttaa gttcagtgaa agtgagcagt
     3421 gcaagttcat agtaaatgtt ggctctcttt tctacaaccg attcaaggtt aattgcctca
     3481 ttgtagtact tttctgcctc ccgggcattc tttgcttcgt actccagcct ggcaatatca
     3541 agggctaaat cagcattagg ctcaccctta aaaatttgta tgctggcatt taagtatagt
     3601 gggtggctag tgcacctatt tgccgacatc agcgagcgaa tttttttgca aaggtttaca
     3661 tcctctggag tagccttaaa acggggctca aaaagagcaa taacgttatc gcagttagct
     3721 acacccatgc ctgcaaaaat agcatcaatt ccatccttag cctgttttgc tttatcatcg
     3781 ctggggttcg ctgtcacctg agcatcaata tagtcgctca cctgtgagta aagttcaatt
     3841 acctgatcgg cagtcaactt tttgttgtcg tacatagcct ttgcaattga aacataggca
     3901 aagagtgttg atgggtttga attagccttt tcaatgctaa tagcctcctt ggcaatgtta
     3961 aaagcctcct cgtaacggtc cggtgcaagg gtaaataaat cttcagcctt ctggccaaga
     4021 accataccct ttttatcaaa atgggtaata cggaggtcgt aaatcatcat cagtgagtca
     4081 actaagactt tcttctgggc aggatcagca acctcctcgt aacgagcctt taaaagcttt
     4141 acgcctctaa tgtatgtgtt aatactagct gcgggacaaa tcctaataac attctgccaa
     4201 ggtttatagg catccttata gttgctttgc tggtagaatg tcccgtaaag cgataggttt
     4261 ttagcacatt ccttccggga ttcctcatcg ggcccatact taggattttc caagtacgac
     4321 tgagcgatac ttgaagtgct tacagaaaca agtaacacac ctgcaaataa cctaatgtaa
     4381 tttctgaatt tcatggctta tggttttatt tcgatattaa acattaattg tatttccgtt
     4441 taaagaacca tgggtaatcg taaaaagtaa aactaaaatt aaacgcctta aaggtttctt
     4501 ttattaagta gctttcatcg gtgcctctct tcccaatttc aaatgatagg ttaaccttgg
     4561 ttttcccctt taacggtaaa cctgctccaa atgttatgcc aaagtcattt atttgttttc
     4621 cacttactag taggttcgat ttgttggtaa aaaaacctgc tctgtagtta acctttttga
     4681 aatagctctt taaatccatc ctgttgggag tgaactcaac accaaaccgt actgactcca
     4741 tttttgccat gggggtgttt acgttgaaag gggtggtttt tgaccagtcc tgagtaaagt
     4801 attcacccat aatagtaagc ttatccttat aagtaaatgc taaaccagca ttcacgctcc
     4861 agggtaattc aagtttcgat tgcctatcgg gatgaggata aagtgtgtcg agtaggttac
     4921 tttcaagggt tgcaaaccat ttctgggtca tatttatgtt tttcccaagc tggtaggttc
     4981 cccctagtgt aatatttgta ttgttttcct tatcgagaac aagcgaatac tgtactccaa
     5041 agttaaagtt gatatcgctt acctgaaagc tggatcgaat atacccgttg gtgtaaggat
     5101 tgtagctaag agggaattct atgtcgttat tataatcaag gctaccaaag tagtataaca
     5161 tgttaaaccc aaccgctaag tttttaaccg gacttacccc cattccaaca aatgcttggt
     5221 taatccctcc tctgccggta tggtagtact ttatccttcc aatggtactc aggatgtagg
     5281 aatcagtctc gaaacggagt aacctgtacc ctacaaagct gtatggtgtt aaacccgcag
     5341 caaatcctat ccgtttggtt atagggaacg atatagcaat atgatgaata ccacctgcac
     5401 tattacgcga tgtttgtgga ttaagatact ggtcgtaacc tgtaaaaccg ctcgtgttgg
     5461 tctctatacc aaaatcaaac aggaacgaca tgctatccct ttgggcgtag gatgcagggt
     5521 tgttgaaatt aataacggtc tcgctcctat agggttgcga tacacctccc attgaacggt
     5581 tttgggtaaa ccctggctga ctcaaaactc caataccata ccgtgagtat ggcgaataag
     5641 tgttgaggtt ctgtgctcct gcaaattctg aaaggcttaa cagccctgcc aaaatcaaaa
     5701 aacgtcgaaa tgctattcta tacattgaat tcaagtattc tgtttaaacc tattaccatt
     5761 aaaaatgggt tcacaaatat gctatttttt aacatattag ccaagaaatt tgcatcgcca
     5821 cctgttaaaa ccacagcaca accctcataa attgagttaa tttttccgac ataaccctca
     5881 atttccagaa cagttccatt aattactccg gaactaatac attcaaccgt tgatgttcca
     5941 acaatatcaa caattttaaa gtgctcaatt aacggtaact tagcagtata gtcgttaagt
     6001 gccttaaagc gtaaacgtaa cccaggcgat atagcgcctc ccttgtatac cccttcactg
     6061 gtaaccaaat cgtaggttat ggctgttcca gcatcaatta ctaaaacgtt ctggttcggg
     6121 aagaggaagt ttgccccaac cgctagcgca agcctgtcga aacccaaggt ttcgggggta
     6181 gagtattcgt tctttagggg aatattagta tttgcaccgg caatatgtac aggcaagttt
     6241 tgccttaacc ataccaaggt gtttacgggt attttaccaa cagccgaaac aatgcatctc
     6301 tcgggcttaa aatcattgat aattggcatt aaatcgtcaa tatcaagctc atgaaagctc
     6361 tggaagaatc ttacttcctt tccatcggct atggcaacct tgcttaaagt atttcctata
     6421 tcaataatta ggttcataat ttgctttaaa ttactaacgg acattatcga gcatttgttg
     6481 tagaatatca atagccttac ctactccttt tacgtcctta atggttaaaa tgagcttttc
     6541 gttaccctct ttcatggaaa aaattcctgg gttacgcaaa atatagttaa tgattcgttc
     6601 aaaagtagat gttctgtaaa acggtgatag ctgattggta ataaagtagg cgtgagcttt
     6661 accgtttttg agaataatct tttctattcc caattctacg gctatccatc gtaacctcac
     6721 aatgctcatc aattcaataa caggatcggg aattgggcca aacctatcgc gaagc
//
LOCUS       DAOOWJ010000129         6256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_12603_length_6256_cov_7.844864, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000129 DAOOWJ010000000
VERSION     DAOOWJ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12603_length_6256_cov_7.844864"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      53..143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            235..2688
                     /locus_tag="PLW22_10445"
     CDS             235..2688
                     /locus_tag="PLW22_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU75162.1"
                     /translation="MKKIAFVLCALLFAGFTMQANPTENLGFTIYGQITDTYGTPLVG
                     ATVKLENTFLGTYSGYNGYYRLTQVRQGSYTLVASYLGYETVQVEVKVNGDMELNLEL
                     KQSSQYLDAVVVSSTRASSRMPIAQTTLNRQELKQANTGGDTPYLLEMLPSVVVSSES
                     GIGIGNTAFRIRGTDPTRINVTINGVPLNDAESQGVYWVDLPDFAASVDNVQVQRGVG
                     TSTNGAGAFGATVNFQTTTLTTEPYAAVDLLGGSYNTWKTSARIATGLMKNGFSMEGR
                     FSQLHSDGYIERANSDHRSMFVTAAWNGERSLVRANIIHGEEHTGITWEGTPDYMMST
                     NRRYNPAGEYVDDQGVTRYYDDQKDNYWQTHYHLMGTHSITNNLTANITLHLTDGRGY
                     YEEYKPDRKLSKYGLPNFSIRDTIIKRTDFIQQKWMDNIFYGGIATMLYRSGIFEYTF
                     GGGWNRYDGDHFGKVLWSKMNIGLPNKYEWYRNNGEKTDWNIFSKAIWQVSDAISVFA
                     DIQYRGISYKLKGIDSDVIALNQEHSWNFLNPKVGATYSFNSGHEAYISYAIAHREPA
                     RSDLKDAQKGSTDFTPKPERLNDWEIGYRMRLQNLSVDFNYYYMLYKDQLVLTGELTD
                     VGYARMANVPNSYRTGIELTLSAKPASWFKIDGNTTLSKNIIKDYTNFANLTDNPTDW
                     NDLQSPVAEPLGNTTISFSPSLVAFTRITILPVENFQFSWVSKYVSRQYIDNTENRNR
                     SLDPYWVNNFITEYSYKTKSNKSIFAQISVNNFFNIKYSANAWVYRTLFQSGDPEYVS
                     IGYYPQAERHIMVRVGIEF"
     gene            2867..3460
                     /gene="pnuC"
                     /locus_tag="PLW22_10450"
     CDS             2867..3460
                     /gene="pnuC"
                     /locus_tag="PLW22_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOU75163.1"
                     /translation="MNWLQNNYIELIGAITGLIYLYLEIKQRVWLWPLGIITSAFYIY
                     IFYESKFYADMGLQFYYLFISIYGWYHWLFGGAVKAKNSLPVTNTPKSNYLSLLAASV
                     AFFVIIRHILVHYTDSPVPTGDAFTTALSITATWMLARKYIEHWWLWVIVNSVSLALY
                     LYKGLYPTSVLFVFYTTMSVVGYYQWKQERSKTIAQP"
     gene            3496..4110
                     /locus_tag="PLW22_10455"
     CDS             3496..4110
                     /locus_tag="PLW22_10455"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOU75164.1"
                     /translation="MKAVILANGEFPSHPKPLNELLNAPLLVCCDGAIDRLENLAITP
                     NALVGDLDSVKEHLKRKYESILYYDPDQNTNDLTKAVKWCLNHRIKNIVIVGATGKRE
                     DHTLGNIGLLCNYARMGVNVTMLTDTGMLLPLLASTRIDSYAGQQVSIFSPNNQTVIY
                     TKNLKYPIVNQTLPELWMGTLNESLGDWFELTFSPGPVIIYKKY"
     gene            4238..5314
                     /locus_tag="PLW22_10460"
     CDS             4238..5314
                     /locus_tag="PLW22_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOU75165.1"
                     /translation="MKHLNSVITGTGSYVPTVRVSNREFARHTFYNKDETAIETLVDE
                     VISKFKDITGIEERRWVTDDLCASHIGAIAAEQAITAAGIDRETIDQIIVAHNFGDVM
                     HGTIQTDILPSLASRIKHHLGIKNPACIPYDILFGCPGWVQGIIQADSYIKSGLAKRC
                     LVIGAETLSRVIDKHDRDCMIFADGAGATIVEALEEDEKRGVISTAMMSHTVNEAFYL
                     YLGKSYHPEADPTIRYIKMEGRKIYEYSLVNVPVAMKVAMDKSGIDPKELKKVMIHQA
                     NEKMDEAIVQRFIRQCKIDKTTTELMPMMIKELGNSSVATVPTLYDLILRGELPEHRI
                     GRGDVIMFASVGAGMNINAIVYKQ"
BASE COUNT         1836 a         1458 c         1368 g         1594 t
ORIGIN      
        1 tatcttttca ccgctcgcgg ttcacttcac gctttttttt actatctttg cagctgactt
       61 ttgggggtgc ccttagggct gagatcatac cctttgaacc tgatccgggt aatgccggcg
      121 aagggaaaaa gagtttgtca acatctcaca atttccccct ttatagttat tcaggatttg
      181 cggcttaaat gtccgaacct ggtttatgtt taactattta aagggataaa aaacatgaag
      241 aaaattgcat ttgtgctctg tgcacttctg tttgcaggtt ttaccatgca agcaaatcct
      301 accgagaatc tcggatttac catttacgga cagattaccg acacctacgg cactccgtta
      361 gtgggtgcta ccgttaagct ggaaaacacc tttttgggta cctacagcgg ttacaatggg
      421 tactatcggc ttacgcaggt gcggcagggt agctataccc ttgttgcctc gtacctgggg
      481 tacgaaaccg ttcaggttga ggttaaggtt aatggcgata tggagttgaa tcttgagcta
      541 aagcaatcgt cgcagtacct cgatgcagtt gtagtatcgt caacccgagc atcgagccgc
      601 atgcccattg cacaaaccac gctcaaccgc caggagctta agcaggccaa caccggaggc
      661 gacacgccct acctgctgga aatgttacca tcggtggtag tatcatcgga aagcggcatt
      721 ggaattggca acacagcctt ccgcattcgc ggaaccgatc caacccgcat taatgtaacc
      781 attaacgggg ttccgctcaa cgatgctgaa tcgcaggggg tttactgggt ggatttaccc
      841 gattttgcag catcggtcga taatgtacag gtgcagcgcg gggttggaac atcaaccaac
      901 ggcgccggtg cctttggcgc aaccgtaaac tttcaaacca caacccttac tactgagcca
      961 tatgcagccg ttgacctact tggcgggtcg tacaatacat ggaaaacatc ggcacggatt
     1021 gctaccggcc tgatgaagaa cgggttcagc atggaaggac ggttctcgca gttacactcc
     1081 gacgggtata tcgaacgagc caactcggat cacaggtcga tgtttgttac tgcagcctgg
     1141 aatggcgagc gcagcctagt tcgtgcaaat atcattcatg gtgaggagca taccggaatt
     1201 acatgggagg gcacacccga ctacatgatg agcactaacc gccgctacaa tcccgcaggc
     1261 gagtatgttg atgatcaagg ggtaacccgc tactacgacg atcagaaaga taattactgg
     1321 caaacccact accaccttat gggtacccac tcaatcacaa acaacctaac cgcaaatatc
     1381 acccttcacc taaccgatgg cagaggttac tacgaggagt ataaacccga tcgtaagctc
     1441 tcaaaatacg gattgccaaa cttttcaatt cgcgatacca tcattaaacg taccgacttt
     1501 attcagcaga aatggatgga caacatcttt tacgggggta tagccacaat gctttaccgc
     1561 tcagggatat ttgagtatac attcggcgga ggctggaacc gttacgatgg cgaccatttt
     1621 ggaaaagttc tttggagtaa aatgaatatc ggcttaccca ataaatatga gtggtatagg
     1681 aacaatggcg aaaaaaccga ctggaatata ttctcaaagg ctatttggca ggtatccgat
     1741 gccatttcgg tttttgccga cattcagtac aggggtatta gctataagct aaagggaatt
     1801 gactccgatg taattgcatt gaaccaggaa catagctgga atttcctgaa tcccaaggta
     1861 ggggcaacct atagctttaa ctcagggcac gaggcttaca tctcgtatgc aattgctcac
     1921 cgcgagccag cccgcagcga cttaaaggat gcccaaaagg gctcaaccga ttttactcct
     1981 aagcccgaaa ggctgaacga ctgggaaatt ggctaccgca tgcgcttaca aaacctttcg
     2041 gttgatttca actactacta catgctttac aaggatcagc ttgtacttac tggcgagctc
     2101 accgatgttg gttatgcccg aatggctaat gttcccaaca gttaccgtac cggtattgag
     2161 ttgacccttt ccgctaaacc agccagctgg tttaaaattg acggaaacac aaccctgagc
     2221 aagaacatca tcaaggacta tacaaacttt gccaacctta ccgataatcc aaccgattgg
     2281 aacgacctgc aatcgcctgt agctgaaccc ctaggcaaca caacaatatc gttctcgcct
     2341 tcgctggtag cattcacacg cattacaatt ttacctgtag agaattttca gttttcgtgg
     2401 gtaagcaagt atgttagcag gcagtatatc gataacaccg aaaacagaaa ccgcagcctt
     2461 gatccttact gggttaacaa ctttataaca gagtatagct acaagacaaa atcgaataaa
     2521 tcgatttttg ctcaaatttc ggtgaataac tttttcaata tcaagtactc cgccaacgcc
     2581 tgggtttacc gcacactatt ccagagcggc gatcctgagt atgtaagcat tggttactac
     2641 ccccaagccg aaagacatat tatggttagg gttggcattg agttttaatt ggaaatatga
     2701 ttactgggaa atgcaatcgt taaaagatag ggagttgatt ttggtattca gggtaagaaa
     2761 gcattcaaaa tcctgaactc taagagcaaa ttccctatct ttacctttta ccctttatcc
     2821 tttatccttt agcctttagc ctttatcctt taactttatc cttaaaatga actggctaca
     2881 aaacaactac attgagctaa ttggtgcaat cactggtctc atttacctat acctggagat
     2941 taagcaaagg gtttggcttt ggccgctggg cataataacc tcggcattct acatctacat
     3001 tttttacgag tcgaagtttt atgccgatat gggcctgcag ttctactacc ttttcataag
     3061 tatttatggc tggtaccact ggttgtttgg cggagccgta aaggctaaaa actcattacc
     3121 tgttacaaac actccaaaaa gcaactactt atctttactt gcggcaagtg ttgctttttt
     3181 tgtaataatc cggcacatac ttgttcacta caccgactca ccagttccca caggcgacgc
     3241 gtttaccaca gcgcttagca ttactgcaac ctggatgctt gcccggaaat acattgagca
     3301 ttggtggtta tgggtgatag tcaattccgt atcgcttgcg ctataccttt acaagggact
     3361 ctacccaaca tcggtgctgt ttgtttttta caccaccatg tcggttgtag ggtattacca
     3421 gtggaaacag gagagatcca aaaccattgc tcaaccataa gcacaaatct tactcatttc
     3481 aaaaaaaaca atattatgaa agcagttata ctggccaacg gagaatttcc ctcgcatcca
     3541 aaaccactca atgagctgct aaatgcccca ttgctggtat gctgcgatgg cgccatcgat
     3601 aggcttgaga atcttgccat aacacccaac gccttggtag gcgaccttga ttccgtaaag
     3661 gagcatctca agcgcaagta cgaaagcata ctctactacg accccgacca aaacaccaac
     3721 gaccttacaa aagcagttaa atggtgcttg aaccatagaa taaaaaacat tgtaatagtt
     3781 ggcgcaaccg gaaagcgtga ggatcacacg ttagggaata tcggattgct atgtaactac
     3841 gcccgaatgg gagttaatgt taccatgctt accgatactg gcatgctact acccttactg
     3901 gcatctacca ggattgacag ctatgcaggc caacaggtgt caatcttttc gccaaacaat
     3961 caaactgtaa tatacaccaa gaacctaaaa tatccgattg taaaccaaac tttgccggaa
     4021 ctatggatgg gaacgctgaa cgagagttta ggcgactggt ttgagttaac cttttcaccc
     4081 ggcccagtaa taatttacaa gaaatactaa atttcaattt ataaccattc tatcattcta
     4141 tttatcttta tattaacact ttttactctc aattcatttt gattttagtt acattttatt
     4201 acttttacag cgggactaat caaacgttta ttgtattatg aaacacttaa attccgtaat
     4261 caccggaacg ggtagctatg tgccaaccgt tcgggttagt aaccgtgagt tcgcccgcca
     4321 tacattttac aacaaggacg aaaccgccat cgaaacgctg gtggacgagg taatttcaaa
     4381 gttcaaggac attaccggca ttgaggagcg acgctgggta accgacgatt tatgtgcatc
     4441 gcacataggc gccatagcag ccgaacaggc aattaccgca gccggaatcg accgtgaaac
     4501 cattgaccaa attattgtgg cccacaactt tggcgatgta atgcatggaa ccattcaaac
     4561 cgacatactg ccaagcttgg catctagaat aaagcaccat ttgggtatta aaaacccagc
     4621 gtgtatccca tacgatatac ttttcggctg cccgggatgg gtgcagggca ttattcaggc
     4681 cgattcctac attaaatcgg ggctggccaa acgctgcctg gtaattggag ccgaaaccct
     4741 atcgagggtg attgacaagc acgaccgcga ttgtatgata tttgccgatg gagctggcgc
     4801 caccattgtt gaagcactgg aggaggatga aaaaagaggc gttatatcaa cagcaatgat
     4861 gtcgcatacg gttaatgagg cattttacct ttacctgggc aaatcgtacc acccagaggc
     4921 cgaccctact atacgctaca ttaaaatgga aggccgaaaa atatacgagt actcgcttgt
     4981 taacgtacca gttgccatga aagtggctat ggataaatcg gggattgatc ccaaggaact
     5041 taaaaaggta atgatccacc aggcaaacga aaaaatggac gaagccattg ttcaacgctt
     5101 tatccgccag tgcaagattg acaaaaccac aaccgaactc atgcctatga tgataaagga
     5161 attgggcaac agctcagtgg ccacggttcc caccctttac gacctaatcc tgcgagggga
     5221 actacccgaa cacagaatag ggagaggtga tgtcataatg tttgcatcgg ttggcgcagg
     5281 aatgaatatt aacgcaatag tttacaaaca gtaataagta ttacagccgg gctatttgct
     5341 agaggaatga ggcggaatag gtggaatgtg atggagttag aggaagtttt aatccgttag
     5401 acgtgaaccg tgaaccgtta aacgggaaag gaaaatatgt tatgcccaca gtcattccga
     5461 aggataaagg ataaaggata aaggttaaag gttcttttac gaacaacggt tcacgaacaa
     5521 cgatcaacta atcccctcat cccctctttt ggcgaatcac cttcatgtaa cttgctacca
     5581 gccccgtagg ggctaagcat ttgtaactcc atgaaactcc cacttgtata gcgatgcacg
     5641 ctaggacttt ccaaagagca aaactgaaac gttgcatcgc aatataaaag tattgcaaag
     5701 agcaatttac acgttctttc ttgtcttgat acaagaaaga accaaagaaa atcaaggctg
     5761 aaaagcccaa cccgatgggt gccccgtggg tggaactgcc acgcgataca attcgccacg
     5821 cctgcggcgt gactcatgtg gtatcgctta ctaggcccat cgccacgttc cacccccgac
     5881 ccattctcgg gtcaggcttt tatgccaatg cttttagcat tcctttttgg atgaggaatc
     5941 ctattaatga agagttgaag aaacaaagag atgcttcggc aaagtctacc ccgaaatatc
     6001 gggactcagc atagcaaaga gcgtaattat acaaggggta ttgtcattcc gagctagtcg
     6061 aggaatcttc ttcaagtata ccgcgaccag ccccgtaggg gcgtaatatt tgtaacacct
     6121 agattattct taaatgtata gcgatgcacg caatacatat gccaaagagc aagggtgagg
     6181 ctttgcatcg caaaggaaaa acatggcaaa gagcaattta cacgttcttt cttgtcttga
     6241 tacaagaaag aaccaa
//
LOCUS       DAOOWJ010000130         6156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_12856_length_6156_cov_6.733158, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000130 DAOOWJ010000000
VERSION     DAOOWJ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12856_length_6156_cov_6.733158"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..626
                     /locus_tag="PLW22_10465"
     CDS             45..626
                     /locus_tag="PLW22_10465"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU75166.1"
                     /translation="MKFELKKNKIPSMRINLIHLLVISAMALCQSVYSQEISVGQVVP
                     DITGNGPNGEEFKLSSLRGKLVLVDFWASWCSPCRKENPVLAEVYNKYKDACFTNGNG
                     FAIFSVSLDLKRDNWVNAIADDKLVWPYHISDLKGWRSEAAKAYSVNAIPMNFLVDSS
                     GVVIAKNLRGSELSNTLRKYRKWKFFSNSDCKN"
     gene            645..3716
                     /locus_tag="PLW22_10470"
     CDS             645..3716
                     /locus_tag="PLW22_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOU75167.1"
                     /translation="MKTKRFIVRLLKYFGLTIIFLHLLVFIIIGIAKLFEGSIIRLAV
                     KEIESNLKAPISYGEVSLVPFRDFPNLTVRIADFKLGQQADSVRVFGIGSLPDTLVGF
                     KNVFVSVKAYPLLKDKVIINGVELNGFRVNYLVDSTGKSNFDFLIPPDTVETPVDTVT
                     QTILNILLKNLTLSDIHLTYSDQKLKAAADIVIPKLHLEGRVSGDSIWGKSNGEIVVT
                     NTRFADLPLEKVTNLNFSYSVDYDNGNLLLDYAKFDINGIELKATGNATLSDSIWVDF
                     SLALPKLNLAELYGFVPEGMIKEYGLSKLGGTLKFNTIVRGYLYDTLMLPSVFVDFDL
                     RKGVVETKQYPKVNKLDFEGSVSIPNPNYLATSAVNIKKFNLETQQSRANLTLTLKNP
                     NKPLYNIQGDMHLVLDEFAPLLPDSTLTELSGLIDAKFFTKGKMPEYLDVNSSDYFMD
                     NTGFSLSFSNINAGVDTVNAVKELNAEVTYSPGKELTVNNLSLRLPAYKVSLKDGLLK
                     VKVIGRISELDKIDVRVDTLHIAMGNSSFSSKMKFKNLKSPVFRGEGRLYVDLDELHR
                     FIPDTLVSLVTGKIDFGFSTSGTINPDSIDKQIMPILFENSMFAAQIKDFGFSLPNDS
                     LIGVDRLSLTLSMANDTIRLANVNGHYKNLDFRSDSTSIWNVYKTFILGQKDKKLIVN
                     TSLWANYVDYDIFLPFMAEDSTASPDTAASTIPQYIIRGIAKADKVKYGKVLIDDLST
                     KFRVDDSLYVLDELRMKAFGGNMVTSLVYDTRKYPLEIIEFKNESNRLNIKQLLEQND
                     DFEQDYITHKNIDGLVTGTVFGRIVMQDTNLLYDKMNLLGNFKLENGGIYNFEPAMEL
                     SKFTNLNELNNIVFNTLETSVFIYNNQIYFPKTNIVSTAMDMSVYGMQSFADDYEYHF
                     VVYPGDVMLGKSKKLLKKQGLKDEGFEGPNKAKRSGLYLVAFERGKESKYGFDTKELQ
                     ATMKATIRVQERGLNLVFHPHVMNFSTELDRKESKRKTADEKKDGKEVKTN"
     gene            3679..4332
                     /locus_tag="PLW22_10475"
     CDS             3679..4332
                     /locus_tag="PLW22_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOU75168.1"
                     /translation="MKKRMGRKLRPIEWIAVAISIVLIVLIGIKAWQLAPNKTSKLAA
                     PISSNPLDITFGSDSAQLAIYAYISYHCGYCRLFFTEVYPELEREFISTGKVRLVLKI
                     VEHSNNADRINEVKTAVCINRYGYYGKLHELLVTNSSVIYSDEFREMVNHFIDSDPLV
                     AECILGNESMDYILQIRNEFDKFGFTGTPTFVIEGRVFRGYMPFEQFKSIIVKHLNQ"
     gene            4337..4705
                     /locus_tag="PLW22_10480"
     CDS             4337..4705
                     /locus_tag="PLW22_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75169.1"
                     /translation="MRKKLLVAPIILGAVLAFLTWGCEKEEEDVCQKFEPPQCEIANV
                     CCPTDGGNCYYEVGDSKYYCDKTKATPDDPDGCNDAENQAIEVLCPAASPDQLMNAKV
                     ELSKLTRKLMQEARTLSVCH"
     gene            4752..>6156
                     /locus_tag="PLW22_10485"
     CDS             4752..>6156
                     /locus_tag="PLW22_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOU75170.1"
                     /translation="MLVKTFGSAVHGIKATTITIEVSVGQGVNFFLVGLPDSAVKESQ
                     QRVMSALETNGYKFPKKRIVINMAPADIRKEGSAYDLPLAIGIMAASEQIKPDLLPDY
                     VIMGELSLDGTIQPIKGVLPIAIQAREEGFKGFIVPAQNAREAAVVNNLDVYGVESIK
                     QVVDFFNGNSTLEPTIVNTREEFFAHANNYDVDFSDVKGQENVKRALEVAAAGGHNII
                     MIGPPGAGKTMLAKRIPTIIPPLTLREALETTKIHSVAGKIDRNTSLMTRRPFRSPHH
                     TISDIALVGGGQFPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRVITISRAK
                     FTVEYPASFMLVASMNPCPCGYYNHPEKECVCSPGVVQKYLNRISGPLLDRIDIHVEV
                     IPVPFDKLASAPPAESSAQIRERVVKARQIQEERFVPWADVHCNAQMTSKLIRQFCVL
                     DEAGTAILKTAMERLNLS"
BASE COUNT         1798 a         1239 c         1409 g         1710 t
ORIGIN      
        1 tcttcgtttc atattcaaaa tctctttaaa attccataaa ttgcatgaaa tttgagttga
       61 aaaagaataa aattccaagc atgagaatta acctaataca ccttttggta atcagcgcaa
      121 tggctttatg ccaaagtgtt tattcgcagg agatttctgt tgggcaggta gtccctgata
      181 tcactgggaa tggtcccaat ggagaggagt ttaagctttc gtcgttaagg gggaaactgg
      241 tgctagtcga tttttgggca tcgtggtgct cgccctgtag aaaggaaaac cctgtacttg
      301 ctgaggttta caataagtac aaggatgcct gctttaccaa tggaaatgga tttgcgatat
      361 tcagcgtatc gcttgaccta aaacgtgata actgggtaaa cgcaatagcc gacgataaac
      421 tagtatggcc ttaccatata agcgacctaa aaggttggcg gagcgaggcg gcaaaagcgt
      481 acagtgtcaa tgcaattcct atgaactttc tggtggacag ttcgggcgta gttattgcaa
      541 agaacctccg aggttctgag ctcagcaata cattgcgtaa gtaccggaaa tggaagtttt
      601 ttagtaactc cgattgcaaa aactaataat tccgataaca gggtatgaag acaaagcgtt
      661 ttattgtaag gttgttaaag tattttggcc taactattat ctttttacac ttactggtat
      721 tcattattat tgggattgca aagctgtttg agggaagcat tattcgtctt gcggttaagg
      781 agattgaatc aaatctaaaa gcgcctatct cctacgggga ggtttcgtta gttccttttc
      841 gggatttccc gaatcttact gttcgtattg ccgactttaa actaggtcag caggccgact
      901 cggtgcgggt atttggtata gggagtttac ctgataccct ggttgggttt aaaaacgttt
      961 ttgtttccgt aaaggcatat ccactactca aagacaaggt aataattaac ggggttgagc
     1021 tgaatggatt tagggtaaac tacctggttg attctactgg aaaatcgaat tttgattttt
     1081 tgatacctcc cgatacagtt gaaactcctg tagatacagt tacacaaacc attctaaaca
     1141 ttcttttaaa gaatcttacc cttagcgata ttcatttgac atattccgac caaaagctaa
     1201 aggctgcagc ggatattgtt attcctaaac ttcaccttga gggaagggtt tcgggcgata
     1261 gtatctgggg gaaaagtaat ggcgaaattg ttgtaaccaa tacccggttt gccgatttac
     1321 cactggagaa ggttacaaac ctcaactttt cctacagtgt tgattacgac aatggaaacc
     1381 ttttactcga ctatgccaag ttcgatataa atggcataga acttaaagcc actggtaacg
     1441 caaccctgtc ggattcaatc tgggtggatt ttagccttgc tttgccaaaa cttaaccttg
     1501 ctgagctata tggctttgtt cctgagggta tgattaagga gtatggatta tcgaagcttg
     1561 gaggaaccct taagtttaac acaattgtta gggggtattt atacgatacc ctaatgcttc
     1621 caagcgtttt tgttgatttt gacttgagga aaggtgtagt tgaaactaaa caatacccaa
     1681 aagtaaataa gcttgacttc gagggaagcg tttcaatccc aaacccgaac taccttgcca
     1741 catcagcagt aaatattaaa aaattcaatc ttgaaactca gcaaagtagg gctaacctaa
     1801 ctcttacgct gaaaaatcca aacaaaccac tttacaacat tcaaggcgac atgcatcttg
     1861 tcctcgacga gtttgcccca ttgctacccg atagcactct gacagaactt tccggtttaa
     1921 ttgatgctaa attttttacc aagggtaaaa tgcccgaata ccttgatgtg aacagctccg
     1981 attactttat ggacaacacg ggattttcct tatcttttag caatatcaat gccggggtag
     2041 acacggttaa tgctgtaaag gaattaaatg ccgaggtaac atatagccct ggcaaggaac
     2101 taacggttaa caacctctca ttacgtttgc ctgcctacaa ggtaagttta aaggatggtt
     2161 tacttaaagt aaaggtcata gggcgtatat cggaacttga taaaattgat gtaagggtcg
     2221 atacgcttca tattgccatg ggcaatagta gttttagcag taagatgaag ttcaaaaact
     2281 tgaaaagccc cgttttcagg ggcgaaggcc gtttgtatgt cgatttggat gagctacacc
     2341 gatttattcc tgatacgctt gttagccttg ttaccggtaa gatagatttt ggatttagca
     2401 catcgggaac aattaacccc gattctattg ataagcagat aatgccaatc ctatttgaaa
     2461 acagcatgtt tgctgcgcaa atcaaggatt ttggtttctc attgcccaac gatagcctga
     2521 ttggcgtcga taggttatcg cttacccttt caatggcaaa cgataccatt cgtttagcca
     2581 atgtgaatgg acactacaaa aatctggact ttcgctccga ttcaacctct atctggaatg
     2641 tttataaaac attcatttta gggcaaaagg ataaaaagct cattgttaat acatcgcttt
     2701 gggctaacta tgttgattac gatattttcc taccctttat ggccgaggac tcaacggcaa
     2761 gccccgatac ggctgcctca actatcccac aatacattat taggggcatt gccaaagccg
     2821 ataaggtgaa atacgggaaa gtactaattg acgacctttc gacaaagttc agggttgacg
     2881 atagcctcta tgtgcttgac gaactacgaa tgaaagcatt tgggggcaat atggttacct
     2941 cgttggttta cgacacccgt aagtatccac tggaaatcat tgaattcaag aatgaatcga
     3001 ataggttaaa tatcaagcag ttgttagaac aaaacgacga ttttgagcag gattatatca
     3061 cccataaaaa tatcgatgga ttagttacag gtacggtttt tgggcgaatt gtgatgcagg
     3121 acaccaacct gctttatgac aagatgaacc ttttaggcaa ctttaagctt gaaaatggtg
     3181 gtatatacaa ctttgagcct gccatggagc tgtcaaagtt tactaacctt aatgagttaa
     3241 acaatattgt atttaataca ctggaaacca gtgtgtttat ttacaacaac cagatttact
     3301 tcccaaaaac caacatcgta agtaccgcca tggatatgtc ggtttacggt atgcaaagct
     3361 ttgccgatga ctacgagtat cactttgtgg tttaccccgg tgatgtaatg cttggaaaat
     3421 ccaaaaagct tttgaaaaag cagggtttga aggacgaggg atttgaaggg ccaaacaagg
     3481 ctaaacgctc agggctatac cttgtagcgt ttgaaagagg aaaagaatcg aaatatgggt
     3541 ttgatactaa ggagttacag gcaaccatga aggccaccat tcgcgttcag gaacgcgggc
     3601 ttaatttggt ttttcacccc catgtaatga actttagtac cgaattggac agaaaggaat
     3661 ccaaacggaa aacagctgat gaaaaaaagg atgggaagga agttaagacc aattgagtgg
     3721 attgcagtag cgatttccat tgtacttata gttttaattg gcataaaagc gtggcagttg
     3781 gccccaaata agactagtaa gctagccgca cctatttcat ccaatccttt agatatcacg
     3841 tttggaagcg attcggctca gcttgccatt tacgcataca taagttacca ttgcggttac
     3901 tgcaggcttt tttttactga ggtttatcct gagcttgaaa gggaattcat ttccaccgga
     3961 aaggtacgct tggttctgaa aatagtggag cactcaaata acgctgaccg gataaatgag
     4021 gtgaaaacag cagtatgtat aaaccgatac gggtattatg ggaagttgca tgagctgcta
     4081 gttacaaata gtagcgtgat atatagtgac gagttccgtg aaatggtcaa ccatttcatt
     4141 gatagcgacc cactggttgc cgagtgcatt ctagggaatg aatcgatgga ttatattttg
     4201 caaatccgga acgaatttga taaatttgga tttaccggaa cacctacatt tgttattgag
     4261 ggtagagttt ttaggggtta tatgcccttt gagcagttta aatctatcat agttaaacac
     4321 ttaaaccaat aaagttatgc gaaagaagtt actggttgcc ccaattattc taggagcggt
     4381 actggcattc ctaacctggg gttgcgaaaa ggaagaagag gatgtttgcc aaaaatttga
     4441 gccaccccag tgtgaaatag ccaacgtttg ttgcccaacc gatggcggga actgttacta
     4501 cgaggtaggc gatagcaaat attactgcga taagacaaaa gctactccag atgaccccga
     4561 tgggtgtaat gatgccgaaa atcaagccat tgaggttctt tgtccagctg caagtcctga
     4621 ccagctaatg aatgcaaagg ttgagctaag caagttaacc cgcaagctga tgcaagaggc
     4681 aagaacctta tcggtatgcc attaatgaaa ttaccggaat aggccatact attctggttt
     4741 tttatcgagt tatgcttgta aagacctttg gtagtgcggt tcatggcata aaagccacaa
     4801 caattaccat tgaggtaagt gttgggcaag gggtaaactt tttccttgtt gggttacccg
     4861 atagcgctgt taaggagagc caacagcgtg ttatgtcggc actagagact aatggctaca
     4921 agtttcccaa aaagagaatc gtaatcaaca tggctccggc cgacatccgc aaggagggct
     4981 cggcttacga tttaccactg gccattggaa ttatggctgc atctgaacag attaaacccg
     5041 atttgctgcc ggattacgtt atcatgggtg agctttcgct cgatggcacc atccagccca
     5101 ttaaaggggt tttgcccata gccattcagg cccgcgagga gggttttaaa ggttttattg
     5161 ttcccgcgca gaatgcccgt gaggctgccg ttgtaaacaa cctcgacgtt tacggggttg
     5221 agagtatcaa acaggtggtt gactttttta atggcaatag cactcttgag ccaaccattg
     5281 tgaacacccg cgaggagttt tttgcccacg ccaacaacta cgatgttgat ttttccgatg
     5341 taaaggggca ggaaaacgtt aagcgggcgc ttgaggtggc tgctgcagga ggacacaaca
     5401 tcattatgat aggcccgcct ggtgcaggaa aaaccatgct ggccaagcgg ataccaacaa
     5461 ttattccccc gttaacattg cgcgaggcgc ttgagaccac caaaattcat tccgttgcag
     5521 gtaaaattga caggaataca tcattgatga cccgtaggcc attccgtagt ccacaccata
     5581 ccatctccga cattgctctt gttggtggtg ggcagtttcc acagcccggt gaaatatcgc
     5641 tcgcccataa cggcgtactt tttctcgatg agctacccga gtttaaaaga acagttttag
     5701 aggttatgcg ccaacccctt gaggacaggg ttataaccat atcgcgggca aagtttacgg
     5761 ttgagtatcc cgccagcttc atgttagttg cctccatgaa tccctgccca tgcggttact
     5821 acaatcatcc tgaaaaggaa tgcgtgtgtt cgcccggagt tgtgcaaaag tacctaaatc
     5881 gcatttcagg accgctgctc gaccgcattg atatccatgt tgaggtaatc ccggtcccat
     5941 tcgataagct tgcgtcggca ccaccagccg agagtagcgc tcaaattagg gagcgggtgg
     6001 taaaagcccg acagattcag gaggagcgtt ttgttccctg ggccgatgta cactgtaacg
     6061 ctcaaatgac ctccaagctg atacggcagt tctgcgtact tgatgaggcc ggaacggcca
     6121 tcctgaaaac agctatggaa cggctaaacc tttcag
//
LOCUS       DAOOWJ010000131         6141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_12894_length_6141_cov_9.638022, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000131 DAOOWJ010000000
VERSION     DAOOWJ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12894_length_6141_cov_9.638022"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLW22_10490"
     CDS             <1..339
                     /locus_tag="PLW22_10490"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOU75171.1"
                     /translation="MGEFNFYSINPTSSRKANLSPYVNAGVGLAQMGTSLIFHIPFGV
                     GLKFTPGQRHTFALEWRFHKTFTDKIDDYSSPNDGRKPFLHNNDWFSFIGLIYTYRIP
                     NNSYICPAYR"
     gene            344..1081
                     /locus_tag="PLW22_10495"
     CDS             344..1081
                     /locus_tag="PLW22_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOU75172.1"
                     /translation="MSLIDKIDRSRVPEHVAIIMDGNGRWAKKRGNQRIYGHKNGVKA
                     VKAATEAAGEIGVKYLTLFAFSTENWNRPKSEVDALMSLLVETINSETDTLLKNGIRV
                     LTIGDQSMLPPDVQRKLNDVITRTANCNTITTILALNYGSRHEITEAVKKIAAQTKDG
                     TISPNQITPEIISKYLYTANIPDPALLIRTSGELRLSNFLLWQLAYTELYFTPVLWPD
                     FTKEDFFEAIVDYQKRERRFGKTSEQL"
     gene            1234..3714
                     /gene="bamA"
                     /locus_tag="PLW22_10500"
     CDS             1234..3714
                     /gene="bamA"
                     /locus_tag="PLW22_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOU75173.1"
                     /translation="MMRFRLLALAMFFIGVSAFAQEKPFSYELPKRYCVKEITVSGVK
                     FLNPDVLISMTAISIGDTIQVPGEAITKAVKKLWGQGLFSNVDIKASRIEGDDIYLEI
                     SLTEQPRLTTVNFTGIRKGEADDLKDKLKLRSGSQLTQATLENSIIIIKKHYRAKGFL
                     NVDVNPVLENDTIISNGTKLTFNIQKNDRVKIGEITFEGNKAYGQARLRRALKKIHRR
                     DLNIFKSAKFIEADYDESLDNLVAFYNEHGYRDFKILGDSIYTINPKRIGIHFKIFEG
                     PQYHIRKIEWVGNTKLPAEALTAVLGMKKGDVYDRSLLEKRLFTDENSISTIYMDDGY
                     LFFHLEPVEVQIQNDSVDLEMRIYEGDQATVNRVIIAGNTKTNEHVIRREIWSKPGYL
                     FSKSEITRTLRELGQMGHFDPEKLGIDPLPNQADGTVDLKYSVEEKANDQLEVSGGWG
                     NKMFVGTIGIRFSNFSVRRIFDKDAWRPVPSGDSQSLAIRASTNGTYYKAFSLSFTEP
                     WLGGRKPTNFSFSLYRTIQSGGVSYFYQSSDKYFRVSGASVGIGTRLKWPDDYFTIYN
                     EVGIQNYKLSNWSGYFIFTDGQSNNLSYKVTLGRNSTDQMIYPRTGSNFSLSLQITPP
                     YSLFNGKDYSKPMTDSEKYKWIEYHKWTGRIQWYIPLVENLVLYTNFQMGVMGYFNSK
                     LGHSPFEGFDLGGDGMSGYNLYGKETIGLRGYQNGSLTPYVRIGESLVGNAHLYDKYT
                     VELRYPISLKPQAAIYLLTFLEAGNAWQNAGDFNPFNVHRSAGVGARFFLPMLGMLGV
                     DWGYGFDKVPGRPDAHKGQFHFIIGMPF"
     gene            3788..4297
                     /locus_tag="PLW22_10505"
     CDS             3788..4297
                     /locus_tag="PLW22_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU75174.1"
                     /translation="MKRTILILAALTIGLGAWAQKFAYVDTEYILKKIPSYKAAQEQL
                     DKLSEQYQKEIEEKYAEVDKMYKDYQTEKVLLTEEMKKKREDEIIAQERQVKELQMKY
                     FGRDGLLFKKREELVKPIQDQVFNAIKEIAVEGGYAVIFDAAASPNMIYTNPRFDKSE
                     EVLQRLGFK"
     gene            4334..4852
                     /locus_tag="PLW22_10510"
     CDS             4334..4852
                     /locus_tag="PLW22_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU75175.1"
                     /translation="MRKILGLAIVALLTFSFSGVNAQNYKFGHINSQELLKAMPDRDS
                     AEAKIRKYAESLEDQIEQLQVEFNKKYQDYLQKRATFTDAIREMKEKELTDMQQRAQE
                     YQQVAEQDYQRYQVETMKPVIDKADEAIKKVAKANGFTYIFDTSTGVLLYFSEQSIDI
                     TPLVKKELGITK"
     gene            4926..5744
                     /gene="murI"
                     /locus_tag="PLW22_10515"
     CDS             4926..5744
                     /gene="murI"
                     /locus_tag="PLW22_10515"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOU75176.1"
                     /translation="MSGNCNPIGIFDSGAGGLTVLSELVKVMPNEKYIYFADSANCPY
                     GSKPAEEIINLTDKITRFLTTKGCKVIVVACNTATAAAIDYLRNSYSIPFIGMEPAVK
                     PASQNTKTKSIGVLATAGTFKGRLYIETSRKYAADVNVCYQVGEGLVELVESGKADSP
                     EAEQLLHKYIDPMVDCNIDHLVLGCTHYPFLKPVLERMLPNHVTIVDPAPAVALQTKR
                     VAENSGLLCNQNLVDSTSIDFYSSSNTEVLKILVEMNIRPQYRIEDLYFNNVKL"
     gene            5780..>6141
                     /locus_tag="PLW22_10520"
     CDS             5780..>6141
                     /locus_tag="PLW22_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOU75177.1"
                     /translation="MRKEVEIVIENTGQKVRVTPGHSLLEISKIFEIKSDYQILGAMV
                     NNQVKELDYEVFTPKTVRYINITHPAGMRMYQRSLFFLLQKAVRDIIPGAKVRIEHSV
                     SKGFYCEIDGCNLPLELPL"
BASE COUNT         1918 a         1332 c         1312 g         1579 t
ORIGIN      
        1 atgggcgaat ttaactttta ctccataaac ccaaccagca gccgcaaagc aaacctctcg
       61 ccatatgtaa atgctggggt tgggcttgca caaatgggta cctcgttaat ttttcacata
      121 ccttttggcg taggtttaaa atttacccct ggtcaaaggc acacctttgc cctggaatgg
      181 aggtttcaca aaacctttac cgataaaatt gacgattaca gcagccccaa cgatggccgc
      241 aaaccttttt tacacaataa cgattggttc tcatttattg gattgattta tacctatcgt
      301 attccaaata acagctatat ttgccccgca tatagataga gtcatgtcgc taattgataa
      361 aattgacagg agcagagtcc ctgagcacgt tgccattatt atggacggga atggtcgatg
      421 ggctaagaag cgtggcaacc agcgaattta tggacataag aacggtgtaa aagcggttaa
      481 ggctgccact gaggccgcag gcgaaattgg ggttaagtat cttacacttt ttgccttctc
      541 aaccgaaaac tggaaccgcc ccaagtcgga agtagatgcc ttgatgagtc ttttggttga
      601 aactatcaac tccgagacgg atacactcct gaaaaatggg attcgcgtac tgaccatagg
      661 cgatcagtcc atgctaccgc ctgatgtaca gcgtaaactt aacgatgtta taacacgaac
      721 tgctaactgc aataccataa ctaccattct ggcccttaac tatggctcgc gccatgagat
      781 tactgaagcc gtaaaaaaaa ttgctgctca aaccaaggat ggaacaatat cgcccaacca
      841 aataacccct gaaattattt ctaaatacct atatactgca aacattcctg atccagccct
      901 gctgatacga acaagcggtg agctgaggct cagcaatttc ctgctttggc aattggccta
      961 tactgagctt tactttaccc cagttctttg gcccgatttc accaaagagg atttttttga
     1021 agcaatagtt gattaccaga aacgtgaacg tcggtttgga aaaaccagtg agcaacttta
     1081 aacgttcgta atttttaaca gtagttattg gagattggct atataaaatc atatttttga
     1141 tgccgaaggt tataatactt tcggcatttt gctttgtaac ataggaaatt atactaattt
     1201 tacgcgtcaa aataaaagag taactaattt gatatgatga gatttaggct tttggcactt
     1261 gccatgttct ttataggggt ttcagcattt gcccaggaaa aaccgtttag ctacgaactt
     1321 ccaaaaagat attgcgttaa ggagatcact gtatcggggg taaagtttct aaatcctgat
     1381 gttttaatct ccatgaccgc tattagtatt ggcgacacca ttcaggttcc tggtgaagca
     1441 attaccaaag cagttaaaaa actgtggggc caaggacttt tttcaaatgt ggatattaaa
     1501 gcctctcgta tcgaaggcga tgatatttac cttgagatca gcctaactga gcaaccccgc
     1561 ctaactacag taaattttac tggcattcgc aaaggtgagg ccgacgacct gaaagataaa
     1621 cttaaactcc gaagcggctc ccagctaact caagccaccc tagaaaatag cattatcata
     1681 ataaagaaac attacagggc aaaagggttc ctgaacgttg atgttaatcc cgtactggaa
     1741 aatgacacca taataagcaa cggaacaaag ctaaccttca atatccagaa gaacgacagg
     1801 gttaaaattg gtgaaatcac ctttgagggt aacaaagcct acggtcaggc acgcctacgc
     1861 cgggcactta aaaagataca ccgccgcgac ctcaacatct ttaaatcggc taagttcatt
     1921 gaagcagact acgatgaaag tttggataac ctcgttgcct tttacaacga gcacggctac
     1981 cgggatttta aaattctagg cgattccatt tacaccatca accccaagag aattggtatc
     2041 cacttcaaaa ttttcgaagg gccacaatac catatccgca agattgagtg ggttggcaat
     2101 accaaattgc ctgccgaggc gctcacagcc gttctgggga tgaaaaaggg cgatgtttat
     2161 gataggagtt tacttgaaaa acgtctcttt accgatgaaa actcaatctc aaccatttac
     2221 atggacgatg gatacctttt cttccatctt gagccggtag aggttcagat tcaaaacgac
     2281 tcggttgacc ttgaaatgag aatttatgaa ggcgaccagg ctaccgtaaa cagggtaatt
     2341 attgccggaa atacaaagac caatgagcat gttatccgcc gtgagatatg gtcaaaaccc
     2401 ggataccttt tcagcaagtc cgaaataacc cgaaccctta gggaacttgg ccagatgggt
     2461 cactttgacc ccgaaaaact agggattgat cccctgccca accaggccga tggaaccgtg
     2521 gaccttaaat attcggttga agaaaaggcc aacgaccagc tcgaagtatc gggggggtgg
     2581 ggtaacaaga tgtttgtggg aaccattggt atccgtttct caaacttctc ggttcggaga
     2641 attttcgata aagatgcctg gcgtcccgtt ccatcgggcg atagccagtc attagcaatc
     2701 agagcatcaa ccaacggaac atactataag gcatttagcc tttcgtttac tgaaccctgg
     2761 ctaggcggac gtaagccaac taacttctcc ttctcgctct acagaaccat acaatcaggt
     2821 ggagttagct acttctatca atcgagcgat aaatacttta gggtgagcgg ggcctcagtt
     2881 ggaataggca cccgtttgaa atggcccgac gattacttta ccatttacaa tgaggtggga
     2941 attcaaaact ataagctgag caactggtca ggctacttta tctttaccga tgggcagtcg
     3001 aacaacctta gttacaaagt tacccttggc cgaaactcaa ctgaccagat gatatacccc
     3061 cgaaccggct caaacttttc gctctcgctt caaattaccc caccctactc ccttttcaat
     3121 ggcaaggatt acagtaaacc catgaccgac tcggaaaagt acaaatggat tgaataccac
     3181 aagtggactg gaagaataca atggtatata ccactggttg agaacttggt tctttacacc
     3241 aatttccaga tgggggtaat gggatacttt aatagcaaac taggccattc gccatttgag
     3301 ggttttgacc ttggcggtga tggtatgtcg gggtacaacc tttacggaaa ggaaaccatt
     3361 ggtttaaggg gttaccagaa cggctcactt acaccatacg ttcgcatagg tgagagccta
     3421 gttgggaatg cacacctata cgataagtac accgttgagc tgcggtaccc aatctcactg
     3481 aagccgcagg ctgcaattta cctgcttacc ttccttgaag ctggtaatgc ctggcagaac
     3541 gctggcgatt ttaacccatt caacgttcat cgctcagctg gtgttggggc tcgctttttc
     3601 cttcccatgt taggaatgtt aggcgttgac tggggatatg gtttcgataa ggtgcccgga
     3661 cgtcccgatg cacataaggg tcaattccac ttcattattg ggatgccttt ttaatcctta
     3721 cggttaaaac tgttaaactt ggcatataaa ttgtgtaact ttaacctgaa ccaaatatca
     3781 aaaagttatg aagagaacca ttctgatatt agctgcactt acaattggac ttggggcttg
     3841 ggctcaaaaa tttgcctacg tcgataccga gtacatactt aaaaaaatac catcgtataa
     3901 agccgcacag gaacaactcg acaagctttc agagcaatac cagaaagaaa ttgaggaaaa
     3961 gtatgccgag gttgacaaga tgtacaagga ttaccaaacc gaaaaagttc ttctgaccga
     4021 agaaatgaaa aagaaacgag aggatgaaat aatagcccaa gagcgtcaag ttaaggaact
     4081 acaaatgaag tactttggcc gcgacgggtt acttttcaaa aaacgggagg aactggttaa
     4141 acccatacag gatcaggttt ttaacgcaat caaagagatt gcagttgaag gggggtatgc
     4201 cgtaatattt gacgctgccg cttcacctaa catgatttac accaacccac gattcgacaa
     4261 gagcgaagaa gttctccagc gcttaggttt taaataattt ttatatattt gcttaaacta
     4321 aaaacgataa acaatgagaa agattcttgg attagcaata gttgctctac taacgtttag
     4381 tttttcagga gtaaacgccc aaaactataa atttggtcat attaacagcc aggaactact
     4441 taaagctatg cccgacaggg atagcgccga agctaaaatt agaaaatacg ccgaatcgtt
     4501 agaggatcag attgagcaac ttcaggtaga gttcaacaag aaatatcagg attacctgca
     4561 aaaacgtgcc accttcaccg atgcaatacg tgagatgaaa gaaaaggaac tcaccgatat
     4621 gcagcagagg gctcaggaat accagcaggt tgctgagcag gattaccagc gctaccaagt
     4681 tgaaaccatg aaacctgtaa ttgataaagc cgacgaagcc attaagaaag ttgccaaggc
     4741 aaatggattt acttacattt tcgataccag cacaggggtt ctcctttact tctcggaaca
     4801 gagtatcgat attactccgt tggttaagaa agagttaggc attaccaagt aaacctacaa
     4861 tacctttaaa tttaggggat aaactgaact ttgttcgttt aacccctatt ttatttagga
     4921 agcaaatgag cggcaattgt aatccaatag gaattttcga ctccggtgcg ggtggtctaa
     4981 cagttctttc agaactggtt aaggttatgc ccaacgagaa gtacatatac tttgccgatt
     5041 cagcgaattg cccatacggc tccaaacctg cagaggaaat tatcaaccta acggacaaga
     5101 taacacgctt cctaacaaca aaaggttgca aggttatagt tgtggcctgc aacacagcaa
     5161 cggcagctgc aatagattac ctgcgaaact catacagcat accatttatt ggaatggaac
     5221 cagcagttaa acccgcatcg caaaatacta aaaccaaaag cataggggtt ttggctacgg
     5281 ctggaacttt taaaggacgg ttgtacattg aaaccagccg aaagtacgcc gccgatgtta
     5341 acgtatgtta ccaggtgggc gaagggttag tagaactagt tgaatcggga aaagctgatt
     5401 cccctgaggc agaacagctc ctgcacaaat atatcgaccc aatggttgat tgtaatattg
     5461 accatctggt tttgggctgt acccattatc cattcctaaa acctgtgctg gagagaatgc
     5521 tacctaatca tgtcaccatt gtggaccctg caccggcagt tgccttgcaa actaagaggg
     5581 tagccgaaaa tagcgggttg ctttgtaatc aaaatctagt agatagcacc agtatcgact
     5641 tttactcaag cagtaatacc gaggtactta agatactcgt tgaaatgaac attagaccac
     5701 aatataggat tgaagattta tactttaaca atgtgaaact gtaaaaaacc tattgtatct
     5761 ttaaataaat attactcata tgcgcaaaga ggttgaaata gttattgaga ataccgggca
     5821 aaaggttcgt gtaaccccgg gacactcact tctggaaatt tcaaaaatat tcgaaataaa
     5881 atccgattac cagatcctgg gggcaatggt taacaaccag gtcaaggagt tagactatga
     5941 ggtttttaca cccaaaacag ttcgatacat caatattact caccctgcag gaatgcggat
     6001 gtaccaacga tcgctatttt tcctgctaca aaaagctgtg cgcgatatta tccccggtgc
     6061 caaggtacga attgagcatt ccgtatccaa aggcttctac tgcgagattg acggctgcaa
     6121 cctgcccctg gaattaccgc t
//
LOCUS       DAOOWJ010000132         6112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_12989_length_6112_cov_8.529800, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000132 DAOOWJ010000000
VERSION     DAOOWJ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12989_length_6112_cov_8.529800"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..691)
                     /locus_tag="PLW22_10525"
     CDS             complement(215..691)
                     /locus_tag="PLW22_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75178.1"
                     /translation="MIIKKIAFYIVIATIFFFLTNGSCDKTECGEFWFINRTTHRVKI
                     NSYTYFGLITDMSYEIQQIDSISKVTCDMGMPSNPFTSNYFQVDSIVIVFDDTLSINI
                     FKYLEYNPMNFKVRKKNKFVYEITDSLYCKTLEINGYSSKIVSLPVNGRGTLTQFE"
     gene            896..4111
                     /locus_tag="PLW22_10530"
     CDS             896..4111
                     /locus_tag="PLW22_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU75179.1"
                     /translation="MRDFATVSMSSDSQNNNSYSNWDNFDSNFLIPLIFQNESSIYLI
                     QNFRVVFANPSFTKLTGYAQDELNEILFLDVVHPKDRKLVKILFNNDFAEIRKHTSNS
                     FTLRILTRYNELKWIKTIFSIIDWQESPALLCTSYDITQQKEAEESMANQEQNLSMLV
                     NAFGDLVFIVNHNYNIVQINNAVLSALGHHEHEILLESFVKLHAESERGIAITSLMEV
                     FDGDRKLYITEFVRKNGKPLPLEVRMIKGYWRKRDVVFVIARDITERIEAEAAIKQSE
                     EKFFKAFNSGAVMMTISTLNEGTYIDANRAFLEKIGYDYSEVVGQKSSELRIFKQIER
                     RNELIDAVKRENRVDKIEVEIVNKKGETFTTLLSAEIINIQGTDCLLVAMSDITYRKQ
                     VEEELARSRAQLRGTIDNLPFIAWLKDSKCGYLLVNKKFTNHFGITDDQILGKVDTEP
                     WPSPLLDTLKQKENEVLKSKQTVKWEIREGERRIEEWWEFQLTPVFNVNKKVIAITGI
                     ARKITDQKINQIKIQKHLDRQILLTKVSYVFNKNLPFSKQANESLNLIVSEIGLRKAF
                     ILIGSESDFDIFTCCSSTANDLAEPLRVYYSDNYTSITSHFQQENKVIFDISGSTMPS
                     YDNLKKLTSSQHLLLFPIRVKEKCQGIFAVDYPYGDKVKAADDRDFLLTMSNILSASY
                     ESHLNEEQLRKAKELAEKASQAKEKFLSTMSHEIRTPMNAIIGMANLLIDEDPKPEQV
                     DNLNSLKYAAENLLSLLNDILDYSKIEAHKLDLLQSNFDINDLFKGLYSTFAKMASSK
                     GLTLTYNIDTNIPTPLIGDRVRLNQVLTNLIGNAIKFTEKGEISFTAKLVEKNKEIST
                     IRFTVSDTGIGIPKKMQVDIFNEFTQVHEGKFRATGTGLGLAISQRIVKLMGGEISLE
                     SDEGKGSSFFFTIPFKVGTVQPTDDTVEINIPPGKHRVLIVEDNELNTFIVKRFLTNW
                     GIDFEHASNGREALSCLAQNDFDLILMDLEMPEMNGYEAAKAIRKLHNKAKASIPIIA
                     LSASALLNVQQRIFGIGMNDFVLKPFKPQELKSKLAKYLLK"
     gene            4120..5496
                     /locus_tag="PLW22_10535"
     CDS             4120..5496
                     /locus_tag="PLW22_10535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU75180.1"
                     /translation="MGKNHLSVLASTVIAAISITCHAQVRVELKVHQSIGSTAIVSRY
                     EGKNQIEVDSCKPTPDGVYTFSVPADTPKGIYKVSVGKGASFDFIVSTDTIVRFETYS
                     FAVEDSLKVKQSAENEVFINFRKLRQKAEQKMWLIESLRKYYTEPTMFSQMLENEHQR
                     TAFDLYMQGNALASKANSSLVSSAIRLELTPQPITQGDECSVKKELSEIWWQGIDLTN
                     PDIHFLPRLIPRLWDYLENLLCEGNYTKEEQDSVLSEYIQKLFNLPMHSEIKSLLLSS
                     LCNGFAESDYYGVISTLQQLNENKICPIFNDPELKAKLVLEAGLMPGKKAFDFTFKPV
                     GQKKSIKLSNSTSKYTLVLFWSVWCPHCIESVPQIYKTYKQYQGKGFDVIAICIDDED
                     DAFQNFIKQNELHWKNIRIPYDSNSKVILKYNVDETPKMFIVDKKLNILSRPSTPEHV
                     KVFLEKNL"
BASE COUNT         2023 a         1227 c         1198 g         1664 t
ORIGIN      
        1 cgcagcagga ccggatatat accctatgat ccgtggcatt ttcaaacctg taatcttcac
       61 atacatcctt atctccacaa gaatttagga aggatacaat tacagcaaat aggtataatt
      121 tatatgcttt catcgctttt aaaatttaaa aaagtatgca ttttttttta ttagtaagcc
      181 ccttagagct tttagtaata attttggcac tgaactattc aaattgagtt aaagtccccc
      241 tgccgtttac tggtagggaa acaattttac ttgagtaccc gtttatttca agagttttgc
      301 aataaagcga atcggtaatt tcataaacaa atttattctt tttccgaact ttaaagttca
      361 tgggattgta ttctaaatat ttaaatatgt ttattgaaag agtatcatca aacacaataa
      421 ctatagaatc tacctgaaaa tagtttgatg taaaaggatt cgaaggcatg cccatgtcgc
      481 aggtaacttt tgatatggaa tcaatttgct gtatttcata actcatatca gttattaaac
      541 cgaaatatgt atatgaatta attttcaccc tatgtgtagt acggtttata aaccagaatt
      601 ctccacattc tgttttatcg caactgccat ttgtaaggaa gaaaaatata gttgctatca
      661 caatgtaaaa agcaatcttt ttgattatca caatattaaa tatttgaaca aaaatcaatt
      721 aaattatcaa tatcatcgtt tttttttaca ttttgaggtt tattttgttt tagcaaagat
      781 cttaaaaaga gaaaattcgt aaccccaatt ttttcactaa aataataatt ttaatgatat
      841 gaacaagagg ccaaattatg cgaagcaaca aactgtaaac atttttttgt aacttttgcg
      901 ggattttgcc acggtaagta tgagctctga tagccaaaat aataactcct acagtaattg
      961 ggataatttt gattccaact ttctgatacc cttaatcttt cagaacgaga gcagcatata
     1021 cctaatccag aactttaggg tggtttttgc caatccaagt tttactaagc taaccggcta
     1081 tgcacaggat gaactcaacg aaatactttt ccttgacgtg gttcacccca aggacagaaa
     1141 gctggttaaa atactgttca acaacgattt tgccgaaatc cgcaagcaca catccaacag
     1201 ctttaccctg cgtattctaa cccgctataa cgagctaaaa tggattaaaa ccattttttc
     1261 aataattgat tggcaggaat cgccggcact cctatgtact agctacgaca ttacccaaca
     1321 aaaggaagca gaggagagca tggccaacca ggagcagaac ctgagcatgc tcgtgaacgc
     1381 ctttggcgat ttagttttta tcgttaacca caactataac attgtacaaa taaacaacgc
     1441 cgttctgagc gcccttgggc atcatgaaca cgagatactg cttgagtcgt ttgtcaaact
     1501 acatgccgag agtgaaagag gtatagccat aacctcgctt atggaagttt ttgatggcga
     1561 ccgcaaacta tacatcactg agtttgtgcg taaaaacgga aagcctctcc cgcttgaagt
     1621 tagaatgata aagggctact ggcgcaagcg cgatgttgta tttgttattg cacgtgacat
     1681 tactgaacgc attgaggccg aagcggccat taagcaatcg gaagaaaagt tcttcaaagc
     1741 attcaattct ggtgctgtaa tgatgaccat tagcaccctt aacgagggta catacattga
     1801 tgcaaacaga gcgtttttgg aaaagatagg atacgattac tctgaagttg ttgggcaaaa
     1861 atcatcggaa cttagaatat ttaagcagat tgaacggcgc aatgagctga ttgatgctgt
     1921 aaagcgggag aacagggtcg ataaaattga ggtggagata gttaataaaa agggtgaaac
     1981 ctttacaacg cttctctccg ctgagattat caacatacag ggcaccgatt gcttgcttgt
     2041 tgccatgagc gatataacct accgtaaaca ggttgaagaa gagcttgccc gtagtagagc
     2101 acagctaaga ggaacaattg ataatttacc tttcattgcc tggctcaagg atagcaaatg
     2161 tggctatttg ctagtgaaca aaaagttcac caaccacttt ggaatcaccg atgatcaaat
     2221 tttgggcaag gtagatactg aaccttggcc ctcgccactt ctcgatactc tcaaacagaa
     2281 ggaaaacgag gtgctaaaaa gcaagcaaac tgtaaaatgg gagattcgtg aaggcgaacg
     2341 cagaattgag gaatggtggg aatttcagct aacacctgtg tttaatgtga acaaaaaggt
     2401 aattgccatt actgggatag cacgaaaaat aaccgaccaa aaaattaacc aaataaaaat
     2461 ccagaaacac ctcgaccggc aaatactgct cacaaaggtt tcgtacgttt ttaacaaaaa
     2521 cctccctttt tcaaagcagg caaacgaatc gttaaacctt attgtaagcg aaattggttt
     2581 acgaaaggcc tttatcctta ttggtagtga aagtgatttt gatattttta cttgttgttc
     2641 aagtacggct aatgatttgg ctgaaccact ccgggtatac tactccgata actacaccag
     2701 cattacttca catttccagc aggagaacaa agtaattttc gacatttccg gctcaaccat
     2761 gccctcgtac gacaacctta aaaaattaac cagcagccaa cacttactgc tcttccccat
     2821 tcgtgtaaaa gagaaatgcc aaggcatttt tgcagttgac tacccttacg gcgataaggt
     2881 taaagccgcc gacgacaggg atttcctttt aaccatgtca aatatcctgt cggcctcata
     2941 cgagtcgcac ctgaacgagg aacaactccg taaagccaaa gaacttgccg aaaaggcaag
     3001 ccaagctaag gaaaagttcc tttcaactat gagtcatgaa attcgtacgc ctatgaacgc
     3061 cataataggt atggcaaacc ttttaattga tgaggatcca aaacccgagc aggttgacaa
     3121 cctaaactcc cttaaatacg cagctgagaa tctactttcg ctcctgaacg acatactaga
     3181 ttacagtaaa attgaagccc ataagctcga tttacttcaa tccaactttg acatcaacga
     3241 tttgtttaaa gggctataca gcacttttgc taaaatggca tcgagcaagg gtttaaccct
     3301 gacctataac attgacacca atattccaac cccactcatt ggcgaccggg ttcgtttaaa
     3361 tcaggttctt accaacctta ttgggaatgc cataaaattc actgagaagg gcgaaattag
     3421 cttcaccgcc aaactcgttg aaaaaaacaa ggaaatcagt acaattaggt ttactgtatc
     3481 cgataccggc attggaattc ctaagaaaat gcaggttgat atttttaatg agtttaccca
     3541 ggtacatgag ggtaagttta gagctaccgg cactggttta ggtctagcca tctcccaaag
     3601 aattgtaaaa ctgatgggtg gtgaaattag ccttgaaagc gacgaaggca aagggtcaag
     3661 tttctttttt accatcccgt tcaaagttgg aacagttcaa cccaccgatg atacagtaga
     3721 gatcaacatt ccacccggga aacatcgggt tctgattgtg gaggataatg aactcaacac
     3781 gttcattgtt aaacgtttcc ttaccaactg gggtatcgat tttgagcatg caagcaatgg
     3841 gcgtgaggcc ttgagctgtt tagcacagaa tgatttcgat ttaattctaa tggaccttga
     3901 aatgcccgaa atgaacggct acgaggcagc taaggccatc cgcaagttac acaataaggc
     3961 caaagcctca attccaataa ttgcccttag cgcctctgct ttactcaatg tgcagcaacg
     4021 tatttttggc atcggtatga acgattttgt gcttaaacct ttcaaacctc aggaactaaa
     4081 aagtaaactg gcaaagtatc tgcttaaata gcgtaaaata tgggtaaaaa tcatttatcg
     4141 gtattggcat caacggtaat agcggcaatt tcaattacat gtcatgcaca agtaagggtt
     4201 gagttaaagg ttcaccaatc cattggtagc acggcaatag tatcgcgtta cgagggtaaa
     4261 aatcagattg aagtggactc gtgcaaacca acccccgatg gtgtttacac ttttagtgtc
     4321 cctgcagata cacctaaagg aatttacaag gtatcggtag ggaaaggggc ctcgttcgat
     4381 tttattgttt caaccgatac cattgtgcgc tttgaaacct attcatttgc cgttgaggat
     4441 agcttaaagg taaaacaatc ggccgagaac gaagttttta taaacttcag aaagctaagg
     4501 caaaaggctg agcaaaaaat gtggctaata gaatcgctcc gtaagtatta cactgaacca
     4561 actatgttta gccaaatgct cgaaaacgag catcaacgaa cagctttcga cctgtatatg
     4621 cagggtaatg cgcttgcttc aaaagccaat agttcgctgg tatcgtcagc cataaggctt
     4681 gagctgacac cccaaccgat tacccagggc gatgaatgct cagtgaaaaa agagctatcc
     4741 gaaatatggt ggcaaggaat tgaccttacc aatcccgaca tacatttttt gcctagactt
     4801 ataccccgat tatgggatta cctggaaaac ttgctatgcg agggcaacta taccaaggaa
     4861 gaacaggact ctgttttatc ggaatacatc caaaaactat ttaacctgcc catgcattcc
     4921 gaaattaaat cgttactatt aagctcactt tgtaacggtt ttgctgagtc ggactactat
     4981 ggagtgatat caaccctgca gcagctgaac gaaaataaga tctgccccat ttttaacgac
     5041 cccgaactca aagcaaaact tgtccttgaa gcggggctaa tgcccgggaa aaaagcattc
     5101 gactttacat ttaagccagt gggtcagaaa aaatcgataa aactatcaaa tagcacatca
     5161 aaatacactt tggttctatt ctggtcagtt tggtgccccc actgcatcga aagcgtacca
     5221 caaatctata aaacctataa gcaataccag ggtaaaggct ttgatgttat agcaatatgc
     5281 attgacgatg aggacgatgc ctttcaaaac ttcatcaaac aaaatgagct gcactggaag
     5341 aatatcagaa ttccttacga tagcaatagt aaggtaattt taaaatacaa tgttgacgaa
     5401 acccctaaaa tgtttatagt tgataaaaag ttgaatattc tatcgcgccc atctacgcct
     5461 gaacacgtta aagtttttct tgaaaagaac ctgtaattag ctaaacagaa gttccatgaa
     5521 ttaacaaatc caacttgcct gattttagag gaatgaggtg gaatgaacct gaataagtcg
     5581 gaatgaatcg gaatgaatca gaataagaag aatatgatgg agttagagga agttagaaga
     5641 atttctagtc cgttaaacgt gaaccgtgag ccgttaaccg ttaaacggtg tctagtcctg
     5701 atacacggaa taggcagtat cgagaaatca cattaataag tgaatgaagg tacaaagtga
     5761 tgcttgggct agctcagcat gacatcaagg ctaaattctc ttctgctaac aaccatcaac
     5821 catcaactat caactaaact ggtatcacag agtttcacaa tgtaacacgt tgtttcacag
     5881 agtgaatgat acggtattcg cggataacga ttcacgaaca acaatgagca ccgtttccag
     5941 ccctgtaggg gcgtaatatt tgtaactcca tgattattcc cacatgtata gcgatgcacg
     6001 caatacattt tcgagagagc aaggggaaag tgttgcatcg cagaagaaaa gcattataaa
     6061 gagcaattta cacgttcttt cttgtcttga tacaagaaag aaccaaagaa aa
//
LOCUS       DAOOWJ010000133         6059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_13169_length_6059_cov_8.524317, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000133 DAOOWJ010000000
VERSION     DAOOWJ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13169_length_6059_cov_8.524317"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1962)
                     /locus_tag="PLW22_10540"
     CDS             complement(<1..1962)
                     /locus_tag="PLW22_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75181.1"
                     /translation="MRVTVITFLTLLASLTFGQQVVTFNFESGDLSEWSQKPENRWSI
                     STNSPLSGSASLRHSFDNSIAYTDTIYRALPVWDIEKGDVTWRFLIRHGYDPSASNSW
                     WVYLMANDEIANSNESGYAVGVNLTGSDDLLKLWRIDNGSAQVILTSTLNWQTQIGTG
                     KAGAIEVTRTFDGNFTLRASVSGNFSTLTTHGTVYDFSYKNFNIFGIGYRYSSAQDMK
                     LWADDISFIYQPINPNDRTTAVLNPSQQVVPTNIISTSTLEENCVDVLRFRITDAGSG
                     DGLPTYVKRMTIKNPDPENASWSSLLGGITLKKDGQLIPTQTVSITDQQVTIEVDSSL
                     ATVPDGQYTEFTFGVYLKDSNIPDNLNLLFEIDNENHGFQSSLWGSGFAQTFPAKVQS
                     GIFTIRVNATQAMFRNISPIAIIGKPFGLEVAATDTNSNTDADYGGQITLSLSEGNGS
                     LSSVNGLSRVAIQGIAKWDSLLYDRYGSIRIMAAGENINTDISDEIVIGYDTTSYAAV
                     PGNQVSSFDISSMATSPSQANELMRFRITDAGSDGAPTHLMKIRLNRANTVNAATLNK
                     AIEGVMVKLGDNYISFSSVDIKTSTIDITFPPGALTIPDGQTIELGIWVYLKNSGISD
                     NQTIQLYIDKLNPGFEAYDIGSRFATKFPT"
     gene            2035..3027
                     /locus_tag="PLW22_10545"
     CDS             2035..3027
                     /locus_tag="PLW22_10545"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOU75182.1"
                     /translation="MRVAVVGATGLVGSVMLRVLEERNFPVDELIVAASEKSVGKTVK
                     FKDAEVKVCSVADAVKQKPAVAIFSAGSAASKEWAPKFAEVGTTVIDNSSCWRMYPEI
                     PLVVPEVNAGVLTPNHKIIANPNCSTIQLVVAINPLHLKYRIKRIVVSTYQSVTGTGV
                     KAVNQLFAERQGHEADMAYPHPIDLNCFPHGGTFLDNGYTTEEQKLIDETRKIIGDQS
                     IMVSPTVVRIPVIGGHSESVNLEFESEFDINEVNEILRSAPGVVVQDNPATNHYPMPR
                     FAEGKDDVFVGRIRRDVSRPRSLNLWVVSDNLRKGAATNAIQIAEYLNAKGWLR"
     gene            complement(3008..4225)
                     /gene="hflX"
                     /locus_tag="PLW22_10550"
     CDS             complement(3008..4225)
                     /gene="hflX"
                     /locus_tag="PLW22_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOU75183.1"
                     /translation="MINPISTAKKQERAVLVGVLLPFQKEQEITDYLDELEFLTETAG
                     AITLKRFIQKIDEPNPRTFIGSGKVQEIAAYIDDNDVDMVIFDDELSPTQLRNLEHEF
                     KRKVLDRTNLILDVFAQRAKTAYAKTQVELAQYQFLLPRLKRMWTHLERQRGGIGLRG
                     PGEREIETDRRIIRDRIAKLKEELSKIDRQMATQRKNRGSVVRVALVGYTNVGKSTIM
                     NLISKSDVFAENKLFATLDTTVRKVVIGNLPFLLSDTVGFIRKLPHQLVESFKSTLDE
                     VREADLLLHVVDIAHPNFEEQINIVNQTLHEIGGGDKPTILVFNKIDAYTWTRKPDDD
                     LTPSTRENISLEELKRTWISKKETHTIFISAKQKINIDEFRALLYKMVREIHVTRYPY
                     DNFLYPDITEANL"
     gene            complement(4293..4973)
                     /locus_tag="PLW22_10555"
     CDS             complement(4293..4973)
                     /locus_tag="PLW22_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOU75184.1"
                     /translation="MEAKKSKKADLESKRILFFEVGLTVSILLAILAFEWKSPAGINT
                     SLIAELGNNNGTIELPPITTDPKEMVKPEPPKVVISEKIIVVDNNVEPNYNTDIFEEP
                     KDAYVPPVIRIEEKEVDETFERVEVMPTFDKGDANHFRNTYVIPNIRYPQEAIDNGVA
                     GVAFVEFVVERDGRVTNVKVLNKIDKSLEREAIRVVSSSPRWEPGFNNGVKVRVKFVI
                     PIKFVLNN"
     gene            complement(5093..5779)
                     /locus_tag="PLW22_10560"
     CDS             complement(5093..5779)
                     /locus_tag="PLW22_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOU75185.1"
                     /translation="MELKKNPKADLQNKKLLFFEIGLALSLGIVLLAFEWTTTDTVNV
                     DLAQTTEVKVEQEIIPITNRDEVKPPEPPKQITVSDVIKVVDDNVNINDNTDIFDSEF
                     SEDKAVTVMEFKDEEQEEEEYTPFVIVEDMPSFQGGDINNFRVWVQQNLKYPDVAAEN
                     GIQGRVIINFVVEPSGKVTNVKVVRGVDPSIDKEAIRVVSSSPIWKPGSQRGKPVRVQ
                     FTIPIIFVLQ"
     gene            5961..6034
                     /locus_tag="PLW22_10565"
     tRNA            5961..6034
                     /locus_tag="PLW22_10565"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5995..5997,aa:Ala,seq:ggc)
BASE COUNT         1586 a         1332 c         1346 g         1795 t
ORIGIN      
        1 ggttgggaat ttagtagcga atcggcttcc tatatcgtag gcctcaaaac ccggatttaa
       61 cttatcgata taaagttgaa tggtttgatt atcggatatt ccgctattct tcaagtacac
      121 ccatataccc aattcgatgg tttgcccatc ggggatggtt aatgcgccag gagggaatgt
      181 aatatcgatg gttgaagttt ttatatcgac actggaaaat gagatatagt tgtccccaag
      241 ttttaccata acgccctcaa ttgctttgtt tagcgttgcc gcatttaccg tatttgccct
      301 gttcaaacgg attttcatga ggtgggttgg tgcgccatcg ctgccggcat cggtaatgcg
      361 gaatcgcatt agctcattag cctgtgaggg acttgtagcc attgaactga tatcgaacga
      421 tgacacctga tttcccggaa ctgccgcata cgaggtggta tcgtaaccaa taactatttc
      481 atcgctaata tccgtgttta tgttttcacc agcggccata attcggatac tgccgtacct
      541 gtcgtataaa aggctgtccc atttggcaat gccctgtata gctactcggc taagcccatt
      601 tacagagcta agacttccgt tgccctctga aaggctcagg gttatctgac ctccataatc
      661 ggcatcggta ttactattag tatcggtagc agctacttcc agaccaaagg gtttgccaat
      721 aatggcaata ggcgatatgt tgcggaacat agcctgtgtg gcgtttaccc taatggtgaa
      781 aatacccgat tgcaccttgg ccgggaaggt ttgagcaaat cccgaccccc acaggctcga
      841 ttgaaaacca tggttttcgt tatctatctc aaacaaaagg ttgaggttat cgggaatgtt
      901 gctatcctta agataaacac caaatgtgaa ctccgtatac tggccgtcgg ggactgtggc
      961 aagcgagcta tccacctcaa tggttacctg ttggtcggtt atgctcacgg tttgagttgg
     1021 gataagttgc ccatccttct tcagggttat tccgccaagc aggctactcc acgatgcgtt
     1081 ttcagggtcg ggatttttga ttgtcattcg cttaacatat gtaggcaaac catcgccaga
     1141 tcccgcatcg gtaatacgaa accgtaaaac atccacgcaa ttttcctcta atgttgatgt
     1201 agatattata ttggttggaa caacctgttg cgatgggttt aacactgctg ttgtacggtc
     1261 gtttgggtta attggctgat agatgaacga aatatcatcg gcccagagct tcatgtcctg
     1321 tgccgatgag taacggtaac caatcccgaa aatgttaaag ttcttgtagc taaaatcata
     1381 aactgttccg tgggtggtta aggtgctgaa gttaccgcta actgatgccc ggagggtaaa
     1441 atttccgtca aaagttctgg taacctcaat cgctcctgct ttcccggtgc caatttgtgt
     1501 ttgccagtta agagtggagg ttagtataac ctgagccgaa ccattatcga ttcgccaaag
     1561 ctttaatagg tcatcgctgc cggttaggtt aacacctaca gcgtaccccg attcatttga
     1621 atttgcaatt tcatcgtttg ccatcaggta aacccaccag ctattcgatg ccgatgggtc
     1681 gtaaccgtga cgtatcaaaa atcgccatgt aacatcacct ttttcaatat cccacaccgg
     1741 taaagcccta taaatggtat cggtgtatgc aatgctattg tcaaagctgt gccttagcga
     1801 ggcgctccct gagagtggac tgtttgtgct aattgaccaa cggttttcgg gtttctgaga
     1861 ccattccgat aaatcacccg attcaaaatt gaaggttacc acttgctgcc caaaagtcaa
     1921 gctggcaagt agtgttaaaa aagttatgac cgttactcgc atttaaacta ttgtataatt
     1981 ttacaatcaa aatttacgaa aaaattcaat taatcaccta taaaatattt atttatgcga
     2041 gtggcagtag taggggcaac aggcctcgta ggtagtgtta tgcttcgtgt tctggaggaa
     2101 cgcaatttcc ctgttgatga gttaattgtt gcagcatcgg agaaatcggt tggtaaaacc
     2161 gtaaagttta aggatgccga ggtaaaagtt tgctccgttg ccgatgccgt taagcaaaaa
     2221 cctgccgttg caattttctc tgcaggttca gccgcatcaa aggaatgggc tccaaagttt
     2281 gccgaggtgg gcactaccgt tatcgataac tcatcgtgct ggcgtatgta ccccgaaatt
     2341 ccacttgttg ttcccgaggt taacgcagga gttcttaccc caaaccataa aattattgct
     2401 aacccgaact gttcaaccat tcagctggtt gtagccataa acccccttca ccttaagtat
     2461 cgtattaaac ggattgttgt ttcaacctac cagtcggtta caggaaccgg cgtaaaagcg
     2521 gtaaaccagc tttttgctga gcgccaaggg cacgaggccg atatggcgta ccctcatccc
     2581 atcgacttaa actgttttcc gcatggcgga acatttctcg ataacggcta cactaccgag
     2641 gagcaaaagc tgattgatga aacccgcaag ataattggcg atcagtctat catggtatcg
     2701 ccaaccgttg ttcgcattcc ggttattggc ggccactccg agtcggttaa ccttgagttt
     2761 gagagcgagt ttgatatcaa tgaggttaat gaaattttac gtagtgcacc aggtgttgtg
     2821 gttcaggata atccagccac aaaccattac cccatgccac gttttgccga gggcaaggac
     2881 gatgtatttg tggggcgaat ccgacgcgat gtttcccggc cgcgaagcct caatctttgg
     2941 gtagtatcgg acaatcttcg caagggcgct gccaccaatg ccattcaaat tgccgagtac
     3001 ttaaatgcta aaggttggct tcggtaatat cggggtatag gaaattgtcg taggggtacc
     3061 gcgttacatg aatctccctg accatcttat acagtagcgc acggaactca tcaatattga
     3121 ttttctgctt tgcagaaatg aaaatggtat gggtttcctt tttgctaatc caggttcgtt
     3181 tcagctcctc aaggctaatg ttttcgcggg ttgaaggtgt aaggtcatcg tcgggtttac
     3241 gggtccaggt gtaggcatca atcttgttaa acacaagtat agttggctta tcgccgccgc
     3301 caatctcgtg taatgtttgg ttaacaatat tgatttgctc ctcaaagttg gggtgggcta
     3361 tatcaactac atgcagcagc aggtcggcct ctcgtacctc atcgagggtt gacttgaacg
     3421 actccaccag ctgatgtggt agctttcgga taaaaccaac ggtatcggat agcaggaaag
     3481 gcagattgcc aattaccacc ttacgcactg tggtatcgag ggtggcaaaa agtttattct
     3541 cagcaaatac atccgatttg ctgatgaggt tcataatggt ggacttaccc acgttggtgt
     3601 agccaaccag cgcaacacga accacgctcc ctcgattctt acgttgtgta gccatctggc
     3661 ggtcaatttt gctcagttcc tcctttagct tggctattcg gtcgcgaata atacggcggt
     3721 cggtttcaat ttcacgttcg ccagggcctc gtagcccaat acctccgcgc tgacgctcaa
     3781 ggtgagtcca cattcgtttt aggcggggaa gcaagaattg gtactgtgcc agctcaacct
     3841 gggttttggc gtaagccgtt tttgcccgtt gggcaaacac atcgagtatt aggttggttc
     3901 gatccagcac tttgcgttta aattcgtgct caaggttacg gagttgggta ggagagagct
     3961 catcgtcgaa aattaccata tccacatcgt tatcatcaat ataagcagca atctcctgaa
     4021 ccttaccgct accaatgaat gtgcgtgggt tgggttcatc aatcttctga ataaaccttt
     4081 ttagggttat ggcccctgct gtttccgtta ggaattccag ctcatcgagg taatcggtta
     4141 tctcctgttc tttctgaaaa gggagcaaaa caccaaccag tactgctctt tcctgttttt
     4201 ttgctgtcga aattgggttt atcatttgcc tgtatcaata aaaaaacctg aataatcagg
     4261 gttttcttta acgattttat caaagtaaat gtttaattat tgagcacaaa tttaatagga
     4321 attacgaatt taaccctaac ctttacacca ttattgaatc ctggctccca gcgaggcgaa
     4381 ctcgaaacaa cccttatggc ctcccgctca agacttttat ctattttgtt taatacctta
     4441 acgtttgtta ctctgccatc gcgctcaaca acaaattcaa caaaagcaac tcctgctacc
     4501 ccattgtcaa ttgcttcttg tgggtaacgg atatttggaa taacatatgt gttacggaaa
     4561 tggttggcat cgcccttgtc aaaggttggc atcacctcaa cacgttcaaa ggtttcatca
     4621 acttcttttt cctcaatccg aataactgga ggaacataag catccttggg ttcctcaaaa
     4681 atatcggtat tatagtttgg ctctacattg ttatcaacca caataatctt ttcggagatt
     4741 accactttgg gcggttctgg ttttaccatc tctttagggt cagtggtgat aggcggtaac
     4801 tcaatagtgc cattattgtt accaagctca gcaatcaggc tagtattgat accagcaggg
     4861 cttttccatt caaaggccag gatagcaagt aggatcgata ctgttaatcc tacctcaaag
     4921 aagaggatac gcttgctttc caggtcggct tttttcgatt tttttgcttc cataactttt
     4981 tatgttaaag ggtttctagt aataaaacga taatcatacc cctttcattg tgtataataa
     5041 aaaaataacc ccgcaaaaag cggggttaag tattctaatc ttatttaata aactactgaa
     5101 gcacaaagat aattggaatg gtaaactgaa cgcgaacggg tttaccgcgt tgtgaaccag
     5161 gtttccaaat aggtgacgaa gaaaccacgc gtatagcctc tttatcgata gaggggtcaa
     5221 cgccacgtac aaccttaacg ttggtaacct ttccgctagg ttcaactaca aagttgataa
     5281 ttacgcgacc ctgaataccg ttttcagcag ctacatcggg atacttgagg ttttgctgta
     5341 cccaaacgcg gaagttattg atatcgccac cctggaacga gggcatatcc tctacaataa
     5401 cgaatggagt gtactcttcc tcttcctgtt cttcatcctt gaattccata actgtaacag
     5461 ccttgtcctc ggagaattcg ctatcaaaga tgtcggtatt gtcgttgata ttaacgttat
     5521 catcaactac cttgataacg tcagaaacgg taatctgctt gggtggttca gggggtttaa
     5581 cctcatcgcg gttggtaata ggaataattt cctgctcaac ctttacttct gtggtttggg
     5641 caaggtcaac gtttacggtg tcggtggttg tccattcaaa ggccagcaaa acaataccca
     5701 gcgataaagc aagtcctatc tcaaagaata ggagtttctt gttttgcagg tcagccttgg
     5761 gatttttctt tagttccata tttttgcgta tttggctctt ggtataacgg ttaatttcct
     5821 aatttatttt gagctacaaa gcaataaaaa ttttatttat cttccaatga ttatacgtag
     5881 ttttttaaat catgttaaaa aaaattgcgt gaaattggca gtataattaa aaagttttta
     5941 tttttgcagc gttatttcat gggggtatag ctcagttggc tagagcgctt gcatggcatg
     6001 caagaggtca ggggttcgac tccccttacc tccacggttg aaagggatac gcaagtaag
//
LOCUS       DAOOWJ010000134         6059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_13170_length_6059_cov_7.061959, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000134 DAOOWJ010000000
VERSION     DAOOWJ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13170_length_6059_cov_7.061959"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1647
                     /locus_tag="PLW22_10570"
     CDS             <1..>1647
                     /locus_tag="PLW22_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU75186.1"
                     /translation="STRGIALSNLAIGRCYTGLAQFGLAIKYLLEAKRLSKLNGDEDI
                     SAQANRQLSTCYEATGDFKNSQKCLKEFLEYTDKTFTKKLITQVAQLHSMLQIEKQER
                     ENQQLQMQLELKSLNEHKDKKIITLYTIFSILLLLTLSVIVYFAIQLSQKKKLLEHTN
                     SQLFKFNNELDSLVKHRTRELNNAIDKIRELERIKSAFMANISHEIRTPLNGILGLSY
                     YLSAPECTAEERSQIGEQVKRLGNKLVRIVDDILELSKIETNQVNLLLSEVNLNQLLD
                     DVYKEFDAIAEFDDKNLFFRVNKSLPDDRAIVICDYYRLRSIIIHLLENAFKFTHQGG
                     VEMGYSINENSLLYLYVKDTGVGIPNDIQKKVFERFYKHLDDSSPIFYDGLGIGLTIA
                     MGYAIALGGSIKLQSSPGQGSTFTLVLPITVPNSNANKEAFNFTGKRILVVEDDLINY
                     QYLHALLSKTGATVIHVKNGEDAIEAATIERAADLILLDIQLPFKSGIEATIEIRKIN
                     KQIPIIAQTATDSEYVSACRDAGCNAFVTKPIDPDELLNL"
     assembly_gap    1648..1747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1748..2598)
                     /locus_tag="PLW22_10575"
     CDS             complement(<1748..2598)
                     /locus_tag="PLW22_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOU75187.1"
                     /translation="MGLKCGIIGLANVGKTTIFNCMSSTKALTSNVATGTGKSNISTI
                     NVPDNRLYELEKFQPTERIVHTTVEIVDIPGLSKTTGKGEGNKFLADVRNCDALIHVL
                     RCFDDPSLPHIDGSIDPVRDIETVNFELQVRDLESVEKKLQKVEKAAKVGDKEAKKAF
                     EVLSVYKNHLESFKNASTVPVDEHDKKYVDDLFLLTTKPVIYVCNVDEKSAVNGNQYS
                     NRVKEFLKDEKTEILIIAGKVEAEISELESEDDRKAFLADVGLTEPGVNKLIRAAYSL
                     LNLETFFT"
     gene            complement(2678..3709)
                     /locus_tag="PLW22_10580"
     CDS             complement(2678..3709)
                     /locus_tag="PLW22_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU75188.1"
                     /translation="MKQCPKYFKALILGLAILTACVPAKEFQALQDKNAKCEEEREKL
                     TVENEALTTKNSELDSKTRILQAKVDALTSDSINRAVTFNRINEELTTLKERYAELQS
                     LQDGVISGSKDETKKLLLQIQKTQEELQEKEDALKELERRLYQRKLGLDKVQANLDSM
                     KRELDARNARLVELERMLNAKDSVMRALRKKVADALFGFEGKGLTVSIQNGKVYVSLE
                     EKLMFKSGSYEIDSKGAAAIKQLIPVLEQNPDINIMVEGHTDDVPYRGTGGLQDNWDL
                     SVKRATTIVRLLISGSRIDPVRVTAAGRSQYLPIDRAKTPEARQKNRRTEIILTPKMD
                     EILQLLESN"
     gene            3865..5793
                     /gene="dxs"
                     /locus_tag="PLW22_10585"
     CDS             3865..5793
                     /gene="dxs"
                     /locus_tag="PLW22_10585"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOU75189.1"
                     /translation="MVFMENKYALLSNIDSPEDIKRLKPEQLVDLSAELRQYIIDIVS
                     ENPGHFASSLGVVELTIALHYVYNTPFDRIIWDVGHQAYGHKILTGRRDVFHTNRKYK
                     GISGFPKRSESPYDAFGVGHSSTSISAALGMSVASRLKLENRKVVAVIGDGALTGGMA
                     FEALNHGGGINPNMLVILNDNNISIDPSVGALKEYLLDITTSKTYNKLKDEVWDILGR
                     FDRVGPKTRSLIQKFENAIKAALLKQSNLFESMGFRYFGPIDGHDVIYLTRVLQDLKD
                     IPGPKLLHIVTVKGKGYAPAENNQTAFHAPGLFDKETGERIKVDANKPTPPRYQDVFG
                     ETLVELAEQNPKIVGVTPAMASGCSLNIMMQKMPDRAFDVGIAEQHAVTFSAGLAAEG
                     LIPFCNIYSTFMQRAYDQVIHDVALQNLPVVFCLDRGGLVGDDGATHHGAYDLAYMRS
                     VPNMTVAAPMNEEELRNLMYTAQLEPKGPISIRYPRGNGVMVDWRKPFKRVELGKGRV
                     LRTGTDIAILSIGHIGNLAAKAADMLEADGHAVFHADMRFVKPLDVEILEAVGKEFKT
                     VITVEDGCLPGGFGSAVLEWFNDNGYSIKVVRLGIPDTFIEQGTQQELYAECGYDIEN
                     IYKTAKRYITKKVKLRAG"
     gene            5860..5946
                     /locus_tag="PLW22_10590"
     tRNA            5860..5946
                     /locus_tag="PLW22_10590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5894..5896,aa:Leu,seq:caa)
BASE COUNT         1610 a         1340 c         1346 g         1663 t
ORIGIN      
        1 ctccactcgc ggaattgctc taagcaattt ggcaattggc cggtgttata ctggtttagc
       61 gcaatttggg cttgcaataa aatatttact tgaggctaaa cgcctatcca aactcaatgg
      121 cgatgaagat atttcagctc aagctaacag gcagctatca acctgctacg aggcaaccgg
      181 cgattttaag aattcccaaa aatgtttaaa ggaatttttg gagtataccg ataaaacatt
      241 caccaagaag cttataactc aggttgcaca gctacactca atgttgcaga ttgaaaagca
      301 ggaaagggaa aaccagcagt tacaaatgca gctggagctg aaatcgttaa atgaacacaa
      361 ggataaaaag ataattactt tgtacacaat cttttcaatt cttttgctcc taaccctcag
      421 cgtaattgtt tactttgcca tacagctctc ccaaaagaaa aagcttttag agcatacaaa
      481 ctctcagctg ttcaagttca ataatgaact cgattccctg gtaaaacata gaaccaggga
      541 gttgaataat gctattgaca aaataaggga gcttgaaaga attaaatccg ctttcatggc
      601 aaatatcagc catgaaatcc ggacacctct gaatggaata ctaggactct cctactacct
      661 ttcggcgcca gaatgcactg ctgaagagcg ttcgcagata ggcgaacagg ttaagcgttt
      721 gggcaataaa cttgtaagaa tagttgacga tatccttgaa ctttcaaaaa ttgaaactaa
      781 ccaggtaaac ctactgctat ccgaggtcaa cctaaatcag ctgctcgatg atgtttacaa
      841 ggagtttgac gccattgctg aatttgatga taaaaatctc ttttttaggg taaacaaatc
      901 gttgcccgac gatagggcaa tagttatatg cgattactac cgtttgcgta gcattattat
      961 tcatctttta gagaatgcct tcaagtttac ccaccagggt ggggttgaaa tgggttactc
     1021 cataaacgag aactcgttgc tatacctata cgtaaaggat acaggggttg gcatacctaa
     1081 cgatatccag aagaaagttt ttgagcgttt ttataagcac ttagatgatt ccagcccaat
     1141 tttttacgat ggtctaggaa ttggattaac catagccatg gggtatgcca ttgcgttagg
     1201 gggtagcatc aagcttcaat cctcaccagg tcaaggatca acctttactc ttgtgctacc
     1261 tataacagtg cccaactcca acgccaacaa ggaggctttc aactttactg gtaaaagaat
     1321 actggtggta gaggatgatc tgataaacta ccagtaccta cacgctttgc ttagcaaaac
     1381 gggcgctact gttattcatg ttaaaaacgg cgaagatgcc attgaggcag caaccattga
     1441 aagggcagcc gatttaattc tacttgacat tcagttacct tttaagagcg gaatagaggc
     1501 taccattgag atacgaaaga taaataagca aatccctatc attgcccaaa ctgctaccga
     1561 ttccgaatat gtgagtgcct gtagggatgc tggttgtaac gcatttgtta caaaaccaat
     1621 tgaccccgat gaactactaa acctcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnngta aaaaaggttt cgaggtttag cagggagtat gccgctctaa taagcttgtt
     1801 aacgccgggc tcagtaagcc ctacatcggc caaaaaggct ttgcggtcat cctcactctc
     1861 aagttccgat atttctgcct caaccttacc ggcaataatc agtatttccg ttttttcatc
     1921 ctttaggaac tcctttaccc tattggagta ctggttgccg tttacagccg atttttcgtc
     1981 gacattgcat acgtagataa ccggttttgt ggtaagcagg aaaagatcgt caacatactt
     2041 tttatcgtgc tcatcaactg gaacagttga agcgtttttg aacgactcaa ggtggttttt
     2101 gtaaaccgat agaacctcaa aagctttttt ggcttcctta tcgcctacct ttgcagcttt
     2161 ttcaaccttt tgtagctttt tctcaaccga ttccaggtcg cgaacctgca gctcaaagtt
     2221 caccgtttca atatcgcgaa cggggtcaat ggaaccgtcg atatggggta gcgagggatc
     2281 gtcgaaacag cgtagcacgt ggattaatgc atcgcagtta cgaacatcgg ccaggaactt
     2341 attgccctcg cccttaccag ttgttttcga taaaccggga atatcaacaa tctcaacggt
     2401 ggtgtgaaca atacgctcag tgggttggaa cttttcaagt tcgtaaagcc ggttatcggg
     2461 gacgtttatt gtactgatgt tcgattttcc tgtgccggta gccacattgc tggttaaggc
     2521 tttagtgctc gacatgcagt tgaaaatagt agttttgccc acgttggcaa gaccaattat
     2581 gccacatttt agacccattg ctattcaaat tatttgagca caaaattaca gcaaataggt
     2641 cagataacaa aaaaagcggc tactgccgct tttgagttta gttgctttcc agcagctgga
     2701 gtatttcgtc catttttggg gtaagaataa tctcagtgcg cctatttttt tgtcgagctt
     2761 cgggtgtttt agccctatcg atgggaaggt actggctccg ccctgctgca gttacacgca
     2821 cagggtcaat ccttgagcct gaaatcagta ggcgtacaat ggtagtggca cgttttacgc
     2881 ttaaatccca gttatcctgg agccctcctg ttcctcggta tggaacatcg tcggtgtgac
     2941 cctcaaccat tatgttaata tcggggttct gttcaagaac cgggattaac tgctttattg
     3001 cagcggcacc ctttgagtca atctcgtagc tacccgattt gaacatcagc ttttcctcca
     3061 gcgatacgta aaccttgcca ttctgaattg agactgtgag ccccttgccc tcgaacccaa
     3121 aaagcgcatc ggcaactttt ttccgcaggg ctctcataac tgaatccttg gcatttagca
     3181 ttcgctccaa ctcaaccaac cgggcattgc gtgcatcaag ctcacgtttc atgctatcga
     3241 ggtttgcctg caccttatcg agcccaagct tacgctgata tagcctccgc tcaagttctt
     3301 taagggcatc ttccttttcc tgcaactctt cctgggtttt ctgtatttga agtaaaagct
     3361 ttttggtttc atccttgcta cccgaaatta ccccatcctg taggctttga agctctgcgt
     3421 aacgctcctt aagcgttgtt agctcctcat taatgcggtt gaaagtaact gcacggttta
     3481 tgctgtcgct tgtgagggca tcaacctttg cctgaagaat acgtgtttta ctgtcgagtt
     3541 ccgagttttt agttgtaagg gcctcgtttt ccaccgttag cttttcacgc tcctcctcgc
     3601 actttgcgtt tttgtcctgt agcgcttgga attccttggc aggcacgcat gcagttagaa
     3661 ttgctaaccc aagtatcagg gctttaaagt attttggaca ttgcttcatg ttgcaattat
     3721 tttgaacaaa tataaaaatt taattgcacc ctcgcatttt gaaacattct taaaatatca
     3781 acataaaact actatttaga cagaaaagat ttggaaaaat atgtaacaaa gtgctatttt
     3841 agctgttaag gtatagtgag gggaatggtg tttatggaga acaagtacgc attgctaagc
     3901 aatatcgatt cgcccgagga tattaaaagg ttaaaacctg agcagctggt tgatttaagc
     3961 gctgagctca ggcagtatat cattgatatt gtttcggaga atccgggcca ttttgcatcg
     4021 agtttagggg tggttgagct aaccattgcc ctccactacg tttataacac tccttttgac
     4081 cgtataattt gggatgttgg ccatcaggcc tatggccaca aaatacttac tggccgtcgc
     4141 gatgttttcc acacaaaccg taaatacaag ggaattagcg gattccctaa gcgttccgag
     4201 agtccgtacg atgcctttgg cgttggccat tcatccacat ccatttccgc agcccttggc
     4261 atgtcggtag catcgaggtt gaagcttgaa aaccgaaaag tagtggctgt aattggcgat
     4321 ggtgcgctta ctggcggaat ggcctttgaa gcccttaacc acgggggtgg tatcaaccca
     4381 aacatgctag ttatccttaa cgacaacaat atctcaatag atcctagtgt tggggctctg
     4441 aaagagtacc tgctcgatat caccacatca aaaacctaca acaagctaaa ggatgaggtg
     4501 tgggatattc tgggacgatt cgatagggtt ggccccaaga cccgaagcct tattcagaaa
     4561 tttgagaacg ccataaaagc agccttgctc aagcaaagca acctgtttga atcaatgggt
     4621 ttcaggtatt ttggcccaat tgacggccac gacgtgattt accttactcg cgtactccag
     4681 gatttgaagg atatacccgg gccaaagttg ctgcacattg ttaccgttaa gggcaaaggg
     4741 tatgcgcccg ctgagaataa tcaaacagca ttccatgctc cgggattgtt tgataaggag
     4801 accggcgaga ggattaaggt ggatgccaat aagcccaccc caccccgcta ccaggatgtt
     4861 tttggcgaaa ccctggttga gcttgctgag caaaacccga aaatagttgg ggttactccg
     4921 gctatggcaa gcggatgctc cctcaatatc atgatgcaaa agatgcccga tagggccttt
     4981 gatgtgggca ttgctgagca acatgctgta accttctcgg caggtctggc ggctgaaggg
     5041 ctgatcccat tctgcaacat ctactctacc ttcatgcagc gggcatacga ccaggtaatc
     5101 cacgatgttg cactacaaaa cctaccagta gttttctgcc ttgaccgcgg cggtttagta
     5161 ggcgacgatg gcgctacaca ccatggcgct tacgacctgg cttacatgcg cagcgttcct
     5221 aacatgaccg ttgcagcacc aatgaacgag gaggagctgc gtaacttaat gtatactgca
     5281 cagcttgagc ccaagggtcc tatctcaatc cgctatcctc gtggcaatgg cgttatggtg
     5341 gattggcgta aacctttcaa acgtgttgaa ttgggcaagg gtagagttct tagaactgga
     5401 accgatatag caattctgtc cattggacac attggaaacc ttgcggctaa ggcggccgac
     5461 atgctcgagg ccgatggcca cgcagttttt catgccgaca tgcgctttgt taaaccactc
     5521 gatgttgaaa tacttgaagc tgttggcaag gagtttaaaa cagttattac ggttgaggac
     5581 ggttgcctgc ccggcggttt tggtagtgcc gtactggagt ggttcaacga taatggctac
     5641 agcattaagg tagtacgcct gggtattccc gataccttta ttgaacaagg aacccagcaa
     5701 gaactctacg ccgaatgtgg ctacgatatt gagaatatct acaaaactgc caaacgttat
     5761 attaccaaaa aggtgaagct gagggcaggc taagatattg cccaaaactg ttttgacaaa
     5821 ttaaataaac ttattatctt cgcaccgatt ttctacaacg cccgggtggc ggaattggta
     5881 gacgcgctgg tctcaaacac cagtggtagc aataccatgc cggttcgacc ccggccccgg
     5941 gtaccaaagc ctcgaatcat tcggggcttt tttcatttcc agtatcgggt tcaatgccgg
     6001 ttggttaggg aggtttttcg aagggttcag gatgatatgg ggagttgttc gttgttcgt
//
LOCUS       DAOOWJ010000135         5918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_13601_length_5918_cov_7.252772, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000135 DAOOWJ010000000
VERSION     DAOOWJ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13601_length_5918_cov_7.252772"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLW22_10595"
     CDS             <1..804
                     /locus_tag="PLW22_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_091910270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOU75190.1"
                     /translation="KQCHHRQSLRSGTVLENSKLPFMYWYAAMHLLTSTKKSYSAAEL
                     QRQLGHKRYQPIWEMVCKLRDVMGKRYNLYQLTDEVELDNAFIATLIAEEEKDKPLKR
                     GIGSQRQPKAVVVNESSFVENPKPGKKPKRVNHLKMLVIEDLRADTISKTVNEHVNPK
                     AELTTNDATPYSKLFEQVKSHHAQAIKKEDLPKTLPWVNIAIGNVKRLLLDVHHQLTN
                     EYLQYYLNEFCYKFNRRYFGDAFFDGFLIAAVSYNTDFRSRIYNRNSCG"
     gene            825..1637
                     /locus_tag="PLW22_10600"
     CDS             825..1637
                     /locus_tag="PLW22_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU75191.1"
                     /translation="MRKIGLYISVLLSILNYACTDLMEYSPYEGKVQEGSRSLNQTAI
                     GKLQLQSSKTFKPFTFAIIGDSHTYYDDFIKQISVLNNLDSIDMVIHMGDITLSGIYR
                     EFLWYRDIIKPLKYPLITIIGNHDYLSNGEYMYRDMFGPTNFSFTYNNCLLVFFDDIT
                     WERNTKDPDFEWLKSTISATATYTHKFVFTHIPPWDEQFSHGNELYYNYLLAKNNVDY
                     SIHGHIHRFYHDKHYADLYGNVNYLACGDSEDHEVVLVKVKENNIDVEVIHF"
     gene            1640..2281
                     /locus_tag="PLW22_10605"
     CDS             1640..2281
                     /locus_tag="PLW22_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75192.1"
                     /translation="MKTNRFLLLGLLILLYHPRLHAEDPVKGLRQKITEEKKIVLKND
                     TVTIKPYWFMPTQFKVQYAGSIGFVSFGAGYDFRNGYQPTLLYGFLDNNFGGSNITVH
                     TISLKNRFKLTQNPILGCFTPIAGISVNWGITNNTFNRLPPHYPENYYFQNKIHLSPF
                     WGGEFNFSVAQKNLGVYFEFSTLDAYLLEYFRTDFVKLNKIWSLGMGITLYLN"
     gene            complement(2273..3574)
                     /locus_tag="PLW22_10610"
     CDS             complement(2273..3574)
                     /locus_tag="PLW22_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOU75193.1"
                     /translation="MIKRIDNYFELSKANTSIRTEVLAGITTFMTMAYILAVNPSILS
                     NTGMSRDALFTTTALASFLATMIMAIWARLPFALAPGMGLNAFFAFTVVLGMGHSWQF
                     ALTAVFLEGLIFIALTFLNIREKIIDAIPLGIKHAISAGIGLFIAFIGLQNAGVIVKN
                     DNVMVGLGQMGSPSVLLCLAGVIVTGVLLARKVKGALLIGIFLVTLAGIPFGLTELPQ
                     GGLFSMPPSVEPIAFKFEFHTILSIDMLIVLFTFLFVDMFDTLGTLVGVATKANMLDE
                     KGKLPRARQALFADAIGTTLGAMLGTSTVTTYVESASGVAEGGRTGLTSFTTAMLFIA
                     ALFFSPMFLMVPAAATAPALILVGSFMLSPILKIDFNDVTEAIPAFFTIIMMPLTYSI
                     AEGIVFGMLSYVLLKAFTGKVKQVSLVMWVLSLFFLLKFLV"
     gene            complement(3664..4710)
                     /locus_tag="PLW22_10615"
     CDS             complement(3664..4710)
                     /locus_tag="PLW22_10615"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOU75194.1"
                     /translation="MNFFASHWGSIDGKDVMKYSISTEGGLEVSISNLGAAMVAMRLL
                     NDENEPLNLAYGLLRPSDYLKADYYPGVIVGRYANRIANAKFTIGSDEYALCANEGKN
                     ILHGGKIGFSHRVWETVMFEEHNGVCMLEFYLNSLHGDQGFPGNVDVWVIYQITDDNQ
                     VSISYRAVTDRPTHLNLTTHGYFNLSGFETDVLDHMLRIDANHYLPVNSELIPTGEIR
                     EVDGSPFDFRKFKAVGADTDLVGTCYDHCYMLNNPTIDNPSVVLFNPKTHIGLTIYTT
                     QPGIQLYTPMNKPNIANPMIKLPEKGNWAVCMEPQHFPNSPNEGRFPTTLLLPGQEYD
                     HTTILSVTMGGVDK"
     gene            complement(4714..5043)
                     /locus_tag="PLW22_10620"
     CDS             complement(4714..5043)
                     /locus_tag="PLW22_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOU75195.1"
                     /translation="MEVDMPVSEITFWKWFLPKLRNKFFLTVFIFLIWMLIFDSSNWV
                     EMLAAKRRIKSLEEEKVYYLQKIEEDKKKIKELRTNAENLEKFAREQYLMKKPNEDIF
                     IVDETDL"
     gene            complement(5033..5242)
                     /gene="thiS"
                     /locus_tag="PLW22_10625"
     CDS             complement(5033..5242)
                     /gene="thiS"
                     /locus_tag="PLW22_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOU75196.1"
                     /translation="MDITLNNLPEHIDTNKAAITVNELLAFKHFSFKMLVVKVNGKLV
                     KKDEYSMATIKDGDDVQVIHLISGG"
BASE COUNT         1774 a         1307 c         1224 g         1613 t
ORIGIN      
        1 aaacaatgtc accaccggca atccttacgc tcaggaacag tgttggaaaa cagcaaatta
       61 ccttttatgt attggtatgc tgccatgcat ctgcttacca gcaccaaaaa atcgtactct
      121 gctgcagaac tgcaacgaca attaggccat aaacgctatc agcctatttg ggagatggta
      181 tgcaaattac gcgatgtgat ggggaaacgc tataatttgt accaattaac cgatgaggta
      241 gaactcgaca atgcttttat cgctacatta attgcggagg aagaaaagga taaacctctc
      301 aagcggggca taggaagcca aagacaaccc aaagcagtgg ttgtgaacga aagttccttc
      361 gtggaaaatc ccaaaccggg caaaaagccc aaacgtgtca atcatctaaa aatgttggtt
      421 atcgaagatt taagggctga taccatttca aaaactgtca atgaacacgt aaatccaaag
      481 gcggaattga ccaccaatga tgcaactcct tacagtaagt tgttcgaaca ggtcaaatca
      541 catcatgcgc aggctataaa gaaagaagac ctgccaaaaa cattaccatg ggtaaacatt
      601 gccattggca acgttaaacg attgttgttg gatgtacacc atcagcttac taatgaatac
      661 ttacaatact atctcaatga gttctgctat aaatttaacc gacgttattt tggtgatgcc
      721 ttcttcgacg ggtttcttat tgctgctgta agctacaaca ccgattttag gtcaagaatt
      781 tacaatagga attcatgcgg ataatcattt aaaattttta agcaatgcga aaaattggtc
      841 tttatatcag cgttttactt tcaattttaa attacgcttg caccgaccta atggagtata
      901 gcccctacga gggaaaggtt caggaaggct caagaagttt aaaccaaaca gcaattggta
      961 aacttcagtt gcaaagctct aaaacattta agccctttac atttgccatt ataggcgaca
     1021 gccataccta ttacgatgat tttatcaagc aaatatcggt gctcaataac ctcgatagca
     1081 ttgacatggt aatacacatg ggagatatca ccctaagtgg tatttataga gaatttctgt
     1141 ggtacaggga tataatcaaa ccgctgaaat atccattaat cacaattatt ggcaatcacg
     1201 attacctatc gaatggcgaa tacatgtatc gcgacatgtt tggccccacc aacttttcgt
     1261 ttacctataa caattgcctg cttgtgtttt ttgatgatat cacctgggaa cgtaatacta
     1321 aagaccctga ctttgaatgg ttaaaatcaa ccatctcagc tacagcaacc tacacccata
     1381 aatttgtgtt tacacacatc cctccgtggg atgaacaatt tagccatggt aatgagctat
     1441 attacaacta cctgctcgca aaaaacaatg tcgattactc aatacacgga catattcatc
     1501 ggttttacca tgacaagcat tacgctgatc tttatggcaa tgtaaattat cttgcctgtg
     1561 gcgatagcga ggaccatgaa gttgttctgg ttaaggtaaa ggaaaacaac atagatgttg
     1621 aagtaatcca cttttagcca tgaaaacgaa tagattttta cttttgggat tattgatttt
     1681 gctttatcat ccaaggctac atgctgaaga cccagtaaaa gggctaaggc aaaagattac
     1741 cgaggagaaa aagatagtgc ttaaaaacga tactgtaacc ataaagccat actggtttat
     1801 gccaacgcaa ttcaaagttc agtatgcagg gagtataggt tttgtatctt ttggtgcggg
     1861 ttacgatttc cgtaatggat atcaaccaac tttactatat ggtttcctcg ataataactt
     1921 tggagggagc aacataaccg ttcacaccat atctcttaaa aatcgattta agcttacaca
     1981 aaatccaata cttggatgtt ttacacctat tgcgggaata tcggtaaact ggggcataac
     2041 caataatacg tttaaccgct tgccaccaca ttacccggag aactactatt ttcagaataa
     2101 aatccattta tctccttttt ggggaggtga atttaatttt tcggtagctc aaaaaaactt
     2161 gggcgtttac tttgagttct cgacccttga tgcttacctt ttggaatatt ttagaaccga
     2221 ttttgtaaaa ctgaacaaaa tttggagttt aggcatgggt ataacgcttt acctaaacta
     2281 aaaacttcag caaaaagaaa agggataaaa cccacataac caagctaacc tgttttacct
     2341 ttccggtaaa tgccttaagc aaaacatacg atagcatgcc aaatacaatt ccctcggcaa
     2401 tgctataggt taatggcatc attataattg taaaaaaggc aggaattgct tcggttacat
     2461 cattaaaatc aattttaagt atagggctta gcataaaaga acccacaaga attaatgcag
     2521 gagcagttgc tgctgcagga accattaaaa acatgggcga aaagaaaagt gcagcaataa
     2581 aaagcatggc tgtagtaaat gaagtcaaac cagtccgacc accttcagca acccccgatg
     2641 cgctctcaac ataggtagta acagtgcttg tgccaagcat ggccccaaga gttgtgccaa
     2701 tggcatcggc aaacagggct tgcctagcac ggggtagttt gcctttttca tctaacatgt
     2761 ttgcttttgt ggctacgcca actagggttc ctaaagtatc gaacatgtca acgaataaaa
     2821 aagtaaaaag cactatcagc atatcgatac tcaaaatagt atgaaactca aacttgaatg
     2881 ctattggttc aactgaaggt ggcatactaa ataatccgcc ttggggtaat tcagttaaac
     2941 caaagggaat gcctgcaagg gtaaccaaaa aaatcccgat aagcaaagcc cctttaacct
     3001 tgcgagccag taaaacaccg gttacaatca caccggcaag gcacaacaaa accgatgggg
     3061 aacccatttg ccccaatccc accatcacgt tatcgttctt tacaatcacc cctgcattct
     3121 gaaggccaat aaaggctata aacaacccta tgccagccga tatggcatgt ttaattccaa
     3181 gagggatagc atcaattatt ttctcccgga tatttaagaa ggttaatgca atgaatatca
     3241 acccttccag gaatacggct gttaaagcaa actgccacga gtgccccatt ccaagcacta
     3301 ccgtaaaggc aaaaaaggca ttaagtccca ttcctggcgc cagcgcaaaa ggcagccttg
     3361 cccaaattgc cattatcatg gttgccaaaa acgatgccag ggctgttgtg gtaaaaagtg
     3421 catcgcggct cattccggta ttcgacaata tagaaggatt aaccgcaaga atgtaggcca
     3481 tggtcatgaa agtggttata ccagctagaa cctcggttct tattgacgtg ttagccttac
     3541 tgagttcaaa ataattatca atccgcttaa tcattggttt ttggaacaac tgattctttc
     3601 gcctgttgat tgaaaaggtc attcaaatat caaacccaat acaaggtgta taaattcagt
     3661 agattattta tcaacgcccc ccatggtaac tgatagtatg gttgtatggt cgtattcctg
     3721 accgggtagc aaaagcgttg tagggaacct accctcgtta ggtgagttgg ggaagtgttg
     3781 gggttccatg cataccgccc agttaccttt ttcgggtagt tttatcatgg ggttggcaat
     3841 atttggctta ttcatagggg tgtaaagctg aatgccgggc tgtgtggtgt aaatggttaa
     3901 ccctatatgg gttttggggt tgaacagcac caccgatgga ttgtcaattg tggggttgtt
     3961 cagcatgtag caatggtcgt aacatgttcc caccaggtcg gtatcggccc caacggcctt
     4021 aaacttccgg aaatcgaagg gtgaaccatc aacctctcgg atttcgccgg ttggaataag
     4081 ctcgctgtta accggcaggt aatggttggc atcgatgcgc agcatgtggt caagtacatc
     4141 ggtttcaaac ccgctcaggt taaaatatcc gtgagtggta aggttcaggt gggtaggcct
     4201 atcggtaaca gcacggtagc ttatgctcac ctgattatca tcggtaatct ggtaaattac
     4261 ccaaacatca acgtttcccg gaaatccttg gtcgccatgc aggctgttta ggtaaaactc
     4321 cagcatgcac accccattat gctcctcaaa cattacggtt tcccaaactc tatggctaaa
     4381 acctattttc ccgccgtgca ggatgttttt accctcatta gcgcatagcg catactcatc
     4441 ggaaccaatg gtaaatttgg cgttagcaat acgattggca taccgcccaa caattacccc
     4501 agggtagtaa tcggccttca ggtagtccga tgggcgtaaa agcccataag ccaggttaag
     4561 cggttcgttc tcatcgttca ggaggcgcat ggccaccatg gcagcaccca ggttcgatat
     4621 ggatacctcc aatcccccct cggtggatat ggagtatttc attacatcct taccatctat
     4681 gctaccccag tggctggcaa aaaagttcat aggctacaaa tcggtttcgt caacaataaa
     4741 aatatcctcg tttggttttt tcataagata ctgttcccgc gcgaattttt cgaggttttc
     4801 ggcattggtg cgtaactcct taattttctt tttatcctct tcaatctttt gcaggtagta
     4861 aactttttct tcctcaaggc tctttatacg gcgctttgcg gcaagcattt ctacccagtt
     4921 gctactatca aaaatcagca tccagattaa aaaaataaaa actgtcagga aaaacttatt
     4981 cctaagtttg ggcaaaaacc atttccagaa agtaatttcg ctaaccggca tgtcaacctc
     5041 cactaatcag gtgaataacc tgcacatcat cgccatcctt gatggttgcc atgctatact
     5101 cgtccttttt aaccagttta ccattcactt taaccacaag cattttaaag gagaaatgct
     5161 taaaggctag cagctcgtta acggtaatag cagccttatt ggtatctatg tgctccggca
     5221 aattgttaag tgtaatgtcc atagcaaaaa gtatttgggc gtgccccctg aaggggtcgg
     5281 gctactccgg gcttcgcttc gctccggcaa tacccgcccc ctggcgggta tgtgctgctg
     5341 cgcaccctcc gtatccctca cgcaaatata accataacgc actgcaaaat cataacatca
     5401 accaaagatt tttgggattt caaccccggg aaaaaacatc gttttgttct ctcctgtcat
     5461 gctgagctag ccgaagcatc tctttgtacc ttctctacct taatgtcttt tatagttctt
     5521 tgtcatgctt tcccgttgcg atgcaatgct tcccccttgc tctttggaaa aatattgtgt
     5581 gcatcgcttt acgtgtggga gttgtcatag ggttacaaac attatgcccc tacggggctg
     5641 gaagcgttgt tcgtggaatt gacctcaccc cagcccctct cctgtcagga gaggggaggg
     5701 ccgttaaagt ctcccctctc aggggagatt tagaggggtc atctaggttg aatgtggaaa
     5761 acagtctcac cacagtcctt taccctgaga aggaatggag cggaaaaagc aagcagtggc
     5821 ataaaagcca gacccggcaa tgggtcgggg gtggaacgtg gcagtgggct tagtaagcga
     5881 taccacatga gtcacgccgc aggcgtggcg aatagtat
//
LOCUS       DAOOWJ010000136         5775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_14045_length_5775_cov_7.895804, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000136 DAOOWJ010000000
VERSION     DAOOWJ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14045_length_5775_cov_7.895804"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            535..1416
                     /locus_tag="PLW22_10630"
     CDS             535..1416
                     /locus_tag="PLW22_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75197.1"
                     /translation="MFYLLLCILSSTVVLITFKLLNRFSVNNFSAIIINYLAASVAGF
                     FLAKGELSGFYQLGILPILLMVAMGVLFITMFHFLGKSTQTAGVAITSVAAKMSVIIP
                     ILVSVLIDPNDKLNAVRLAGILLALIAVLLLVIPRKTEKFKPVKTYLPLIIFLGIGMV
                     DASVKVAQQYFVTNELNPLFNAVVFAMAGITGLLLLPFNTHAAKDFHKFETWLLGTLL
                     GLANFGSMFFMVAALNHVGSKGTPLEGSVLFGINNLGVVLAGTLVGLLLFGERPSKTN
                     LSGIALSVLAIVILMQS"
     gene            1417..2034
                     /locus_tag="PLW22_10635"
     CDS             1417..2034
                     /locus_tag="PLW22_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOU75198.1"
                     /translation="MVEVADTYKTIDSPAEGIYKEKGSKFLAKAYPVRTEEQVKAIVE
                     QLKEEYYDARHHCYAYILGPKGDKWRANDDGEPSGTAGKPIHGQLLSFNLTNVLVVVI
                     RYFGGTKLGVSGLINAYKTATHDALTNAKIITRTVDAIYSVTFGYTMMNEVMRLAKDL
                     NLQLLEQQFDNTCIIRVRIRRSLEGEFLSRCSKIEGLIADFEFED"
     gene            2098..3009
                     /gene="pyrB"
                     /locus_tag="PLW22_10640"
     CDS             2098..3009
                     /gene="pyrB"
                     /locus_tag="PLW22_10640"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOU75199.1"
                     /translation="MKNRSLVAINDLSRDEIFRVLDLAEEFEKNPIQRIMEGKVVATL
                     FFEPSTRTRLSFESAVNKLGGRVIGFSEASTSSVSKGETLKDTILTVANYSDLIVMRH
                     PVEGSARYASEVSPVPIVNAGDGANQHPTQTLLDLYSIRKTQGKLDNLKIVMVGDLKY
                     GRTVHSLLMAMSQFNASFTFISPAELRMPLEYKMYLDELGLKYEEHTDLAENIKDADI
                     VYMTRVQRERFSDPMEYEKVKNAYILKNYMLEGTKPNMRVLHPLPRVNEINTDVDESP
                     KAYYFTQALNGVYTRMAIMALILGLKK"
     gene            3022..3480
                     /gene="pyrI"
                     /locus_tag="PLW22_10645"
     CDS             3022..3480
                     /gene="pyrI"
                     /locus_tag="PLW22_10645"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOU75200.1"
                     /translation="MKDKKHLSVSAIENGTVIDHIPAQNLFKVITILGLDRIPNQITF
                     GTNLESKLLGKKAIVKIADKYFERDEINRIALVAPHAKLNIIKNYEVTEKMLVEVPND
                     ISGIVKCVNPKCITNVENVTPKFKVVDKNSVSLKCHYCEKITDQEHMEYL"
     gene            complement(3529..3951)
                     /locus_tag="PLW22_10650"
     CDS             complement(3529..3951)
                     /locus_tag="PLW22_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU75201.1"
                     /translation="MLRRRSTLPSLVDEFFGDDFISRFFDDSESVTIPSVNIKEGKDD
                     YTIEVAAPGFDKKDFKVDLNNNVLEISSEKEVKEENKDEKVMRREFRYSSFKRSFTLP
                     DTADTDKIKASYKDGILSIIIPKKEEAKVKPVRQISIS"
     gene            complement(4357..4779)
                     /locus_tag="PLW22_10655"
     CDS             complement(4357..4779)
                     /locus_tag="PLW22_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75202.1"
                     /translation="MKAYKLYLFAVIVSFLNSCGDKDVCEDYRFENATEHRVYIRSCC
                     GEATDSNTSLSIGPKSSATLTSCALGYSDFLYNRLSGCVVDRVLIIFDDTLELNTRDF
                     GEFDLLNWDNYQRKNKEYIYEINQLHYQKALEVNGYLR"
     gene            complement(4912..5352)
                     /locus_tag="PLW22_10660"
     CDS             complement(4912..5352)
                     /locus_tag="PLW22_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75203.1"
                     /translation="MKPIKIIYKLLISIGVIMFFSYASCDKWVCGDWWFINLTEHKIS
                     VDVYCFGSISTELSFSLMPNDSLKQITCDIGSSPPPFVSSTCRIDSILLVFDDTLVLN
                     TLNYWEFNPENIEKWENYNRKSNKKYNFTESHYQKALEVNGYSR"
     gene            complement(5357..>5775)
                     /locus_tag="PLW22_10665"
     CDS             complement(5357..>5775)
                     /locus_tag="PLW22_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75204.1"
                     /translation="FALIWLNPKTRIIPESWAVAVEWGLTNTEYHILGQKYGSYKALS
                     YNFKEGKQFWYRGNDEFYTPLFIDLIDDQNQRINNNGSILFPNDKVKGYSLSILESIL
                     FGVRDLELLKAMLKINKPFGVANEDIDELINFYKNI"
BASE COUNT         1743 a         1220 c         1119 g         1693 t
ORIGIN      
        1 agccttgatt ttctttggtt ctttcttgta tcaagacaag aaagaacgta aaaatagttc
       61 tttgccatgc ttctcggttg cgatgcaacg cttccccctt gctctttgga atactatagc
      121 gtgcatcgct ttagagtaga gatagcaatg aagttacaaa tgtttcgccc ctatggggct
      181 agaaacgttg tttgttgctc gtgaaactat tgtcatgttg agcgatagcg aaacatctct
      241 ttagggccga taagagctcc ttcgctgcgc tcaggatgac aaggagttga cagttgatgg
      301 ttgatagttg atagttaaaa aaagcatttc acgaaacact aaacaccaaa cactaaacac
      361 taaaaacccc tgtacactga aaactatcca aaagatcctt cgctgcgctc aggatgacaa
      421 attacggttg tactcgctta aactacattg catgttaatt gtatgttaat acttacctct
      481 aaaaaaattt gtggggtaag taaagatgct ataactttgc acaaacctaa aaggatgttc
      541 tacttactgc tctgcatatt atcatctaca gtagttttaa tcacctttaa gttactcaac
      601 cgcttttcag tcaacaactt tagtgcaatc atcattaact atttggcagc cagcgtagct
      661 ggtttttttt tggccaaagg tgaactatcg gggttttatc aactaggcat actacccata
      721 ctactgatgg tggctatggg tgtactcttt ataaccatgt tccatttcct gggtaagagt
      781 acgcaaacag ccggtgttgc cataacgtcg gtagcagcta agatgtcggt aataatcccc
      841 atactggtat cggtactgat tgacccaaac gataagttaa atgcagtacg attagcaggc
      901 attttgcttg ccttaattgc agttctctta cttgtaatac caagaaaaac ggaaaaattt
      961 aaaccggtta aaacatatct tccactaata atctttttgg gtattggaat ggtagatgcg
     1021 tcggttaagg ttgcacagca atactttgta accaacgaac taaatccctt gttcaatgcg
     1081 gttgtatttg ccatggctgg cattacaggc ttactactgc taccttttaa tacccatgca
     1141 gccaaagact ttcataaatt cgaaacatgg ctactaggaa cattgcttgg attagcaaat
     1201 tttggatcga tgttttttat ggttgctgca ctgaaccatg ttggaagcaa aggaactccc
     1261 ttggagggat cggttctttt cggcataaac aatttggggg tagtactggc aggaacgctg
     1321 gttgggctgc tactatttgg tgaaaggcca agcaaaacaa atctttccgg gatagccctt
     1381 tctgtattgg caattgtaat acttatgcaa agctaaatgg ttgaagtagc tgacacatac
     1441 aaaaccatcg attcgcctgc cgagggaatt tacaaggaaa aaggcagtaa gttcctcgct
     1501 aaagcctacc ctgtaagaac cgaggagcaa gtaaaagcaa tagttgagca acttaaggaa
     1561 gaatactatg atgcccggca tcactgctac gcatatattt tagggccaaa gggcgacaaa
     1621 tggagggcta acgacgacgg cgaaccatcg ggtactgccg gcaagcccat tcacgggcaa
     1681 ctgctcagct ttaacctaac caacgtttta gtggtggtaa tacgttactt tgggggaaca
     1741 aagctaggag tatcgggctt gataaatgca tataaaacgg caacccacga tgccttaacc
     1801 aatgcaaaaa ttatcacccg aactgttgat gccatttaca gtgtaacctt tggttacacc
     1861 atgatgaatg aggtgatgcg attagccaag gatttaaacc ttcaactact agagcagcaa
     1921 tttgataata cctgcattat cagggtaagg attaggcgct cactggaagg cgagttcctt
     1981 tccagatgct ccaagattga aggacttatt gccgattttg aatttgagga ttgatgtact
     2041 agccccgttt atcggggctt ttttttgaac catatactta aacacaaaat aaaaactatg
     2101 aaaaacagaa gtttggtagc aatcaacgat ctgagcaggg atgagatatt cagagtgctc
     2161 gaccttgccg aggagtttga gaaaaaccct attcagcgaa tcatggaagg gaaagtggtg
     2221 gcaacacttt tctttgaacc gtcgacccga acccgcttga gctttgaaag cgctgtaaac
     2281 aagctaggtg gacgggtaat tggcttttcg gaggcatcaa ccagtagtgt gtccaaaggc
     2341 gaaaccctta aggatactat tctcaccgta gccaactatt ccgacctgat agtgatgcga
     2401 caccccgtgg agggtagcgc acgctatgca agcgaggtat cgcctgttcc cattgttaat
     2461 gcaggcgatg gtgccaacca gcatccaacc caaacccttc tcgaccttta ctccattcgc
     2521 aaaacgcagg gtaaactcga taacctgaag attgtaatgg taggcgacct taagtatggc
     2581 cgaaccgttc actccctgtt gatggccatg agccagttta acgcctcatt tacatttatt
     2641 tcgcccgccg aactccgaat gccactagag tataaaatgt atctcgatga gttaggctta
     2701 aagtacgagg agcataccga tttggccgaa aacattaagg atgccgatat tgtttacatg
     2761 acccgggttc agcgcgaacg cttctccgac cccatggaat acgaaaaggt taaaaacgcc
     2821 tacatcctga aaaactatat gcttgaaggt acaaagccaa acatgcgagt gctacatccg
     2881 ctacccaggg taaacgagat aaatactgat gtagatgaat cgccaaaggc atactacttt
     2941 acccaggcac taaacggcgt ttacacccgt atggcgatca tggctcttat tttaggtcta
     3001 aaaaagtaaa agaatatcga tatgaaagac aagaaacacc tgagtgttag cgccattgag
     3061 aatggtacag taattgacca tataccggcg caaaacctgt ttaaggttat taccatcctc
     3121 ggactcgata ggataccaaa ccaaataacc tttggaacaa acctggagag taagctactc
     3181 ggcaaaaagg ctattgttaa gattgccgat aagtactttg agcgcgatga gattaaccga
     3241 atagcccttg ttgctcctca tgccaagctc aacatcatta aaaactatga ggtaacggag
     3301 aaaatgctgg ttgaagtacc caacgatata agtggcattg taaaatgcgt aaacccaaag
     3361 tgtatcacca acgttgaaaa tgttacccct aaattcaagg tggttgacaa gaatagcgtg
     3421 tcgttaaagt gccactactg cgaaaaaatt accgaccagg aacacatgga gtacctataa
     3481 acagtaaagc cccgtttccg gggcttcgct gtttttaaca tcttcccgtt acgatatcga
     3541 gatctgacgt acgggtttaa ccttagcttc ttccttcttt gggatgataa tgcttaagat
     3601 gccgtccttg tacgaagctt taatcttatc ggtgtcggct gtatcgggta aggtgaacga
     3661 ccttttgaat gaggagtaac ggaactcacg acgcattacc ttttcgtcct tattctcctc
     3721 cttaacctct ttctcgctgg aaatctccag cacgttgttg ttcaagtcaa ccttaaagtc
     3781 cttcttgtcg aacccgggag cagccacctc aatggtgtaa tcgtcctttc cctccttgat
     3841 gtttaccgaa gggattgtta cactctcgct gtcgtcaaag aaacggctga taaaatcatc
     3901 cccaaagaac tcatctacca gacttggtag agtgcttctg cgacgtaaca ttggcatcat
     3961 agttttaccc tcccattatt ttggttagac atcaatgctt tcacctagca tttttcaaac
     4021 cgtatgccaa ccctttattt gtgtcaattt ttcataaaac tagtaaaaca aaggtcaatt
     4081 tgtcacagaa aagtataaaa ttgactcaaa ctcaatcaat atgaattagg tgtgattaaa
     4141 tggcttgatt taaatttcat tgattttgtt tgtaaacgca atttcaatgt cgtttagtta
     4201 ggtatagttg ccctgttgat tcctgacatt acgggataaa ctccacataa acatcacact
     4261 gtcatgctga acgctgaagc atcttttttg ctctttttta tttcacactt gcctgagata
     4321 tttcactctt agccgtttac cggcagggag accgttttac cttaagtacc cgttaacctc
     4381 aagcgctttt tggtagtgca gttgatttat ttcgtaaata tattccttat tcttgcgctg
     4441 ataattatcc cagtttagca agtcaaactc cccaaaatcc cgtgtattaa gttctagagt
     4501 atcatcaaaa ataatgagca ctctatccac tacacagccg cttaatctat tatataaaaa
     4561 atcagaatat cctaaagcac aactggttaa tgtagcgcta ctcttagggc caatgctaag
     4621 ggatgtattg ctatcggttg cttccccgca gcaggaccgg atatacaccc tatgttccgt
     4681 ggcattttca aacctgtaat cttcacatac atccttatct ccacaagaat ttaggaagga
     4741 tacaattaca gcaaataggt ataatttata tgctttcatc gcttttaaaa tttaaaaaaa
     4801 gtatgcattt tttttattag taagaccctt agagctttta gtaataatat tggcactgaa
     4861 ctattcaaat tgtgttaaag tccccctccg ttaccggcag ggagaccgtt tttaccttga
     4921 gtacccgttc acctcaagcg ctttttggta gtgcgactcg gtaaagttat actttttatt
     4981 acttttcctg ttgtaatttt cccatttctc aatgttctct ggattgaatt cccaataatt
     5041 taaggtattt aatactagag tatcatcaaa aactaacagt attgaatcta ttctgcaagt
     5101 actcgaaacg aatggcggtg gtgaagaccc tatgtcacat gttatttgtt ttagggaatc
     5161 attaggcatt aatgagaatg ataattcagt actgatactg ccaaaacaat atacatcaac
     5221 agatatttta tgttctgtca aatttataaa ccaccaatcg ccacaaaccc acttatcaca
     5281 actggcatat gaaaaaaaca taattacccc tattgatatt aataatttgt aaattatttt
     5341 aatcggcttc ataggtttaa atatttttat agaaattaat caattcatca atatcctcat
     5401 tagcaacacc aaaaggcttg tttattttta gcatggcttt aagtaattca agatctctta
     5461 caccaaaaag aatactctca agaatcgaaa gggaataacc cttaacttta tcattcggaa
     5521 ataaaataga gccattatta ttaatgcgtt gattttggtc atctatcaaa tcaataaata
     5581 gcggtgtata aaattcatca ttacccctat accaaaattg tttgccttct ttaaaattat
     5641 aactcagagc cttataactc ccatactttt gccccagtat gtgatattcc gtattggtaa
     5701 gtccccattc tacagcaacc gcccaactct ccgggattat tctggttttt ggattaagcc
     5761 atataagggc aaatg
//
LOCUS       DAOOWJ010000137         5744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_14149_length_5744_cov_6.893479, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000137 DAOOWJ010000000
VERSION     DAOOWJ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14149_length_5744_cov_6.893479"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PLW22_10670"
     CDS             complement(<1..895)
                     /locus_tag="PLW22_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOU75205.1"
                     /translation="METSWFVVVNPRAGGGKSVVDWPIISRLLNKYDIHYSHAFTEYK
                     YHAVELTVQAIVKGYRNIIAVGGDGTLNEVVNGIFIQKTVSPSDITVGVIGDGTGNDW
                     FRMYEIPHNYEECVRAIKNKRVFKQDVGVAEYYESSVRHTRYFINAAGVGFDAEVARR
                     TNRLKDLGKRGALLYILSLIKALIRYRSTRINVKVDGKHFQHTVFSLTIGICRFNGAG
                     MMQVPFALSDDGLFDVTIIKKISKLNVVANLPRLYNGTILKHSRVLGVRGKSIEIMSI
                     PAVGLEVDGESLGETPLKFSIM"
     gene            1139..1939
                     /locus_tag="PLW22_10675"
     CDS             1139..1939
                     /locus_tag="PLW22_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU75206.1"
                     /translation="MAKVIALANQKGGVGKTTTAINLAASLAVLEKKVLLIDADPQAN
                     ATSGTGFDLRNVKTSIYECLIDDINPKDIVLNSEIKGLDLIPSHIDLVGAEIEMLNMP
                     NREKILKGVIEKVKDDYDFVFIDCSPSLGLITVNALTAADSVIIPVQCEYFALEGLGK
                     LLNTVKIIQTRLNTELQIEGFLLTMYDPRLRLSNQVVDEVRKHFQQMVFETIIQRNIK
                     LSEAPSYGKPVILYDAASTGSLNYMNLARELLQKNNMTSIDNSSKIEE"
     gene            1959..2870
                     /locus_tag="PLW22_10680"
     CDS             1959..2870
                     /locus_tag="PLW22_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU75207.1"
                     /translation="MSKKMALGKGLGALIDDAVDPTRNQDIKTEKQVVSELVNEIEIE
                     KIDVNPYQPRTQFDEEALKELAESISKLGVIQPLTVRAVDGRFQLISGERRLRAAKLA
                     GLKTIPVFIRTADDQGMLEMALVENIQREDLNAIEVAISYQRLIEECNLTQETLSERV
                     GKKRATISNYLRLLKLPAEIQLGISQDLISMGHARALITIEDPKVQLDIYHQTIAEGL
                     SVRRVEELARMANDPTPVSPAKTKDGKEKVNLGEQEIVLKDHLTNRLGLRADVKPGPK
                     GDGKIVISYNNQAELEAILEKFIKIEE"
     assembly_gap    2872..2971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3104..3952
                     /locus_tag="PLW22_10685"
     CDS             3104..3952
                     /locus_tag="PLW22_10685"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOU75208.1"
                     /translation="MIFLFLLIAPAAYTQTNNPKGLKKPDITTRIWLGSHVLPGYGQV
                     YNRDYWKIPIFYAGMGTMAYQGYQMNSDYKKWQKTYLALPTSDPDREKFKIKATEYRQ
                     NRNLYYAAATAFYLASVSDAVLVYNRGKHSPAAASILSTLVPGMGQAYNGAYWKIPII
                     YGGLSTFYFIIDWNNRGYNRFRTALFNLIDGDPATVDEFNGTRTQEELRYYMDNYRRN
                     RDLSALGFAVVYALNIIDANVDGHLYDWNVDDNLTFRVEPAVFNPSQNLTYNTPAFGV
                     SCRVTF"
     gene            4285..4419
                     /locus_tag="PLW22_10690"
     CDS             4285..4419
                     /locus_tag="PLW22_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75209.1"
                     /translation="MVNGKIERIEKFTSKYVTPRNIDIWLPSGYSDSTKYAVLYMHDK
                     "
     gene            4420..4704
                     /locus_tag="PLW22_10695"
     CDS             4420..4704
                     /locus_tag="PLW22_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75210.1"
                     /translation="MLYDPSTTWNKQAWDVDNAVSVLMAENRVKNFIVVGIWNGGKTQ
                     HQDYFPQKPFEKLSQAEKDTITARLKRVGRTEGAFQPQSDNYLKIYCKRT"
     gene            complement(4968..>5744)
                     /locus_tag="PLW22_10700"
     CDS             complement(4968..>5744)
                     /locus_tag="PLW22_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOU75211.1"
                     /translation="DIFLTETARFAHVVLPASAWAEKNGTFVNSDRRVLRVRKAVDCP
                     GQAREDWRIIHELAARMGKPMPNYRDEAEIFDELARVTPIMAGISHARLEHHGIQWPC
                     PTPDHPGTPTLYLDKFNTPSGKAKLFPVEHVDQTERVTTEYPFILNSGRILYHYHTGT
                     MSRRNKALTNFTNNPYVIMHPNDASKLGIKHGEKVRITSVRGTLETQVRIADEVLEGE
                     LFMPWHYTEAQVNRLTRDELDPYSRIAPFKISAVRVEPAS"
BASE COUNT         1681 a         1319 c         1227 g         1417 t
ORIGIN      
        1 gcataattga gaatttaaga ggagtttcgc caagcgattc gccatcaacc tcaagaccta
       61 cggctggaat actcattatc tcaatgcttt tccccctcac gcctaaaaca cgtgaatgct
      121 ttaggatggt tccgttgtaa agccgtggca ggttggcaac cacattaagc ttgcttatct
      181 ttttaataat ggtaacatca aacagcccat cgtccgaaag ggcaaaagga acctgcatca
      241 ttccagcgcc gttaaagcgg caaattccaa tggtgaggct gaaaactgta tgctgaaagt
      301 gcttaccatc aaccttaaca tttattcggg ttgagcggta ccggataagc gccttaataa
      361 ggctaagaat gtatagcaac gcgccacgtt tccccaaatc ctttaaacgg ttagtccggc
      421 gagccacctc ggcatcgaaa ccaacgccag cggcgttaat gaagtaacgg gtatgcctaa
      481 cgctcgattc gtagtactcg gccacaccta catcctgctt gaataccctt ttattcttta
      541 ttgccctaac gcattcctcg tagttgtgtg gaatctcata catccgaaac caatcgtttc
      601 cagtaccatc acctattacc ccaacagtaa tatcgctggg gcttaccgtc ttttgaataa
      661 aaatcccgtt aaccacctcg ttcagggtac catcgccacc aactgcaata atatttcggt
      721 agccctttac tattgcctgc acggtgagct caaccgcatg gtacttatac tccgtaaagg
      781 catgggaata gtgaatatcg tacttgttga gcaaacgact aatgataggc caatcgacaa
      841 ccgatttacc ccctccagcc cgcgggttta ccactacaaa ccaacttgtt tccaacgtat
      901 agaaatttag tcatcgaatc cgatgcaaat ataaccattc tgttaaaatt cattcactta
      961 tccggaaaat ggtttttgtt tgataaaaga atttctattt ttgcagttac cgtttgcaca
     1021 aaccgattgt taataacttg ttaataacat cggggcaaat gttcatattc acatccatgg
     1081 gggtgtggaa tgaaaaggaa aggaaactta tttttgaccc ttgtaaaaaa acatagtaat
     1141 ggcaaaagta attgcattag ctaaccagaa aggtggtgta ggtaaaacca ccacagctat
     1201 taaccttgca gccagcttgg cggttcttga gaaaaaggta ctcctgattg atgccgaccc
     1261 tcaggccaac gccacatcgg gtaccggttt tgatttgcgc aacgtaaaaa ccagtatcta
     1321 cgagtgcctc atagatgata tcaacccaaa ggatatcgtt ttaaactctg aaattaaggg
     1381 attagaccta attccatcgc acatcgacct ggtgggtgcc gagatcgaaa tgcttaacat
     1441 gcccaacagg gaaaagatac tcaaaggagt aatagaaaaa gtaaaggatg attacgattt
     1501 tgtattcatc gactgttcac cctcattggg cttaattacc gtaaacgcac ttactgctgc
     1561 cgattcagtt atcatccccg tacagtgcga gtactttgcg cttgaagggt tgggcaaact
     1621 actcaatacc gttaagataa tacaaacccg cctcaacacc gagctgcaaa ttgaaggatt
     1681 cctgctcacc atgtacgacc caaggctacg cctttcgaac caggtagttg atgaggtgcg
     1741 taagcacttc cagcaaatgg tattcgaaac catcatccag cgcaatatca agctgagcga
     1801 agctcccagt tacggtaaac cggttattct ttacgatgcc gcctcaaccg gatcgttaaa
     1861 ctacatgaac ctggctcgcg agctgctgca gaaaaacaac atgaccagca ttgacaatag
     1921 tagcaagata gaggaataag aaggaggaaa tatttaccat gtcaaaaaag atggctttag
     1981 gcaaagggtt aggtgctctt attgacgatg cggttgaccc aacacgcaac caggatatta
     2041 aaaccgaaaa acaggttgtg agcgagctgg ttaacgagat tgaaattgaa aaaatcgacg
     2101 ttaaccccta ccaaccccgt acccagtttg atgaggaagc gctaaaggag ctagcggaat
     2161 caatatccaa acttggtgtt atacaacccc tcacagttcg tgcggttgat ggtcgctttc
     2221 agcttatttc gggtgagcgc cgcctaaggg cagcaaagct tgccgggcta aaaacaatcc
     2281 cagtattcat tcgcactgcc gacgaccagg gtatgcttga gatggccctg gttgaaaaca
     2341 tccagcgtga ggaccttaac gccattgagg tagccattag ctaccagcgc ctgattgagg
     2401 agtgtaacct tacccaggaa accctatccg agagggttgg caagaaaagg gctaccatat
     2461 ccaactactt gcgcctgctt aaacttccag ccgaaattca acttggcata agccaggacc
     2521 taattagcat ggggcatgct cgcgcactta ttaccattga agatcctaag gtacagctcg
     2581 atatttacca ccaaaccatt gccgaagggc tatcggttcg tagggttgaa gagctagccc
     2641 gcatggctaa cgatccaact cccgtaagtc ctgctaaaac taaggatgga aaggaaaagg
     2701 ttaaccttgg tgaacaggaa atagtcctga aggatcatct taccaaccgc ttgggactcc
     2761 gcgccgatgt taagcccggt cccaagggcg atggcaaaat tgttatttct tacaataacc
     2821 aggctgaact ggaagccatt cttgaaaagt ttatcaagat tgaggagtag annnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaccaggct gaactggaag ccattcttga
     3001 aaagtttatc aagattgagg agtagatact agtgttggaa tccatgcatg gcactattta
     3061 ctgtttaact ggccgacatg tcaacaaaaa cctgtcggcc tttgtgattt ttctattttt
     3121 actcattgct cctgcagcat acacccagac aaacaaccct aaaggactga aaaagcccga
     3181 tataaccacc cgtatttggc tgggctcgca tgttttacct ggttacggac aggtttacaa
     3241 cagggattat tggaaaattc ccattttcta cgcgggtatg ggcaccatgg cataccaggg
     3301 ctaccagatg aacagcgact acaagaaatg gcaaaagact taccttgccc ttccaacctc
     3361 cgacccagac cgcgaaaaat ttaaaataaa agcaaccgag taccggcaaa accgaaactt
     3421 atattacgcc gcagccacag ccttttacct agccagcgtt tccgatgccg ttttggttta
     3481 caaccgaggc aaacactccc ccgccgctgc atcaatactt tcaacactgg tgcctggcat
     3541 ggggcaagcc tataacgggg catactggaa aattcctatc atctacgggg ggttatccac
     3601 tttctacttt attattgact ggaacaacag ggggtacaac cgattcagaa cggctctttt
     3661 taatctgata gacggcgacc cagccacagt tgatgagttt aacggaacac gtactcagga
     3721 agaactccgc tactacatgg acaactaccg gcggaaccgt gacctttcgg ccttaggctt
     3781 tgcggtagtg tatgcgctga acatcattga tgccaacgtt gatggccacc tgtacgactg
     3841 gaatgtggac gacaacctga ccttcagggt tgaaccagcc gtttttaacc cctcgcaaaa
     3901 cctaacctat aacacacctg cctttggggt ttcatgtagg gttacgtttt gaataagtga
     3961 acaggttaat gaataacggt taaaggaggt tcttgaaccg taaaccatta aaaatcagac
     4021 gaataataag gagtacgaag aatttaaggg gttacttacc aacaaaaatt aaccaaacca
     4081 gttgaacata atcacactcc cataatgtta atataaacag cgggaaagtc caccatttgt
     4141 gtaaattata cccaaaaaca taaaatttaa agttaaaaag atgttcattt tttaggaatt
     4201 gataccatgc gcttgaaatt taaaataacc attatcattt tattgctatc tgcccaaatt
     4261 aaggcacaaa ctaatttgcc tcaagtggtg aacggtaaaa ttgaacgcat tgaaaaattc
     4321 acatcaaagt atgtaactcc acgaaatatt gacatttggc tcccaagcgg atattccgac
     4381 tcaactaaat atgcagtact ctacatgcac gataagtaaa tgctttacga tccctccact
     4441 acttggaaca agcaggcttg ggatgttgac aatgcggtat cggttttgat ggcagaaaac
     4501 agagttaaaa actttattgt ggtaggcatt tggaatggtg gtaaaaccca gcaccaggac
     4561 tacttcccac aaaaaccatt cgaaaagcta tcgcaagctg aaaaggacac cattactgct
     4621 cggctaaaaa gagtgggcag aaccgaaggt gcttttcaac ctcaatccga taattaccta
     4681 aaaatttatt gtaaaagaac ttaagcccca cattgataaa agatattctg tttacactaa
     4741 tcgggaaaat acctttattg caggtaatcc cgatagccat aaaatttatt ttgactatgg
     4801 cgattaaacc ctagacgcca tgtatccgga gattcagaaa agaattgata gcgttatggt
     4861 taataaaggg gtcaacagcc atagctggat aaccaggtat ttccctgggg aaaaccacag
     4921 tgaactggca tggagcaagc gtttacgtat ccccttggaa tttttactca gctcgccggc
     4981 tcaaccctaa cggccgaaat cttgaatggc gcaatgcgac tgtatgggtc aagttcatcg
     5041 cgggtaaggc ggttaacctg agcctcggtg taatgccagg gcataaagag ttctccctca
     5101 agcacctcat cggcgatgcg aacctgggtt tccagagtac ctcgcaccga ggtaatcctt
     5161 accttctcac catgtttaat ccccaatttt gatgcatcgt tgggatgcat aattacatag
     5221 ggattgttgg taaagtttgt tagagccttg tttctgcggc tcatggtacc agtatggtaa
     5281 tggtaaagaa tacggcccga gttcagtata aatgggtact cggtagtaac cctttcggtt
     5341 tgatcgacat gctccactgg gaaaagttta gccttgccgc tgggcgtgtt gaatttatcg
     5401 aggtataggg taggtgtacc gggatggtcg ggggtggggc aaggccactg gataccatga
     5461 tgttcaagtc gggcatgcga gatgccagcc ataattgggg ttaccctcgc cagttcatcg
     5521 aatatctcgg cttcatcgcg gtagttaggc atgggcttgc ccatgcgtgc agccaactcg
     5581 tgaataattc gccagtcctc gcgagcttgg cccgggcaat cgacagcctt acgtacacga
     5641 agcacacgcc tatcgctatt gacaaacgtt ccgttcttct cagcccacgc tgatgctggt
     5701 agcaccacat gtgcaaaacg ggcagtctca gtaaggaaaa tatc
//
LOCUS       DAOOWJ010000138         5736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_14175_length_5736_cov_7.045591, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000138 DAOOWJ010000000
VERSION     DAOOWJ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14175_length_5736_cov_7.045591"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLW22_10705"
     CDS             complement(<1..409)
                     /locus_tag="PLW22_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOU75212.1"
                     /translation="MQPIKVAIQGFKGAFHQDAAELYFNTPLELVECLTFKELVNSVT
                     EGRAEKGVMAIENSLVGSILQNYTLIRESDLTIEGEVFLRISQHLIGISGQRVEDIKE
                     IHSHPMAIAQCDDFIQSIPHVKVFATHDTALSAR"
     gene            833..1408
                     /locus_tag="PLW22_10710"
     CDS             833..1408
                     /locus_tag="PLW22_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOU75213.1"
                     /translation="MIKILLLDNYDSFTYNLYHYIDLTGGFIVDVIRNDALKLASINS
                     YDGIVLSPGPGLPKESGLLMNVIDNYHSKKKIFGVCLGHQALAEYFGAKLLNLPEVLH
                     GIQTPIKILEPKHYIFRDIPSTIEVGRYHSWVVNPIDLPDCLTVTAIDYDNQIMALCH
                     KKYDICSVQFHPESIMTPYGMKIILNWLDGF"
     gene            complement(1405..2055)
                     /gene="rpe"
                     /locus_tag="PLW22_10715"
     CDS             complement(1405..2055)
                     /gene="rpe"
                     /locus_tag="PLW22_10715"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOU75214.1"
                     /translation="MTRLVAPSMLSADFANLSCDIEMVNKSQADWFHLDIMDGVFVPN
                     ISFGFPVVERIKSLATKPLDVHLMIVDPDRYLERFREAGADWLTVHYEACTHLNRTLS
                     RIRELGMKAGVSVNPHTPVELLTDILEYADLVLIMSVNPGFGGQKFIPNSIGRIERLR
                     KLIDQKGLSTLIEVDGGVGPSNARELYQAGANVLVAGNAVFKADNPLAAIEMIKNA"
     gene            complement(2126..2989)
                     /locus_tag="PLW22_10720"
     CDS             complement(2126..2989)
                     /locus_tag="PLW22_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU75215.1"
                     /translation="MRQLKIIKQVTNREAVSLDKYLHEIGKVDLLTAEQEVTLARKIK
                     EGDQEALAKLVKANLRFVVSVAKQYQNQGLSLPDLINEGNVGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIVKLPLNKIGSINKVNRALAELEQRFEREPTIDELSE
                     MLNLAPDDVKEALKGNNRHLSMDAPITQDEEGSMYDVLLSPDSPMPDKGLLNESLRKE
                     IERALSTLSPREANIIRLYFGLNGKHPLTLEEIGEEFDLTRERVRQIKEKALKRLKQT
                     TRSKILKSYLG"
     gene            3188..4177
                     /locus_tag="PLW22_10725"
     CDS             3188..4177
                     /locus_tag="PLW22_10725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU75216.1"
                     /translation="MHFSVIIPVYNRPGEVEELLQSLAGQGYNSFEVIIVEDGSTVPC
                     SDIVNKFASELKINYYFKENTGPGLTRNYGAERAKGEYLIFFDSDCIIPNNYFTRVEE
                     ELAAEYVDFFGGPDATHPSFTNVQKAIGYSMTSFFTTGGIRGGRVKLERFTPRSFNMG
                     INAEVFHSVGGFSNMRFGEDVDLSLRLFEKGFTSRLFPKAFVYHKRRTDFRKFFKQVF
                     NSGMARINLSKNHKGSLKMVHLLPSGFVLVMSAAILLSWAFPIILTLPLLYTFAIMFD
                     SLLKGNSLTVAILSIVAAWVQLYGYGLGFIYATWLWVVHGKSPNGAFTKNFYR"
     gene            complement(4178..4846)
                     /locus_tag="PLW22_10730"
     CDS             complement(4178..4846)
                     /locus_tag="PLW22_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU75217.1"
                     /translation="MNKVIALSPIFRGLTENEVAHLLNETPHTIRSFSKGQTIAQRTE
                     EVRNLCILVEGSVKGEMVDFSGKILKIEEMHAPMPIAHAFLFGENNRYPVDVIALENC
                     KLLYIPQVEFLGLMQKNVQVLTNYLNSISNRAQFLSNKLWFLSFKTIKEKIAHYLLTL
                     SKSEIRTTLVLPKSHQELAEFFGVTRPALARVFSEMQDEGIIHINRREITILNRNKLL
                     EMIK"
     gene            4903..5496
                     /locus_tag="PLW22_10735"
     CDS             4903..5496
                     /locus_tag="PLW22_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75218.1"
                     /translation="MPVKYQNGQVVCVQEHKPIAFNPWKHHCGFVCNQIQLIRGEGGG
                     KLNELLGYIGGTLLDFYIGELSVYTIVNEVNKYFMDMGVANVDDFKHWLGLPETTFKS
                     IYLSDGQCWVIRLGQNDEYYIHIHPGRHSKLTIRCRPTTLKTAIAFRYLFGFEDESLE
                     IDKLNYARKLAGLPPVESLNSASALQNFISNLRVNNR"
BASE COUNT         1573 a         1092 c         1183 g         1888 t
ORIGIN      
        1 cccttgcact tagggcagtg tcgtgggttg caaatacttt tacatggggt atgctctgaa
       61 tgaaatcatc gcattgcgca attgccatgg gatgtgagtg aatctccttt atatcctcaa
      121 ccctttgacc gcttatacct ataaggtgct gcgatatcct caggaaaact tccccctcaa
      181 tggttaaatc cgattctcga attagtgtgt agttctgcaa aatgcttcct actaatgagt
      241 tttcaattgc cattacacct ttttcggctc gtccttcggt aaccgagtta accaactctt
      301 tgaaggtaag gcattcaacc agttcgagag gtgtattgaa gtaaagttcc gctgcatcct
      361 gatggaaagc gcccttgaaa ccctgtattg ctacttttat tggttgcatt tgatttgcat
      421 taagttttat taaattaaaa agccctgttt ctggcagggc tcaattagta tcattaaata
      481 aatggagtag ttgagcccgc tggttagggg aagtagaaaa agtaatagta aaacataacc
      541 ttacatgagg ttacccttgt agtgggttgt atgtagatgc gtttactcat aaaaaaagcc
      601 ccgctattgc ggggctggtt tattgttaac tggttatacc tttttataat atacaacaat
      661 tacccagccc acaattacta aagtagaagt agtagctgta aaagtagttg ttgtttattg
      721 ctctcattct tttcttcctt gttttgcaat attaagtaaa gtaattaaca attcaaaaga
      781 atgaataaga ttatttttgt aagaatttca tttttaatag ttggctatcg gtatgattaa
      841 aatattgctt ctggataatt acgattcgtt tacttacaat ctttaccatt atatcgattt
      901 aaccggtggc tttatcgttg atgttattcg taacgatgct ctaaaacttg ccagcattaa
      961 tagttacgac ggaattgtgc tttcgcccgg acccggatta cccaaagaat cgggtttact
     1021 aatgaatgta attgataact accactcaaa aaaaaagatt ttcggcgttt gtttagggca
     1081 tcaggctctt gctgaatatt ttggggctaa actgctcaat ttgcctgaag ttttgcacgg
     1141 aatacaaacc ccgattaaaa tacttgagcc aaagcattat atttttcgtg atataccaag
     1201 tactattgaa gtgggtcggt accattcatg ggtagttaat cccattgatc tacctgattg
     1261 tttaacggtt actgccattg attacgacaa tcagattatg gcgttatgtc ataaaaaata
     1321 tgatatctgc tcagtccaat ttcacccaga atctattatg actccttacg gaatgaaaat
     1381 tatcttgaac tggcttgacg gtttttaggc gttctttatc atctcaattg ctgccaacgg
     1441 attatcggcc ttaaaaactg cgtttccggc tacaagcacg tttgcccctg cttggtaaag
     1501 ttcacgggcg ttgcttgggc ctacgccacc atcaacctct atcagggttg atagcccttt
     1561 ttggtcgata agctttctta atcgttcaat tctaccaatg ctgttgggta tgaatttttg
     1621 tccgccaaac ccggggttta ctgacatgat taaaaccaaa tcggcgtatt caagaatatc
     1681 ggttagcagt tcaacgggtg tgtgtggatt aactgaaaca cctgccttca ttccaagttc
     1741 cctgatacgt gaaagagtcc tattaaggtg tgtgcaagct tcgtagtgaa cggttagcca
     1801 gtcggcgcca gcctcgcgga aacgctcaag gtaacggtcg gggtcaacta tcattagatg
     1861 aacgtccaat ggtttagtag ccagagattt tattcgctca actactggga aaccaaacga
     1921 tatgttggga acaaatactc cgtccataat atccaggtga aaccagtcgg cttggctctt
     1981 gtttaccatt tcaatgtcgc atgaaaggtt ggcaaaatcg gccgaaagca tcgatggggc
     2041 tactaaacgt gtcatcatta tcttgttttt tgcaaaagta gctaaaaata aaaaaaaacg
     2101 acccgatggg ccgttaagta attggctatc ctaagtagct tttgagtatt ttgcttcgtg
     2161 tagtttgctt taagcgcttg agcgcttttt ctttaatttg ccgaactcgt tcgcgagtta
     2221 ggtcaaactc ttcgcctatc tcttcaaggg tgagaggatg cttcccattt aagccaaagt
     2281 acaacctgat gatatttgct tcgcgaggcg agagtgtcga taatgctcgt tcaatctctt
     2341 tgcgtagact ctcgttaagt agccccttat caggcatggg agagtcggga gaaaggagta
     2401 catcgtacat gcttccttct tcatcttgag taattggggc atccattgat aaatgacggt
     2461 tgttgccctt aagggcttcc ttaacatcat ctggtgcaag gtttagcatt tcggatagct
     2521 catcaatggt aggctccctt tcaaagcgtt gctctaactc ggctaatgca cggtttacct
     2581 tgtttataga accaatcttg ttaagtggca attttacaat tcgtgcctgt tcggcaaggg
     2641 cttgcagtat ggattgtcta atccaccaaa cggcatatga tataaactta aagcctcttg
     2701 tctcatcaaa gcgctgggca gccttaatta atccaacgtt accttcgttg atcaggtcgg
     2761 gtaggcttaa tccttgattc tggtactgct ttgcaaccga tactacaaat cttaaattgg
     2821 cttttacgag ttttgccagt gcttcctggt cgccttcttt aattttcctg gcaagcgtta
     2881 cctcctgttc ggctgttagc aaatcaacct ttccaatctc atgaaggtac ttgtcaagcg
     2941 agacagcctc tcggttagta acctgtttaa ttatcttcaa ttgcctcatg ccaatgattg
     3001 atttttaaac attcgaatat attaacaaaa attaaaaatt gttctttttg ataaattttt
     3061 tttatacttc gattaaacat ttgcgaaaac ttattgtcta aataaatttt tcttatggtg
     3121 tatggcatat ttatggtgac taatgataat tcaaatagta gtatcttcgt aatttaagtt
     3181 agtaactatg catttttctg ttattatacc ggtatataat cgccctggtg aggtagagga
     3241 attacttcaa agccttgcag gtcagggata taatagtttt gaggtaataa ttgttgagga
     3301 tgggtcaact gttccctgta gcgatatagt aaacaaattc gcctctgagt taaaaattaa
     3361 ttattacttc aaggaaaaca ctggcccagg gctaacacga aactatggtg ccgaaagggc
     3421 aaaaggggag tacttaatct tttttgactc cgattgcata atccctaaca attactttac
     3481 ccgtgttgag gaggaacttg cggcagaata tgttgatttt tttgggggcc ccgatgccac
     3541 tcacccctcc ttcaccaatg ttcaaaaggc tattggctac tccatgacct cattttttac
     3601 aactgggggt attcgagggg gaagggtaaa gctggagcgt tttactccac gtagctttaa
     3661 catgggcatt aatgccgaag ttttccattc ggttggtggt ttttcaaaca tgaggtttgg
     3721 tgaagatgtg gatttaagtt taagactttt cgagaagggt tttacgagca ggcttttccc
     3781 taaagccttt gtataccata aacgcagaac cgacttccga aagtttttca aacaggtttt
     3841 caactcgggt atggctcgca taaatctaag caaaaatcat aagggctcac ttaagatggt
     3901 tcacctttta ccctcgggat ttgtgttggt aatgagtgca gctattctgc tttcatgggc
     3961 tttccccatt atattaacgt tgcccttgct atacactttt gcaataatgt tcgactcctt
     4021 gttaaaagga aattcgctca cggttgcaat attaagcata gtggcagcat gggttcagct
     4081 atacggttat ggcttaggtt ttatttatgc tacctggtta tgggttgttc atggtaaaag
     4141 tcccaatggt gcttttacta aaaactttta ccggtagcta ttttatcatt tccagcaact
     4201 tgttgcggtt aagaatggtt atttcacgac ggttgatatg aataattccc tcatcctgca
     4261 tctcggagaa aacccttgcc aaagcaggac gcgttacccc aaaaaactct gccaattcct
     4321 gatgcgattt tggaagcacc agtgtagttc taatttcgct ttttgatagt gttaataggt
     4381 agtgcgctat tttttccttt atggttttaa atgaaagaaa ccaaagtttg tttgatagaa
     4441 actgagctcg gtttgagatg gagttaagat aattggttaa cacctgaacg tttttttgca
     4501 tcagtccaag aaactccact tgagggatat acagcagttt gcagttttcc aaggcaataa
     4561 catcaacagg gtacctgttg ttttcgccaa aaagaaaagc gtgggcaatt ggcattggag
     4621 catgcatctc ctcaattttt agaatttttc cagaaaagtc aaccatttcg ccctttacgc
     4681 tcccctcaac taaaatgcac aaatttcgga cctcttcggt tcgctgggct atggtttgcc
     4741 ctttactgaa tgagcgaatg gtgtggggtg tttcatttag tagatgggct acttcattct
     4801 cagttaatcc ccgaaatatg ggtgataatg ctataacctt gttcataatt ttcttaattt
     4861 gccacaaatt aaacaaatca acaggtagtt tgcttaataa atatgcctgt taaatatcaa
     4921 aacggacagg tggtatgcgt acaggagcat aaacctattg ctttcaaccc ttggaagcac
     4981 cattgtggtt ttgtatgcaa tcaaattcag ctaatcagag gggaaggagg tggcaagcta
     5041 aatgagcttt tagggtatat tggagggact ctactcgatt tttacattgg tgaactttct
     5101 gtttacacaa tagtaaatga ggttaacaaa tattttatgg atatgggtgt agccaatgtt
     5161 gatgatttta aacactggct tggcttacct gaaactacgt tcaaaagcat ttatctaagc
     5221 gatggtcagt gctgggttat tcgattagga caaaacgatg aatattacat ccacatacac
     5281 ccggggcgac actctaagct gactatccgc tgtaggccaa caactctaaa aactgccatt
     5341 gctttcaggt acctttttgg atttgaggat gaaagtttgg aaattgacaa actcaattat
     5401 gcccgtaaac ttgcaggttt acccccggtt gaatcgttaa attcagcttc agccttgcaa
     5461 aattttatat ccaatttaag ggttaataat cgttaaaaat ccacatgtgt tttataacat
     5521 atactgctga atatcagtat atacaagtcg ttcaaaaaag gtgttttata tcatcatttt
     5581 tggcctgaat tttgtgaaac tatggttgta tttttgcgtt ataaaagtag tttttcaagt
     5641 ataggtttta gggttaatag ttggttaggt ttagttgatt taggttagtt agggtgtaag
     5701 gacggagccg tattttcggt tccgtcttta ttttta
//
LOCUS       DAOOWJ010000139         5733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_14185_length_5733_cov_8.879183, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000139 DAOOWJ010000000
VERSION     DAOOWJ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14185_length_5733_cov_8.879183"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PLW22_10740"
     CDS             <1..1281
                     /locus_tag="PLW22_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75219.1"
                     /translation="LKSKAWSEHPLLAYPVLSSIPDLTNRNPVEAQSLKAFLIKEEKG
                     LAEFLMRYEEWAKTNLPNYAPLPQGLEFKHTGNQSDIVYRFLTALRLNPNIKLRLYLH
                     LLPNQDINGKTVIPPSELTTLTDVSSLSHTNYVVLNEGELVAPLQVLCTANDEPDYGF
                     DIGLFEDNGTNYGKTYGFGVQPFGDPKLEYGSQAPFHMGFYHEAKLVYAFGPFLKKTY
                     PEYRISLFKALAEYAFATGNDYWGWRFMGWGMHYLGDLSMPYHTAPLPGMSAARMIWI
                     NLKAIIGFPKSKNDAVQLVSNKHSVLEQYQWIAMREAYSNYDTNPLIEALRAEPPITP
                     YSNDFARNVVSQISVDRSRKVDKALKEFCPPLLVKDPSFEASKSKELDNLMDQINNYS
                     GANGIEQLNALLTEMLRTYSMSLRSYYNAIVSKA"
     gene            1321..2031
                     /gene="pyrH"
                     /locus_tag="PLW22_10745"
     CDS             1321..2031
                     /gene="pyrH"
                     /locus_tag="PLW22_10745"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOU75220.1"
                     /translation="MIKYKRILLKLSGEALMGDDKYGINTQVLTSYAKQIKEVADLGV
                     EIGIVIGGGNIFRGLSGVSKGFDRVKGDQMGMLATVINSLALQSAIEGAGGKAKVFTA
                     TKMEPVGELYTKAKVIEGFKQGFVAIIAGGTGNPFFTTDTASALRGVEIEAEVLLKGT
                     RVDGVYTADPEKDKTAVKFDELTFTDAYEKKLKVMDLTAFTMCSENNLPILVFDMNTP
                     GNLMKVVTGERIGTLVKN"
     gene            2069..2635
                     /gene="frr"
                     /locus_tag="PLW22_10750"
     CDS             2069..2635
                     /gene="frr"
                     /locus_tag="PLW22_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOU75221.1"
                     /translation="MNLDDAKKVIDIAESKMQKAIEHLDNELRVIRAGRANPAMLSGV
                     MVDYYGTMTPLTQVASVGSPDARTIVVQPWEKKMIDPIEKAIMAANLGFNPQNNGESI
                     RIMVPPLTEERRKDLVKQVKHEGENARVSIRNARRDALEEIKKMQKNGLPEDAAKDAE
                     TKIQKITDNYNKKVDDVLHKKEQEILTV"
     gene            3926..5014
                     /locus_tag="PLW22_10755"
     CDS             3926..5014
                     /locus_tag="PLW22_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOU75222.1"
                     /translation="MKNIAIKVDRHHNEEVICFIFEKDIQLIGILKTKLNAHWSATKK
                     CWYLPISQFSLYQAFEAFKSIAWLDIKNLPHTSNKDETQIDIRQEKERFEKWLKFKRY
                     SPNTIKTYTDALVVFLSYFNHKPVSELTNDDVVLFVNQYILKHKYSFSYQNQIVNALK
                     LYFREIRKSKIEVEGLQRPRREHKLPNVLSKEEIKAILEAPTNIKHRTMLSLIYACGL
                     RRSELLNLKIDDVDSKRHMLIIRNSKGYKDRQVPISDKTIEMLRTYYKAYKPQKWLFE
                     GDQINEQYSATSLQKVFKTALHKAGIKKNATLHWIRHSYATHLLEAGTDLRYIQELLG
                     HKSSKTTEIYTHVTEKSLQKIKSPFDDL"
     gene            5325..5630
                     /locus_tag="PLW22_10760"
     CDS             5325..5630
                     /locus_tag="PLW22_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75223.1"
                     /translation="MTNESQNSSNQAGQTVIINQTEKKSNGVGTAGFVLALIAVFLGW
                     VPVLGWIIWLLGLILSFVGVFKQPKGLAIAGLVISLIGIILLLTVFGAILGGAAAFL"
BASE COUNT         1891 a         1179 c         1235 g         1428 t
ORIGIN      
        1 cttaaatcaa aagcatggtc ggaacacccg ctactggcat acccggttct gagcagcatt
       61 cccgacctca caaaccgcaa ccctgttgag gcgcaaagcc ttaaagcttt tcttataaaa
      121 gaagaaaagg ggttggctga atttctgatg cgatacgagg agtgggctaa aaccaactta
      181 cccaactatg ctccattacc acagggcctt gagtttaagc acactggtaa ccaaagcgat
      241 attgtttacc gttttctcac agccctcagg ttaaacccta atatcaagct gagattgtac
      301 ttacatttac ttccaaacca ggatattaat gggaaaaccg ttattccgcc ctcggaatta
      361 accactctga ccgatgttag ttcgctaagc cacaccaact acgtggtgct taatgaaggc
      421 gaattggttg ctccgctcca agtgctgtgc accgccaacg acgagcccga ctacggattt
      481 gacataggtt tgtttgaaga taatggcacc aactacggaa aaacctatgg attcggcgtt
      541 caaccctttg gcgatcctaa acttgagtat ggcagccagg ctccttttca catggggttt
      601 taccacgagg caaagctggt ttacgccttt ggcccatttc tcaaaaaaac ctaccccgaa
      661 taccgcataa gccttttcaa ggcactggct gagtacgcat ttgcaaccgg caacgactac
      721 tggggttggc gattcatggg ctggggaatg cactaccttg gcgacctatc gatgccgtac
      781 cacacagccc ccctacccgg aatgagcgcc gctcgaatga tttggattaa cctcaaggct
      841 attattggct tcccaaaaag caaaaacgat gccgttcagc tggtatcaaa taaacactcc
      901 gtacttgagc agtaccaatg gatagccatg cgtgaggcat atagcaacta tgataccaac
      961 ccactaattg aagcgcttag agctgaaccc cccattacac cctattccaa cgactttgcc
     1021 cgtaacgtgg tgagccaaat atcggtcgac cgaagccgta aggttgacaa agccctaaaa
     1081 gaattctgcc ccccacttct ggtaaaagac ccatcgtttg aggccagtaa atcaaaagag
     1141 cttgacaacc ttatggatca aatcaacaat tactcaggag caaatgggat tgaacagctt
     1201 aacgcattgc ttactgagat gctccgtacc tacagcatgt cgttacgaag ctactacaac
     1261 gcaattgtta gcaaagcata accgataatt tgctattttt acgccctaaa aatgtaaaca
     1321 atgataaagt ataaacggat actgcttaag cttagtggcg aagcgcttat gggcgacgat
     1381 aagtacggta ttaacaccca ggtgcttacc agctatgcca aacagataaa ggaggttgct
     1441 gaccttggcg ttgaaattgg gattgttatt ggtgggggaa atatttttcg gggtttgagt
     1501 ggcgtttcca aaggattcga ccgggtaaag ggcgaccaga tgggaatgct ggccacggta
     1561 attaatagtt tggcgctaca atcggccatt gagggagcag gcggaaaggc taaggttttt
     1621 accgccacca agatggagcc cgttggcgag ctctacacca aagcaaaggt tattgaaggg
     1681 ttcaagcagg gcttcgtggc gattattgca ggcggaaccg ggaatccttt cttcacaacc
     1741 gatacagcct cagcattacg cggtgttgag attgaagcag aggttctcct gaaaggcacc
     1801 cgtgttgatg gtgtttacac tgccgatccc gaaaaggaca aaacagccgt aaagttcgat
     1861 gagctaacct ttaccgatgc ttacgagaaa aagttaaagg taatggatct taccgccttt
     1921 accatgtgta gcgagaataa ccttcctatc cttgtattcg atatgaacac cccgggtaac
     1981 ctaatgaaag ttgtcactgg cgaaagaatt ggtacattgg taaaaaatta atacttttag
     2041 tgtccgaaac aataaaaatc ataaagccat gaaccttgac gatgcaaaaa aagttataga
     2101 tattgccgag agcaagatgc aaaaggccat tgagcatttg gataatgagc ttagggttat
     2161 cagagccgga agggcaaacc cggccatgct ttccggtgtt atggttgact actacggaac
     2221 aatgaccccg ctcacccagg tggcaagcgt gggcagcccc gatgcccgca ccattgtggt
     2281 gcaaccctgg gaaaaaaaga tgatcgaccc cattgagaaa gccatcatgg ccgccaacct
     2341 tggctttaac ccacaaaaca acggcgaatc gattcgcatt atggtacctc ccctaaccga
     2401 ggagcgccgc aaggatctgg ttaagcaggt aaagcacgag ggtgagaatg cccgcgtgag
     2461 cattcgcaac gcccgccgcg atgcccttga ggaaatcaag aagatgcaga aaaatggcct
     2521 tcccgaggat gctgctaagg atgctgaaac caaaatccaa aagattaccg acaactacaa
     2581 taagaaagta gatgatgtgc tccacaaaaa ggagcaggaa atacttaccg tttaggattt
     2641 gaacgttccg taatgcaagg gtgttactta accggtaaca ccttttttta tccatcacat
     2701 ccttagcctc gatatacagg gaaaatcttt gtcgaggaat cccatattta gtgtaccgtg
     2761 ttcagtgtgc agtgtacagt ggggggtgat gtttaaggat aaaggataaa aatttaaaat
     2821 ttcagatctc agatttcaga ttcatgattg aaggtggttt cttaagccat caaccaacaa
     2881 caatcaacta aattgttatc acagagttac acagtgtaac atgttgttac agaatggcat
     2941 tcacgaacaa cgatacacga acaaccagca actactcctg tcatcttgag cgagtcgagg
     3001 aatcgcatta atgaagagtt gtaggttcaa tgagatgctt cggctggact cagcatgaca
     3061 aggtgagtga ggaagctaag ggtgttgtca tcccgagcgt agccgaggga tctcgtaaaa
     3121 gaataaaagg ggaaagaaat gcttcggctg gactcagcat gacaaggtga gtgaggaagc
     3181 taagggtgtt gtaatcccga gcgttgtcaa gggatctcgt aaaagaataa aaggggaaag
     3241 aaatgcttcg attgggctca gcatgacaat agtcttatga accgatcaac atcaaatttc
     3301 agagagtgta tgacttagct tacacgaaca acgagcaacg agcaatgaac aacctttcca
     3361 gccccgtagg ggcgacatat ttgtaaccca atggcttctc cctcgtgtgt tgcgatgcac
     3421 gtaatgtata tgccaaagag caaggtggaa gcgttgcatc gcaactgaag agcatggaaa
     3481 agagcggttt ccaaaaaaca acaagaatta aagggtttaa tgtaaagaga tgcttcggct
     3541 tgctcagcat gacaagtgag cagaaaggaa gatagggttt ttgtcatccc gagcaaagtc
     3601 gagggatctc ttcaaaggtt tgaagagaaa agagcaatga gatgcttcgg cgaagtctat
     3661 ccctttaaat cgggactcag ctcggcagcg tgatgtttcg cttgcgcaaa acaagacaaa
     3721 taaccttaac taaacaacat tgatatctaa ctactaaccc tatcatcaca gacataaaca
     3781 cagatacatc aattgatatg atcaattgat gtgtctcgaa taaataaatt ctttaacttt
     3841 actaaatgaa ttatgtgaca aattgataaa aacaatcatg tgttcagtaa agtataagta
     3901 aggcactaat atttacccct tgaacatgaa aaacatagct ataaaagttg atagacacca
     3961 caacgaagaa gttatatgct ttatatttga aaaggatata cagttaattg ggatacttaa
     4021 aactaagctc aatgcccatt ggagtgccac aaaaaaatgt tggtatctgc ccatttcgca
     4081 gttcagttta taccaggcat ttgaagcatt taaaagcata gcctggttgg atataaagaa
     4141 tctacctcat acatcaaaca aagatgaaac ccaaatagac attcggcagg aaaaagaaag
     4201 gtttgaaaaa tggctcaaat ttaaacgcta tagccccaat acaatcaaaa cctatacaga
     4261 tgctttggtt gtttttctca gttattttaa ccataaaccg gtttctgaat tgacgaacga
     4321 cgatgtggta ttatttgtaa accaatatat actaaagcac aagtatagct tttcgtatca
     4381 aaaccaaata gttaatgcac taaaacttta cttccgggaa atacgcaaaa gcaaaataga
     4441 ggtagagggg ttgcaacgcc ctcgtcggga gcacaaatta cccaatgtgc taagcaaaga
     4501 agagattaaa gctatactcg aagctccaac aaatataaaa catcgcacca tgctaagcct
     4561 tatatatgcg tgtggacttc gccgcagcga actattgaac cttaaaattg atgatgtgga
     4621 ctcaaaacga cacatgctga taattagaaa ttcgaaagga tacaaagacc gccaggttcc
     4681 tatttccgat aaaaccatag aaatgctgcg tacatattac aaagcgtaca aaccccaaaa
     4741 atggttgttt gaaggtgatc aaattaatga acaatattct gcaacaagtt tacaaaaggt
     4801 ttttaaaaca gcgttacaca aagccggaat taaaaaaaat gcaacattac attggataag
     4861 gcactcttat gccactcatt tattggaggc tggtactgat ttaaggtata ttcaggaatt
     4921 acttgggcac aagagttcaa agactaccga aatatacacc catgtaactg aaaaaagttt
     4981 acaaaaaata aaatcaccat ttgatgattt ataaaaattt tttatattag cgaaaataat
     5041 atacacctgt tttttgccaa aaagtgcaca atacattcca atttggatgt atttgtacac
     5101 ctaaaattaa aaaaaggtgt atatataaac gagttatggg caagttaacg acacacacaa
     5161 cagcatgaca cagacaagag aacctccgat ttttgataca tcttgacaga aattataact
     5221 tcaagcaaaa taatctacca aaaaaagtgg agactagcga ccacttaaaa aaacggggcg
     5281 acaccgaaaa gccatatgag tagggaaatt taaatcttat tattatgaca aacgaaagtc
     5341 aaaattcctc aaatcaagca ggacaaacag taattattaa tcagacggaa aagaaatcaa
     5401 atggcgttgg aactgccggt ttcgtacttg ctttaatagc agtattttta ggttgggttc
     5461 cagtattagg atggataatt tggttattag gactaatatt gtcttttgta ggtgtattca
     5521 agcagcctaa agggttagct attgcaggtt tagtgatttc tttaataggg ataattttat
     5581 tacttacggt gtttggagca attttaggtg gtgctgcagc cttcctatag agttcctacc
     5641 ttttctgaaa tttaatatca ggaaagacaa ggaaaaccag cccataacat gcggtatgtt
     5701 ttattgccga gataatgctg aaatcaacgg ctg
//
LOCUS       DAOOWJ010000140         5676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_14383_length_5676_cov_6.721402, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000140 DAOOWJ010000000
VERSION     DAOOWJ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14383_length_5676_cov_6.721402"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLW22_10765"
     CDS             <1..685
                     /locus_tag="PLW22_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU75224.1"
                     /translation="NGLTLSKMALCQQVGLLLNTDIVIADSLPALMEPIAQLNYTDSA
                     LARRPEIEALNQTINIAKSGVNLMKSRYMPNIGLTANYMFFNPNPYNGLTENFGGDWS
                     LGVAINVPIFHWNDRGHTLKAARSEQRVAELKMDEAKELIGLQVQQAIFQLNESIKKV
                     EMAQVNLKQAEENLKVARDAFESGRQKTSDVLEAQAMWQNAFSDLIDARMEYRLNVVN
                     LKRVTGSLK"
     gene            750..1793
                     /locus_tag="PLW22_10770"
     CDS             750..1793
                     /locus_tag="PLW22_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU75225.1"
                     /translation="MKRLILLFTAAAALVFVSCSGNKQEQAQSEVNVPLVSTIRVEER
                     TFNPVLTFTGTVFAYKEANLGATLPGKVEKIYFEEGQMVKKGDLIVELSDEMLTQAMI
                     EHETIKKDFERVSRLREKGSISEMEYDHVKAKYDASTAKVEMVKKNTQVYAPFSGIIA
                     ERMMEEGEVYFLNPGLEPGYSMRSGIVRLMQIDPATVKFDVNEKDLIHIRKGLPVVVK
                     LDGMPGKTFQGKVDRIKPMLSTTTRSTSVEVTIPNSNMEVKPGMFAFVDVMLPEVKSA
                     VVPLRSIYRMPGTSDDFIFTVVNDTVHRVKVERLQTIGEHVAVRGIDAGTEIILEGKN
                     RVNDGLKVKVSNR"
     gene            1817..4945
                     /locus_tag="PLW22_10775"
     CDS             1817..4945
                     /locus_tag="PLW22_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU75226.1"
                     /translation="MKLPQVAVKRPVFTAMLFVAILLFGVVSLTRLPLDIMPEMELPT
                     LTVITVYPGASADEVEEQVSKKLEKILSGTEHLKEITSQSKENVSFVQLQFEWGTDIT
                     SAANNARDLIELVKNKLPRDAHNPIIFKVNSSLMPVLVYGVTANESYNGLYKIVDDEV
                     ASKLRKVPGVGSVIVIGAPEREIKVDIDPVKLHAYNLSLNQVATVLKAQNISIPGGNI
                     KVGNDDYSVRIPGDIASVDELRDMALISFNGKVIRLSDVAEVRDEYKEKDDVGRTTNG
                     KGVTLMVQKQSGENTLEVVKKIRKKVAEIKPALPSDVSITEVMKSDELITESINNLSE
                     SLWYALFFVVLVVMAFLRNWKQSFIIFITIPFSLIVAFIVMFASGWTINIFSLMSLVV
                     ASGMVVDNAIVVLENITQHIEKGAKPKQAAIFGSSEMGMAISASTLTTIVVFLPMIFM
                     GGIVGIMFKQLAILTSVTLIASLFTALTLTPMASSQMLEGMKKNEKRRHGKLFEWSER
                     MFQKLENGYKKALAWVVHHKTFTVIVTVALLAFSLWVGKGLGTDYIPEFDAGDVVAVI
                     ETEVGTSTTKTDSVAQLVMQIFQDEIPEMVPGSLASVSGQTEEGLLSSVGFSEGKNIA
                     TVMCHLSKPNERKHSAKQIADRVRPRIAAIPEVENFRLLGGSIISAAVTGNKKPIEIE
                     VSGNSFGSINSTAEAITARMRQSKYFSDVINNVDKGKLEVQIRVDKRKASAMGLNSAM
                     IGLQVRQSIYGTEAGEFKEGGDEYDITLRYSPEYRNDISQIRNIQLKNLLNQTIPLSA
                     VAEVEMGTSPLQINRKGQQRVVKVMAELNDVSLGDAHKEAKRILAGLDIPSDVTVEIA
                     GQTTDQGESFGDLYLILALGVLLVYMVMASQFESFRDPFIIMFAVPVTFIGVIWAFKL
                     IGLTLSITTFVGLIMLMGIVVNNGIVLVDYTNLLRKRGYKLYDAIAEAGRSRLRPVLM
                     TTFTTLLGMVPMATSSGMGREAYSPLGITMISGLLVSTLITLLLVPTIYAIFHDNERK
                     AEVDTNKSVLFN"
     gene            4959..5264
                     /locus_tag="PLW22_10780"
     CDS             4959..5264
                     /locus_tag="PLW22_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75227.1"
                     /translation="MKMIYCTCNVAVLEPLLKMLDDNNVRDFQVVEQVTAKNKKGDHR
                     FNNPVWPGYNSTVFIQITEDEKAKMVMQKIREFNRNAFNDNELVTACMWSIEDYFFD"
BASE COUNT         1591 a         1221 c         1380 g         1484 t
ORIGIN      
        1 gaacgggctt accctatcca aaatggctct ttgccaacag gtaggattac tactaaacac
       61 cgatattgtt atagccgatt cattacctgc tctaatggaa cccatagccc aattgaacta
      121 caccgactcg gctttggccc gccgtcctga gattgaggca ctaaaccaaa ccatcaatat
      181 tgccaaatca ggcgttaacc taatgaaatc gcgctacatg cctaatattg gattaacggc
      241 caactacatg ttctttaacc ccaaccccta caacgggcta accgagaact ttggtggcga
      301 ctggagttta ggtgtggcta ttaacgttcc cattttccac tggaacgatc gcggccatac
      361 cctgaaagca gcccgaagcg agcagcgtgt ggccgaactt aaaatggacg aagccaagga
      421 acttattggc ttacaggtgc agcaggccat ttttcagctc aacgagtcaa tcaagaaggt
      481 tgaaatggca caggtaaact taaaacaggc cgaggaaaac ctaaaggttg cccgcgatgc
      541 ctttgagagt ggccgccaaa aaacttccga cgtgcttgag gctcaagcta tgtggcagaa
      601 tgctttcagc gacctgattg atgcccgcat ggaatacagg ctgaatgtgg ttaatcttaa
      661 acgcgttaca ggaagtttga aatagactgg ttaaacgcac ttctgaaatt ttgaatttgg
      721 aattttgaat caattaaaaa tattaaacca tgaaacgatt aattttacta tttacagcag
      781 ctgctgcgtt ggtttttgta tcgtgcagtg gcaataagca ggaacaagcc caaagcgaag
      841 tgaatgttcc tcttgtatcc accatcaggg ttgaggagcg cactttcaat ccggttctaa
      901 cctttacagg tacagtattt gcctacaagg aggcaaactt aggcgctact ctacccggaa
      961 aggttgaaaa aatctacttt gaggagggcc agatggttaa aaagggcgat ctcattgtgg
     1021 aactttccga tgagatgctt acccaggcaa tgattgagca cgaaaccatc aagaaggatt
     1081 ttgagcgtgt atcgcgctta cgagagaagg gaagcataag cgagatggag tacgaccatg
     1141 ttaaggctaa atacgatgct tcaaccgcaa aggtcgaaat ggttaaaaag aacacacagg
     1201 tttacgctcc cttcagcggc ataattgccg agcgaatgat ggaggaagga gaagtgtact
     1261 tccttaaccc cggtctggaa cccggctatt ccatgcgttc gggcattgta aggctgatgc
     1321 aaattgaccc tgccactgta aagttcgatg tgaacgaaaa ggatttaatc catatccgta
     1381 aaggcctacc tgtggtggtt aagctcgatg gtatgcccgg aaaaaccttt cagggaaagg
     1441 ttgatagaat taagcccatg ctctcaacca caacccgttc cacatcggtt gaggttacca
     1501 ttcccaactc aaacatggag gttaaaccgg ggatgtttgc ctttgttgat gtaatgctac
     1561 ccgaggtgaa atcggcagtg gtgcccctgc gtagcattta ccgtatgcct ggcacatcgg
     1621 acgattttat atttactgtg gttaacgata ccgttcaccg tgttaaggtt gagcgcctgc
     1681 aaaccatagg tgagcatgtt gccgttaggg gtatcgatgc aggtaccgaa attatcctcg
     1741 aaggcaaaaa ccgcgtgaac gacggtttaa aggttaaggt gagcaacagg taaaaatcct
     1801 aaaaaaacgg aagaacatga aactaccaca agttgccgta aaaaggcctg tattcacagc
     1861 catgttgttt gtggcaatcc tgctctttgg cgtggtatcg cttaccaggc taccgctcga
     1921 cattatgccc gaaatggagt tacccactct tacggtaatt actgtatatc cgggcgccag
     1981 cgccgatgag gtggaagagc aggtatccaa aaagttggaa aaaatcctat cgggaacgga
     2041 acacctaaag gagataacat cgcaaagcaa ggagaacgta tcgtttgttc agcttcagtt
     2101 tgagtgggga accgatatta cttcagcagc caataatgct cgcgacctta ttgagctggt
     2161 aaagaataaa ctcccccgcg atgctcataa cccaattatt ttcaaggtaa acagttcact
     2221 gatgcctgtt ttggtgtatg gcgttaccgc caacgagagc tataacgggc tttacaagat
     2281 tgttgacgat gaggtggcca gcaagctacg caaggtgccg ggagtgggct cagtaattgt
     2341 aataggcgca cccgaacggg agattaaggt tgatatcgac cccgttaagc tacacgccta
     2401 caacctgtcg ctcaaccagg tggcaaccgt gctaaaggca cagaatattt ctatccccgg
     2461 tggaaatata aaggtgggca acgatgacta ctcggttcgc attcccggcg atattgccag
     2521 tgttgatgag cttcgcgata tggctctaat cagcttcaac ggtaaggtta ttcgccttag
     2581 cgatgtggcc gaggtgcgcg atgagtataa ggagaaggac gatgtgggaa gaaccacaaa
     2641 cgggaaaggg gtcaccctga tggtgcaaaa gcaatcgggt gagaacaccc ttgaggtggt
     2701 taagaaaata cgcaaaaagg ttgctgaaat taaacccgcc ctaccatcgg atgttagcat
     2761 taccgaggtg atgaagagcg acgagcttat cactgaatcc atcaacaacc tttccgaatc
     2821 gctctggtat gctctgttct ttgtggtgct tgtggttatg gcatttctgc gtaactggaa
     2881 gcagagcttt atcattttca taacaatacc cttctcgctg attgtggcat tcattgtgat
     2941 gtttgcttca gggtggacaa tcaacatctt cagcttgatg tcgttggtgg tggcatcggg
     3001 tatggtagtg gataatgcca ttgtggtgct tgagaatata acccagcata ttgagaaagg
     3061 ggctaagccc aagcaggcag caattttcgg tagtagtgag atgggtatgg caatttcagc
     3121 atcaaccctt accaccattg tggtattcct gcccatgata ttcatgggtg gcattgtggg
     3181 tatcatgttc aagcagctgg ccatactaac ttcagtaacc ttaatagctt cgttattcac
     3241 agcgcttacc ctaaccccaa tggcatcgtc gcaaatgctt gagggcatga aaaagaacga
     3301 aaaacgccgc cacggaaagc tattcgagtg gagtgaacgt atgtttcaga aacttgaaaa
     3361 tggctataag aaagcattgg catgggttgt tcaccacaaa acatttactg ttatagtaac
     3421 ggttgccctg ctggcattca gcctttgggt tggtaagggc ttgggaaccg actacatccc
     3481 tgagttcgat gctggcgatg tggttgcagt aattgaaacc gaggtgggaa catcaaccac
     3541 caaaaccgat agcgtggctc aattggttat gcaaatcttc caggatgaga ttcctgagat
     3601 ggttcccggc tcattggcat cggtttccgg acaaaccgag gaggggcttt tatcgtcggt
     3661 agggtttagt gagggtaaaa atattgctac ggtgatgtgt cacctttcaa aacctaacga
     3721 gcgaaagcat tcagcaaaac agattgccga cagagttcgc ccccgcattg cagccatacc
     3781 cgaagtggag aacttccgct tgcttggggg tagtatcatc tcagctgctg ttacaggaaa
     3841 taaaaaacct attgagattg aggtttcagg caacagtttt ggcagtataa acagcacagc
     3901 cgaagccatt actgcccgca tgcgccaatc caaatacttt agcgatgtta tcaataacgt
     3961 tgataaggga aaactcgagg ttcaaatcag ggttgataag cgcaaggctt cggccatggg
     4021 cctgaactca gccatgattg gcctacaggt tcgccagagt atatatggta ctgaggccgg
     4081 cgagtttaag gagggaggcg atgagtacga tataaccctg cgctactcgc ccgagtaccg
     4141 taacgatatc agccaaatcc gcaacataca gcttaaaaac ctgctgaacc aaaccatacc
     4201 gctatcggct gtggccgagg tggagatggg aaccagccca ctccagatta accgtaaagg
     4261 gcaacaacgt gtggttaagg ttatggctga gctaaacgat gtttcgctag gcgatgcaca
     4321 taaggaggct aaaaggattc tggccggctt ggatattcca agcgatgtta ccgtagaaat
     4381 tgctggccaa accactgacc aaggggagtc atttggcgac ctttacctca ttcttgctct
     4441 tggcgtgttg cttgtttaca tggtaatggc atcgcagttc gagagcttcc gtgacccatt
     4501 catcattatg tttgctgttc cggttacctt tattggggtc atttgggctt tcaagctaat
     4561 cggtttaacc ctcagtatca caacttttgt gggtttaatt atgcttatgg gtattgtggt
     4621 gaataacggt atagtgctgg ttgactacac caacctgcta cgtaagcgtg gttacaagct
     4681 ttacgatgcc attgccgaag caggccgaag ccgtttgcgt ccggtgctta tgactacatt
     4741 tacaacgctt ttgggcatgg tgcctatggc taccagcagc ggtatgggac gcgaggcata
     4801 ctcaccactt gggatcacca tgattagcgg gctactggtt tcaaccctta tcaccctgct
     4861 gcttgtgcct accatttatg ccattttcca cgataatgag cgtaaggccg aagtggatac
     4921 caataaatcg gttttgttta actaaattga tgttagctat gaagatgata tattgcacct
     4981 gcaacgttgc tgttcttgag ccattactca aaatgcttga cgataacaac gttcgcgact
     5041 ttcaggttgt ggagcaggtt acggccaaga ataagaaagg cgaccaccgt tttaacaatc
     5101 ccgtttggcc cggatataac tctacggtat ttatccaaat caccgaggat gagaaggcta
     5161 aaatggttat gcagaaaatc cgcgaattca atcgtaatgc atttaatgat aatgaactgg
     5221 ttactgcttg tatgtggtcc attgaggatt actttttcga ttaatttttt acgttgttaa
     5281 atcacgttaa agcccccagt taattggggg ctttaataaa atttatgatt atccgcttta
     5341 gtaacaatat tatttttacg ttattgcgat ccaggcgttg tttgatggga gaagcaatca
     5401 ttggcattga tttacaataa attgcttctt ctggaaaagt actccctgcc tgccagcaaa
     5461 ggcagggcaa ggcaagggca ggctcgcaat gtcaaattca ttattatttt gttgtatcta
     5521 cttaaaaagg gataaattac gatatacttt tcagaaaata gttgttaaaa atatgtataa
     5581 ttcactggta ttcatcgtat ttatgtcata agatcaatat ctatagttct tatgaatatc
     5641 attaaattca gtgcacaatt ccccgacgaa aagtct
//
LOCUS       DAOOWJ010000141         5672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_14397_length_5672_cov_7.065693, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000141 DAOOWJ010000000
VERSION     DAOOWJ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14397_length_5672_cov_7.065693"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PLW22_10785"
     CDS             <1..1638
                     /locus_tag="PLW22_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU75228.1"
                     /translation="FLLFNRDSKGNYFSYEAILDSLSRNVKAPIYGTWSFYLNHGIVG
                     GAIISGYHHGKRAGELASLLLKGVSPKTIAPTDGPSDFIVDHLQLVKYSISQKLLPQG
                     TLILNKNKRFIHLSRGTTLVVAAIVLLLIVLLIFMGVYSRMRHKALRLQKYYNTLLEQ
                     KNQEVENSLKFAEEANELKSAFLANMSHEIRTPMNAIVGFSKLIKESSLLSPEEVNGY
                     LDIIAENAQRLMRLINDIIDISKIDSKQLSIYKRPVQLGPLFANCYRAAEVELDRVNK
                     NNIAIEMSFRPTDAALEVVTDPDRLQQVVMNLLNNAIKFTSSGRIVLGYSVINDLLRV
                     SVEDTGIGIDSQYHDAVFERFRQIDGTLNREYGGSGLGLSICKGIVEAMGGRIGVDSR
                     PEVGSTFWFEVPISLATPGQHVEVPVKASKSKLKNKVLLIVEDNEPSAILLREMLKGT
                     GAVLLQAATGKEVATILNDKVKIDIALVDVYLPDMSGYEVVTKIKQAQPNVRVIAQTA
                     NAMDTDRQKSLEAGCDEHITKPIDKDKLIELLSMLLS"
     gene            complement(1644..2552)
                     /locus_tag="PLW22_10790"
     CDS             complement(1644..2552)
                     /locus_tag="PLW22_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU75229.1"
                     /translation="MEQYYGELAALLTAFFWTVTALAFESASRKVGSLSVNLLRLGTA
                     FIFLSVYALISRGKAFPVDAGTHQWVWLSLSGLVGFVLGDLFLFQSYVVIGSRISMLI
                     MSLAPPMAAIIGWITMGETLTLKQAIAMAVVLAGIVLVVLERQSADENGNGKRVRFSY
                     PVGGILLAFGGALGQAGGLVLSKYGMAGYDVIPSVQIRVITGIVGFTLFFAFSNKWKN
                     LSEALRNGKAMKRLLIGAFFGPFLGVSFSLLAVKYTSTGVASALMSIVPVLIIAPAVV
                     VFKEKITAKELVGAVITVVGVALFFL"
     gene            complement(2674..2925)
                     /gene="rpsT"
                     /locus_tag="PLW22_10795"
     CDS             complement(2674..2925)
                     /gene="rpsT"
                     /locus_tag="PLW22_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOU75230.1"
                     /translation="MANHKSAEKRIRQTESRREHNRYYAKTTRNALKALRNTTDKAAA
                     MELLPKVSAMLDKLAKNNIIHKNKAANLKSSIQKHVNAL"
     gene            complement(2952..3023)
                     /locus_tag="PLW22_10800"
     tRNA            complement(2952..3023)
                     /locus_tag="PLW22_10800"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2988..2990),aa:Glu,seq:ttc)
     gene            complement(3114..3587)
                     /locus_tag="PLW22_10805"
     CDS             complement(3114..3587)
                     /locus_tag="PLW22_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75231.1"
                     /translation="MKRLLFILATVFVTYSAANAQDYKTGLGLRGGYPSGVTVKHFFA
                     QKSAVEGVLSFGWGGIGITGLYQLHNPIPDAPGLKWYYGFGAHFATANADKKNPFESE
                     YGSKVFLGADGVIGLEYTFKDAPICLGLDILPIFNIIESPGIWFNAGFSVRYTFK"
     gene            complement(3638..5002)
                     /locus_tag="PLW22_10810"
     CDS             complement(3638..5002)
                     /locus_tag="PLW22_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOU75232.1"
                     /translation="MRVHFIAIGGSAMHNLAIAMKLKGYQVTGSDDEIFEPSLSHLKN
                     HGLLPSHQGWNPSIITPEIEAIVLGMHARADNPELLRAKELAIKIYSYPEFLYEQSRQ
                     KTRIVVGGSHGKTTITSMIMHVLKHLNYKFDYIVGAQLKGFENMVSFSDDAPFAVFEG
                     DEYLTSPIDPRPKFHVYRPNIAIISGISWDHINVFPTFDIYREQFAKFIDCIEPNGKI
                     IYCADDPEVCRVVEESQRNDIKRIPYHTHPHGIANGIWSLDFNGTSIPLKVFGKHNMQ
                     NISAAKAACITLGVTEKQFYNAIETFEGASKRLQLLAQGTSTSVFLDFAHSPSKLKAT
                     IEAVRELYPARKLVVCFELHTFSSLTKDFLGQYNGTLTGADVAYVFFNPHALELKKLP
                     PIDPNDVIQGFGDPHPAVFTDANNLVDVLLQHNWGNSNLLIMTSGNLAGTDLQHLADE
                     VVKH"
     gene            complement(5040..>5672)
                     /locus_tag="PLW22_10815"
     CDS             complement(5040..>5672)
                     /locus_tag="PLW22_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOU75233.1"
                     /translation="FISDPHHRYLIWGIDVSAHTGKIDWEKVKDHKVDFAFIKASEGA
                     TLKDKSFRNNVTRAREAGIPVGAYHFFNFNRRGIAQAQNFLRAIDGVELDLPPVVDVE
                     EWGNVVRRPRKQIIADLVDFVELVEKTTGQKVLIYTNKDTFKWYISETFPEHDIWICS
                     FDHTPEIEKSWTFWQYHHEGRLKGIQGKVDFNVFYGNTIEWKNYLNNKKR"
BASE COUNT         1653 a         1209 c         1243 g         1567 t
ORIGIN      
        1 ttcttacttt tcaatagaga tagcaagggg aactattttt cctatgaggc aatactggat
       61 tcattaagca ggaacgttaa agctcccatt tacggtacat ggtcgtttta cctaaaccat
      121 ggaatagttg gaggagccat aatatcgggt taccatcacg gtaaacgagc cggagagcta
      181 gcaagtctgc tactgaaagg ggtttcgccc aaaactattg ctcctaccga tggcccatcc
      241 gattttattg ttgaccattt acaacttgta aaatattcca tttcacaaaa acttttaccc
      301 caaggaacgc tcatccttaa taaaaataaa aggtttattc atttaagcag aggcacaact
      361 ctggttgttg cagcgatagt tcttttgcta attgtgcttt taattttcat gggtgtgtat
      421 agccgcatga ggcacaaggc gctaaggttg caaaagtact acaacacttt gctggagcaa
      481 aagaaccagg aggttgaaaa cagcttaaag tttgcggagg aggcaaatga gctaaagagt
      541 gcttttctgg ccaatatgag ccatgaaatc cgtacaccca tgaatgccat tgttggtttt
      601 tcaaagctga taaaggaatc atcgctgcta agtccggaag aggtaaacgg ttatcttgat
      661 attattgcag agaatgctca aaggctaatg aggcttatta acgatatcat tgatatttca
      721 aagattgact caaagcagct ttccatctat aaacgccctg ttcagcttgg tcccctgttt
      781 gccaactgct atagggctgc cgaggtagag ctcgacaggg tgaacaaaaa taacattgcc
      841 attgaaatgt cgtttcgtcc caccgatgca gcccttgagg tagtaactga tcccgatagg
      901 cttcaacagg tggtaatgaa cctgctaaac aatgccatta agtttaccag tagtgggcgg
      961 atagttttag gttacagtgt tataaacgat ttactcagag tgagtgtgga ggacacaggt
     1021 attggtatcg actcgcagta tcacgatgca gtttttgagc gttttaggca gatagacgga
     1081 acactgaacc gggagtacgg cgggtcaggt ttaggcctgt caatatgcaa gggaatagtt
     1141 gaggctatgg gaggtaggat tggtgtggac tcaaggccag aggtaggatc aaccttctgg
     1201 ttcgaggtac ccataagctt agccacgccc gggcagcatg ttgaggtacc agttaaggct
     1261 tcaaagtcaa agttgaaaaa caaggttttg ctcattgttg aggacaacga gccctcggca
     1321 atccttttaa gggagatgct caagggtacg ggtgcagtgc tccttcaggc tgctacaggc
     1381 aaagaggttg caactatcct gaacgataag gttaaaattg atattgcgct ggttgatgtt
     1441 tacctacccg atatgtcggg ctacgaggtg gttacaaaaa ttaagcaggc tcagcctaac
     1501 gttagggtaa ttgcgcaaac agctaatgct atggacaccg atagacaaaa gtcgcttgag
     1561 gcaggttgcg atgagcacat cacaaagcca atagacaagg ataaacttat tgaactactt
     1621 tcaatgttac tttcctgatt ttcctaaagg aaaaacagtg caacgcctac cactgtaatt
     1681 actgctccta caagctcttt agcagtaatt ttttccttga aaacaactac agctggagca
     1741 ataattaaaa cgggtacaat cgacatcaag gctgacgcaa ctcctgttga ggtgtatttt
     1801 acggctaaaa gcgaaaagga aacacccaaa aacgggccaa agaatgcccc tattaatagc
     1861 cgcttcatag ccttaccgtt tcgtaatgcc tcggaaaggt ttttccactt atttgaaaag
     1921 gcaaaaaaga gagtaaagcc aacaattcct gtaataactc gtatttgaac ggaggggata
     1981 acatcgtagc cagccattcc gtacttactc aaaactaagc ctccggcttg tcccagcgca
     2041 ccaccaaatg ctaaaaggat acctccaaca ggatacgaaa accgaacccg tttcccattt
     2101 ccgttctcat cggccgattg gcgctccaat actaccaata ctatacccgc tagcacaaca
     2161 gccatggcaa tggcctgctt gagcgttagg gtttcgccca tggttatcca acctatgatt
     2221 gcagccatag gcggagctaa cgacattata agcatggaga tacgggagcc aataacaacg
     2281 tacgactgaa aaagaaatag atcgcctaaa acaaatccta ccaaacccga aagcgatagc
     2341 caaacccact gatgcgtgcc ggcatcaacc ggaaatgctt ttccgcgact tatcagggca
     2401 tacaccgaaa ggaatataaa ggcagtaccc agccttaaaa ggttaaccga aagcgaaccc
     2461 acctttcggc tggccgattc aaaggccagg gcggttacag tccaaaaaaa ggctgttagc
     2521 aaagcggcaa gctcgccgta gtattgttcc ataaattcac tttaataaat attacccagt
     2581 tggagtttaa tggagctgtt tggtttaata ctccataaaa taaattgggc tgtcccttaa
     2641 ggaacagccc attgtatctt gttcattaac ctgttacaga gcgttaacgt gtttctgaat
     2701 tgaactctta aggttagctg ctttattctt atgaatgata ttgttcttag ctaacttatc
     2761 gagcatagcc gatactttag gaagtaattc catggctgca gccttatcgg ttgtgttgcg
     2821 taaagccttt aaggcattac gggttgtttt tgcatagtaa cggttgtgtt ccctgcgaga
     2881 ctcagtttgt ctgattctct tttcagctga tttatggttt gccatcgcta aaaaagtatt
     2941 ttcatgttta gtagcccgta ggggaatcga acccctgtta caaggatgaa aaccttgcgt
     3001 cctaacccct agacgaacgg gccattgttt actttgcaaa gagcattacc tcatgaaagc
     3061 gggtgcaaag gtaaacgata aatttttttc tgcaaaatat ttgctaaaat tttttactta
     3121 aaagtgtatc gaactgaaaa tccggcatta aaccaaatac caggcgactc aataatgtta
     3181 aaaattggca gaatgtccag ccccaagcaa atgggagcat ccttaaaagt atactcaagg
     3241 ccaattacac cgtcggcacc aaggaaaact ttgctaccgt attcgctctc aaatggattc
     3301 tttttatcgg cattggcagt agcaaaatgg gcaccaaatc cgtagtacca ctttaaaccc
     3361 ggggcatcgg gtataggatt gtgaagctga taaagtccgg taataccaat tcctccccac
     3421 ccaaacgaca gaacaccctc aactgctgat ttctgggcaa agaaatgttt aacggtaact
     3481 ccggaggggt agccacctct gaggccaaga ccagttttat agtcctgggc atttgcagct
     3541 gaataagtta caaacactgt agcgagaata aagagtaatc ttttcatatt ataggtattt
     3601 agttagtgca aatatattca tttgacatta aaaagcgtta gtgtttaaca acctcatcag
     3661 ctaaatgttg taaatcggtt ccagcaagat tgcctgaagt cattatcaac aggttgctat
     3721 tgccccaatt gtgctgtaat aggacgtcga caaggttgtt tgcatcagta aaaacagcag
     3781 gatggggatc accaaatcct tgaattacat cgtttgggtc aattggtggc aattttttca
     3841 attccagggc atggggattg aagaaaacgt atgcaacatc agcacccgtg agagttccat
     3901 tatactggcc taaaaaatct tttgtcaggc tactaaaggt gtgtagctca aagcatacca
     3961 ccagttttcg ggcaggatat agctccctaa cagcctcaat ggtagcttta agttttgatg
     4021 gcgaatgagc aaaatccaaa aaaacactgg tgcttgttcc ttgcgctagc agctgcaatc
     4081 gctttgaagc tccttcgaaa gtttcaatgg cattgtagaa ttgtttttca gtaactccaa
     4141 gggtaatgca agccgcttta gcagcggata tgttctgcat attgtgcttt ccgaatacct
     4201 ttaatgggat gcttgtgcca ttgaaatcaa ggctccagat gccgtttgct ataccatgtg
     4261 gatgggtatg gtagggaatt cttttgatat cgttccgttg gctttcttct acaaccctac
     4321 aaacctcagg gtcgtcggca cagtatatga tttttccgtt gggctcaata caatcaataa
     4381 acttagcaaa ctgttcccgg tagatatcaa aagtgggaaa aacgttaata tgatcccatg
     4441 aaattcccga tatgattgca atgtttggac ggtaaacatg aaattttggt cgaggatcga
     4501 taggggatgt aaggtactca tcgccctcaa aaacagcaaa tggcgcatca tcggagaacg
     4561 acaccatatt ctcaaagccc tttagctgag cgccaacgat ataatcgaac ttatagttga
     4621 ggtgctttag cacgtgcatt atcattgatg tgatggtggt tttgccatgg cttccaccca
     4681 caacaatcct tgttttttgt cgcgattgct cgtaaaggaa ttcggggtac gaatagatct
     4741 ttatggcaag ttcttttgcc ctaagcaatt ccggattatc ggctcgtgca tgcataccca
     4801 gcacaatggc ttcaatttca ggggttataa tgcttgggtt ccaaccctga tggctaggca
     4861 aaagaccatg gtttttaaga tgcgaaagcg atggttcaaa aatttcatcg tcggaaccgg
     4921 ttacctggta accttttagc ttcatggcta tggccaagtt gtgcattgcg ctgccgccta
     4981 tagcaataaa atgaactcgc atttcagttt ctgggattaa ggttatggaa atgtgttttt
     5041 taccgtttct tattgttcaa gtaattcttc cattcaatgg tattgccgta aaatacatta
     5101 aaatcaacct taccctgaat tccttttagt cgcccctcgt ggtggtactg ccaaaaggtc
     5161 catgacttct caatttcggg ggtatgatcg aaagagcaga tccagatatc gtgttcggga
     5221 aacgtttcgc tgatgtacca tttaaatgta tccttattgg tgtaaattaa aaccttttgt
     5281 cctgttgttt tttcaacaag ttcaacaaag tcaaccaggt cggcaattat ttgcttgcgt
     5341 ggcctacgaa ccacattacc ccattcctca acatcaacca cagggggtaa atcgagttca
     5401 accccatcaa ttgccctcaa gaagttttgt gcctgagcaa tgcccctccg gttgaagtta
     5461 aagaaatggt aagcacccac ggggataccc gcttcccttg cacgggtaac attgttgcga
     5521 aacgatttgt ccttaagggt ggcaccctcg cttgccttta taaaggcaaa atcaacctta
     5581 tgatccttta ctttttccca gtcaattttt ccggtgtgag ctgatacgtc gataccccaa
     5641 atcaagtaac ggtgatgagg atcggaaatg aa
//
LOCUS       DAOOWJ010000142         5664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_14423_length_5664_cov_8.329470, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000142 DAOOWJ010000000
VERSION     DAOOWJ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14423_length_5664_cov_8.329470"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3467)
                     /gene="cas9"
                     /locus_tag="PLW22_10820"
     CDS             complement(<1..3467)
                     /gene="cas9"
                     /locus_tag="PLW22_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOU75234.1"
                     /translation="MKRILGLDLGSASIGWALISESEINGNVNREILGLGCRIIPYEG
                     TEGKDFEKGTGESRNSIRTKMRTVRKGYDRYQLRRKYLVQELIKHNMYPTDELKGLPK
                     MQLWELRSKAVSEKVSLPELGRILLWLNQKRGYKSSRSDSNLDKKDTEYVAKVKSRHQ
                     LIKEQNLTIGQYFYQQLKNDPFFRVKENVFPREAYIEEFDSICNEQKKHYPDILTDEL
                     ISKIRDEIIYYQRPLKSQKGLVSVCEFEGFWRKGNDGKEYFVGPKVAPKSSPLFQLAK
                     IWENINNIRIENKQGNNVEITLEQKKALFEHLDNNEKLTPTDIIRILGLKKNECFFNK
                     QLAKGINGNTTKCAILNCFDDVSKYAHLLKFDVEVIETGKQTYLYDNSTGEVINEKTI
                     KYIHPKIEKEPLYVLWHTIYSISDQDECSRVLQAKFNIEKEIADKLSGIDFTKQGFGN
                     KSAKTLRKILPYLMEGDGYSEAMCYAGYNHSNSLTKDENLKRELLDKLKPIPKNSLRQ
                     PIVEKILNQMVNVVNAIIDKYGKPDEIRVELARELKQSKDERNETYLNITKRERENEQ
                     IAKKLQEFGLRATRNNILKWRLYEEMDNEDKKLNAICIYCGQPISLVEAIKGTDVDIE
                     HIIPKSKLFDDSQSNKTLAHRHCNSGKGDMTAYDFMKSKPTEVFNAYIERVSKLYANK
                     VISKAKRDKLLMTESKIPENFIDRQLRESQYIAKKAREVLLSISHNVWTTSGSVTAEL
                     RRLWGWDDVTMNLQFNKYKELGLTSFVEWESEHGKNKHRKEVITGWSKRDDHRHHAVD
                     ALVIACTKQGYIQRFNTLNSSKTKQDLIADVEKKSLEFKEKLSLLEKYIISEQPFTVK
                     EVEEKVAEILVSFKAGKKVAVIGKRKVGKKGNKKVVQTGIIIPRGALHEESVYGKVKT
                     VERKPIKYAFENPHLIVLPKIKKLVEERLSKHENVKDAIASLKKEPLYIDKNKQVLLE
                     YASCFSSDYVIKYPVDINFNKVDKVVDGRIKQILQARLNKFNGKPKEAFKDIQRGDRV
                     IKWYEDEGLPKPIKSVRCYTGLSAVVPVKKDENGNNIGFVKPGNNHHIAIYTDSEGKK
                     YEHVCTFWHAVERKKFGFPVIIKNTNEVWDKIQEKPDGTYPEEFLEKLPSPNLTLELS
                     MQQNEMFVLG"
     gene            3952..4554
                     /locus_tag="PLW22_10825"
     CDS             3952..4554
                     /locus_tag="PLW22_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOU75235.1"
                     /translation="MNISKKWIVAGIVILLVVFIYSSIKGTYNNMVKKDEGVKAQWGN
                     VETAYQRRLDLIPNLVNTVKGYANFEQQTLTQVIEARAKATSVNIKPDNLNEQSLKQF
                     QAAQGELSSALARLMVVVEQYPNLKANEQFVNLQAQLEGTENRIAVERRKFNELVQDY
                     NSYIRQFPKNIWASLFGFEKKGYFEAEAGAEKAPKVDFTN"
     gene            4570..5007
                     /locus_tag="PLW22_10830"
     CDS             4570..5007
                     /locus_tag="PLW22_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOU75236.1"
                     /translation="MKPKDYFTPAQQGQIVEAIEQAERQTSGEIRVHVEWECKGSPLD
                     RAARIFSMLKMHKTKLRNGVLFYLSLKDHKFAILGDVGINVKVPENFWDTIKEAMLAE
                     FKEGRLTEGLVKGIGMAGEQLSAHFPYDPNTDTNELSDEISFG"
     gene            5023..>5664
                     /locus_tag="PLW22_10835"
     CDS             5023..>5664
                     /locus_tag="PLW22_10835"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOU75237.1"
                     /translation="MKRILYTILSLLALTVPVKAQDIPDPMSPPRLVNDFAGILSADE
                     QNALEQKLRAYNDTTSTQIYVVTVKDLNGYEPSDFAFRLGEKWGVGQKGKNNGIVILI
                     KPKIGNDKGRAFIATGYGMEEVIPDVTARQIVDKEMIPYFKENRYYKGIDAAINVIID
                     LAAGLYTADEYYSGESPWPGIIFIIIFFAIIFLSARRGNRLQGGSIGRSLPFWL"
BASE COUNT         1546 a         1222 c         1050 g         1846 t
ORIGIN      
        1 ccaaggacaa acatctcatt ctgctgcatg ctcaactcca gggtcaggtt aggcgatggt
       61 agtttttcaa ggaactcttc ggggtaagtt ccatcaggtt tttcctggat tttatcccaa
      121 acctcatttg tatttttgat aattacaggg aacccgaatt tctttcgctc aacggcgtgc
      181 cagaacgtgc aaacatgctc atacttttta ccttcggaat cggtatatat tgcaatgtga
      241 tgattgtttc cgggttttac gaaaccaata ttgttgccat tctcatcctt ttttacagga
      301 actacggctg agaggccagt gtagcaacga actgatttta ttggttttgg caggccttca
      361 tcttcatacc acttaatcac cctatcgcca cgctgtatgt ccttaaaagc ctctttcggt
      421 ttaccattaa atttgttaag ccttgcttgc agtatctgtt taatcctccc atcaacaact
      481 ttatcaacct tattgaaatt tatatcaacc ggatatttta tcacatagtc gctgctaaag
      541 catgaagcgt attctagcaa aacctgttta tttttatcaa tatacaaagg ctcttttttc
      601 aacgaggcaa tggcatcctt tacgttttca tgttttgaaa gacgttcctc aactagtttt
      661 tttatttttg gaagcacaat taaatggggg ttttcaaacg cgtacttaat tggcttgcgt
      721 tcaacagtct taaccttccc atacacactc tcttcgtgta atgctcctct tggaataata
      781 atgcctgttt gaacaacctt tttattaccc tttttcccga ccttcctttt accaataacc
      841 gcaacctttt tgcctgcctt aaacgatacc aaaatttctg caactttttc ctcaacctcc
      901 ttaactgtaa agggctgttc gctaattata tatttttcaa ggagcgagag cttttcttta
      961 aattctaaag attttttctc aacatcggct atcaggtctt gttttgtttt gcttgagtta
     1021 agggtgttaa atcgctgaat ataaccctgt tttgtgcatg caataacaag tgcatctact
     1081 gcgtgatgcc gatggtcgtc gcgtttactc catcctgtaa taacttcctt cctgtgctta
     1141 ttcttgccat gctcgctttc ccactccaca aacgatgtca agcctaactc cttatatttg
     1201 ttaaattgaa gattcatagt aacatcgtcc caaccccata atctcctgag ttcagcagta
     1261 acgctacctg aagtagtcca tacattatgt gaaattgata gaagtacttc ccttgccttt
     1321 tttgcaatat actggctttc ccttaactgt cggtcaataa agttttctgg tattttgctt
     1381 tcggtcataa gcagtttgtc gcgcttagcc ttgctaataa ctttatttgc gtacagcttc
     1441 gataccctct caatgtaggc attaaaaacc tcagtgggtt ttgatttcat aaaatcgtaa
     1501 gcagtcatat cgcctttacc agaattgcag tggcgatgtg caagggtttt attgctttgt
     1561 gaatcgtcaa agagtttgga ttttggaatt atatgttcaa tatcaacgtc agtgccctta
     1621 attgcctcaa ccaaagagat tggttggcca cagtaaatac aaattgcgtt taacttctta
     1681 tcctcattgt ccatttcctc ataaagacgc catttaagaa tattgtttcg ggttgctctt
     1741 agcccaaact cttgcagttt cttagctatt tgttcattct ccctttcccg ctttgtaata
     1801 ttcaaataag tttcgttacg ttcatcctta ctttgcttta gctctctggc taattcaacc
     1861 ctaatctcat cgggtttccc gtatttatca ataattgcat tgacaacatt aaccatttgg
     1921 ttcagtattt tttccacaat gggttgcctg agcgaatttt ttggaatagg ctttaattta
     1981 tccagtaact ctctttttaa attctcatct tttgtaaggg aatttgaatg attataacca
     2041 gcatagcaca tggcttcgct atacccatct ccctccatta ggtatggcaa aatttttctt
     2101 aatgtttttg ccgatttatt gccaaatcct tgttttgtaa aatcgatacc tgagagctta
     2161 tcagctattt ctttttctat gttaaacttg gcctgtaaaa ccctactgca ttcatcttgg
     2221 tcagatattg agtatatggt atgccatagc acatacagtg gctctttctc tatttttgga
     2281 tgaatgtact ttatagtttt ctcgtttatt acctccccgg tggaattatc ataaaggtat
     2341 gtttgtttac cagtttcaat aacctcaaca tcaaacttta aaagatgagc atacttacta
     2401 acatcatcaa aacaatttaa tatagcacac ttggttgtat taccattaat tcccttagcc
     2461 aattgcttat tgaaaaaaca ttcgtttttc ttcaagccta gaatccttat aatatcagta
     2521 ggggtaagtt tttcattatt gtctaaatgt tcaaaaagcg cctttttctg ttcaagggtt
     2581 atttcaacgt tatttccctg tttattttca attctaatgt tattgatgtt ctcccaaatc
     2641 tttgcaagtt gaaaaagagg tgaacttttg ggagcaacct ttggaccaac aaagtattct
     2701 ttgccatcgt tccctttacg ccagaatccc tcaaactcac ataccgacac aagacctttc
     2761 tgggatttta agggtctttg ataatatatt atttcgtccc gaatttttga gattaactca
     2821 tcggttaaaa tatcgggata atgctttttt tgttcattgc aaatactatc aaactcttca
     2881 atataggctt ctcgagggaa tacattctct ttaactctaa aaaacgggtc attctttaat
     2941 tgctggtaaa agtactggcc aatggtaagg ttttgctctt taatcagctg atggcgactt
     3001 ttaacctttg ctacatactc agtatccttt ttatccaaat tagagtcgct tctactactc
     3061 ttgtacccac gcttttgatt cagccacaat agaattctgc ctaactcagg aagcgatacc
     3121 ttttcgctta ctgctttact ccttagttcc cagagctgca ttttgggaag ccctttcagc
     3181 tcatccgttg gatacatatt atgctttatc aactcttgga caaggtattt tctgcggagt
     3241 tgatacctgt cataaccctt gcgtacggtt cgcatttttg ttcggataga gtttctactt
     3301 tcacccgttc ccttttcaaa gtctttccct tcagttcctt cgtaaggaat aatgcgacaa
     3361 cctaaaccca aaatttcgcg atttacatta ccattaattt cgctttcact tattaacgcc
     3421 cagccaattg aagcggaacc taaatcaagt cctaaaattc ttttcataac atttgtattt
     3481 ttaacctaat tagcatatat ttgtattaca atctgaagca attcacaata aggattattc
     3541 cgttgtgaaa acatttaaag cggtctcagt gcaaactggg atcgttttct ttttgggctt
     3601 taaaggtttc gtgaactcca ttgaaaagca ataaaaacgt gaacaaaaca tttaaggcgg
     3661 ggcaactcgc ctttcttttt tatacccttt tctttttccg tttccgctct aaatgcttaa
     3721 cataaaaata ttaaaaatct taataaacaa aaagacataa aatcatatca ttctaaattt
     3781 ttaaaattgc acattctgcc agtatgtcat tttgtgcatt gggtagttac attcccaata
     3841 tatgttaaat cgtactcgtc tttgtgacca gttttaaatc tttaccttaa cttgtgcatg
     3901 atttttgata ccaatgacat cggaattatt aaccttaaaa accatacagc aatgaacatc
     3961 agcaagaaat ggattgtggc aggtattgtt atcctgctgg ttgtctttat ttacagctcc
     4021 attaagggca catacaacaa catggtaaaa aaggatgagg gtgtaaaagc gcagtggggc
     4081 aatgtggaaa cagcctacca gcgcaggctc gatcttatcc ccaacctggt aaatacggtt
     4141 aagggctatg ccaactttga gcagcaaacc cttacccagg taattgaggc cagagcaaaa
     4201 gccacaagcg taaacatcaa gcccgataac ctgaacgagc aaagcttaaa gcagttccag
     4261 gcagctcagg gtgagttaag cagtgccttg gctcgcttaa tggttgtggt tgagcagtac
     4321 cctaacctaa aagccaatga gcagttcgtt aacctgcaag ctcagcttga aggcacggag
     4381 aaccgtattg ccgttgagcg tcgtaagttt aatgagttgg ttcaggatta caactcgtac
     4441 atccgccagt tccccaaaaa tatatgggct agcctgtttg gctttgagaa gaagggttac
     4501 tttgaggctg aagctggtgc cgaaaaggct ccaaaggttg atttcaccaa ctagcatcaa
     4561 ttttgaacca tgaaacccaa ggattacttc acccctgccc agcaaggtca aattgttgaa
     4621 gccattgagc aagccgaaag gcaaacctca ggcgaaatca gggtgcacgt ggagtgggaa
     4681 tgcaagggta gcccactgga tagggctgcc cgtatttttt ccatgctcaa aatgcacaag
     4741 acaaagctac gcaatggggt tctattttac ctatcgctca aggaccacaa gtttgccata
     4801 ctgggcgatg tgggcataaa cgtaaaagtg cccgaaaact tttgggacac tataaaggaa
     4861 gccatgctgg ccgagtttaa ggagggtcgc cttaccgagg gtttggttaa gggaattgga
     4921 atggctggcg agcagctatc ggctcacttc ccgtatgacc ccaacaccga taccaacgag
     4981 ctgagcgatg aaatatcgtt tggttaaaaa aatgatttta atatgaagcg aatactttat
     5041 accatactct cgttgttggc tcttacagtg cctgtaaagg cacaggatat ccccgacccc
     5101 atgtcgccac cccggctggt aaacgatttt gccggtatcc tttcggctga tgagcagaac
     5161 gcccttgagc agaagctgcg ggcctataac gacactacat caacccaaat ctatgtggta
     5221 accgttaagg acctcaacgg ttacgaacct tcggattttg cttttagact tggcgaaaag
     5281 tggggtgttg gccaaaaggg aaagaacaac ggaatagtta ttctgattaa acctaagatt
     5341 ggtaacgaca aaggccgtgc attcatagcc acaggctatg gcatggagga ggtaataccc
     5401 gatgtaacag cccgacagat tgtagacaag gagatgattc cttactttaa ggagaaccgt
     5461 tactacaaag ggattgatgc tgccattaac gtgattattg atttggcagc ggggctttac
     5521 accgccgatg agtactacag cggcgaatcg ccttggcctg gcatcatatt catcataatc
     5581 ttttttgcca ttatcttcct atcggcacgt aggggtaata ggctacaggg tggcagtata
     5641 ggaaggagtc tgccattttg gctg
//
LOCUS       DAOOWJ010000143         5497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_14943_length_5497_cov_6.838111, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000143 DAOOWJ010000000
VERSION     DAOOWJ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14943_length_5497_cov_6.838111"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PLW22_10840"
     CDS             <1..176
                     /locus_tag="PLW22_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75238.1"
                     /translation="RETVKALRKDVTAKCYGGDITRKRKLLEKQKKGKKRMRQIGTVE
                     VPQQAFLAVLKMD"
     gene            186..971
                     /locus_tag="PLW22_10845"
     CDS             186..971
                     /locus_tag="PLW22_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75239.1"
                     /translation="MVLQRLRKARRSRQLGLTSGQIGIYNRYVREREGWEKHLGKSRQ
                     AIVDAVEQFKPQSVCILGSGLLLDVPMEELLSRNINITLVDLAHPAQIVNKYSSNPLV
                     KFETHDLTGGLIQYLQTTRLKLLNFFTLVNLVSKANTYASNAELVVSLNLLSQLSDIP
                     MEFLRHKKVLTDWQCIELASTIQQKHLESLLKAKSLLITDIMEEFYDEQGNLTATRPT
                     VHIDLSLLTYVQEWLWEFDSAKTYNEDAITKLRVVSGLVNKKG"
     gene            complement(1011..2246)
                     /locus_tag="PLW22_10850"
     CDS             complement(1011..2246)
                     /locus_tag="PLW22_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOU75240.1"
                     /translation="MAVPSKFKVFTNRLFGAFKDTQQVESKRSALQNEYNEFIEYTES
                     AELKRFRELAQWFKSGEHQRVKAELKKLTYKGSAEFNTEMEFLSLAKSKEVKVYLNKG
                     GEVTPSVSRYLELKSKVESAEFQNRKKYLLSKNKFEQSEVYAKLVEYQKLQNSDKIKW
                     FLSLERDSSKFNEIREWKLEFYDDFDSNAIDHQKWLTKFFWGDALLNKNYSFTTDKQN
                     YTDKNFEVKNSILRIVTRKEKSEGLGWDSKFGFVPKTYDYTSGVINTGHILRLKEGKV
                     EAKIRLSCCPGVTHAFYMVGNTALPEIDIFIKTDIKPNSFTGAYYTQKSNGKISKSIS
                     SIGGVKCSQNFYILGVEWNGNYIVWSINGTPYKIEKNLTPDMPMYLVFASSVNSKIDD
                     SKLPAYMEIDWVRCWSPLK"
     gene            complement(2266..2646)
                     /locus_tag="PLW22_10855"
     CDS             complement(2266..2646)
                     /locus_tag="PLW22_10855"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU75241.1"
                     /translation="MDRVEIEPTMETPKVVLDKDKAIIEFAGNSLPEDVNSFYQPVLE
                     WIDKYIENPNEKTEINMKFDYYNTSSSKMILKILEKFREVHKKGFSVVVNWHYMEDDE
                     DMIEAGEDYAEHLKIPFKMIPIQQ"
     gene            complement(2757..3449)
                     /locus_tag="PLW22_10860"
     CDS             complement(2757..3449)
                     /locus_tag="PLW22_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU75242.1"
                     /translation="MNQRKIRVMLAEDDENLGYLLKEYLQAKEYDVDLYKDGEKAYKG
                     FQNNYYDICILDVMMPLKDGFTLAKEIRMVNPSMPILFLTAKSMKEDVIEGFAIGADD
                     YMTKPFSIEELLMRLEAILRRTRKDANGAEQTLFQIGKYVFDATKQTLTYEDDVRKLT
                     TKESELLKYLCLNRNTLLDRNFALKTIWADDSYFNARSMDVYITKLRKYLANDPTIEI
                     INVRGKGFKMIY"
     gene            complement(3461..5050)
                     /locus_tag="PLW22_10865"
     CDS             complement(3461..5050)
                     /locus_tag="PLW22_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU75243.1"
                     /translation="MRRKLLFILTGVITIASISLVLLQSKWIRIAVQIKEEQFAQTAS
                     LAMERIIDEVEKQETIVQVIDEIKPYYSVNTSGSAQMSYRQDILNKTKSGFRTKQISQ
                     QVFTLNNLDTLKLPAITKNLFDSSLLGNLPVKISSDPTVSNQQQLSVNVNDKLLKKTV
                     FVENIVDRMIRVELPLNERISQEQLDSIIHRELVRKGIDARYEYRVTNEKDSTIYSSP
                     RFKANFRGLVLRDKLFPNDFFARRYFLTLYFPNQKTYLLGSLGPMTFGTLLLTLLIIS
                     IFTITLYIIFKQKRISEIRNDFVSNMTHELKTPISTISLAAQMLNDKSIPHERKNLDY
                     LGSVIADESKRLGLQVEKVLQMAIFEKAKLRLKVKEVDIHEVIKKVTNNFALQLNSQD
                     GVIIHEFNAKSPIIRADEVHITNVINNLLDNATKYRNGNPIINIITKDVSNGIVVAVK
                     DNGIGISRDNLKKIFDQFYRVPSGNIHNVKGFGLGLSYVKKIVEAHGGKIWVESKLGE
                     GSTFSFFMPWSGPAEQSYIPK"
     gene            complement(5169..>5497)
                     /locus_tag="PLW22_10870"
     CDS             complement(5169..>5497)
                     /locus_tag="PLW22_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75244.1"
                     /translation="SQMVWNINQACTFSLTHNTELEDILGFPSFTDRQTFTGRVITII
                     GNKVTGRVLHAKLSNIDFLMEISGDTDEQFIADTIKQLKSIKEIQVITQVQPSLLKLK
                     EPYCAE"
BASE COUNT         1520 a         1150 c         1123 g         1704 t
ORIGIN      
        1 cacgcgaaac tgtaaaggcg cttcgcaagg atgtaaccgc aaagtgttat gggggtgaca
       61 tcacgcgtaa gcgaaagctg ctcgagaaac agaagaaggg aaagaaacgt atgcgccaga
      121 taggtacagt tgaggttccc caacaggctt ttctggcagt tctcaaaatg gattagtgta
      181 acagaatggt tttacaaaga ttacgaaagg ctcgaaggag tcgccagtta gggctaacct
      241 cggggcaaat tggtatttac aatcgatacg ttcgcgagcg tgagggttgg gaaaaacatc
      301 tcggcaaatc gaggcaagcc atagttgacg ctgttgagca atttaaaccc caaagcgttt
      361 gcattctggg aagcggattg ctgctcgatg ttcccatgga agaactgctc tcgcggaata
      421 tcaacataac cctagttgat ttggctcatc ccgcgcaaat agtcaacaag tactcatcca
      481 atccattggt caaatttgaa acacacgacc ttaccggcgg tctaatacaa tacctgcaaa
      541 ccactaggtt gaagctttta aattttttca ccttagtaaa tctggtttcc aaagcaaata
      601 cctatgccag caatgccgaa cttgtggtat cgctgaacct gttgagtcaa cttagcgata
      661 ttcccatgga atttcttagg cataaaaaag ttcttaccga ttggcagtgc attgaattgg
      721 ccagcaccat tcagcaaaag catctggaat cattgttaaa agcgaaatcg cttttgatta
      781 ccgatatcat ggaagaattt tacgatgagc aagggaacct tactgcaacc cgtcctaccg
      841 tccatatcga cttatcgctt ttaacctatg ttcaggagtg gctctgggaa tttgactccg
      901 ccaaaaccta taatgaggat gccatcacaa agctaagggt agtatcggga ttggtaaaca
      961 aaaagggcta accctaaatg gttagcccta tataaataag gtattaattc ctactttaaa
     1021 ggcgaccagc accgtaccca atcgatttcc atataagctg gcaacttaga atcgtctatc
     1081 ttgctgttta cgctcgatgc aaagaccaaa tacataggca tatcgggtgt aagatttttc
     1141 tcaatcttgt atggggttcc gtttatactc caaacaatgt aattgccatt ccactcaacg
     1201 cctagtatat aaaaattttg actgcactta accccgccaa tgctactaat actttttgag
     1261 atttttccat tactcttctg tgtatagtat gcaccggtga atgaatttgg tttgatgtcg
     1321 gttttaataa aaatgtcaat ctcgggcaat gcagtattgc caaccatgta aaacgcatgg
     1381 gtaacaccag ggcagcatga taggcgaatt ttagcctcaa ctttcccttc ctttaacctt
     1441 aatatgtgac cagtatttat aacgcccgaa gtgtaatcgt aagttttggg aacaaagcca
     1501 aactttgaat cccaaccaag tccctcagat ttctcctttc gggtcacaat tcgtaaaata
     1561 ctattcttta cctcaaagtt cttatcggta tagttctgct tatcggtggt aaatgaatag
     1621 ttcttgttaa ggagcgcatc gccccaaaag aactttgtaa gccatttttg atgatcaatg
     1681 gcattggaat caaaatcgtc gtagaattca agtttccatt ctctaatctc attgaacttg
     1741 ctgctgtcgc gctctaagct aagaaaccat ttaattttat cggaattttg aagcttttgg
     1801 tactcaacca acttagcgta aacctcgctt tgttcaaact tattctttga taaaaggtat
     1861 tttttgcgat tttgaaattc agctgattcc accttgcttt ttagctcaag ataccttgaa
     1921 acggaaggtg taacctcacc ccctttattc aggtaaacct taacctcctt ggatttagct
     1981 aaggaaagaa actccatttc ggtattgaac tctgcggagc ccttatatgt caacttttta
     2041 agttcggcct taaccctctg atgctcacca ctcttaaacc actgggctag ttcgcggaag
     2101 cgttttagct cagccgattc ggtgtattca ataaactcat tgtactcgtt ctgaagagcg
     2161 cttctttttg actcaacctg ttgggtatcc ttaaatgcgc caaaaaggcg attggtaaaa
     2221 actttaaatt tcgagggaac tgccattggt ttaaggttta gggtattact gttgaattgg
     2281 tatcatttta aagggaatct tcaggtgttc agcataatcc tctcccgcct caatcatatc
     2341 ctcatcgtcc tccatgtaat gccagttcac tactactgaa aaaccctttt tatgaacttc
     2401 gcggaatttt tcgagaatct tcaggatcat ctttgatgag gatgtattgt agtagtcgaa
     2461 cttcatgtta atttcggttt tctcattagg attttcaatg tacttgtcta tccactccag
     2521 aaccggctga taaaatgagt taacatcctc aggcaaagag tttcctgcaa attcaataat
     2581 cgctttatct ttatcgagaa caacctttgg ggtttccatg gttggctcta tctcaactct
     2641 atccatatca attatggctt tttatactta ataaccataa agttattaaa tatttattta
     2701 aaaccaaacc ttaccaatac aaaaaaacga aaaaaagttg aatatattga ctgcagctag
     2761 taaatcattt taaacccttt accacgaacg ttaataattt caatggttgg atcgttggca
     2821 aggtatttac gtaactttgt aatataaaca tccatgctcc gagcgttaaa gtagctatcg
     2881 tcggcccaaa tggttttaag ggcaaagtta cggtcaagca gggtgttacg gtttaggcaa
     2941 agatacttga gcaattcgct ttccttggta gtaagtttac gtacatcatc ctcgtaggta
     3001 agggtttgct tggtggcatc gaaaacgtac ttgccaatct ggaaaagggt ttgctctgcc
     3061 ccgttggcat ccttgcgtgt acgacgtagg atagcctcaa gcctcatgag cagctcctca
     3121 atgctaaagg gtttggtcat gtaatcgtcc gcgccaatgg caaatccttc aattacatcc
     3181 tccttcatgg atttggcagt aaggaaaaga atgggcatgc tggggtttac cattctgatt
     3241 tctttggcaa gggtaaaacc atcttttaaa ggcatcatca catcaaggat gcagatgtcg
     3301 tagtagttgt tctggaagcc cttgtaagcc ttttcgccat ccttatacag gtcaacatcg
     3361 tattctttag cttgaagata ctccttgagt aggtatccga ggttttcgtc gtcctcagca
     3421 agcattactc taatttttct ttggttcatg gttatactag ttatttaggt atgtacgatt
     3481 gttctgccgg tccgctccag ggcatgaaaa acgaaaaagt actcccctcg cccagcttgc
     3541 tctcaaccca tatttttccg ccatgcgcct ccactatctt tttaacgtag ctaaggccta
     3601 agccaaagcc cttcacgtta tgtatattgc cgcttggaac cctgtaaaac tggtcaaaaa
     3661 tctttttcag gttgtcgcgg ctaatgccaa taccattatc cttaacagca actactattc
     3721 cattagacac atcttttgtg ataatgttta ttatcggatt accattcctg tacttagttg
     3781 cattgtcgag taagttattt atgacattgg tgatgtgtac ctcatcggcc ctaatgatgg
     3841 gcgatttggc gttaaactca tgaataatta caccatcctg cgaattgagc tgaagggcaa
     3901 aattgttagt tacctttttg atgacctcgt gaatatcaac ctccttcacc ttaagcctta
     3961 gcttggcctt ttcaaagatt gccatttgga gcaccttttc cacctgcaaa ccaagtcgct
     4021 tactctcatc ggctataaca ctgccaaggt aatcgaggtt tttacgttca tggggtatgc
     4081 tcttatcgtt aagcatttgc gcggcaagcg atatggtcga tatgggtgtt tttagctcgt
     4141 gggtcatgtt gcttacaaag tcatttctga tttccgatat ccttttctgc ttgaaaataa
     4201 tgtaaagggt gattgtaaat atagaaataa ttaacaaggt gagcaatagg gttccgaatg
     4261 tcatggggcc caatgatcct aacaggtaag ttttttggtt ggggaagtaa agggttaaaa
     4321 agtaacggcg tgcaaagaaa tcgttaggga aaagcttgtc gcgcaggaca agacccctga
     4381 agttggcttt aaaccgagga ctactgtata tggtactatc cttctcgttg gttactcggt
     4441 actcgtaccg ggcatcaatg cccttgcgga caagttcgcg gtgaataatg gaatcgagtt
     4501 gttcctgcga aatcctttcg ttaagtggca gctcaactcg gatcatacgg tcaacaatgt
     4561 tttcaacaaa aacagttttc ttgagcagct tgtcgttgac gttaacgcta agctgttgct
     4621 gattcgatac tgttggatcc gacgaaatct taaccggtaa gttacccagt aagctcgaat
     4681 caaaaaggtt tttggttatt gctggcagct taagggtatc gaggttgttc agcgtaaaaa
     4741 cctgttggct tatttgcttg gttctgaatc cgcttttggt tttattaagg atatcctggc
     4801 ggtacgacat ttgggctgaa ccgctggtgt ttaccgagta gtagggtttt atctcatcaa
     4861 tcacctggac tatggtttcc tgcttttcaa cctcatcaat tatacgttcc attgccagac
     4921 tggctgtttg ggcaaactgc tcctccttga tttgcacagc aatccttatc cacttggact
     4981 gtagtagaac caagcttatt gatgcaatgg tgataacccc tgtaagtatg aatagtaatt
     5041 tcctgcgcat aagagcaaag ttagttttta tgaaatggcg cggacaactc ttaacaaagt
     5101 ttaaccctaa ggggtgtttt aataacaaga ttataaaggt tgctgattat gaagcggtac
     5161 tgatttaact attcggcgca gtagggttcc tttagtttta ggagtgaggg ttgcacctgg
     5221 gttataacct gaatttcttt tattgatttc aactgcttaa tggtatcggc aataaactgt
     5281 tcgtcggtat cgccgcttat ctccattaaa aaatcaatgt ttgatagctt tgcatgaaga
     5341 accctacctg taaccttatt gccaataatg gtaattaccc ttcctgtaaa ggtttgccta
     5401 tcggtaaacg aaggaaaacc taaaatatcc tcaagctcag tgttatgggt tagtgagaat
     5461 gtgcacgcct gattgatatt ccacaccatt tggctaa
//
LOCUS       DAOOWJ010000144         5446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_15105_length_5446_cov_6.958820, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000144 DAOOWJ010000000
VERSION     DAOOWJ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15105_length_5446_cov_6.958820"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLW22_10875"
     CDS             <1..308
                     /locus_tag="PLW22_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOU75245.1"
                     /translation="KAYLPIINSIQPLRNFVKYTHQADLKAIAHCNSWDLTPFQQVIH
                     RGRSVLVLIGPEGDFTPTEVNLALNSGFTGVSLSTSRLRTETAGVVACHTANLINGV"
     gene            complement(513..713)
                     /locus_tag="PLW22_10880"
     CDS             complement(513..713)
                     /locus_tag="PLW22_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75246.1"
                     /translation="MGQKKTFVLRIDPEKFAALEKWAADEFRSTNGQIEWIIDQALRK
                     AGRLKKSTLKEKGNQSNENQEG"
     gene            complement(720..1568)
                     /locus_tag="PLW22_10885"
     CDS             complement(720..1568)
                     /locus_tag="PLW22_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU75247.1"
                     /translation="MEKEFHPKSGYLMLVAWFILVAATISLLITTESPWYALGFLPIV
                     LIMPGFIVVNPNESAVLILFGKYMGTIKRNGFFWTNPFYLKKRISLRARNFNSEPIKV
                     NDKVGNPIMIGLVLVWRVKDTYKASFEVDEYEHFVVVQSDAALRKLAGMFPYDNFEHE
                     EEEITLRAGGEVVNHKLETELIERLNIAGIQVIEARINYIAYASEIAGAMLRRQQATA
                     VVAARSKIVEGAVGMVQMALDQLSQKQIVDLDEEKKAAMVSNLMVVLCSDESARPVVN
                     AGTLHQ"
     gene            complement(1661..1849)
                     /locus_tag="PLW22_10890"
     CDS             complement(1661..1849)
                     /locus_tag="PLW22_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75248.1"
                     /translation="MKNNNIDSKSRNRPSRKDIEGFNVLEQSNIERYFTDYLEKLERA
                     TPPAPEKQFYQLLAKIKD"
     gene            complement(2516..>2699)
                     /locus_tag="PLW22_10895"
     CDS             complement(2516..>2699)
                     /locus_tag="PLW22_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75249.1"
                     /translation="WQFHPRGTHRVGLLCIDFFGSFLYQDKKERENRSLQNLSVAMQR
                     FSVALRYMHSVHRFRV"
     assembly_gap    2700..2799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3054..4511)
                     /locus_tag="PLW22_10900"
     CDS             complement(3054..4511)
                     /locus_tag="PLW22_10900"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOU75250.1"
                     /translation="MGKSKLKPKELYLLKTKGSFDIAAICREANIILRSQSVTKGELL
                     QYIINAVDTPEVIDTDNRFKTLTQYLKAKNITSVDSKSNELHGEVSLHKPIGYTIYGA
                     DGIEPGAIEQMENAMSLPVTLAGSLMADAHEGYGLPIGGVLATTGNVIIPYAVGVDIA
                     CRMCMSVFPIKPEILGTKSEMLSQILLRTTIFGLGSKNKNHIDTSLFDKKEWSATKVI
                     KQLRDLAYSQLGTSGGGNHFVEWGELHITEFDPLLGFEPGKYLALLSHSGSRGFGSEI
                     ALYYSKLAMEKVRLPGKARHLAWLDLNSQEGQEYWLAMNLAGEYASANHHEIHAKVAR
                     ELNVRPIRMIENHHNFAWFESLPDGRKAIVHRKGATPAHPNSLGIIPGSMVHPAFVIK
                     GKGNPASLSSASHGAGRKMSRSKALQTFTQADLNYILEKWGVELIGAGLDEVPMAYKD
                     IDAVMAYQSDLVGVLAKFYPKIVRMANPERKHRWE"
     gene            complement(4709..5308)
                     /locus_tag="PLW22_10905"
     CDS             complement(4709..5308)
                     /locus_tag="PLW22_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU75251.1"
                     /translation="MNTTSRSDCELLSDFVAGNNSALELLYKRYERKVYTYILLMVRK
                     PQLAEDILQETFIKAIKSVKEGKYSETNRFGSWLMRIAHNLVIDQFRQNKQYRVVSNN
                     DFPMDIFSTPRFAEATVEQRLVYERVLYEVRALIDFLPDDQREVVMLRFYGDLSFKEI
                     AEITNVSINTALGRMRYALINLRKMVKEKNLSLEFEMAW"
BASE COUNT         1504 a         1214 c         1096 g         1532 t
ORIGIN      
        1 taaaggccta cctgccaata attaatagta ttcaaccgtt aagaaacttc gttaaatata
       61 cgcatcaagc cgatttaaaa gcaatagctc attgtaatag ctgggatttg actccatttc
      121 agcaggtaat ccataggggt aggagtgttc tagttctcat tggccccgaa ggcgatttca
      181 caccaaccga agtaaacctt gcgcttaata gtggattcac gggtgtaagc ctaagcacat
      241 caaggctacg aacggaaacg gcaggcgttg tagcctgcca taccgcaaac ctaatcaatg
      301 gcgtataacc ttatcccaca aataagtcga gcacattaag tgctttactc atcggaacct
      361 gaaaaaattc cgtgtatttg cagcttaaac aggtaaaggt tgtgaatttt cgattctgaa
      421 tgttaaaaag tttagtggca acactcccag ccataaaaac ttcgcccatt tcaaagtctt
      481 tacagccaca ttttgggcat ggttgcttgg ctctaaccct cttggttttc attgctttgg
      541 ttcccctttt cttttaaggt tgatttttta agccttccgg ctttccttag tgcttggtca
      601 ataatccatt ctatttgccc attggtgcta cgaaattcgt ctgctgccca tttttctagg
      661 gcagcaaact tttcgggatc gatccgtaac acaaatgttt tcttttggcc catggtttat
      721 tactggtgaa gtgttcccgc atttacaact ggcctagccg attcatcgga gcatagcaca
      781 accatcaagt tgctaaccat tgctgctttc ttctcctcgt caaggtcaac aatttgtttc
      841 tgcgatagct ggtcaagcgc catttgaacc attcctacag caccctcaac aattttgctt
      901 cgggctgcca ctactgccgt tgcttgctgg cggcgaagca ttgcccctgc aatctccgat
      961 gcgtaagcta tatagttaat tctggcctca atcacctgaa tgcccgctat gtttagcctt
     1021 tcaattagct cggtctcaag tttatggtta acaacctctc cgcctgcacg tagggtaatt
     1081 tcttcctcct cgtgttcaaa gttgtcgtag gggaacattc ctgccagctt acggagtgca
     1141 gcatcgctct gcactaccac aaaatgctca tactcatcaa cctcaaatga ggctttgtat
     1201 gtatccttta cgcgccaaac aagcaccaat ccaatcatta tggggttacc aactttgtcg
     1261 ttaaccttaa tgggctcact gttaaagttg cgtgccctaa gcgatattcg ttttttcaga
     1321 tagaaggggt ttgtccagaa aaaaccgttt cgctttatgg tgcccatgta cttcccaaaa
     1381 agtatcagca ctgccgattc gtttgggttt accactataa acccaggcat aatcagcact
     1441 attggcaaaa agccaagagc ataccatggc gattctgttg taatcaacag gctaatggtt
     1501 gctgcaacca gaataaacca agccaccagc atcaggtagc ccgatttggg atgaaattct
     1561 ttttccataa ctgatattat taaagtgata ttaaaatgat attacaaata tattacagaa
     1621 ttatataaaa gtcaataaaa aaggccgatt tttttcggcc ttaatcttta atttttgcta
     1681 aaagttgata gaattgtttt tcgggtgcag gtggtgttgc cctttcgagt ttttccaggt
     1741 aatcagtaaa atacctttca atgttacttt gctcaagtac attaaatcct tcaatgtctt
     1801 ttcgggatgg acggttcctg gatttactat caatattatt atttttcact gcgtagatat
     1861 ttaccatagg caaattggtt gaatggtttc aacatcaaaa cgtatatata tataaggtat
     1921 tgtattaaat cgggaaaaaa ttatgcaaaa tgattcaatg tcgtttagct aacgaaacag
     1981 accctcctta tgtcatccta agggtagcga aggatcaatt caatagtgtg cattgtacag
     2041 tgtgcagttt aggggaagtg tttagtgttt tcactatcaa ctaacaactt tcaactgtca
     2101 acaccacttg tcatcctgag cggcctgtcc cgccctagcg gggagcgaag gatctctgac
     2161 cggccaaatg agatgtttcg ctaacgctca acaaggcaat agttccacga acaacgaaca
     2221 acctttccag ccccgaaggg gcaaagtatt tataactcca ttgtcattcc gagctagtcg
     2281 aggaatctca ttaaataaga gatgaagaca caaagagatg cttcgccttg ctcagcatga
     2341 caatcgcatc atgaaactaa taaatcaagt atcacagagt tgcacaattt aacacgttgt
     2401 ttcacagagt gtttgatatg gtattcgcgg acaacggttc acgaacaacg aacaacgagc
     2461 aacgagcaac gtttccagcc ccgtaggggc gtaatatttg taacttcatg gctactcata
     2521 ctctaaagcg atgcacgcta tgcatatacc taagagcaac actaaaacgt tgcatcgcaa
     2581 cggaaaggtt ttgcaaagag cggttttcac gttctttctt gtcctgatac aagaaagaac
     2641 caaaaaaatc aatgcataaa agcccgaccc gatgtgtgcc ccgtgggtgg aactgccacn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aagcgatgca cgctatgaca
     2821 ttccaaagag caacactgaa acgttgcatc gcaacagaaa agaatggcaa agagcgatgc
     2881 agaaaaccaa tcaaaaatgg aataaaggaa tgaagaaaca aagagacgct ttgacggagt
     2941 ctatcccgat gagtcgaaac tctgcatgac agagagatgt atcgctagca gcatgccaaa
     3001 taatccaaac taaacaacat tgtaaaaatt ttttcaaact agcgagttct ggactattcc
     3061 cagcgatgtt tccgttccgg gttagccatt cttacaattt tggggtagaa cttggcaagc
     3121 acacctacaa ggtcagattg gtatgccata acagcatcaa tatccttgta tgccattggc
     3181 acctcatcga gccctgcacc tataagttcc actccccatt tctccagaat gtagttaaga
     3241 tcagcctgag taaacgtttg tagcgctttg cttctggaca tttttcggcc tgccccatgc
     3301 gatgccgatg aaagcgatgc cggattaccc ttgcctttta tcacaaaggc cggatgaacc
     3361 attgaacctg gaataattcc cagtgaattt gggtgtgcgg gcgtagcgcc tttccggtga
     3421 acaatggcct tacggccatc agggaggctc tcaaaccatg caaagttatg gtgattttca
     3481 atcatcctta tgggacgtac attcagctca cgggcaacct ttgcatggat ctcatggtgg
     3541 ttagccgatg catattcgcc ggctaggttc atggcaagcc agtattcctg accttcctgg
     3601 ctgttcaaat cgagccaggc caaatggcgg gctttgccgg gtagcctcac cttttccatg
     3661 gcaagttttg aatagtaaag agctatttcg ctaccaaatc cccgcgaccc tgaatgtgat
     3721 agcaatgcca ggtactttcc gggttcaaat ccaagcaacg ggtcgaactc ggtaatgtgc
     3781 aattcgcccc attccacaaa gtggttaccg ccacccgatg ttcctagctg gctgtaagcc
     3841 agatcgcgga gctgctttat tacctttgtt gccgaccatt cctttttgtc aaaaagcgaa
     3901 gtgtcaatat ggtttttatt cttactacct agaccaaaaa tggtagttcg gagtaggatt
     3961 tggcttaaca tttcactttt agtacctaaa atctcaggtt taatcggaaa aaccgacata
     4021 cacatccggc aggcaatatc aaccccaaca gcgtaaggaa taataacgtt tccggttgtg
     4081 gctaaaacac caccaatggg caaaccgtac ccttcgtgag catcggccat tagtgagccc
     4141 gcaagtgtaa cgggcaaact catggcgttt tccatttgtt caattgcccc gggttctata
     4201 ccatcggctc cataaatggt gtagccaatg ggcttatgca atgaaacctc accatgaagt
     4261 tcattgcttt ttgaatcgac actggtaata ttcttagcct taaggtactg ggtaagtgtt
     4321 ttaaaccgat tgtcggtatc aatcacttcg ggagtatcaa cagcgttaat gatgtattgc
     4381 agcagttcac cctttgtaac actttggcta cggagtatta tgttggcttc acggcaaata
     4441 gcagcaatat cgaaactccc tttcgttttc agtaagtaaa gttccttggg ttttaacttg
     4501 ctcttgccca ttttagtagt tttcagtgtt ccgtgtgcag tgttcagatg gaatttaagg
     4561 ataaaggttt aattcaagga taaaggataa aggataaagg ataaagagcc tgccccgcat
     4621 tagcgggaat aaaggtttga ggtttgaggg ttaggattca ccatcaacca tcaaccatca
     4681 accatcaact atcaactatc aactttactt accaagccat ttcgaactca aggctaaggt
     4741 ttttctcttt caccattttc ctcagattga ttagcgcgta acgcatgcgc cctaatgctg
     4801 tgttaatgct aacattggta atctcagcga tctccttaaa gctaaggtca ccataaaagc
     4861 gtagcatgac tacctctcgc tggtcatcgg gcaaaaagtc gataagtgct cgtacttcgt
     4921 acagcacgcg ttcatacacc aagcgttgtt caaccgtggc ttcggcaaaa cgcggtgtgc
     4981 taaaaatatc catcgggaaa tcgttgttgg acactacgcg gtactgcttg ttttgcctga
     5041 actggtcaat aaccaggtta tgcgcaatgc gcatcagcca cgagccaaaa cggttagtct
     5101 cggagtactt accttcctta accgatttaa tggcctttat aaaggtctcc tgaagtatgt
     5161 cctcggccaa ctggggtttt cgtaccatta gcagtatata ggtatatacc ttacgttcat
     5221 accttttata taataattca agggctgagt tattgcccgc aacaaaatcc gacagaagct
     5281 cgcagtcgga acgactagta gtattcacgg ctacctcctt aaatttggtt agcgtgtaga
     5341 gcttctatcg ataggcaaat ggttttggtg gttaaacatt aatgcaataa tataaattat
     5401 tacagtttaa tgcaaatttc ttttaaattt atactaggtt taggtg
//
LOCUS       DAOOWJ010000145         5421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_15199_length_5421_cov_7.729594, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000145 DAOOWJ010000000
VERSION     DAOOWJ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15199_length_5421_cov_7.729594"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1470..2300
                     /gene="lgt"
                     /locus_tag="PLW22_10910"
     CDS             1470..2300
                     /gene="lgt"
                     /locus_tag="PLW22_10910"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU75252.1"
                     /translation="MLAYITWNVNPEIFSIGPFSVRYYGLMWAMAFYIGYVIFNRFVK
                     REHLPEGFLDSLTMYMIVGTVIGARLGHCLFYEPEFYLSNPLEILKIWHGGLASHGAA
                     IGILIALYLFARKHKVPMIYVIDRVVITVAIGGAFIRLGNLFNSEIYGVETTLPWGFI
                     FVRNGEVHPKHPTQLYEALSYTAIFILLYWLYLKADGKLRNGFIFGIFLVLLFGARFL
                     IEYVKEPQVEFEKHMMLNMGQWLSLPFIAAGIGFLIYSYLKPSVFIPQDNVKPKKGNK
                     "
     gene            2570..3619
                     /locus_tag="PLW22_10915"
     CDS             2570..3619
                     /locus_tag="PLW22_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOU75253.1"
                     /translation="MPKSIFDNKSLLITGGSGSFGNAVLRRFLKTNISEIRIFSRDEK
                     KQDDMRRFYNDKRIKFYIGDVRNEGSVRSAMVGVDFVFHAAALKQVPSCEFFPVEALK
                     TNALGTDNVLDAAEKYHVKRVVTLSTDKAVYPINAMGITKALMEKIMIARSRNNDPSK
                     QVFCGTRYGNVMASRGSVIPLFVEQIKAGQPLTITDPNMTRYMMTLDDAVDLVLFAFE
                     HGQPGDIFVQKAPAATIETLAKALLELYHAKNPIKVIGTRHGEKLYETLLTREEMVKA
                     YDMGKYFRIPADNRDLNYGLYFSEGQTDITQIEEYNSHNTRRLDVEGMKELLLKLPLI
                     RKDILNEDNGHIYEP"
     gene            3677..4048
                     /locus_tag="PLW22_10920"
     CDS             3677..4048
                     /locus_tag="PLW22_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOU75254.1"
                     /translation="MEINQITHEIIDSAYKVHSTLGPGLLESTYQACLAYELQKKGFQ
                     VEIEKPVPLIYEEIKLECGYRLDLLVENQVVVELKSVDAFTDVHIAQVLTYLKLANKH
                     IGLLINFNTKSLKDGIKRLVL"
     gene            4114..5256
                     /locus_tag="PLW22_10925"
     CDS             4114..5256
                     /locus_tag="PLW22_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU75255.1"
                     /translation="MIKVGITGQSGFIGTHLYNTLGLYPDRFQRIAFEDSYFDDPIKL
                     NGFVQSCDAIVHLAAMNRHNDPQVIYQTNINLCQKLINACEATRSKPHIIFSSSTQEE
                     RDNLYGKSKREGRLLFDQWARRNHTAFSGFIIPNVFGPFGKPYYNSVVATFCHQLTHN
                     EEPKIDVDGELRLIYVSELVEHFITKIDSVAQSRNAVSHVETVQIPHTATIKVSALLA
                     RLNDFKSNYFDKGIFPNLTDPFERNLFNTFVCYIDHEKFFPRMLELKTDDRGSFVETV
                     KLNSGGQVSFSTTKPGVTRGNHFHTRKAERFAVIKGKAVIELRRIGTDKKLTFELDGD
                     RNPSVVDMPIWFTHNIKNVGNDDVYTIFWISEHFNPNDTDTFFETV"
BASE COUNT         1564 a         1302 c         1150 g         1405 t
ORIGIN      
        1 gggagcaaag gatatcatta ttagtgtgca gtgttcagta tgcagtgtac agcggggtag
       61 tgtttcgttt tttggtgttt cgtgtttagt gaaaatctgc gtaaatcacc ccaatctgtg
      121 ctatctgtgt tctattgaat ttactatcaa ctgtcaacca acaaccatca actaaatagg
      181 tatcacaagg ttacacagtg taacacggtg ttacacagaa tggaattaac gaacaacgct
      241 cccagccccg taggggcgta atatttgtaa ctccatggcc actcccctat gtattgcgat
      301 gcacgcaatg atactccaaa gaataaaact gaaacgttgc atcgcaaaag taaatcattg
      361 caaagagcct tttacatgtt ctttcttgtc ttgatacaag aaagaaccaa agaaaatcaa
      421 ggcataaaag cccgacccat taccgggtcg ggcttttatg cctctgcttg ctttttccgc
      481 tccattcctt ctcagggtaa agtgctgggg tgaggccgtt ttccacattc aacctagatg
      541 acccctctaa atctcccctg agaggggaga ctttagccct gcaataaggt ttccttattg
      601 catctcccct ctcctgtcag gagaggggct ggggtgaggt caattccacg aacaaccttt
      661 ccagccccgt aggggcgtaa catttgtaac tccatggcta ctcccatgtg tattgcgatg
      721 cacgcaatat ttttccaaag agcaacggtg aagctttgca tcgcaactga agagtatgac
      781 gaagaacaat aaaaggcatt tagggcatga aggtacaaag agatgcttcg gctgggctca
      841 gcatgacaaa gtgagcgagg gagcccatca tgttgtcatc ccgagcgtag tcgaggaatc
      901 tcatttaaaa gggaatgagg gttcaaagca atgcttcgac aagctcagca tgacaaagga
      961 gtagaagaga agattgggtt tttgtcttcc cgagcgtagc cgaggaatct cattaaataa
     1021 tggatgaagg tattatgaga tgcttcggcg aagtctatcc ctataaattg gaactcagca
     1081 cgacagcgtg atgtttcgct cgcgcgcaac atgactaata accttaacta aacaacattg
     1141 atttaaaatt tcagatttca gattcaagat tttaggtaaa ctcttatgcc atcaaccatc
     1201 aactaaatcg gtatcacagg gttactcaga gttttcaagg tgtttcacag tgaaaatcgg
     1261 cctaatctgt gtcgtctgcg ttccattacc aaactaaaca ctatacacaa aacactaaac
     1321 acaccttaac taacaaccat caactgtcaa ccatcaacca tcaaccatca ccttacaact
     1381 aaacctgtta acccatacta tacatcaata taagggacaa attttcttca aaaacaattt
     1441 ttggcttaca tttacaaaaa ttttaagcta tgcttgcata cataacctgg aatgtcaatc
     1501 ctgagatatt tagtattggc ccgttctcag tacgatacta cggcctaatg tgggccatgg
     1561 ccttttacat tggctatgtc atttttaacc gctttgttaa gcgtgagcac ctgcccgagg
     1621 gtttcctcga ctcgctaacc atgtacatga ttgtaggcac agtaattgga gcccgcttgg
     1681 gccattgcct tttctacgaa ccggaattct acctcagcaa cccgctcgaa atccttaaga
     1741 tctggcacgg aggcttggcc agccatggcg cggccatagg gatactaatt gccctttacc
     1801 tttttgcccg caagcataag gtccccatga tttacgttat tgatagggtt gtaataaccg
     1861 tagcaattgg tggagccttt attcgcctag gaaacctgtt taattctgaa atctacgggg
     1921 ttgaaaccac actgccctgg ggttttattt ttgtgcggaa tggcgaggta cacccaaaac
     1981 accccacaca gctttacgag gcgctatcat acaccgcaat atttatcctg ctttactggc
     2041 tttacctaaa ggccgatggc aagctgcgta atggtttcat ttttggcatc ttcctcgtcc
     2101 tgctctttgg cgcacgattc ctaattgagt acgtaaaaga accacaggtt gagtttgaaa
     2161 aacacatgat gctaaacatg gggcagtggc taagcttacc ctttattgca gcaggaattg
     2221 gatttttgat ttactcatac cttaaacctt cggtttttat cccacaggac aacgtaaagc
     2281 ctaaaaaagg gaataagtag aaacattaaa aaacgtcgga gccaaagccc gacgtttttg
     2341 tttttcgtaa tactttcaac caaataagta tcacagtgtt ttacagggtt tacacagtgt
     2401 ttcacagtgt aaatccgccc agtctgcgcc atctgtgttc cattccaatt cacttacaac
     2461 catcaactat caaccaacaa ctagcttatc tgcgtcatct gtgttcaatt aaattcacca
     2521 ttaactatca actaaaattg ttaactttga atgaataaac cctaccgata tgcccaaaag
     2581 tattttcgat aacaaatcgt tgctaattac cggtggtagc ggatcgtttg gtaacgcggt
     2641 gctgaggcga ttccttaaaa caaatatttc cgaaatccgt attttctcgc gcgacgagaa
     2701 aaagcaggac gatatgcgcc gtttttacaa cgataagcgt atcaagtttt acataggaga
     2761 tgttcgaaac gagggaagtg ttcgttcagc catggttggg gtcgattttg tgttccatgc
     2821 tgccgcgctt aagcaggttc catcgtgcga gttcttcccg gtggaagccc ttaaaaccaa
     2881 tgcgcttggc accgacaacg tgctcgatgc tgccgaaaag taccatgtta aacgggtggt
     2941 tacccttagc accgataagg ccgtttaccc cataaatgcc atgggcatta ccaaggccct
     3001 aatggaaaag attatgattg cccgttccag gaataacgac ccaagcaaac aggttttttg
     3061 cggaacacgt tacggcaatg taatggcctc gcgcgggtcg gtgatcccac tctttgtgga
     3121 gcaaatcaag gccgggcaac cgctcaccat taccgacccc aacatgaccc gctacatgat
     3181 gaccctcgac gatgccgttg acctggtact gtttgccttt gagcatggcc agcccggcga
     3241 tatctttgtg caaaaggcac ccgcggctac aatcgagacc ctggcaaagg ctctgctgga
     3301 gctttaccat gccaagaatc ccattaaggt aattggaacc cgccacggcg aaaagcttta
     3361 cgaaaccctg ctcacccgcg aggagatggt aaaggcttac gacatgggca agtacttccg
     3421 catccccgcc gataaccgcg acctgaacta tggtctgtac ttcagcgagg gacaaaccga
     3481 tatcacacag attgaggagt acaactcgca caacacccga aggctggatg tggagggaat
     3541 gaaggaactg ctgctaaaac taccccttat ccgaaaggat atactgaacg aggacaatgg
     3601 gcatatatac gaaccttaaa tataattagt agcacagggt ttcactgagt ttaacacagt
     3661 gtagcacagt gtaaaaatgg aaatcaacca gattacacac gaaataatcg attcagccta
     3721 caaggtgcat tctactttag gtcctggcct tcttgaatct acctatcagg cttgcctcgc
     3781 ttatgaacta cagaaaaaag gatttcaggt tgagatagaa aaacctgttc ccttaattta
     3841 cgaggagatt aagcttgagt gtgggtatcg actcgacttg ctggtcgaga accaggttgt
     3901 tgtagaacta aaaagcgtag atgcatttac tgatgtacat attgcacagg ttttaaccta
     3961 ccttaaactt gctaataaac acattggact tctgattaat tttaacacaa aaagtctaaa
     4021 agatggaatt aagcgattag ttttgtaata aacaccgtgt aactctgtgc taacactgtg
     4081 gaacactgtg gaacttaact attaaaaaac atcatgatca aagtaggcat taccggacag
     4141 tcaggtttta ttggcacaca cctgtacaac accctgggac tataccccga caggttccag
     4201 cgcatcgcct ttgaggatag ctacttcgac gatcccatta agctcaacgg ctttgttcag
     4261 tcgtgcgatg ccattgtaca cctggccgcc atgaaccgcc acaacgaccc ccaggtcatt
     4321 taccaaacca acatcaacct ttgccaaaag ctcattaacg cctgcgaggc aacccgctca
     4381 aaaccccaca tcatcttctc ctcatcaacc caggaggagc gcgataacct ctacggtaaa
     4441 tcgaagcgcg aagggcgatt gctttttgat cagtgggcca ggcgaaacca tactgccttc
     4501 agcggcttta tcatacccaa cgtgtttgga ccctttggca aaccctacta caactccgtg
     4561 gtggcaacat tctgccacca gcttacgcat aacgaggagc ccaaaattga tgtggatggc
     4621 gagttaaggt tgatttacgt ttcggagctg gtagagcatt ttattaccaa aatcgatagc
     4681 gtagcccaaa gcagaaatgc agtgtcccac gttgaaacgg ttcaaatccc acatacagca
     4741 accatcaagg tttcagccct tttggcaagg ctaaacgatt tcaaatccaa ctacttcgat
     4801 aagggtattt tccccaacct aaccgatccc tttgaacgca acctattcaa cacctttgtt
     4861 tgctacatcg accacgaaaa gttcttcccc cgtatgcttg agctgaaaac cgacgacagg
     4921 ggctcatttg tggagaccgt gaagctgaac agcggcggac aggtttcgtt ctccaccacc
     4981 aagcccggcg ttacccgcgg caaccacttc catacccgca aggcagaaag gtttgccgtg
     5041 ataaagggca aagcagtaat tgagttacgg cgcattggaa ccgataaaaa gcttaccttt
     5101 gagctcgatg gcgatagaaa cccatcggtg gtggatatgc ccatttggtt tacccataac
     5161 atcaaaaacg tggggaacga tgatgtttac accattttct ggatcagtga gcacttcaac
     5221 cctaacgata cggatacgtt ttttgaaact gtttaatgaa tagccgtttc acagagtatc
     5281 acagagtaat gcactgtgta acactgtgaa aacattgtga aacactgtgt aaccagaact
     5341 atgaagaaac ttaaagtcct taccgttgtt ggtacgcgtc ccgagattat ccgcctgtcg
     5401 cgtgtaatgg ccaagctcga c
//
LOCUS       DAOOWJ010000146         5400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_15266_length_5400_cov_8.282694, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000146 DAOOWJ010000000
VERSION     DAOOWJ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15266_length_5400_cov_8.282694"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLW22_10930"
     CDS             <1..600
                     /locus_tag="PLW22_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU75256.1"
                     /translation="QYAAARNVLYANGKKVEIMVNYTPKLHFVTEWWKQLYGESEGKE
                     NKGIFPAGVDFTTDLHSMGQYIQEGERFIFETVLSVEKPWHKLTIPNDPDNLDQLNFL
                     TGKRLTEVNHMAELGTIVAHNDGGVPCIQITIPELSAYWIGYLLYFFERACAISGYTL
                     DVNPFDQPGVEAYKKNMFALLGKPGFEDEGKALRKRLGI"
     gene            complement(667..867)
                     /locus_tag="PLW22_10935"
     CDS             complement(667..867)
                     /locus_tag="PLW22_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75257.1"
                     /translation="MEITRAVLAIGRQWVTNSGFTMDLFIGGLYSKGGIETEEGATGE
                     NFDVAKFEGFRIRTEFSIGFAF"
     gene            complement(1204..3624)
                     /locus_tag="PLW22_10940"
     CDS             complement(1204..3624)
                     /locus_tag="PLW22_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOU75258.1"
                     /translation="MQKRAIYLGLVTVWLLLLTSYSLLFGVPKKYVPMVVYQPDGTKI
                     ECFASGDEFHNWLHDADGYTIIQHPKTGYYVYAAKENGKFVASDFVVGKVKPTATKGL
                     EPFTNISREEYIAKRKRKRVNNHYLSLTPTKGADGIAPLRAKDTKEATVMNNIVIFIR
                     FADSNPDDEPIDYYQNIYNGASMSVSDYYNSVSYGKFTISTTFYPTTTNNYVVWYQDS
                     QNRNYYRPYNATTNPIGYDPNDDNYSSSKSQTYREHTLLKNAIEAVASQIPQNINLDI
                     NEDGYVDVVSFIVAGDNDGWNELLWPHQWSLWSKAVRINQKQVGDYTFQLQLFNNARI
                     DLGTLCHEMFHALGAPDLYHYDDNLNRTPVGVWDIMEGTKDEPQNMGAYMKYLYGGWI
                     DNIPVINQTGTYTLNPLSTSATNNSFIIPSPATHTEYFLVEYRKREAYDASLPNDGML
                     VYRINSKFEGMGNADGPPDEVYVFRPNGSLTLNGTINSATFNADYGRTTMNNQTNPHS
                     FLSDGSNGGINITNVTSAGSTISLNATIDFSPWIVLKNDRGYGSAIGNVSSTLTVATR
                     FTASDMANYVGKYIKKVDFFLRGNDNGQRLTSGEVVKIWEGGTVGNPGTLVYSQGTSA
                     EVKVDEWTTHNVTTPVEIKANKEYWVGYTATASGGYPFATDRGPMVPDKGGWFLNGGQ
                     WVQLAQFNLDYNFLIRAIVSDKTTDVPVNSAEENAISFYPNPAENDGYIKFFNNQNSV
                     NANIEVVNMLGQVIISQSISNLSAGKNVMSVNLSGLSKGIYIVKISMINPASLQVIST
                     KTIKFNKK"
     gene            complement(3664..4203)
                     /locus_tag="PLW22_10945"
     CDS             complement(3664..4203)
                     /locus_tag="PLW22_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75259.1"
                     /translation="MNRIITSGVVFTLLLSCASNTKMEKEQGKNDLVPFVEFYSITEG
                     KALSGVPYQGRRIDGPGYSYDPKTQKLDIYRNNITDSLSIKLYLGVGKVLKGTAGQGV
                     SSTIIGVNEYPFSYNEFTITNATNYKVNCYFKGKQFVLKPNQEYLITETKIDSLPNAT
                     VLQTTTTWKVTFTGFVKNK"
     gene            complement(4325..>5400)
                     /locus_tag="PLW22_10950"
     CDS             complement(4325..>5400)
                     /locus_tag="PLW22_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU75260.1"
                     /translation="LQTINSLIDLPYNNIVRSFIKAYTEKKRDKVEVMLGLTDYYFPM
                     IEEILDMYQLPQELRFLPVIESALNPRAVSRAGATGLWQFMYGTGRMYNLTINSFIDE
                     RRDPLAATHAACRFLKDLYSIYGDWTLVIAAYNCGPGNVNKAIRRSGGKRNYWDIYYH
                     LPRETRGYVPAFIAANYTYYFYKEHNLQPKPITLPPATDTIIVNNMLHLQQIAEVLDY
                     PIELLRDLNPQYKIDIIPAKGKSFVLRLPKEMVGPFLDREKEIYAYKDSIFFNQKILA
                     NPSKYTASYQHEVPPGSIRIVYRVKKGDVLGSIAQRYNVSVRQLKGWNNLHRDLIREN
                     QRLVIYVPERTATRLGIVQEKKS"
BASE COUNT         1453 a         1100 c         1209 g         1638 t
ORIGIN      
        1 cagtacgcag cagcacgcaa cgtgctttat gccaacggca aaaaggtgga aatcatggta
       61 aactacactc ccaaacttca ttttgtaacc gagtggtgga aacagctgta cggcgaaagc
      121 gagggtaagg agaataaggg tattttccct gccggggtcg attttaccac cgatttgcac
      181 tccatgggac agtacattca ggaaggtgag cgttttattt tcgaaacggt tctatcggtc
      241 gagaagccat ggcataagtt aactattccc aacgatcccg ataaccttga ccagctaaac
      301 ttccttactg gtaaacgcct caccgaggta aatcatatgg ccgaactggg taccattgtc
      361 gcccacaacg acgggggagt gccatgcatt cagattacca ttcctgagct aagcgcatac
      421 tggattggat atttgcttta cttctttgag cgggcatgcg ccattagcgg ctacaccttg
      481 gatgttaatc cattcgacca gccgggtgtg gaagcttaca agaagaacat gtttgccttg
      541 ttaggcaaac cgggttttga ggatgagggg aaagctttgc ggaaacgctt aggaatatag
      601 ttacaaagca gtatttgtag attaaaaaga ggtgctccca agcacctctt ttttaattta
      661 aaatgattag aaagcaaatc cgattgagaa ttcagtacga atacggaaac cttcaaattt
      721 tgccacatca aaattttcgc ccgtagcacc ttcttcggtt tcgataccgc ctttactgta
      781 aaggccacca atgaaaagat ccatggtaaa tcccgagtta gtaacccatt gacggccaat
      841 ggctaaaacc gccctcgtaa tttccatagg ttcctttgtc gttggtttca atgttttcaa
      901 gtttaaaatt ctgatacctg aagtatggag caaggtagaa accatctacg gcattgcttt
      961 tgggataaaa acgtacctca ggggttaggg taagtccaga gaattttgaa tcctcaattt
     1021 ttagctaagg tagccaactt aaaatgttca ccttaaaaac attgggttcc tctgaaacgg
     1081 caggtttttc ctgtgcgaac atgggtaatg ttaacattac caggatgcaa gtgtaattag
     1141 tttttcattt ttaaaatttg gattagttta cacgaccaat ttaataagca ttatgccaga
     1201 ttactatttt ttattgaact tgatagtttt tgtactaata acttgtaagc tggcagggtt
     1261 aatcatactt attttaacta tatagatccc ctttgacaaa cccgataggt taacagacat
     1321 aacatttttg ccagcactca agttgctaat agattgggag atgattacct gtccaagcat
     1381 attaaccact tcgatgtttg cattaacaga gttttggtta ttaaaaaatt taatataccc
     1441 atcgttttca gcagggttgg ggtagaaaga tatggcgttt tcctctgctg aattaactgg
     1501 aacatcggta gttttatcag agactatggc tcgaattaaa aagttgtaat ccagattaaa
     1561 ctgagctagt tgtacccatt ggcctccgtt taggaaccaa cctcctttgt cgggaaccat
     1621 agggcctcta tcggtggcga agggataacc accggaggcg gttgcagtgt agcccaccca
     1681 gtactcctta ttggccttta tttcaactgg tgtagtaaca ttatgggtag tccattcatc
     1741 aaccttaacc tcagctgaag tgccctggga gtataccaag gtgccagggt ttcctactgt
     1801 tccaccttcc caaatcttaa ccacttcacc tgaggtaagc ctttgtccat tatcatttcc
     1861 ccttaggaag aaatcaacct ttttgatgta cttcccaaca tagttggcca tatcgcttgc
     1921 tgtaaagcgt gtggcaacag ttagggtaga agatacatta ccaatggccg aaccataccc
     1981 tctatcgttc tttagcacaa tccagggcga gaaatcgatg gttgcattga gtgaaatggt
     2041 agagcctgcg ctagtaacgt ttgtaatgtt tattccccca ttcgatccgt cggaaaggaa
     2101 actgtgcggg ttggtttggt tgttcatggt ggtacgacca taatcggcat taaatgtggc
     2161 actatttatt gttccgttca atgttagaga gccgtttggt cggaatacat atacttcgtc
     2221 gggtgggcca tcggcatttc ccataccctc gaactttgaa ttgatacggt aaaccaacat
     2281 gccatcgttt ggcaggctgg catcgtaggc ctcgcgcttg cggtactcaa ccagaaaata
     2341 ttcagtatga gtagcggggg agggaattat aaagctattg ttagtggccg atgttgaaag
     2401 cgggtttagc gtgtaggtac ctgtttgatt aattacagga atgttatcaa tccagccacc
     2461 gtaaagatat ttcatgtaag ctcccatgtt ctgtggctca tccttggtac cttccattat
     2521 atcccaaact ccaaccggtg tacgatttaa attatcatcg tagtggtaaa ggtcaggggc
     2581 tccaagggca tgaaacattt cgtggcaaag tgtaccaagg tcgattcgag cgttgttaaa
     2641 gagctgcagc tggaaggtgt agtcacccac ttgcttttga tttattctaa cagctttact
     2701 ccacagactc cattggtgtg gccaaagcag ctcgttccag ccatcgttat ccccagcaac
     2761 aataaaggaa accacatcaa catacccatc ttcatttatg tctaagttga tgttttgggg
     2821 aatctgcgaa gcaactgcct caatggcatt ttttaataag gtgtgttcac ggtaggtttg
     2881 gctcttagag cttgaatagt tatcatcatt ggggtcatag ccaatgggat ttgttgttgc
     2941 attgtaaggg cggtagtagt ttcggttttg agaatcctgg taccacacca catagttgtt
     3001 tgtggttgtt gggtaaaaag tggtgcttat tgtgaatttt ccgtaggaaa cactgttgta
     3061 gtagtcggaa actgacattg atgccccatt atagatgttc tggtagtaat caatgggctc
     3121 atcgtcagga ttactgtcgg caaagcgaat gaaaataacg atgttattca taacggttgc
     3181 ctcctttgta tcctttgccc tcaatggtgc aattccatcg gctcctttgg ttggggttag
     3241 cgataggtag tgattgttga cccttttacg cttgcgcttt gcaatgtact cctcgcgcga
     3301 gatgttggta aatggctcta aaccttttgt tgctgttggt tttactttcc caacaacaaa
     3361 atcgctggct acaaattttc cgttctcttt agcggcataa acatagtagc ccgttttagg
     3421 atgctgaatg atagtgtatc catcggcatc gtgtagccag ttgtggaatt catcaccact
     3481 ggcaaaacat tcaatttttg tcccatcggg ttggtaaact accatgggaa catacttttt
     3541 gggcacccca aaaagcaacg agtacgaagt taaaagcagc aaccatacgg ttactaaacc
     3601 caaataaatg gctcttttct gcataaagtt gatgttactg gttactaata cctgagttat
     3661 tgtttacttg ttttttacaa atcctgtaaa tgttaccttc caagttgtgg tggtttgaag
     3721 caccgtggcg ttgggtaatg agtcaatctt ggtttcggta atcaagtatt cttgattagg
     3781 tttcaataca aactgtttac ctttaaaata acagtttacc ttgtagttgg ttgcatttgt
     3841 aatggtaaac tcgttatagc taaaagggta ttcattgacg ccaattattg tgctcgaaac
     3901 tccttggcca gcagtaccct taagtacttt cccaacccca agataaagtt tgatgcttag
     3961 tgagtcggta atgttgttgc ggtatatatc caatttttga gttttggggt cgtaggagta
     4021 cccagggcca tcaattcttc ttccctggta tggtactccg ctcagggcct taccttcggt
     4081 aattgagtag aactcaacaa agggaacaag gtcattcttt ccctgctcct tttccatttt
     4141 ggtgttcgaa gcacaggata aaagcaaggt gaaaaccaca cccgatgtaa tgattcgatt
     4201 catattaaac tatattgact gcaaagaaaa tattatttat tgggttactc atttaataaa
     4261 ataaagaaaa ggacgaacag caagttttgt tcgtctaacg gcaagaaaga aatgctatac
     4321 tacactacga ttttttttcc tgaacaatgc ccaatcgtgt tgcggttcgc tccggaacgt
     4381 agataaccaa tcgttggttt tccctaataa gatctctatg caggttattc cagcccttga
     4441 gctgcctaac cgagacattg tagcgttggg ctattgagcc caacacatca cccttcttca
     4501 ccctgtaaac aatacgaatt gagcctgggg gaacctcgtg ctgatatgat gcagtatatt
     4561 tagatgggtt cgccaaaatt ttttggttga aaaaaatact gtccttgtag gcgtaaattt
     4621 ctttttccct gtcaaggaag gggccaacca tttctttggg taacctcaat acaaacgatt
     4681 ttcccttagc tggaattata tcgattttgt actgtggatt caaatcgcgg agtagctcaa
     4741 tggggtaatc gagcacctct gcaatctgtt gcaggtgtag catgttgttt acaattattg
     4801 tatcggtagc agggggtagg gtaatgggtt tgggttgaag gttatgttcc ttgtaaaagt
     4861 agtaggtgta gttggctgcg atgaaagcgg gaacatatcc gcgggtttcg cggggcaagt
     4921 ggtaatagat atcccagtaa ttccttttgc cgcctgaacg tcggatggct ttgtttacat
     4981 ttccggggcc gcaattataa gccgcaatca caagtgtcca gtctccgtaa attgagtaaa
     5041 ggtctttcag aaatcgacat gctgcatggg tggcagccag cggatcacga cgctcatcga
     5101 tgaaggagtt aatggtaagg ttgtacatcc tgcctgttcc gtacatgaac tgccaaagtc
     5161 cggtagcacc agctcttgaa accgcccggg ggtttaatgc cgattctatt accggtaaaa
     5221 accgaagttc ctgaggtagt tgatacatgt cgagtatttc ctcaatcatg gggaagtaat
     5281 aatcggttaa cccaagcatc acttcaacct tgtcgcgttt tttctccgtg taagctttta
     5341 tgaaactcct aacaatatta ttgtagggta aatcaattaa tgagttgatg gtttgcagac
//
LOCUS       DAOOWJ010000147         5336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_15500_length_5336_cov_5.877296, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000147 DAOOWJ010000000
VERSION     DAOOWJ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15500_length_5336_cov_5.877296"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2171
                     /locus_tag="PLW22_10955"
     CDS             <1..2171
                     /locus_tag="PLW22_10955"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOU75261.1"
                     /translation="VIHVFVDLYRKGYIYRGVRMVNWDPQAKTAVSDEEVIYREVHGK
                     LYHLKYRIDGEDGHVTIATTRPETILGDTAVCVNPNDPRYANLKGKRLLVPLINRSVP
                     VIFDEYVDMEFGTGALKVTPAHDINDYMLGEKYNLEVIDIFNDDGTLNQLAQLYVGMD
                     RFEVRKQIVVDLENAGHLAKVEDYVNKVGFSERTDAVIEPKLSMQWFLKMKDISKPAL
                     DAVVKGDIKLHPPKFVNTYRHWMENVKDWCLSRQLWWGHRIPAWYLPHGGYVVAHNED
                     EAIELARKETGNPNLQPSDLRQDEDVLDTWFSSWLWPISVFNGILDPENRDIKYYYPT
                     HDLITAPEILFFWVARMIIAGYEYMGDKPFRNVYLTGIVRDHLRRKMSKQLGNSPDPL
                     HLIAKYGADGVRVGMLLCSPAGNDLLFDESLTEQGRNFCNKVWNAFRLVKGWQVDANV
                     QQPESARAAVEWFNNVLNKAIEQCDDDFDKYRISEALMILYKLFWDEFSSWYLEAIKP
                     AYGQPIDAVTYSATLEIFEKMLALLHPFMPFITEELWQHLRNRADGESIMVSQMPKST
                     GVDEELIKTFERVKETIAGIRNIRQEKNIAPKNLLTASIRTTSRLNKGFEEIVMKLAG
                     LEQLNYTDGKIDGAASFMVGVDEVYLPLQGLIDVDEERQKILAELEYTRGFLDNVMKK
                     LSNDRFVNNAPTNVVELERKKQSDAETKIKALEERLKELK"
     gene            complement(2430..2708)
                     /locus_tag="PLW22_10960"
     CDS             complement(2430..2708)
                     /locus_tag="PLW22_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75262.1"
                     /translation="MSKICTNDFSINKKIAIGIWAGKEGLFLGSLIDRQNTTILEKGS
                     FHYGIDAKIYIPIVLNPYFGFGIEGGNRTLNNENSFSPNFIARIGLEQ"
     gene            3287..4792
                     /locus_tag="PLW22_10965"
     CDS             3287..4792
                     /locus_tag="PLW22_10965"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOU75263.1"
                     /translation="MNDSEILMNPNDLVRYLKKPASEFTKNDIIRYCEENEIEMVNLR
                     YVVEDGKLKTLNFVISSKDHLDSILSAGERVDGSSLFSFIEAGSSDLYVIPRYRTAFV
                     NPFADVPTIDILCSFYDSTGKPLESAPENVLRKAHDLFKKETGMTFKALAELEYYVIS
                     ERDDFYPGVDQKGYHASEPFAKWENLRREALIFIARAGGKVKYGHSEVGCFTTDEHLY
                     EQHEIEFLPMPAEEAVEQLIIAKWIVRMLGYRYGVDISFAPKITVGKAGSGLHFHMMV
                     EKDGKNMMVENGKLSDTAKRMVAGILDAADALTAFGNTIPISYLRLVPHQEAPTNICW
                     GERNRSVVIRVPLGWIGAEHMVQHANPNDCSNYPKRESKQTVEYRVADGSANPYLTVA
                     GLIVAALDGLRRPDALEMAEKLYVDVNIFKPEFKERLASLKQLPASCWESAEALEAKR
                     QKFEKDGVFPHGMIDSIVTKLKAYNDKGLSEKLYGNNTEIAKLVTQFLHIA"
     gene            complement(4840..5295)
                     /locus_tag="PLW22_10970"
     CDS             complement(4840..5295)
                     /locus_tag="PLW22_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75264.1"
                     /translation="MGKHRNELPITLSNGFKIVWIALIALSLLPTITKVKWYIEASGG
                     SVYNVPMPLRIYQDGRQTIRMTAQFETEPFTLPVYWNIRLGKMIGKRIFETEVIHHKL
                     YLKNTTAETIYFVIGAKSTGALAKVPVNIDHAGFVNVAGQLNFGFGLII"
BASE COUNT         1498 a         1155 c         1218 g         1465 t
ORIGIN      
        1 gtgttatcca tgtttttgtt gacctttacc gtaaaggcta tatttaccgt ggcgtgcgaa
       61 tggtgaactg ggatccgcag gctaaaacag ccgtgtcgga cgaggaggta atttaccgtg
      121 aggttcacgg gaagctttac cacctaaagt acaggattga tggcgaggat ggccatgtaa
      181 ccatagccac tacccgtccc gaaaccattc tgggcgatac tgccgtttgc gtaaatccta
      241 atgatccccg ctacgccaac cttaaaggta aacgcctgtt ggttcctctt atcaatcgca
      301 gcgttccggt aatatttgac gagtatgttg atatggagtt tggtacagga gcactcaagg
      361 ttaccccggc tcacgatatc aacgactaca tgcttggcga gaagtataac cttgaggtta
      421 ttgatatttt caacgatgat ggcaccctga accagttggc gcagctatac gttggaatgg
      481 accgattcga ggtgcgtaaa caaattgttg tcgatctgga gaacgcaggt catttggcta
      541 aggttgagga ctatgtgaat aaggttggct ttagcgaacg tacagatgcc gtgattgagc
      601 ccaagctctc gatgcagtgg ttccttaaaa tgaaggatat ttcaaagcct gctctcgatg
      661 ctgtggttaa aggcgatatc aaattacatc cgcctaagtt tgtgaatacc taccgccact
      721 ggatggagaa tgttaaggat tggtgcctgt cgcgccagct ttggtggggg catcgcattc
      781 ccgcatggta cttgccacat ggcggatatg tggtggcaca caatgaggac gaggccattg
      841 aacttgcccg gaaggagacg ggaaatccta acctgcaacc ttccgattta cgccaggacg
      901 aggacgtgct cgacacatgg ttcagctcat ggctatggcc aatatcggta ttcaacggca
      961 ttctcgaccc cgaaaatcgt gatatcaagt attactaccc cactcacgac ctgataaccg
     1021 caccggaaat cctattcttc tgggttgccc gaatgataat tgccggttat gagtatatgg
     1081 gcgacaagcc tttccgtaat gtttacctta ccggtattgt tcgcgaccac ctgcgtcgca
     1141 agatgagcaa gcaacttggc aactcgcccg acccgcttca ccttatcgca aaatatggtg
     1201 ccgacggtgt tagggttgga atgctccttt gctctcccgc aggtaacgac ttgctttttg
     1261 atgagagcct aacggagcag ggtcgtaact tctgcaataa ggtttggaac gctttccgtt
     1321 tggtaaaggg ctggcaggtt gatgccaatg tccagcaacc cgaatcggca agggctgccg
     1381 ttgagtggtt taacaatgtt cttaacaaag ccattgagca atgcgacgat gattttgata
     1441 agtaccgtat atccgaggca ttgatgattc tgtataaact attctgggat gaattttcat
     1501 cgtggtatct ggaggcaatt aagccagcct acggtcaacc catcgatgca gttacctata
     1561 gcgcaaccct cgaaatcttt gagaaaatgc ttgccttgct tcatcccttt atgccgttca
     1621 ttaccgaaga actttggcaa cacctgcgca atcgcgccga tggcgaaagc attatggtta
     1681 gtcaaatgcc caaatcaaca ggcgttgacg aagaactaat taagaccttt gagcgggtta
     1741 aggaaaccat tgccggaatt cgaaacattc ggcaggagaa gaatatcgca cctaaaaatt
     1801 tgcttacagc atcaattcgc actaccagtc ggttgaacaa aggatttgag gagattgtta
     1861 tgaaacttgc agggttggaa cagctaaact acaccgatgg taagattgat ggagccgctt
     1921 cgttcatggt tggtgttgat gaggtttacc ttcccctcca gggtttaatt gatgttgatg
     1981 aggaacgaca aaaaatcctt gcggaacttg agtacacgcg agggttcctc gataacgtta
     2041 tgaaaaagct cagtaacgat cgatttgtga acaatgctcc tacaaacgtg gtggaacttg
     2101 agcgcaaaaa gcaaagcgat gcagaaacta aaattaaagc gcttgaggaa agattaaagg
     2161 aattaaagta gtttgatttt ctaacctaaa ggttcagtcg cactcgtggt gtaacgggtg
     2221 cgatttttat ttacaagcac aaacgtaatg gttatcgaaa tggctatgca acttattata
     2281 tcctgctcaa tcggttaaat ccagtggaat cgcagccaca ggctgggcat gatatacata
     2341 aaatcggcat acaggcactt gtttagcttc ccatccacaa taaaggtaat gttcagtgcc
     2401 gatcgagtgg aaagctttac gccggcaacc tactgctcaa gtccaatacg tgcaatgaaa
     2461 ttgggcgaaa aagagttctc gttattcaag gttctatttc cgccttcaat cccaaagcca
     2521 aagtatgggt taagtacgat gggtatgtaa atcttggcat ctattccata gtggaaactt
     2581 cctttttcca aaatcgtggt gttttgccta tcgataaggg agcccaggaa caacccttcc
     2641 ttgcctgccc atatgccaat ggctattttc ttgttaatgg aaaagtcgtt cgtgcagatt
     2701 ttgctcaaac ggtaaaattg aatatcaacg ccagttggct caacaatact ggcaccaacc
     2761 ccaacttgcc agcgtcccga atattctttt ttgctgcaac cggtttgttc gcttctgctg
     2821 ggcggacaat gtatttatca gtattatagt agctagaaca gttatgatta tgggcaagat
     2881 tctatatttc atgtagtgcc agattttaaa aaatttctat ttgaagttaa tagtgcaata
     2941 taacgaaatt atcgtcaaac gcacgagttc aagggaacaa taaaatcaaa atcattagcc
     3001 tactttaact acaatccttt aatggctttt acgaacattg gggaagatac tccaacttgc
     3061 ttaatcgcct aaccgattca aaaattgact cgagatcact ttcagtagaa atatttcaag
     3121 gtaaagtctt tggttttaga aaaatcgtct ggatagttcg tctattgctg aaaatatttc
     3181 tacttcaagg aaaaatgcta taaaatgatg ttaataaaca tagccgcaaa cgaatgcaat
     3241 gtcgattgat tttgtagctt tgacaaaatt taaaatttag ggactcatga acgattctga
     3301 aatcctgatg aaccccaacg atttggttcg ctatctaaag aaacccgcat cggagtttac
     3361 taaaaacgac atcattcgct actgcgagga gaatgaaatt gaaatggtta atttacgata
     3421 tgtggtcgag gatgggaaac tcaaaaccct taactttgtt atatcatcaa aggatcacct
     3481 cgattccatc ctctcggcag gcgaaagggt tgacggtagt agcctattct catttatcga
     3541 ggccggtagt agcgacctgt atgttatacc ccgataccga acagcctttg tgaatccatt
     3601 tgccgatgta cctaccatcg atatactatg ctcgttctac gatagcaccg gtaaacccct
     3661 cgagagtgcc cctgagaatg ttctccgtaa ggcacatgac ttgttcaaga aagagactgg
     3721 catgaccttt aaggcacttg ccgagcttga atactacgtg ataagcgaac gggatgattt
     3781 ctaccctggc gtagatcaaa aagggtacca tgcgtcggag ccctttgcca agtgggaaaa
     3841 tcttaggcgc gaagcgctta tcttcatagc acgtgccgga ggtaaggtca agtatggtca
     3901 ttccgaggtg ggctgcttta caaccgacga gcatctttac gaacagcatg agattgaatt
     3961 cctccccatg ccagctgagg aagcagttga acagcttata attgccaagt ggattgtacg
     4021 catgcttggc taccggtatg gtgtagatat tagctttgcc ccaaaaatca cagtaggaaa
     4081 ggctggcagt ggtttgcatt tccatatgat ggtggaaaag gatggcaaga atatgatggt
     4141 tgaaaatgga aagctaagcg ataccgctaa gaggatggta gcaggcattc tcgatgctgc
     4201 cgatgccctt accgcttttg gcaataccat acccatatcg tacttacgcc ttgtccctca
     4261 ccaggaagca ccaactaaca tttgttgggg tgagcgtaac cgttcagtag taattcgcgt
     4321 acctctgggt tggattggtg ccgagcacat ggttcagcac gcaaatccca acgattgtag
     4381 caattatccc aagagggaaa gcaaacaaac cgttgagtac cgggttgccg atggttcagc
     4441 aaacccttac cttaccgttg caggtcttat tgttgctgca ctcgatggcc ttcgtcgccc
     4501 cgatgcactc gaaatggccg agaaactcta tgttgatgtt aatatcttca agcctgagtt
     4561 caaggaaagg cttgccagcc ttaaacaatt gcctgcatcg tgttgggaat cggccgaagc
     4621 cctcgaagcc aaaaggcaaa agtttgaaaa ggacggcgtc ttccctcatg gaatgatcga
     4681 ttcaattgta actaagctta aagcctataa tgataaggga ttaagcgaaa aactttacgg
     4741 aaacaacact gagattgcca aactagttac ccaattcttg catattgcat aacgataacc
     4801 tgtaatgaat cagtgccgac cctttgtggt cggcattttt taaattatta gcccaaatcc
     4861 aaaattcaat tgccctgcaa cgtttacaaa ccctgcatgg tcaatgttta caggaacttt
     4921 ggctaatgct ccggttgatt ttgctccaat tacaaagtag atagtttcgg cagtagtgtt
     4981 tttcaggtat agcttgtgat gtatcacctc cgtttcaaaa atccttttcc caatcatttt
     5041 tcctagtcga atattccagt aaaccggtaa agtgaaaggc tcggtttcaa attgggctgt
     5101 cattctgatg gtttgccttc catcctgata aatacggagt ggcattggta cattataaac
     5161 gctgccaccc gaggcttcaa tgtaccactt tacttttgta atggttggta atagacttaa
     5221 ggctataagg gctatccaaa caattttaaa gccattgctt aatgtaatgg gcaattcgtt
     5281 ccgatgcttt cccatttcca tacaaatcga tgtcgaagtt cgatctttta tttatc
//
LOCUS       DAOOWJ010000148         5165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_16205_length_5165_cov_7.404697, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000148 DAOOWJ010000000
VERSION     DAOOWJ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16205_length_5165_cov_7.404697"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..214
                     /locus_tag="PLW22_10975"
     CDS             17..214
                     /locus_tag="PLW22_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75265.1"
                     /translation="MQKYKHRNIQLLKDLISVCSEQTNITIATEVTSTDEFIVTKTVR
                     EWKNIPLPDINKKNTVFALLA"
     gene            complement(217..1209)
                     /locus_tag="PLW22_10980"
     CDS             complement(217..1209)
                     /locus_tag="PLW22_10980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU75266.1"
                     /translation="MNRNLLLIIAGLSLSAISCDKDFKDPEGDEMARFNAWIQVNNIP
                     ADAKKPSGLYFVSQAEGNGLTPQKGDWILYSYTEKTLDGLVWFTDNSELIKQYDRFDI
                     NYHYTPVFVKYEKAPVQITGYSRTLLKGVYEGLGYLKEGGKATLYIPYNLGYGTSSAT
                     NGLGYQSLIYDIELHKVIKDPRAYEKSLINDYVAQNYPGLEPINDSIYYIQLTPPTVD
                     TVTLAKDSIAYVYYKGMFLDGFVFDTNIDSVAQKLGRKFSSTDSLKVTVGGNDVIKGF
                     SQALLGMKKGEWGRAIIPSFCGYDSIGNSNIPPFTPLIFDIYFSSKGKASTKPK"
     gene            complement(1261..2157)
                     /locus_tag="PLW22_10985"
     CDS             complement(1261..2157)
                     /locus_tag="PLW22_10985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU75267.1"
                     /translation="MRLTHFFLFSSFLAIVASCDNSLETDKRYAEEKSIESYISSKKW
                     SYTKSNGVYCSPQSQSYGYEVNYGDTILFWYKGYTITSPILVFDSNIKSVAIEAKLDT
                     MVRKFEPLRAVIGSTPLLDGLKYGLLLCRENQKATILFPSNLGFGDSYVGPVMPWSPL
                     AFDVEVIYLNGPGKVNEQNLFRTFNLSEYTLHSSGLYYQQVVDTGIVRPTETDKVFGS
                     YTVKLTGGQEIESFATTTEPITLSNLTPHAVRIGFTLASVGGTVNIIAPSPLAYGKKG
                     TPKVSPYTPVEITVKLDSIKTN"
     gene            complement(2154..3044)
                     /locus_tag="PLW22_10990"
     CDS             complement(2154..3044)
                     /locus_tag="PLW22_10990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU75268.1"
                     /translation="MAMNRLFVPAVMALLTLAACSNKSKLFPGYSVTDSGIHYKLITL
                     GESDTPASISNYVTAVIAYRTINDSLFFQGVRQFQLTAPEYEGSIDECFLMLNTGDSA
                     SFYIQAEPFFTKTLGTSLPRFINPGEFMRVDIKLLEVKTAEEFQKEMEAFMSWINDFG
                     EYEKVILKQYLDGQKVDAQPTESGLYIIHQVKTQRSSVEIGDTVTIHYEGRFLNGKLF
                     DSTRKRGEPFQFVYGQKWQVIPGLEEAIGKMREGEKAMLIVPSHLAFGQQGNSNGMIP
                     AFTSVIFELEILEVKKGLAQ"
     gene            complement(3106..3636)
                     /locus_tag="PLW22_10995"
     CDS             complement(3106..3636)
                     /locus_tag="PLW22_10995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU75269.1"
                     /translation="MVNKINTLLIFLLLLSSCNPSTQNVAEQPKSIPDTKQKLLGINY
                     QLTEKDRVLIKAYIDRHRLTMKENPSGLFVYVYGTGYGEYPKKGDLVNYYYRISLLDG
                     TECYKSEPGKPKSMVVGHGGVEAGLEEGIMLMRKGQKALFILPPHLAHGLVGDMDKIP
                     PRASIVYEVELVDVQH"
     gene            complement(3640..4662)
                     /locus_tag="PLW22_11000"
     CDS             complement(3640..4662)
                     /locus_tag="PLW22_11000"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOU75270.1"
                     /translation="MLQIDKSQLNELKKALSSSKNIVITTHHNPDGDAIGSVLGLYHA
                     FRAKGIDSRMVTPNGFPDFLGWLPDADKIMHYSRDKKDVEDLLSNADLLFCLDFNGFK
                     RVEDMAEVLEKSPAKRVLIDHHPEPESPFHISISYTEVSSTAELVYEVIEQIYGESII
                     NYESAVSLYTGIMTDTGSFSYACSRERTFAIAGKLIAKGVVVEDVQGLVYNNFSENRM
                     RLLGFSLAERMKVYPEHRAACIWLSRNDLNTFKHQIGDTEGFVNYPLSIKGIVFSVLF
                     TENEGFVKVSLRSRGNFPVNTFSKLYYQGGGHKNAAGGKAFMGMDEAVKQFEELIAKH
                     SIELNS"
     gene            4753..>5165
                     /gene="ndk"
                     /locus_tag="PLW22_11005"
     CDS             4753..>5165
                     /gene="ndk"
                     /locus_tag="PLW22_11005"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU75271.1"
                     /translation="MNTRFTFCMIKPDAFKSDTVSDILLDILKAGFKIRGIKITMLTK
                     SKAEQFYDIHQGKEFFDRLTTFMSSGPVMAMVLERPDAVESLRKLIGKTDPAQADEGT
                     IRKKFGSDTTHNAVHAADSYENAEREWSFFFSKRE"
BASE COUNT         1432 a         1091 c         1088 g         1554 t
ORIGIN      
        1 taactaataa aagtccatgc aaaaatataa acaccgtaat attcagctcc taaaggattt
       61 aattagtgtt tgcagtgaac aaacaaacat cactatagct acggaggtaa catcaactga
      121 tgaatttatt gttaccaaaa cagtaaggga gtggaaaaat atcccactcc ccgatatcaa
      181 taagaaaaac actgtatttg ctttactggc ttaaggctat ttgggcttag ttgatgcttt
      241 gcccttgctg ctgaaataaa tgtcaaagat aagtggtgtg aaaggcggta tgttactgtt
      301 gcctatggaa tcgtaaccgc aaaacgatgg gattattgct cgtccccatt cgcctttttt
      361 catgcctaaa agcgcttggc taaacccttt tataacatca ttgcctccca cagtaacttt
      421 tagcgagtcg gttgagctaa actttctgcc aagcttttgg gccacgctat caatgttggt
      481 gtcaaaaaca aatccatcga gaaacattcc cttgtagtaa acataggcaa tagaatcctt
      541 tgcaagtgtt accgtatcaa cggtaggcgg ggttaactga atatagtaga tgctatcgtt
      601 aatgggttca agtccagggt aattctgtgc tacataatca ttaatcaaac ttttttcgta
      661 tgctcttgga tctttgatta ccttatggag ttcaatatcg taaattagcg actggtagcc
      721 caatccgttg gttgcacttg aggttccgta gccaaggtta tacggaatgt aaagggtggc
      781 tttgcctcct tcctttagat aacccaaccc ctcatagacc cctttgagta gtgttctgct
      841 gtatccggtt atttgcacgg gcgctttttc atactttaca aatacaggtg tgtaatgata
      901 gttgatatca aacctgtcgt attgttttat aagctcactg ttatcggtaa accaaactaa
      961 tccatcaagt gttttttcag tataggagta cagaatccaa tcgccttttt gcggcgtaag
     1021 gccattacct tcggcttggc ttacaaagta taaaccactt ggttttttag catcggcggg
     1081 gatattgtta acctgaatcc atgcattaaa acgagccatt tcatcacctt cggggtcttt
     1141 aaaatcctta tcgcacgaaa tggctgaaag tgaaaggcct gcaataatca aaagcaaatt
     1201 tctattcata gtttctattc aatttactgc aaatatacta tttaccaatt caattcaaag
     1261 ttaattggtt ttaatactat caagtttaac agttatttcc accggggtgt agggtgaaac
     1321 ttttggggtt ccttttttcc catacgctaa aggcgatggt gcaataatat ttactgttcc
     1381 acccaccgat gccagggtaa accctatacg aacagcatga ggagttaggt tgctgagcgt
     1441 aattggctcg gttgtggtag caaaactttc aatttcttgt ccgccagtta gtttaactgt
     1501 atagctacca aacaccttgt cggtttctgt tggtcggact ataccggtgt caaccacttg
     1561 ctggtagtag agtccactgg agtgaagcgt atattctgaa agattgaaag ttctaaatag
     1621 attttgctcg tttacctttc cggggccatt caagtagata acctcaacat cgaaagctaa
     1681 tggtgaccat ggcattaccg ggccaacata gctatcgcca aatccgaggt tcgatggaaa
     1741 aaggattgtt gccttttggt tttccctgca aagtaacaag ccgtacttca agccatcgag
     1801 taggggagtt gagcctataa cagcccttag cggttcaaac ttgcgaacca tggtatcaag
     1861 cttagcttca atggcaaccg atttaatatt ggagtcaaaa actaaaatgg gtgaagtaat
     1921 ggtgtagccc ttataccaaa aaagaatggt atcgccatag tttacttcgt aaccatacga
     1981 ttgtgattgg ggtgagcagt aaaccccatt actttttgtg taactccatt ttttactgct
     2041 tatgtagctt tcgatacttt tttcctcagc gtatcgttta tcggtttcca gtgagttgtc
     2101 gcacgaagcc acgatggcca aaaagctcga aaaaagaaaa aaatgcgtta atctcattgt
     2161 gctaatcctt tcttaacctc cagtatttca agttcaaaaa tcaccgatgt aaatgcgggt
     2221 atcataccgt ttgaattacc ctgttgccca aaagcaaggt gcgatggcac aataagcatg
     2281 gccttttcgc cctcgcgcat tttaccaatg gcttcctcca ggcccggaat cacttgccat
     2341 ttttggccgt aaacaaactg gaatggctcg ccccgcttgc gggtggagtc gaaaagtttt
     2401 ccgtttagga aacgtccctc gtagtgtatt gttacggtat cgccaatttc aacgctactg
     2461 cgttgagttt ttacttgatg aattatgtaa agccccgatt cagttggctg tgcatcaact
     2521 ttttggccat cgaggtattg ctttagtata accttttcgt attcgccaaa gtcgtttatc
     2581 cagctcatga atgcttccat ctctttttgg aactcctcgg cagttttaac ctcaagtagt
     2641 ttgatgtcaa ccctcatgaa ttcaccaggg ttgataaacc ttggaagcga tgttccaagg
     2701 gttttggtaa agaatggctc agcttgaatg tagaacgatg cactatcgcc agtatttagc
     2761 atcagaaagc attcgtcaat ggagccttca tactcaggcg cggtaagctg aaactgacgt
     2821 acaccctgga agaaaaggga gtcgtttatg gtgcggtatg caattactgc tgttacgtaa
     2881 ttacttatcg atgcaggggt gtcgctttcg ccaagcgtaa tgagcttgta atgaattcca
     2941 ctatcggtta cgctgtaacc ggggaatagt ttggatttat ttgaacatgc cgcaagggtt
     3001 aaaagtgcca taacggcggg gacaaataag cgattcattg ccatggagtg cagggtttta
     3061 gttttgttta aggttataaa tttcattgta aaaatagaaa atatttcaat gctgaacatc
     3121 aaccagttca acctcataaa ctattgaagc ccgggggggg attttatcca tatcgcctac
     3181 caaaccatgt gctaggtgtg gtggtagaat aaaaagcgct ttttgccctt tccgcatgag
     3241 cataataccc tcctccagtc ctgcttcaac tcctccatgc cccactacca tgcttttagg
     3301 tttgcccggt tctgatttgt agcattctgt cccgtccaga agcgaaatgc ggtagtagta
     3361 attgaccaaa tcaccttttt tggggtattc accgtaaccc gtaccataaa catatacaaa
     3421 tagtccgctt gggttttctt tcatggttaa cctatgccta tcgatatagg cttttattag
     3481 caccctatcc ttctcggtaa gctgatagtt gatacccaaa agcttttgct tggtgtctgg
     3541 aattgatttt ggctgttccg caacattttg tgttgaagga ttgcaggagc tcagcaaaag
     3601 caaaaaaatc aaaagggtat ttattttatt taccatacac tatgagttaa gctctatgga
     3661 atgcttggca attagctcct caaactgttt cacagcctca tccataccca tgaatgcttt
     3721 tccaccggca gcatttttgt gtcctccacc ctggtagtat agctttgaga aagtgtttac
     3781 agggaaatta ccccttgaac gcaacgaaac tttcacgaaa ccttcgtttt cagtgaaaag
     3841 aactgaaaaa acgatgcctt taattgataa tggatagtta acaaaaccct cggtatcgcc
     3901 aatctgatgt ttgaacgtgt ttaggtcgtt tcggctaagc caaatgcaag ctgcacgatg
     3961 ctcagggtaa accttcatgc gttcggcaag agagaagcca agtaggcgca ttcggttttc
     4021 cgaaaagtta ttgtaaacca aaccttgaac atcctcaaca actactcctt tggctattag
     4081 ctttcctgca atggcaaatg tccgctcgcg cgagcatgcg tagctgaacg aacctgtatc
     4141 ggtcataata ccagtgtaga ggcttactgc agattcatag ttaataatac tctcgccata
     4201 aatttgctca ataacctcat aaaccagctc ggcggttgag ctgacctcag tgtatgaaat
     4261 tgatatatgg aaagggcttt cgggttcagg gtgatggtcg ataagaaccc gtttggcggg
     4321 cgatttttcc aatacttcgg ccatatcctc tacccgcttg aatccgttaa agtccaagca
     4381 gaatagcagg tcggcatttg aaagcaaatc ctctacatcc tttttatccc ttgagtagtg
     4441 cataatctta tcggcatcgg gcagccaacc taaaaagtcg ggaaaaccat tgggtgtaac
     4501 catacgtgag tcgataccct ttgccctaaa tgcatggtat aggccaagaa ctgagccaat
     4561 agcatcgcca tcggggttgt gatgtgtggt tattactatg tttttagaac tgcttagcgc
     4621 ctttttcagc tcgttaagtt gacttttatc tatttgaagc atccgttagg ttatttttat
     4681 aaactgttta caaagatatg ttttgagttt tactttacat ttactatttt gggaaaataa
     4741 attaacagcc atatgaatac acggtttact ttttgtatga taaaacccga cgcctttaaa
     4801 agcgacacgg ttagcgatat cttgctcgat atccttaaag ctggctttaa gatccgaggg
     4861 ataaagatta ccatgctcac caagtcaaag gctgagcagt tttacgatat acaccaggga
     4921 aaggagtttt ttgataggct tacaaccttt atgagttcag ggcctgtaat ggcaatggtt
     4981 ttggaacgcc ccgatgcggt tgaatcgctg cggaagctga ttggtaaaac cgacccagcc
     5041 caggccgatg agggaacaat ccggaaaaag ttcgggtccg acacaaccca taatgccgtt
     5101 catgcagccg atagttacga aaatgccgag cgcgagtgga gtttcttctt ctccaagaga
     5161 gaaat
//
LOCUS       DAOOWJ010000149         4920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_17274_length_4920_cov_8.109969, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000149 DAOOWJ010000000
VERSION     DAOOWJ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17274_length_4920_cov_8.109969"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLW22_11010"
     CDS             complement(<1..491)
                     /locus_tag="PLW22_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOU75272.1"
                     /translation="MKRFFALLLLCFLVVGNSFGQFKRPTLLNDPDYDVGKPLRFGFS
                     IGVNVMDFDAVNRTAQFNADGSKYFAEVTHISAGLNVNAIGDLRIARDIHLRFLPGYS
                     FGQRDVNFFKVDADSTVSLATTMKMESNFIDLPVGIKFLSERNSNVRPYLYLGTDVRI
                     DLAA"
     gene            complement(547..1278)
                     /gene="ubiE"
                     /locus_tag="PLW22_11015"
     CDS             complement(547..1278)
                     /gene="ubiE"
                     /locus_tag="PLW22_11015"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOU75273.1"
                     /translation="MGKSPNTVVDKRTSSVAAMFNSIAPSYDFLNHLLSLGIDKLWRR
                     RLVKRLLRNNPREVLDVATGTADLALVMAKKSPTVKIVGIDISESMLAIGKVKVKKWK
                     FLERITLKKASALCIPSPDNHFDAAMVAFGVRNFEDLAQGLMEICRVLKPGGELGVLE
                     FSMPRHWPFNALYRFYFLKFLPWVGGKVSGNRQAYTYLPESVLSFPQGEAFVQILKLA
                     GFNDVEIRVQTFGIATLYIAKKKNQ"
     gene            complement(1271..3499)
                     /gene="purL"
                     /locus_tag="PLW22_11020"
     CDS             complement(1271..3499)
                     /gene="purL"
                     /locus_tag="PLW22_11020"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOU75274.1"
                     /translation="MPNRTVTVETARELGLLPEEFEMIKNYLGRTPNYTELSIYSVMW
                     SEHASYKNSIKWLKTLPRKGSAILAEAGTENAGLVDIGNGLACAFKIESHNHPCAVEP
                     YQGAATGVGGINRDIFTMGARPVAQLNSLRFGNLKLDRTRWLIKGVVKGIGDYGNAFG
                     IPVLAGEVFFDDSFNMNPLVNAMSVGLVEKDKVVSAVSKGKGNPVYIVGSATGKDGIH
                     GATFASADITEDSAEDIPSIQVGDPFQEKILLEATQEVIRTGALIGMQDMGAAGIICS
                     TSEMSEKGGHGMRIDLDKVPLRQKNMEAWEILLSESQERMLMVVQKGREADVEAVLNK
                     WDISYAIIGEVTEGDTLEFYYHGELVAKVPASSLVLGGGAPVYDREYREPVYYKEYKK
                     FDINKVPEPADLIDVARFLVTHPNIASKRWVYEQYDTMIGTGNMTTNFPSDAGVYNLK
                     GTNKALVMTVDCNSRYVKADPMVGTMIAVSEAARNISCTGAKPLAVTNCLNFGNPYNP
                     EVYWQFVQAVKGMGTACEKFDTPVTGGNVSFYNQASIGGKEEAVFPTPTIGMIGLLEN
                     KNHHTTLAFKEKGHMIYLIGKSVNDIASSEYLYSYHGVKQSPAPYFNLEEEYEVQRAV
                     RELIARNLIQSAHDVSDGGLFIALLESAMPRNLGFDITTDAEIRTDAFLFGEAQGRIV
                     VSVSPSRETQFIDFMVASKVPFSTLGHVTKSEIRVDDVSFGFVADLKKIYDSSLEQLI
                     NG"
     gene            3716..4477
                     /locus_tag="PLW22_11025"
     CDS             3716..4477
                     /locus_tag="PLW22_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU75275.1"
                     /translation="MMRIALITGATSGIGRATALRLAMDEFNLIITGRRLERLEELKK
                     EIEVKYKKDVHILNFDIRNLDEVNSAIDSLPERWRKIDVLVNNAGLAVGLNHIQDGVI
                     DDWERMIDTNIKGVLYITRKVAPMMIERGKGHIVNIGSIAGTQVYENGNVYCSTKHAM
                     HALSQGMRQDMLKHNIKVTEIRPGLLETEFSIVRFKGDSEKAKNTYVGLTPLFAEDIA
                     DAIHFVLTRPPHVCINDLEVTPVAQANAYYVNRNV"
     gene            4541..>4920
                     /locus_tag="PLW22_11030"
     CDS             4541..>4920
                     /locus_tag="PLW22_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOU75276.1"
                     /translation="MGIYLIMIIFFVLSFMVSMQLRSKFARYSRMALPRGLSGKEVAE
                     LMLHNHGIYDVKVISVDGELTDHYNPANKTVNLSPDVYHGRSIAAAAVAAHECGHAIQ
                     HAQSYAFLQMRSALVPVVSVASRWV"
BASE COUNT         1418 a         1171 c         1057 g         1274 t
ORIGIN      
        1 gcggctaaat caattctaac gtcagttccc aggtacaagt aggggcggac attactgttg
       61 cgctccgata agaacttaat tccaacaggc aaatcgatga agttcgattc catcttcatg
      121 gttgtagcca gcgaaacggt actgtcggca tcaaccttaa aaaaatttac atcgcgctgg
      181 ccaaaagagt atccaggcag aaaacgtaga tgaatatcgc gggcaatacg gaggtcgcca
      241 atggcattta cgttaagacc tgccgaaata tgggttacct ctgcaaagta ctttgagcca
      301 tcggcattga actgagcggt tcggttaact gcatcaaaat ccattacatt aacgccaata
      361 gagaaaccaa acctgagcgg tttccctacg tcgtagtcgg ggtcgtttag caatgtgggc
      421 cgtttgaatt gaccaaatga gttgccaact accaaaaaac aaagtaatag taatgcaaaa
      481 aaacgtttca agtgcgattc acagttttag taacgactct gattaaacgg taaccagtca
      541 aaaatattat tgatttttct ttttcgcaat ataaagggtg gcaataccaa aggtttgaac
      601 cctgatttcc acatcgttaa aacccgctaa cttaagtatc tggacaaaag cttcaccctg
      661 agggaatgaa agcaccgatt cagggagata ggtatatgcc tgtcggttgc ccgacacctt
      721 tccaccaacc cacggtaaga attttagaaa gtaaaatcga tataaagcat taaacggcca
      781 gtggcggggc atggagaact ccaaaacacc cagttctccg ccaggtttaa gcacacgaca
      841 aatctccatt agtccttggg ctaagtcctc aaagtttcgc acaccaaatg caaccatggc
      901 agcatcgaaa tggttatcgg gtgagggaat acagagcgcc gaggcctttt taagggtaat
      961 gcgctccaga aacttccatt tttttacctt cacctttcca atggcaagca tcgattcaga
     1021 gatatcgata ccaactatct taacagtagg ggattttttt gccattacca gtgcaaggtc
     1081 ggcagttccg gttgccacat ccaaaacctc tctaggattg ttacgtagca acctttttac
     1141 aagccgtcgc ctccaaagct tatcaatgcc aaggctaagc agatggttaa gaaaatcgta
     1201 gcttggcgca atgctattaa acattgccgc aacgctactg gttcgtttat caacaacagt
     1261 atttggactt ttacccattt atcagctgct caagcgagga gtcgtaaatt ttctttaaat
     1321 cggctacaaa gccaaacgaa acatcatcga ctctaatttc cgacttggta acatgaccaa
     1381 gggttgagaa aggcaccttg cttgctacca taaagtcaat aaactgggtt tcgcgcgatg
     1441 gcgataccga aaccacaatt cgcccttggg cctcaccaaa caggaaggca tcggtacgaa
     1501 tttcggcatc ggtagtgata tcgaaaccta aattgcgagg catggcactt tcaagaagcg
     1561 caatgaatag ccctccatcg gaaacatcgt gtgccgattg aatcaggtta cgtgcaataa
     1621 gttcgcgaac cgcacgttga acctcatact cctcctcaag gttaaagtaa ggcgcaggcg
     1681 actgcttgac accgtggtag ctgtacaggt attcggacga tgcaatatcg tttactgact
     1741 taccaatcaa ataaatcata tgacccttct ccttgaaagc cagcgttgta tggtgattct
     1801 tattttcaag taaaccaatc ataccaatgg ttggtgtagg gaaaacggcc tcctccttgc
     1861 ctccaattga tgcttggttg taaaaactaa cattaccacc ggtaacaggg gtgtcgaact
     1921 tttcacaagc agtacccatc ccctttactg cctgaacaaa ctgccagtaa acttcagggt
     1981 tatatgggtt accaaagttt aggcagttag taacagccaa gggtttagca ccagtacagg
     2041 agatgtttcg ggctgcctcg gatactgcaa tcatagttcc aaccatgggg tcggccttaa
     2101 cgtaacgcga attgcagtca acagtcatca caagcgcctt attggtgccc ttcaggttgt
     2161 atacacccgc atcggagggg aagttggtag tcatattccc agtgccaatc atggtgtcgt
     2221 actgctcata aacccatcgt ttcgatgcaa tattggggtg agtaaccaga aaacgggcta
     2281 catcaatcaa atcggcaggt tcaggaactt tattaatatc gaacttttta tactccttgt
     2341 agtaaactgg ttcacggtac tccctatcgt aaacgggagc gccaccccca agaaccaacg
     2401 atgaagcggg aaccttggca accagctctc catggtagta aaactctagg gtatcgccct
     2461 cggtaacctc accaataatg gcgtagctaa tatcccactt attaagtaca gcctcaacat
     2521 cggcctcacg acccttctga accaccatga gcattcgctc ctgcgattcg gacagcaaga
     2581 tttcccaggc ctccatgttc ttctgcctta agggaacctt atccaaatcg attcgcatgc
     2641 catgtccacc tttttcggac atttccgagg tggaacaaat gataccggca gcacccatat
     2701 cctgcatacc aatcagtgca ccagtcctga tcacctcctg ggttgcttcc agcaagattt
     2761 tttcctgaaa ggggtcgcct acctgaattg aagggatatc ctcggctgag tcctcggtta
     2821 tatcggccga tgcgaatgtc gcaccatgaa taccatcctt tccggtggct gaaccaacaa
     2881 tgtaaaccgg attaccctta ccctttgata cagctgaaac caccttgtcc ttctcaacca
     2941 acccaaccga catggcattt accagaggat tcatgttgaa tgaatcgtcg aagaaaactt
     3001 cgcctgccaa cacaggaatt ccaaaggcat tgccataatc accaataccc ttaaccactc
     3061 ccttaattaa ccagcgagtt ctatcgagct taaggttgcc aaatcgtaat gagtttagct
     3121 gggcaaccgg gcgtgcgccc atggtgaaaa tatcgcgatt aatacctcca actcctgtag
     3181 cagcaccctg gtagggttca acagcacaag ggtggttgtg cgattcaatc ttaaatgcac
     3241 atgccaaacc attgccaata tcaaccaaac ccgcattctc cgttccagct tctgccagta
     3301 tagcggaacc tttacggggt aatgttttta gccacttgat tgaattctta tacgatgcgt
     3361 gttccgacca cattaccgag tatatactta actcagtgta gtttggggtg cgtcccaagt
     3421 agttcttaat catctcaaac tcttcaggaa gaagccctag ctcgcgggcg gtttcaacgg
     3481 taactgttcg gtttggcata actttctaat ttttctactt tataaatttc ctgccaaaaa
     3541 tacgaacatt aactcaagta acataagctg aaatcgaaaa aatagcagtt aaacattcat
     3601 tatcgatgtt ttaccaaaaa agttttaaaa aaccttaagc cattgtgtag ttactgactt
     3661 atattctaat tttgagttaa catgaaacgc ttactaacac acctatacta ttgatattat
     3721 gagaattgca ttgataactg gtgctacctc tggcattgga cgagctactg ccctcaggtt
     3781 ggccatggat gagtttaacc ttatcattac aggcagaaga ctcgaaaggc ttgaagagct
     3841 aaaaaaagag attgaagtaa agtataagaa agatgttcat attctgaact ttgatatccg
     3901 taacctggat gaggttaaca gtgccattga ttcgctccct gagcgctgga gaaaaattga
     3961 tgttctggta aacaatgccg gcttggcagt tggacttaac cacatacagg acggagtaat
     4021 tgatgactgg gaacgaatga ttgacactaa tataaaagga gtgctttaca tcacccgtaa
     4081 ggttgccccc atgatgattg aacgggggaa aggccacatt gtaaatattg gttcgattgc
     4141 aggaacacag gtttacgaga acggaaacgt ttactgttcc accaagcatg ctatgcatgc
     4201 actctcgcag ggtatgcggc aggatatgct aaagcataat atcaaggtta ccgaaattcg
     4261 ccccggattg ctggaaaccg agttctccat tgttcgcttt aagggcgata gcgagaaagc
     4321 caaaaacacc tatgttggac taaccccgct atttgccgag gacattgccg atgccattca
     4381 ctttgtgcta actcgcccac cccacgtttg cattaacgat ttagaggtaa caccagtggc
     4441 tcaggctaac gcctactacg tgaatcgaaa cgtgtaagcg atttgtatag ttgatgctta
     4501 actagctgat aggtgaaaag aaacaataaa taaactcgtt atgggaattt acctgataat
     4561 gataatcttt ttcgtgctaa gcttcatggt ttccatgcag cttaggtcga agtttgcgcg
     4621 ttactcgcgc atggccttac ctcggggact tagcggaaag gaggtggccg agcttatgct
     4681 ccacaaccat ggtatttacg atgtaaaagt aatatcggtt gatggtgagc taaccgacca
     4741 ctacaatccg gcaaataaaa ccgtaaacct tagccccgat gtataccacg gacgcagcat
     4801 tgctgctgca gctgtggcag ctcacgaatg cgggcacgcc attcagcatg cccagagcta
     4861 tgctttcctg cagatgcgtt cggctttggt tcctgtcgtg agcgtagcct cgcgttgggt
//
LOCUS       DAOOWJ010000150         4795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_17792_length_4795_cov_10.733122, whole genome shotgun
            sequence.
ACCESSION   DAOOWJ010000150 DAOOWJ010000000
VERSION     DAOOWJ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17792_length_4795_cov_10.733122"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLW22_11035"
     CDS             <1..1103
                     /locus_tag="PLW22_11035"
                     /inference="COORDINATES: protein motif:HMM:NF027724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5017 domain-containing protein"
                     /protein_id="HOU75277.1"
                     /translation="LISSNYTGDLNQIGTATWVDLTSNFTLPETPTSGYGTFANAGQA
                     DISAFKGKKVYIAFLYKGDSRTTANPKVTTTYQIDNVKVSEIKSSFVLSETNIKKYWK
                     VYKFNGTSWATAENKYIAIQADDYALMGKTYITSSESDAYVIPFLKLKYPFALEGDAK
                     VVSFRSESNQTKALAYEYKYSNGNWTKNSYRETRNDQFLNDGTKWFYDPTVYFTPVSA
                     DYQLLVNWVYQNLDRSYGSSYGNDEFYYGASAYYSNWDLRLSKRTQYNIPGFDTGTES
                     EKIALTWQRLEEGAAKLLSLKFPDAVSEISGFPVYYWIYLKVYQNDLKTVTYVGVFQL
                     QSGEFKRVKTAEDAAVSQGKLTQDQVNWNRSN"
     gene            1410..3974
                     /locus_tag="PLW22_11040"
     CDS             1410..3974
                     /locus_tag="PLW22_11040"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOU75278.1"
                     /translation="MSKDISLMEQQAKQKVKTYTFEEAYEASIKYFKGDEMAAKVWVS
                     KYALKDSFGTIYELTPDDMHWRLAREIARIERKYPNPMSEEEVYHLLKDFKYIVPQGG
                     PMTGIGNKFQIASLSNCFVIGNNNADSYGGIMKVDEEQVQLMKRRGGVGHDLSHIRPK
                     GSPVLNSALTSTGVVPFMERYSNSTREVAQDGRRGALMLSISIKHPDAEKFIDAKMEQ
                     GKITGANVSVKIDDEFMRAAIEGKPYVQQYPIDSSNPKFRQEINASELWKKIVHNAWK
                     SAEPGILFWDTIIRESVPDCYADLGYKTVSTNPCGEIPLCPYDSCRLLALNLYSYVDN
                     PFSPQATFNFELFKKHVHAAQRMMDDIIDLELEKIDAILEKIANDPEDDEIKSVERNL
                     WLKIRRKTIEGRRTGIGITAEGDMLAALNLRYGSDDAIDFAVEVHKSLAVEAYRSSVY
                     TAKERGAFPIFNAQREKDNPFINRIKAEDEELYKEMTTYGRRNIALLTIAPTGTTSLM
                     TQTTSGIEPVFMPYYKRRRKVNPNDKNVKVSFVDKVGDSWEEFNVFHHKFLVWMEVNG
                     YNPEEVKNYDDEQLSELVQKSPYYKATSNDVDWLSKVRMQGMIQKWVDHSISVTVNLP
                     EDITEEMVAKVYQTAWESGCKGCTVYREGSRDGVLVTNKKEKEQATSSAFPSKRPPVL
                     ECDVIRFKNSHEDWVAFVGLYDGRPYEIFTGRTEDDVLPIPKAVTKGRIIKIKDENGS
                     RYDFQYVDKYGYTNTIGGLSHMFNKEFWNYAKLISGVLRNGMPIPDVVNLVSSLRLDN
                     ESINSWIAGVERSLRRYIPDGTKAKPGKKCPECGSESLIYQEGCLTCKNCGHSKCG"
BASE COUNT         1427 a          964 c         1083 g         1321 t
ORIGIN      
        1 ttttaatttc ttctaattac actggagacc tcaatcaaat tggtactgca acatgggttg
       61 atttaacctc aaattttaca ttacctgaaa cgccaacgag tggttacgga acatttgcaa
      121 atgctggcca ggcagatatt agtgctttta aagggaaaaa agtttacatt gcctttttat
      181 acaagggcga tagccgtact actgctaacc ccaaagttac tactacttac caaattgata
      241 atgttaaggt ttctgagatc aaatcttcct ttgtattgag tgaaacaaat attaagaagt
      301 attggaaagt ttataagttc aacggcacaa gttgggccac tgctgaaaat aaatacattg
      361 ccatccaggc tgacgattac gcacttatgg gtaaaaccta tataacatcg agcgagtccg
      421 atgcttacgt tatccctttc ctcaagttaa agtatccgtt tgctcttgaa ggtgatgcca
      481 aggtagtatc gttccgttcg gaaagtaacc aaaccaaagc tcttgcttat gaatataagt
      541 actccaatgg taattggaca aaaaattcat atcgtgaaac cagaaatgat cagttcctaa
      601 acgatggcac aaaatggttt tatgatccaa ctgtttattt tacacccgta tcggccgatt
      661 atcaactcct agttaactgg gtttatcaaa atttagatcg tagttatggt agtagttatg
      721 gcaatgatga attttactac ggagccagcg cttactattc aaactgggat ttacgtttat
      781 ctaaacgtac acaatataat atcccaggat ttgatactgg tacagaaagt gaaaagattg
      841 cactaacttg gcagcgactt gaggagggtg cagcaaaact actctcttta aaattccctg
      901 atgcagtttc tgaaatttct ggattcccag tatattactg gatttattta aaggtttatc
      961 agaacgacct aaaaacagtt acctatgttg gtgttttcca attacaatcg ggtgaattca
     1021 aacgtgtcaa gactgcggag gatgcagcag tttcacaggg aaagcttact caggatcagg
     1081 taaactggaa tagaagtaat taattttaat tgaggcatcc aaattgaggg cgaaggcaac
     1141 ttcgccctct tttcttttcc gcattttatc aggctctgcg gtttaatgtg ttgagtctca
     1201 gaaaccctag taatttcaag tgattcaata ttttatcctg ttaataacta tgaacggttg
     1261 ttaatattta caaccgtttt tttttggttc gggtaagccc aattcgataa attcgtaatt
     1321 aaaatttcaa aaacaaaaaa ttgcttaatc aactcacagc caaagctgtg atatggaatt
     1381 tttgtcaata aagtaaaaaa tatataacca tgagtaaaga catttccctg atggaacagc
     1441 aagcaaagca aaaggtaaag acttacacct ttgaggaggc ctatgaggca agcattaagt
     1501 atttcaaagg cgacgagatg gctgcaaagg tttgggtgag taagtacgcc cttaaggact
     1561 cgttcggaac aatttatgag ttaacccccg atgatatgca ttggcggctg gctcgcgaaa
     1621 ttgcacgcat tgagcggaag tacccaaacc ccatgagtga ggaggaggtt taccacttgc
     1681 ttaaggattt taagtacata gttccccagg gtgggccaat gacaggtatt ggaaacaagt
     1741 ttcagattgc atcgctatca aactgttttg taattggtaa taacaatgcc gactcgtacg
     1801 gtggaataat gaaggttgac gaggagcaag ttcaactcat gaagcgccgt ggcggcgtgg
     1861 ggcacgacct ttctcatatt cgccccaagg gcagtccggt gcttaatagt gcacttacat
     1921 caactggagt agttcccttt atggaacgct actccaactc aacccgcgag gtggctcagg
     1981 acggtcgacg tggcgcactt atgctgtcaa tctccatcaa gcatcccgat gccgaaaagt
     2041 ttattgatgc caaaatggag cagggaaaga taactggtgc caatgtttca gtaaagattg
     2101 acgatgagtt catgcgcgcg gccattgagg ggaaacctta cgtgcagcaa tatccaattg
     2161 actcatcaaa tccaaaattc cgtcaggaaa ttaatgctag cgagctttgg aaaaagattg
     2221 tacataacgc ttggaaatcg gctgagcccg gtatactttt ctgggacacc attatcaggg
     2281 aatcagttcc cgattgctat gccgatttgg gctacaaaac cgtatcgacc aatccatgcg
     2341 gtgagattcc cctttgccca tacgatagct gccgcctact ggcgctcaac ctttatagct
     2401 atgttgataa tccattctcc ccccaggcaa ctttcaactt tgagcttttc aagaagcatg
     2461 tgcatgcagc ccagcgcatg atggacgata taattgacct tgagctcgaa aaaattgatg
     2521 ctatacttga aaagatagcc aatgaccctg aggatgatga aattaaatcg gttgagcgga
     2581 acctatggct aaagatccgc aggaaaacca ttgagggacg aagaactggc attggtataa
     2641 ccgctgaggg cgatatgttg gccgctttaa acttacgcta tggctctgac gatgcaatcg
     2701 attttgcggt tgaggttcac aaatcgttag cggtggaagc atatcgttca tcggtttaca
     2761 ccgcaaagga acgtggtgca ttcccaattt tcaatgcaca gcgcgagaag gataatccat
     2821 tcattaatcg cattaaagcg gaagatgagg aattatacaa ggagatgact acttatggac
     2881 gtagaaacat tgccctgcta accattgctc ctaccggaac cacaagcctt atgactcaaa
     2941 ccacatcggg tattgagcca gtttttatgc cttactataa gcgtcgccgt aaggttaacc
     3001 ccaacgataa gaatgttaag gtgtcgtttg ttgataaggt aggcgattca tgggaggagt
     3061 ttaatgtatt ccaccataaa ttcctggttt ggatggaagt gaatggatac aaccccgaag
     3121 aggtaaagaa ttacgacgac gagcaactaa gcgaactggt tcagaaatcg ccctactata
     3181 aggcaacatc caacgatgtt gactggctta gcaaggttcg catgcaaggg atgatccaaa
     3241 agtgggttga ccactccatt agcgttacag ttaacctccc cgaagatatt accgaggaga
     3301 tggttgcaaa ggtttaccaa acagcctggg agagcggatg caaagggtgc actgtttacc
     3361 gcgagggatc acgcgatggc gtacttgtta ccaacaaaaa ggagaaggaa caagctacat
     3421 catcggcatt cccaagcaag cgtccgccag tactggagtg cgatgttatc aggtttaaaa
     3481 atagccatga ggattgggtt gcattcgtgg gactttacga tggccgtccg tatgagattt
     3541 ttactggtag aaccgaggat gatgtgctgc ctatccccaa agcagtaaca aagggtagga
     3601 ttatcaagat aaaggatgaa aacggtagcc gctacgattt ccagtatgtt gataagtatg
     3661 gctatactaa caccattggt ggtctttcgc acatgttcaa caaggagttt tggaactatg
     3721 ccaagctgat atcgggtgta ctccgaaacg gaatgcccat acccgatgtg gttaacctgg
     3781 tttcatcgtt aaggctcgat aacgaaagta ttaactcatg gattgctggt gttgaacgtt
     3841 ccttgcgccg ttatataccc gatggaacaa aggcaaaacc cggcaagaag tgtcctgagt
     3901 gtggttctga gtcgctgatt taccaggagg gttgtttgac ttgtaagaac tgtggacact
     3961 caaaatgcgg atagtatttt atagaactat aaaaaaccgc ctgtataggc ggttttttta
     4021 tggggtaatg tttggattaa agcagtttac cgtatatact ctttctggaa ttatgtgggg
     4081 tgagctgatt caatcatgta ctatacaata atggatgttg gatatttagg gattagtgtc
     4141 gcttagttga aaaaactctc tttgtcatcc tgagtgtagc gaaggaattc attattagtt
     4201 tactgggttc agtgtgcagt gtacagtagg gttggtgttt agtgtttagt gaaaatctgc
     4261 gtaaaccacc ctaatctgta ctatctgtgt tttattgaat tttctatcaa ctgtaaacca
     4321 acaactaaat aggtatcaca gagttacacg atgtaacacg gtgttacaca gagtttacac
     4381 gatgtttctc agtgtaaatc tgcctagtcc gtgtcatctg tgttccatct cagttttcac
     4441 gaacaatgat tcacgaacaa cgtacaacgt ccgctagcca tgtaatgaaa aatcaactta
     4501 gcccagggtt ttaaccctgg gtattgtggt attcaaaata tttggcgatg cacgctatgc
     4561 atttgccaaa gagcaacacc gaatcgttgc atcgcaaagg gaagtcatgg caaagagcta
     4621 tttacacgtt ctttcttgtc ttgatacaag aaagaaccaa agaaaatcaa ggctgaaaag
     4681 cccgacccga tgggtacccc gtgggtggaa ctgccacgcg atactattcg ccacgcctgt
     4741 ggcgtgactc atgtggtatc gcttactaag cccaccgcca cgttccaccc ccgac
//
LOCUS       DAOOWJ010000151         4794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_17801_length_4794_cov_7.736653, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000151 DAOOWJ010000000
VERSION     DAOOWJ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17801_length_4794_cov_7.736653"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLW22_11045"
     CDS             <1..660
                     /locus_tag="PLW22_11045"
                     /inference="COORDINATES: protein motif:HMM:NF036547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU75279.1"
                     /translation="KFATDFAEKYRMPAIALDDEARMVLISYYWPGNVRQLKNVTEQI
                     SIIEQKRVIDSETLLRYLPDYNASRLPTLYHKKGVVDEQTFATEREILYKILYDMRSD
                     MNELKKLVFDLMRNGVVSPEHVRSSPVFQDLGVTHVDPIVSSAPARGTETKSFVKDLN
                     DIQDTEEIQEETLSLEEKEKELIVKALEKHNGRRKWAAMELGISERTLYRKIKEYNIN
                     L"
     gene            663..1178
                     /gene="lptE"
                     /locus_tag="PLW22_11050"
     CDS             663..1178
                     /gene="lptE"
                     /locus_tag="PLW22_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOU75280.1"
                     /translation="MSRKKFIIPLILVFTLISQNSCKVSYSFTGASIPLEAKTYSVQL
                     FQNLASIVNPTLANYITEELKNRFLTQTRLTPVADFGDFAFSGQIVTYDVSPMAIQGD
                     EIAAQNRLTIAVKVKFVNAIDPKQSFDKTFSQYAVFSSSQNFSQVEQALVQEIVEKLI
                     DDIFNAAASNW"
     gene            1182..1982
                     /locus_tag="PLW22_11055"
     CDS             1182..1982
                     /locus_tag="PLW22_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75281.1"
                     /translation="MDKQSFFSLLEKGSKREQINTNELVELVNRYPYFNAARSALLQQ
                     MFISNAEHFKMELPKHALFIPDRRYFAMLISNLQNSSTNIQADDRVEAATANPISEVR
                     EEDKPAPETLPITPKKRGRPRKKIDDTNSTLELLESGEQPTKTIITESKPLQTDDTDI
                     LELIENGNESGIEPVDLIEKFIAEQPTIPRPATPQRGYDTGEVVDISVNSVIEPDELA
                     TETLAEIYALQGYIDKAISVYEKLRLKYPEKSSYFADQIQKLKKQQQG"
     gene            2007..2336
                     /gene="secG"
                     /locus_tag="PLW22_11060"
     CDS             2007..2336
                     /gene="secG"
                     /locus_tag="PLW22_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOU75282.1"
                     /translation="MFTFIMTFVVIVSILLILIVLAQNPKGGGLASNFAGSNQIMGVR
                     RTADFLEKATWTLAIALVVLSFLATITMPKPEVEQKSVIESKVKETQPSALPNFPMPT
                     EQTDSVK"
     gene            2367..2795
                     /locus_tag="PLW22_11065"
     CDS             2367..2795
                     /locus_tag="PLW22_11065"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOU75283.1"
                     /translation="MKKIFFAILLGSTSLWGWAQNVLDSAAANPSIRFTTTEIDYGSI
                     PQNSNGERVFEFTNNGSKPLILTNVAASCGCTATEWPREPIKPNEKGSITVKYNTSIT
                     GTFRKSIRVFTNSSPEPILLIIKGEIKPSHSDNYQKENQK"
     gene            2802..4001
                     /locus_tag="PLW22_11070"
     CDS             2802..4001
                     /locus_tag="PLW22_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU75284.1"
                     /translation="MPKISEKGLAMPASPIRKLVPYAEEAKRKGRKVYHLNIGQPDIP
                     TPQVALDAVKSTDLKVIEYSHSAGIESYRRGLAKYYQGLGIDITHNDMIITTGGSEAI
                     TIAFMTCLNPGDEVIIPEPFYANYNGFAVQAGVVVKPIVSRIENDFALPPVEEFEKLI
                     TPRTKGIVICNPNNPTGYLYSREELEQLATIVKKHDLYLFSDEVYREFCYDGITHYSA
                     MMLKGLENNVILMDSVSKRYSMCGVRIGALITRNKEVIAAAMKFAQARLSPPSFGQIA
                     AEAALNTPAEYFKEVYDEYIKRRNFMVEALNKMDGVYCPKPKGAFYTVVRLPIDDSDK
                     FAQWLLEHFEHKNQTVMLAPATGFYSTPGLGKNEVRIAYVLNVDDLRNAMECLEVALK
                     QYPGRTL"
     gene            complement(4050..4604)
                     /gene="ruvC"
                     /locus_tag="PLW22_11075"
     CDS             complement(4050..4604)
                     /gene="ruvC"
                     /locus_tag="PLW22_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOU75285.1"
                     /translation="MRTILGIDPGTNVLGYGIIRCSGRNNAKLVVLGVIELSKYRDHY
                     TKLKVIYERVQHLVDEYKPNEVAIEAPFYGKNVQSMLKLGRAQGVAMAAVLSRSIPIY
                     EYAPRKIKMAITGVGSASKEQVAAMLKNILGEKAFDSEYLDATDGLAAAVCHFFQGEQ
                     NETESKVSSWEEFIRKNPGRVKDK"
BASE COUNT         1455 a         1034 c         1025 g         1280 t
ORIGIN      
        1 aagtttgcta ccgattttgc tgaaaagtac cggatgccag caattgcact cgacgatgag
       61 gctcgcatgg ttttaatttc atactattgg ccaggaaatg tccgccagct caagaatgta
      121 acagagcaaa tttcgataat tgagcagaaa agggtaatcg attccgaaac gcttttacgt
      181 taccttcctg actataacgc ttcgcgcctg ccaacccttt accacaaaaa gggtgttgtt
      241 gatgagcaaa cctttgccac agagcgggaa atactctaca agatacttta cgacatgcgc
      301 agcgacatga atgagctaaa gaagcttgtg ttcgatctca tgcgcaatgg cgtggtttcg
      361 cctgagcacg ttcgttcatc gccggtattt caggatttag gcgttacgca tgtcgatcca
      421 attgtctcat cggcacctgc tcgtggtacg gaaacaaagt cgtttgttaa ggatttaaat
      481 gatattcagg atactgagga aattcaggag gaaaccctct cgttggagga aaaggagaag
      541 gagttgatag ttaaggcgct tgaaaagcat aatggtcgac gaaaatgggc tgccatggag
      601 ttgggaattt cagaacgcac gctttaccga aaaattaagg agtacaatat taacctttga
      661 ttatgtccag gaaaaagttc attataccgc taattttagt attcacattg atctcgcaga
      721 atagctgcaa ggtgagctat agttttactg gggcatcaat acccctcgag gctaaaacat
      781 atagcgttca gctcttccag aaccttgcaa gcattgtaaa tccaaccttg gccaactata
      841 ttaccgagga gttaaaaaat agatttttaa cccaaacccg tttaacaccg gttgccgatt
      901 ttggcgattt tgccttttcc ggccagattg taacctatga tgtttcgcca atggctattc
      961 agggcgatga gatagctgct caaaaccggc taaccattgc tgttaaggtg aaatttgtta
     1021 acgccattga cccaaaacaa agttttgaca aaacattttc gcaatacgct gtatttagta
     1081 gtagccagaa tttctcccag gtggagcagg cgctggttca ggagatagtt gaaaagctaa
     1141 tcgacgatat ttttaatgca gcggcatcaa actggtgagc catggacaag caaagtttct
     1201 tcagcctact ggaaaagggt agtaaacgag aacaaatcaa cacaaacgag ctggttgagc
     1261 tggtaaacag gtacccttac tttaacgctg cgcgttcagc attactccag caaatgttca
     1321 tttccaatgc tgaacatttc aaaatggagc tgcccaagca tgcccttttt atccctgacc
     1381 gccgatattt tgcaatgcta atctcgaatc ttcaaaactc cagcacaaac attcaagccg
     1441 atgatagggt tgaagcagct accgctaatc ctatttcaga ggtaagggaa gaggataagc
     1501 cagctcccga aactttgcca ataacaccca agaagcgtgg acggccgcgt aagaaaattg
     1561 acgacaccaa tagcaccctt gagttacttg agagcgggga acagccaaca aaaaccatta
     1621 ttactgaaag taagcccttg caaaccgatg atactgacat tcttgaactt attgaaaatg
     1681 gcaatgagtc aggtattgag ccggttgatt taattgagaa gtttattgct gagcaaccca
     1741 ccatcccgcg tcctgcaact ccgcaaaggg gttacgacac cggtgaggtt gtagatatct
     1801 ccgtaaattc cgttattgag cccgacgaac tcgcaacaga aacacttgct gagatttatg
     1861 ccctccaggg gtatattgat aaagctattt cggtttatga gaaattacgt ttgaaatatc
     1921 cagaaaaaag tagttacttt gccgatcaaa ttcaaaagct taaaaagcaa caacaaggat
     1981 agttaaacta taaattacca taagcgatgt ttacatttat aatgacattt gtagtgatag
     2041 tatcaatttt actgatactt atcgttttag cccagaaccc caagggggga gggttggcat
     2101 caaactttgc cggatcgaac cagataatgg gtgtacgccg cactgccgat tttcttgaaa
     2161 aagctacatg gacattggcc attgcactag tagtacttag ctttctggca accatcacca
     2221 tgccaaagcc tgaggttgaa caaaaatcgg ttattgagtc caaggtaaag gaaactcaac
     2281 ccagcgctct accaaacttc ccaatgccaa ctgagcaaac ggatagcgta aagtaagtta
     2341 cgccttttaa aaacattgct tttgcaatga aaaaaatatt ttttgcaata cttttgggct
     2401 caacctcatt atggggctgg gcccaaaatg ttttggacag tgctgctgca aacccttcaa
     2461 tccggtttac cactactgaa attgactacg gatcaattcc acagaattcc aacggcgaac
     2521 gcgtatttga gtttacaaac aacggttcaa aaccattaat acttactaat gtggcagcgt
     2581 catgtgggtg cactgcaact gagtggcccc gggagcccat aaaaccaaat gaaaagggtt
     2641 caatcaccgt taagtacaat acttcaatta ctggaacttt taggaaaagc atcagggtat
     2701 ttaccaattc aagtcctgag ccaatcctac ttatcataaa gggtgaaatt aaaccaagtc
     2761 atagcgataa ttatcaaaag gaaaaccaaa aatagttaac catgccaaag atttcggaaa
     2821 aaggattagc catgccagct tcgcccattc gtaagctggt accctatgca gaggaagcaa
     2881 aacgtaaggg gcgaaaagtt taccatctaa acatcggcca acccgacata cccaccccac
     2941 aggtagcact cgatgctgtt aagagtactg atttgaaggt tattgagtat agccattcgg
     3001 caggtattga aagctacagg agagggcttg ccaagtatta tcaaggttta ggcatcgata
     3061 tcacccacaa cgatatgatt attaccactg gggggtcgga ggccattacc attgccttca
     3121 tgacctgttt gaatccgggt gatgaggtta ttatacctga acctttctat gccaactaca
     3181 acggatttgc cgttcaggca ggcgtagttg ttaaaccaat tgtttccagg atagagaacg
     3241 attttgcgct accccccgtt gaggagtttg agaagcttat tacccctagg actaaaggta
     3301 ttgtaatttg caaccccaat aatccaacgg gttacctcta ctcaagggaa gagctggagc
     3361 agcttgcaac cattgtaaag aaacacgacc tttacctttt ctccgatgag gtttaccgtg
     3421 aattctgcta cgatggcatt actcattact ctgccatgat gcttaagggg cttgagaaca
     3481 atgttatact gatggattcg gtttcaaagc gctatagcat gtgcggagtt cgaataggtg
     3541 cacttattac ccgcaataaa gaggttattg ctgctgccat gaagtttgct caggcacgtc
     3601 tttcgccccc atcgttcggg caaattgctg ccgaagcagc ccttaatact ccagccgagt
     3661 atttcaagga ggtttacgat gagtacatca agcgacgcaa ctttatggta gaggcgctaa
     3721 acaagatgga tggtgtttac tgtccaaagc ctaaaggtgc tttctacaca gttgttcgct
     3781 taccaattga cgatagcgat aagtttgcac agtggctgct tgaacatttt gagcataaaa
     3841 accaaacggt aatgcttgct cctgcaaccg gattttactc aaccccaggt cttgggaaga
     3901 acgaggtgcg tatagcttac gtgcttaatg ttgatgatct ccgcaatgcc atggaatgct
     3961 tggaggttgc actaaagcaa tacccgggta gaacactata aaattttagt acagataaaa
     4021 aaagaccccg attttttcgg ggtcttttac tatttgtctt taactcgtcc cggatttttt
     4081 ctaataaatt cctcccagct ggaaaccttg ctttcggttt cgttctgttc accctggaaa
     4141 aagtggcaaa ccgcggctgc taaaccatcg gttgcatcaa ggtattcact atcaaaagct
     4201 ttttcgccaa gtatattttt aagcatagcc gccacctgct ctttggatgc actacccaca
     4261 cccgtaatgg ccattttgat tttacgcgga gcgtactcat atatgggtat tgagcgtgaa
     4321 agaacagcag ccatggctac tccttgtgcc cgtccaagct ttagcatgct ttgaacattc
     4381 ttaccgtaaa aaggagcctc aatggctacc tcgttaggct tgtattcatc aaccagatgt
     4441 tgcacccgtt catatatcac ctttagcttt gtgtagtgat cgcggtattt tgaaagctca
     4501 ataacaccta acacaaccag tttagcatta tttcgaccgc tacaccgaat tataccataa
     4561 cccaatacat ttgttccggg atcgataccg agtatggtac gcataaatta aaggttaaag
     4621 gttatataat aaaggctaaa ggctaaaggc taaaggataa aagtttaagg ctaaaggata
     4681 aaggttaaag gataaaggtt aaaagttagg gttaaaggat aaagaaaaaa atcaaagata
     4741 taattggatt ccttccatac caatgatttc agttggttac caagcgccaa tttc
//
LOCUS       DAOOWJ010000152         4747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_18015_length_4747_cov_6.949915, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000152 DAOOWJ010000000
VERSION     DAOOWJ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18015_length_4747_cov_6.949915"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..973)
                     /locus_tag="PLW22_11080"
     CDS             complement(11..973)
                     /locus_tag="PLW22_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOU75286.1"
                     /translation="MNSQIKSLAVETIKSEAIAIEKLAEYIDDNFEQTVNEIFNGKGR
                     VIVTGIGKSAIIASKIVATLNSTGTPAIFMHAADAIHGDLGIIQPDDIIICLSKSGNT
                     PEIKVLVPLIKNLGNKVVAIVSNTDSYLAQKADYVLRATVEREACPNNLAPTSSTTAQ
                     LVIGDALAVCLLKLRGFTPADFARFHPGGSLGKKLYMRVSDIFSQNNVPRVHPNDSIP
                     SVIIEISSKRLGATAVLDDNGKLMGIITDGDLRRMLQNNTTIEGLTAKDIMSRTPKTI
                     DPHELAINAFHLMEQYKITQLLVMENDKYLGVIHIHDILREGIV"
     gene            1143..3347
                     /gene="recQ"
                     /locus_tag="PLW22_11085"
     CDS             1143..3347
                     /gene="recQ"
                     /locus_tag="PLW22_11085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOU75287.1"
                     /translation="MKVDASEISLKEYLNKYFGFTTFKGNQEAIIRNVLAGNDTFVLM
                     PTGGGKSLCYQLPALILDGTAIIISPLIALMKNQVDNMRSFVVEDGVAHFLNSSLNKA
                     QIQKVKDDIMAGTTKMLYVAPESLTKEENVQFLRQVKISFYAIDEAHCISEWGHDFRP
                     EYRRIRPIINEIGQAPLIALTATATPKVQHDIQKNLGMLNATVFKSSFNRPNLYYEVR
                     PKVNAVKEIIKYIKNNPGKSGIIYCLSRKKVEEMAEVLKVNGIKALPYHAGMDASTRS
                     ENQDKFLMEEVDVIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEG
                     QCITFYSYKDIQKLEKFMQGKPVSEQEIGKQLLLETVSYAESSMCRRKTLLNYFGEEF
                     TEDNCNSCDNCLNPKTQFDGQDELQMVLETILAVNQKFKADHIVNILTGNVTAAVKSY
                     KHNQLDTFGEGTDKDPMFWNSIIRQALVMKYIDKDIENYGLLMVTQKGKDFLEKPQEV
                     MFTLDQRYEEGDDDEAIGLSPDKAGGGAADDELLNMLKELRKKMAKKLNLPPFVIFQD
                     PSLEDMAIQYPITIDELQNISGVGAGKARRFGKEFVELIKAYVEEKEIIRPQDMVVKS
                     VANKSLLKVFIIQSIDRKMDFEDIAEAKNLEMDELLTEIEAIVNSGTRINIDYYINKV
                     IDEDKQEEIFDYFRNEAQTESIEEAMAALGDDDITEEEIRLMRIKFMSEMGN"
     gene            3366..4256
                     /locus_tag="PLW22_11090"
     CDS             3366..4256
                     /locus_tag="PLW22_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU75288.1"
                     /translation="MHTLLSINKLTKNYGTTEALCNFTLEVVEGQVLGILGPNGSGKT
                     TLLSILMGLRYPTSGHFTWFNTPPNHVPTTRIGALIEVPTFYPYLSLYDNMRISALAK
                     NISYNEIFPAVERVGLTPNLKAKVATFSLGMKQRMAIAQALLGNPKIIVLDEPTNGLD
                     PEGIVEVRNLIKELNAGGTSIIVASHNLDEVQRTCTHVAILKKGHLQAFGGVNELLTS
                     AKVAIVEVRNPENLIDFLNSNPTIEVIERVGNSFKLLVNKSEHQVTLITDFQNHGIAV
                     NSFEIRNLNLEELFITLVRK"
     gene            4268..>4747
                     /locus_tag="PLW22_11095"
     CDS             4268..>4747
                     /locus_tag="PLW22_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75289.1"
                     /translation="MIRAVKAELIKTLGYATFWVLISLHALLFLLVLTIANKATLNFN
                     GVESNILLSKPYTWGTVAWLASWFNLILALLFIILCGNDFLFGTYRRGLLDGFSRNNL
                     FKAKLSLLILIAFYVVVLVTLIALFATPVLSSWENLLTGYGYILVLFIQMLGYFSIAL
                     "
BASE COUNT         1374 a         1058 c         1055 g         1260 t
ORIGIN      
        1 aaaggatagg ttaaactata ccctcgcgaa gtatatcgtg aatgtgaatt acacctaagt
       61 atttatcgtt ctccattacc agcagctggg ttattttgta ctgttccata aggtggaaag
      121 cattaatggc tagctcgtgc ggatcgattg tttttggggt tctcgacatg atatcctttg
      181 ccgtgagtcc ctcaattgtt gtgttattct ggagcatacg gcgcaagtca ccgtcggtaa
      241 ttattcccat gagtttcccg ttatcgtcca gtaccgcggt agctccaagc cttttggatg
      301 aaatttcaat aataactgaa ggtattgaat cgtttgggtg aacccgcggc acattgtttt
      361 gcgagaagat atcggaaacc cgcatgtaaa gctttttccc cagcgagcca ccaggatgga
      421 aacgagcaaa atcagcagga gtaaaccctc gtagttttaa aagacaaaca gccagggcat
      481 cgccaattac tagctgagcc gtggtgctcg aggtgggtgc cagattattg gggcaggctt
      541 cgcgctcaac ggttgcgcgt aaaacatagt cggccttttg ggctaaatac gaatcagtat
      601 ttgaaacaat ggcaactacc ttattcccta aattctttat caaaggtaca agtaccttga
      661 tttccggggt gtttccactc tttgacaggc aaatgataat atcgtcaggt tggataattc
      721 ctaaatcgcc atggatggca tcggcagcat gcatgaatat ggccggtgtg ccagtagagt
      781 taagggttgc aactattttg cttgctatta tggcgctttt gcctataccg gtaacaataa
      841 ccctaccttt tccgttaaat atttcgttaa ccgtttgctc aaagttatcg tcaatgtact
      901 ctgcaagttt ctcaatagca atagcttcgg acttaatggt ttcaacagcc aggcttttta
      961 tttgtgagtt cattgtaaat caatttttac tacttttgta atcctttgtt aaaagacgct
     1021 aaactaaacc ttaatttaaa tttaatagta tatcttagag caaaagtatt gtttttttat
     1081 ttgatagtta aaacaaacct attaacaaaa gttaaatacc tacccagggg aaacgcaagg
     1141 aaatgaaagt tgatgcatcg gaaatctctt taaaagagta cctgaacaag tatttcgggt
     1201 ttaccacctt caaaggtaac caggaagcta tcattaggaa tgttcttgcc ggaaacgaca
     1261 cctttgtact tatgcctaca ggcggaggta aatcgttatg ctaccagctt ccggcgctca
     1321 tactggatgg tacggccata atcatatcgc cactcatcgc cctgatgaag aatcaggtag
     1381 ataatatgcg tagctttgta gttgaagatg gtgtggcgca ttttcttaac tcatcgctaa
     1441 acaaggcgca gatacagaag gtaaaggacg atataatggc aggaaccacc aagatgcttt
     1501 acgtggctcc cgagtcgctc accaaggagg agaatgttca attcctccgt caggtaaaaa
     1561 tctcgttcta tgccattgac gaggctcact gtatctcgga atgggggcac gattttaggc
     1621 cagaataccg ccgtattcgt cccattatta acgagatagg tcaagcccca ttaattgccc
     1681 ttaccgccac agcaacacca aaggttcaac acgatattca gaagaacctg ggcatgctga
     1741 atgccacggt tttcaaatcg tcgttcaaca ggccaaacct gtactatgag gtacgcccca
     1801 aggttaacgc cgttaaggag ataatcaagt acatcaagaa taacccagga aaatcgggta
     1861 ttatatactg ccttagccgc aaaaaggttg aggagatggc tgaggtgctc aaggttaacg
     1921 gtataaaggc actcccctac catgccggta tggatgccag cacccgttcc gagaaccagg
     1981 ataaattcct gatggaagag gttgatgtta ttgtggctac cattgccttt ggaatgggta
     2041 tcgataagcc agatgttcga tttgttatcc actacgacat acccaaaagc cttgagggtt
     2101 actaccagga aaccggccgc gccggtcgcg acggcggcga gggacaatgt atcacctttt
     2161 acagctacaa ggatattcag aagctggaaa agttcatgca gggtaaacct gtatcggaac
     2221 aggaaatagg caaacagcta ctgctcgaaa ccgtatcgta cgccgagtcg tcgatgtgtc
     2281 gccgcaaaac cttgctaaac tactttggcg aggagttcac tgaggataat tgcaactcat
     2341 gcgataactg ccttaacccc aaaacccagt tcgacgggca ggatgagctt caaatggttc
     2401 tggaaaccat acttgccgtt aaccaaaagt tcaaggccga ccatatagta aacatcctta
     2461 cgggaaatgt tactgctgcc gttaaatcgt ataagcataa ccagctcgac acctttggcg
     2521 aagggactga caaagacccc atgttctgga actccataat ccgccaagcc ttggtaatga
     2581 agtacatcga taaggatatt gagaactacg gtttgctaat ggttacccaa aagggtaagg
     2641 atttccttga gaaacctcag gaggttatgt ttacccttga ccaacgctac gaggaaggcg
     2701 acgacgacga ggccattggg ctatcgcccg acaaggctgg tggtggagcc gccgacgatg
     2761 agctgctgaa catgctcaaa gagctccgca agaaaatggc caagaagcta aaccttccgc
     2821 cattcgtaat tttccaagac ccttcgctgg aggatatggc tattcaatac ccaattacaa
     2881 ttgatgaact gcagaacata tcgggtgttg gagcgggtaa agcgcgacgt ttcggtaaag
     2941 aatttgttga gcttattaag gcatacgttg aggaaaagga aattatccgt cctcaggata
     3001 tggttgttaa atcggtggca aacaaatcgc tcctgaaagt attcatcatc caaagcatcg
     3061 accgcaaaat ggattttgag gatattgctg aggctaagaa cctagagatg gatgaactgc
     3121 tcactgagat tgaggccatt gtaaattccg gaacacgcat caatatcgac tactacatca
     3181 acaaagtaat tgacgaggat aagcaggagg agatattcga ctacttccgt aacgaggctc
     3241 agaccgaaag tattgaggaa gcaatggctg cccttggcga tgatgatatc accgaggagg
     3301 aaatccgatt gatgcgtatc aagttcatgt cggaaatggg taactaaccc aattcccaag
     3361 tgggtatgca tacactactg tcaataaaca aactaaccaa gaactacgga acaaccgaag
     3421 cgctatgtaa cttcacgctc gaggttgttg aaggtcaggt actgggaatt cttggcccta
     3481 atggtagcgg taaaactaca ctactatcga tattgatggg cttacgatac ccaacctcag
     3541 gccactttac atggtttaac accccaccaa accatgttcc aaccaccagg attggggctc
     3601 ttattgaggt acccacattc tacccttacc tttcccttta cgataatatg cgtattagcg
     3661 cactggctaa gaatatcagc tataatgaga tttttcctgc cgttgagcgg gttggcctaa
     3721 cccccaacct aaaagcaaag gttgcgacct tctcccttgg aatgaagcaa cgcatggcta
     3781 ttgcacaagc cttgctgggt aacccaaaaa ttattgtact tgatgaaccc acaaacggct
     3841 tagaccccga ggggattgtt gaggttcgga acctaattaa agagctaaat gccggaggca
     3901 catccatcat agttgcaagc cacaacctcg atgaggttca acgtacctgt actcacgtgg
     3961 caatacttaa aaaaggtcac ctgcaagcct ttggtggggt caacgaactg ctcacatcgg
     4021 ccaaggtggc aattgttgag gttcgaaatc ctgaaaatct gattgatttc cttaacagca
     4081 accccactat tgaggttatt gaaagagtgg gtaattcatt caaattactg gtaaataaaa
     4141 gcgagcatca ggtaaccctt ataaccgatt ttcaaaatca cggaatagca gttaactctt
     4201 tcgaaatccg caacctaaac cttgaggagc tatttattac actagttcga aagtaaaaaa
     4261 acagcctatg atacgagcag taaaagcgga gctaattaaa acccttggtt atgcaacctt
     4321 ttgggtacta atttcgctcc atgccctcct tttcctgctg gttctaacaa ttgctaacaa
     4381 ggcaacactt aacttcaatg gagtagaatc caatatattg ctttcaaagc cctacacatg
     4441 gggtacagtt gcctggttag ccagctggtt taaccttatt ttagcccttt tattcattat
     4501 tctgtgcggt aacgattttc tgtttggcac ataccggcgc ggtttgctcg atggctttag
     4561 ccgcaataac ctgtttaagg caaagctatc gctcctaata ctaattgcct tttacgtggt
     4621 tgttctggta acgctaattg cgctttttgc aacccctgtg ctaagctctt gggaaaacct
     4681 tttaaccggc tacggttaca ttctggtact tttcattcaa atgcttggct acttttccat
     4741 agcgctc
//
LOCUS       DAOOWJ010000153         4717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_18179_length_4717_cov_10.316817, whole genome shotgun
            sequence.
ACCESSION   DAOOWJ010000153 DAOOWJ010000000
VERSION     DAOOWJ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18179_length_4717_cov_10.316817"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..439
                     /locus_tag="PLW22_11100"
     CDS             233..439
                     /locus_tag="PLW22_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75290.1"
                     /translation="MDIQTRKIHYTQEFLRVADDELVTKPEKELSPMTMKEFNAIIDK
                     SEDDFENERVTEAQKLLNQIDTWE"
     gene            682..1731
                     /locus_tag="PLW22_11105"
     CDS             682..1731
                     /locus_tag="PLW22_11105"
                     /inference="COORDINATES: protein motif:HMM:NF016903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HOU75291.1"
                     /translation="MGKPESNAKGIKGFFTHLITLIWWHLPIKREDRKRIKDFVFTRF
                     RPLFCRTKMYRNWKEADMLSGITFNEHDVDYPAHWPPVGHGKIRQPSPVAVDTSSIDA
                     LAIILHVYHVEILHEILRHLAKPSNQKVKLFVTYPPEKAEEINGVLSNSQADHQLLAY
                     ENRGRDVLPFLKTCAVAFEQGYGLILKLHTKKSDHRMAGSIWRKEIFNALLPVKARNE
                     IVEIFNGNPSVGILGPAGHIVPMSLYYGANAKGIGYLCHRLGVDTRELHGMAFVAGSM
                     FYARKEAILPLLELGMPDAVFEPEAGQLDGTMAHAYERGFAISSYAAGLWIVDNRFDP
                     RNPQPAITANHRFTW"
     gene            complement(2002..4023)
                     /locus_tag="PLW22_11110"
     CDS             complement(2002..4023)
                     /locus_tag="PLW22_11110"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU75292.1"
                     /translation="MSRQLRKRQAQKQQAVKTGRNYPFYIFTIFIVLLLPVIYHQATL
                     DLTTMPRIFALSIFLGLFILFFFYSKWFKPGMWGVLKYGIFPVFALWLVVSVVTLAFS
                     SNPIEGIFDIIKVLFIITIAILGAMIFSEEENWLDRLAKIVVLSAIITSLVGFVQYYL
                     WVVKATEPFLPDGRKIIYRVVGVMAHKNLYSLSLFMMLPFVAYGVVMFKKHWKILSTI
                     ALVFVLLLIFMLQARATWVGIMVAVAISVITLILYGNKFGLPKKWKITLITLTLMGAV
                     AVGGVLFLVGKDSQNQYVKQLQSIINPKSAQNIHRINIWKTTIEMIQDRPVLGYGPNN
                     WKLHAGYYFKGRFFLEDQINWQRPHNDFLWVFAEKGIFGILLYLAIFGFSFYYLYSVI
                     GAETTREKRLMALLLVFGLVGYLAASVFDFPYERIFHQAFLGIIFACSVALYHSVQPA
                     SVLKIKKHILVLPLVLVFVFGAYYGYRGTKQEVNLNKARAELRIINRGILPRIDSLPE
                     NQKKNAAAMLQQKWREVLKYALNSKTILKNLDPQANPIDYYIGLAYLNLKDYQNGLKY
                     CLLAHNQHPGSIKALNSLGAIYYNLEQYDMAEEYLQKSITIFPSHDALLNLSSTYFMQ
                     KEYDKAYELLSNAPEDIMSPRLENNLKSITIMREMAAKEAENSDTGDGK"
     gene            4281..>4717
                     /locus_tag="PLW22_11115"
     CDS             4281..>4717
                     /locus_tag="PLW22_11115"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HOU75293.1"
                     /translation="MKIAILGTRGVPNRYGGFEQFAQYLSLGLAWLGADVWVYNSHDH
                     PHKEKKWQGVNIIHRFDPERYIGASGQFIYDLNCILHSRRRDFDIILQLGYTTSTIWH
                     WLLPRKSLIVTNMDGLEWKRSKYGKLLQRFLRHAERWAVKSSHR"
BASE COUNT         1370 a         1066 c         1033 g         1248 t
ORIGIN      
        1 gagttcaaat cgcttaccat gactaatgat ttaatgggtt aaactatcag attattatta
       61 attttgacga ttaacttcga aaaaacgtca tttctctgct acgaagttat ggaatgcaat
      121 tgaaaagggc aaattattta atacctataa ttaagggcaa gggtcataaa atcggaacac
      181 tttacttaaa atgaattaat ccttatcttt agagaaagaa aaatttaaca aaatggacat
      241 tcaaacaaga aagatacact acacacaaga gttccttaga gttgctgacg atgaacttgt
      301 caccaaacct gaaaaagagt tgagtccaat gacaatgaaa gaattcaatg caatcattga
      361 taaatcggag gacgattttg aaaatgaaag agttacagaa gcacaaaaac ttctcaacca
      421 aattgataca tgggagtaaa ggttctgtgg catgatacat ctcttgttca accccaattg
      481 ctagaatcgt ttttgtattt gattgcaggc agaatcccaa aaagttagtc ctaataaagg
      541 aataaccccc tgcccctaac actattattt tttcaaatta tctctttggc gaaagccatc
      601 aagaatattc gcgcgaaaaa ggtcatattg attttcaatt cctatttttg taaagctatt
      661 gatttggcat aaacagcaaa aatggggaaa ccggaaagca atgcgaaagg gatcaaaggt
      721 tttttcactc acctgattac cctgatatgg tggcacttgc caataaaacg cgaggacaga
      781 aaacgcatca aagattttgt gttcacccgc ttccgccccc tattttgccg tacaaagatg
      841 taccgcaact ggaaagaggc ggatatgctc tcggggataa cattcaacga acacgatgtt
      901 gactaccccg cccattggcc gcccgttggc cacggcaaaa ttaggcaacc atccccggtg
      961 gctgttgata catcgtccat tgatgcccta gccattattc tgcacgtgta ccacgtggaa
     1021 atcctacacg agatactccg ccatttggcc aagccatcaa accaaaaggt caaactgttc
     1081 gttacctacc ctcccgaaaa agcagaggaa atcaacgggg tgctttcaaa ttctcaagcc
     1141 gaccatcagc ttttggccta tgaaaatcgc gggcgcgatg tgctgccatt cctcaagacc
     1201 tgtgccgttg cctttgaaca gggttatggg ttaatcctga aactccacac caaaaaatcg
     1261 gatcaccgca tggcgggaag tatttggcgc aaggagattt tcaacgcatt acttcctgta
     1321 aaagcgcgca acgaaatcgt tgaaattttc aatggcaacc cctccgtcgg tatccttggc
     1381 ccggcggggc atattgtacc catgagcctg tactacggtg ccaacgcaaa gggcattggc
     1441 tacctatgcc acaggcttgg ggttgatacc cgtgagctgc acggtatggc tttcgtagcc
     1501 ggttccatgt tctatgcaag gaaagaggcc attttgcccc ttttggaact tggtatgccc
     1561 gatgcagttt ttgaacccga ggcgggtcag ctggatggca ccatggccca cgcctacgaa
     1621 cgcgggtttg ccatcagcag ctatgctgcc ggactgtgga ttgtggacaa tcggtttgac
     1681 cctcggaatc cccaacctgc cattaccgcc aaccataggt ttacgtggta gaaaaagagg
     1741 cgaggttcga ggttgtttaa gcatgcggtg gagagacagc tttgtcgctt cgctcctcgc
     1801 agtgacgggg gggcgtgaaa cgtaactcgt accccgcacc ttgcaatccg cttaaccgca
     1861 gaagtcacag agcaggcgca aagaccacag agttactttt gcatctcgca acctgccatt
     1921 accgccaacc ataggtttgc gtggtgaaca tggggtgttt aatttatgaa ttgattcaca
     1981 aagatctact gtacagaaag cctacttccc atcccccgta tcggagtttt cagcctcctt
     2041 ggcagccatt tccctcatta tggtaatgga ctttaaatta ttttccagcc ttggcgacat
     2101 gatatcctcc ggagcattgg aaagcagttc gtaagcctta tcgtactctt tctgcatgaa
     2161 atacgtggac gacaggttaa gaagcgcatc gtgcgaggga aagatggtaa tggatttttg
     2221 cagatattcc tctgccatat cgtactgctc cagattgtag tatatcgcac ccaaactatt
     2281 caatgcctta atgctgccgg gatgttgatt gtgtgccaaa aggcaatact taagcccgtt
     2341 ctgataatcc ttcaaattaa ggtaagccaa tcctatgtaa taatctatcg ggtttgcctg
     2401 cggatcgagg ttttttaaaa tggtcttgga attcaaggca tacttcaaca cttcgcgcca
     2461 tttttgttgc agcatggctg cagcattttt cttctgattt tcgggcaacg aatcaattcg
     2521 cgggagaata cctctattaa ttatcctcag ttcggccctt gccttgttca gattaacctc
     2581 ctgctttgta ccacgatacc cgtagtatgc cccaaatacg aagacaagaa caaggggaag
     2641 caccagaata tgctttttga tttttaatac cgatgcgggt tggacgctat ggtatagcgc
     2701 taccgaacag gcaaatatta tccctaagaa agcctgatgg aaaatcctct cgtatgggaa
     2761 gtcgaatacc gatgcagcaa ggtagcccac caatccgaag acaagcaaaa gggccatgag
     2821 cctcttttcg cgtgtagttt ctgcaccaat aacgctatac aggtagtaaa atgaaaatcc
     2881 aaaaatggcg aggtagagca aaatcccaaa gattcccttt tcggcaaaga cccatagaaa
     2941 atcgttgtgg ggtcgttgcc agttaatctg atcctccagg aagaatcgac ctttgaaata
     3001 ataacccgcg tgcaacttcc aattgttggg tccataaccc aataccggcc tatcctgaat
     3061 catttctatg gttgttttcc aaatatttat cctgtggata ttttgggccg acttgggatt
     3121 aataatggat tgcaactgct taacatactg attctggcta tctttcccaa ctaaaaagag
     3181 aacccctcca actgctactg cacccatgag ggtgagggta atcagggtga ttttccattt
     3241 tttgggcaag ccaaatttgt taccgtaaag gataagcgtt atcactgata tggctactgc
     3301 aaccataatc cccacccagg ttgccctggc ctgaagcata aagattagca acaacacaaa
     3361 aactaaggca atggttgaca aaattttcca atgtttctta aacattacca ctccatacgc
     3421 tacaaagggg agcatcatga atagcgaaag cgagtatagg tttttatggg ccatcacccc
     3481 aaccaccctg tatataattt ttcgcccatc gggcaaaaaa ggctcagttg ccttgaccac
     3541 ccaaaggtaa tactgcacaa agcccaccag cgaggtgata atggccgaaa ggacaactat
     3601 cttagccagc ctatcaagcc aattctcctc ttcggaaaaa atcatggccc ccagtatggc
     3661 aatggtaatg ataaacaata ccttaataat atcgaaaatg ccctcgatgg gattagagga
     3721 gaatgccagc gtaaccaccg ataccacaag ccaaagcgca aaaacaggga aaataccata
     3781 ttttagtact ccccacattc cgggtttaaa ccatttggag taaaagaaaa acagtatgaa
     3841 caaaccgagg aatatgctca gggcaaatat tctgggcatc gtggtcaggt caagggtggc
     3901 ctgatggtag ataacgggca gtaatagcac aataaaaatg gtgaagatgt agaatgggta
     3961 gttgcgaccg gttttaacag cctgctgctt ttgcgcttgt ctctttcgta attgcctgct
     4021 catgattggg ttagttagtg ttttgccaat tagtaatggt aacttttctc caagggtttt
     4081 tgttggctac taaaattatt aatggctcaa aaataaacat tttgcttcat attgactgcc
     4141 atcggacgtg tattaaattt gcgaaccatg ttgatttgca ataatgataa gactactcat
     4201 ggccgtttgt tgcattttta ttctattttt gcttatccat aaatgtcccg tgggcactga
     4261 tataatagtt tgagttaagc atgaagatag ccattcttgg aacacgtgga gtaccaaacc
     4321 ggtatggagg gttcgagcaa tttgcccagt acctgtcgtt agggttagcg tggctgggtg
     4381 ccgatgtttg ggtttacaat tctcacgatc atccacataa ggagaaaaaa tggcaggggg
     4441 tgaacatcat tcaccgcttc gaccccgagc gatatattgg tgccagtggt cagtttatct
     4501 acgacctaaa ctgtattctc catagccgca ggcgcgattt cgatatcatt ctccaattgg
     4561 gctacacaac cagcaccatc tggcattggc tattgccccg caaatcgctg atagttacca
     4621 acatggacgg gctggagtgg aagcggagca agtacggcaa gttgcttcaa cgttttttaa
     4681 ggcatgccga aagatgggca gtgaaaagca gccatcg
//
LOCUS       DAOOWJ010000154         4620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_18676_length_4620_cov_5.372837, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000154 DAOOWJ010000000
VERSION     DAOOWJ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18676_length_4620_cov_5.372837"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLW22_11120"
     CDS             <1..214
                     /locus_tag="PLW22_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOU75294.1"
                     /translation="LKFDAEVLDYIVDKAVEFKLGARGLRSICEAIMIDAMFELPTSE
                     QKEMVVTLEYAQNKLEKSNLKRLKAA"
     gene            complement(211..696)
                     /locus_tag="PLW22_11125"
     CDS             complement(211..696)
                     /locus_tag="PLW22_11125"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOU75295.1"
                     /translation="MKISIIVAIAENGVIGGGNELLWHIPDDLKRFKRITSGHSVIMG
                     RKTFESIGKPLPKRRNIVITRNSEFRVDGIEVANSLRTALEMTKSEDEVFVVGGGEVY
                     RQALPLANKLYITRVHDKYEGDTFFPDIDMNDWQPVSSEKGNPTTGPGYTFEEYVRIK
                     H"
     gene            complement(711..1505)
                     /locus_tag="PLW22_11130"
     CDS             complement(711..1505)
                     /locus_tag="PLW22_11130"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOU75296.1"
                     /translation="MVQYLNLLDHILNHGVRKDDRTGTGTISVFGYQMRFDLQEGFPL
                     LTTKKLHLKSIIHELLWFLKGETNIRYLNENGVTIWDEWADANGELGPVYGYQWRSWP
                     TADGRHIDQISRVVDSLKNNPDSRRHIVSAWNVGEIENMALPPCHVLFQFYVANGKLS
                     CQLYQRSADVFLGVPFNIASYALLTMMMAQVCGYEPGEFVHTLGDTHIYLNHIEQVKL
                     QLTREPRKLPRMVINPEVKSIFDFKFADFKLEDYNPHPHIKGDIAV"
     gene            complement(1568..2548)
                     /locus_tag="PLW22_11135"
     CDS             complement(1568..2548)
                     /locus_tag="PLW22_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOU75297.1"
                     /translation="MRSKLLAIAVSLRFLIGSFSERILGCLKRAFPKALKTSFWILKI
                     TLPISLLVSVLSYFGLLNAISVFLTPAFKVIGLRGEAAIAFVTGVFLNIYSVIAVLSQ
                     LTLTLREVTIIAVMSLIAHNLIVETTVQSKTGSKAWHMVALRLGMAFLAAFMLNLTLP
                     PLDQAFGHPANHEQTLSLVAHLQGWLFSAFRMIFKIIVLIFLLMVLQEVLREFNLIRV
                     LTAPMSPIIKLLGLPQSTSFLWIIANTLGLAYGSAVMLDEVSQGRIRKAESDLLNYHI
                     AISHSNLEDLLLFAAIGVSIPWMLGIRLIIAALAVWARRWYLSAKGEVFI"
     gene            complement(2627..2708)
                     /locus_tag="PLW22_11140"
     tRNA            complement(2627..2708)
                     /locus_tag="PLW22_11140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2672..2674),aa:Leu,seq:tag)
     gene            complement(2774..3499)
                     /gene="lptB"
                     /locus_tag="PLW22_11145"
     CDS             complement(2774..3499)
                     /gene="lptB"
                     /locus_tag="PLW22_11145"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOU75298.1"
                     /translation="MRLYTDTLVKRYKTRTVVKGASVEVSQGEIVGLLGPNGAGKTTT
                     FYMIVGLIKPNSGRIFLDDVEITDEPVFRRAQRGIGYLAQEASVFRQLSVEDNIKAVL
                     EMTNFSKEYQHEKLESLLNEFGLQKIRKSLGIQLSGGERRRTEIARALALDPKFILLD
                     EPFAGVDPIAVEDIQNIVAHLKDRNIGILITDHNVHETLSITDRAYLLYDGEILKAGT
                     AEELADDEQVRKLYLGQNFELRK"
     gene            complement(3511..3777)
                     /locus_tag="PLW22_11150"
     CDS             complement(3511..3777)
                     /locus_tag="PLW22_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75299.1"
                     /translation="MSALINNNRGIVVFNVVKNSDGSENSKRIRNATTNDTAQIKISI
                     RKIIHRGVFFGGIIFFFKVKNYLRFKKQEMKNICKNSYLYAQIE"
     gene            3755..>4620
                     /locus_tag="PLW22_11155"
     CDS             3755..>4620
                     /locus_tag="PLW22_11155"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOU75300.1"
                     /translation="MLFIRALTFVAFRTYAGLIRYTGTRDAENIFIVNSLGTFILFII
                     NFINHVNTNTFLIPNSILLIDFLITVLLLIGSRLTYKAIYYTIKNIDFQGDNVLIIGI
                     EQFAAAVKKAIDRESLSGIRIVGFIDPYNQQGGNKIENVGIFNTNQLDFLVQKFDVKK
                     VILATKNLDPNRKNKIIEKCLNLNVSVQTIPDANAWINGELNVKQIKNIKIEDLLERP
                     PIVLDKKRIQDYIKGKVVLVTGAAGSIGSEMVRQITRFRPNKIILFDVAETPLYELEL
                     ELKETFQFFDFET"
BASE COUNT         1392 a          990 c          927 g         1311 t
ORIGIN      
        1 cctgaaattc gatgccgagg ttcttgatta cattgtagat aaggcagtag agtttaaact
       61 gggtgctcgc ggcctacgct caatttgcga agccattatg attgatgcaa tgtttgaact
      121 cccaaccagc gaacagaagg agatggttgt tacgctggaa tatgctcaga ataagctgga
      181 aaaatcaaat cttaaaaggt taaaagctgc ttaatgctta atcctaacat actcctcaaa
      241 ggtgtaaccc ggccccgtgg tcgggtttcc tttttctgag ctaaccggtt gccaatcatt
      301 catgtcgata tcggggaaaa atgtatcgcc ctcgtattta tcgtgcacac gggtgatgta
      361 aagtttgttg gccaagggca aggcttggcg gtaaacctcg ccaccaccta ctacaaaaac
      421 ttcgtcctcg ctttttgtca tttccagtgc ggttcgtagc gagtttgcca cctcaatccc
      481 atcaaccctg aattcagagt ttcgagtaat cactatattt cgtcgtttgg gaagcggttt
      541 accaatgctt tcgaaggttt tacgtcccat aataacactg tgccccgatg tgattctctt
      601 gaaccgtttc aggtcatcgg ggatgtgcca gagtaactca ttccctccgc caataacacc
      661 gttctcagca attgcaacaa taattgagat tttcataggc ctgtccataa ctacacagca
      721 atatcaccct taatgtgtgg gtgcgggtta taatcctcca gcttgaaatc agcaaactta
      781 aaatcaaaaa tcgatttaac ctcagggttt attaccatac gtggtaactt tcggggctcg
      841 cgggtcagct gtagcttaac ctgctcaatg tggtttaggt agatatgcgt atcgccaagg
      901 gtgtgaacaa actcaccagg ttcatacccg caaacctggg ccatcatcat ggttaacagg
      961 gcataggaag ctatattaaa aggaacgcca aggaacacat cggcgctgcg ctggtaaagc
     1021 tgacacgaaa gcttgccgtt ggcaacatag aactggaata gaacatgaca gggcggtaag
     1081 gccatattct caatttcacc tacattccat gccgatacga tatggcgccg tgaatcgggg
     1141 ttatttttga gggaatcaac caccctactt atctggtcaa tatgccttcc atcggctgtt
     1201 ggccaactac gccactggta gccataaacc gggcccaact cgccgttggc atcggcccac
     1261 tcatcccata tggttactcc attctcgttc aggtaccgga tgttggtttc ccctttgagg
     1321 aaccataaaa gctcatggat tatcgatttc aggtgaagct ttttggtggt aagcaacgga
     1381 aagccctcct gcaaatcgaa gcgcatctgg taaccaaaaa cactaatggt tcctgttcca
     1441 gttcgatcgt ctttacgtac accatgatta aggatgtgat cgagcagatt aaggtattga
     1501 accatgacca aacatttttc tgctcaaaaa tataaaagaa aaacgaagcg agcgtgagct
     1561 gttgatatca aatgaaaact tctccttttg cagatagata ccacctgcga gcccacactg
     1621 ccagggcagc aataatcagt ctaatgccta gcatccatgg aattgataca ccaatggctg
     1681 caaacaggag caaatcctcc aggtttgagt gcgaaatggc aatatggtag ttaagcaaat
     1741 cggattcggc ctttctaatt cttccctggc taacctcatc gagcattaca gccgaaccgt
     1801 aggcaagccc taacgtgttt gcaataatcc acaggaacga tgttgattga ggcaggccaa
     1861 gtaattttat tatgggcgac attggagctg ttaaaaccct tatgagattg aactccctga
     1921 gaacttcctg caataccatt aacaggaaaa taagaacgat aatcttaaaa atcattctaa
     1981 atgcagagaa cagccatccc tgcaggtgag caacaaggct tagggtttgt tcatgattgg
     2041 cagggtgtcc aaaagcctga tctaaagggg gaagagttag gtttagcata aaggcagcta
     2101 aaaaagccat tcctaatcgt aaagcaacca tatgccatgc tttagagccg gttttcgact
     2161 gcacagtggt ttcaacaata agattgtgag ctatgaggct cataacggca ataatggtaa
     2221 cctcacgtag tgttaaggtt aactgcgata ataccgctat tactgagtag atattcagga
     2281 aaactccagt tacaaaggct atggccgcct cgccccgtaa accaatcacc ttgaatgccg
     2341 gggttaaaaa aacgctaatt gcatttagca atccaaagta actcaaaaca cttaccaata
     2401 acgatatggg taatgtaatc ttaagtatcc aaaacgaagt ttttagtgct tttggaaaag
     2461 ctctcttgag acatcccaaa attctttcgg aaaaagaacc aattaaaaac cttaagctga
     2521 ctgcaatggc caaaagtttg cttcgcatat caataactat tgttgctatg atgcagaacc
     2581 tatgtaaagg tttaaaaaag aataagcccg gttatcccgg gcttagtgcg gatagaggga
     2641 ctcgaacccc cacgcctttc ggcactagat cctaagtcta gcgcggctac caattacgcc
     2701 atatccgcaa aatttcggtg caaagttata aagtattttt aaaacaccaa taacctcact
     2761 ttattttgga taattatttc ctaagctcaa agttttgtcc caggtatagc ttacgaacct
     2821 gttcatcatc ggcaagttct tcggcagtac cagctttaag tatttcgcca tcgtaaagca
     2881 ggtaagccct atcggtaatg gaaagtgttt catggacatt atggtcagtg ataagaatac
     2941 cgatgttcct atccttaagg tgggcaacaa tgttctgaat atcctccacg gcaatgggat
     3001 caaccccggc aaagggctca tcgagcagga tgaacttggg gtcaagggca agcgctcggg
     3061 ctatttcggt tctacgtcgc tcaccgcctg aaagctgtat acctaaactt ttccgaattt
     3121 tttgtaggcc aaactcattg agcaacgact ccaacttctc gtgctggtat tccttactga
     3181 aattagtcat ctccagtacg gctttaatgt tatcctcaac gcttagctgc ctaaaaacag
     3241 aagcttcctg tgcaagatag ccaataccgc gttgtgcacg acggaacacc ggttcgtcgg
     3301 ttatctcaac atcatcaagg aaaatcctgc cggagttcgg cttaatgaga ccaacaatca
     3361 tatagaaagt tgtggtttta cctgccccat tagggccaag caaccctaca atctcccctt
     3421 gacttacctc aacgcttgca cctttaacaa cagtacgagt cttgtatcgc ttaaccagtg
     3481 tatcagtata gagccgcata gtacagttag ctattcaatt tgtgcgtaaa ggtaactatt
     3541 tttgcaaata tttttcattt cctgtttttt gaatcttaag taatttttaa ctttgaaaaa
     3601 aaatattatg cctccaaaaa atactccacg atggataatt ttcctgattg atatcttaat
     3661 ttgtgcagta tcatttgtag ttgcattcct aatccgtttt gaattttcgc ttccatctga
     3721 attcttcaca acattaaaaa ctacaattcc tctattgtta tttattaggg cactcacttt
     3781 tgttgcattt agaacatatg cgggattaat ccgttataca ggtacaaggg atgccgagaa
     3841 tatttttatt gtaaatagtc ttggtacttt tatcctattt attataaatt ttataaatca
     3901 cgtcaataca aacacatttt taattccaaa ctccatactt ttaattgact ttttaataac
     3961 tgttttgcta ctaataggtt cgcgcttaac atataaagcc atttattata caataaaaaa
     4021 cattgatttt caaggtgata atgtattaat cataggtatt gagcagtttg ctgctgctgt
     4081 aaaaaaagca attgataggg aaagtttatc gggtataaga attgtcggtt ttatagatcc
     4141 atacaatcaa cagggaggta acaaaattga aaatgttgga attttcaaca ctaatcaatt
     4201 agattttctg gttcaaaaat ttgatgttaa aaaggttatt cttgcaacca agaatcttga
     4261 tccaaaccga aaaaacaaga ttattgaaaa gtgtttaaac ctaaacgtta gcgttcaaac
     4321 catccccgat gcaaatgcat ggattaacgg ggaactcaat gtaaagcaga ttaaaaatat
     4381 taaaattgag gatttacttg aaaggccacc cattgttctt gacaaaaagc gaatacagga
     4441 ctacattaag ggtaaagtgg tcttggtaac aggtgctgcc ggatcaattg gtagtgagat
     4501 ggtacggcaa atcaccaggt ttcgtcccaa taagatcatt cttttcgatg tggccgaaac
     4561 acccctatac gaattggagc ttgagctgaa ggaaactttt cagtttttcg actttgaaac
//
LOCUS       DAOOWJ010000155         4600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_18797_length_4600_cov_8.975138, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000155 DAOOWJ010000000
VERSION     DAOOWJ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18797_length_4600_cov_8.975138"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLW22_11160"
     CDS             complement(<1..437)
                     /locus_tag="PLW22_11160"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HOU75301.1"
                     /translation="MKIAILGTRGVPNRYGGFEQFAQYLSLGLMQLGAEVWVYNSHDH
                     PLKEKKWQGVNIIHRFDPEQYIGASGQFIYDLNCILNSRRCDFDIILQLGYTTSTLWH
                     WLLPRKSLIVTNMDGLEWKRSKYGKLLQRFLRHAERWAVKSSHR"
     gene            680..2701
                     /locus_tag="PLW22_11165"
     CDS             680..2701
                     /locus_tag="PLW22_11165"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU75302.1"
                     /translation="MSRQLRKREAQKQQEVKTGRNYPYFIFTIFIVLLLPVIYYQATL
                     DLTTMPRVLALSAFLGMFVIFFFNSKWYKPEMLGVLRHGLFPVFGLWLVVSVVTLAFS
                     SNPIEGIFDIIKVLFTITIAMLGAMIFSEEENWLDKLAKVVVLSAIVTSLVGFVQYYL
                     WVVKATEPFLPDGRKIIYRVVGVMAHKNLYSLSLFMMLPFVAYGVVMFKKHWKILSTI
                     ALVFVLLLIFMLQARATWVGIMVAVAISVITLILYGNKFGLPKKWKITLITLTLMGAV
                     AVGGVLFLVGKDSQNQYVKQLQSIINPKSAQNIHRINIWKTTIEMIQDRPVLGYGPNN
                     WKLHAGYYFKGRFFLEDQINWQRPHNDFLWVFAEKGIFGILLYLAIFGFSLYYLYRII
                     GAETTREKRLMALLLIFGLVGYLTASTFDFPYERIFHQAFLGVIFACSVALYHSVYPT
                     AVLKIKKDILVIPLVVIFVFGAYYGYRGTKQEVNLNKARAELRIINRGILPRIDSLPE
                     NQKKNAAAMLQQKWREVLKYALNSKTILKNLDPQANPIDYYIGLAYLNLKDYQNGLKY
                     CLLAHNQHPGSIKALNSLGAIYYNLEQYDMAEEYLQKSITIFPSHDALLNLSSTYFMQ
                     KEYDKAYELLSNAPKDIMSPRLEDNLKSITIMREMAAKEAKNAEKANGK"
     gene            complement(3275..4324)
                     /locus_tag="PLW22_11170"
     CDS             complement(3275..4324)
                     /locus_tag="PLW22_11170"
                     /inference="COORDINATES: protein motif:HMM:NF016903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HOU75303.1"
                     /translation="MGKPENNAKGVKGFFTHLITLLWWHLPIKREGRKRIKDFVFTRF
                     RPLFCHTKVYRNWKEADMLSGITYNENDVDYPAHWPSVGHGKIRQPSPVAVDTSPIDD
                     LAIILHVYHVEIMHEILRYLVKPSNQKVKLFVTCPHEKAEEINGVLSNSHIYHELLAF
                     ENRGRDVLPFLKACAVAFEQGYGLILKLHTKKSDHRMAGSIWRKEIFNALLPAKARTD
                     IIEIFNGNPSVGILGPTGHIVPMSLYYGANAKGIGYLCRRLGVDTRELHDMAFVAGSM
                     FYARKEAILPLLELDMPDAVFEPEAGQLDGTMAHAYERGFAISSYAAGLWIVDNRFDP
                     RNPQPAITTNHRFTW"
BASE COUNT         1270 a          947 c         1044 g         1339 t
ORIGIN      
        1 cgatggctgc ttttcactgc ccatctttcg gcatgcctta aaaaacgttg aagcagtttg
       61 ccatacttgc tccgcttcca ctccagccca tccatattgg taactatcag tgatttgcgg
      121 ggtagcagcc aatgccagag ggtgctggtt gtgtaaccca attggagaat gatatcgaaa
      181 tcgcacctgc ggctattgag aatacagttg aggtcgtaga taaactgacc actggcacca
      241 atatattgct cggggtcgaa gcggtgaatg atgttcaccc cttgccattt tttctcctta
      301 agtggatgat cgtgagaatt gtaaacccaa acctcggcgc ccagttgcat taaccccagt
      361 gacaggtact gggcaaattg ctcaaatccc ccatacctgt tgggtactcc acgcgttcca
      421 agaatggcta tcttcatgct tatccaaagt aattacttca atgccctatt gcagtttgcc
      481 gataggcaaa aatagaacaa aaatggaata aacagccatt cgctaatcct cattacgcaa
      541 tggctgcaaa atttataaat ggccaaaggc agtcaatgtg aagcaaaata cttacttttg
      601 agccaatgat gattcaatag ttccgttgga attgtccgat taaaataatg ttgttcaaaa
      661 cgaaataact cactaaatca tgagcaggca gctacgaaag cgtgaagcgc aaaaacagca
      721 ggaagtgaaa acggggcgta attaccccta cttcattttt accatattca ttgtattgct
      781 acttcctgtt atttactatc aggcaaccct cgatttgacc acaatgccca gagtattggc
      841 attgagcgca ttcctcggaa tgtttgtcat tttctttttt aattcgaagt ggtataagcc
      901 cgaaatgctt ggcgtattga ggcatggcct atttcctgta tttgggctct ggctcgtggt
      961 gtcggtggtt acgctggcat tttcctccaa tcccatcgag ggcattttcg atattattaa
     1021 ggtattgttt accattacca ttgccatgct gggtgccatg attttttccg aggaggagaa
     1081 ttggcttgat aagctggcta aggtggttgt tctttcggcc attgtcacct cgctggtggg
     1141 ctttgtgcag tattaccttt gggtggtcaa ggcaactgag ccttttttgc ccgatgggcg
     1201 aaaaattata tacagggtgg ttggggtgat ggcccataaa aacctatact cgctttcgct
     1261 attcatgatg ctcccctttg tagcgtatgg agtggtaatg tttaagaaac attggaaaat
     1321 tttgtcaacc attgccttag tttttgtgtt gttgctaatc tttatgcttc aggccagggc
     1381 aacctgggtg gggattatgg ttgcagtagc catatcagtg ataacgctta tcctttacgg
     1441 taacaaattt ggcttgccca aaaaatggaa aatcaccctg attaccctca ccctcatggg
     1501 tgcagtagca gttggagggg ttctcttttt agttgggaaa gatagccaga atcagtatgt
     1561 taagcagttg caatccatta ttaatcccaa gtcggcccaa aatatccaca ggataaatat
     1621 ttggaaaaca accatagaaa tgattcagga taggccggta ttgggttatg gacccaacaa
     1681 ttggaagttg cacgcgggtt attatttcaa aggtcgattc ttcctggagg atcagattaa
     1741 ctggcaacga ccccacaacg attttctatg ggtctttgcc gaaaagggaa tcttcgggat
     1801 tttgctctac ctcgccattt tcggattttc attgtattac ctgtatagga ttattggtgc
     1861 cgaaaccacc cgcgaaaaaa ggctgatggc ccttttactt atcttcggat tggtgggtta
     1921 cctcacagca tcaacttttg acttccccta tgaaaggata ttccaccagg cattcttagg
     1981 ggtaatattt gcctgttcgg tagcactcta ccatagtgtt tatcccacag ctgtattaaa
     2041 aatcaaaaag gatattctgg taattcccct tgttgttatc ttcgtttttg gggcatacta
     2101 cgggtatcga ggtacaaagc aggaggttaa tctgaataag gcaagggccg aactgaggat
     2161 aattaataga ggtattctcc cgcgaattga ttcgttgccc gaaaatcaga agaaaaatgc
     2221 tgcagccatg ctgcaacaaa aatggcgcga agtgttgaag tatgccttga attccaagac
     2281 cattttaaaa aacctcgatc cgcaggcaaa cccgatagat tattacatag gattggctta
     2341 ccttaatttg aaggattatc agaacgggct taagtattgc cttttggcac acaatcaaca
     2401 tcccggcagc attaaggcat tgaatagttt gggtgcgata tactacaatc tggagcagta
     2461 cgatatggca gaggaatatc tgcaaaaatc cattaccatc tttccctcgc acgatgcgct
     2521 tctcaacctg tcgtccacgt attttatgca gaaagagtat gataaggcat atgaactgct
     2581 ctcaaatgct cccaaggata tcatgtcacc aagactggaa gataatttga agtccattac
     2641 cataatgcgg gaaatggctg ccaaggaggc taaaaatgca gaaaaggcga atgggaagta
     2701 ggctgtttgc ttgtctttgt agtgtagtta attgggatgt aagtgtccaa gtggtatgcc
     2761 aacaatagat attcacgacc cttttatatt gatgttcagt atcagtttgt tgccggagag
     2821 tagttaggag ttgattgttc tatgtataca tatttatggg gctaaagccc ttttgtgtac
     2881 tacaaatttt gcccgtcagc taaagctgac ggcaattgaa gaaatagaat tattgctttg
     2941 tgaatattgt tggtactgaa gtctcagaat atgtatttcc caatccgggc ttaagcccgg
     3001 atgcaatatc aaatacttta agacaatttt taattaattt tcattgccgt cggcttaagc
     3061 cgacgggata cttaaactaa aattcattag gctttagcct cattattggt tgtataggct
     3121 ttgaaggaga aaaattaact gggaaatcaa aagtatgatt gacaggtatc tatgatccga
     3181 cgaattatta aatgaacttc aaagaaaagt ggagaaggtt cacccgataa aaacggtttt
     3241 tttgtgaaac atttggcgca aaacactccc atattcacca ggtaaaccta tggttggtgg
     3301 taatggcagg ttggggattc cggggatcaa accgattgtc cacaatccat agtccggcag
     3361 catagctact gatggcaaaa ccgcgttcgt aggcgtgtgc catggtacca tccagttgac
     3421 ccgcttcggg ttcgaaaact gcatcgggca tatcgagttc cagaaggggc aggatggcct
     3481 ctttccttgc atagaacatg gaaccggcaa cgaaagccat gtcgtgcagc tcgcgggtat
     3541 caacgccaag cctacggcat aggtaaccaa tgccctttgc gttggcaccg taatacaggc
     3601 tcatgggtac aatatgcccc gttgggccaa ggatgccgac ggagggattg ccattgaaaa
     3661 tttctatgat gtcggtgcgc gcttttgcag gtaacaatgc gttgaaaatc tctttacgcc
     3721 aaatactccc cgccatgcgg tgatccgatt ttttggtatg gagtttcaga atcaaaccat
     3781 aaccctgttc aaaggcaacg gcgcaggcct tgaggaatgg cagcacatcg cgcccccgat
     3841 tttcaaaagc caaaagttca tggtagatat gagaatttga aagcacaccg tttatttcct
     3901 ctgctttttc gtgagggcag gtaacgaaca gtttgacctt ttggtttgat ggcttgacca
     3961 gatagcggag tatctcgtgc atgatttcca cgtggtacac gtgcagaata atggccaggt
     4021 catcaatggg cgatgtatca acggccaccg gggagggttg cctgattttg ccatggccaa
     4081 cggatggcca atgggcggga tagtccacat cgttttcgtt gtatgttatc cccgagagca
     4141 tatccgcctc tttccagttg cggtacacct ttgtatggca aaacaggggg cggaagcggg
     4201 tgaacacaaa atccttaatg cgttttctgc cttcgcgctt gattggcaag tgccaccata
     4261 acagggtaat caggtgagta aaaaaacctt tgaccccttt cgcattgttt tccggtttcc
     4321 ccattttcac tgtttatggc aaatcaatag ctttacaaaa ataggaattg aaaatcaata
     4381 tggccttttt cgggagaata ttcttgatgg cttaacccga tgagataatt tagaaattta
     4441 atagtattaa ttataggtat taaaatattt gccctttcca atttcatttc ataacttcgt
     4501 agcacagaaa tgacgttttt tcgaagttaa ttgataaaat taattataat ctgatagttt
     4561 aacccattaa atcattagtc atggtaagcg atttgaactc
//
LOCUS       DAOOWJ010000156         4488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_19445_length_4488_cov_7.663208, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000156 DAOOWJ010000000
VERSION     DAOOWJ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19445_length_4488_cov_7.663208"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PLW22_11175"
     CDS             <1..1296
                     /locus_tag="PLW22_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75304.1"
                     /translation="ISVLGLNPKQQKYIDRVLMSKFSIVTYDRFKVNYYRLMSDKVFA
                     RIQPQFVYDRNSGLFDVKMQAILNRHPSLGLGLSLSSDNGNEGFFSITHNWLTRTSNT
                     LYGNIYFGKFHSSARLTAMKTFPSRIPVSLVSTLVANRYDYHSGNPVPFFEDVKPAYA
                     IQNETLVTIALRLSHTSALNTSMFVTSGEKMDEYYQTENYYSFDIPDKTRFRFFKGGI
                     RLEKNTLNHKQYANRGRHQLVSISGYYGHEIHKPGTTAMQLSEFDNYQSFITAYLHNE
                     SYHRLIGKSFWLGFYLDGYWSSQRFFDNYWATILSMNQFSPTPHSRIMFLKELRSSKY
                     VAIGLSPVVDFGSNVSFRIDAFVYQPYKAILANSDGLAYYGDAFKSREYLMSASVIYS
                     SPVGPVTITVSHYPRNKGKQFFLNLSFGYSLFNPRVFDN"
     gene            complement(1293..3083)
                     /locus_tag="PLW22_11180"
     CDS             complement(1293..3083)
                     /locus_tag="PLW22_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU75305.1"
                     /translation="MKIANKLIEVLSFGVDTNKSKDFADKITQLNLIAIPSLLIFIIL
                     AITSLKASLPVAIINFSTFIVALVLLFMLFRSSRFSSIGLGISYIFAIGILVNILLGS
                     NNLLLLAVTMLLPLMFFSSTPFNKASVTLVAFAVLLPIVLFILPVIGLPAENIFYVNR
                     YFISIGFIVYLTSVSLLWFIRTKQLQELEIKSIELKNDIKKRTENLAKLSHDIRTPLN
                     NIVIISNILSNQIQEEKLKDMIDTIQASSNNLMSTLNSMIDLSISEMPKDSDVLVPFD
                     LQNTLNNTFRLFSTQSGTVSFSFKYDERLPKNLLGDPVKLKQIFLNIVESIIKNKSSQ
                     KVILDIKINKMQEAGDGVDIYTEIKTNTPLLLHHADGVSSTTLTDGSTALSNQVFIEM
                     LDLKIAAKLIRENGGKLNVKLTSQDSQINFNIRLLKVPTALKEEIQQAEGKEKGINEI
                     VPETPEVSKVEKLPTTDVRLENANVLLVEDNLINQKIVLLSLKKLVKNIDVAVNGKEA
                     LDKFGTSKYDIILMDVQMPIMNGYTAAKKIREIEASTNSHTPIIAITANALMGDREEC
                     LAAGMDDYISKPFQIEVLIQKMRNLLEANR"
     gene            complement(3233..3883)
                     /locus_tag="PLW22_11185"
     CDS             complement(3233..3883)
                     /locus_tag="PLW22_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOU75306.1"
                     /translation="MNETTMLLTLLIKGMIIGLAASIPLGPIGVICVQRTINKGKISG
                     FLSGLGAATADTLFAAIAGFSLSFIISFLEERQTIIELVGGVIVVFLGLKIFSTNPIS
                     QLKRNKRKKSNLLEDFMSVLLLTGSNPLAVFFFIALFATAGVISHDQSIWVNLIALAG
                     VFLGGALWWYILTSTVNYFRRHFRLKQLWWINKISGAVIFTLGGLAILNVIHKVFF"
     gene            complement(3959..>4488)
                     /locus_tag="PLW22_11190"
     CDS             complement(3959..>4488)
                     /locus_tag="PLW22_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU75307.1"
                     /translation="EKFLQGTANLKNGHPLDTDTDISSMIDEENAIRVEEWVNDATNA
                     GAKVLLGGKRKGSFFQPTVLTNTTNSMKVCALEVFGPVVTLEPYTDFHEAVNLINQSE
                     FGLQAGVFTNLIDEMNFAFNELRVGGVIINDVPTFRVDHMPYGGIKNSGFGREGVKYA
                     MLEMLEPRLLVKNIL"
BASE COUNT         1292 a          911 c          936 g         1349 t
ORIGIN      
        1 atcagcgttt tgggattgaa ccccaagcag caaaagtata tcgacagagt attaatgagc
       61 aaattcagca ttgttaccta tgataggttt aaggttaact actaccggtt aatgtccgat
      121 aaagtgtttg ctcgcattca accccagttc gtttacgata ggaatagcgg actttttgat
      181 gtcaaaatgc aagccatact taacaggcac cccagccttg gcttaggttt gagcctttca
      241 tccgataatg gcaatgaagg gtttttctct attacccata actggttaac cagaacatct
      301 aataccttgt acggcaatat atactttgga aaatttcaca gtagtgcaag gctaacagcc
      361 atgaaaactt tcccttcgcg aattcctgtc tcgctggtgt caacccttgt agctaaccgt
      421 tacgattacc atagtggtaa cccggttccc tttttcgagg atgtaaaacc tgcctatgcc
      481 atccagaatg agacccttgt cactattgcg ttgcgcctta gccataccag cgcactgaac
      541 acatcaatgt ttgtaaccag tggtgaaaag atggatgagt attaccaaac cgagaactac
      601 tactcattcg atatccccga taaaacccgc ttccggttct ttaagggggg aatcaggctg
      661 gaaaaaaata ccctgaacca taagcagtat gctaaccggg ggcgacatca actagtatcg
      721 atatcggggt actacggaca cgaaattcat aagccaggca caacagccat gcaactctcg
      781 gaatttgaca attaccaatc gtttatcact gcatacctcc acaatgagag ttaccatagg
      841 ctaattggaa agagtttctg gcttggcttt tacctggatg gctactggtc atcgcaacgg
      901 tttttcgata actactgggc tacaattctg tcaatgaatc agttctcgcc aaccccgcat
      961 tcgcggataa tgtttttaaa ggaacttcgc tcaagcaagt atgttgctat tggtttatcg
     1021 ccggttgttg attttggcag taatgtgagt ttccgaatag atgcttttgt ttatcagcct
     1081 tacaaagcca ttttagctaa tagcgatggt ttagcctact acggcgatgc ctttaaaagc
     1141 agggagtatc tgatgtcggc ctcagtaatt tatagttctc cggtaggccc agtaacaata
     1201 acggtatcgc attaccccag aaataagggc aagcagttct ttttgaattt gtcgtttgga
     1261 tactcgttgt ttaacccaag ggttttcgat aactaacgat ttgcctcaag taggtttcgc
     1321 attttctgga taagcacctc aatctggaaa ggcttgctga tgtaatcgtc cattccggcg
     1381 gctaaacatt cttccctgtc gcccatgagt gcattagcag ttatggctat aattggggtg
     1441 tgcgagtttg tgcttgcctc aatctcccga atctttttag ctgccgtgta gccattcatg
     1501 attggcattt gaacatccat cagaataata tcgtacttgc tggttccaaa tttatctaat
     1561 gcctctttcc cgtttacagc tacatcaatg tttttaacca gctttttaag gctgagtaat
     1621 acaatctttt ggttaatcag gttgtcctcc accagcaaaa cattggcatt ttctaatcga
     1681 acatccgttg tgggtaactt ttcaaccttg ctaacttcag gagtttcggg tacaatttca
     1741 ttgatacctt tttccttgcc ctcggcttgc tggatttctt ctttaagcgc tgtgggaact
     1801 ttaagcagcc tgatgttgaa gtttatttgt gagtcctgcg acgttagctt aacatttagc
     1861 ttgccaccat tttcgcgaat aagcttggct gcaattttta ggtcgagcat ctcaataaac
     1921 acctggttac tcagggcggt tgagccatcg gttagggttg ttgacgatac accatcggca
     1981 tggtgcagga gtaagggggt gtttgtttta atttcggtat aaatgtcaac cccatcgcca
     2041 gcttcctgca ttttattgat cttaatgtca agtattacct tttggctgct tttgttttta
     2101 atgatactct ccacaatgtt taggaagatc tgctttagct taacggggtc gcccagtagg
     2161 tttttcggaa gacgctcatc gtacttaaag gaaaaactaa cagtgcccga ttgggttgaa
     2221 aagaggcgga atgtgttgtt aagcgtattt tgaaggtcaa atggcacaag tacatcggag
     2281 tcctttggca tctcgcttat ggagagatct atcatactgt tcagagtact cataaggtta
     2341 tttgacgaag cctgaatggt gtcaatcata tctttgagtt tctcttcctg gatttggttt
     2401 gaaagtatgt ttgagattat gacaatgtta tttaatggtg ttcttatgtc gtgcgagagc
     2461 ttggcaaggt tctcagttcg ttttttaatg tcatttttaa gttcaatgct ttttatttct
     2521 aactcttgca gctgtttagt tcttatgaac catagcagcg acacagaggt taggtaaaca
     2581 ataaatccaa tgctaatgaa atatcggttt acataaaaaa tattttcggc gggtaatcca
     2641 ataacaggca ggataaatag aacaataggg agtagtactg caaatgctac aagtgtcacc
     2701 gatgctttat tgaatggggt agagctgaaa aacattagag gtaacaacat cgtaactgct
     2761 agtagcaaaa ggttgtttga acccagaagt atatttacca gtatgcctat agcaaaaatg
     2821 taggaaatgc caagaccaat actgctaaag cggcttgaac ggaaaagcat aaaaagtaaa
     2881 accagagcta ctataaaagt agaaaaatta attattgcaa caggtaaaga tgctttaagt
     2941 gaggtaatgg ctaggattat gaaaatgagc agtgaaggaa tagcaataag gtttagctga
     3001 gtaatcttat cggcaaaatc ttttgatttg tttgtatcaa caccaaacga caaaacctct
     3061 atgagtttat tggctatttt catctgaaat gattttcagt ttaaacaaca atccaaatat
     3121 aacaatattt taaattaata ggctaattta aaaactttat ataaacctat tgtgtttagt
     3181 tcaaatttac agaaaatacg ataagccgaa aagcaaagga gtaaccgtgg tgttaaaaga
     3241 acaccttgtg gattacgttt aaaatggcaa gtcctcccag cgtgaaaatt acagcacccg
     3301 aaatcttatt gatccaccag agctgcttta gcctaaaatg cctgcgaaaa tagtttacgg
     3361 tagatgtaag gatataccac caaagagctc cgccaaggaa aacgccagcg agcgctatta
     3421 gattcaccca tatactttgg tcgtggctaa ttacccctgc tgtggcaaaa agggcaatga
     3481 aaaagaaaac ggcaagcgga ttggaaccgg ttagcagaag aaccgacatg aagtcctcaa
     3541 gcaaattgct ctttttccgt ttgttccgtt taagctgcga aattggattt gttgaaaaaa
     3601 tctttaaacc caaaaaaact actataactc cgccaacaag ttcaattata gtttgtcgtt
     3661 cttcaagaaa gctgataatg aacgaaaggc taaaccccgc aatggcagca aaaagcgtat
     3721 cggcagtggc tgctcctaaa ccagaaagaa aaccgctaat cttaccctta ttgatggttc
     3781 gttgaacgca gataacccca attggtccaa gcggtatgct tgcagccaat cctataatca
     3841 ttcccttgat tagcagggtt aaaagcatgg tagtttcgtt catagatgcg gtgcaaatat
     3901 accaataatt agcaaatatc tgaccgttat gttttgggaa ttgagaaata gtgttaaact
     3961 acagtatatt cttaactaaa agccttggct ccagcatttc gagcatggca tacttaaccc
     4021 cttcgcgccc aaagcctgag tttttaattc caccgtaagg catgtggtcg accctgaagg
     4081 tggggacatc gttaatgatt actccgccta ctcgtaactc attgaaagca aaattcattt
     4141 cgtcaattag gtttgtgaaa acacctgcct gtagcccaaa ctctgattgg tttattagat
     4201 taactgcttc gtgaaaatcg gtatagggtt caagggtaac tactgggcca aaaacttcta
     4261 gcgcgcaaac cttcatgctg tttgtggtgt tggtaagaac cgtgggctgg aaaaaggaac
     4321 ctttgcgttt ccccccgagt agtactttag cacccgcgtt cgttgcgtcg tttacccatt
     4381 cctcaactct tattgcattt tcctcgtcaa tcatcgatga tatatcggtg tcggtatcca
     4441 gcgggtgacc attcttaaga tttgctgtgc cttgtaggaa cttttcgg
//
LOCUS       DAOOWJ010000157         4445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_19681_length_4445_cov_6.442825, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000157 DAOOWJ010000000
VERSION     DAOOWJ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19681_length_4445_cov_6.442825"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLW22_11195"
     CDS             complement(<1..301)
                     /locus_tag="PLW22_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75308.1"
                     /translation="MRLYETKVIKGTLLLAIFAMLFSSCLKDDDLMTADAKTGGLVES
                     ASLVQLVPKKSNSVDVELKVYLGPAIQEVKVYKQFYHIATDEASNVELLGSFSVSG"
     gene            complement(327..1877)
                     /locus_tag="PLW22_11200"
     CDS             complement(327..1877)
                     /locus_tag="PLW22_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOU75309.1"
                     /translation="MKLYRLFTLSIAVLGVVVLQSCTKDFDEINKNPNAIDINKDYPD
                     KLLANAIEDIADRVFDVWLGHEIGSCWVQHMAKVQYTDEDRYVPRNDVINMVWNGLYA
                     GPAIETQRIYEMGQRLGNRNYQAVALVLKSYIFSVLTDLYGDIPYKEALKVDSTYTPK
                     YDTQESIYLALNAKLKEANELFLVGGPKVQGDILFSGDITKWKRFANSLRLRLLMRMS
                     NRPEYLETIRNEIDGIVSDPVTYPIISNNSQNAALIYLGTYPNNHPLNETYKTRDDHR
                     VSKTLIDAMYNPNNHDHPDWRIVVYAETPQAGGYWIGLPNGLTSAKAAAYLGNGIKRT
                     SKLGEYFRKADAPGMFMSRAEVCFLLAEAKHRGLLTFGTETAQDYYELGVKESYRQFA
                     NEIIENVPYYYDLGGASRTIDDYFNDFLANSGKWEVGKELEKIYTQKWVAMFDQGLQS
                     WFEWRRTGYPQLTPAEDGLNGGRIPQRLRYPSDEYSRNGTNLAAAVARQGADNLNTRV
                     WWDVADNN"
     gene            complement(1897..>4445)
                     /locus_tag="PLW22_11205"
     CDS             complement(1897..>4445)
                     /locus_tag="PLW22_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU75310.1"
                     /translation="VLNNTNFGGTYTEGVNRGSGIADINPNDIESVTVLKGPNAAALY
                     GSRAANGVIIIKTRSADTDKRFGVSFNSTTTFERPLRLPDFQNVYGQGTNGQFEFYDG
                     KGGGINDHVDESWGPKLDIGLMIPQWNSPVVGGVRQPTPWVSHPDNVKNFFELGKTFS
                     NNIAIESAADNYAVRFSYTNSMQTGMIPNTDQEKNSVNLNATASPYKFLAFTANATYI
                     NTYSKNLPAYGYSGQNVMQQFMWFGRQVDVADLKQYTYPDGSKRNWNYSYHNNPYFTL
                     YENLNGIDRDRLLGQATSTIKFTDWLSLKTGIGVDYYTNYNNARSAYGDIESPYGFLS
                     ESKRTFREVNTDFMFMINKSFTEKLAFSFNLGGNRMEQFTQSLSAYTTELSVPDVYTF
                     ENARVPVVTMNYITKKRINSLYFNGQFIWKNALFFDFTGRNDWSSTLPAGSNAYFYPS
                     VNVSAVLTDLFDIQSRKLSFAKLRAGWAQVGADTDPYQLQPVYRFDDGWNSGTKLAQI
                     YIPNDLPNPNLKPQRTNSFEIGADLRFFMNRLAFDLTYYSQSTYDQIIRMPISATTGY
                     TSTIVNAGQIDNRGVELSVNGIPVKTRSGFEWNVGVNFSRNVNKVRKLAEGLDQYVLG
                     GYWSLDVLAIPGEPFGVLYGYDFMRNDEGKVIHIDGLPLQGDIKKLGNYTPDWVAGVT
                     NSVRYKNLTFSALVDARWGGEIYSMTNTWGRYAGVLKETLKGREGGIVGVGVMPDGQG
                     RWTENDVVVSAEEYNKAAYNNNIAYSSIFDASFVKLREVQIGYTIPKLGKSSIRNISI
                     SLVGRNLAILYARVPHIDPETAFSNSNVQGLEFGQHPTARSIGVNLSFKF"
BASE COUNT         1211 a         1050 c          912 g         1272 t
ORIGIN      
        1 ggccacttac actaaaactg ccgagtagtt caacattaga ggcttcgtcg gttgcgatgt
       61 ggtaaaactg cttgtaaacc ttaacctctt gtatggctgg tcccaaataa accttaagtt
      121 cgacatcaac actatttgat ttctttggta ccagttgcac caacgatgcc gactcaacca
      181 aaccaccagt ttttgcatcg gcagtcatta aatcatcatc cttcaggcag gaactaaaaa
      241 gcatggcaaa aattgccagg agcaatgttc cctttatgac ttttgtttca tataatctca
      301 tactctaatc aattaatgga gttcaactaa ttgttatcgg caacatccca ccataccctg
      361 gtatttaaat tatcggcacc ctggcgggca acagctgcag ccaggttggt accgttacgg
      421 gaatactcat cggaagggta acgtaagcgt tggggtattc ttcctccatt taatccatct
      481 tcggccggtg ttagctgggg atagccagtt ctccgccact caaaccatga ctgtaagccc
      541 tgatcaaaca tggcaaccca tttctgggtg taaattttct caagttcctt accaacttcc
      601 cacttaccac tatttgcaag aaaatcgtta aaataatcgt caatggttcg tgaagcacca
      661 cccaaatcgt agtagtaagg cacgttttca ataatttcat tggcaaactg cctgtacgac
      721 tccttaacac ccagttcata gtaatcctga gctgtttcag ttccaaatgt taacaaacca
      781 cgatgtttag cttcagccaa caagaagcaa acctctgcac ggctcatgaa catacccgga
      841 gcatcagctt tacggaagta ttctcccagc ttagaggttc gtttaatacc attgcccagg
      901 taagcggcag cctttgccga tgtcaaacca ttgggtaacc ctatccagta acctcccgcc
      961 tgaggtgttt cagcgtaaac tacaattcgc caatcgggat gatcgtggtt attggggttg
     1021 tacattgcat caattaacgt tttactaacc ctatgatcat cgcgggtttt gtaagtttcg
     1081 tttaacgggt ggttattggg gtaagtccca aggtaaatca atgcagcatt ctgtgaatta
     1141 ttgctgataa ttggataggt aacaggatca gaaacaatgc catcaatttc atttctgatg
     1201 gtttcgagat attcaggcct gttggacatt cgcataagca aacgtaatcg tagcgagttg
     1261 gcaaaacgtt tccatttagt aatatcaccg ctaaatagaa tatcgccttg tacctttggg
     1321 ccaccaacca gaaataattc attagcttcc ttaagctttg catttaatgc aagatagatt
     1381 gattcctgcg tgtcgtactt gggcgtgtag gtgctgtcaa cctttaatgc ctctttatag
     1441 ggaatatctc cataaaggtc ggttagcacc gaaaaaatgt aagattttag caccagcgcc
     1501 actgcctggt aattccggtt ccctaacctc tgccccatct cataaattcg ttgggtttca
     1561 atggcaggcc ctgcatacaa tccgttccag accatgttaa taacatcgtt gcggggaaca
     1621 tacctatcct catcggtgta ctgcaccttt gccatgtgtt gcacccagca tgagcctatc
     1681 tcgtgcccca gccatacatc aaatacccta tcggcgatat cctcaatggc atttgccagt
     1741 agcttatcgg ggtaatcctt gttgatatca attgcgttgg ggtttttgtt gatctcatca
     1801 aaatcctttg tgcacgactg caagacaact acccccagta cagctatgct taacgtaaat
     1861 aatctatata atttcatacc tctttttttt atcaagctaa aacttaaaac tcaaattcac
     1921 tcctatgctg cgggctgtag gatgttgacc aaactctaac ccctgaacat tggagttgct
     1981 aaatgcagtt tcggggtcaa tatggggcac ccttgcataa agtatggcta agttacgacc
     2041 cactagcgaa atggaaatat tccgtatgga gcttttacct aatttgggta tggtgtagcc
     2101 tatttgaacc tccctaagct ttacaaagct tgcatcgaaa atgctggagt aggcaatatt
     2161 gttattatag gcggctttat tgtactcttc agccgagaca accacgtcat tctcagtcca
     2221 tctaccctgt ccatctggca ttacgccaac acctactatt cctccctcac gacccttaag
     2281 agtttccttc aatacccctg cgtatcggcc ccaggtattg gtcatggagt agatctctcc
     2341 accccaacgt gcatcaacaa gagcactaaa ggttagattt ttataacgaa ctgagttggt
     2401 tacacctgca acccaatcgg gcgtgtagtt gccaagcttt ttgatatcgc cctgcaaggg
     2461 taatccgtcg atatgaatta ccttaccctc atcattcctc ataaaatcgt acccgtaaag
     2521 tacaccaaaa ggctcgccgg gtattgctag cacatcaagg ctccagtatc cacccagcac
     2581 gtactgatca aggccctcgg caagttttct taccttatta acgtttcgcg agaagtttac
     2641 gcccacgttc cactcaaaac ccgaacgggt tttaacagga attccattca ctgaaagctc
     2701 aactcccctg ttatcaattt gaccggcatt aactattgtt gaagtataac cggttgttgc
     2761 gcttatgggc atacggataa tttgatcgta ggtgctttga gagtaatacg ttaggtcaaa
     2821 tgcaagccga ttcatgaaaa accgtaggtc ggcaccaatt tcaaacgaat tggttctttg
     2881 gggtttaaga tttggatttg gcaagtcgtt gggaatataa atttgtgcaa gcttggtgcc
     2941 ggaattccaa ccatcatcga acctatacac gggttgtagc tggtagggat cggtatcggc
     3001 gccaacctgt gcccaaccgg cacgtagctt ggcaaagctt aattttcttg attgaatatc
     3061 gaacaagtcg gtcagcacgg ccgatacatt taccgatgga taaaagtatg cattactacc
     3121 cgctggcaaa gtgctcgacc agtcgttacg ccctgtaaaa tcaaagaata atgcattttt
     3181 ccaaataaac tgaccgttaa aatataaact gttaattcgc tttttggtaa tgtagttcat
     3241 ggtaacaacg ggtaccctgg cattctcaaa ggtgtaaaca tcgggtacac taagttcagt
     3301 agtataagcg ctcagcgatt gggtgaactg ttccatccgg ttgcccccca aattgaacga
     3361 gaaagcaagt ttttcagtaa acgacttatt gatcatgaac ataaagtcgg tattaacctc
     3421 acggaacgtg cgctttgact cgctcagaaa accataggga ctttcgatat cgccataagc
     3481 ggaacgcgca ttgttatagt ttgtgtagta atcaactcct atacctgtct ttaggcttaa
     3541 ccagtcagta aactttatgg ttgatgttgc ttgacccaac agcctgtccc tatcaatacc
     3601 attaaggttc tcataaaggg taaagtaagg attattgtgg tagctgtagt tccagttacg
     3661 cttgctacca tcgggatacg tgtactgttt caaatcggcc acatcaacct gtcggccaaa
     3721 ccacatgaac tgctgcataa cattctgccc gctataaccg tatgcaggaa ggtttttact
     3781 ataggtatta atgtacgttg catttgcagt aaatgccaga aatttataag gcgatgctgt
     3841 agcattcaag ttaacactat tcttttcctg gtcagtatta ggaatcatgc ccgtttgcat
     3901 ggagttagtg taggaaaagc gaactgcata gttgtcggca gcgctctcaa tggcaatgtt
     3961 gttggaaaaa gttttcccaa gttcaaaaaa gttcttcaca ttatccgggt gcgataccca
     4021 gggtgtgggt tgacgaacac cgcccacaac aggcgagttc cactgcggaa tcatcagccc
     4081 aatatcgagt tttggccccc agctctcatc aacatggtcg ttaatgccac cacctttccc
     4141 gtcgtagaat tcaaattggc cattcgttcc ttgtccataa acattctgaa aatcgggtaa
     4201 acgcaaaggc ctttcgaatg ttgtggttga gttaaagcta acgccaaagc gtttatcggt
     4261 atcggctgaa cgggttttaa taatgataac cccattggca gctcgcgatc cataaagcgc
     4321 tgcagcattg ggccctttaa ggacggtaac gctctcaata tcgtttggat tgatgtcggc
     4381 aataccgctt ccgcggttta ccccttcggt gtaagtacca ccaaagttgg tgttgttaag
     4441 cacaa
//
LOCUS       DAOOWJ010000158         4441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_19703_length_4441_cov_8.680347, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000158 DAOOWJ010000000
VERSION     DAOOWJ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19703_length_4441_cov_8.680347"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLW22_11210"
     CDS             <1..498
                     /locus_tag="PLW22_11210"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOU75311.1"
                     /translation="GVTLLATIGTMVYGYYKQKQFADNIARNPQEQMEQQAKFLRDRL
                     NLTPSQEDIFRKSRNEFHEKTVDINRKLQRVSIEIINELSSPEPDLKLIDSLVEQYGE
                     LHKAEKKVMVDHLMEIKSACTPEQFQKFKRIVEHSQRRMMQRHRQRFQNERMRRGDSI
                     PPRIQ"
     gene            530..1021
                     /locus_tag="PLW22_11215"
     CDS             530..1021
                     /locus_tag="PLW22_11215"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOU75312.1"
                     /translation="MKRIGYIMMVVLLLVSTMGMAQGPRFRGGDGNTMQTVGKGYCYL
                     NLTPEQEQKINDLRVKHLKEITPLRNELSEKQAHLRTLESADKPDMAAINKTIDEITS
                     IKAKLMKARVAHRAEVSQLLTDEQRVLFNARGQKGKGMRGHRGNMRGGYGMGMGLGPC
                     MGN"
     gene            1403..2257
                     /gene="speB"
                     /locus_tag="PLW22_11220"
     CDS             1403..2257
                     /gene="speB"
                     /locus_tag="PLW22_11220"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOU75313.1"
                     /translation="MIHYGDLPKPYCEFETSKVAIVPVPYDGTSTWIKGADKGPKALL
                     DASANMEIYDIETDTEVYKIGIYTDKPITEDSSPEAMVEAVKERTLSLIDKGKFTVII
                     GGEHSVSIGTIQAHAQKYKNLSVLQIDAHTDLRDSYEGSKNNHACVMARAKELCPIVQ
                     VGIRSMDSGEKANLDPNRVFWAHKIVNCDDWMDKAIDLLTDNVYLTIDLDGFDPSILP
                     STGTPEPGGLLWYPTLKFLRKVIEKRNLVGFDIVELCPNTDDKASDFLAAKLLYKILT
                     YKFLNQLK"
     gene            2297..2494
                     /locus_tag="PLW22_11225"
     CDS             2297..2494
                     /locus_tag="PLW22_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75314.1"
                     /translation="MNRCKCIKVPESNNGQGKFKLNAYYEFDYIPPIKDNASYYRVFS
                     LEDNISENFSIKAFNEHFRKY"
     gene            complement(2548..3258)
                     /locus_tag="PLW22_11230"
     CDS             complement(2548..3258)
                     /locus_tag="PLW22_11230"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOU75315.1"
                     /translation="MEENQVIAMPRIGDPAPEFKAMTTQGEINFPHDYKGSWVILFSH
                     PADFTPVCTSEFMTFASMEQKFNEANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMKN
                     VEVTFPLIEDITMDVAKKYGMIQPGESNTKAVRAVFFIDPKGIIRTIIYYPLSLGRNF
                     EELYRVLIALQTADEFGIATPADWQPGDDVIVPPAGSCGTAKDRMEGKDPNVKCFDWF
                     FCTKKLDKDTVMKKILKK"
     gene            complement(3288..3800)
                     /locus_tag="PLW22_11235"
     CDS             complement(3288..3800)
                     /locus_tag="PLW22_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOU75316.1"
                     /translation="MGKEGIRIASVDVEKLLEMLNAALAEEWLAYYQYWIGAKVMEGP
                     MRSEIEPELLLHASQELNHAVMVADRIIQLGGTPLLHPNDWFKHAGCSYDAPVDPYIE
                     AILEQNLNGERCAIRRYNEIATFTNGKDHMTHQMATQILAEEIEHENDIEDWITDINR
                     MKEEIKKFKL"
     gene            complement(3804..4247)
                     /locus_tag="PLW22_11240"
     CDS             complement(3804..4247)
                     /locus_tag="PLW22_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOU75317.1"
                     /translation="MNYDELTKLLSEKGLKVTPQRVAVLEALYEIMNHPSAEEVRQKL
                     HSRFPSIAIGTIYNILDTFCEHGIIRKVKTDSDFVRYDVDMEKHHHIYCESCNHIENY
                     HDPELDELLKAYFDRKQIPHFQIKEFSLQIIGNYKATNYKPNQNK"
BASE COUNT         1334 a          966 c          915 g         1226 t
ORIGIN      
        1 ggagtaacct tactggctac aataggcaca atggtttacg gctactacaa acaaaaacaa
       61 tttgctgaca atatagcccg taacccgcag gaacaaatgg aacagcaagc caagttttta
      121 cgcgataggc taaatcttac acccagccag gaggatattt tcagaaaatc tcgcaacgag
      181 ttccatgaaa aaacagttga tattaacaga aagcttcaaa gggtttcaat tgaaattatc
      241 aatgagcttt cgtcgcctga accggattta aaacttatcg attcactggt tgaacagtat
      301 ggcgaactcc ataaggctga aaaaaaggtt atggttgacc acctgatgga gatcaagagt
      361 gcatgtactc ctgaacagtt ccaaaaattt aaaagaattg tggagcattc gcaacgccga
      421 atgatgcaac gacatcgaca aagatttcaa aacgaacgca tgcgtagggg cgattctatt
      481 ccaccccgaa ttcaataaaa ctgacgttta actattaaaa tttaacacaa tgaaacgtat
      541 tgggtacatt atgatggtag tgctactttt agtatctaca atgggtatgg ctcaagggcc
      601 aagattccga ggtggcgatg gaaataccat gcaaacagtt ggaaaaggtt actgctacct
      661 aaacctaacc cctgagcagg agcaaaaaat caacgacctt cgggttaaac acctaaaaga
      721 aataacaccg ctccgtaacg agcttagcga aaagcaagcg catttgagaa cacttgaatc
      781 ggctgataaa cccgatatgg cagccattaa caaaacaatc gatgagatta cctcaatcaa
      841 agcaaaactt atgaaagcac gtgtagccca cagggctgag gtttcgcaac tactaaccga
      901 tgaacaacgt gtcctgttta acgcacgtgg ccaaaaaggt aagggcatga gaggacatcg
      961 cggtaatatg cgcggcggtt acggaatggg aatgggctta ggaccatgca tgggcaacta
     1021 gccaatgggg ttctaacctc ctaaaaaaag gcactcataa tgggtgcctt ttttgttgtt
     1081 cgtgaaaact acctcacacc tgccccccct gagagggaat ggaagggaaa aaagcaaaca
     1141 tgagcataaa agcctaaccc tcgaattgga ttacccttga tataatatta tgcccgtacg
     1201 gggctactaa cctcaaactt gaaagagatt cctcgcattt gctaggaatg ataaatcccc
     1261 tggcatcctt agtacctttg tcatgctgag ctaagtcaaa gcttctcttt gtaccttcta
     1321 cacattttta atgagattcc attacctaaa ctaaaattgt ttttttttag tacttttgcg
     1381 ggaaatttga aagcatatac ctatgattca ctacggcgat ttacctaaac cctactgtga
     1441 atttgaaacc tcaaaagtgg ctattgttcc agtaccctac gatggaacca gcacatggat
     1501 taagggtgcc gacaaagggc ccaaggccct gctcgatgcc tcggccaata tggaaatata
     1561 cgatattgaa accgataccg aggtttacaa gataggtatt tataccgata agccaataac
     1621 agaggatagc tcacccgaag caatggttga agcggttaaa gaacggacgc ttagcctcat
     1681 tgataaaggt aaatttacag tcattatagg tggtgaacac agtgtaagca ttggaactat
     1741 acaggctcat gcccagaaat acaaaaacct ttcagtgctt caaattgatg cccataccga
     1801 cctgcgcgat tcctacgagg gaagcaaaaa caatcacgct tgcgttatgg ctcgggcaaa
     1861 ggaactttgc ccaattgttc aggtaggtat tcgcagtatg gactcaggcg aaaaagccaa
     1921 ccttgacccc aaccgggtgt tctgggctca caaaatagtg aactgcgacg actggatgga
     1981 caaggctatc gatttgctaa cagataatgt ttacctaacc atcgacctcg acggttttga
     2041 cccctccata ttgccaagta caggaactcc tgagcccggc gggcttttgt ggtaccctac
     2101 cctcaagttc ttaagaaagg ttattgaaaa acgcaacctg gtagggttcg acatcgttga
     2161 gctatgtccc aacaccgacg ataaagcatc ggattttctg gcggccaaac ttctttacaa
     2221 aatactaacc tacaagtttc tgaaccagct taagtagtta aagcaaatat caacctaaac
     2281 ttaaaaaacc taacctatga accgctgtaa gtgtattaaa gttccagaat cgaacaatgg
     2341 gcagggtaaa tttaaactca atgcctacta cgagttcgat tacatcccgc ccattaagga
     2401 taatgcctcg tattacaggg tgtttagcct tgaggataac attagtgaaa attttagcat
     2461 caaagcgttt aacgaacact tcagaaaata ctaaaaaaaa acggctcaaa tccttgagcc
     2521 gtttttcgtt taaagtatat tccctagcta tttcttaagg attttcttca tcaccgtatc
     2581 cttgtccaac tttttagtgc agaagaacca atcgaagcac ttcacattgg ggtctttacc
     2641 ttccatcctg tccttagcag tgccacagga accggcagga ggaacaatta catcatcacc
     2701 aggttgccaa tcggcagggg tagcaattcc aaactcatcg gcagtttgta aagcgatgag
     2761 tacgcggtat agctcctcaa agttacggcc caagctaagt gggtagtaga tgatggttcg
     2821 gataataccc ttgggatcaa taaagaatac cgcacgcact gccttggtgt tgctctcgcc
     2881 gggttgaatc ataccgtatt tcttggcaac atccatggta atatcctcaa ttagcgggaa
     2941 tgttacctct acgtttttca tacccttgta ttcaatcttc tccttgatgg tacgcagcca
     3001 tgcaatgtgg ctatataaac catcaaccga aagccccaca agcttacagt tagcctcgtt
     3061 aaatttctgc tccatggagg caaaggtcat gaactccgat gtgcatactg gggtgaaatc
     3121 ggccggatgg ctgaatagga ttacccagct tcccttgtaa tcatgcggaa agttaatttc
     3181 accctgggtt gtcatggcct tgaactcagg cgctgggtcg ccaatgcggg gcattgcaat
     3241 tacttgattt tcttccattt ttgtttattt taaggtttga caaattatta cagcttaaat
     3301 ttcttaatct cctccttcat gcgattgata tcggtaatcc aatcctcaat atcattctca
     3361 tgttcaattt cctcggcaag tatctgcgta gccatttggt gggtcatgtg atccttacca
     3421 ttagtaaagg tggctatctc gttgtacctt cgaatagcac aacgctcacc gttcaggttc
     3481 tgttccagaa tggcttcaat gtaagggtca acgggagcat cgtaactgca accggcatgc
     3541 ttaaaccaat cgttgggatg tagcaatggt gtgccaccta gctgtatgat acgatcggct
     3601 accataacgg catggttgag ttcctggctg gcatgtagta acaattcagg ttctatttcg
     3661 cttcgcatag ggccttccat aacctttgca ccaatccagt actggtagta agcaagccat
     3721 tcctcggcta gtgctgcgtt aagcatctca agtaattttt ctacatcaac cgatgctatt
     3781 ctaattcctt cttttcccat agtttatttg ttttggtttg gtttgtagtt agttgcttta
     3841 taatttccaa tgatttgtag gctaaactct ttgatctgga aatgtggaat ttgttttcta
     3901 tcaaagtatg cctttaggag ctcatcaagc tcaggatcgt gatagttttc aatatggttg
     3961 caactttcac aatatatatg gtgatgcttt tccatatcaa catcgtacct aacaaaatcg
     4021 ctatcggttt ttacctttct aataatgccg tgttcgcaga atgtatccag gatattgtat
     4081 atggttccta tggctatgct gggaaaacgg ctatgcaatt tttgccttac ctcctcagca
     4141 gaggggtgat tcataatctc atacaaagcc tcaagcacgg caaccctttg cggggttacc
     4201 tttaatccct tttcggaaag caacttggtt agttcatcgt aattcatcct atataggaat
     4261 tattcttgtt tagtagaaca taaatttaca aaaaaggttt aggcaagtca ataatatttt
     4321 tgaaaatctt taacccaagt gccatgaatt aacttttaga ggaatgagtc ggaataggca
     4381 gaatatgatg tctagtcctg aaataagtct acaggttaga aatagattta agcgacacac
     4441 a
//
LOCUS       DAOOWJ010000159         4367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_20120_length_4367_cov_8.021568, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000159 DAOOWJ010000000
VERSION     DAOOWJ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20120_length_4367_cov_8.021568"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..469
                     /locus_tag="PLW22_11245"
     CDS             89..469
                     /locus_tag="PLW22_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOU75318.1"
                     /translation="MAGHKGSKYYDVFLDYTIVLRERNTNQSILTQEQLCLLLNIDDL
                     ESIAASAKKMDISYRKAWELVRRAEEELGFPLVSKSRGGSDGGKSELTSDGKQLVDAY
                     RQLRNEFNESVKRVVKNFFQTINR"
     gene            473..1417
                     /locus_tag="PLW22_11250"
     CDS             473..1417
                     /locus_tag="PLW22_11250"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU75319.1"
                     /translation="MRNLLAILLLSILLSNCSSRQNDQNSLTIFHAGSLSLPLRAAID
                     SFIRYNPEVTINPEAAGSVECARKITDLNRPCDLFFSADYKVISSLLVTGHADFFIPF
                     ATNRMVIAYSPKSKHADIIDSLNWPTILLRSDVAYGRSDPNSDPCGYRTLLLFQLAEK
                     HYRMVGLAESLEGKDNQFIRPKEVDLVAMLETSAIDYLFIYESVVKQHKLNYIPLPDS
                     INLGNFSLAQHYGSVQVKIRGNSPNDSITIAGEPIVYAFTIPKNAPNPLLASKFATYF
                     LSDTGGMALITKMGQQSLIPFSCTSCGSVPDDFKEFIR"
     gene            1428..2216
                     /locus_tag="PLW22_11255"
     CDS             1428..2216
                     /locus_tag="PLW22_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU75320.1"
                     /translation="MSKSGVNNFSTFSLVLTLLGAFILLFIIAPLLGMIVNTPFKDVA
                     ATTADSQVLSSIWLSIWVAMLTSILFAFPAIPLAYILARYDFPFKSVINGIIDLPVVI
                     PHSVVGIALLGLIAGDGLVGKSLDVFGVRLIGTPFAIALAMAFVSLPFLINSARDGFI
                     QVPVRLEQAALSLGATPFQVFFTISLPLAWRNILSGLVMMFARGMSEFGAVVIVAYHP
                     MVASVMIYDRFTAFGLKYSRPAAFIFLVVSLIVFILLRFISRKR"
     gene            2221..3282
                     /locus_tag="PLW22_11260"
     CDS             2221..3282
                     /locus_tag="PLW22_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU75321.1"
                     /translation="MLVVDQISKHFKLFSLREITFKVSQGDYFMFLGPSGSGKSLLLQ
                     IIAGLVKPDSGRILFNGIDIAKSPAGKRNVGILFQDLALFPHLSVFDNIAYPLKLRKY
                     SSFAVKQRVVELAEQFSISHILNQGVTSLSGGEKQRVALARTLAFNPPILLLDEPLSA
                     IDSQLHLDLGVLLRSINRTGTTIIHVTHNYQEAISLASKVGVLHNGQLVQVGEPVEVF
                     QNPRSEFVANFTGAKNFYRVKGVRATPDGLIYAELPFGETIAFYANPAYSNGFVCFPE
                     ESVVLSTQAENQSAINVFSGSVIDIFAQRFGYEVVIDCGFKVFALITKESLEKLKISP
                     GIKLYAAVKANAVRFIPEI"
     gene            complement(3292..4188)
                     /locus_tag="PLW22_11265"
     CDS             complement(3292..4188)
                     /locus_tag="PLW22_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU75322.1"
                     /translation="MAYLLQSGTFALEMVWDIMKYKSTWYFLLLLCLFALTACVKPGN
                     YGMRDRYVVTTRSLYVRTAPSTVSEIIGTLRQGDTVVAVVSDKYWIMVRNGYATGYVS
                     TDYLSKIEYPSTPKFLKLIERLANWQNWYFWVIAIALLFIWVIAEELVIEAKQFLKKR
                     HDFNIKEVIVSYVTFFVVGLLSGIIYIYWKDYFIESITKGISANNFGTDIVTISIWIQ
                     LSLLAISILYDYLGSIFKTGLRFGTLLSFMDFLFGLFIFSTTFFLVIALSYYAIAFMI
                     VFFTSRYIHVVYQNGIKHRLRK"
BASE COUNT         1194 a          897 c          905 g         1371 t
ORIGIN      
        1 gttgccttta caagtaaaaa aattaggggg aataatcccc cttttgtata tttgccgata
       61 ggaatgcaat tgcatatcag ttggggttat ggccggacat aagggatcaa aatactacga
      121 tgtttttctg gactacacaa tagtgttgcg cgaacgtaac actaaccaat cgattcttac
      181 ccaggagcaa ctttgtttac tgttgaacat cgatgatctg gaatcaattg cagcatcggc
      241 aaaaaaaatg gatatcagtt accgtaaggc atgggagttg gttcgtagag cggaggagga
      301 acttggtttt ccccttgtga gtaagtcgcg gggtggcagc gatggcggta aatcggagct
      361 tacttccgat ggcaagcaac tggttgatgc ttatcgccag ctccgcaacg agtttaatga
      421 atccgtaaag cgtgttgtta aaaacttttt tcaaaccatt aataggtgat ttatgcgaaa
      481 tttgttagcc attcttttgt tgtctatact tttatctaat tgttccagta gacagaatga
      541 tcaaaattct ctaacaatct ttcacgcagg tagtttatcc ctacctctca gagcagcgat
      601 tgatagtttt atccgatata atccggaagt aacaatcaat cccgaagcgg ctggaagcgt
      661 agagtgtgcc cgtaaaatca ccgaccttaa ccgaccctgc gacctgtttt tctcggccga
      721 ctataaggtg attagcagtt tactggttac tgggcatgcc gattttttta ttccctttgc
      781 aactaaccgc atggttatag cctattcacc taaatcaaaa catgccgata taatcgattc
      841 attaaattgg cctaccattc ttttacgttc cgatgtggct tatggccgga gcgatcccaa
      901 ttccgaccct tgcggttata gaaccttgct tctttttcaa ctcgctgaaa aacactaccg
      961 tatggttggc ttagctgaat cactcgaggg gaaggataat cagtttattc gccctaaaga
     1021 ggtggatttg gtggccatgc tcgaaacatc tgccattgat taccttttta tatatgagtc
     1081 ggtggttaag cagcataagt taaattacat tcctttgcct gacagtataa atcttggaaa
     1141 cttttcccta gcacagcact atggctctgt tcaggtgaag ataaggggga attcccccaa
     1201 cgattcaata accattgcag gcgaacctat agtatatgca tttacaattc ccaaaaacgc
     1261 accaaatccc cttttggcaa gcaagtttgc aacctacttt ttgtccgata ctggtggaat
     1321 ggcgctaatt accaaaatgg ggcaacagtc acttattccg tttagctgca ccagttgtgg
     1381 ttcggtaccc gacgatttta aagagtttat tcgctaaatt attgttgatg agtaaaagcg
     1441 gagtaaacaa cttttcaacg tttagtcttg ttctaaccct tttgggtgcc tttatattgc
     1501 ttttcattat tgctccatta ctggggatga tagtcaacac tccatttaag gatgttgccg
     1561 caactacagc cgacagtcag gtgctaagtt ccatatggtt atccatttgg gtagctatgt
     1621 taacctccat tttatttgca tttcccgcta ttcctttagc gtatattctt gcccgttacg
     1681 atttcccgtt taaaagtgta ataaacggaa taattgattt gcccgtggtt attccccatt
     1741 cagtagttgg tatagctttg ctcggattaa tcgctggcga tggcttggtt ggtaaatctc
     1801 ttgatgtctt tggggtaagg ttgattggaa ccccatttgc cattgcattg gcaatggcat
     1861 ttgtcagctt gccatttctt attaattcgg ctcgcgatgg cttcatacag gttcctgtga
     1921 ggttggagca ggcagcatta tccctgggcg caaccccctt tcaggtattt tttaccatct
     1981 cattgccgct tgcttggcgt aacatcctaa gcgggctggt aatgatgttt gcccgtggca
     2041 tgagtgagtt tggtgctgta gttattgttg catatcatcc catggttgcc tcggttatga
     2101 tttacgatag gtttacagct tttggattaa agtattcaag gcctgccgct tttattttcc
     2161 ttgttgttag ccttatagtc tttattttac tgcgttttat ctcccgcaaa cggtaggatt
     2221 atgctggttg tagatcaaat atcaaagcat tttaaattat tttcacttag ggagataacc
     2281 tttaaggtgt cgcaaggcga ttactttatg tttttaggcc cgtctggttc tgggaagtcg
     2341 cttttactgc aaataattgc cggccttgtg aagcccgata gcggtagaat cctttttaat
     2401 ggtatcgata tagctaagag tcctgcaggt aaacgcaatg tagggattct ctttcaggat
     2461 ttagcccttt tccctcatct tagtgttttc gataacattg cttacccttt aaaactccgt
     2521 aaatacagtt cttttgcagt taagcaaagg gtagttgaac ttgcggaaca gttttctatt
     2581 tcacatatcc ttaaccaggg tgtaacaagt ctctcaggag gtgaaaagca gagggttgct
     2641 cttgcccgta cactcgcatt taatccgcca attctattac ttgatgagcc tctatctgcc
     2701 atagattctc agctccatct ggatttaggt gttctgctta ggtcaattaa ccgtacagga
     2761 acaaccatta ttcacgttac ccataactac caggaggcta tttcgctagc aagcaaggtg
     2821 ggagtcttgc acaacggaca gttggttcag gttggcgaac ccgttgaggt attccaaaac
     2881 cctcgttccg agtttgtggc taactttaca ggggctaaaa acttctatag ggtaaaggga
     2941 gttcgggcta ctcccgatgg tcttatttac gccgagttac cttttggtga aaccattgca
     3001 ttttatgcta acccagctta ctctaatggc tttgtctgtt tccccgagga aagcgtagtg
     3061 ctatcaacac aggccgagaa tcaaagtgca ataaatgttt ttagcggaag cgttatcgat
     3121 atttttgccc agcgttttgg ctacgaggta gtgattgact gcggttttaa ggtatttgca
     3181 ctaataacta aagaatcatt ggaaaagcta aaaatatccc cgggtattaa gctttatgca
     3241 gcggttaagg ctaatgcagt gaggttcatc cctgaaattt agttccaaag gctatttccg
     3301 taacctgtgt ttaataccat tctggtaaac cacatgtatg tatcgcgaag taaaaaatac
     3361 tatcataaac gcaattgcgt agtagcttaa ggctataaca aggaaaaatg tggttgagaa
     3421 aataaataac ccaaacagaa agtccataaa tgatagtaaa gttccaaacc ttaagccggt
     3481 tttgaaaatg gagccgaggt aatcataaag tatgctgatt gccaaaagcg ataattgaat
     3541 ccatatggag atggtaacga tgtcagttcc aaagttgtta gccgaaatac cttttgttat
     3601 actctcgatg aaataatcct tccagtaaat gtaaatgatg cccgatagta ggccaaccac
     3661 aaaaaaagtt acgtacgaca caataacctc cttgatatta aaatcgtgtc gtttctttaa
     3721 aaactgtttt gcttctatta caagttcttc agctataacc cagataaaca gaagtgcaat
     3781 ggctataacc cagaagtacc aattttgcca gttcgctaaa cgctcaatta gcttcaaaaa
     3841 tttaggtgtg ctgggatact caattttact taggtaatcg gttgaaacgt aacctgttgc
     3901 atacccattc cgcaccataa tccagtattt atccgatacc actgcaacta cagtatcccc
     3961 ctgcctaagg gttccaatta tttccgatac ggttgatggt gcagtgcgta cgtatagcga
     4021 acgggtagtt actacatacc tgtcgcgcat gccataattc cctggtttaa cacatgccgt
     4081 taaggcgaat aggcaaagta ggagtaaaaa ataccaagtt gacttgtact tcattatgtc
     4141 ccaaaccatt tccaatgcaa atgtaccact ttgcaacaaa taggccattg tatatagcac
     4201 tttttaataa taaaaaggta agaattgttt tttttcctca catcaaaccc ttcgtggtaa
     4261 aggacttaag ctgctttttt gtttcggtta gccaaatttt caccattata agctgtctag
     4321 tcctgaaata agtctacagg ttagaaatag atttaagcga cacacat
//
LOCUS       DAOOWJ010000160         4348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_20226_length_4348_cov_9.437922, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000160 DAOOWJ010000000
VERSION     DAOOWJ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20226_length_4348_cov_9.437922"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLW22_11270"
     CDS             complement(<1..1316)
                     /locus_tag="PLW22_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOU75323.1"
                     /translation="MRIGKLNISADKIKMRIGAKLLLWVLTTSAAIMIVIGSFISIRV
                     NSIARDNAIKIAQAEAQKSAFRLKSELDLDMGFSRALAHALYIYPKFDTVTLDSIFFN
                     ILRNQVSNNPRYLTVWYSIEYSAFRPGYTKDYGRRSVTAYLSNGQVGIDIEHKNITGD
                     IVTSNYYASKTSNCEMLLDPYTFEYGGKEVLATSLSVPVRVKGKFAGLGGVDISLEKF
                     QTDIEKIKPYPGTNVSLIAGDGRIIADSNPENAKEYFANIYPELESKFALTQKVKDKQ
                     NHFFFSYNSGMEYLNILSILQPGESPNSWGLILSIPMSEIVKDARRSVFSTILVFILG
                     IILQAFAIWYISARISKPIKRTTELLNSIAQGDIDVNKKIYLHTGDELEEMSRSANKL
                     IDGLNYTEQFAREIGEGKLDTEFKLLSEKDKLGKALTEMQRSLIKAR"
     gene            1502..3793
                     /locus_tag="PLW22_11275"
     CDS             1502..3793
                     /locus_tag="PLW22_11275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU75324.1"
                     /translation="MRILNIKKCITFLVFLAVSIFAGLHAQEIDSLLSLSEHYRTVNP
                     DSAVSVARQALAKVDAVNQPDIAARVYFSLGYAYYNRNDFELALEHFNKSLMLAEQSD
                     NVQLKALLHNRIGNVYQLKSYYSQAIDEYKKALDYNYKIGSKVEIARTLVNIGTVFAI
                     TGNFSIALQYMLDALEIFEQSGETEGMAWTSLSISRLFSRLEINERALQYAENALNHY
                     RRLNNQTGITLAQTELANIYYHTGMHDKALDLLGKVIDENSRTRNIHAQAANYLLMGI
                     IYFDKKQYQQSEYYLNLSLLLKQQVNDSIDMAKLNRYMGELELQRGNFAKGITLLNKA
                     LNDAQKQKITTDLRDIYFSLSDAERRKGNYKKAYEYFTSYSALKDSVNASQIAKLEMQ
                     YDFEKREKEQELISRQRDAMQQARLERQRMATLLISIALVLSLALAAVVFRFYREKKR
                     TNQILILQNAEILRQKNEIENQKQEIEAQRDLAERQRDQIAEQQKQITDSIKYASRIQ
                     AAVLPRSQQISTLLRDHFVLYQPKNIVSGDFFWVTQLTDGRIAIAAADCTGHGVPGAF
                     MSMLGITLLKELTATQHFENAADILLRLRRLVIASLNPAETDNDSHDGMDMGMLIVDR
                     KRMEAHYAGAYFPLLLIRDSSVQAPPTHSNPVIADGSTLYEIKGDKMPIGHHIIEERM
                     FTNHTFAIFENDLFYLLSDGYSDQFGGPKNSKFMMVNLKKMLLDIHHLPLKTQQSILQ
                     EKFDEFKGNEKQVDDILIMGFRI"
     gene            4065..>4348
                     /locus_tag="PLW22_11280"
     CDS             4065..>4348
                     /locus_tag="PLW22_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75325.1"
                     /translation="MQSTQFAHRVLKLFAIVLLLQFPFSVFSQPKSDSIAVKALIDSA
                     ANAKSPQVRIAFAREAYHKAIATNSKRLLADATHAMGNALFRLNSYDTAI"
BASE COUNT         1281 a          954 c          888 g         1225 t
ORIGIN      
        1 cgggctttaa ttaagctccg ctgcatctcg gttaatgctt ttcctagctt gtccttctca
       61 ctgagcaact tgaactcggt atcgagtttt ccctcaccaa tctcgcgggc aaattgctca
      121 gtatagttta atccgtcaat tagcttgtta gccgaacgcg acatctcctc aagctcatcg
      181 ccagtatgca ggtatatttt tttattcaca tcaatatcgc cttgggctat actgttaagt
      241 aactcagtag tacgctttat aggttttgaa attcgagctg agatatacca aatggcaaaa
      301 gcttgtagga taatgccgag aataaaaaca agaattgtgg agaacactga ccggcgagcg
      361 tctttaacga tttccgacat gggaatggaa agtataagcc cccatgagtt gggcgattca
      421 cccggctgca atatgctaag tatgttgagg tattccattc cggaattgta ggagaaaaag
      481 aaatggtttt gcttatcctt taccttttgg gtaagcgcaa acttcgactc aagttcaggg
      541 taaatattag caaagtattc ctttgcgttt tcagggtttg aatcggcaat tatacgacca
      601 tctccagcaa tcaggctaac atttgtgccc ggatagggtt ttattttttc aatatcggtt
      661 tggaacttct ccagcgagat atcaacgccg ccaaggccag caaatttacc ttttaccctt
      721 accggtacac taaggctggt tgccagcact tctttaccac catactcaaa ggtgtatggg
      781 tcgagcaaca tctcacagtt gctggttttt gatgcgtagt agttgctggt tacaatatca
      841 cctgtaatat ttttatgctc aatatcaatg cctacctgtc cgttgctaag gtacgctgtt
      901 actgagcgtc ggccgtaatc ttttgtataa cctggacgga acgcagagta ttcaattgaa
      961 taccataccg taaggtatcg gggattgttt gaaacctgat ttcgcagaat attgaaaaag
     1021 atggaatcga gggtaacagt atcgaatttg gggtagatgt aaagggcatg agccagggct
     1081 cttgaaaaac ccatatccaa atcgagttcc gattttaatc ggaaagccga tttctgggct
     1141 tcggcctgtg caatcttaat ggcattatcg cgagcaatac tgttcaccct tatgctaatg
     1201 aatgagccta ttacaatcat tattgctgcc gaggtggtta aaacccaaag caaaagcttg
     1261 gcacctattc gcatctttat tttgtcggca ctaatgttca attttccaat tctcatgttg
     1321 cctagtatgt tttacgatta caattttaat ggtaaaaccg cacaatcgtt tgcaatggtt
     1381 aaaaatacga aaatgtttta gcactataca attttttcaa aatttcgtaa tagttcatca
     1441 gtaaaaatta gcccatcaaa ttttttttgg taaattgctc cttgtagaac aggacagcac
     1501 tatgagaata ctgaatatta aaaagtgcat cacttttttg gtgtttttgg cagttagtat
     1561 ttttgcgggt ttacatgcac aggaaatcga tagcctttta agtctatcag agcattaccg
     1621 gacagttaac cccgattcgg ccgttagcgt tgctaggcaa gctttggcaa aggtcgatgc
     1681 agtaaatcaa cccgatattg cggcccgggt ttactttagt ttagggtatg cctactacaa
     1741 tcgcaacgac tttgagcttg ctcttgaaca tttcaataaa tcgttgatgc ttgctgagca
     1801 atcggataat gttcagctta aagcgttact acacaaccgt attggtaacg tttatcagct
     1861 aaaaagttac tacagccaag ccattgatga gtataaaaaa gcgctggact acaactacaa
     1921 gataggaagc aaggtagaaa ttgcccgcac attggttaac attggaacgg tgtttgcaat
     1981 tacgggtaat ttctcaattg ccttgcagta catgctcgat gcccttgaaa tttttgagca
     2041 atcgggcgaa actgagggta tggcctggac atcgttaagc atttcgcgcc tttttagccg
     2101 attggagatt aacgaaaggg ctttgcagta tgccgaaaat gccttgaacc attaccgcag
     2161 gcttaacaat caaaccggca taaccctggc acaaaccgag ttggccaata tttactacca
     2221 taccggtatg cacgacaagg cgctcgattt actgggtaag gtaattgatg agaattcacg
     2281 cacccgaaac atacacgcac aggcagctaa ctacctgctg atgggaatta tctactttga
     2341 taaaaaacag taccagcaat ccgaatacta cctaaaccta tcgctattgc tgaaacagca
     2401 ggtaaacgat agtattgata tggcaaagct taaccgatac atgggtgaac ttgagctgca
     2461 aaggggaaac tttgccaaag ggattaccct tctgaataaa gcgttaaacg atgcccaaaa
     2521 gcaaaagatt actaccgatt tacgcgatat atactttagc ttatcggacg ccgaacgcag
     2581 gaaaggtaat tacaaaaagg catacgagta ctttaccagc tattcggcac ttaaagatag
     2641 cgttaacgcc agccaaatag ccaagctgga gatgcagtac gattttgaga aaagggaaaa
     2701 ggaacaggag ttaatctcgc gccagcgcga tgccatgcag caagccaggc ttgaacggca
     2761 gcgaatggca accctgctta tttcaatagc tttagttttg agccttgctc tggcagctgt
     2821 agtattcaga ttctaccgcg aaaaaaagag aaccaaccaa attctcatcc tgcagaacgc
     2881 tgagatatta aggcaaaaga acgaaattga aaaccagaaa caggagattg aggcacagcg
     2941 cgatttggcc gaaaggcaac gtgaccaaat agccgaacaa caaaagcaaa taaccgatag
     3001 tattaagtat gcgagccgta ttcaggctgc tgttcttccg cgtagccagc aaatctccac
     3061 actgctgcgc gatcactttg tgctctacca acccaaaaac attgttagtg gcgatttctt
     3121 ttgggtaaca cagctaaccg atggtagaat tgcgatagca gcagccgatt gcactggcca
     3181 cggggttccc ggagccttta tgagtatgct tggcattacc ctgttaaagg aactaaccgc
     3241 aacacaacat tttgagaatg ccgccgatat ccttttgcgc ctccgccgcc ttgttattgc
     3301 ctcgctcaac ccagccgaaa ccgataacga ttcacacgac ggtatggata tgggtatgct
     3361 catagttgac cgaaaaagga tggaggccca ttacgcaggc gcttatttcc ctttgctact
     3421 cataagggat tcctccgttc aggcccctcc aacgcactca aaccctgtta tagctgacgg
     3481 aagtactctt tacgaaatta aaggcgataa aatgcctatt gggcatcaca ttattgagga
     3541 acgaatgttt actaaccaca cctttgctat ctttgaaaac gacctgttct acctgctttc
     3601 cgacggatac tccgaccagt ttggtggccc aaaaaactca aaatttatga tggtgaacct
     3661 caaaaaaatg ctcctcgata ttcaccacct gccattaaaa acccagcaaa gtattctaca
     3721 ggaaaaattc gatgagttta agggtaacga gaaacaggtt gatgatatac ttattatggg
     3781 atttaggatt tgacagccaa gtttcactct atattattaa atctcaaacc tacactcggg
     3841 tttacctgtt cacttttcac tattcctcta actccctcga actccatcaa aatactttct
     3901 atcccgactc aatccgattc attcctctag aaattatcct gcttgaattt tttaattcat
     3961 agtacaagga tttaatcaca ctttaaccat tagcagaaaa tcgtttttaa cttttttttc
     4021 aatttttcgt aatgtatcat atgtttgtat gataaagtct aaccatgcaa agcacacaat
     4081 ttgcacatag ggttttgaaa ctttttgcta ttgttttgct gttgcaattc ccattctcag
     4141 ttttttccca gccaaaatcc gatagtattg cagttaaggc cttaatcgat tctgcggcca
     4201 atgctaaatc gccacaagta agaatagcat ttgcacgcga ggcataccat aaggcaatag
     4261 ccacaaatag caagcgcttg ctcgctgatg ctactcacgc aatgggtaac gctcttttta
     4321 ggctgaacag ctacgatacc gccatcga
//
LOCUS       DAOOWJ010000161         4310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_20472_length_4310_cov_6.394595, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000161 DAOOWJ010000000
VERSION     DAOOWJ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20472_length_4310_cov_6.394595"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLW22_11285"
     CDS             <1..759
                     /locus_tag="PLW22_11285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU75326.1"
                     /translation="KSHFISNITNEIINPFTSIIGLSKAILSVDKENWKKVISMVALI
                     HSEAFNLDFQLRNIFVAAKIEAGEIQPEIMQVDVNNLLESVIESFKYELRKKNLKVEY
                     SFNTSTNLVNPKIFKTDPEKLKLIIANLLSNAVNFSFEGGIIEIITSRGVNKLTICVR
                     DHGIGISQENHKIIFDRFKRLDTGINSLNRGHGLGLSVNRAIIDMLNGSIEIESELGK
                     GACFTISIPEPNVDVDDLATDANEMFFDQDAEIF"
     gene            769..1278
                     /locus_tag="PLW22_11290"
     CDS             769..1278
                     /locus_tag="PLW22_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOU75327.1"
                     /translation="MDHFKSHYLYPASLFADKEPHVVTTILGSCVAVCLYDSVLNQGG
                     INHYMLPLWNGQGLASPRYGNIAIERLIERMISLGSKKENLKAKVFGGGEVLETNLDN
                     FHIGQRNIAIALELLAEHGIPIIAKSVGGNQGRKIEYNTATGEVRHKFIQKTLNPNEV
                     KSISTKKTE"
     gene            1332..2417
                     /locus_tag="PLW22_11295"
     CDS             1332..2417
                     /locus_tag="PLW22_11295"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOU75328.1"
                     /translation="MLNRIKVLIVDDSALVRQTLMEIISSDSELEVIGTAADPYFAAR
                     KMAEVTPDVITLDIEMPRMDGLTFLRKIMTQHPIPVVIISSLTSRATEMGMRALELGA
                     VELITKPQVNTKAFLEESKVIICDAIKAAARSRIARRKILTPPIVEPKLSADVIIPKQ
                     VVTSTPLTTTEKIIAVGASTGGTEAIATFLQAMPVDCPGIVIVQHMPEKFTTSFANRL
                     NDICKITVKEAQNGDAVLPGQALIAPGNYHMLLKRSGARYFVEVVQGPLVNRHRPSVD
                     VLFRSVARFAGQNAIGVIMTGMGDDGAKGLLEMKEAGAYTIAQDEKSCVVYGMPREAV
                     KLDAASTILPLQDIANHIIQRVKSRLS"
     assembly_gap    2523..2622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2623..>4310
                     /locus_tag="PLW22_11300"
     CDS             <2623..>4310
                     /locus_tag="PLW22_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU75329.1"
                     /translation="LQENISRERKLYRFVEKLREGDVEAEYTPDDTDALGKAIVSLRN
                     DIRQSRLEEEKRKKEDEQRRWAAEGMALFGEILRQNNDNIEELTFQVIRNLVNYVGAN
                     QAAFYLLEDDDESDIHFRMTACYAYERRKYADRVIPWGDGLVGACALEKEKIILKKVP
                     DGYLSITSGLGKANPKFLMLIPLKMNDVVHGVIELASFNPFEKYQEEFIDKVSESVAT
                     TIATVKINIRTAKLLKESREQAEELALKEEQMRQNMEELQATQEEASRQSAKFISFSN
                     SVNHTMIRADFDVDGTLLYANTKFLQKLEYSSNSEVEGKHISLFINKKDRTWFDDIWS
                     TLSKGGKHFEGDMKLLTRNGNDLWTIATFTCVRNEAGGVDKILFLGIDTTDQKKLSLD
                     YEGQISALNRSSIKAEFSPTGDLTEANEKFLLTLSYNLNAAKEKSIFEFVPSSERKNI
                     ERVWDDVIHGIPYEGQFRMLTSNGDERWFRCTFTAVNDMYEEVAKVILIANDITREKN
                     MEFETQRQTEQLKKQEEQLRQNEVELNKKLREAKEEVRNQFKEIEKVKIRNEKTL"
BASE COUNT         1329 a          812 c          967 g         1102 t
ORIGIN      
        1 aaatcgcact ttatttcaaa catcaccaat gagattatta acccctttac atcaataata
       61 ggactttcca aggcaatact ctcagttgat aaggagaact ggaaaaaggt aatctccatg
      121 gtggccctaa ttcattccga agcattcaac ctcgattttc agctccggaa tatctttgtg
      181 gctgccaaga ttgaagctgg cgaaatacaa cccgagatca tgcaggttga tgttaacaac
      241 ctgctcgaaa gtgttattga gtcgttcaag tatgaacttc gcaagaagaa cctaaaggtt
      301 gagtactcct ttaataccag caccaatttg gttaatccta aaatttttaa aactgatcct
      361 gaaaagctca agctgatcat agcaaatttg ctcagcaatg cagtaaactt tagctttgag
      421 ggtggcataa ttgaaataat taccagtaga ggcgttaaca agctaacgat ttgtgttagg
      481 gatcatggca ttggtatctc tcaggaaaac cacaaaatta tttttgatag gtttaaaagg
      541 ctcgacacag gaataaattc attgaaccga gggcatggtt taggactatc ggtaaatagg
      601 gctattattg atatgctcaa tggtagcatt gaaattgaga gcgaacttgg caaaggcgct
      661 tgcttcacaa tttcaatacc cgagcccaat gttgacgttg acgatttagc caccgatgct
      721 aacgagatgt tttttgatca ggatgcagaa attttttaaa gccggttgat ggaccacttt
      781 aagtcacatt acctataccc tgcatcgcta tttgccgata aggagcctca tgttgttact
      841 acaattctgg gctcatgtgt ggcagtatgc ctatacgatt cggtattaaa tcaaggtgga
      901 attaatcatt acatgttgcc attgtggaat ggacaggggc ttgcaagccc aaggtatggt
      961 aacattgcca ttgaacgatt gattgaaaga atgatttcac ttggctcaaa aaaggaaaac
     1021 cttaaggcca aggtatttgg gggaggcgag gtgcttgaaa caaatctgga caacttccat
     1081 atcggtcaac gtaacattgc tattgcttta gaactattgg ctgaacatgg aataccaata
     1141 atagctaaaa gtgtaggtgg taaccaggga agaaagattg aatataatac tgctacagga
     1201 gaggttcgcc ataagtttat tcaaaagaca ttaaacccca acgaggtaaa aagtattagt
     1261 acaaaaaaga ccgagtaaat tgcagaatat ttttattgtc cttaatttta aagttgatgt
     1321 aaatggctga tatgctgaat agaataaaag tgctaattgt tgacgattct gcattagtga
     1381 ggcaaacgtt gatggagatc atctcatccg attcagagct ggaggttatt ggaactgctg
     1441 ccgacccata ctttgcagcc cgtaagatgg ctgaggtgac ccccgatgta ataaccctcg
     1501 atattgaaat gccccggatg gatgggctaa cctttcttcg taaaataatg acacaacacc
     1561 ccataccggt tgtaataatt tcgagcctca caagcagggc aactgaaatg ggtatgcgtg
     1621 cccttgaact tggagccgtt gaattgatca ctaaacccca ggttaacacc aaagcttttc
     1681 tcgaagagtc aaaggttatc atttgcgatg caattaaagc agccgctcgc agtagaatag
     1741 caaggcgtaa aattctcaca cctcctattg ttgagccaaa actatcagcc gatgttatta
     1801 tcccaaaaca ggttgttacc tctacacccc ttacaactac cgagaaaatt atagccgtgg
     1861 gagcctctac aggtggaaca gaggccattg ctacattctt acaagcaatg cctgttgatt
     1921 gccctggaat tgttatagtt cagcatatgc ccgaaaaatt tacaacctcg tttgccaatc
     1981 gcctaaacga tatttgtaag atcacggtta aggaagcaca gaatggcgat gctgttctac
     2041 cgggtcaagc gttgattgca cccggtaatt accatatgct tttaaagcga agcggtgctc
     2101 gatattttgt tgaggtggtc caaggcccac tggttaacag gcataggccc tcggttgatg
     2161 ttcttttccg aagcgttgct cgttttgctg ggcaaaacgc aataggtgta attatgactg
     2221 gcatgggcga cgatggagca aaagggctgc tcgaaatgaa ggaggctggt gcttacacaa
     2281 tagcacagga cgaaaaatcg tgcgtggttt acggcatgcc ccgtgaggcc gtaaaacttg
     2341 atgcagcatc taccatactg cctttacagg atattgcgaa tcatattatc caaagagtaa
     2401 aatcacgatt aagctgataa accagttctt ttataataac attattattt catttgaatt
     2461 cgaatcaata gatttgcata taagttgaaa aaatgcttat ttttaagcat caaaaattga
     2521 gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttacagga aaacataagt
     2641 agggagcgta agctctacag gtttgtggaa aagttacgtg aaggtgatgt tgaagccgaa
     2701 tacacccccg atgataccga tgcacttggg aaggcaatag tcagcttacg aaacgatatt
     2761 cgccagagca ggttagaaga ggagaaaaga aagaaggagg acgaacaacg gcgctgggcc
     2821 gcagagggta tggctctctt tggcgaaatt ctacgtcaga acaacgataa catagaggag
     2881 ttaacctttc aggttattcg caatttagta aattacgttg gtgcaaatca ggccgccttt
     2941 taccttttag aggatgacga tgagtccgat atccatttcc gaatgaccgc ctgttacgcc
     3001 tatgagcgta gaaagtatgc cgatagagtt ataccctggg gcgatggtct tgtgggggca
     3061 tgtgctctgg aaaaggaaaa aatcatcctg aaaaaagtac ctgatgggta tttgagtatc
     3121 acaagcggcc ttggtaaggc taacccaaaa tttttaatgc ttatccccct taaaatgaac
     3181 gatgtggttc atggcgtcat tgaactagca tcgtttaatc catttgaaaa gtaccaagag
     3241 gagtttatcg ataaggtttc tgagagcgtg gcaacaacta ttgctactgt taaaataaat
     3301 atacgaaccg caaagctttt aaaggaatcg cgtgagcagg ccgaggagtt agcgcttaag
     3361 gaggaacaga tgcgccagaa catggaagaa ctccaggcta cacaggaaga agctagccgc
     3421 cagtcggcaa agtttatcag cttctccaac tccgtaaacc acaccatgat tcgtgccgat
     3481 ttcgatgtgg acggaacatt gctgtatgca aacaccaagt ttttacagaa attggagtat
     3541 tcctcaaaca gtgaggttga aggtaagcat atctccctgt ttataaacaa gaaggatagg
     3601 acgtggttcg acgatatctg gagtacgcta agtaagggtg gtaaacattt tgagggagat
     3661 atgaagttgc ttacccgcaa tggcaacgac ctttggacaa ttgctacatt cacctgtgta
     3721 cgaaatgagg ctggaggtgt agataaaatc ctattccttg gaattgacac aactgaccaa
     3781 aagaagctga gccttgacta cgaagggcaa attagtgcac ttaaccgttc atcaattaaa
     3841 gcagaattct cgcctacagg cgatttgacc gaagctaatg agaagttcct gctcaccctt
     3901 tcatataacc ttaatgctgc taaggaaaaa tcaatttttg agtttgtacc ttcatcggaa
     3961 cggaagaata ttgagcgcgt atgggacgat gtgatacatg gaattccata tgagggacaa
     4021 ttccgaatgc ttactagcaa tggcgatgag cgttggttcc gttgtacctt cactgctgtt
     4081 aacgatatgt acgaagaggt ggcaaaggtt atcctaattg ccaacgacat tacccgtgag
     4141 aaaaacatgg agttcgaaac tcagcgtcaa acggaacagc taaagaaaca ggaagagcaa
     4201 ttgcgccaga atgaagttga gcttaataaa aagctacggg aggctaagga ggaggtgcgc
     4261 aaccaattta aagaaattga gaaggtaaaa atacggaacg agaaaaccct
//
LOCUS       DAOOWJ010000162         4191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_21280_length_4191_cov_4.181093, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000162 DAOOWJ010000000
VERSION     DAOOWJ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21280_length_4191_cov_4.181093"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PLW22_11305"
     CDS             complement(<1..1172)
                     /locus_tag="PLW22_11305"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU75330.1"
                     /translation="MACTKRQKADLIVTNATVYTVDSAFTVVQSFAVSNGKIVAVGTN
                     NEILSAYSADTILDLTDKFVYPGFIDAHCHFLGYGLNLPKAWLAGAKSWNEVVNRLVE
                     HQNKYPTEWVEGRGWNQNEWEIKEFPTNELLNKAFPNKPVFIVRIDGHAAIANRKALE
                     LAGVTASTKISGGEIVVKNGIPTGVLIDNAMELIHKHIPTPSKEQKAQALLNAQQNCF
                     AVGLTGVHDAGLDADEVELIDSLQQAGNLKMRINAMLNPTHDNFEKYISKGIYSTAQL
                     TVRSIKIYADGALGSRGALLLKPYTDDPKTRGIQVEPTEKLDSVCSLAYKHGYQVCTH
                     CIGDAAVRLMLDTYSKYLPKGNDLRWRIEHSQVVDPADLPRYGEFDVIPSVQTTHAT"
     gene            1281..1790
                     /locus_tag="PLW22_11310"
     CDS             1281..1790
                     /locus_tag="PLW22_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75331.1"
                     /translation="MFKKVLNRFLVIILSLLALWSCENDNFSSSPSIKLTFSTDTVLF
                     DTVFTTIGSSTRYLKVYNRSKSDVRISSVSLAGGSTSAYRINVDGRPGPLVNDIPLRS
                     GDSLFVMVEVTVNPTLGDAPLLIPDSIVFVTNTNVQDVKLVAWGQDVYMHNGVTIETD
                     SVFPVNKPI"
     gene            1808..2722
                     /locus_tag="PLW22_11315"
     CDS             1808..2722
                     /locus_tag="PLW22_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75332.1"
                     /translation="MDKNATLTIPAGSRLHFHNNAQLVVAGTLKVEGSASLPVTMQGD
                     RLEKFYSDKGGQWGGVWLSAGSRNNSIAWADIRNAIIGLMVDTCFTPGVPTLVLSHSK
                     IGNSSYVNLLARGAKLEVDNCLFYNAAYSSVALTLGGTYRFYHTTISNYWGDYMFRKG
                     PALLLNNYYTYQLVPGGPVLVEPRDLNEASFFNSIIYGSISNELGLDYKYNGQPVNAN
                     FNYSFSDCILRVPSTYNLNDPEHFTRVIREDPRFKDVAKWNFELDTLSPAKDIGDITI
                     ATGFPVDLKGVNRLADGKPDLGAFERVE"
     gene            2734..3798
                     /locus_tag="PLW22_11320"
     CDS             2734..3798
                     /locus_tag="PLW22_11320"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOU75333.1"
                     /translation="MKRSLQHRVFCLFRLVFLVCLAGLPIIMLAQNQAASRHRVMFYN
                     VENLFDPFDDSLTRDDEFTPTGQRYWTWNKMETKINGIYKVIMAVGELDPPVLVGLCE
                     VENGFVLHKLVNDTPLSKFEYRVVHRESPDPRGIDVALLYRPDRFKVQEREFVPVIFP
                     DDSARKTREILYVSGMLGSDTLHVFVNHWPSKYGGELESESGRFAAASTLKRKIDSIR
                     VFYPQARILVMGDLNDEPESAPVVEGLGACERNNPNCPSGLINIAAILKSRGFGSYKY
                     QGTWGIIDQIIVSQSLLSGKHRLYTTPQKAAVFESDQLLEPDKQYSGNRPFRTFIGYR
                     YHGGFSDHLPVYIDLLENEP"
     gene            3944..>4191
                     /locus_tag="PLW22_11325"
     CDS             3944..>4191
                     /locus_tag="PLW22_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75334.1"
                     /translation="MLGMTIPLFPYSHCHAELAEASLCFFNSINRIPHPKRNAKSSGI
                     KARPENGSGVERGSGLYKRYYMSHAVGVANSIAWQFHPR"
BASE COUNT         1031 a          953 c          986 g         1221 t
ORIGIN      
        1 gtggcatggg tggtttgaac cgatgggatt acatcaaact cgccataacg cggcaaatcg
       61 gctggatcaa ccacctgtga gtgctcaatg cgccaccgta ggtcattgcc tttgggtagg
      121 tacttgctat aagtatcaag catcagccta acggctgcat cgccaatgca gtgcgtgcat
      181 acctggtagc catgcttgta tgccaagctg caaaccgaat caagtttttc agttggttcc
      241 acctgaatgc cgcgggtttt aggatcgtcg gtgtagggtt tgagtagcag tgcgccacgt
      301 gaacccaatg cgccatcggc ataaattttt atcgacctaa ccgtcaattg cgctgtgcta
      361 tatataccct tgctgatgta cttttcaaaa ttgtcatggg tagggttaag catggcatta
      421 atgcgcattt taaggtttcc tgcttgctgc aacgagtcga taagttcaac ctcatcggca
      481 tcgaggccgg catcgtgaac gccggttagc ccaacggcaa aacagttttg ctgagcattt
      541 agcagagctt gcgctttttg ttccttactt ggtgtgggta tgtgcttgtg tatcagttcc
      601 atggcattat caatgagaac cccggtgggt atgccatttt taactactat ttcgccaccc
      661 gatatttttg tgctggcggt tacgccagct aattcaagcg ctttcctgtt ggcaatggcg
      721 gcatggccat caatcctaac tataaaaact ggtttgtttg gaaatgcttt gttgagtaac
      781 tcgttggttg gaaactcttt gatttcccat tcgttctggt tccatcccct gccttcaacc
      841 cattccgttg ggtatttgtt ttggtgctca accagacggt taaccacctc gttccacgat
      901 tttgcccccg caagccatgc cttgggtagg tttagcccgt accctaaaaa gtgacagtgg
      961 gcatcaataa atccggggta tacaaattta tctgttagat ccaatatggt gtcggcagag
     1021 taagctgaga gtatctcatt gtttgttccc acggctacta ttttgccatt gctaacggca
     1081 aagctttgaa ctactgtgaa tgctgagtca accgtgtaaa ccgttgcatt ggtaacaatt
     1141 aaatcggctt tttgccgttt agtgcatgcc ataacagcta tacttaaagt taaaaatatt
     1201 gataatgagc ttgtcttcat ttccgttaag ctatctttgt tgtgagtttt taccaaaagt
     1261 agaaaattat ttttttgttg atgtttaaaa aggtattaaa tcggttttta gttatcattc
     1321 tgtcattgtt ggcgctgtgg tcgtgtgaaa acgataactt ttcgtccagc ccatcaataa
     1381 agttaacctt tagcaccgat acggtgctgt tcgataccgt gtttaccacc attggttcat
     1441 caacccgtta cctaaaggtt tacaacagga gtaaaagcga tgtgcggatt tcgtcagtat
     1501 cgttggctgg tggcagcacg tcggcataca gaataaatgt tgacggacgc ccaggcccat
     1561 tggtgaacga tattccttta cgttccggcg atagcctttt tgttatggtt gaagtaaccg
     1621 ttaaccctac ccttggcgat gcacccttgc taatacctga ttccattgtt tttgttacca
     1681 atactaacgt ccaggatgtt aagctggttg cctgggggca ggatgtttac atgcataacg
     1741 gagtaaccat tgaaaccgat tcggttttcc ccgttaataa acccatctga tttacgatta
     1801 cctttttgtg gataagaatg ctacccttac cattcctgct ggatcacgat tacacttcca
     1861 taacaacgca cagcttgtag tagcgggaac cctaaaggtt gaaggttcgg ctagcttgcc
     1921 agtaaccatg cagggcgata gactggaaaa gttttactcc gacaagggcg gacaatgggg
     1981 tggggtgtgg ttgagtgcag gttcccggaa taactccatt gcttgggctg atattaggaa
     2041 tgccattatt gggttgatgg ttgatacctg ctttacaccg ggtgtgccaa cccttgtact
     2101 ctcgcactcc aaaataggca attccagtta cgttaacctg cttgcccgtg gggcaaagct
     2161 ggaggttgac aactgccttt tttacaatgc tgcctactca agtgtggctc ttacccttgg
     2221 ggggacttac cggttttacc ataccacaat atctaactac tggggcgact acatgttccg
     2281 gaaagggcct gcgctcctgc tcaacaacta ctatacatac caacttgtcc ctggtggacc
     2341 ggttctggtt gaacctcgcg atttgaacga ggcatcattc tttaattcta ttatttatgg
     2401 tagcatatcc aatgagttag gactggatta taagtataat ggccaaccgg ttaatgctaa
     2461 tttcaattac tctttttcgg actgtatcct tagggtgcct tcaacctaca acctgaatga
     2521 ccctgagcat tttacccggg ttattcgcga agacccccgg ttcaaggatg ttgccaagtg
     2581 gaattttgag ctcgataccc ttagcccagc aaaggacata ggtgacatta ctattgccac
     2641 tggttttcca gtcgacttaa agggtgttaa ccgacttgcc gatggtaaac ccgatttggg
     2701 agcctttgaa cgtgttgagt aaacctctgg agaatgaaac gcagcctaca acaccgagtt
     2761 ttttgcctgt ttaggcttgt ttttttagtc tgtttggcag gcttgcccat tataatgctt
     2821 gctcaaaacc aggccgcatc aaggcatagg gtgatgttct ataatgtcga gaatcttttt
     2881 gacccattcg acgactcgct aacccgcgac gatgagttca cccccactgg ccagcgctat
     2941 tggacatgga acaagatgga gacaaaaatc aatggcattt acaaggtgat aatggccgtt
     3001 ggtgaactcg atcctcctgt gctggttggc ctttgcgagg ttgaaaatgg ttttgtgctc
     3061 cacaaacttg taaacgatac cccactcagc aagtttgagt acagagtggt tcaccgtgag
     3121 tcgcctgacc ctcgcggaat cgacgtggcc ctgctttacc gccccgatag gtttaaggtt
     3181 caggagcgtg agtttgtccc agttatattt cctgatgatt cggcgcgtaa gacgcgtgag
     3241 attctttatg taagcggtat gctgggaagc gatactctac atgtttttgt aaaccattgg
     3301 ccatccaagt atggtggcga gctggaatcg gaaagcggca ggtttgccgc tgcaagtact
     3361 ctcaagcgca agatcgactc aataagggtt ttctaccctc aagcccgaat actagtaatg
     3421 ggcgacctga acgatgagcc agagagcgcc ccggtggttg aaggcttggg agcatgtgaa
     3481 cggaacaacc ccaactgccc atcggggtta attaacattg ctgctattct taaaagccgt
     3541 ggttttggaa gttataagta tcagggtaca tggggaatta ttgatcaaat tattgtgtcg
     3601 caaagccttt taagcggaaa acataggtta tacaccacgc cgcaaaaggc agcggttttt
     3661 gaaagcgatc agctgcttga gcccgataag cagtacagtg gtaataggcc tttccgaaca
     3721 ttcattgggt accgatacca tggcggcttc agcgatcacc tcccggttta tatcgacctt
     3781 ctcgagaatg agccataaga aaagcacgcc agtgctgcgt gcttcctctc ttttttaacc
     3841 tagataaatc aatagaaaaa atctgttaat acattgtact tgggtttaat tgttcacttt
     3901 tcacttttcc tcaaactttt cctggcaaaa gagattcctc gatatgctcg gaatgacaat
     3961 cccccttttt ccttactccc attgtcatgc tgagctcgcc gaagcatccc tttgtttctt
     4021 caactctatt aataggattc ctcatccaaa aagaaatgct aaaagcagtg gcataaaagc
     4081 cagacccgag aatgggtcgg gggtggaacg tggtagtggg ctttataagc gatactacat
     4141 gagtcacgcc gtaggcgtgg cgaatagtat cgcgtggcag ttccacccac g
//
LOCUS       DAOOWJ010000163         4164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_21438_length_4164_cov_5.125335, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000163 DAOOWJ010000000
VERSION     DAOOWJ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21438_length_4164_cov_5.125335"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLW22_11330"
     CDS             <1..227
                     /locus_tag="PLW22_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOU75335.1"
                     /translation="FKFLKGIHLNDTKKGLGSRVDRHENIGDGLLGIEPFRLIMADPR
                     FNGIPIILETPDETRWADEIMMLKKFANAL"
     gene            269..535
                     /locus_tag="PLW22_11335"
     CDS             269..535
                     /locus_tag="PLW22_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75336.1"
                     /translation="MAYIKNATWIYKPFTQISEDALVNELKQLQVMEIDAGMEIPMHG
                     NIASHIPLGEEGAVRVFGRDDSGHEADLYEVKPGVACMFHLHRL"
     gene            532..747
                     /locus_tag="PLW22_11340"
     CDS             532..747
                     /locus_tag="PLW22_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75337.1"
                     /translation="MIDTPAITYAKAKQKSKFIPVPKELSNLWFVKYPSWRHYIIGLY
                     QNRLDELIRQNERVVAQNEMIEIQKIR"
     gene            789..1160
                     /locus_tag="PLW22_11345"
     CDS             789..1160
                     /locus_tag="PLW22_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75338.1"
                     /translation="MPVEGQLKEFFADYFTRYIPKDIVSGDFYWTYYLNNKVLYADAT
                     GHRCLDLLSILGISLMNEICNNFSGNASDSLNTMRYKTKMALKQTSFDSGPRDGFDIQ
                     LCIFHTRQGIVSMPAQTITFC"
     gene            1154..1369
                     /locus_tag="PLW22_11350"
     CDS             1154..1369
                     /locus_tag="PLW22_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75339.1"
                     /translation="MLSRNNQIFEFNADKMPVGVAFKEEEPFTNHSIRVQDNDIFYLF
                     SDGYKNQFGGPEQQNFSRKGFTTCLAR"
     gene            complement(1836..3710)
                     /locus_tag="PLW22_11355"
     CDS             complement(1836..3710)
                     /locus_tag="PLW22_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOU75340.1"
                     /translation="MKRIAGILMLVLQVLPYTISAQKAVEVNSNITDVVVYLDAASIT
                     RKAQVNLPEGRTKISFTGLSPFIDFKTISITTPEGVSIESINHSNNYLSKKDKNEEAE
                     MLTKKLSDLEAQQKVISTSIAVVNEEINFLNDNRSISGKNEAVSVQTLKDASTFYGSR
                     IKELRFKSLELSEQHNDITKKIEETRRQLSVISSKPSTPTGEIEVLLETNSPKNEVPI
                     VVNYLVKNAGWFPRYDIKANVLDRPITLVYKANIRQETKEDWKNVKVKLTNSEPNVSG
                     VAPALKPYLLNYGLQPPRYGKIVNVVQGRVTDTHGNPLPGTNIVISGTSIGTIANTEG
                     KYSINIPTDANSLTFSMVGFKPRTLPINSNTINVVMEEDEVRLEEVTVVAYGVASDDY
                     DRSRKSEVRKEAPKPKITGGAVTESGILEKLTSVEFELAKPFSLESENKSQDVKLLTY
                     EIPAEFKYSTTPKVDKGAYLWVNIPAWEMYNLLDGEANVFFENTYTGSTLIDTRKALD
                     TLQLSLGVDKSISVKREKVKEFTKKQLIGSSKEETRSWKITIKNNKSQDISITVLDQI
                     PVSINEEIKVNVLEKSNAAINPENGEVKWDLLIPAGQFRELILTYSVRYPKNRNLYVE
                     "
     gene            complement(3821..>4164)
                     /locus_tag="PLW22_11360"
     CDS             complement(3821..>4164)
                     /locus_tag="PLW22_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOU75341.1"
                     /translation="KSGIEYMMFLADWYTNNAGTEGIGFFQIGGGIAGDFPICVVPML
                     HQDLGRTGVPFWSYFCQISDSTTSYGSYSGAVPNEKITWGKLNIDTPKFIIESDATIV
                     APLIFAYVLGW"
BASE COUNT         1189 a          870 c          825 g         1280 t
ORIGIN      
        1 gttttaaatt ccttaagggc attcacctta acgacaccaa aaaagggcta ggcagtcgtg
       61 tagatcgcca tgagaacatt ggcgatggcc ttttgggaat tgagcctttc cggctcatta
      121 tggctgaccc acgctttaat ggaatcccca taatccttga aacacccgat gaaacccgct
      181 gggcggatga aattatgatg ctaaagaaat ttgcaaatgc tttgtagcaa ttatcaagct
      241 aaaaaactat ttatctccca ataagttaat ggcttacata aaaaatgcga catggatata
      301 caaacccttt acccagattt cagaggatgc gctggttaac gagcttaagc agctgcaggt
      361 aatggaaata gatgccggaa tggagatacc catgcatggg aatattgcct cgcacattcc
      421 tttgggagaa gaaggagcgg ttcgtgtttt tggacgcgac gattcagggc atgaagccga
      481 tttatacgag gttaaaccgg gagtagcctg tatgtttcat ttacatcggc tatgattgac
      541 actcctgcta taacctatgc caaggcaaaa caaaaatcaa aattcattcc tgttcctaaa
      601 gaactgtcaa acttatggtt tgttaagtac ccgtcgtggc ggcattacat tattgggtta
      661 taccaaaacc gcctcgatga acttatcagg cagaatgaaa gggttgtggc acaaaacgaa
      721 atgattgaaa ttcaaaaaat cagataatcc agagcattaa ctatgcccga aacattcaga
      781 aagcggtttt gccagttgag ggtcagctta aggagttttt tgccgactac tttacacgtt
      841 atattcccaa ggatattgtt tccggtgatt tttactggac atactaccta aacaacaagg
      901 tattatatgc cgatgctacc ggacataggt gcctggatct tttgagtata ttaggcatat
      961 cgcttatgaa tgaaatctgc aataacttca gcggtaacgc ctcggactct ttaaacacca
     1021 tgcgatacaa aaccaagatg gcacttaagc aaaccagctt tgactcgggt ccccgcgacg
     1081 ggttcgatat tcagctttgc attttccaca cccgccaagg cattgttagt atgccagcgc
     1141 aaacaataac cttttgctaa gcagaaataa ccagatcttt gagtttaatg ccgataaaat
     1201 gccggttggg gttgccttca aggaagagga accgtttacc aatcactcta taagagtaca
     1261 agataacgat atattttacc ttttttccga tgggtataag aatcaatttg gaggacctga
     1321 acagcaaaac ttttcaagaa aaggttttac aacctgctta gccaggtaag ctcaaaaccg
     1381 cttgccaagc aaaaagaaat tcttgaaaac cattttaacg aatggaaggg cagcaacacg
     1441 caggttgatg atgtactggt tttggggata aaaatcaggg ctaaggacta gcataactta
     1501 gagctatacc attccatgct attacctatt caccgataaa aattcaacca actaagttaa
     1561 taaacattgc cctgaaaatt agatattgaa aactacagat catcaattag tgaaacctgt
     1621 ttttaattga aatcaccttt ttatcataaa tgtttcttta aaataaaaaa acaactaaaa
     1681 ggttctacca atgcttatac caatcggaat aaaggggata tcatcggttt cgcctcctga
     1741 aatcgctttg atattaataa aaaacaccca tggtttatcg aattttttaa taccccatgg
     1801 gtgtttggta tcatgttaaa atatccacaa caaggttact caacatacaa atttctattt
     1861 ttaggatacc ttaccgaata ggttaaaatg agctccctga attgtccagc tggaattaaa
     1921 agatcccact ttacttcacc attttctgga ttgatagctg cgtttgattt ttcaagaaca
     1981 tttactttga tttcttcatt tatggaaaca ggtatctgat ctaaaaccgt tatgcttata
     2041 tcctgacttt tattgttctt aatggttatc ttccagctgc gggtttcctc tttactacta
     2101 ccaattagct gcttcttggt aaactctttt accttttcac gcttaacgga tatggattta
     2161 tcaactccca atgaaagctg tagggtatcg agagctttac gtgtatcaat aagggttgat
     2221 ccggtatagg tattttcaaa gaaaacgttt gcttcgccat cgagcaggtt gtacatttcc
     2281 caagccggta tatttaccca aaggtatgct cccttgtcaa cctttggagt ggttgaatat
     2341 ttaaactcgg caggaatttc gtaggtaagc agctttacgt cctgcgattt attctccgac
     2401 tctaacgaga agggttttgc cagttcaaat tcaacacttg taagcttttc aaggattccc
     2461 gattccgtaa ccgctccacc tgtgatcttt ggctttgggg cttcttttct tacttcgctt
     2521 tttctactcc tatcataatc atcgctggct accccatacg ctacaactgt cacctcctcg
     2581 agccttacct catcctcttc cattactaca ttgatagtat ttgaattgat gggtagtgta
     2641 cggggtttaa agccaaccat gctaaatgtt aaactgttag catcggtcgg gatattgatg
     2701 ctatacttcc cttcggtatt ggcaattgta ccaattgagg tcccgctaat aactatgttg
     2761 gtgcctggca aagggttccc gtgagtatcg gttactcgtc cctgaactac attaacaatt
     2821 tttccgtaac ggggtggttg taaaccgtag ttcaacaggt atggctttaa tgcaggagca
     2881 actcccgaaa cattgggttc agagttggta agcttaacct tcacattttt ccaatcctcc
     2941 ttggtttcct gccttatatt tgccttataa accagggtta ttggacggtc aagaacgttt
     3001 gcctttatat cgtaacgggg aaaccatccg gcattcttta ctaggtagtt aaccacaatg
     3061 ggtacctcat tttttggtga attcgtttct agcaaaacct ctatttcgcc cgtaggagtg
     3121 ctaggtttac tgctaatgac tgaaagttgc cttcgggttt cctcaatttt ttttgtgatg
     3181 tcgttgtgct gttcactaag ctctaggctc ttgaaacgta gttccttgat ccgtgaacca
     3241 taaaaggttg aggcatcttt tagagtttgg accgataccg cttcgttttt tccgcttata
     3301 cttcggttat cgttcaaaaa atttatctcc tcgttaacta ccgcaatact tgtacttatg
     3361 actttctgct gtgcttcaag atcgcttagc ttcttggtta gcatttctgc ctcctcattc
     3421 ttatcctttt tgctcaggta gttatttgaa tggttaatag attcaatgga gaccccttcg
     3481 ggtgtcgtta tgcttatggt tttaaaatcg ataaagggcg aaaggcctgt gaaactgatt
     3541 tttgttcgcc cttcaggaag attaacctgt gcctttcggg ttattgaggc tgcatcgagg
     3601 taaacaacca catcggtgat gtttgaatta acctcaacgg ctttttgtgc tgaaatggta
     3661 taaggtaaaa cctgtagcac taacattaaa atacctgcta ttcgtttcat aaatcatttt
     3721 ttaattcaac gtaagttagc aaattttatt ccaaagagcc aaaaaaatag gccatggatt
     3781 gtccatggcc aatgagtatg aatttgaaag atgctttctg ttaccaaccc agtacgtagg
     3841 caaaaattag cggtgccaca atggtagcat cggactcaat gataaacttt ggggtatcga
     3901 tattgagctt tccccaggtg attttttcgt ttggtaccgc accagagtag gacccataac
     3961 ttgtagttga gtcgctaatt tggcagaagt agctccagaa aggaactccg gtacgcccaa
     4021 gatcctgatg tagcatggga accacacaaa ttgggaaatc gcctgcaata ccaccaccaa
     4081 tctggaagaa accaataccc tcggttccgg cattgttggt gtaccagtcg gcaaggaaca
     4141 tcatatactc aatacccgat ttta
//
LOCUS       DAOOWJ010000164         4118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_21764_length_4118_cov_5.953975, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000164 DAOOWJ010000000
VERSION     DAOOWJ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21764_length_4118_cov_5.953975"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..849
                     /locus_tag="PLW22_11365"
     CDS             388..849
                     /locus_tag="PLW22_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU75342.1"
                     /translation="MEVEKILSKYPRRKDYLLEILHDIQNHSTKNFIPEEAVQRVAEH
                     LNIKKAQVYGVIGYYSMLSIKPRGRYVIRVCASPICNMMGSCSLLDYLKQKLDVELGE
                     TSADGLFTLETSECLGNCAAAPAMMVNQEMYGNLSPGMIDSLLEHYSRQTK"
     gene            861..1427
                     /locus_tag="PLW22_11370"
     CDS             861..1427
                     /locus_tag="PLW22_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75343.1"
                     /translation="MLKEYRIALRNSGLINPESIDEFIAADGYQALRKALKSEPLGIY
                     EEIYDSGLRGRGGAGFPTGAKEKAAAVICSDCEKYVICNADEGEPGTFKDRIIMETDP
                     HTLIEGLVIAAYSIGAHRGFVYIRGEYELSIQRVQNAIEQAYGRGFLGKNILGSGFDF
                     DLEVRRGAGSYLCGEELTLIESVEGKRG"
     gene            1593..2114
                     /locus_tag="PLW22_11375"
     CDS             1593..2114
                     /locus_tag="PLW22_11375"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOU75344.1"
                     /translation="MSGDVNKPGFYEFPMGITLRELIYGVAGGIRNGKPFGAALIGGA
                     AGTFASERQLDVPMGYDKLREVGLTLGSGAVIVMDSTRNLNQTMLSILKFFKHESCGK
                     CIPCRIGTTQLYEALKENLTHQDFKPDTVYPELIRQAEMIARTSLCPLGQSPILPLKS
                     LWEHILVKQTANK"
     gene            2117..>4118
                     /locus_tag="PLW22_11380"
     CDS             2117..>4118
                     /locus_tag="PLW22_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU75345.1"
                     /translation="MAKVKIHIDGKEILANEGANLLQESRLNGFNIPGLCYHHKLTPT
                     GACRLCLVKVEGQRGMVASCTLTVKEGMNIVAFDQELEDTRRFLLDYLVSEQNTHYDG
                     TYTDELRELIHRYNLHKPENRYFKPVKPHVEFKVDDSSPVLTYDPSKCIKCFRCIKAC
                     DEVQGKNVLSFYERGLNSYIVAGTGVWGDSECDGCGECIQLCPTGAIVEKPNREFIKL
                     ERVEQRVRTTCPYCGVGCQIELLVQDGKIVRSNGVEGVSPNDGRLCVKGRFGYEFVHS
                     PDRLTVPLIRKNGELVESSWDEALTLIAQKFGEIKEKYGSDSLAGYASAKCTNEDNYI
                     FQKFIRTVFGTNNIDYCTRLCHASTVTAMLKSIGDGAGSNSIEDFETTDCLVVTGNNI
                     IETHPITATYVKRGAARGMKIIVIDPKWTPLVPYATLWLQPRLGTDVALLNGMVREII
                     ANNWVDHNFIESRIEGGMQAFLELKDLVEKYTPEATEKITGVPALKIKDAARIYGTAK
                     TAMVATGMGMSQQVTGTHNVFSLLNMILITGKIGKERCGIDPPRGQNNVQGATDVGCH
                     PTMYSGYIPITNTENRQKVAEVWGVPFESLSDKPGLTTVEIIQAAYQGKIKGLYIMGE
                     NPMISDPNLNHTEEALKKLDFLVVQDIFLTETARFAHVVLPAS"
BASE COUNT         1155 a          889 c         1015 g         1059 t
ORIGIN      
        1 tgttgattta agacaaatct gtgtttgtat ttgctgtcaa ctaacaacaa tcaaccatca
       61 acgtaatctg tcatcctgag cgtagcgaag aatctctatt tatatatcgt taagtgatgt
      121 ttcggtggag tttatcccgg tatatcggga ttcaacatga aaaaccttct taactaaaag
      181 actatgactt ttaatttcga tttttgcaaa gccagaaata agcagaaaca acacgtcaat
      241 tcattcaaaa acacgaaatc gttaaattga ttccccaaat ttttacatcc cttcattcac
      301 ttccctccca agtagtctaa ttctaaattg caccttactg ttgcttgtag tttctttttg
      361 gcttaactta aggcaaaaat cttgagtatg gaggttgaaa agatactcag caaatatcct
      421 agacgcaagg attacctgct ggaaatcctg cacgatatcc agaaccatag caccaaaaat
      481 ttcattcctg aggaggctgt tcaaagggtt gcagagcatt tgaatatcaa aaaggcacag
      541 gtttatgggg ttattgggta ctactccatg ttaagcatta aaccgcgcgg aaggtatgtt
      601 attcgtgtat gtgcttcgcc catctgcaac atgatgggta gctgtagcct tttggattac
      661 cttaaacaaa aacttgatgt tgagctgggc gaaacctccg ccgatggact ttttaccctt
      721 gaaacatcgg agtgcttagg caactgcgct gcggcaccgg ccatgatggt aaatcaggaa
      781 atgtacggta atctttcgcc gggaatgatc gatagcctgc tggaacacta ctcccggcaa
      841 accaaataaa accgaatgtt atgctcaagg aataccgaat agccttacgg aattcggggc
      901 ttattaaccc tgaaagtatc gatgagttca ttgcagccga tggctaccag gctttgcgta
      961 aggccctgaa aagtgaaccc cttggaattt atgaggagat ttacgattcc ggtttgcgtg
     1021 gccgtggtgg tgcgggtttc cctaccgggg caaaggaaaa agctgctgcc gtaatctgct
     1081 ccgattgcga aaagtatgtg atttgcaacg ccgatgaggg cgagcctggt acctttaagg
     1141 ataggattat catggaaacc gacccgcata cgcttatcga agggttggtt attgctgctt
     1201 acagcattgg tgcgcacagg gggttcgttt atatcagggg tgagtatgag ttaagtatcc
     1261 aaagggttca gaatgctatt gagcaggcct atggccgtgg gtttctgggc aagaatattt
     1321 tgggtagcgg attcgatttc gacctggagg ttcggcgtgg tgcaggctcc tacctttgcg
     1381 gtgaggagct aaccctgatt gagtcagttg aggggaaaag ggggtaaccg cgtattaagc
     1441 ccccttaccc tgctgagaag gggctatggg gcgcaccaac cctaatcaac aacgttgaaa
     1501 cgcttgcaaa ttttcctgcc atcattctga atggagcaaa atggtatcgt tccataggca
     1561 ccgaaaaatc gcccgggaca aaaattttca ccatcagcgg cgatgtgaac aaacccggat
     1621 tttatgagtt cccaatggga ataactctgc gtgagttaat ttacggggtg gcaggtggta
     1681 ttaggaatgg aaaacctttt ggtgcggcat tgattggcgg ggcagcaggc acatttgcct
     1741 cggaacggca attggatgta cccatgggct acgataaact ccgcgaggtt gggctaacac
     1801 ttggatcggg tgcggttatt gttatggata gcacgcgcaa tcttaaccaa accatgctct
     1861 caatccttaa attctttaag catgagagct gcggtaagtg catcccttgc cggataggca
     1921 ctacccaact ttatgaggca ctaaaagaga acttgacaca tcaggatttt aaacccgata
     1981 ctgtttaccc tgaacttatt aggcaagccg aaatgattgc ccgtacatcg ctctgtccgc
     2041 ttgggcaatc acccatactg cccttgaaaa gtttatggga acacattctg gttaaacaaa
     2101 cagcaaataa gtaactatgg ctaaagttaa gatacatatt gatggcaagg agattttggc
     2161 taacgagggt gccaatctac tgcaagagtc aaggctaaat gggttcaata ttcccgggct
     2221 ttgctatcac cacaagttaa ccccaacggg tgcttgccgc ctatgcctgg ttaaggtcga
     2281 aggccaacgg ggtatggtag cctcatgtac cctcaccgtt aaggagggaa tgaacattgt
     2341 tgcctttgac caggagctgg aggatacccg tcgtttcctt ctcgactatt tggtaagcga
     2401 gcaaaacacc cactacgatg gcacttatac cgatgagcta agggaattga ttcataggta
     2461 taatctccat aagccagaaa atagatactt taagccagta aagccccatg tagagtttaa
     2521 ggttgatgat agttcgcccg tgctaaccta cgatccctca aagtgtatca agtgcttcag
     2581 gtgcattaag gcatgcgatg aggtgcaggg taagaatgtg ctgtcgtttt atgaacgtgg
     2641 gttgaactcg tacatagttg ccggaacggg tgtttggggc gacagcgagt gcgatggctg
     2701 tggcgagtgc atccagcttt gcccaaccgg agcaatagtg gaaaaaccca acagggagtt
     2761 catcaaactc gaaagggtag agcaaagggt tcgaactact tgcccctact gcggagtggg
     2821 atgccaaatt gagttgcttg tccaggacgg caaaattgtt cgcagcaatg gcgttgaagg
     2881 cgtttcgcca aacgatggca ggctttgcgt gaagggacgc tttggctatg aatttgtcca
     2941 tagccccgat aggctaactg tgccccttat ccgcaaaaat ggcgaattgg ttgaatcatc
     3001 ctgggacgag gcgctaacct taatagccca aaagtttggc gagattaaag aaaagtatgg
     3061 ctccgactcg ttggccggct atgcctcggc caaatgcacc aacgaggata actacatctt
     3121 tcagaaattc atcagaactg tttttggaac caataatatt gactactgca ccaggctttg
     3181 ccatgcctca acggttactg ccatgctcaa atccattggc gatggggctg gcagcaactc
     3241 aattgaagat tttgaaacca ccgattgttt agtagtaacc ggaaataata ttattgaaac
     3301 ccacccaatt actgcaacct atgttaagcg gggtgcagca agaggaatga aaatcattgt
     3361 gattgatccc aaatggacgc cacttgttcc ctatgcaaca ctatggctgc aaccccgttt
     3421 gggtaccgat gtggctctcc taaatggaat ggttagggaa attatagcca ataactgggt
     3481 tgaccataac tttattgaaa gcaggattga gggtggcatg caggcctttc ttgagcttaa
     3541 agatttagtt gaaaagtata ccccagaggc caccgaaaaa attacagggg ttcctgcact
     3601 gaaaatcaaa gatgcggcac gaatatacgg aactgccaaa acggccatgg ttgcaaccgg
     3661 aatgggtatg agtcagcagg ttactggaac tcataacgtg ttcagcttgc ttaacatgat
     3721 actcattacc ggcaaaatag ggaaggagcg ctgtggaatt gatccaccac gcggtcagaa
     3781 taatgttcag ggcgctaccg atgtgggatg tcatccaacc atgtactccg gatatattcc
     3841 aattactaat accgagaacc ggcagaaagt ggcagaggta tggggcgtgc ctttcgaaag
     3901 cctttccgat aaacccggtt taacaaccgt ggagatcatt caggctgcat atcaagggaa
     3961 aatcaaaggc ctttacatta tgggtgaaaa ccccatgatt agcgatccta acctgaacca
     4021 taccgaggag gccctcaaaa agttagattt tctggtggta caagatattt tccttactga
     4081 gactgcccgt tttgcacatg tggtgctacc agcatcag
//
LOCUS       DAOOWJ010000165         3964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_22920_length_3964_cov_9.156818, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000165 DAOOWJ010000000
VERSION     DAOOWJ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22920_length_3964_cov_9.156818"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2363
                     /gene="leuS"
                     /locus_tag="PLW22_11385"
     CDS             <1..2363
                     /gene="leuS"
                     /locus_tag="PLW22_11385"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOU75346.1"
                     /translation="MFKHWYNNKTQKAEPIENLIAEFEQNGNINVDAACSEVRTFSAS
                     EWKAMSEKEQQEILLAYRLAYLADVMVNWCPALGTVLANDEVKEGVSIRGGHPVEQRK
                     MRQWCLRISAYAERLLNDLDQLDWTDSLKDIQRNWIGKSEGAEVWFRIDGTDRKILIF
                     TTRPDTIYGATFMVLAPESELVDELTTPEYAEKMEQYRQEVKRKTERERMAEARKVTG
                     QFTGSYAINPFTNERIPIWVSEYVLAGYGTGAIMAVPAHDSRDFAFAKTFGLPIIQTV
                     IRPGEQPTNPESWTESYDSKEGVVINSPLINGLEVKDAISKICEVIESRGLGKRKVNY
                     RLRDAIFSRQRYWGEPFPIFYKDGMPYPLPEEKLPLELPEIDAYLPTEKGEPPLGRAK
                     NWHTPEGYPYELSTMPGFAGSSAYYLRYMDPHNDNALVSAEANKYWENVDLYIGGTEH
                     AVGHLIYSRYWNKFLYDLGYVCKQEPFKKLINQGMIQGRSNFVYRVKGTNQFVSHNLK
                     DKYDVTPIHVDVNIVSNDILDIEAFRKWRPEFENAEFILEDGKYVCGWAVEKMSKSMW
                     NVVNPDTIVERYGADTLRMYEMFLGPLEQSKPWDTNGIDGVHKFLRKFWRLFHNNANE
                     FEVSANEPTAQELKVLHKTIKKVTEDIEKFSFNTGVSAFMICVNELSDLKCNKRAILE
                     PLTVLIAPYAPHIAEELWHLLGHETSVNTAAWPKFNEEYIKESTFTCPISFNGKTRFT
                     LELPLNLKADEVEKIVLADENTLKYLDGKQPKKVIVVPNKIVNIVV"
     gene            2464..2883
                     /locus_tag="PLW22_11390"
     CDS             2464..2883
                     /locus_tag="PLW22_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU75347.1"
                     /translation="MDKSDNKTKSFKDLVVWQKAHAFVLNIYQQVKIFPDDYQAIVGD
                     MLCESATAIATNIVGGYKKKDREDKLLFLTTAQEALEECRYYITLATDLHLLDSFQAD
                     ELENNLNEVSYLLNSYARSIKRRKEGDYKKDDDLRDE"
     gene            2901..3779
                     /locus_tag="PLW22_11395"
     CDS             2901..3779
                     /locus_tag="PLW22_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOU75348.1"
                     /translation="MTTANIFKTVRSYAIIVFGLLLYALSWTAFLIPHKITGGGVSGI
                     GALVYYATGIPMGYTYFLINVGLILLAIKMLGANFGVKTIFGVTVGAFLLSLLQMTIK
                     VAVVEDKFMSTIIGGALAGVGLGVVFTQGGSTGGTDIIAMIINKYRNISPGRIIMLCD
                     VFIIGSSFLVLLDLEPAKRIETIVYGYVAMAITAYSLDAVLSGSKQSVQVFVFSKRYQ
                     EIADRITSEINRGVTVVDGMGWYTKESQKVLISLVRKHEVSDVYKIIKEVDPEAFISV
                     ATVTGVYGRGFERIRH"
     gene            complement(3776..>3964)
                     /locus_tag="PLW22_11400"
     CDS             complement(3776..>3964)
                     /locus_tag="PLW22_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HOU75349.1"
                     /translation="DEAGTAILKTAMERLNLSARAYDRILKVSRTIADLEQSEKILPQ
                     HVAEAIQYRSLDRENWAG"
BASE COUNT         1093 a          886 c          941 g         1044 t
ORIGIN      
        1 agatgttcaa gcactggtac aacaacaaaa cccaaaaggc tgagcccatt gagaacctta
       61 ttgctgagtt tgagcaaaac ggtaatatta atgttgatgc ggcctgtagc gaggttcgta
      121 cctttagcgc ctcggaatgg aaagccatga gcgaaaagga acagcaggag attttgcttg
      181 cttaccgcct agcctatctt gccgatgtta tggtaaactg gtgccctgca ctgggaacag
      241 tactggcaaa cgacgaggtt aaggagggtg tctccattcg tggcggccat ccggttgagc
      301 aacgcaaaat gcgtcagtgg tgtctcagga tatcggctta cgccgagcga ctactcaacg
      361 atcttgacca gctcgactgg actgactcgc ttaaggatat tcaacgcaac tggattggca
      421 aatccgaggg tgccgaggtt tggttccgaa ttgatggcac tgaccgtaaa atactaattt
      481 tcaccacccg tcccgatacc atttacggcg caacctttat ggtgttggca cccgaaagcg
      541 aactggttga tgagcttact acccccgagt atgctgagaa aatggagcaa taccgtcagg
      601 aggttaagcg aaaaaccgag cgtgagcgca tggccgaagc ccgcaaggta accggtcagt
      661 ttaccggtag ctacgccatt aacccattca ccaacgagcg tatacctatt tgggtaagcg
      721 aatacgtttt agcgggctat ggaaccggtg ccattatggc ggttcctgcc cacgatagcc
      781 gcgattttgc ctttgccaaa acctttgggc tacctattat ccaaaccgtt attcgcccag
      841 gcgagcaacc cactaaccct gaatcgtgga cagaatcgta cgattcaaag gagggtgttg
      901 ttatcaatag cccacttatc aatggcttag aggtaaaaga tgccatttca aagatatgtg
      961 aggtgattga atcgcgcggg ctgggcaagc gtaaggtaaa ctaccgccta cgcgatgcca
     1021 ttttcagccg ccagcgatac tggggtgagc cattccctat tttctacaag gacggaatgc
     1081 cctacccgct gcccgaggaa aaattacccc ttgaactccc cgagattgat gcctacctac
     1141 ccactgaaaa gggtgaacca cctcttggac gtgccaagaa ctggcatacc cctgagggat
     1201 acccctatga gctaagtacc atgcccggct ttgccggctc ctcggcatac tacctccgtt
     1261 acatggatcc ccacaacgat aatgcccttg tttcggccga agccaataag tattgggaaa
     1321 acgttgacct ttacattggc ggaaccgagc atgccgtggg acatcttatt tactcaaggt
     1381 actggaataa attcctatac gaccttggtt atgtttgcaa gcaggagccc tttaaaaagc
     1441 tgataaacca aggaatgatt cagggtcgat caaactttgt ttaccgtgtt aagggaacaa
     1501 accaatttgt atcgcacaac ctgaaggata agtacgatgt aaccccaata cacgttgatg
     1561 ttaacattgt aagcaacgat atacttgata ttgaggcatt ccgtaagtgg cgtccggagt
     1621 tcgagaatgc tgagttcatt ctggaagatg ggaaatacgt atgcggatgg gcagtggaaa
     1681 agatgagcaa gagtatgtgg aatgtggtaa accccgatac cattgttgag cgctatggtg
     1741 ccgataccct ccgcatgtac gagatgttcc ttggccccct agagcaaagt aaaccttggg
     1801 atacaaatgg tattgatggt gtacataagt ttctccgaaa gttctggagg ttattccata
     1861 acaatgccaa tgagtttgag gtatcagcca acgaacctac tgcacaagag ctcaaggttc
     1921 ttcataaaac cattaaaaag gttactgagg atattgagaa gtttagcttc aataccggag
     1981 tatcggcctt tatgatttgc gtaaacgagc tctccgattt gaaatgtaac aagcgcgcca
     2041 tacttgaacc gctaaccgtt ttaattgctc cctatgctcc acatattgcc gaggagctat
     2101 ggcatctgct tggacatgaa acatcggtaa atactgccgc ttggcctaag ttcaacgagg
     2161 agtacattaa ggaatcgacc tttacctgtc ctatatcgtt taatggtaaa acgcgcttta
     2221 cccttgaact accgttaaac cttaaggccg atgaggtaga aaaaattgtt ttagccgatg
     2281 aaaacacgct aaaatacctc gatggcaagc aacccaaaaa ggtaattgtg gttccaaaca
     2341 aaattgtaaa tattgttgtt tagtcaaata atatttttgc agatttcaaa aaagtagtaa
     2401 ctttagtaaa cccttattaa tcattattat acttacaaaa cattcaacag aaaggagtgc
     2461 gatatggaca aatcggacaa caaaaccaaa tcgttcaagg acttggtagt atggcaaaag
     2521 gcccacgctt ttgtgcttaa catttaccag caggtaaaaa tttttcccga cgattatcag
     2581 gccattgttg gcgacatgct atgtgaaagt gccactgcca ttgcaaccaa cattgttgga
     2641 ggctacaaga aaaaggatcg cgaggacaag ctactctttt taaccaccgc acaggaggcc
     2701 cttgaggagt gccgttacta cattaccctt gccaccgatt tacacctctt ggattcattc
     2761 caagccgatg agctagagaa taacctcaac gaggttagct acctgcttaa ctcttacgca
     2821 cgttccatca aacgtcgtaa ggaaggtgat tacaaaaaag atgatgattt aagagacgag
     2881 tagtaggaat tttttagaga atgactacag ccaatatttt taaaacagtc cgatcctatg
     2941 ccatcatagt gttcgggctg ttgctttatg cgctatcgtg gactgctttt ttgattccac
     3001 ataaaattac tggcggagga gtttcgggta taggtgcgct tgtttactac gctactggca
     3061 ttccaatggg ttatacctac tttttaataa atgtgggact catcctttta gccattaaaa
     3121 tgctgggagc caactttggg gtgaaaacca tttttggcgt tactgttggc gcatttctac
     3181 tatcgctgct ccaaatgaca ataaaggttg cagtggttga ggacaagttt atgtccacaa
     3241 tcattggcgg tgcgctggca ggtgttgggc ttggggttgt gttcactcag ggcggcagca
     3301 ctggtggtac cgatatcatt gccatgataa tcaataagta ccgtaacatt agcccgggcc
     3361 gtataataat gctttgcgat gttttcatca ttggctcatc gtttttggtt ctactcgacc
     3421 ttgagcccgc caagcgtatc gaaaccattg tttatggtta tgtggccatg gccatcacgg
     3481 cttactccct cgatgccgtt ctttcgggta gcaaacagtc ggttcaggtg tttgtctttt
     3541 caaagcgata ccaggaaatt gccgatagga ttacatctga gattaaccgt ggagtaaccg
     3601 tggttgatgg aatgggctgg tacacaaaag aatcgcaaaa ggtgctaatc tcacttgtgc
     3661 gcaagcacga ggttagcgat gtgtataaaa taattaagga ggttgacccc gaagcgttta
     3721 tttcggttgc aacggttacc ggggtttacg gaagggggtt tgaacgtatc aggcactagc
     3781 ctgcccagtt ttccctatcc aagctacgat actgaattgc ctcagccaca tgctggggca
     3841 atattttttc ggattgctca aggtcggcaa tggtacggga aacctttaga attctgtcgt
     3901 aagcccttgc tgaaaggttt agccgttcca tagctgtttt caggatggcc gttccggcct
     3961 catc
//
LOCUS       DAOOWJ010000166         3959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_22958_length_3959_cov_9.612449, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000166 DAOOWJ010000000
VERSION     DAOOWJ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22958_length_3959_cov_9.612449"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PLW22_11405"
     CDS             complement(<1..1206)
                     /locus_tag="PLW22_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU75350.1"
                     /translation="MEIKRTFDILEAGKDTFENRTVIAVKKNGVWDEYTIERYSQMAH
                     NLSYGLLSMGLRPGDKVANISGNRPEWNIVDMGITQAGMVHVPIYPTISTDEYKYILK
                     HAEVRAIFVGDKALYQKLQPLTADLENFIGIFSTVENGDIRSWNEVLERGKQTAHEWV
                     EELFKIKASVQPDDIATIIYTSGTTGVSKGVMLSHSNFLSNVEACKGLFPLENGDIAV
                     SFLPLSHVFERMVNYYYQSIGVSIYYADNLATIGDVIKEVKPHIFMTVPRMLEKTFDK
                     IIAVGKDLTGLKKQIFFWAVNLGYKYNEVRNSWYYRIRLNLARKLIFSKWQKALGGRL
                     KLIISGGAALQPRLMRVFWAAGIPVFEGYGLTETSPVIAVNHPASPGNIRIGSVGPVV
                     PNVKVKIADD"
     gene            1294..2169
                     /locus_tag="PLW22_11410"
     CDS             1294..2169
                     /locus_tag="PLW22_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOU75351.1"
                     /translation="MKQVSFCLIVCLISVNVFGQDKHAYTLFNGKGEKVVYQSVVSAV
                     KNSDLLLFGELHDNPIAHWLELELTVDVFDATKGNIILGAEMFEADNQLILDEYLGGL
                     ISQQRFEAEVRLWPNYKTDYKPLVEFAKSKGLKFIATNIPRRYAAMVSAGGFDTLQSL
                     SEMAKSFIAPLPVEYDPNLGCYKGMLSMMGMPGKGKSNPEYFPMAQAIKDATMAYNIT
                     RNTSSGKVFLHFHGSYHSNNYEGIYWYVKRKMPNLKIVTIATVTQDDVNALAKENMGI
                     ADFIIVVPERMTRTY"
     gene            complement(2174..2305)
                     /locus_tag="PLW22_11415"
     CDS             complement(2174..2305)
                     /locus_tag="PLW22_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75352.1"
                     /translation="MRTNLDRYSEYENNLLNRVTTLLIIVVITAILGLVCVENFLLR"
     gene            complement(2360..2770)
                     /locus_tag="PLW22_11420"
     CDS             complement(2360..2770)
                     /locus_tag="PLW22_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75353.1"
                     /translation="MKAKFFLISALGLSTLFGCIEGDNYVRFNDYVTMEAGLLPDTMI
                     LNEPYNLQIRASAPNGCWYNVKVYLNVKSDSAYFFSAAATYENHGEPCTQQLVTHDTT
                     ITITPKVPKTHLMYFYNPTGNPQIRIDTIYIKAE"
BASE COUNT         1028 a          838 c          852 g         1241 t
ORIGIN      
        1 gtcgtcggct atctttacct ttacattagg cactacaggg cctactgagc ctatcctaat
       61 gttgccgggt gaagcagggt ggttaactgc aattacaggc gaggtttcgg taagtccata
      121 tccctcaaaa acaggaatgc cagcagccca gaataccctc atgagtcggg gttgaagagc
      181 agctcctcca ctaatgataa gcttgagcct tccaccaagt gccttttgcc actttgaaaa
      241 gattaacttg cgggcaaggt taagcctaat ccggtagtac cagctattgc gtacctcgtt
      301 atacttgtag ccaaggttaa ctgcccaaaa gaaaatctgt ttctttagcc ctgttaaatc
      361 ctttccaacc gcaattatct tgtcgaatgt tttctcaagc atgcggggaa cagtcatgaa
      421 aatatggggt ttaacttctt taattacatc gccaatggtg gcaaggttat cggcatagta
      481 aatgcttacc cctatgcttt ggtagtaata gttcaccatg cgttcaaata catggcttaa
      541 tggaagaaag cttacggcaa tgtcgccgtt ttcaaggggg aatagtcctt tacaggcttc
      601 aacattacta aggaagttgg agtgtgaaag cattacccct tttgaaacgc ctgttgtgcc
      661 cgaagtgtaa ataattgtag caatatcatc gggttgaacc gatgctttta tcttgaaaag
      721 ctcctcaacc cattcatgtg cagtctgttt gccgcgctcc agcacttcgt tccagctgcg
      781 gatatcgcca ttttctactg ttgagaatat cccaataaaa ttctcgaggt cggcggtaag
      841 gggttgaagc ttttggtaca aggccttatc gcctacaaaa atggctctta cctctgcatg
      901 ctttagaatg tatttgtact catcggtgct gattgttggg taaatgggca catgaaccat
      961 tccggcctgg gtaataccca tgtcaacaat attccattca ggccgattgc cgctgatgtt
     1021 agcaacctta tcgccaggcc taagtcccat ggacagcaat ccatagctga ggttgtgagc
     1081 catttgagag tatcgctcaa tggtgtactc atcccaaacc ccatttttct taacagcaat
     1141 tactgttctg ttctcaaagg tgtccttgcc tgcttcaaga atgtcaaaag ttcttttaat
     1201 ttccatgctt tatcagataa agttttactt atttgaacta tttgctcaga aaaaggttaa
     1261 tttagtcccg gcgacaaata taaactaatt tctatgaaac aagtatcctt ttgtttaatc
     1321 gtttgtttaa tttccgtaaa cgttttcgga caggacaagc acgcctacac cctcttcaat
     1381 gggaaagggg aaaaggttgt ttaccagagt gttgtaagtg cggtaaaaaa ttctgactta
     1441 ttgttatttg gagagctgca cgacaaccct atagcgcatt ggcttgagct ggaattaacg
     1501 gttgatgttt ttgatgccac aaaagggaat ataatactgg gtgcagagat gtttgaagca
     1561 gataatcaac ttattctgga tgagtatctt ggtggcttaa ttagccagca acggttcgaa
     1621 gccgaagttc gcttgtggcc caactataaa actgactata agccactggt tgaatttgct
     1681 aaaagcaaag gtttaaaatt tattgccaca aatattccgc gccgctatgc tgctatggtt
     1741 tcggcaggtg gttttgatac gctacaatct ctatcggaaa tggcaaaatc gttcattgcc
     1801 cctttgccag tagagtatga cccaaacctg ggatgctata agggaatgct cagtatgatg
     1861 ggaatgcccg gtaaagggaa atcgaaccct gaatatttcc caatggcaca ggccattaag
     1921 gatgcaacta tggcatataa tataacccga aacactagta gcggtaaggt ttttctacat
     1981 tttcatggtt cttaccactc taacaactac gagggaatat actggtatgt aaagcgaaaa
     2041 atgccgaacc tgaaaattgt gacaattgca actgtaaccc aggatgatgt caatgctttg
     2101 gcaaaggaaa atatgggcat tgccgatttt atcatagttg tacccgaacg aatgacccga
     2161 acatactaat cccttacctg agcaggaagt tttcaacaca aactaaccct aaaatagctg
     2221 taataactac aattatcagc agggttgtta ctctgttcag caggttgttc tcgtattcag
     2281 agtatcgatc cagattagtt ctcatggcta tagtttcttt tacttatact acaaccatga
     2341 ggggttttac cctaaccggc tattcagcct taatgtaaat agtatcaatc ctaatttgag
     2401 gattaccagt gggattgtaa aagtacatta ggtgagtttt cggcactttg ggtgttatgg
     2461 ttattgttgt gtcgtgagta accaattgtt gggtacatgg ttcaccatga ttctcgtatg
     2521 tggcagctgc gctgaaaaag tatgcagaat cggatttaac gttcaggtaa accttaacat
     2581 tataccagca gccatttggc gccgatgccc ttatttgaag gttgtatggt tcatttaata
     2641 tcattgtatc gggcagtaat cctgcttcca tggttacata atcgttaaac cttacatagt
     2701 tatcgccctc aatacatcca aaaagggttg acaatcctaa cgctgaaatg aggaaaaatt
     2761 ttgctttcat tgagtttttc attagtattt ctgcaaattt atacaaaaat tgtaccgttt
     2821 ggctatttaa cctctaattt gttgtatggc gttggtgagg tatgaaaata ctactcaaaa
     2881 atttgtcatg ctgagctagc cgaagcatca cattgcgtct tcattctttt attcactttt
     2941 tgctctttgg aatgttctcc ttttgtgatg caacgtttca gtgttgctct atggaatgcc
     3001 atagcgtgca tcgctattcg tgtgtaaagc tatggtgtta caaatattac gcccctacgg
     3061 ggctgggaac gttgctcgtt tttcgttgtt cgtgaatcgt tgctcgttgt tcgtgaatgc
     3121 ctttttgtga aacactgtat tacacagtgt aactctgtga tgcttgtttt tactaagctc
     3181 attagacgat tgtcatgctg agctcgtcga agcatccctt tgctctattg cccttcaatc
     3241 ctttaatgag atccctcgac taagctcggg atgacaaaaa ccccatcatt ccatttgccc
     3301 ccttgtcttg ctgagttagc cgaagcatct ctttgtactt tcattccatt atatttatga
     3361 gattcctcca ccaacacgga atgacatagg cagtggcata aaagtctgac ccgacaatgg
     3421 gtcgggggtg gaacatggcg gtgggtttag taagcgatac cacatgagtc acgccgcagg
     3481 cgtggcgaat agtatcgcgt agcagttcca cccacggggc acccatcggg tcgggctttt
     3541 cagccttgat tttctttggt tctttcttgt atcaagacaa gaaagaacat gtatgccgct
     3601 ctttgctagg cttttccgct gcgatgcaac gtttgctcct tgctctttgg attatgtatt
     3661 gcgtgcatcg caacacttgt gggattagcc atagtgttac aaatattacg cccctacagg
     3721 gctggaaacg ttgttcgttg ttcgtgaatc gttgtttgtt gctcgtgaat gccgcgccac
     3781 atactctgtg taacttcgtg ttacatagtg taactctgtg atgcttgctg ttgctcggtt
     3841 catgagacgg ttgtcatgct gagcctagcc aaagcatctc tttgtatctt cattccctta
     3901 ttcacttttt gctctttgga atgttctcct gttgcgatgc aactattcag cgttgctct
//
LOCUS       DAOOWJ010000167         3957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_22972_length_3957_cov_5.378780, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000167 DAOOWJ010000000
VERSION     DAOOWJ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22972_length_3957_cov_5.378780"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2632
                     /locus_tag="PLW22_11425"
     CDS             <1..2632
                     /locus_tag="PLW22_11425"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOU75354.1"
                     /translation="DGKIKPYPYNNKIRQNMPTRSKGPIKMSFYIDSLDNVYLGVKDY
                     GIMHITPEGIFKKFDGINIEAPVVLNEFPNSKILISNPNKPIKHELIINSNTKTTRFP
                     LKDIFDGNNPVYTYAIKPNDSTLYASLEGKVACFVNHKLKWVDRQFKSIIWLSVNDEY
                     LITSDLEGGLNLSPLVNQNGLKYKKILKEFQVTSVIKDSEGSYWVSTLDNGVLYIPDI
                     HIQTITKKDGLSDNRIKTVYQQDGNLYLGYQMGWVDMIKDGKVEKIKMTKTEGNTSFV
                     RYFLGDEANGRIWICSFGHLGYIQNNRYKVIESKIPVYPRKIVKSKKGNYYWVSTAYG
                     IVKVQDDKVVYDSRDEGFSPLVLGIDEDSKGNVWFVTSNGLWKYSNKLFQFMGDENPL
                     LASPSYALYFNPKDESLWIGTNGAGIVILDKNGKIKQITKDDGLISNSIYAFFAHKNR
                     IWVGTRQGLSIITFNNGSYSLQNLTTNEGLVSNDITSLYVNDSNAYIGTTSGLSIINI
                     NRIKPNQTKPNIYITSIEVADSTYKWTPDGLNIPWDTKYAKISYEGLSYKNMGKALYR
                     YRLLGLDSNWVYTTSNQCLYSVFSPGHYSFEVKVQNSNGIWSNPASLSITVTPPYWKR
                     IWFIILFALTLSGFLYLIYYLRMGELKRRNELINNINLYKQQSLRQQMNPHFIFNTLN
                     SIQYYILEKDTISSHKYLTKFARLMRYTLDNSLHSAIPLRDELESLKLYLELEALRLQ
                     DKFTYDIDYQNNEAVLDIKIPTLMIQPFVENAIWHGIMLKPDKSGRIQITIRDMETSV
                     LCTIEDNGVGREQAKKIQETTNKVHRSRGFQITQQRIDLLNSMYGQKFNIQIVDLYSH
                     DGTPQGTRVTINIPKGLESS"
     gene            2987..>3957
                     /locus_tag="PLW22_11430"
     CDS             2987..>3957
                     /locus_tag="PLW22_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOU75355.1"
                     /translation="MLSKGVCWNTTGNPTISDYRNIDGKGIGEFTSTIKGLAPNTTYY
                     IKAYATNANGTTYSNSVSFKTQPELATISDISFTSVTKTAAQCSASIISAGGETVTER
                     GFCWSKSPMPTIANNKIALNGGIGSFSATLENLESGTQYYIRAYAITSAGTSYSVERI
                     FTTASENPALVDNDHLLLGNPTGATTNIVNANNYLMVKPQYCLSYNNSKRTANWVAWH
                     LNSSWLGSVSRQDDFRADYTLPDTWYHVTESDYYYSQYGFDRGHMCPSADRTATVADN
                     SATFLMTNMVPQSPNNNQIVWESFESYCRNLAKSGSELYIISGPYGEG"
BASE COUNT         1316 a          853 c          754 g         1034 t
ORIGIN      
        1 agatggaaaa ataaaaccct acccctataa taacaagata aggcaaaaca tgccaacccg
       61 gtcaaaaggg cctataaaga tgtcgtttta cattgattca cttgataacg tttatcttgg
      121 agtaaaagat tatggaataa tgcatattac tcctgaaggg atttttaaaa aatttgatgg
      181 tataaacata gaagccccgg ttgtgctaaa cgaattccca aatagcaaaa ttttgatttc
      241 aaatcccaat aaacccatta aacacgaact aattattaac tctaacacta aaaccactcg
      301 tttccccctg aaagatattt ttgatggaaa taatcctgtt tacacatacg caataaagcc
      361 aaacgattca acattatatg cgtccctaga gggaaaagta gcctgttttg ttaaccacaa
      421 actaaaatgg gtagatagac aatttaaaag cattatttgg ttaagtgtga atgatgagta
      481 tctcattact agtgatctgg agggtggact gaatctttca ccattggtaa atcaaaatgg
      541 attaaaatac aaaaagattc ttaaggaatt tcaggttact tcagtaatta aggactcaga
      601 aggctcttac tgggtttcga cactcgacaa tggcgtactg tatatacccg acatacacat
      661 tcaaaccatc accaaaaaag atggcctaag cgacaataga attaaaactg tttaccagca
      721 ggatggcaat ctttaccttg ggtatcaaat gggatgggtg gacatgatca aggatgggaa
      781 ggtagaaaaa atcaaaatga caaaaacaga ggggaatacc tcatttgtca ggtatttcct
      841 gggcgacgag gccaatggca ggatttggat ttgctccttt ggacatttgg ggtatataca
      901 gaacaatagg tacaaagtaa ttgaatcgaa aataccggtt taccccagaa aaattgtgaa
      961 aagtaaaaag ggcaattact actgggtttc tactgcctac ggaatagtaa aagtgcagga
     1021 cgataaagtg gtgtacgatt ccagagatga aggtttttcg cctcttgttc ttggaattga
     1081 tgaagatagc aagggaaatg tttggtttgt tacatctaat gggctatgga agtattccaa
     1141 taaactcttc cagttcatgg gcgacgaaaa tccactcctg gcatcaccca gttatgccct
     1201 ttactttaac cccaaagatg aaagcttgtg gattggaact aacggcgctg gtattgtaat
     1261 actcgataaa aatggcaaga taaagcagat aaccaaggat gatggcttaa tttcaaactc
     1321 aatatacgcc ttttttgccc acaagaacag gatttgggtt ggcacacgtc aggggttgtc
     1381 aataattact ttcaacaatg gcagttactc tcttcaaaac cttaccacaa acgaaggcct
     1441 tgttagtaac gatataacat cgctgtacgt taatgattca aatgcctata ttggaacaac
     1501 tagtggcttg tcaataataa acatcaatag aattaagcct aaccaaacga aacctaacat
     1561 atacataacc tcaattgagg tagccgacag tacctataaa tggacacccg atgggttgaa
     1621 tattccatgg gatacaaagt atgctaaaat ttcgtatgag ggcttatcct ataagaatat
     1681 gggcaaggcg ttataccggt accgcctact tggcctcgac tcaaactggg tttacaccac
     1741 atcaaaccaa tgcttatata gcgttttttc gcccggtcat tattcctttg aggtgaaagt
     1801 acaaaactca aacggcattt ggagcaaccc tgcctcgcta agtattacag taacaccacc
     1861 ttactggaaa agaatatggt ttatcatcct ttttgcttta acactaagtg gtttcctata
     1921 tcttatttat tacttacgaa tgggtgagct caaaagacgt aacgagctaa tcaacaatat
     1981 caatctttat aagcaacaat cgttacgcca gcagatgaac ccccatttta tcttcaacac
     2041 gctaaattcc attcagtact acatactgga aaaagatacc ataagttcac ataagtacct
     2101 taccaaattt gcccggctga tgcgctacac cctcgataat tcattacatt ccgctatccc
     2161 actccgtgat gagcttgaat cgcttaaact ttaccttgag ctggaagccc ttaggcttca
     2221 ggataaattc acgtatgata tcgattacca gaacaacgaa gcagttcttg atattaaaat
     2281 ccctaccctg atgattcaac cctttgtgga gaatgccatt tggcatggaa ttatgctcaa
     2341 gcccgacaaa tcgggacgca tacagataac aattcgcgat atggaaacct cagtgctttg
     2401 taccattgag gacaatggcg taggtagaga acaagccaaa aaaattcaag aaaccaccaa
     2461 taaagttcac cgctcaagag gatttcaaat tactcagcaa cgcattgacc tgctgaactc
     2521 catgtacggc caaaagttta acattcaaat agttgacctt tactcccacg atggcacgcc
     2581 acaaggaaca agggttacca ttaacattcc aaaaggtctt gaatcgtcat aggataaata
     2641 attattatca ccataattat caaacaattc aagaacattc cactactata ttaaacaaaa
     2701 agtgcatatt ttatctaaaa atcatcggca aaatataact taacaatact taccatgcta
     2761 aataattact tttgcacggt atagcataag ttgatattgg catgaaagta acagtcatta
     2821 actactcaaa ggttttatca tagtttttac cttactggga acaattacac tggttggttg
     2881 taatgaggat aaggctcttc caaagattac ggcagttgaa gtttcaacca tttacaccta
     2941 ttccgccatt tgtaatggca aggtactgga cgatggtgga acgcaagtgt taagtaaggg
     3001 tgtatgctgg aacaccaccg gaaacccaac catttccgac tacaggaata ttgacggtaa
     3061 gggaattggt gaattcacca gcacaataaa gggtcttgcc cctaacacaa catactacat
     3121 aaaggcttat gccaccaatg ccaacggaac cacatacagc aatagtgtta gttttaaaac
     3181 ccagccggaa cttgcaacca tttccgatat ttcattcaca tcagtaacta aaaccgcagc
     3241 ccaatgctcg gcaagcatta tctcggcagg aggtgaaacg gtaactgaaa ggggattctg
     3301 ctggagcaaa tcacctatgc ctaccattgc caataacaaa attgccctta atggcggcat
     3361 tggttccttt tcggctacat tagaaaacct ggaaagtgga acacaatact atatccgtgc
     3421 atacgctata accagtgcgg gaacatcgta cagcgttgaa cgaatcttta ccaccgcttc
     3481 cgaaaaccca gcactagttg acaacgacca ccttttactt ggcaatccaa caggtgcaac
     3541 caccaatatt gtgaatgcca ataactacct aatggttaaa ccccagtact gcttatcgta
     3601 caacaactca aaacgaactg ccaactgggt ggcatggcac ctaaactcca gctggctggg
     3661 tagtgtttcg cgccaggatg atttcagggc cgattacaca ttacccgaca catggtacca
     3721 cgtaaccgaa tcggactatt actattccca gtacggtttc gaccgtgggc atatgtgccc
     3781 cagcgccgac cgtaccgcca ccgttgccga taactcggca accttcctga tgaccaacat
     3841 ggtaccccaa tcgcccaata ataaccagat tgtgtgggag agctttgaaa gttactgccg
     3901 gaacctggca aaaagcggct cagagcttta cataatcagt gggccttacg gcgaggg
//
LOCUS       DAOOWJ010000168         3835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_23863_length_3835_cov_7.692857, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000168 DAOOWJ010000000
VERSION     DAOOWJ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23863_length_3835_cov_7.692857"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1938
                     /gene="ispG"
                     /locus_tag="PLW22_11435"
     CDS             58..1938
                     /gene="ispG"
                     /locus_tag="PLW22_11435"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOU75356.1"
                     /translation="MRSYIDSIADFKRRVTVEVMVGNTPLGGGNPIRVQTMTTTDTNN
                     TQATVEQCIRAIEAGAEFVRITTQGTREAANLQNIKGELIARGYTTPIVADVHFNPAA
                     ALEAAKHVEKVRINPGNYVDPRAKFETVSYTDQEYADELKRIEERLLPLIEICRERGV
                     AIRIGVNHGSLSDRIMSRYGDTPAGMVESAMEFLRIFRKHGFNQLVISMKSSNTRVMV
                     QAVRLLVATMDAEGMNFPLHLGVTEAGDGEDGRIKSAVGIGALLADGIGDTIRVSLTE
                     EPENEIPVAKKLVNYFANRKTSPSLKSIDSLPYNPYEYNRRVIPEVKGVGGANPPRVF
                     AYINETFTESTANAWGWRFDSVKKLWQPTDAAPDYFVVPGFSPSPNFDGLPVISEHTP
                     NFRLIKVDDFINQKSLGSVMLSVTYADLLNPDLVSALKGSQNVVLLLSTSNSNGFAEQ
                     RAAVIELMRQEICLPVVVARGYSDAEKEDFQLKAAADVGGLLIDGLIDGVMLTSPLSP
                     EVIRNTAFAILQAARTRVTKTEYIACPGCGRTLFSLQQTLQKVRSATSHLKGLKIGVM
                     GCIVNGPGEMADADYGYVGAGPGRITLYKGKEVVKKNIPEEQAIDELIALIKAHGDWV
                     DQ"
     gene            complement(1958..3349)
                     /locus_tag="PLW22_11440"
     CDS             complement(1958..3349)
                     /locus_tag="PLW22_11440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU75357.1"
                     /translation="MQSQEKHQESIVVLILVMFTSFITPFIGSAINLALPMIAHEFGL
                     NAVTMSWVTMAFLLSSAVFLVPLGKLADIFGRKRVFLTGNVVLALASLLAVFAPNDAT
                     LIALRVVQGIGSAMMFATGIALITSVFPPNARGRAIGMNVTAVYVGLSAAPVLGGLMI
                     DALGWRSLFYIPALLGIPAVIAAILTIKGEWAEAKGEKFDILGSGIFIVSMCALMYGF
                     SKLPSTVAIVLTAVGLLGLALFVKLQMKLEYPVFNVSLFRVNRLFALSNLAALINYAT
                     TFAITFVLSLYLQYIKGLSAREAGLLLVIQPLVMAVIASWSGRLSDKHDPRILASAGM
                     GVIAVGLALLIPISNETAVTYIGVVLGILGLGFGMFSSPNTNAIMGSVEKRYIGLASG
                     TVATMRLVGQMLSMGIATMVIHVFIGTGTINASNHKQFILSASVTFAIFVALSVLGVW
                     ASLARGGKNGNQA"
     gene            complement(3441..3785)
                     /locus_tag="PLW22_11445"
     CDS             complement(3441..3785)
                     /locus_tag="PLW22_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75358.1"
                     /translation="MGLIHSRSTIASLIFNGKMFHLLNPWHIEVDTNQETITIRKRNP
                     FLIGFDEQIVSFRYIRSIMIDEHLFGADIHVKVVGGRASAYCISKSDARRIKNTLLEY
                     NATKRGRGIIFS"
BASE COUNT         1076 a          888 c          917 g          954 t
ORIGIN      
        1 tctcaaccca ggtaatactg taaaaagtat ttactttgca gaataatact atttgccatg
       61 aggagctaca tcgatagtat agctgatttt aagcgcagag taaccgttga ggttatggtt
      121 ggcaacactc cgctgggcgg aggtaatcct atacgggtgc agaccatgac cactaccgat
      181 accaacaaca cgcaggcaac cgttgagcaa tgcattaggg caattgaggc aggagccgag
      241 tttgtgcgta ttactactca gggtacccgc gaggctgcta acctgcaaaa cataaagggc
      301 gaacttattg ccaggggcta taccaccccc attgtagccg atgttcattt taaccctgcg
      361 gcagcccttg aggctgccaa gcatgtggaa aaggtgcgaa taaatccggg caactatgtg
      421 gatccccgcg ccaagtttga aaccgtaagc tacaccgacc aggagtatgc cgatgagctc
      481 aaacgcattg aggagaggtt acttccgcta attgagattt gcagggaacg aggcgttgcc
      541 attcgcattg gggtaaacca tggctcactt tccgatagga ttatgagtcg ctatggcgat
      601 actccagctg gaatggttga gtcggccatg gagttcctgc gtattttcag aaaacatggg
      661 tttaaccaac tggttatctc catgaaatcg agcaataccc gtgtgatggt gcaagccgta
      721 aggcttttgg ttgccaccat ggatgccgag ggtatgaatt ttccgcttca ccttggtgtt
      781 accgaggctg gcgatggcga ggatggccgc attaaatcgg cagtgggaat tggagcgctc
      841 cttgccgatg gtattggcga taccattcgg gtttcactta ccgaggagcc cgaaaacgaa
      901 ataccggtag caaagaaatt ggtaaactac tttgccaata ggaaaacttc gcctagctta
      961 aaaagcatcg atagcctacc gtacaatccg tacgagtata accggcgggt aatacctgag
     1021 gttaaaggcg ttggaggagc aaaccctcca cgagtgtttg cttacatcaa cgaaactttt
     1081 actgagtcta ctgccaatgc gtggggatgg agattcgatt cggtaaaaaa gttatggcag
     1141 ccaaccgatg ccgcacccga ctactttgta gttcccggat ttagccccag tccaaacttt
     1201 gatggcttac cggttatttc ggaacataca cccaatttcc gcttgataaa ggttgatgat
     1261 tttatcaacc aaaagtcatt gggttcggtg atgttaagcg ttacctatgc cgatctgtta
     1321 aaccctgatc tggtatcggc actaaaggga agccaaaacg tggtactgtt gttatccacc
     1381 agcaattcaa atggatttgc tgagcagcgt gctgctgtga ttgagctgat gcgccaggaa
     1441 atttgcttgc ctgtggttgt cgcacgcggt tactccgatg ctgaaaagga agattttcag
     1501 cttaaagctg ctgccgatgt gggaggacta ctgattgatg ggctgattga cggggtaatg
     1561 ttaacttcgc cactatcccc tgaggtgata aggaatacag catttgccat tctgcaggct
     1621 gcccgaacac gagttaccaa aacggaatat attgcttgcc ctggctgtgg acgcacgcta
     1681 ttcagcctgc agcaaacgct acaaaaggtt aggagcgcta cctcgcacct gaaagggtta
     1741 aaaattgggg taatgggttg tatagtaaat ggcccgggcg aaatggccga tgccgattac
     1801 ggctatgtgg gtgcaggccc cggcaggatt accctttaca agggcaagga ggttgtaaag
     1861 aagaatattc ctgaggaaca ggctattgat gagctaattg cattaattaa ggcgcatggc
     1921 gattgggttg accaataaaa ttgatgttga aatgctttta tgcttgattt ccgttctttc
     1981 caccccttgc cagcgatgcc caaaccccta aaacgcttaa ggctacaaat attgcaaacg
     2041 taacactggc cgaaagaata aactgcttat ggtttgatgc gtttatggtt cccgtgccaa
     2101 taaatacgtg gattaccatg gtggcaatac ccatgctcaa catttggcca accaaccgca
     2161 tggttgccac cgtccccgat gcaagcccaa tgtaacgttt ttcaaccgag cccataatgg
     2221 cattggtatt gggcgatgag aacattccaa aaccaagccc cagaatgccc agaaccaccc
     2281 caatgtaggt aactgctgtt tcgttactta taggaataag cagcgccaaa cctactgcaa
     2341 ttactcccat gcctgccgat gccagtatgc gtggatcgtg cttatcggaa agccgccccg
     2401 accacgatgc tataaccgcc ataacaagcg gttgaataac cagtagcaag ccagcctcgc
     2461 gcgcacttaa acccttgatg tattgcaggt aaaggcttaa aacaaaggtt atggcaaagg
     2521 tggttgcata gtttatcagc gcagcaaggt tcgaaagcgc aaaaagccta ttgaccctga
     2581 aaagcgatac gttaaaaacc gggtattcaa gtttcatctg cagcttaaca aaaagggcaa
     2641 ggcctaaaag gcctactgcg gttaggacta tagcaacagt ggatgggagt tttgagaacc
     2701 catacatcag ggcgcacatc gatactataa atatgcctga ccctaaaata tcgaactttt
     2761 cgcctttggc ttcggcccat tcgcctttaa tggtaagtat agcagcaatc acggcaggta
     2821 tgcccagcaa tgctgggatg tagaacaaac ttcgccaccc cagtgcgtca atcattaaac
     2881 caccaagaac tggcgcagcc gaaaggccaa catataccgc agtaacgttc attccaatag
     2941 ctctacccct tgcgttgggc gggaaaactg atgtgataag ggcgatgcca gttgcaaaca
     3001 tcatggcgct tccaattccc tgaacaaccc gcaatgctat gagtgttgcg tcattgggcg
     3061 caaatacggc caaaagcgat gccaatgcaa gcactacatt accggtaagg aaaactcttt
     3121 tccgtccgaa tatgtcggcc aacttaccta ggggcaccag aaatactgcc gacgagagca
     3181 gaaaggccat ggtcacccag ctcatagtaa cagcatttag cccaaactca tgggcaatca
     3241 ttggcaaagc caggtttatt gccgacccaa taaagggagt aataaacgat gtaaacatta
     3301 ccaggatgag cactactata gattcctgat gtttttcctg cgattgcata taaaagattc
     3361 cttggggtaa ttacaacaaa attgtagccc caaagtaaat caaaaaaaat aaacttaagt
     3421 tttttagcga gatatctgct ctatgaaaag attattcccc ttcccctttt tgtggcattg
     3481 tattccagga gcgtattttt aattctcctt gcatcgctct tggaaatgca gtaggccgag
     3541 gctctgcctc ctactacctt tacgtgaata tcggcgccaa acaggtgctc atctatcatt
     3601 atactgcgga tatacctgaa cgatactatt tgctcatcga accctatcag gaatgggttg
     3661 cgcttacgta tggtaatggt ttcctgattg gtatctacct caatatgcca ggggtttagc
     3721 aggtggaaca ttttaccgtt gaatatcaga ctggcaatgg ttgaccgtga atgaattaat
     3781 cccaaatccg tcaataggaa taaaatctct aatgacggcc attagagatt ttgtt
//
LOCUS       DAOOWJ010000169         3832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_23890_length_3832_cov_7.754832, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000169 DAOOWJ010000000
VERSION     DAOOWJ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23890_length_3832_cov_7.754832"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..989
                     /locus_tag="PLW22_11450"
     CDS             72..989
                     /locus_tag="PLW22_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75359.1"
                     /translation="MIIAIIDMGTNTFNLLVAQTSGKILHESKLPVKLGEGGFRSGSL
                     TPEAMQRAFKAINTHLITAKRLGANRCFAFATSAVRDASNGQEFVDEVKQRFNIDVEV
                     IDGNREAELIWKGVNAAIGLGKSPAVVVDIGGGSNETILANSEQIFWLQSFNLGVTRI
                     AEGFPISDPITTHELEAIESHMLQTVEPLLLAAANLNPRVMAGSSGAFDSFRKILAHK
                     GIIHDTSKFSAEIPVDEYLKLHRFFVTSTHAQRLALPGLDPIRVDLIIPASIFVNLLL
                     QKLGIKRMFQSDYALKEGFWVELSNKDKG"
     gene            1150..2313
                     /locus_tag="PLW22_11455"
     CDS             1150..2313
                     /locus_tag="PLW22_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU75360.1"
                     /translation="MSKILVIDDEKAIRNTLKDILEVEGHSVDVAEDGHAGLEMFDNG
                     AYELVLCDIKMPQMDGIEVLEKLQERQPDVPVVMISGHGNIDTAVDAIKKGAYDFIEK
                     PLDLNRLLITIRNATDKTVLIQEAKTLKRKVSKAFDIVGQSPAIVKVKEMIDRVAPTE
                     ARVLITGPNGTGKELVARWLHEKSNRAAMPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRKGKFEQADGGTLFLDEIGDMSLSAQAKVLRALQENKISRVGSDKDITVNVRVI
                     AATNKNLTQEIEKGNFREDLYHRLSVIIINVPPLCDRLEDIPLLAQHFNEQICAEYGI
                     PPKVINPDAIEELQKLKWTGNIRELRNVIERLIILCDKQITGNDVKAFVGPVF"
     gene            complement(2381..>3832)
                     /locus_tag="PLW22_11460"
     CDS             complement(2381..>3832)
                     /locus_tag="PLW22_11460"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOU75361.1"
                     /translation="QKLVKLEGVQFWNPAATYSIPGVNTNRILVDQNGNQIILRNSGY
                     AKFTKDQVPTGSGSITGVLSIFGSTYQLLIRDTLDVKFNQPRFGSEVPAKNTTIEQLK
                     LFYDGSALYQIPYDLVIEGIVTANDESGNLYKQLFIEDETGAIEFKVDVTDLYADFPV
                     GTKIRVKCKDLYMGTYGGVIQLGGNFGGQIGRLAASDFYKNVFVISSGNDVSVTETSI
                     GQLSNILIGKVVTLSGVQFADYELGKTYAESSSTTNRILEEVATGKTIIVRTSNYADF
                     AGVELPKGRGKITAILTKYNSDFQLTIRKVGEVRLIEPRLIKNFILNQDFTLATVNNP
                     ISINGWQTIAVAGTKVWNAKQYSGNVYARMSAFQSNESSNIAWLISPKITIPSGKDVF
                     FKFDTEYSFWANAILEVYISKNYDGTNPGTAEWTKLDATIANQSVGQYSWVNSGLIKL
                     NDFAGDAYIGFKYTGSNTLSTTFDVDNVSVYNF"
BASE COUNT         1090 a          778 c          885 g         1079 t
ORIGIN      
        1 ttggccgagg agaggcatag gtttatggaa agttttttag agcaattcta cagggagtgg
       61 aactataaag gatgattata gctattatcg atatgggcac caataccttt aacttattgg
      121 tggctcaaac ctctggaaaa attcttcatg agagcaagtt acctgtaaag cttggcgagg
      181 gcggttttag gagcggtagt cttacacccg aagccatgca aagggctttt aaagctataa
      241 acacacacct gattactgca aaacgcttag gcgctaaccg ttgcttcgca tttgccacgt
      301 cggctgtgcg cgatgcatcg aatggtcaag agtttgtaga tgaggttaag caaaggttca
      361 acatcgatgt tgaggttatt gatggtaatc gggaggctga gctgatatgg aagggggtaa
      421 atgcagctat aggtttaggt aaaagtccag cagttgtggt tgacatcgga ggcggcagca
      481 acgagaccat tttagccaac tcggaacaaa tcttctggtt gcaaagcttt aaccttggcg
      541 ttacccgaat tgccgagggt ttcccaattt ccgatcccat tactacccat gagcttgaag
      601 ccattgaaag ccacatgctt caaacagttg aaccattgtt actggcggca gcaaacctaa
      661 atcctagggt tatggctggt agttcaggcg cgttcgattc tttcagaaaa atactagcgc
      721 acaaagggat tatacatgac acatcaaagt tttcggccga aatacctgtt gatgaatact
      781 tgaagctaca tcgctttttt gttacgtcaa cccacgccca aaggcttgca ctgccagggc
      841 ttgaccccat aagggtcgat ttgattattc cagcatcgat atttgtaaac ttactgttac
      901 aaaagcttgg catcaaaaga atgttccagt ccgattacgc cctgaaggaa ggattttggg
      961 tggaacttag taataaggat aaagggtaaa gaataaagga tataggataa aggataaagg
     1021 tttcaactac ccgttctgaa attacgaatt attggattga aacgagcagt ttttttgggg
     1081 ttttggggct ggaactgtta gctggtaaca tctaactatt ttttttatct ttacaccaaa
     1141 atactggtta tgtccaaaat attggtaatt gacgacgaaa aggctattcg taatacactg
     1201 aaagatatcc tggaggttga agggcatagt gttgatgttg ccgaggacgg tcatgccgga
     1261 cttgagatgt ttgataatgg tgcctatgag ttggtgcttt gcgatatcaa aatgccgcag
     1321 atggatggaa tagaggtgct tgagaagtta caggagcgtc agcccgatgt acccgtggta
     1381 atgatttccg gacatggtaa tatcgacacg gctgttgatg caatcaaaaa gggggcatac
     1441 gatttcattg aaaaacctct cgatcttaac cgcttgctaa ttaccattcg gaatgcaacc
     1501 gataaaacag ttctgattca ggaggccaag acgcttaagc gaaaggtttc caaggctttc
     1561 gatattgtag ggcaatcgcc cgcaatagta aaggttaagg agatgattga ccgcgtggca
     1621 cccaccgagg ctcgtgttct aataactggc cccaatggaa ctggtaagga actggtagcc
     1681 cgctggttac acgagaagag taaccgggca gccatgccgt ttgtggaagt taactgtgct
     1741 gcaatccctt cggaattgat tgagagcgaa cttttcggac atgagaaagg cgcattcacc
     1801 tcggccataa agcagcggaa gggtaagttt gagcaggccg acggtggtac cctattcctc
     1861 gatgaaattg gcgatatgag ccttagcgcc caggcaaaag tgcttagggc tttgcaggag
     1921 aataagatta gcagggtagg cagcgataag gatattactg taaatgtgcg tgtaattgct
     1981 gcaaccaata aaaaccttac tcaggaaatt gaaaagggaa actttcgcga ggatttatac
     2041 caccgcctta gcgttataat tattaatgtg ccgccccttt gcgaccggct tgaagatata
     2101 cccctccttg ctcagcactt caacgagcag atttgcgccg agtatggtat cccacccaag
     2161 gttattaacc ccgatgccat tgaagagctc cagaagctca aatggactgg gaatattcgt
     2221 gagttgcgaa acgttattga gcgtttgatc atactttgcg ataagcaaat aaccggtaat
     2281 gatgttaaag cctttgtcgg gccagtgttt taaggtaatg ggagtaggtt aaataggaaa
     2341 ggccgggttt ccccggcctt tcctatcttt ttaatgctta ctaaaagttg taaactgata
     2401 cgttatcaac atcaaaggtt gttgaaagcg tattactacc tgtgtattta aaaccaatat
     2461 aggcatcacc tgcaaaatca ttaagcttta tcaatcctga atttacccat gagtactggc
     2521 caaccgattg atttgcaatt gttgcatcga gctttgtcca ttctgcagtc ccagggttag
     2581 ttccgtcata gttcttggat atgtatacct caaggatagc attagcccag aaggaatact
     2641 cggtatcaaa tttaaagaaa acgtctttcc cgcttggaat ggtaattttt ggtgaaatta
     2701 accaggcaat attactactt tcgtttgact ggaaagcact cattcgtgca tatacatttc
     2761 cactatattg cttggcattc caaacctttg ttccagctac agcaatggtt tgccaaccgt
     2821 taatgctgat tgggttattt accgtggcta gggtgaaatc ctggttcaaa atgaaatttt
     2881 tgatcaacct aggttcaatt aagcgaacct ccccaacttt acggatggta agctgaaaat
     2941 cgctgttata ctttgtaagg atggctgtga ttttgccacg gccttttggt aactcaacac
     3001 cagcaaaatc tgcatagtta cttgttctaa caataatggt tttgccggta gccacctctt
     3061 caagtattct atttgtggtt gaagaactct cagcgtaagt tttacccagc tcgtaatcgg
     3121 caaactgaac tcctgacagg gtaacaactt tgccaattaa aatgttgctt agctgaccaa
     3181 ttgaagtttc ggttacgcta acatcattac cgctactaat tacaaaaacg tttttgtaaa
     3241 aatcgcttgc cgcaagcctt cctatttggc caccaaaatt accgccaagt tgtataacac
     3301 caccgtaggt gcccatgtaa aggtccttac attttaccct gattttagtc cccacgggga
     3361 aatcggcata aagatcggta acatcaacct taaactcaat ggcacccgtt tcatcttcaa
     3421 taaagagctg tttgtaaagg tttcctgact catcgtttgc cgtaacaatc ccttcaataa
     3481 ctaaatcgta tggtatttgg tatagcgccg aaccgtcata gaagagctta agctgttcta
     3541 tggtagtgtt tttagctggc acctctgaac caaatcgggg ctggttaaac ttaacatcaa
     3601 gggtgtcgcg aataaggagc tggtaggtgc taccaaatat gcttagaaca ccggttatgg
     3661 aaccactacc ggttggaact tgatccttag taaactttgc gtaaccgcta tttcgcaaaa
     3721 tgatttggtt accgttctgg tcaacaagta tacggtttgt attcactccg ggaatactat
     3781 aggttgcggc agggttccag aattgaacac cctcaagctt aacaagtttt tg
//
LOCUS       DAOOWJ010000170         3821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_23991_length_3821_cov_5.832183, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000170 DAOOWJ010000000
VERSION     DAOOWJ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23991_length_3821_cov_5.832183"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLW22_11465"
     CDS             complement(<1..634)
                     /locus_tag="PLW22_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOU75362.1"
                     /translation="MYRSHTCGELRIAHVGSNVTLSGWVQRIRNLGAMTFIDLRDRYG
                     ITQVVVDDSSSSDVKEVVKELGREYVIQISGEVVERASKNSKLPTGDIEVKAKIVKIL
                     NTSEIPPFTIEDETDGGDELRMKYRYLDLRRNPVKNALLLRHRMAQEVRKYLDAQGFI
                     EVETPFLIKSTPEGARDFVVPSRMHPGEFYALPQSPQTFKQLLMVAGFDKY"
     gene            721..1164
                     /locus_tag="PLW22_11470"
     CDS             721..1164
                     /locus_tag="PLW22_11470"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOU75363.1"
                     /translation="MENTKDHEFFMREALKEAQKALAKDEVPIGAVVVCNGRIVSRAY
                     NLTETLGDPTAHAEMQAITAATNYLGGKYLNQCTLYVTIEPCPMCAGALYWAQVGQLV
                     YGAPDPKRGFSLIAKNILHPKTKTISGVLANECSNMITTFFNKKR"
     gene            1254..2225
                     /locus_tag="PLW22_11475"
     CDS             1254..2225
                     /locus_tag="PLW22_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU75364.1"
                     /translation="MRVRLVKQFLLLGIFALGAISVSGQSIKDAIQAYNNAATNYKQD
                     PEKALQDIQSALQISEQLGEEGSETKQNCEELIPTIYFQVAMAKYKAKDLKATLENLE
                     KAKESAIKYNDGATKTRVEKTLPKLYNAMGNNLFKEGKFDEAIANYEKSIKLDPNYID
                     PYLGTALAYEGLKNEEKMVEYLDLTIDLGGKANDAANVEKAKTKGKAYFLKKADEALN
                     ANKLNDVVKLLDMALKYDGDDYNIYRSMTINYSKLERWNDAIEYGNKALELVKGTADD
                     KAEIYFLIATAYHKLNKINEACAAYKNAAFGNFKPNADYQIQQLKCQ"
     gene            2296..>2510
                     /locus_tag="PLW22_11480"
     CDS             2296..>2510
                     /locus_tag="PLW22_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOU75365.1"
                     /translation="MPSRFRIVFSDVDGTLLNKNRELASATIYQIRKLTEHNIPFVMV
                     SARMPAGMIHLYREVPVDNPAICYNGAL"
     assembly_gap    2511..2610
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2611..>3821
                     /locus_tag="PLW22_11485"
     CDS             <2611..>3821
                     /locus_tag="PLW22_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOU75366.1"
                     /translation="MTELELLAPAKDLETGIAAITHGADAVYIAAHKFGAREKAGNPI
                     EDIAELCHFAHAYHARVYVTVNTIIFDSELKEVQELIHEIYNASADAIIIQDFAILAM
                     DIPPIAIHASTQMHNIDPNHINFLEKNGVSRIVLPRELNLGEISNIRSKTKAELEFFV
                     HGALCVSYSGQCYMSYLLSGRSANRGACAQPCRLAYDLVDEMGNVLVENKHLLSLKDI
                     NLSSHLADLIRAGITSFKIEGRLKDITYVKNITAHYSNLLNNFIGLNQQYKRASSGKC
                     TYTFTPDPERTFNRGFTTHFINSRKPNQASLNTPKSVGKRIGMVDRIYRNYFCINSAE
                     TIHNGDGLCFFNSQNQLVGFRVNKVDGDKVFPNQMPTDLTIETTIYRNEDFELEQTLN
                     RKSAERKVDCN"
BASE COUNT         1170 a          832 c          733 g          986 t
ORIGIN      
        1 aatacttgtc aaaacctgct accattagta gctgcttaaa ggtttgaggc gattggggta
       61 aagcgtagaa ttcacccgga tgcattcgcg aggggacaac aaaatcgcgg gcaccctcag
      121 gtgtcgattt aatgaggaac ggcgtttcaa cctcaataaa accttgtgca tcaaggtatt
      181 tccgaacttc ctgagccatt ctgtggcgca atagtaaggc gttctttacc gggtttctac
      241 gtaaatcaag atacctatac ttcattcgta attcgtcgcc accatcggtt tcgtcctcaa
      301 tggtaaatgg gggtatctca ctggtattga gtatttttac tatttttgcc ttaacctcaa
      361 tatctccggt ggggagtttg ctgttcttgc ttgcccgttc caccacctcg cccgaaatct
      421 ggataacata ttcacgacca agctccttga caacctcctt gacatcgcta ctagagctgt
      481 cgtcaacaac tacctgtgtt attccatacc tatctctaag gtcaatgaag gtcattgcac
      541 caaggtttcg gatacgttgc acccatccac taagggtaac attgctgcct acatgagcaa
      601 ttctaagttc tccgcaagta tgtgatctgt acatatctaa taccattttc aggttgcgaa
      661 tgtaaaaata aaaaacgatg cttacgctat attttttgat atatttggcc aaaatcttat
      721 atggaaaaca caaaggatca tgagtttttc atgcgcgaag cactaaagga agcgcaaaaa
      781 gcactggcaa aagacgaagt gccaattgga gcggtagttg tatgcaacgg ccgaatagta
      841 tcgcgtgctt acaatttgac cgaaaccctt ggcgacccaa cagcacatgc cgaaatgcaa
      901 gccattaccg ctgcaaccaa ctacctggga ggtaaatatt taaatcaatg tacgctttat
      961 gttaccattg agccctgccc aatgtgtgcg ggtgccctat actgggctca ggttggtcaa
     1021 ttggtttacg gtgcccctga cccaaaacga ggattttcac taattgctaa gaatatactt
     1081 catcccaaaa caaaaactat cagcggagtt ctcgccaatg aatgttccaa tatgattact
     1141 acatttttta acaaaaaacg ataattttat ggcttaattt ttgtttttta aatggatgtg
     1201 atatttttga ggccattaac aaattttaaa acacaattta aaaccaaata cctatgagag
     1261 taagattagt gaaacagttt ttgctattgg gcatttttgc attgggtgcc atttcagtaa
     1321 gcggacaaag cattaaagat gccattcagg catataacaa cgccgctaca aactacaagc
     1381 aagatcctga aaaagcatta caggatattc aatcggcact acaaatttcc gagcagttgg
     1441 gagaagaggg ctctgagacc aagcagaatt gcgaggagtt aatccccacc atttactttc
     1501 aggttgcaat ggccaagtac aaagcaaaag atctaaaggc cacccttgaa aaccttgaaa
     1561 aagctaagga atctgcaatc aaatataatg atggcgcaac taaaacccgg gttgaaaaaa
     1621 ctcttcctaa gctttacaac gcaatgggca acaacctatt taaggaaggt aagtttgacg
     1681 aggctatcgc aaactacgaa aaatccatta agttagatcc taactatatt gatccctacc
     1741 tgggaacagc cttggcatac gaaggcctta agaacgaaga aaaaatggtt gagtacctcg
     1801 accttaccat cgatttaggc ggaaaagcca acgatgccgc taatgttgaa aaggctaaaa
     1861 caaaaggcaa agcgtatttc ctgaagaaag ccgacgaagc tctcaatgcc aataaactaa
     1921 acgatgtggt taaactgctc gacatggctt taaagtacga tggcgacgat tacaacattt
     1981 accgctcaat gaccataaac tacagcaagc ttgaacgctg gaacgatgcc attgagtatg
     2041 gaaacaaagc acttgaacta gtaaagggaa ccgccgatga taaggctgag atttatttcc
     2101 tgattgctac tgcttaccat aaactcaaca agattaatga ggcttgtgcc gcttacaaaa
     2161 atgcagcctt tggcaacttt aagcccaatg ccgattacca gattcaacaa ctaaaatgcc
     2221 agtaacacaa ctttttacta tctttgaggg ctgcctttgc agcccttttt ttattgctat
     2281 tcaaaccttt tatcaatgcc aagtagattt cgaattgtat tttccgatgt cgatggaacg
     2341 cttcttaata aaaatcgtga acttgcatcg gctacaatat accaaatccg caaactaaca
     2401 gagcataata taccatttgt aatggtatcg gcacgaatgc cagcaggcat gatccacctt
     2461 taccgcgaag tgcccgtgga caaccctgcc atttgctaca acggtgcgct nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgaccgaac tcgagctact tgcccccgca
     2641 aaggatttag aaactggcat tgctgccatt acacacggtg ccgatgcggt ttacattgct
     2701 gctcataagt tcggtgcccg cgagaaagcc ggaaacccca ttgaggacat tgctgagctt
     2761 tgccattttg cccatgcata ccatgccaga gtttatgtta ccgttaacac tatcattttt
     2821 gatagtgagc taaaagaggt gcaggaactc atccatgaaa tttacaatgc cagtgccgat
     2881 gcaataatca ttcaggattt tgccattctt gctatggata ttccccctat tgccatacat
     2941 gccagcaccc aaatgcacaa cattgaccca aaccatatta actttcttga aaaaaatggc
     3001 gtatcgagga ttgttttgcc aagggaatta aaccttggtg aaatatcaaa tatccgcagt
     3061 aaaacaaaag ctgaacttga attttttgtt catggggcgc tgtgtgtttc atacagcgga
     3121 caatgttaca tgagctactt gctttcagga cgaagcgcca atagaggcgc atgcgcccaa
     3181 ccctgccgct tagcctacga ccttgtcgat gaaatgggaa acgttctggt ggaaaacaaa
     3241 caccttctat cgctcaagga tattaacctt agcagccacc ttgccgattt gattagagca
     3301 ggaataacct cgttcaagat agagggtcgc ctaaaagata ttacttacgt aaaaaatata
     3361 actgcacact acagcaacct gctgaacaac tttatcggtc taaaccaaca atacaaaaga
     3421 gcttcgagcg gaaagtgcac ctacaccttt acacccgatc ctgaacgaac ctttaaccgc
     3481 gggtttacca cccattttat taatagtcga aaacctaacc aagcatcatt aaacacccca
     3541 aaatcagtag ggaaaagaat tggtatggtt gaccgtatat accggaacta cttctgtatt
     3601 aattcagctg aaaccataca taatggcgat ggattgtgtt tttttaactc tcaaaatcaa
     3661 cttgtgggat ttagagtcaa taaagtagat ggcgataagg tatttcctaa ccagatgccc
     3721 actgacctta ctatagaaac aactatttac cgtaacgagg attttgagct ggaacaaacc
     3781 cttaatcgta aaagcgccga gcgtaaagtt gattgcaata t
//
LOCUS       DAOOWJ010000171         3754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_24538_length_3754_cov_5.919978, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000171 DAOOWJ010000000
VERSION     DAOOWJ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24538_length_3754_cov_5.919978"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLW22_11490"
     CDS             <1..294
                     /locus_tag="PLW22_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75367.1"
                     /translation="AIAGIIDVIPMILQKLTWDANLSAFSLWVVVGYLLSVTEIEMPT
                     WLKGSLLAFLILLPSAILIGSKEPFSLIPITILTLILGSLSGYTYHKVIEKLS"
     gene            330..785
                     /locus_tag="PLW22_11495"
     CDS             330..785
                     /locus_tag="PLW22_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOU75368.1"
                     /translation="MSLEERINQDIKAAMMAKDKVRLEALRAVKSAILLAKTDGEHKD
                     SLSADVEMKILQKQVKQRKDAAEQYIQAGRKELADKELAEASVIEEYLPKMLSADELK
                     AELQKIIAEVGAKAPSDMGKVMGVASKALAGKAEGKAIADMVKQLLAGN"
     gene            complement(782..1117)
                     /locus_tag="PLW22_11500"
     CDS             complement(782..1117)
                     /locus_tag="PLW22_11500"
                     /inference="COORDINATES: protein motif:HMM:NF021468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HOU75369.1"
                     /translation="MGEFKYKIRPEDVKDLIPSGRYCYASDKITVDGEPVGYMHRERP
                     EETEDSGWRFYSGTEDEEYVENPYHFMMFDLNYIANCDPAIIPYLKLKTGSELERIEG
                     TDKFRPVED"
     gene            complement(1130..1771)
                     /locus_tag="PLW22_11505"
     CDS             complement(1130..1771)
                     /locus_tag="PLW22_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOU75370.1"
                     /translation="MNSSRKRIKKYEEIVNSTSHGVGIVLAIAATVLLVIRAALHGTA
                     WHIVTYSIFGAGLINLYTASTLFHSAINPRVKFNLNKFDHSSIYILIAATYTPFALLS
                     IKGWLGWTVFGLVWGMAVAGVVFKLWFYSVKYRSVSAWLYIAMGWIGLIAVVPIIKNT
                     PNLSLWFLLSGCLAYSSSVIFYLKDHYAFVHGIFHLFIILGSACHFFALYFLI"
     gene            2088..>3754
                     /gene="lepA"
                     /locus_tag="PLW22_11510"
     CDS             2088..>3754
                     /gene="lepA"
                     /locus_tag="PLW22_11510"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOU75371.1"
                     /translation="MENIRNFCIIAHIDHGKSTLADRLLELTNTLSDRELQEQVLDSM
                     DLEREKGITIKSHAIQMEYTFQGKQYKLNLIDTPGHVDFSYEVSRAIASCEGALLVVD
                     ATQGIQAQTISNLYLALNHDLEIIPIINKIDMDAAMVDDVKDQIVDLLGCKRDEIIPV
                     SAKTGEGVDKVLEAIVTRVKPPVGDPNAPLQALIFDSVFNSFRGIIAYYKVFNGTIRK
                     GDKVKFFNTGREYEADEVGILRLKLQPRDYISAGDVGYIISGIKESNEVKVGDTITHV
                     ERPCDKAIAGFEDVKPMLFAGLYPVDADEYEDLRASLEKLKLNDASITFEPESSLALG
                     FGFRCGFLGLLHMEIVQERLYREFDMDVITTVPNVSYLVHTTKGEVVEVHNPSGLPAQ
                     TTIDYIEEPVISAQIITKSEYLGAVMKLCIDKRGILKNQVFLTSDRVELSFRLPLSEI
                     VFDFYDKLKSISRGYASFDYVMDGYQEAKLVKLDILLNGEMVDALSSLIHQDHAYDFG
                     RKMCEKLKELIPRQQFDIAIQAAIGSKIIARETVKALRKDVTAKCYGG"
BASE COUNT         1096 a          841 c          811 g         1006 t
ORIGIN      
        1 gctatagccg gtataatcga tgttattccc atgatactcc agaagctgac ttgggatgcc
       61 aacctgtcgg ctttttcgct ttgggtagtg gtaggttact tactttccgt tactgaaatc
      121 gaaatgccta catggcttaa gggatcatta cttgcttttc tgattctttt accctctgca
      181 attctaatag gctcaaaaga accgttcagc ctaattccta taacaatcct tactttgatt
      241 ttgggttcat tatcgggcta tacttaccat aaagttattg aaaaacttag ctaactttgt
      301 aggggaaacc atcaaaattc atctttacca tgtcattaga ggaacgcata aatcaggata
      361 tcaaggcagc tatgatggca aaggataagg tgcgccttga ggctctaaga gccgttaaaa
      421 gtgccattct acttgccaaa accgatgggg aacacaagga ttcactatcg gctgatgttg
      481 aaatgaaaat cctgcaaaag caggtcaagc aacgtaagga tgctgccgaa cagtacattc
      541 aagccggacg caaagaacta gccgataagg aattggccga agcctcagta attgaggaat
      601 accttcccaa gatgctcagt gctgatgagc taaaggctga actccaaaag attattgctg
      661 aagttggagc aaaggctcct tccgatatgg gaaaggtaat gggagttgca tcaaaggcac
      721 tggctggcaa agccgaaggt aaagccattg ccgatatggt aaaacagctt cttgcaggta
      781 attagtcctc cactggtcgg aacttatcag ttccctcaat acgctccagc tccgacccgg
      841 ttttcagttt caggtacgga attatagccg ggtcgcagtt ggcaatatag ttcaggtcga
      901 acatcatgaa gtggtaagga ttctcaacgt attcctcatc ttcggttccg gaataaaagc
      961 gccaaccgct atcctcggtt tcctcaggcc tttcacgatg catgtaaccc acaggctcac
     1021 catcaacggt tattttgtcc gaggcatagc agtaccttcc cgagggtatt aaatccttaa
     1081 cgtcttcagg tcttatttta tacttaaact cccccatgtt ttaccattat taaattaaaa
     1141 agtacaatgc aaaaaagtga cacgcactgc caagtattat aaaaaggtga aatataccgt
     1201 gaacaaaagc atagtgatct ttaaggtaga aaataacgct tgagctgtag gctaaacaac
     1261 ctgatagtag aaaccataac gataggttgg gagtgttttt aataatagga acaacggcaa
     1321 ttaatccaat ccaccccatt gcaatgtaaa gccatgccga tacgcttcgg tactttacgg
     1381 agtaaaacca tagcttaaac accactcctg ctacagccat tccccaaacc agcccaaaaa
     1441 cagtccaacc caaccaaccc tttatgctca gcaaagcaaa gggggtatag gttgctgcta
     1501 ttaaaatata gattgaagaa tgatcaaact tgtttaggtt gaacttgacc cgagggttaa
     1561 tagcggaatg gaaaagtgtt gatgctgtgt aaaggtttat taatccggct ccaaatattg
     1621 agtaagtaac aatatgccag gctgtgccat gtaaagcagc tcgtataacc agtagaactg
     1681 tggcagcaat agcaagcact ataccaaccc catggcttgt gctattaaca atctcctcat
     1741 actttttaat tcttttcctt gacgaattca tacaaaatgc ttttaaaacc tcataaaggt
     1801 agttaaaatt ggggtttgtt aaagctaatt actatttcat ttggttaagg atgctgaata
     1861 ttctatggcc agaaagcctg aaacgcttta ctgtgcccta aacccctatg ctttacttta
     1921 taatcaaaat tcagcatgaa agtttaatgc ttttaacttt atccattaaa ccttgtcctt
     1981 tatcctttat cctttaaact ttatccttta aactttatcc tctccccctc ccctatccgt
     2041 aattcacaac ttttcagtat ctttgcagtt caattgaata gaataggatg gagaatatca
     2101 ggaacttttg cattattgcc cacattgatc atgggaaaag taccctggcc gacaggctac
     2161 tggagttaac caatacccta tcggaccgcg agttacagga acaggtgctg gacagcatgg
     2221 acttggagcg agagaagggc attaccatta aaagccacgc catacaaatg gagtacactt
     2281 ttcagggcaa acagtataag cttaacctga ttgatacacc gggacacgta gatttttcgt
     2341 acgaagtatc gcgagccata gcctcatgcg agggcgcact gctggtggtt gatgccactc
     2401 agggcataca agcccaaacc atatcgaacc tctaccttgc acttaaccac gacctggaaa
     2461 taatcccaat tatcaacaag atagatatgg atgcagccat ggtggacgat gtaaaggatc
     2521 agattgtaga tttgcttggc tgcaaacgcg atgagataat ccctgttagt gcaaaaaccg
     2581 gcgaaggtgt ggacaaagta cttgaggcta ttgttacccg tgttaaaccg cccgttggcg
     2641 acccaaatgc acccttgcaa gccctgatat tcgactcagt tttcaactcg ttccgcggca
     2701 ttattgccta ctacaaagta tttaacggaa ctattcgaaa aggcgataag gtaaaatttt
     2761 tcaatacagg acgggagtac gaggccgatg aggtaggtat tcttcgatta aagttacagc
     2821 cccgcgatta cattagcgcc ggcgatgtgg gttacatcat atcgggcatt aaggaatcga
     2881 atgaggttaa ggtgggcgat accattaccc atgtggagcg cccatgcgat aaggctattg
     2941 ccggttttga ggatgttaaa cctatgctgt ttgcaggcct ttacccggtt gatgccgatg
     3001 agtatgagga tctcagagcc tcgcttgaaa agctgaaact taacgatgcc tccatcacct
     3061 ttgagcccga atcgtcgctt gcactaggct ttgggttccg atgcggattc ctggggctgc
     3121 tgcacatgga aattgtgcag gaacgccttt accgcgagtt cgacatggat gtaattacca
     3181 ccgtgccaaa cgtttcgtac cttgttcaca caacaaaggg agaggttgtt gaggttcata
     3241 accccagcgg cttacctgcc caaaccacca tcgattacat tgaggaaccc gtaatttcgg
     3301 ctcaaattat tacaaaatcg gaataccttg gcgctgtgat gaagctctgc atcgataagc
     3361 gtggaattct gaaaaatcag gtattcctta cctccgacag ggttgaactt tcgttccggt
     3421 tacccctgag cgagattgtg tttgactttt acgataagct caagtccatt tcacgtggtt
     3481 acgcctcgtt cgattacgtg atggatggtt accaggaagc aaagcttgta aaactcgata
     3541 tactccttaa cggcgaaatg gtcgacgcac tttcgtcgct aatccaccag gatcatgcct
     3601 acgattttgg ccgcaagatg tgcgagaaac taaaagaact tattccccgc cagcagttcg
     3661 atattgccat ccaggctgcc ataggctcaa agattattgc acgcgaaact gtaaaggcgc
     3721 ttcgcaagga tgtaaccgca aagtgttatg gggg
//
LOCUS       DAOOWJ010000172         3739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_24668_length_3739_cov_6.837676, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000172 DAOOWJ010000000
VERSION     DAOOWJ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24668_length_3739_cov_6.837676"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1182
                     /locus_tag="PLW22_11515"
     CDS             79..1182
                     /locus_tag="PLW22_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOU75372.1"
                     /translation="MAQKFIPFEVIENFMVSVLVKAGIPENDAKIVTDVLIQADKLGF
                     DSHGVNRLKPIYLDRIKDGILNPVTNYQIVKEGPTTAVIDGQNGMGHVIGYNAMKMAI
                     EKAKKFGMGMVAVRNSTHYGFAGYYALMAVKENLIGITGTNARPSIAPTFGVENMLGT
                     NPLTFGMPTDEPFPFLLDCATSITQRGKIELYEREGKILPKGWVIDENGESKTDPKEV
                     LVDLTTGKAALTPLGGIGEETAGYKGYGYATVVEILSSALQQGAYLKMLTGFKDGKKV
                     PYNLGHFFIAIDISAFTEVDDFKRTAGNILRELRASKKMPGQPRIYTAGEKEYEVWKT
                     RKDKGVPFGDELLKEFQGICKEYGLNEFLPSFE"
     gene            complement(1390..>3739)
                     /locus_tag="PLW22_11520"
     CDS             complement(1390..>3739)
                     /locus_tag="PLW22_11520"
                     /inference="COORDINATES: protein motif:HMM:NF027725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5018 domain-containing protein"
                     /protein_id="HOU75373.1"
                     /translation="VYYSFILKVTDLIAATEALGGYFIGFAYDNTNFGATVWTKRVDD
                     NSYLIGINSRTNTSATKFTTDSYSKDAEVFIVVSYEVVDGTGNDVAKIWINPASQDFG
                     AASEPASTLSITNTDGTDLTSVKRIFIRQDSNTETPGIELDELRVGDTWAQVTPKGAL
                     SSNATVTSTVYTVDSNAGTITNVPQTETLANFKANITPAAGATFEVYQADGTTVATDL
                     QTGYKLICTAEDGVTKKTYTITLNTSLNSEKAILSYSINGISGTIDAVNHTVTLTLPY
                     GTDRSNLVATFTLSAAATAKVGATVQESGVTANDFTNPVTYTVTAEDGSTQDWVVTVN
                     LAVASTEKDILTFTIAGQVSSVIDANAATVNVVMPFGTNVTSLTPTITVSQYANIEPA
                     SGVAQDFTNPVVYTVTAQDGSTKQYTVTVTVQDIPLTTIYSIQYTTDASGDSPLKTMQ
                     VRTKGVVTAVKSGSSTFNMWLQDSAKAWNGVYVYGVNNSFGTVAQGDSVEVIGTVDEY
                     NNLTEIKSVSYLNKINSSNTLPTPVDVTPEQASSEAYEGVLVKLNNVECTVADAGFGE
                     FTVSDGTNTINVDDFLYKYTPTQGSRYNITGVVDYSFGAFKVLPRSANDVESAQVKYT
                     VTFTVKRSDGTTPVDGATINVTGQGSVTTDASGVATMQLPDGEYTYTVVKTGFDTNNG
                     SFTVNGADLPVNITLIQTGIPANPIAKLSVYPNPFTDRIYFTGAEVTRVTITSVIGQV
                     VVDRQVENTESVDVSSLDRGIYLVRFYNSKGESVLRKLVKEE"
BASE COUNT         1031 a          793 c          911 g         1004 t
ORIGIN      
        1 gataaggaaa tttttgtttt gtggcaggaa tttaaattcc gtaattttgg attctgaaaa
       61 gcaaaaaaac taaaacctat ggcgcaaaaa tttattccct ttgaagttat tgaaaacttc
      121 atggttagtg tactggtaaa agccggaata cctgaaaacg atgcaaaaat tgtaaccgat
      181 gttttaattc aggccgataa gcttgggttc gattcacacg gggtaaacag gctaaaaccc
      241 atttacctcg atcgaataaa ggacggtatt ctgaaccccg ttaccaacta tcaaattgta
      301 aaggagggcc caaccactgc cgtaattgat ggccagaatg gcatggggca tgtaattggc
      361 tacaatgcca tgaaaatggc aattgagaag gccaaaaagt ttggaatggg aatggttgcc
      421 gtacgtaact caactcacta cggttttgca ggctattacg ctttaatggc agtaaaggag
      481 aatctgattg gtattaccgg aaccaatgct cgcccatcaa tagcacctac ctttggggtg
      541 gagaatatgt taggcacaaa cccattaaca ttcggaatgc ctaccgatga gcctttcccc
      601 ttccttctgg attgcgccac atcaattacc cagcgtggta aaattgagct ctacgaaagg
      661 gaaggtaaaa tattgcccaa gggttgggtg attgacgaga atggcgaaag caaaaccgat
      721 cccaaggaag tactcgtcga tttaacaact ggcaaggcag cattaacgcc acttggaggt
      781 attggcgagg aaaccgcagg ttacaaggga tatggttatg ccactgttgt ggaaatcctt
      841 tcgtcagcac tacagcaggg agcttacctg aaaatgctca ccggctttaa ggatggcaaa
      901 aaggttccat acaacctggg tcatttcttt attgctattg atattagtgc gtttactgag
      961 gttgatgatt ttaagcggac tgctggtaat atcctgcgtg agctaagggc ctcgaaaaag
     1021 atgccgggac aaccccgtat ttataccgct ggcgaaaaag agtatgaggt ttggaaaacc
     1081 cgtaaggata aaggcgttcc gtttggcgat gaactcctta aggagtttca gggaatttgc
     1141 aaggagtatg ggttgaacga gtttttgccc agctttgagt aaaggaaagg ataaaggata
     1201 aaggataaag gataaagggt aaagaataaa aatttaaaat ttcagatttc agattcatga
     1261 tagaaggtgg tttcttaagc cgtcaaccat caaccatcaa ccatcaacta taaaccatca
     1321 actataaacc aacaaccaaa aagaaacccc gcattagcgg ggttctttcg ttttattgca
     1381 atgggaatgt tattcctctt ttacaagctt gcgtagtacc gattcgccct tgctgttgta
     1441 gaagcgtacc aggtaaatac cgcggtcaag tgagctaaca tcaaccgatt cagtattctc
     1501 aacctgacga tccactacta cctgaccaat taccgaggtg atggttacac gggtaacctc
     1561 agcgccggta aagtagatac gatcagtgaa cgggttgggg taaacgctta gcttagcaat
     1621 ggggttagca gggatacctg tttgaatcag agtgatgtta acaggcagat cggctccgtt
     1681 tacggtaaag ctaccgttat tggtatcaaa acctgtcttg actaccgtgt aggtgtattc
     1741 accatcgggg agttgcatgg ttgccactcc gctggcatcg gtagttacgc tgccctgacc
     1801 tgtaacgtta atggtagcac catcaacagg ggttgtacca tcgctgcgtt taacggtgaa
     1861 ggtaacggtg tacttaacct gagcgctttc cacgtcgttg gcactgcgcg gtagaacctt
     1921 gaatgcgcca aagctgtagt caaccacacc ggtaatgttg taacgtgaac cctgggttgg
     1981 tgtgtacttg tacaggaagt catctacatt aatagtgttg gtaccatcgc ttacggtgaa
     2041 ctcgccaaaa ccggcatcgg caacggtgca ctcaacattg ttgagcttta ccagaacgcc
     2101 ctcgtatgcc tcgctggaag cctgttcggg tgtaacatca actggggttg gcaatgtatt
     2161 actgctatta atcttattca ggtagcttac gctcttaatc tcggtaagat tattgtactc
     2221 atcaacagta ccaataactt caacgctatc gccctgagca actgttccaa aggagttgtt
     2281 tacaccgtaa acatacacgc cattccaggc tttagcgcta tcctgcaacc acatgttgaa
     2341 ggtggatgaa ccagatttaa cggcagttac aacacccttt gtacgaacct gcattgtctt
     2401 gagcggtgaa tcgccactgg catcggtggt gtactggatg ctgtaaatcg tggtaagcgg
     2461 tatatcctga acggttacgg ttacagtgta ctgcttggtg ctaccatcct gagcggttac
     2521 ggtgtaaacc acggggttag tgaagtcctg agccacgccg ctggccggtt cgatgttggc
     2581 atactgcgat acggtaatgg taggtgttaa gctggtaaca ttggtaccga atggcattac
     2641 cacattaaca gtggcagcat tggcatcaat aaccgacgaa acctgaccgg caatggtgaa
     2701 ggtaaggata tccttctcag tgcttgctac ggcaaggtta acggttacca cccaatcctg
     2761 tgttgaacca tcctcagcgg ttacggtgta ggtaacgggg ttggtaaagt cattggcagt
     2821 tacgccactc tcctgaacag ttgcaccaac cttagcggta gcagcagccg aaagggtgaa
     2881 tgtggcaacc aggttgctcc tatcggtgcc gtagggcaag gtaagggtta cagtgtgatt
     2941 aactgcatcg atagttccgc taatcccgtt gatactgtag ctgagaattg ctttctcact
     3001 gttcaacgaa gtattgaggg taatggtgta ggttttcttg gttacaccat cctcggcggt
     3061 gcagatcagc ttatagccgg tttgcagatc ggtggcaacg gtggtaccgt cggcctggta
     3121 aacctcaaag gtagcaccgg ctgcaggagt gatgttagcc ttaaagttag ccagcgtttc
     3181 ggtttgtgga acattggtaa tggttccggc attgctgtca acggtgtaaa ccgttgaggt
     3241 aacggtggcg ttgctgctta aagctccttt aggggtaacc tgtgcccatg tatcacctac
     3301 acgtagctca tcaagttcaa ttccgggagt ttcggtattg gagtcttgac gaatgaaaat
     3361 tctcttcact gaagtaagat cagttccgtc tgtattggta atacttaggg ttgatgctgg
     3421 ctcactggca gcaccaaagt cttgtgaagc aggatttatc caaattttgg ctacatcgtt
     3481 tcctgttcca tctacaacct cgtacgatac tacaatgaac acctctgcat cctttgagta
     3541 gctatcagtt gtaaactttg tagctgaagt gtttgttctg ctattaatac caatcaggta
     3601 actattatca tcaacacgtt tagtccaaac agttgcacca aagttagtat tgtcataggc
     3661 aaaacctata aaatatccac ctagcgcttc ggtcgcagca attaaatcgg ttactttaag
     3721 tataaatgag tagtaaaca
//
LOCUS       DAOOWJ010000173         3720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_24824_length_3720_cov_4.254025, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000173 DAOOWJ010000000
VERSION     DAOOWJ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24824_length_3720_cov_4.254025"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            740..1498
                     /locus_tag="PLW22_11525"
     CDS             740..1498
                     /locus_tag="PLW22_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOU75374.1"
                     /translation="MKKYLLILTILSVTVACNRKSQLVYLNDVTNETLGSVKAPVYLI
                     KPGDVLSIQIYTQDPNISRLFNIANTQTAYSAYNSEISTYITGFSVSDSGDVNLPVIG
                     KVKIAELSVRDAQEAIQQAADRYLKDALVVVKLLSFRVTVLGEVKRPGTYTNYKDNLN
                     IFEAIGLAGDLSDFGKRTNVLVLRNTPQGVKTFRMDLQDKNIVASDGFFLQPNDTVIV
                     EPRSGKVMALNTPNIQLLLSTLTTVLLFLNFLKK"
     gene            1555..>3720
                     /locus_tag="PLW22_11530"
     CDS             1555..>3720
                     /locus_tag="PLW22_11530"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOU75375.1"
                     /translation="MAQDSNMNQDLIPRNPAGYPEEETLDIKQLLYRVIANWYWYAIS
                     VFIALFVAYLVNRYSQQIYSVTSSVIVRDDDQTKSYVGAEQLIQSLRLVKNTKSVQNE
                     IGILQSYSLARQTIEELDEFNVTYVAVGRRGIKESKLYKRCPFKVITDSGAVNLINYP
                     VYVTILDSLRYTVEIDDGMGIKQTLLFGQPFNHENFNFTLQLRDYASYKDAQTVSKYY
                     FYFNDPNSLALQYKSKVTVTLNDKKGSILTLNSSGFVAQQEADYLNKLMEVYIRRGLE
                     EKNKIAENTVKFIDQQLALMTDSLRRAEQRLQDFRTTNRVIDISREGSMLIDRIKAIQ
                     QEQGAIELKGYYYGYLKEYLAQRKDLNTLIAPSVLGVDDPQLNTLLAEASKAYIEREE
                     LRASVKAGSPGLEQLDIRIENLRQALIEKVNSLIDANAIAQKQLKNRIKEVEAEMIRL
                     PVNERLLIGFERDFGLIDKMYTYLQEKRAEAAIARASNIPDNKILDYALAENATMIKP
                     KKQMNYLIALLAGLALPFIIMLIYDFFNDKLVDLKEISRRTRVPVIGTLGHNRYETEL
                     PAIEKPKSTLTESFRGLRTNLQYLLREPDKKVVSVSSTVVGEGKTFIAANLAAITAVT
                     GKKVLLVGLDLRKPRLHRLMGYTGTVGISTYLIGRSNIDDIVFPSQVDNLYYAPSGPI
                     PPNPAELIGSPKMEEFMAWARSNFDYVVVDSPPVAIVSDA"
BASE COUNT         1036 a          937 c          846 g          901 t
ORIGIN      
        1 ctattcgcca cgcctgtggc gtggctcatg tggtatcgct tactaagccc accgcaacgt
       61 tccacccccg acccattctc gggtctggct tttatgccac tgcttacgtc attccttgct
      121 ggttgaagaa tatcattaat aatagatgaa ggtgcaaaga gatgcttcgg ctaagctcag
      181 catgacaata gacaagcggg gcaactaaca accaacaact aaataggtat cacagggttt
      241 cgcagagttc acacagtgtt acacagtgaa aatctgcatt atctgtgtca tctgtgttcc
      301 attccatctc acgaacaacg aataacgaac aacattcaac caacccgacc cctctaaatc
      361 tcccccgaga ggggagactt ccatttttct gtaaggattt tgggctttcc cttctcctct
      421 caggagaggg gcaggggtga ggtcggttct ccactaacaa ccatcaacca tcaacgaaca
      481 actaactcat ctgcgtcatc tgcgttccat ttcatctcac gaacaacgaa taacgaacaa
      541 tattcaacca acccgacccc tctaaatctc ccccgagaga ggagacttcc atttttctgt
      601 aaggattttg ggctttcccc tctcctcaca ggagtggggc aggggtgagg tcagtttcca
      661 accaccaact ttcaattttt tccctatatc agactaaaca taatcacaaa atactatctt
      721 tgtatgataa acggttacga tgaaaaaata tctgcttata ctaaccattt tatcggttac
      781 ggtggcgtgc aaccgtaaat cgcaactggt ttaccttaac gatgttacca acgaaaccct
      841 gggttccgtt aaggctcccg tgtatctcat taagcctggc gatgttttga gcattcaaat
      901 ctacacccag gaccccaata tcagcaggtt atttaatatt gctaacactc aaaccgcata
      961 ttctgcttac aattccgaaa tatcaaccta tatcacaggg ttttcggtaa gcgattcggg
     1021 cgatgtgaat cttccggtta taggaaaggt aaaaatagca gagctctcag tgcgcgatgc
     1081 gcaggaggcc attcagcagg ctgccgaccg ttacctgaaa gatgccctgg tggtggttaa
     1141 gctgcttagc tttagggtaa ccgtgctggg cgaggtcaag cgccccggta catacaccaa
     1201 ctacaaggat aatctgaaca tctttgaggc cattggtttg gctggcgacc tctccgattt
     1261 tggcaaacgc accaacgtgc tggtgctgcg gaatacccct cagggggtaa aaacattccg
     1321 tatggatttg caggataaga atattgtggc atccgatggc tttttcctgc aacccaacga
     1381 taccgttatt gttgaacctc gtagcggaaa ggtgatggcg cttaacacac ccaatatcca
     1441 actgctgcta tcaacactaa caacggtttt gctatttttg aactttttaa agaaatagat
     1501 gttaaaacta cgattcatgt ttaacaatat taactaagag gagaaaggta gcgaatggca
     1561 caggatagca acatgaatca ggacctaatt ccccgaaacc ccgccggtta ccccgaggag
     1621 gaaaccctcg acataaagca actgctttac cgcgtaatag ccaactggta ctggtacgcc
     1681 atatcggttt tcattgcact ttttgtggca tacctggtta accgttactc gcaacagata
     1741 tacagcgtaa catccagcgt aattgtccgc gacgacgacc aaaccaaatc gtatgtggga
     1801 gctgagcagc ttatacaaag cttaaggctg gtaaaaaata ccaagagcgt ccagaacgag
     1861 ataggaatcc ttcagtcgta ctcccttgcc cgccaaacca ttgaagaact cgacgagttt
     1921 aatgtaactt acgtggcagt tggccgacgt ggtataaagg aatcgaaact ctacaaaagg
     1981 tgtcccttta aggtaataac cgacagcggt gcggttaacc tgattaacta tccggtttac
     2041 gtaaccattc tcgactcctt acgctataca gttgaaattg acgatggcat gggtattaag
     2101 caaaccttgc tttttggcca accattcaac cacgaaaact tcaactttac cctgcagctg
     2161 cgcgattatg ccagctacaa ggatgcacaa accgtaagca agtactactt ctactttaac
     2221 gaccccaaca gcttggccct gcagtataaa tcaaaggtaa cggtcacctt aaatgacaaa
     2281 aagggttcga tactaaccct gaatagcagc gggtttgtgg cccaacagga ggccgattac
     2341 ctgaacaagc tcatggaggt ttacatcagg cgtgggctgg aggaaaaaaa caagattgcc
     2401 gagaacacgg ttaaatttat tgaccagcaa ctcgctttga tgaccgattc gctccgccgg
     2461 gctgagcagc ggttgcagga tttccgcacc acaaaccggg taattgatat atcgcgcgag
     2521 ggcagcatgc taattgatcg catcaaggcc atacaacagg agcagggtgc cattgagcta
     2581 aagggttact actacggcta tttaaaggaa tatcttgccc agcgcaagga tttgaacacc
     2641 ctgatagccc cctcggtgct gggggttgac gatccacagt taaataccct gctggccgag
     2701 gcctcaaagg cctacattga gcgtgaggag ctcagggcat cggttaaagc gggtagcccg
     2761 ggattggaac agctcgatat ccgtattgaa aacctcaggc aagcgttaat tgaaaaggtt
     2821 aatagcctga ttgatgccaa tgccattgcc caaaagcagc tcaagaaccg cattaaggag
     2881 gtggaggccg agatgatccg cctgcctgtg aatgagcggc tgctcattgg gtttgagcgc
     2941 gattttgggc taattgataa gatgtacacc taccttcagg aaaagcgtgc cgaggcagcc
     3001 attgcccgcg cctcaaatat acccgataac aagatactcg actacgccct ggccgaaaat
     3061 gccaccatga ttaagcccaa gaagcagatg aactacctga ttgccctgct tgcaggcctg
     3121 gcattgcctt ttatcatcat gctgatatac gactttttca acgataagtt ggttgacctc
     3181 aaggagatat cgcgccgcac cagggtaccc gttattggaa cccttggcca taaccgctac
     3241 gaaactgaac tgccagctat agaaaaacca aaatcgaccc taaccgaatc gttcaggggg
     3301 ttaagaacta acttgcagta cctgctgcgt gagcccgata aaaaggtggt gtcggtatca
     3361 tcaaccgtgg tgggcgaggg caaaaccttt attgctgcaa accttgctgc cattacagcg
     3421 gttaccggta aaaaggtgct actggtaggg ctcgaccttc gtaaaccccg cctgcatagg
     3481 ctgatgggct ataccggcac agtgggtatc agcacttatt taataggtcg gagtaatatc
     3541 gacgatattg tattcccctc gcaggtcgat aacctttact atgcccccag cggacccatt
     3601 ccacccaatc cggcagagct gatcggttca cccaaaatgg aagagtttat ggcctgggcg
     3661 cgcagcaact tcgattacgt tgtggttgac agcccccctg tggccattgt ttccgatgcc
//
LOCUS       DAOOWJ010000174         3691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_25077_length_3691_cov_8.622112, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000174 DAOOWJ010000000
VERSION     DAOOWJ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25077_length_3691_cov_8.622112"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLW22_11535"
     CDS             complement(<1..584)
                     /locus_tag="PLW22_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU75376.1"
                     /translation="MKGIIAFFIPLLYAVNLFAQKPLNRLTYDEVLQLAKDQSPQAIL
                     AKHRFRAAYWQYRTYVAELRPNLTLTGTIPQFSRSLIRYQNPDGSYTYIEENSNTTSL
                     GLSLSQNIGLTGGQIFVRSNLERTDLLGSDKTTSYMSVPVSIGFSQPLFAMNQLKWSK
                     RIEPIKYEEAKRAFIQSMEAISMEATRLFFDLAMA"
     gene            complement(606..1976)
                     /locus_tag="PLW22_11540"
     CDS             complement(606..1976)
                     /locus_tag="PLW22_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU75377.1"
                     /translation="MFQIDRLYRRYSHDITIALEAIMANKLKSVLTALGIIFGVAAVI
                     SMLAIGNGAQQEILEQMKMVGVNNIIVNSIFEAPDSQSESENGNKQKQKFSPGLTLSD
                     LEAIQQVVPSVVHISPEINLNSYIQYNGLRMQAKILGVTNDYFALFNLPLDEGTYFSA
                     NQEEHGIPVCVIGANIKTKFFSTENPIGKYIKFGNVWLKVIGVLAKSDIKVTGFENSG
                     INVYNDNIYVPAKNILLRYQNRALVNSKKLNQSNTIRGFGFIIFGGSTETQAPKNYHQ
                     LDRIILQVKGTEDIASTSEILSRMLLRRHSGVKDYEITVPELLLKQQQRTKDIFNIVL
                     GAIASISLIVGGIGIMNIMFASVMERIKEIGTRLAIGAKKADIVAQFLAEAVLISVTG
                     GLIGVILGVILSKLITHFAGILTIVSFSSIIIAFGVSAAVGVIFGLSPAKRAAEKDPI
                     ESLRYE"
     gene            complement(1982..3442)
                     /locus_tag="PLW22_11545"
     CDS             complement(1982..3442)
                     /locus_tag="PLW22_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU75378.1"
                     /translation="MKRKYWFILIAIAVVVILLTAVLNSGSKDKAVPQFTRAEVGEFE
                     IVVNVTGELTAQNSEDITAPTELRGRSFRVTDVKIQDLVPEGTVVDSGDYVATLDRSA
                     LSNQLKNTEDELEKSQQAYLKTQLDTTLQLRELRNNLINLKFDMEEKKLILEQSKYEP
                     PATQRQAQINLEKAERAYQQALYNYNLKKDQAEASMKEVSINLERQRRERQDMLDVIS
                     KFVIKAPKQGMVIYYREWSGQKRKVGSTVSPWDLTVATLPDLSVMNSKTYVNEIDISK
                     VKKGQPVRITVDAFPDKKFTGEVIEVANIGEQLPNTDAKVFEVTIRVNEYDPILRPSM
                     TTGNQIVTNTYDSVLFIPLEALHANDSISFVYTRKGKRQVVVVGDMNENFIIVEKGLK
                     PGEELYLSTPEKPEKFEFTGFELIPEIKKRKEDKLRQEAELRQQQQELMNKRKNGFGM
                     PGQGNVMIISTDKPADNKNSSSAPKNTAKDQMKTKP"
BASE COUNT          955 a          773 c          791 g         1172 t
ORIGIN      
        1 gccatggcaa ggtcaaagaa tagtcgcgtg gcttccattg atatggcctc catgctttgg
       61 ataaaggcac gtttggcttc ctcgtactta attggctcaa tgcgcttgct ccacttaagc
      121 tgattcatag cgaaaagcgg ctgggagaaa ccaatggaaa caggtacgct catgtaggag
      181 gtggttttat cggagcccag taaatcggta cgttccaggt tgcttcgtac aaaaatttga
      241 ccaccggtta agccaatatt ttgtgagagc gataatccta gcgaggtggt atttgagttt
      301 tcctcaatgt aggtatatga cccgtcaggg ttctggtaac gtataagcga acggctgaac
      361 tggggtattg ttcccgtaag ggtaaggttt ggcctaagtt ctgcaacgta ggttcggtat
      421 tgccagtagg ctgccctaaa ccgatgcttg gccagtatgg cttgtggcga ttgatccttg
      481 gcaagctgta acacctcatc gtatgtaagc ctgtttaatg gtttttgggc aaaaaggttt
      541 actgcgtaaa gcaatgggat aaaaaatgca attattcctt tcatatcaac ttttttatgg
      601 taacattatt cgtaacgtaa cgattcaatt ggatctttct ctgcagccct tttggcaggc
      661 gataggccaa atattacacc taccgcagcc gatactccaa atgcaattat tattgatgag
      721 aagcttacaa tggtaagtat tccggcaaaa tgcgtaatca gcttggatag gataactcca
      781 agtatgacac caatcaaacc tcctgtaaca cttataagca ctgcctcggc aaggaattgg
      841 gcaacaatat cggctttttt agcaccaatg gctaaacggg ttccgatttc cttgatgcgt
      901 tccatgaccg atgcaaacat gatattcatt attccaatgc cacctactat aagtgagatg
      961 cttgcaatag cccccagaac aatattgaag atatccttgg tacgctgttg ctgctttagc
     1021 agcagttcgg gcacggtaat ctcgtaatcc tttaccccgg aatgcctgcg tagtaacatt
     1081 ctgctaagta tctccgaagt tgatgcaata tcctcagtgc ccttaacctg tagtataatc
     1141 ctatcgagct gatggtagtt tttgggggct tgtgtttcag tacttccacc gaaaattata
     1201 aatccaaacc cacgtatggt gttactttga tttaacttct tagagtttac caaagcccgg
     1261 ttttggtatc gcagcaaaat gtttttggct ggaacgtaga tattatcgtt ataaacgttt
     1321 ataccgctat tttcgaaccc agtcactttt atatccgatt tggcgaggac tccaatgacc
     1381 tttagccaaa cattcccaaa cttgatgtat tttccaattg gattctcggt actgaaaaat
     1441 tttgttttga tgtttgcccc tataacgcaa accgggattc catgttcttc ctgattggca
     1501 ctaaagtagg ttccctcatc gagcggcagg ttaaaaagag caaagtagtc gttggttacg
     1561 ccaagtattt ttgcctgcat ccttagtcca ttgtactgga tatatgaatt taaattgatt
     1621 tcagggctga tgtgaaccac tgagggaact acctgctgga ttgcctctaa atcggaaagt
     1681 gtgaggccgg gggagaattt ttgtttctgc ttatttccgt tctccgattc cgattgggaa
     1741 tcaggagcct caaaaatgga gtttactatg atattgttaa ccccaaccat tttcatttgc
     1801 tccagtatct cctgttgagc accattacct atggccagca tgctgataac cgcagcaacc
     1861 ccaaaaataa ttccgagtgc ggtaagtacc gattttagct tgttggccat aatagcctca
     1921 agggcaatgg ttatatcgtg tgagtatcgc cggtatagcc tgtcgatttg aaacatagct
     1981 gttagggttt tgttttcatt tgatcttttg cagtgttttt aggtgcactg ctgctatttt
     2041 tattatcggc aggtttatca gtggaaataa tcataacatt cccttgtccg ggcataccga
     2101 aaccgttttt acgcttattc ataagctctt gctgctgctg gcgtaactca gcctcctgtc
     2161 gcagcttatc ctctttcctt tttttgattt cagggattag ctcaaaaccg gtgaactcaa
     2221 atttttcggg tttttctggt gttgaaaggt aaagctcttc gccaggttta agcccctttt
     2281 caacaatgat gaaattctcg ttcatatcgc ccaccacaac aacttgacgt ttacccttac
     2341 gggtgtaaac aaagctaatg ctatcgttag cgtgaagagc ctcaagtggt atgaaaagaa
     2401 ccgaatcgta agtgttagta acaatctggt tgcccgtagt cattgatggg cggagaatgg
     2461 gatcgtattc atttacccta attgtcactt caaacacttt tgcatcggta ttgggtagtt
     2521 gttcgccaat gttggcaacc tcaatcacct cgcctgtgaa ttttttatcg gggaaggcat
     2581 ctacagtaat tctaaccggt tgccctttct ttaccttact tatgtcaatt tcattgacat
     2641 acgttttgga gttcatcact gagaggtcgg gcaacgttgc aacggtaaga tcccaggggc
     2701 ttacggttga accaacctta cgcttttgtc cgctccattc gcggtagtat atcaccatgc
     2761 cctgtttggg tgctttaata acaaatttac tgataacatc gagcatatcc tgccgttcgc
     2821 ggcgctggcg ttcaaggttt atggaaacct ccttcatgga ggcctcggct tgatctttct
     2881 tcaggttgta gttatatagg gcttgctggt atgccctttc agccttctct aggttgattt
     2941 gtgcttgacg ctgtgtggct ggaggctcat actttgactg ctccagaata agttttttct
     3001 cctccatatc gaacttcagg ttaataagat tattccttag ttcccggagc tggagcgtgg
     3061 tgtcgagctg ggtttttagg taggcttgtt ggctcttttc cagctcatct tcggtattct
     3121 tgagctggtt gctcaaagct gatcggtcaa gggtggccac gtaatcgccg gagtccacaa
     3181 cagtcccttc agggactagg tcctggattt ttacatcggt aactcggaac gatcggccac
     3241 gcaactcggt tggtgctgta atgtcctcag agttctgtgc cgtaagttca cctgtaacgt
     3301 taaccactat ttcaaactct ccaacctcgg cacgggtgaa ctggggaact gctttgtcct
     3361 ttgaacctga gtttaaaact gctgtaagca aaataacaac aactgcaatt gcaattaaaa
     3421 taaaccagta tttacgtttc attgtttggt taggtttgtt tatacaaaag aatgaaaaac
     3481 ttaacattgg tttaaacggt aaatgttaaa taatatttca aagatcaaaa atctatttga
     3541 acccatttta taaagattat ttggttaatg tgtgttaaaa acaaaattgt cgtgtcaaaa
     3601 aaaattgaat tataccattt tatagtatta actttattta aagaatttct cattctttaa
     3661 tcaaaactaa tattaaaatc aaatcaagat g
//
LOCUS       DAOOWJ010000175         3654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_25401_length_3654_cov_5.674632, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000175 DAOOWJ010000000
VERSION     DAOOWJ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25401_length_3654_cov_5.674632"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLW22_11550"
     CDS             complement(<1..562)
                     /locus_tag="PLW22_11550"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU75379.1"
                     /translation="MRITGTVTSVDGTWPLQGVTVVVVGSTTGTVTNMEGRYEISVPA
                     IAKKLRFSYIGYKTVEVEIANRTVIDVEMEEEAIEVKEVTVTALGIMRAEKATGYAIQ
                     SVKGEELARTPELNIVNSLQGRISGAIITNTSGAVGASTRIVLRGVNSLSTDNQPLFV
                     VDGVVLNNTNFGGTYTEGVNRGSGIAD"
     gene            complement(1558..>1793)
                     /locus_tag="PLW22_11555"
     CDS             complement(1558..>1793)
                     /locus_tag="PLW22_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOU75380.1"
                     /translation="TQKYIALYEHEAIEAYNDYRRTGIPAMHNPNNTTVGFVNRFPWA
                     LSEVSSNQNVPQTELNYIYTTKVWWAGGDELVP"
     assembly_gap    1794..1893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1894..2639)
                     /locus_tag="PLW22_11560"
     CDS             complement(<1894..2639)
                     /locus_tag="PLW22_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOU75381.1"
                     /translation="MKINRILLLAAFTPFLFSCSEDVMDDINKEKNETTEMSADYIIT
                     DCGLKTAFETTGTDIAWYSSVYIEHSAGTWGQHASADTRTGQTSSSLMNNNWNSLYDV
                     LKNLKFVLDKTDPINGTEPNNRWARGIAYVLTAYNLAVATDMWGEVPWTEALQGTVIM
                     QPKYDKQSDIYPKIFAMLDSAIANLNLATTGLPSVDYIYGGTFANNKASWIRAANSLK
                     ARYWMRLSNRNTNAAANALAAINAGAFTSAA"
     gene            complement(2660..>3654)
                     /locus_tag="PLW22_11565"
     CDS             complement(2660..>3654)
                     /locus_tag="PLW22_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU75382.1"
                     /translation="ELKFFKNRLGIDYNYFDQTARNQIFTVPLAGSTGYSGAVMNAGE
                     MSSKGHEIVLNATPVQMKNFEWNLQANFTKIVNKCVELAPGVEDIFLGGYVTPNIRAS
                     AGDTYPAIYGERFLRDDQGRILVDDDPTSYTYGMPIVGEFGKIGEVSPDFIVGFNTSF
                     TIFKNFTLSAQIDWKQGGQMYSGSNRLMDLYGSSKKTEEWRLDPDGFIWPGYKSDGTP
                     NDIVRGGPNDVWAYPDLFNDVLGDLSEAHIYETSFVKLREVALGITLPEKWVNPILVK
                     RATVSFVARNILLWTTLPNFDPETSQGQGNQQGGFDYMSLPQTTSYGFTLNLTF"
BASE COUNT          947 a          850 c          719 g         1038 t
ORIGIN      
        1 tgtcggcaat accgcttccg cggtttaccc cttcggtgta agtaccacca aagtttgtat
       61 tgttgagcac taccccatca accacaaaaa ggggttggtt atcggtactt agcgagttaa
      121 ccccacgaag cacgatacgc gttgaagccc caactgcacc agatgtgttg gtaatgattg
      181 cgccggaaat tcgtccctgc aatgaattta ctatattcag ctcaggagtg cgagctaact
      241 cttcgccttt tacactctga atggcatacc ccgttgcttt ttcagcccgc atgataccca
      301 acgctgtaac cgtaacctct ttcacttcaa tggcttcctc ctccatttcc acatctatta
      361 cagtacggtt ggctatttca acctccactg ttttgtaacc aatgtagcta aagcgaagct
      421 ttttagcaat ggcaggaaca ctaatctcat atcgcccttc catgttggta accgtaccag
      481 tagtgctacc aacaacaacc acagtaactc cctgcaaagg ccatgtacca tcgaccgaag
      541 ttactgttcc cgtaattctc acagtttgtt gggcaaacac gttcaaagaa ctaacaataa
      601 aaaaggctgt gagaaaggca cacagtttcc tcataatact ctaattaaca gtttggttta
      661 ggtttaacac aatttcacca accaaattta gggtaaatag caatacgagt caaaaaaaat
      721 ttcccttccc ccccccaatt aagtaggaga caaaggtaaa cgtaaaattt acctttgtcg
      781 tcctctcaca ccaccgtatg tacttacgtg tacggcggtt tccttaaagt gggttacgac
      841 acaagtgtcg tctgatttcg ttcttcctcg cacatagaaa gccactcggg gtttaccttt
      901 ccgctgtttt gataccatac gggtatctcc accgcgaaga ggtcatcgct ataccaacat
      961 gctccctgtc ttgcagggtt catctgggtt tccgattatt tccttccctt ttccattggc
     1021 ctttactatc ctaaagaaaa actcctctgc tttaaggatt cgacccttcg ctccatttcc
     1081 attacagaaa cttcttcgct actatggtct ctgctgactt ctgtgtaacg gctttgcatt
     1141 accggttcgc ttgggatagc ctccgctggg ttacacagac ctcctacggt aaactcatta
     1201 cactttcgca atagctgtca tcatttacta cccttgttta cgttcaacta tcgggctttg
     1261 caatccaatg ctcgctaacc cacaaggtaa gccttgtatg atgttcctgt ccgtacagtc
     1321 attgctttgt cgctccgatt ttcatcggtc ttcttttggc ttccttcaga tttcgcctca
     1381 cggcaaacac ccgttcggct gagctcacgg ccgaagcctt gcctttgact aacacttccc
     1441 gttgactggt gtgttcgggg ctttcaccct atagtgtaat ggtatgcgta gcgcacaaaa
     1501 aaaacgaggg cgcaaaaaaa ggcgccctca tttaaattaa tagaaaataa atctatacta
     1561 gggaactaat tcatctccac cagcccacca aacctttgtt gtatagatat agtttaactc
     1621 ggtttgagga acgttttggt tggaactaac ctcacttaaa gcccatggga aacgatttac
     1681 gaagccaaca gtagtattat tggggttatg cattgcagga atacctgtac gacgatagtc
     1741 gttgtatgcc tcaatggctt cgtgctcata aagggctatg tatttttgtg ttannnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngctgcgg aggtaaaagc acctgcatta
     1921 attgcagcta atgcattggc tgcagcatta gtattacgat tgcttaaacg catccagtaa
     1981 cgggccttta atgagtttgc tgctcgaatc caagaagctt tattgtttgc aaatgttcca
     2041 ccataaatgt aatcaaccga aggtaatcct gttgtagcca ggttaaggtt tgctattgca
     2101 ctatcaagca tggcgaatat tttcggataa atatcgcttt gcttatcgta cttgggctgc
     2161 atgattacag ttccttgtaa agcctcagtc cagggtactt caccccacat gtcggttgca
     2221 acagcaaggt tgtaagcagt aagtacgtat gctatacccc ttgcccaacg gttgttgggt
     2281 tcggttccgt taataggatc agttttatca agtacaaact ttagattttt aagtacatcg
     2341 taaaggctat tccagttgtt attcatcaac gagctagatg tttgcccagt acgtgtatcg
     2401 gcagaggcat gttggcccca agttcccgca ctatgctcaa tgtaaaccga tgaataccat
     2461 gcaatatcgg taccagtagt ctcaaaagct gtcttcaggc cacaatcggt tatgatgtag
     2521 tcagcggaca tctcggtggt ttcgtttttt tccttgttga tatcatccat cacatcctcg
     2581 ctacagctga agaggaatgg tgtgaatgcc gccagtaaaa gtattctatt tattttcata
     2641 atgcttcaat ttttttacgt taaaaggtta agttaagagt gaagccgtaa gaggtggttt
     2701 gtggcagcga catgtagtcg aaaccaccct gctggttacc ctgaccctgc gatgtttcgg
     2761 gatcgaaatt gggaagggtt gtccagagta aaatattacg ggccacgaag ctaacggtag
     2821 cacgtttaac aagaatagga ttaacccact tttcaggtaa agtaataccc aaagctacct
     2881 cacggagttt tacaaatgaa gtttcataga tatgggcctc actgagatcg cccaatacat
     2941 cattaaatag atcaggatat gcccatacat cgtttggacc accacgaact atatcgttgg
     3001 gagttccatc gctcttatag ccaggccaaa tgaatccgtc ggggtcgagg cgccactctt
     3061 cggttttctt agacgaaccg taaaggtcca ttaaacggtt tgaacctgag tacatttgcc
     3121 caccctgttt ccaatcgatc tgggcgctga gggtaaagtt cttaaaaatt gtgaagctgg
     3181 tgttgaaccc tacaatgaaa tcgggtgaaa cttcacctat ctttccaaac tcccctacaa
     3241 taggcatgcc ataggtgtaa ctggtaggat catcatccac taaaatcctt ccctgatcgt
     3301 cgcggaggaa acgctcaccg taaatggcag ggtaggtatc gcctgctgat gcacgaatat
     3361 ttggagtaac atagccacca aggaagatat cctcaacacc cggagctagc tcaacacact
     3421 tgttaacaat cttggtaaag ttagcttgca ggttccattc aaagtttttc atttgaactg
     3481 gtgtagcatt tagcacaatt tcatgacctt ttgatgacat ttcaccagca ttcattacag
     3541 cgccactata acctgttgat cctgcaagag gtactgtaaa tatctggtta cgtgctgttt
     3601 gatcaaagta gttgtagtca atgcctaacc tattcttgaa gaacttcaac tcaa
//
LOCUS       DAOOWJ010000176         3635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_25596_length_3635_cov_9.308380, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000176 DAOOWJ010000000
VERSION     DAOOWJ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25596_length_3635_cov_9.308380"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLW22_11570"
     CDS             <1..768
                     /locus_tag="PLW22_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU75383.1"
                     /translation="RPVARVYDKYLYESDLQNIFLTNVSKEDSILIAKAYIEKWIQTQ
                     LLLKQAENNLTDKEKNVARQLEDYRASLLIHKYEQAYVNQKLDTLVKETEVEQYYYSN
                     KDNFVLSEPIVKALFIKLRKEAPQTPKIKEIYRSKKQEDIQLLDNLAYQAAIFYDFFN
                     DQWIPFSVITRQLPYTIDNIKETFSRQYIEMEDGSFIYLVSLREVKEQGETAPLAYIT
                     NEIKQLIINARKQNLIDQLERNLFIKAKDDRNFEIYK"
     gene            776..2125
                     /locus_tag="PLW22_11575"
     CDS             776..2125
                     /locus_tag="PLW22_11575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU75384.1"
                     /translation="MRAFSLPTLFLLIFSFSFSSHAQENISIDKVIAVVGNERILLSD
                     VEQELLRIKMQGSTNANIDRCQLLEQLLIQKLLITQAKIDSIEVNEGSVESEVDRRLK
                     YFINQIGSEKALENYFNKPIFEIKDDLREIIREQQLTQQMQRKIVDKVKITPSDVKAF
                     YKKIPSDSLPDIPMQYELQQIAIYPPDAEKAKLEAKEKLLEIRNRILNGERFSTLAAL
                     YSEDRASAIKGGELGFRSREELVKAFADAAFNLKEGQISQVVETEYGLHIIQMIERRN
                     DQVNVRHILLKPQFTTDLLSKAVQKLDSIATLIRNDSITFENAALRFSEDKKSNLNKG
                     LMINPFTNTSLFEKEHLQPSDFYAIKDLKEGEYSAPFESRDEHANIVFKIIKVKRIVP
                     AHKANLKDDYDKIQEMAKLSKENEILRDWVSKKIKSFYVRIDNDFHGCNFQSKGWIK"
     gene            2137..>3635
                     /locus_tag="PLW22_11580"
     CDS             2137..>3635
                     /locus_tag="PLW22_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOU75385.1"
                     /translation="MKSVLRIFFIVTILLGLHTGAHAQQKSRIRFEGAKSMKNLNING
                     QKIIRYIGNVRFRHENTRIVCDSAYQNPDDNWFDAFGNVSIQQGSTRITGQTLHFDGK
                     TSVGKITGKEVKLIDEETTLTTQTLFFNSKTNVAYYTTPGVITSKESKLSSQRGYFNR
                     FSNLLSFAGNVVMVDPNGTIYTDSLSYNTQNELAYFFGPTHIFNEDNYAYCEKGWYNK
                     KIEQTNLQQNAYILSQENKIFGNNIFYDKSIKFAHAIGNVVLVDTLNRAYIYGQKANF
                     WQETEQAEVTENPYLMLIDNSDSLFLKANYFKVITQKHENSTDSSYRVLKALGNVAFY
                     RHDVQGFCDSMIYNTRDSAIDMYVDPILWNDVNQMTADHITIHSKNNKINQMDFNGNA
                     FITSFEQDKFYNQIKGKTITAFFSNGKAEKIDVNGNGQAVYFIRDGNTLTLANRSESA
                     RISIMVKDNKASRVTFHEKPSSNLYPIEKVELEDVMLKGFKWLEAKRPA"
BASE COUNT         1247 a          768 c          703 g          917 t
ORIGIN      
        1 cggcctgtag caagggtata cgataagtac ctatacgaaa gcgaccttca aaatattttc
       61 ctcacaaatg taagtaagga ggatagtata ctaattgcta aggcttacat tgagaagtgg
      121 attcaaactc aactcctgct aaaacaggcc gagaacaacc ttaccgataa ggagaaaaac
      181 gtggcacgtc agctggaaga ttatagggca tcactactaa tacacaagta cgaacaggct
      241 tacgtaaacc aaaaactcga tacactggta aaggaaacag aggtagagca gtactactac
      301 tccaacaagg ataactttgt tctctcagag ccaatagtta aagcactatt cataaagctc
      361 cgcaaggaag cccctcaaac ccccaagatc aaggaaatat accgttctaa gaaacaggag
      421 gacatacaac tactggataa tttggcctat caggcggcca ttttctacga ttttttcaat
      481 gaccaatgga taccattctc ggtaattacc cgtcagcttc cttacacaat tgataacata
      541 aaggagacct tttcaagaca atacattgaa atggaagacg gcagtttcat ctacctggtt
      601 tcgctgcgtg aggttaaaga gcaaggcgaa actgcacctc tagcttacat aacaaacgag
      661 ataaaacagc taattattaa cgcgcgaaag caaaacctga ttgaccagct ggaacgcaat
      721 ttgtttatta aagcaaaaga cgatcgtaat tttgaaatct acaaatagta caaccatgag
      781 agcatttagt ttacctacac tttttttatt gattttttcg tttagtttta gttcgcacgc
      841 gcaggaaaat atctctatcg acaaggtaat tgcagttgtt ggaaatgagc gcatacttct
      901 atccgatgtt gagcaggaac tcctgcgcat taaaatgcaa ggcagtacaa atgctaatat
      961 cgaccgttgc cagttactgg agcaactcct aattcagaaa cttctaataa cccaggccaa
     1021 aattgatagt attgaggtaa acgaaggttc agttgaaagc gaagttgacc gtcgtttaaa
     1081 gtactttatc aatcaaattg gatcggaaaa ggcactggag aactacttta acaagccgat
     1141 atttgaaatt aaggacgatt taagagaaat catacgcgag caacagctaa cccagcagat
     1201 gcagcgtaag attgtagata aggttaaaat aaccccatcg gacgtaaaag ctttttacaa
     1261 gaaaattccc tccgacagcc tacccgacat acccatgcag tacgaacttc aacagattgc
     1321 aatttacccc cccgatgccg aaaaggctaa gcttgaggct aaagaaaaac tgcttgaaat
     1381 tcgtaatcga atacttaatg gtgaacgatt ttcaaccctt gccgccctct actctgagga
     1441 tagagcatcg gccataaagg gcggagagtt gggtttccgt tcacgcgaag aacttgttaa
     1501 ggcatttgcc gatgccgcat ttaaccttaa ggaaggtcaa attagccagg tggtggaaac
     1561 cgagtacgga ttgcacatca tccagatgat tgaacgacga aacgaccagg taaatgttag
     1621 gcatatcctt ctcaaacctc agttcacaac cgatttgctt tcaaaagcag ttcaaaagct
     1681 cgacagcatt gccacgctta tccgcaacga tagtattacc tttgagaacg cagcactccg
     1741 tttctccgag gataaaaaat caaacctaaa caagggtttg atgatcaatc cctttaccaa
     1801 tacctcgcta tttgagaaag agcacttgca gccatcggac ttttatgcaa taaaagacct
     1861 aaaagagggc gagtatagcg caccctttga gtcgcgcgat gagcatgcaa acatcgtttt
     1921 taaaatcatc aaggtgaaac gaattgtgcc agcccataag gcaaacctga aagacgatta
     1981 cgataaaatt caggaaatgg ccaagttgag taaagaaaac gagatactca gggactgggt
     2041 atcaaaaaaa ataaaatcgt tttatgtcag aattgataac gattttcatg gttgcaattt
     2101 ccaaagcaag ggttggataa agtaggcata tttgagatga aatccgtact tcgtattttt
     2161 ttcattgtaa ctattcttct gggattgcac acaggcgctc atgctcaaca aaaaagccga
     2221 attaggtttg aaggggcaaa gagcatgaaa aatctcaata ttaatggcca gaaaattatc
     2281 aggtatatag ggaatgttcg ctttcggcat gagaataccc gtatagtttg cgacagcgct
     2341 taccaaaatc ccgacgataa ctggtttgat gcctttggta atgttagcat tcaacaaggt
     2401 tcaacccgaa taaccgggca aacccttcac tttgatggca aaacctcagt tggtaagatc
     2461 acaggtaaag aggtaaaact tatcgacgaa gaaaccacgc ttactaccca aaccctcttc
     2521 tttaactcaa aaacaaatgt tgcctactac accactccgg gtgtaattac ctcaaaggag
     2581 tcgaaactct caagccagcg cggctacttt aaccgcttct caaacctcct atcgtttgct
     2641 ggaaacgtag tgatggtaga ccccaatgga acaatataca ccgattccct tagctacaat
     2701 acccaaaatg aacttgctta cttttttggt ccaacccaca tctttaatga ggataattat
     2761 gcctattgcg aaaagggttg gtacaataaa aaaattgagc aaaccaatct acagcaaaat
     2821 gcttacatac ttagccagga gaataaaata tttggcaaca acatttttta cgacaaatct
     2881 attaagtttg cccatgccat tggaaatgta gtgctagttg ataccttaaa cagggcttac
     2941 atctatggcc aaaaagccaa tttttggcag gaaactgagc aagccgaggt tacggagaac
     3001 ccctacctga tgctaattga taattccgat agtttattcc ttaaagcaaa ctactttaaa
     3061 gtaattaccc aaaagcatga aaactcaacg gactcatcct atagagttct taaagcatta
     3121 ggcaatgttg cattttacag gcacgatgta caaggatttt gcgactcaat gatatacaat
     3181 acccgcgact cagcaattga tatgtatgtt gatcctattc tatggaatga tgtaaaccaa
     3241 atgaccgccg accacattac cattcattca aaaaataaca aaataaacca aatggatttt
     3301 aacggcaatg cctttattac ctcatttgag caagacaaat tttacaacca aattaaaggt
     3361 aaaaccataa ccgcattttt tagtaatggc aaagccgaaa agattgatgt aaatggaaat
     3421 ggacaagcgg tttactttat ccgcgatggc aataccctta ccttagccaa ccgctccgag
     3481 agcgcaagaa tttcaattat ggtaaaggat aataaagcaa gccgggtaac tttccacgag
     3541 aaaccatcat caaaccttta ccccattgag aaggttgaac tggaagatgt tatgcttaag
     3601 ggtttcaaat ggctcgaggc taaacgaccg gctaa
//
LOCUS       DAOOWJ010000177         3522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_26699_length_3522_cov_6.433516, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000177 DAOOWJ010000000
VERSION     DAOOWJ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26699_length_3522_cov_6.433516"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /locus_tag="PLW22_11585"
     CDS             <1..1898
                     /locus_tag="PLW22_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOU75386.1"
                     /translation="EILSTNRAGGYFSTTIILCNTRKYHGLMILPIDEFDGENHVLLS
                     CVDETVIQHGQTFNLGIHRYPGIYEPRGHKYIIDFTYEPTPTLTYRVGGVIFKKELLF
                     VHNEEQFMIRYTLVDAHSSTHLRIKPFLAFRNVHNVSKANMWANTKFQPCANGVKYKL
                     YTGFPTLHLQLNKKNEFVACPDWYYNIEYMEELKRGYEGHEDLFVPGYFEIPIKKGES
                     VIFSAATEEQTPSTLLKKFNAEILRRPAKDSYKACMENSARQFIVHKGKSWDIIAGYP
                     WFGSWGRDSFISLPGLTLGIDDPKMCKTLLDSLSAKLKNGLFPNMGNATSQAFNSVDA
                     PLWYFWAIQQYYLYTNDHKEVWHDYAKKMKTILKGFRDGLPFNIKMHDNGLIWAGERG
                     YALTWMDAVVYGKPVTPRIGYPVEINALWYNAIMFTIDLATKNKDYRFVKEWLPIAER
                     AKASFNNLFWLAEKGYLADFVDEDGPNTDIRPNQIFAISLPYTPLDEDKMPSVLKIVE
                     KELYTPKGLRTLSPNNPHYKGRYEGDQPTRDSAYHQGTAWPWLLGAYVEAHFRLNPEH
                     AINVAENILESFQEDISIHGICTISEVYDGDPPQRPNGCISQAWSVAELIRIYQMVEK
                     HKSGKGK"
     gene            1912..3183
                     /locus_tag="PLW22_11590"
     CDS             1912..3183
                     /locus_tag="PLW22_11590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU75387.1"
                     /translation="MFGWEFPPHITGGLGTACYGLTRALAKQGTEIIFVVPKAYGDED
                     QRRVRLVSAEDIEVSHSSKNLEEFWKHIKYLEISSNIVPYVDPEEFSKIVEYAENESV
                     EYNINTFKRKFKFTGKYGPNLMTEVTRYAITAASVAWRETFDVIHAHDWLTYPAGIAA
                     KMVSGKPLVVHVHATEFDRSGENVNQAVYDIERRGMEVADMVVTVSNLTRNIVINRYG
                     IPEHKVVTVHNAVHFKGDIEQEFERGVDEKVVTFLGRVTFQKGPDYFVEAANKVLKRT
                     QNVRFVMAGSGDMLNRMIRRVAQLGIAPRFHFTGFLKGNEVNQMFQLSDVYVMPSVSE
                     PFGISPLEAMRSSVPVIISKQSGVAEVLKYAIKVDFWDIDALADSIYGLLNYQALSNF
                     FIKYGQEEVNSLKWDGAATKIIEIYNKLVNR"
     gene            3195..>3522
                     /locus_tag="PLW22_11595"
     CDS             3195..>3522
                     /locus_tag="PLW22_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOU75388.1"
                     /translation="MKTICFYFQVHQPFRLRRYRFFDIGKNHNYFDEFANRSIMRKVA
                     EKCYLPTNKLMLDLIKEYGSRFKISYSISGTALDQFEMYAPDVLETFQKLAKTGSVEF
                     LAETYSH"
BASE COUNT         1018 a          778 c          811 g          915 t
ORIGIN      
        1 gggaaattct aagcacaaac cgtgcaggtg gatatttcag cacaacaatt attctttgca
       61 atacacgcaa gtaccatggc ttgatgattt taccaattga tgagtttgat ggtgaaaatc
      121 acgttttgct ttcgtgcgtt gacgaaaccg taatacagca cggacaaacg tttaacctgg
      181 gtattcatcg ttaccccggc atttacgagc ctcgtggcca taaatacata atcgatttta
      241 cctacgagcc aacacctacc ctaacctacc gggttggtgg cgtaatcttc aagaaagagc
      301 tgctgtttgt gcacaacgag gagcagttca tgattcgcta caccttggtc gatgcgcata
      361 gctcaacaca cctgcgaatt aaaccttttc ttgccttccg caatgtacat aatgtttcaa
      421 aggccaatat gtgggctaac acaaaatttc aaccatgtgc caatggggtt aagtataagc
      481 tttacacagg gttccctacg ctacacctac agctgaacaa gaaaaacgaa tttgtagctt
      541 gccctgattg gtactacaac attgagtaca tggaggaact gaaacgtggg tacgaggggc
      601 acgaggacct ttttgttcca ggttacttcg agattccaat aaaaaagggc gaaagcgtaa
      661 tcttttcagc cgccaccgag gagcaaacgc cttcgacctt acttaaaaag tttaatgcgg
      721 aaatactccg acgcccagcc aaggatagct acaaagcttg catggaaaac tctgcccgac
      781 agtttattgt tcataaaggg aaaagctggg atataattgc tggttaccca tggtttggaa
      841 gttggggacg cgattcgttt atatcgctac cgggactaac attaggcatt gacgatccta
      901 aaatgtgcaa aacactgctg gactcgctta gcgcaaagct caagaatgga cttttcccca
      961 acatgggaaa tgccacatcg caggccttta actcggttga tgcacccctt tggtatttct
     1021 gggctattca gcaatactac ctatatacaa acgaccacaa ggaggtatgg cacgattatg
     1081 ccaagaagat gaaaacgatc ctcaaaggat tcagggatgg attaccattt aacattaaga
     1141 tgcacgacaa cgggcttatt tgggcaggag agcggggtta tgcccttaca tggatggatg
     1201 ccgtggttta tggcaaaccc gtcacacccc gaattggcta tccggttgaa ataaatgcct
     1261 tatggtacaa cgctataatg tttaccattg accttgccac caagaacaag gattaccgtt
     1321 ttgtaaagga atggctacct attgccgaaa gggcaaaagc atcgttcaac aatcttttct
     1381 ggcttgccga gaagggctac ttagccgatt ttgttgatga ggatggcccc aataccgata
     1441 ttaggccgaa tcaaatcttt gccatttcgc tgccatacac accactcgat gaagataaaa
     1501 tgccaagtgt gcttaagatt gtcgaaaagg agctatatac acccaaggga ctacgaacac
     1561 tctcgccaaa caatccgcat tataagggga gatacgaggg cgaccaaccc acacgcgact
     1621 cagcctacca tcagggtacg gcatggccat ggctcctagg agcctatgtt gaggctcatt
     1681 tccgccttaa ccccgaacac gccattaatg tggctgaaaa tattcttgaa tcgttccagg
     1741 aagatattag tattcacggt atttgcacca tttccgaggt ttacgatggc gatccccctc
     1801 aacgtccaaa cggctgcatt tcgcaggcat ggagcgttgc tgaacttata agaatatatc
     1861 aaatggttga aaaacacaaa tcaggaaagg gtaaataata tgagagttct gatgttcggt
     1921 tgggagtttc cccctcacat tactggaggt ttaggaaccg cttgctatgg acttacccgg
     1981 gcattggcta aacagggcac tgaaattatt tttgttgtgc ccaaagctta tggcgatgaa
     2041 gaccagcgta gggttcgcct tgttagcgcc gaagatattg aggtgtcgca ctcatccaaa
     2101 aaccttgaag agttctggaa acatattaaa tacctggaga tcagctcaaa catagttccc
     2161 tatgttgatc ctgaggaatt tagtaaaatt gttgagtacg ctgagaacga gagcgttgag
     2221 tacaatataa acacatttaa aagaaaattt aagttcacag gaaagtacgg ccctaaccta
     2281 atgaccgagg ttacccgcta cgctattact gctgcatcgg tggcttggcg ggaaacgttt
     2341 gatgtaattc atgctcatga ctggcttacc taccccgctg gcattgcagc caaaatggta
     2401 tcgggaaaac ccttggttgt tcacgttcac gcaaccgaat tcgaccgcag tggcgagaat
     2461 gtaaatcaag cggtttacga tattgagcgt cgaggcatgg aagttgccga catggttgtt
     2521 accgttagca acctaacccg taacatcgtg ataaaccgtt atggtattcc cgaacataag
     2581 gtagtaactg ttcacaacgc cgttcatttt aagggtgata ttgagcaaga atttgagcga
     2641 ggggttgatg agaaagtggt tacattccta ggaagggtta cttttcagaa aggacccgac
     2701 tactttgtgg aggcggccaa caaggtgctc aaacgcaccc aaaatgttag gtttgttatg
     2761 gctggaagcg gcgatatgct aaaccgaatg attaggcgtg ttgcgcaact tggcattgcc
     2821 ccaaggtttc actttacagg cttcctcaag ggaaacgagg taaaccagat gttccagcta
     2881 tcggacgttt atgttatgcc atcggtatcg gaaccatttg ggataagccc gcttgaggcc
     2941 atgcgctcaa gcgttccggt tattatatca aaacaatcgg gtgttgccga ggtgcttaag
     3001 tatgccatta aggtcgattt ttgggatatc gatgcccttg ccgatagtat ttacgggctc
     3061 ctcaactacc aagccctttc aaacttcttt atcaaatacg gacaggagga ggttaacagc
     3121 cttaagtggg atggtgcagc aaccaagatc attgaaatat acaacaaact agttaacagg
     3181 taaattggaa tactatgaaa accatttgct tttacttcca ggttcatcaa cctttcaggt
     3241 tgcgtaggta tcgctttttc gatatcggga aaaaccataa ttactttgac gagtttgcca
     3301 ataggtcgat aatgcgaaag gttgccgaga aatgctacct acctaccaac aagctgatgc
     3361 tggacctcat aaaagaatac ggttcaaggt ttaaaatatc ctactccatt tctggtactg
     3421 cgctcgacca gtttgagatg tatgctcccg atgtgcttga aacattccag aagctagcca
     3481 aaaccggtag tgttgagttt ttggctgaaa catactcgca ct
//
LOCUS       DAOOWJ010000178         3427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_27684_length_3427_cov_3.510083, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000178 DAOOWJ010000000
VERSION     DAOOWJ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27684_length_3427_cov_3.510083"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..746)
                     /locus_tag="PLW22_11600"
     CDS             complement(147..746)
                     /locus_tag="PLW22_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75389.1"
                     /translation="MKHKLSPIIIAVLTTVMLVNTLSAQQKRTNWLQEKNEYTGRWRV
                     GVGANIVEPTAVDVQFYRLSKICTSQFAINKKIAIGIWAGMEGLLLGSLIERQNNKTW
                     EKGSIRYGIDAKFYLPIALNPYFGFGIEGGNRTLNNESTFSPDFVARIGLEQKIAGIK
                     LSTRSALNITFFIDGKLNKCLNVDFVYIMPSLGLRFHWI"
     gene            1151..2656
                     /locus_tag="PLW22_11605"
     CDS             1151..2656
                     /locus_tag="PLW22_11605"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOU75390.1"
                     /translation="MNDSEILMNPNELVRFLKKPASEFTKNDIIRFCEANNIQMVNFR
                     YVAEDGKLKTLNFVISSKDHLDSVLSAGERVDGSSLFSFIEAGSSDLYVIPRYRTAFV
                     NPFSDVPTIDILCSFYDSTGKPLESAPENVLRKAHDLFKSETGMTFKALAELEYYVIS
                     EREDFYPGVDQKGYHASEPFAKWENLRREALIYIASAGGKVKYGHSEVGCFMTDEYLY
                     EQHEIEFLPMPAEEAVEQLIIAKWIVRMLGYRYGVDISFAPKITVGKAGSGLHFHMMV
                     EKDGKNMMVENGKLSNTAKKMVAGILDAADALTAFGNTIPISYLRLVPHQEAPTNICW
                     GERNRSVVIRVPLGWIGAEHMVQHANPNDCSVYEAKESKQTVEYRVADGSANPYLTVA
                     GLIVAALDGLRRPDALELAEKLYVDVNIFRPEFKERLASLKQLPASCWESAEALEAKR
                     QKFEKDGIYPHGMIDSIISKLKAFNDKGLSEKLYGNNAEIAKLVSQFLHIA"
     gene            complement(2703..3365)
                     /locus_tag="PLW22_11610"
     CDS             complement(2703..3365)
                     /locus_tag="PLW22_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75391.1"
                     /translation="MRKNSSSGFNILWAALILFALQPSRLNAQWYAEASGGGVYNLPM
                     PLAIYQDGTPTIRMTARFKTEPFTLPVYWDVRFGKIVGKRIYEAELIHHKLYLKNTTA
                     EVQKFNVSHGFNMLIFNYGIERGKYRYRAGLGLVIAHPESKIRGLVFGNSTDDWDMGY
                     YLTGPVVSASAERKIDMSKHVYLLFGAKTTVALAKVPINGGNARFVNVAGHLNLGVGL
                     RK"
BASE COUNT          979 a          761 c          720 g          967 t
ORIGIN      
        1 aggggtattg tcattccgag ctagtcgagg aatctcatta aagggatgaa aggacaaaga
       61 gatgcttcgg cagcgctcag catgacaaat atccctatct taacgatatt aaataaattt
      121 tactttccaa acatttttct aatgcactaa atccagtgaa aacgcaatcc aaggcttggc
      181 atgatgtaga caaaatcgac attcaggcat ttatttagct tcccatctat aaagaatgtg
      241 atgttcagtg ccgaccgtgt ggatagttta attccggcaa tcttttgttc aagcccaatg
      301 cgcgcaacaa aatcgggcga aaaagtgctc tcgttattta aagttctatt tccaccttca
      361 ataccaaaac caaagtacgg gttaagggca atgggcaggt aaaacttggc atctattccg
      421 tagcgtatgc tccctttctc ccaggtcttg ttattctgcc tttctatcag ggagcctaat
      481 agcaaacctt ccatgccagc ccaaatacca atggcaattt tcttgttaat ggcaaattgg
      541 cttgtgcaga tcttgctcaa acggtaaaac tgaacatcca ccgctgttgg ctcaacaata
      601 ttggcaccaa caccaacacg ccagcggcct gtgtactcgt ttttctcctg caaccagttt
      661 gttctcttct gctgggcgga caacgtattt accagcatta ccgtagttag aacagctatg
      721 attatgggtg aaagtttatg tttcatgcag tgacagattt tttccacctt tactagttat
      781 acttaaaagt taacagtgca atttaacgaa attattgtcc attgctgtga ttttgcctgc
      841 aattaaaaat tagatggtat ttgcctaagc tagctgcaat catttaatga catctgcgaa
      901 ccgttggaaa gatactcaaa catatttaat cgcctaacca attcaaaaat tgactcgaac
      961 tcacattcgg cagaaatata tcgagataaa gcctttggtt ttagaaaaaa tgtctatgca
     1021 ttttgtctat tattgaaaat acttctactt taagggaaaa tgctataaaa tgatgttgat
     1081 aaacatagcc gcaaacgaat gcgatgtcga ttgattttgt agctttggca aaataaaaaa
     1141 ttaggaacac atgaacgatt ctgaaattct gatgaaccct aatgagctgg ttcgctttct
     1201 gaagaaaccc gcatcggagt ttaccaagaa cgatattatt cgcttttgcg aggctaacaa
     1261 cattcaaatg gttaacttcc gctatgtggc cgaggatggc aagctaaaaa ccctgaactt
     1321 tgtaatatca tcaaaggatc acctcgattc cgttctatcg gccggagaga gggttgatgg
     1381 tagtagtttg ttctcgttca ttgaggccgg aagtagcgac ctttacgtta taccacgtta
     1441 ccgtaccgct tttgtgaatc ccttttccga tgttcctaca attgacattc tttgctcatt
     1501 ttacgatagc accggcaaac cccttgaaag cgctcctgaa aatgtgctac gcaaagctca
     1561 cgatttattc aagagcgaaa cgggcatgac ctttaaggca cttgcagaac tggagtacta
     1621 cgtgattagc gaacgcgaag atttttaccc gggtgtcgat caaaagggtt accacgcctc
     1681 ggaaccattt gcaaagtggg aaaacctacg tcgcgaggct cttatttaca ttgccagtgc
     1741 tggaggcaag gttaagtatg gccactcaga ggttggttgc ttcatgaccg acgaatatct
     1801 atacgagcag cacgagattg aattccttcc catgccagct gaggaagcag ttgaacagct
     1861 tataattgcc aagtggattg tacgcatgct tggctatcgt tatggagtag atattagctt
     1921 tgccccaaaa atcactgtag gaaaggcagg aagcggctta catttccaca tgatggtaga
     1981 aaaagatggc aaaaacatga tggttgaaaa cggtaagctt agcaataccg ccaagaaaat
     2041 ggttgccgga atcctcgatg cagccgatgc ccttaccgcc tttggtaata ccatacccat
     2101 atcgtacctg cgccttgttc cacaccagga agcacctact aacatctgct ggggtgagcg
     2161 taaccgttca gttgtaatac gtgttccgct tggatggatt ggtgctgagc acatggttca
     2221 acatgccaat cccaacgatt gcagcgtata cgaggctaaa gaaagtaagc aaaccgttga
     2281 gtatcgtgtt gccgatggat cagctaaccc ttacctaact gttgccggcc ttattgttgc
     2341 tgcactcgat gggcttcgtc gccccgatgc acttgagcta gccgaaaagc tatatgttga
     2401 tgttaatatc ttcaggccag agttcaagga gaggcttgcc agcctcaagc aattacctgc
     2461 atcgtgctgg gaatcggccg aggcgcttga ggccaaaagg caaaagtttg aaaaggatgg
     2521 tatttaccca cacggcatga tcgactcaat tatttcaaag ctgaaagctt ttaacgataa
     2581 ggggctaagc gagaaactat acggaaataa cgccgaaata gccaagctgg tatcgcagtt
     2641 cctacatatt gcttaacatt tcagggaata tatcaatgcc gatccaatag gatcggcatt
     2701 ttttactttc tcaacccaac tccaaggttc agatgccctg caacgttcac aaaccttgca
     2761 ttaccaccat tgataggtac tttggcgagt gcaactgtag tttttgctcc aaataaaagg
     2821 taaacgtgct ttgacatgtc gattttacgc tcagcgctgg ctgaaactac aggcccagta
     2881 aggtagtagc ccatatccca atcatcggtt gagtttccga acacaagtcc acgaattttc
     2941 gactccgggt gtgcaattac aagccctagc cctgctctgt accggtattt acccctttca
     3001 attccgtagt taaaaatcag catgttgaac ccatgcgaaa cgttaaactt ttgcacttcg
     3061 gctgtagtgt ttttcaggta cagcttgtgg tgtattaact cagcttcata gattcttttc
     3121 ccaactattt ttccaaatcg aacatcccag taaaccggta aagtgaaagg ctcagtttta
     3181 aatcgggctg tcattctaat tgttggtgtg ccatcctgat aaatagctag cggcattggc
     3241 agattataga cacccccacc agaggcctca gcgtaccatt gagcatttaa tctgcttggc
     3301 tgtagggcaa aaagtataag tgctgcccaa agtatattaa agccactgct tgagtttttc
     3361 cgcaatattt tgcgatgctt tcccatttcc atacaaatcg atgtcgaagt tcgatctttt
     3421 atttatc
//
LOCUS       DAOOWJ010000179         3383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_28136_length_3383_cov_5.704327, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000179 DAOOWJ010000000
VERSION     DAOOWJ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28136_length_3383_cov_5.704327"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..2531)
                     /locus_tag="PLW22_11615"
     CDS             complement(81..2531)
                     /locus_tag="PLW22_11615"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOU75392.1"
                     /translation="MERLLSVIFIVFATTNLAFSQGQITKVRGKVFDAQTNEPLPFVN
                     VTFKNTTQGTITSEKGEFFLESRAATDTLLVSFVGYKSVAIKVKKGVYQEISVNLEPE
                     AIELDAVVVKPGESQANRIIRNIIKYKDRNNPANFTYSCKSYNKIQVDINNIEEDIKK
                     RKVLNQFQFIWDYVDTNAVTGKTYLPIFITESLSDYYYSANPKMEKEIIRATKMSGVN
                     NESVAQFTGKIYQSVNIYDNYINIFDQGLVSPISNSGLLFYKYILLDSMHIDNRWCYQ
                     ISFRPRRKMEPTFTGDFWVNDSTWAIVKAQIRLSDMVNMNFVNDMVATAEFMPLNDTL
                     WFPKQMTLFVDFNLTDKTTGFFGHKTISYSDVKLNAEFPKEVTDLPTNLKVEEGALNQ
                     EEEFWAQARPFELTPREANIYQMVDSVQQVPMYKTFIDIINMFVNYYYVVGYWEIGPY
                     YQTYSFNEIEGNRFKVSGRTSNKFSKRVMFDAFVAYGDKDERFKWGVGALYMISKNPR
                     EAVGVSYKSDIEQLGQSPYALTEDNILTSFLRRNPNYKLTLVKDFTAYYEKEWFVGLS
                     NKVTFSHRIIFPTEFIPFTPVSGGSSLKSITNATLTLNTRWIKDERFVSGEFEKVSLG
                     SNWPEVNFNITRSIKGFAGSDYDFWKLHLNYYHKFNLNPIGYARFIVDAGKIFGKVPY
                     PLLQLHEGNETYAFDRYAFNMMNYYEFASNQYVSLYYEHHFQGFFLNHFPLLRRLKWR
                     EVATAKYLIGSISNENMQMLQFPAGLGEVSKPYMEVSVGLENIFKVVRVDALWRLTHL
                     DNPNIEPFGVRVGLQIIF"
     gene            complement(2538..3323)
                     /gene="tatC"
                     /locus_tag="PLW22_11620"
     CDS             complement(2538..3323)
                     /gene="tatC"
                     /locus_tag="PLW22_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOU75393.1"
                     /translation="MKSRSEMTIVEHLAELRMVLLKVIAAIALGSVIAFLLGNNILDL
                     IFQVSNPQFITNRWFCRLATLLNSPDLCINSTPLGLVNIELAGQFSLHIKLAVLGGVI
                     LTLPYCITVIAGFILPALKEKESKLSLRFLLLSFALFIVGVAFGYYVIAPIAVHFMAN
                     YNLSKIIVNHITVQSFIATVSQTVLAMGIGFELPLVVFFLTKWHIIELSLLEHNRPVA
                     IVVLLILAAIITPPDVFSMMIVVLPLWLLYEISIFATKWAKLK"
BASE COUNT         1015 a          733 c          674 g          961 t
ORIGIN      
        1 cctgccttaa atcgtacttc acacacgagg tgttttccct gcttattaac cgattgaaat
       61 caaaatccat gcttaaagat ttagaatata atttgcaatc caacccgaac cccaaaaggc
      121 tcgatgtttg gattatccaa gtgggttagg cgccaaaggg cgtcaaccct cactactttg
      181 aaaatatttt ccaaaccaac acttacctcc atgtagggct ttgaaacctc acccaagccg
      241 gcagggaact gcagcatctg catattttcg ttacttatgc tcccaattag gtatttggcg
      301 gtggcaacct cgcgccattt taggcggcgc aaaagcggga aatggttaag gaaaaaaccc
      361 tgaaaatggt gctcgtagta taggctaaca tactgattcg atgcaaactc atagtagttc
      421 atcatgttaa aggcatacct gtcaaatgca taggtctcgt taccctcgtg tagctgaagc
      481 aagggatacg gaactttccc gaaaattttg ccagcatcca caataaatcg ggcgtagcca
      541 attgggttga gattgaattt atggtagtaa tttaggtgta gcttccaaaa atcgtaatcg
      601 gaaccggcaa atccttttat tgacctggta atattaaagt taacctcggg ccaattactg
      661 ccaagactca ctttctcgaa ctcgcccgaa acaaagcgtt catctttaat ccaacgggta
      721 ttaagggtga gtgtggcatt ggtaatgctc ttaagactgc ttccgcccga aacaggtgta
      781 aatgggataa attcggttgg gaaaataatc ctgtgactaa aggtaacctt attggacagg
      841 ccaacaaacc attccttttc atagtatgca gtaaaatcct ttacaagcgt caacttatag
      901 ttagggtttc gcctaaggaa cgatgtaaga atgttatcct ccgtaagcgc ataggggctt
      961 tgcccaagct gttcaatatc gctcttatag ctaaccccaa ccgcttcgcg tggattttta
     1021 ctgatcatgt aaagtgctcc caccccccac ttgaaacgct catccttatc gccgtaggct
     1081 acaaaggcat cgaacataac ccttttgctg aacttattgc ttgtgcggcc tgaaacctta
     1141 aaccgattac cctcaatctc gttgaagcta taggtttggt agtatggccc tatttcccag
     1201 tatcctacta catagtagta gtttacaaac atattgataa tatcgataaa tgtcttatac
     1261 atgggaacct gctgaacgga atcgaccatt tggtaaatgt tggcttcgcg cggggtaagc
     1321 tcaaacggac gtgcctgtgc ccaaaactcc tcctcttggt ttagcgcgcc ctcctctact
     1381 ttaaggttgg taggcaagtc tgtcacttcc ttgggaaact cagcgtttag cttaacatcg
     1441 ctgtacgata ttgttttatg gccaaagaat ccggttgttt tatcggttag gttgaaatca
     1501 acaaaaaggg tcatctgctt agggaaccaa agggtgtcgt tcagtggcat gaactcagca
     1561 gtagccacca tatcgttcac aaagttcatg tttaccatat ccgataatct aatttgagcc
     1621 tttacaatag cccacgtgct atcgtttacc caaaaatcac cggtaaaggt aggttccatt
     1681 ttacggcggg gacgaaacga aatctggtag caccaccggt tatctatatg catgctgtcg
     1741 agcagaatgt acttgtaaaa aagcagccct gagttggaaa ttgggcttac cagcccctgg
     1801 tcaaaaatgt tgatgtagtt gtcgtatata ttaacgcttt ggtaaatctt accggtaaac
     1861 tgcgccacgc tttcgttatt cacccccgac atcttggtag ccctaataat ctccttttcc
     1921 attttgggat tggcgctgta atagtaatcc gatagcgatt cggtaatgaa tattggcagg
     1981 taagttttac cggttacagc attggtatca acataatccc atataaactg aaactggttt
     2041 aatactttac gtttttttat atcctcctcg atattattga tgtcaacctg aattttattg
     2101 tagcttttac agctataggt aaagtttgcg ggattattcc tatccttata cttaatgatg
     2161 ttacggataa ttcgatttgc ttgactctca ccaggtttta caaccacagc atcaagctct
     2221 atggcttcgg gttccaaatt tacggatatc tcctggtaaa cacccttttt aaccttaatc
     2281 gcaacggact tataacccac aaacgaaacc aaaagggtat cggttgcggc acgcgattca
     2341 agaaaaaatt cgcctttttc ggatgtaatg gtaccctgag tggtattctt aaaagtaaca
     2401 ttaacgaagg gtaaaggctc attggtttgg gcgtcaaaaa ccttgcccct caccttggta
     2461 atttgtcctt ggctgaatgc caggtttgtt gtagcaaata caataaatat gactgataaa
     2521 aggcgttcca tagcttatca cttaagtttt gcccatttag tggcaaaaat gctaatctca
     2581 taaagcaacc aaagcggcaa tactacaatc atcatgctaa acacatcggg cggtgtaatt
     2641 atggcagcta aaataagtaa aacaacgatg gctactggac gattgtgctc aagcaaactg
     2701 agctcaatga tatgccattt agtcagaaag aaaactacta aaggcagttc aaatcctatt
     2761 cccatcgcta atactgtttg cgacactgta gctataaacg attgaacagt aatatggttc
     2821 acaataattt tgctaagatt gtaattagcc ataaaatgaa cagcaatggg tgctataaca
     2881 tagtaaccaa atgcaacacc cactataaat agagcgaaac ttaaaagtaa aaatcttagc
     2941 gaaagtttac tttccttttc tttcagggca ggtaaaataa agccggcaat gacagttatg
     3001 caatatggta atgtaagtat aacacctccc agaactgcca atttgatatg gagcgagaat
     3061 tgccctgcaa gttcaatgtt aactaatcca agcggcgtgc tattaataca taagtcgggt
     3121 gagtttaaaa gagttgccag cctgcaaaac caacgattag tgatgaactg tgggtttgaa
     3181 acctgaaaaa tcaaatcgag aatattattg cccaacagaa aggcaatcac actccctaat
     3241 gcaatggcag caataacctt cagtaaaacc attcgtagtt cagccaggtg ctcaactatg
     3301 gtcatctccg acctgctttt catggcaaaa aggataggtt aaactatacc ctcgcgaagt
     3361 atatcgtgaa tgtgaattac acc
//
LOCUS       DAOOWJ010000180         3367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_28314_length_3367_cov_7.084843, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000180 DAOOWJ010000000
VERSION     DAOOWJ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28314_length_3367_cov_7.084843"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLW22_11625"
     CDS             <1..393
                     /locus_tag="PLW22_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOU75394.1"
                     /translation="YQQLAIIRAMVRMLGYRIEIVGCPIVREPDGLAMSSRNLLLTPE
                     HRKSAPLIYQTLAEARNKTNEFSVKDMIDWVVNRINSDPNLKVEYFELADADSLLPVQ
                     GWDHPNGIVGCIAVWAGNIRLIDNMMFK"
     gene            415..765
                     /locus_tag="PLW22_11630"
     CDS             415..765
                     /locus_tag="PLW22_11630"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOU75395.1"
                     /translation="MMIEVCKSKIHRVTITEANLNYIGSITIDEDLMDAANIIENEKV
                     QIVNINNGERLETYVIKGERGSGQVCLNGPAARKCVVGDVVIIISYATMDFEEAKKFK
                     PTVIFPDTATNKLV"
     gene            783..1787
                     /locus_tag="PLW22_11635"
     CDS             783..1787
                     /locus_tag="PLW22_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU75396.1"
                     /translation="MKSIVKSRLFNFAVFFSLAIVLLYYAFRSVDFTHIAQGFREVNY
                     FWIAISLLIALIAHALRAIRWGYLMESLGHKPPFSNLFAAVMFGYLANLALPRFGEVA
                     KCGSVSRVSRIRFESLVGTVVVERAADLAMLLIATVIVVLVKIDSFGQYIYTKIVHPI
                     EQKAIHIEGYKIVILLVMAIAFLLLIRFILKSDFLGQKLNGKIKSAVIGVIDGLKSIY
                     RTPRVIQFALLTVLIWVSYWLMTWALLCSTPITSNLTGWDALFIMVIGSYGMTVPVQG
                     GFGAYHIITASALGIFGITYENGLIFAIISHESQTIMVVIAGIVSMVYLYIKQRKPLA
                     "
     gene            1968..3236
                     /gene="metK"
                     /locus_tag="PLW22_11640"
     CDS             1968..3236
                     /gene="metK"
                     /locus_tag="PLW22_11640"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOU75397.1"
                     /translation="MGYLFTSESVSEGHPDKLADQISDALLDEFLRRDPNSKVACETL
                     VTTGLVVLSGEVKTHAYVDVQEIARRVIRRIGYTKHELRFDAESCGVISSIHEQSPDI
                     NRGVERGDEFSQGAGDQGMMFGYACRETDDYMPLALVLSHVLLMELAKIRREGKQMAY
                     LRPDAKSQVTIEYDDNNQPLRIHTIVVSTQHDEFDTDDVRMLNKIKEDVRTILIPRAK
                     ARFPERVQKLFKDDYILHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFS
                     GKDSSKVDRSGAYAARHIAKNMVAAGIADEVLIQVAYAIGVAEPVGLYVNTYGTSHVK
                     LTDGQIAEKIKQIFDLRPGAIVKRLGLKNPIFEETAAYGHFGRDYYKREVEFIVNGNC
                     EGEKVVKREVEFFTWEKLDYVDILRKEFNL"
BASE COUNT          863 a          745 c          811 g          948 t
ORIGIN      
        1 taccagcagc tggccattat tagggcaatg gttcgtatgc tgggctaccg gattgaaata
       61 gttggctgcc ctattgtgcg tgaacccgat gggttggcca tgagcagccg taacctgctg
      121 cttacccctg agcatagaaa aagcgcccca ctcatttacc aaacattagc tgaagcacga
      181 aacaaaacca atgagttttc cgttaaagat atgattgatt gggttgtaaa tagaatcaac
      241 tctgacccaa atctaaaggt tgagtacttt gagctggccg atgccgattc cctgctgcct
      301 gttcagggct gggatcaccc taacggaatt gttggttgca ttgcggtttg ggctggtaat
      361 atcaggctta tcgataacat gatgtttaaa tagttccgaa aacagtataa ggcgatgatg
      421 attgaagttt gcaagtcgaa aatccacagg gttactatca ccgaggctaa ccttaactac
      481 attggtagca ttaccattga cgaggacctg atggatgcag caaacattat tgaaaacgaa
      541 aaggttcaga ttgtcaatat aaacaacggc gaacgccttg aaacctacgt gataaagggt
      601 gagcgtggta gtggtcaggt ttgcctcaat ggacctgctg cacgcaagtg cgtggtgggc
      661 gatgtggtaa ttatcatttc ctatgctacc atggattttg aggaggccaa aaagtttaag
      721 cccaccgtaa ttttccccga caccgccacc aacaagcttg tataacaaac ccgtttactg
      781 cattgaaaag catagtgaaa tcaaggctat ttaactttgc cgtattcttt tctcttgcca
      841 ttgtactgct ctactacgct ttcaggagtg tcgattttac ccatattgct cagggtttta
      901 gggaggtaaa ctatttctgg atagccattt cgcttttaat tgcccttatt gcccatgctc
      961 ttagggctat tcgctggggt taccttatgg aatcccttgg ccacaaacct cctttcagca
     1021 acttgtttgc agcagtaatg ttcggttacc tggctaactt agccctcccc cgctttggcg
     1081 aggtggcaaa atgtggtagc gtaagtaggg tttcccgaat ccgttttgag tcgcttgttg
     1141 gaaccgttgt ggttgagcgc gctgccgacc ttgcaatgct cctgattgca actgttattg
     1201 tggttcttgt caaaatcgat tcctttggcc agtatatcta cacaaaaatt gttcatccca
     1261 ttgagcaaaa ggccatacat attgagggat acaagattgt tattctactg gtgatggcaa
     1321 tcgcattttt actccttatc cgtttcattt taaaaagcga tttcctgggc cagaagctga
     1381 acgggaaaat caaaagtgct gtaataggtg ttatcgatgg actaaagagt atttaccgaa
     1441 cacccagggt aatccaattc gcactgctca cggttttaat ttgggtaagc tactggctaa
     1501 tgacctgggc tttactttgc tctaccccaa ttacatcgaa ccttactggc tgggatgccc
     1561 tgtttatcat ggttattggt tcctatggca tgactgttcc agtacagggc ggttttgggg
     1621 cttaccacat tattactgcc tcggcccttg gcatttttgg aatcacttac gagaacgggt
     1681 tgatatttgc aatcatctcg cacgaatcgc aaaccataat ggtggttatt gcaggtattg
     1741 tatcgatggt ttacctctac attaagcagc gcaagccatt ggcgtagaaa attccaattt
     1801 acagcattga aagttgttac atacacgtta aaagtataca tttgtgagct gaatgtggaa
     1861 agatttggct gattgcaacc acaaaaataa atgctaaaac agcgattatt ccagcaatcg
     1921 atcaaacctt ccccatagat atttgtttaa cctaaaagtt ttttactatg ggatatttat
     1981 ttacttcgga gagcgtatcg gaagggcatc ccgataagct ggccgatcaa atttccgatg
     2041 ctctgctcga tgagttttta cgtcgcgacc ccaactcaaa ggtggcatgc gaaacactgg
     2101 ttactaccgg tttggttgtg ttaagtggcg aggttaaaac ccatgcatat gttgatgttc
     2161 aggagattgc ccgccgggtt atccgccgga ttggttacac caagcacgag ctgaggttcg
     2221 atgccgagtc gtgcggcgtt atctcatcaa tccatgaaca atcgcccgat attaaccgtg
     2281 gtgttgaacg tggcgatgag ttctcacagg gtgcgggcga tcagggtatg atgtttggct
     2341 atgcctgccg cgaaaccgac gactatatgc cacttgccct tgtgctttcg cacgtgttgc
     2401 tcatggagct tgctaaaatc cgcagggagg gtaagcagat ggcctacctc cgtcccgatg
     2461 ccaagtcgca ggtaaccatt gagtacgacg ataataacca gcctctccga atccatacca
     2521 ttgttgtatc cacccagcac gatgagtttg ataccgacga tgttcggatg ctgaacaaga
     2581 tcaaggagga tgttcgcact atccttattc caagggctaa agcccgtttc cctgaaagag
     2641 ttcaaaagct gttcaaggac gattacattt tacacgttaa cccaaccggt aagttcgtaa
     2701 taggcggtcc gcatggcgat accggcttaa ccggacgtaa aattattgtt gatacctatg
     2761 gcggtaaagg ggcccacggt ggaggcgctt tttcgggcaa ggattccagc aaggttgacc
     2821 gctccggtgc atacgcagcc cgccatattg ccaaaaacat ggttgctgcc ggcattgccg
     2881 atgaggttct cattcaggta gcatacgcca ttggcgttgc cgagcccgta gggctttacg
     2941 ttaataccta tggaacttcg cacgttaagc ttaccgatgg ccagattgct gaaaaaatta
     3001 agcagatctt cgatttgcgt ccgggtgcca ttgttaaacg tttaggcctt aagaatccca
     3061 tttttgagga aaccgctgcc tacggccatt ttggtcgcga ttactacaag cgcgaggtgg
     3121 aatttattgt gaatggcaac tgtgagggcg aaaaggtggt taaacgcgaa gtggaattct
     3181 ttacctggga aaagctcgac tatgttgata ttctccgcaa ggagtttaac ctgtaaaggg
     3241 ggttcactgt agtatttaat ttcattagag caacgctacc tgtcatcctg agcgtcctgt
     3301 cccgccatag ctgggagcaa aggatatcat tattagtgtg cagtgttcag tatgcagtgt
     3361 acagcgg
//
LOCUS       DAOOWJ010000181         3313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_28890_length_3313_cov_5.267035, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000181 DAOOWJ010000000
VERSION     DAOOWJ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28890_length_3313_cov_5.267035"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..3266
                     /locus_tag="PLW22_11645"
     CDS             342..3266
                     /locus_tag="PLW22_11645"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOU75398.1"
                     /translation="MKRLLILLTIFALILNLGFAIQKEHVKNDSLSHKSQTEAVHATN
                     MASEIENQGEVTEGSHEGHGLNLAPFLFIILSLFIGTATRHLLSKGLLPYTIMLLIIG
                     LILGLLTRFDILQAVGLGIIGQAVQWAGNIDPHLILYLFLPTLIFEAAYALHIHTFKK
                     SLVNAVILAVPGIMVAIVITAFALLLIKSMGIGLNEWTILLAFLFGAIISATDPVAVV
                     AILKEVGASKRLSTITESESMLNDGTAIVFFMTIFGLITGEAGGNAFVEFLKVSLGGV
                     LVGAVIGWGILSWLKKVFNDALIEITVVIGAAYLSFYIAESVLHVSGVIAVVTLGISM
                     AGPGKTKVSPEVTHFLHEFWELAAFIANTLIFIIVGVVIAQQVNYTLSDLKVLLLVYV
                     TIHIARLGTIYVFYPLMRRIGYGIAFKDSIVLWWGGLRGAVALALALIVAIDSRIPFE
                     PRNQLLSLTAGIVILTSLINATTVRWLIDKLGLSKIGDIKAGLMMQSLQQIRLSGEKE
                     IEKLKENRYMSGADWDKVSSFLIDANAVEEKPQHFNLEDAIAETRKRLLQKEKESYWR
                     QFSMGMLSSEGVNLLSDQIDALLDFGGKIPLSERQDIENIWTTPKTIAKLQNLPLVGR
                     IWKRKFLNRLALSYDCARAFVAAQEENQKSLSSLIIGFSLGGAESSKETELLSALEDE
                     LNENRITGQTFLRNLREKYPDICRSIETLLASRSLLNQQEEMLERLKKQGRLEPDEVE
                     RIQAYIHSSMKQLIDTSLKENTELKVAAALRNTPGLNNLSKDAQQFLITKGQLKIYPE
                     GSVIAKQDSEPDGIYIIINGTVNYYKSDTLTGVKEISDVFGAYEWFISSKLRYTFTAE
                     TPVTLIKINTSTIKLLTSKFPETNRLLQFSASADIALELLKQNEKFSEQSIRKLRNLI
                     RNAELFELNDGEKHRFSGSACILVKGQAITEEQDTLFTAPTILEECNFRSNGDALALI
                     LP"
BASE COUNT          951 a          691 c          691 g          980 t
ORIGIN      
        1 gatgccactt tccgggttaa aaacgatacc cctactacta ttgcaaagta tgcaataaca
       61 atccaaaagt aaatgttacc aagtaaagct tccataagaa attatcagtt ttttagtatt
      121 tatgttttta aaatttggct gccaaattaa tcaaatattt atcaccctat catatatttt
      181 ttaacatttt tttacccatt ccttaatttt tgtttcaaaa tgtaaccaaa actacctgaa
      241 atggaaatta ttcctaacta tctaaacaaa tggttgctga tttaaacaaa tatttgactt
      301 gcactccttt tttcgtaaat tgcttaaaat attcatttgg tatgaaaaga ttgcttattc
      361 tattgacaat atttgcccta atcctgaatt tggggtttgc cattcaaaaa gagcatgtta
      421 aaaatgatag cttatcacac aagagtcaaa ctgaggctgt tcatgcaacc aatatggctt
      481 cggaaattga gaaccaaggt gaagtaaccg aaggctcaca tgaagggcac ggcctaaacc
      541 ttgctccttt ccttttcatt attctatcgt tgtttattgg aactgctact cggcacctgt
      601 taagcaaagg gttattacca tacaccatta tgcttttaat tattggactg attttagggt
      661 tgcttacccg atttgatatt cttcaggctg ttggattggg tattattggg caagcggttc
      721 aatgggcagg aaatatcgac ccgcacctta ttctttacct cttcctgcca acccttattt
      781 tcgaggcagc ctatgccctt cacatacaca cctttaaaaa atcgttggtt aatgcggtaa
      841 tacttgccgt tcccggtata atggttgcaa ttgtgataac agcctttgct cttctgctca
      901 tcaagtcgat gggcattggc ttaaacgagt ggacaatact acttgccttc ctatttggcg
      961 ccataataag cgcaaccgac ccggtggctg ttgtggctat ccttaaggag gtaggagcaa
     1021 gcaaaaggct ttcaaccatt accgaaagcg agtcgatgct taacgatggt accgctatcg
     1081 ttttctttat gaccatattt ggcctaatta cgggcgaagc tgggggtaat gcttttgttg
     1141 agttcttgaa ggtttcgttg ggcggtgtgc ttgtgggcgc agttatcggt tggggaatac
     1201 tttcgtggct taaaaaagta tttaacgatg cgttaataga gataactgtg gtgattgggg
     1261 ctgcttacct gtcgttttac attgccgaat cggttttgca cgtatcgggt gttattgctg
     1321 tggtaacatt gggtatttcc atggcagggc caggaaaaac aaaagtgagc cctgaggtta
     1381 cccacttcct tcatgagttc tgggagttgg cagcatttat tgcaaacacc ctaattttca
     1441 tcatagttgg tgttgttatt gcgcagcagg tcaactacac tttaagcgac cttaaagttt
     1501 tgttactggt atatgttacc attcatattg cacgccttgg aaccatctac gttttttacc
     1561 ccctgatgcg ccgaattgga tatggtatag cctttaaaga ttcaattgtt ctatggtggg
     1621 gtggcctaag gggcgctgta gcgcttgcct tagcactgat tgttgccatt gatagccgaa
     1681 tcccctttga accccgcaac cagttactat cgcttactgc cggtattgtt attttgactt
     1741 cacttataaa cgcaactact gtacgttggc tcattgataa gcttggactc tccaaaattg
     1801 gcgatatcaa agcaggcctt atgatgcaat cgctacaaca aattaggcta agcggcgaga
     1861 aggaaattga aaagctcaaa gaaaacaggt acatgtccgg agccgattgg gacaaagtat
     1921 cgagtttcct tattgatgcc aatgcggtgg aggaaaaacc tcaacacttt aacctggaag
     1981 atgccattgc tgaaacccgt aagcgtctgc tccagaaaga gaaggaaagt tactggcgac
     2041 agttcagcat gggtatgctt agctccgaag gggtaaacct actctctgac caaattgatg
     2101 ccctgctcga ttttggcgga aaaattcccc tttcggaacg acaggatatc gaaaatattt
     2161 ggactacacc caaaacaata gctaagcttc agaatctccc gctagttggc agaatttgga
     2221 aacgtaaatt cctaaaccgt ttagcattga gttatgattg tgccagagcc tttgtggccg
     2281 ctcaggaaga gaaccaaaaa tctctttcga gccttattat tggcttctca ttagggggag
     2341 cggagtcgtc aaaagaaact gaactactta gcgcccttga ggatgagcta aacgagaacc
     2401 gcattactgg gcaaaccttc ctccgaaatt tacgggagaa atacccagat atttgtagga
     2461 gtattgaaac cttacttgcc tcgcgctcac ttctgaacca acaggaagaa atgttagaac
     2521 gtttaaagaa acagggacgc ctggaacccg atgaggtgga acgaattcag gcatacatac
     2581 actcctcaat gaagcaacta attgacacct ccctaaagga gaacacagaa ctaaaggtgg
     2641 ctgctgctct taggaatact cccggcttaa acaatctatc aaaagatgcc cagcagttcc
     2701 taatcactaa agggcagcta aaaatttacc ccgaaggaag tgtcattgct aaacaggact
     2761 ctgagcccga tggtatttat ataatcatca atggtacggt aaattactat aaatccgata
     2821 ccctaaccgg cgttaaggaa atttccgatg tttttggggc atacgagtgg tttatatctt
     2881 ctaagctacg ttataccttt actgctgaga cccctgttac actgattaaa ataaacactt
     2941 caactattaa attgcttacc agcaagttcc ctgaaacaaa ccgtttgctg caattcagcg
     3001 cttctgccga tattgctctt gagcttctta aacaaaacga aaaatttagt gaacaatcca
     3061 tccgtaagct cagaaacctt atccgaaatg cagagctatt tgagctgaat gatggtgaaa
     3121 aacaccgttt ttcaggttca gcatgtattc tggttaaggg tcaggctatt actgaagagc
     3181 aggatacctt gttcactgca ccgacaatac ttgaagagtg taactttaga agtaatggcg
     3241 atgctttagc ccttatttta ccttaaggct ttttagcctc aaccatgggc tgaaaacgct
     3301 tgtatgtagg caa
//
LOCUS       DAOOWJ010000182         3295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_29113_length_3295_cov_7.353395, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000182 DAOOWJ010000000
VERSION     DAOOWJ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29113_length_3295_cov_7.353395"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1146)
                     /locus_tag="PLW22_11650"
     CDS             complement(235..1146)
                     /locus_tag="PLW22_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOU75399.1"
                     /translation="MFNKLIASMLPYFPKKLVWIFSKRYIAGETIADAIRVSKELNAQ
                     GIKVTIDILGEFIKDLGEAERNKKEYLELIDTIQKENIDGNYSLKPTSFGLLIDKEIC
                     YNHIREIVAKAASYGNFIRIDMEDSPCTSMEIELYRRLKAEFPKNVGLVFQAYLKRTL
                     KDLQDLMDIHTDEHPNNYRLCKGIYVEPAEIAYKKYEEINQHFLEDLEFLLKNGNYVG
                     IATHDKPIIEGAYKLIEKYNVPKNKYEFQMLYGVTPELRKSIVDKGHTMRVYVPFGKE
                     WFGYSTRRLKENPKMASHIIKALFFRG"
     gene            complement(1178..1918)
                     /locus_tag="PLW22_11655"
     CDS             complement(1178..1918)
                     /locus_tag="PLW22_11655"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU75400.1"
                     /translation="MKKSDLAIISIGVVIAFGLCGYLIGQSIENFRNGDRTILVKGFA
                     EREVKADLAVWSISCRAASNDLVEGAKRIEQDQKKIIDFLVDKGIGKEEIISREIRVN
                     DRLANEYGGYDNVNFRYVIVKVLQVRSTNVDRVQEVSRLTDELVRAGVVISQNDYSNN
                     LQYYFTKLNDIKPEMLTEATQNAREAAISFASESGVKLGKLKRASQGLFTIVDRDQSL
                     SGGDSFASGNSDIVKRVRVVISVEYSIK"
     gene            complement(1977..3080)
                     /locus_tag="PLW22_11660"
     CDS             complement(1977..3080)
                     /locus_tag="PLW22_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU75401.1"
                     /translation="MQRLAHLLLAVLVLVLLSSWGGTGHYIISLEASRSFNTEMQQFA
                     DWANYLAGHASDADNRKSSDPTEAPKHYIDIDNYTEFFANQPMPLSLSEAVSKYGHSF
                     VVDNGILPWATKATYDSLVIALRNGRIDRAKLLAADLGHYVADGHMPLHLTKNYNGQL
                     TGNTGIHSRYESTMINAHADEFYNSQPREIVLVQNVQQYIFDYIYFNYQYVDSILEAD
                     NYARSINPDYSSSAYRDTLWVKTKGFTKRLFAAASQRLAELIYSAWVEAGRPSVTSAA
                     EVNLDRATMYVYPNPTSGQVNVTYSNSGGENYYLEIFDMLGNTLKRYSLNSISSSSGH
                     LTENLNFPSGAYLFRLSNNNISITQRVIVQKND"
BASE COUNT          881 a          777 c          670 g          967 t
ORIGIN      
        1 agatagggtt tttgtcatcc cgagcaaagt cgagggatct cttcaaagga ttgaatagaa
       61 atgagcaatg agatacttcg gcgaagtcta tcccaatata tcggggctca ggatggcatt
      121 tggtattgca ctactggtag ctaacaaaca tcctacactc aatgcgcagt ttgtacgata
      181 aggaaaaata aaagctccgg aaaacaccgg aacttttgaa aacaatatgc gttattatcc
      241 cctgaaaaag agtgccttga tgatatggct agccatttta ggattttcct tgagtcgacg
      301 ggttgagtaa ccgaaccact ccttgccaaa tggcacgtaa accctcatgg tgtgcccctt
      361 atctactatc gatttccgga gctcaggggt aacaccgtaa agcatctgga actcatactt
      421 attcttgggt acattatact tctcaatcag cttatacgct ccctcaataa ttggtttatc
      481 gtgggtagca atacctacat agttgccgtt tttcaatagg aattctagat cctcaaggaa
      541 atgctggtta atctcttcgt attttttgta ggcaatttca gccggctcaa cataaatccc
      601 cttgcaaagc ctatagttgt taggatgctc atcggtatgg atatccatta aatcctgcaa
      661 atccttaagc gtacgcttta ggtatgcctg gaataccaaa ccaacatttt tggggaactc
      721 ggcttttaac ctacggtaga gttcaatctc catgctggtg cagggcgagt cctccatatc
      781 gatacggatg aaattaccgt acgacgcagc cttagcaaca atttcacgga tatggttgta
      841 gcaaatctcc ttatcgatta acagcccaaa cgaggtgggt ttcaatgagt agttaccatc
      901 tatgttttcc ttctgaatgg tatcaataag ctccagatac tcctttttgt tacgttcggc
      961 ctcacctaaa tcctttatga attcacctag aatatcaatg gttaccttta tcccttgagc
     1021 attcagttcc ttggaaaccc taatggcatc ggcaatggtt tcgccggcaa tgtaacgttt
     1081 tgaaaaaatc cacaccagct tcttgggaaa gtacggaagc attgatgcaa tcaacttatt
     1141 gaacataaca attcaaaatt aatggttaaa cttatggtta ctttattgag tattcaacgc
     1201 taattactac cctaaccctt tttacaatat cgctgttacc tgaggcaaag ctatcgccac
     1261 ccgagagcga ctgatccctg tcgacaatgg taaacagccc ctggcttgcc cttttaagct
     1321 tgccaagctt aactccactc tcactggcaa aggagattgc tgcctccctg gcattctggg
     1381 tagcctcagt aagcatttcg ggcttaatat cgtttagctt ggtaaagtag tactggaggt
     1441 tattggagta atcgttctgg cttataacca caccggcacg gacaagctca tcggttaggc
     1501 ggcttacctc ctgtaccctg tccacattcg tgcttcgaac ctggagcacc ttaacaatca
     1561 catacctaaa gtttacattg tcgtagccgc catactcgtt ggctaacctg tcgttaacac
     1621 gaatttcgcg gctgataatc tcttccttgc caattccctt atccacaagg aaatcgatga
     1681 tcttcttctg atcctgctca atacgcttgg ccccttcaac caaatcgttg ctggccgcac
     1741 ggcagcttat gctccacact gccaaatcgg cttttacctc acgctcggca aaacctttta
     1801 ccagaatggt acggtcgcca ttcctaaagt tttcaatcga ctggcctatt aaatacccgc
     1861 atagcccaaa cgctataact actccgattg atattatcgc taaatccgat tttttcatgg
     1921 tcaatgcgtt tgcttaatgc aaagttagtt taataaccaa tgattaaacc atgatttcaa
     1981 tcattttttt ggactataac tctctgagta atgctaatgt tattgttgct caacctaaag
     2041 aggtaagccc ctgagggaaa attcaggttt tcggttaggt gacccgatga gctcgatatt
     2101 gaattcaaac tgtatcgttt taaagtattg ccaagcatat cgaaaatctc caggtaataa
     2161 ttttcaccgc ccgaattgct ataggtgaca tttacttgtc cgctggtggg attcggataa
     2221 acatacattg tagccctatc aaggttaacc tcggcagcgc ttgttaccga tggccttccg
     2281 gcctcaaccc acgcgctgta aatcagctca gccaatcgtt gcgatgcagc cgcaaataac
     2341 ctttttgtaa aaccttttgt ctttacccaa agagtatccc tgtatgccga tgagctgtaa
     2401 tcgggattga tgctcctggc atagttgtcg gcctccagta ttgaatctac gtactgatag
     2461 ttaaagtaga tgtaatcaaa tatgtattgc tgaacattct gaactaagac tatctcacgg
     2521 ggctgactat tatagaactc atctgcatgg gcgttaatca ttgttgactc atacctggag
     2581 tgtattccgg tgttgccggt aagctgacca ttgtagtttt tggtcagatg cagcggcata
     2641 tgcccatcgg ccacatagtg accaagatcg gcggccagca gcttagccct atcaatcctg
     2701 ccgttccgca aagcaataac cagtgaatcg taggttgctt tggttgccca gggcaatata
     2761 ccattgtcaa ccacaaagct atggccatac ttgcttacag cttcggacaa actcaggggc
     2821 atcggctggt tggcaaaaaa ttccgtatag ttatcaatat cgatgtaatg ctttggagcc
     2881 tcagtaggat cggaactttt acggttatcg gcatccgatg catggccggc caggtagttt
     2941 gcccaatcgg caaactgttg catttccgtg ttgaacgatc ggcttgcctc aaggctaatg
     3001 atgtaatgcc cggttccacc ccacgacgac aataaaacca aaaccaaaac tgcaagtaat
     3061 aaatgtgcta acctttgcat aatgctattt taaggttttg attgaatacg gacaatatgg
     3121 tttaacttgg tgaatactct ggcttgcgcg cgtctgtaaa ctctcgttcc cgctcgtttt
     3181 taaacgcgtg cggtattcac aactctgccc cattgctttc agtaaatgta gtaaaaaatt
     3241 cccttggttc aggctcgtac aaaaagcttt ttatgctctg gctgaggcgt agcgt
//
LOCUS       DAOOWJ010000183         3245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_29693_length_3245_cov_8.183699, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000183 DAOOWJ010000000
VERSION     DAOOWJ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29693_length_3245_cov_8.183699"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /gene="ssb"
                     /locus_tag="PLW22_11665"
     CDS             <1..387
                     /gene="ssb"
                     /locus_tag="PLW22_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU75402.1"
                     /translation="VRHLEGGATVARFSLATNETYTDKSGKKVTQTEWHNIVVWRGLA
                     EIAEKYVKSGKLLYVEGRIRNSSYEDKEGNKRYTTEILCDNFRFLGPSTGQGEKPNNY
                     NESHGTDVGAAESDAEIPQPDDDLPF"
     gene            complement(443..1822)
                     /gene="glmM"
                     /locus_tag="PLW22_11670"
     CDS             complement(443..1822)
                     /gene="glmM"
                     /locus_tag="PLW22_11670"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOU75403.1"
                     /translation="MTLIKSISGIRGTIGGRVGENLTPVDIVRFTAAYAEWLKQRNNG
                     KRYGVAVGRDARISGEMVNNLVIGTLMGCGIDVLNLGLATTPTVEMAVIHEKTDGGII
                     LTASHNPRQWNALKLLNEKGEFLSDTDGRMVLEIARSENFSFTDVEKLGAIHDEYSFV
                     NEHIEQIIKLKYVDVDAISNANFKVAIDCVNSVGGIALPRLLKALGVKNIVELYTDPT
                     GDFPHNPEPLPEHLTEISKVIQEQKADVGFVVDPDVDRLAIINEDGSMFGEEYTLVAC
                     ADYVLSQNPGNTVSNLSSTVALRDITHKYGKEYFASAVGEVNVVTKMKEVNAIIGGEG
                     NGGVILPELHYGRDALVGIALFLSHLAKSRMKCTELRRQYPSYFISKNKIELKQGLNP
                     DAVLERLKEKFSTEKINTIDGLRIDFAEGWVHMRKSNTEPIIRIYAEAQTAAKANELA
                     QQIIKEATK"
     gene            2183..>3245
                     /locus_tag="PLW22_11675"
     CDS             2183..>3245
                     /locus_tag="PLW22_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU75404.1"
                     /translation="MCRSKYIVVFILFLLAPGLNAQKVSLVLSGGGAKGLAHIGVIKA
                     LEENGIPIDNVSGTSMGAIIGGLYAMGYSPDEMVKLFKTRDFYNWSKGIIDESLKYNI
                     NDFSSSDAENLSVGLVLTSKGIKPKVASSFIPTVGMDIAFDELFSQGTALSGGDFNKL
                     FVPFRCNASDVVGKNIVYFRNGDLGRSIRTSMTFPLYYKPIYVDSLLLFDGGIYNNFI
                     WPEALKEFSPDFIIGSKVANNSKIPSDDDPFEQLEAMIVGMTNYDIPDSIGMVIDTRL
                     PNVNLWDFDRVDEIVGLGYQSAINLMDSIKAKVSRRVSLDSLAHARKQFREKLPPLYI
                     SQISVLGLNPKQQKYIDRVL"
BASE COUNT          862 a          698 c          717 g          968 t
ORIGIN      
        1 gttcgtcacc ttgaaggtgg agcaactgtt gcccgtttct ctttggcaac caatgagacc
       61 tacaccgata agtcgggtaa aaaggtaact caaaccgaat ggcacaacat tgttgtttgg
      121 cgtggtttgg ctgagattgc tgaaaagtat gttaaaagtg gtaaactcct ttacgttgaa
      181 gggcgcatac gtaattcctc gtacgaggac aaggagggta ataagcgtta taccactgag
      241 atactttgcg ataacttccg ctttcttggt ccaagcactg gtcagggaga aaaaccaaac
      301 aactacaatg aatcacatgg aaccgatgtg ggtgctgctg aaagcgatgc tgaaattccc
      361 caacccgacg atgatttacc attctaaaca acaagaaact ccgggaaaac cggagtttct
      421 tgtttgacag gatacctaaa ctttactttg tggcctcctt aattatttgt tgagcgagtt
      481 catttgcttt cgctgccgtt tgggcttcgg catatatccg aatgattggt tcggtgttcg
      541 atttgcgcat atgtacccat ccttccgcaa agtcgatacg caacccatcg atggtattga
      601 ttttctcggt tgagaatttt tccttaagtc gttctagaac ggcatcgggg tttaaaccct
      661 gcttaagttc tattttgttt tttgagatga aatagctagg gtattgccga cgaagttcag
      721 tgcatttcat tctgcttttg gcaaggtgcg aaaggaatag ggcaatgcca acaagggcgt
      781 cgcgtccata atgcagttct gggagtatta caccgccatt accctcgcca ccaataatgg
      841 cattcacctc tttcattttt gtaaccacgt taacctcgcc tacggctgat gcaaagtatt
      901 ccttgccgta cttatgggta atgtcgcgca gtgcaactgt gctcgacagg tttgaaacag
      961 tattgccagg gttttggcta agcacataat cggcgcacgc aaccagggtg tactcctccc
     1021 caaacatgct gccatcctcg ttaataatgg caagcctgtc cacatcgggg tcaaccacaa
     1081 agcccacatc ggctttttgt tcctgtatca cctttgatat ttcagttaaa tgttcaggca
     1141 gcggttcggg attatgtggg aaatcgccgg tggggtcggt gtaaagttca acaatgttct
     1201 ttacccccaa ggccttaagt aagcgcggta acgctattcc tccaaccgag ttcacacaat
     1261 cgatagccac cttgaagttt gcattactaa tggcatcaac atcaacgtat tttaatttga
     1321 tgatttgttc aatatgttcg tttacaaagg agtattcatc gtgaatagcg ccaagttttt
     1381 caacatcggt aaacgagaaa ttttcgctcc tggcaatttc aagaaccata cgcccgtcgg
     1441 tgtcggaaag aaactcgcct ttctcgttca gcagcttgag ggcgttccac tgacgtggat
     1501 tgtggcttgc agttagaata attccgccat cggttttctc gtggattacg gccatttcaa
     1561 cggtaggagt ggtggcaagc ccaaggttaa gaacatcgat accacatccc attaaggttc
     1621 caatcacaag gttgttgacc atctcgccgg atatgcgagc atcgcgacct acggcaactc
     1681 catatcgttt accattatta cgttgtttca gccactccgc ataggccgca gtaaacctta
     1741 caatgtcaac tggggtgaga ttttcaccaa cccttccgcc aattgtcccc cgtattcctg
     1801 atatcgattt aatgagagtc atctgttatt tgatttagtt acgcaaaggt agaaaaatag
     1861 tcgttgagaa aattttcatc aatcaactta attaaggaat cagaattcag acgtcaaaaa
     1921 tcagactata cctgataacg gctaacaatt aacaaatccc ttttcaccat ttgctttatg
     1981 cttttcagga ctattggtag taggtaggtt gttttttatc cagtttatcc tgacttattc
     2041 cgtctggtac ggtttcattc cttcttatgc tatgccaatt agctggagta ttaaattttg
     2101 ttagatattc cagaatatgt attctgatat tgtttagaca attaagtatt cctaatttta
     2161 gcttgacaaa tcgattacac ctatgtgccg ttcaaaatat atcgttgttt tcattctgtt
     2221 tctattagcg ccagggctta atgcccaaaa ggtgagcctg gttttaagcg gaggtggtgc
     2281 aaagggcttg gctcacattg gagttattaa agcacttgag gagaacggaa tccctatcga
     2341 caacgtgtcg ggaacatcaa tgggtgccat tattggtggg ctttacgcca tgggctattc
     2401 gcccgatgag atggttaaac tatttaaaac ccgcgatttt tacaattgga gtaaagggat
     2461 aattgacgaa tcgcttaagt ataacattaa cgatttctct tcaagtgatg ctgaaaatct
     2521 gagcgtagga ctcgtgctaa cttccaaggg aattaaacca aaggtggctt cgagttttat
     2581 acctacagtt gggatggata ttgcctttga tgagctcttt tcgcaaggca ccgccttatc
     2641 gggtggcgat tttaacaaac tctttgtgcc attcaggtgc aatgcctcgg atgtggttgg
     2701 taaaaacatt gtttacttcc gaaatggcga cttggggaga agtatcagga cttcaatgac
     2761 tttcccgctt tactataagc caatttacgt tgatagttta ctgcttttcg atggcggaat
     2821 ctataacaac ttcatctggc ctgaggcctt aaaggagttt tcgcccgatt tcattattgg
     2881 cagcaaggtg gctaataact ctaaaatccc ctcggacgac gatccgtttg aacagctcga
     2941 ggcaatgatt gtaggtatga ccaactatga tattcccgat tcaattggga tggttattga
     3001 tacccgtttg ccaaacgtta acctttggga ttttgacagg gttgatgaga ttgttggctt
     3061 aggttaccag tcggcgatta acttaatgga tagcattaag gcaaaggtaa gccgtagagt
     3121 atccctcgat agccttgctc atgcccgcaa acagtttcgg gagaaacttc ccccactata
     3181 catatcacaa atcagcgttt tgggattgaa ccccaagcag caaaagtata tcgacagagt
     3241 attaa
//
LOCUS       DAOOWJ010000184         3076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_31795_length_3076_cov_5.043694, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000184 DAOOWJ010000000
VERSION     DAOOWJ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31795_length_3076_cov_5.043694"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PLW22_11680"
     CDS             complement(<1..1078)
                     /locus_tag="PLW22_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU75405.1"
                     /translation="MHKIDKHPILEVPQRETVTFMFNGNKVKGEKGFTIAAALHQAGF
                     PVHSHSIENRERSLECGIGKCGACEMLVDGQIKRICITPVDGVKEVKEIPHEYTPDVK
                     PANKQESTKVFKTTVTIIGAGPAGLAAREILLEHGVENIVIDNNSTIGGQFNMQTHQF
                     FFFEREKKFGGMRGFEIASTLAGANHEGIFLNSTVWDILENKRIAIKNIKTEEIYYVE
                     SDYLVIATGAVPFMPTFENDDLPGVYTAAVVQKMMNQEFTLLGKNVLTVGAGNIGYLT
                     SYQLMQAGARVKAIIEAMPREGGFPVQANRVRRLGIPIMLGKILVKAIPNENHTGIIG
                     AVIADAKDFKPIPGTEQIIEGIDAI"
     gene            complement(1297..1767)
                     /locus_tag="PLW22_11685"
     CDS             complement(1297..1767)
                     /locus_tag="PLW22_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU75406.1"
                     /translation="MTIEIVKADKTNLIEVLYIIRECSRQLIEKGVKYWNNSLADRFE
                     ISTDIESGFIYLIKYNYVPVGTITLRFLPKKESLQLERLAIFPAYQKKGLGQKLIEFA
                     KETAKEKGVKKILGSIPIDDADLIHLLERNNFVKKGESIPVEMNTIALELELLQ"
     gene            complement(1764..>3076)
                     /locus_tag="PLW22_11690"
     CDS             complement(1764..>3076)
                     /locus_tag="PLW22_11690"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU75407.1"
                     /translation="EVVSRLVEHQNKYPTEWVEGRGWNQNEWDVKEFPTNELLNKAFP
                     DKPVYIVRIDGHAAIANSKALAIAGITGNTKISGGEIVLKDGKPTGILIDNAMELVRK
                     HIPTPSKKQKAQALLNAQQNCLAVGLTGVHDAGLDADEVELIDSMHKAGNLKMRINAM
                     LNPTHDNFEKYISKGIYSTERLMVRSIKIYADGALGSRGALLLKPYADDPSTRGIQIE
                     PTEKLDSVCSMAYKYGYQICTHCIGDAAVRLMLDIYSKYLPQGNDLRWRIEHSQVVDP
                     ADLPRYGEFDVIPSVQTTHATSDMLWAVNRLGDRVKYAYAYQNLLAQNGWLPNGSDFP
                     IEDINPLYGFYAAVARKNLYGEPQEGFQMENALTREQALRAMTIWAAKTCFEEEVKGS
                     IEAGKLADFVVLDLDLINSEISNALKAQVLQTFINGENVYTKNQ"
BASE COUNT          746 a          705 c          663 g          962 t
ORIGIN      
        1 ttatggcatc gataccctca atgatttgct cagtacctgg aatgggttta aagtccttgg
       61 cgtcggcaat aacagcccca ataattccgg tatggttctc gttgggaatc gccttaacca
      121 atattttgcc taacataata gggattccta atcgtctaac cctatttgcc tgaacaggga
      181 atccgccctc acgaggcata gcttcgataa tggcttttac acgagcacct gcctgcatta
      241 gctgatagct ggtgaggtag ccaatgttac cggctccaac agtgagcaca ttcttgccca
      301 gtagcgtaaa ttcctggttc atcatttttt gcaccactgc agcggtgtaa actcctggca
      361 ggtcatcgtt ctcaaaggtt ggcataaacg gaacggctcc cgtagcaatt accaggtaat
      421 cggattcaac gtagtaaatc tcctcggttt taatgttttt aatggcaatg cgtttgtttt
      481 caagtatatc ccaaacggta ctgttcagga aaataccttc gtggttagct cctgcaagcg
      541 tacttgcaat ttcaaagcct cgcatgccgc caaacttctt ttcacgttca aagaagaaga
      601 actggtgggt ttgcatgttg aactgcccgc caatggttga gttgttgtca attactatgt
      661 tttcaacacc atgttcaaga agtatttcac gggctgcaag tccagccgga ccagcgccaa
      721 taattgtaac agtggttttg aaaacctttg tgctttcctg tttgtttgct ggttttacat
      781 cgggagtgta ctcgtggggg atttccttaa cttcctttac cccatcgact ggagttatac
      841 agatacgctt aatttgtcca tcgacaagca tctcacaagc accacacttg ccaattccgc
      901 attcgaggct tcgttcacgg ttttcgatac tatgactgtg aaccggaaac ccagcttgat
      961 gtagcgctgc ggcaatggtg aatccttttt ctcctttaac cttattgcca ttaaacatga
     1021 aagtaacggt ttcgcgttga ggtacctcaa gaattgggtg tttatctatt ttatgcatgg
     1081 cttaaaatgc ttttattgtt acttggattg ccttagaaat ttttgcgaaa gtattttcca
     1141 agcgtgtgta atacaatgac taatgtcagg gaataaaaaa tagcaaccta atggttgcta
     1201 ttttttgata tcaaaatgtg aggaactgat gcgtttagct aatggaaaat ctaaggattt
     1261 ttaattgagg tgttggagga atttcccttt attgaactat tgcaatagtt caagttcaag
     1321 tgcaattgta ttcatttcaa ctgggatact ttcgcctttt ttcacaaaat tattccgttc
     1381 tagcaggtgg atcaggtcgg catcgtcaat gggaattgag ccaagtatct tttttactcc
     1441 cttttccttg gcagtttcct ttgcaaactc tatgagcttc tgaccaaggc ctttcttttg
     1501 gtatgctggg aatatggcaa gcctttcaag ctgtaaactc tcttttttag gtaaaaacct
     1561 taaggttatt gtgcctacag gtacatagtt gtactttata aggtagataa agcccgattc
     1621 aatgtctgtt gatatttcaa atctgtcggc caggctgttg ttccagtact taactccctt
     1681 ttctattagc tgcctggaac actcccgtat aatgtaaagt acctcaatta ggttggtttt
     1741 gtcagctttt acaatttcaa tcgtcattgg tttttcgtat atacattctc gccgttgata
     1801 aacgtctgca aaacttgtgc cttaagggca ttactaattt cggaattaat taaatcaagg
     1861 tcaagtacta caaaatcagc aagcttccca gcttcaatgc tacccttaac ctcttcttca
     1921 aagcaggtct tagcagccca aatggtcatg gctcggaggg cttgctcgcg ggttaaagcg
     1981 ttctccatct ggaatccctc ctggggttca ccgtaaaggt ttttacgcgc cacggcagcg
     2041 taaaaaccat atagcgggtt gatgtcctca atcgggaaat cgctgccgtt gggcagccag
     2101 ccgttttgcg ccagaaggtt ttggtaggcg taggcatatt ttaccctatc gcccagccgg
     2161 tttaccgccc acaacatgtc cgatgtggca tgggtggttt gaaccgatgg gattacatca
     2221 aactcgccat aacgcggcag atcggccgga tcaaccacct gcgagtgctc aatccgccag
     2281 cgtaggtcat tgccttgggg taggtatttg ctgtatatat ccagcatcag cctaaccgct
     2341 gcatcgccaa tacagtgcgt gcatatctgg tagccgtact tgtatgccat gctgcaaacc
     2401 gaatcaagtt tttcagtagg ctcaatttga atgccgcggg tactgggatc atcggcatag
     2461 ggtttgagta gtagtgcacc acgtgaaccc aatgcgccat cggcataaat ttttatcgac
     2521 ctaaccatca accgctctgt gctataaatt cccttgctga tatacttttc aaaattgtca
     2581 tgggtagggt tgagcatggc gttaatgcgc atttttaggt tccctgcttt atgcatcgag
     2641 tcgataagtt caacctcatc ggcatcgagc ccggcatcgt gaacgccagt tagcccaaca
     2701 gccaagcagt tttgctgagc atttagcaaa gcttgtgctt tttgtttctt acttggtgtg
     2761 ggaatatgct tgcgtaccag ctccatggca ttatcaatca gtatgcctgt aggcttaccg
     2821 tcctttaaaa ctatttcgcc acctgagatt tttgtattac ctgtaatacc tgctattgct
     2881 aatgctttgc tattagctat tgctgcatgc ccgtctatac gaacaatgta aactggcttg
     2941 tcggggaatg ccttgtttag cagttcgttg gtaggaaact ccttcacatc ccactcgttc
     3001 tggttccatc cccggccttc aacccattcc gttgggtatt tgttttggtg ctcaaccaga
     3061 cggctaacca cctcgc
//
LOCUS       DAOOWJ010000185         3062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_31989_length_3062_cov_8.989691, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000185 DAOOWJ010000000
VERSION     DAOOWJ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31989_length_3062_cov_8.989691"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2450)
                     /locus_tag="PLW22_11695"
     CDS             complement(<1..2450)
                     /locus_tag="PLW22_11695"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOU75408.1"
                     /translation="MHNIGNLEPILQGYFSVSEDKFEFSDKSCFVKGLSGSSLSFIVA
                     AMHGSSGKPTMLVFNEKEEAAYAFSDISTLLGMDRVFFFPSSYKRSIQFNQILSEAII
                     QRTDVLNLLRQMGDGENQKTVIVTYSDALFEKIPLRNILKENILTISKGERISTDFIT
                     EVLFEYGFERTDFVYEPGQFAIRGSIVDVFSYSSAMPYRIDFFGDEVESIRTFDVEDQ
                     LSRDLHQTISIIPNLQEKEQAEGAILTDELPENSTVWVKNPELVLERINQLNEIALQI
                     DFKAQAGYASGKEFFTSLQNHRIVYFGLRPPSTVHQNIEFSTSPQPAFSKNFELLADD
                     IEQKNLNGYRVYLLSENQAQLDRLNNILHSIKPDLHFELINESIAEGFVDHSLRICLY
                     TDHQIFDRYHKFRLKHEFTRRDALTMQELVNLQPGDYVVHIDHGIGVFGGLVKTEVNG
                     RLQEAIRLIYQDNDTLLVSIHNLHKISKYRGKDAEPPKVYKLGSGAWQKLKQRTKSKI
                     KDIAKDLIALYAKRKAQKGFAFSTDTYMQQELEASFIYEDTPDQQKATQAVKLDMEDE
                     RPMDRLVCGDVGFGKTEVAIRAAFKAVADSKQVAVLVPTTILALQHYRTFTSRLNGFP
                     CNVEFISRMKSAAQQREIRQRLKDGKIDILIGTHSILKEASNFKDLGLLIVDEEQKFG
                     VAAKEKIRQFKLNVDTLTLTATPIPRTLQFSLLGARDLSIINTPPPNRYPIQTELHVF
                     NTAIIKEAIEYEVSRGGQVFFVHNRVQNIGEVQIMLHKIVPKIKTAVAHGQLEPSNLE
                     SIMLDFINGDYDVLISTA"
BASE COUNT          863 a          689 c          620 g          890 t
ORIGIN      
        1 atagctgttg agatgagcac atcgtaatcg ccatttatga aatcgagcat aatactttca
       61 agattcgatg gctccaattg cccgtgagca acagccgttt taattttggg gactatcttg
      121 tgcagcatta tctgcacttc gccaatgttt tgaacccggt tatgcacaaa aaaaacctgc
      181 cctcccctgg aaacctcata ttcaattgct tcctttatta ttgctgtgtt aaaaacatgc
      241 aactcggttt gaatgggata tctattaggt ggcggggtgt tgattatcga taggtcccgg
      301 gctcccaaca gtgagaattg caaagttcgc ggaataggtg ttgccgttaa ggttaaagta
      361 tcgacattca gcttgaactg ccttattttt tccttagccg ctacgccaaa cttttgttct
      421 tcatcaacaa taagcagccc taaatcttta aagtttgatg cttctttaag tatggagtgt
      481 gtgccaataa gtatatctat tttaccgtct ttcaagcgtt gcctaatctc tcgctgttgt
      541 gcggctgatt tcatcctgct tataaactca acattgcagg gaaaaccatt gagcctcgat
      601 gtaaaggttc tgtaatgctg caatgccaga atggtagttg gtaccaaaac agccacctgc
      661 ttgctatcgg caactgcttt gaaagcggct ctaatagcca cctcggtttt accaaaacca
      721 acatcgccac acacaagcct atccattggg cgttcgtcct ccatgtcaag tttaacggcc
      781 tgggttgctt tctgctggtc aggggtatcc tcgtatataa atgaagcctc aagctcctgc
      841 tgcatgtagg tatcagttga gaaagcaaaa cccttctgtg cctttcgctt ggcataaagc
      901 gcaataaggt cttttgcaat gtcctttatt ttgctcttgg tgcgctgttt caacttttgc
      961 caagcccccg accctagctt gtaaaccttt ggtggctcag catccttacc gcgatacttg
     1021 gatattttat gaagattatg aatgcttaca agcaatgtgt cgttatcctg atagataagc
     1081 cgaattgcct cctgtaatct tccatttacc tcggttttta ccaaacctcc aaaaactcca
     1141 attccatgat cgatatgaac cacgtaatcg cccggttgca ggtttactag ttcctgcatg
     1201 gtgagcgcat cgcggcgggt gaattcgtgt tttaaacgga acttatggta acggtcaaag
     1261 atttggtgat cggtgtagag gcaaatcctc aagctgtggt caacaaaacc ctccgcaata
     1321 ctctcattaa ttagctcaaa atgcaagtcg ggcttaatgg aatgtaaaat gttgttgagc
     1381 ctgtcgagct gagcctgatt ctcggacagc aggtaaaccc tgtaaccatt taaatttttt
     1441 tgctcaatat catcggccag cagctcaaag ttttttgaga atgcaggctg aggtgaggtt
     1501 gaaaattcaa tgttttggtg aaccgttgag ggtggcctta aaccaaagta aactatccga
     1561 tggttctgaa gtgaggtaaa gaactccttg ccagaagcat atcccgcttg agccttaaaa
     1621 tcaatctgta atgctatctc gtttagctgg tttatccttt caagcaccaa ctcagggttc
     1681 tttacccaca ccgttgagtt ctcgggtaac tcatcggtta agatagcccc ttcagcttgc
     1741 tccttttcct gtaggttagg aattattgaa atggtttgat gtaagtcgcg agagagctgg
     1801 tcctccacat caaaggttcg tatggactca acctcatcac caaaaaaatc gatacggtaa
     1861 ggcattgccg acgagtacga gaaaacatca acaatgcttc ctcttatggc aaactgaccc
     1921 ggttcatata caaaatcggt tcgttcaaaa ccatactcaa aaagaacctc agtaatgaaa
     1981 tcggttgata tcctttctcc tttcgatatt gttaaaatat tctccttcaa aatattccta
     2041 aggggaattt tttcaaaaag cgcatcgcta taggttacta tgaccgtttt ttggttttca
     2101 ccatcaccca tttgccgcaa aaggttcaac acatcggtgc gttgaattat ggcctcagaa
     2161 agaatctgat tgaactgtat tgaccgcttg tatgaggacg ggaaaaagaa taccctgtcc
     2221 attccaagca aagtggaaat atcggaaaaa gcataagcgg cttcctcttt ctcgttaaaa
     2281 accaacatgg taggctttcc cgaggatccg tgcattgcag caacaataaa gcttaaggag
     2341 gaacccgata gtccctttac aaaacatgac ttatcggaaa actcaaattt gtcttccgat
     2401 actgagaaat atccttgtaa tattggttct agatttccta tgttgtgcaa tgtaaaattt
     2461 ttgcaaagat ataatgtgcc aatcaaaccg taagcaataa tcctaaaaca aatccatcag
     2521 gctgggagca aaattgttta atcgcttatt ttataagatg tatttcgcct aaccaacctc
     2581 aatagcaaag gaataacatt ccctaatatg atttttaatg acgattcaat gtcgtttagt
     2641 taacaaaaat attcatgcct gtcatcctga gcgtagcgaa ggatctctaa tcgacccatt
     2701 gagatgtttc gcttgcgctc aacatgacaa tagtttcaag aatagcgaat aacgcttcca
     2761 gcccggtagg ggcgtaatat ttgtaacaac atgattactc atacatgtat agcgatgcac
     2821 gcaatacata tgctaaagag caaccatgat acgttgcatc gcaactgaaa agcatggcaa
     2881 agagctattt atacgttctt tcttgtcttg atacaagaaa gaaccaaaga aaatcaaggc
     2941 tgaaaagccc gacccgatgg gtgccccgtg ggtggaactg ccacgcgata ctattcgcca
     3001 cgcctgcggc gtggctcatg tggtatcgct tactaagccc accgccacgt tccacccccg
     3061 ac
//
LOCUS       DAOOWJ010000186         3019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_32594_length_3019_cov_3.775978, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000186 DAOOWJ010000000
VERSION     DAOOWJ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32594_length_3019_cov_3.775978"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLW22_11700"
     CDS             <1..733
                     /locus_tag="PLW22_11700"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU75409.1"
                     /translation="EVDAVGTLRFLDAIKDTGLRTKFYQASTSELYGLVQEVPQTEKT
                     PFYPRSPYGVAKLYAYWITVNYREAYGLFACNGILFNHESPRRGETFVTRKITMAVAR
                     IKAGLQDKLFLGNLDARRDWGYAPEYVEGMWRMLQHSTPEDFVLATGKTYTVREFATL
                     TFKELGIDLEWQGKGVDEKGIDRATGKVLVEIDPNYFRPTEVDLLVGDSSKAKKLLGW
                     EPKVGIEELVQIMVKADWERVKGVI"
     gene            763..1856
                     /locus_tag="PLW22_11705"
                     /pseudo
     CDS             763..1856
                     /locus_tag="PLW22_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            1834..>3019
                     /locus_tag="PLW22_11710"
     CDS             1834..>3019
                     /locus_tag="PLW22_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU75410.1"
                     /translation="MITSMQGNRISIYRKLCLLNNQLIGWLIILIQFLVIEFSYSQTI
                     NIEQIKPLYRVAPVYGFKKGIVSLTFDDGYASQFGIGLRTLKRFHFPATFYVVSSFID
                     NAKALELKRDLPVEYEIGSHTSHHTHLTAIDNNDLLDEVSGSKYQLEHLFDRRVLTFA
                     YPWGDYNPKVKNTVENFYMAARSANLGYNWLDNLDVYGLKVQAFNQGINNRTAESWVD
                     FAINYRCWLIEMIHDIQGVGYKPLDSTEFVSHLEYINAKTSDLWVASVSDVIKYVNEA
                     KRINVSCISCSDSLYQLVVDDGLEDSIYDQELSIELRIPENWQQVNVSGAELVDFKFS
                     GGNNLARINVLPNAKPFTLKPNAFKQSIKPQNIKCVFNSENPFTDIISIAVYLPDLDD
                     ISV"
BASE COUNT          914 a          524 c          643 g          938 t
ORIGIN      
        1 cgaggttgat gccgttggaa cccttcgatt tctcgatgct atcaaggata caggtttaag
       61 aacaaaattt taccaggcct caaccagcga actttatgga ttagtacagg aagtgcccca
      121 aaccgaaaaa actccattct atccccgaag tccttacggt gtggctaaac tttatgcata
      181 ctggataacc gttaactacc gtgaagcata cggattattt gcctgtaatg ggatactttt
      241 caatcatgaa tcaccacggc gtggtgaaac ctttgttacc cgtaaaataa caatggctgt
      301 tgccagaatt aaagccggct tgcaggataa actattcctt gggaacctcg atgcacgccg
      361 cgactggggc tatgcccctg agtatgttga ggggatgtgg cgaatgcttc agcattcaac
      421 tcctgaggat tttgtacttg ccacaggtaa aacatatact gttcgtgaat ttgccaccct
      481 tacatttaag gagttaggta ttgaccttga gtggcaaggt aaaggtgttg atgagaaagg
      541 catcgatagg gctacaggaa aagttttggt tgagatcgat cctaactatt tccgcccaac
      601 cgaggttgat ttgctggttg gagattcgtc gaaagctaag aaattattgg gttgggagcc
      661 caaagttggt attgaagagt tagtgcaaat tatggttaag gctgattggg aaagggttaa
      721 aggagtcatt taatttcaca agaattagta gttaaacata aagtggaaaa aaactcaaaa
      781 atttatgtgg cgggtcattc cggtttggtt ggcaccgcca ttgttcgtaa attacaggaa
      841 aagggttact caaatattgt tacacgaacc cattccgaac ttgaattact caattacaat
      901 gaggtaattg atttttttac tactgaaagg cccgattatg tttttcttgc tgcagcccgg
      961 gttggtggaa tattggctaa caatacttat cgagccagtt tatttatgag aatcttcaaa
     1021 tacagagtaa tgtaatccat gcatcgtacc taagtcaagt taagaaactt ctatttttag
     1081 gtagttcatg tatttaccca cgcgaagctc ctcagcctat caaggaagag tatctcttaa
     1141 cttcgccttt ggagtatacc aatgaaccct atgctatagc aaaaatagca ggtataaaaa
     1201 tgtgcgagtc gtataacctt cagtatggca caaactttat ttcggttatg ccaactaacc
     1261 tttacgggcc aaacgataac tacgatttgg ccaagagcca tgttcttcct gcaattatac
     1321 gtaaaatgca tttggccaaa tgccttgaat gcaacgattg gactgcaatt cgtaacgatt
     1381 taaatagatt accaattgat ggggttaatg gagttgcctc agttgatgat attattgcag
     1441 tgctggcaaa atatggaata caaaaatcag ccaaaggtga tgtaaaagta actgttactc
     1501 tctggggctc aggcaaaccc ttaagggagt ttttacactc cagcgatatg gccgatgcct
     1561 gtgtttttat tatggaaaaa gtaaattttt ctgatttatc gagtggtatg atcgaagtgc
     1621 gaaatacgca cattaatatc ggtacaggac aggatatttc catagccgac ttgactcaat
     1681 tggtaaagga ggtggtaggt tttagtggtg gaattttgtg ggataaaact aaacctgacg
     1741 gaacgtatag gaaattgctc gacgtaagca agttgcactc actaggttgg aaccattcaa
     1801 ttgaacttac tcaaggagtt agaagggttt atcatgatta catcaatgca aggtaatcgt
     1861 atctcaatat atagaaaatt atgtctttta aataatcagc taattgggtg gttgattatt
     1921 ttgattcagt ttcttgttat agaattttca tattcgcaaa caattaatat tgaacaaatt
     1981 aaaccgctct acagggttgc acccgtatat ggttttaaaa agggtatagt aagtttaacc
     2041 ttcgacgatg gttatgcttc acagtttggt atagggctcc gtacgctcaa gcgtttccat
     2101 tttccagcaa ccttttatgt tgtttccagt tttattgata atgcaaaagc tctggagcta
     2161 aaaagagatt taccagtaga atatgagatt ggttcgcata cttctcatca cacacactta
     2221 actgcaattg ataataacga tttacttgat gaagtatcgg gatcaaagta tcaacttgaa
     2281 catctatttg accgtagggt tttgaccttt gcctacccgt ggggagatta caacccgaaa
     2341 gttaaaaata ctgtagaaaa tttttatatg gcggctcgtt cggccaatct tggttacaat
     2401 tggctcgata accttgatgt ttatgggctc aaggtgcagg cttttaatca gggaataaac
     2461 aaccggactg ctgagtcatg ggttgatttt gccattaact ataggtgttg gcttatagaa
     2521 atgattcatg acattcaagg cgttggctac aagcctctcg attcaactga atttgtatca
     2581 catttggagt atattaatgc taagacaagc gatttgtggg tagcatccgt ttcggatgta
     2641 ataaaatacg taaatgaggc taaaagaatc aatgtttctt gtattagctg ttccgattct
     2701 ttatatcagt tagttgttga tgatggttta gaagattcaa tttatgatca agaactttcc
     2761 atagaactcc gtattcctga gaattggcaa caagttaatg taagtggtgc agagttagtg
     2821 gattttaaat tcagtggtgg aaataatttg gcacggatta atgttttacc taatgcaaag
     2881 ccttttacct taaaacccaa tgccttcaaa caatcaataa aacctcaaaa tataaagtgt
     2941 gtttttaaca gtgaaaatcc ttttaccgat attattagta tagctgttta tcttcctgat
     3001 cttgatgata tcagcgtta
//
LOCUS       DAOOWJ010000187         3016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_32639_length_3016_cov_7.656873, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000187 DAOOWJ010000000
VERSION     DAOOWJ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32639_length_3016_cov_7.656873"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1251
                     /gene="porQ"
                     /locus_tag="PLW22_11715"
     CDS             217..1251
                     /gene="porQ"
                     /locus_tag="PLW22_11715"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOU75411.1"
                     /translation="MRFATTIILCFIMAISVNGQNGGLSTYQFLNLPYSSRVAAFGGK
                     MAASDLADISLTNQNPAMLDSSLHNAVSLFYTSYYASINFAAVSYAQRLNSRSTLGFS
                     IFGINYGRFDQADESGAISGTFGGNDFALAVTYSYRIDSCFNVGVSVKPIYSHLERYY
                     SLGISTDFGVHYLSGNKLFAAGIALRNVGVMVKPYTANTYENLPFEAVAGFSKKLAHA
                     PFRFVVTMQHLERYDLYYSSPINKTDILGEDDKPSPNVVERVGRELISHLIAGIEFVP
                     VKGFALRLGYNYQRRNELKVQERVSTVGFSWGVSLRIKKFDVSYSRATYHLTGSTNHF
                     SITTNLQNWL"
     gene            1314..2003
                     /gene="cmk"
                     /locus_tag="PLW22_11720"
     CDS             1314..2003
                     /gene="cmk"
                     /locus_tag="PLW22_11720"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOU75412.1"
                     /translation="MDKKIVIAIDGFSSCGKSTFAKAIAKRLGYLYIDSGAMYRVVTL
                     ELLRRGLINDGVVDTSMLQSILKEINIGFSYDPVKMAYLTTLNGEVVEDEIRSPQVAN
                     HVSLVAAIPAVRTRLVELQRELGKKKGIVMDGRDIGTVVFPDAELKIFMTADPIVRAR
                     RRLKELQEKGLNVTLDEVLKNIEERDYIDQNRDVAPLRKADDAVVLDNTNMTVEEQME
                     WVEEMVKRLTV"
     gene            2049..2936
                     /locus_tag="PLW22_11725"
     CDS             2049..2936
                     /locus_tag="PLW22_11725"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOU75413.1"
                     /translation="MRIEIDPNAGFCFGVVNAIKTAEENLSKGIEINCLGEIVHNEME
                     NNRLKGIGMKTVKHDQLPELGGRSVLIRAHGEPPSTYELAKKYNINLVDATCPVVLKL
                     QERVRKAWERLQPLGGTVIIFGKKGHAEVIGLCGQTNNQAVVVQDLNDLDGIDFSKPI
                     EIFSQTTKEPEHYREIVEEIKRRVAIYNTQPETLLKVNNTICGQVSNRKPHIKAFAQK
                     HDLMIFVSGTNSSNGKMLFEACLEVNPNSHLIVNSAELKPEWLSGCQSVGISGATSTP
                     LWLMQEVASAIEAIVKGKG"
BASE COUNT          842 a          625 c          739 g          810 t
ORIGIN      
        1 cttgaggttg atggcgaatc gcttggcgaa actcctctta aattctcaat tatgcagcaa
       61 gcgctctccg ttatagttgg tgagcagttt aaccagtcca agctaaccgc taagaaacag
      121 ccccagctac ttgaaccggc agccaggtag gcaatatttg gcatatacta tccgtttaga
      181 atgatattta attagatttt agcccataaa accaccatgc ggtttgcaac aacaatcatt
      241 ctttgtttca ttatggccat ttccgttaat ggtcagaacg ggggcttatc tacctatcag
      301 tttctgaatc tgccttactc ttcgcgcgtt gctgcctttg gtggtaagat ggcagcatcg
      361 gatttggccg atatctccct gactaaccaa aatccggcta tgctcgattc atcactgcat
      421 aatgccgtta gcttgttcta cacaagctac tacgccagca ttaattttgc agcggtgtcg
      481 tacgcccagc gattgaactc cagaagtacg cttggctttt caatttttgg aattaactat
      541 ggccgttttg accaagccga tgaatcggga gcaatatcgg gcacctttgg cggcaacgat
      601 tttgctttgg cggtaaccta cagctaccgt atcgattcct gttttaatgt aggggtaagc
      661 gttaaaccca tctactcgca cttggagcgt tattactccc tggggatttc taccgatttt
      721 ggtgtacatt acctttcagg caataaactc tttgctgcgg gtattgcgct taggaatgtt
      781 ggtgtgatgg taaaacctta cactgccaat acttacgaga acttaccttt tgaggctgtt
      841 gccggtttca gtaagaagtt ggcgcatgca cctttcaggt ttgttgtaac catgcagcac
      901 ctggagcgtt acgatttgta ttacagttcc cccattaaca aaaccgatat tcttggcgaa
      961 gatgacaaac cgtcgcccaa tgtggttgag cgggtaggcc gtgagttgat tagccatttg
     1021 attgccggga tagagtttgt acccgtgaaa ggctttgcct tacgtctagg gtataactac
     1081 cagcgtcgga atgaacttaa ggtgcaggag cgagtttcaa ccgttgggtt tagctgggga
     1141 gtaagcctta gaataaagaa gtttgatgtt agctacagta gggcaacata ccatttaacc
     1201 ggctctacca accatttttc aataaccacc aatcttcaaa actggcttta gaggccaccc
     1261 atcaagtgga gtttttatta ccttagcggg ttcaaaaaat gatcttacaa ccaatggata
     1321 aaaaaatagt tattgctatt gatggattct cgtcgtgtgg gaaaagcacg tttgctaagg
     1381 ccattgcaaa acgtttagga tacctgtaca ttgatagcgg agccatgtat cgcgtggtta
     1441 cgcttgaact gctccgccgg gggcttatta acgatggcgt ggtggataca tcaatgcttc
     1501 aaagtatttt aaaggagata aacattggct tctcatacga tcctgttaaa atggcttacc
     1561 ttacaaccct gaatggcgag gtggttgagg acgaaattcg gagtccgcag gttgctaacc
     1621 atgtgagcct tgttgccgct ataccggctg tgcgaacccg actagtggag ttgcaaaggg
     1681 aacttggcaa aaagaaagga attgtaatgg atggccgcga tattggtaca gtggtattcc
     1741 ccgatgctga gctgaaaata ttcatgacag ccgaccctat tgttcgtgcc cggcgcaggt
     1801 taaaggagtt gcaggaaaag ggtttgaatg ttaccttaga cgaggtgctt aaaaatattg
     1861 aggagcgcga ttacattgac cagaaccgcg atgtggctcc actccgcaaa gccgatgatg
     1921 ccgtggttct cgataatact aacatgacag ttgaggagca aatggagtgg gtggaggaaa
     1981 tggttaagcg ccttaccgtt taaaccaaat tatagcttat ctgtttagca gtataatact
     2041 gaatttgaat gagaattgag attgatccta atgccggttt ctgttttggt gtagtaaatg
     2101 caatcaaaac agccgaagag aatctgagca aaggaattga aatcaactgt ctgggcgaga
     2161 ttgtccataa cgagatggaa aataatcgcc ttaaagggat aggcatgaaa accgtaaagc
     2221 acgaccagtt gcccgagttg ggagggagga gtgtcctaat ccgtgctcac ggtgaaccac
     2281 catcaaccta tgagttagca aaaaagtaca acatcaacct ggttgatgct acctgtccgg
     2341 tggttcttaa gctgcaggaa cgtgtccgca aggcctggga acgccttcaa ccccttggcg
     2401 gaactgtaat tatttttggt aaaaaggggc acgctgaggt tatcggccta tgcggacaaa
     2461 ctaacaacca ggctgtagta gttcaggatt tgaacgatct tgacgggatc gatttcagta
     2521 aacctatcga aattttctcg caaaccacaa aggaacccga gcactatagg gagattgttg
     2581 aggagataaa gcgccgggta gctatataca acacacagcc cgaaacccta cttaaggtta
     2641 ataataccat ttgcggacag gtgtcgaacc ggaagcctca cattaaggct tttgcacaaa
     2701 aacacgattt gatgattttt gttagtggca ccaacagctc aaacgggaag atgctttttg
     2761 aggcctgcct ggaagtaaac cccaatagcc atttgattgt gaattcagct gaattaaagc
     2821 cagaatggtt aagtggttgc cagagtgtag gtattagcgg ggcaacctca accccacttt
     2881 ggctcatgca ggaggtggca agtgccattg aggcaattgt taaggggaaa ggataaaggt
     2941 taaaggataa aggttaaagg ataaaggttg acccgtcctg aaaaatgtct acacaaactt
     3001 atgtgtatgg acaaaa
//
LOCUS       DAOOWJ010000188         2961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_33430_length_2961_cov_4.397798, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000188 DAOOWJ010000000
VERSION     DAOOWJ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33430_length_2961_cov_4.397798"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..651
                     /locus_tag="PLW22_11730"
     CDS             271..651
                     /locus_tag="PLW22_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75414.1"
                     /translation="MKSIVKIVGISVMAILMVSFSANAQSVVKVKPAHKTVFVKQKGP
                     RGKKVVVYHPRWAPKKAFYRRWVYFPAYNLYWDNVRELYVYRKGNVWVTSVTVPAFVI
                     NVDLSTAKHEELPEEKDSQDVPEK"
     gene            complement(722..2413)
                     /locus_tag="PLW22_11735"
     CDS             complement(722..2413)
                     /locus_tag="PLW22_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75415.1"
                     /translation="MNRFAQILLVTLTGFTFGSFAQDAEKKTFYIDSTGNLYVPLSQP
                     VQIYMGTEPDGSNAVLLKSINKISAPLTWNGSGPQLMSHLDLYKGRKILFELYADGIP
                     PQTSINPKSQNILEQKDAIIIRGGSIIELNATDEAAGVDKILYSINGSSFAPYNTPIN
                     LTDDGEYEIKVYSTDKLGNSEPVVSRKIIVDATPPLTEISFKGDKYEDIFSGRAALTL
                     TASDRFGVAGTKVMIDSSGKWTDYKQPIQTANLAEGYHTLTWYSTDRAGNIEVQKNYR
                     FFVDKTPPIVVEEIEGNSYMIGTREFSSGRSRLKVMAVDNKAGVKEIYYSLNNREFKL
                     YEKPVMLADILGTVKLKTYAIDRVNNRSNSEANAQSFTMPTIDITGPELGYRLNGPKL
                     VLRDTLWIGPETKIELLATDREAGVNRIEYSLNQAQNSQYFEPFVITENGYYRLEFTG
                     YDNVENVNVSGFSFAVDTQAPEIFWHFSVKPYATVTVDGEELPVFPPNVKIFLGATDN
                     VTPLVKLTYSWNGNKPQEYNKPIIIPNIQPVNTLDIIATDEIGNTIQQSLRFAVK"
     gene            complement(2422..>2961)
                     /locus_tag="PLW22_11740"
     CDS             complement(2422..>2961)
                     /locus_tag="PLW22_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU75416.1"
                     /translation="DSKSGVKQSFIAVDNQPFAPFNGNVDIERTGEYTIKVYSTDNVG
                     NVGDTVVYKIVAAPDVTFKIDNIYFETASARITGNSINNLKEMVEILKNYPELHVEIK
                     AHADSRGSSEYNLELSQRRAQSVVNYLTSRGIEGYRLKDKGYGDTQPVNECVKGIKCP
                     DSKLRENRRVEFRFYLPKK"
BASE COUNT          792 a          570 c          666 g          933 t
ORIGIN      
        1 cgtatataaa tcaaataaac aaagttgctg aggagcaagg tatccatgcc tttgtttggg
       61 atattgagag gatggtttaa cctaaagcat tattgtcaaa tgacaaagag ccgaacgtta
      121 tattgttcgg ctcttagctt tattagattt agtagactga gggataaaaa acactttacc
      181 tattgtatag gagaagtaaa tttaattttt ttttggtagg gtaattttga agtaagtttt
      241 actatataca gaaactaaac aattacatgt atgaaaagca tagttaaaat agtaggcata
      301 tcggtaatgg ccatactgat ggtatcattt tcagctaacg cacaaagtgt ggtaaaggtt
      361 aaacctgccc ataaaacagt atttgtaaaa cagaagggac cgcgagggaa aaaggttgtg
      421 gtttatcatc cgcgctgggc tccaaaaaaa gcgttctatc gtcgttgggt ttacttcccg
      481 gcttataacc tttactggga taatgtgagg gagctatacg tttatcgcaa gggcaatgtt
      541 tgggtaacat cggttaccgt acccgctttt gtgataaatg tagacctaag cactgcaaaa
      601 cacgaggaat tacctgagga aaaggatagt caggacgtac ccgaaaagta gtacatgggg
      661 tagtaaagat aaagggcttg aaaaatcaag cccttcttat tatctataat ctggtattgg
      721 attactttac agcgaaacgt aaactttgtt ggattgtgtt cccaatttcg tcggttgcaa
      781 ttatatcaag tgtgtttaca ggttgaatat ttggaataat aataggttta ttatactcct
      841 gtggtttatt tccgttccat gagtaggtaa gttttaccaa aggcgttaca ttatccgttg
      901 cacccaagaa aatctttaca ttagggggaa atacaggcag ctcttcgcca tcaactgtaa
      961 ctgtggcata gggtttcaca ctgaaatgcc agaatatttc aggggcttgg gtatcaactg
     1021 caaaggagaa gcccgaaacg ttcacgtttt ccacattgtc gtaccctgta aattcaagcc
     1081 tgtagtatcc gttctcggtt ataacgaatg gttcaaagta ttggctgttt tgtgcttgat
     1141 tcagtgagta ctcaatacgg tttacgccag cttccctatc ggttgcaagg agctcaattt
     1201 tggtctcagg ccctatccaa agcgtgtcgc gcaatacaag tttagggcca tttaggcgat
     1261 agccaagttc gggccctgtt atatcgattg tgggcatggt gaagctctga gcgtttgcct
     1321 cggagttact gcggttattt accctatcga tagcgtaagt ttttagtttt accgtaccca
     1381 agatatcggc aagcattacc ggtttttcgt agagcttgaa ctccctgttg ttcagggagt
     1441 agtaaatttc ctttacccct gctttgttgt caacggccat tacttttaaa cggctacgtc
     1501 ccgatgagaa ctcacgggtg ccaatcatgt atgagttccc ttcaatttcc tcaactacaa
     1561 tgggtggggt tttatcaaca aagaatctgt agttcttttg cacttcaata ttccctgctc
     1621 tatcggttga gtaccaggtt agggtatggt atccctcagc caggttagca gtttgtatgg
     1681 gttgtttata atcagtccac ttgcccgatg aatcaatcat aaccttagtg cctgctacac
     1741 caaacctgtc gctagctgta agagtaagcg cagccctacc tgaaaaaatg tcttcgtact
     1801 tatcaccctt aaatgagatt tcggttaagg gtggggtggc atccacaata atttttcggg
     1861 atactactgg ctcactgtta ccaagcttat cggttgagta taccttaatc tcgtattcgc
     1921 catcatcggt gaggttaata ggggtgttgt acggagcaaa actgcttcca ttaattgagt
     1981 agagtatttt atcaacaccg gccgcttcat cggtagcgtt gagttcaata attgagcccc
     2041 cccggataat aatcgcatct ttttgctcta gaatattctg actcttgggg tttatggatg
     2101 tttggggtgg gataccgtcg gcgtaaagtt caaataggat tttccgacct ttgtaaaggt
     2161 caaggtgcga cattagttgt ggacccgaac cattccaggt taaaggggct gatattttat
     2221 tgatactttt tagcaatact gcattggagc catcgggttc tgtgcccatg tatatttgaa
     2281 caggttggct gagcggaacg taaagattcc cggttgaatc aatataaaat gtttttttct
     2341 cggcgtcttg ggcgaaacta ccaaaagtaa agccggttag ggtcacaaga agtatttgag
     2401 caaacctatt catactcatg gttatttttt tggtaagtaa aagcggaatt ccacgcgacg
     2461 gttttcacgg agcttgctat cggggcattt tattccctta acacactcgt taaccggttg
     2521 ggtatcgcca tagcctttat cttttaaacg gtaaccttct attcctcgcg atgtgaggta
     2581 gtttaccact gactgggccc ggcgttgcga taattcaagg ttatactcgc ttgaaccgcg
     2641 gctatcggca tgggctttaa tttcaacatg cagttcaggg tagtttttta gtatctctac
     2701 catttctttc aggttgttga tagagtttcc tgtaatccgt gcgcttgcgg tttcaaagta
     2761 gatattatca attttaaagg ttacatcggg ggctgcaaca attttataaa cgactgtatc
     2821 gccaacgttg cctacattgt cggttgagta aacctttatg gtgtactcgc cggttcgttc
     2881 tatatcaaca tttccattaa aaggtgcaaa cggttggtta tcaacggcaa taaatgattg
     2941 ctttacaccc gattttgaat c
//
LOCUS       DAOOWJ010000189         2919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_34031_length_2919_cov_6.626397, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000189 DAOOWJ010000000
VERSION     DAOOWJ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34031_length_2919_cov_6.626397"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..1635
                     /locus_tag="PLW22_11745"
     CDS             367..1635
                     /locus_tag="PLW22_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOU75417.1"
                     /translation="MNLKLRLTLMNFLQFFIWGSWLLTIGAYWFQTKQWSGTEFGAIF
                     STMGLASLFMPAIAGIIADRWINAERLYGIFHILGGVMLFIVPMVNNPTTMFWVMLVN
                     MMFYMPTIALSITVAYSSMKSRGMDIIKEYPPIRVWGTIGFIVALWFISLMGFETSST
                     QFYVASMASFILGLYAFTLPQCPPLARGHKKSLVDELGLNAFKLFKSTKMAIFFIFAM
                     LLGASLQLTNAYGDTFLHDFAKLPQFADLVAVKYPAIIMSISQISETLFILTIPFFLK
                     RFGIKNVMLMSMVAWVLRFGLFAVGDPGPGLWMIILSCIIYGMAFDFFNISGSLFVET
                     QSEPSIRASAQGLFMMMTNGFGAFLGSSISGIIIDKFFTIPNAPEGAIQFNWTGIWIT
                     FAAYSLVVAVLFAFLFKHKHNPADVANVQH"
     gene            complement(1704..2312)
                     /locus_tag="PLW22_11750"
     CDS             complement(1704..2312)
                     /locus_tag="PLW22_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOU75418.1"
                     /translation="MSKIKLTVLGISYSQTQSGAYALVLSEENGNRRIPIIIGGYEAQ
                     AIAIQLEGLTPPRPLTHDLFLNFARAFGIDLIDVHIYKLEDGVFFSKLHCEDSKKELF
                     IDSRTSDAVALALRFNCPIYTTEEIIEKAGITLDVEEYNSEEPQDESETETPEPTDSF
                     TDQIRRLSLDELNRLLSEAVENEEYEKATKIRDEIKRREKKK"
BASE COUNT          828 a          581 c          633 g          877 t
ORIGIN      
        1 taaaggataa aggttaaagg ataaaggtta aaggataaag gttaaaggat aaagggtaaa
       61 ggataaagga taaaggataa agggtgaccc gtcctgaaat aagtcaccgg gttaatgagt
      121 aggttcaggg cttgtaaata aaacatcatt agggtttata gagtaggata ataaatggtt
      181 tctgcagaac ttctatgttt tggtttcagt aagtcaacat ttccctgatt tctgtaaaat
      241 ggtagaaaat atgctctgaa aagttcaaat gtttaggtta agtatctaaa aaattgttga
      301 ctatatagta taaccaaaaa aacgatacta tttttaccga ccaaatcaac aactaaattg
      361 aacattatga atttgaaact acgacttacc ctaatgaatt tccttcagtt tttcatttgg
      421 ggttcatggt tacttaccat aggggcttac tggtttcaaa ccaagcagtg gagcggaaca
      481 gaatttggcg ccattttctc cacaatggga ttggcatcgc tgttcatgcc tgccatagca
      541 ggtatcattg ccgaccgttg gataaatgcc gaaaggcttt acggaatttt ccacatactt
      601 ggtggagtaa tgctatttat agtcccaatg gtcaacaacc ccacaacaat gttttgggtt
      661 atgctggtta acatgatgtt ttacatgcca accattgctc tttccatcac ggttgcttac
      721 tcctcaatga aaagcagggg aatggatatc ataaaagagt atccgccaat aagggtgtgg
      781 ggaacaattg gttttattgt ggcattatgg tttatcagct taatgggctt tgaaacgtca
      841 tcaacccagt tttacgttgc ttccatggca tcgttcattt taggcctata cgcctttacc
      901 ttacctcaat gcccgccctt agctcgtggt cataagaagt cgttggttga tgagcttgga
      961 ctaaatgcgt ttaagctttt caagagcacc aagatggcca ttttcttcat ttttgccatg
     1021 ttgcttggcg catcgctgca gctgacaaac gcctacggcg acaccttcct tcacgatttt
     1081 gccaaactac cccagtttgc tgatctggtt gcagtaaaat accctgccat tatcatgtcc
     1141 atttcacaga tctccgaaac tctgtttatt cttaccattc ccttcttcct taagcgtttt
     1201 gggattaaaa atgtgatgct gatgagcatg gtagcatggg tgctgcgttt tggccttttt
     1261 gcagttggcg atccaggccc gggtctttgg atgattatcc tttcatgtat aatctacggt
     1321 atggctttcg atttcttcaa catatcaggt tcgttgtttg ttgaaaccca aagtgagcca
     1381 tccataaggg caagtgcgca aggtttgttt atgatgatga caaacggttt tggtgctttt
     1441 ctgggaagta gcattagcgg tatcataatc gacaagttct tcaccatacc taatgcgcct
     1501 gaaggtgcaa tacagttcaa ctggacaggc atttggataa cctttgcagc ctactcgcta
     1561 gtggttgccg ttctgttcgc tttcctattc aagcataagc ataaccctgc cgatgtggct
     1621 aacgttcagc actaaaaggt gggtgtgtaa aaatataaaa ggccatggga aaaactcatg
     1681 gcctttgttt ttgggagatt ttattacttt ttcttttctc tacgttttat ctcgtcgcga
     1741 attttggttg ctttttcgta ctcctcgttt tcaacagcct cactaagtaa gcggttgagc
     1801 tcatcgagcg ataacctgcg gatttgatca gtaaagctgt cggttggttc tggtgtttca
     1861 gtttcgctct catcttgagg ttcttcggaa ttatactcct ctacatcgag ggtgatacct
     1921 gccttttcaa ttatctcctc agtggtataa atggggcagt tgaacctaag ggcaagggca
     1981 acagcatcgg aagtgcgcga gtcaataaag agttcctttt tgctatcctc acaatgcagt
     2041 tttgaaaaga aaacgccatc ttccagctta tagatgtgaa cgtcaataag atcgatacca
     2101 aaggcacggg caaagttaag gaataggtcg tgggttagcg ggcggggcgg ggttagccct
     2161 tcgagctgaa tggctatggc ttgggcctcg taccccccta tgattatggg aattcgtcgg
     2221 ttcccattct cctcgctcag aaccagagcg tatgctcctg attgggtttg actatacgat
     2281 atccctaata cggttaactt aattttactc atttgaacaa atgttcagtg tgtagaaaca
     2341 gatttgcaaa ttaaaacaaa tataccattt attcacaaac ccaaacaatt ttttgagttg
     2401 ataattaaat gttgattagt taacaaaaac aagcattcct tttgtcttcc tgagcgaagc
     2461 gaaggaactc attattagcg taccgtgttc agtgagcagt gtacagtagg ggggtgatgt
     2521 ttaaggataa aggataaagg ataaggttca aggtttaagg ttcaaagttc aaggttcatg
     2581 taaaaggcat aatgtcatag gcttaggatt tattatcaac tatcaaccat caactaaata
     2641 ggtatcacag agttacacag tgtaacacgg tgttacacag aatggcattc acgaacaacg
     2701 aatcacgatc aacgaacaac gaacgccgtc cccagccccg taggggcgaa atatttgtaa
     2761 ccccatggct actcccacat gtatagcgat gcacgcaatg ccttttccaa agaacaacac
     2821 tgaatcattg catcgcaaag gaaaagcatg gcaaagagcc attcacacgt tctttcttgt
     2881 cttgatacaa gaaagaacca aagaagatca aggctgaaa
//
LOCUS       DAOOWJ010000190         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_35012_length_2858_cov_4.493043, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000190 DAOOWJ010000000
VERSION     DAOOWJ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35012_length_2858_cov_4.493043"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PLW22_11755"
     CDS             complement(<1..967)
                     /locus_tag="PLW22_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOU75419.1"
                     /translation="MNTELIIDVRETEVAIALLENKQLVELNKERSNVQFSVGDIYLG
                     KVKKIMPGLNAAFVDVGYEKDAFLHYLDLGPQFKTLQKYVQDHYSRKNKTIPISKYGM
                     LPDIDKSGKITDVLSTGQPVMVQIAKEPISTKGPRLTSEISIAGRNIVLVPFNDKVSI
                     SQKITAPEERKRLKRLMESIIPKNFGVIVRTAAEGKKVAVLDAELRSLIKRWEITVEK
                     LKNPGYPVLLTSEIKRTSAILRDMLNGSFNSIHVNDENVYEEIREYIESIAPEKEKIV
                     KLYNGNVPIFDQFGVEKQIKSLFGRTVSIKSGAYLIVEHTEALHVI"
     gene            complement(1191..1478)
                     /locus_tag="PLW22_11760"
     CDS             complement(1191..1478)
                     /locus_tag="PLW22_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU75420.1"
                     /translation="MTKADIVNEISKSTGIEKVAVQKTVEAFMEAVKDSLVKEKNVYL
                     RGFGSFIVKKRAKKTARNISKNTTIIIPEHFIPAFKPSKSFVNKVKNHVKK"
     gene            1581..2690
                     /gene="mutY"
                     /locus_tag="PLW22_11765"
     CDS             1581..2690
                     /gene="mutY"
                     /locus_tag="PLW22_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOU75421.1"
                     /translation="MTRFFFFLFDSMDFSSILFKWYSTKARDLPWRNTINPYHIWVSE
                     VILQQTRVVQGISYYYRFIERFPSIQSLADADIDDVMKVWQGLGYYTRARNLKAGAIQ
                     VMEQYNGNLPKSYKELQRIKGLGAYSAAAIASFAFGEAVAAVDGNVYRILSRIFGIFT
                     PIDSSTGKKDFFALANELIPKNEPGRFNQALIDFGALQCTPKSPGCTDCPFADYCYAY
                     KNNLIVALPVKSKKKPPVERFFYYFMIKHKGHTFIKRRQEKDIWHSLYEFPMVETGAK
                     LGHDQLLERMDSLNFLSGSTVNVLSISQPIRHLLSHRTIWATFIILEVDNPNYILTEE
                     YVRIPIGGVSDYSLPRLIDIYMAAEPVIKYFSSKK"
     gene            2753..>2858
                     /locus_tag="PLW22_11770"
     CDS             2753..>2858
                     /locus_tag="PLW22_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU75422.1"
                     /translation="MSVNKVILVGNVGKDPEVRHLEGGATVARFSLATN"
BASE COUNT          749 a          585 c          590 g          934 t
ORIGIN      
        1 caatcacatg aagcgcctca gtatgctcaa caatgaggta tgccccgctt ttaattgaaa
       61 ccgtccggcc aaatagcgat ttaatttgct tttcaacacc aaactggtca aatatgggca
      121 catttccgtt gtaaagcttt acaatttttt ctttttcggg tgcaatgctc tcaatgtact
      181 cgcgtatttc ttcgtaaacg ttctcgtcgt tcacatgaat gctgttgaac gaaccgttaa
      241 gcatatccct caggatagcc gatgttcgtt taatttcgct ggtaagcagt accggataac
      301 ccggattctt aagtttttca acggtgattt cccacctttt aattaagctg cgcagctctg
      361 catcgagaac tgcaaccttt ttgccctcgg cggcggtacg gacaataacc ccaaaattct
      421 ttggaataat actttccatt agccttttta gtcgcttgcg ctcctcgggg gcagtaattt
      481 tttgcgagat tgaaacctta tcgttaaagg gtacaagaac gatattccgg ccggcaattg
      541 atatttcaga ggtgagcctt ggccctttgg ttgaaatggg ctccttggca atttgcacca
      601 ttaccggctg tccggtgcta agcacatcgg ttattttccc actcttgtca atgtcgggca
      661 acatcccgta ctttgagatg ggaatggttt tgtttttacg cgagtagtga tcctgaacgt
      721 acttctggag tgttttaaac tgtggcccaa ggtcgaggta atgaaggaat gcgtcctttt
      781 cgtagcccac atctacaaag gctgcattta acccgggcat aatttttttt actttaccga
      841 gatagatgtc gccaaccgag aactgaacgt tgctccgctc cttgttgagc tccaccagct
      901 gcttgttttc gagcagggca atcgcaactt cagtttcacg aacatcgatg attaactcgg
      961 tattcacttt ttacgtgtat tttgtggtta gcgattaaac aatgcaacct aaagcacctt
     1021 gaggcacttt aggttgctta tgtcaccatg ctgattagca aatggttatc tatttttctt
     1081 tttatggcga ttcttacgta gacgtttttt acgcttgtgg gttgccattt tgtgtctttt
     1141 tctcttcttt ccgcttggca ttgtggtaga ttttgattgt tagtactaaa ctatttcttt
     1201 acatggttct taaccttgtt tacaaacgac ttggaaggtt tgaaagcagg aatgaaatgt
     1261 tcaggaataa tgatggtggt gttcttggaa atgttacggg cggttttttt agcacgcttt
     1321 ttcacaataa agctgccaaa gccacgcaga taaacatttt tttcctttac caaggaatcc
     1381 ttaacggctt ccatgaaagc ctcaacggtt ttctgaactg ctactttctc gattccggtg
     1441 cttttggaaa tttcgtttac gatatcagcc tttgtcattt ttgaaaatct ttagttatca
     1501 ataaattaga caataatttg caaatttgga ttgcaaatat acaagttttg tatcgattaa
     1561 taaaactgta aaggttaatt ttgacaagat tttttttctt tttatttgat agtatggatt
     1621 ttagctcaat actcttcaag tggtatagta ctaaagcacg cgatctgcct tggcgaaaca
     1681 ccattaatcc ataccatata tgggtatcgg aagtgatact acagcagaca cgagtggtac
     1741 agggcatttc gtactactat cgatttattg agcgtttccc tagcattcaa tcgcttgccg
     1801 atgccgatat agatgatgta atgaaggttt ggcaaggtct tggctattac actagagccc
     1861 gaaaccttaa agcaggtgcc attcaggtta tggaacagta caatgggaat ttaccaaaaa
     1921 gctataagga acttcagcga attaaagggt taggggctta ctcggcggct gccattgcct
     1981 cgtttgcctt tggtgaggct gtcgcggcag tcgacggaaa cgtttaccgg atcctatccc
     2041 gaatttttgg aatattcact cccatagatt ctagcactgg taaaaaggat tttttcgctt
     2101 tagccaatga actaattcct aaaaatgagc caggccgttt taaccaagct ttaattgatt
     2161 ttggagcgct acaatgtaca cctaaatcac ccggatgtac cgattgccct tttgccgatt
     2221 actgttatgc atataagaac aatctgattg tggcacttcc tgtcaagagt aaaaagaagc
     2281 cgcctgtgga acgattcttt tactacttta tgattaagca taaaggccat acctttatta
     2341 aaaggcgcca ggaaaaagat atttggcact cgctttatga gttccccatg gttgaaactg
     2401 gtgccaaatt gggccatgac caattgcttg agcgtatgga cagccttaac tttttatcgg
     2461 gttccacggt gaatgtgctg agtatctctc aaccaattcg ccatttattg agccatcgaa
     2521 ctatttgggc aacattcata atccttgagg ttgataatcc taattacatt ctcaccgaag
     2581 agtatgttag gattccgatt ggtggtgtaa gcgattactc gcttcctagg ctcattgata
     2641 tatacatggc tgccgagcct gtaatcaaat atttttcatc caaaaaataa ttgtttttga
     2701 actaattttt acttaaattg tgtaattgta aatttaaact aaacttacag gtatgtcagt
     2761 aaacaaagtt attctggtag gaaacgttgg caaggacccc gaagttcgtc accttgaagg
     2821 tggagcaact gttgcccgtt tctctttggc aaccaatg
//
LOCUS       DAOOWJ010000191         2762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_36601_length_2762_cov_4.234577, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000191 DAOOWJ010000000
VERSION     DAOOWJ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36601_length_2762_cov_4.234577"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2550)
                     /locus_tag="PLW22_11775"
     CDS             complement(<1..2550)
                     /locus_tag="PLW22_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU75423.1"
                     /translation="MKKILALIFLSFALTANSQPKKIALQANGMKPEKVNPEKFYATS
                     QNNFRFSISIDELSWDKQQAITGETYTKLWFKNSMPDGGIGEPELPVIKKLIRIPLGA
                     SVSARVSKYTTDEIELTAKGISNPLFPVQPSARKDQDTLQLPFHLKPEAYRKSSFQIV
                     KPDVSVEILGNLRGYTIARLTVRPIDYNPSKQKIRIFNDIDVDVTVQGGITKSSPASD
                     PFYSPYFDVVYKSMLNAGGAIYDTHPDLTRYPVSMLIVSHRMFQTALQPFVEWKTQKG
                     FSVTVKYTDEIGATSDAIKTYIQSVYSSATPENPAPTFLVIVGDVDQVPASATGSQSG
                     KLTDLYYASVDGDKFPEMYYGRLSATSEAELTAIINKILYYERFQFADASYLNNSTLI
                     AGADASWNPAIAQPTIKYATATHFKPSSGWSNIYEYGVINDPNNPSASSGYTGCYDPE
                     RIAVGFINYTAHCNETNWQDPALTISAVETFTNNQQYPFVIANCCLSGNFGYSESVGE
                     AWLRKANGGAVTYIGSSPNSYWKEDMYWAVGAFPMSGDNNGYVPSFEESTTGGYDAPF
                     ISAYTTAGAIMFCGNLAVTQAELNDYTRQVNSTYYWEAYNVLGDPSLTPYFKVPETNQ
                     VTFPAAVAVGINSITVGAKANSYVSLTANGQIIGTKFFDTDNVIDIAVPVLTEPGKIV
                     LTATRPLTQPFIDTIRVVVAENAYLTLSTSEIDDSEANGNSVADYGENIKVNLLVKNV
                     GKTNATNIGAKISAGTGLISLTSADSIGIGSIDAESEKWVNAAYKFSIPSNITDKYTQ
                     VFPITFTSTEGSWTSNLRITAAAPKIVFNGYTVVDTLMGNSNSQLDRNEVVD"
BASE COUNT          734 a          547 c          657 g          824 t
ORIGIN      
        1 atcaaccact tcgttacggt caagttggga atttgaattg cccattaacg tatcaactac
       61 agtgtatccg ttaaaaacta tttttggagc agcagcggtt atccttaaat ttgatgtcca
      121 tgatccttcg gttgaggtga aggtaattgg aaaaacctgt gtgtatttgt cggtgatatt
      181 ggagggaatg ctgaatttat atgctgcatt tacccatttt tccgattcgg catctatact
      241 accaattcca attgagtcgg ctgatgtaag cgaaattagg cctgtacctg ctgaaatttt
      301 tgctccaatg tttgttgcat tggttttgcc aacatttttc accagtaggt ttaccttgat
      361 gttttcgcca taatcggcaa cagagttgcc atttgcttca gagtcatcta tttcactggt
      421 cgatagggtt aggtaggcat tttcagccac aacaacccga atggtatcaa taaagggttg
      481 ggttaatgga cgagttgctg taagtacaat ctttcctggc tccgttaaaa cgggaacagc
      541 aatatcgatt acgttgtcgg tgtcaaaaaa ttttgttcca attatttgac cgttggcagt
      601 aagcgaaacg tatgagtttg ctttggcacc aacagtaatt gagttaatcc caacagccac
      661 agcagcaggg aaggttacct gattggtttc gggtacttta aagtatgggg taagtgaggg
      721 atcgccaagt acattgtagg cttcccagta gtaggtggaa ttgacttggc gggtgtagtc
      781 gtttagttca gcttgggtaa cggcaaggtt accgcaaaac attatagctc cagcggtggt
      841 gtaggcgcta ataaatggag catcgtagcc accggttgtg gattcttcaa agctgggaac
      901 gtacccattg ttatcgccgc tcatggggaa agcaccaacc gcccagtaca tatcctcctt
      961 ccagtagcta ttgggcgatg aaccaatgta ggtaacagca ccaccgtttg ctttacggag
     1021 ccatgcctca ccaactgact cagagtaacc aaaattaccc gacaggcagc agttagctat
     1081 tacaaacggg tattgctggt tgttagtgaa tgtttctaca gcgctaatgg taagagctgg
     1141 atcttgccag ttggtttcgt tacagtgagc ggtatagttt atgaacccta cagcaattct
     1201 ttcgggatcg taacaccctg tataacccga tgatgctgag ggattattag gatcgtttat
     1261 aaccccatac tcgtaaatgt tactccatcc gcttgatggc ttgaaatggg ttgctgttgc
     1321 atactttatg gtgggttgag ctatagccgg gttccatgat gcatcggcac ctgctatcaa
     1381 tgtggaattg ttcaggtatg atgcatcggc aaactgaaac ctttcgtagt aaagaatttt
     1441 gttgataatg gctgtaagct cagcctcgct ggtggccgac aggcggccgt agtacatctc
     1501 cgggaactta tcgccatcaa cactggcgta gtaaaggtcg gttagcttac ccgattgtga
     1561 accggttgcc gatgcgggaa cctgatcgac atcacccaca attaccagaa aggttggtgc
     1621 aggattttca ggtgttgctg agctgtaaac gctttgtatg tatgttttta tggcatcgga
     1681 ggttgcgcca atctcgtcgg tatactttac tgttacacta aaaccctttt gggttttcca
     1741 ctccacaaat ggctgtaatg cggtttggaa cattcggtgc gacacaataa gcatactaac
     1801 ggggtatcgg gttaagtcgg ggtgggtatc gtaaatagcg cccccggcgt ttaacatcga
     1861 tttgtaaacc acatcgaagt agggcgaata gaaaggatcg gaagcgggag aactttttgt
     1921 gatgccccct tgaactgtca catcaacatc aatatcatta aatatcctga ttttttgttt
     1981 ggaggggttg tagtcaatag gtcgcaccgt aaggcgagca atggtgtaac ccctaaggtt
     2041 accaagaatt tcaacggaaa catcgggctt tacaatctga aacgatgatt tgcggtaagc
     2101 ctctggcttt agatggaatg gcagctgtag cgtatcctga tccttacgtg ccgatggctg
     2161 aacagggaac aatgggttac taatgccttt tgcggtgagt tcaatttcat cggtagtata
     2221 cttgctgacc ctagccgaaa cagaggcgcc tagcggaata cgtattaact ttttaataac
     2281 aggcagttca ggttcaccta taccaccatc gggcatagag tttttaaacc atagctttgt
     2341 gtaggtttct ccggtaattg cctgttgctt atcccaactg agttcatcaa tactgatact
     2401 aaagcggaag ttattctgcg aggtggcata gaatttttca gggttaacct tttcgggttt
     2461 catgccatta gcctgcaatg caattttttt aggctgcgaa tttgctgtta gggcaaatga
     2521 aagaaagatt aaagctaaaa tttttttcat cagttcaaaa ttttaaataa tcccgcaata
     2581 tagtaaaacg aatatatcaa ctcaatggtt gcaataattt ttccaaataa gggtcagtaa
     2641 tttagaccat aagtaattca gccacaatag agtcgctaat aaagtaatag ctgggtggaa
     2701 ttttaaggat gttgttttcc attaccattt tacccgacgc agtaaagggt atgatttttt
     2761 tg
//
LOCUS       DAOOWJ010000192         2754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_36753_length_2754_cov_6.977399, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000192 DAOOWJ010000000
VERSION     DAOOWJ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36753_length_2754_cov_6.977399"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLW22_11780"
     CDS             <1..477
                     /locus_tag="PLW22_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOU75424.1"
                     /translation="NPDDPAFTQNEKRFYPNIGVGAYIYSYQFYAGLSLPRMLQTSFN
                     RKFDKTLKSPLYIIGGYNYELNSDWVLMPSMLAGAMIGVPMTCDITAQALYKGQFYFG
                     AHYRIGDAFGIFFDMKLKHDISVGYAFDFSLNKLSRINTGTHELMISYSFAPKWKF"
     gene            595..762
                     /locus_tag="PLW22_11785"
     CDS             595..762
                     /locus_tag="PLW22_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU75425.1"
                     /translation="MAYKISDDCTACGTCIDECPVEAISAGDIYKIDPEICTDCGSCA
                     DVCPVEAIHPA"
     gene            complement(810..1880)
                     /locus_tag="PLW22_11790"
     CDS             complement(810..1880)
                     /locus_tag="PLW22_11790"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU75426.1"
                     /translation="MEPETQKRVLLSPLAWGLGHATRIIPVIHELSHKGYLITVATNN
                     ELGALIKNNCPYSEIINFYSPKMKLGTGRASVLNLLSFLVKLPFVTIIEHFKLKRLLK
                     GKSFTLIVSDNRYGFRSSKTKSVIITHQLRVIPPKPFGFLKYLGENVIRHLLSRFDEV
                     WVPDFNNTSRIAGMLSEPNGLTNLRYIGPISRFNGISVKHLNPQWDVMVIASGPEPQK
                     TEFINFLIFCLSELNFKTLILKGNPLKINSWETIGNLTFAGSLGNNEMAMAIINSKYV
                     ISRSGYSTIMDLVYLNKGAILIPTPGQTEQEYLAHRMNELGWFKTVNQKDLSKELLLA
                     LLDQTIPTPTHFTPNQVLAFIE"
     gene            complement(1880..2653)
                     /locus_tag="PLW22_11795"
     CDS             complement(1880..2653)
                     /locus_tag="PLW22_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU75427.1"
                     /translation="MGTFLFDSIVFGPVKSRRLGVSLGINLLPTNQKICSFDCIYCEC
                     GWTDSMATLDYPTLSEVEQSLESKLVEMMKTGELPDVITFAGNGEPTLHPEFPSIIDS
                     TITLRDKYCPSAKIAVLSNSTLIHDTHIFASLLKVDQNILKLDSVFPETIEILNQPLY
                     KIEISSLIENLKRFSGSCTIQTLFVKGSFKGKAIDNTTDAEVDAWLKAIQEIKPKDVM
                     IYTIARETPTDTLHKIPLSTLQGIARKVERLGIKTQVSG"
BASE COUNT          785 a          518 c          557 g          894 t
ORIGIN      
        1 aaccccgatg accctgcgtt tacccagaat gaaaaacgct tttatccaaa tatcggagtt
       61 ggggcttaca tttactcata ccaattctat gcagggctat cgcttcctcg aatgctccaa
      121 acatccttca accgtaaatt cgataaaacc cttaaaagcc cgttatatat cataggaggg
      181 tataattatg agcttaattc cgattgggta cttatgccct caatgctggc cggcgcaatg
      241 attggtgtgc caatgacatg cgatattaca gctcaggctc tttataaggg acaattctat
      301 tttggagctc attaccgcat tggcgatgct tttgggatct tcttcgatat gaaattgaaa
      361 cacgatatat ccgttggcta tgcattcgac ttctcgttga ataagctatc aagaatcaat
      421 acaggtaccc atgagctaat gatttcatac tcatttgctc cgaagtggaa gttttaaagt
      481 tttctaaata agggttattt gaattgattt ttttggcaag ttaaatttat gtctataaat
      541 ttgcgttgag ttcagagaaa ctaattagga tacgttaaac taaattttac agagatggca
      601 tacaaaattt ctgacgattg caccgcttgc ggtacctgta tcgatgagtg ccctgttgaa
      661 gcgatttcag ctggtgatat ttacaagatt gaccctgaaa tttgcaccga ttgtggctca
      721 tgcgctgacg tttgtcctgt tgaggcaatt caccccgcat agttggtgat aaatgaatta
      781 taaaatccgt gctatatgca cggatttttt tattcaataa aggcaagaac ttgattaggg
      841 gtaaagtgtg ttggcgttgg aatagtttga tctaggaggg ctaatagtaa ttcttttgaa
      901 aggtcttttt ggttaactgt tttaaaccaa cccagctcat tcattctgtg tgctaggtac
      961 tcctgttctg tttgtcccgg agttgggatc aaaatggctc ccttgtttag gtatactaaa
     1021 tccataatgg tgctgtaacc gcttcggctt attacatact tggagtttat aatggccatg
     1081 gccatttcgt tgttacctag acttcctgca aatgtcaaat ttccaatagt ttcccagctg
     1141 tttattttaa gaggattacc ctttagtatt agggttttaa aattaagttc acttaaacag
     1201 aatataagaa aattaatgaa ctcagttttt tgtggctcag ggcccgacgc aataaccatt
     1261 acatcccatt gagggttcaa atgttttacg gagatcccat tgaaccttga aataggacca
     1321 atatatcgta aattagttaa tccatttggc tctgagagca ttccggctat acgggatgta
     1381 ttattaaagt caggaaccca tacctcatcg aatctgctaa gtaagtgtcg aataacgttt
     1441 tcacccaaat atttaagaaa accaaacggt tttgggggta taactctaag ctggtgtgtg
     1501 attatgaccg atttagtttt ggacgatcgg aacccataac ggttgtcgga aacgatgagg
     1561 gtaaagcttt taccttttaa aagccttttt agcttgaaat gctctattat tgttacaaac
     1621 ggaagtttta cgagaaatga tagcagattt aatacacttg ccctccccgt tcccagtttc
     1681 attttgggag agtaaaagtt aattatttca gaataggggc aattattttt aattagcgca
     1741 ccaagttcat tatttgtagc aacagttatt aaataaccct tatgtgaaag ttcatgaatg
     1801 acagggataa ttcttgtagc atggcctaat ccccatgcaa gtggcgatag caatactctc
     1861 ttttgtgttt ccggttccat caacccgaca cctgagtttt tatgcctaat ctttcaactt
     1921 ttctggcaat accctgtaat gttgatagtg gaattttgtg aagggtatca gtgggtgttt
     1981 cgcgagcaat ggtgtatatc atcacatcct ttggcttaat ttcctgaatg gctttaagcc
     2041 aggcatccac ctcagcatcg gttgtattgt caatagcttt gcccttaaat gaccctttca
     2101 caaaaagcgt ttgtatagta caactaccgc taaatcgttt tagattttct attaaactgc
     2161 taatctctat tttgtacaag ggttgattca aaatttcaat ggtttcagga aaaactgagt
     2221 cgagcttcag aatgttttga tcaaccttta gcaagctcgc aaagatgtga gtatcgtgaa
     2281 ttagggttga gtttgataga acggcaattt ttgccgaggg acaatactta tcccgtaagg
     2341 taatagtaga gtcaattatt gatgggaact cgggatgtag agtaggttcc ccgttgccag
     2401 caaaggttat aacatcgggt agttcgcccg ttttcatcat ttcaaccagt tttgattcaa
     2461 gcgattgctc aacctccgat aatgtcgggt agtcaagggt agccattgag tcggtccagc
     2521 cacactcaca gtaaatacag tcgaatgagc aaattttttg gtttgtaggt aaaaggttaa
     2581 ttcccaacga aaccccaagc cttcggcttt taacaggtcc aaaaacaata ctatcgaaaa
     2641 ggaaagtacc cattttaaaa tctatattag acgttttgct acactctttt cggttggtcg
     2701 cattttggta aagtcaaagt ggtcaactaa aactaaaccg ggtttcttac caat
//
LOCUS       DAOOWJ010000193         2744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_36932_length_2744_cov_5.090740, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000193 DAOOWJ010000000
VERSION     DAOOWJ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36932_length_2744_cov_5.090740"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..694)
                     /gene="cas2"
                     /locus_tag="PLW22_11800"
     CDS             complement(353..694)
                     /gene="cas2"
                     /locus_tag="PLW22_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOU75428.1"
                     /translation="MTTLNRLNQYRVMWVLVFFDLPTETKKERKQYALFRKNLIKDGF
                     SMFQFSIYTRCCPSRENADVHIKRVKSFLPPNGYVGILCITDKQFGAMEIFLATKPIK
                     ASPEPQQLELF"
     gene            complement(754..1659)
                     /gene="cas1"
                     /locus_tag="PLW22_11805"
     CDS             complement(754..1659)
                     /gene="cas1"
                     /locus_tag="PLW22_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOU75429.1"
                     /translation="MIKRTLYFGNPAYLKAKDEQMVVEYPSGDTESKSIPIEDIGVVI
                     LDSPQITITHVLLNKLLANNAAVITTNERHMPSGLLYPLESNTLQSERFTAQLEASVP
                     LKKQLWQQTVQAKIQNQAALLKSLGIDVEPMLYWARSVRSGDPDNYEGRAAAYYWKNL
                     FSGYFNDIFTRGRYEDEPNNLLNYGYAILRAVIARSLVASGLLPTLGIHHHNRYNAFC
                     LADDIMEPYRPFVDRVVLEIVKSGIDCTELTREIKAKLLVIPAIDVVIDSKSSPLMIA
                     TQRTTSSLYDCFEGASRKILYPTFS"
     gene            complement(1698..2375)
                     /locus_tag="PLW22_11810"
     CDS             complement(1698..2375)
                     /locus_tag="PLW22_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75430.1"
                     /translation="MPNSWDIPLEHGGYYHIYNRGINGTPLFYVPDNYLHFLRLYDKY
                     ITPIADTFAWCLMGNHFHLLVRVKEENEIGFLQDFNPSRSIDSGRLKWQFTHNLPESE
                     GSGSVNGKKPVPYRMFSHLFNSYTKYFNLKNNRTGSLFEKNFHRLKITSEKYFQNLVV
                     YIHQNPVHHGFTSDYRDYPWTSYGSIISTKSTKVHRETVLEWFGSKENFIDCHCKIVD
                     LDIIIDL"
     gene            complement(2419..>2744)
                     /locus_tag="PLW22_11815"
     CDS             complement(2419..>2744)
                     /locus_tag="PLW22_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75431.1"
                     /translation="PNLTLELSMQQNEMFVLGMTPEEFSDCIVKNDFKAISNKLYRVQ
                     KLAEKNYVFRHHLETQLIDDDNSKRSKRYYLIQSLGALFNLNPVKVKIDYIGNITSWN
                     NTDRV"
BASE COUNT          741 a          556 c          577 g          870 t
ORIGIN      
        1 acttaacccg ttgtgaattg ctttcagatt gtatctttga catattggtc acaactcgtt
       61 caggtgtgtg agctctggct tgatggttgt gaattgcttt cagattgtat ctttgacata
      121 ttggtcacaa cacatcgaac acatttattg ctccgagcac tgttgtgaat tgctttcaga
      181 ttgtatcttt gacatattgg tcacaacata ggagaaatta tcgttctgac ctttaattag
      241 ttaggccaat atttcggata aaaaaaagtg accaataaag tatccacaga aagctttttc
      301 tgtggatttt ttatttctgc tatatgcctt taatggttta aatgcttccg ttctaaaaca
      361 gttccagttg ctgtggttca gggcttgcct taatgggttt tgtggcgagg aatatttcca
      421 tggccccaaa ctgcttgtcg gttatgcaaa gtatgcccac gtagccgttg gggggcaaaa
      481 aactcttcac gcgcttaata tgaacatcgg cattctcacg acttgggcag caccgggtat
      541 agattgagaa ctggaacatg gagaaaccat cctttattaa gtttttccgg aaaagagcat
      601 actgcttacg ctcctttttg gtttcggttg gtaaatcgaa aaaaactaac acccacataa
      661 ctcggtactg gtttagacgg ttaagagtgg tcatagatgt ttaggagttg aaccccgaca
      721 gggttcagaa ccctgtcggg gtttgaattg aaatcacgaa aatgttggat acaatatttt
      781 acgactagca ccctcgaagc aatcgtacag cgatgatgtt gttcgttgag tggcaatcat
      841 cagggggctg cttttactat caatcaccac atctattgcc ggaattacaa ggagttttgc
      901 ctttatctcc ctggttaact cggtacaatc gataccactt tttacaattt caagcacaac
      961 cctatccaca aacggccggt agggttccat tatgtcgtcg gcaaggcaaa aggcgttgta
     1021 gcggttgtga tggtgaattc ccagcgttgg tagcaaaccg cttgctacca ggctacgtgc
     1081 tattacagca cgtaggatgg cataaccgta gttcaacaaa ttattgggct cgtcctcata
     1141 cctgccacgt gtaaaaatat cgttgaagta gcccgaaaaa aggtttttcc aatagtaagc
     1201 agcagcacgg ccctcgtagt tatcggggtc gccactcctt acacttctag cccagtatag
     1261 cataggctca acatcaatgc caagcgattt taaaagcgct gcctgattct gtattttggc
     1321 ttgtaccgtt tgctgccaaa gctgcttttt tagcgggaca cttgcctcca gctgcgcagt
     1381 aaaacgttcg ctttgcagtg tgttcgattc cagagggtaa agcagccccg agggcatatg
     1441 ccgctcattg gttgtaataa ctgcagcatt atttgccagt agtttgttga gcaacacgtg
     1501 ggttatggtg atttgtgggg aatcaagaat aaccactcca atatcttcaa tggggattga
     1561 tttgctctca gtatcaccac ttgggtattc aactaccatt tgctcatcct ttgcctttag
     1621 gtaggcaggg tttccgaaat aaagggtgcg ttttatcata tttgtttcat tatacctctg
     1681 acagggttta aaacctgtca gaggtcaata attatgtcta ggtcaactat tttacaatga
     1741 caatcaataa aattctcctt gctgccgaac cattcaagca cggtttccct atgaaccttt
     1801 gttgactttg tggagataat actaccatac gatgtccatg ggtaatccct ataatcactg
     1861 gtaaagccgt ggtgaacggg attctgatgt atataaacta caaggttttg gaagtatttc
     1921 tcggaggtta tttttagacg atggaaattt ttctcaaata gacttcccgt gcgattattt
     1981 tttaggttga aatactttgt ataagagttg aacagatgcg agaacattct gtaaggaacg
     2041 ggtttctttc cattaacgct tccagatcct tcggattctg gaaggttatg ggtgaattgc
     2101 cactttaacc ttccagagtc gatagacctg gaagggttaa agtcctgaag gaatccaatt
     2161 tcgttctcct cttttacccg caccagcaaa tgaaaatggt tccccatcaa gcaccaggca
     2221 aaggtatcgg caattggagt gatatattta tcgtaaagcc taagaaaatg caagtagtta
     2281 tcaggtacat aaaacaatgg cgtgccgttt attccacggt tgtaaatgtg gtagtaaccg
     2341 ccgtgctcca atggtatatc ccaagaattt ggcatgaggt tttttaatta tttcttcatt
     2401 gataacactg acagggtttt aaaccctgtc agtgttattc caagaggtaa tatttccaat
     2461 ataatcaatt ttaactttta ctggattcaa attgaacaaa gcccctaaac tctgaattaa
     2521 atagtatctt ttacttcttt ttgagttgtc gtcgtctatt agctgtgttt ctaaatgatg
     2581 cctaaacacg taatttttct ctgcaagttt ctgtactcta tataatttat tgcttattgc
     2641 tttaaaatca tttttaacta tacagtcaga gaattcctca ggagtcattc caaggacaaa
     2701 catctcattc tgctgcatgc tcaactccag ggtcaggtta ggcg
//
LOCUS       DAOOWJ010000194         2721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_37337_length_2721_cov_8.403976, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000194 DAOOWJ010000000
VERSION     DAOOWJ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37337_length_2721_cov_8.403976"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1494
                     /locus_tag="PLW22_11820"
     CDS             139..1494
                     /locus_tag="PLW22_11820"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU75432.1"
                     /translation="MPSIVTALYNLVGITLLSFLIGNDVALNVNVPKAVNAGSEFDVE
                     VILKKGNLSGFARLQQDLPMGLTAQLVESSLGDFSFDEQKLRFVWLRLPEGKEMRLVY
                     RVKVDERLKGSFTLKGTFAYVDNNERKTAEFQTSTISINPSTRIDPNLIVDINEFQTI
                     IPPQAPVGFVASNVRCIRQTPIPSGAENDYLVKLLVNKGNAQKFAKIEEDVPEGFTAE
                     AIETKDAVFTFKDQKVKFLWMNLPAEPRFVVSYKLIAPDGKGNITVSLKGTFSYLVGE
                     ATRVIDIIQKDIDLTNIDPAALDGLISSTFSTEGANIISGFTPYSEGGIDIPIEYKKI
                     EDKPKRKAKPEFDMEPFMLMPEKGVYYRVQIAAGHKPIDIKRYFTRYNIKYDVRTEKH
                     EGWYKYSIGSFKEYKEARDFRILIWNSTRINDAFVTAYNNGTRITVQEALMITSQKWY
                     R"
     gene            1499..>2721
                     /locus_tag="PLW22_11825"
     CDS             1499..>2721
                     /locus_tag="PLW22_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75433.1"
                     /translation="MKKLIKLHLGRLVTVLALLVVLPNALHAQIDEEEYYKGILDTIV
                     EVQNPVYKPVISLGVGYLSFWGDVRNPISSPLYGNVAGKLNVSNLVGRKHYFKGNIFA
                     IYGDIFAHDPKLSYLMQQQPLPLDDLNNPIFVNSAFKTNIFQLGLSFEYNFGHVFGPA
                     KRFKPYLSVGFSTIFFSPKGNFLNNGSGNFYYFWSDGTMRNFPENGPDAYRARIVRFD
                     SDYETDLSGKDLYKQGSISQQTYSVPIDFGFDFYLSDRVNLRVGAAFNYCFSDKIDNY
                     DSKIAKAQGYPVKNNIKDIFSFTYFTMNFDLFSDPKTILVERVFAMLGEDYDYEIFFS
                     DADGDGVFDRFDECPDTPAGVAIDSIGCPSDTDFDGVFDYMDKEPNTPEGAIVDESGS
                     QVTADVLAAMFNRKS"
BASE COUNT          797 a          555 c          578 g          791 t
ORIGIN      
        1 tttttgttag taacaattac tcgtttgaaa gcccaaaaga ttagattttt gggctttttt
       61 gttacatttt aaacaattat ttcttaatat tgtaactaat taaccctatt gtgaacctaa
      121 accttagccc aataaacgat gcctagtatc gttacagcgt tatataacct ggttggcatt
      181 accctactga gtttcctgat tggaaacgat gttgcattaa atgtaaatgt tccgaaggca
      241 gtaaatgccg gtagcgagtt tgacgttgaa gttatcttga aaaagggaaa ccttagcggt
      301 tttgctcgcc tacaacagga tttacccatg ggtttaactg cccagctagt ggaatcgtcg
      361 cttggcgact tttcatttga cgaacaaaaa ctccgattcg tatggcttcg tcttcctgag
      421 ggtaaggaga tgcgtttggt ttaccgggta aaggttgacg agaggcttaa agggtcgttc
      481 acgctaaaag ggacctttgc ctacgttgac aataatgaac gaaaaacagc cgaattccaa
      541 acctccacca ttagtattaa cccatcgacc aggattgacc ccaatttaat tgttgacatt
      601 aacgagtttc aaaccattat accaccacaa gcgccagttg gatttgtggc tagcaatgtt
      661 agatgcataa ggcaaacccc tatcccttca ggtgctgaga atgactatct ggtaaaactg
      721 ctagtaaaca agggtaatgc ccaaaagttt gccaaaattg aagaagacgt tcctgaagga
      781 tttacagccg aagcgattga aaccaaggat gctgttttca cctttaagga ccaaaaggtt
      841 aaattccttt ggatgaacct cccggccgaa ccccgatttg ttgtatcgta caaactcatt
      901 gcccccgatg ggaaaggaaa tattaccgtt agcttaaagg gaaccttctc ataccttgtg
      961 ggagaggcta ctagggttat agatatcatt caaaaggata tcgacctaac gaacatcgac
     1021 cctgctgcgc ttgacggact aatttcttca actttctcaa ccgaaggagc aaatatcatc
     1081 tcaggtttta ccccttactc cgaaggtgga attgacattc caattgagta taaaaaaatt
     1141 gaagacaagc caaaacgtaa agccaagccg gaatttgata tggaaccctt tatgcttatg
     1201 cccgaaaaag gggtttacta tagggttcaa attgcagccg gacataaacc cattgatatt
     1261 aaacgatact ttacccgcta taacatcaaa tatgatgtta gaaccgaaaa acatgaggga
     1321 tggtataagt attccatagg ttcgttcaag gagtacaagg aggctcgcga ttttagaatt
     1381 cttatctgga attccacaag aattaacgat gcctttgtta ctgcttacaa caacgggact
     1441 cgaattactg tgcaggaagc gttaatgatt actagtcaaa agtggtacag gtagttttat
     1501 gaaaaaacta attaaattac acttagggag gttagtaact gttttggcac ttttggttgt
     1561 gctaccaaat gcgcttcatg cccaaattga tgaggaagaa tactataagg gaatactcga
     1621 taccatagtt gaagttcaaa atccggttta caaaccagta atttcgttgg gtgttggtta
     1681 cctgagtttc tggggtgatg tacgaaatcc tatcagttct cccctttacg ggaatgttgc
     1741 aggaaagctt aatgtttcca acctggtggg ccgcaagcac tactttaagg gaaatatctt
     1801 tgccatatat ggcgatattt ttgcccacga ccccaagctc tcctacctta tgcaacagca
     1861 gcctttgccc ctcgacgatt tgaataatcc tatttttgtt aactcggcct ttaaaaccaa
     1921 catcttccag ttaggtttat cgtttgagta caactttggc catgtttttg gccctgcaaa
     1981 acgctttaaa ccctacctat cagtaggttt ttcaaccatt ttctttagcc caaagggcaa
     2041 tttcctcaac aacggatcgg gtaactttta ctatttctgg tccgatggaa ctatgcgaaa
     2101 tttccctgag aatgggcctg atgcctaccg tgcaagaata gtaagatttg acagcgatta
     2161 tgaaacagac ttgtcaggaa aagatctcta taagcagggt agtatctcgc aacaaactta
     2221 ctcagttcca attgattttg ggttcgactt ctatctctcc gatagggtaa acctaagggt
     2281 gggagctgct tttaattact gctttagcga taaaattgac aactacgaca gtaaaatagc
     2341 caaagcccag ggatatcctg ttaagaataa tattaaggat atatttagct ttacctactt
     2401 taccatgaat ttcgacctgt tctccgatcc aaaaacaatt cttgtggaga gggtttttgc
     2461 tatgctgggt gaggattacg attacgagat tttctttagc gatgcagatg gagatggggt
     2521 tttcgacagg tttgacgagt gccccgacac cccggctggt gttgctatcg attcaatagg
     2581 ttgcccatcc gatacggact ttgatggtgt attcgactac atggacaagg agcctaatac
     2641 tccggagggc gctattgttg acgaaagtgg ttcgcaggta acagccgatg ttctggctgc
     2701 catgtttaac cgaaagagcg a
//
LOCUS       DAOOWJ010000195         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_37871_length_2693_cov_5.242229, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000195 DAOOWJ010000000
VERSION     DAOOWJ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37871_length_2693_cov_5.242229"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..>1094
                     /locus_tag="PLW22_11830"
     CDS             134..>1094
                     /locus_tag="PLW22_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOU75434.1"
                     /translation="MQRILLVVLLFVSSTLFAQIKRSANITSNELLYHVSFLASDSLK
                     GRQPGTVQDKIAAKYIRDEFREYGLTLLGKKGYQFFSFKNFNPEFEKKTSLMINGERL
                     EFSRDFSAPTVNGSDSLCAEAIFVGKGDSVACYENIKVRRKWAVIYYPLGDSKYVYLN
                     TKPIEIAKNQGAAGIIIVSNDTLSKYVDVDFRSLPKLPIFILSPEASAKLIAPIGQPS
                     AIAKKIAEYRILSQPLGITVCGKVAKLYNSIETQNVVAMLRGTDPKYANEYIVIGAHY
                     DHLGMGEMGGSRNPGQLAVHNGADDNASGVVAMLEIAQKLASEQ"
     assembly_gap    1095..1194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1195..1665
                     /locus_tag="PLW22_11835"
     CDS             <1195..1665
                     /locus_tag="PLW22_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOU75435.1"
                     /translation="YSKNIPVFFIHTGLHEDYHTPNDDVALLNIEGMQQVASYTYRLA
                     FELSTMQKPLTFTKSSTRSLSSSRTPKIKVKLGIMPDVSGTSDEGLKVMGVTEGKPAE
                     IAGIQVGDLIIAINQKPIKNIYDYTEALSALEPGTKITVKVLRDKNEIEFSVEL"
     gene            1678..2508
                     /locus_tag="PLW22_11840"
     CDS             1678..2508
                     /locus_tag="PLW22_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75436.1"
                     /translation="MKKYLIFFFALLITITAAIYQRLTGPTHPKRISYTIDSIEYKSK
                     LIRSGESDRDTKITLNLPINATATLYYKKYKVDEDFIALPFMAIDQNKQEVYLPKMPA
                     AAKLEYYIEILDGAETKHFFKENPIIIRYKDPVPAWVLIPHILIMFLAMLFSNLTGLQ
                     AAFNMPSFKRNITISLALLFFGGLILGPIVQKFAFGAYWTGFPFGYDLTDNKTLIAFA
                     TWLIAYLINRKNDRPAIAMFAAIVTIIVFSIPHSVWGSELDYESGKVVTGFINSLILF
                     "
BASE COUNT          783 a          559 c          482 g          769 t
ORIGIN      
        1 ttccgctctg atattctgat ttttcccatt gcttaaaaaa ttggtaccaa aattgacagc
       61 aataataaaa aaattagttg atgatttaag ctatatttga attctattgt taacaataca
      121 aatttttacc atcatgcaaa ggattttact tgttgttttg ctattcgttt cctctaccct
      181 attcgcccag attaaacgaa gtgctaatat caccagtaac gaacttttat accacgtaag
      241 ctttctggct tccgatagtt taaaggggcg gcaaccaggt acagttcagg acaaaattgc
      301 agctaagtat atccgcgacg agtttcgtga atacggccta acccttttag gcaaaaaagg
      361 atatcaattc ttttccttta agaattttaa ccctgaattc gaaaagaaaa ccagcttaat
      421 gataaatggt gagaggcttg agttcagtcg cgatttttca gcacctactg taaacggatc
      481 ggactcactc tgcgccgaag caatatttgt tggcaaaggc gatagtgttg catgctatga
      541 aaacatcaag gttcgaagga aatgggctgt aatttattac ccattaggtg actcaaaata
      601 cgtttacttg aacactaaac ctattgaaat tgctaaaaat caaggtgctg ctgggattat
      661 tattgtaagt aacgacacat tatcaaagta tgttgatgtt gatttccgtt cccttcctaa
      721 actccctatt ttcattcttt cgcctgaggc ttcagcaaaa cttattgcac ctattggcca
      781 accctccgct attgctaaga aaattgctga gtataggatc ctctcgcaac ccttaggaat
      841 tactgtatgt ggaaaggtag caaagcttta taactccatt gaaacccaga atgtggttgc
      901 catgctccgc ggcaccgacc ccaagtacgc taatgaatac attgtaattg gtgcacatta
      961 cgaccattta ggaatgggtg aaatgggggg tagcagaaac cccggccaac ttgccgttca
     1021 taatggagcc gacgataacg cttcaggcgt tgtggccatg cttgaaattg ctcaaaagtt
     1081 agcatcggaa cagannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntactcc
     1201 aaaaatatcc ccgttttctt tattcataca ggactacacg aagactacca cacccctaac
     1261 gatgatgttg ccctgctaaa cattgaaggt atgcaacagg ttgcaagtta cacgtaccga
     1321 ttagcctttg agctttcaac aatgcagaaa ccgcttacct ttactaagtc aagcacccgt
     1381 tcactttcat catcacgcac cccaaaaatt aaggtcaaat taggtattat gcccgatgta
     1441 agcggtacat cggacgaagg cctaaaagtt atgggggtta ccgaaggtaa acccgccgaa
     1501 attgcaggta tccaggtagg cgatttgatt atcgccataa accagaaacc cataaaaaat
     1561 atttacgatt acaccgaagc gctttcagcc cttgaacctg gaacaaaaat aactgtcaag
     1621 gttttaagag ataaaaacga aatagagttt tctgttgaac tttaaactta tatggaaatg
     1681 aagaaatatt taattttctt ttttgcactt ctgataacaa taaccgctgc tatctatcag
     1741 cgtcttacag gtccaactca ccccaaacgg attagttaca caatcgatag tattgagtat
     1801 aaatcgaaac ttatccgcag tggggagagc gatagggata ccaaaataac ccttaaccta
     1861 cctattaatg ccacggcaac cctatattac aagaaatata aggtagatga agattttatt
     1921 gctttaccct tcatggcgat tgaccaaaac aaacaagaag tttacctacc caaaatgcct
     1981 gccgctgcca aacttgaata ctatatcgaa atacttgatg gcgctgaaac caagcatttt
     2041 ttcaaagaaa atcccatcat cattaggtat aaagatcctg tgccagcctg ggtcttaatc
     2101 cctcacattt taataatgtt cctggctatg cttttctcaa acctaaccgg tttgcaggct
     2161 gcattcaata tgccctcgtt taaacggaat ataaccataa gcctcgccct gctatttttt
     2221 ggtggtttaa tccttggccc catagtccag aagtttgcat ttggggccta ctggactggc
     2281 ttcccctttg gttatgacct taccgataat aaaaccctta ttgcttttgc aacatggctt
     2341 atagcttatt taattaaccg caagaacgat aggcctgcta tagcgatgtt tgctgcaatt
     2401 gtaaccatta tagttttctc aattcctcac agcgtatggg gatcggaact tgattatgaa
     2461 agtggaaagg ttgttacagg attcattaac tcattaatcc tcttctaatc aagttagaac
     2521 atagaaaaag aaaggccgtc ggttattcga cggccttttt gcttaactat cttctgatta
     2581 gtgtttatgt tcacacggtt tatcacaggg ttctttttgc tccttaccgc tgcagcatcc
     2641 ttctttctta atgctatcgg caggtgagtt gacctttgtt gaatcgcagc aac
//
LOCUS       DAOOWJ010000196         2691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_37901_length_2691_cov_4.932473, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000196 DAOOWJ010000000
VERSION     DAOOWJ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37901_length_2691_cov_4.932473"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLW22_11845"
     CDS             <1..448
                     /locus_tag="PLW22_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75437.1"
                     /translation="KDTLRVKQKEKPQIADTLLYHFAGGVISVKVYPWVDSKQLIEVY
                     SLKGGVVITLENINLSYNVSNTLHFRSDGSLERVNEHFNPGASMYWYDTVIHFEPDNE
                     PTWKITYQYPQRTVEDSMGEKYLWDRNQKRWVKQEIVIETIQPSAP"
     gene            complement(510..>2691)
                     /locus_tag="PLW22_11850"
     CDS             complement(510..>2691)
                     /locus_tag="PLW22_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU75438.1"
                     /translation="IIEGIDAINICTGLVPDDQLLIKGNEVFGRNCYGAGDAIRIGEG
                     TSAVLRGKQVAYEILQDAGIRCSYDTYLNISKEYIDSQQHPVRIIEEPFRPSEERRNR
                     KPFVQIDCLYGFACNPCTFACPHGAITKNSTSTVPHIDFEKCVGCMDCVYQCPGLAIF
                     GYSYAKDWLFLPIEYEAQEGAEVYLVDNNGNILGDGVIEKILRKPNKTNVARVKSLNL
                     HGDDMLAARGFIVKSNYPEPIQIKPYEYKPQEKIYVCHCDDVSLDEILETIGDRKFIS
                     ADEIKHTTRLGMGACRGKRCIKRLKTTLAPMGIQIVGDATPRGPLSNQVAMGELYPKD
                     VPETYITGINGKKARIEKVSALIAGGGIGGSALFRYMSEAGLRPVMLNYGRGASWRNI
                     AGGRTAFSLPEIADIARQNHEIFKELHKVSDIDYRPINYVTFAHDDNMLKALEASMAW
                     SDAYMVQPSDFRKEISPFMNDMLSTYKAALITRGCWQATPGKVVDLLRKIGIEHGGTV
                     KEDTELLSVHRNGNGFVALVRDHNKEYIEYHADNFVNALGPEGEKFSRQLGIFTGIYP
                     VKHQAFITRRLPWLGINGNPLGMLIDRRNYKGFVAVYGQQLAETGQIIGCASPAVDPR
                     EADKNLKINSQDFLEIASEIFSNWIPQLSSVGFQAVWAGYYVEPRMFIDTKHGLFTGL
                     RGQGFMLGMGLAKMYVDELMGRQVPEYFKRLSLEGDGLPEKAFK"
BASE COUNT          678 a          641 c          643 g          729 t
ORIGIN      
        1 aaaggatact ttacgggtta agcaaaagga aaaacctcaa attgctgaca cattgttata
       61 ccactttgca ggaggagtca tttccgttaa ggtgtatcct tgggtggata gtaagcagct
      121 aattgaggtt tacagcctga agggtggggt ggttattacg cttgaaaaca tcaacctgag
      181 ttacaatgtg tcaaacactc ttcatttccg ttccgatgga tcgttggaga gggtaaatga
      241 gcatttcaat ccgggtgcca gcatgtactg gtacgatacc gtgatccact ttgaacccga
      301 taatgaacct acctggaaaa taacctacca atacccacag aggactgttg aagactccat
      361 gggcgaaaag tatttatggg atcgaaacca gaagcgttgg gtgaaacagg agattgtaat
      421 tgaaacaatt caaccatcag ctccttaaaa aaatccccgt tggcataagc caaacgggga
      481 gtcccaaaca tatcgatttt tactggtttc tacttgaatg ccttttcggg taagccatcg
      541 ccttcaaggc ttaagcgttt gaagtactcg ggtacctgac gacccattaa ctcatcaacg
      601 tacatcttgg ccaaccccat acccagcata aagccctgtc ctcgtaagcc agtgaacaga
      661 ccgtgcttgg tgtcgataaa catgcggggt tccacatagt aacctgccca aacggcttgg
      721 aagccaacgg aactaagttg cggtatccaa ttggagaata tctccgaggc aatctcgagg
      781 aagtcctgcg agttaatctt gaggttttta tcggcttcgc gtggatcaac tgccggagaa
      841 gcacagccaa taatttgtcc tgtttcagca agttgctgcc cgtaaacggc cacaaagcct
      901 ttgtagttcc tcctatcgat aagcattcca agcggattac cgtttatgcc cagccatggc
      961 aaacgacggg taatgaaagc ctgatgctta accgggtaaa tgcctgtgaa tatccccagc
     1021 tgacggctaa acttttcgcc ctcggggcca agggcattca caaagttatc ggcatgatac
     1081 tcaatgtact ctttattgtg atcccgcact agggcgacaa atccgtttcc gttgcggtga
     1141 acgcttagca gttcggtatc ctccttaacc gtaccaccat gctcaatgcc tattttacgg
     1201 agaaggtcga caaccttacc cggtgtggct tgccaacacc cacgggttat gagtgctgct
     1261 ttgtaggtgc ttagcatatc gttcatgaag ggtgatattt ccttgcggaa atcggaaggc
     1321 tgaaccatgt atgcatcgct ccacgccatt gatgcctcca gtgcctttag catgttatcg
     1381 tcgtgagcaa aagttacata gttgatgggg cggtagtcga tatccgaaac cttatggagt
     1441 tccttgaaaa tttcgtggtt ttgacgggca atgtcggcaa tctctggtag cgagaaggct
     1501 gtgcgaccac cggcaatatt acgccagctt gctccacgac cgtaatttag cataacgggt
     1561 cgtaatccgg cttcggacat gtaacggaac agtgcactac cgccaattcc accgccagct
     1621 atgagcgccg ataccttttc gatccttgct tttttgccgt tgattccagt aatgtacgtt
     1681 tcaggaacat cctttgggta aagttcgccc atggctacct ggttgctaag tggaccacgg
     1741 ggagtggcat cgccaactat ttgaatgccc attggggcaa gtgtggtttt cagacgctta
     1801 atacagcgtt tgccacggca tgcccccata cccagtcgtg tggtgtgctt tatttcatcg
     1861 gccgaaataa acttgcggtc gcctatagtt tcaagtatct catcgagact tacatcgtcg
     1921 cagtggcaca cataaatttt ttcctgtggt ttgtactcgt atggttttat ttggataggc
     1981 tcagggtagt tggattttac aataaaaccg cgagctgcaa gcatatcatc gccatgcagg
     2041 ttcaatgatt taacccttgc cacattggtt ttattgggtt ttcggagaat tttttcaata
     2101 accccatcgc caaggatatt accgttatta tcaacgaggt aaacctcagc gccctcctgg
     2161 gcttcatact caatggggag gaaaagccaa tccttggcgt agctgtatcc gaaaatggct
     2221 aagcccggac attggtaaac gcagtccata catcccacgc atttttcaaa gtcgatgtgg
     2281 ggtacggtgc tggttgagtt cttggttatt gctccatgtg gacaagcaaa ggtacatggg
     2341 ttgcaggcaa agccatatag gcaatcgatt tgaacaaagg gtttgcggtt gcgtcgttcc
     2401 tccgaggggc ggaagggttc ctcaattatc cgtaccggat gctgctggct atcgatgtat
     2461 tccttgctga tattaaggta tgtgtcgtag ctgcaacgta tgccagcatc ctgtaagatt
     2521 tcgtaggcca cttgctttcc tcttaatacg gcactggttc cttcgccaat gcgaatggca
     2581 tcgccggctc cgtagcaatt acgcccgaac acctcgttac ctttaatcag gagctgatcg
     2641 tccggaacta aaccggtgca tatatttatg gcatcgatac cctcaatgat t
//
LOCUS       DAOOWJ010000197         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_38629_length_2651_cov_8.284284, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000197 DAOOWJ010000000
VERSION     DAOOWJ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38629_length_2651_cov_8.284284"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLW22_11855"
     CDS             complement(<1..296)
                     /locus_tag="PLW22_11855"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOU75439.1"
                     /translation="MTEQSTPEFDDVKPTQQPEDKKGKKVGAKDFISGRILTHEMVSG
                     QMPYIIFLVVLAIIYIANNYHYNNLLRTELKLRKEVKNLRAESITTAAQYMSISR"
     gene            complement(293..1192)
                     /gene="rsmH"
                     /locus_tag="PLW22_11860"
     CDS             complement(293..1192)
                     /gene="rsmH"
                     /locus_tag="PLW22_11860"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOU75440.1"
                     /translation="MAYHVPVLLTESIEGLNISPNGVYVDLTFGGGGHSRAILEKLGR
                     KGRLIAFDQDEEALKNAIDDSRFMLIRSNFRYFRNFLRYHGIDRVNGILADLGVSSHH
                     LDSPDRGFSFRFEGALDMRMNRNARLTAQKVVNDYPVEELARILKLYGEVQGAGKMAS
                     VIAKARESQKIETADQLLAILKPLVPFKIQNKVLAQVFQAIRIEVNREMESLKAMLEN
                     SSKCLLPGGRLVVISYHSLEDRMVKNYLRYGNVEGTDSKDLMGNRPTPFEQVNKKVIT
                     PTEEECLANNRARSAKLRIGERL"
     gene            complement(1193..1657)
                     /locus_tag="PLW22_11865"
     CDS             complement(1193..1657)
                     /locus_tag="PLW22_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOU75441.1"
                     /translation="MSTFIGDYECKVDEKGRVLFPSSLRKQVKSESPDRFVVKKDIYE
                     ECLVLYPIEEWEHQTAIIRRNTNPYNKEHNLFIREFFRGTAEVTLDSNGRLLLPKRLL
                     EMVGIGRDIVMAGMNGKIEIWAKEKYESAQLGSSDFANLAERILGSTPLKDE"
BASE COUNT          668 a          675 c          565 g          743 t
ORIGIN      
        1 cggcttatcg acatgtactg ggcagcggta gttattgatt cggcacggag gtttttcacc
       61 tcctttcgta acttaagttc agttcttaag aggttattat agtggtagtt gttagcaatg
      121 taaataatgg ccagcactac caaaaaaata atgtatggca tttggcccga taccatctca
      181 tgggtgagga ttcggccact aataaagtcc tttgcgccaa cctttttccc tttcttgtcc
      241 tcgggctgct gcgttggttt tacatcgtca aattccggtg tactttgctc tgtcataacc
      301 tttccccaat tctaagtttg gcgcttctgg cgcgattgtt tgccaaacac tcctcctcgg
      361 tgggagtaat caccttttta tttacctgct caaagggggt gggcctattg cccatgaggt
      421 ccttgctatc ggtaccctca acattcccgt agcgcaggta gttctttacc atcctatcct
      481 caagcgagtg gtacgatatt accaccaatc tacctcctgg taaaaggcac ttggatgaat
      541 tctcaagcat tgcctttaag ctttccatct cgcggttaac ctcaatgcgt atagcctgaa
      601 acacctgtgc caatacctta ttctgtattt taaaaggtac taagggtttt agaatggcga
      661 gtagttggtc ggcagtttca atcttttggc tttcgcgtgc cttagcaatt accgatgcca
      721 ttttccctgc accctgtacc tcgccgtata gcttcagtat tctggccagt tcctctaccg
      781 ggtaatcgtt taccactttc tgtgcggtaa gcctggcgtt ccggttcatt cgcatatcga
      841 gcgctccttc gaatcggaac gagaagcccc tatcagggct atcgaggtga tgcgacgata
      901 ctcccaggtc ggcaaggatg ccatttaccc gatcaatgcc gtggtagcgc aggaagtttc
      961 ggaaatacct gaaattgctt cggattaaca tgaaacggct atcgtcaatg gcattcttca
     1021 gtgcctcctc atcctggtca aaggctatga gacggccttt cctgccaagt ttttccagaa
     1081 tggcgcgtga atgtccacct cccccaaaag ttaggtcaac atacacacca ttggggctga
     1141 tgttcagccc ctcaatgctc tcggtaagca gtactggaac gtggtaggcc atttactcat
     1201 cctttaaagg tgttgatccc agtattcgtt cagccagatt ggcaaaatcg gacgagccca
     1261 gctgtgccga ttcgtacttc tcctttgccc aaatctcaat tttaccgttc atcccggcca
     1321 tcacaatatc gcgaccaatt ccaaccatct ccagcaaacg ttttggcaat agcaacctac
     1381 catttgaatc gagggttacc tcggctgtac ccctgaaaaa ctccctgatg aacaggttat
     1441 gctccttgtt gtaggggttt gtattccggc gtattatcgc cgtttgatgc tcccactcct
     1501 ctatggggta tagcactagg cactcctcgt agatgtcctt ttttacaaca aacctatcgg
     1561 gcgactcaga cttcacctgc ttacgaagcg atgaggggaa gaggacgcgc cccttctcat
     1621 caaccttgca ctcgtaatcg ccgataaatg tggacattgt gttttcagtt ttcaatttgt
     1681 aaaaatatac aataaaattc cactttcaac cactttctcc cactttttta cactttgtta
     1741 ataacttttt tggttgacgg tcagaaaatt atgttgaaat ccctgtaatt gttgatgatt
     1801 ggaaagtggt agatgatttt tgatataggt tgataagttg agtaaccaat agtacctttc
     1861 aactaagtat tgttttagtg gaataaaaat tctacaccca atgtcgttta gataaaaggg
     1921 aataaaaaaa ggatgctccg acagcgctca gcatgacaat catctcatta accgaataaa
     1981 agcaggtgtc acagagttac acagtgtaac acggtgttac acagagcgtg agacgtaata
     2041 ttcacgaaca acgattcacg aacaacgatt cacgaacaac ctagatgacc cctcaatatc
     2101 tcccctgaga ggggagactt tagccctgca ataaggtctc ctaaatgctt ctcccctctc
     2161 ctgtcaggag aggggagggg gtgaggtcaa ttccacgacc aacgaacaac gcttccagcc
     2221 ccgtaggggc gaaacatttg taacccaatg attactcaca tgtattgcga tgcacgctat
     2281 ggtattccaa agagcaacac tgaaacgttg catcgcaacg ggaaagcatg gcaaagaacc
     2341 tttacacgtt ctttcttgtc ttgatacaag aaagaaccaa agaaaatcaa ggctgaaaag
     2401 cccaacccga tgggtgcccc gtgggtggaa ctgccacgcg atactattcg ccaagcctgt
     2461 ggcgtgactc atatggtatc gcttactaag cccaccgcca cgttccaccc ctgacccatt
     2521 gccgggtcag gcttttatgc cactgcctgc ttcttccgct ccattctctc tcagcgataa
     2581 ggtctgggat gaagccgttt ctcacgaaca acctagatga cccctcaata tctcccctga
     2641 gaggggagac t
//
LOCUS       DAOOWJ010000198         2647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_38705_length_2647_cov_5.736497, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000198 DAOOWJ010000000
VERSION     DAOOWJ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38705_length_2647_cov_5.736497"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLW22_11870"
     CDS             <1..269
                     /locus_tag="PLW22_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU75442.1"
                     /translation="SQLNGTISRTDGTKVDRLEVKAFDIEDDAQRAKFLSGQQQQISV
                     PFSNRLVEYDPMKRIGVGLSRLGTSHAVAVGAYVYALGMIDNKS"
     gene            521..>2647
                     /locus_tag="PLW22_11875"
     CDS             521..>2647
                     /locus_tag="PLW22_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOU75443.1"
                     /translation="MKRILSVVVLGVMTFAAFSQQELLLSGQWLFRKANSTQNWLPAT
                     VPGAVHTDLHANGLIGNPYLGCYNMQLAWIDSTDWEYRKIFDLPLSIDKKARLELVFE
                     GLDTYADVYLNGVKVLTTDNMFRQWRVPCSKYLQKTNNELLVVFHSALRIIDSLASRL
                     PYRLPGGDWAYARKAAYHFGWDWGPRLITCGIWQPVYLRVMPKVDIQNLSITTLAVNQ
                     QKAILSVDFDVVAKQNKPLKLMVSDAISGEIYLNNRIIPKGVEVVERFEVDNPKLWWP
                     NGMGEQHLNRFRVSIHAGSELVYDKVHEVGIRTIKLLSEPDSIGQSFYFLVNGLPMFA
                     KGANLIPPDFFVHGISDSVWVRQVDYAARSNFNMLRVWGGGVYVPDAFMEECSRRGIL
                     VWHDFMFACSMYPSDAPYLANVEAEAVQLVKRLRRHTSLALWCGNNEVDEGWHNWGWQ
                     SQLGEISGATDSVWNGYIQLFHHLLPEVVMRCDSTRSYWPSSPMFGWGRSESTTHGDS
                     HYWGVWWGREPFERYAQKVPRFMSEYGFQGLPSETVLRMFNDGQNLPDSAQLLCHQKH
                     PVGYQTINDYMEREGLKPNDLLEWMDYSQLVQAQGYAHAIEAHRLSSPYCMGTLYWQL
                     NDCWPVVSWSGIDYAGKWKRVQHYVQNLYKPVIAGAYLVDSVVVVKAVSDSIMPILVN
                     ISAKVADINGNELSLLKQNLILQPNVA"
BASE COUNT          681 a          559 c          696 g          711 t
ORIGIN      
        1 tcagccagct caacggaacc atttctcgaa ccgatggaac aaaggtcgat aggcttgagg
       61 ttaaggcgtt cgatattgag gatgatgcgc aacgagccaa gttcctgtcg gggcaacagc
      121 aacagataag cgttcccttt tcaaatcgtt tggttgagta cgacccaatg aaacgaattg
      181 gtgtaggttt atcgcgcctt ggcacaagcc atgctgtggc tgtgggggct tatgtatatg
      241 cccttggaat gattgataat aagagttaat gcattgtgtc atccaaagtg aagcgaatgg
      301 tctcactttt attaggttaa taagatgttt cattaccagc cttgggaggg acaggacaaa
      361 ctcttactat caacgaacaa ccatcaacaa acaatttcac ttgttatccc gagcatggcg
      421 agggagctca tgcaagggta gaaagggaaa aagggcaagg ggatacttcg gcgagctcgg
      481 catgacatag gaaaaagtta tgttgaaaat actataaaag gtgaaaagga ttctatcagt
      541 tgtagtgtta ggggtaatga cttttgcagc attttcacag caagagctat tgctcagcgg
      601 tcagtggcta tttcggaaag ccaattcaac acagaactgg ctgcccgcaa ccgttccggg
      661 tgcagttcat accgaccttc atgccaatgg cttgattggc aatccatacc ttggctgtta
      721 caacatgcaa ctggcttgga ttgacagcac cgactgggag tataggaaaa tattcgactt
      781 accgcttagc attgataaaa aagctcggct tgaactggtt tttgaggggc tcgataccta
      841 tgccgatgtt tacctgaacg gggtaaaagt acttacgacc gataacatgt tcaggcagtg
      901 gcgtgttccc tgttcaaaat acctacagaa aaccaataat gagctgctgg tggttttcca
      961 ctcagccctg aggattatcg atagcttggc aagcaggtta ccctaccgtt tgcccggtgg
     1021 cgattgggca tatgcccgta aagcggctta tcactttgga tgggactggg ggccacgcct
     1081 tataacctgc ggcatatggc aacccgtcta tcttagagtg atgcctaagg ttgatatcca
     1141 aaatctttcc attactacgc ttgcggtaaa tcagcaaaaa gcgattttat ctgttgattt
     1201 tgatgtggtt gccaaacaga acaagccact gaaattaatg gtcagcgatg ccatttccgg
     1261 ggaaatctac ctaaacaatc gtataatccc aaagggcgtt gaggtggtgg aaagatttga
     1321 ggttgataat cctaagctat ggtggccaaa cggcatgggc gaacagcatc taaatcgatt
     1381 tagggtttcg atacatgctg gtagcgaatt ggtttacgat aaagtgcacg aggtggggat
     1441 aagaaccata aagttgttgt cggagcccga tagcattggt cagtcgtttt actttttggt
     1501 aaacggcttg ccgatgtttg ccaagggggc aaaccttatc ccacccgact tttttgtgca
     1561 tggcatttcc gattcggttt gggttaggca ggttgattac gcagcacgga gtaattttaa
     1621 catgctaagg gtttggggcg gaggtgttta cgtacccgat gcatttatgg aggagtgctc
     1681 ccgaaggggc attctggttt ggcacgattt catgtttgcc tgttccatgt atccttccga
     1741 tgccccatac ttagctaatg ttgaggctga ggctgttcaa ctggtaaagc gtttgcgcag
     1801 acacacaagc cttgcccttt ggtgtggtaa taatgaggtg gacgagggct ggcataactg
     1861 gggttggcag tcgcagcttg gggagatttc cggagctacc gattcggttt ggaatggtta
     1921 cattcagctt ttccaccatc ttttgcctga ggttgttatg cgttgcgact ccaccagaag
     1981 ctactggcca tcgtcgccca tgtttggctg gggtagatcc gaaagcacca ctcatggcga
     2041 ttcccactac tggggcgttt ggtggggaag ggaacccttt gagcgatacg cacaaaaggt
     2101 cccacgcttc atgagcgagt atggctttca gggcttaccc tcggaaactg ttttgcgcat
     2161 gtttaacgat gggcaaaacc tgcccgattc tgcgcagctg ctttgccacc agaaacatcc
     2221 ggtaggatac caaaccataa acgactatat ggaacgcgag gggcttaaac ccaacgattt
     2281 gcttgagtgg atggactaca gtcaactggt tcaggctcag ggttacgctc acgccattga
     2341 ggcacatcgg ttatcatcgc cgtactgcat gggaacgctt tactggcaac taaacgattg
     2401 ttggcccgtg gtatcgtggt cgggtatcga ctatgctggg aagtggaaac gggttcagca
     2461 ctacgtgcaa aatctctata aacctgtaat tgctggcgca tacctggttg atagtgtggt
     2521 tgttgtaaag gctgtatccg acagcataat gcctatactg gtaaacattt ccgctaaagt
     2581 tgctgatatc aatggtaatg aattaagttt acttaaacag aatttgattt tacagcctaa
     2641 cgttgct
//
LOCUS       DAOOWJ010000199         2646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_38722_length_2646_cov_6.307217, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000199 DAOOWJ010000000
VERSION     DAOOWJ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38722_length_2646_cov_6.307217"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLW22_11880"
     CDS             complement(<1..331)
                     /locus_tag="PLW22_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU75444.1"
                     /translation="MCKILLQRTTMVFAFLLLSTHLLFAQVTEQKEQYKVSCIAFYNI
                     ENLFDTIPGPNDTEFTPDGANKYTSARYWHKIDHMSDVISQIGGELFPGGPAILGVCE
                     IENRNVLE"
     gene            487..>2646
                     /locus_tag="PLW22_11885"
     CDS             487..>2646
                     /locus_tag="PLW22_11885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU75445.1"
                     /translation="MRRLLLMLMVGLFYSTTTVLAQYNVSGYVKDSQTGSALPGVTVQ
                     VVGINQMTTTDANGYYQFSGLQAGKYIFDYILSGYQTVENEVNVEGDLQLPDVTMNRG
                     LTDDAYATFNEVTVSLDDLESESKGQAVSGLLQSQNDAFSSAASFTFSAARFNIRGYE
                     PEYSLLFMNGVPVNDPESGFAGWSSWGGLNDVTRNRESRNGLTPTDWSFGGLGGATFL
                     NVRASQQRIGTRISYASSNRTYTNKLMFTHSTGLMENGVAFTLSGSRRWAEEGYVEGT
                     NYDAWAYFIGIEKKFNEKHSVAFTTFNAPIKRAMQGVSTDEAKNLARTNFYNPNWGYQ
                     NGEKRNARVRFQQEPTFILNHYWDINSTLKLTSTAGFSFGTYQTTGLNWYDAQDPRPD
                     YYRKLPSYWVDSDPAVISQLADAFRNDPSVGQLDWDYMYQVNYNSKDANDSLRSKYIV
                     ENRITDSRQLTLSSVVNWSILPELKVNGGIDASIYKGKNYKRMDDLLGGQYWLDIDQF
                     IERDFFGNDSLAQNDLDNPNRKVKVGDTFGYSYDANVNNGNLWAVANYALNRIDIYAG
                     ANYSYSEFWRTGNMRNGRFPDNSKGDSPKQVFHNYGVKTGATWKITGRHYLDANLAYL
                     TRAPFFRNSYISPRTSNFVIPGLTSERIAAVDVNYNLRTPYIKARVSAYYTKFKDQTE
                     LKSFYDDTYRTFINYTMRGIEKVHRGVEVGAEIKLSTT"
BASE COUNT          769 a          596 c          586 g          695 t
ORIGIN      
        1 cttcaagtac gtttctattt tcaatctcac aaactccaag tattgcaggt cccccaggaa
       61 ataattcccc tccaatttgc gaaattacat ctgacatgtg gtcaatctta tgccagtatc
      121 gggccgatgt gtacttgttt gctccatctg gggtaaactc agtatcattc ggaccgggta
      181 tggtgtcaaa taaattctca atattgtaaa acgctatgca gcttacctta tattgttctt
      241 tttgctcagt gacctgagca aacagcagat gggtagaaag cagcaaaaaa gcaaatacca
      301 ttgtggtcct ttgtagtaat attttacaca tatactgttg atttttagaa aaagtatgcc
      361 gacaaagata aaactaaacg aaaaaaaaga atgtaaaaaa tgaaaaatta tattaaaaag
      421 tgtcattttt gcaagtgcaa ttataggttt aaattttaga attatccact gaaactgaat
      481 atttaaatga ggcgattact tttaatgtta atggttgggt tgttttacag cacaaccact
      541 gtactagctc aatacaatgt gagcgggtac gttaaggact cccaaaccgg tagtgctttg
      601 cccggtgtta ccgtgcaagt ggtgggaata aaccaaatga caaccaccga tgctaacggc
      661 tactatcagt tttcgggcct tcaggcaggt aagtatattt ttgattacat cctatccggg
      721 taccaaacgg ttgaaaatga ggtaaatgtt gaaggcgatc ttcaactgcc cgatgttaca
      781 atgaatagag gtttaaccga cgatgcttac gcaacgttta atgaggtgac agtttcgctc
      841 gatgacctcg agagcgaatc gaaagggcaa gccgtgagcg ggttacttca atcgcaaaac
      901 gatgcgttct cctcagctgc ttcgtttact tttagcgcag cccgcttcaa tattaggggg
      961 tacgaaccgg aatactcgct actgttcatg aacggtgttc ctgttaacga tcctgagagc
     1021 ggttttgccg gatggagttc gtggggtggt ttaaacgatg tgacccgaaa cagggaatcg
     1081 agaaatgggt taaccccaac cgattggtcg ttcggcgggc ttggcggtgc aaccttcctg
     1141 aatgtacggg catcgcagca acgtattggg acacgtattt cttacgcatc gagcaataga
     1201 acctacacca acaagctaat gtttacccat tcaaccgggt taatggagaa tggggttgct
     1261 ttcaccctaa gcggttcgcg ccgttgggct gaagagggtt atgttgaagg taccaactat
     1321 gatgcatggg cttactttat tggcattgaa aagaaattta acgaaaagca ctccgttgcg
     1381 tttactacct ttaatgcacc aataaaaagg gccatgcagg gtgtatcgac cgatgaggct
     1441 aaaaaccttg ccagaaccaa cttttacaat cccaactggg gttaccagaa cggcgaaaaa
     1501 cgcaacgctc gtgttaggtt ccagcaagaa cctaccttta ttcttaacca ctactgggat
     1561 attaactcaa ccctgaagct aacttctact gctggattct cgtttggcac ataccaaaca
     1621 accggcctta actggtacga tgctcaggat ccccgccccg attactaccg taaactcccc
     1681 agctactggg ttgattccga ccctgcagtt ataagccagt tagccgatgc tttcaggaac
     1741 gacccatcgg ttggacagct tgattgggat tacatgtacc aggtaaacta caacagcaag
     1801 gatgccaacg atagccttcg ctcaaagtac attgttgaaa acagaattac cgactcacgc
     1861 cagcttactt tatcgagcgt ggtgaactgg agcatccttc cggaacttaa ggttaatggc
     1921 ggaatcgatg cgtcaatcta taagggaaag aactataaac gaatggacga tttgctgggt
     1981 ggccagtact ggttagacat tgaccagttt attgagcgcg atttttttgg taacgattcc
     2041 cttgcgcaga acgatttaga taacccaaat cgtaaggtta aggttggcga tacctttggt
     2101 tacagctacg acgccaacgt aaacaatgga aacctatggg cagtggcaaa ctacgcccta
     2161 aaccgtatcg atatttatgc cggagcaaac tacagctata gcgagttctg gcgtactggg
     2221 aacatgcgga atggccgctt ccccgataac tccaagggcg actcgccaaa gcaagtgttt
     2281 cataactatg gggtaaaaac cggtgctacc tggaaaatta ccggtcgcca ctacctcgat
     2341 gctaaccttg cctaccttac ccgggctccg tttttccgca attcatacat ctcgccacgt
     2401 accagtaact ttgtcattcc aggcttaacc agcgaacgaa ttgccgctgt tgatgtaaat
     2461 tacaaccttc gtacccccta catcaaagct agggtatcgg catactacac aaagttcaag
     2521 gatcaaaccg aacttaaatc gttttacgac gatacctatc gcacctttat aaactacacc
     2581 atgcgtggga tagaaaaagt acacaggggt gttgaagttg gagccgaaat taaactttcc
     2641 accaca
//
LOCUS       DAOOWJ010000200         2605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_39491_length_2605_cov_5.880784, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000200 DAOOWJ010000000
VERSION     DAOOWJ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39491_length_2605_cov_5.880784"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLW22_11890"
     CDS             <1..825
                     /locus_tag="PLW22_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOU75446.1"
                     /translation="NAQVGLTSPGEIGADVCHLNLHKTFAIPHGGGGPGVGPIAVAKH
                     LAPFLPSHPVVKTGGEKAITAVAAAPWGSASVIVISYAYILMLGAEGLTQATKMAILN
                     ANYLAARLKDHYKILYTGETGRVAHEMILDCHHFKMAYGVDTSDVGRRMMDYGYHAPT
                     VSFPVHESLMVEPTESESKQELDRFIDMMIAIKGELEDIKNGKADKADNLIANAPHTA
                     PEVTADEWKHAYSRQQAAYPLPWVMQNKFWPYVSKIDAGYGDRNLVCTCAPIEDYM"
     gene            1028..2290
                     /locus_tag="PLW22_11895"
     CDS             1028..2290
                     /locus_tag="PLW22_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOU75447.1"
                     /translation="MERVNVYLNSEIGELEGVILHTPGAEVENMTPENAQRALYSDIL
                     NLNVARKEYSQLSGVLSKVAKTYEVVDLLEKVLKNEENREYLLEKICRHENAMSLFDY
                     LLDIDAKQLARMLIEGVPLVRNTLTNFLSKERFALRPLYNFYFTRDASIAIGEDVLIA
                     KMANAVRDRESIIMEAIFSRSGIFNTQTINPTTFSNPADVTIEGGDVLVVRDDILLIG
                     NGIRTNTHGIDFILSRFLARDDKRKRHILVQELPYKPESFIHLDMVFTMLDFDKCMVY
                     EPIILQPNRYQTVHITIDKGKVKEIKTVDNLLVALKSLGIDLKPIVCGGTKDRWIQER
                     EQWHSGANFVAIGPGRVIGYGRNINTLEEMNKNGFEILRAKDIVAGKVDMNSYQKYVV
                     ALDGSELPRGGGGARCMTMPFRRKPIKW"
     gene            2347..2535
                     /locus_tag="PLW22_11900"
     CDS             2347..2535
                     /locus_tag="PLW22_11900"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOU75448.1"
                     /translation="MEKNVGKIDKVIRILIALVITGVGIYYQSWWGLLAIVPLATALT
                     GTCGLYSLFGISTCSTKK"
BASE COUNT          732 a          549 c          606 g          718 t
ORIGIN      
        1 aatgcccagg ttgggcttac cagtccaggt gagattggtg ccgacgtatg ccaccttaac
       61 ctgcataaaa cctttgctat cccacacggt ggcggtggcc ctggcgttgg cccaattgca
      121 gtagctaagc atttagctcc attcctccct tcacatccag tggttaagac cggtggcgaa
      181 aaagctatta ctgctgttgc tgctgctccc tggggaagtg ctagcgtaat tgtaatctct
      241 tacgcataca ttctaatgtt gggtgccgag ggtttaaccc aggcgacaaa gatggcaatt
      301 cttaacgcaa actacttggc tgccaggcta aaggatcact ataagattct ttacaccggc
      361 gaaaccggta gggttgccca cgagatgatt ctcgattgtc accactttaa gatggcctac
      421 ggcgtagata ccagcgatgt tggccgccgt atgatggatt acggatacca tgctccaacc
      481 gtatcgttcc cggttcatga aagccttatg gtggagccta ccgagagcga gtcaaagcag
      541 gaactcgata ggtttattga catgatgatt gccattaagg gagagcttga ggatatcaag
      601 aacggtaaag ccgataaggc cgacaacctt attgccaatg ccccgcatac agcccccgag
      661 gttaccgccg atgagtggaa gcatgcatat tcccgccaac aggctgccta cccactaccc
      721 tgggtaatgc agaataaatt ctggccctat gtaagtaaaa ttgatgcagg ttatggcgac
      781 cgtaatctgg tgtgcacctg cgcaccaatt gaggattaca tgtaaataca tttaatcgtt
      841 tagctagaaa agtgccttgg ataactccgg ggcacttttt gttttaaacc caattacttt
      901 gaaatttcct taataggcca gtcatttttt taccaataca taacgttagg tatttgtatg
      961 tttcttaaca catttagctt ttccgcgcaa acaattaact ttgcaataaa tttgatttac
     1021 aattactatg gagagggtaa atgtttatct caattccgag attggtgagc ttgagggtgt
     1081 aatactgcat acaccgggag ccgaggtgga aaatatgacc cccgaaaatg ctcaacgagc
     1141 attatacagc gatattctta acctgaacgt tgcccgtaag gagtactcgc agctcagtgg
     1201 cgtgctttca aaggtggcaa aaacctacga ggtggttgat ttgcttgaaa aagtacttaa
     1261 gaacgaggag aatagggagt atctgctcga aaagatatgt cgtcatgaga atgccatgtc
     1321 gctattcgac tatcttttag atattgatgc aaagcagctt gccaggatgc tcattgaggg
     1381 cgttccattg gttcgtaata cactcactaa ctttttaagt aaggagaggt ttgcacttcg
     1441 cccgctctat aacttctact ttacacgcga tgcatcaata gcaattggtg aggatgtgct
     1501 tatagctaaa atggccaacg ccgttaggga tagggagagt attattatgg aggcaatatt
     1561 ctcgcgttcc ggtattttta atacgcaaac cataaacccc actactttta gcaaccccgc
     1621 agatgttacc attgagggcg gcgatgttct tgtagttcgc gacgatatac tgttgattgg
     1681 caatggaata agaaccaata cccatggtat cgatttcatt ctgtccaggt ttctggctcg
     1741 cgacgataag aggaaacggc atatcctggt acaggaatta ccctataagc ccgaatcgtt
     1801 cattcacctc gatatggttt tcaccatgct cgattttgac aagtgcatgg tttatgaacc
     1861 aataattctg cagcctaacc gttaccaaac cgttcacatt acaatcgata aaggtaaggt
     1921 taaggagata aaaacggttg ataacctcct agttgctctc aagagccttg gtatcgattt
     1981 aaaaccaata gtttgtgggg gtacaaagga tcgttggata caggagcgtg aacagtggca
     2041 cagcggagca aattttgttg ccattggccc cggtagggta attggctatg gtcgaaatat
     2101 caatacgctc gaggagatga acaaaaacgg atttgaaatt ctcagagcaa aagatattgt
     2161 ggctggaaag gttgatatga actcctacca aaaatatgtt gttgctctcg atggtagtga
     2221 acttccccgc ggtggaggag gtgcgcgatg tatgactatg cctttccgtc gcaaaccaat
     2281 aaagtggtag taaacatttt ccactgtgat ttgtttctag aaatgcaatc attaaactat
     2341 gaaacaatgg aaaaaaatgt aggcaaaatt gataaggtaa tcagaattct gattgcttta
     2401 gttataactg gggtaggaat ctactaccaa agctggtggg gattactggc aatagtacct
     2461 ctggcaacgg cactaacagg aacatgtgga ctttactccc tattcgggat aagtacctgt
     2521 tccaccaaga agtaaataaa aacggggctt tgccccgttt ttttttttag ttaaccattt
     2581 gctttaccag gtttgctgcc tcctg
//
LOCUS       DAOOWJ010000201         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_42045_length_2486_cov_7.774990, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000201 DAOOWJ010000000
VERSION     DAOOWJ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42045_length_2486_cov_7.774990"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /gene="nifJ"
                     /locus_tag="PLW22_11905"
     CDS             <1..1995
                     /gene="nifJ"
                     /locus_tag="PLW22_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU75449.1"
                     /translation="GTFLLNTIWSIEEVKEKLPNSVKHYLAQNNINFYIINATQIAEE
                     IGLGNRTNTIMQSAFFKIANVIPYELAVKEMKKAIEKSYGRKGEEVLKMNYAAVDRGG
                     DVIKIDVPAEWANLEPEKQQEIPGAPEFINKVVLPINLQKGDDLPVSAFINREDGTFP
                     AGTTKYEKRGIAVNVPEWIPENCIQCNQCSYVCPHAAIRPFILTDEELKNAPEGTKTV
                     RGNGGIKDYNFRIQVSVLDCTGCGNCADVCPAKTKALVMKPIETQMAEAERFDYMHEK
                     VGYKDVLGKDKSVKNSQFAQPLFEFSGACAGCGETPYIKAITQLYGDRMIVANATGCS
                     SIYGGSAPSTPYTANKDGKGVAWANSLFEDNAEYGFGIATGVAKMRERIALKLQDALN
                     GNFTSETKEAMKDWLAGKDNADLSRAASAKLIAALEKENNPVAKELLDLKQYFEKKSV
                     WIFGGDGWAYDIGYGGLDHVLSTGEDVNILVLDTEVYSNTGGQASKSTPAAAVAKFAA
                     SGKRVRKKDLGMMAMSYGYVYVAQVAMGADHNQYFKAIKEAEAYPGPSLIIAYSPCIN
                     HGIRKGMGSTQNEAKMAVKAGYWHLYRYNPMLEAEGKNPFQLDSKEPEWNLFQDFLKG
                     EVRYTSLQLSFPQQAEELFKAAEENAKWRYNTYKKMAGNE"
     gene            2124..>2486
                     /locus_tag="PLW22_11910"
     CDS             2124..>2486
                     /locus_tag="PLW22_11910"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOU75450.1"
                     /translation="MLKPVIILFLFIPLLGFSQYNPIYKNFTVNDGLAGNETYHVFQD
                     SKGYIWVATSTGVSKFNGYSFQNFDVQSGLVDNIVFEIYEDYKGRIWFIPYSGALSYY
                     EDGKIKPYPYNNKIRQNMP"
BASE COUNT          724 a          591 c          553 g          618 t
ORIGIN      
        1 ggtaccttcc tgctcaacac catttggagc attgaggagg ttaaggaaaa attacccaac
       61 tcggtaaaac actaccttgc ccagaacaat atcaacttct acataataaa cgcaacgcaa
      121 attgctgagg aaataggttt gggcaaccgc accaatacca tcatgcaaag cgcattcttc
      181 aaaattgcca atgttattcc ctacgagttg gcagttaagg agatgaagaa ggccattgag
      241 aaatcctatg gccgcaaggg cgaagaggtg ctcaagatga actacgctgc cgttgatcgt
      301 ggtggtgatg tgattaagat tgatgttccg gccgaatggg caaaccttga acccgaaaag
      361 cagcaggaga ttcctggtgc accggagttc atcaataagg ttgtactacc cattaacctc
      421 caaaaaggcg acgatttgcc cgtaagcgca ttcatcaacc gtgaggatgg cacattccct
      481 gccggtacca ccaaatacga aaaacgtggc attgcggtga atgttcccga atggattcct
      541 gaaaactgta tccagtgtaa tcagtgctca tatgtatgtc ctcacgcagc cattcgtccc
      601 ttcatactaa ccgacgagga actcaagaat gctcccgaag gcaccaaaac cgttcgtggt
      661 aatggcggta tcaaggatta caatttccgc attcaggtaa gcgtactcga ctgtaccggt
      721 tgcggcaact gtgccgatgt ttgccctgcc aagaccaaag cccttgtgat gaaacccatc
      781 gaaacccaaa tggccgaagc tgaacgcttc gattacatgc acgaaaaggt tggttacaag
      841 gatgtgcttg gaaaagacaa atcggttaag aatagccagt ttgctcaacc actcttcgag
      901 ttcagcggtg cttgcgccgg ctgcggcgaa accccataca tcaaggctat cacccaactc
      961 tacggcgata gaatgattgt tgctaacgct accggctgct cgtcgattta cggcggttca
     1021 gccccttcaa ccccttacac agccaataag gatggcaaag gcgttgcctg ggctaactca
     1081 ctattcgaag ataatgccga gtacggcttt ggtatagcta ccggtgtggc taaaatgcga
     1141 gaacgcattg ctctgaaact tcaggacgct cttaacggca actttacaag cgaaaccaag
     1201 gaagctatga aggactggct tgccggtaaa gataacgctg atctatcgcg tgctgcatcg
     1261 gcaaaactca ttgctgctct tgaaaaagag aacaaccctg ttgccaagga actgctcgat
     1321 ctcaagcaat actttgaaaa gaaatcggtt tggatctttg gtggtgatgg ttgggcttac
     1381 gacatcggtt acggcgggct tgaccatgta ctttcaaccg gtgaggatgt aaatatactg
     1441 gtacttgata ccgaggttta ctccaatact ggtggacagg catcaaaatc aacccctgct
     1501 gcagctgtgg ctaaatttgc cgcatcgggt aagcgcgttc gtaagaaaga ccttggcatg
     1561 atggcaatgt cgtacggata cgtttatgtg gctcaggtag ccatgggtgc cgaccataat
     1621 cagtacttca aagccattaa ggaagccgag gcttaccccg gaccttcgct catcattgct
     1681 tactcaccct gtatcaacca cggtatccgt aaaggtatgg gctctaccca gaacgaagcc
     1741 aaaatggccg ttaaagccgg ttactggcac ctgtaccgtt acaaccccat gcttgaggcc
     1801 gaaggaaaga accccttcca gctcgactcc aaggagcccg aatggaacct gttccaggat
     1861 ttccttaaag gtgaggttcg ctacacctcg ttgcagctct ccttcccaca gcaagccgaa
     1921 gaactcttca aggctgccga ggagaatgcc aagtggcgct acaacaccta caaaaaaatg
     1981 gcaggtaacg agtaaactca ccatatgtaa ataaaaagcc gctcaaatgc ggcttttttt
     2041 tcttttataa actatccact ttatcaaaaa aatattactt tagcattaag taagttccat
     2101 tcattagtcc taaactttgt ttaatgttaa agcccgttat catattattt ctttttattc
     2161 ctttacttgg ttttagccag tataacccaa tttataaaaa ttttacagtt aatgatgggc
     2221 ttgccggaaa tgaaacttac cacgtattcc aagattcaaa aggatacatc tgggttgcca
     2281 ccagcaccgg cgtaagcaag ttcaacgggt atagctttca aaattttgat gttcaatcgg
     2341 gtttagtcga taatattgtt tttgaaatat atgaggatta taaaggccgc atttggttta
     2401 ttccctactc cggtgcacta agttactatg aagatggaaa aataaaaccc tacccctata
     2461 ataacaagat aaggcaaaac atgcca
//
LOCUS       DAOOWJ010000202         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_42065_length_2485_cov_7.390947, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000202 DAOOWJ010000000
VERSION     DAOOWJ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42065_length_2485_cov_7.390947"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLW22_11915"
     CDS             complement(<1..1171)
                     /locus_tag="PLW22_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75451.1"
                     /translation="MLVSLSWRNVWRNPTRSMVVVVAVALGLWGGVTAMALINGWVKQ
                     RIADSIQNEISHIQIHNPDFLLNEEVDKTFNFDSVAATLDSTKGIVGFSGRLKVFAMA
                     QTDRANSGLVVLGVRPSSERSVSGIPNNLIMGSFLEGDFKTPSIVLGKKAAEELKLVN
                     YELTDSIAQKLSAMEPRLGEQIQTLVGRRYRTRSNLINALKAELEPALFNHYSETITD
                     SCASFRLNGPVVLTFQTPYGELHSAMFRVRGIYRTSNTAFDARMAYVNFDNLALAANI
                     NPSQVHEVAILCNGNTDAVNVAKTLSATHQNLKVRSWDEISPEIALYAAFGDFMGMIY
                     VVIILFALAFGIINTMMMSVLERYREFGMLMAIGMNRLRVFSMIMMESVFLTLTGG"
     gene            complement(1206..>2485)
                     /locus_tag="PLW22_11920"
     CDS             complement(1206..>2485)
                     /locus_tag="PLW22_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU75452.1"
                     /translation="IRNAIEQYYGYIRVQHPDFADDPSLENTFEFDGSLPEKISKVPN
                     VKAVTARLETFALASSVNITKGVLVLGINPEAEKQFSNPEQFIVRYTIPKMLADSSGM
                     EPTLTDEVKRILGAHAGESFPDSAKLARIFDDQPVAAAQLNQILNLSRVLGRFITSND
                     SGVVISSRLANFLNIQVGDSLVMLSQGYQGASAAGIYPVVGIVRIPNPELDNRMVYMP
                     LQVAQSFSNAHNLLSYMAVNLYDASDEALMVTTDSVRKVLAHTGLDVKTWQDLNRVLV
                     QQIESDNQSGKAFLFLLYFVVFFGIFGTVIMMVHERMHEFGVLVAVGMQKIKLAITVL
                     LEMLIMALIGVALGYLVGLPIIHYYHVHPIRLTGEMAKMMMDMGFEPLMPMAPFDAYV
                     IWQGVSVVFMVLLVCIYPTRKILKLNTVTAIKR"
BASE COUNT          690 a          621 c          544 g          630 t
ORIGIN      
        1 ccccaccggt aagggttagg aaaaccgatt ccatcattat catgctaaaa acgcgtaaac
       61 ggttcatacc aatggccata agcataccaa actcacgata ccgctccaga acgctcatca
      121 tcatggtgtt gataatgcca aatgctaggg caaacaggat tattacaacg taaatcattc
      181 ccataaaatc gccaaatgca gcatatagtg caatttcggg cgaaatttca tcccaactcc
      241 taaccttcaa gttttgatga gtagccgata gcgtttttgc tacgtttacc gcgtcggtgt
      301 taccattgca gaggatggca acctcatgaa cctggctagg attgatgttg gccgccaaag
      361 cgagattatc gaaatttacg taagccatgc gtgcatcaaa ggctgtattg gaggtgcggt
      421 aaatacctcg aaccctgaac atcgcgctat gcagctcacc atagggtgtt tgaaatgtta
      481 gaaccacagg accgttcaac ctgaacgaag cacaggagtc ggtaatggtt tcggagtagt
      541 ggttgaacag tgcgggctca agttctgctt taagagcatt aataaggttt gagcgagtcc
      601 tatacctcct ccccacaagt gtttggattt gctccccaag ccttggctcc attgcagaga
      661 gtttttgagc aatgctatcg gttagctcat agttaaccaa tttcagctcc tcggctgctt
      721 tcttcccaag aacaatcgaa ggggttttaa aatcaccttc aagaaaactg cccattatca
      781 aattgttggg aataccacta acactccgct cgctgcttgg cctaacacct aaaacaacca
      841 ggcccgagtt ggccctatcg gtttgagcca tggcaaaaac cttcaacctt ccgctaaacc
      901 caacaatccc ctttgtagaa tcgagggtag cggccaccga gtcgaaattg aaagttttat
      961 cgacctcctc gttgagtaaa aaatcgggat tgtgaatctg aatgtgcgat atctcgttct
     1021 gaatgctatc ggcaatgcgc tgcttaaccc acccgtttat aagggccatg gcagtaaccc
     1081 caccccaaag gccaagggca acagccacca ccacaaccat gctccgggtt gggttacgcc
     1141 aaacatttcg ccagctaagt gagactaaca tgtttgtaaa gttttaatgt tttcaactca
     1201 attggttacc gtttaattgc tgttacagtg ttaagcttta gtattttccg tgtaggataa
     1261 atgcatacta gcaacaccat gaataccacc gaaacaccct gccaaatgac gtatgcatcg
     1321 aacggagcca ttggcattag tggttcaaac cccatatcca tcatcatttt tgccatttca
     1381 ccggtaagcc taatggggtg aacatggtag taatggatta ttggtaaccc aacaagatac
     1441 ccaagcgcta caccaatcaa tgccataata agcatctcga gcaaaaccgt tatggccaac
     1501 ttaatttttt gcataccaac agccacaagc acaccgaact catgcatccg ttcgtgaacc
     1561 atcattatta cagttccaaa aatcccgaag aacacaacaa aatagagcag aaaaaggaaa
     1621 gccttcccgc tctggttatc gctttcaatc tgttgaacca aaaccctgtt caggtcttgc
     1681 catgttttaa catccagccc ggtatgcgcc agcacctttc ttacgctatc ggttgtgacc
     1741 attagtgctt cgtcgcttgc atcgtacagg ttaacagcca tatacgataa aaggttatgc
     1801 gcattggaga aactttgtgc cacctgcagc ggcatgtaaa ccattcggtt gtcgagctcg
     1861 gggttgggta ttcgaactat accaacaacc ggataaatcc ctgcggcaga agcaccctgg
     1921 taaccctggc tcagcattac aagggaatcg ccaacctgaa tgtttaagaa attggccagc
     1981 ctcgatgaga ttacaacacc gctatcgttg ctggtaataa atcgccccag cacccgcgac
     2041 aggttcagga tttggtttag ctgggcagca gcaaccggtt ggtcgtcgaa aattctggcc
     2101 aactttgcgg aatcgggaaa cgattcgccg gcgtgggctc ccagtatcct ttttacttca
     2161 tcagtaagag tgggttccat acctgaacta tcggccagca tcttaggaat ggtataccta
     2221 acaatgaact gttcagggtt tgagaattgt ttttcggcct cggggtttat cccaagcaca
     2281 agcacgccct tggtgatgtt gaccgacgaa gccagggcaa aggtttcgag ccttgcagtt
     2341 actgccttaa cattaggaac cttcgaaatt ttttcaggca ggctcccgtc gaactcaaag
     2401 gtgttctcca gcgagggatc gtcggcaaaa tcggggtgtt ggacacggat atagccgtag
     2461 tactgctcaa tggcattgcg tatca
//
LOCUS       DAOOWJ010000203         2457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_42728_length_2457_cov_7.680683, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000203 DAOOWJ010000000
VERSION     DAOOWJ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42728_length_2457_cov_7.680683"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLW22_11925"
     CDS             <1..207
                     /locus_tag="PLW22_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017140981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU75453.1"
                     /translation="VLFYDHLILYTPELIVPHKQLHKRRFILEPLKEIAPDFIHPLFS
                     ITINEMAISCTDAGKVWKYEPVNA"
     gene            complement(239..754)
                     /locus_tag="PLW22_11930"
     CDS             complement(239..754)
                     /locus_tag="PLW22_11930"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOU75454.1"
                     /translation="MKLKVMVIISLFFGTSCKSQDLQTVPELNLERYAGTWYEIARLP
                     NSFEKNLECITATYTIRPDGRVTVENKGVKADGSMSSIKGVAWVSDKNEPAKLKVRFF
                     WPFAGKYWVVYIDNDYRYALVGEPKRKYFWILSRTPQVDSLSYKQLVDIASQKGFDTQ
                     RLITVNQKCPE"
     gene            complement(850..1500)
                     /gene="upp"
                     /locus_tag="PLW22_11935"
     CDS             complement(850..1500)
                     /gene="upp"
                     /locus_tag="PLW22_11935"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOU75455.1"
                     /translation="MIHELGQQNSILNQFIAELRDVNVQNDFMRFRRNLERCGEIFAY
                     ELSKVLSYQTVQVQTPLGTADVSVPTDNIVLATILRAGLPLHQGMLNYFDKAQNAFIS
                     AYRKYDKDGTFRIQFEYISCPSIEGKTLVLIDPMLATGASMLLAYKALLEHGKPLHTH
                     IVAVIGSKEGVNYMKRNLSPSEATIWVGAIDDELTVKSYIVPGLGDAGDLAYGKKD"
     gene            1571..2194
                     /locus_tag="PLW22_11940"
     CDS             1571..2194
                     /locus_tag="PLW22_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75456.1"
                     /translation="MNNAIANILKHDSVIAQLAGKLEVKPLPVTNDIYLDMLENIVSQ
                     QLSGRVATTIFNRFLDLFPDRYPNPNHLLKLDDIELRKVGLSGAKSTYVKAMAEFAMN
                     HDLNVDTIQQLPDDEVIKLLTQVKGVGVWTAQMLLIFSLGRNDVFPVDDLAIRQGMVE
                     LYHLKSDGKKLRHQLLQISERWAPYRTWGTRLIWAYVNSKKQNATRV"
     gene            complement(2191..>2457)
                     /locus_tag="PLW22_11945"
     CDS             complement(2191..>2457)
                     /locus_tag="PLW22_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75457.1"
                     /translation="PPQKPTAKNKRHSGVIRLLLSESPKTKPIIKLPKMLTVKVPHGK
                     LLSVCSWKYLLTRKRNALPTKPPMPTIKIDLNMNFFLRRYKTIA"
BASE COUNT          659 a          558 c          535 g          705 t
ORIGIN      
        1 gtgctattct acgaccatct gatactatac acccctgaac tcattgttcc ccacaagcaa
       61 ctccataaac gcagattcat tcttgagccc cttaaggaga ttgcccctga ctttattcac
      121 cccctgttct ccatcaccat taatgaaatg gccatttcct gtaccgatgc cggaaaggta
      181 tggaagtacg aaccggttaa tgcctaagac acatttatcg taatagctaa aaatgcccct
      241 attcggggca tttttgattt actgtaatca gtctttgggt gtcgaatcct ttttggcttg
      301 ctatatcaac cagttgctta taggataacg aatctacttg tggcgtacgt gatagaatcc
      361 agaaatactt tcgcttgggc tcccccacaa gcgcgtagcg gtaatcgtta tcaatgtaaa
      421 ccacccagta cttgcctgca aaaggccaaa agaaccttac ttttagctta gcaggctcat
      481 tcttgtcgga aacccaggcc actcctttaa tgctgctcat gctgccatcg gcttttactc
      541 ccttgttttc aacggtaacc cgcccatcgg gccgaatggt ataggtggct gttatgcact
      601 ccaagttctt ctcaaaactg ttaggtaggc gagctatttc gtaccatgta ccggcgtacc
      661 gttcaaggtt aagttccgga acagtttgca aatcctgcga tttgcaggat gtgccaaaaa
      721 ataatgagat gattaccata accttaagtt tcatggtcga gtttattagt tagaatattg
      781 aattcagcaa tctgaatgcg gcggtgtttt attaatcaga atactaaaca ccaaaagtaa
      841 aacattcgct taatcctttt tcccgtaagc cagatcgcct gcatcaccaa ggcctggaac
      901 aatgtacgat ttcactgtga gctcatcgtc aatagcacct acccaaatgg ttgcctcgct
      961 gggcgaaagg ttacgtttca tgtagtttac cccttccttt gaaccaatta ccgccacaat
     1021 atgcgtatgt agcggcttac cgtgttcaag taaggccttg tatgccaaca gcatcgatgc
     1081 gccagtggct aacatggggt cgataagtac cagggtttta ccctcgatac tgggacagga
     1141 aatatactcg aactgaatgc ggaaggtacc atccttatcg tatttccggt aagccgaaat
     1201 aaaggcgttc tgagccttat cgaaatagtt taacattccc tgatgtaggg gtaagccggc
     1261 acgaagaatg gtggccaata caatgttgtc ggttggaact gaaacatcgg ctgttccaag
     1321 tggggtttgg acctgtacgg tttggtagct caataccttg ctaagctcgt aggcaaaaat
     1381 ttcgccgcat cgttcaagat tgcggcggaa acgcataaaa tcgttctgaa catttacatc
     1441 tcttaactct gcaataaact ggttaagaat tgagttctgc tgaccaagtt cgtgtatcat
     1501 cggaaatgtt ttatcttagc aaagttacaa aaatatcaac ctactttgca attctttttt
     1561 ttagtaagcc atgaataatg ccatagccaa catccttaag catgacagcg taatagccca
     1621 actggctggt aagcttgagg ttaaacccct gcctgttacc aacgatatct accttgatat
     1681 gctggaaaac attgtaagcc agcagctctc gggcagggtg gctaccacca ttttcaaccg
     1741 ctttctggat ttatttcctg acagataccc taacccgaat catctcctaa agctcgatga
     1801 catagaactc cgaaaggttg gcctatcagg tgcaaaatcc acttacgtta aggctatggc
     1861 tgagtttgca atgaaccacg atttaaatgt tgataccata cagcaactcc ccgatgatga
     1921 ggtaattaag ctgctaacac aggttaaggg tgttggggtg tggacagccc agatgttact
     1981 catcttctcc ctgggccgaa acgacgtttt tcccgttgat gatcttgcca ttcgccaggg
     2041 aatggttgag ctttaccatc ttaaaagcga tgggaaaaag cttcggcacc aattattgca
     2101 aatatcagag agatgggcgc cctaccgcac ctggggaacc cgcttgattt gggcatacgt
     2161 aaatagcaaa aaacaaaatg ctactcgtgt ttaggcaata gttttgtatc ttcgcaaaaa
     2221 gaaattcatg ttcaggtcaa ttttaatagt tggcataggt ggttttgtgg gaagtgcgtt
     2281 gcgtttcctt gtgagtagat acttccagct gcacacactt agtaattttc catggggaac
     2341 cttaacggtt aacattttgg gcagctttat aatcggtttg gtttttgggc tttcggaaag
     2401 gagcaaccta atcacgcctg agtggcgttt gttcttggca gtgggctttt gtggggg
//
LOCUS       DAOOWJ010000204         2431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_43295_length_2431_cov_7.258838, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000204 DAOOWJ010000000
VERSION     DAOOWJ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43295_length_2431_cov_7.258838"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PLW22_11950"
     CDS             <1..779
                     /locus_tag="PLW22_11950"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOU75458.1"
                     /translation="AVFDTSFHQTMPKYAYLYAIPHDYYDKYKIRRYGFHGTSHKYVA
                     QKACRILGVDFESQKIITCHLGNGASIAAIKHGKSVDTSMGFTPVEGLIMGTRSGDVD
                     AGILLYLAEKENLSIKEVNDVINKKSGVLGISGLSSDMRDLENAAAQGNERAQLALDM
                     YYYRVKKYVGAYAAAMDGVDLIIFTGGIGENDPILREKVSTAIDFMGIDFDSEANKGV
                     RGKDKLLTKPSSKVKVMVITTNEELVIATDTANIVKELKK"
     gene            805..1710
                     /locus_tag="PLW22_11955"
     CDS             805..1710
                     /locus_tag="PLW22_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOU75459.1"
                     /translation="MRVEKLDQLFDILRSKPRKRLVAAYANDHHTIEAVSMAIDMGIV
                     DATLVGDEATIKKVCGEHNIDFSKFRVVQEADEMKAARKAVELINKGEGDVLMKGLVS
                     TDKYMRAILDKENGLLPPKAVLSHVTVVQVPTYHKLLIVGDVAIIPAPDLNQKIAITN
                     YLIQTAHSLGIEMPKVALNAATEQMSAGMQACVDAAIISKMADRGQIKGAIVDGPLAL
                     DVAIDKESAQIKKVSGEVAGDADCILFPNIESGNVFFKACTKLAKGELGAMVMGAKVP
                     CVLTSRGDSVQSKMYSIALAANAAK"
     gene            1745..>2431
                     /gene="buk"
                     /locus_tag="PLW22_11960"
     CDS             1745..>2431
                     /gene="buk"
                     /locus_tag="PLW22_11960"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOU75460.1"
                     /translation="MEEYKILAINPGSTSTKIAVFRNNKNIFLKNIKHSADDLAKFKT
                     IADQFSFRKEIILEELKQAGIDVNKISAVVGRGGLLKPIESGVYEVNEQMKVDLRNSI
                     MGEHASNLGALIADDIARSLPSAKAYIADPVVVDEMEDVARISGHPRFKRVSIFHALN
                     QKAIARLHAKSIDKKYEELNLVIAHLGGGISVGAHRQGRVIDVNNALDGDGPFSPERA
                     GTVPAGQLAKL"
BASE COUNT          709 a          514 c          579 g          629 t
ORIGIN      
        1 tagctgtatt cgatacatcg ttccatcaaa ccatgcctaa atatgcttac ctctacgcta
       61 ttcctcatga ctactacgat aagtataaaa tccgtaggta tggctttcac ggaacaagcc
      121 ataagtatgt agcccaaaag gcatgtcgca tacttggtgt tgatttcgag agccagaaaa
      181 tcattacctg tcacctaggt aatggcgcat caatagctgc aataaagcat ggtaaatcag
      241 ttgatacctc aatgggtttt actccagtag aaggtctaat aatgggaacc cgcagcggtg
      301 atgttgatgc tggtatttta ctttaccttg ctgaaaagga gaacttaagc attaaagagg
      361 ttaatgacgt aattaacaag aagagtgggg tgcttggtat atcgggttta tcgtccgata
      421 tgagagacct ggaaaatgcc gctgctcagg gcaatgagcg tgctcagctt gcactcgata
      481 tgtactacta ccgcgttaaa aagtatgttg gagcttacgc tgctgcaatg gatggtgttg
      541 accttatcat tttcaccggt ggtataggcg agaacgatcc aattctccgc gaaaaggttt
      601 caaccgctat cgatttcatg ggtatcgatt ttgattctga agccaataaa ggtgtgcgcg
      661 gtaaggataa gttgctaacc aaacctagtt caaaggttaa agtgatggta atcaccacca
      721 acgaggagct tgtgattgcc accgatacgg caaacattgt aaaagaactt aaaaagtagt
      781 ataaacacaa aatgcgataa agccatgaga gtagaaaaac ttgatcagct attcgatatt
      841 cttcgtagca aaccccgtaa acgattagta gcagcttatg ctaacgacca tcacaccatt
      901 gaggctgtta gcatggctat tgacatgggt attgttgatg ccactctggt gggcgatgag
      961 gcaaccatca agaaagtttg cggcgaacac aatatcgatt tttcaaagtt ccgcgttgtt
     1021 caggaagccg atgagatgaa agcagcccgc aaagctgttg agcttattaa taagggtgaa
     1081 ggcgatgtac tgatgaaagg tctcgttagc accgataagt acatgcgtgc cattttggac
     1141 aaggagaatg gcttactacc tcctaaggcg gtgctaagcc atgttaccgt tgttcaggtg
     1201 cccacatacc acaaacttct tatagttggc gacgtggcaa tcattcctgc tccagatttg
     1261 aaccaaaaga tagccattac caattacctc atccaaaccg cccattcact tggcattgaa
     1321 atgccaaagg tagccctcaa tgctgcaact gagcaaatgt cggcaggtat gcaggcatgt
     1381 gttgatgcag ccattatctc aaaaatggcc gataggggtc agataaaggg tgccattgtt
     1441 gatggacctc ttgcccttga tgtggcaatc gataaggaat cggctcagat taaaaaggtt
     1501 tcaggtgagg ttgccggtga tgccgactgc atacttttcc caaatattga atcgggtaat
     1561 gtattcttta aggcctgcac aaagcttgct aagggcgaac ttggagccat ggttatgggc
     1621 gcaaaagtgc cttgcgtgct aacatcacgt ggcgacagcg tacaatcaaa aatgtattca
     1681 atcgcccttg ctgccaacgc tgcaaaatag tacaaaccag taacctgaaa atattataat
     1741 tagcatggaa gaatataaaa tactggctat caaccccggt tccacgtcaa ccaaaattgc
     1801 cgtattccgg aataataaaa acattttcct gaaaaacatc aaacactcgg ccgacgattt
     1861 ggcaaagttt aagaccattg ccgaccagtt cagtttccgt aaggaaatta ttcttgagga
     1921 gttgaaacag gctggtattg atgttaacaa aatatcggcg gtagttggtc gtggtggact
     1981 gctaaaaccc attgaatcgg gggtttacga ggttaatgag caaatgaaag ttgacttacg
     2041 caacagcata atgggcgagc atgccagtaa ccttggcgct ttaattgccg acgatattgc
     2101 ccgttcgtta ccctcggcta aggcatacat tgccgacccg gtggtggttg acgagatgga
     2161 ggatgttgct cgcatttcag gccatccccg tttcaaaagg gtatctatct tccatgctct
     2221 aaaccagaag gccattgccc gtttgcatgc aaaaagtatc gataaaaagt acgaggagct
     2281 caaccttgtt atcgcacacc ttgggggagg tatatcggtg ggtgcacatc gtcagggcag
     2341 ggtaattgat gtaaacaacg ccctcgatgg cgatggacct ttctcacctg agcgtgctgg
     2401 aactgtacct gctggtcagc ttgcaaagct t
//
LOCUS       DAOOWJ010000205         2387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_44424_length_2387_cov_5.461835, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000205 DAOOWJ010000000
VERSION     DAOOWJ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44424_length_2387_cov_5.461835"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLW22_11965"
     CDS             <1..626
                     /locus_tag="PLW22_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOU75461.1"
                     /translation="IETSLIKSGVAALANQATNWLVGNKIPERMGSEHPNIVPYGKIF
                     YTADGKPLVLAIGSDKQFAELCTVIGKPELATDERFSTNFNRVKHRMECNNAVQEYIS
                     KFNRDYLLAEFEKRAIPAGGVFNMKEVFELPEVQDLVMESKTTSGMPIKGLRTVAFSM
                     GENTKPIKLQAPPHYGEHTMSVLKEMLGYSAEEINSLIEKKVVYARK"
     gene            613..1737
                     /locus_tag="PLW22_11970"
     CDS             613..1737
                     /locus_tag="PLW22_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75462.1"
                     /translation="MHESKKNLAFIDGSRLITEALVRAGADTFVGYPITPANLLYLYA
                     SKRFPTMLAAPDEITTLQWMSGFAVAGHVPVTATSYPGYALMVESIGMAAMMELPMVI
                     VLVQRLGPATGTATCGAQGDLNVVYGTMSGGYSIPTFSISNYHDCWELSAKAVHTAIE
                     LRTPVVLLTSKEMVMTLQSFDWSNLPEIQPKKVKHYKGEGTYLPYKADETLVPDFLPV
                     SQNKHQVRITASTHDTKGILQNVLPEAIENTKRLEQKLRKHIDEYTTYELDEQQGSET
                     LIITYDISSQASREAVVHLREKGKKVSFLVAKTLLPIPQVYVDIANRYTRVVVVEENL
                     TGQLRHLLFGAAGRKGVSGVNGIAKMISPTEIVEEVMRYE"
     gene            1730..>2387
                     /locus_tag="PLW22_11975"
     CDS             1730..>2387
                     /locus_tag="PLW22_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU75463.1"
                     /translation="MSNTRFLTDAQLPFCKGCGHALVAQNTEKALQKLNVNPLDVVLV
                     TDIGCHGIVDKSFLTHTVHGLHGRSSALAAGITAGLNNPGKKVIVFTGDGGATIGMQH
                     LIGGAHLGFDMTVVVHNNMLYGMTGGQPSEFTPCGFKTPTLPEGSTKPGYDICELMVA
                     AGASYVERVIGIGDYSDSLAKAFTAPGFSLVEVMEICPSYGVKSNPGMKLSKLVEDAG
                     W"
BASE COUNT          635 a          515 c          592 g          645 t
ORIGIN      
        1 tcattgaaac aagcctgata aaatcaggtg ttgctgctct ggccaatcag gctactaact
       61 ggttggttgg taacaaaatc cctgaacgaa tgggctctga gcatcccaat attgtacctt
      121 acggtaaaat attctataca gccgatggaa aacccttagt gcttgccatt ggtagcgata
      181 agcagtttgc tgagctttgc acagtaattg gaaaacccga actggctacc gatgaacgtt
      241 tctcaaccaa tttcaatagg gttaagcacc gcatggagtg taacaatgct gtgcaagaat
      301 acatttcaaa gttcaatcgc gattacttat tggcggagtt tgagaaacgc gctattccgg
      361 ctggcggggt gttcaacatg aaggaggttt ttgaactccc tgaggtgcag gatttagtga
      421 tggagagcaa aacaacatcg ggtatgccaa taaagggatt acgaacagta gctttcagca
      481 tgggtgaaaa cactaagccc attaagcttc aggctccccc gcattacggt gagcatacca
      541 tgtctgttct taaggagatg ctgggctact cagctgagga aattaatagt ttaattgaaa
      601 aaaaggtggt atatgcacga aagtaaaaag aaccttgctt ttattgatgg ttcgagatta
      661 ataaccgaag ccttggtaag ggctggcgcc gatacctttg tgggctatcc tataacaccc
      721 gccaacttgc tttaccttta tgctagcaag cgtttcccca ccatgctggc tgcccccgat
      781 gaaattacca cgctacaatg gatgagtggt tttgcagtgg ctggtcatgt gcctgttacg
      841 gctacctctt atcccggata tgccctgatg gttgaatcaa ttggcatggc tgccatgatg
      901 gagttaccca tggtaatagt gctagttcaa cgtctaggac cagcaacagg taccgcaacc
      961 tgcggtgcac agggcgattt gaatgtggtt tatggtacta tgtcgggtgg ttactcaata
     1021 cccacttttt caattagtaa ctatcacgat tgctgggagt tatcggctaa agccgtgcat
     1081 actgccattg aacttcgtac gccggttgtg cttcttacat caaaggagat ggtgatgacc
     1141 ctacagtcgt tcgactggtc aaaccttcct gagattcagc ccaaaaaagt taagcactat
     1201 aaaggtgaag ggacttacct gccttacaag gctgatgaaa ccctggtgcc tgacttttta
     1261 ccagtaagcc aaaacaagca tcaggttcgt attacagcat cgacccacga taccaaggga
     1321 atcctacaaa atgttcttcc ggaagccatt gaaaacacca agcgccttga gcaaaagttg
     1381 cgtaagcata ttgatgaata taccacatat gagcttgatg agcagcaagg ttctgaaacc
     1441 ttaattatta cctacgacat ttcttcgcag gcttcgcgcg aggctgttgt tcaccttaga
     1501 gagaagggca agaaggtttc gtttttggta gcaaaaactc ttcttccaat accacaggtt
     1561 tatgttgata ttgctaaccg ctatacccga gtggtggtgg ttgaggaaaa ccttaccggg
     1621 caactgcgtc acctgctgtt tggcgctgcc ggccgtaagg gagttagcgg tgtaaatgga
     1681 attgctaaaa tgataagtcc aaccgaaata gttgaggagg taatgcgcta tgagtaatac
     1741 ccgtttctta accgatgccc agttaccgtt ttgcaagggt tgtgggcatg cgttggtagc
     1801 ccaaaatact gagaaggcct tacaaaaact taatgtcaac ccccttgacg tggtactcgt
     1861 taccgatatt ggatgccatg gaattgtgga taagagcttc ctaacgcata ccgttcatgg
     1921 gttgcatggt cgctcatcgg ctttggctgc cggtattact gcgggtttaa ataaccccgg
     1981 caaaaaggtg atagttttca ctggcgatgg tggtgcaacc attggcatgc agcatttgat
     2041 tggtggagct cacttgggct ttgacatgac agttgtggta cacaacaata tgctttatgg
     2101 tatgacaggt ggtcaaccat cagagtttac cccgtgcggt tttaaaacgc ctactctccc
     2161 cgagggaagc acaaaacctg gctatgacat ctgtgagtta atggttgctg ctggagcatc
     2221 gtacgttgaa cgtgttattg gtattggcga ttattccgat tcgctggcca aagcatttac
     2281 agcccctgga ttctctttgg ttgaggtcat ggagatttgc cccagctacg gtgtaaaatc
     2341 gaatccggga atgaagctta gcaagctcgt tgaggatgcc ggttgga
//
LOCUS       DAOOWJ010000206         2386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_44458_length_2386_cov_6.669241, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000206 DAOOWJ010000000
VERSION     DAOOWJ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44458_length_2386_cov_6.669241"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1561
                     /gene="guaB"
                     /locus_tag="PLW22_11980"
     CDS             80..1561
                     /gene="guaB"
                     /locus_tag="PLW22_11980"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOU75464.1"
                     /translation="MSFLDDKVVAEGLTFDDVLLVPAYSEVLPRNVDLTSRFTRNITI
                     KAPIVSAAMDTVTESALAIAIAREGGIGVIHKNMSVEAQARQVREVKRAENGMIYEPV
                     TIHPDATVGDALAMMKEYRIGGIPVVNNENILIGIVTNRDLRFQENMRTPIRDVMTSK
                     GIITTSQSIDLEKATEILKQHKIEKLPVVDENNKLIGLLTYKDITKVKDNPNASKDAK
                     GRLRVAAGVGVTADTLIRVRALYEADVDAIVIDTAHGHSKGVIEMLKMVKKEFPKLEV
                     IVGNIATADAAKMLVDAGADAVKVGIGPGSICTTRVVAGVGVPQLNAIWDVASAIQGS
                     GVPVIADGGIRYSGDIVKALAAGADTIMAGSLFAGVEESPGETIIYNGRKFKSYRGMG
                     SLDAMQQGSKDRYFQDVEDDIKKLVPEGIVARVPYKGTLAEVFYQLIGGLRAGMGYCG
                     APNLDALKKAKFVRITHAGIIESHPHDVAITREAPNYSRDTSY"
     gene            1605..>2386
                     /locus_tag="PLW22_11985"
     CDS             1605..>2386
                     /locus_tag="PLW22_11985"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU75465.1"
                     /translation="MKKVFRVAIITAGVLAGQIAYAQKQGSETLLTINNTPISADEFI
                     SLYIKNTKATGAKIDKSTLDDYLNLFINFKLKVQAARDAGIDTLSSFRNEYNGYIDQL
                     AQSYFIDQETLDALTREAYQRMKEEVSASHILISIPETVTGADTLKYYQKALEARSKV
                     INGEPFGKVALTTSNDPSVSRNNGYLGWFSAFQMVYPFETAAYSTPVDSVSMPVRTRF
                     GYHIIKVHGRRPAKGQIKVAHIMVRVNPNASPQEMENARQKIV"
BASE COUNT          677 a          550 c          568 g          591 t
ORIGIN      
        1 tgcatattgg gctgagccaa ttgcaggtat gctatttttt tgtaacttgc cgcattttta
       61 aacagtaaac cagttagcca tgtcgttctt agatgataaa gtagtagccg aaggtttaac
      121 cttcgacgat gtgctgttag ttccggcata ctcagaagta ttaccccgca atgttgattt
      181 aacatcgcga tttacgcgaa acattacaat taaagcgcca attgtttcgg ctgccatgga
      241 tacggtaacc gaatcggcac tggccattgc cattgcccgc gaagggggca taggggttat
      301 ccataaaaat atgtcggttg aggctcaggc tcgacaggta cgcgaggtaa aacgtgccga
      361 gaatggaatg atctatgaac ccgttaccat tcatcccgat gctactgttg gtgatgccct
      421 ggccatgatg aaagagtacc gtattggagg cattcccgta gttaacaatg agaacatcct
      481 tataggcatt gttaccaaca gggatctacg ctttcaggag aatatgcgta cccccatcag
      541 ggatgtaatg acctctaaag gaattattac aaccagccaa tctatcgacc ttgaaaaggc
      601 aaccgagata ctaaaacagc ataagattga aaaacttccg gtggttgacg agaataataa
      661 gctaattggt ttgctcactt ataaggatat caccaaggta aaagataatc ccaatgccag
      721 taaggatgcc aagggacgtt taagggttgc agcaggcgtt ggagttacgg ccgatacact
      781 aatccgcgta agggcactat acgaggctga tgttgatgcc attgttattg atactgcaca
      841 cggccacagc aagggtgtga ttgagatgct aaaaatggtg aaaaaggaat tccccaagct
      901 tgaagtgatt gttgggaaca tagccacagc cgatgccgct aaaatgcttg ttgatgctgg
      961 agccgatgcg gttaaggtag gcattggccc gggttcaata tgtacaaccc gagttgttgc
     1021 aggcgttggt gttcctcagc taaacgctat ttgggacgtt gcaagtgcca ttcaaggctc
     1081 aggcgttcct gtaattgccg atggtggaat tcgttactca ggcgatattg taaaagccct
     1141 agcggcaggc gccgatacca ttatggccgg atcgcttttt gcaggtgtgg aagaatcacc
     1201 aggcgaaaca atcatttaca atggccgtaa gttcaagtcg tacagaggaa tgggttcact
     1261 cgacgccatg cagcagggtt ccaaggaccg ttacttccag gatgttgagg acgacattaa
     1321 aaaacttgta cccgaaggca ttgtggctcg cgttccctac aagggaaccc tcgccgaggt
     1381 tttctaccag cttattggcg gcctacgtgc cggtatgggc tactgtggcg caccaaacct
     1441 cgatgcactc aaaaaagcca agtttgttcg gattactcat gcaggtatta ttgaaagcca
     1501 cccccacgat gtggcaatca cacgcgaggc tccaaactat agtcgcgata ccagctatta
     1561 gtccaaacta atgttttgta aggtaaccct taaacccaaa accgatgaaa aaggtatttc
     1621 gagtagcaat cattacagcc ggagtgttag caggacaaat tgcttatgcc cagaaacagg
     1681 gctctgaaac gcttttaacc atcaacaata ctcctatttc agctgatgag tttatatcgc
     1741 tttacattaa aaacaccaag gctactggtg caaaaattga taaatcgacc cttgatgact
     1801 acctaaacct tttcatcaac tttaagctaa aggttcaagc agcccgcgat gcaggaatcg
     1861 ataccctatc atcgttcagg aatgagtata atggctacat tgatcagctt gcgcaatcgt
     1921 actttatcga tcaagaaacc ctcgatgcgc ttacgcgcga ggcttaccaa cggatgaagg
     1981 aagaggttag cgccagtcac atccttatta gtattccgga aacagtaacg ggagccgata
     2041 cattaaagta ctaccagaaa gccctggagg cccgctctaa ggttattaac ggcgaaccct
     2101 ttggcaaagt tgcattaacc acaagtaacg acccctcggt tagccgaaac aatggctacc
     2161 taggttggtt ctcggccttt caaatggttt acccctttga aaccgcagca tacagtactc
     2221 cggtcgattc ggtttcgatg ccggttcgca cccgctttgg ttaccatatc attaaggttc
     2281 acggtcgtcg ccctgccaag gggcaaatta aggttgccca catcatggtg agggttaacc
     2341 ccaatgcctc gcctcaggaa atggaaaacg cccgccaaaa aatagt
//
LOCUS       DAOOWJ010000207         2288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_47064_length_2288_cov_3.630990, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000207 DAOOWJ010000000
VERSION     DAOOWJ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47064_length_2288_cov_3.630990"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLW22_11990"
     CDS             <1..296
                     /locus_tag="PLW22_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75466.1"
                     /translation="AELPPTFSKLKPTVKEIRKWEEGFNHLKDIDTNALGIIISARKG
                     TIAYNNDIQNTLLLTSQIISDKNIVIIYPAIHGTSIIDSRSLLSGTGRVVAEN"
     gene            complement(258..1934)
                     /locus_tag="PLW22_11995"
     CDS             complement(258..1934)
                     /locus_tag="PLW22_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOU75467.1"
                     /translation="MERNRAVYKLSPLWQRAIIGGSIWGALEIILGSALHNLMVPMVA
                     GTLLAFLGVFTVSVISANDIQRGFFWRAALICALLKSVSPSAVIVPPMVGIMVEGLLM
                     EVGVLVLGANAFGLALGGGLAMLSIPLFKAVRLYMLYGQGIVDFFMGLISQVSKSNTI
                     VITNALIYTILIIYLILGLLAALIGFSLGLKGSTFGSSQIELTIFGEKRGKVAFSNYL
                     LLIIHLVALVAYLTYASAMPFAIAILSGGVYVLLIVLFYERPRRMLLKPFFIVPVLVF
                     SFLIPFFTTKIALVPVYGIYIFVRAIFVVVTLAAIGTELAKPAVSQFFNRGFFSPVYY
                     ASSMAFNALPIYLNVFRNINFSASNAIKDIQGVIKNSGWSGNRPIIIITGGLGEGKST
                     YLENILKILGKDKAINFRGFIAMGIGAPPLREGYNLRIIPDGNEMMLCHRIGACGLPN
                     KSFEFNDEVIKGLTSELVSIKADDILVIDEVGRMELYGEVWAELIEHHLTKTKNVLVL
                     TVRRENLMHVVERWNLTDAYIFDINKVSENDAANSIKSLVLSYHAARSRK"
     gene            complement(1921..2262)
                     /locus_tag="PLW22_12000"
     CDS             complement(1921..2262)
                     /locus_tag="PLW22_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75468.1"
                     /translation="MGKIRISERKLILFTVGLLIVAGIVRLGFYKVGVWLFYLAFVPI
                     ILHRLRYYIRNSKNLHKADRYRRFTLGFIIATIILNLLGFQDVEFVLLFVLAIDYLIV
                     VQNPRVKGGKE"
BASE COUNT          790 a          528 c          360 g          610 t
ORIGIN      
        1 tagcagaact tcctcctaca ttcagcaagc taaaaccaac cgtaaaggaa atacggaaat
       61 gggaggaagg atttaaccac cttaaagaca tcgatactaa cgcactagga attataattt
      121 ctgcccgtaa aggcacgata gcctataaca acgatattca gaacactctt ctacttacca
      181 gccaaattat ctccgataag aatattgtaa ttatctaccc cgctattcat ggaacatcaa
      241 taatcgattc ccgatcacta ctttcgggaa cgggccgcgt ggtagctgag aactaaactt
      301 ttaatactat tggcagcatc gttttcacta accttattga tatcgaatat ataggcatcg
      361 gttaggttcc accgctccac tacatgcatt aggttttcgc gccttaccgt taatactaaa
      421 acattcttag ttttagtaag atgatgttct atcagctctg cccaaacttc accataaagt
      481 tccattcggc caacctcatc aattacaagt atgtcatcag cctttattga aacaagttcg
      541 gaagttagcc ccttaataac ctcatcatta aactcaaatg atttatttgg caagccacaa
      601 gcaccaatac gatggcacaa catcatttca ttaccatctg ggattatccg taaattatac
      661 ccctctcgta gtggaggcgc accaataccc atggcaataa atcctctaaa gtttatcgct
      721 ttatcctttc ccaaaatttt cagaatattt tcaaggtagg ttgatttgcc ctcgcctaac
      781 ccgcctgtaa tgattataat aggcctattt ccactccaac cggaattttt tattacaccc
      841 tgaatatctt taatagcgtt tgatgccgaa aaattgatgt tcctgaaaac atttaggtaa
      901 ataggcaatg cattgaatgc catactggag gcatagtaaa ccgggctaaa aaatcccctg
      961 ttaaaaaatt ggctaaccgc tggtttggct agttcagtcc caattgcagc tagggtaacc
     1021 acaacaaaaa tagcccttac aaaaatatag ataccatata ctggcactaa agcaattttg
     1081 gttgtaaaaa atgggattaa gaaagaaaag accaaaacag gcacaataaa gaaaggtttc
     1141 aatagcattc ttcgtggtct ttcgtaaaat aaaacaatta acaaaacata tacccctcca
     1201 ctcaaaattg caatagcaaa gggcattgcc gaggcataag taaggtatgc aaccagtgca
     1261 acaagatgaa taatcagtag taaatagtta ctaaaagcta ccttccctct cttttctcca
     1321 aaaatagtca gctcaatttg acttgaacca aaagtggaac cttttagccc taaactaaag
     1381 ccgattaaag ccgccagtaa gcctaaaatc aagtagatga ttagtatagt ataaattaat
     1441 gcgttggtaa taacaatggt attacttttt gatacttggg aaatcaaacc cataaagaaa
     1501 tcgactattc cttgaccata aagcatgtaa agtcgaactg ctttaaaaag gggtatagag
     1561 agcatagcca aaccaccgcc taacgctagc ccaaaagcat ttgctcccaa aaccagcacc
     1621 ccgacctcca tcaatagccc ttcaaccata attcccacca taggcggtac gataacagca
     1681 cttggcgata ccgactttag taaggcacag attagtgcag ccctccaaaa aaatcccctc
     1741 tgaatatcgt tagccgaaat aaccgaaacg gtaaaaactc caagaaatgc caacaatgtt
     1801 ccggccacca tgggtaccat aaggttatgt aatgccgaac ctaagatgat ttccagtgcg
     1861 ccccaaatgc taccaccaat aatggcacgc tgccacaacg ggctaagctt atatactgct
     1921 ctattccttt ccacctttaa cccttggatt ttgcacaaca atcaagtagt caatagctag
     1981 aacaaaaagc aaaacaaact ctacatcctg aaatcctaaa aggtttagaa taatagtagc
     2041 aataataaac cccaacgtga accgtctata cctgtctgct ttatgtagat ttttgctatt
     2101 cctgatataa tacctcaatc tatgtaaaat aattggtaca aatgccaagt agaaaagcca
     2161 tactcccact ttgtaaaatc ccaatcgaac tattcctgcc actataagta aaccaacagt
     2221 aaaaagaatt agtttccgct ctgatattct gatttttccc attgcttaaa aaattggtac
     2281 caaaattg
//
LOCUS       DAOOWJ010000208         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_47265_length_2281_cov_3.238095, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000208 DAOOWJ010000000
VERSION     DAOOWJ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47265_length_2281_cov_3.238095"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="PLW22_12005"
     CDS             complement(<1..1906)
                     /locus_tag="PLW22_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU75469.1"
                     /translation="MRRVILLLLSVLVAGLQLLTAQVAKQQITLDDLFKKGTFRANSI
                     WGLSPMNDDEFYTAYNDKRQVAKFKLATGEQVDVLFDPSAFDVADLKAMSGYQLSADE
                     TMMMVETNPQPIYRYSYKAENYIYNIKTKELKRLSENGPQMNATFSPDGSKVAFVRSN
                     NIYIVDLQNFEEIQVTTDGEYNSIINGAADWVYEEEFALTNGIQWSPDSKKLAFYRFD
                     ESRVKMFNMTMYGRNLYPENYSFKYPKAGEENSIVTIHIYDAIGKTTRQMDAGPNSDQ
                     YIARIKWMPTSDKLCMIRLNRLQNQIDILMADAATGNSEVVFTETNKYYIDEVTDDYL
                     TITPDGKQFILTSERDGFKHIYLYDWQSKAIQQITKGSSEVISVYGFNSKDKTIYYLG
                     YDQSPLRRAVFAINLKGKTQKLSPNSGLNSAVFTQNYKYYMVYHSSITSPLSVTLYKG
                     NGKQVRVMEDNQKLREKLLLYDIPKKEFFTFKTSEGVELYGYMVKPLNFDSTKRYPVM
                     MYQYSGPGSQAVIDNFRIGWDEYLATLGYMVVCVDGRGTGGRGEQFKKMTYGQLGYYE
                     SIDQIETAKYLQSLPVVDGSRIGIWGWSYGGYMSSLCLFKGADLFRMAIAVAPVTNWR
                     YYDSIYTERYM"
BASE COUNT          638 a          488 c          484 g          671 t
ORIGIN      
        1 ccatgtagcg ctcggtgtaa attgagtcgt aatagcgcca attggtaacg ggtgccacgg
       61 ctattgccat tcgaaaaaga tcagcaccct tgaaaaggca aaggctcgac atgtaaccac
      121 cgtaactcca cccccaaata ccaatgcggc ttccatcgac aaccggaagg gattgcaggt
      181 atttagccgt ttcaatctga tcgattgatt cgtagtagcc aagctgcccg taggtcatct
      241 tcttgaactg ctcacctcgg ccgccagttc cgcgtccatc cacacaaaca accatatacc
      301 ctagggttgc caagtactca tcccaaccaa tacggaagtt atcgataacc gcctgggagc
      361 caggaccact atactggtac atcatcaccg ggtaacgctt ggtgctgtca aaattcaagg
      421 gttttaccat gtagccataa agttctactc cctctgatgt tttgaaggtg aaaaattcct
      481 ttttgggaat gtcgtatagc agcagttttt ccctaagttt ttggttatcc tccattaccc
      541 tgacctgctt accatttccc ttgtaaaggg ttacactcaa tggcgatgta atggaagaat
      601 gatataccat atagtactta tagttttgag taaaaacagc actattcaac ccactgttgg
      661 gcgataactt ttgcgtttta ccttttagat taatggcaaa aacagccctc cgtaaaggac
      721 tttggtcgta tccaaggtag tatatggttt tatctttgga attgaatccg taaactgaaa
      781 tcacctcgct tgatcccttg gtgatttgct gaatggcctt gctttgccag tcgtaaaggt
      841 agatatgctt aaagccatcg cgctccgagg taaggatgaa ctgtttcccg tcgggggtaa
      901 tggtaaggta atcgtcggtt acctcatcga tatagtactt gttggtttcg gtaaaaacaa
      961 cctcggaatt acccgttgct gcatcggcca taagaatgtc tatctggttc tgtagcctgt
     1021 tcaacctaat catgcaaagc ttatcggatg tgggcatcca cttaattcgg gcaatgtact
     1081 ggtcactatt agggccggca tccatttggc gggtggtttt gccaatggca tcgtaaatat
     1141 ggatagtaac tattgagttc tcctcacctg ccttaggata cttaaagcta tagttttcag
     1201 ggtaaaggtt acgtccgtac atggtcatgt taaacatctt tacccggctc tcatcaaagc
     1261 ggtaaaatgc aagttttttg gaatcgggcg accactgaat tccgttagta agggcaaact
     1321 cctcctcgta aacccagtcg gctgcaccgt taattatact gttatactcg ccatcggttg
     1381 tcacctgaat ttcttcgaag ttttgcagat caacaatgta gatattatta ctacgaacaa
     1441 acgcaacctt actaccatcg ggcgaaaagg tggcattcat ctgcgggccg ttctcagaca
     1501 atcgttttaa ctccttggtt ttaatgttat agatataatt ttcggcctta taggagtagc
     1561 ggtagattgg ttgtgggttg gtttcaacca tcatcatagt ttcatcggcc gaaagctgat
     1621 agcccgacat tgcctttagg tcagcaacat caaacgccga tgggtcaaaa agaacatcta
     1681 cctgttcgcc tgtggcaagt ttgaactttg ccacctgcct tttgtcgttg taagctgtgt
     1741 aaaactcatc gtcgttcatg ggcgataacc cccaaatgct gtttgcacgg aatgtcccct
     1801 ttttaaaaag atcgtcaagg gtaatttgct gcttggctac ctgtgctgtt aaaagctgaa
     1861 gcccggcaac taaaactgaa agcaacaaaa gaattactct tctcatacgc attggttttg
     1921 gttttcaatt ttgatatcaa agtaagccaa atgtttaaat ccgattagta agtaacacgg
     1981 ttttaaccat ggcactaatg taggaataaa aaattgataa gaaaatgata agccgtaata
     2041 gttctaaatc gattgcggat gttaaaaggt gtgaaaacct aacttttgtt cccctattcc
     2101 atttttttta atacttttgt atgttcacga agaatttgtt gaaataaagt aaaataaacc
     2161 ctttaagttt aattaaaaac tcgaaaatag atgtcgaaag taaagagagt ttacgtcttt
     2221 gggaataaga cggccgaagg aaaagcagac atgaagaatc tgcttggcgg taagggtgca
     2281 a
//
LOCUS       DAOOWJ010000209         2074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_53796_length_2074_cov_7.457157, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000209 DAOOWJ010000000
VERSION     DAOOWJ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53796_length_2074_cov_7.457157"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..758)
                     /locus_tag="PLW22_12010"
     CDS             complement(183..758)
                     /locus_tag="PLW22_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOU75470.1"
                     /translation="MRICVYCASSSKVDNKYFEATKVLAKDFVSKDVTIIYGGGSYGL
                     MGCLADTALEHGGRVIGILPRFMEKVEWGHKNLTQLVLVKDMHERKKLLIQDVDAVVA
                     LPGGCGTLEELMEVITLKRLGKFTKPIVILNTDNFYYHLKEMLQKMIDERFMRNEHNA
                     IWQFVNNPEDVIPAILNAPHWDSDAINFAAV"
     gene            complement(825..1142)
                     /gene="trxA"
                     /locus_tag="PLW22_12015"
     CDS             complement(825..1142)
                     /gene="trxA"
                     /locus_tag="PLW22_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU75471.1"
                     /translation="MALAVTDANFDELVLNSGKPAMVDFWAEWCGPCRMIAPYVEQMA
                     EEYKDKAVVAKVDVDSNPGIAARYGIRNIPTVLFFKNGQMADKQVGAVPKSALVAKLE
                     ALL"
     gene            complement(1217..>2074)
                     /locus_tag="PLW22_12020"
     CDS             complement(1217..>2074)
                     /locus_tag="PLW22_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOU75472.1"
                     /translation="VAGAFDNLIDFDRSVLLAVDAKGVSYLEQLMRYGIKVQSEKNTT
                     QQSLFGTVPGFEVPKPAPPKVDPWPTIEKLNKEKEVIGIYLSAHPLDEFRFEIESLCN
                     VSIADLKSNMEQYRDRDVTIAGMTIASRIDTAKNGKQYGRITLEDYTDSIDIMLFGKD
                     FESYRNFCFNGYYIMVRGKVQPKAYKPEELELHIKSINMLYDVREKMIKSITLNLPLD
                     EINDIFIDDILKYVQRDKANITLKFKVYDPEYQVSVDLFSRAYRVNITQDFIDYLNDS
                     EINYKVAME"
BASE COUNT          595 a          466 c          427 g          586 t
ORIGIN      
        1 agctttattc agaaaaacct ctgggacgaa gcccgaatgt ttgtgggcaa taagttcttc
       61 ggcgagggag ttaaagcccc taccctcaag ggtaaaatgg taacgtacga tgaaattggc
      121 gacagccgat tgtttgttta tagaaatgag taattactga atagctaagt ttgcataaac
      181 cctcaaaccg ctgcaaagtt aattgcatcg gagtcccaat ggggggcatt cagaattgct
      241 gggattacat cttccggatt attgacaaac tgccagatgg cattatgctc gttgcgcata
      301 aagcgttcgt caatcatttt ttgaagcatt tcctttaggt gatagtaaaa gttatcggta
      361 tttagtatta caatgggttt ggtgaattta ccgagccgtt taagagttat aacctccatt
      421 agctcctcaa gggtaccaca acctcctggc agggcgacaa cggcatcaac gtcctgaatg
      481 agtaattttt tacgctcgtg catatccttt accagcacaa gttgtgttag gttcttgtgc
      541 ccccactcca ccttttccat gaatcggggc aagataccta ttacccttcc gccatgctct
      601 agggcagtat cggcaaggca gcccataagc ccataggatc cacctccata aattattgtt
      661 acatctttac taacaaaatc cttagctaaa accttagttg cctcaaagta cttattatcg
      721 acctttgaac tagaagcgca gtaaacacaa attcgcatag ctatcaaatt aaaaaaaact
      781 aaagccgggt aaagcacacc cggcttgtat gatgattgaa atacttacag caaagcctcg
      841 agcttagcaa caagagcgct cttgggtaca gcgccaacct gcttatcggc catttgtccg
      901 ttcttaaaga aaagcaccgt tgggatattt cggatgccat agcgtgcagc aatgccaggg
      961 ttgctgtcta catcaacctt tgctaccact gctttatcct tgtactcctc ggccatttgt
     1021 tcaacatagg gagcaatcat acggcagggg ccacaccact cagcccaaaa atcaaccatt
     1081 gcgggttttc cggagttgag cacaagctca tcaaaatttg cgtcagttac tgctaatgcc
     1141 atagtttata tgttttaaga tagatttgta gatatttaaa caatcacaaa tttaaaccaa
     1201 aaaaatgata aaaggtttac tccatggcaa ctttgtagtt aatctcgcta tcgttaaggt
     1261 aatcaataaa atcctgagtt atgttcacgc ggtaagccct ggagaataaa tccacagaaa
     1321 cctggtactc cggatcgtaa accttaaatt ttagggtgat attagcctta tcgcgttgca
     1381 catacttcaa aatatcgtca ataaaaatat cgttaatctc atcgagggga aggttaagcg
     1441 taatgctctt tatcatcttt tcacgcacat cgtatagcat gtttattgac ttgatgtgta
     1501 gctcaagttc ctcgggctta taggctttag gctgaacctt acccctgacc atgatgtagt
     1561 agccgttaaa gcaaaagttc cggtagctct caaagtcctt accaaagagc atgatatcaa
     1621 tggaatcggt gtaatcttca agggttatgc gcccatactg tttcccattt ttagctgtat
     1681 cgattcgcga ggctatggtc atgccggcaa tggttacatc cctgtcgcga tactgttcca
     1741 tgttggactt aaggtcggca atggacacat tgcaaaggct ttcaatttca aaacggaact
     1801 catcgagcgg atgggctgaa aggtaaatac caatcacctc cttttccttg ttcagctttt
     1861 caatagtagg ccagggatct accttgggcg gtgcaggttt aggaacctca aagcctggaa
     1921 ccgttccaaa aagcgattgc tgggtagtat tcttttccga ttgtaccttt attccgtagc
     1981 gcataagttg ctcaaggtac gatacaccct tagcatcaac tgccaatagt acactgcggt
     2041 caaaatcaat aagattatca aaggcgccgg caac
//
LOCUS       DAOOWJ010000210         2063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_54179_length_2063_cov_6.677291, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000210 DAOOWJ010000000
VERSION     DAOOWJ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54179_length_2063_cov_6.677291"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLW22_12025"
     CDS             complement(<1..644)
                     /locus_tag="PLW22_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOU75473.1"
                     /translation="MQRGPLFGIKVLELANVLAGPSVGVALAEMGASVIKVENSVTCG
                     DVTRTWKLTTEDPKTDISGYFSCVNWGKKSIALDLNKPEGLEVVYRLAKVCDIVLASY
                     KPGDAEKLKVDYNTLKQHNKSIIYAQITGYGLKNKRAGYDAIIQAESGFTYMNGEPDG
                     QPVKMPVALMDLLAGHQLKEAILLALLNRERKGEGAFIETSLIKSGVAALANQAT"
     gene            complement(661..>2063)
                     /locus_tag="PLW22_12030"
     CDS             complement(661..>2063)
                     /locus_tag="PLW22_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOU75474.1"
                     /translation="AYFAIVVGVSFLTRKVASRSVADYLVAGRNLGIMLCSVVVAAEW
                     LGGMSTIGVSEKAFQKLSFQPILYNVATSIGMIIIGFTVAKHYRKNNVHTVSEMLEHL
                     FGIHAKRVSAIAFLIAYITLAYVQLQTATSVMSSIFQIDWLTSVIIGAGVITLYTYIG
                     GMHALAITSIVHLVVMFVGLGTAMVIGVVKIGGFAHLQELLAAKGAVGSIYNPFGVSF
                     NEAFSLLLGGVLGGMAAQASIQPIFAARTPEVAKKASILSSAYIAPFGVMTAILGLIA
                     ATGVYEIGASAPSFSAKVAMTNLLVNPQFIHPILGGLALAGILAAILSTVGPVNFAVV
                     TIATKDIYHGFINPEADEQKVLQIARRLVIIVSLITIPLAYYFKAGVLDSAYISYAIR
                     AIGAIVIIAGIYFRGWIDVFTVKLAFIGGTIAVFLCIVADKLKWFHLDKTYGAVLFAL
                     AALVFGYIRRKFFVKE"
BASE COUNT          632 a          481 c          406 g          544 t
ORIGIN      
        1 gtagcctgat tggccagagc agcaacacct gattttatca ggcttgtttc aatgaaagct
       61 ccttcacctt ttctctcccg gttaagtaaa gccagcaaaa tggcctcttt aagttgatgt
      121 ccagccagta aatccataag ggctacgggc atcttaacgg gttggccatc gggctcgcca
      181 ttcatatatg taaaaccgct ttcggcctga atgatagcat cataaccggc acgtttattt
      241 ttaagaccat agcctgttat ttgggcataa ataattgact tgttatgttg ctttaaggtg
      301 ttgtaatcta ccttgagctt ttcagcatcg ccgggcttgt agctggctaa aacaatatcg
      361 caaaccttgg ctaggcggta aactacctca agcccttcgg gcttattcaa atcgagagct
      421 attgactttt tgccccagtt cacgcagctg aagtaacccg aaatatcagt cttagggtct
      481 tcggtggtta acttccaggt tcgtgtaaca tcgccgcatg taaccgagtt ttcaacttta
      541 attacgcttg cgcccatctc ggctaaagcc actccaacac ttggcccggc caaaacgttg
      601 gccaattcaa gcacttttat cccaaatagt ggacctcgtt gcattatctg cgagtgttaa
      661 ttactccttt acaaaaaact ttctgcggat atatccaaat actaaggcag ctaaagcgaa
      721 aagaactgct ccataggttt tatcgagatg gaaccatttt agcttatcgg ctacaataca
      781 taggaatacg gctattgtac ctccgataaa tgcaagtttt acagtaaaaa catcaatcca
      841 tcccctgaaa taaataccgg caataattac aattgcacca atagcacgaa ttgcatagct
      901 tatataggct gaatcgagca ctcccgcctt aaagtagtat gccagtggaa tagtaataag
      961 gctaacaatg ataaccagtc gacgagcaat ttgcagtacc ttttgctcat cggcctcagg
     1021 gttaataaaa ccatgataga tatcttttgt agcaatggta actacagcaa agttaacagg
     1081 accaacagtt gagagaattg ctgccaaaat acctgccaga gccaatccac ccaaaatggg
     1141 atgaatgaac tgtgggttta ccaaaaggtt agtcattgca acttttgctg agaaagaagg
     1201 agcgctagcg ccaatttcat aaacaccagt agctgcaatt agacccagta tagcagtcat
     1261 taccccaaac ggagcaatat atgcactgga tagaattgaa gcttttttgg caacttcagg
     1321 ggttcgggca gcaaaaattg gctgaatgct tgcctgagcg gccattccgc caagaactcc
     1381 tccaagaagt aaactgaaag cctcgttaaa gctaacacca aaggggttat agatgcttcc
     1441 caccgcacct ttagcggcaa gcaattcctg tagatgtgca aaacctccaa tttttacaac
     1501 acctattacc attgctgttc caagtccaac aaacataact acaaggtgaa ctattgaggt
     1561 aatggctaaa gcatgcatac ctccaatgta ggtataaagg gtaataacac ctgccccaat
     1621 aattactgag gtgagccaat caatctggaa aattgagctc attacactgg ttgcggtttg
     1681 gagctgaacg tatgccaaag ttatgtatgc aattaaaaat gcaattgccg aaaccctttt
     1741 agcatgaatg ccaaagagat gctccagcat ttcacttaca gtatgaacat tgtttttacg
     1801 gtaatgctta gctacggtaa aaccaatgat tatcatacct attgatgtag ccacgttgta
     1861 aagaattggc tgaaacgata gcttctgaaa agccttttcg ctaacgccaa tagtgctcat
     1921 accgcctagc cattcagcag caacaaccac tgaacatagc ataattccaa ggttccgtcc
     1981 cgccaccaag taatcggcaa ccgaacgcga tgccactttc cgggttaaaa acgatacccc
     2041 tactactatt gcaaagtatg caa
//
LOCUS       DAOOWJ010000211         1961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_58150_length_1961_cov_3.181007, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000211 DAOOWJ010000000
VERSION     DAOOWJ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58150_length_1961_cov_3.181007"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLW22_12035"
     CDS             complement(<1..650)
                     /locus_tag="PLW22_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU75475.1"
                     /translation="MDKIRVALVEDHQLVRDGIRSLLTNLPNIEVVAEADCAKTMLNL
                     IEQARPNVLLVDISLPEISGIELAKLITTNHPGIKVIILSMHTEQEFIFNSLRNGAKG
                     YLHKSISREELIEAIEQVYNGSEYFSKEVSGIILKSYLRQIKNPERVEEYEDKKLTPR
                     EMEILRMVAQGYSNQLIADKLFISVRTVESHKNHIMQKLELTTTVDLVKYAIKNGI"
     gene            complement(643..>1961)
                     /locus_tag="PLW22_12040"
     CDS             complement(643..>1961)
                     /locus_tag="PLW22_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOU75476.1"
                     /translation="INNRVSVLEFQKALASLFKVNIGNLLLKAIVFLIVISLVIFVVM
                     ALQAKLRSIALANELNLVEKSIVENHDISESKVQLVSIVKPLIHQLRFRFQELKLQHH
                     EVSDRLNQFYRASVDAIVIHHDGIPIFFNPAFPEMTGYPESALYRLRLFDVIKVDEDL
                     LKEARDARQPSVEGLLRQSNGNTLYVEVQVKSIKFRGQVAESVVIRDITHRKFMEREL
                     QLQRMRQVKSVIDGQEKERQRLSRELHDGLGQNLVAIKLKLESIPHDMAGELNSTLTQ
                     VKQMFNHTIEEVRRISNNLMPAALKEFSLAVVLRNLCNEVENNSGISVGLTVGVLPEP
                     IDQLLKTYVYRIIQEALTNVIKHSGASRANITVYADFSNLHLIIEDNGVGFNTAIPTD
                     TGNGLYNMKERAILLNGSINIISSEGKGTKIVAQFPLKPQNQSVNG"
BASE COUNT          504 a          463 c          413 g          581 t
ORIGIN      
        1 ataattccat ttttaatggc atacttaacc aaatcgacag tagtggttaa ctccaacttt
       61 tgcatgatat gatttttatg ggactccacg gttctaacgc tgataaaaag tttatcggca
      121 ataagctgat ttgagtatcc ctgtgcaacc attcgaagta tctccatctc gcgaggtgtt
      181 agctttttat cctcgtattc ctcaaccctt tcggggtttt tgatttgcct gagatagctt
      241 ttaaggatta tacccgacac ctctttgcta aagtactcac tcccattgta aacctgctca
      301 atggcttcga taagttcctc gcgcgatatg ctcttatgca ggtagccctt ggcgccattg
      361 cgaagcgaat tgaatatgaa ctcctgctct gtatgcatgg aaaggattat caccttaata
      421 cccggatggt tcgtggtaat aagctttgcc agctctatgc ccgatatttc gggaagcgaa
      481 atatcaacaa gcagcacgtt aggccttgcc tgctcaatca ggttcagcat ggttttggca
      541 caatcggctt cggcaaccac ctcaatgtta gggaggttag tcaatagcga ccgaatgcca
      601 tcgcggacta actggtgatc ctcaaccaaa gctactctta ttttatccat ttaccgattg
      661 gttttgtggt tttaatggaa actgtgcaac tattttggtg cctttccctt cggacgaaat
      721 gatgttgata cttccgttca gcagaattgc cctttctttc atgttataca atccgtttcc
      781 ggtatcggta ggaatggcag tgttaaatcc cacaccattg tcctcaataa ttaggtgaag
      841 attgctaaaa tcggcataaa ccgtaatgtt tgccctactg gcgccggaat gcttaatcac
      901 attggtaagg gcttcctgaa taatcctgta aacataggtt tttaaaagct gatcgatagg
      961 ttcgggcaga actccaacgg taagccccac gctaatcccc gaattgttct caacctcgtt
     1021 gcaaagatta cggagcacaa cggcaagact gaactccttc aatgcagcag gcataaggtt
     1081 gttggatata cggcgaacct cctcgatggt gtggttgaac atttgcttta cctgcgtaag
     1141 agtactgttc agttcaccag ccatgtcgtg gggtatgctc tcaagcttta gcttaatggc
     1201 taccaggttt tgccctaacc catcgtgaag ctcgcgggac aagcgctggc gctccttttc
     1261 ctggccatca ataaccgatt ttacctgtcg catcctttgg agctgtagct cccgctccat
     1321 aaacttccgg tgggtgatat cccttatcac aaccgactcg gccacttgcc ctctgaattt
     1381 aatcgatttt acctgaacct caacataaag ggtattccca ttgctttggc ggagcagccc
     1441 ttcaacgctt ggttgccgag cgtccctggc ttccttaagt aaatcctcat caaccttaat
     1501 aacatcgaaa aggcgaagcc tgtaaagggc actctcggga tatcctgtca tttcaggaaa
     1561 agcagggttg aagaagattg gaatgccatc gtgatgtata acaatggcat cgaccgatgc
     1621 ccggtaaaac tggtttaacc tatcgcttac ttcatggtgt tgtagtttta gctcctggaa
     1681 cctaaacctc aactggtgta tcaatggctt aacgattgaa acgagttgta ctttgctttc
     1741 gctgatatcg tggttctcaa ctatgctttt ctcaactaaa ttcaattcgt ttgccaaagc
     1801 tattgatctt agcttagcct gtaaggccat gaccacaaaa atcaccagtg atataactat
     1861 tagaaagaca atggccttca agagtaagtt gccaatgttg accttgaaaa gcgaggctaa
     1921 tgctttttgg aactccaaaa ccgatacccg attattgatg g
//
LOCUS       DAOOWJ010000212         1953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_58461_length_1953_cov_4.791886, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000212 DAOOWJ010000000
VERSION     DAOOWJ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58461_length_1953_cov_4.791886"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLW22_12045"
     CDS             complement(<1..509)
                     /locus_tag="PLW22_12045"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HOU75477.1"
                     /translation="MDELRVVPTADGSPTLYSERFNATYHSVNGALTESMHVFIKSGF
                     EAILLNKKTVEVLEVGMGTCLNAALTAWVAPKFGVKVHYSGIELFPPILEVLNSTGYQ
                     TVLEPECADLWVKIVAASAGDPVPVSPWFTVFKLYVDFISWIPNQRYDLIYFDAFAPD
                     DQPEMWSIE"
     gene            complement(585..1184)
                     /locus_tag="PLW22_12050"
     CDS             complement(585..1184)
                     /locus_tag="PLW22_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU75478.1"
                     /translation="MKRLFLMLMATFMVAGLYAQGLKIGDKAPDFRLKNVDGKMVSMA
                     DYPQAKGFIVIFSCNHCPFVKAYEDRMIALHQKYESKGFPVIAINSNDPEVVPEDSFD
                     KMIERAQQKKFPFVYLFDEGQKIYPLYGATRTPHVYILSKKANDLVVEYIGAIDDNYK
                     DASAVKEKYVENAVDALLAGKKPAVTETKAVGCSIKVKK"
     gene            complement(1198..1701)
                     /locus_tag="PLW22_12055"
     CDS             complement(1198..1701)
                     /locus_tag="PLW22_12055"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU75479.1"
                     /translation="MLRSFILIIIVIVLVGCNPNRHTYSYDTIDVVDFKMLEPQFTKS
                     TDTVYVVNFWATWCSPCVKEFPYFDEAAKIYSGRKVKFLMVSLDFPNQVEKSLKPFVK
                     SRTNRLPVVLLDDPNQNEWINKVSPDWSGALPATLIYKGSNKVFIEGPIELTDITSNI
                     DSLLVKL"
BASE COUNT          596 a          413 c          358 g          586 t
ORIGIN      
        1 ttttcaatac tccacatctc gggctggtcg tcaggagcaa aagcatcaaa gtaaatcaaa
       61 tcataccttt ggttaggaat ccagcttatg aaatcaacat acaacttaaa tactgtaaac
      121 cagggagata ctggaactgg atcgccagca gatgcagcaa caatttttac ccaaaggtct
      181 gcgcattctg gttctaatac agtttggtaa cccgtagagt ttagcacttc aagaattggt
      241 gggaaaagtt caataccgct gtaatgtacc tttaccccaa actttggcgc aacccatgca
      301 gtaagagctg cgtttaaaca ggtgcccatt ccaacctcaa gcacttcaac agttttctta
      361 tttagcaaaa tggcttcaaa acctgatttt atgaatacat gcatcgattc cgttagcgca
      421 ccgtttacag aatggtaagt tgcgttaaac ctttcgctgt atagcgtggg gctgccgtcg
      481 gcagttggaa ctacccttaa ttcatccata gcagattatt taggcaaagc taataaaaaa
      541 gccgctagca ttatagcggc ttaatcagta ttgtcggcat taaactattt tttcactttt
      601 attgagcagc ctactgcttt agtttctgtt acggcaggtt tcttaccagc aagtaaagca
      661 tcaacagcat tttcaacata tttttccttt acggccgagg catctttata gttatcgtca
      721 atagcaccaa tgtactcaac aaccaaatcg tttgcttttt tactcaggat ataaacatgg
      781 ggtgtacggg ttgctccgta aagcggataa attttttgtc cctcatcaaa gaggtaaaca
      841 aatgggaatt tcttttgctg agcccgttca atcattttat cgaacgaatc ttccggtaca
      901 acctcaggat cgttagagtt gatggcaatt accggaaacc cttttgattc gtacttttgg
      961 tgtagggcta tcattctatc ctcgtaggct ttcacaaaag ggcaatgatt acaagagaag
     1021 ataacgataa acccctttgc ctgggggtag tcagccattg aaaccatttt gccatcaaca
     1081 tttttcaggc gaaagtcagg ggccttatcg ccaattttta aaccctgagc gtatagcccg
     1141 gccaccatga aagtagccat aagcattaaa aacaatcgtt tcatagattc attcgtttta
     1201 aagtttgaca agtaaactat cgatatttga agtgatatcg gttaattcaa taggcccttc
     1261 aataaaaact ttattgctgc ctttgtatat tagggttgca ggaagtgctc cgctccagtc
     1321 ggggctaacc ttattaatcc attcattttg attgggatcg tcgagcaaaa ctacaggcaa
     1381 acggttagtt ctggatttta caaatggctt caagctcttt tcgacctgat tgggaaaatc
     1441 gaggctaacc attaagaatt taaccttgcg gccggaatat atttttgcag cttcatcaaa
     1501 gtatggaaat tccttaacgc atggcgaaca ccaggtagcc cagaagttaa ctacgtaaac
     1561 cgtatcggtt gattttgtaa actgcggctc aagcatctta aaatcaacaa catcaatggt
     1621 gtcgtaacta taagtatgcc tgttggggtt acagccaacc aaaactatca caataattat
     1681 taaaataaac gatctcaaca tcgctcagct atttttaaat taacaaacaa aaaacttctt
     1741 tgttcaaata ccgtttattt tgcacgccac ccaattaatg gtaataacaa tttggtaaaa
     1801 aacaatggaa gtaataaatt tgatgtttaa aactcatttt ggtttagatg ataagagcca
     1861 taaaaaatac tttccttttt tttaccacca tcataatctc gggctgttcc ttagtaccca
     1921 ccgcaccttt agccgaacaa cacgatacgc ttc
//
LOCUS       DAOOWJ010000213         1936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_59205_length_1936_cov_3.515683, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000213 DAOOWJ010000000
VERSION     DAOOWJ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59205_length_1936_cov_3.515683"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLW22_12060"
     CDS             complement(<1..355)
                     /locus_tag="PLW22_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU75480.1"
                     /translation="MVKNRKLLNEELERKSIDEFKTSEKLPIAVVLDNVRSLNNVGSV
                     FRTSDAFLVEKIFLCGITGTPPHPEIHRSALGAEKSVEWEYVESTMKAVEGLKQSGYV
                     IISVEQVEHSVLLNQF"
     gene            430..1617
                     /gene="sucC"
                     /locus_tag="PLW22_12065"
     CDS             430..1617
                     /gene="sucC"
                     /locus_tag="PLW22_12065"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOU75481.1"
                     /translation="MNLHEYQSKEILRQAGVPVPVGLVAFTKDEAVRAAQEVMKLTGT
                     DTIAVKAQIHAGGRGKGGGVKIAKTVDEVATYASNIIGMNLVTHQTSPQGKLVRKVLI
                     EQGIYQPGPSEIKEYYMSILLNRATGRHMVIYSPQGGMDIEAVAAQTPELIFKEEIHP
                     QGLQAFQARKIAFNLGLSGKAYQQMVKFVMSLYEAYTKNDLSLLEINPVVKTSNDDIL
                     AADAKCSIDDNALYRHPDLAEYRDINEEDPAEVEAGEHNLNFVKLDGNVGCMVNGAGL
                     AMATMDIIKLSGGDPANFLDVGGGANAKTVAAGFRIILKDSNVKAILINIFGGIVRCD
                     RVAQGVVDAYKEIGNIPVPVIVRLQGTNALEGKKMIDESGLKVYSAITLQEAANLVKQ
                     MVN"
BASE COUNT          590 a          413 c          399 g          534 t
ORIGIN      
        1 aaaactggtt aagcagcacc gaatgctcca cctgttctac tgaaataatc acataccccg
       61 attgtttcag tccttcaact gctttcattg tactttcaac gtactcccat tcaaccgatt
      121 tttcagcacc cagtgctgat cgatgtatct cagggtgcgg tggagtaccg gttataccac
      181 ataggaatat tttttcaacc aaaaaagcat cagatgttct gaaaactgaa cctacattat
      241 ttaaactcct aacgttatcg agaactacag caattggcaa tttttcagag gttttaaatt
      301 catcaatcga ttttcgctcc agctcttcgt ttaacaactt tctgttttta accatatcca
      361 caaaaaattt accaaattta gttaatcctt tttttgtaac ttagatgcat aaaaaaatga
      421 tattattcta tgaatctaca tgagtatcaa tcgaaagaaa tactaaggca agcaggagtt
      481 ccagtgccag taggtttagt agcctttact aaagatgaag ctgtaagggc agcccaagag
      541 gtaatgaaac tcaccgggac cgacaccata gccgttaagg ctcaaattca tgccggtggc
      601 cgtggcaaag gcggtggcgt taaaattgcc aaaacggttg atgaggttgc aacctatgca
      661 tcgaatataa ttgggatgaa cctcgtcacc catcaaacca gccctcaagg taaacttgta
      721 cgaaaggtgc tcattgagca aggtatttat caacctggcc cttcggagat caaggagtac
      781 tacatgagta ttctgttaaa ccgggcaact ggccggcata tggtaatata ctccccccag
      841 ggaggaatgg acattgaagc tgtagctgct caaactcctg agctaatctt caaggaagaa
      901 attcacccac aaggtcttca ggcatttcag gctcgtaaaa tagcatttaa tctgggatta
      961 tcaggcaaag cctaccaaca aatggttaag tttgttatgt cgctctacga agcctatacc
     1021 aaaaacgacc tttcgttgct tgaaataaac cctgttgtaa aaacatcgaa cgacgatatt
     1081 ttggccgctg atgccaagtg tagtattgat gataatgccc tttaccgtca tcccgattta
     1141 gctgaatacc gcgatataaa cgaggaagac cctgctgagg tagaggctgg agagcacaac
     1201 cttaactttg ttaaactcga tggtaatgtt ggctgcatgg taaatggcgc tggtttggct
     1261 atggcaacca tggatattat taaactctcc ggcggcgatc ctgccaactt ccttgatgta
     1321 ggtggcggcg caaatgcaaa aaccgttgcc gcaggtttca ggataatact caaagattct
     1381 aacgttaaag ccatacttat caatattttt ggcggtattg tacgttgcga ccgagtagca
     1441 caaggtgtag ttgatgcata caaggaaata gggaatatac ccgttccagt tattgttcgt
     1501 ttacagggaa ccaacgcctt agagggtaaa aaaatgatcg acgaatccgg attaaaggtt
     1561 tacagcgcca ttacattgca ggaggcggct aatctggtaa agcaaatggt aaactagtaa
     1621 caaataacgg ggacaagccc cgttctttgt tacttcttgg atgagcaggt attaatccct
     1681 aacagggagt gaagcccaca cgtccatgtc aatgcgttag caaggggaac tatagccaat
     1741 aaaccccacc agctctgata gtagattctt actccagcaa taaccagggc tatcaggatt
     1801 cagattacct tatcaatttt gcctacattt ttttccattg tttcaaagtt taatgattgc
     1861 ttttcttgaa acaaatcaca atggaaaatg tttactacca ctttattggt ttgcgacgga
     1921 aaggcatagt cataca
//
LOCUS       DAOOWJ010000214         1916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_60056_length_1916_cov_4.820527, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000214 DAOOWJ010000000
VERSION     DAOOWJ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60056_length_1916_cov_4.820527"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            497..>1916
                     /gene="pruA"
                     /locus_tag="PLW22_12070"
     CDS             497..>1916
                     /gene="pruA"
                     /locus_tag="PLW22_12070"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOU75482.1"
                     /translation="MNNAIFKYDLPKNEPVLSYLPCSPEREALEKEIKRLSSSVMDIP
                     LIIGGKEVRTGKLGKVVMPHNHSHVLAQYHIAGEEETQMAIEAALKAHEYWSQVSWVE
                     RLSITLKVAELISKKYRATLNAATMLGQGKNVYQAEIDAACETIDFLRFNAHYISEIY
                     NQQPISETRTINRLEYRPLEGFVFTVSPFNFTAIASNLNMAPVLMGNTVVWKPASTAL
                     LSNYILMQIYKEAGVPDGVINFIPGKGSAIGNVVFNHPMLAGIHFTGSTATFNTFWKA
                     VSNNLAKYRSYPKLVGETGGKDFIFVHPSANINQAVIAAVRGAFEYQGQKCSAASRAY
                     IPESLWPEFKKNILGIMHEINMGDPTDYETFVNAVIDEASFDNIMSYIEKAKQSDAAE
                     IIAGGKGDKSKGYFVEPTIIVTTDPHFVTIEEEIFGPVLTIYVYPDEKFEETLELCDK
                     TSPYALTGAIFSSDRQALNKACR"
BASE COUNT          613 a          413 c          407 g          483 t
ORIGIN      
        1 cccaccgcca cgttccaccc ccgacccatt gccgggtcag gcttttatgc cactgtgttt
       61 gactctccgt attggttaag gaattgcata aaagaagagt tgaaggcact aagagatgct
      121 cagcttgaca aaggtttcac aaacaacgaa caacgaacaa cgagcaactt acacgaataa
      181 cttcatttgt catcctgagc gtagcgaagg atctctaatc gacccattaa gatgtttcgc
      241 ttgcgcgcaa catgacaaat aaccttaact aaacaacatt gattaataca ttaaaaaaat
      301 ctcgcaagat gtctataaaa gacatgcttt accggaaatt ctgttgaaat tgattaattc
      361 ctcaaaacaa tttgaatttc tcattgtttt attatttaga acgattccat ataggtgatt
      421 tttgataaaa gtcatttcaa gaaacaaccg ttatgcattt ttttgcatat cgaaaacaaa
      481 aaaaacatta aaacctatga acaacgcaat ttttaagtac gatttaccaa aaaacgaacc
      541 ggtgctcagc tacctgcctt gctcaccaga gcgcgaagca ctggaaaaag aaattaaacg
      601 gctatcatct agtgtaatgg atataccgct tattataggt ggtaaggagg ttcgaacagg
      661 gaagttaggt aaagtggtta tgcctcacaa tcacagccat gttttagccc agtaccatat
      721 tgcaggcgag gaagaaaccc aaatggccat tgaggctgcg ctcaaggcgc atgagtactg
      781 gtcgcaggta tcgtgggttg agcgcttgag catcacatta aaagtggccg aactgatatc
      841 aaaaaaatat cgagccacgc taaatgcagc aaccatgctt ggacagggca aaaacgttta
      901 ccaggctgag attgatgccg catgcgaaac aattgatttt ttgagattca atgctcacta
      961 tatctccgag atttacaacc agcaacccat ttcagaaacc cgaacaataa acaggttgga
     1021 atacaggccg ctggaaggat ttgtttttac agtaagccca ttcaacttta cggctatagc
     1081 atcgaacctg aatatggcac ctgtgctgat gggtaatact gtggtttgga aaccagcttc
     1141 aaccgcacta ctttcgaact acatactcat gcaaatatac aaggaggccg gagttcccga
     1201 tggtgtaatc aactttatac cgggcaaagg ctctgccatt ggcaatgtgg tgttcaacca
     1261 tccaatgcta gcaggaatcc actttaccgg ttcaacagca acattcaaca cattttggaa
     1321 agcggttagc aacaacttag ccaaataccg ttcgtatccc aaactagttg gcgaaactgg
     1381 tggtaaggat ttcatttttg ttcaccctag cgccaatatc aaccaggcag tgattgctgc
     1441 cgttcggggg gcttttgagt accaggggca aaaatgctca gcagcatcga gggcatacat
     1501 tccagagtca ctttggccgg aatttaagaa aaacattcta ggtattatgc atgagatcaa
     1561 catgggtgac cctaccgact acgaaacatt tgtaaacgca gttattgacg aggcatcgtt
     1621 cgacaatatt atgagttaca ttgaaaaagc aaagcaatcc gatgctgctg aaataattgc
     1681 cggaggcaag ggcgataaaa gcaagggtta ctttgtggaa ccaacaatca tagtaacaac
     1741 cgacccacac tttgtgacga tagaagagga aatatttggc ccagtgctga ccatatacgt
     1801 ttaccccgat gagaagtttg aggaaacgct tgagctttgc gataaaacct caccgtatgc
     1861 gcttacgggt gcaatattct cgtccgatag gcaagcattg aataaggcat gtaggg
//
LOCUS       DAOOWJ010000215         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_62103_length_1872_cov_6.314254, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000215 DAOOWJ010000000
VERSION     DAOOWJ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62103_length_1872_cov_6.314254"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1872
                     /locus_tag="PLW22_12075"
     CDS             15..>1872
                     /locus_tag="PLW22_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU75483.1"
                     /translation="MFFSNMVKRFTKWSHKGYGVFESFRTVVTIGVLPFSYCLLAMPM
                     ATFAQPDSVAVTKNVDIEEVVVNARKKASTYSELTRVVRVVNRDELGQRKATALGELL
                     EQVAAIDIRQRGANGVQADINFRGGSFDQVMVLLNGINITDPQTGHHNLNIPINIDAV
                     DRIEILQGPGAREYGVGAFAGAINIITKPDSNSSGQLSFFAGQHGLAKVSGSQNFGTK
                     KLRSFVATSFDRSDGYIENTDFKNLNIYLHTSYTLDKAELNFFAGYQDKGFGANSFYT
                     PKYPNQYEATKTILGSVSLDKSWKNYALKTAGYYRKHTDRFELFRSNPAVWYTGHNYH
                     ATDIYGAKLSVSRFYRWGKTDAGLELRREEILSNKLGTPLTDSVKVWGEDVYYKKGDS
                     RNHLMAFASQSFFFDQFSFSFGGQFNHSNKFGDIWTWGADLSYDLYQHARPFVSVNRS
                     FRLPTFTDLYYQGPTNIGNPNLKAEFATTYESGIKMDRNRLMAEVALFYRQGSDIIDW
                     VKPANETVWTTVNYTKLNSWGANVNFSVNTKNLNIYTDRITLSYAFTESEKGKSELDS
                     YYVLDYLRHCLTLSSQHTFKNLFLNWTIRYQDRNGEYLKYIDTNTSIPIAYSN"
BASE COUNT          550 a          365 c          422 g          535 t
ORIGIN      
        1 ctatgcgtaa aaaaatgttt ttcagtaaca tggtaaagcg attcacaaag tggtcgcaca
       61 agggttacgg tgtttttgag agtttcagaa cagttgttac tatcggagtt ttaccatttt
      121 cgtattgcct gcttgccatg cccatggcaa cctttgccca gcccgatagc gttgcggtaa
      181 ccaaaaatgt tgatatcgaa gaggtagttg ttaatgctcg caaaaaggcg agtacctatt
      241 cggagcttac cagggtagta agggttgtta accgcgatga gctggggcaa cgtaaggcca
      301 ctgcgcttgg tgagttactc gagcaagtgg cggcaattga tatcaggcag cgcggagcaa
      361 atggtgttca ggccgacatt aatttcaggg gtggctcgtt cgaccaggtc atggtattac
      421 tgaatggcat caacattacc gatccgcaaa ccggccatca caacctaaac attcccatta
      481 atattgatgc ggtagatcgg atcgaaattc tgcaaggtcc aggggctaga gagtacggtg
      541 ttggggcttt tgctggtgca attaacatta ttactaagcc cgatagcaat agtagcggtc
      601 aactttcctt ttttgcagga caacatggct tagccaaagt gtcgggttcg caaaattttg
      661 gaactaaaaa attacgttcc tttgttgcaa catcgttcga taggagcgat gggtatattg
      721 agaataccga tttcaaaaat cttaatatct accttcacac atcttataca ctcgataaag
      781 cagagcttaa cttttttgca ggatatcagg ataagggttt tggggctaat agtttttata
      841 cgcctaaata tcccaaccag tatgaggcta ccaaaaccat cttaggttca gttagtcttg
      901 ataaatcatg gaaaaattat gcattgaaaa cagctggtta ttaccgtaaa cataccgaca
      961 ggtttgaact tttccgaagc aatccggcag tgtggtatac cgggcataac taccatgcta
     1021 ccgatattta cggagcaaaa ctttcggtaa gtcgttttta ccggtggggt aaaaccgatg
     1081 cagggcttga attaaggcga gaggaaatac tgagcaataa gctcggaacc cctttaaccg
     1141 attccgttaa ggtttggggt gaagatgttt actacaaaaa aggcgattcg cgtaatcacc
     1201 ttatggcttt tgccagtcaa agcttcttct ttgatcaatt ctcattctcg tttggcgggc
     1261 agtttaatca ttccaataag tttggcgata tctggacctg gggtgccgat ttgtcatacg
     1321 acctgtatca gcacgcaagg ccttttgttt cggttaaccg atcgttcagg ttgccaactt
     1381 ttaccgattt atattaccag ggcccaacca atattggcaa tccaaaccta aaagcagagt
     1441 ttgccactac ctatgaatcg ggtataaaaa tggatcgaaa tagattgatg gctgaggttg
     1501 cattatttta caggcagggt agcgatatta ttgactgggt aaaaccggca aacgaaacgg
     1561 tttggacaac tgtaaactat actaagctta actcgtgggg tgcaaatgtc aactttagtg
     1621 tgaatactaa aaatcttaat atttacaccg ataggattac tctatcgtat gcatttaccg
     1681 aaagcgaaaa gggtaaaagc gagcttgatt catactacgt gcttgattat ttaaggcatt
     1741 gcttaacatt gagttcgcaa cataccttta aaaacctttt cctgaattgg actataaggt
     1801 atcaggacag aaatggggag tatttaaaat atatcgatac aaatacatca attccaatag
     1861 cttattccaa cg
//
LOCUS       DAOOWJ010000216         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_65015_length_1811_cov_4.762528, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000216 DAOOWJ010000000
VERSION     DAOOWJ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65015_length_1811_cov_4.762528"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1811)
                     /locus_tag="PLW22_12080"
     CDS             complement(<1..>1811)
                     /locus_tag="PLW22_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU75484.1"
                     /translation="ENYPTSIDFFEKAANHYEKAHSYKNYVRAKGNIGLCLVKMGEKD
                     SAKKILFSTLGNYGKYMDSLMLASFFDNIGVLYEALGKIDSALHYHTHSLNISKKLKS
                     SRGIALSNLAIGRCYNGQGKYIVARTHLLTAKQVSKTNELEEITAQANRQLATCYEEL
                     GDFRSSQRCLKEFLEYNDKTFTKKMVNQVAQLHSMLQIEKQEKENQQLQLQLELKSLN
                     EQKDQKIITLYTIFSILLLLTLSVIVYFAIQLSHKKKLLEHTNSQLFKFNNELDSLVK
                     HRTRELNDAIDKIRELERIKSAFMANISHEIRTPLNGILGLTYYLSTPECTAEERSQI
                     GEQVKHLGSKLARIVDDILELSKIETNQVNLLLSEVNLNQLLDDVYKEYDSITEYDDK
                     NLFFRVNKSLPNDRAIVICDYYRLRSIIIHLLENAFKFTHQGGIEMGYSINENSVLHL
                     YVKDTGVGIPNEIQKKVFERFYKHLDDSNPIFYDGLGIGLTIAMGYAIALGGSIKLQS
                     SPGQGSTFTLVLPITVPNSNANKETFSFTGKRILVVEDDLINYQYLHALLSKTGATVI
                     HVKNGEDAIEAATIERAADLILLDIQLPFKSGIEAAI"
BASE COUNT          483 a          352 c          363 g          613 t
ORIGIN      
        1 tcaatggcag cctctattcc gcttttaaaa ggaagttgta tatccagaag aattaaatcg
       61 gctgcccttt caatggtggc tgcctctatg gcatcttcgc catttttaac atgaataaca
      121 gtagcaccgg ttttgctaag caaggcgtgt aggtactggt agttaatcag atcatcctct
      181 accaccagta ttcttttacc ggtaaagctg aaagtttcct tgttggcgtt agagttgggt
      241 accgttatag gtaatacaag agtaaaagtt gatccttgcc ccggcgagga ttgaagtttg
      301 atgctacctc ctaatgcaat ggcataaccc atggctatgg ttaatccaat tcctaaacca
      361 tcgtaaaata ttgggttgga atcatctaag tgcttataaa agcgctcaaa tacttttttc
      421 tgaatttcat tgggtatgcc tactcccgta tctttgacgt aaaggtgcag cactgagttc
      481 tcgtttatgg agtaacccat ttcaatacca ccctggtgcg taaacttaaa agcattctct
      541 aaaagatgaa taataatgct ccgtaaacgg tagtaatcgc atattactat tgccctatcg
      601 ttgggtagcg atttgttcac cctaaaaaag agatttttat catcatattc agtaatggag
      661 tcatactcct tgtaaacatc atcgagtaac tgatttaggt tgacctcgga cagcagaagg
      721 tttacctgat tagtttcaat ttttgaaagt tcaaggatat cgtcaacaat cctagccagt
      781 ttgctgccta agtgtttaac ctgttcgcct atctgcgagc gctcttcggc tgtgcattcc
      841 ggtgtcgaaa ggtagtaggt gagtcccagt atcccattca ggggcgttcg aatttcatgg
      901 ctgatatttg ccatgaatgc ggatttaatt ctttcaagtt cccttatttt atcaatagca
      961 tcattcagtt ccctggttct gtgttttacc aaggaatcga gctcgttatt gaatttgaac
     1021 agctgcgaat tagtgtgttc cagtagtttt ttcttgtgag agagttgaat ggcaaagtaa
     1081 acaattacgc taagggttaa aagcaaaaga atagaaaaga ttgtgtataa agtaattatc
     1141 ttttgatcct tctgctcatt taacgatttt agttcaagct gcagctgtag ttgttgattc
     1201 tccttttctt gcttttcaat ctgcaacatc gagtgcaatt gagcaacctg attcaccatt
     1261 ttcttggtga atgttttatc gttatactcc aaaaactcct ttaggcatct ttgagaactt
     1321 ctaaaatcgc caagttcttc gtaacaagtg gcgagctgcc tgttggcctg tgcagtaatt
     1381 tcttctagtt cgtttgtttt ggatacttgt ttggcagtaa ggaggtgtgt ccttgctaca
     1441 atgtatttgc cctgaccgtt gtagcatcgg ccaattgcca agttgcttaa tgcaattcct
     1501 cgtgatgatt taagtttttt actaatattt aatgaatggg tgtggtagtg aagtgccgaa
     1561 tcgattttgc ctaaagcctc gtatagaact ccaatgttat caaaaaacga tgcaagcata
     1621 agcgaatcca tatacttgcc ataattgcca agcgtactaa ataaaatttt tttagcacta
     1681 tccttttcgc ccatcttaac aaggcataat ccaatatttc ctttagcccg tacatagttt
     1741 ttgtaactgt gagctttttc gtagtggttt gctgctttct caaagaaatc gattgatgta
     1801 gggtagttct c
//
LOCUS       DAOOWJ010000217         1746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_68462_length_1746_cov_3.594914, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000217 DAOOWJ010000000
VERSION     DAOOWJ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68462_length_1746_cov_3.594914"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PLW22_12085"
     CDS             complement(<1..1345)
                     /locus_tag="PLW22_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75485.1"
                     /translation="MFKKIINRFLAILLVVSALWACETDNFSSSPSIKLTFSADTVLF
                     DTVFTTIGSSTRYLKVYNTGKSDVRISSVALAGGSSSAYRINIDGRPGPVVNEIPLRS
                     GDSLFVMVEVTVNPTLSDAPLLIPDSIVFVTNTNVQNVKLIAWGQDVYMHNGVTIEND
                     SVFPVNKPHLIYDYLFVDKNATLTIPAGARLHFHNNAQLEVAGTLKVEGSADLPVTMQ
                     GDRLEKFYSDKGGQWGGVWLRAGSRYNSIAWADIKNAIIGLMVDTCFTPGVPTLVLSN
                     SKIGNSSYVNLLTRGAKLEVDNCLFHNAAYSSVALTLGGAYRFYHTTISNYWGDYMFR
                     KGPALLINNYYTYQLVLGGPILLEPRNLDEASFFNSIIYGSISNELGLDYKYNGQPVN
                     ANFNYNFTDCILRVPPSINLNDPEHFMRVKRDDPRFKDIAKWNFELDTLSPAKDIG"
     gene            1408..>1746
                     /locus_tag="PLW22_12090"
     CDS             1408..>1746
                     /locus_tag="PLW22_12090"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU75486.1"
                     /translation="MKTHSILIFLTISIAIMACSKRQKADLLVTNATVYTVDSTFSVA
                     ESFAVDNGKIVAVGKNDEILSAYRADTILDLKGKFVYPGFIDAHCHFLGYGLGLPKAW
                     LAGAKSWSEVV"
BASE COUNT          516 a          370 c          390 g          470 t
ORIGIN      
        1 cacctatgtc cttagctggg ctaagggtat cgagctcaaa attccacttg gcaatatcct
       61 taaacctggg gtcgtcgcgc ttaacacgca taaaatgttc aggatcgttt aggttaatac
      121 ttggtggcac cctaagaatg cagtcggtaa aattgtagtt aaagtttgca ttaacgggtt
      181 gaccgttata cttgtaatcc agtcctaact cattggatat gctaccataa ataattgaat
      241 taaagaatga tgcctcgtcc aaattgcggg gttcaagcag aatcggcccg cctagtacaa
      301 gctgataggt atagtaattg ttgatcagta atgctggccc tttacggaac atgtagtcgc
      361 cccagtagtt agatattgtg gtatggtaaa accggtatgc ccccccaagg gtaagagcaa
      421 cacttgagta ggcagcattg tgaaaaaggc agttgtcaac ctccaacttt gccccacggg
      481 taagcaggtt aacgtaactt gaattgccta ttttggagtt tgagagcaca agggttggta
      541 cacccggggt aaagcaggta tcaaccatta acccaataat tgcattctta atatcagccc
      601 atgcaattga gttatacctg gaacctgctc tcaaccacac cccaccccat tgtccaccct
      661 tgtcggagta aaacttttcc aacctatcgc cctgcatggt tactggtagg tcagccgaac
      721 cttctacctt tagggttccc gctacctcga gttgtgcgtt gttatggaag tgtaatcgtg
      781 cgccagcagg gatggtaagg gtagcattct tatccacaaa aaggtaatcg taaatcagat
      841 ggggtttatt aacgggaaaa accgaatcgt tttcgatggt taccccgtta tgcatgtaaa
      901 catcctgccc ccaagcaatc agcttaacat tctgaacgtt agtattggtt acaaaaacaa
      961 tagaatcggg tattaacagc ggagcatcgc ttagcgtagg attaacggta acctcaacca
     1021 taacaaaaag gctatcgccc gaacgtaaag ggatttcatt caccacaggg cctgggcgtc
     1081 catcaatgtt tatcctgtac gccgatgaac tgccaccagc taaggctact gatgaaatgc
     1141 gaacatcgct tttgccagtg ttgtaaacct tgaggtaacg ggttgatgaa cctatggtgg
     1201 taaacacggt atcgaacagc accgtatcgg cgctaaaggt gagcttgatt gatgggctgg
     1261 acgaaaagtt atcggtttcg catgcccata atgctgacac gacaagtagg atagctaaaa
     1321 atcgatttat tatcttttta aacatcagca acaaaataat tttctacttt tgataatgaa
     1381 actcacagca aagatagttt aactgacatg aagacacact caatattgat atttttaacc
     1441 ataagcatag caattatggc atgtagcaaa cggcaaaaag ccgatttact tgtaaccaat
     1501 gcaactgttt atacagttga ttcaacattc tcagtagctg aaagttttgc agttgataat
     1561 ggtaaaatag tagcagtggg taaaaacgat gagatactct cagcatatag agccgacacc
     1621 atacttgacc taaaagggaa attcgtttat cctggcttta ttgatgccca ctgccacttt
     1681 ttagggtatg ggttaggtct gcccaaggca tggcttgcgg gagcaaaatc gtggagcgag
     1741 gtggtt
//
LOCUS       DAOOWJ010000218         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_73377_length_1661_cov_7.632005, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000218 DAOOWJ010000000
VERSION     DAOOWJ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73377_length_1661_cov_7.632005"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLW22_12095"
     CDS             complement(<1..473)
                     /locus_tag="PLW22_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOU75487.1"
                     /translation="MEKTYFAHETAVIDEGCTIGKGTKIWHFSHIMSGCEIGESCNIG
                     QNVVVSPGVKLGRNVKVQNNVSIYTGVICEDDVFLGPSMVFTNVINPRSAINRKSEYL
                     TTLVKRSATIGANATIVCGVTLGEFSFVGAGAVVTKDVKPYALVVGNPARQIGWM"
     gene            complement(495..1628)
                     /locus_tag="PLW22_12100"
     CDS             complement(495..1628)
                     /locus_tag="PLW22_12100"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU75488.1"
                     /translation="MNIQMCDLRGQYLKIKSEIDAAIQEVIDSTAFIKGKEVGLFQEE
                     LASYLGVKHVIGCANGTDALQIALMALDLKPGDEVITPDFTFIATVEVVALLGLTPIL
                     VDVDPSTFTIDTKRLEEAVTPKTKAIIPVHLFGQCANMDEIMRLAQKYNLSVIEDTAQ
                     ALGADYYHSNGRVSKAGTIGTIGTTSFFPSKNLGCYGDGGALFTNDDSLANEIRSIAN
                     HGMKVRYHHDRIGVNSRLDTIQAAILRVKLKYLDFYNKARIEAANAYDSALKPCSKII
                     TPARAKWSSHIFHQYTLKLNNVDRERLQKDLAAKGIPTMVYYPIPLHKQKAFEPYREF
                     NYGRSFQVSDFLSSSVISLPMHTELSADVIKYITETLLETLKD"
BASE COUNT          468 a          370 c          340 g          483 t
ORIGIN      
        1 ctcatccaac ctatttgccg ggctggatta ccaacaacca gagcgtaagg ctttacgtcc
       61 ttagttacca ctgctccggc accaacaaag gaaaactcgc caagggtaac gccgcatact
      121 atggttgcat ttgctccaat ggtggcgcta cgctttacca gcgttgttaa atactcactc
      181 ttgcggttaa tggcgctacg cggatttatt acattggtaa acaccattga tgggcccaaa
      241 aaaacatcat cctcgcaaat tacacccgta taaatggaaa cattgttctg aacctttaca
      301 tttcggccta attttactcc cggggaaaca accacatttt gcccaatatt gcaactttca
      361 cctatctcgc agccagacat tatatggctg aagtgccata tttttgtacc cttgcctatt
      421 gtgcaacctt catcgattac tgctgtttcg tgggcgaagt aggttttctc catgacaata
      481 aaaatttatc aaaattaatc ttttaatgtc tccagtagag tttcggtgat atattttatt
      541 acatcggctg atagctcggt atgcatggga agcgagatta cagaacttga tagaaaatcg
      601 gaaacctgaa acgaacgccc ataattaaac tccctgtagg gttcaaaagc tttttgctta
      661 tgcaacggta ttgggtagta aaccatggta ggaatacctt tggcagccaa atctttttgt
      721 agcctttcgc gatcaacatt gttaagcttt aatgtgtact gatgaaagat atggctactc
      781 cacttagctc gtgccggcgt aataatcttg ctacaaggtt ttaacgcgga atcgtaggca
      841 tttgcagcct caatacgagc cttattataa aaatcgaggt acttcaactt aacccttaaa
      901 atggcagcct gaatagtatc gagacgcgag ttaactccta ttcggtcgtg gtgataacgc
      961 accttcatcc catggttggc aattgaacgt atctcgttag ccaagctatc atcattggta
     1021 aaaagagcac ctccgtcgcc atagcaacct aagttttttg aagggaaaaa cgatgttgtg
     1081 ccaatagttc ctatggttcc tgcttttgaa acccggccat tggagtggta ataatcggca
     1141 cccaatgcct gtgcggtgtc ctcaataacg gataggttat acttttgagc taaacgcatt
     1201 atctcatcca tgttggcgca ttgaccaaaa agatgaacag gaataatggc tttggttttt
     1261 ggggttacgg cttcctctaa ccttttagta tcgatagtaa aggttgatgg atcaacatca
     1321 acaagaattg gggtaaggcc aagaagtgca actacttcaa ccgtggcaat aaaagtaaag
     1381 tcgggcgtga taacttcatc gcccggtttt aaatcgagtg ccattagtgc tatttgaagg
     1441 gcatcggttc cgttggcaca gccaattaca tgcttaacgc ccagatagga agcaagttct
     1501 tcctgaaaca gacctacctc tttccctttg ataaaagcag tactgtcaat tacctcctga
     1561 atggcagcat caatctcact ttttatcttt aagtactgcc cccttaggtc gcacatctgg
     1621 atgttcatag atttaaggtt aaaggttcaa agttcaagga t
//
LOCUS       DAOOWJ010000219         1468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_87322_length_1468_cov_4.479830, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000219 DAOOWJ010000000
VERSION     DAOOWJ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87322_length_1468_cov_4.479830"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="PLW22_12105"
     CDS             <1..1364
                     /locus_tag="PLW22_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOU75489.1"
                     /translation="KGFYCEIDGCNLPLELPLIFNICDRMREIVAADLPFRRDKVLTS
                     DAIKLFRDLGYEDKARLFETRPTLYTSVYYLGDMADYFFGSLVPSTGYLKVFDLTKYY
                     DGMLLRVPKRFQPDEVEDVVIQNKMFEIFQEYKDWLEILGVENVGAINQEVAKGNASE
                     LIKISEALHEKKVADIADRIHQQPEPVRLVLISGPSSSGKTTFAKRLAIQLKVNGLKP
                     YTISLDNYFVDREKTPKDEFGNYDFEALDALDVEYFNNDLVRLLKGEEVEIPKFSFEE
                     GKRFFDDTKLQIEPNGVIIIEGIHALNPALTHLIPNNQKFKVYVSALTSISIDGINRI
                     PTTDNRLIRRIVRDYRYRGYSALDTIGRWESVRRGEDKNIFPFQEEANVMFNSALLYE
                     FSVLKRFAEPIIQEVPPTRPEYDEAQRLLKFLSYFTPISEREVPPTSIIREFLGGSTF
                     SYK"
BASE COUNT          431 a          311 c          323 g          403 t
ORIGIN      
        1 ccaaaggctt ctactgcgag attgacggct gcaacctgcc cctggaatta ccgcttatat
       61 tcaacatatg cgatagaatg cgcgagattg tggcggcaga tttgccattt cggcgcgata
      121 aggtattaac atccgatgct atcaaactct ttagggattt gggttacgag gacaaagcca
      181 ggttattcga aactcgtcct accctttaca cctcagttta ctaccttggc gacatggccg
      241 attacttctt cggaagtctt gtcccttcaa cgggctacct aaaagtgttt gacctcacta
      301 agtattacga tggaatgctg ctgagggtgc ctaaacggtt ccagcccgat gaagtggaag
      361 atgtagttat tcagaacaaa atgtttgaaa tctttcagga atataaggat tggcttgaga
      421 ttttaggcgt tgagaatgta ggagctatta accaagaggt agccaaagga aatgccagtg
      481 aactaataaa aatttcagag gcattacatg aaaaaaaggt tgccgacatt gccgatagaa
      541 tacaccagca acccgaaccg gtaaggcttg tacttatttc tggcccatca tcatcaggta
      601 aaacaacttt tgctaagcgt ttagcaattc agctaaaggt taatggttta aaaccataca
      661 ccatctcgct cgataactat tttgttgaca gggagaaaac ccctaaggat gagttcggaa
      721 actacgactt tgaggcactg gacgcgcttg atgttgagta ttttaacaat gacctggtca
      781 ggctgcttaa gggcgaagag gtggaaatac caaaattctc ctttgaggag ggtaagcgct
      841 tttttgatga caccaaactt cagattgaac ccaatggcgt aataataatt gagggtattc
      901 atgccctaaa cccagcatta acccacttaa tccctaataa ccaaaagttc aaggtttacg
      961 tttcggcatt aacctcaatt tcaatcgatg gcataaaccg aataccaacc accgataacc
     1021 gattaattcg cagaatagta agggactatc ggtatcgggg gtatagcgcg ctggatacta
     1081 ttggcaggtg ggaaagtgtt cgtcgtggcg aggataaaaa catcttccca ttccaggagg
     1141 aggccaacgt tatgtttaac tctgccctgc tttacgagtt tagcgtttta aaacgctttg
     1201 ctgaaccaat aatccaggag gttccgccaa cccggccaga atacgatgag gcccaacggc
     1261 tgcttaagtt cttaagctat ttcactccaa tatctgaacg cgaagtgcca cccacctcaa
     1321 tcatacgtga gtttctggga ggtagcactt tttcctataa atagcacaaa gttttcaaca
     1381 gtgtattatg ctgatttatt gccgtttgca tattttttgt tagtaacaat tactcgtttg
     1441 aaagcccaaa agattagatt tttgggct
//
LOCUS       DAOOWJ010000220         1372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_96564_length_1372_cov_2.921792, whole genome shotgun sequence.
ACCESSION   DAOOWJ010000220 DAOOWJ010000000
VERSION     DAOOWJ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96564_length_1372_cov_2.921792"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PLW22_12110"
     CDS             <1..1241
                     /locus_tag="PLW22_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU75490.1"
                     /translation="VNPNASPQEMENARQKIVDIHNRLLNGENFAELAKKESQDNSTA
                     PNGGELPWIRSGQIIPEFEEVAFALKDIGDISKPFQTSFGWHVVKLLDRKLPGEFEEM
                     LPEIKSLMARDSRSMLIRSSFIKKLKKEYGVEEFPAESAKHLSSLIDSSIFKGNWKAP
                     NIKTNPTLLVVKGLNYTLSDLLQFIGQNQQKSKGNSLQSFISSIYTDWVNQTIINFEK
                     SLLPQKYPEFKQLANEYLEGMLLFEISNREVWTKASDSVAISTFYENHRDKYTWGEKI
                     YFTVYQTPDSKIAQAFENALKKNPAINPDVLIKKWNKKTQRVTYNQQVGFPDNPIISG
                     YTQWNSGINISVQNDKHVVVHINKTTTGEPKELNDCRAEVMADYQQYLEESWIQTLKE
                     KYKVNINNDTYNKLLENLNN"
BASE COUNT          472 a          312 c          267 g          321 t
ORIGIN      
        1 gggttaaccc caatgcctcg cctcaggaaa tggaaaacgc ccgccaaaaa atagtcgaca
       61 ttcataaccg cctacttaac ggcgaaaact ttgccgagtt agccaaaaag gaatcgcagg
      121 acaacagcac agccccaaac ggtggcgaac taccctggat tcgttccggg caaataatac
      181 ctgagttcga ggaggttgct tttgccctaa aggatattgg cgatatctcc aagcctttcc
      241 aaacctcatt tggatggcac gtggttaaac tacttgatag aaagctaccg ggcgagttcg
      301 aggagatgct gcccgaaatt aaaagcctaa tggctcgcga cagccgatcc atgctcatac
      361 gctcctcatt cattaaaaag cttaaaaaag agtacggggt cgaggagttc cctgccgaat
      421 cggccaagca tctctcatcg cttatcgatt cctccatttt taaaggcaac tggaaagctc
      481 caaacatcaa aaccaatcca actttactgg ttgtaaaagg gttgaattat acactatcgg
      541 acttgcttca gtttatcggc caaaaccagc aaaaaagcaa gggcaattcc ttacagtcgt
      601 tcatcagttc tatctatacc gattgggtaa accaaaccat aattaacttt gaaaaatcgc
      661 ttttacccca aaaatatcct gaatttaaac agcttgcaaa cgaatactta gagggtatgc
      721 tgctttttga aatctcgaac cgcgaggtat ggaccaaagc ctcagatagc gttgcaatta
      781 gtacatttta cgagaatcac cgcgacaaat acacatgggg cgaaaaaatt tactttaccg
      841 tgtaccaaac ccctgatagc aaaattgccc aggcgtttga aaatgcacta aaaaagaacc
      901 cagccataaa tcccgatgtg cttattaaaa aatggaataa gaagactcaa cgagttacct
      961 acaaccaaca ggtaggtttc cccgataatc ctataatttc aggttacacc caatggaact
     1021 cggggattaa cataagcgtt cagaacgaca aacatgttgt agtacatatc aataaaacaa
     1081 ctaccgggga gccaaaggaa ttaaacgact gcagggcaga agttatggct gactatcagc
     1141 aatatttgga agaatcatgg attcaaacgc ttaaggaaaa atacaaggtg aatattaaca
     1201 atgacaccta caataagtta cttgaaaatc tcaacaacta aaaaaatgaa cagactcctt
     1261 ttaacgctga ttgtagtaac tggctttctt actgcttgcc aaaacgacga taataggcgg
     1321 cctgtagcaa gggtatacga taagtaccta tacgaaagcg accttcaaaa ta
//
LOCUS       DAOOWJ010000221         1306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_103833_length_1306_cov_11.338129, whole genome shotgun
            sequence.
ACCESSION   DAOOWJ010000221 DAOOWJ010000000
VERSION     DAOOWJ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103833_length_1306_cov_11.338129"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..807)
                     /locus_tag="PLW22_12115"
     CDS             complement(259..807)
                     /locus_tag="PLW22_12115"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOU75491.1"
                     /translation="MKLSKKLFLILCISLSLGTTSVYAQRYASRNGTVKLSYNAPHGK
                     METVNRQVNVGLNVETGEFRLTMLMLSFRFQSAYNQEKFNEYFIKNAHFANSTFRGKI
                     VDLHKVNFSKKGEYEVTVTGDLTLRQVTNKVTAKGKFIVGDNTFSGKSVFKVNLMDFN
                     FNIPPTMEKLIEVTFDVDLKKL"
     gene            complement(819..1247)
                     /locus_tag="PLW22_12120"
     CDS             complement(819..1247)
                     /locus_tag="PLW22_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU75492.1"
                     /translation="MKTILKILLILLAIIVIAALGFYFFVYNKPHVDYSKSDVEIEIK
                     AKELFSDFVKNPLAAAKKYNGKVLMVSGTVDAIEQAGELTIAIMIMDEGFFGPEGVRF
                     SLLENQVNTIDIGKPVNLKGLCTGYTEADVVLEHVSVVAQ"
BASE COUNT          375 a          271 c          264 g          396 t
ORIGIN      
        1 caaggaagaa atggtgagcg tgttgtacgt aagatggtta agaactaatt cttactttta
       61 gatatgaaga accccggtac cagccggggt ttttttatgt ttctacaaga gcgcatattc
      121 attactcaat tctcatatgt ttttcaatga gttactgagt aattgccatg ctcctttgct
      181 gttatatcct tggataaaaa aaataccccc gagagaattt ttaattctga acgggggtta
      241 gtgttaatgg atggaatatt acaacttttt cagatccaca tcaaaagtta cctcaataag
      301 tttctccatg gtgggtggta tattgaagtt aaaatccata aggtttacct tgaaaacact
      361 ttttcccgaa aaggtattat cgccaacaat gaactttcct ttggccgtta ccttgttggt
      421 tacctgccta agggtgaggt cacccgtaac ggttacctcg tattctcctt ttttggagaa
      481 gtttaccttg tgcaaatcaa caattttacc ccgaaatgtg gaattggcaa aatgggcatt
      541 cttgataaag tactcattga atttctcctg attgtatgcg ctttggaatc ggaacgatag
      601 cataagcatg gtcagcctga actcgcccgt ctccacgttc agcccaacgt ttacctgtcg
      661 gtttaccgtt tccattttgc catggggggc attgtagctc agcttcacag tgccattacg
      721 cgaagcgtat cgctgggcgt aaacgctggt tgttccaagc gatagggata tacaaaggat
      781 gagaaatagt ttctttgaaa gtttcatgtt aaaaatattt actgtgcaac aacagagaca
      841 tgttccagca ccacgtccgc ttcggtgtag cctgtgcata gccctttcag attaacgggc
      901 ttgccaatgt caattgtatt cacctgattt tcaagcagag agaaacgaac gccttccgga
      961 ccaaaaaagc cttcgtccat aatcataata gcaatggtta gctcccctgc ctgctcaatg
     1021 gcatcaacag tgccactcac catgagcacc ttgccattat actttttggc agcagccaag
     1081 ggatttttta caaaatccga gaatagttcc ttggccttaa tctcgatttc cacgtcgctt
     1141 ttcgagtagt caacatgggg tttgttgtaa acaaagaagt agaatcctag ggctgcaata
     1201 acgataattg ccagcagaat cagcaaaatc ttaagtattg ttttcatccg caaataatta
     1261 ttaagttaaa caatatcagc atattgaaaa acagataagg agttaa
//
LOCUS       DAOOWJ010000222         1292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_2418
            NODE_105530_length_1292_cov_5.686338, whole genome shotgun
            sequence.
ACCESSION   DAOOWJ010000222 DAOOWJ010000000
VERSION     DAOOWJ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864493
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:03:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,391
            Genes (coding)                    :: 2,386
            CDSs (with protein)               :: 2,386
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105530_length_1292_cov_5.686338"
                     /isolate="AMR_MDS_2418"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLW22_12125"
     CDS             complement(<1..274)
                     /locus_tag="PLW22_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOU75493.1"
                     /translation="MSKPTLAIASDHAGYSTKEELKKYLAELGYNVWDFGTHSDDSVD
                     YPDFAHPLATAIEKGDYQLGILLCGSGNGVSITANKHQGVRSALCWI"
     gene            433..>1292
                     /gene="hemW"
                     /locus_tag="PLW22_12130"
     CDS             433..>1292
                     /gene="hemW"
                     /locus_tag="PLW22_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOU75494.1"
                     /translation="MAGIYIHVPFCRKKCSYCDFYSVGEAKMDNHFTDFVLKELSLRN
                     PLIADRIVKTIYFGGGTPSLLPAGNIKRIISGLGDFFGVDPNAEITIEVNPDDVTLEL
                     AEQYQAIGVNRVSIGIQSFNNDELIFLGRRHDVGKAIEAIRIFRKASIANISIDLIYG
                     LPHSTLKSWEHSLNVAFGLDIQHLSCYHLTYEEGTPLTRRVAKGTVIPLDEELSKAQF
                     DLLCEMSKNNGFIHYEISNLAKPGYISKHNSGYWLGEEYLGLGPSAHSFNRSMRWWNP
                     SSITGWEKEV"
BASE COUNT          380 a          276 c          270 g          366 t
ORIGIN      
        1 gaatccagca taaagccgac cgtacgccct gatgcttatt ggctgtaatt gataccccgt
       61 taccactacc acaaagcaat attcctagct ggtaatcgcc cttttcaatg gcagttgcca
      121 acggatgagc aaaatcaggg taatcaacgc tatcgtcgct gtgggttcca aaatcccaaa
      181 cattataccc cagctcagca agatattttt taagctcctc cttagttgaa tatcccgcat
      241 gatccgacgc aatggcaagt gtaggtttac tcatattagt aacagattca agtttctcta
      301 aaaatacaac gtttcgattt ctgaatacta aattccactt taatcatccc agctaatcat
      361 tacctttgca aagataaagt gtttttatgt tttaagtaca atcatttagc tttgcttaag
      421 caatcggata ccatggcagg aatttatata catgttccgt tttgtaggaa aaaatgcagc
      481 tactgcgact tttactcggt gggcgaagct aaaatggata accattttac cgattttgtg
      541 cttaaagagc tcagcctcag gaacccttta atagctgata gaatagtaaa aaccatttac
      601 tttggaggtg gtacaccatc gctattgcca gctggcaata tcaagagaat tatttccggt
      661 ttaggtgatt tttttggagt tgaccccaat gcagaaatta ccattgaggt aaaccccgac
      721 gatgttaccc ttgagctggc tgagcaatac caagcaattg gcgttaacag ggtaagcatt
      781 ggcattcaat cgtttaacaa cgatgagttg atttttcttg gccgacgaca cgatgttggt
      841 aaagcaattg aggctattcg tatatttagg aaagctagta ttgcaaacat cagcatcgat
      901 ttaatttatg ggctacccca ttcaacgttg aaaagttggg aacactcgtt aaatgtggca
      961 tttgggttag atattcagca cctatcgtgc taccacctta cctacgagga gggaaccccg
     1021 cttacccgaa gggtagccaa gggaactgta attccccttg atgaagagct gagcaaggcc
     1081 cagttcgatt tgctatgtga gatgtccaag aataatggtt tcattcacta cgaaatatcg
     1141 aacctggcaa aaccgggcta tatctcaaaa cacaacagcg gttactggtt gggcgaagaa
     1201 taccttggct taggcccttc ggcacactca tttaacaggt caatgcgatg gtggaatcca
     1261 tcgtccatta ccggttggga aaaggaagtt ga
//
